title: [Relationship between ID1 and EGFR-TKI Resistance  in Non-small Cell Lung Cancer].
abstract: BACKGROUND
  Non-small cell lung cancer (NSCLC) presents the highest morbidity and mortality among malignant tumors worldwide. The overall effective rate of epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) is 30% to 40%, and PFS (progression-free sruvival) is 12 months. However, EGFR-TKI resistance is typical in clinical observations, and this phenomenon significantly affects tumor suppression. To overcome this resistance, a new prognostic factor associated with lung cancer drug resistance should be discovered. This study investigated the relationship between the inhibitor of differentiation 1 (ID1) and non-small cell lung cancer EGFR-TKI resistance in vivo and in vitro to determine any statistical significance and discuss the underlying mechanism.
  
  
  METHODS
  Western blot and qRT-PCR were used to quantify the expression of ID1 in lung cancer. IHC was used to detect the expression of ID1 in pathological tissues (lung cancer tissues and adjacent tissues). MTT was used to detect cell proliferation, in which the cells were treated with gefitinib after being transfected by ID1 slow virus vector. Lung cancer cells were inoculated in nude mice until the tumor diameter grew to certain measurement. Gefitinib treatment was started, and the tumor volume was estimated.
  
  
  RESULTS
  ID1 was highly expressed in NSCLC (P<0.05). Both ID1 expression and drug resistance of EGFR-TKI in NSCLC were positively correlated (P<0.05). The treatment group with gefitinib showed obviously less expression than the control group.
  
  
  CONCLUSIONS
  ID1 is highly expressed in NSCLC. ID1 expression was positively related to drug resistance of EGFR-TKI in NSCLC. Gefitinib can be used to effectively treat NSCLC, and the mechanism may be associated with an increased level of STAT3 phosphorylation.
authors.affiliation: Department of Oncology, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Department of Oncology, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Yinmin
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Oncology, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Chen
authors.affiliation: Department of Oncology, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Oncology, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Qinfang
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Department of Thoracic Surgery, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: Department of Thoracic Surgery, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Boxiong
authors.initials: B
authors.lastname: Xie
authors.affiliation: Department of Oncology, Pulmonary Disease Hospital of Tongji University, Shanghai 200433, China.
authors.forename: Songwen
authors.initials: S
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002289:Carcinoma, Non-Small-Cell Lung / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D019008:Drug Resistance, Neoplasm; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism; D006801:Humans; D051798:Inhibitor of Differentiation Protein 1 / Q000235:genetics* / Q000378:metabolism; D008175:Lung Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D047428:Protein Kinase Inhibitors / Q000008:administration & dosage*
chemical_list: C497453:ID1 protein, human; C497455:Idb1 protein, mouse; D051798:Inhibitor of Differentiation Protein 1; D047428:Protein Kinase Inhibitors; D066246:ErbB Receptors