title: ERF73/HRE1 is involved in H2O2production via hypoxia-inducible Rboh gene expression in hypoxia signaling.
abstract: Hypoxia deprives cells of energy and induces severe physical damage in embryophytes. Under hypoxia, the equilibrium between ethylene and H2O2affects the response of the transcription factor AtERF73/HRE1. To evaluate the role of AtERF73/HRE1 during hypoxia signaling, we used three independent AtERF73/HRE1 knockout lines to detect H2O2accumulation. The results revealed that under hypoxia, H2O2accumulation in the AtERF73/HRE1 knockout lines decreased, indicating that AtERF73/HRE1 uses a negative feedback regulation mechanism to influence the production of H2O2induced through hypoxia signal transduction. Quantitative RT-PCR analyses showed that oxygen deficiency had different effects on the expression of the hypoxia-induced genes Rboh B, D, G, and I in the AtERF73/HRE1 knockout lines. In particular, Rboh B and D expression were increased, whereas Rboh G expression was decreased. The expression of Rboh I was increased at 1 h but decreased at 3 h during hypoxia treatment in the AtERF73/HRE1 knockout lines. Similarly, the transcript levels of antioxidant and hypoxia-induced/ethylene response genes in the AtERF73/HRE1 knockout lines were affected by hypoxic stress, indicating that AtERF73/HRE1 is essential to hypoxia signal transduction in embryophytes. Additionally, in histochemical analysis, AtERF73/HRE1 promoter-induced GUS expression was detected in various plant parts throughout the plant growth process (e.g., leaves, inflorescences, siliques), particularly in the edges of mature leaves and guard cells. Taken together, our results confirm that AtERF73/HRE1 plays a role in H2O2production by affecting the hypoxia-induced expression of Rboh genes in hypoxia signal transduction.
authors.affiliation: Department of Agronomy, National Chung Hsing University, Taichung, 40227, Taiwan. emiyang@dragon.nchu.edu.tw.
authors.forename: Chin-Ying
authors.initials: CY
authors.lastname: Yang
authors.affiliation: Department of Agronomy, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Yi-Chun
authors.initials: YC
authors.lastname: Huang
authors.affiliation: Department of Agronomy, National Chung Hsing University, Taichung, 40227, Taiwan.
authors.forename: Shang-Ling
authors.initials: SL
authors.lastname: Ou
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000502:physiology*; D015687:Cell Hypoxia; D035264:Flowers / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism*; D019255:NADPH Oxidases / Q000235:genetics* / Q000378:metabolism; D011401:Promoter Regions, Genetic; D036226:Seedlings / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D015534:Trans-Activators / Q000502:physiology*; D015533:Transcriptional Activation
chemical_list: D029681:Arabidopsis Proteins; C000596383:HRE1 protein, Arabidopsis; D015534:Trans-Activators; D006861:Hydrogen Peroxide; D019255:NADPH Oxidases
keywords: Antioxidant; AtERF73/HRE1; Hydrogen peroxide; Hypoxia; Rboh