title: Clinical significance of cell population data (CPD) on Sysmex XN-9000 in septic patients with our without liver impairment.
abstract: BACKGROUND
  This study evaluated the clinical significance of cell population data (CPD) parameters obtained on Sysmex XN-9000 in septic patients admitted to intensive care unit (ICU) and stratified according to liver function.
  
  
  METHODS
  The study population consisted in 84 patients, 44 of whom did not develop sepsis (NS), whereas the remaining 40 developed sepsis (SE) (n=24) or septic shock (SS) (n=16). Two hundred ostensibly healthy blood donors [healthy subjects (HS)], undergoing routine blood testing before a regular blood donation, were studied.
  
  
  RESULTS
  Except for neutrophils and lymphocytes cell size (NE-FCS and LY-Z), all other CPD values were significantly different in ICU patients compared to HS. Neutrophils and monocytes fluorescence intensity (NE-SFL and MO-X) values were significantly higher in SS compared to sepsis and not develop sepsis patients. The value of many parameters was also different according to liver function. Overall, MO-X and neutrophils fluorescence intensity (NE-SFL) exhibited the best performance for diagnosing sepsis in all patients (AUC, 0.75 and 0.72), as well as in those with (AUC, 0.95 and 0.89) or without (AUC, 0.72 for both) liver impairment. These parameters were also significantly correlated with Sequential Organ Failure Assessment (SOFA) score.
  
  
  CONCLUSIONS
  This study suggested that some novel CPD parameters (namely NE-SFL and MO-X) may provide useful information for diagnosis and management of sepsis.
authors.affiliation: Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Buoro
authors.affiliation: Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Michela
authors.initials: M
authors.lastname: Seghezzi
authors.affiliation: Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Vavassori
authors.affiliation: Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Dominoni
authors.affiliation: Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Apassiti Esposito
authors.affiliation: Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Barbara
authors.initials: B
authors.lastname: Manenti
authors.affiliation: Clinical Chemistry Laboratory, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Mecca
authors.affiliation: Intensive Care Unit, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Gianmariano
authors.initials: G
authors.lastname: Marchesi
authors.affiliation: Department of Urology, Papa Giovanni XXIII Hospital, Bergamo, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Castellucci
authors.affiliation: Synlab Laboratory, s.p.a., Monza, Italy.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Azzarà
authors.affiliation: Synlab Laboratory, s.p.a., Monza, Italy.
authors.forename: Cosimo
authors.initials: C
authors.lastname: Ottomano
authors.affiliation: Department of Clinical Biochemistry, University of Verona, Verona, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Lippi
keywords: Automated hematology analyzer; Sysmex; cell population data (CPD); liver impairment; sepsis