title: Clinicopathological features and relation between anaplastic lymphoma kinase (ALK) mutation and histological subtype of lung adenocarcinoma in Eastern European Caucasian population.
abstract: Introduction: The incidence of echinoderm microtubule-associated protein-like4-anaplastic lymphoma kinase (EML4-ALK) mutation among surgically treated patients with adenocarcinoma of the lung of the Eastern European ethnicity is underreported. The aim of this trial was the determination of EML4-ALK mutation frequency in investigated population, and the evaluation of correlations between lung adenocarcinoma subtype and clinical characteristics with mutation status.Patients and methods: This was a prospective trial which included 195 patients with adenocarcinoma of the lung who underwent surgical treatment. ALK mutation screening was performed by immunohistochemistry (IHC). IHC scores of 2+ and 3+ were regarded as positive. Confirmatory FISH was performed in all IHC positive and in 2:1 ratio in negative patients.Results: Overall ALK mutation rate established by IHC was 6.2%, while FISH confirmed rate of 5.1%. The FISH confirmed ALK positivity in 7.6% Hungarians, 5.5% Serbians, and 6.6% Slovakians. Acinar subtype of adenocarcinoma of the lung was significantly (p=0.02) related to EML4-ALK positive mutation status. Most of the patients were males (56.9%), smokers (50.8%), or former smokers (28.7%) with acinar (55.4%) or solid (35.9%) adenocarcinoma of the lung. Sensitivity and specificity of IHC were 100% and 98.9% respectively.Conclusions: ALK mutation rate in surgically treated patients with adenocarcinoma of the lung was found to be 6.2% by IHC and 5.1% by FISH. Acinar subtype of the adenocarcinoma of the lung was significantly related to ALK positive mutation.
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Bojan
authors.initials: B
authors.lastname: Zaric
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Stojsic
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Milana
authors.initials: M
authors.lastname: Panjkovic
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Dragana
authors.initials: D
authors.lastname: Tegeltija
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Vanesa
authors.initials: V
authors.lastname: Stepanov
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Tomi
authors.initials: T
authors.lastname: Kovacevic
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Sarcev
authors.affiliation: Institute of Oncology and Radiology of Serbia, Belgrade, Serbia.
authors.forename: Davorin
authors.initials: D
authors.lastname: Radosavljevic
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Aleksandar
authors.initials: A
authors.lastname: Milovancev
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Vasilis
authors.initials: V
authors.lastname: Adamidis
authors.affiliation: Pulmonary Oncology Unit, "G. Papanikolaou" General Hospital, Aristotle University of Thessaloniki, Thessaloniki, Greece.
authors.forename: Paul
authors.initials: P
authors.lastname: Zarogoulidis
authors.affiliation: Medical Clinic I, ''Fuerth'' Hospital, University of Erlangen, Fuerth, Germany.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Hohenforst-Schmidt
authors.affiliation: Division of Pulmonology, Department of Clinical Therapeutics, National and Kapodistrian University of Athens School of Medicine, Alexandra Hospital, Athens, Greece.
authors.forename: Georgia
authors.initials: G
authors.lastname: Trakada
authors.affiliation: Pulmonary Department, ''Sotiria '' Hospital of Chest Diseases, Athens, Greece.
authors.forename: Aggeliki
authors.initials: A
authors.lastname: Rapti
authors.affiliation: Institute for Pulmonary Diseases of Vojvodina, Faculty of Medicine, University of Novi Sad, Serbia.
authors.forename: Branislav
authors.initials: B
authors.lastname: Perin
keywords: EML4-ALK translocation; adenocarcinoma of the lung; anaplastic lymphoma kinase (ALK); non-small cell lung cancer