title: A biotinylated piperazine-rhodol derivative: a 'turn-on' probe for nitroreductase triggered hypoxia imaging.
abstract: We developed a nitroreductase responsive theranostic probe 1; it comprises biotinylated rhodol in conjunction with p-nitrobenzyl functionality. The probe 1 showed a remarkable fluorescence 'turn-on' signal in the presence of nitroreductase under physiological conditions. The probe is considerably stable within a wide biological pH range (6-8) and also is very sensitive toward a reducing micro-environment e.g. liver microsome. Further, it enables providing cellular and in vivo nematode images in a reducing microenvironment.
authors.affiliation: College of Chemical Science and Technology, Yunnan University, 650091-Kunming, 650091, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Amrita Centre for Industrial Research & Innovation, Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, 641112, India. sankarprasad@cb.amrita.edu.
authors.forename: Kondapa Naidu
authors.initials: KN
authors.lastname: Bobba
authors.affiliation: College of Chemical Science and Technology, Yunnan University, 650091-Kunming, 650091, China.
authors.forename: Xue Wei
authors.initials: XW
authors.lastname: Lv
authors.affiliation: College of Chemical Science and Technology, Yunnan University, 650091-Kunming, 650091, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Yang
authors.affiliation: Amrita Centre for Industrial Research & Innovation, Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, 641112, India. sankarprasad@cb.amrita.edu.
authors.forename: Nithya
authors.initials: N
authors.lastname: Velusamy
authors.affiliation: College of Chemistry and Chemical Engineering, Yunnan University, Kunming, 650500, China. junfengzhang78@aliyun.com.
authors.forename: Jun Feng
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Amrita Centre for Industrial Research & Innovation, Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, 641112, India. sankarprasad@cb.amrita.edu and Department of Chemical Engineering & Materials Science, Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, 641112, India.
authors.forename: Sankarprasad
authors.identifier: 0000-0001-5080-0936
authors.initials: S
authors.lastname: Bhuniya
mesh_terms: D000818:Animals; D001710:Biotin / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000494:pharmacology* / Q000633:toxicity; D017173:Caenorhabditis elegans; D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity; D006801:Humans; D000860:Hypoxia / Q000000981:diagnostic imaging*; D007915:Lepidoptera; D057230:Limit of Detection; D008862:Microsomes, Liver / Q000378:metabolism; D009601:Nitroreductases / Q000378:metabolism*; D010879:Piperazines / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology / Q000633:toxicity; D051381:Rats; D000068936:Theranostic Nanomedicine; D044004:Xanthones / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology* / Q000633:toxicity
chemical_list: D005456:Fluorescent Dyes; D010879:Piperazines; D044004:Xanthones; C000607488:rhodol; D001710:Biotin; D009601:Nitroreductases