title: Functional characterization and efficient detection of Nucleophosmin/NPM1 oligomers.
abstract: NPM1/nucleophosmin is a multifunctional and oligomeric phosphoprotein. A number of observations have suggested that changes in the oligomer formation of NPM1 could influence its biological functions, especially its oncogenic functions. To understand the functional meaning of oligomerization of NPM1/nucleophosmin, we have established a novel method to monitor protein oligomerization in cells. We utilized the split synthetic Renilla luciferase protein fragment-assisted complementation (SRL-PFAC) bioluminescence activity and observed the change of NPM1 oligomer levels under various cell culture conditions. Our study provides a method for systematic characterization of NPM1 oligomer formation changes and for screening inhibitors of NPM1 oligomerization.
authors.affiliation: PhD Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, 305-8575, Japan; Department of Infection Biology, Faculty of Medicine, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, 305-8575, Japan.
authors.forename: Jianhuang
authors.initials: J
authors.lastname: Lin
authors.affiliation: Department of Infection Biology, Faculty of Medicine, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, 305-8575, Japan.
authors.forename: Miharu
authors.initials: M
authors.lastname: Hisaoka
authors.affiliation: Department of Infection Biology, Faculty of Medicine, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, 305-8575, Japan.
authors.forename: Kyosuke
authors.initials: K
authors.lastname: Nagata
authors.affiliation: PhD Program in Human Biology, School of Integrative and Global Majors, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, 305-8575, Japan; Department of Infection Biology, Faculty of Medicine, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, 305-8575, Japan. Electronic address: mokuwaki@md.tsukuba.ac.jp.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Okuwaki
mesh_terms: D001665:Binding Sites; D019281:Dimerization; D057809:HEK293 Cells; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D009687:Nuclear Proteins / Q000378:metabolism*; D000090243:Nucleophosmin; D056489:Nucleoplasmins / Q000378:metabolism*; D011485:Protein Binding; D025941:Protein Interaction Mapping
chemical_list: C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D056489:Nucleoplasmins; D000090243:Nucleophosmin
keywords: Histone chaperone; Nucleolus; Nucleosome assembly; Ribosome biogenesis