title: Impaired ApoB-Lipoprotein and Triglyceride Metabolism in Obese Adolescents With Polycystic Ovary Syndrome.
abstract: BACKGROUND
  Adolescents with polycystic ovary syndrome (PCOS) have atherogenic dyslipidemia and increased cardiovascular disease (CVD) risk, and this is exacerbated in obesity.
  
  
  OBJECTIVE
  To determine and compare fasting and nonfasting lipid and apolipoprotein (Apo)B-lipoprotein metabolism in 3 groups of adolescent girls: healthy-weight controls, obese without PCOS (obese-control), and obese with PCOS (obese-PCOS).
  
  
  METHODS
  Participants aged 12 to 17 years were recruited for this cross-sectional study from a pediatric weight management clinic and the local community in Alberta, Canada.
  
  
  METHODS
  Plasma lipids and ApoB lipoproteins, including triglycerides (TGs) and ApoB100- and ApoB48-lipoproteins, were measured in the fasted and postprandial state following a high-fat meal.
  
  
  RESULTS
  Obese-control (n = 12) and obese-PCOS (n = 18) groups had twofold higher concentrations of fasting plasma TG and ApoB100- and ApoB48-lipoprotein remnants compared to healthy-weight controls (n = 10) (ApoB48-lipoproteins: 19.32 ± 2.10, 24.02 ± 4.28, and 8.95 ± 1.05 μg/mL, respectively; P < 0.001). The obese-PCOS group had 50% higher fasting plasma TG level compared to the obese-control group. The postprandial response was higher in both obese-controls and obese-PCOS subjects compared with healthy-weight controls in plasma TG area under the curve (AUC) (1028.0 ± 83.67, 1587.01 ± 259.6, and 615.42 ± 76.42 μg/mL⋅h, respectively; P < 0.01) and ApoB48(AUC) (191.30 ± 19.06, 238.8 ± 37.73, and 96.58 ± 9.17 μg/mL⋅h, respectively; P < 0.0001). Nonfasting plasma TG(AUC) and ApoB48(AUC) were positively correlated with free testosterone (r = 0.38; P < 0.001 and r = 0.33; P < 0.05, respectively), and these relationships were highly associated with insulin and body mass index.
  
  
  CONCLUSIONS
  Adolescent girls with obesity and PCOS have elevated fasting and postprandial plasma TG and ApoB-lipoprotein remnants, providing evidence of early subclinical CVD risk, and these indices are highly associated with impaired insulin metabolism and hyperandrogenemia.
authors.affiliation: Metabolic and Cardiovascular Diseases Laboratory, Alberta Diabetes Institute, and.
authors.forename: Donna F
authors.initials: DF
authors.lastname: Vine
authors.affiliation: Metabolic and Cardiovascular Diseases Laboratory, Alberta Diabetes Institute, and.
authors.forename: Ye
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Pediatrics, University of Alberta, Edmonton, Alberta T6G 2R3, Canada.
authors.forename: Mary M
authors.initials: MM
authors.lastname: Jetha
authors.affiliation: Department of Pediatrics, University of Alberta, Edmonton, Alberta T6G 2R3, Canada.
authors.forename: Geoff D
authors.initials: GD
authors.lastname: Ball
authors.affiliation: Metabolic and Cardiovascular Diseases Laboratory, Alberta Diabetes Institute, and.
authors.forename: Spencer D
authors.initials: SD
authors.lastname: Proctor
mesh_terms: D000293:Adolescent; D053299:Apolipoprotein B-100 / Q000378:metabolism*; D053283:Apolipoprotein B-48 / Q000378:metabolism*; D001055:Apolipoproteins B / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D015992:Body Mass Index; D016022:Case-Control Studies; D002648:Child; D003430:Cross-Sectional Studies; D005215:Fasting / Q000378:metabolism; D005260:Female; D006801:Humans; D007328:Insulin / Q000378:metabolism; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D011085:Polycystic Ovary Syndrome / Q000150:complications / Q000378:metabolism*; D019518:Postprandial Period; D013739:Testosterone / Q000378:metabolism; D014280:Triglycerides / Q000378:metabolism*
chemical_list: D053299:Apolipoprotein B-100; D053283:Apolipoprotein B-48; D001055:Apolipoproteins B; D001786:Blood Glucose; D007328:Insulin; D014280:Triglycerides; D013739:Testosterone