title: Serum MicroRNA Profiling and Bioinformatics of Patients with Spleen-Deficiency Syndrome.
abstract: To investigate serum microRNA (miRNA) profile and bioinformatics of patients with spleen-deficiency syndrome (SDS) and explore pathogenesis of SDS patients from miRNA levels, 10 patients with type 2 diabetes mellitus (T2DM), within which 5 patients were with SDS and the remaining were with blood stasis syndrome (BSS), and 5 healthy volunteers were recruited. Serum miRNA profiles of SDS patients were identified by quantitative PCR array. Target prediction and functional annotation for miRNAs were performed by miRSystem database. The present study identified 11 candidate serum miRNAs for SDS patients, and their targets were significantly enriched in 18 KEGG pathways and 7 GO molecular functions. Those enriched KEGG pathways included (1) metabolisms of carbohydrate, protein, amino acid, and fatty acid, (2) signaling pathways of insulin, ErbB, chemokine, calcium, and type II diabetes mellitus, (3) invasions of bacterium,Escherichia coli, andShigella(Shigellosis), and (4) endocytosis and phagocytosis. Those enriched GO molecular functions were mainly involved in transcription regulation and regulation of metabolism. Our findings might elucidate the pathogenesis of SDS patients with disorders of substance metabolism and hypoimmunity from miRNA levels, as well as providing some miRNA biomarkers for clinical syndrome differentiation of SDS.
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, No. 280 Waihuan Road East, Higher Education Mega Center, Panyu District, Guangzhou 510006, China.
authors.forename: Ze-Min
authors.identifier: 0000-0002-3053-2126
authors.initials: ZM
authors.lastname: Yang
authors.affiliation: Pi-Wei Institute, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Baiyun District, Guangzhou 510405, China.
authors.forename: Long-Hui
authors.initials: LH
authors.lastname: Chen
authors.affiliation: Department of Traditional Chinese Medicine, First Affiliated Hospital of Guangdong Pharmaceutical University, No. 19 Nonglinxia Road, Guangzhou 510080, China.
authors.forename: Min
authors.initials: M
authors.lastname: Hong
authors.affiliation: Department of Traditional Chinese Medicine, First Affiliated Hospital of Guangdong Pharmaceutical University, No. 19 Nonglinxia Road, Guangzhou 510080, China.
authors.forename: Ying-Yu
authors.identifier: 0000-0002-1415-337X
authors.initials: YY
authors.lastname: Chen
authors.affiliation: Clinical Laboratory, First Affiliated Hospital of Guangdong Pharmaceutical University, No. 19 Nonglinxia Road, Guangzhou 510080, China.
authors.forename: Xiao-Rong
authors.initials: XR
authors.lastname: Yang
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, No. 280 Waihuan Road East, Higher Education Mega Center, Panyu District, Guangzhou 510006, China.
authors.forename: Si-Meng
authors.initials: SM
authors.lastname: Tang
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, No. 280 Waihuan Road East, Higher Education Mega Center, Panyu District, Guangzhou 510006, China.
authors.forename: Qian-Fa
authors.initials: QF
authors.lastname: Yuan
authors.affiliation: School of Basic Courses, Guangdong Pharmaceutical University, No. 280 Waihuan Road East, Higher Education Mega Center, Panyu District, Guangzhou 510006, China.
authors.forename: Zhen-Yu
authors.initials: ZY
authors.lastname: He
authors.affiliation: Pi-Wei Institute, Guangzhou University of Chinese Medicine, No. 12 Jichang Road, Baiyun District, Guangzhou 510405, China.
authors.forename: Wei-Wen
authors.initials: WW
authors.lastname: Chen