title: miR-276 and miR-3016-modulated expression of acetyl-CoA carboxylase accounts for spirotetramat resistance in Aphis gossypii Glover.
abstract: Acetyl-coenzyme A carboxylase (acetyl-CoA carboxylase, ACC) catalyses the carboxylation of acetyl-CoA to produce malonyl-CoA during de novo fatty acid synthesis. A laboratory-selected spirotetramat-resistant strain (SR) of cotton aphid was used in this study. RT-qPCR results demonstrated significant increases in the levels of ACC transcript in the resistant strain compared to the susceptible strain. Depletion of overexpressed ACC transcripts by RNAi also significantly enhanced the sensitivity of the resistant aphid to spirotetramat. We hypothesized that ACC gene expression is subject to post-transcriptional regulation. To investigate the underlying mechanism, the 66 known miRNAs of Aphis gossypii were used for target prediction, eight of which were predicted to target ACC. Validation identified two miRNAs, miR-276 and miR-3016, with abundance levels that were highly inversely correlated with ACC transcript levels. This result suggests that the miRNAs miR-276 and miR-3016 may play major roles in the post-transcriptional regulation of the ACC gene. Modulation of the abundance of miR-276 and miR-3016 through addition of inhibitors/mimics of miR-276 or miR-3016 to the artificial diet significantly altered both ACC transcript levels and the tolerance of A. gossypii to spirotetramat, thus confirming the roles of these two miRNAs in the regulation of spirotetramat resistance.
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Wei
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zheng
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Tianfei
authors.initials: T
authors.lastname: Peng
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Yiou
authors.initials: Y
authors.lastname: Pan
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Xi
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, PR China.
authors.forename: Xuewei
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Juhong
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Entomology, China Agricultural University, Beijing 100193, PR China. Electronic address: gaoxiwu@263.net.cn.
authors.forename: Xiwu
authors.initials: X
authors.lastname: Gao
authors.affiliation: College of Plant Science, Jilin University, Changchun 130062, PR China. Electronic address: shangqingli@163.com.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Shang
keywords: Acetyl-CoA carboxylase; Aphis gossypii; MicroRNA; Spirotetramat