title: Changes in Attenuated Total Reflection Fourier Transform Infrared Spectra as Blood Dries Out.
abstract: The time since deposition (TSD) of a bloodstain is a valuable piece of evidence for forensic scientists to determine the time at which a crime took place. The objective of this study was to determine whether attenuated total reflection (ATR) Fourier transform infrared (FTIR) spectroscopy could be used to estimate the TSD of a bloodstain in a relatively early period (from 0 min to the time required for the bloodstain to dry out). For this purpose, we used ATR-FTIR to study the variation in absorbance at certain wavelengths as rat and human blood sample dried out. The absorbance at 3308/cm (A3308) was found to have a close correlation with the TSD during this time period, and the changes in A3308 during the drying of rat and human blood drops under the same controlled conditions showed similar results. The current study indicates that ATR-FTIR spectroscopy has potential as a tool for estimating TSD at early time periods of blood deposition.
authors.affiliation: Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR, China.
authors.forename: Yinming
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR, China.
authors.forename: Bing
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR, China.
authors.forename: Chengzhi
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Yao
authors.affiliation: Department of Forensic Pathology, Institute of Forensic Science, Ministry of Justice, Shanghai, 200063, PR, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Huang
authors.affiliation: Department of Forensic Medicine, School of Medicine, Xi'an Jiaotong University, Xi'an, 710061, PR, China.
authors.forename: Zhenyuan
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000818:Animals; D001801:Blood Stains; D003890:Desiccation; D005554:Forensic Medicine; D006801:Humans; D016015:Logistic Models; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors
keywords: Fourier transform infrared spectroscopy; Logistic regression analysis; bloodstain; forensic pathology; forensic science; time since deposition