title: Protein-Restricted Diet Regulates Lipid and Energy Metabolism in Skeletal Muscle of Growing Pigs.
abstract: The aim of this work was to study the lipid metabolism and energy status of skeletal muscle of pigs as affected by dietary protein restriction. Eighteen growing pigs were distributed into three treatments, and each group was fed one of three levels (20, 17, and 14%) of crude protein (CP) diets. Our results showed that pigs fed the 20% CP had greater (P < 0.05) gain:feed and muscle weight than those fed the 14% CP, but no differences between the 20 and 17% CP treatments. Additionally, protein restriction tended to increase (P = 0.07) the content of intramuscular fat (IMF) and up-regulated (P < 0.05) expression of lipogenic-related genes. Energy status was changed and, concomitantly, AMP-activated protein kinase α pathway was inhibited by reducing the dietary protein level. These results indicate protein restriction could be useful to improve IMF content of pigs through regulating lipid metabolism and associated energy utilization in muscle.
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Changsha, Hunan 410125, China.|University of Chinese Academy of Sciences , Beijing 10008, China.
authors.forename: Yinghui
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Changsha, Hunan 410125, China.|Hunan Co-Innovation Center of Animal Production Safety, CICAPS, Hunan Collaborative Innovation Center for Utilization of Botanical Functional Ingredients , Changsha, Hunan 410128, China.
authors.forename: Fengna
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Changsha, Hunan 410125, China.|University of Chinese Academy of Sciences , Beijing 10008, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Changsha, Hunan 410125, China.|University of Chinese Academy of Sciences , Beijing 10008, China.
authors.forename: Yehui
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Changsha, Hunan 410125, China.|University of Chinese Academy of Sciences , Beijing 10008, China.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Laboratory of Animal Nutrition and Human Health, School of Biology, Hunan Normal University , Changsha, Hunan 410018, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Nutrition and Human Health, School of Biology, Hunan Normal University , Changsha, Hunan 410018, China.
authors.forename: Chaoyue
authors.initials: C
authors.lastname: Wen
authors.affiliation: Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production; Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture , Changsha, Hunan 410125, China.|Laboratory of Animal Nutrition and Human Health, School of Biology, Hunan Normal University , Changsha, Hunan 410018, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Yin
mesh_terms: D055372:AMP-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D000821:Animal Feed / Q000032:analysis; D000818:Animals; D018753:Diet, Protein-Restricted; D004044:Dietary Proteins / Q000378:metabolism*; D004734:Energy Metabolism; D050356:Lipid Metabolism; D018482:Muscle, Skeletal / Q000254:growth & development / Q000378:metabolism*; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha / Q000235:genetics / Q000378:metabolism; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism; D013552:Swine / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*
chemical_list: D004044:Dietary Proteins; D000071248:Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; D055372:AMP-Activated Protein Kinases; D056564:Sirtuin 1
keywords: energy status; fat; growing pigs; protein restriction; skeletal muscle