title: Comparative characterization of hepatitis B virus surface antigen derived from different hepatitis B virus genotypes.
abstract: For human hepatitis B virus eight distinct and two candidate genotypes are described. These genotypes differ with respect to geographic distribution, molecular virology and virus-associated pathogenesis. Comparative analysis of HBV genotypes revealed, with exception of HBV/G that shows impaired HBsAg release, that no fundamental disparities between genotypes exist regarding glycosylation, subcellular distribution, release of HBsAg and formation of subviral particles. However, there are distinctions regarding the proportion of L to M to S HBs proteins detected intra- and extracellularly for different genotypes. 2D electrophoresis revealed different posttranslational modification patterns for LHBs. In light of the relevance of HBsAg as diagnostic marker, detectability of purified recombinant HBsAg of various genotypes by HBsAg-specific detection systems licensed in Europe was investigated, showing similar sensitivities for genotypes included in this analysis. These data indicate that recombinant HBsAg reproducibly purified following a defined protocol might be used as an alternative to reference materials currently established.
authors.affiliation: Paul-Ehrlich-Institut, Department of Virology, Langen, Germany.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hassemer
authors.affiliation: Paul-Ehrlich-Institut, Department of Virology, Langen, Germany.
authors.forename: Malin
authors.initials: M
authors.lastname: Finkernagel
authors.affiliation: University Hospital Frankfurt, Frankfurt/Main, Germany; Goethe University Frankfurt, Frankfurt/Main, Germany.
authors.forename: Kai-Henrik
authors.initials: KH
authors.lastname: Peiffer
authors.affiliation: Justus Liebig University, Institute of Medical Virology, Gießen, Germany; German Center for Infection Research (DZIF), Gießen-Marburg-Langen, Germany.
authors.forename: Dieter
authors.initials: D
authors.lastname: Glebe
authors.affiliation: Paul-Ehrlich-Institut, Department of Virology, Langen, Germany.
authors.forename: Sami
authors.initials: S
authors.lastname: Akhras
authors.affiliation: Paul-Ehrlich-Institut, Department of Allergology, Langen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Reuter
authors.affiliation: Paul-Ehrlich-Institut, in-vitro-diagnostics, Langen, Germany.
authors.forename: Heinrich
authors.initials: H
authors.lastname: Scheiblauer
authors.affiliation: University Hospital Frankfurt, Frankfurt/Main, Germany; Goethe University Frankfurt, Frankfurt/Main, Germany.
authors.forename: Lisa
authors.initials: L
authors.lastname: Sommer
authors.affiliation: Paul-Ehrlich-Institut, Department of Virology, Langen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Chudy
authors.affiliation: Paul-Ehrlich-Institut, Department of Virology, Langen, Germany.
authors.forename: C Micha
authors.initials: CM
authors.lastname: Nübling
authors.affiliation: Paul-Ehrlich-Institut, Department of Virology, Langen, Germany; German Center for Infection Research (DZIF), Gießen-Marburg-Langen, Germany. Electronic address: eberhard.hildt@pei.de.
authors.forename: Eberhard
authors.initials: E
authors.lastname: Hildt
mesh_terms: D005838:Genotype; D006031:Glycosylation; D006509:Hepatitis B / Q000821:virology*; D006514:Hepatitis B Surface Antigens / Q000235:genetics* / Q000378:metabolism; D006515:Hepatitis B virus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D006801:Humans
chemical_list: D006514:Hepatitis B Surface Antigens
keywords: Genotypes; HBsAg; Hepatitis B virus; Surface protein