title: Evaluation of in vitro antioxidant and anticancer activity of Allophylus cobbe leaf extracts on DU-145 and PC-3 human prostate cancer cell lines.
abstract: Allophylus cobbe (L.) Raeusch. belonging to the family Sapindaceae, is a commonly distributed small shrub in Western Ghats of India previously reported for its traditional medicinal properties. It is used for the treatment of various ailments. The present study is aimed at investigating preliminary phytochemicals, inducing the determination of the total phenolic contents, antioxidant assays and anticancer activity of A. cobbe leaf extracts on (DU-145) and (PC-3) cell lines. Preliminary phytochemical screening showed a broad spectrum of secondary metabolites. Highest amount of phenolic content was present in aqueous extract (91.96 ± 0.61 mg/g GAE) and it also proved to have the most potent antioxidant activity at a concentration of 100 mg/ml (64.71 ± 0.15%). IC50value was (431.10 ± 15.05 µg/mL) for DU-145 and (362.08 ± 24.17 µg/mL) for PC-3 cell lines while the standard drug paclitaxel showed an IC50value of 0.3 µM/mL. Morphological changes was observed in cancerous cells undergoing apoptosis in human prostate cancer cell lines (DU-145 and PC-3) while the extract showed no cytotoxicity towards normal cells (MEF-L929). It can be concluded that the tested extracts holds significant antioxidant and anticancer activities. However further investigation on lead compounds of A. cobbe will enable its therapeutic use.
authors.affiliation: Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.
authors.forename: Shridhar C
authors.initials: SC
authors.lastname: Ghagane
authors.affiliation: Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India.|Department of Biotechnology, K.L.E'S R. L. Science Institute, (Autonomous), Belagavi, Karnataka, India.
authors.forename: Shridevi I
authors.initials: SI
authors.lastname: Puranik
authors.affiliation: Department of Urology, KLES Kidney Foundation, KLES Dr. Prabhakar Kore Hospital and Medical Research Centre, Belagavi, Karnataka, India.
authors.forename: Rajendra B
authors.initials: RB
authors.lastname: Nerli
authors.affiliation: Department of Biotechnology and Microbiology, Karnatak University, Dharwad, Karnataka, India. murigendra@kud.ac.in.
authors.forename: Murigendra B
authors.initials: MB
authors.lastname: Hiremath
keywords: Allophylus cobbe; Anticancer; Antioxidant; Phytochemicals; Prostate cancer