title: Suppressive effect of microRNA-143 in retinoblastoma.
abstract: OBJECTIVE
  To investigate microRNA-143 expression and effect on suppression of retinoblastoma (RB) cells.
  
  
  METHODS
  The expression of microRNA-143 was investigated and compared in normal human retina tissue samples and in RB cell lines of Y79 and Weri1. The microRNA-143 mimics were transfected into the RB cell lines separately, and its effect on RB cell lines was detected using reverse-transcription quantitative polymerase chain reaction and Western blotting methods.
  
  
  RESULTS
  The microRNA-143 expression was significantly suppressed in RB cell lines. Overexpression of microRNA-143 significantly lowered cell viability and invasion of the RB cell lines, and increased the number of apoptotic cells. Meanwhile, the Bax expression was up-regulated and much higher in the microRNA-143 mimics transfected group than that in the negative control and the microRNA-143 inhibitor groups.
  
  
  CONCLUSIONS
  MicroRNA-143 exhibits suppressive effects in RB. The current study provides the perspective of a potential therapeutic treatment for RB.
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Yan'an University, Yan'an 716000, Shaanxi Province, China.
authors.forename: Li-Lun
authors.initials: LL
authors.lastname: Wang
authors.affiliation: Department of Oncology, Affiliated Hospital of Yan'an University, Yan'an 716000, Shaanxi Province, China.
authors.forename: Hai-Feng
authors.initials: HF
authors.lastname: Hu
authors.affiliation: Department of Ophthalmology, Affiliated Hospital of Yan'an University, Yan'an 716000, Shaanxi Province, China.
authors.forename: Yan-Qin
authors.initials: YQ
authors.lastname: Feng
keywords: apoptosis; invasion; microRNA-143; proliferation; retinoblastoma