title: Mycobacterium tuberculosis PE_PGRS18 enhances the intracellular survival of M. smegmatis via altering host macrophage cytokine profiling and attenuating the cell apoptosis.
abstract: Mycobacterium tuberculosis PE/PPE family proteins, named after the presence of conserved PE (Pro-Glu) and PPE (Pro-Pro-Glu) domains at N-terminal, are prevalent in M. tuberculosis genome. The function of most PE/PPE family proteins remains elusive. To characterize the function of PE_PGRS18, the encoding gene was heterologously expressed in M. smegmatis, a nonpathogenic mycobacterium. The recombinant PE_PGRS18 is cell wall associated. M. smegmatis PE_PGRS18 recombinant showed differential response to stresses and altered the production of host cytokines IL-6, IL-1β, IL-12p40 and IL-10, as well as enhanced survival within macrophages largely via attenuating the apoptosis of macrophages. In summary, the study firstly unveiled the role of PE_PGRS18 in physiology and pathogenesis of mycobacterium.
authors.affiliation: State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University, Chongqing, China.
authors.forename: Wenmin
authors.initials: W
authors.lastname: Yang
authors.affiliation: Key Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, the Second Affiliated Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Wanyan
authors.initials: W
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics, Schoolof Public Health, Xiamen University, Xiang-An, Xiamen, Fujian, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zeng
authors.affiliation: State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University, Chongqing, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Ren
authors.affiliation: State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University, Chongqing, China.
authors.forename: Md Kaisar
authors.initials: MK
authors.lastname: Ali
authors.affiliation: State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University, Chongqing, China.
authors.forename: Yinzhong
authors.initials: Y
authors.lastname: Gu
authors.affiliation: State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University, Chongqing, China.
authors.forename: Yangyuling
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, School of Life Sciences, Institute of Modern Biopharmaceuticals, Southwest University, Chongqing, China. georgex@swu.edu.cn.
authors.forename: Jianping
authors.initials: J
authors.lastname: Xie
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D001426:Bacterial Proteins / Q000235:genetics / Q000502:physiology*; D016207:Cytokines / Q000096:biosynthesis*; D005798:Genes, Bacterial; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000382:microbiology*; D008565:Membrane Proteins / Q000235:genetics / Q000502:physiology*; D051379:Mice; D020102:Mycobacterium smegmatis / Q000166:cytology / Q000502:physiology*; D009169:Mycobacterium tuberculosis / Q000166:cytology / Q000235:genetics / Q000502:physiology*; D009569:Nitric Oxide / Q000378:metabolism; D000072417:Protein Domains; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D000074084:THP-1 Cells; D015854:Up-Regulation
chemical_list: D000942:Antigens, Bacterial; D001426:Bacterial Proteins; D016207:Cytokines; D008565:Membrane Proteins; C440642:PE-PGRS protein, Mycobacterium; C000626413:PE_PGRS18 protein, Mycobacterium tuberculosis; D011993:Recombinant Fusion Proteins; D009569:Nitric Oxide
keywords: Apoptosis; Cytokine; Mycobacterium tuberculosis; PE-PGRS family; PE_PGRS 18