title: [Role of Autophagy in the Radiosensitivity of Human Lung Adenocarcinoma A549 Cells].
abstract: BACKGROUND
  Radiotherapy is an important treatment for lung cancer. The poor prognosis of lung cancer is largely caused by the high recurrence rate and metastasis of the tumor. Autophagy, which can be induced by radiotherapy, might be associated with DNA repair. The aim of this study is to investigate whether activating autophagy using rapamycin can enhance the radiosensitivity of lung cancer cells and clarify the association of autophagy with DNA repair.
  
  
  METHODS
  The human adenocarcinoma A549 cell line was selected as the experimental subject. The specimens were divided into three groups: control (N), radiation (R), and Rapamycin and radiation (R+RAPA). The protein levels of γ-H2AX, Rad51, Ku70/Ku80, p62, and LC3 were determined by Western blot. Autophagosome was observed under a transmission electron microscope, and SF was determined by colony formation assay.
  
  
  RESULTS
  Compared with group R, the activity of autophagy and the protein expression levels of Rad51 and Ku70/80 were remarkably increased in group R+RAPA.
  
  
  CONCLUSIONS
  The radiosensitivity of lung cancer can be promoted by activating autophagy via treatment with Rapamycin, and the process may be associated with DNA repair. .
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Liyao
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Respirology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Zhong
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D000072283:A549 Cells; D000230:Adenocarcinoma / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology / Q000532:radiotherapy*; D000077192:Adenocarcinoma of Lung; D001343:Autophagy / Q000528:radiation effects*; D004260:DNA Repair; D005720:Gamma Rays; D006801:Humans; D000072200:Ku Autoantigen / Q000235:genetics / Q000378:metabolism; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism / Q000503:physiopathology / Q000532:radiotherapy*; D051135:Rad51 Recombinase / Q000235:genetics / Q000378:metabolism; D011836:Radiation Tolerance
chemical_list: D051135:Rad51 Recombinase; D000072200:Ku Autoantigen