title: A Critical Role for Toxoplasma gondii Vacuolar Protein Sorting VPS9 in Secretory Organelle Biogenesis and Host Infection.
abstract: Accurate sorting of proteins to the three types of parasite-specific secretory organelles namely rhoptry, microneme and dense granule in Toxoplasma gondii is crucial for successful host cell invasion by this obligate intracellular parasite. Despite its tiny body architecture and limited trafficking machinery, T. gondii relies heavily on transport of vesicles containing proteins, lipids and important virulence-like factors that are delivered to these secretory organelles. However, our understanding on how trafficking of vesicles operates in the parasite is still limited. Here, we show that the T. gondii vacuolar protein sorting 9 (TgVps9), has guanine nucleotide exchange factor (GEF) activity towards Rab5a and is crucial for sorting of proteins destined to secretory organelles. Our results illuminate features of TgVps9 protein as a key trafficking facilitator that regulates protein maturation, secretory organelle formation and secretion, thereby ensuring a primary role in host infection by T. gondii.
authors.affiliation: Laboratory of Cellular and Molecular Biology of Toxoplasma, Université de Lille, Institut Pasteur de Lille, Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, 59000, Lille, France.
authors.forename: Takaya
authors.initials: T
authors.lastname: Sakura
authors.affiliation: Institute for Advanced Biosciences (IAB), INSERM U1209, CNRS UMR5309, Université Grenoble Alpes, 38700, Grenoble, France.
authors.forename: Fabien
authors.initials: F
authors.lastname: Sindikubwabo
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, Molecular Mechanisms of Intracellular Transport Group, 75248, Paris, France.
authors.forename: Lena K
authors.initials: LK
authors.lastname: Oesterlin
authors.affiliation: Institut Curie, PSL Research University, CNRS UMR144, Molecular Mechanisms of Intracellular Transport Group, 75248, Paris, France.
authors.forename: Hugo
authors.initials: H
authors.lastname: Bousquet
authors.affiliation: Laboratory of Cell Physiology, INSERM U 1003, Université de Lille, 59655, Villeneuve d'Ascq, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Slomianny
authors.affiliation: Institute for Advanced Biosciences (IAB), INSERM U1209, CNRS UMR5309, Université Grenoble Alpes, 38700, Grenoble, France.
authors.forename: Mohamed-Ali
authors.initials: MA
authors.lastname: Hakimi
authors.affiliation: Laboratoire de Biologie Cellulaire Comparative des Apicomplexes, INSERM U1016, CNRS UMR8104, Institut Cochin, 75014, Paris, France.
authors.forename: Gordon
authors.initials: G
authors.lastname: Langsley
authors.affiliation: Laboratory of Cellular and Molecular Biology of Toxoplasma, Université de Lille, Institut Pasteur de Lille, Center for Infection and Immunity of Lille, INSERM U 1019, CNRS UMR 8204, 59000, Lille, France.
authors.forename: Stanislas
authors.initials: S
authors.lastname: Tomavo
mesh_terms: D002460:Cell Line; D006801:Humans; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D055571:Secretory Pathway; D014122:Toxoplasma / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D014123:Toxoplasmosis / Q000235:genetics / Q000378:metabolism*; D022161:Transport Vesicles / Q000235:genetics / Q000378:metabolism*; D033921:Vesicular Transport Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D033921:Vesicular Transport Proteins