title: Monosynaptic Tracing in Developing Circuits Using Modified Rabies Virus.
abstract: An attenuated rabies virus that expresses fluorescent protein has made it possible to analyze retrograde (presynaptic) monosynaptic connections in vivo. By combining attenuated rabies virus with a Cre-loxP based system to target cells in a subtype-specific fashion, it is possible to examine neuronal input in vivo onto any class of neuron, in development and in the mature brain. We describe here the methods to amplify deletion mutant, pseudotyped rabies virus, selectively target cells of interest using genetic and viral approaches, as well as the stereotaxic procedures required to target neuronal subtypes of interest in vivo.
authors.affiliation: Department of Neurology, University of California, San Francisco, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA. laura.cocas@ucsf.edu.|Department of Biological Sciences, California State University, 5151, Los Angeles, CA, 90032, USA. laura.cocas@ucsf.edu.
authors.forename: Laura
authors.initials: L
authors.lastname: Cocas
authors.affiliation: Department of Neurology, University of California, San Francisco, 675 Nelson Rising Lane, San Francisco, CA, 94158, USA.
authors.forename: Gloria
authors.initials: G
authors.lastname: Fernandez
mesh_terms: D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D063132:Connectome / Q000379:methods*; D015870:Gene Expression; D017930:Genes, Reporter; D005822:Genetic Vectors; D006378:Helper Viruses / Q000502:physiology; D051379:Mice; D008856:Microscopy, Fluorescence; D009474:Neurons / Q000502:physiology* / Q000821:virology*; D011820:Rabies virus / Q000502:physiology*; D013569:Synapses / Q000502:physiology* / Q000821:virology; D056189:Viral Tropism; D014779:Virus Replication
keywords: Monosynaptic circuit tracing; Neuronal circuit development; Rabies virus; Stereotaxic surgery; Viral amplification