title: Biochemical Characterization of a Eukaryotic Decalin-Forming Diels-Alderase.
abstract: The trans-decalin structure formed by intramolecular Diels-Alder cycloaddition is widely present among bioactive natural products isolated from fungi. We elucidated the concise three-enzyme biosynthetic pathway of the cytotoxic myceliothermophin and biochemically characterized the Diels-Alderase that catalyzes the formation of trans-decalin from an acyclic substrate. Computational studies of the reaction mechanism rationalize both the substrate and stereoselectivity of the enzyme.
authors.affiliation: Engineering Research Center of Industrial Microbiology (Ministry of Education) and College of Life Sciences, Fujian Normal University , Fuzhou 350117, P. R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.forename: Peiyuan
authors.initials: P
authors.lastname: Yu
authors.forename: Man-Cheng
authors.initials: MC
authors.lastname: Tang
authors.forename: Yi
authors.initials: Y
authors.lastname: Zou
authors.forename: Shu-Shan
authors.initials: SS
authors.lastname: Gao
authors.forename: Yiu-Sun
authors.initials: YS
authors.lastname: Hung
authors.forename: Muxun
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutical Sciences, University of Shizuoka , Shizuoka 422-8526, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Watanabe
authors.forename: K N
authors.initials: KN
authors.lastname: Houk
authors.forename: Yi
authors.initials: Y
authors.lastname: Tang
mesh_terms: D055162:Biocatalysis; D061565:Cycloaddition Reaction; D056890:Eukaryota / Q000737:chemistry* / Q000378:metabolism; D019605:Euryarchaeota / Q000201:enzymology; D009281:Naphthalenes / Q000737:chemistry / Q000378:metabolism*; D010453:Peptide Synthases / Q000737:chemistry / Q000378:metabolism*; D048630:Polyketide Synthases / Q000737:chemistry / Q000378:metabolism*
chemical_list: D009281:Naphthalenes; D048630:Polyketide Synthases; C007229:decalin; D010453:Peptide Synthases; C104385:non-ribosomal peptide synthase