title: Microtiter-Format Assays for HBV Antigen Quantitation in Nonclinical Applications.
abstract: Measurement of secreted HBV antigens in cell culture is an important endpoint in many experimental settings. Here we describe convenient and inexpensive protocols for 96-well format sandwich ELISA assays for this purpose, in which there are many options for customization of antibodies used and other parameters. These protocols can be adapted to use for animal serum samples, compound library screening, and other purposes.
authors.affiliation: Baruch S. Blumberg Institute, 3805 Old Easton Road, Doylestown, PA, 18902, USA.
authors.forename: Cally D
authors.initials: CD
authors.lastname: Goddard
authors.affiliation: Baruch S. Blumberg Institute, 3805 Old Easton Road, Doylestown, PA, 18902, USA.
authors.forename: Lale
authors.initials: L
authors.lastname: Bildrici-Ertekin
authors.affiliation: Arbutus Biopharma, Inc., 3805 Old Easton Road, Doylestown, PA, 18902, USA.
authors.forename: Xiaohe
authors.initials: X
authors.lastname: Wang
authors.affiliation: Arbutus Biopharma, Inc., 3805 Old Easton Road, Doylestown, PA, 18902, USA. acuconati@arbutusbio.com.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cuconati
mesh_terms: D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B / Q000276:immunology; D006511:Hepatitis B Antigens / Q000276:immunology*; D006514:Hepatitis B Surface Antigens / Q000276:immunology; D006513:Hepatitis B e Antigens / Q000276:immunology; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D006511:Hepatitis B Antigens; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens
keywords: ELISA; HBV antigen; HBeAg; HBsAg; Screening; Z-factor