title: Proteomic Analyses of Agkistrodon contortrix contortrix Venom Using 2D Electrophoresis and MS Techniques.
abstract: Snake venom is a complex mixture of proteins and peptides which in the Viperidae is mainly hemotoxic. The diversity of these components causes the venom to be an extremely interesting object of study. Discovered components can be used in search for new pharmaceuticals used primarily in the treatment of diseases of the cardiovascular system. In order to determine the protein composition of the southern copperhead venom, we have used high resolution two dimensional electrophoresis and MALDI ToF/ToF MS-based identification. We have identified 10 groups of proteins present in the venom, of which phospholipase A₂ and metalloprotease and serine proteases constitute the largest groups. For the first time presence of 5'-nucleotidase in venom was found in this group of snakes. Three peptides present in the venom were also identified. Two of them as bradykinin-potentiating agents and one as an inhibitor.
authors.affiliation: Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. bocian@prz.edu.pl.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Bocian
authors.affiliation: Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. murbanik92@wp.pl.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Urbanik
authors.affiliation: Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. knr.hus@gmail.com.
authors.forename: Konrad
authors.initials: K
authors.lastname: Hus
authors.affiliation: Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. andrzej.lyskowski@prz.edu.pl.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Łyskowski
authors.affiliation: Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic. petrillav@gmail.com.|Zoological Department, Zoological Garden Košice, Široká 31, 040 06 Košice-Kavečany, Slovac Republic. petrillav@gmail.com.
authors.forename: Vladimír
authors.initials: V
authors.lastname: Petrilla
authors.affiliation: Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic. zuzka.kravcova@gmail.com.
authors.forename: Zuzana
authors.initials: Z
authors.lastname: Andrejčáková
authors.affiliation: Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic. monika.petrillova@uvlf.sk.
authors.forename: Monika
authors.initials: M
authors.lastname: Petrillová
authors.affiliation: Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland. jlegath@prz.edu.pl.|Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovak Republic. jlegath@prz.edu.pl.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Legáth
mesh_terms: D003435:Crotalid Venoms / Q000737:chemistry*; D015180:Electrophoresis, Gel, Two-Dimensional; D010455:Peptides / Q000032:analysis*; D040901:Proteomics; D030162:Reptilian Proteins / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: C031442:Agkistrodon venoms; D003435:Crotalid Venoms; D010455:Peptides; D030162:Reptilian Proteins
keywords: Agkistrodon contortrix contortrix; proteomics; southern copperhead; venom