title: Chemical profiling of the tuber of Stephania cambodica Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD.
abstract: A new aporphine glycoside (1), named 'angkorwatine', and eight known alkaloids: oblongine (2), stepharine (3), asimilobine-β-d-glucopyranoside (4), isocorydine (5), tetrahydropalmatine (THP) (6), jatrorrhizine (7), palmatine (PAL) (8), and roemerine (ROE) (9) were simultaneously isolated from the tuber of Stephania cambodica. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of S. cambodica. In addition to good selectivity and linearity (r2> 0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39-4.18 μg/mL, 2.01-30.72 μg/mL, and 4.29-64.42 μg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of S. cambodica and to regulate the usage of this plant in traditional medicine.
authors.affiliation: a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.|b Research Unit , University of Health Sciences , Phnom Penh , Cambodia.|c Muséum national d'Histoire naturelle, Institut de Systématique, Évolution, Biodiversité, UMR 7205 ISYEB MNHN/CNRS/UPMC/EPHE , Sorbonne Université , Paris , France.
authors.forename: Chhavarath
authors.initials: C
authors.lastname: Dary
authors.affiliation: a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.
authors.forename: Sok-Siya
authors.initials: SS
authors.lastname: Bun
authors.affiliation: d Spectropole, Service 511, Aix Marseille Univ , Marseille , France.
authors.forename: Gaëtan
authors.initials: G
authors.lastname: Herbette
authors.affiliation: a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.
authors.forename: Fathi
authors.initials: F
authors.lastname: Mabrouki
authors.affiliation: e Laboratory of Pharmacokinetics and Toxicokinetics, Faculty of Pharmacy , Aix Marseille Univ , Marseille , France.
authors.forename: Hot
authors.initials: H
authors.lastname: Bun
authors.affiliation: f Laboratory of Phytochemistry, Faculty of Pharmacy , University of Health Sciences , Phnom Penh , Cambodia.
authors.forename: Sothea
authors.initials: S
authors.lastname: Kim
authors.affiliation: c Muséum national d'Histoire naturelle, Institut de Systématique, Évolution, Biodiversité, UMR 7205 ISYEB MNHN/CNRS/UPMC/EPHE , Sorbonne Université , Paris , France.
authors.forename: Florian
authors.initials: F
authors.lastname: Jabbour
authors.affiliation: c Muséum national d'Histoire naturelle, Institut de Systématique, Évolution, Biodiversité, UMR 7205 ISYEB MNHN/CNRS/UPMC/EPHE , Sorbonne Université , Paris , France.
authors.forename: Sovanmoly
authors.initials: S
authors.lastname: Hul
authors.affiliation: a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.
authors.forename: Béatrice
authors.initials: B
authors.lastname: Baghdikian
authors.affiliation: a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.
authors.forename: Evelyne
authors.initials: E
authors.lastname: Ollivier
mesh_terms: D000470:Alkaloids / Q000737:chemistry; D001060:Aporphines; D001600:Berberine Alkaloids; D002851:Chromatography, High Pressure Liquid; D007546:Isoquinolines; D009682:Magnetic Resonance Spectroscopy; D029583:Menispermaceae / Q000737:chemistry*; D035281:Plant Tubers / Q000737:chemistry*; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization; D031607:Stephania / Q000737:chemistry*
chemical_list: D000470:Alkaloids; D001060:Aporphines; D001600:Berberine Alkaloids; D007546:Isoquinolines; C081279:aporphine; C009320:stepharine; C030168:isocorydine; C014215:tetrahydropalmatine; C030169:roemerine; C081049:oblongine; C054614:asimilobine; C005413:palmatine
keywords: Accuracy profile; alkaloid; method validation; palmatine; quantification; roemerine; tetrahydropalmatine