title: Cellulase immobilization on magnetic nanoparticles encapsulated in polymer nanospheres.
abstract: Immobilization of cellulases on magnetic nanoparticles, especially magnetite nanoparticles, has been the main approach studied to make this enzyme, economically and industrially, more attractive. However, magnetite nanoparticles tend to agglomerate, are very reactive and easily oxidized in air, which has strong impact on their useful life. Thus, it is very important to provide proper surface coating to avoid the mentioned problems. This study aimed to investigate the immobilization of cellulase on magnetic nanoparticles encapsulated in polymeric nanospheres. The support was characterized in terms of morphology, average diameter, magnetic behavior and thermal decomposition analyses. The polymer nanospheres containing encapsulated magnetic nanoparticles showed superparamagnetic behavior and intensity average diameter about 150 nm. Immobilized cellulase exhibited broader temperature stability than in the free form and great reusability capacity, 69% of the initial enzyme activity was maintained after eight cycles of use. The magnetic support showed potential for cellulase immobilization and allowed fast and easy biocatalyst recovery through a single magnet.
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Janaina S
authors.initials: JS
authors.lastname: Lima
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Pedro H H
authors.initials: PH
authors.lastname: Araújo
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Claudia
authors.initials: C
authors.lastname: Sayer
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil.
authors.forename: Antonio A U
authors.initials: AA
authors.lastname: Souza
authors.affiliation: Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Alexandre C
authors.initials: AC
authors.lastname: Viegas
authors.affiliation: Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina (UFSC), Florianópolis, SC, 88040-900, Brazil. debora.oliveira@ufsc.br.
authors.forename: Débora
authors.initials: D
authors.lastname: de Oliveira
mesh_terms: D002480:Cellulase / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D060526:Magnetic Fields; D053758:Nanoparticles / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D019904:Polymethyl Methacrylate; D002480:Cellulase
keywords: Cellulase; Enzymes immobilization; Immobilized cellulase; Magnetic nanoparticles