title: Mechanism of Selective Nickel Transfer from HypB to HypA, Escherichia coli [NiFe]-Hydrogenase Accessory Proteins.
abstract: [NiFe]-hydrogenase enzymes catalyze the reversible reduction of protons to molecular hydrogen and serve as a vital component of the metabolism of many pathogens. The synthesis of the bimetallic catalytic center requires a suite of accessory proteins, and the penultimate step, nickel insertion, is facilitated by the metallochaperones HypA and HypB. In Escherichia coli, nickel moves from a site in the GTPase domain of HypB to HypA in a process accelerated by GDP. To determine how the transfer of nickel is controlled, the impacts of HypA and nucleotides on the properties of HypB were examined. Integral to this work was His2Gln HypA, a mutant with attenuated nickel affinity that does not support hydrogenase production in E. coli. This mutation inhibits the translocation of nickel from HypB. H2Q-HypA does not modulate the apparent metal affinity of HypB, but the stoichiometry and stability of the HypB-nickel complex are modulated by the nucleotide. Furthermore, the HypA-HypB interaction was detected by gel filtration chromatography if HypB was loaded with GDP, but not a GTP analogue, and the protein complex dissociated upon binding of nickel to His2 of HypA. In contrast, a nucleotide does not modulate the binding of zinc to HypB, and loading zinc into the GTPase domain of HypB inhibits formation of the complex with HypA. These results demonstrate that GTP hydrolysis controls both metal binding and protein-protein interactions, conferring selective and directional nickel transfer during [NiFe]-hydrogenase biosynthesis.
authors.affiliation: Department of Chemistry, University of Toronto , Toronto, Ontario, Canada M5S 3H6.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Lacasse
authors.affiliation: Department of Chemistry, University of Toronto , Toronto, Ontario, Canada M5S 3H6.
authors.forename: Colin D
authors.initials: CD
authors.lastname: Douglas
authors.affiliation: Department of Chemistry, University of Toronto , Toronto, Ontario, Canada M5S 3H6.|Department of Biochemistry, University of Toronto , Toronto, Ontario, Canada M5S 1A8.
authors.forename: Deborah B
authors.initials: DB
authors.lastname: Zamble
mesh_terms: D004926:Escherichia coli / Q000201:enzymology*; D006864:Hydrogenase / Q000378:metabolism*; D056511:Metallochaperones / Q000378:metabolism*; D009532:Nickel / Q000378:metabolism*; D013816:Thermodynamics
chemical_list: D056511:Metallochaperones; D009532:Nickel; C043296:nickel-iron hydrogenase; D006864:Hydrogenase