title: Maximization of cell growth and lipid production of freshwater microalga Chlorella vulgaris by enrichment technique for biodiesel production.
abstract: Chlorella vulgaris was cultivated under limitation and starvation and under controlled conditions using different concentrations of nitrate (NaNO3) and phosphate (K2HPO4and KH2PO4) chemicals in modified Bold basal medium (BBM). The biomass and lipid production responses to different media were examined in terms of optical density, cell density, dry biomass, and lipid productivity. In the 12-day batch culture period, the highest biomass productivity obtained was 72.083 mg L-1day-1under BBM - NcontrolPlimitedcondition. The highest lipid content, lipid concentration, and lipid productivity obtained were 53.202 %, 287.291 mg/L, and 23.449 mg L-1day-1under BBM - NControlPDeprivationcondition, respectively. Nitrogen had a major effect in the biomass concentration of C. vulgaris, while no significant effect was found for phosphorus. Nitrogen and phosphorus starvation was found to be the strategy affecting the lipid accumulation and affected the lipid composition of C. vulgaris cultures.
authors.affiliation: Biology Department, The Hong Kong Baptist University, Kowloon, Hong Kong.|Center for Research in Environmental Science, The Open University of Hong Kong, Hong Kong, China.
authors.forename: Y K
authors.initials: YK
authors.lastname: Wong
authors.affiliation: Center for Research in Environmental Science, The Open University of Hong Kong, Hong Kong, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Ho
authors.affiliation: Center for Research in Environmental Science, The Open University of Hong Kong, Hong Kong, China.
authors.forename: K C
authors.initials: KC
authors.lastname: Ho
authors.affiliation: Biology Department, The Hong Kong Baptist University, Kowloon, Hong Kong.
authors.forename: H M
authors.initials: HM
authors.lastname: Leung
authors.affiliation: Biology Department, The Hong Kong Baptist University, Kowloon, Hong Kong. kklyung@hkbu.edu.hk.
authors.forename: K K L
authors.initials: KK
authors.lastname: Yung
mesh_terms: D056804:Biofuels; D018533:Biomass; D002708:Chlorella; D048408:Chlorella vulgaris; D005618:Fresh Water; D008055:Lipids / Q000737:chemistry; D058086:Microalgae
chemical_list: D056804:Biofuels; D008055:Lipids
keywords: Algal cultivation; Chlorella vulgaris; Culture medium; Lipid production