title: TRANSPARENT TESTA GLABRA 1 ubiquitously regulates plant growth and development from Arabidopsis to foxtail millet (Setaria italica).
abstract: TRANSPARENT TESTA GLABRA 1 of Arabidopsis thaliana (AtTTG1) is a WD40 repeat transcription factor that plays multiple roles in plant growth and development, particularly in seed metabolite production. In the present study, to determine whether SiTTG1 of the phylogenetically distant monocot foxtail millet (Setaria italica) has similar functions, we used transgenic Arabidopsis and Nicotiana systems to explore its activities. We found that SiTTG1 functions as a transcription factor. Overexpression of the SiTTG1 gene rescued many of the mutant phenotypes in Arabidopsis ttg1-13 plants. Additionally, SiTTG1 overexpression fully corrected the reduced expression of mucilage biosynthetic genes, and the induced expression of genes involved in accumulation of seed fatty acids and storage proteins in developing seeds of ttg1-13 plants. Ectopic expression of SiTTG1 restored the sensitivity of the ttg1-13 mutant to salinity and high glucose stresses during germination and seedling establishment, and restored altered expression levels of some stress-responsive genes in ttg1-13 seedlings to the wild type level under salinity and glucose stresses. Our results provide information that will be valuable for understanding the function of TTG1 from monocot to dicot species and identifying a promising target for genetic manipulation of foxtail millet to improve the amount of seed metabolites.
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Kaige
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Shuanghui
authors.initials: S
authors.lastname: Qi
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Changyu
authors.initials: C
authors.lastname: Jin
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Chenhao
authors.initials: C
authors.lastname: Gao
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Duan
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Baili
authors.initials: B
authors.lastname: Feng
authors.affiliation: College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: cmx786@163.com.
authors.forename: Mingxun
authors.initials: M
authors.lastname: Chen
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D005227:Fatty Acids / Q000378:metabolism; D010802:Phylogeny; D063245:Plant Development / Q000235:genetics*; D030821:Plants, Genetically Modified / Q000254:growth & development; D012639:Seeds / Q000378:metabolism; D016415:Sequence Alignment; D020539:Sequence Analysis, Protein; D032983:Setaria Plant / Q000235:genetics* / Q000254:growth & development; D013312:Stress, Physiological; D014026:Nicotiana / Q000235:genetics; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology*
chemical_list: D029681:Arabidopsis Proteins; D005227:Fatty Acids; C121067:TTG1 protein, Arabidopsis; D014157:Transcription Factors
keywords: Arabidopsis; Foxtail millet; Seed metabolite; Seedling establishment; SiTTG1