title: Separation of phenolic acids from sugarcane rind by online solid-phase extraction with high-speed counter-current chromatography.
abstract: Sugarcane rind contains some functional phenolic acids. The separation of these compounds from sugarcane rind is able to realize the integrated utilization of the crop and reduce environment pollution. In this paper, a novel protocol based on interfacing online solid-phase extraction with high-speed counter-current chromatography (HSCCC) was established, aiming at improving and simplifying the process of phenolic acids separation from sugarcane rind. The conditions of online solid-phase extraction with HSCCC involving solvent system, flow rate of mobile phase as well as saturated extent of absorption of solid-phase extraction were optimized to improve extraction efficiency and reduce separation time. The separation of phenolic acids was performed with a two-phase solvent system composed of butanol/acetic acid/water at a volume ratio of 4:1:5, and the developed online solid-phase extraction with HSCCC method was validated and successfully applied for sugarcane rind, and three phenolic acids including 6.73 mg of gallic acid, 10.85 mg of p-coumaric acid, and 2.78 mg of ferulic acid with purities of 60.2, 95.4, and 84%, respectively, were obtained from 150 mg sugarcane rind crude extracts. In addition, the three different elution methods of phenolic acids purification including HSCCC, elution-extrusion counter-current chromatography and back-extrusion counter-current chromatography were compared.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University Of Chemical Technology, Beijing, P. R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University Of Chemical Technology, Beijing, P. R. China.
authors.forename: Yingtong
authors.initials: Y
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University Of Chemical Technology, Beijing, P. R. China.
authors.forename: Ronglong
authors.initials: R
authors.lastname: Xie
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University Of Chemical Technology, Beijing, P. R. China.
authors.forename: Weilun
authors.initials: W
authors.lastname: Hu
authors.affiliation: China National Institute of Standardization, Beijing, P. R. China.
authors.forename: Xingjun
authors.initials: X
authors.lastname: Xi
authors.affiliation: China National Institute of Standardization, Beijing, P. R. China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Chu
authors.affiliation: China National Institute of Standardization, Beijing, P. R. China.
authors.forename: Genlai
authors.initials: G
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University Of Chemical Technology, Beijing, P. R. China.
authors.forename: Nusrat
authors.initials: N
authors.lastname: Shaheen
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University Of Chemical Technology, Beijing, P. R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wei
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003377:Countercurrent Distribution; D062385:Hydroxybenzoates / Q000302:isolation & purification*; D010936:Plant Extracts / Q000737:chemistry*; D031786:Saccharum / Q000737:chemistry*; D052616:Solid Phase Extraction
chemical_list: D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid
keywords: high-speed counter-current chromatography; phenolic acids; solid-phase extraction; sugarcane rind