title: Effect of calcitonin on cultured schwann cells.
abstract: BACKGROUND
  After nerve injury, calcium concentrations in intranerve fibers quickly increase. We have shown that functional recovery of injured nerves correlates with calcium absorption. A slight increase in calcium reduces the number of Schwann cells present. Calcitonin therapy greatly improves regeneration by accelerating calcium absorption. We examined the effect of adding calcitonin to higher concentration calcium media on cultured Schwann cells.
  
  
  METHODS
  The cells, isolated from intact sciatic nerves, were cultured with normal or higher concentration calcium media with or without calcitonin. Schwann cells were incubated with anti-S-100, goat-anti-mouse, and propidium iodide and then viewed through fluorescent light and phase-contrast microscopy for observation and analysis.
  
  
  RESULTS
  The cells in each calcitonin-containing medium showed many Schwann cells, however, the cells in the higher concentration calcium media showed fewer and more defective Schwann cells.
  
  
  CONCLUSIONS
  These results show that calcitonin protects against the harmful effects of excessive calcium encountered in peripheral nerve injury. Muscle Nerve 56: 768-772, 2017.
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: Ji-Geng
authors.initials: JG
authors.lastname: Yan
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: Lin-Ling
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Agresti
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: Fengyi
authors.initials: F
authors.lastname: Shen
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: Hani S
authors.initials: HS
authors.lastname: Matloub
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Hand Surgery, Huashan Hospital, Fudan University and Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China.
authors.forename: Jifeng
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Hand Surgery, Huashan Hospital, Fudan University and Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai, China.
authors.forename: Yu-Dong
authors.initials: YD
authors.lastname: Gu
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: John A
authors.initials: JA
authors.lastname: Logiudice
authors.affiliation: Department of Plastic Surgery, Medical College of Wisconsin, 8700 Watertown Plank Road, Milwaukee, Wisconsin, 53226, USA.
authors.forename: Robert
authors.initials: R
authors.lastname: Havlik
mesh_terms: D000818:Animals; D050071:Bone Density Conservation Agents / Q000494:pharmacology; D002116:Calcitonin / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism* / Q000494:pharmacology; D002478:Cells, Cultured; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D050071:Bone Density Conservation Agents; D002116:Calcitonin; D002118:Calcium
keywords: Schwann cells; calcitonin; calcium; nerve injury; nerve regeneration