title: Antibacterial Activity of Two Chemomechanical Caries Removal Gels on Carious Dentin of Primary Teeth: An in vitro Study.
abstract: BACKGROUND
  Chemomechanical caries removal (CMCR) is an effective method of caries removal especially for primary teeth as they cause less discomfort when compared with conventional caries removal. The most significant thing about caries removal is the elimination of cariogenic bacteria. This study compares the antibacterial activity of two CMCR gels.
  
  
  METHODS
  A total of 40 primary molar teeth with carious dentin were split along the long axis in a laboratory. Total viable count (TVC) was taken for the teeth before splitting as a measure of colony-forming units per milliliter (CFU/mL). Each half was treated with either Carisolv or Carie-Care CMCR gels. Clean dentin samples were evaluated for Streptococcus mutans (SM) and Lactobacillus acidophilus (LB) after removal of carious tissue using the caries removal gels using serial dilutions and incubating on specific agar plates.
  
  
  RESULTS
  The results showed significant reduction in mean TVC after use of both the CMCR gels. Both gels reduced the CFU/mL of SM and LB to a significant level (p < 0.05). However, there was no significant difference between the antibacterial activities of the two CMCR gels.
  
  
  CONCLUSIONS
  The CMCR gels (Carisolv and Carie-Care) significantly reduced the residual TVC as well as SM and LB in carious primary dentin. Both CMCR gels had a similar antibacterial activity on the carious dentin of primary teeth.
  
  
  CONCLUSIONS
  The CMCR gels tested have a significant antibacterial activity and can be effectively used for elimination of caries-causing bacteria in primary teeth.
authors.affiliation: Pediatric Dentistry Division, Department of Preventive Dental Sciences, College of Dentistry, Dar Al Uloom University, Riyadh Kingdom of Saudi Arabia, Phone: +00966114949160, e-mail: shabnum@dau.edu.sa.
authors.forename: Shabnam
authors.initials: S
authors.lastname: Gulzar
authors.affiliation: Department of Pedodontics and Preventive Dentistry, Darshan Dental College and Hospital, Udaipur, Rajasthan, India.
authors.forename: Ruchi
authors.initials: R
authors.lastname: Arora
authors.affiliation: Department of Preventive Dental Sciences, College of Dentistry Dar Al Uloom University, Riyadh, Kingdom of Saudi Arabia Fellow Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India.
authors.forename: Altaf H
authors.initials: AH
authors.lastname: Shah
authors.affiliation: Department of Microbiology, Regional Research Centre, Udaipur Rajasthan, India.
authors.forename: Bhupendra
authors.initials: B
authors.lastname: Bhardwaj
authors.affiliation: Department of Microbiology, College of Science, King Saud University; Department of Medical Laboratory Science, College of Applied Medical Sciences, Prince Sattam bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia.
authors.forename: Ghadah
authors.initials: G
authors.lastname: Abusalim
authors.affiliation: Department of Oral and Maxillofacial Surgery, College of Dentistry, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Hesham S
authors.initials: HS
authors.lastname: Khalil
authors.affiliation: Department of Pediatric Dentistry and Head of Postgraduate Programe, College of Dentistry, King Saud University, Riyadh Kingdom of Saudi Arabia.
authors.forename: Amjad H
authors.initials: AH
authors.lastname: Wyne
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D029441:Carica; D003731:Dental Caries / Q000382:microbiology / Q000628:therapy*; D003737:Dental Cavity Preparation / Q000379:methods*; D003804:Dentin / Q000382:microbiology; D005782:Gels; D018698:Glutamic Acid / Q000494:pharmacology*; D006801:Humans; D066298:In Vitro Techniques; D007779:Lactobacillus acidophilus / Q000187:drug effects* / Q000302:isolation & purification; D007930:Leucine / Q000494:pharmacology*; D008239:Lysine / Q000494:pharmacology*; D008963:Molar; D010936:Plant Extracts / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000302:isolation & purification; D014094:Tooth, Deciduous
chemical_list: D000900:Anti-Bacterial Agents; C119934:Carisolv; D005782:Gels; D010936:Plant Extracts; D018698:Glutamic Acid; D007930:Leucine; D008239:Lysine
keywords: Antibacterial activity; Carie-Care; Carisolv; Chemomechanical caries removal Primary teeth.