title: Detection ofPorphyromonas gingivalisandTreponema denticolain chronic and aggressive periodontitis patients: A comparative polymerase chain reaction study.
abstract: BACKGROUND
  The detection frequency ofPorphyromonas gingivalisandTreponema denticolain chronic periodontitis (CP) and aggressive periodontitis (AgP) is not explored well in Indian population.
  
  
  OBJECTIVE
  The study was undertaken to detectP. gingivalisandT. denticolain CP as well as in AgP patients using polymerase chain reaction (PCR), and to determine the relationship between the frequency of these two microorganisms and the severity of clinical periodontal parameters.
  
  
  METHODS
  Subgingival plaque samples were collected from ninety participants (thirty CP patients, thirty AgP patients, and thirty healthy participants) and the aforementioned two microorganisms were detected using PCR.
  
  
  RESULTS
  However, when CP and AgP were compared for the detection frequency of two microorganisms, no statistically significant difference was noted. A statistically significant increase in the number of bacteria-positive sites increased as the score of plaque index (PI), gingival index (GI), and clinical attachment level of CP and AgP patients increased. Coexistence ofP. gingivalisandT. denticolawas frequently observed in deep periodontal pockets.
  
  
  CONCLUSIONS
  Study findings suggest thatP. gingivalisandT. denticolaare significantly associated with the severity of periodontal tissue destruction. Statistically significant association exists between clinical periodontal parameters such as PI, GI, periodontal pocket depth (PPD), and clinical attachment loss and presence of both the microorganisms.
authors.affiliation: Department of Oral Pathology and Microbiology, Center for Dental Education and Research, AIIMS, New Delhi, India.
authors.forename: Ramniwas M
authors.initials: RM
authors.lastname: Kumawat
authors.affiliation: Department of Oral Pathology and Microbiology, Government Dental College and Hospital, Nagpur, Maharashtra, India.
authors.forename: Sindhu M
authors.initials: SM
authors.lastname: Ganvir
authors.affiliation: Department of Oral Pathology and Microbiology, Government Dental College and Hospital, Nagpur, Maharashtra, India.
authors.forename: Vinay K
authors.initials: VK
authors.lastname: Hazarey
authors.affiliation: Environmental Genomics Division, National Environment and Engineering Research Institute, Nagpur, Maharashtra, India.
authors.forename: Asifa
authors.initials: A
authors.lastname: Qureshi
authors.affiliation: Environmental Genomics Division, National Environment and Engineering Research Institute, Nagpur, Maharashtra, India.
authors.forename: Hemant J
authors.initials: HJ
authors.lastname: Purohit
keywords: Aggressive periodontitis; Porphyromonas gingivalis; Treponema denticola; chronic periodontitis; polymerase chain reaction