title: Nitinol-Based Nanotubular Arrays with Controlled Diameters Upregulate Human Vascular Cell ECM Production.
abstract: Current approaches to reducing restenosis do not balance the reduction of vascular smooth muscle cell proliferation with the increase in the healing of the endothelium. Building on our previous work, we present our study on the effects of Nitinol-based nanotubular coatings with different nanotube diameters on the reduction of restenosis. Here, we demonstrate that the nanotubular coatings reduced primary human aortic smooth muscle cell (HASMC) proliferation and increased the migration (by more than 4 times), collagen (by 2-3 times per cell) and elastin (by 5-8 times per cell) production of primary human aortic endothelial cells (HAEC). Furthermore, a significant increase in elastin and soluble collagen production of HAEC was observed with an increase in nanotube diameter. Our findings suggest that nanotubes-coated Nitinol may provide a surface conducive for HAEC reendothelialization while reducing the proliferation of HASMC.
authors.affiliation: Department of Bioengineering and Therapeutic Sciences and The UC Berkeley-UCSF Graduate Group in Bioengineering, University of California-San Francisco, San Francisco, California 94158, United States.
authors.forename: Phin P
authors.initials: PP
authors.lastname: Lee
authors.affiliation: Department of Bioengineering and Therapeutic Sciences and The UC Berkeley-UCSF Graduate Group in Bioengineering, University of California-San Francisco, San Francisco, California 94158, United States.
authors.forename: Tejal A
authors.initials: TA
authors.lastname: Desai
keywords: collagen and elastin production; nanotube diameter; nitinol; primary human aortic endothelial and smooth muscle cells; restenosis