title: Determination of Morin in Maclura cochinchinensis Heartwood by HPLC.
abstract: The HPLC-DAD method was developed to determine morin content in Maclura cochinchinensis Corner heartwood extract. The chromatographic separation was performed using a Hypersil BDS C18 column, isocratic solvent system of 0.5% acetic acid in water:acetonitrile (80:20) with 1.0 mL/min flow rate and detected at 355 nm. The standard curve of morin was linear in the range of 7-905 μg/mL. The method was precise with intra-day relative standard deviation (RSD) of lower than 1% and 2.06% for inter-day RSD. The method accuracy represented by percent recover was 99.58%. The highly efficient HPLC system developed from this study could detect morin contents in M. cochinchinensis heartwood samples collected from various locations in Thailand in the range of 0.74-1.57% w/w. This developed method provided a useful standardization procedure of M. cochincihinesis materials for further application in pharmacy and other commercial developments.
authors.affiliation: Drug Discovery and Development Center, Thammasat University  (Rangsit Campus), 99 Moo 18 Phaholyothin Road, Khlong Nueng, Khlong Luang, Pathumthani 12120, Thailand.
authors.forename: Sumet
authors.initials: S
authors.lastname: Kongkiatpaiboon
authors.affiliation: Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12120, Thailand.
authors.forename: Parunkul
authors.initials: P
authors.lastname: Tungsukruthai
authors.affiliation: Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12120, Thailand.
authors.forename: Kusuma
authors.initials: K
authors.lastname: Sriyakool
authors.affiliation: Chulabhorn International College of Medicine, Thammasat University, Pathumthani 12120, Thailand.
authors.forename: Kanoktip
authors.initials: K
authors.lastname: Pansuksan
authors.affiliation: Faculty of Pharmacy, Thammasat University, Pathumthani 12120, Thailand.
authors.forename: Amolnat
authors.initials: A
authors.lastname: Tunsirikongkon
authors.affiliation: Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
authors.forename: Hataichanok
authors.initials: H
authors.lastname: Pandith
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis*; D057230:Limit of Detection; D016014:Linear Models; D031624:Maclura / Q000737:chemistry*; D015203:Reproducibility of Results; D013785:Thailand; D014934:Wood / Q000737:chemistry*; D047309:Flavones
chemical_list: D005419:Flavonoids; C008548:morin; D047309:Flavones