title: Effect of Lactobacillus plantarum Tennozu-SU2 on Salmonella Typhimurium Infection in Human Enterocyte-Like HT-29-Luc Cells and BALB/c Mice.
abstract: The probiotic properties and inhibitory effect on Salmonella Typhimurium adhesion on human enterocyte-like HT-29-Luc cells of three Lactobacillus plantarum strains isolated from fermented fish, beach sand and a coastal plant were determined. Compared with the type strain L. plantarum NBRC 15891T, which was isolated from pickled cabbage, L. plantarum Tennozu-SU2 isolated from the acorn of a coastal tree showed high autoaggregation in de Man, Rogosa and Sharpe (MRS) broth and an antagonistic effect against S. Typhimurium in brain heart infusion (BHI) broth. Furthermore, heat-killed L. plantarum Tennozu-SU2 cells inhibited S. Typhimurium adhesion on HT-29-Luc cells. Both live and heat-killed L. plantarum Tennozu-SU2 cells showed an inhibitory effect on gut colonisation in BALB/c mice, as assessed by viable Salmonella count in faecal samples and by invasion into liver and spleen tissues. The properties shown in this study suggest that L. plantarum Tennozu-SU2 is useful as a starter and probiotic bacteria in functional food material.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan.
authors.forename: Shino
authors.initials: S
authors.lastname: Hirano
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan.
authors.forename: Yasushi
authors.initials: Y
authors.lastname: Yokota
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan.
authors.forename: Mika
authors.initials: M
authors.lastname: Eda
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan. kuda@kaiyodai.ac.jp.
authors.forename: Takashi
authors.initials: T
authors.lastname: Kuda
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan.
authors.forename: Ayane
authors.initials: A
authors.lastname: Shikano
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology, 4-5-7 Konan, Minato City, Tokyo, 108-8477, Japan.
authors.forename: Bon
authors.initials: B
authors.lastname: Kimura
mesh_terms: D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects; D001937:Brassica / Q000382:microbiology*; D020895:Enterocytes / Q000187:drug effects* / Q000382:microbiology; D005243:Feces / Q000382:microbiology; D019073:HT29 Cells; D006801:Humans; D048191:Lactiplantibacillus plantarum / Q000502:physiology*; D008099:Liver / Q000382:microbiology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D019936:Probiotics / Q000008:administration & dosage*; D012480:Salmonella Infections / Q000188:drug therapy* / Q000382:microbiology; D012486:Salmonella typhimurium / Q000502:physiology*
keywords: BALB/c mice; HT-29-Luc cells; Lactobacillus plantarum; Salmonella Typhimurium