title: Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans.
abstract: The molecular mechanisms underlying endometrial stromal cell proliferation and differentiation (decidualization) are still not fully understood. This study revealed that increased Slp-2 expression is a significant factor modulating endometrial stromal cell proliferation and decidualization in both mice and humans. Our results showed a significant difference in the mRNA and protein levels between the implantation site and inter-implantation site on day 5 and day 6 of pregnancy in mice (all P < 0.05). Strong Slp-2 immunostaining was mainly localized within the decidual zone of mice through the post-implantation period. Mice with artificially induced deciduoma showed significantly higher expression of Slp-2 compared with uninduced controls (P < 0.005). Human stromal cells in the middle and late-secretory phases demonstrated significantly (all P < 0.05) upregulated SLP-2, compared with cells in the proliferative phase and early secretory phases. Further analyses of the SLP-2 gene knocked down revealed a significant (P < 0.005) repression of both the decidualization marker gene's expression (decidual/trophoblast prolactin-related protein in mice, insulin-like growth factor binding protein and prolactin in human) and the cell proliferation in in vitro-induced decidualized primary endometrial stromal cells in mice and humans.
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Department of Obstetrics and Gynaecology, The First Affiliated Hospital, Chongqing Medical University, Building 5 Room 507, No. 1 Youyi Rd, Chongqing 400016, China.
authors.forename: Zhuo-Ying
authors.initials: ZY
authors.lastname: Hu
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Xue-Qing
authors.initials: XQ
authors.lastname: Liu
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Lan
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Geng
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Xue-Mei
authors.initials: XM
authors.lastname: Chen
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Jun-Lin
authors.initials: JL
authors.lastname: He
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China.
authors.forename: Ying-Xiong
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Laboratory of Reproductive Biology, School of Public Health and Management, Chongqing Medical University, Box 197, No. 1 Yixueyuan Rd, Chongqing 400016, China. Electronic address: dingyb@gmail.com.
authors.forename: Yu-Bin
authors.initials: YB
authors.lastname: Ding
mesh_terms: D000818:Animals; D001798:Blood Proteins / Q000378:metabolism*; D002454:Cell Differentiation; D049109:Cell Proliferation; D003656:Decidua / Q000378:metabolism; D033301:Deciduoma / Q000378:metabolism; D010064:Embryo Implantation; D004717:Endometrium / Q000166:cytology*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D018970:Insulin-Like Growth Factor Binding Protein 1 / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism*; D008597:Menstrual Cycle; D051379:Mice; D011247:Pregnancy; D011388:Prolactin / Q000031:analogs & derivatives / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism
chemical_list: D001798:Blood Proteins; C489796:Dtprp protein, mouse; C032231:IGFBP1 protein, human; D018970:Insulin-Like Growth Factor Binding Protein 1; D008565:Membrane Proteins; D012333:RNA, Messenger; C406287:STOML2 protein, human; C424134:Sytl2 protein, mouse; D011388:Prolactin
keywords: SLP-2; decidualization; embryo implantation; stromal cells