title: Immunogenicity of a DNA Vaccine Encoding Ag85a-Tb10.4 Antigens from Mycobacterium Tuberculosis.
abstract: BACKGROUND
  Tuberculosis is a life threatening disease that is partially prevented by BCG vaccine. Development of more effective vaccines is an urgent priority in TB control. Ag85a and Tb10.4 are the members of culture filter protein (CFP) of M. tuberculosis that have high immunogenicity.
  
  
  OBJECTIVE
  To analyze the immunogenicity of Ag85a-Tb10.4 DNA vaccine by enzyme-linked immunosorbent assay (ELISA).
  
  
  METHODS
  In this study a previously described plasmid DNA vaccine encoding Ag85a-Tb10.4 was used to examine its capability in the stimulation of immune responses in an animal model. Female BALB/c mice were vaccinated with 100 μg of purified recombinant vector intramuscularly 3 times at two-week intervals and the levels of five cytokines including IFN-γ, IL-12, IL-4, IL-10 and TGF-β were measured.
  
  
  RESULTS
  The levels of IFN-γ and IL-12 for the mice following immunization with Ag85A-Tb10.4 was significantly greater than that of the BCG and control group (p<0.05). However, there was no significant difference in the levels of IL-4, IL-10 and TGF-β between groups.
  
  
  CONCLUSIONS
  IFN-γ and IL-12 Th1 cytokines increased significantly in mice vaccinated with Ag85a-TB10.4 DNA vaccine in comparison to the control and BCG groups. Our results may serve as a groundwork for further research into the prevention and treatment of tuberculosis.
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Meshkat
authors.forename: Amir
authors.initials: A
authors.lastname: Teimourpour
authors.forename: Samira
authors.initials: S
authors.lastname: Rashidian
authors.forename: Mohsen
authors.initials: M
authors.lastname: Arzanlou
authors.forename: Roghayeh
authors.initials: R
authors.lastname: Teimourpour
mesh_terms: D000217:Acyltransferases / Q000235:genetics* / Q000276:immunology*; D000818:Animals; D000942:Antigens, Bacterial / Q000235:genetics* / Q000276:immunology*; D001500:BCG Vaccine / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D009169:Mycobacterium tuberculosis / Q000235:genetics* / Q000276:immunology*; D014376:Tuberculosis / Q000517:prevention & control*; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000942:Antigens, Bacterial; D001500:BCG Vaccine; D016207:Cytokines; D032581:Tuberculosis Vaccines; D019444:Vaccines, DNA; D000217:Acyltransferases; C112580:antigen 85A, Mycobacterium tuberculosis