title: Ameloblastoma RNA profiling uncovers a distinct non-coding RNA signature.
abstract: Ameloblastoma of the jaws remains the top difficult to treat odontogenic tumour and has a high recurrence rate. New evidence suggests that non-coding RNAs (ncRNAs) play a critical role in tumourgenesis and prognosis of cancer. However, ameloblastoma ncRNA expression data is lacking. Here we present the first report of ameloblastoma ncRNA signatures. A total of 95 ameloblastoma cases and a global array transcriptome technology covering > 285.000 full-length transcripts were used in this two-step analysis. The analysis first identified in a test cohort 31 upregulated ameloblastoma-associated ncRNAs accompanied by signalling pathways of cancer, spliceosome, mRNA surveillance and Wnt. Further validation in an independent cohort points out the long non-coding (lncRNAs) and small nucleolar RNA (snoRNAs): LINC340, SNORD116-25, SNORA11, SNORA21, SNORA47 and SNORA65 as a distinct ncRNA signature of ameloblastoma. Importantly, the presence of these ncRNAs was independent of BRAF-V600E and SMO-L412F mutations, histology type or tumour location, but was positively correlated with the tumour size. Taken together, this study shows a systematic investigation of ncRNA expression of ameloblastoma, and illuminates new diagnostic and therapeutic targets for this invasive odontogenic tumour.
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Haleh
authors.initials: H
authors.lastname: Davanian
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.|Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Anangi
authors.initials: A
authors.lastname: Balasiddaiah
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.|The Clinic of Oral and Maxillofacial Surgery, Karolinska University Hospital, Huddinge, Sweden.
authors.forename: Robert
authors.initials: R
authors.lastname: Heymann
authors.affiliation: Rudbeck Laboratory, Molecular Pathology Unit, Department of Pathology, Uppsala University Hospital and Department of Immunology, Genetics and Pathology, Uppsala University, Sweden.
authors.forename: Magnus
authors.initials: M
authors.lastname: Sundström
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Poppy
authors.initials: P
authors.lastname: Redenström
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Mikael
authors.initials: M
authors.lastname: Silfverberg
authors.affiliation: Department of Biosciences and Nutrition, Bioinformatics and Expression Analysis SciLifeLab, Karolinska Institutet, Huddinge, Sweden.
authors.forename: David
authors.initials: D
authors.lastname: Brodin
authors.affiliation: Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Matti
authors.initials: M
authors.lastname: Sällberg
authors.affiliation: Department of Oncology and Pathology, Karolinska Institutet, Huddinge, Sweden.|Clinical Pathology and Cytology, Karolinska University Hospital, Karolinska University Hospital, Solna, Sweden.
authors.forename: Sven
authors.initials: S
authors.lastname: Lindskog
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.|The Clinic of Oral and Maxillofacial Surgery, Karolinska University Hospital, Huddinge, Sweden.
authors.forename: Carina
authors.initials: C
authors.lastname: Kruger Weiner
authors.affiliation: Department of Dental Medicine, Karolinska Institutet, Huddinge, Sweden.|Department of Laboratory Medicine, Karolinska Institutet, Huddinge, Sweden.
authors.forename: Margaret
authors.initials: M
authors.lastname: Chen
mesh_terms: D000328:Adult; D000368:Aged; D000564:Ameloblastoma / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D015972:Gene Expression Regulation, Neoplastic; D053263:Gene Regulatory Networks; D006801:Humans; D007573:Jaw Neoplasms / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D022661:RNA, Untranslated / Q000235:genetics*; D015398:Signal Transduction
chemical_list: D022661:RNA, Untranslated
keywords: ameloblastoma; biomarkers; gene expression analysis; ncRNA; transcriptome