title: MiR-141-3p promotes prostate cancer cell proliferation through inhibiting kruppel-like factor-9 expression.
abstract: Evidence has revealed that some microRNAs play a critical role in tumor proliferation. We demonstrated that miR-141-3p appears to be a novel oncogene miRNA, which promotes prostate tumorigenesis and facilitates the stemness of prostate cancer cells via suppressing a key transcription factor kruppel-like factor-9 (KLF9). KLF9 is the core effector protein that might suppress tumor growth. MiR-141-3p is upregulated in prostate cancer cells and tissues compared to non-tumorigenic prostate epithelial cells and prostate tissues. MiR-141-3p positively regulated proliferation, spheroid formation, and expression of the stemness factors OCT-4, Nanog, SOX-9, Bmil, CCND1, and CD44 in PC-3 cells. Restoration of miR-141-3p suppresses the expression of the transcription factor KLF9 in PC-3 and accelerates prostate tumorigenesis via targeted binding with its 3'-UTR. Downregulation of KLF9 enhances spheres formation of prostate cancer cells. Our results suggest that miR-141-3p/KLF9 may play an important role in regulating the growth of prostate cancer and is a potential target of prevention and therapy.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China; Department of Urology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, 830001, China.
authors.forename: Jiu-Zhi
authors.initials: JZ
authors.lastname: Li
authors.affiliation: Department of Pediatric Surgery, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Clinical and Medicine Research Center, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, 830001, China.
authors.forename: Hui-Qin
authors.initials: HQ
authors.lastname: Wang
authors.affiliation: Department of Urology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, 830001, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, People's Hospital of Xinjiang Uygur Autonomous Region, Urumqi, Xinjiang, 830001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wen
authors.affiliation: Department of Urology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, 830054, China. Electronic address: yujiewang1122@163.com.
authors.forename: Yu-Jie
authors.initials: YJ
authors.lastname: Wang
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D019938:Cyclin D1 / Q000378:metabolism; D004847:Epithelial Cells / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism; D051741:Kruppel-Like Transcription Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D000071317:Nanog Homeobox Protein / Q000378:metabolism; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D063150:Polycomb Repressive Complex 1 / Q000378:metabolism; D011467:Prostate / Q000378:metabolism; D011471:Prostatic Neoplasms / Q000235:genetics*; D055758:SOX9 Transcription Factor / Q000378:metabolism; D018874:Spheroids, Cellular / Q000378:metabolism
chemical_list: C069077:BMI1 protein, human; C530104:CCND1 protein, human; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; C489676:KLF9 protein, human; D051741:Kruppel-Like Transcription Factors; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; C486780:NANOG protein, human; D000071317:Nanog Homeobox Protein; D050814:Octamer Transcription Factor-3; C494173:POU5F1 protein, human; D055758:SOX9 Transcription Factor; C527932:SOX9 protein, human; D019938:Cyclin D1; D063150:Polycomb Repressive Complex 1
keywords: KLF9; MiR-141-3p; Proliferation; Prostate cancer; Stemness