title: Polycaprolactone nanofibrous mesh reduces foreign body reaction and induces adipose flap expansion in tissue engineering chamber.
abstract: Tissue engineering chamber technique can be used to generate engineered adipose tissue, showing the potential for the reconstruction of soft tissue defects. However, the consequent foreign body reaction induced by the exogenous chamber implantation causes thick capsule formation on the surface of the adipose flap following capsule contracture, which may limit the internal tissue expansion. The nanotopographical property and architecture of nanofibrous scaffold may serve as a promising method for minimizing the foreign body reaction. Accordingly, electrospinning porous polycaprolactone (PCL) nanofibrous mesh, a biocompatible synthetic polymer, was attached to the internal surface of the chamber for the reducing local foreign body reaction. Adipose flap volume, level of inflammation, collagen quantification, capsule thickness, and adipose tissue-specific gene expression in chamber after implantation were evaluated at different time points. The in vivo study revealed that the engineered adipose flaps in the PCL group had a structure similar to that in the controls and normal adipose tissue structure but with a larger flap volume. Interleukin (IL)-1β, IL-6, and transforming growth factor-β expression decreased significantly in the PCL group compared with the control. Moreover, the control group had much more collagen deposition and thicker capsule than that observed in the PCL group. These results indicate that the unique nanotopographical effect of electrospinning PCL nanofiber can reduce foreign body reaction in a tissue engineering chamber, which maybe a promising new method for generating a larger volume of mature, vascularized, and stable adipose tissue.
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Luo
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China; Department of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Yunfan
authors.initials: Y
authors.lastname: He
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China; Department of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Chang
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Gan
authors.initials: G
authors.lastname: Xie
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Weiqing
authors.initials: W
authors.lastname: Zhan
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Xuecen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: Department of Mechanical Engineering, Biochemistry and Medical Genetics, University of Manitoba, Winnipeg, Manitoba, Canada; Children's Hospital Research Institute of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Malcolm
authors.initials: M
authors.lastname: Xing
authors.affiliation: Department of Plastic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Lu
mesh_terms: D000273:Adipose Tissue / Q000187:drug effects*; D000818:Animals; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry; D005549:Foreign-Body Reaction / Q000276:immunology*; D053583:Interleukin-1beta / Q000378:metabolism; D015850:Interleukin-6 / Q000378:metabolism; D008297:Male; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D009765:Obesity; D011091:Polyesters / Q000737:chemistry*; D016062:Porosity; D051381:Rats; D017207:Rats, Sprague-Dawley; D012038:Regeneration; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry; D016212:Transforming Growth Factor beta / Q000378:metabolism
chemical_list: D053583:Interleukin-1beta; D015850:Interleukin-6; D011091:Polyesters; D016212:Transforming Growth Factor beta; C016240:polycaprolactone; D003094:Collagen
keywords: adipose tissue regeneration; foreign body reaction; polycaprolactone nanofibrous mesh; porous structure; topography