title: Chemical Constituents, Antimicrobial, Cytotoxicity, Mutagenic and Antimutagenic Effects ofArtemisia ciniformis.
abstract: The aim of this study was to determine the chemical constituents, antimicrobial, cytotoxicity, mutagenic and anti-mutagenic activities of the essential oil ofArtemisia ciniformisKrasch. & Popov ex Poljakov, against important bacterial pathogens and human cells which were unknown before.In-vitrocytotoxicity was measured using a modified MTT assay on normal human lymphocytes and tumor HeLa cells. The mutagenic and antimutagenic activities of the oil were evaluated using theSalmonellatyphimuriumtester strains TA98 and TA100, together with nitrofluorene for TA98 and sodium azide for TA100 without (-S9) metabolic activation, and 2-aminoantracene for TA98 and TA100 with metabolic (+S9) activation. Oxygenated monoterpenes especially camphor (30.21%), 1,8-cineole (23.7%) andtrans-Pinocarveol (12.28%) were the major components of the oil ofA. ciniformis.Bactericidal kinetics of this oil indicated thatAcinetobacter baumanniiis the most vulnerable one (MIC = 0.02 mg/mL, MBC = 0.04 mg/mL, Dvalue= 3.57 min). The oil displayed an excellent cytotoxic action toward the human tumor cell line (IC50= 19.64 µg/mL). The oil ofA. ciniformisshowed excellent antimutagenicity effect on the 2-nitrofluorene, in the strain ofS. typhimuriumTA98, without the presence of metabolic activation.
authors.affiliation: Department of Chemistry, College of Science, Takestan Branch, Islamic Azad University, Takestan, Iran.
authors.forename: Mahboubeh
authors.initials: M
authors.lastname: Taherkhani
keywords: Antimicrobial; Antimutagenic; Artemisia ciniformis; Cytotoxicity; HeLa and lymphocytes cells