title: EPR Imaging Spin Probe Trityl Radical OX063: A Method for Its Isolation from Animal Effluent, Redox Chemistry of Its Quinone Methide Oxidation Product, and in Vivo Application in a Mouse.
abstract: We report herein a method for the recovery, purification, and application of OX063, a costly, commercially available nontoxic spin probe widely used for electron paramagnetic resonance (EPR) imaging, as well as its corresponding quinone methide (QM) form. This precious probe can be successfully recovered after use in animal model experiments (25-47% recovery from crude lyophilizate with 98.5% purity), even from samples that are >2 years old. Significantly, the recovered trityl can be reused in further animal model EPR imaging experiments. The work also describes support for the observed formation of an air-sensitive radical derived from the QM under reducing conditions.
authors.affiliation: Department of Chemistry, University of Chicago , 5735 South Ellis Avenue, Chicago, Illinois 60637, United States.
authors.forename: Maciej
authors.identifier: 0000-0003-4926-5782
authors.initials: M
authors.lastname: Serda
authors.affiliation: Department of Chemistry, University of Chicago , 5735 South Ellis Avenue, Chicago, Illinois 60637, United States.
authors.forename: Yen-Ku
authors.initials: YK
authors.lastname: Wu
authors.affiliation: The Center for EPR Imaging In Vivo Physiology, Department of Radiation and Cellular Oncology, University of Chicago , 5841 South Maryland Avenue, Chicago, Illinois 60637, United States.
authors.forename: Eugene D
authors.initials: ED
authors.lastname: Barth
authors.affiliation: The Center for EPR Imaging In Vivo Physiology, Department of Radiation and Cellular Oncology, University of Chicago , 5841 South Maryland Avenue, Chicago, Illinois 60637, United States.
authors.forename: Howard J
authors.initials: HJ
authors.lastname: Halpern
authors.affiliation: Department of Chemistry, University of Chicago , 5735 South Ellis Avenue, Chicago, Illinois 60637, United States.
authors.forename: Viresh H
authors.initials: VH
authors.lastname: Rawal
mesh_terms: D000818:Animals; D004578:Electron Spin Resonance Spectroscopy; D045563:Indolequinones / Q000737:chemistry*; D051379:Mice; D010084:Oxidation-Reduction; D013113:Spin Labels; D014316:Tritium / Q000737:chemistry*
chemical_list: D045563:Indolequinones; D013113:Spin Labels; D014316:Tritium; C068040:quinone methide