title: Modulation of hemoglobin dynamics by an allosteric effector.
abstract: Hemoglobin (Hb) is an extensively studied paradigm of proteins that alter their function in response to allosteric effectors. Models of its action have been used as prototypes for structure-function relationships in many proteins, and models for the molecular basis of its function have been deeply studied and extensively argued. Recent reports suggest that dynamics may play an important role in its function. Relatively little is known about the slow, correlated motions of hemoglobin subunits in various structural states because experimental and computational strategies for their characterization are challenging. Allosteric effectors such as inositol hexaphosphate (IHP) bind to both deoxy-Hb and HbCO, albeit at different sites, leading to a lowered oxygen affinity. The manner in which these effectors impact oxygen binding is unclear and may involve changes in structure, dynamics or both. Here we use neutron spin echo measurements accompanied by wide-angle X-ray scattering to show that binding of IHP to HbCO results in an increase in the rate of coordinated motions of Hb subunits relative to one another with little if any change in large scale structure. This increase of large-scale dynamics seems to be coupled with a decrease in the average magnitude of higher frequency modes of individual residues. These observations indicate that enhanced dynamic motions contribute to the functional changes induced by IHP and suggest that they may be responsible for the lowered oxygen affinity triggered by these effectors.
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, Illinois, 60439.
authors.forename: Jyotsana
authors.initials: J
authors.lastname: Lal
authors.affiliation: Institut Laue-Langevin, CS 20156, 38042 Grenoble Cedex 9, France.
authors.forename: Marco
authors.initials: M
authors.lastname: Maccarini
authors.affiliation: Institut Laue-Langevin, CS 20156, 38042 Grenoble Cedex 9, France.
authors.forename: Peter
authors.initials: P
authors.lastname: Fouquet
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, 15213.
authors.forename: Nancy T
authors.initials: NT
authors.lastname: Ho
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, Pittsburgh, Pennsylvania, 15213.
authors.forename: Chien
authors.initials: C
authors.lastname: Ho
authors.affiliation: Biosciences Division, Argonne National Laboratory, Argonne, Illinois, 60439.|Department of Bioengineering, Northeastern University, Boston, Massachusetts, 02115.
authors.forename: Lee
authors.initials: L
authors.lastname: Makowski
mesh_terms: D000494:Allosteric Regulation; D002263:Carboxyhemoglobin / Q000737:chemistry* / Q000378:metabolism; D006801:Humans; D010833:Phytic Acid / Q000737:chemistry* / Q000378:metabolism; D011485:Protein Binding; D014961:X-Ray Diffraction
chemical_list: D010833:Phytic Acid; D002263:Carboxyhemoglobin
keywords: X-ray solution scattering; hemoglobin; neutron spin echo; protein dynamics