title: Development of Highly Selective Fluorescent Probe Enabling Flow-Cytometric Isolation of ALDH3A1-Positive Viable Cells.
abstract: Aldehyde dehydrogenase (ALDH) is overexpressed in some subpopulations of stem cells and cancer cells. We have designed and synthesized the first selective fluorescent probe for class 3 ALDH (ALDH3A1). This probe enabled the visualization of ALDH3A1-positive cells by fluorescence microscopy as well as flow-cytometric isolation of ALDH3A1-positive viable cells from a human Caucasian esophageal squamous cell line (OE21) that heterogeneously expresses ALDH3A1.
authors.affiliation: Division of Gene Regulation, Institute for Advanced Medical Research, Graduate School of Medicine, Keio University , 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582 Japan.
authors.forename: Atsushi
authors.identifier: 0000-0001-7057-7647
authors.initials: A
authors.lastname: Yagishita
authors.affiliation: CREST, Japan Science and Technology Agency , 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Ueno
authors.affiliation: Research Institute for Biomedical Sciences, Tokyo University of Science , 2641 Yamazaki, Noda, Chiba 278-8510, Japan.
authors.forename: Hiroyasu
authors.initials: H
authors.lastname: Esumi
authors.affiliation: Division of Gene Regulation, Institute for Advanced Medical Research, Graduate School of Medicine, Keio University , 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582 Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Saya
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Kaneko
authors.forename: Katsuya
authors.initials: K
authors.lastname: Tsuchihara
authors.affiliation: CREST, Japan Science and Technology Agency , 7 Gobancho, Chiyoda-ku, Tokyo 102-0076, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
mesh_terms: D000444:Aldehyde Dehydrogenase / Q000378:metabolism*; D045744:Cell Line, Tumor; D002470:Cell Survival; D015195:Drug Design; D005434:Flow Cytometry; D005456:Fluorescent Dyes / Q000378:metabolism*; D006801:Humans; D008856:Microscopy, Fluorescence
chemical_list: D005456:Fluorescent Dyes; C480021:ALDH3A1 protein, human; D000444:Aldehyde Dehydrogenase