title: Polyethylene oxide (PEO)-hyaluronic acid (HA) nanofibers with kanamycin inhibits the growth of Listeria monocytogenes.
abstract: Listeria monocytogenes is well known to cause prosthetic joint infections in immunocompromised patients. In this study, polyethylene oxide (PEO) nanofibers, containing kanamycin and hyaluronic acid (HA), were prepared by electrospinning at a constant electric field of 10kV. PEO nanofibers spun with 0.2% (w/v) HA and 1% (w/v) kanamycin had a smooth, bead-free structure at 30-35% relative humidity. The average diameter of the nanofibers was 83±20nm. Attenuated total reflectance (ATR)-Fourier transform infrared (FTIR) spectroscopy indicated that kanamycin was successfully incorporated into PEO/HA matrix. The presence of kanamycin affects the thermal properties of PEO/HA nanofibers, as shown by differential scanning calorimetry (DSC) and thermogravimetric analyses (TGA). The kanamycin-PEO-HA nanofibers (1mg; 47±3μg kanamycin) inhibited the growth of L. monocytogenes EDGe by 62%, as compared with PEO-HA nanofibers, suggesting that it may be used to coat prosthetic implants to prevent secondary infections.
authors.affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.
authors.forename: J J
authors.initials: JJ
authors.lastname: Ahire
authors.affiliation: Department of Chemistry and Polymer Science, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.
authors.forename: D D
authors.initials: DD
authors.lastname: Robertson
authors.affiliation: Department of Chemistry and Polymer Science, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa.
authors.forename: A J
authors.initials: AJ
authors.lastname: van Reenen
authors.affiliation: Department of Microbiology, University of Stellenbosch, 7602 Matieland (Stellenbosch), South Africa. Electronic address: LMTD@sun.ac.za.
authors.forename: L M T
authors.initials: LM
authors.lastname: Dicks
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage* / Q000737:chemistry; D004305:Dose-Response Relationship, Drug; D004338:Drug Combinations; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage* / Q000737:chemistry; D007612:Kanamycin / Q000008:administration & dosage* / Q000737:chemistry; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000254:growth & development; D057139:Nanofibers / Q000008:administration & dosage* / Q000737:chemistry; D011092:Polyethylene Glycols / Q000008:administration & dosage* / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D004338:Drug Combinations; D011092:Polyethylene Glycols; D007612:Kanamycin; D006820:Hyaluronic Acid
keywords: Hyaluronic acid; Kanamycin; L. monocytogenes; Nanofibers; Polyethylene oxide