title: Increased serum levels of macrophage activation marker sCD163 in Dengue patients.
abstract: Dengue viruses are known to infect and replicate in macrophages. Thus studying the host responsive molecules that are specifically released by macrophages during the course of dengue infection may provide better understanding on dengue immunopathogenesis. Soluble CD163 (sCD163) is a scavenger receptor, highly expressed on macrophages reported to be involved in some viral disease. The participation of sCD163 in dengue is not known.
  
  
  
  The present study aimed to explore the role of sCD163 as a potential biomarker for predicting dengue disease.
  
  
  
  Using a case-control design, 82 dengue subjects consisting of 69 non-severe dengue (NSD) and 13 severe dengue (SD) along with 32 non-dengue other febrile illness (OFI) subjects and 30 healthy subjects were involved in the study. The serum concentration of sCD163 was determined in the study subjects at admission and around defervescence using ELISA. Statistical analysis was done using Mann-Whitney U test andWilcoxon signed rank test.
  
  
  
  The study recorded a significant increase in the sCD163 serum level at defervescence phase specifically among dengue group compared to OFI. sCD163 was also found to be significantly higher in secondary cases compared to primary at both admission and defervescence. Furthermore,a higher level of sCD163 was also observed in SD compared to NSD cases, although no statistical significance was observed CONCLUSION: The study substantiates the role of macrophage activation in dengue pathogenesis and further study is needed to decipher the exact role of sCD163 in the disease pathogenesis and to explore its potential as a marker for the early prediction of dengue severity.
authors.affiliation: Department of Biochemistry, JIPMER, Puducherry, India.
authors.forename: Sarayu Gopal
authors.initials: SG
authors.lastname: S S
authors.affiliation: Central Inter-Disciplinary Research Facility (CIDRF), A Unit of Sri Balaji Educational and Charitable Public Trust, SBVU, Puducherry, India.
authors.forename: Agieshkumar B
authors.initials: AB
authors.lastname: Pillai
authors.affiliation: Department of Biochemistry, JIPMER, Puducherry, India.
authors.forename: Vijayakumar Shettikothanuru
authors.initials: VS
authors.lastname: Ramachandrappa
authors.affiliation: Department of Medicine, JIPMER, Puducherry, India.
authors.forename: Kadhiravan
authors.initials: K
authors.lastname: T
authors.affiliation: Department of Microbiology, JIPMER, Puducherry, India.
authors.forename: Rahul
authors.initials: R
authors.lastname: Dhodapkar
authors.affiliation: Dept of Bio engineering, National University of Singapore, 119077, Singapore.
authors.forename: James
authors.initials: J
authors.lastname: Kah
authors.affiliation: Department of Biochemistry, JIPMER, Pondicherry, 605 006, India. Electronic address: soundy27@gmail.com.
authors.forename: Soundravally
authors.initials: S
authors.lastname: Rajendiran
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood*; D015214:Antigens, Differentiation, Myelomonocytic / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D003715:Dengue / Q000473:pathology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D008262:Macrophage Activation; D008297:Male; D011956:Receptors, Cell Surface / Q000097:blood*; D044967:Serum / Q000737:chemistry*; D055815:Young Adult; D000098902:CD163 Antigen
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D015415:Biomarkers; D000098902:CD163 Antigen; D011956:Receptors, Cell Surface
keywords: Macrophage activation; sCD163