title: Profiling of sperm gene transcripts in crossbred (Bos taurus x Bos indicus) bulls.
abstract: Crossbred cattle in some sectors of the world have a significant role in enhancing milk production thereby enhancing the per capita milk availability as a human food source. However, there are certain constraints associated with crossbred animals, such as disease susceptibility, increased reproductive problems, repeat breeding and poor seminal quality. The semen of crossbred bulls has a poor freezing capacity, increased cryo-damage, poor mass cell motility, greater percentages of dead/abnormal sperm and poor initial and post-freeze cell motility. The rejection rate of crossbred bulls for cryostorage of semen has been reported to be as great as 50% as a result of unacceptable semen quality. The identification of superior bulls using molecular technologies is needed which necessitates identification of the genes having a role in sperm function. The present study was, therefore, conducted to gain information on identification and expression of genes having a role in sperm motility in crossbred bulls. The gene transcripts in bulls with sperm of superior and inferior quality were profiled in Vrindavani crossbred cattle by microarray analyses and the results were verified by real time-quantitative PCR. Microarray analyses revealed 19,454 genes which were differentially expressed. At a two-fold cut off, 305 genes were differentially (P<0.01) expressed with 160 genes upregulated and 145 genes down regulated. Some of the upregulated candidate genes were further validated by RT-qPCR. These genes had a four to 16 fold upregulation in sperm with inferior motility as compared to sperm of crossbred bulls with superior motility.
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Yathish
authors.initials: Y
authors.lastname: H M
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India. Electronic address: subkum@gmail.com.
authors.forename: Subodh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Prem P
authors.initials: PP
authors.lastname: Dubey
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Rajendra P
authors.initials: RP
authors.lastname: Modi
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Rajni
authors.initials: R
authors.lastname: Chaudhary
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Siva Kumar
authors.initials: SK
authors.lastname: A
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Subrata K
authors.initials: SK
authors.lastname: Ghosh
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Mihir
authors.initials: M
authors.lastname: Sarkar
authors.affiliation: Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, 243122, India.
authors.forename: Sivamani
authors.initials: S
authors.lastname: B
mesh_terms: D000818:Animals; D001947:Breeding; D002417:Cattle / Q000235:genetics*; D003433:Crosses, Genetic; D020869:Gene Expression Profiling / Q000662:veterinary; D056726:Genetic Association Studies / Q000662:veterinary; D008297:Male; D046228:Microarray Analysis / Q000662:veterinary; D012333:RNA, Messenger / Q000032:analysis*; D012661:Semen / Q000737:chemistry* / Q000378:metabolism; D055101:Semen Analysis / Q000379:methods; D013091:Spermatogenesis / Q000235:genetics
chemical_list: D012333:RNA, Messenger
keywords: Crossbred bulls; Gene expression; Microarray; Sperm motility; Transcriptome