title: Novel tools for quantifying secondary growth.
abstract: Secondary growth occurs in dicotyledons and gymnosperms, and results in an increased girth of plant organs. It is driven primarily by the vascular cambium, which produces thousands of cells throughout the life of several plant species. For instance, even in the small herbaceous model plant Arabidopsis, manual quantification of this massive process is impractical. Here, we provide a comprehensive overview of current methods used to measure radial growth. We discuss the issues and problematics related to its quantification. We highlight recent advances and tools developed for automated cellular phenotyping and its future applications.
authors.affiliation: ZMBP, University of Tübingen, Auf der Morgenstelle 32, D-72076 Tübingen, Germany.
authors.forename: Anna
authors.identifier: 0000-0002-4171-2421
authors.initials: A
authors.lastname: Wunderling
authors.affiliation: ZMBP, University of Tübingen, Auf der Morgenstelle 32, D-72076 Tübingen, Germany.
authors.forename: Mehdi
authors.identifier: 0000-0002-7863-894X
authors.initials: M
authors.lastname: Ben Targem
authors.affiliation: University of Bern, Altenbergrain 21, CH-3013 Bern, Switzerland.
authors.forename: Pierre
authors.identifier: 0000-0002-9970-765X
authors.initials: P
authors.lastname: Barbier de Reuille
authors.affiliation: ZMBP, University of Tübingen, Auf der Morgenstelle 32, D-72076 Tübingen, Germany laura.ragni@zmbp.uni-tuebingen.de.
authors.forename: Laura
authors.identifier: 0000-0002-3651-8966
authors.initials: L
authors.lastname: Ragni
mesh_terms: D017360:Arabidopsis / Q000254:growth & development / Q000502:physiology; D001901:Botany / Q000379:methods; D058506:Cambium / Q000166:cytology / Q000254:growth & development / Q000502:physiology; D063245:Plant Development / Q000502:physiology
keywords: Arabidopsis; automated cellular phenotyping; machine learning; quantitative histology; secondary growth.