title: Comparative Antibody Responses Against three Antimalarial Vaccine Candidate Antigens from Urban and Rural Exposed Individuals in Gabon.
abstract: The analysis of immune responses in diverse malaria endemic regions provides more information to understand the host's immune response toPlasmodium falciparum.Several plasmodial antigens have been reported as targets of human immunity. PfAMA1 is one of most studied vaccine candidates; PfRH5 and Pf113 are new promising vaccine candidates. The aim of this study was to evaluate humoral response against these three antigens among children of Lastourville (rural area) and Franceville (urban area). Malaria was diagnosed using rapid diagnosis tests. Plasma samples were tested against these antigens by enzyme-linked immunosorbent assay (ELISA). We found that malaria prevalence was five times higher in the rural area than in the urban area (p< 0.0001). The anti-PfAMA1 and PfRh5 response levels were significantly higher in Lastourville than in Franceville (p< 0.0001;p= 0.005). The anti-AMA1 response was higher than the anti-Pf113 response, which in turn was higher than the anti-PfRh5 response in both sites. Anti-PfAMA1 levels were significantly higher in infected children than those in uninfected children (p= 0.001) in Franceville. Anti-Pf113 and anti-PfRh5 antibody levels were lowest in children presenting severe malarial anemia. These three antigens are targets of immunity in Gabon. Further studies on the role of Pf113 in antimalarial protection against severe anemia are needed.
authors.affiliation: Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), BP 769 Franceville, Gabon; Molécules de Communication et Adaptation des Microorganismes (MCAM, UMR 7245), Sorbonne Universités, Muséum National d'Histoire Naturelle, CNRS, CP52, 57 rue Cuvier 75005 Paris, France; Ecole Doctorale Régionale en Infectiologie Tropicale d'Afrique Centrale (ECODRAC), BP 876 Franceville, Gabon.
authors.forename: Roméo-Karl
authors.initials: RK
authors.lastname: Imboumy-Limoukou
authors.affiliation: Laboratoire de Recherches en Immunologie, Parasitologie et Microbiologie, Ecole Doctorale Régionale en Infectiologie Tropicale d'Afrique Centrale (ECODRAC) , BP 876 Franceville, Gabon.
authors.forename: Sandrine Lydie
authors.initials: SL
authors.lastname: Oyegue-Liabagui
authors.affiliation: Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF) , BP 769 Franceville, Gabon.
authors.forename: Stella
authors.initials: S
authors.lastname: Ndidi
authors.affiliation: Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), BP 769 Franceville, Gabon; Ecole Doctorale Régionale en Infectiologie Tropicale d'Afrique Centrale (ECODRAC), BP 876 Franceville, Gabon.
authors.forename: Irène
authors.initials: I
authors.lastname: Pegha-Moukandja
authors.affiliation: Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF) , BP 769 Franceville, Gabon.
authors.forename: Charlene Lady
authors.initials: CL
authors.lastname: Kouna
authors.affiliation: Wellcome Trust Sanger Institute , Cambridge CB10 1HH, UK.
authors.forename: Francis
authors.initials: F
authors.lastname: Galaway
authors.affiliation: Molécules de Communication et Adaptation des Microorganismes (MCAM, UMR 7245), Sorbonne Universités, Muséum National d'Histoire Naturelle, CNRS, CP52 , 57 rue Cuvier 75005 Paris, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Florent
authors.affiliation: Unité de Parasitologie Médicale (UPARAM), Centre International de Recherches Médicales de Franceville (CIRMF), BP 769 Franceville, Gabon; Département de Parasitologie-Mycologie, Université des Sciences de la Santé, BP 4008 Libreville, Gabon.
authors.forename: Jean Bernard
authors.initials: JB
authors.lastname: Lekana-Douki
keywords: Gabon; P. falciparum; Pf AMA1; Pf113; PfRh5; antibodies; vaccine candidates