title: The MYB107 Transcription Factor Positively Regulates Suberin Biosynthesis.
abstract: Suberin, a lipophilic polymer deposited in the outer integument of the Arabidopsis (Arabidopsis thaliana) seed coat, represents an essential sealing component controlling water and solute movement and protecting seed from pathogenic infection. Although many genes responsible for suberin synthesis are identified, the regulatory components controlling its biosynthesis have not been definitively determined. Here, we show that the Arabidopsis MYB107 transcription factor acts as a positive regulator controlling suberin biosynthetic gene expression in the seed coat. MYB107 coexpresses with suberin biosynthetic genes in a temporal manner during seed development. Disrupting MYB107 particularly suppresses the expression of genes involved in suberin but not cutin biosynthesis, lowers seed coat suberin accumulation, alters suberin lamellar structure, and consequently renders higher seed coat permeability and susceptibility to abiotic stresses. Furthermore, MYB107 directly binds to the promoters of suberin biosynthetic genes, verifying its primary role in regulating their expression. Identifying MYB107 as a positive regulator for seed coat suberin synthesis offers a basis for discovering the potential transcriptional network behind one of the most abundant lipid-based polymers in nature.
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973 (M.G., H.Y., X.Z., Y.C., G.K., C.-J.L.); and.|Appalachian State University, Boone, North Carolina 28608-2027 (G.H.).
authors.forename: Mingyue
authors.identifier: 0000-0001-8855-6617
authors.initials: M
authors.lastname: Gou
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973 (M.G., H.Y., X.Z., Y.C., G.K., C.-J.L.); and.|Appalachian State University, Boone, North Carolina 28608-2027 (G.H.).
authors.forename: Guichuan
authors.identifier: 0000-0002-3856-056X
authors.initials: G
authors.lastname: Hou
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973 (M.G., H.Y., X.Z., Y.C., G.K., C.-J.L.); and.|Appalachian State University, Boone, North Carolina 28608-2027 (G.H.).
authors.forename: Huijun
authors.initials: H
authors.lastname: Yang
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973 (M.G., H.Y., X.Z., Y.C., G.K., C.-J.L.); and.|Appalachian State University, Boone, North Carolina 28608-2027 (G.H.).
authors.forename: Xuebin
authors.identifier: 0000-0002-9012-8491
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973 (M.G., H.Y., X.Z., Y.C., G.K., C.-J.L.); and.|Appalachian State University, Boone, North Carolina 28608-2027 (G.H.).
authors.forename: Yuanheng
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973 (M.G., H.Y., X.Z., Y.C., G.K., C.-J.L.); and.|Appalachian State University, Boone, North Carolina 28608-2027 (G.H.).
authors.forename: Guoyin
authors.initials: G
authors.lastname: Kai
authors.affiliation: Biology Department, Brookhaven National Laboratory, Upton, New York 11973 (M.G., H.Y., X.Z., Y.C., G.K., C.-J.L.); and cliu@bnl.gov.|Appalachian State University, Boone, North Carolina 28608-2027 (G.H.) cliu@bnl.gov.
authors.forename: Chang-Jun
authors.identifier: 0000-0001-6189-8756
authors.initials: CJ
authors.lastname: Liu
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D053898:Biosynthetic Pathways / Q000235:genetics; D002467:Cell Nucleus / Q000378:metabolism; D047369:Chromatin Immunoprecipitation; D002851:Chromatography, High Pressure Liquid; D018744:DNA, Plant / Q000378:metabolism; D008401:Gas Chromatography-Mass Spectrometry; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D008055:Lipids / Q000096:biosynthesis*; D008563:Membrane Lipids / Q000378:metabolism; D009154:Mutation / Q000235:genetics; D010539:Permeability; D019441:Plant Epidermis / Q000378:metabolism / Q000648:ultrastructure; D011401:Promoter Regions, Genetic; D060888:Real-Time Polymerase Chain Reaction; D012639:Seeds / Q000378:metabolism / Q000648:ultrastructure; D017423:Sequence Analysis, RNA; D013312:Stress, Physiological; D013347:Subcellular Fractions / Q000378:metabolism; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D018744:DNA, Plant; D008055:Lipids; C000622994:MYB107 protein, Arabidopsis; D008563:Membrane Lipids; D014157:Transcription Factors; C000521:cutin; C065875:suberin