title: Magnetite Nanoparticles for Stem Cell Labeling with High Efficiency and Long-Term in Vivo Tracking.
abstract: Superparamagnetic iron oxide nanoparticles (SPIO-PAA), ultrasmall iron oxide nanoparticles (USPIO-PAA), and glucosamine-modified iron oxide nanoparticles (USPIO-PAA-GlcN) were studied as mesenchymal stem cell (MSCs) labels for cell tracking applications by magnetic resonance imaging (MRI). Pronounced differences were found in the labeling performance of the three samples in terms of cellular dose and labeling efficiency. In combination with polylysine, SPIO-PAA showed nonhomogeneous cell internalization, while for USPIO-PAA no uptake was found. On the contrary, USPIO-PAA-GlcN featured high cellular uptake and biocompatibility, and sensitive detection in both in vitro and in vivo experiments was found by MRI, showing that glucosamine functionalization can be an efficient strategy to increase cell uptake of ultrasmall iron oxide nanoparticles by MSCs.
authors.affiliation: International Iberian Nanotechnology Laboratory , Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
authors.forename: Noelia
authors.identifier: 0000-0001-6821-4222
authors.initials: N
authors.lastname: Guldris
authors.forename: Bárbara
authors.initials: B
authors.lastname: Argibay
authors.affiliation: International Iberian Nanotechnology Laboratory , Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
authors.forename: Juan
authors.identifier: 0000-0002-2028-3234
authors.initials: J
authors.lastname: Gallo
authors.forename: Ramón
authors.initials: R
authors.lastname: Iglesias-Rey
authors.affiliation: International Iberian Nanotechnology Laboratory , Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
authors.forename: Enrique
authors.initials: E
authors.lastname: Carbó-Argibay
authors.affiliation: International Iberian Nanotechnology Laboratory , Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
authors.forename: Yury V
authors.identifier: 0000-0001-7493-1762
authors.initials: YV
authors.lastname: Kolen'ko
authors.forename: Francisco
authors.initials: F
authors.lastname: Campos
authors.forename: Tomás
authors.initials: T
authors.lastname: Sobrino
authors.affiliation: International Iberian Nanotechnology Laboratory , Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
authors.forename: Laura M
authors.identifier: 0000-0001-8194-6014
authors.initials: LM
authors.lastname: Salonen
authors.affiliation: International Iberian Nanotechnology Laboratory , Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
authors.forename: Manuel
authors.initials: M
authors.lastname: Bañobre-López
authors.forename: José
authors.initials: J
authors.lastname: Castillo
authors.affiliation: International Iberian Nanotechnology Laboratory , Av. Mestre José Veiga s/n, 4715-330 Braga, Portugal.
authors.forename: José
authors.identifier: 0000-0002-5059-3196
authors.initials: J
authors.lastname: Rivas
mesh_terms: D000818:Animals; D001692:Biological Transport; D002470:Cell Survival / Q000187:drug effects; D058948:Cell Tracking / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008297:Male; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D010316:Particle Size; D051381:Rats; D013194:Staining and Labeling
chemical_list: D058185:Magnetite Nanoparticles