title: A novel LC-MS/MS method for mepivacaine determination and pharmacokinetic study in a single-dose two-period crossover in healthy subjects.
abstract: The objective of this work was to develop a simple, selective, and sensitive LC-MS/MS method for the quantitation of the mepivacaine in Chinese biological matrix. The calibration curve of mepivacaine ranged from 0.5 to 2000 ng/mL with the lower limit of quantitation being 0.5 ng/mL. This sensitivity was high enough to describe the profile of blood mepivacaine level versus time. Thereby it was very desirable for the pharmacokinetic study because of its high sensitivity and accuracy. The study used a single-dose two-period crossover design principle. For the pharmacokinetic analysis of plasma, the mean (SD) values obtained were as follows: t1/2, 1.63 (0.43) h; Cmax, 435.3 (67.4) ng/ml; AUC0-t, 1546.9 (339.7) ng/ml·h; AUC0-∞, 1982.3 (421.4) ng/ml·h; Tmax, 0.62 (0.31) h. The validated method has been successfully applied to assess the pharmacokinetic study of mepivacaine after a single administration to Chinese volunteers.
authors.affiliation: a Department of General Surgery , The Second Affiliated Hospital of Soochow University , Suzhou , China.|b Department of Anesthesiology, Shanghai Pulmonary Hospital , Tongji University School of Medicine , Shanghai , China.
authors.forename: Ruo-Wang
authors.initials: RW
authors.lastname: Duan
authors.affiliation: b Department of Anesthesiology, Shanghai Pulmonary Hospital , Tongji University School of Medicine , Shanghai , China.
authors.forename: Jiong
authors.initials: J
authors.lastname: Song
authors.affiliation: b Department of Anesthesiology, Shanghai Pulmonary Hospital , Tongji University School of Medicine , Shanghai , China.
authors.forename: Yu-Ping
authors.initials: YP
authors.lastname: Li
authors.affiliation: a Department of General Surgery , The Second Affiliated Hospital of Soochow University , Suzhou , China.
authors.forename: Chun-Gen
authors.initials: CG
authors.lastname: Xing
mesh_terms: D001774:Blood Chemical Analysis / Q000379:methods*; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D064368:Healthy Volunteers; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D008297:Male; D008619:Mepivacaine / Q000097:blood* / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014018:Tissue Distribution
chemical_list: D008619:Mepivacaine
keywords: Chinese; LC–MS/MS; mepivacaine; pharmacokinetics