title: L-Carnitine in rooster semen cryopreservation: Flow cytometric, biochemical and motion findings for frozen-thawed sperm.
abstract: Rooster semen cryopreservation is not efficient for artificial insemination in breeder flocks. L-Carnitine (LC) has been evaluated for effectiveness in cryopreservation media on the characteristics of rooster sperm after freeze-thawing. Motility characteristics, membrane functionality, abnormal morphology, apoptotic like changes, mitochondria activity and lipid peroxidation of rooster sperms were assessed after freeze-thawing with different concentrations of LC in Beltsville medium. Semen samples were collected from 12 roosters, twice a week, and diluted in the extenders that contained different concentrations of LC. Supplementation of Beltsevile with 1 and 2 mM LC was found to result in higher total motility (68.2± 1.7% and 69.1± 1.7%, respectively), progressive motility (28.4± 1.6%, 29.8± 1.6%), membrane functionality (76.2± 1.9% and 75.9± 1.9%), viability (58.2 ± 1.1%, 59.1 ± 1.1%) and lower significant of lipid peroxidation (2.53 ± 0.08 nmol/ml, 2.49 ± 0.08 nmol/ml) compared to control group containing no LC. Lower motility, progressive motility, and viability were observed in frozen-thawed sperm in extender containing 8 mM LC (35.8± 1.7%, 9.6± 1.2% and 27.1 ± 1.2%, respectively) compared to control. Morphology and mitochondrial activity were not affected by different concentrations of LC. Our results showed that supplementation of Beltsville extender with 1 and 2 mM LC significantly improved the quality of rooster sperm quality after freeze-thawing.
authors.affiliation: Department of Animal Science, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran. Electronic address: amir1356fattah@qodsiau.ac.ir.
authors.forename: A
authors.initials: A
authors.lastname: Fattah
authors.affiliation: Department of Poultry Science, Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran. Electronic address: m.sharafi@modares.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Sharafi
authors.affiliation: Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Masoudi
authors.affiliation: Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Shahverdi
authors.affiliation: Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: V
authors.initials: V
authors.lastname: Esmaeili
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
authors.forename: A
authors.initials: A
authors.lastname: Najafi
mesh_terms: D000818:Animals; D002331:Carnitine / Q000494:pharmacology*; D002462:Cell Membrane / Q000502:physiology*; D002645:Chickens; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005434:Flow Cytometry; D005615:Freezing; D007315:Insemination, Artificial / Q000379:methods; D015227:Lipid Peroxidation / Q000502:physiology; D008297:Male; D008928:Mitochondria / Q000502:physiology; D012661:Semen / Q000378:metabolism; D055101:Semen Analysis / Q000662:veterinary*; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology
chemical_list: D003451:Cryoprotective Agents; D002331:Carnitine
keywords: Apoptosis; Fowl; Mitochondria activity; Sperm freezing