title: Comparative Study on the Characteristics of Weissella cibaria CMU and Probiotic Strains for Oral Care.
abstract: Probiotics have been demonstrated as a new paradigm to substitute antibiotic treatment for dental caries, gingivitis, and chronic periodontitis. The present work was conducted to compare the characteristics of oral care probiotics:Weissella cibariaCMU (Chonnam Medical University) and four commercial probiotic strains. Survival rates under poor oral conditions, acid production, hydrogen peroxide production, as well as inhibition of biofilm formation, coaggregation, antibacterial activity, and inhibition of volatile sulfur compounds were evaluated. The viability ofW. cibariaCMU was not affected by treatment of 100 mg/L lysozyme for 90 min and 1 mM hydrogen peroxide for 6 h. Interestingly,W. cibariaproduced less acid and more hydrogen peroxide than the other four probiotics.W. cibariainhibited biofilm formation byStreptococcus mutansat lower concentrations (S. mutans/CMU = 8) and efficiently coaggregated withFusobacterium nucleatum.W. cibariaCMU and two commercial probiotics, includingLactobacillus salivariusandLactobacillus reuteri, showed high antibacterial activities (>97%) against cariogens (S. mutansandStreptococcus sobrinus), and against periodontopathogens (F. nucleatumandPorphyromonas gingivalis). All of the lactic acid bacterial strains in this study significantly reduced levels of hydrogen sulfide and methyl mercaptan produced byF. nucleatumandP. gingivalis(p< 0.05). These results suggest thatW. cibariaCMU is applicable as an oral care probiotic.
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute (KFRI), 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13539, Korea. Jang.Hye-jin@kfri.re.kr.
authors.forename: Hye-Jin
authors.initials: HJ
authors.lastname: Jang
authors.affiliation: Oradentics Co., Ltd., 3F KM Building 23, Teheran-ro 77-gil, Gangnam-gu, Seoul 06157, Korea. jieenkang@oradentics.com.
authors.forename: Mi-Sun
authors.initials: MS
authors.lastname: Kang
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute (KFRI), 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13539, Korea. sunghunyi@kfri.re.kr.
authors.forename: Sung-Hun
authors.initials: SH
authors.lastname: Yi
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute (KFRI), 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13539, Korea. Hong.Ji-young@kfri.re.kr.
authors.forename: Ji-Young
authors.initials: JY
authors.lastname: Hong
authors.affiliation: Division of Strategic Food Research, Korea Food Research Institute (KFRI), 1201-62 Anyangpangyo-ro, Bundang-gu, Seongnam-si, Gyeonggi-do 13539, Korea. sphong@kfri.re.kr.
authors.forename: Sang-Pil
authors.initials: SP
authors.lastname: Hong
mesh_terms: D018441:Biofilms; D003731:Dental Caries / Q000517:prevention & control*; D005673:Fusobacterium / Q000502:physiology; D006861:Hydrogen Peroxide / Q000378:metabolism; D007778:Lactobacillus / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D019936:Probiotics; D013295:Streptococcus mutans / Q000502:physiology; D013457:Sulfur Compounds / Q000378:metabolism; D058836:Weissella / Q000378:metabolism*
chemical_list: D013457:Sulfur Compounds; D006861:Hydrogen Peroxide; D009113:Muramidase
keywords: Weissella cibaria; antibacterial; biofilm; hydrogen peroxide; oral care; probiotics; volatile sulfur compound