title: Synthesis of polypyrrole nanowires with positive effect on MC3T3-E1 cell functions through electrical stimulation.
abstract: Conducting polymer polypyrrole (PPy) possesses good biocompatibility and conductivity and has been used as functional coatings in bone tissue regeneration. In this study, a cholic acid doped PPy nanowires (PPy NWs) coating was electrochemically polymerized on the surface of titanium (Ti). The porous intertwined PPy NWs coating exhibited excellent electrical conductivity and electrochemical activity, better hydrophilicity and higher surface energy. In vitro cell experiments demonstrated that the PPy NWs coating together with a 10μA substrate-mediate electrical stimulation (ES) was capable to positive regulate the functions of MC3T3-E1 such as cell adhesion, proliferation and differentiation. Further long-term functions of cell tests including alkaline phosphatase (ALP) activity, bone-carboxyglutamic acid-containing protein (BGP) and calcium deposition were all thoroughly increased. These confirmed that the combination of PPy NWs and ES could accelerate MC3T3-E1 cells mature and osteogenesis. Hence, the PPy NWs coating was an electro bioactive coating and may have potential applications in the treatment of bone damage repairing and regeneration with ES.
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
authors.forename: Shihui
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China; Chinese Academy of Sciences-Max Planck Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science, Shanghai 200031, China. Electronic address: mujing@picb.ac.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Mu
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
authors.forename: Lingfeng
authors.initials: L
authors.lastname: Dai
authors.affiliation: Department of Stomatology, The Affiliated Zhongshan Hospital of Xiamen University, Xiamen 361005, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Biomaterials, College of Materials, Xiamen University, Xiamen 361005, China. Electronic address: gedt@xmu.edu.cn.
authors.forename: Dongtao
authors.initials: D
authors.lastname: Ge
mesh_terms: D000818:Animals; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D004553:Electric Conductivity; D051379:Mice; D053770:Nanowires / Q000737:chemistry*; D010012:Osteogenesis; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*
chemical_list: D020099:Coated Materials, Biocompatible; D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole
keywords: Electrical stimulation; MC3T3-E1; Nanostructure; Polypyrrole; Titanium