title: Acid retention with reduced glomerular filtration rate increases urine biomarkers of kidney and bone injury.
abstract: Diets high in acid of developed societies that do not cause metabolic acidosis in patients with chronic kidney disease nevertheless appear to cause acid retention with associated morbidity, particularly in those with reduced glomerular filtration rate. Here we used a rat 2/3 nephrectomy model of chronic kidney disease to study induction and maintenance of acid retention and its consequences on indicators of kidney and bone injury. Dietary acid was increased in animals eating base-producing soy protein with acid-producing casein and in casein-eating animals with added ammonium chloride. Using microdialysis to measure the kidney cortical acid content, we found that nephrectomized animals had greater acid retention than sham-operated animals when both ate the soy diet. Each increment in dietary acid further increased acid retention more in nephrectomized than in sham rats. Nephrectomized and sham animals achieved similar steady-state daily urine net acid excretion in response to increments in dietary acid but nephrectomized animals took longer to do so, contributing to greater acid retention that was maintained until the increased dietary acid was stopped. Acid retention was associated with increased urine excretion of both N-acetyl-β-D-glucosaminidase and deoxypyridinoline, greater in nephrectomized than control rats, consistent with kidney tubulointerstitial and bone matrix injury, respectively. Greater acid retention in nephrectomized than control animals was induced by a slower increase in urinary net acid excretion rate in response to the increment in dietary acid and also maintained until the dietary acid increment was stopped. Thus, acid retention increased biomarkers of kidney and bone injury in the urine, supporting untoward consequences to these two tissues.
authors.affiliation: Baylor Scott and White Health, Dallas, TX, USA; Department of Medicine, Texas A&M College of Medicine, Temple, TX, USA. Electronic address: Donald.Wesson@BSWHealth.org.
authors.forename: Donald E
authors.initials: DE
authors.lastname: Wesson
authors.affiliation: Department of Cardiovascular and Thoracic Surgery, University of Texas Southwestern Medical Center, Dallas, TX, USA.
authors.forename: Jessica
authors.initials: J
authors.lastname: Pruszynski
authors.affiliation: Department of Biostatistics, Baylor Scott and White Health, Temple, TX, USA.
authors.forename: Wendy
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Surgery, Texas Tech University Health Sciences Center, Lubbock, TX, USA.
authors.forename: Jan
authors.initials: J
authors.lastname: Simoni
mesh_terms: D000118:Acetylglucosaminidase / Q000652:urine; D000143:Acids / Q000378:metabolism*; D000468:Alkalies / Q000378:metabolism; D000596:Amino Acids / Q000652:urine; D000821:Animal Feed; D000818:Animals; D015415:Biomarkers / Q000097:blood; D001851:Bone Diseases, Metabolic / Q000209:etiology / Q000503:physiopathology* / Q000652:urine; D016723:Bone Remodeling; D002364:Caseins / Q000008:administration & dosage / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005919:Glomerular Filtration Rate; D006863:Hydrogen-Ion Concentration; D007668:Kidney / Q000503:physiopathology*; D008297:Male; D009392:Nephrectomy; D017208:Rats, Wistar; D051436:Renal Insufficiency, Chronic / Q000209:etiology / Q000503:physiopathology* / Q000652:urine; D030262:Soybean Proteins / Q000008:administration & dosage / Q000378:metabolism; D013997:Time Factors
chemical_list: D000143:Acids; D000468:Alkalies; D000596:Amino Acids; D015415:Biomarkers; D002364:Caseins; D030262:Soybean Proteins; C036020:deoxypyridinoline; D000118:Acetylglucosaminidase
keywords: acid excretion; bone; casein; chronic kidney disease; dietary protein; soy