title: Proteomic investigation of human cystic echinococcosis in the liver.
abstract: Cystic echinococcosis (CE) is a pandemic infectious disease caused by the tapeworm Echinococcus granulosus that forms cysts in different organs such as lungs and liver. Imaging examination and serological tests have some drawbacks such as low sensitivity. In this study, we used an up-to-date workflow of laser microdissection-based microproteomics and matrix-assisted laser desorption/ionization time-of-flight imaging mass spectrometry in order to depict the proteomic pattern of CE in the liver. This investigation revealed specific markers of a parasitic cyst in liver. This proteomic pattern could facilitate diagnosis of CE in the future.
authors.affiliation: Proteopath GmbH, Trier, Max Planck Strasse 17, 54296 Trier, Germany; Institute of Pathology, University of Heidelberg, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany. Electronic address: remi.longuespee@med.uni-heidelberg.de.
authors.forename: Rémi
authors.initials: R
authors.lastname: Longuespée
authors.affiliation: Proteopath GmbH, Trier, Max Planck Strasse 17, 54296 Trier, Germany.
authors.forename: Rita
authors.initials: R
authors.lastname: Casadonte
authors.affiliation: Institute of Pathology, University of Heidelberg, Im Neuenheimer Feld 224, 69120 Heidelberg, Germany.
authors.forename: Mark
authors.initials: M
authors.lastname: Kriegsmann
authors.affiliation: Molecular Pathology Trier, Max Planck Strasse 17, 54296 Trier, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Wandernoth
authors.affiliation: Department of Internal Medicine V, University of Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Lisenko
authors.affiliation: Mass Spectrometry Laboratory (LSM), Systems Biology and Chemical Biology, GIGA-Research, University of Liège, Allée du 6 aoÛt 11, 4000 Liège, Belgium.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Mazzucchelli
authors.affiliation: Bruker Daltonik GmbH, Bremen, Germany, Fahrenheitstrasse, 28359 Bremen, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Becker
authors.affiliation: Proteopath GmbH, Trier, Max Planck Strasse 17, 54296 Trier, Germany; Molecular Pathology Trier, Max Planck Strasse 17, 54296 Trier, Germany; MVZ for Histology, Cytology and Molecular Diagnostics Trier, Max Planck Strasse 5, 54296 Trier, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Kriegsmann
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D004444:Echinococcosis, Hepatic / Q000378:metabolism* / Q000469:parasitology* / Q000473:pathology; D048209:Echinococcus granulosus; D006801:Humans; D061346:Laser Capture Microdissection; D020543:Proteome; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry
chemical_list: D020543:Proteome
keywords: Cystic echinococcosis; Histoproteomics; Laser microdissection; MALDI IMS; Parasitic disease