title: Rapid, cost-effective and accurate quantification of Yucca schidigera Roezl. steroidal saponins using HPLC-ELSD method.
abstract: Yucca GRAS-labelled saponins have been and are increasingly used in food/feed, pharmaceutical or cosmetic industries. Existing techniques presently used for Yucca steroidal saponin quantification remain either inaccurate and misleading or accurate but time consuming and cost prohibitive. The method reported here addresses all of the above challenges. HPLC/ELSD technique is an accurate and reliable method that yields results of appropriate repeatability and reproducibility. This method does not over- or under-estimate levels of steroidal saponins. HPLC/ELSD method does not require each and every pure standard of saponins, to quantify the group of steroidal saponins. The method is a time- and cost-effective technique that is suitable for routine industrial analyses. HPLC/ELSD methods yield a saponin fingerprints specific to the plant species. As the method is capable of distinguishing saponin profiles from taxonomically distant species, it can unravel plant adulteration issues.
authors.affiliation: Naturex SA, Site d'Agroparc BP 1218, 84911 Avignon Cedex 9, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Tenon
authors.affiliation: Naturex SA, Site d'Agroparc BP 1218, 84911 Avignon Cedex 9, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Feuillère
authors.affiliation: Naturex SA, Site d'Agroparc BP 1218, 84911 Avignon Cedex 9, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Roller
authors.affiliation: Naturex SA, Site d'Agroparc BP 1218, 84911 Avignon Cedex 9, France. Electronic address: s.birtic@naturex.com.
authors.forename: Simona
authors.initials: S
authors.lastname: Birtić
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003362:Cost-Benefit Analysis; D010936:Plant Extracts / Q000032:analysis; D012503:Saponins / Q000032:analysis*; D012542:Scattering, Radiation; D027623:Yucca / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012503:Saponins
keywords: Adulteration; HPLC-ELSD; Quantification; Quillaja; Saponin; Steroidal saponin; Yucca; Yucca brevifolia; Yucca schidigera