title: Fabrication and characterization of platelet-rich plasma scaffolds for tissue engineering applications.
abstract: Platelet-Rich Plasma (PRP), as a rich source of growth factor, can form a fibrin gel that recapitulates the extracellular matrix of the tissues. The aim of this study was to evaluate the effects of different concentrations of CaCl2on the PRP scaffold structure which in turn could change the cell's behavior. PRP was mixed with 2.5, 5 and 10% (w/v) CaCl2. Then, the tensile strength, biodegradability and water content of the scaffolds were evaluated. We also performed immunostaining for assessment of the actin stress fiber orientation and SEM for detecting the cell phenotype and physical properties of the fibers. Cell viability, attachment and migration were also evaluated. The highest cell attachment and short term proliferation rate was observed on the scaffolds with 2.5% CaCl2. The cells cultured on the scaffold with higher CaCl2concentration had fusiform phenotype with few cell processes and parallel arrangement of stress fibers while those cultured on the other scaffolds were fibroblast-like with more processes and net-like stress fibers. The scaffolds with 10% CaCl2demonstrated the highest osmolarity (358.75±4.99mOsmole), fiber thickness (302.1±54.3nm), pore size (332.1±118.9nm2) and the longest clotting time (12.2±0.776min) compared with the other scaffolds. Water content, branching angle, porosity, orientation and tensile strength did not change by gelation with different CaCl2concentrations. In conclusion, the cell shape, viability and proliferation were modified by culturing on the PRP scaffolds prepared with various concentrations of CaCl2, and as a result, the scaffolds showed different physical and biological properties.
authors.affiliation: Tissue engineering Lab, Anatomy department, Medical School, Shiraz University of Medical Sciences, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Sadeghi-Ataabadi
authors.affiliation: Recombinant protein lab, Department of Biochemistry, Medical School, Shiraz University of Medical Sciences, Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Mostafavi-Pour
authors.affiliation: Tissue engineering Lab, Anatomy department, Medical School, Shiraz University of Medical Sciences, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Vojdani
authors.affiliation: Tissue engineering Lab, Anatomy department, Medical School, Shiraz University of Medical Sciences, Iran.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Sani
authors.affiliation: Tissue Engineering Department, National Institute of Genetic Engineering and Biotechnoloy, Iran; Tissue engineering Lab, Anatomy department, Medical School, Shiraz University of Medical Sciences, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Latifi
authors.affiliation: Tissue engineering Lab, Anatomy department, Medical School, Shiraz University of Medical Sciences, Iran. Electronic address: talaeit@sums.ac.ir.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Talaei-Khozani
mesh_terms: D002122:Calcium Chloride / Q000737:chemistry; D002470:Cell Survival; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D053657:Platelet-Rich Plasma / Q000737:chemistry*; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D002122:Calcium Chloride
keywords: CaCl(2); Mesenchymal stem cell; Platelet rich plasma; Tissue engineering