title: Identification ofITGA2BandITGB3Single-Nucleotide Polymorphisms and Their Influences on the Platelet Function.
abstract: The aim of the study was to investigateITGA2BandITGB3genetic polymorphisms and to evaluate the variability in the platelet function in healthy Chinese subjects. The genetic sequence of the entire coding region of theITGA2BandITGB3genes was investigated. Adenosine diphosphate-induced platelet aggregation, glycoprotein IIb/IIIa content, bleeding time, and coagulation indexes were detected. Thirteen variants in theITGA2Blocus and 29 variants in theITGB3locus were identified in the Chinese population. The rs1009312 and rs2015049 were associated with the mean platelet volume. The rs70940817 was significantly correlated with the prothrombin time. The rs70940817 and rs112188890 were related with the activated partial thromboplastin time, andITGB3rs4642 was correlated with the thrombin time and fibrinogen. The minor alleles of rs56197296 and rs5919 were associated with decreased ADP-induced platelet aggregation, and rs55827077 was related with decreased GPIIb/IIIa per platelet. The rs1009312, rs2015049, rs3760364, rs567581451, rs7208170, and rs117052258 were related with bleeding time. Further studies are needed to explore the clinical importance ofITGA2BandITGB3SNPs in the platelet function.
authors.affiliation: Department of Pharmacy, Base for Clinical Trial, Peking University First Hospital, Beijing 100034, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Xiang
authors.affiliation: Jiangsu Institute of Hematology, Key Laboratory of Thrombosis and Hemostasis of Ministry of Health, The First Affiliated Hospital of Soochow University, Suzhou 215006, China; Collaborative Innovation Center of Hematology, Soochow University, Suzhou 215006, China.
authors.forename: Shun-Dong
authors.initials: SD
authors.lastname: Ji
authors.affiliation: Department of Pharmacy, Base for Clinical Trial, Peking University First Hospital, Beijing 100034, China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Pharmacy, Base for Clinical Trial, Peking University First Hospital, Beijing 100034, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Pharmacy, Base for Clinical Trial, Peking University First Hospital, Beijing 100034, China.
authors.forename: Yi-Min
authors.identifier: 0000-0002-4186-1005
authors.initials: YM
authors.lastname: Cui
mesh_terms: D000293:Adolescent; D000328:Adult; D000483:Alleles; D044466:Asian People / Q000235:genetics; D001760:Bleeding Time / Q000379:methods; D001777:Blood Coagulation / Q000235:genetics; D001792:Blood Platelets / Q000502:physiology*; D005340:Fibrinogen / Q000235:genetics; D005838:Genotype; D006801:Humans; D039421:Integrin alpha2 / Q000235:genetics*; D039661:Integrin beta3 / Q000235:genetics*; D063847:Mean Platelet Volume / Q000379:methods; D008875:Middle Aged; D016366:Open Reading Frames / Q000235:genetics; D010974:Platelet Aggregation / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011517:Prothrombin Time / Q000379:methods; D055815:Young Adult
chemical_list: C056564:ITGA2B protein, human; C061248:ITGB3 protein, human; D039421:Integrin alpha2; D039661:Integrin beta3; D005340:Fibrinogen