title: Molecular dimensions and structural features of neutral polysaccharides from the seed mucilage of Hyptis suaveolens L.
abstract: The seed mucilage of Hyptis suaveolens L. includes acid - and neutral heteropolysaccharides in a ratio of about 1:1. The anionic charged fraction responsible for swelling and viscous behaviour possesses an average molar mass of Mw=350kg/mol, Mn=255kg/mol. The neutral polysaccharide fraction shows an average molar mass of Mw=47kg/mol and Mn=28kg/mol and is composed of d-Galp-, d-Glcp- and d-Manp residues in a molar ratio of about 3:2:1. The structural features present galactoglucan (30%) and galactoglucomannan (70%) with a high level of terminal β-linked d-Galp residues (18%). Structural details of galactoglucomannan are derived by combined enzymatic and chemical methods as well as NMR spectroscopy. Sequences of octa/nonasaccharide β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp-(1→4)-β-d-Glcp-(1→4)-β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp and lower mass tetrasaccharide repeating units β-d-Glcp-(1→4)[β-d-Galp-(1→2)-α-d-Galp-(1→6)]-β-d-Manp were found. The level of the prebiotic activity is related to the availability of β-linked d-Galp residues in the side chains of the molecules.
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria. Electronic address: werner.praznik@univie.ac.at.
authors.forename: Werner
authors.initials: W
authors.lastname: Praznik
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
authors.forename: Andrea
authors.initials: A
authors.lastname: Čavarkapa
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
authors.forename: Frank M
authors.initials: FM
authors.lastname: Unger
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
authors.forename: Renate
authors.initials: R
authors.lastname: Loeppert
authors.affiliation: Department of Pharmaceutical Chemistry - Division of Drug Synthesis, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Holzer
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
authors.forename: Helmut
authors.initials: H
authors.lastname: Viernstein
authors.affiliation: Department of Pharmaceutical Technology and Biopharmaceutics, University of Vienna, Althanstrasse 14, A-1090 Vienna, Austria.
authors.forename: Monika
authors.initials: M
authors.lastname: Mueller
mesh_terms: D005685:Galactans / Q000737:chemistry; D005936:Glucans / Q000737:chemistry; D031331:Hyptis / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008351:Mannans / Q000737:chemistry; D015394:Molecular Structure; D008970:Molecular Weight; D009844:Oligosaccharides / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*
chemical_list: D005685:Galactans; D005936:Glucans; D008351:Mannans; D009844:Oligosaccharides; D011134:Polysaccharides; D011135:Polysaccharides, Bacterial; C443804:galactoglucomannan; C093768:galactoglucan
keywords: Galactoglucan; Galactoglucomannan; Hyptis suaveolens L.; Methylation analysis; Mucilage; NMR spectroscopy; Polysaccharide