title: Local Anesthetic Activity from Extracts, Fractions and Pure Compounds from the Roots of Ottonia anisum Spreng. (Piperaceae).
abstract: Piperaceae species can be found worldwide in tropical and subtropical areas and many of them have been used for centuries in traditional folk medicine and in culinary. In Brazil, species of Piperaceae are commonly used in some communities as local anesthetic and analgesic. Countrified communities have known some species of the genus Ottonia as "anestesia" and it is a common habit of chewing leaves and roots of Ottonia species to relief toothache. The purpose of this study is to report our findings on new molecules entities obtained from the roots of Ottonia anisum Spreng, in which local anesthetic activity (sensory blockage) is demonstrated for the first time in vivo guinea pig model. Phytochemical investigation led to the isolation of three amides (pipercallosidine, piperine and valeramide) and in an enriched mixture of seven amides (valeramide, 4,5-dihydropiperlonguminine, N-isobutil-6-piperonil-2-hexenamide, piperovatine, dihydropipercallosidine, pipercallosidine and pipercallpsine). Our findings demonstrated the anesthetic potential for the methanolic extract from roots, its n-hexane partition and amides from O. anisum and it is in agreement with ethnobotanical survey.
authors.affiliation: Programa de Pesquisa em Desenvolvimento de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro/UFRJ, Av. Brigadeiro Trompowski, s/n, Ilha do Fundão, 21941-902 Rio de Janeiro, RJ, Brazil.
authors.forename: Kelvin S E
authors.initials: KS
authors.lastname: López
authors.affiliation: Instituto de Pesquisas de Produtos Naturais/IPPN, Universidade Federal do Rio de Janeiro/UFRJ, Av. Carlos Chagas Filho, 373, 21941-902 Rio de Janeiro, RJ, Brazil.
authors.forename: André M
authors.initials: AM
authors.lastname: Marques
authors.affiliation: Laboratório de Produtos Naturais, PN5, Far-Manguinhos, FIOCRUZ, Rua Sizenando Nabuco, 100, Manguinhos, 21041-250 Rio de Janeiro, RJ, Brazil.
authors.forename: Davyson DE L
authors.initials: DL
authors.lastname: Moreira
authors.affiliation: Instituto de Pesquisas de Produtos Naturais/IPPN, Universidade Federal do Rio de Janeiro/UFRJ, Av. Carlos Chagas Filho, 373, 21941-902 Rio de Janeiro, RJ, Brazil.
authors.forename: Leosvaldo S
authors.initials: LS
authors.lastname: Velozo
authors.affiliation: Programa de Pesquisa em Desenvolvimento de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro/UFRJ, Av. Brigadeiro Trompowski, s/n, Ilha do Fundão, 21941-902 Rio de Janeiro, RJ, Brazil.
authors.forename: Roberto T
authors.initials: RT
authors.lastname: Sudo
authors.affiliation: Programa de Pesquisa em Desenvolvimento de Fármacos, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro/UFRJ, Av. Brigadeiro Trompowski, s/n, Ilha do Fundão, 21941-902 Rio de Janeiro, RJ, Brazil.
authors.forename: Gisele
authors.initials: G
authors.lastname: Zapata-Sudo
authors.affiliation: Instituto de Pesquisas Jardim Botânico do Rio de Janeiro, Rua Pacheco Leão, 915, Jardim Botânico, 2240-030 Rio de Janeiro, RJ, Brazil.
authors.forename: Elsie F
authors.initials: EF
authors.lastname: Guimarães
authors.affiliation: Instituto de Pesquisas de Produtos Naturais/IPPN, Universidade Federal do Rio de Janeiro/UFRJ, Av. Carlos Chagas Filho, 373, 21941-902 Rio de Janeiro, RJ, Brazil.
authors.forename: Maria Auxiliadora C
authors.initials: MA
authors.lastname: Kaplan
mesh_terms: D000577:Amides / Q000302:isolation & purification*; D000779:Anesthetics, Local / Q000302:isolation & purification*; D000818:Animals; D052117:Benzodioxoles / Q000302:isolation & purification; D001938:Brazil; D006168:Guinea Pigs; D006586:Hexanes / Q000302:isolation & purification; D026321:Piperaceae / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry*; D018517:Plant Roots / Q000737:chemistry*
chemical_list: C521746:4,5-dihydropiperlonguminine; D000577:Amides; D000779:Anesthetics, Local; D052117:Benzodioxoles; D006586:Hexanes; D010936:Plant Extracts; C026385:n-hexane