title: Involvement of an RNA binding protein containing Alba domain in the stage-specific regulation of beta-amastin expression in Trypanosoma cruzi.
abstract: Amastins are surface glycoproteins, first identified in amastigotes of T. cruzi but later found to be expressed in several Leishmania species, as well as in T. cruzi epimastigotes. Amastins are encoded by a diverse gene family that can be grouped into four subfamilies named α, β, γ, and δ amastins. Differential expression of amastin genes results from regulatory mechanisms involving changes in mRNA stability and/or translational control. Although distinct regulatory elements were identified in the 3' UTR of T. cruzi and Leishmania amastin mRNAs, RNA binding proteins involved with amastin gene regulation have only being characterized in L. infantum where an Alba-domain protein (LiAlba20) able to bind to the 3' UTR of a δ-amastin mRNA was identified. Here we investigated the role of TcAlba30, the LiAlba20 homologue in T. cruzi, in the post transcriptional regulation of amastin genes. TcAlba30 transcripts are present in all stages of the T. cruzi life cycle. RNA immunoprecipitation assays using a transfected cell line expressing a cMyc tagged TcAlba30 revealed that TcAlba30 can interact with β-amastin mRNA. In addition, over-expression of TcAlba30 in epimastigotes resulted in 50% decreased levels of β-amastin mRNAs compared to wild type parasites. Since luciferase assays indicated the presence of regulatory elements in the 3' UTR of β-amastin mRNA and reduced levels of luciferase mRNA were found in parasites over expressing TcAlba30, we conclude that TcAlba30 acts as a T. cruzi RNA binding protein involved in the negative control of β-amastin expression through interactions with its 3'UTR.
authors.affiliation: Laboratorio de Interacciones Moleculares, Facultad de Ciencias, Universidad de la Republica, Montevideo, Uruguay; Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: lperez@fcien.edu.uy.
authors.forename: Leticia
authors.initials: L
authors.lastname: Pérez-Díaz
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil.
authors.forename: Tais Caroline
authors.initials: TC
authors.lastname: Silva
authors.affiliation: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: santuzat@ufmg.br.
authors.forename: Santuza M R
authors.initials: SM
authors.lastname: Teixeira
mesh_terms: D020413:3' Untranslated Regions; D000595:Amino Acid Sequence; D014355:Chagas Disease / Q000469:parasitology; D000066630:Ectopic Gene Expression; D005786:Gene Expression Regulation; D008018:Life Cycle Stages; D008562:Membrane Glycoproteins / Q000235:genetics*; D010802:Phylogeny; D011485:Protein Binding; D000072417:Protein Domains / Q000235:genetics; D011499:Protein Processing, Post-Translational; D015800:Protozoan Proteins / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D014349:Trypanosoma cruzi / Q000145:classification / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D008562:Membrane Glycoproteins; D015800:Protozoan Proteins; D012333:RNA, Messenger; D016601:RNA-Binding Proteins; C088681:amastin protein, Trypanosoma cruzi
keywords: Alba-domain protein; Amastin; Post transcriptional regulation; Trypanosoma cruzi; mRNA