title: Fibroblast growth factor 18 promotes proliferation and migration of H460 cells via the ERK and p38 signaling pathways.
abstract: Recently, fibroblast growth factor 18 (FGF18) expression was reported to be upregulated in colon cancer and ovarian cancer, and increased expression of FGF18 mRNA and protein is associated with tumor progression and poor overall survival in patients; however, its role in lung cancer remains to be explored. In the present study, the effect and underlying molecular mechanisms of FGF18 on H460 cells were investigated. Cell proliferation and cell cycle alterations were detected using a 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay and flow cytometry. A wound healing assay was conducted to detect cell migration. Reverse transcription-quantitative polymerase chain reaction and western blotting were performed to measure extracellular signal-regulated kinase (ERK), p38 and matrix metalloproteinase 26 (MMP26) expression. Knockdown of FGF18 using short interfering RNA (siRNA-FGF18) suppressed H460 cell proliferation, inhibited cell migration via the downregulation of MMP26 levels, with siRNA-FGF18 additionally inhibiting the ERK and p38 signaling pathway. The present study indicates that FGF18 serves an essential role in the growth and migration of non-small cell lung cancer (NSCLC) cells by regulating the ERK, p38 signaling pathways and MMP26 protein levels, suggesting that FGF18 may be a potential molecular drug target for the treatment NSCLC.
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Taotao
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Weiyue
authors.initials: W
authors.lastname: Gong
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Haishan
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Pathology, Xinxiang Medical University, Xinxiang, Henan 453000, P.R. China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Shenghui
authors.initials: S
authors.lastname: Chu
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Jisheng
authors.initials: J
authors.lastname: Ma
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Huanhuan
authors.initials: H
authors.lastname: Yang
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Jiliang
authors.initials: J
authors.lastname: Cheng
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Jiang
mesh_terms: D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002453:Cell Cycle; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005346:Fibroblast Growth Factors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D020935:MAP Kinase Signaling System; D053505:Matrix Metalloproteinases, Secreted / Q000378:metabolism; D034741:RNA, Small Interfering; D015398:Signal Transduction; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D034741:RNA, Small Interfering; C113569:fibroblast growth factor 18; D005346:Fibroblast Growth Factors; D048051:p38 Mitogen-Activated Protein Kinases; C410599:MMP26 protein, human; D053505:Matrix Metalloproteinases, Secreted