title: Induction of protective immunity against influenza A/Jiangxi-Donghu/346/2013 (H10N8) in mice.
abstract: Human infections with A/Jiangxi-Donghu/346/2013 (H10N8) virus have raised concerns about its pandemic potential. In order to develop a vaccine against this virus, the immunogenicity of its haemagglutinin protein was evaluated in mice. Using both whole-virion and recombinant subunit protein vaccines, we showed that two doses of either vaccine elicited neutralizing antibody responses. The protective efficacy of the vaccine-induced responses was assessed using a reverse-genetics-derived H10 reassortant virus on the A/Puerto Rico/8/34 (H1N1) backbone. The reassortant virus replicated efficiently in the respiratory tract of unvaccinated mice whereas vaccinated mice were completely protected from challenge, with no detectable viral load in the lower respiratory tract. Finally, the serum neutralizing antibody responses elicited by the H10 vaccines also exhibited cross-neutralizing activity against three heterologous wild-type H10 viruses. Collectively, these findings demonstrate that different vaccine platforms presenting the H10 haemagglutinin protein induce protective immunity.
authors.affiliation: Host-Pathogen Interactions Laboratory, DMERI, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.
authors.forename: Li-Fang
authors.initials: LF
authors.lastname: Kuah
authors.affiliation: Host-Pathogen Interactions Laboratory, DMERI, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.
authors.forename: Lay-Hoon
authors.initials: LH
authors.lastname: Tang
authors.affiliation: Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
authors.forename: Troy
authors.initials: T
authors.lastname: Sutton
authors.affiliation: Host-Pathogen Interactions Laboratory, DMERI, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.
authors.forename: Jie-Hui
authors.initials: JH
authors.lastname: Lim
authors.affiliation: Program in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore.
authors.forename: Wan-Ling
authors.initials: WL
authors.lastname: Sin
authors.affiliation: Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
authors.forename: Elaine
authors.initials: E
authors.lastname: Lamirande
authors.affiliation: Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, MD 20892, USA.
authors.forename: Kanta
authors.initials: K
authors.lastname: Subbarao
authors.affiliation: Host-Pathogen Interactions Laboratory, DMERI, DSO National Laboratories, 27 Medical Drive, Singapore 117510, Singapore.|Program in Emerging Infectious Diseases, Duke-NUS Medical School, 8 College Road, Singapore 169857, Singapore.
authors.forename: Yuk-Fai
authors.initials: YF
authors.lastname: Lau
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood*; D000914:Antibodies, Viral / Q000097:blood*; D003429:Cross Reactions; D004285:Dogs; D057809:HEK293 Cells; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics / Q000276:immunology*; D006801:Humans; D000071497:Immunogenicity, Vaccine; D065926:Influenza A Virus, H10N8 Subtype / Q000235:genetics / Q000276:immunology* / Q000502:physiology; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000276:immunology; D007252:Influenza Vaccines / Q000235:genetics / Q000276:immunology*; D007251:Influenza, Human / Q000097:blood / Q000276:immunology / Q000517:prevention & control; D061985:Madin Darby Canine Kidney Cells; D051379:Mice; D008807:Mice, Inbred BALB C; D009976:Orthomyxoviridae Infections / Q000097:blood* / Q000276:immunology / Q000517:prevention & control; D016865:Reassortant Viruses / Q000235:genetics / Q000276:immunology; D012137:Respiratory System / Q000821:virology; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology; D014779:Virus Replication
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D019267:Hemagglutinin Glycoproteins, Influenza Virus; D007252:Influenza Vaccines; D014614:Vaccines, Synthetic