title: Derivatizing assay for the determination of aldehydes using micellar electrokinetic chromatography.
abstract: In this work, the use of a novel derivatization agent for the determination of aldehydes (in this particular case: formaldehyde, acetaldehyde, propionaldehyde, and valeraldehyde) using micellar electrokinetic chromatography is reported. The derivatization reaction is based on the reaction of aldehydes with benzhydrazide to form the corresponding derivates with maximum absorbance at 250 nm. The experimental conditions of the derivatization reaction as well of the separation were optimized. The adducts were separated with a +22 kV voltage at a temperature of 29°C. The adducts' separation was performed in less than 14 min using as the running buffer a mixture containing 110 mmol/L of sodium dodecyl sulfate and 27 mmol/L of sodium tetraborate at pH 9.45. Samples were injected using hydrodynamic mode (50 mbar × 5 s). The calibration curves were linear up to 15.0 mg/L with r2above 0.99. Intra and inter-day precisions were in average 3 and 4%, respectively, and recoveries were in average of 95%. Limits of detection and quantification were around 0.5 and 1.5 mg/L, respectively. The developed method was successfully applied in the analysis of low molar weight aldehydes in yogurt and vinegar samples.
authors.affiliation: Federal University of São Carlos, Sorocaba, SP, Brazil.
authors.forename: Tiago Augusto
authors.initials: TA
authors.lastname: Donegatti
authors.affiliation: REQUIMTE/LAQV, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto (FCUP), Porto, Portugal.
authors.forename: Luís Moreira
authors.initials: LM
authors.lastname: Gonçalves
authors.affiliation: Federal University of São Carlos, Sorocaba, SP, Brazil.
authors.forename: Elisabete Alves
authors.initials: EA
authors.lastname: Pereira
mesh_terms: D019342:Acetic Acid / Q000032:analysis; D000447:Aldehydes / Q000032:analysis* / Q000737:chemistry*; D020374:Chromatography, Micellar Electrokinetic Capillary / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D006834:Hydrazines / Q000737:chemistry; D057230:Limit of Detection; D016014:Linear Models; D015203:Reproducibility of Results; D015014:Yogurt / Q000032:analysis
chemical_list: D000447:Aldehydes; D006834:Hydrazines; C006712:benzoylhydrazine; D019342:Acetic Acid
keywords: benzohydrazine; benzoylhydrazine; capillary electrophoresis; food analysis; food quality