title: TLR4 and Caveolin-1 in Monocytes Are Associated With Inflammatory Conditions in Diabetic Neuropathy.
abstract: The purpose of this study was to investigate the expression of TLR4 and caveolin-1 in monocytes among healthy volunteers as well as those with type-2 diabetes mellitus (T2DM) and diabetic peripheral neuropathy (DPN). Nineteen healthy control subjects, 18 patients with T2DM, and 20 patients with DPN were enrolled. Toll-like receptor (TLR)4, caveolin-1, MyD88, phosphorylated IκB, and plasma TNF-α and interleukin (IL)-6 were measured using real-time polymerase chain reaction, Western blotting, and enzyme-linked immunosorbent assay. Compared with the other two groups, the DPN group had higher expression of TLR4, MyD88, phosphorylated IκB, TNF-α, and IL-6, but significantly lower levels of caveolin-1 and total IκB in monocytes. Plasma concentrations of TNF-α and IL-6 were positively correlated with TLR4 and negatively correlated with caveolin-1 in patients with DPN. Plasma concentration of TLR4 was negatively correlated with caveolin-1 in patients with DPN. Reduced expression of caveolin-1 in monocytes could aggravate the TLR4-mediated inflammatory cascade.
authors.affiliation: Department of Anesthesiology, Songjiang Center Hospital, NanJing Medical University, Shanghai, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Anesthesiology, Songjiang Center Hospital, NanJing Medical University, Shanghai, China.|Department of Anesthesiology, Nanjing Hospital, Nanjing Medical University & Nanjing First Hospital, Nanjing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Meng
authors.affiliation: Department of Anesthesiology, Songjiang Center Hospital, NanJing Medical University, Shanghai, China.
authors.forename: J
authors.initials: J
authors.lastname: Ji
authors.affiliation: Department of Anesthesiology, Songjiang Center Hospital, NanJing Medical University, Shanghai, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Central Laboratory, Songjiang Hospital, First People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
authors.forename: X
authors.initials: X
authors.lastname: Lou
mesh_terms: D051242:Caveolin 1 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D003929:Diabetic Neuropathies / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D018836:Inflammation Mediators / Q000378:metabolism; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*; D016328:NF-kappa B / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D051197:Toll-Like Receptor 4 / Q000097:blood / Q000235:genetics / Q000378:metabolism*; D014409:Tumor Necrosis Factor-alpha / Q000097:blood
chemical_list: C495540:CAV1 protein, human; D051242:Caveolin 1; C508600:IL6 protein, human; D018836:Inflammation Mediators; D015850:Interleukin-6; D016328:NF-kappa B; D012333:RNA, Messenger; C495345:TLR4 protein, human; D051197:Toll-Like Receptor 4; D014409:Tumor Necrosis Factor-alpha