title: Cellulose Nanofibers Prepared Using the TEMPO/Laccase/O2System.
abstract: The 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO)/laccase/O2system was used to prepare cellulose nanofibers from wood cellulose without requiring any chlorine-containing oxidant. Laccase was degraded by oxidized TEMPO (TEMPO+) formed by laccase-mediated oxidation with O2, which competed with the oxidation of wood cellulose. Thus, large amounts of laccase and TEMPO and a long reaction time were needed to introduce ∼0.6 mmol g-1of C6-carboxylate groups onto wood cellulose. The TEMPO/laccase/O2system underwent one-way reaction from TEMPO to reduced TEMPO through TEMPO+. When the oxidation was applied again to the oxidized wood cellulose following isolation and purification, the C6-carboxylate groups increased to ∼1.1 mmol g-1, which was sufficient to convert the sample to cellulose nanofibers by sonication in water. However, the higher the carboxylate content of the oxidized celluloses, the lower their degree of polymerization.
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab of Biomass-Based Green Fuel & Chemicals, College of Chemical Engineering, Nanjing Forestry University , Nanjing 210037, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab of Biomass-Based Green Fuel & Chemicals, College of Chemical Engineering, Nanjing Forestry University , Nanjing 210037, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Ye
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab of Biomass-Based Green Fuel & Chemicals, College of Chemical Engineering, Nanjing Forestry University , Nanjing 210037, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Liu
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab of Biomass-Based Green Fuel & Chemicals, College of Chemical Engineering, Nanjing Forestry University , Nanjing 210037, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, Jiangsu Key Lab of Biomass-Based Green Fuel & Chemicals, College of Chemical Engineering, Nanjing Forestry University , Nanjing 210037, China.
authors.forename: Yimin
authors.identifier: 0000-0003-2764-1310
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Department of Biomaterials Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo , Tokyo 113-8657, Japan.
authors.forename: Tsuguyuki
authors.initials: T
authors.lastname: Saito
authors.affiliation: Department of Biomaterials Science, Graduate School of Agricultural and Life Sciences, The University of Tokyo , Tokyo 113-8657, Japan.
authors.forename: Akira
authors.identifier: 0000-0001-8095-0441
authors.initials: A
authors.lastname: Isogai
mesh_terms: D002482:Cellulose / Q000737:chemistry*; D003497:Cyclic N-Oxides / Q000378:metabolism*; D042845:Laccase / Q000378:metabolism*; D057139:Nanofibers / Q000737:chemistry*; D010084:Oxidation-Reduction; D010100:Oxygen / Q000378:metabolism*; D058105:Polymerization; D014867:Water / Q000737:chemistry
chemical_list: D003497:Cyclic N-Oxides; D014867:Water; D002482:Cellulose; D042845:Laccase; D010100:Oxygen; C003959:TEMPO