title: miR-760 mediates chemoresistance through inhibition of epithelial mesenchymal transition in breast cancer cells.
abstract: Therapeutic resistance has been a great obstacle for successful treatment of breast cancer. Our study aimed to explore the role of microRNA-760 (miR-760) in chemoresistant breast cancer cells.
  
  
  
  Real-time PCR was performed to measure the mRNA expression of miR-760 and Nanog. Western blot was used to determine the protein expression of Nanog and mesenchymal and epithelial markers. Cell viability was measured by the CCK-8 assay.
  
  
  
  Our results showed that the expression of miR-760 was significantly reduced the doxorubicin (DOX)-resistant MCF-7/DOX cells and chemoresistant breast cancer tissues. Moreover, up-regulation of miR-760 sensitized breast cancer cells to the anti-cancer agents. The MCF-7/DOX cells exhibited increased expression of Snail, a mesenchymal marker, and decreased levels of E-Cadherin, an epithelial marker. In addition, overexpression of miR-760 suppressed the expression of Nanog, a transcriptional factor involved in chemoresistance, and resulted in the reversal of EMT in breast cancer cells.
  
  
  
  Our study demonstrated that miR-760 modulated chemoresistance through the epithelial-mesenchymal transition in breast cancer cells, providing a potential therapeutic target for treatment of drug-resistant breast cancer.
authors.affiliation: Department of Clinical Laboratory, Affiliated Hospital of Dali University, Dali, China. cguoqing@yeah.net.
authors.forename: S-H
authors.initials: SH
authors.lastname: Hu
authors.forename: C-H
authors.initials: CH
authors.lastname: Wang
authors.forename: Z-J
authors.initials: ZJ
authors.lastname: Huang
authors.forename: F
authors.initials: F
authors.lastname: Liu
authors.forename: C-W
authors.initials: CW
authors.lastname: Xu
authors.forename: X-L
authors.initials: XL
authors.lastname: Li
authors.forename: G-Q
authors.initials: GQ
authors.lastname: Chen
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics; D004317:Doxorubicin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics
chemical_list: C579293:MIRN760 microRNA, human; D035683:MicroRNAs; D004317:Doxorubicin