title: Evaluation of neovascularization patterns in an orthotopic rat glioma model with dynamic contrast-enhanced MRI.
abstract: Background Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) has been proved useful in evaluating glioma angiogenesis, but the utility in evaluating neovascularization patterns has not been reported. Purpose To evaluate in vivo real-time glioma neovascularization patterns by measuring glioma perfusion quantitatively using DCE-MRI. Material and Methods Thirty Sprague-Dawley rats were used to establish C6 orthotopic glioma model and underwent MRI and pathology detections. As MRI and pathology were performed at six time points (i.e. 4, 8, 12, 16, 20, and 24 days) post transplantation, neovascularization patterns were evaluated via DCE-MRI. Results Four neovascularization patterns were observed in glioma tissues. Sprout angiogenesis and intussusceptive microvascular growth located inside tumor, while vascular co-option and vascular mimicry were found in the tumor margin and necrotic area, respectively. Sprout angiogenesis and intussusceptive microvascular growth increased with Ktrans, Kep, and Vpinside tumor tissue. In addition, Kepand Vpwere positively correlated with sprout angiogenesis and intussusceptive microvascular growth. Vascular co-option was decreased at 12 and 16 days post transplantation and correlated negatively with Ktransand Kepdetected in the glioma margin, respectively. Changes of vascular mimicry showed no significant statistical difference at the six time points. Conclusion Our results indicate that DCE-MRI can evaluate neovascularization patterns in a glioma model. Furthermore, DCE-MRI could be an imaging biomarker for guidance of antiangiogenic treatments in humans in the future.
authors.affiliation: 1 Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Du
authors.initials: D
authors.lastname: Xuesong
authors.affiliation: 1 Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wei
authors.affiliation: 1 Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Heng
authors.affiliation: 1 Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Chen
authors.initials: C
authors.lastname: Xiao
authors.affiliation: 1 Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Wang
authors.initials: W
authors.lastname: Shunan
authors.affiliation: 1 Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Guo
authors.initials: G
authors.lastname: Yu
authors.affiliation: 1 Department of Radiology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.|2 State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing, PR China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Weiguo
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000000981:diagnostic imaging* / Q000473:pathology; D003287:Contrast Media; D004195:Disease Models, Animal; D019786:Gadolinium DTPA; D005910:Glioma / Q000000981:diagnostic imaging* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D008279:Magnetic Resonance Imaging / Q000379:methods*; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000473:pathology; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003287:Contrast Media; C064925:gadodiamide; D019786:Gadolinium DTPA
keywords: Glioma; antiangiogenic treatment; dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI); neovascularization; rat