title: Peptides Derived from Soy and Lupin Protein as Dipeptidyl-Peptidase IV Inhibitors: In Vitro Biochemical Screening and in Silico Molecular Modeling Study.
abstract: Dipeptidyl peptidase IV (DPP-IV) is a new molecular target correlated with the development of type 2 diabetes. Literature describes the identification of some inhibitory peptides from the hydrolysis of different food proteins. This article reports a study on six peptides from soybean and lupin proteins, i.e., Soy 1 (IAVPTGVA), Soy 2 (YVVNPDNDEN), Soy 3 (YVVNPDNNEN), Lup 1 (LTFPGSAED), Lup 2 (LILPKHSDAD), and Lup 3 (GQEQSHQDEGVIVR), which were screened for their capacity to inhibit the activity of DPP-IV, using an in vitro bioassay against human recombinant DPP-IV. Two peptides Soy 1 and Lup 1 resulted to be efficient inhibitors with IC50values equal to 106 and 228 μM, respectively. A molecular docking analysis predicted the key molecular interactions, stabilizing the active peptides within DPP-IV enzyme. Soy and lupin proteins may be sources of DPP-IV inhibitory peptides potentially useful for the prevention of type 2 diabetes.
authors.affiliation: Department of Pharmaceutical Sciences, University of Milan , 20122 Milan, Italy.
authors.forename: Carmen
authors.initials: C
authors.lastname: Lammi
authors.affiliation: Department of Pharmaceutical Sciences, University of Milan , 20122 Milan, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Zanoni
authors.affiliation: Department of Pharmaceutical Sciences, University of Milan , 20122 Milan, Italy.
authors.forename: Anna
authors.identifier: http://orcid.org/0000-0002-0987-3014
authors.initials: A
authors.lastname: Arnoldi
authors.affiliation: Department of Pharmaceutical Sciences, University of Milan , 20122 Milan, Italy.
authors.forename: Giulio
authors.initials: G
authors.lastname: Vistoli
mesh_terms: D000595:Amino Acid Sequence; D003198:Computer Simulation; D018819:Dipeptidyl Peptidase 4 / Q000737:chemistry; D054873:Dipeptidyl-Peptidase IV Inhibitors / Q000737:chemistry*; D006801:Humans; D007700:Kinetics; D031297:Lupinus / Q000737:chemistry*; D062105:Molecular Docking Simulation; D008969:Molecular Sequence Data; D010455:Peptides / Q000737:chemistry*; D010940:Plant Proteins / Q000737:chemistry*; D013025:Glycine max / Q000737:chemistry*
chemical_list: D054873:Dipeptidyl-Peptidase IV Inhibitors; D010455:Peptides; D010940:Plant Proteins; D018819:Dipeptidyl Peptidase 4
keywords: bioactive peptide; dipeptidyl peptidase IV inhibitor; lupin; soy; type 2 diabetes