title: Cloning and Expression of a Subfamily 1.4 Lipase fromBacillus licheniformisIBRL-CHS2.
abstract: We report on the cloning of the lipase gene fromBacillus licheniformisIBRL-CHS2 and the expression of the recombinant lipase. DNA sequencing analysis of the cloned lipase gene showed that it shares 99% identity with the lipase gene fromB. licheniformisATCC 14580 and belongs to subfamily 1.4 of true lipases based on amino acid sequence alignment of variousBacilluslipases. The 612 bp lipase gene was then cloned into the pET-15b(+) expression vector and the construct was transformed intoE. coliBL21 (DE3) for bulk expression of the lipase. Expression was analysed by SDS-PAGE where the lipase was found to have a molecular weight of about 23 kDa.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Nidyaletchmy Subba
authors.initials: NS
authors.lastname: Reddy
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Rashidah Abdul
authors.initials: RA
authors.lastname: Rahim
authors.affiliation: Industrial Biotechnology Research Laboratory, School of Biological Sciences, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Darah
authors.initials: D
authors.lastname: Ibrahim
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: K Sudesh
authors.initials: KS
authors.lastname: Kumar
keywords: Bacillus licheniformis; Cloning; Expression; Lipase; Subfamily 1.4 Lipase