title: Tandem Mass Spectrometry in Combination with Product Ion Mobility for the Identification of Phospholipids.
abstract: Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. It was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 E. 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Karin A Zemski
authors.identifier: 0000-0002-7089-691X
authors.initials: KA
authors.lastname: Berry
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 E. 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Robert M
authors.identifier: 0000-0003-4711-6361
authors.initials: RM
authors.lastname: Barkley
authors.affiliation: National Renewable Energy Laboratory , 15013 Denver W Pkwy, Golden, Colorado 80401, United States.
authors.forename: Joseph J
authors.initials: JJ
authors.lastname: Berry
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 E. 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Joseph A
authors.initials: JA
authors.lastname: Hankin
authors.affiliation: Waters Corporation, Altrincham Road, Wilmslow SK9 4AX, United Kingdom.
authors.forename: Emmy
authors.initials: E
authors.lastname: Hoyes
authors.affiliation: Waters Corporation, Altrincham Road, Wilmslow SK9 4AX, United Kingdom.
authors.forename: Jeffery M
authors.initials: JM
authors.lastname: Brown
authors.affiliation: Department of Pharmacology, University of Colorado Denver , Mail Stop 8303, 12801 E. 17th Avenue, Aurora, Colorado 80045, United States.
authors.forename: Robert C
authors.identifier: 0000-0002-9430-6515
authors.initials: RC
authors.lastname: Murphy
mesh_terms: D006801:Humans; D013058:Mass Spectrometry; D015394:Molecular Structure; D010743:Phospholipids / Q000097:blood*; D053719:Tandem Mass Spectrometry
chemical_list: D010743:Phospholipids