title: Marker-assisted improvement of the elite restorer line of rice, RPHR-1005 for resistance against bacterial blight and blast diseases.
abstract: This study was carried out to improve the RPHR-1005, a stable restorer line of the popular medium slender grain type rice hybrid, DRRH-3 for bacterial blight (BB) and blast resistance through marker-assisted backcross breeding (MABB). Two major BB resistance genes, Xa21 and Xa33 and a major blast resistance gene, Pi2 were transferred to RPHR-1005 as two individual crosses. Foreground selection for Xa21, Xa33, Pi2, Rf3 and Rf4 was done by using gene-specific functional markers, while 59 simple sequence repeat (SSR) markers polymorphic between the donors and recipient parents were used to select the best plant possessing target resistance genes at each backcross generation. Backcrossing was continued till BC2F2and a promising homozygous backcross derived line possessing Xa21+ Pi2 and another possessing Xa33 were intercrossed to stack the target resistance genes into the genetic background of RPHR-1005. At ICF4, 10 promising lines possessing three resistance genes in homozygous condition along with fine-grain type, complete fertility restoration, better panicle exertion and taller plant type (compared to RPHR-1005) were identified.
authors.affiliation: ICAR-Indian Institute of Rice Research (IIRR), Rajendranagar, Hyderabad 5000 30, India. rms_28@rediffmail.com.
authors.forename: V Abhilash
authors.initials: VA
authors.lastname: Kumar
authors.forename: C H
authors.initials: CH
authors.lastname: Balachiranjeevi
authors.forename: S Bhaskar
authors.initials: SB
authors.lastname: Naik
authors.forename: R
authors.initials: R
authors.lastname: Rambabu
authors.forename: G
authors.initials: G
authors.lastname: Rekha
authors.forename: G
authors.initials: G
authors.lastname: Harika
authors.forename: S K
authors.initials: SK
authors.lastname: Hajira
authors.forename: K
authors.initials: K
authors.lastname: Pranathi
authors.forename: S
authors.initials: S
authors.lastname: Vijay
authors.forename: M
authors.initials: M
authors.lastname: Anila
authors.forename: H K
authors.initials: HK
authors.lastname: Mahadevaswamy
authors.forename: M
authors.initials: M
authors.lastname: Kousik
authors.forename: A
authors.initials: A
authors.lastname: Yugander
authors.forename: J
authors.initials: J
authors.lastname: Aruna
authors.forename: A S
authors.initials: AS
authors.lastname: Hari Prasad
authors.forename: M S
authors.initials: MS
authors.lastname: Madhav
authors.forename: G S
authors.initials: GS
authors.lastname: Laha
authors.forename: S M
authors.initials: SM
authors.lastname: Balachandran
authors.forename: M S
authors.initials: MS
authors.lastname: Prasad
authors.forename: V Ravindra
authors.initials: VR
authors.lastname: Babu
authors.forename: R M
authors.initials: RM
authors.lastname: Sundaram
mesh_terms: D001419:Bacteria; D001947:Breeding; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D005819:Genetic Markers; D005838:Genotype; D006824:Hybridization, Genetic; D018895:Microsatellite Repeats; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology*; D012641:Selection, Genetic
chemical_list: D005819:Genetic Markers