title: A rapid method for sensitive profiling of folates from plant leaf by ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometer.
abstract: Previous published methods for the analysis of folates are time consuming because of lengthy sample extraction, clean-up and total running time. This study details the development and validation of a rapid, sensitive and robust method that combines a simple extraction step with ultra-performance liquid chromatography coupled to tandem quadrupole mass spectrometry. Here, we reported application of a tandem quadrupole mass spectrometer to analyze maximum seven vitamers of folate from plant origin. The analytical performance was evaluated by linearity, sensitivity, precision, recovery test and analysis of certified reference materials. The limit of detection and limit of quantification ranged between 0.003 and 0.021μg/100g FW and between 0.011 and 0.041μg/100g FW, respectively; the recovery and precession ranged from 71.27 to 99. 01% and from 1.7 to 7.8% RSD, respectively, depending upon folate vitamers. This newly developed and validated method is rapid (a chromatographic run time of 5min), easy to be performed (no laborious and time consuming clean-up) and can be used to simultaneously analyze seven vitamers of folate from plant sources.
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; Department of Agriculture, Bangabandhu Sheikh Mujibur Rahman Science and Technology University, Gopalganj, 8100, Bangladesh.
authors.forename: M J I
authors.initials: MJI
authors.lastname: Shohag
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Qianying
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; College of Agriculture, Guangxi University, Nanning 530000, People's Republic of China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Farhana Zerin
authors.initials: FZ
authors.lastname: Khan
authors.affiliation: Chair of Analytical Food Chemistry, Technische Universität München, Alte Akademie 10, D-85350 Freising, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Rychlik
authors.affiliation: Indian River Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Fort Pierce, FL 34945, United States.
authors.forename: Zhenli
authors.initials: Z
authors.lastname: He
authors.affiliation: Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resources Science, Zhejiang University, Hangzhou, 310058, People's Republic of China. Electronic address: islam@zju.edu.cn.
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Yang
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000191:economics / Q000379:methods*; D005492:Folic Acid / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D018515:Plant Leaves / Q000737:chemistry*; D010944:Plants / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000191:economics / Q000379:methods*; D013997:Time Factors
chemical_list: D005492:Folic Acid
keywords: Analysis; Extraction; Folate; Leaf sample; Tandem quadrupole mass spectrometer; Ultra-performance liquid chromatography