title: Differential mobility spectrometry combined with multiple ion monitoring for bioanalysis of disulfide-bonded peptides with inefficient collision-induced dissociation fragmentation.
abstract: OBJECTIVE
  It is challenging to develop a multiple reaction monitoring (MRM) method for some disulfide-bonded peptides with inefficient collision-induced dissociation fragmentation. This study describes a new methodology using differential mobility spectrometry (DMS) combined with multiple ion monitoring (MIM) to enhance bioanalytical sensitivity for sunflower trypsin inhibitor.
  
  
  RESULTS
  By combining DMS with MIM to monitor the intact precursor ion in Q1 and Q3 MS analyzers, a lower limit of quantitation at 0.125 ng/ml was achieved to quantify sunflower trypsin inhibitor in rat plasma, representing a 40-fold sensitivity improvement over MIM without DMS.
  
  
  CONCLUSIONS
  DMS coupled with MIM method provides triple quadrupole MS users an effective means to overcome challenges in analyzing disulfide-bonded peptides or other analytes that do not have useful collision-induced dissociation fragment ions for MRM analysis.
authors.affiliation: Application Support North America, Sciex, 500 Old Connecticut Path, Framingham, MA 01701, USA.
authors.forename: Yuan-Qing
authors.initials: YQ
authors.lastname: Xia
authors.affiliation: Pharmaceutical Candidate Optimization, Bristol-Myers Squibb Company, Princeton, NJ 08543, USA.
authors.forename: Eugene
authors.initials: E
authors.lastname: Ciccimaro
authors.affiliation: Analytical & Bioanalytical Operations, Bristol-Myers Squibb Company, Princeton, NJ 08543, USA.
authors.forename: Naiyu
authors.initials: N
authors.lastname: Zheng
authors.affiliation: Pharmaceutical Candidate Optimization, Bristol-Myers Squibb Company, Princeton, NJ 08543, USA.
authors.forename: Mingshe
authors.initials: M
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004220:Disulfides / Q000737:chemistry*; D007477:Ions / Q000737:chemistry; D057230:Limit of Detection; D013058:Mass Spectrometry; D010456:Peptides, Cyclic / Q000097:blood*; D051381:Rats
chemical_list: D004220:Disulfides; D007477:Ions; D010456:Peptides, Cyclic; C448501:SFTI-1 peptide, sunflower
keywords: differential mobility spectrometry; disulfide-bonded peptides; multiple ion monitoring