title: Development of isotope dilution-liquid chromatography/tandem mass spectrometry for the accurate determination of trans- and cis-vitamin K1isomers in infant formula.
abstract: A method based on isotope dilution-liquid chromatography/tandem mass spectrometry (ID-LC/MS/MS) using a C30 column has been developed for the separate and accurate determination of trans- and cis-vitamin K1in infant formula. Vitamin K1and the deuterium-labeled internal standard eluted at slightly different retention times experiencing different matrix effects, and this possibly resulted in biased measurement. The matrix effect profiles obtained from post-column infusion experiments showed that atmospheric pressure chemical ionization (APCI) was less susceptible to matrix effects near the retention time than electrospray ionization (ESI); therefore, APCI was used in this study. The developed method was validated by measuring fortified samples, and the results agreed with the gravimetric values. Its repeatability and reproducibly were within 2% relative standard deviation. The relative expanded uncertainty was approximately 5%, indicating that the method was of higher-order metrological quality as a reference method.
authors.affiliation: Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
authors.forename: Hyeyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
authors.forename: Joonhee
authors.initials: J
authors.lastname: Lee
authors.affiliation: Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
authors.forename: Kihwan
authors.initials: K
authors.lastname: Choi
authors.affiliation: Korea Research Institute of Standards and Science, 267 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address: byungjoo@kriss.re.kr.
authors.forename: Byungjoo
authors.initials: B
authors.lastname: Kim
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods*; D006801:Humans; D007201:Indicator Dilution Techniques; D007223:Infant; D041943:Infant Formula / Q000032:analysis*; D007536:Isomerism; D053719:Tandem Mass Spectrometry / Q000379:methods*; D010837:Vitamin K 1 / Q000032:analysis*
chemical_list: D010837:Vitamin K 1
keywords: Higher-order reference method; Infant formula; Isotope dilution mass spectrometry; Matrix effects; Trans- and cis-vitamin K(1); Vitamin K(1) (PubChem CID: 5280483)