title: Effects of EMD liquid (Osteogain) on periodontal healing in class III furcation defects in monkeys.
abstract: To evaluate the effect of a novel liquid carrier system of enamel matrix derivative (Osteogain) soaked on an absorbable collagen sponge (ACS) upon periodontal wound healing/regeneration in furcation defects in monkeys.
  
  
  
  The stability of the conventional enamel matrix derivative (Emdogain) and Osteogain adsorbed onto ACS was evaluated by ELISA. Chronic class III furcation defects were created at teeth 36, 37, 46, 47 in three monkeys (Macaca fascicularis). The 12 defects were assigned to one of the following treatments: (1) open flap debridement (OFD) + ACS, (2) OFD+Emdogain/ACS, (3) OFD+Osteogain/ACS, and (4) OFD alone. At 16 weeks following reconstructive surgery, the animals were killed for histological evaluation.
  
  
  
  A 20-60% significantly higher amount of total adsorbed amelogenin was found for ACS-loaded Osteogain when compared to Emdogain. The histomorphometric analysis revealed that both approaches (OFD + Emdogain/ACS and OFD + Osteogain/ACS) resulted in higher amounts of connective tissue attachment and bone formation compared to treatment with OFD + ACS and OFD alone. Furthermore, OFD + Osteogain/ACS group showed higher new attachment formation, cementum, and new bone area.
  
  
  
  Within their limits, the present data indicate that Osteogain possesses favourable physicochemical properties facilitating adsorption of amelogenin on ACS and may additionally enhance periodontal wound healing/regeneration when compared to Emdogain.
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Yoshinori
authors.initials: Y
authors.lastname: Shirakata
authors.affiliation: Department of Periodontology, Nova Southeastern University, Fort Lauderdale, FL, USA.|Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arber, MI, USA.
authors.forename: Richard J
authors.initials: RJ
authors.lastname: Miron
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Sena
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Yukiya
authors.initials: Y
authors.lastname: Shinohara
authors.affiliation: Shin Nippon Biomedical Laboratories, Ltd, Kagoshima, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Horai
authors.affiliation: Robert K. Schenk Laboratory of Oral Histology, University of Bern, Bern, Switzerland.
authors.forename: Dieter D
authors.initials: DD
authors.lastname: Bosshardt
authors.affiliation: Department of Periodontology, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
authors.forename: Kazuyuki
authors.initials: K
authors.lastname: Noguchi
authors.affiliation: Department of Periodontology, School of Dental Medicine, University of Bern, Bern, Switzerland.
authors.forename: Anton
authors.initials: A
authors.lastname: Sculean
mesh_terms: D000818:Animals; D003094:Collagen; D003746:Dental Enamel Proteins / Q000627:therapeutic use*; D017823:Furcation Defects / Q000145:classification / Q000188:drug therapy*; D008252:Macaca fascicularis; D008297:Male; D012074:Remission Induction
chemical_list: D003746:Dental Enamel Proteins; C000621568:Osteogain; C109069:enamel matrix proteins; D003094:Collagen
keywords: absorbable collagen sponge; carrier; class III furcation defect; enamel matrix proteins; periodontal regeneration