title: Ebselen Preserves Tissue-Engineered Cell Sheets and their Stem Cells in Hypothermic Conditions.
abstract: Clinical trials have been performed using autologous tissue-engineered epithelial cell sheets for corneal regenerative medicine. To improve stem cell-based therapy for convenient clinical practice, new techniques are required for preserving reconstructed tissues and their stem/progenitor cells until they are ready for use. In the present study, we screened potential preservative agents and developed a novel medium for preserving the cell sheets and their stem/progenitor cells; the effects were evaluated with a luciferase-based viability assay. Nrf2 activators, specifically ebselen, could maintain high ATP levels during preservation. Ebselen also showed a strong influence on maintenance of the viability, morphology, and stem cell function of the cell sheets preserved under hypothermia by protecting them from reactive oxygen species-induced damage. Furthermore, ebselen drastically improved the preservation performance of human cornea tissues and their stem cells. Therefore, ebselen shows good potential as a useful preservation agent in regenerative medicine as well as in cornea transplantation.
authors.affiliation: Department of Ophthalmology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Katori
authors.affiliation: Department of Ophthalmology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.|Department of Stem Cell and Applied Medicine, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Ryuhei
authors.initials: R
authors.lastname: Hayashi
authors.affiliation: Department of Ophthalmology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kobayashi
authors.affiliation: Department of Organ Fabrication, Keio University, School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
authors.forename: Eiji
authors.initials: E
authors.lastname: Kobayashi
authors.affiliation: Department of Ophthalmology, Osaka University Graduate School of Medicine, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Nishida
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000818:Animals; D001393:Azoles / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects; D003315:Cornea / Q000166:cytology / Q000378:metabolism; D003451:Cryoprotective Agents / Q000494:pharmacology*; D006801:Humans; D007035:Hypothermia / Q000378:metabolism; D054833:Isoindoles; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D016566:Organoselenium Compounds / Q000494:pharmacology*; D051381:Rats; D055647:Rats, Transgenic; D017382:Reactive Oxygen Species / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*; D023822:Tissue Engineering; D014021:Tissue Preservation / Q000379:methods
chemical_list: D001393:Azoles; D003451:Cryoprotective Agents; D054833:Isoindoles; D051267:NF-E2-Related Factor 2; C495637:Nfe2l2 protein, rat; D016566:Organoselenium Compounds; D017382:Reactive Oxygen Species; C042986:ebselen; D000255:Adenosine Triphosphate