title: Phosphatidylinositol-3-phosphate in the regulation of autophagy membrane dynamics.
abstract: Phosphatidylinositol-3-phosphate (PI3P) is a key player in membrane dynamics and trafficking regulation. Most PI3P is associated with endosomal membranes and with the autophagosome preassembly machinery, presumably at the endoplasmic reticulum. The enzyme responsible for most PI3P synthesis, VPS34 and proteins such as Beclin1 and ATG14L that regulate PI3P levels are positive modulators of autophagy initiation. It had been assumed that a local PI3P pool was present at autophagosomes and preautophagosomal structures, such as the omegasome and the phagophore. This was recently confirmed by the demonstration that PI3P-binding proteins participate in the complex sequence of signalling that results in autophagosome assembly and activity. Here we summarize the historical discoveries of PI3P lipid kinase involvement in autophagy, and we discuss the proposed role of PI3P during autophagy, notably during the autophagosome biogenesis sequence.
authors.affiliation: Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Paris, France.|Université Paris Descartes-Sorbonne Paris Cité, France.
authors.forename: Anna Chiara
authors.initials: AC
authors.lastname: Nascimbeni
authors.affiliation: Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Paris, France.|Université Paris Descartes-Sorbonne Paris Cité, France.
authors.forename: Patrice
authors.initials: P
authors.lastname: Codogno
authors.affiliation: Institut Necker-Enfants Malades (INEM), INSERM U1151-CNRS UMR 8253, Paris, France.|Université Paris Descartes-Sorbonne Paris Cité, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Morel
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000378:metabolism; D000818:Animals; D000071182:Autophagosomes / Q000201:enzymology / Q000502:physiology*; D001343:Autophagy; D000071183:Autophagy-Related Proteins / Q000378:metabolism; D000071186:Beclin-1 / Q000378:metabolism; D058541:Class II Phosphatidylinositol 3-Kinases / Q000378:metabolism; D058546:Class III Phosphatidylinositol 3-Kinases / Q000378:metabolism; D004721:Endoplasmic Reticulum / Q000201:enzymology / Q000502:physiology; D011992:Endosomes / Q000201:enzymology / Q000502:physiology; D006801:Humans; D008247:Lysosomes / Q000201:enzymology / Q000502:physiology; D021962:Membrane Microdomains / Q000201:enzymology / Q000502:physiology*; D008954:Models, Biological; D001678:Organelle Biogenesis; D018129:Phosphatidylinositol Phosphates / Q000378:metabolism*; D015290:Second Messenger Systems
chemical_list: C539000:ATG14 protein, human; D033942:Adaptor Proteins, Vesicular Transport; D000071183:Autophagy-Related Proteins; D000071186:Beclin-1; D018129:Phosphatidylinositol Phosphates; C055525:phosphatidylinositol 3-phosphate; C113567:phosphatidylinositol 5-phosphate; D058541:Class II Phosphatidylinositol 3-Kinases; D058546:Class III Phosphatidylinositol 3-Kinases
keywords: PI3P; autophagosome; endosome; lysosome; membrane trafficking