title: Anti-asthmatic and anxiolytic effects ofHerissantia tiubae, a Brazilian medicinal plant.
abstract: Herissantia tiubae(HtE) is a Brazilian plant used in folk medicine to treat inflammatory diseases. Our aim was to determine whether the HtE has anti-inflammatory and anxiolytic effects in a murine model of asthma. Ovalbumin (OVA)-sensitized BALB/c mice were treated with HtE (50, 100, or 200 mg/kg) or dexamethasone before each OVA challenge. After the last challenge, animals were subjected to anxiety tests and respiratory measurements. Following euthanasia, we quantified immune cells in the bronchoalveolar lavage (BAL), serum IgE titer and cytokine levels, cellular infiltration and mucus content in the lung tissues, and cellular composition of the mediastinal lymph nodes. OVA challenge in sensitized animals caused: (1) reduction of mean respiratory and dominant respiratory rate (from 398 ± 12 to 286 ± 20 cicles per minute (cpm) and from 320 ± 14 to 162 ± 15 cpm, respectively); (2) increase in behavioral markers of anxiety tests; (3) substantial pro-inflammatory effects, including rise in OVA-specific IgE titer (from 0 to 1:2048) and these inflammatory effect diminished the titer to 1:512 after HtE treatment; rise in plasma IL-13 (from 13 ng/mL in saline to 227 ng/mL in OVA and HtE treatment restored to 1.29 ng/mL; rise in total BAL cell count (from 0.742 cells/mL in saline to 11.77 cells/mL in OVA), with prominent eosinophilia.H. tiubaeextract affected respiratory parameters similarly to aminophylline, behavioral changes comparable to diazepam, and inflammation being as efficient as dexamethasone.H. tiubaeextract (HtE) possesses both anti-inflammatory and anxiolytic properties in the murine model of asthma.
authors.affiliation: Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Talissa
authors.initials: T
authors.lastname: Mozzini Monteiro
authors.affiliation: Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Hermann
authors.initials: H
authors.lastname: Ferrera Costa
authors.affiliation: Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Giciane
authors.initials: G
authors.lastname: Carvalho Vieira
authors.affiliation: Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Paula Regina
authors.initials: PR
authors.lastname: Rodrigues Salgado
authors.affiliation: Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Mirian Graciela
authors.initials: MG
authors.lastname: da Silva Stiebbe Salvadori
authors.affiliation: Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Reinaldo Nobrega
authors.initials: RN
authors.lastname: de Almeida
authors.affiliation: Department of Pharmacy Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Maria
authors.initials: M
authors.lastname: de Fatima Vanderlei de Souza
authors.affiliation: Department of Pharmacy Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Wemerson
authors.initials: W
authors.lastname: Neves Matias
authors.affiliation: Department of Biotechnology, Center of Biotechnology Federal University of Paraiba João Pessoa Paraiba Brazil.
authors.forename: Valdir
authors.initials: V
authors.lastname: Andrade Braga
authors.affiliation: School of Biomedical Sciences and Pharmacy University of Newcastle Newcastle New South Wales Australia.
authors.forename: Eugene
authors.initials: E
authors.lastname: Nalivaiko
authors.affiliation: Laboratory of Immunopharmacology, Department of Physiology and Pathology Federal University of Paraíba João Pessoa Paraíba 58051-970 Brazil.
authors.forename: Marcia Regina
authors.initials: MR
authors.lastname: Piuvezam
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000627:therapeutic use*; D018927:Anti-Asthmatic Agents / Q000627:therapeutic use*; D001007:Anxiety / Q000628:therapy; D001249:Asthma / Q000628:therapy*; D001938:Brazil; D001992:Bronchoalveolar Lavage Fluid; D006896:Hydroxyethylrutoside; D051379:Mice; D010047:Ovalbumin; D010946:Plants, Medicinal
chemical_list: D014151:Anti-Anxiety Agents; D018927:Anti-Asthmatic Agents; D006896:Hydroxyethylrutoside; D010047:Ovalbumin
keywords: Anxiety; Herissantia tiubae; OVA‐sensitized mice; asthma; psychoimmunology