title: New Cross-Talk Layer between Ultraconserved Non-Coding RNAs, MicroRNAs and Polycomb Protein YY1 in Bladder Cancer.
abstract: MicroRNAs (miRNAs) are highly conserved elements in mammals, and exert key regulatory functions. Growing evidence shows that miRNAs can interact with another class of non-coding RNAs, so-called transcribed ultraconserved regions (T-UCRs), which take part in transcriptional, post-transcriptional and epigenetic regulation processes. We report here the interaction of miRNAs and T-UCRs as a network modulating the availability of these non-coding RNAs in bladder cancer cells. In our cell system, antagomiR-596 increased the expression of T-UCR 201+. Moreover, T-UCR 8+ silencing increased miR-596 expression, which in turn reduced total T-UCR 283+, showing that the perturbation of one element in this network changes the expression of other interactors. In addition, we identify the polycomb protein Yin Yang 1 (YY1) as mediator of binding between miR-596 and T-UCR 8+. These new findings describe for the first time a network between T-UCRs, miRNAs and YY1 protein, highlighting the existence of an additional layer of gene expression regulation.
authors.affiliation: Institute of Genetics and Biophysics-CNR. Via P. Castellino, 111, 80131 Naples, Italy. sara.terreri@igb.cnr.it.
authors.forename: Sara
authors.initials: S
authors.lastname: Terreri
authors.affiliation: Bioker srl multimedica spa, via Brin, 49/65 80142 Naples, Italy. dursomontano@gmail.com.
authors.forename: Montano
authors.initials: M
authors.lastname: Durso
authors.affiliation: Institute of Genetics and Biophysics-CNR. Via P. Castellino, 111, 80131 Naples, Italy. vincenza.colonna@igb.cnr.it.
authors.forename: Vincenza
authors.initials: V
authors.lastname: Colonna
authors.affiliation: Dipartimento di Farmacia, Università di Napoli "Federico II", 80131 Naples, Italy. alessandra.romanelli@unina.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Romanelli
authors.affiliation: Department of Translational Medical Sciences, University of Naples "Federico II", 80131 Naples, Italy. daniela.terracciano@unina.it.
authors.forename: Daniela
authors.initials: D
authors.lastname: Terracciano
authors.affiliation: Division of Urology, European Institute of Oncology, 20141 Milan, Italy. drmatteoferro@libero.it.
authors.forename: Matteo
authors.initials: M
authors.lastname: Ferro
authors.affiliation: Division of Urology, IRCS National Tumor Institute, 80131 Naples, Italy. sisto.perdona@istitutotumori.na.it.
authors.forename: Sisto
authors.initials: S
authors.lastname: Perdonà
authors.affiliation: Division of Urology, IRCS National Tumor Institute, 80131 Naples, Italy. luigi.castaldo@gmail.it.
authors.forename: Luigi
authors.initials: L
authors.lastname: Castaldo
authors.affiliation: Institute of Protein Biochemistry-CNR. Via P. Castellino, 111, 80131 Naples, Italy. f.febbraio@ibp.cnr.it.
authors.forename: Ferdinando
authors.initials: F
authors.lastname: Febbraio
authors.affiliation: Department of Biochemistry, Biophysic and General Pathology, University of Campania Luigi Vanvitelli, Via De Crecchio 7, 80138 Naples, Italy. filomena.denigris@unina2.it.
authors.forename: Filomena
authors.initials: F
authors.lastname: de Nigris
authors.affiliation: Institute of Genetics and Biophysics-CNR. Via P. Castellino, 111, 80131 Naples, Italy. amelia.cimmino@igb.cnr.it.
authors.forename: Amelia
authors.initials: A
authors.lastname: Cimmino
keywords: bladder cancer; interaction network; microRNAs; transcribed ultraconserved regions