title: Effect of Mesenchymal Stem Cells and Platelet-Rich Plasma on the Bone Healing of Ovariectomized Rats.
abstract: We evaluated the efficacy of platelet-rich plasma (PRP) in combination with allogeneic bone marrow mesenchymal stem cells (BMSCs) for the treatment of osteoporotic bone defects in an ovariectomized rat model. By day 42 after injury,in vivomicrocomputed tomography (micro-CT) imaging revealed that bone defects of control rats and ovariectomized rats treated with PRP and BMSCs were completely repaired, whereas those of ovariectomized rats treated with PRP or BMSCs alone exhibited slower healing. Histological data were consistent with these results. We also assessed changes to bone trabeculae in the proximal tibial growth plate. In ovariectomized rats treated with PRP or with a combination of PRP and BMSCs, the trabecular connectivity densities (Conn.D), bone volume ratios (BV/TV), and numbers (Tb.N) in the defect areas increased significantly from day 7 to day 42. These results indicate that PRP treatment enhances bone microarchitecture in osteoporosis. Moreover, expression levels of osteogenesis-specific marker genes including RUNX2, OSX, and OPN were significantly upregulated in rats treated with PRP and BMSCs compared to those of other groups. Thus, we conclude that treatment with PRP combined with BMSCs significantly promotes healing of osteoporotic bone defects. This study provides an alternative strategy for the treatment of osteoporotic bone loss.
authors.affiliation: Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wei
authors.affiliation: Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Chengshuo
authors.identifier: 0000-0001-5730-2390
authors.initials: C
authors.lastname: Huang
authors.affiliation: Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Mingyan
authors.identifier: 0000-0003-4063-8351
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Peng
authors.identifier: 0000-0003-1695-512X
authors.initials: P
authors.lastname: Li
authors.affiliation: Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Xiang
authors.identifier: 0000-0002-0131-7857
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Junchao
authors.identifier: 0000-0001-8555-371X
authors.initials: J
authors.lastname: Kong
authors.affiliation: Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Yanru
authors.identifier: 0000-0001-7708-5610
authors.initials: Y
authors.lastname: Niu
authors.affiliation: Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Rui
authors.identifier: 0000-0003-2087-3100
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Juanhua
authors.initials: J
authors.lastname: Quan
authors.affiliation: Department of Spinal Surgery, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Jinsong
authors.identifier: 0000-0002-8460-7883
authors.initials: J
authors.lastname: Wei
authors.affiliation: Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China.
authors.forename: Jiaqi
authors.identifier: 0000-0002-2306-1805
authors.initials: J
authors.lastname: Chu