title: Tripterygium wilfordii polyglycosidium ameliorates pouchitis induced by dextran sulfate sodium in rats.
abstract: BACKGROUND
  The aim of this study was to investigate the therapeutic effects of Tripterygium wilfordii polyglycosidium (TWP) to rats with dextran sulfate sodium (DSS)-induced pouchitis and its possible mechanism.
  
  
  METHODS
  Sprague-Dawley rats underwent surgery of ileal pouch anal anastomosis (IPAA) and pouchitis was induced by DSS. Rats were randomly divided into no intervention (NI), normal saline (NS) and TWP groups. Rats were lavaged with normal saline (3ml/day in NS group) or TWP (12mg/kg/day in TWP group) for 7days. General conditions of animals and histopathological examinations were evaluated. Interleukin (IL)-1β, IL-6, IL-10, and tumor necrosis factor (TNF)-α mRNA expression was measured. Levels of occludin and Zo-1 proteins were measured by immunohistochemistry. In addition, ALT and AST were assessed.
  
  
  RESULTS
  TWP significantly attenuated the symptoms of pouchitis characterized by body weight loss, diarrhea, and bloody stool. Furthermore, TWP diminished histological damage compared with other groups. There was a significant reduction in levels of IL-1β, IL-6, and TNF-α, as well as an increase in IL-10 in the TWP group. The expression of tight junction proteins occludin and Zo-1 were increased in the TWP group. There were no statistical differences in serum ALT and AST among the three groups.
  
  
  CONCLUSIONS
  TWP significantly ameliorated pouchitis and inhibited the production of IL-1β, IL-6, and TNF-α as well as increased the levels of IL-10, occludin, and Zo-1 protein in rats. These findings suggest TWP might be a potential therapeutic agent for patients with pouchitis.
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Senyang
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Li
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China.
authors.forename: Litian
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital, Tianjin, China. Electronic address: landmark1503@sina.com.
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D004224:Diterpenes / Q000627:therapeutic use*; D004852:Epoxy Compounds / Q000627:therapeutic use; D006801:Humans; D007082:Ileum / Q000601:surgery; D007413:Intestinal Mucosa / Q000187:drug effects* / Q000473:pathology; D008297:Male; D062793:Occludin / Q000378:metabolism; D010616:Phenanthrenes / Q000627:therapeutic use*; D019449:Pouchitis / Q000139:chemically induced / Q000188:drug therapy*; D051381:Rats; D017207:Rats, Sprague-Dawley; D029757:Tripterygium / Q000276:immunology*; D062826:Zonula Occludens-1 Protein / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D004224:Diterpenes; D004852:Epoxy Compounds; D062793:Occludin; D010616:Phenanthrenes; C569043:Tjp1 protein, mouse; D062826:Zonula Occludens-1 Protein; C001899:triptolide; D016264:Dextran Sulfate
keywords: Dextran sulfate sodium; Ileal pouch anal anastomosis; Postoperative complication; Pouchitis; Tripterygium wilfordii