title: The Suppressed Induction of Human Mature Cytotoxic T Lymphocytes Caused by Asbestos Is Not due to Interleukin-2 Insufficiency.
abstract: We previously reported that exposure to chrysotile B (CB) asbestos suppressed the induction of mature cytotoxic T lymphocytes (CTLs) during mixed lymphocyte reaction assays (MLRs) with a decrease in the proliferation of immature CTLs. However, the mechanism responsible for the effect of asbestos fibers on the differentiation of CTLs remains unclear. Since interleukin-2 (IL-2) is a regulator of T lymphocyte proliferation, we examined the effect of IL-2 addition on suppressed CTL differentiation in CB-exposed cultures using flow cytometry (FCM). When IL-2 was added at 1 ng/mL on the second day of MLRs, the asbestos-caused decreases in the proliferation and percentages of CD25+and CD45RO+cells in CD8+lymphocytes were not recovered by IL-2 addition, although the decrease in percentage of granzyme B+cells was partially recovered. CD8+lymphocytes from the IL-2-treated culture with asbestos showed the same degree of cytotoxicity as those in cultures without IL-2 or asbestos. These findings indicate that IL-2 insufficiency is not the main cause for the suppressed induction of CTLs by asbestos exposure, although they suggest a potential for the improvement of such suppressed CTL functions. Secretory factors other than IL-2 in addition to membrane-bound stimulatory molecules may play a role in asbestos-caused suppressed CTL differentiation.
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan.
authors.forename: Naoko
authors.initials: N
authors.lastname: Kumagai-Takei
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan.
authors.forename: Yasumitsu
authors.identifier: 0000-0002-3432-077X
authors.initials: Y
authors.lastname: Nishimura
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan.
authors.forename: Hidenori
authors.identifier: 0000-0001-9362-4565
authors.initials: H
authors.lastname: Matsuzaki
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan.
authors.forename: Suni
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Yoshitome
authors.affiliation: Department of Dermatology, Kawasaki Medical School, Kurashiki 701-0192, Japan.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Hayashi
authors.affiliation: Department of Hygiene, Kawasaki Medical School, Kurashiki 701-0192, Japan.
authors.forename: Takemi
authors.identifier: 0000-0001-6509-2311
authors.initials: T
authors.lastname: Otsuki
mesh_terms: D001194:Asbestos / Q000009:adverse effects*; D015415:Biomarkers; D018414:CD8-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D003602:Cytotoxicity, Immunologic; D053804:Granzymes / Q000378:metabolism; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology / Q000378:metabolism; D007165:Immunosuppression Therapy; D007376:Interleukin-2 / Q000378:metabolism* / Q000494:pharmacology; D007963:Leukocytes, Mononuclear / Q000276:immunology / Q000378:metabolism; D008213:Lymphocyte Activation / Q000187:drug effects / Q000276:immunology; D007959:Lymphocyte Culture Test, Mixed; D013602:T-Lymphocytes, Cytotoxic / Q000166:cytology / Q000276:immunology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D007376:Interleukin-2; D001194:Asbestos; D053804:Granzymes