title: A Collaborative Study: Determination of Mycotoxins in Corn, Peanut Butter, and Wheat Flour Using Stable Isotope Dilution Assay (SIDA) and Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).
abstract: A collaborative study was conducted to evaluate stable isotope dilution assay (SIDA) and LC-MS/MS for the simultaneous determination of aflatoxins B1, B2, G1, and G2; deoxynivalenol; fumonisins B1, B2, and B3; ochratoxin A; HT-2 toxin; T-2 toxin; and zearalenone in foods. Samples were fortified with 1213C uniformly labeled mycotoxins (13C-IS) corresponding to the native mycotoxins and extracted with acetonitrile/water (50:50 v/v), followed by centrifugation, filtration, and LC-MS/MS analysis. In addition to certified reference materials, the six participating laboratories analyzed corn, peanut butter, and wheat flour fortified with the 12 mycotoxins at concentrations ranging from 1.0 to 1000 ng/g. Using their available LC-MS/MS platform, each laboratory developed in-house instrumental conditions for analysis. The majority of recoveries ranged from 80 to 120% with relative standard derivations (RSDs) <20%. Greater than 90% of the average recoveries of the participating laboratories were in the range of 90-110%, with repeatability RSDr(within laboratory) < 10% and reproducibility RSDR(among laboratory) < 15%. All Z scores of the results of certified reference materials were between -2 and 2. Using13C-IS eliminated the need for matrix-matched calibration standards for quantitation, simplified sample preparation, and achieved simultaneous identification and quantitation of multiple mycotoxins in a simple LC-MS/MS procedure.
authors.affiliation: Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States.
authors.forename: Kai
authors.identifier: http://orcid.org/0000-0001-5668-9458
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Arizona Department of Health Services , 250 North 17th Avenue, Phoenix, Arizona 85007, United States.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Schaab
authors.affiliation: Arizona Department of Health Services , 250 North 17th Avenue, Phoenix, Arizona 85007, United States.
authors.forename: Gavin
authors.initials: G
authors.lastname: Southwood
authors.affiliation: California Animal Health and Food Safety Laboratory System, Toxicology Laboratory, Univerisity of California-Davis , West Health Sciences Drive, Davis, California 95616, United States.
authors.forename: Elizabeth R
authors.initials: ER
authors.lastname: Tor
authors.affiliation: California Animal Health and Food Safety Laboratory System, Toxicology Laboratory, Univerisity of California-Davis , West Health Sciences Drive, Davis, California 95616, United States.
authors.forename: Linda S
authors.initials: LS
authors.lastname: Aston
authors.affiliation: Food & Drug Laboratory Branch, California Department of Public Health , 850 Marina Bay Parkway, G365, Richmond, California 94804, United States.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Analytical Chemistry, Connecticut Agricultural Experiment Station , 123 Huntington Street, New Haven, Connecticut 06511, United States.
authors.forename: Brian
authors.initials: B
authors.lastname: Eitzer
authors.affiliation: Department of Analytical Chemistry, Connecticut Agricultural Experiment Station , 123 Huntington Street, New Haven, Connecticut 06511, United States.
authors.forename: Sanghamitra
authors.initials: S
authors.lastname: Majumdar
authors.affiliation: Pacific Regional Laboratory Northwest, U.S. Food and Drug Administration 22201 23rd Drive S.E., Bothell, Washington 98021, United States.
authors.forename: Theodore
authors.initials: T
authors.lastname: Lapainis
authors.affiliation: Kansas City Laboratory, U.S. Food and Drug Administration , 11510 West 80th Street, Lenexa, Kansas 66214, United States.
authors.forename: Huy
authors.initials: H
authors.lastname: Mai
authors.affiliation: Kansas City Laboratory, U.S. Food and Drug Administration , 11510 West 80th Street, Lenexa, Kansas 66214, United States.
authors.forename: Kevin
authors.initials: K
authors.lastname: Tran
authors.affiliation: Kansas City Laboratory, U.S. Food and Drug Administration , 11510 West 80th Street, Lenexa, Kansas 66214, United States.
authors.forename: Aref
authors.initials: A
authors.lastname: El-Demerdash
authors.affiliation: Southeast Regional Laboratory, U.S. Food and Drug Administration , 60 8th Street, Atlanta, Georgia 30309, United States.
authors.forename: Victor
authors.initials: V
authors.lastname: Vega
authors.affiliation: Office of Regulatory Affairs, U.S. Food and Drug Administration , 12420 Parklawn Drive Element Building, Rockville, Maryland 20857, United States.
authors.forename: Yanxuan
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States.
authors.forename: Jon W
authors.initials: JW
authors.lastname: Wong
authors.affiliation: Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States.
authors.forename: Alexandra J
authors.initials: AJ
authors.lastname: Krynitsky
authors.affiliation: Center for Food Safety and Applied Nutrition, Office of Regulatory Science, U.S. Food and Drug Administration , 5001 Campus Drive, College Park, Maryland 20740, United States.
authors.forename: Timothy H
authors.initials: TH
authors.lastname: Begley
mesh_terms: D010367:Arachis / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005433:Flour / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis*; D007201:Indicator Dilution Techniques; D009183:Mycotoxins / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D009183:Mycotoxins
keywords: LC-MS/MS; mycotoxin; stable isotope dilution assay (SIDA)