title: Laser Light-field Fusion for Wide-field Lensfree On-chip Phase Contrast Microscopy of Nanoparticles.
abstract: Wide-field lensfree on-chip microscopy, which leverages holography principles to capture interferometric light-field encodings without lenses, is an emerging imaging modality with widespread interest given the large field-of-view compared to lens-based techniques. In this study, we introduce the idea of laser light-field fusion for lensfree on-chip phase contrast microscopy for detecting nanoparticles, where interferometric laser light-field encodings acquired using a lensfree, on-chip setup with laser pulsations at different wavelengths are fused to produce marker-free phase contrast images of particles at the nanometer scale. As a proof of concept, we demonstrate, for the first time, a wide-field lensfree on-chip instrument successfully detecting 300 nm particles across a large field-of-view of ~30 mm2without any specialized or intricate sample preparation, or the use of synthetic aperture- or shift-based techniques.
authors.affiliation: Department of Systems Design Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Farnoud
authors.initials: F
authors.lastname: Kazemzadeh
authors.affiliation: Department of Systems Design Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada.
authors.forename: Alexander
authors.initials: A
authors.lastname: Wong