title: Novel electrospun nanofibers of modified gelatin-tyrosine in cartilage tissue engineering.
abstract: In natural cartilage tissues, chondrocytes are linked to extracellular matrix (ECM) through cell-surface binding proteins. Surface modification of gelatin can provide a new generation of biopolymers and fibrous scaffolds with chemical, mechanical, and biological properties. In this study tyrosine protein and 1,2,3-triazole ring were utilized to functionalize gelatin without Cu catalyst. Their molecular structure was characterized by Fourier transform infrared spectroscopy (FTIR) and nuclear magnetic resonance spectroscopy (1HNMR). Chemical cross-linkers such as glutaraldehyde (GA) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-hydroxysulfosuccinimide (NHS) were used to electrospin the modified gelatin. The modification of gelatin and cross-linking effects were confirmed by scanning electron microscopy (SEM), contact angle measurement, and mechanical tests. MTT assay using chondrocyte cells showed cell viability of electrospun modified gelatin scaffolds. In vitro cell culture studies showed that electrospun engineered protein scaffolds would support the attachment and growth of cells. The results also showed that cross-linked nanofibers with EDC/NHS could be considered excellent matrices in cell adhesion and proliferation before electrospinning process and their potential substrate in tissue engineering applications, especially in the field of cartilage engineering.
authors.affiliation: Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan 81744176, Iran.
authors.forename: Maria
authors.initials: M
authors.lastname: Agheb
authors.affiliation: Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Dinari
authors.affiliation: Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan 81744176, Iran. Electronic address: m_rafienia@med.mui.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rafienia
authors.affiliation: Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan 81744176, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Salehi
mesh_terms: D000818:Animals; D002356:Cartilage; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D005780:Gelatin / Q000737:chemistry / Q000494:pharmacology; D057139:Nanofibers / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering; D014443:Tyrosine / Q000737:chemistry / Q000494:pharmacology
chemical_list: D014443:Tyrosine; D005780:Gelatin
keywords: Cartilage tissue engineering; Gelatin; Nanofibers; Triazole; Tyrosine