title: Assays for the Screening and Characterization of Tau Aggregation Inhibitors.
abstract: Aggregation of tau protein is a pathological hallmark of Alzheimer's disease and other neurodegenerative tauopathies. Inhibition of tau aggregation could provide a method for the treatment of these disorders. Methods to identify tau aggregation inhibitors (TAIs) in vitro are useful and here we describe assays for TAIs using purified recombinant tau protein fragments in a cell-free immunoassay format and a stably transfected cell model to create a more physiological environment.
authors.affiliation: School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, AB25 2ZP, Scotland, UK.|TauRx Therapeutics Ltd., Aberdeen, Scotland, UK.
authors.forename: Janet E
authors.initials: JE
authors.lastname: Rickard
authors.affiliation: School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, AB25 2ZP, Scotland, UK.|TauRx Therapeutics Ltd., Aberdeen, Scotland, UK.
authors.forename: David
authors.initials: D
authors.lastname: Horsley
authors.affiliation: School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, AB25 2ZP, Scotland, UK.|TauRx Therapeutics Ltd., Aberdeen, Scotland, UK.
authors.forename: Claude M
authors.initials: CM
authors.lastname: Wischik
authors.affiliation: School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Aberdeen, AB25 2ZP, Scotland, UK. c.harrington@abdn.ac.uk.|TauRx Therapeutics Ltd., Aberdeen, Scotland, UK. c.harrington@abdn.ac.uk.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Harrington
mesh_terms: D000544:Alzheimer Disease / Q000188:drug therapy / Q000378:metabolism*; D000818:Animals; D001681:Biological Assay / Q000379:methods*; D002460:Cell Line; D004797:Enzyme-Linked Immunosorbent Assay; D007118:Immunoassay; D051379:Mice; D018696:Neuroprotective Agents / Q000627:therapeutic use; D066263:Protein Aggregation, Pathological / Q000188:drug therapy* / Q000378:metabolism*; D016875:tau Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*
chemical_list: D018696:Neuroprotective Agents; D016875:tau Proteins
keywords: Alzheimer’s disease; ELISA; Immunoassay; Tau aggregation inhibitor; Tau protein