title: Scoparone Inhibits LPS-Simulated Inflammatory Response by Suppressing IRF3 and ERK in BV-2 Microglial Cells.
abstract: Microglia activation and the release of various inflammatory cytokines are largely related to neurological diseases, including Parkinson's, Alzheimer's, and other brain diseases. The suppression of microglial cells using natural bioactive compounds has become increasingly important for brain therapy owing to the expected beneficial effect of lower toxicity. Scoparone (6,7-dimethoxycoumarin), a major bioactive compound found in various plant parts, including the inner shell of chestnut (Castanea crenata), was evaluated on lipopolysaccharide (LPS)-activated BV-2 microglia cells. The results indicated that scoparone suppresses the LPS-stimulated increase of neuroinflammatory responses and inhibited the pro-inflammatory cytokine production in the BV-2 microglial cells. A mechanistic study showed that scoparone specifically inhibited the LPS-stimulated activation via a major regulation of IRF-3 and a regulation of ERK, whereby the phosphorylation in the BV-2 microglial cells is blocked. These data suggest that scoparone has anti-neuroinflammatory effects in LPS-activated BV-2 microglial cells, and could possibly be used in the development of novel drugs for the prevention and treatment of neuroinflammatory diseases.
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. ejrdus1026@naver.com.
authors.forename: Duk-Yeon
authors.initials: DY
authors.lastname: Cho
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. 997176@kku.ac.kr.
authors.forename: Hyun Myung
authors.initials: HM
authors.lastname: Ko
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. kgfdkr@gmail.com.
authors.forename: Joonsoo
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. Kim.Byungwook@mayo.edu.
authors.forename: Byung-Wook
authors.initials: BW
authors.lastname: Kim
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. aaa003132@naver.com.
authors.forename: Yo-Sep
authors.initials: YS
authors.lastname: Yun
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. pji0819@hanmail.net.
authors.forename: Jeong-In
authors.initials: JI
authors.lastname: Park
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. palanivel@kku.ac.kr.|Nanotechnology Research Center and Department of Applied Life Science, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. palanivel@kku.ac.kr.
authors.forename: Palanivel
authors.initials: P
authors.lastname: Ganesan
authors.affiliation: Department of Cosmeceutical Science, Daegu Haany University, Gyungsan 38610, Korea. jtlee@dhu.ac.kr.
authors.forename: Jin-Tae
authors.initials: JT
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. choidk@kku.ac.kr.|Nanotechnology Research Center and Department of Applied Life Science, College of Biomedical and Health Science, Konkuk University, Chungju 380-701, Korea. choidk@kku.ac.kr.
authors.forename: Dong-Kug
authors.initials: DK
authors.lastname: Choi
mesh_terms: D002460:Cell Line; D003374:Coumarins / Q000494:pharmacology*; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000517:prevention & control*; D050838:Interferon Regulatory Factor-3 / Q000037:antagonists & inhibitors*; D008070:Lipopolysaccharides / Q000633:toxicity*; D017628:Microglia / Q000187:drug effects* / Q000473:pathology; D020928:Mitogen-Activated Protein Kinases / Q000187:drug effects*
chemical_list: D003374:Coumarins; C494232:IRF3 protein, human; D050838:Interferon Regulatory Factor-3; D008070:Lipopolysaccharides; D020928:Mitogen-Activated Protein Kinases; C018145:scoparone
keywords: ERK; IRF-3; microglial cells; scoparone