title: Overexpression of TSPAN8 Promotes Tumor Cell Viability and Proliferation in Nonsmall Cell Lung Cancer.
abstract: BACKGROUND
  Overexpression of TSPAN8 has been involved in several epithelial cancers and TSPAN8 can form a complex with a variety of proteins to participate in several import cellular functions. However, the effects of TSPAN8 in nonsmall cell lung cancer (NSCLC) remain unclear.
  
  
  METHODS
  In this study, the authors determined the expression of TSPAN in several NSCLC cell lines (95C, A549, H1299, and 95D) and human bronchial epithelial (HBE) cells. Furthermore, the authors investigated the biological function of TSPAN8 in NSCLC cell lines using gain-of-function and loss-of-function assays, as well as the underlying mechanisms.
  
  
  RESULTS
  TSPAN8 was found to be overexpressed in NSCLC cells compared with normal HBE cells, of which the expression in H1299 is the highest and, in 95C, it is relatively lowest. Functional assays indicated that knockdown of TSPAN8 in H1299 remarkably reduced cell viability and proliferation, while overexpression of TSPAN8 in 95C dramatically enhanced cell viability and proliferation. In addition, TSPAN8 knockdown led to G1 phase arrest and apoptosis by downregulating CDK2, CDK4, and Cyclin D1 and upregulating Bax and PARP.
  
  
  CONCLUSIONS
  These results provide evidence that TSPAN8 may contribute to the pathogenesis of lung cancer by promoting cell viability and proliferation. TSPAN8 silencing may provide a potential therapeutic intervention for the treatment of NSCLC.
authors.affiliation: Department of Respiration, Yi Shui Central Hospital , Linyi, China .
authors.forename: Zheng
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Department of Respiration, Yi Shui Central Hospital , Linyi, China .
authors.forename: Lijiang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Respiration, Yi Shui Central Hospital , Linyi, China .
authors.forename: Shijun
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Respiration, Yi Shui Central Hospital , Linyi, China .
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Respiration, Yi Shui Central Hospital , Linyi, China .
authors.forename: Zhiguang
authors.initials: Z
authors.lastname: Geng
mesh_terms: D017209:Apoptosis / Q000502:physiology; D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics; D001980:Bronchi / Q000166:cytology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002453:Cell Cycle / Q000502:physiology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D004847:Epithelial Cells / Q000166:cytology; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D015398:Signal Transduction; D059470:Tetraspanins / Q000096:biosynthesis* / Q000235:genetics; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C065609:TSPAN8 protein, human; D059470:Tetraspanins
keywords: NSCLC; TSPAN8; apoptosis; cell cycle; cell proliferation