title: Haemato-biochemical, non-specific immunity, antioxidant capacity and histopathological changes in Labeo rohita fingerlings fed rubber protein isolate.
abstract: A 60-day feeding trial was conducted to evaluate the haemato-biochemical, innate immune response, antioxidant capacity and histopathological changes in Labeo rohita fingerlings fed rubber protein isolates (RPI). One hundred and eighty fingerlings (average weight 4.45 ± 0.01 g) were distributed into five experimental groups in triplicate and fed with isonitrogenous and isocaloric diets. Soybean protein isolate (SPI) served as the reference diet (Control), and the treatment diets were formulated as RPI25, RPI50, RPI75and RPI100replacing 25, 50, 75 and 100% of SPI protein, respectively. The growth performance indices like final body weight (9.54-10.27 g), net weight gain (5.09-5.84 g), metabolic growth rate (4.54-5.02) and feed efficiency ratio (0.60-0.65) among the various groups were not significantly different (P > 0.05). All the haematological parameters, except red blood cells, showed no significant differences compared with the control group (P > 0.05). The immuno-biochemical parameters like albumin, globulin, total immunoglobulin, respiratory burst and lysozyme activities among the various groups did not differ significantly (P > 0.05). The stress enzyme such as superoxide dismutase (SOD), catalase (CAT) and oxidative stress marker malondialdehyde (MDA) showed no significant difference (P > 0.05). Histopathological examination of the liver revealed no marked changes. In summary, the results showed that RPI was well utilised by the fish and its inclusion did not generate any oxidative-induced stress, thus, RPI may be suggested as a potential replacement for SPI in fish diets without any detrimental effects. Hence, protein isolation offers a unique opportunity for the utilisation of rubber seed meal.
authors.affiliation: Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Mumbai, India. phem247@yahoo.com.|Department of Biological Sciences, Wesley University, Ondo, Ondo State, Nigeria. phem247@yahoo.com.
authors.forename: Femi John
authors.initials: FJ
authors.lastname: Fawole
authors.affiliation: Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Mumbai, India. npsahu@cife.edu.in.
authors.forename: N P
authors.initials: NP
authors.lastname: Sahu
authors.affiliation: Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: K K
authors.initials: KK
authors.lastname: Jain
authors.affiliation: Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: S
authors.initials: S
authors.lastname: Gupta
authors.affiliation: Aquatic Animal Health Division, ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: K V
authors.initials: KV
authors.lastname: Rajendran
authors.affiliation: Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: N
authors.initials: N
authors.lastname: Shamna
authors.affiliation: Aquatic Animal Health Division, ICAR-Central Institute of Fisheries Education, Mumbai, India.
authors.forename: Nalini
authors.initials: N
authors.lastname: Poojary
mesh_terms: D000821:Animal Feed / Q000032:analysis*; D000824:Animal Nutritional Physiological Phenomena; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D003530:Cyprinidae / Q000097:blood / Q000276:immunology / Q000502:physiology*; D004032:Diet / Q000662:veterinary*; D004044:Dietary Proteins / Q000032:analysis*; D004906:Erythrocyte Count; D004909:Erythrocyte Indices; D006400:Hematocrit; D007113:Immunity, Innate; D007958:Leukocyte Count; D018384:Oxidative Stress
chemical_list: D000975:Antioxidants; D004044:Dietary Proteins
keywords: Haematology; Innate immunity; Labeo rohita; Oxidative enzyme; Protein isolate