title: Kinetic stability and cellular uptake of lutein in WPI-stabilised nanoemulsions and emulsions prepared by emulsification and solvent evaporation method.
abstract: The particle size and lutein encapsulation efficiency of nanoemulsions prepared by emulsification and solvent evaporation method were 68.8±0.3nm and 80.7±0.8%, respectively, whereas they were 147.3±0.6nm and 86.3±0.3% for conventional emulsions. All the emulsions had no change in their particle size during storage (28days at 5, 20 and 40°C) but their lutein content and emulsion colour decreased, especially at 40°C. The lutein emulsions were analysed using MTT assay on the gut enterocyte cell line Caco-2 and they showed no toxicity as the cell viability was more than 80% at 10times or higher dilution after 24h of incubation. However, there was a higher cellular uptake of lutein by Caco-2 cells in nanoemulsions (872.9±88.3pmol/mgprotein) than conventional emulsions (329.5±214.6pmol/mgprotein). The results of this study indicated that nanoemulsions can be used as a delivery system to improve the cellular uptake of lutein.
authors.affiliation: School of Food and Nutrition, Massey University, Auckland, New Zealand; School of Food and Nutrition, Massey University, Palmerston North, New Zealand.
authors.forename: Anges
authors.initials: A
authors.lastname: Teo
authors.affiliation: School of Food and Nutrition, Massey University, Auckland, New Zealand. Electronic address: s.j.lee@massey.ac.nz.
authors.forename: Sung Je
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: School of Food and Nutrition, Massey University, Palmerston North, New Zealand.
authors.forename: Kelvin K T
authors.initials: KKT
authors.lastname: Goh
authors.affiliation: School of Food and Nutrition, Massey University, Palmerston North, New Zealand.
authors.forename: Frances M
authors.initials: FM
authors.lastname: Wolber
mesh_terms: D018938:Caco-2 Cells; D004339:Drug Compounding / Q000379:methods*; D004355:Drug Stability; D004655:Emulsions / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D007700:Kinetics; D014975:Lutein / Q000737:chemistry* / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D010316:Particle Size; D012997:Solvents; D000067816:Whey Proteins / Q000737:chemistry*
chemical_list: D004655:Emulsions; D012997:Solvents; D000067816:Whey Proteins; D014975:Lutein
keywords: Cellular uptake; Cytotoxicity; Emulsions; Encapsulation; Kinetic stability; Lutein; Nanoemulsions