title: Ofloxacin loaded gellan/PVA nanofibers - Synthesis, characterization and evaluation of their gastroretentive/mucoadhesive drug delivery potential.
abstract: The purpose of this investigation is to formulate a gastroretentive sustained drug release system for ofloxacin to improve its retention time, pharmacological activity, bioavailability and therapeutic efficacy in the stomach. Ofloxacin loaded gellan/poly vinyl alcohol (PVA) nanofibers were fabricated using a simple and versatile electrospinning technique. The fabricated nanofibers were evaluated for percent drug encapsulation efficiency and in vitro drug release in simulated gastric medium (pH1.2). The in vitro release profile and kinetic studies for drug indicated the sustained release of ofloxacin from the nanofibers through Fickian diffusion kinetics. The antimicrobial activity of the ofloxacin loaded nanofibers was assessed in comparison to the pure ofloxacin by means of minimal inhibitory concentrations (MIC) against microbial strains of Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa. The optimized ofloxacin loaded gellan/PVA nanofibers displayed biphasic drug release profile with considerable mucoadhesion and gastric retention in the rat's gastric mucosal membrane. Data obtained, suggested that the developed gastroretentive drug delivery can potentially enhance the pharmacological activity of ofloxacin and can also serve as a viable alternative for improving drug bioavailability via oral route.
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
authors.forename: Priya
authors.initials: P
authors.lastname: Vashisth
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
authors.forename: Navdeep
authors.initials: N
authors.lastname: Raghuwanshi
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
authors.forename: Amit Kumar
authors.initials: AK
authors.lastname: Srivastava
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
authors.forename: Harmeet
authors.initials: H
authors.lastname: Singh
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
authors.forename: Hemant
authors.initials: H
authors.lastname: Nagar
authors.affiliation: Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India. Electronic address: vikasfbs@gmail.com.
authors.forename: Vikas
authors.initials: V
authors.lastname: Pruthi
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001419:Bacteria / Q000254:growth & development*; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005753:Gastric Mucosa / Q000276:immunology / Q000378:metabolism; D015242:Ofloxacin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D011135:Polysaccharides, Bacterial / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D011142:Polyvinyl Alcohol / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000890:Anti-Infective Agents; D003692:Delayed-Action Preparations; D011135:Polysaccharides, Bacterial; C048288:gellan gum; D011142:Polyvinyl Alcohol; D015242:Ofloxacin
keywords: Drug delivery; Electrospinning; Gastric retention; Gellan; Nanofibers