title: Flight of Rhyzopertha dominica (Coleoptera: Bostrichidae)-a Spatio-Temporal Analysis With Pheromone Trapping and Population Genetics.
abstract: The flight of the lesser grain borer, Rhyzopertha dominica (F.), near grain storages and at distances from them, was investigated to assess the potential of these beetles to infest grain and spread insecticide resistance genes. We caught R. dominica in pheromone-baited flight traps (and blank controls) set at storages, in fields away from storages, and in native vegetation across a 12-mo period. A functional set of highly polymorphic microsatellite markers was developed, enabling population genetic analyses on the trapped beetles. Pheromone-baited traps caught just as many R. dominica adults at least 1 km from grain storages as were caught adjacent to grain storages. Samples of beetles caught were genetically homogeneous across the study area (over 7,000 km2) in South Queensland, Australia. However, a change in genetic structure was detected at one bulk storage site. Subsequent analysis detected a heterozygous excess, which indicated a population bottleneck. Only a few beetles were caught during the winter months of June and July. To assess the mating status and potential fecundity of dispersing R. dominica females, we captured beetles as they left grain storages and quantified offspring production and life span in the laboratory. Nearly all (95%) of these dispersing females had mated and these produced an average of 242 offspring. We demonstrated that R. dominica populations in the study area display a high degree of connectivity and this is a result of the active dispersal of mated individuals of high potential fecundity.
authors.affiliation: Department of Agriculture and Fisheries, Agri-Science Queensland, EcoSciences Precinct, GPO Box 267, Brisbane, Qld 4001, Australia (Andrew.Ridley@daf.qld.gov.au; greg.daglish@daf.qld.gov.au) Andrew.Ridley@daf.qld.gov.au.|Cooperative Research Centre for National Plant Biosecurity, LPO Box 5012, Bruce, ACT 2617, Australia (Raghu.Sathyamurthy@csiro.au).
authors.forename: A W
authors.initials: AW
authors.lastname: Ridley
authors.affiliation: School of Biological Sciences, The University of Queensland, Brisbane, Qld 4072, Australia (j.hereward@uq.edu.au; g.mcculloch1@uq.edu.au; g.walter@uq.edu.au).
authors.forename: J P
authors.initials: JP
authors.lastname: Hereward
authors.affiliation: Department of Agriculture and Fisheries, Agri-Science Queensland, EcoSciences Precinct, GPO Box 267, Brisbane, Qld 4001, Australia (Andrew.Ridley@daf.qld.gov.au; greg.daglish@daf.qld.gov.au).|Cooperative Research Centre for National Plant Biosecurity, LPO Box 5012, Bruce, ACT 2617, Australia (Raghu.Sathyamurthy@csiro.au).
authors.forename: G J
authors.initials: GJ
authors.lastname: Daglish
authors.affiliation: Cooperative Research Centre for National Plant Biosecurity, LPO Box 5012, Bruce, ACT 2617, Australia (Raghu.Sathyamurthy@csiro.au).|CSIRO, EcoSciencesPrecinct, GPO Box 2583, Brisbane, Qld 4001, Australia.
authors.forename: S
authors.initials: S
authors.lastname: Raghu
authors.affiliation: School of Biological Sciences, The University of Queensland, Brisbane, Qld 4072, Australia (j.hereward@uq.edu.au; g.mcculloch1@uq.edu.au; g.walter@uq.edu.au).
authors.forename: G A
authors.initials: GA
authors.lastname: McCulloch
authors.affiliation: School of Biological Sciences, The University of Queensland, Brisbane, Qld 4072, Australia (j.hereward@uq.edu.au; g.mcculloch1@uq.edu.au; g.walter@uq.edu.au).
authors.forename: G H
authors.initials: GH
authors.lastname: Walter
mesh_terms: D063147:Animal Distribution; D000818:Animals; D001517:Coleoptera / Q000235:genetics / Q000502:physiology*; D005260:Female; D005298:Fertility; D014644:Genetic Variation; D007300:Insect Control; D008297:Male; D010675:Pheromones / Q000494:pharmacology; D011793:Queensland; D012726:Sexual Behavior, Animal; D062211:Spatio-Temporal Analysis
chemical_list: D010675:Pheromones; C010411:insect attractants
keywords: dispersal; lesser grain borer; microsatellite