title: Histologic changes of mesenchymal stem cell repair of tympanic membrane perforation.
abstract: CONCLUSIONS
  Mesenchymal stem-cells are good candidates for cell-therapy of chronic tympanic membranes perforations.
  
  
  OBJECTIVE
  To determine the effects of cell-based therapy in tympanic membrane perforations.
  
  
  METHODS
  Young C57BL/6 mice were anesthetized with intraperitoneal administration of ketamine and xylazine and randomly divided into three groups (n = 4 ears/group) that underwent bilateral sub-total pars tensa perforations of equal sizes using a sterile 27-gauge needle under a surgical microscope. Six-to-eight hours after injury, one group of mice did not receive treatment (acute perforation control), and the last two groups were treated with BM-MSCs embedded within HA scaffolds previously soaked in PBS to rinse culture media residues to avoid confounders and were euthanized 1 or 2 weeks after treatment.
  
  
  RESULTS
  Untreated tympanic membrane perforations developed a hyper-cellular infiltrate surrounding the injury site, while BM-MSC treated eardrums showed a reduced inflammatory response after the first week and a restoration of the trilaminar configuration 2 weeks after treatment, mimicking a normal tympanic membrane.
authors.affiliation: a Department of Otolaryngology, Head and Neck Surgery , Jackson Memorial Hospital , Miami , FL , USA.
authors.forename: Stefania
authors.initials: S
authors.lastname: Goncalves
authors.affiliation: a Department of Otolaryngology, Head and Neck Surgery , Jackson Memorial Hospital , Miami , FL , USA.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Bas
authors.affiliation: a Department of Otolaryngology, Head and Neck Surgery , Jackson Memorial Hospital , Miami , FL , USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Langston
authors.affiliation: a Department of Otolaryngology, Head and Neck Surgery , Jackson Memorial Hospital , Miami , FL , USA.
authors.forename: Ariel
authors.initials: A
authors.lastname: Grobman
authors.affiliation: a Department of Otolaryngology, Head and Neck Surgery , Jackson Memorial Hospital , Miami , FL , USA.|b Department of Otolaryngology , Interdisciplinary Stem Cell Institute, University of Miami Miller School of Medicine, University of Miami Ear Institute , Miami , FL , USA.
authors.forename: Bradley J
authors.initials: BJ
authors.lastname: Goldstein
authors.affiliation: a Department of Otolaryngology, Head and Neck Surgery , Jackson Memorial Hospital , Miami , FL , USA.
authors.forename: Simon
authors.initials: S
authors.lastname: Angeli
mesh_terms: D000818:Animals; D045164:Mesenchymal Stem Cell Transplantation; D018058:Tympanic Membrane Perforation / Q000628:therapy*
keywords: Tympanic membrane; chronic tympanic membrane perforations; stem cells; wound healing