title: Effects of Contamination by Hemostatic Agents and Use of Cleaning Agent on Etch-and-Rinse Dentin Bond Strength.
abstract: The aim of this study was to evaluate the influence of contamination by hemostatic agents and rinsing with chlorhexidine on bond strength between dentin and resin composite. Ninety-six bovine teeth were sectioned to expose a flat dentin surface area. A standardized cavity with 2.0 mm in thickness, superficial diameter of 4.0 mm and bottom diameter of 3.0 mm was prepared with a diamond bur in each dentin specimen. The teeth were divided into four groups according to the hemostatic employed: G1: control; G2: use of ViscoStat, Ultradent; G3: Hemosthasegel, FGM; and G4: Hemostop, Dentsply. The groups were divided into two subgroups according to the cleaning protocol method (n=12): A: without any further cleaning; and B: cleaning with chlorhexidine at 0.2%. All cavities were filled using a dentin adhesive and a resin composite, following the manufacturer's instructions. After 24 h, the specimens were aged by thermal and mechanical cycling. The bond strength was determined by the push out bond test (MPa), Statistical analysis was performed using two-way ANOVA and Tukey test (p<0.05). Statistically significant differences were detected among all groups treated with hemostatic agents and the control group. The post-hoc test showed that cleaning the cavity with chlorhexidine significantly improves the bond strength between dentin and resin composite. Our results suggested that the use of chlorhexidine can reestablish the bond strength between dentin and resin composite when a hemostatic agent was applied.
authors.affiliation: Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista,São José dos Campos, SP, Brazil.
authors.forename: César Rogério
authors.initials: CR
authors.lastname: Pucci
authors.affiliation: Department of Dental Materials and Prosthodontics, Institute of Science and Technology, UNESP - Univ Estadual Paulista, São José dos Campos, SP, Brazil.
authors.forename: Rodrigo Maximo de
authors.initials: RM
authors.lastname: Araújo
authors.affiliation: Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista,São José dos Campos, SP, Brazil.
authors.forename: Ana Julia Farias de
authors.initials: AJ
authors.lastname: Lacerda
authors.affiliation: Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista,São José dos Campos, SP, Brazil.
authors.forename: Mirella Anjos de
authors.initials: MA
authors.lastname: Souza
authors.affiliation: Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista,São José dos Campos, SP, Brazil.
authors.forename: Maria Filomena Rocha Lima
authors.initials: MF
authors.lastname: Huhtala
authors.affiliation: Department of Restorative Dentistry, Institute of Science and Technology, UNESP - Univ Estadual Paulista,São José dos Campos, SP, Brazil.
authors.forename: Fernanda Alves
authors.initials: FA
authors.lastname: Feitosa
mesh_terms: D000818:Animals; D002417:Cattle; D001840:Dental Bonding; D004202:Disinfectants; D006490:Hemostatics / Q000009:adverse effects / Q000494:pharmacology*; D066298:In Vitro Techniques
chemical_list: D004202:Disinfectants; D006490:Hemostatics