title: Cellulose Biorefinery Based on a Combined Catalytic and Biotechnological Approach for Production of 5-HMF and Ethanol.
abstract: In this study, a combination of catalytic and biotechnological processes was proposed for the first time for application in a cellulose biorefinery for the production of 5-hydroxymethylfurfural (5-HMF) and bioethanol. Hydrolytic dehydration of the mechanically activated microcrystalline cellulose over a carbon-based mesoporous Sibunt-4 catalyst resulted in moderate yields of glucose and 5-HMF (21.1-25.1 and 6.6-9.4 %). 5-HMF was extracted from the resulting mixture with isobutanol and subjected to ethanol fermentation. A number of yeast strains were isolated that also revealed high thermotolerance (up to 50 °C) and resistance to inhibitors found in the hydrolysates. The strains Kluyveromyces marxianus C1 and Ogataea polymorpha CBS4732 were capable of producing ethanol from processed catalytic hydrolysates of cellulose at 42 °C, with yields of 72.0±5.7 and 75.2±4.3 % from the maximum theoretical yield of ethanol, respectively.
authors.affiliation: Boreskov Institute of Catalysis (BIC), 630090, Novosibirsk, Lavrentieva ave. 5, Russian Federation.|Novosibirsk State University (NSU), 630090, Novosibirsk, Pirogova str. 2, Russian Federation.
authors.forename: Ksenia N
authors.identifier: 0000-0002-7382-1470
authors.initials: KN
authors.lastname: Sorokina
authors.affiliation: Boreskov Institute of Catalysis (BIC), 630090, Novosibirsk, Lavrentieva ave. 5, Russian Federation.|Novosibirsk State Technical University (NSTU), 630037, Novosibirsk, Prosp. Karla Marksa, 20, Russian Federation.
authors.forename: Oxana P
authors.initials: OP
authors.lastname: Taran
authors.affiliation: Boreskov Institute of Catalysis (BIC), 630090, Novosibirsk, Lavrentieva ave. 5, Russian Federation.
authors.forename: Tatiana B
authors.initials: TB
authors.lastname: Medvedeva
authors.affiliation: Boreskov Institute of Catalysis (BIC), 630090, Novosibirsk, Lavrentieva ave. 5, Russian Federation.
authors.forename: Yuliya V
authors.initials: YV
authors.lastname: Samoylova
authors.affiliation: Boreskov Institute of Catalysis (BIC), 630090, Novosibirsk, Lavrentieva ave. 5, Russian Federation.
authors.forename: Alexandr V
authors.initials: AV
authors.lastname: Piligaev
authors.affiliation: Boreskov Institute of Catalysis (BIC), 630090, Novosibirsk, Lavrentieva ave. 5, Russian Federation.|Novosibirsk State University (NSU), 630090, Novosibirsk, Pirogova str. 2, Russian Federation.
authors.forename: Valentin N
authors.initials: VN
authors.lastname: Parmon
mesh_terms: D001709:Biotechnology / Q000379:methods*; D002384:Catalysis; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000378:metabolism*; D005662:Furaldehyde / Q000031:analogs & derivatives* / Q000302:isolation & purification / Q000378:metabolism; D005947:Glucose / Q000378:metabolism; D006868:Hydrolysis; D007716:Kluyveromyces / Q000378:metabolism; D055595:Mechanical Phenomena; D004718:Saccharomycetales / Q000378:metabolism; D012997:Solvents / Q000737:chemistry; D013696:Temperature
chemical_list: D012997:Solvents; D000431:Ethanol; C008046:5-hydroxymethylfurfural; D002482:Cellulose; D005662:Furaldehyde; D005947:Glucose; C109691:microcrystalline cellulose
keywords: 5-HMF; biomass conversion; biosynthesis; ethanol; heterogeneous catalysis