title: Chitosan nanoparticles for combined drug delivery and magnetic hyperthermia: From preparation to in vitro studies.
abstract: Chitosan nanoparticles (CSNPs) ionically crosslinked with tripolyphosphate salts (TPP) were employed as nanocarriers in combined drug delivery and magnetic hyperthermia (MH) therapy. To that aim, three different ferrofluid concentrations and a constant 5-fluorouracil (5-FU) concentration were efficiently encapsulated to yield magnetic CSNPs with core-shell morphology. In vitro experiments using normal cells, fibroblasts (FHB) and cancer cells, human glioblastoma A-172, showed that CSNPs presented a dose-dependent cytotoxicity and that they were successfully uptaken into both cell lines. The application of a MH treatment in A-172 cells resulted in a cell viability of 67-75% whereas no significant reduction of cell viability was observed for FHB. However, the A-172 cells showed re-growth populations 4h after the application of the MH treatment when CSNPs were loaded only with ferrofluid. Finally, a combined effect of MH and 5-FU release was observed with the application of a second MH treatment for CSNPs exhibiting a lower amount of released 5-FU. This result demonstrates the potential of CSNPs for the improvement of MH therapies.
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (CSIC), c/Juan de la Cierva, 3, 28006 Madrid, Spain.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Zamora-Mora
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (CSIC), c/Juan de la Cierva, 3, 28006 Madrid, Spain; CIBER-BBN, c/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Fernández-Gutiérrez
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (CSIC), c/Juan de la Cierva, 3, 28006 Madrid, Spain; CIBER-BBN, c/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain.
authors.forename: Álvaro
authors.initials: Á
authors.lastname: González-Gómez
authors.affiliation: Nanoscience Institute of Aragón, University of Zaragoza, Mariano Esquillor s/n, 50018 Zaragoza, Spain; Department of Condensed Matter Physics, University of Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Sanz
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (CSIC), c/Juan de la Cierva, 3, 28006 Madrid, Spain; CIBER-BBN, c/Monforte de Lemos 3-5, Pabellón 11, 28029 Madrid, Spain.
authors.forename: Julio San
authors.initials: JS
authors.lastname: Román
authors.affiliation: Nanoscience Institute of Aragón, University of Zaragoza, Mariano Esquillor s/n, 50018 Zaragoza, Spain; Department of Condensed Matter Physics, University of Zaragoza, Pedro Cerbuna 12, 50009 Zaragoza, Spain.
authors.forename: Gerardo F
authors.initials: GF
authors.lastname: Goya
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (CSIC), c/Juan de la Cierva, 3, 28006 Madrid, Spain. Electronic address: rhernandez@ictp.csic.es.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Hernández
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (CSIC), c/Juan de la Cierva, 3, 28006 Madrid, Spain.
authors.forename: Carmen
authors.initials: C
authors.lastname: Mijangos
mesh_terms: D045744:Cell Line, Tumor; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems; D005472:Fluorouracil; D006801:Humans; D066298:In Vitro Techniques; D008280:Magnetics; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D048271:Chitosan; D005472:Fluorouracil
keywords: Cancer cells; Chitosan; Cytotoxicity; Drug delivery; In vitro studies; Magnetic hyperthermia; Magnetic nanoparticles; Uptake