title: Decreased levels of sRAGE in follicular fluid from patients with PCOS.
abstract: This study aimed to explore the association between soluble receptor for advanced glycation end products (sRAGE) levels in follicular fluid and the number of oocytes retrieved and to evaluate the effect of sRAGE on vascular endothelial growth factor (VEGF) in granulosa cells in patients with polycystic ovarian syndrome (PCOS). Two sets of experiments were performed in this study. In part one, sRAGE and VEGF protein levels in follicular fluid samples from 39 patients with PCOS and 35 non-PCOS patients were measured by ELISA. In part two, ovarian granulosa cells were isolated from an additional 10 patients with PCOS and cultured. VEGF and SP1 mRNA and protein levels, as well as pAKT levels, were detected by real-time PCR and Western blotting after cultured cells were treated with different concentrations of sRAGE. Compared with the non-PCOS patients, patients with PCOS had lower sRAGE levels in follicular fluid. Multi-adjusted regression analysis showed that high sRAGE levels in follicular fluid predicted a lower Gn dose, more oocytes retrieved, and a better IVF outcome in the non-PCOS group. Logistic regression analysis showed that higher sRAGE levels predicted favorably IVF outcomes in the non-PCOS group. Multi-adjusted regression analysis also showed that high sRAGE levels in follicular fluid predicted a lower Gn dose in the PCOS group. Treating granulosa cells isolated from patients with PCOS with recombinant sRAGE decreased VEGF and SP1 mRNA and protein expression and pAKT levels in a dose-dependent manner.
authors.affiliation: Reproductive Medical Centerthe First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China.|Reproductive Medical Centerthe Third Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China.
authors.forename: BiJun
authors.initials: B
authors.lastname: Wang
authors.affiliation: Reproductive Medical Centerthe First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Reproductive Medical Centerthe First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China.
authors.forename: QingLing
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Reproductive Medical Centerthe First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China.
authors.forename: FuLi
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Reproductive Medical Centerthe First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China.
authors.forename: MengMeng
authors.initials: M
authors.lastname: Hao
authors.affiliation: Reproductive Medical Centerthe First Affiliated Hospital of Zhengzhou University, Zhengzhou City, Henan Province, People's Republic of China 13613863710@163.com.
authors.forename: YiHong
authors.initials: Y
authors.lastname: Guo
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005307:Fertilization in Vitro; D015571:Follicular Fluid / Q000378:metabolism*; D006107:Granulosa Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D009865:Oocytes / Q000378:metabolism* / Q000473:pathology; D011085:Polycystic Ovary Syndrome / Q000378:metabolism* / Q000473:pathology; D060888:Real-Time Polymerase Chain Reaction; D000067759:Receptor for Advanced Glycation End Products / Q000235:genetics / Q000378:metabolism*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics / Q000378:metabolism
chemical_list: D000067759:Receptor for Advanced Glycation End Products; D042461:Vascular Endothelial Growth Factor A; C467484:VEGFA protein, human; C000593871:AGER protein, human