title: Simple and Cost-Effective Glucose Detection Based on Carbon Nanodots Supported on Silver Nanoparticles.
abstract: We present a new glucose oxidase (GOx)-mediated strategy for detecting glucose based on carbon nanodots supported on silver nanoparticles (C-dots/AgNPs) as nanocomplexes. The strategy involves three processes: quenching of C-dots' fluorescence by AgNPs, production of H2O2from GOx-catalyzed oxidation of glucose, and H2O2-induced etching of AgNPs. In the C-dots/AgNPs complex, AgNPs act as a "nanoquencher" to decrease C-dots fluorescence by surface plasmon-enhanced energy transfer (SPEET) from C-dots (donor) to AgNPs (acceptor). The H2O2formed by GOx-catalyzed oxidation of glucose etches the AgNPs to silver ions, thus freeing the C-dots from the AgNPs surfaces and restoring the C-dots' fluorescence. Therefore, the increase in fluorescence depends directly on the concentration of H2O2, which, in turn, depends on the concentration of glucose. The strategy allows the quantitative analysis of glucose with a detection limit of 1.39 μM. The method based on C-dots/AgNPs offers the following advantages: simplicity of design and facile preparation of nanomaterials, as well as low experimental cost, because chemical modification and separation procedures are not needed.
authors.affiliation: Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology , Shanghai 200237, China.
authors.forename: Jin-Liang
authors.initials: JL
authors.lastname: Ma
authors.affiliation: Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology , Shanghai 200237, China.
authors.forename: Bin-Cheng
authors.identifier: 0000-0002-4011-4307
authors.initials: BC
authors.lastname: Yin
authors.affiliation: Department of Rheumatology and Immunology, Shanghai Changzheng Hospital, The Second Military Medical University , Shanghai 200433, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wu
authors.affiliation: Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science & Technology , Shanghai 200237, China.|School of Chemistry and Chemical Engineering, Shihezi University , Xinjiang 832000, China.
authors.forename: Bang-Ce
authors.initials: BC
authors.lastname: Ye