title: Selective serotonin reuptake inhibitor sertraline inhibits voltage-dependent K+ channels in rabbit coronary arterial smooth muscle cells.
abstract: We examined the effects of the selective serotonin reuptake inhibitor (SSRI) sertraline on voltage-dependent K+ (Kv) channels in freshly isolated rabbit coronary arterial smooth muscle cells using the voltage-clamp technique. Sertraline decreased the Kv channel current in a dose-dependent manner, with an IC50 value of 0.18 mu M and a slope value (Hill coefficient) of 0.61. Although the application of 1 mu M sertraline did not affect the steady-state activation curves, sertraline caused a significant, negative shift in the inactivation curves. Pretreatment with another SSRI, paroxetine, had no significant effect on Kv currents and did not alter the inhibitory effects of sertraline on Kv currents. From these results, we concluded that sertraline dose-dependently inhibited Kv currents independently of serotonin reuptake inhibition by shifting inactivation curves to a more negative potential.
authors.affiliation: Department of Physiology, Kangwon National University School of Medicine Chuncheon 200-701, South Korea.
authors.forename: Han Sol
authors.initials: HS
authors.lastname: Kim
authors.forename: Hongliang
authors.initials: H
authors.lastname: Li
authors.forename: Hye Won
authors.initials: HW
authors.lastname: Kim
authors.forename: Sung Eun
authors.initials: SE
authors.lastname: Shin
authors.forename: Il-Whan
authors.initials: IW
authors.lastname: Choi
authors.forename: Amy L
authors.initials: AL
authors.lastname: Firth
authors.forename: Hyoweon
authors.initials: H
authors.lastname: Bang
authors.forename: Young Min
authors.initials: YM
authors.lastname: Bae
authors.forename: Won Sun
authors.initials: WS
authors.lastname: Park
mesh_terms: D000818:Animals; D003331:Coronary Vessels / Q000187:drug effects / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D032389:Myocytes, Smooth Muscle / Q000187:drug effects / Q000473:pathology; D017374:Paroxetine / Q000008:administration & dosage; D018408:Patch-Clamp Techniques; D024642:Potassium Channels, Voltage-Gated / Q000096:biosynthesis* / Q000378:metabolism; D011817:Rabbits; D012701:Serotonin / Q000378:metabolism*; D017367:Selective Serotonin Reuptake Inhibitors / Q000008:administration & dosage*; D020280:Sertraline / Q000008:administration & dosage*
chemical_list: D017374:Paroxetine; D024642:Potassium Channels, Voltage-Gated; D012701:Serotonin; D017367:Selective Serotonin Reuptake Inhibitors; D020280:Sertraline