title: Three-Dimensional Arrangement of Human Bone Marrow Microvessels Revealed by Immunohistology in Undecalcified Sections.
abstract: The arrangement of microvessels in human bone marrow is so far unknown. We combined monoclonal antibodies against CD34 and against CD141 to visualise all microvessel endothelia in 21 serial sections of about 1 cm2 size derived from a human iliac crest. The specimen was not decalcified and embedded in Technovit® 9100. In different regions of interest, the microvasculature was reconstructed in three dimensions using automatic methods. The three-dimensional models were subject to a rigid semiautomatic and manual quality control. In iliac crest bone marrow, the adipose tissue harbours irregularly distributed haematopoietic areas. These are fed by networks of large sinuses, which are loosely connected to networks of small capillaries prevailing in areas of pure adipose tissue. Our findings are compatible with the hypothesis that capillaries and sinuses in human iliac crest bone marrow are partially arranged in parallel.
authors.affiliation: Department of Immunobiology, Institute of Anatomy and Cell Biology, Universität Marburg, Marburg, Germany.
authors.forename: Birte S
authors.initials: BS
authors.lastname: Steiniger
authors.affiliation: Dental Clinics, Histology Laboratory, Universität Marburg, Marburg, Germany.
authors.forename: Vitus
authors.initials: V
authors.lastname: Stachniss
authors.affiliation: Department of Immunobiology, Institute of Anatomy and Cell Biology, Universität Marburg, Marburg, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Wilhelmi
authors.affiliation: Department of Immunobiology, Institute of Anatomy and Cell Biology, Universität Marburg, Marburg, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Seiler
authors.affiliation: Department of Immunobiology, Institute of Anatomy and Cell Biology, Universität Marburg, Marburg, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Lampp
authors.affiliation: Department of Oral and Maxillofacial Surgery, Marburg University Hospital, Marburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Neff
authors.affiliation: Visual Computing, Institute of Computer Science, Universität Bayreuth, Bayreuth, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Guthe
authors.affiliation: Visual Computing, Institute of Computer Science, Universität Bayreuth, Bayreuth, Germany.
authors.forename: Oleg
authors.initials: O
authors.lastname: Lobachev
mesh_terms: D000273:Adipose Tissue / Q000098:blood supply* / Q000378:metabolism; D001853:Bone Marrow / Q000098:blood supply* / Q000378:metabolism; D002196:Capillaries; D005260:Female; D006801:Humans; D007085:Ilium / Q000098:blood supply* / Q000378:metabolism; D021621:Imaging, Three-Dimensional; D007150:Immunohistochemistry; D008297:Male