title: Discovery of a novel accessory protein NS7a encoded by porcine deltacoronavirus.
abstract: Porcine deltacoronavirus (PDCoV) is an emerging swine enteric coronavirus. Bioinformatics predicts that PDCoV encodes two accessory proteins (NS6 and NS7), the species-specific proteins for coronavirus. In this study, four mAbs against the predicted NS7 were prepared by using the purified recombinant NS7 protein. Indirect immunofluorescence assay demonstrated that all mAbs recognized cells transfected with an NS7 expression construct or infected with PDCoV. Western blot showed that NS7-specific mAbs recognized an additional protein band of about 12 kDa from PDCoV-infected cell lysates but not from cells with the ectopic expression of NS7. Detailed analysis suggested that this additional protein band represented a novel accessory protein, termed NS7a, a 100 amino acid polypeptide identical to the 3' end of NS7. Moreover, NS7a is encoded by a separate subgenomic mRNA with a non-canonical transcription regulatory sequence. In summary, our results identified a third accessory protein encoded by PDCoV, which will enhance our understanding of PDCoV.
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Puxian
authors.initials: P
authors.lastname: Fang
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Liurong
authors.initials: L
authors.lastname: Fang
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Hong
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Xiaorong
authors.initials: X
authors.lastname: Liu
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Dong
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Panpan
authors.initials: P
authors.lastname: Ma
authors.affiliation: Department of Immunology and Aetiology, College of Basic Medicine, Hubei University of Chinese Medicine, Wuhan 430065, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bi
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Dang
authors.initials: D
authors.lastname: Wang
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Wuhan 430070, PR China.|State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Xiao
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology; D001483:Base Sequence; D017124:Conserved Sequence; D017934:Coronavirus / Q000235:genetics / Q000378:metabolism*; D019084:Fluorescent Antibody Technique, Indirect; D005761:Gastroenteritis, Transmissible, of Swine / Q000821:virology*; D009154:Mutation; D012333:RNA, Messenger / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D013552:Swine; D014162:Transfection; D054334:Viral Regulatory and Accessory Proteins / Q000235:genetics / Q000276:immunology / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D012333:RNA, Messenger; D011994:Recombinant Proteins; D054334:Viral Regulatory and Accessory Proteins