title: A method comparison study between two hemoglobinometer models (Hemocue Hb 301 and Hb 201+) to measure hemoglobin concentrations and estimate anemia prevalence among women in Preah Vihear, Cambodia.
abstract: BACKGROUND
  Hemoglobin (Hb) concentration is often measured in global health and nutrition surveys to determine anemia prevalence using a portable hemoglobinometer such as the Hemocue® Hb 201+. More recently, a newer model was released (Hemocue Hb 301) utilizing slightly different methods to measure Hb as compared to the older model. The objective was to measure bias and concordance between Hb concentrations using the Hemocue Hb 301 and Hb 201+ models in a rural field setting.
  
  
  METHODS
  Hemoglobin (Hb) concentration was measured using one finger prick of blood (approximately 10 μL) from 175 Cambodian women (18-49 years) using three Hemocue Hb 201+ and three Hb 301 machines. Bias and concordance were measured and plotted.
  
  
  RESULTS
  Overall, mean ± SD Hb concentration was 116 ± 13 g/L using the Hb 201+ and 118 ± 12 g/L using the Hb 301; and anemia prevalence (Hb < 120 g/L) was 58% (n = 102) and 58% (n = 101), respectively. Overall bias ± SD was 2.0 ± 10.5 g/L and concordance (95% CI) was 0.63 (0.54, 0.72). Despite the 2 g/L bias detected between models, anemia prevalence was very similar in both models.
  
  
  CONCLUSIONS
  The two models measured anemia prevalence comparably in this population of women in rural Cambodia.
authors.affiliation: Food, Nutrition and Health, University of British Columbia, Vancouver, BC, Canada.
authors.forename: A I
authors.identifier: http://orcid.org/0000-0002-2527-7909
authors.initials: AI
authors.lastname: Rappaport
authors.affiliation: Food, Nutrition and Health, University of British Columbia, Vancouver, BC, Canada.
authors.forename: C D
authors.initials: CD
authors.lastname: Karakochuk
authors.affiliation: Food, Nutrition and Health, University of British Columbia, Vancouver, BC, Canada.
authors.forename: K C
authors.initials: KC
authors.lastname: Whitfield
authors.affiliation: NutriFood Cambodia, Phnom Penh, Cambodia.
authors.forename: K M
authors.initials: KM
authors.lastname: Kheang
authors.affiliation: Food, Nutrition and Health, University of British Columbia, Vancouver, BC, Canada.|South Australian Health and Medical Research Institute, and the Women's and Children's Health Research Institute, Adelaide, SA, Australia.
authors.forename: T J
authors.initials: TJ
authors.lastname: Green
mesh_terms: D000740:Anemia / Q000097:blood* / Q000453:epidemiology; D002160:Cambodia / Q000453:epidemiology; D005260:Female; D006452:Hemoglobinometry / Q000295:instrumentation; D006454:Hemoglobins / Q000378:metabolism*; D006801:Humans; D015995:Prevalence
chemical_list: D006454:Hemoglobins
keywords: Anemia; Cambodia; Hemocue®; hemoglobin; method comparison; point-of-care testing