title: Tracking the Impact of Excisional Cervical Treatment on the Cervix using Biospectroscopy.
abstract: Local excisional treatment for cervical intra-epithelial neoplasia (CIN) is linked to significant adverse sequelae including preterm birth, with cone depth and radicality of treatment correlating to the frequency and severity of adverse events. Attenuated total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy can detect underlying cervical disease more accurately than conventional cytology. The chemical profile of cells pre- and post-treatment may differ as a result of altered biochemical processes due to excision, or treatment of the disease. Since pre-treatment cervical length varies amongst women, the percentage of cervix excised may correlate more accurately to risk than absolute dimensions. We show that treatment for CIN significantly alters the biochemistry of the cervix, compared with women who have not had treatment; this is due to the removal of cervical tissue rather than the removal of the disease. However, the spectra do not seem to correlate to the cone depth or proportion of cervical length excised. Future research should aim to explore the impact of treatment in a larger cohort.
authors.affiliation: Centre for Biophotonics, LEC, Lancaster University, Lancaster, UK.
authors.forename: Diane E
authors.initials: DE
authors.lastname: Halliwell
authors.affiliation: Institute of Reproductive and Developmental Biology, Department of Surgery &Cancer, Faculty of Medicine, Imperial College, London, UK.|West London Gynaecological Cancer Centre, Imperial College NHS Healthcare, London, UK.
authors.forename: Maria
authors.initials: M
authors.lastname: Kyrgiou
authors.affiliation: Institute of Reproductive and Developmental Biology, Department of Surgery &Cancer, Faculty of Medicine, Imperial College, London, UK.|West London Gynaecological Cancer Centre, Imperial College NHS Healthcare, London, UK.
authors.forename: Anita
authors.initials: A
authors.lastname: Mitra
authors.affiliation: Institute of Reproductive and Developmental Biology, Department of Surgery &Cancer, Faculty of Medicine, Imperial College, London, UK.|West London Gynaecological Cancer Centre, Imperial College NHS Healthcare, London, UK.
authors.forename: Ilkka
authors.initials: I
authors.lastname: Kalliala
authors.affiliation: Department of Obstetrics and Gynaecology, University of Ioannina, Ioannina, Greece.
authors.forename: Evangelos
authors.initials: E
authors.lastname: Paraskevaidis
authors.affiliation: St James Hospital, Leeds, West Yorkshire, UK.
authors.forename: Georgios
authors.initials: G
authors.lastname: Theophilou
authors.affiliation: Department of Obstetrics and Gynaecology, Lancashire Teaching Hospitals NHS Trust Foundation, Preston, UK.
authors.forename: Pierre L
authors.initials: PL
authors.lastname: Martin-Hirsch
authors.affiliation: Centre for Biophotonics, LEC, Lancaster University, Lancaster, UK.|School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, UK.
authors.forename: Francis L
authors.initials: FL
authors.lastname: Martin
mesh_terms: D000293:Adolescent; D000328:Adult; D002584:Cervix Uteri / Q000601:surgery*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D017550:Spectroscopy, Fourier Transform Infrared / Q000379:methods*; D002578:Uterine Cervical Dysplasia / Q000175:diagnosis* / Q000601:surgery*