title: Chronic Low-Grade Inflammation in Childhood Obesity Is Associated with Decreased IL-10 Expression by Monocyte Subsets.
abstract: Chronic low-grade inflammation is related to the development of comorbidities and poor prognosis in obesity. Monocytes are main sources of cytokines and play a pivotal role in inflammation. We evaluated monocyte frequency, phenotype and cytokine profile of monocyte subsets, to determine their association with the pathogenesis of childhood obesity. Children with obesity were evaluated for biochemical and anthropometric parameters. Monocyte subsets were characterized by flow cytometry, considering cytokine production and activation/recognition molecules. Correlation analysis between clinical parameters and immunological data delineated the monocytes contribution for low-grade inflammation. We observed a higher frequency of non-classical monocytes in the childhood obesity group (CO) than normal-weight group (NW). All subsets displayed higher TLR4 expression in CO, but their recognition and antigen presentation functions seem to be diminished due to lower expression of CD40, CD80/86 and HLA-DR. All subsets showed a lower expression of IL-10 in CO and correlation analyses showed changes in IL-10 expression profile. The lower expression of IL-10 may be decisive for the maintenance of the low-grade inflammation status in CO, especially for alterations in non-classical monocytes profile. These cells may contribute to supporting inflammation and loss of regulation in the immune response of children with obesity.
authors.affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Rafael T
authors.initials: RT
authors.lastname: Mattos
authors.affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.|Laboratório de Imunologia Celular e Molecular, Centro de Pesquisa René Rachou, FIOCRUZ, Belo Horizonte, MG, Brasil.
authors.forename: Nayara I
authors.initials: NI
authors.lastname: Medeiros
authors.affiliation: Departamento de Genética, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.|Serviço de Medicina Preventiva da Unimed, Aracaju, SE, Brasil.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Menezes
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Centro de Pesquisa René Rachou, FIOCRUZ, Belo Horizonte, MG, Brasil.
authors.forename: Rafaelle C G
authors.initials: RC
authors.lastname: Fares
authors.affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Eliza P
authors.initials: EP
authors.lastname: Franco
authors.affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.|Instituto Nacional de Ciência e Tecnologia em Doenças Topicais-INCT-DT.
authors.forename: Walderez O
authors.initials: WO
authors.lastname: Dutra
authors.affiliation: Faculdade de Medicina, Departamento de Ciências Básicas da Saúde, Universidade Federal de Mato Grasso, Cuiabá, MT, Brasil.
authors.forename: Fabrício
authors.initials: F
authors.lastname: Rios-Santos
authors.affiliation: Laboratório de Imunologia Celular e Molecular, Centro de Pesquisa René Rachou, FIOCRUZ, Belo Horizonte, MG, Brasil.|Instituto Nacional de Ciência e Tecnologia em Doenças Topicais-INCT-DT.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Correa-Oliveira
authors.affiliation: Laboratório de Biologia das Interações Celulares, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.
authors.forename: Juliana A S
authors.initials: JA
authors.lastname: Gomes
mesh_terms: D018122:B7-1 Antigen / Q000378:metabolism; D051940:B7-2 Antigen / Q000378:metabolism; D019013:CD40 Antigens / Q000378:metabolism; D002648:Child; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008297:Male; D009000:Monocytes / Q000378:metabolism*; D063766:Pediatric Obesity / Q000276:immunology* / Q000378:metabolism*; D017452:Receptors, IgG / Q000378:metabolism
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D019013:CD40 Antigens; C508609:IL10 protein, human; D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D016753:Interleukin-10