title: Cytological behaviour of floral organs and in silico characterization of differentially expressed transcript-derived fragments associated with 'floral bud distortion' in soybean.
abstract: An attempt was made to understand the 'floral bud distortion' (FBD), an unexplored disorder prevailing in soybean. Cytological behaviour of floral reproductive organs and in silico characterization of differentially expressed transcript-derived fragments (TDFs) in symptomatic and asymptomatic soybean plants were carried out. Pollens in asymptomatic plants do not have defects in number, size, shape and function. However, in symptomatic plant, pollens were found nonviable, abnormal in shape and with reduced germination ability. Here, we employed a computational approach, exploring invaluable resources. The tissue-specific transcript profile of symptomatic and asymptomatic sources was compared to determine differentially expressed TDFs associated with FBD to improve its basic understanding. A total of 60 decamer primers produced 197 scorable amplicons, ranged 162-1130 bp, of which 171 were monomorphic and 26 were differentially regulated. Reproducible TDFs were sequenced and characterized for their homology analysis, annotation, protein-protein interaction, subcellular localization and their physical mapping. Homology-based annotation of TDFs in soybean revealed presence of two characterized and seven uncharacterized hits. Annotation of characterized sequences showed presence of genes, namely auxin response factor 9 (ARF9) and forkhead-associated (FHA) domain, which are directly involved in plant development through various pathways, such as hormonal regulation, plant morphology, embryogenesis and DNA repair.
authors.affiliation: Biotechnology Centre, Post Graduate Institute, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola 444 104, India. jpraveen26@yahoo.co.in.
authors.forename: Prashant B
authors.initials: PB
authors.lastname: Kale
authors.forename: Pravin V
authors.initials: PV
authors.lastname: Jadhav
authors.forename: Rachana S
authors.initials: RS
authors.lastname: Wakekar
authors.forename: M P
authors.initials: MP
authors.lastname: Moharil
authors.forename: A G
authors.initials: AG
authors.lastname: Deshmukh
authors.forename: M S
authors.initials: MS
authors.lastname: Dudhare
authors.forename: R S
authors.initials: RS
authors.lastname: Nandanwar
authors.forename: S S
authors.initials: SS
authors.lastname: Mane
authors.forename: J G
authors.initials: JG
authors.lastname: Manjaya
authors.forename: R G
authors.initials: RG
authors.lastname: Dani
mesh_terms: D032461:Chromosomes, Plant; D019295:Computational Biology / Q000379:methods; D035264:Flowers / Q000235:genetics*; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D063990:Gene Ontology; D053263:Gene Regulatory Networks; D017343:Genes, Plant; D058977:Molecular Sequence Annotation; D020161:Physical Chromosome Mapping; D011058:Pollen / Q000033:anatomy & histology / Q000166:cytology / Q000648:ultrastructure; D025941:Protein Interaction Mapping; D060066:Protein Interaction Maps; D021381:Protein Transport; D020543:Proteome; D040901:Proteomics / Q000379:methods; D019655:Quantitative Trait, Heritable; D017422:Sequence Analysis, DNA; D013025:Glycine max / Q000166:cytology / Q000235:genetics* / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D020543:Proteome