title: A Mouse Intra-Intestinal Infusion Model and its Application to the Study of Nanoparticle Distribution.
abstract: The oral route is the most preferable one when it comes to drug administration. Different animal models have been used to characterize the fate of potential medicines upon oral delivery but fail to clarify specific events occurring at localized sites of the gastrointestinal tract, particularly at the small intestine. We developed a new mouse intra-intestinal infusion model that enabled the direct administration of substances (such as drugs or nanoparticle drug carriers) in the small intestine through an implanted catheter, which can be maintained for prolonged periods of time. The location of catheter insertion can be previously determined as more proximal or distal, allowing to test specific portions of the intestine. Since the model is presumably able to maintain normal physiological characteristics, namely the mucus coating of the intestinal wall, it allowed studying the distribution of different nanoparticles upon localized intra-intestinal administration. The hereby proposed mouse model has the potential to be useful in other types of studies, namely in clarifying localized processes occurring at specific sites of the intestine.
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do PortoPorto, Portugal; Institute of Molecular Pathology and Immunology of the University of PortoPorto, Portugal; Faculty of Medicine of the University of PortoPorto, Portugal; Gastroenterology Department, Unidade Local Saúde da GuardaGuarda, Portugal; Gulbenkian Programme for Advanced Medical EducationLisbon, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Sadio
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do PortoPorto, Portugal; Institute of Molecular Pathology and Immunology of the University of PortoPorto, Portugal.
authors.forename: Ana L
authors.initials: AL
authors.lastname: Amaral
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do PortoPorto, Portugal; Instituto de Engenharia Biomédica, Universidade do PortoPorto, Portugal; Instituto de Ciências Biomédicas Abel Salazar, Universidade do PortoPorto, Portugal.
authors.forename: Rute
authors.initials: R
authors.lastname: Nunes
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do PortoPorto, Portugal; Institute of Molecular Pathology and Immunology of the University of PortoPorto, Portugal.
authors.forename: Sara
authors.initials: S
authors.lastname: Ricardo
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do PortoPorto, Portugal; Instituto de Engenharia Biomédica, Universidade do PortoPorto, Portugal; CESPU - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Instituto Universitário de Ciências da SaúdeGandra, Portugal.
authors.forename: Bruno
authors.initials: B
authors.lastname: Sarmento
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do PortoPorto, Portugal; Institute of Molecular Pathology and Immunology of the University of PortoPorto, Portugal; Faculty of Medicine of the University of PortoPorto, Portugal; Biology Department, Faculty of Sciences of the University of PortoPorto, Portugal.
authors.forename: Raquel
authors.initials: R
authors.lastname: Almeida
authors.affiliation: Department of Pathology, Southern California Research Center for ALPD and Cirrhosis, Keck School of Medicine of the University of Southern CaliforniaLos Angeles, CA, USA; Department of Veterans Affairs Greater Los Angeles Healthcare SystemLos Angeles, CA, USA.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tsukamoto
authors.affiliation: Instituto de Investigação e Inovação em Saúde, Universidade do PortoPorto, Portugal; Instituto de Engenharia Biomédica, Universidade do PortoPorto, Portugal.
authors.forename: José
authors.initials: J
authors.lastname: das Neves
keywords: mouse intra-intestinal model; mucoadhesive particles; mucus barrier; mucus penetrating particles; small bowel