title: Engineering Fungal Nonribosomal Peptide Synthetase-like Enzymes by Heterologous Expression and Domain Swapping.
abstract: A facile genetic methodology in the filamentous fungus Aspergillus nidulans allowed the exchange of various domains in nonribosomal peptide synthase (NRPS)-like enzymes from Aspergillus terreus. The newly generated engineered enzymes are capable of producing compounds with different chemical structures than its parent enzyme in vivo. This work provides insight in the programing of nonribosomal peptide biosynthesis in filamentous fungi.
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , Los Angeles, California 90089, United States.
authors.forename: Johannes W A
authors.identifier: 0000-0001-8558-4655
authors.initials: JW
authors.lastname: van Dijk
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , Los Angeles, California 90089, United States.
authors.forename: Chun-Jun
authors.initials: CJ
authors.lastname: Guo
authors.affiliation: Department of Pharmacology and Pharmaceutical Sciences, University of Southern California, School of Pharmacy , Los Angeles, California 90089, United States.|Department of Chemistry, College of Letters, Arts, and Sciences, University of Southern California , Los Angeles, California 90089, United States.
authors.forename: Clay C C
authors.initials: CC
authors.lastname: Wang
mesh_terms: D001233:Aspergillus nidulans / Q000201:enzymology*; D015394:Molecular Structure; D046908:Peptide Biosynthesis, Nucleic Acid-Independent; D010453:Peptide Synthases / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D015202:Protein Engineering
chemical_list: D010455:Peptides; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase