title: Virus Nanoparticles Mediated Osteogenic Differentiation of Bone Derived Mesenchymal Stem Cells.
abstract: There are few methodologies that allow manipulating a biomaterial surface at nanometer scale, which controllably influence different cellular functions. In this study, virus nanoparticles with different structural features are selected to prepare 2D substrates with defined nanoscale topographies and the cellular responses are investigated. It is demonstrated that the viral nanoparticle based substrates could accelerate and enhance osteogenesis of bone derived mesenchymal stem cells as indicated by the upregulation of osteogenic markers, including bone morphogenetic protein-2, osteocalcin, and osteopontin, at both gene and protein expression levels. Moreover, alkaline phosphatase activity and calcium mineralization, both indicators for a -successful bone formation, are also increased in cells grown on these nanoscale possessed substrates. These discoveries and developments present a new paradigm for nanoscale engineering of a biomaterial surface.
authors.affiliation: Department of Chemistry and Biochemistry University of South Carolina 631 Sumter Street Columbia SC 29208 USA.
authors.forename: Kamolrat
authors.initials: K
authors.lastname: Metavarayuth
authors.affiliation: Department of Chemistry and Biochemistry University of South Carolina 631 Sumter Street Columbia SC 29208 USA.
authors.forename: Pongkwan
authors.initials: P
authors.lastname: Sitasuwan
authors.affiliation: Department of Food and Pharmaceutical Chemistry Faculty of Pharmaceutical Sciences Chulalongkorn University 254 Phayathai Rd., Wangmai Pathumwan Bangkok 10330 Thailand.
authors.forename: Jittima Amie
authors.initials: JA
authors.lastname: Luckanagul
authors.affiliation: Department of Chemistry and Biochemistry University of South Carolina 631 Sumter Street Columbia SC 29208 USA.
authors.forename: Sheng
authors.initials: S
authors.lastname: Feng
authors.affiliation: Department of Chemistry and Biochemistry University of South Carolina 631 Sumter Street Columbia SC 29208 USA.
authors.forename: Qian
authors.initials: Q
authors.lastname: Wang
keywords: biomaterials; mesenchymal stem cells; nanotopography; osteogenesis; virus nanoparticles