title: Fabrication of multiwalled carbon nanotube-polyaniline/platinum nanocomposite films toward improved performance for a cholesterol amperometric biosensor.
abstract: A simple and high sensitive cholesterol amperometric biosensor, which is based on in situ electropolymerization of multi-walled carbon nanotube-polyaniline (MWCNT-PANI) nanocomposite and electrodeposition of platinum nanoparticle (nano-Pt) films onto the glassy carbon electrode surface for cholesterol oxidase immobilization, was constructed in this study. The preparation process of the modified electrode was characterized by cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy, and chronoamperometry. Because of the synergistic electrocatalytic activity between MWCNT-PANI nanocomposites and nano-Pt, the cholesterol biosensor exhibited an excellent performance with a linear range of 2.0-510.0 µM, a detection limit of 0.8 µM (signal-to-noise ratio = 3), a high sensitivity of 109.9 µA mM-1, and a short response time within 5 Sec. Moreover, the reproducibility, stability, and selectivity of the biosensor were also investigated.
authors.affiliation: College of Chemistry and Chemical Engineering, Yibin University, Yibin, Sichuan, People's Republic of China.
authors.forename: ZeHong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: College of Chemistry and Chemical Engineering, Yibin University, Yibin, Sichuan, People's Republic of China.
authors.forename: XiaoDan
authors.initials: X
authors.lastname: Cheng
authors.affiliation: College of Chemistry and Chemical Engineering, Yibin University, Yibin, Sichuan, People's Republic of China.
authors.forename: JianHong
authors.initials: J
authors.lastname: Tan
authors.affiliation: College of Chemistry and Chemical Engineering, Yibin University, Yibin, Sichuan, People's Republic of China.
authors.forename: Xianxue
authors.initials: X
authors.lastname: Gan
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D002244:Carbon / Q000737:chemistry; D002784:Cholesterol / Q000032:analysis* / Q000097:blood / Q000737:chemistry; D004563:Electrochemistry; D004566:Electrodes; D006801:Humans; D006861:Hydrogen Peroxide / Q000032:analysis; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D053761:Nanocomposites / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D058105:Polymerization
chemical_list: D000814:Aniline Compounds; D037742:Nanotubes, Carbon; C416807:polyaniline; D010984:Platinum; D002244:Carbon; D002784:Cholesterol; D006861:Hydrogen Peroxide
keywords: biosensor; cholesterol oxidase; multiwalled carbon nanotube; platinum nanoparticles; polyaniline