title: A comprehensive profiling of sulfatides in myelin from mouse brain using liquid chromatography coupled to high-resolution accurate tandem mass spectrometry.
abstract: Sulfatides are sulfoglycolipids found in the myelin sheath. The composition ratio of sulfatide molecular species changes with age, and it has also been associated with the pathogenesis of various human central nervous system diseases. However, profiling sulfatides in biological samples is difficult, due to the great variety of molecular species. In this work, a new, easy and reliable liquid chromatography-electrospray tandem mass spectrometry (LC-ESI(+)-MS/MS) method has been developed to profile sulfatide content in biological samples of myelin. The 'wrong-way-round' ionization effect has been described for this type of molecules for the first time, making it possible to correctly identify as many as 37 different sulfatides in mouse brain myelin samples, including molecules with different fatty acid chain lengths and varying degrees of unsaturation and hydroxylation. A chemometric analysis of their relative abundances showed that the main difference among individuals of different ages was the content of sulfatides with odd-numbered fatty acid chains, in addition to hydroxylated species.
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Pintado-Sierra
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain; Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca "La Peraleda", 45071, Toledo, Spain.
authors.forename: I
authors.initials: I
authors.lastname: García-Álvarez
authors.affiliation: Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca "La Peraleda", 45071, Toledo, Spain; Grupo de Neurobiología del Desarrollo-GNDe, Instituto Cajal (CSIC), Doctor Arce 37, 28002, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Bribián
authors.affiliation: Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca "La Peraleda", 45071, Toledo, Spain.
authors.forename: E M
authors.initials: EM
authors.lastname: Medina-Rodríguez
authors.affiliation: Instituto de Química-Física 'Rocasolano' (CSIC), Serrano 119, 28006, Madrid, Spain.
authors.forename: R
authors.initials: R
authors.lastname: Lebrón-Aguilar
authors.affiliation: Instituto de Ciencia y Tecnología de Polímeros (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Garrido
authors.affiliation: Grupo de Neurobiología del Desarrollo-GNDe, Hospital Nacional de Parapléjicos, Finca "La Peraleda", 45071, Toledo, Spain; Grupo de Neurobiología del Desarrollo-GNDe, Instituto Cajal (CSIC), Doctor Arce 37, 28002, Madrid, Spain.
authors.forename: F
authors.initials: F
authors.lastname: de Castro
authors.affiliation: Instituto de Química Orgánica General (CSIC), Juan de la Cierva 3, 28006, Madrid, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Fernández-Mayoralas
authors.affiliation: Instituto de Química-Física 'Rocasolano' (CSIC), Serrano 119, 28006, Madrid, Spain. Electronic address: je.quintanilla@iqfr.csic.es.
authors.forename: J E
authors.initials: JE
authors.lastname: Quintanilla-López
mesh_terms: D000375:Aging; D000818:Animals; D001923:Brain Chemistry; D002853:Chromatography, Liquid; D051379:Mice; D008810:Mice, Inbred C57BL; D009186:Myelin Sheath / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization; D013433:Sulfoglycosphingolipids / Q000032:analysis*; D053719:Tandem Mass Spectrometry
chemical_list: D013433:Sulfoglycosphingolipids
keywords: Accurate mass; Liquid chromatography-tandem mass spectrometry; Mouse brain; Myelin; Sulfatides