title: Lipopeptides as therapeutics: applications and in vivo quantitative analysis.
abstract: A number of novel lipopeptides have been studied for their possible therapeutic potential. These studies should be supported by the appropriate analytical tools not only for novel potential drugs but also for their metabolites, precursors and side products. Lipopeptides have specific physicochemical properties that make them successful in medical applications. However, there are some difficulties with their qualitative and quantitative analyses in biological samples. Therefore, reliable, sensitive and robust analytical methods are in high demand. The main interest of our review is to describe a selection of specific and important properties of lipopeptides, and the analytical methods currently utilized for their characterization and determination in biological samples. A comparison of the pros and cons of immunomethods versus LC-MS methods is discussed in detail.
authors.affiliation: University of Chemistry & Technology Prague, Department of Analytical Chemistry, Technická 5, 16628 Prague, Czech Republic.|Institute of Organic Chemistry & Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610 Prague 6, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Zemenová
authors.affiliation: University of Chemistry & Technology Prague, Department of Analytical Chemistry, Technická 5, 16628 Prague, Czech Republic.
authors.forename: David
authors.initials: D
authors.lastname: Sýkora
authors.affiliation: Institute of Organic Chemistry & Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610 Prague 6, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Maletínská
authors.affiliation: Institute of Organic Chemistry & Biochemistry, Academy of Sciences of the Czech Republic, Flemingovo nám. 2, 16610 Prague 6, Czech Republic.|Institute of Physiology, Academy of Sciences of the Czech Republic, Videnska 1083, 14220 Prague, Czech Republic.
authors.forename: Jaroslav
authors.initials: J
authors.lastname: Kuneš
mesh_terms: D002851:Chromatography, High Pressure Liquid; D054439:Ghrelin / Q000032:analysis / Q000302:isolation & purification; D006801:Humans; D007118:Immunoassay; D055666:Lipopeptides / Q000032:analysis* / Q000302:isolation & purification; D013058:Mass Spectrometry; D011485:Protein Binding; D052616:Solid Phase Extraction
chemical_list: D054439:Ghrelin; D055666:Lipopeptides
keywords: LC–MS; immunomethods; lipopeptides