title: Size-exclusion chromatography using core-shell particles.
abstract: Size-exclusion chromatography (SEC) is an indispensable technique for the separation of high-molecular-weight analytes and for determining molar-mass distributions. The potential application of SEC as second-dimension separation in comprehensive two-dimensional liquid chromatography demands very short analysis times. Liquid chromatography benefits from the advent of highly efficient core-shell packing materials, but because of the reduced total pore volume these materials have so far not been explored in SEC. The feasibility of using core-shell particles in SEC has been investigated and contemporary core-shell materials were compared with conventional packing materials for SEC. Columns packed with very small core-shell particles showed excellent resolution in specific molar-mass ranges, depending on the pore size. The analysis times were about an order of magnitude shorter than what could be achieved using conventional SEC columns.
authors.affiliation: University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands; TI-COAST, Science Park 904, 1098 XH Amsterdam, The Netherlands. Electronic address: B.W.J.Pirok@uva.nl.
authors.forename: Bob W J
authors.initials: BW
authors.lastname: Pirok
authors.affiliation: University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Pascal
authors.initials: P
authors.lastname: Breuer
authors.affiliation: University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Serafine J M
authors.initials: SJ
authors.lastname: Hoppe
authors.affiliation: Phenomenex Inc., 411 Madrid Ave., Torrance, CA 90501, USA.
authors.forename: Mike
authors.initials: M
authors.lastname: Chitty
authors.affiliation: Phenomenex Inc., 411 Madrid Ave., Torrance, CA 90501, USA.
authors.forename: Emmet
authors.initials: E
authors.lastname: Welch
authors.affiliation: Phenomenex Inc., 411 Madrid Ave., Torrance, CA 90501, USA.
authors.forename: Tivadar
authors.initials: T
authors.lastname: Farkas
authors.affiliation: University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Sjoerd
authors.initials: S
authors.lastname: van der Wal
authors.affiliation: University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands; DSM Coating Resins, Sluisweg 12, 5145 PE Waalwijk, The Netherlands.
authors.forename: Ron
authors.initials: R
authors.lastname: Peters
authors.affiliation: University of Amsterdam, van't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Science Park 904, 1098 XH Amsterdam, The Netherlands.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Schoenmakers
mesh_terms: D002850:Chromatography, Gel / Q000295:instrumentation* / Q000379:methods*; D008970:Molecular Weight; D016062:Porosity; D013997:Time Factors
keywords: Core-shell particles; Packing resolution factor; Sec; Size-exclusion chromatography; Specific resolution; Uhplc