title: Incorporation of silver and strontium in hydroxyapatite coating on titanium surface for enhanced antibacterial and biological properties.
abstract: Implant-related infection in primary total joint prostheses has attracted considerable research attention. As a measure to improve the antimicrobial properties of implant materials, silver (Ag) was incorporated into calcium phosphate (CaP) coatings on Titanium (Ti) via a hydrothermal method. Further, strontium (Sr) was added as a binary dopant to reduce the cytotoxicity of Ag in the coatings. Results showed that the CaP coatings were uniformly deposited on Ti with enhanced hydrophilicity and nanoscale surface roughness. Moreover, cell adhesion, proliferation, and differentiation were improved after the CaP coating deposition. The antibacterial properties of the coatings were distinctly improved by the incorporation of Ag, but the cell proliferation and differentiation were significantly decreased. Owing to the incorporation of Sr, the Ag-CaP coatings were able to effectively counteract the negative effects of Ag while maintaining good antibacterial properties. In summary, hydrothermally deposited CaP coatings doped with Ag and Sr exhibit excellent biocompatibility and antimicrobial activity. Thus, such co-doped CaP coatings have considerable potential for orthopaedic implant modification.
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Geng
authors.affiliation: School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China.
authors.forename: Renfeng
authors.initials: R
authors.lastname: Wang
authors.affiliation: Department of Spinal Surgery, Liuzhou Worker's Hospital, Liuzhou 545001, China. Electronic address: doctorzhuo@139.com.
authors.forename: Xianglong
authors.initials: X
authors.lastname: Zhuo
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Laboratory of Composite and Functional Materials, Tianjin 300072, China. Electronic address: zyli@tju.edu.cn.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Orthopedics Research Center, Peking University Shenzhen Hospital, Shenzhen 518036, China.
authors.forename: Yongcan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ma
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Zhenduo
authors.initials: Z
authors.lastname: Cui
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Laboratory of Composite and Functional Materials, Tianjin 300072, China.
authors.forename: Shengli
authors.initials: S
authors.lastname: Zhu
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China.
authors.forename: Yanqin
authors.initials: Y
authors.lastname: Liang
authors.affiliation: School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China.
authors.forename: Yunde
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China.
authors.forename: Huijing
authors.initials: H
authors.lastname: Bao
authors.affiliation: School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China.
authors.forename: Qianyu
authors.initials: Q
authors.lastname: Huo
authors.affiliation: School of Laboratory Medicine, Tianjin Medical University, Tianjin 300072, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Liu
authors.affiliation: School of Materials Science and Engineering, Tianjin University, Tianjin 300072, China; Tianjin Key Laboratory of Composite and Functional Materials, Tianjin 300072, China. Electronic address: xjyang@tju.edu.cn.
authors.forename: Xianjin
authors.initials: X
authors.lastname: Yang
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000494:pharmacology; D017886:Durapatite / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects / Q000648:ultrastructure; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D010012:Osteogenesis / Q000187:drug effects / Q000235:genetics; D012834:Silver / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013211:Staphylococcus aureus / Q000187:drug effects / Q000648:ultrastructure; D013324:Strontium / Q000494:pharmacology*; D013499:Surface Properties; D014025:Titanium / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D012834:Silver; D017886:Durapatite; D014025:Titanium; D013324:Strontium
keywords: Antibacterial property; Bioactivity; Hydroxyapatite coating; Silver; Strontium