title: Streptomyces effect on the bacterial microbiota associated to Crassostrea sikamea oyster.
abstract: OBJECTIVE
  To determine the composition and diversity of the microbiota associated to Crassostrea sikamea treated during 30 days with Streptomyces strains N7 and RL8.
  
  
  RESULTS
  DNA was extracted from oysters followed by 16S rRNA gene amplification and pyrosequencing. The highest and lowest species diversity richness was observed in the initial and final control group, whereas Streptomyces-treated oysters exhibited intermediate values. Proteobacteria was the most abundant phylum (81·4-95·1%), followed by Bacteroidetes, Actinobacteria and Firmicutes. The genera Anderseniella, Oceanicola, Roseovarius, Ruegeria, Sulfitobacter, Granulosicoccus and Marinicella encompassed the core microbiota of all experimental groups. The genus Bacteriovorax was detected in all groups except in the final control and the depurated N7, whereas Vibrio remained undetected in all Streptomyces-treated groups. RL8 was the only group that harboured the genus Streptomyces in its microbiota. Principal component analysis showed that Streptomyces strains significantly changed oyster microbiota with respect to the initial and final control.
  
  
  CONCLUSIONS
  Crassostrea sikamea treated with Streptomyces showed high species diversity and a microbiota composition shift, characterized by keeping the predator genus Bacteriovorax and decreasing the pathogenic Vibrio.
  
  
  CONCLUSIONS
  This is the first culture-independent study showing the effect of Streptomyces over the oyster microbiota. It also sheds light about the potential use of Streptomyces to improve mollusc health and safety for consumers after the depuration process.
authors.affiliation: Centro de Bioactivos Químicos, Universidad Central de Las Villas, Villa Clara, Cuba.
authors.forename: M
authors.initials: M
authors.lastname: García Bernal
authors.affiliation: Centro Universitario Regional del Este (CURE), Universidad de la República, Rocha, Uruguay.
authors.forename: N
authors.initials: N
authors.lastname: Trabal Fernández
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste S.C. (CIBNOR), La Paz, Baja California Sur, México.
authors.forename: P E
authors.initials: PE
authors.lastname: Saucedo Lastra
authors.affiliation: Centro de Bioactivos Químicos, Universidad Central de Las Villas, Villa Clara, Cuba.
authors.forename: R
authors.initials: R
authors.lastname: Medina Marrero
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste S.C. (CIBNOR), La Paz, Baja California Sur, México.
authors.forename: J M
authors.initials: JM
authors.lastname: Mazón-Suástegui
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D000818:Animals; D041963:Bacteroidetes / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D044822:Biodiversity; D049896:Crassostrea / Q000382:microbiology*; D020563:Gammaproteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D064307:Microbiota; D025341:Principal Component Analysis; D020560:Proteobacteria / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D041902:Rhodobacteraceae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D013302:Streptomyces / Q000235:genetics / Q000502:physiology*; D014733:Vibrio / Q000145:classification / Q000235:genetics / Q000302:isolation & purification
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Bacteriovorax; Streptomyces; Vibrio; microbiota; oyster; pyrosequencing