title: The structure of the mite allergen Blo t 1 explains the limited antibody cross-reactivity to Der p 1.
abstract: The Blomia tropicalis (Blo t) mite species is considered a storage mite in temperate climate zones and an important source of indoor allergens causing allergic asthma and rhinitis in tropical and subtropical regions. Here, we report the crystal structure of one of the allergens from Blo t, recombinant proBlo t 1 (rproBlo t 1), determined at 2.1 Å resolution. Overall, the fold of rproBlo t 1 is characteristic for the pro-form of cysteine proteases from the C1A class. Structural comparison of experimentally mapped Der f 1/Der p1 IgG epitopes to the same surface patch on Blo t 1, as well as of sequence identity of surface-exposed residues, suggests limited cross-reactivity between these allergens and Blo t 1. This is in agreement with ELISA inhibition results showing that, although cross-reactive human IgE epitopes exist, there are unique IgE epitopes for both Blo t 1 and Der p 1.
authors.affiliation: Global Research, ALK-Abelló A/S, Hørsholm, Denmark.
authors.forename: K H
authors.initials: KH
authors.lastname: Meno
authors.affiliation: Biostructural Research, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: J S
authors.initials: JS
authors.lastname: Kastrup
authors.affiliation: Departments of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Khoo Teck Puat-National University Children's Medical Institute, The National University Health System, Singapore.
authors.forename: I-C
authors.initials: IC
authors.lastname: Kuo
authors.affiliation: Departments of Paediatrics, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.|Immunology Programme, Centre for Life Sciences, National University of Singapore, Singapore.
authors.forename: K Y
authors.initials: KY
authors.lastname: Chua
authors.affiliation: Biostructural Research, Department of Drug Design and Pharmacology, University of Copenhagen, Copenhagen, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Gajhede
mesh_terms: D000485:Allergens / Q000737:chemistry* / Q000276:immunology*; D000595:Amino Acid Sequence; D000818:Animals; D000918:Antibody Specificity / Q000276:immunology; D039741:Antigens, Dermatophagoides / Q000737:chemistry* / Q000276:immunology*; D060829:Arthropod Proteins / Q000737:chemistry* / Q000276:immunology*; D003429:Cross Reactions / Q000276:immunology*; D003546:Cysteine Endopeptidases / Q000737:chemistry* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology*; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry / Q000276:immunology; D011487:Protein Conformation; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology; D013329:Structure-Activity Relationship
chemical_list: D000485:Allergens; D039741:Antigens, Dermatophagoides; D060829:Arthropod Proteins; C473655:Blo t 1allergen, Blomia tropicalis; D010455:Peptides; D011994:Recombinant Proteins; D007073:Immunoglobulin E; D003546:Cysteine Endopeptidases; C451162:Dermatophagoides pteronyssinus antigen p 1
keywords: Blomia tropicalis mite allergen; crystal structure; cysteine protease; human IgE epitopes; pro-peptide