title: Identification of lanthanum-specific peptides for future recycling of rare earth elements from compact fluorescent lamps.
abstract: As components of electronic scrap, rare earth minerals are an interesting but little used source of raw materials that are highly important for the recycling industry. Currently, there exists no cost-efficient technology to separate rare earth minerals from an electronic scrap mixture. In this study, phage surface display has been used as a key method to develop peptides with high specificity for particular inorganic targets in electronic scrap. Lanthanum phosphate doped with cerium and terbium as part of the fluorescent phosphors of spent compact fluorescent lamps (CFL) was used as a target material of economic interest to test the suitability of the phage display method to the separation of rare earth minerals. One random pVIII phage library was screened for peptide sequences that bind specifically to the fluorescent phosphor LaPO4:Ce3+,Tb3+(LAP). The library contained at least 100 binding pVIII peptides per phage particle with a diversity of 1 × 109different phage per library. After three rounds of enrichment, a phage clone containing the surface peptide loop RCQYPLCS was found to bind specifically to LAP. Specificity and affinity of the identified phage bound peptide was confirmed by using binding and competition assays, immunofluorescence assays, and zeta potential measurements. Binding and immunofluorescence assays identified the peptide's affinity for the fluorescent phosphor components CAT (CeMgAl11O19:Tb3+) and BAM (BaMgAl10O17:Eu2+). No affinity was found for other fluorescent phosphor components such as YOX (Y2O3:Eu3+). The binding specificity of the RCQYPLCS peptide loop was improved 3-51-fold by using alanine scanning mutagenesis. The identification of peptides with high specificity and affinity for special components in the fluorescent phosphor in CFLs provides a potentially new strategic approach to rare earth recycling. Biotechnol. Bioeng. 2017;114: 1016-1024. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Centre for Blood Research and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada.|Department of Processing, Helmholtz-Zentrum Dresden-Rossendorf, Helmholtz Institute Freiberg for Resource Technology, Bautzner Landstrasse 400, Dresden, 01328, Germany.
authors.forename: Franziska L
authors.initials: FL
authors.lastname: Lederer
authors.affiliation: Norman B. Keevil Institute of Mining Engineering, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Susan B
authors.initials: SB
authors.lastname: Curtis
authors.affiliation: Department of Processing, Helmholtz-Zentrum Dresden-Rossendorf, Helmholtz Institute Freiberg for Resource Technology, Bautzner Landstrasse 400, Dresden, 01328, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Bachmann
authors.affiliation: Norman B. Keevil Institute of Mining Engineering, University of British Columbia, Vancouver, BC, Canada.
authors.forename: W Scott
authors.initials: WS
authors.lastname: Dunbar
authors.affiliation: Centre for Blood Research and Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Ross T A
authors.initials: RT
authors.lastname: MacGillivray
mesh_terms: D000596:Amino Acids; D061505:Cell Surface Display Techniques / Q000379:methods*; D002563:Cerium / Q000032:analysis / Q000737:chemistry; D017743:Equipment Reuse; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism*; D007811:Lanthanum / Q000032:analysis / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D000596:Amino Acids; D005456:Fluorescent Dyes; D010455:Peptides; D002563:Cerium; D007811:Lanthanum
keywords: LaPO4:Ce,Tb; fluorescent phosphors; phage surface display; rare earths; specificity