title: New Immunosuppressive Sphingoid Base and Ceramide Analogues in Wild Cordyceps.
abstract: A comprehensive identification of sphingoid bases and ceramides in wild Cordyceps was performed by integrating a sequential chromatographic enrichment procedure and an UHPLC-ultrahigh definition-Q-TOF-MS based sphingolipidomic approach. A total of 43 sphingoid bases and 303 ceramides were identified from wild Cordyceps, including 12 new sphingoid base analogues and 159 new ceramide analogues based on high-resolution MS and MS/MS data, isotope distribution, matching with the comprehensive personal sphingolipid database, confirmation by sphingolipid standards and chromatographic retention time rule. The immunosuppressive bioassay results demonstrated that Cordyceps sphingoid base fraction exhibits more potent immunosuppressive activity than ceramide fraction, elucidating the immunosuppressive ingredients of wild Cordyceps. This study represented the most comprehensive identification of sphingoid bases and ceramides from a natural source. The findings of this study provided an insight into therapeutic application of wild Cordyceps.
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
authors.forename: Jia-Ning
authors.initials: JN
authors.lastname: Mi
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Han
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, China.
authors.forename: Yingqiong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of TCM Evaluation and Translational Research, Department of Complex Prescription of TCM, China Pharmaceutical University, 639 Longmian Road, Nanjing 211198, China.
authors.forename: Junping
authors.initials: J
authors.lastname: Kou
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.
authors.forename: Jing-Rong
authors.initials: JR
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China.|International Institute for Translational Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou, China.
authors.forename: Zhi-Hong
authors.initials: ZH
authors.lastname: Jiang
mesh_terms: D000818:Animals; D002518:Ceramides / Q000737:chemistry / Q000627:therapeutic use*; D032661:Cordyceps / Q000737:chemistry*; D007166:Immunosuppressive Agents / Q000627:therapeutic use*; D008297:Male; D008813:Mice, Inbred ICR; D013110:Sphingosine / Q000737:chemistry / Q000627:therapeutic use; D053719:Tandem Mass Spectrometry
chemical_list: D002518:Ceramides; D007166:Immunosuppressive Agents; D013110:Sphingosine