title: Development and validation of a highly sensitive quantitative/confirmatory method for the determination of ivermectin residues in bovine tissues by UHPLC-MS/MS.
abstract: In this work, a highly sensitive quantitative/confirmatory method was developed for the selective determination of marker residue 22,23-dihydroavermectin B1ain fat, kidney, liver and muscle bovine tissues using UHPLC-MS/MS. A QuEChERS method and a subsequent d-SPE cleaning with MgSO4and C18 were used for samples preparation. The method was validated at three levels in each tissue (8.0, 20.0 and 80.0μg/kg for fat, kidney and muscle, 8.0, 16.0 and 40.0μg/kg for liver), taking into account international guidelines. Linearity and matrix effect, accuracy, within-laboratory repeatability, specificity, LOQ, LOD, Q/q relative ion intensities and uncertainty were reported. Recoveries were obtained in the range of 70-120% with within-laboratory repeatability less than 20% for the overall working range. LODs varied from 0.05 to 0.12μg/kg. Compound identification was performed using the relative intensities of monitored transitions. The method could be applied to monitoring withdrawal period specified for ivermectin in cattle.
authors.affiliation: GDCON Research Group, Faculty of Engineering, University Research Headquarters (SIU), University of Antioquia, Street 70 # 52-21, Medellin, Colombia. Electronic address: duvan.hoyos@udea.edu.co.
authors.forename: Duvan E
authors.initials: DE
authors.lastname: Hoyos O
authors.affiliation: GDCON Research Group, Faculty of Engineering, University Research Headquarters (SIU), University of Antioquia, Street 70 # 52-21, Medellin, Colombia.
authors.forename: Yudy A
authors.initials: YA
authors.lastname: Cuartas O
authors.affiliation: GDCON Research Group, Faculty of Engineering, University Research Headquarters (SIU), University of Antioquia, Street 70 # 52-21, Medellin, Colombia.
authors.forename: Gustavo A
authors.initials: GA
authors.lastname: Peñuela M
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry; D000818:Animals; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D007559:Ivermectin / Q000031:analogs & derivatives* / Q000032:analysis; D007668:Kidney / Q000737:chemistry*; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D007559:Ivermectin; C052102:22,23-dihydroavermectin B(1)a
keywords: 22,23-Dihydroavermectin B(1a) (PubChem CID: 6427057); 22,23-Dihydroavermectin B(1b) (PubChem CID: 6321425); Bovine tissues; Ivermectin; Marker residue H(2)B(1a); QuEChERS; UHPLC–MS/MS; Withdrawal period