title: Tissue specific transcript profiling of wheat phosphate transporter genes and its association with phosphate allocation in grains.
abstract: Approaches enabling efficient phosphorus utilization in crops are of great importance. In cereal crop like wheat, utilization of inorganic phosphate (Pi) is high and mature grains are the major sink for Pi utilization and storage. Research that addresses the importance of the Pi homeostasis in developing grains is limited. In an attempt to understand the Pi homeostasis in developing wheat grains, we identified twelve new phosphate transporters (PHT), these are phyologentically well distributed along with the members reported from Arabidopsis and rice. Enhanced expression of PHT1-subfamily genes was observed in roots subjected to the Pi starvation suggesting their active role in Pi homeostasis. Differential expression patterns of all the PHT genes during grain filling stages suggested their importance in the filial tissues. Additionally, high accumulation of Pi and total P in aleurone correlates well with the expression of TaPHTs and other phosphate starvation related genes. Tissue specific transcript accumulation of TaPHT1.1, TaPHT1.2, TaPHT1.4 in aleurone; TaPHT3.1 in embryo and TaPHT4.2 in the endosperm was observed. Furthermore, their transcript abundance was affected in low phytate wheat grains. Altogether, this study helps in expanding the knowledge and prioritize the candidate wheat Pi-transporters to modulate the Pi homeostasis in cereal grains.
authors.affiliation: National Agri-Food Biotechnology Institute (Department of Biotechnology, Government of India), C-127, Industrial Area, S.A.S. Nagar, Phase 8, Mohali-160071, Punjab, India.
authors.forename: Vishnu
authors.initials: V
authors.lastname: Shukla
authors.affiliation: National Agri-Food Biotechnology Institute (Department of Biotechnology, Government of India), C-127, Industrial Area, S.A.S. Nagar, Phase 8, Mohali-160071, Punjab, India.
authors.forename: Mandeep
authors.initials: M
authors.lastname: Kaur
authors.affiliation: National Agri-Food Biotechnology Institute (Department of Biotechnology, Government of India), C-127, Industrial Area, S.A.S. Nagar, Phase 8, Mohali-160071, Punjab, India.
authors.forename: Sipla
authors.initials: S
authors.lastname: Aggarwal
authors.affiliation: National Agri-Food Biotechnology Institute (Department of Biotechnology, Government of India), C-127, Industrial Area, S.A.S. Nagar, Phase 8, Mohali-160071, Punjab, India.
authors.forename: Kaushal Kumar
authors.initials: KK
authors.lastname: Bhati
authors.affiliation: Department of Biotechnology, University Institute of Engineering and Technology (UIET), Panjab University, Chandigarh, India.
authors.forename: Jaspreet
authors.initials: J
authors.lastname: Kaur
authors.affiliation: National Agri-Food Biotechnology Institute (Department of Biotechnology, Government of India), C-127, Industrial Area, S.A.S. Nagar, Phase 8, Mohali-160071, Punjab, India.
authors.forename: Shrikant
authors.initials: S
authors.lastname: Mantri
authors.affiliation: National Agri-Food Biotechnology Institute (Department of Biotechnology, Government of India), C-127, Industrial Area, S.A.S. Nagar, Phase 8, Mohali-160071, Punjab, India.
authors.forename: Ajay K
authors.initials: AK
authors.lastname: Pandey
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D001483:Base Sequence; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant / Q000235:genetics; D018745:Genome, Plant / Q000235:genetics; D012275:Oryza / Q000235:genetics / Q000378:metabolism*; D028061:Phosphate Transport Proteins / Q000096:biosynthesis / Q000235:genetics*; D010710:Phosphates / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D017422:Sequence Analysis, DNA; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D028061:Phosphate Transport Proteins; D010710:Phosphates; D012333:RNA, Messenger