title: DNA-Sensing and Nuclease Gene Expressions as Markers for Colorectal Cancer Progression.
abstract: OBJECTIVE
  Oncogene-driven stress-related DNA damage has been observed in lesions of colon cancer. Furthermore, DNA sensors and nucleases are stimulated during active DNA damage and replication. However, their changes and influences with respect to cancer remain largely unknown.
  
  
  METHODS
  The gene expression levels of cGAS, IFI16, STING, TBK1, IFNB1, TREX1, SAMHD1, RNASEH2A, RNASEH2B, and RNASEH2C were examined in the paired colorectal cancer and adjacent normal part tissues of 53 patients. Their associations with the clinical stages of cancer were then analyzed.
  
  
  RESULTS
  All cytosolic DNA-sensing and nuclease-related genes except cGAS, RNASEH2A, and RNASEH2B showed lower mRNA expressions in the colorectal tumor tissues. Moreover, cGAS upregulation was found to be associated with early-stage colorectal cancers, while higher expressions of RNASEH2B, RNASEH2C, and SAMHD1 correlated with metastasis. RNASEH2C knockdown in a colon cancer cell line impaired cell migration, and analysis of the cancer RNA-seq data from The Cancer Genome Atlas (TCGA) database revealed a negative correlation between RNASEH2C expression and E-cadherin levels.
  
  
  CONCLUSIONS
  In contrast to DNA-sensing events in viral infections or autoimmunity, cGAS-STING-IFNB signaling is disrupted in colorectal cancer. The expression levels of cGAS, RNASEH2B, RNASEH2C, and SAMHD1 could be prognostic markers of colorectal cancer.
authors.affiliation: Department of Laboratory Medicine, China Medical University Hospital, Taichung, Taiwan.
authors.forename: Chin-An
authors.initials: CA
authors.lastname: Yang
authors.forename: Hsi-Yuan
authors.initials: HY
authors.lastname: Huang
authors.forename: Ya-Sian
authors.initials: YS
authors.lastname: Chang
authors.forename: Chia-Li
authors.initials: CL
authors.lastname: Lin
authors.forename: I-Lu
authors.initials: IL
authors.lastname: Lai
authors.forename: Jan-Gowth
authors.initials: JG
authors.lastname: Chang
mesh_terms: D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics / Q000473:pathology*; D004247:DNA / Q000378:metabolism*; D018450:Disease Progression; D004720:Endonucleases / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged
chemical_list: D014408:Biomarkers, Tumor; D004247:DNA; D004720:Endonucleases