title: Neural Progenitor Cells in Cerebral Cortex of Epilepsy Patients do not Originate from Astrocytes Expressing GLAST.
abstract: Adult neurogenesis in human brain is known to occur in the hippocampus, the subventricular zone, and the striatum. Neural progenitor cells (NPCs) were reported in the cortex of epilepsy patients; however, their identity is not known. Since astrocytes were proposed as the source of neural progenitors in both healthy and diseased brain, we tested the hypothesis that NPCs in the epileptic cortex originate from reactive, alternatively, de-differentiated astrocytes that express glutamate aspartate transporter (GLAST). We assessed the capacity to form neurospheres and the differentiation potential of cells dissociated from fresh cortical tissue from patients who underwent surgical treatment for pharmacologically intractable epilepsy. Neurospheres were generated from 57% of cases (8/14). Upon differentiation, the neurosphere cells gave rise to neurons, oligodendrocytes, and astrocytes. Sorting of dissociated cells showed that only cells negative for GLAST formed neurospheres. In conclusion, we show that cells with neural stem cell properties are present in brain cortex of epilepsy patients, and that these cells are not GLAST-positive astrocytes.
authors.affiliation: Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Meng
authors.initials: M
authors.lastname: Chen
authors.affiliation: Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Till B
authors.initials: TB
authors.lastname: Puschmann
authors.affiliation: Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Wilhelmsson
authors.affiliation: Department of Pathology, Sahlgrenska University Hospital, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Örndal
authors.affiliation: Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.|Florey Institute of Neuroscience and Mental Health, Parkville 3052, Victoria, Australia.|Hunter Medical Research Institute, University of Newcastle, New South Wales, Australia.
authors.forename: Marcela
authors.initials: M
authors.lastname: Pekna
authors.affiliation: Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Kristina
authors.initials: K
authors.lastname: Malmgren
authors.affiliation: Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.
authors.forename: Bertil
authors.initials: B
authors.lastname: Rydenhag
authors.affiliation: Center for Brain Repair and Rehabilitation, Department of Clinical Neuroscience, Institute of Neuroscience and Physiology, Sahlgrenska Academy at the University of Gothenburg, Gothenburg 405 30, Sweden.|Florey Institute of Neuroscience and Mental Health, Parkville 3052, Victoria, Australia.|Hunter Medical Research Institute, University of Newcastle, New South Wales, Australia.
authors.forename: Milos
authors.initials: M
authors.lastname: Pekny
mesh_terms: D000293:Adolescent; D000328:Adult; D001253:Astrocytes / Q000378:metabolism* / Q000473:pathology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D002648:Child; D002675:Child, Preschool; D000069279:Drug Resistant Epilepsy / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D027341:Excitatory Amino Acid Transporter 1 / Q000378:metabolism*; D005260:Female; D066128:Gray Matter / Q000378:metabolism / Q000473:pathology / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D039902:Multipotent Stem Cells / Q000378:metabolism / Q000473:pathology; D058953:Neural Stem Cells / Q000378:metabolism* / Q000473:pathology; D055495:Neurogenesis / Q000502:physiology*; D055815:Young Adult
chemical_list: D027341:Excitatory Amino Acid Transporter 1; C493394:SLC1A3 protein, human
keywords: GFAP; GLAST; epileptic tissue; human neural progenitor cells; reactive astrocytes