title: Four mononuclear platinum(II) complexes: synthesis, DNA/BSA binding, DNA cleavage and cytotoxicity.
abstract: Four new platinum(II) complexes: PtIIL1·H2O (C1, H2L1 = C20H16N2O2), PtIIL2Cl2(C2, L2 = C22H16N2O2), PtIIL3Cl2·H2O (C3, L3 = C20H16N2), PtIIL4Cl2·0.4H2O (C4, L4 = C18H14N4) have been synthesized and characterized by using various physico-chemical techniques. The binding interaction of the four platinum(II) complexes C1-C4 with calf thymus (CT)-DNA has been investigated by UV-Vis and fluorescence emission spectrometry. The apparent binding constant (Kapp)values follow the order: C3 > C1 > C2 > C4. In addition, fluorescence spectrometry of bovine serum albumin (BSA) with the four platinum(II) complexes C1-C4 showed that the quenching mechanism might be a static quenching procedure. For C1-C4, the number of binding sites was about one for BSA and the binding constants follow the order: C3 (7.08 × 105M-1) > C1 (2.82 × 105M-1) > C2 (0.85 × 105M-1) > C4 (0.15 × 105M-1). With the single condition change such as absence of an external agent, the DNA cleavage abilities of C3 exhibit remarkable changes. In addition, the cytotoxicity of C3 in vitro on tumor cells lines (MCF-7, HepG2 and HT29) were examined by MTT and showed better antitumor effects on the tested cells.
authors.affiliation: School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, 224051, Jiangsu, People's Republic of China. wangqm@yctu.edu.cn.
authors.forename: Qingming
authors.initials: Q
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, 224051, Jiangsu, People's Republic of China.|Institute of Nautical Medicine, Nantong University, Nantong, 224051, Jiangsu, People's Republic of China.
authors.forename: Lei
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, 224051, Jiangsu, People's Republic of China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, 224051, Jiangsu, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, 224051, Jiangsu, People's Republic of China.
authors.forename: Jialiang
authors.initials: J
authors.lastname: Song
authors.affiliation: School of Pharmacy, Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection, Yancheng Teachers' University, Yancheng, 224051, Jiangsu, People's Republic of China.
authors.forename: Xinhui
authors.initials: X
authors.lastname: Tang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry* / Q000627:therapeutic use; D002417:Cattle; D049109:Cell Proliferation / Q000187:drug effects; D056831:Coordination Complexes / Q000737:chemistry; D004247:DNA / Q000737:chemistry / Q000187:drug effects*; D053837:DNA Cleavage / Q000187:drug effects; D006801:Humans; D017671:Platinum Compounds / Q000138:chemical synthesis* / Q000737:chemistry* / Q000494:pharmacology; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry
chemical_list: D000970:Antineoplastic Agents; D056831:Coordination Complexes; D017671:Platinum Compounds; D012710:Serum Albumin, Bovine; D004247:DNA
keywords: Binding modes; Bovine serum albumin (BSA) binding; DNA binding; Platinum(II) complexes