title: Molecular and Serological Survey of Selected Viruses in Free-Ranging Wild Ruminants in Iran.
abstract: A molecular and serological survey of selected viruses in free-ranging wild ruminants was conducted in 13 different districts in Iran. Samples were collected from 64 small wild ruminants belonging to four different species including 25 Mouflon (Ovis orientalis), 22 wild goat (Capra aegagrus), nine Indian gazelle (Gazella bennettii) and eight Goitered gazelle (Gazella subgutturosa) during the national survey for wildlife diseases in Iran. Serum samples were evaluated using serologic antibody tests for Peste de petits ruminants virus (PPRV), Pestiviruses [Border Disease virus (BVD) and Bovine Viral Diarrhoea virus (BVDV)], Bluetongue virus (BTV), Bovine herpesvirus type 1 (BHV-1), and Parainfluenza type 3 (PI3). Sera were also ELISA tested for Pestivirus antigen. Tissue samples including spleen, liver, lung, tonsils, mesenteric and mediastinal lymph nodes and white blood cells (WBCs) were tested using polymerase chain reaction (PCR) for PPRV, Foot and Mouth Disease virus (FMDV), Pestivirus, BTV, Ovine herpesvirus type 2 (OvHV-2) and BHV-1. Serologic tests were positive for antibodies against PPRV (17%), Pestiviruses (2%) and BTV (2%). No antibodies were detected for BHV-1 or PI3, and no Pestivirus antigen was detected. PCR results were positive for PPRV (7.8%), FMDV (11%), BTV (3%), OvHV-2 (31%) and BHV-1 (1.5%). None of the samples were positive for Pestiviruses.
authors.affiliation: School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia.
authors.forename: Farhid
authors.initials: F
authors.lastname: Hemmatzadeh
authors.affiliation: School of Animal and Veterinary Sciences, The University of Adelaide, Adelaide, Australia.|School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, Australia.
authors.forename: Wayne
authors.initials: W
authors.lastname: Boardman
authors.affiliation: DVM graduate, Faculty of Veterinary Medicine, The University of Tehran, Tehran, Iran.
authors.forename: Arezo
authors.initials: A
authors.lastname: Alinejad
authors.affiliation: Faculty of Agriculture, Islamic Azad University, Shahrekord branch, Shahrekord, Iran.
authors.forename: Azar
authors.initials: A
authors.lastname: Hematzade
authors.affiliation: Iran Department of Environment, Tehran, Iran.
authors.forename: Majid Kharazian
authors.initials: MK
authors.lastname: Moghadam
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood*; D007492:Iran; D016133:Polymerase Chain Reaction / Q000379:methods*; D012418:Ruminants / Q000097:blood* / Q000821:virology*; D014777:Virus Diseases / Q000097:blood / Q000235:genetics / Q000662:veterinary; D014780:Viruses / Q000235:genetics*
chemical_list: D000914:Antibodies, Viral