title: Structural characterization and antioxidant activity of a glucan from Meripilus giganteus.
abstract: A new water soluble glucan (MGPS), with a molecular weight ∼1.48×105Da, was isolated from the fruit bodies of Meripilus giganteus by hot water extraction followed by purification through dialysis tubing cellulose membrane and sepharose 6B column chromatography. Its structural characteristics were investigated by acid hydrolysis, methylation analysis, Smith degradation and 1D/2D NMR experiments. The monosaccharide composition analysis showed that MGPS contain only glucose. The backbone of MGPS was composed of two (1→3)-β-d-glucopyranosyl, two (1→6)-β-d-glucopyranosyl, two (1→6)-α-d-glucopyranosyl, and one (1→4)-α-d-glucopyranosyl residues, out of which one (1→3)-β-d-glucopyranosyl residue was branched at O-6 position with terminal α-d-glucopyranosyl residue and one (1→4)-α-d-glucopyranosyl residue branched at O-6 position with terminal β-d-glucopyranosyl residue. In vitro antioxidant studies showed that the MGPS exhibited hydroxide radical scavenging activity (IC50=390μg/mL), superoxide radical scavenging activity (IC50=70μg/mL), and ferrous ion chelating activity (IC50=290μg/mL).
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Manna
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Manabendra
authors.initials: M
authors.lastname: Pattanayak
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Ipsita K
authors.initials: IK
authors.lastname: Sen
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Sunil K
authors.initials: SK
authors.lastname: Bhanja
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Surajit
authors.initials: S
authors.lastname: Samanta
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India.
authors.forename: Bibhash C
authors.initials: BC
authors.lastname: Panda
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Soumitra
authors.initials: S
authors.lastname: Paloi
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D002240:Carbohydrate Sequence; D002614:Chelating Agents / Q000737:chemistry; D048690:Fruiting Bodies, Fungal / Q000737:chemistry; D005936:Glucans / Q000737:chemistry*; D006868:Hydrolysis; D009682:Magnetic Resonance Spectroscopy
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D005936:Glucans
keywords: Antioxidant activity; Edible mushrooms; Glucan; Meripilus giganteus (Pers.) P. karst; NMR studies