title: Isolation of detergent-resistant membranes (DRMs) from Escherichia coli.
abstract: Lipid rafts or membrane microdomains have been proposed to compartmentalize cellular processes by spatially organizing diverse molecules/proteins in eukaryotic cells. Such membrane microdomains were recently reported to also exist in a few bacterial species. In this work, we report the development of a procedure for membrane microdomain isolation from Escherichia coli plasma membranes as well as a method to purify the latter. The method here reported could easily be adapted to other gram-negative bacteria, wherein the isolation of this kind of sub-membrane preparation imposes special difficulties. The analysis of isolated membrane microdomains might provide important information on the nature and function of these bacterial structures and permit their comparison with the ones of eukaryotic cells.
authors.affiliation: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México City, Mexico.
authors.forename: José E
authors.initials: JE
authors.lastname: Guzmán-Flores
authors.affiliation: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México City, Mexico.
authors.forename: Adrián F
authors.initials: AF
authors.lastname: Alvarez
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México City, Mexico.
authors.forename: Sebastián
authors.initials: S
authors.lastname: Poggio
authors.affiliation: Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, México City, Mexico.
authors.forename: Marina
authors.initials: M
authors.lastname: Gavilanes-Ruiz
authors.affiliation: Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, México City, Mexico. Electronic address: dimitris@ifc.unam.mx.
authors.forename: Dimitris
authors.initials: D
authors.lastname: Georgellis
mesh_terms: D004926:Escherichia coli / Q000737:chemistry* / Q000378:metabolism; D021962:Membrane Microdomains / Q000737:chemistry* / Q000378:metabolism
keywords: Gram-negative bacteria; Membrane microdomains; SPFH-domain