title: Detection of HBV cccDNA Methylation from Clinical Samples by Bisulfite Sequencing and Methylation-Specific PCR.
abstract: Mapping of DNA methylation is essential in understanding the process of HBV covalently closed circular DNA (cccDNA) transcription. Here, bisulfite sequencing PCR and methylation-specific PCR, two PCR-based approaches used in determining and quantifying the DNA methylation pattern, are described.
authors.affiliation: Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, Shanghai, 200040, China.
authors.forename: Richeng
authors.initials: R
authors.lastname: Mao
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, IN, USA.
authors.forename: Haitao
authors.initials: H
authors.lastname: Guo
authors.affiliation: Department of Infectious Diseases, Huashan Hospital, Fudan University, 12 Middle Wulumuqi Road, Shanghai, 200040, China. jmzhang@fudan.edu.cn.
authors.forename: Jiming
authors.initials: J
authors.lastname: Zhang
mesh_terms: D018899:CpG Islands; D019175:DNA Methylation; D004270:DNA, Circular; D004279:DNA, Viral; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D017422:Sequence Analysis, DNA
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral
keywords: Bisulfite sequencing PCR; DNA methylation; HBV cccDNA; Methylation-specific PCR