title: Comprehensive Mass Cytometry Analysis of Cell Cycle, Activation, and Coinhibitory Receptors Expression in CD4 T Cells from Healthy and HIV-Infected Individuals.
abstract: Mass cytometry allows large multiplex analysis of cell cycle stages together with differentiation, activation, and exhaustion markers, allowing further assessment of the quiescence status of resting CD4 T cells.
  
  
  
  Peripheral blood CD4 T lymphocytes from 8 individuals, 4 healthy donors, and 4 HIV-infected on antiretroviral treatment (T) were stained with the same 26 monoclonal antibodies and dyes targeting surface and intracellular markers of differentiation, activation, exhaustion, and cell cycle stages. Samples were run on a CYTOF-2.
  
  
  
  Patterns of naïve [TN] CD4 T cells strongly differed from all other memory subsets central-memory (CM), transitional-memory (TM), effector-memory (EM), and terminally differentiated RA-expressing (TEMRA) subsets, while stem-cell memory (SCM) and T follicular-helper cells (TfH) were close to CM and TM cells with the highest percentages in cell cycle. EM and TEMRA were the most altered by HIV infection, with an increased frequency of activated and cycling cells. Activation markers and coinhibitory receptor expression differed among cell cycle stages, with HLA-DR fitting better than CD25 or CD38 with cycle, and opposite PD-1 gradients along differentiation and cell cycle. "Resting" DR-CD25- CD4+ T cells contained similar amounts of cells in G1 than the activated DR ± CD25± ones but three fold lower cells in S-G2-M.
  
  
  
  This broad multiplex mass cytometry analysis demonstrates some subsets of the so-called "resting" CD25-DR- CD4+ T cells contain noticeable amounts of cells into cycle or expressing coinhibitory receptors, opening new avenues for a redefinition of resting peripheral blood CD4 T cells harboring the HIV reservoirs. © 2016 International Clinical Cytometry Society.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM, U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris, UMRS 1135), Paris, F-75013, France.|UPMC Univ Paris 06, Plateforme de Cytométrie (CyPS), UMS30-LUMIC, Faculté de Médecine Pierre et Marie Curie, Site Pitié-Salpêtrière, Paris, F-75013, France.|EPHE, PSL Research University, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, Université Grenoble Alpes, Grenoble, France.
authors.forename: Aurélien
authors.initials: A
authors.lastname: Corneau
authors.affiliation: Commissariat de l'Energie Atomique (CEA) INSERM, Paris Sud University, UMR 1184, IDMIT Infrastructures, Fontenay aux Roses, F-92265, France.
authors.forename: Antonio
authors.initials: A
authors.lastname: Cosma
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM, U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris, UMRS 1135), Paris, F-75013, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Even
authors.affiliation: AP-HP, Hôpital Pitié-Salpêtrière, Service de Maladies Infectieuses et Tropicales, Paris, F-75013, France.|Sorbonne Universités, UPMC Univ Paris 06, INSERM, U1136, Institut Pierre Louis d'Epidémiologie et de Santé Publique (IPLESP UMRS 1136), Paris, F-75013, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Katlama
authors.affiliation: Commissariat de l'Energie Atomique (CEA) INSERM, Paris Sud University, UMR 1184, IDMIT Infrastructures, Fontenay aux Roses, F-92265, France.
authors.forename: Roger
authors.initials: R
authors.lastname: Le Grand
authors.affiliation: EPHE, PSL Research University, Institute for Advanced Biosciences, INSERM U1209, CNRS UMR5309, Université Grenoble Alpes, Grenoble, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Frachet
authors.affiliation: UPMC Univ Paris 06, Plateforme de Cytométrie (CyPS), UMS30-LUMIC, Faculté de Médecine Pierre et Marie Curie, Site Pitié-Salpêtrière, Paris, F-75013, France.
authors.forename: Catherine
authors.initials: C
authors.lastname: Blanc
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, INSERM, U1135, Centre d'Immunologie et des Maladies Infectieuses (CIMI-Paris, UMRS 1135), Paris, F-75013, France.|UPMC Univ Paris 06, Plateforme de Cytométrie (CyPS), UMS30-LUMIC, Faculté de Médecine Pierre et Marie Curie, Site Pitié-Salpêtrière, Paris, F-75013, France.|Département d'Immunologie, AP-HP, Hôpital Pitié-Salpêtrière, Paris, F-75013, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Autran
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000166:cytology* / Q000276:immunology / Q000821:virology; D002453:Cell Cycle / Q000502:physiology; D005434:Flow Cytometry / Q000379:methods; D015658:HIV Infections / Q000175:diagnosis / Q000276:immunology*; D006801:Humans; D008213:Lymphocyte Activation / Q000276:immunology*; D016176:T-Lymphocyte Subsets / Q000166:cytology* / Q000276:immunology / Q000821:virology
keywords: CD4 T cells; HIV; activation; cell cycle; coinhibitory receptors; mass cytometry; resting cells