title: Quantitative Determination of Cannabinoids in Cannabis and Cannabis Products Using Ultra-High-Performance Supercritical Fluid Chromatography and Diode Array/Mass Spectrometric Detection.
abstract: Ultra-high-performance supercritical fluid chromatography (UHPSFC) is an efficient analytical technique and has not been fully employed for the analysis of cannabis. Here, a novel method was developed for the analysis of 30 cannabis plant extracts and preparations using UHPSFC/PDA-MS. Nine of the most abundant cannabinoids, viz. CBD, ∆8-THC, THCV, ∆9-THC, CBN, CBG, THCA-A, CBDA, and CBGA, were quantitatively determined (RSDs < 6.9%). Unlike GC methods, no derivatization or decarboxylation was required prior to UHPSFC analysis. The UHPSFC chromatographic separation of cannabinoids displayed an inverse elution order compared to UHPLC. Combining with PDA-MS, this orthogonality is valuable for discrimination of cannabinoids in complex matrices. The developed method was validated, and the quantification results were compared with a standard UHPLC method. The RSDs of these two methods were within ±13.0%. Finally, chemometric analysis including principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA) were used to differentiate between cannabis samples.
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
authors.forename: Mei
authors.initials: M
authors.lastname: Wang
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Wang
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
authors.forename: Bharathi
authors.initials: B
authors.lastname: Avula
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.|Department of Pharmacognosy, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt.
authors.forename: Mohamed M
authors.initials: MM
authors.lastname: Radwan
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.|Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia, Egypt.
authors.forename: Amira S
authors.initials: AS
authors.lastname: Wanas
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
authors.forename: Zlatko
authors.initials: Z
authors.lastname: Mehmedic
authors.affiliation: Waters Corporation, Milford, MA, 01757, USA.
authors.forename: John
authors.initials: J
authors.lastname: van Antwerp
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.|Department of Pharmaceutics and Drug Delivery, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.
authors.forename: Mahmoud A
authors.initials: MA
authors.lastname: ElSohly
authors.affiliation: National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.|Division of Pharmacognosy, Department of BioMolecular Science, School of Pharmacy, University of Mississippi, Oxford, MS, 38677, USA.|Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
mesh_terms: D002186:Cannabinoids / Q000032:analysis* / Q000737:chemistry; D002188:Cannabis / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid; D006801:Humans; D016018:Least-Squares Analysis; D057230:Limit of Detection; D013058:Mass Spectrometry; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry; D025341:Principal Component Analysis
chemical_list: D002186:Cannabinoids; D010936:Plant Extracts
keywords: Cannabis sativa L.; UHPSFC/PDA-MS; cannabinoids; chemometric analysis; forensic science; quantification and method validation