title: Identification of a cyclodextrin inclusion complex of antimicrobial peptide CM4 and its antimicrobial activity.
abstract: Antibacterial peptide CM4 (ABP-CM4) is a natural product isolated from the silkworm Bombyx mori. It is a small cationic peptide with broad-spectrum activities against harmful microorganisms and may be used as a novel food preservative. However, ABP-CM4 lacks tertiary structure in water-like solutions, which makes it more susceptible to proteases and labile when exposed to air. In this study, β-cyclodextrin (β-CD) was chosen to form an inclusion complex with ABP-CM4, which enhanced the physical and chemical properties of ABP-CM4 but did not decrease its antibacterial activity. The storage stability and susceptibility to proteinases of ABP-CM4 were apparently improved under the protection of β-CD. This technology could also be widely applied to other AMPs as an antimicrobial system to be used in the food industry.
authors.affiliation: Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang Province 311121, China; Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, Jiangsu Province 210046, China. Electronic address: lijianfeng@hznu.edu.cn.
authors.forename: Jian-Feng
authors.initials: JF
authors.lastname: Li
authors.affiliation: Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics & Biotechnology, Ministry of Education, Nanjing Forestry University, Nanjing, Jiangsu Province 210037, China.
authors.forename: Jia-Xin
authors.initials: JX
authors.lastname: Zhang
authors.affiliation: Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang Province 311121, China.
authors.forename: Zhi-Guo
authors.initials: ZG
authors.lastname: Wang
authors.affiliation: Nursing, Lishui University (Sino-forsign cooperation), Lishui, Zhejiang Province 323499, China.
authors.forename: Ying-Jia
authors.initials: YJ
authors.lastname: Yao
authors.affiliation: Guangdong University of Education, Guangzhou, Guangdong Province 510303, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Han
authors.affiliation: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, Jiangsu Province 210046, China.
authors.forename: You-Long
authors.initials: YL
authors.lastname: Zhao
authors.affiliation: Institute of Aging Research, School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang Province 311121, China.
authors.forename: Jun-Ping
authors.initials: JP
authors.lastname: Liu
authors.affiliation: Jiangsu Province Key Laboratory for Molecular and Medical Biotechnology, Life Sciences College, Nanjing Normal University, Nanjing, Jiangsu Province 210046, China.
authors.forename: Shuang-Quan
authors.initials: SQ
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D005638:Fruit / Q000187:drug effects* / Q000382:microbiology; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D047392:beta-Cyclodextrins
keywords: ABP-CM4; Antibacterial assay; Inclusion complex; Stability; β-Cyclodextrin