title: Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing.
abstract: Bermuda grass samples were examined by transmission electron microscopy and 28-30 nm spherical virus particles were observed. Total RNA from these plants was subjected to high-throughput sequencing (HTS). The nearly full genome sequence of a panicovirus was identified from one HTS scaffold. Sanger sequencing was used to confirm the HTS results and complete the genome sequence of 4404 nt. This virus was provisionally named Bermuda grass latent virus (BGLV). Its predicted open reading frames follow the typical arrangement of the genus Panicovirus. Based on sequence comparisons and phylogenetic analyses BGLV differs from other viruses and therefore taxonomically it is a new member of the genus Panicovirus, family Tombusviridae.
authors.affiliation: USDA, ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.
authors.forename: Muhammad N
authors.initials: MN
authors.lastname: Tahir
authors.affiliation: Department of Plant Pathology, University of Minnesota, St. Paul, MN, 55108, USA.
authors.forename: Ben
authors.initials: B
authors.lastname: Lockhart
authors.affiliation: USDA, ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA.
authors.forename: Samuel
authors.initials: S
authors.lastname: Grinstead
authors.affiliation: USDA, ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA. dimitre.mollov@ars.usda.gov.
authors.forename: Dimitre
authors.identifier: http://orcid.org/0000-0002-4750-6194
authors.initials: D
authors.lastname: Mollov
mesh_terms: D001483:Base Sequence; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology; D006109:Poaceae / Q000821:virology*; D012367:RNA, Viral; D019183:Tombusviridae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D014764:Viral Proteins / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins