title: A novel pretreatment method combining sealing technique with direct injection technique applied for improving biosafety.
abstract: OBJECTIVE
  People today have a stronger interest in the risk of biosafety in clinical bioanalysis. A safe, simple, effective method of preparation is needed urgently.
  
  
  RESULTS
  To improve biosafety of clinical analysis, we used antiviral drugs of adefovir and tenofovir as model drugs and developed a safe pretreatment method combining sealing technique with direct injection technique. The inter- and intraday precision (RSD %) of the method were <4%, and the extraction recoveries ranged from 99.4 to 100.7%. Meanwhile, the results showed that standard solution could be used to prepare calibration curve instead of spiking plasma, acquiring more accuracy result.
  
  
  CONCLUSIONS
  Compared with traditional methods, the novel method not only improved biosecurity of the pretreatment method significantly, but also achieved several advantages including higher precision, favorable sensitivity and satisfactory recovery. With these highly practical and desirable characteristics, the novel method may become a feasible platform in bioanalysis.
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Jing-Lin
authors.initials: JL
authors.lastname: Gao
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Chaohui
authors.initials: C
authors.lastname: Du
authors.affiliation: Department of Pharmacy, Hebei General Hospital, Shijiazhuang, Hebei Province 050051, China.
authors.forename: Jing
authors.initials: J
authors.lastname: An
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: MengJiao
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Ma
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang, Hebei Province 050017, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D000225:Adenine / Q000031:analogs & derivatives* / Q000097:blood / Q000302:isolation & purification; D000998:Antiviral Agents / Q000097:blood* / Q000302:isolation & purification; D002851:Chromatography, High Pressure Liquid; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000379:methods* / Q000592:standards; D006801:Humans; D057230:Limit of Detection; D063065:Organophosphonates / Q000097:blood* / Q000302:isolation & purification; D000068698:Tenofovir / Q000097:blood* / Q000302:isolation & purification; D014462:Ultrafiltration / Q000295:instrumentation; D014777:Virus Diseases / Q000517:prevention & control
chemical_list: D000998:Antiviral Agents; D063065:Organophosphonates; C053001:adefovir; D000068698:Tenofovir; D000225:Adenine
keywords: antiviral drug; biosafety; clinical bioanalysis; high infectious pathogenic microorganisms; hollow fiber centrifugal ultrafiltration; micro-enclosed system