title: A Biomimetic Silk Fibroin/Sodium Alginate Composite Scaffold for Soft Tissue Engineering.
abstract: A cytocompatible porous scaffold mimicking the properties of extracellular matrices (ECMs) has great potential in promoting cellular attachment and proliferation for tissue regeneration. A biomimetic scaffold was prepared using silk fibroin (SF)/sodium alginate (SA) in which regular and uniform pore morphology can be formed through a facile freeze-dried method. The scanning electron microscopy (SEM) studies showed the presence of interconnected pores, mostly spread over the entire scaffold with pore diameter around 54~532 μm and porosity 66~94%. With significantly better water stability and high swelling ratios, the blend scaffolds crosslinked by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) provided sufficient time for the formation of neo-tissue and ECMs during tissue regeneration. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) results confirmed random coil structure and silk I conformation were maintained in the blend scaffolds. What's more, FI-TR spectra demonstrated crosslinking reactions occurred actually among EDC, SF and SA macromolecules, which kept integrity of the scaffolds under physiological environment. The suitable pore structure and improved equilibrium swelling capacity of this scaffold could imitate biochemical cues of natural skin ECMs for guiding spatial organization and proliferation of cells in vitro, indicating its potential candidate material for soft tissue engineering.
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables, Hubei Engineering University, Xiaogan 432000, People's Republic of China.
authors.forename: Yiyu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Machine Intelligence and Systems Engineering, Faculty of Systems Science and Technology, Akita Prefectural University, Akita 015-0055, Japan.
authors.forename: Jian
authors.initials: J
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Life Science Technology School, Hubei Engineering University, Xiaogan 432000, People's Republic of China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
authors.forename: Zongrui
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
authors.forename: Daiwei
authors.initials: D
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
authors.forename: Yuanjing
authors.initials: Y
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, People's Republic of China.|Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Lin
authors.affiliation: School of Foreign Languages, Wuhan University of Technology, Wuhan 430070, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Machine Intelligence and Systems Engineering, Faculty of Systems Science and Technology, Akita Prefectural University, Akita 015-0055, Japan.
authors.forename: Mamoru
authors.initials: M
authors.lastname: Mizuno
mesh_terms: D000464:Alginates / Q000737:chemistry*; D000818:Animals; D040761:Biomimetic Materials / Q000737:chemistry*; D012831:Bombyx; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival; D005022:Ethyldimethylaminopropyl Carbodiimide / Q000737:chemistry; D005349:Fibroins / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry; D005612:Freeze Drying; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D051379:Mice; D008855:Microscopy, Electron, Scanning; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013696:Temperature; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000464:Alginates; D005456:Fluorescent Dyes; D006603:Hexuronic Acids; D014867:Water; D020723:Glucuronic Acid; D005349:Fibroins; D005022:Ethyldimethylaminopropyl Carbodiimide