title: Common findings of blaCTX-M-55-encoding 104-139 kbp plasmids harbored by extended-spectrum β-lactamase-producing Escherichia coli in pork meat, wholesale market workers, and patients with urinary tract infection in Vietnam.
abstract: Extended-spectrum, β-lactamase-producing Escherichia coli (ESBL-E) harboring the blaCTX-M-55-encoding plasmid (ESBL-E55) has been reported to be associated with urinary tract infection (UTI). The aims of this study were to clarify the prevalence of ESBL-E55 in pork meats and workers from the same wholesale market, as well as patients with UTI from a nearby hospital in Vietnam; we also investigated the plasmids encoding blaCTX-M-55. Sequencing analysis showed that 66.6% of the ESBL-E isolated from pork meats contained blaCTX-M-55, whereas the gene was present in 25.0% of workers and 12.5% of patients with UTI. Plasmid analysis showed that several sizes of plasmid encoded blaCTX-M-55in ESBL-E55 isolated from pork meats, whereas ESBL-E55 isolated from workers and patients with UTI contained only 104-139 kbp of blaCTX-M-55-encoding plasmids. This indicates that the 104-139 kbp sizes of blaCTX-M-55-encoding plasmids were commonly disseminated in pork meats, wholesale market workers, and patients with UTI.
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T A V
authors.initials: TAV
authors.lastname: Hoang
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T N H
authors.initials: TNH
authors.lastname: Nguyen
authors.affiliation: School of Health Science, Faculty of Medicine, University of the Ryukyu, Okinawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Ueda
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: Q P
authors.initials: QP
authors.lastname: Le
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T T N
authors.initials: TTN
authors.lastname: Tran
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T N D
authors.initials: TND
authors.lastname: Nguyen
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T V K
authors.initials: TVK
authors.lastname: Dao
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: M T
authors.initials: MT
authors.lastname: Tran
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T T T
authors.initials: TTT
authors.lastname: Le
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T L
authors.initials: TL
authors.lastname: Le
authors.affiliation: Graduate School of Pharmaceutical Science, Osaka University, Yamadaoka 2-7, Suita, Osaka, 537-0025, Japan. t.nakayama-glocol@outlook.com.
authors.forename: T
authors.initials: T
authors.lastname: Nakayama
authors.affiliation: School of Health Science, Faculty of Medicine, University of the Ryukyu, Okinawa, Japan.
authors.forename: I
authors.initials: I
authors.lastname: Hirai
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: T H
authors.initials: TH
authors.lastname: Do
authors.affiliation: Department of Food Microbiology, Nha Trang Pasteur Institute, Nha Trang, Vietnam.
authors.forename: Q M
authors.initials: QM
authors.lastname: Vien
authors.affiliation: Graduate School of Pharmaceutical Science, Osaka University, Yamadaoka 2-7, Suita, Osaka, 537-0025, Japan.|Osaka Prefectural Institute of Public Health, Osaka, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Yamamoto
mesh_terms: D002353:Carrier State / Q000382:microbiology*; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000453:epidemiology / Q000382:microbiology*; D029968:Escherichia coli Proteins / Q000235:genetics*; D006801:Humans; D008460:Meat / Q000382:microbiology*; D017720:Molecular Epidemiology; D010957:Plasmids / Q000032:analysis*; D015995:Prevalence; D017422:Sequence Analysis, DNA; D014552:Urinary Tract Infections / Q000382:microbiology*; D014744:Vietnam / Q000453:epidemiology; D001618:beta-Lactamases / Q000235:genetics*
chemical_list: D029968:Escherichia coli Proteins; D001618:beta-Lactamases; C000602254:beta-lactamase CTX-M-55, E coli