title: High-resolution mass spectrometry-based global proteomic analysis of probiotic strains Lactobacillus fermentum NCDC 400 and RS2.
abstract: Lactobacillus fermentum strains NCDC 400 and RS2, previously isolated from dairy sources, exhibited excellent probiotic properties were studied for the global proteomic profile. A total of 1125 proteins were identified by a high-resolution mass spectrometer, ESI-qTOF (nano-LC-MS/MS) in the strains of L. fermentum. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis resulted in 60.9% and 59.2% of the total proteins were functionally annotated for NCDC 400 and RS2 respectively. Simultaneously, a cluster of orthologous groups (COGs) and KEGG together revealed the presence of a significant number of proteins involved in transcription, translation, chaperones, peptidoglycan biosynthesis, nucleotide, amino acid and carbohydrate metabolism. Most of the proteins that play a vital role in the formation of RNA polymerase, ribosomal subunits, and aminoacyl-tRNA biosynthesis were fully mapped. Further analysis by bioinformatics tools revealed that 13.83% of the proteins were hydrophobic while 86.17% were hydrophilic in nature. The present findings represent the first draft proteome map of L. fermentum strains and demonstrate the involvement of important proteins in normal physiology and growth of potential probiotic strain.
authors.affiliation: National Collection of Dairy Cultures (NCDC) Lab, Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Parijat
authors.initials: P
authors.lastname: Pragya
authors.affiliation: Proteomics and Cell Biology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Gurjeet
authors.initials: G
authors.lastname: Kaur
authors.affiliation: Proteomics and Cell Biology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Syed Azmal
authors.initials: SA
authors.lastname: Ali
authors.affiliation: Proteomics and Cell Biology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Shveta
authors.initials: S
authors.lastname: Bhatla
authors.affiliation: Proteomics and Cell Biology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Rawat
authors.affiliation: National Collection of Dairy Cultures (NCDC) Lab, Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Vaibhao
authors.initials: V
authors.lastname: Lule
authors.affiliation: Proteomics and Cell Biology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: Sudarshan
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Proteomics and Cell Biology Lab, Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India. Electronic address: ashokmohanty1@gmail.com.
authors.forename: Ashok Kumar
authors.initials: AK
authors.lastname: Mohanty
authors.affiliation: National Collection of Dairy Cultures (NCDC) Lab, Dairy Microbiology Division, National Dairy Research Institute, Karnal 132001, Haryana, India. Electronic address: pradip_behare@yahoo.com.
authors.forename: Pradip
authors.initials: P
authors.lastname: Behare
mesh_terms: D019881:Aminoacyltransferases; D001426:Bacterial Proteins / Q000032:analysis; D002853:Chromatography, Liquid; D012321:DNA-Directed RNA Polymerases; D057927:Hydrophobic and Hydrophilic Interactions; D052197:Limosilactobacillus fermentum / Q000737:chemistry* / Q000254:growth & development; D019936:Probiotics / Q000737:chemistry*; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods*; D054657:Ribosome Subunits; D053719:Tandem Mass Spectrometry
chemical_list: D001426:Bacterial Proteins; D020543:Proteome; D019881:Aminoacyltransferases; D012321:DNA-Directed RNA Polymerases
keywords: Lactobacillus fermentum; Nano-LC-MS/MS; Pathway analysis; Probiotic; Proteomic profiling