title: Evidence for ARGONAUTE4-DNA interactions in RNA-directed DNA methylation in plants.
abstract: RNA polymerase V (Pol V) long noncoding RNAs (lncRNAs) have been proposed to guide ARGONAUTE4 (AGO4) to chromatin in RNA-directed DNA methylation (RdDM) in plants. Here, we provide evidence, based on laser UV-assisted zero-length cross-linking, for functionally relevant AGO4-DNA interaction at RdDM targets. We further demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine-tryptophan/tryptophan-glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L. We propose a model in which AGO4-DNA interaction could be responsible for the unique specificities of RdDM.
authors.affiliation: Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Lahmy
authors.affiliation: Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
authors.forename: Dominique
authors.initials: D
authors.lastname: Pontier
authors.affiliation: Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
authors.forename: Natacha
authors.initials: N
authors.lastname: Bies-Etheve
authors.affiliation: Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
authors.forename: Michèle
authors.initials: M
authors.lastname: Laudié
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA.|Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Suhua
authors.initials: S
authors.lastname: Feng
authors.affiliation: Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
authors.forename: Edouard
authors.initials: E
authors.lastname: Jobet
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA.|Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California at Los Angeles, Los Angeles, California 90095, USA.|Department of Pathology, Center for Precision Diagnostics, University of Washington, Seattle, Washington 98195, USA.
authors.forename: Christopher J
authors.initials: CJ
authors.lastname: Hale
authors.affiliation: Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
authors.forename: Richard
authors.initials: R
authors.lastname: Cooke
authors.affiliation: Institute for Advanced Biosciences (IAB), UMR5309, CNRS, U1209, Institut National de la Santé et de la Recherche Médicale (INSERM), Grenoble Alpes University, 38000 Grenoble, France.
authors.forename: Mohamed-Ali
authors.initials: MA
authors.lastname: Hakimi
authors.affiliation: Laboratoire de Biologie et Modélisation de la Cellule (LBMC), UMR 5239, CNRS/École Normale Supérieure de Lyon (ENSL)/Université Claude Bernard Lyon 1 (UCBL), 69007 Lyon, France.|Institut NeuroMyogène (INMG), UMR 5310, CNRS/UCBL/ENSL, 69007 Lyon, France.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Angelov
authors.affiliation: Department of Molecular, Cell, and Developmental Biology, University of California at Los Angeles, Los Angeles, California 90095, USA.|Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California at Los Angeles, Los Angeles, California 90095, USA.|Howard Hughes Medical Institute, University of California at Los Angeles, Los Angeles, California 90095, USA.
authors.forename: Steven E
authors.initials: SE
authors.lastname: Jacobsen
authors.affiliation: Laboratoire Génome et Développement des Plantes (LGDP), UMR5096, Centre National de la Recherche Scientifique (CNRS), Université de Perpignan via Domitia (UPVD), 66860 Perpignan, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Lagrange
mesh_terms: D020816:Amino Acid Motifs / Q000235:genetics; D029681:Arabidopsis Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D060565:Argonaute Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D002843:Chromatin / Q000378:metabolism; D019175:DNA Methylation / Q000235:genetics; D018744:DNA, Plant / Q000378:metabolism*; D012321:DNA-Directed RNA Polymerases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D020868:Gene Silencing; D008954:Models, Biological; D034622:RNA Interference; D018749:RNA, Plant / Q000378:metabolism*; D014158:Transcription, Genetic / Q000235:genetics
chemical_list: C471209:AGO4 protein, Arabidopsis; D029681:Arabidopsis Proteins; D060565:Argonaute Proteins; D002843:Chromatin; D018744:DNA, Plant; D018749:RNA, Plant; C538841:RNA polymerase V, Arabidopsis; D012321:DNA-Directed RNA Polymerases
keywords: Argonaute; DNA methylation; RdDM