title: Genomic Identification and Comparative Expansion Analysis of the Non-Specific Lipid Transfer Protein Gene Family in Gossypium.
abstract: Plant non-specific lipid transfer proteins (nsLTPs) are involved in many biological processes. In this study, 51, 47 and 91 nsLTPs were identified in Gossypium arboreum, G. raimondii and their descendant allotetraploid G. hirsutum, respectively. All the nsLTPs were phylogenetically divided into 8 distinct subfamilies. Besides, the recent duplication, which is considered cotton-specific whole genome duplication, may have led to nsLTP expansion in Gossypium. Both tandem and segmental duplication contributed to nsLTP expansion in G. arboreum and G. hirsutum, while tandem duplication was the dominant pattern in G. raimondii. Additionally, the interspecific orthologous gene pairs in Gossypium were identified. Some GaLTPs and GrLTPs lost their orthologs in the Atand Dtsubgenomes, respectively, of G. hirsutum. The distribution of these GrLTPs and GaLTPs within each subfamily was complementary, suggesting that the loss and retention of nsLTPs in G. hirsutum might not be random. Moreover, the nsLTPs in the Atand Dtsubgenomes might have evolved symmetrically. Furthermore, both intraspecific and interspecific orthologous genes showed considerable expression variation, suggesting that their functions were strongly differentiated. Our results lay an important foundation for expansion and evolutionary analysis of the nsLTP family in Gossypium, and advance nsLTP studies in other plants, especially polyploid plants.
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.|College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Fan
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Fanglu
authors.initials: F
authors.lastname: Ma
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Erkui
authors.initials: E
authors.lastname: Yue
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.|Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
authors.forename: Noreen
authors.initials: N
authors.lastname: Bibi
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Shen
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Md Mosfeq-Ul
authors.initials: MM
authors.lastname: Hasan
authors.affiliation: Institute of Crop Science, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, People's Republic of China.
authors.forename: Xuede
authors.initials: X
authors.lastname: Wang
mesh_terms: D052179:Antigens, Plant / Q000235:genetics*; D002352:Carrier Proteins / Q000235:genetics*; D020440:Gene Duplication / Q000502:physiology*; D018745:Genome, Plant / Q000502:physiology*; D003368:Gossypium / Q000235:genetics*; D005810:Multigene Family / Q000502:physiology*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*
chemical_list: D052179:Antigens, Plant; D002352:Carrier Proteins; D010940:Plant Proteins; C511920:lipid transfer proteins, plant