title: A microchip electrophoresis-based fluorescence signal amplification strategy for highly sensitive detection of biomolecules.
abstract: We have developed a microchip electrophoresis (MCE)-based fluorescence signal amplification strategy as a universal MCE method for the detection of trace biomolecules. This strategy exhibits high sensitivity and specificity for target molecules, and has been applied for the detection of interferon-gamma (IFN-γ) in human plasma with satisfactory results.
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com jzhao12@163.com.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Qin
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com jzhao12@163.com.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com jzhao12@163.com.
authors.forename: Shuting
authors.initials: S
authors.lastname: Li
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com jzhao12@163.com.
authors.forename: Jingjin
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com jzhao12@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin 541004, China. zhaoshulin001@163.com jzhao12@163.com.
authors.forename: Shulin
authors.identifier: 0000-0002-2560-042X
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Chemistry and Biochemistry, Jackson State University, 1400 Lynch St., Jackson, MS 39217, USA.
authors.forename: Yi-Ming
authors.initials: YM
authors.lastname: Liu
mesh_terms: D046209:Electrophoresis, Microchip / Q000379:methods*; D006801:Humans; D007371:Interferon-gamma / Q000097:blood*; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence
chemical_list: D007371:Interferon-gamma