title: Inhibitory Effect of Methyl 2-(4'-Methoxy-4'-oxobutanamide) Benzoate from Jerusalem Artichoke (Helianthus tuberosus) on the Inflammatory Paracrine Loop between Macrophages and Adipocytes.
abstract: The interaction between macrophages and adipocytes is known to aggravate inflammation of the adipose tissue, leading to decreased insulin sensitivity. Hence, attenuation of the inflammatory paracrine loop between macrophages and adipocytes is deemed essential to ameliorate insulin resistance and diabetes mellitus type 2. Methyl 2-(4'-methoxy-4'-oxobutanamide) benzoate (compound 1), a newly isolated compound from Jerusalem srtichoke (JA), has not been biologically characterized yet. Here, we investigated whether JA-derived compound 1 attenuates the inflammatory cycle between RAW 264.7 macrophages and 3T3-L1 adipocytes. Compound 1 suppressed the inflammatory response of RAW 264.7 cells to lipopolysaccharide through decreased secretion of IL-1β, IL-6, and TNF-α. Moreover, the mRNA expression of TNF-α, IL-6, IL-1β, MCP-1, and Rantes and MAPK pathway activation in 3T3-L1 adipocytes, incubated in macrophage-conditioned media, were inhibited. These findings suggest an anti-inflammatory effect of a newly extracted compound against adipose tissue inflammation and insulin resistance.
authors.affiliation: School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
authors.forename: Yun Joo
authors.initials: YJ
authors.lastname: Jung
authors.affiliation: School of Food Sciences & Biotechnology, College of Agriculture & Life Sciences, Kyungpook National University , Daegu 41566, Republic of Korea.
authors.forename: Byung Oh
authors.initials: BO
authors.lastname: Kim
authors.affiliation: School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
authors.forename: Jong Hwan
authors.initials: JH
authors.lastname: Kwak
authors.affiliation: School of Pharmacy, Sungkyunkwan University , Suwon 16419, Republic of Korea.
authors.forename: Suhkneung
authors.initials: S
authors.lastname: Pyo
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000276:immunology; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology*; D001565:Benzoates / Q000494:pharmacology*; D006368:Helianthus / Q000737:chemistry*; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008264:Macrophages / Q000187:drug effects* / Q000276:immunology; D051379:Mice; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology
chemical_list: D000893:Anti-Inflammatory Agents; D001565:Benzoates; D053583:Interleukin-1beta; D015850:Interleukin-6; D010936:Plant Extracts; D014409:Tumor Necrosis Factor-alpha
keywords: 3T3-L1; Helianthus tuberosus; Jerusalem artichoke; RAW 264.7; inflammation; insulin resistance; lipopolysaccharides; macrophage-conditioned media; obesity