title: Highly selective solid phase extraction and preconcentration of Azathioprine with nano-sized imprinted polymer based on multivariate optimization and its trace determination in biological and pharmaceutical samples.
abstract: In this research, for first time selective separation and determination of Azathioprine is demonstrated using molecularly imprinted polymer as the solid-phase extraction adsorbent, measured by spectrophotometry at λmax286nm. The selective molecularly imprinted polymer was produced using Azathioprine and methacrylic acid as a template molecule and monomer, respectively. A molecularly imprinted solid-phase extraction procedure was performed in column for the analyte from pharmaceutical and serum samples. The synthesized polymers were characterized by infrared spectroscopy (IR), field emission scanning electron microscopy (FESEM). In order to investigate the effect of independent variables on the extraction efficiency, the response surface methodology (RSM) based on Box-Behnken design (BBD) was employed. The analytical parameters such as precision, accuracy and linear working range were also determined in optimal experimental conditions and the proposed method was applied to analysis of Azathioprine. The linear dynamic range and limits of detection were 2.5-0.01 and 0.008mgL-1respectively. The recoveries for analyte were higher than 95% and relative standard deviation values were found to be in the range of 0.83-4.15%. This method was successfully applied for the determination of Azathioprine in biological and pharmaceutical samples.
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G. C., P.O. Box 19839-4716, Tehran, Iran. Electronic address: ss-hosseiny@cc.sbu.ac.ir.
authors.forename: Saied Saeed Hosseiny
authors.initials: SSH
authors.lastname: Davarani
authors.affiliation: Faculty of Chemistry, Shahid Beheshti University, G. C., P.O. Box 19839-4716, Tehran, Iran.
authors.forename: Zeinab
authors.initials: Z
authors.lastname: Rezayati Zad
authors.affiliation: Islamic Azad University, Ilam Branch, Ilam, Iran.
authors.forename: Ali Reza
authors.initials: AR
authors.lastname: Taheri
authors.affiliation: Islamic Azad University, Ilam Branch, Ilam, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Rahmatian
mesh_terms: D001379:Azathioprine / Q000032:analysis*; D054802:Molecular Imprinting; D053758:Nanoparticles / Q000737:chemistry*; D004364:Pharmaceutical Preparations / Q000032:analysis*; D011109:Polymethacrylic Acids / Q000737:chemistry*; D013055:Spectrophotometry, Infrared / Q000379:methods
chemical_list: D004364:Pharmaceutical Preparations; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D001379:Azathioprine
keywords: Azathioprine; Box–Behnken design; Nano molecularly imprinted polymer; Solid-phase extraction; Spectrophotometry