title: Effect of Tween 80 and Acetone on the Secretion,  Structure and Antioxidant Activities of Exopolysaccharides fromLentinus tigrinus.
abstract: In this study, the effects of the addition of Tween 80 and acetone on secretion, structure and antioxidant activities ofLentinus tigrinusexopolysaccharides (EPS) were investigated. It was found that Tween 80 and acetone displayed a stimulatory effect on EPS secretion. The EPS obtained by the addition of Tween 80 (EPS-T), acetone (EPS-A) and control (EPS--C) were purified by Sepharose CL-6B gel filtration chromatography and molecular mass of purified fractions was estimated to be 22.1, 137 and 12 kDa, respectively. Monosaccharide composition analysis indicated that EPS-T, EPS-A and EPS-C were mainly composed of glucose and mannose. Congo Red test indicated that EPS-T and EPS-A had a highly ordered conformation of triple helix, while EPS-C had a random coil conformation. Furthermore, EPS-A exhibited higher DPPH scavenging and antiproliferative activities than EPS--C and EPS-T, which might be attributed to the molecular mass.
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China; Collaborative Innovation Center of Food Production and Safety, Henan Province, Zhengzhou 450001, PR China.
authors.forename: Peixin
authors.initials: P
authors.lastname: He
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
authors.forename: Shuangshuang
authors.initials: S
authors.lastname: Wu
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
authors.forename: Lige
authors.initials: L
authors.lastname: Pan
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China.
authors.forename: Siwen
authors.initials: S
authors.lastname: Sun
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China; Collaborative Innovation Center of Food Production and Safety, Henan Province, Zhengzhou 450001, PR China.
authors.forename: Duobin
authors.initials: D
authors.lastname: Mao
authors.affiliation: School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou 450001, PR China; Collaborative Innovation Center of Food Production and Safety, Henan Province, Zhengzhou 450001, PR China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Xu
keywords: Lentinus tigrinus; antiproliferative activity; exopolysaccharides; molecular conformation; molecular mass