title: Hydroxycamptothecin Nanorods Prepared by Fluorescently Labeled Oligoethylene Glycols (OEG) Codendrimer: Antitumor Efficacy in Vitro and in Vivo.
abstract: Nanorods based on dendrimers were explored as excellent candidates for nanoscale drug delivery system. In this study, fluorescently labeled PAMAM-b-oligoethylene glycols codendrimer (POC) was utilized as carrier to prepare 10-hydroxycamptothecin (HCPT) loaded nanorods (HCPT NRs) via antisolvent precipitation method augmented by ultrasonication with the optimized drug-loading content (∼90.6%) and positive charged surface. The nanorods presented high stability, and the release of HCPT nanorods showed a sustained release manner and was completed within 48 h. The nanorods presented higher cytotoxicity against HepG2 and 4T1 cells than HCPT injections, and the cellular uptake mechanism was proved to involve clathrin-mediated endocytosis and macropincytosis-dependent endobytosis. Importantly, the HCPT nanorods resulted in strong antitumor efficacy on the H22 liver tumor model, and no significant adverse effects was observed. Besides, in vivo studies also showed that HCPT NRs possessed better tumor accumulation over HCPT injection at the equivalent concentration. According to the high drug-loading content, enhanced antitumor efficacy, and appropriate particle size, HCPT NRs as the safe and efficient drug delivery systems could have potential application for cancer chemotherapy in clinic.
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Yifei
authors.identifier: 0000-0003-4548-5040
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Li
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Meihua
authors.initials: M
authors.lastname: Han
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Zhengqi
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College , No. 151, Malianwa North Road, Haidian District, Beijing 100193, China.
authors.forename: Xiangtao
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology; D001692:Biological Transport; D002166:Camptothecin / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology; D050091:Dendrimers / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism / Q000493:pharmacokinetics; D065546:Drug Liberation; D004355:Drug Stability; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008297:Male; D051379:Mice; D043942:Nanotubes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D014018:Tissue Distribution
chemical_list: D000970:Antineoplastic Agents; D050091:Dendrimers; D004337:Drug Carriers; D005456:Fluorescent Dyes; C104700:PAMAM Starburst; D012997:Solvents; D011092:Polyethylene Glycols; C028098:10-hydroxycamptothecin; D002166:Camptothecin