title: Exacerbation of oxidative stress during sickle vaso-occlusive crisis is associated with decreased anti-band 3 autoantibodies rate and increased red blood cell-derived microparticle level: a prospective study.
abstract: Painful vaso-occlusive crisis, a hallmark of sickle cell anaemia, results from complex, incompletely understood mechanisms. Red blood cell (RBC) damage caused by continuous endogenous and exogenous oxidative stress may precipitate the occurrence of vaso-occlusive crises. In order to gain insight into the relevance of oxidative stress in vaso-occlusive crisis occurrence, we prospectively compared the expression levels of various oxidative markers in 32 adults with sickle cell anaemia during vaso-occlusive crisis and steady-state conditions. Compared to steady-state condition, plasma levels of free haem, advanced oxidation protein products and myeloperoxidase, RBC caspase-3 activity, as well as the concentrations of total, neutrophil- and RBC-derived microparticles were increased during vaso-occlusive crises, whereas the reduced glutathione content was decreased in RBCs. In addition, natural anti-band 3 autoantibodies levels decreased during crisis and were negatively correlated with the rise in plasma advanced oxidation protein products and RBC caspase-3 activity. These data showed an exacerbation of the oxidative stress during vaso-occlusive crises in sickle cell anaemia patients and strongly suggest that the higher concentration of harmful circulating RBC-derived microparticles and the reduced anti-band 3 autoantibodies levels may be both related to the recruitment of oxidized band 3 into membrane aggregates.
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.
authors.forename: Régine
authors.initials: R
authors.lastname: Hierso
authors.affiliation: CHU de Pointe-à-Pitre, Unité Transversale de la Drépanocytose, Pointe-à-Pitre, Guadeloupe.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Lemonne
authors.affiliation: Instituto de Hematología e Inmunología, La Habana, Cuba.
authors.forename: Rinaldo
authors.initials: R
authors.lastname: Villaescusa
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.|Centre d'Investigation Clinique Antilles Guyane, Inserm/DGOS CIC 1424, Pointe-à-Pitre, Guadeloupe.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Lalanne-Mistrih
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.
authors.forename: Keyne
authors.initials: K
authors.lastname: Charlot
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.|CHU de Pointe-à-Pitre, Unité Transversale de la Drépanocytose, Pointe-à-Pitre, Guadeloupe.
authors.forename: Maryse
authors.initials: M
authors.lastname: Etienne-Julan
authors.affiliation: Centre d'Investigation Clinique Antilles Guyane, Inserm/DGOS CIC 1424, Pointe-à-Pitre, Guadeloupe.
authors.forename: Benoit
authors.initials: B
authors.lastname: Tressières
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.
authors.forename: Yann
authors.initials: Y
authors.lastname: Lamarre
authors.affiliation: CHU de Pointe-à-Pitre, Unité Transversale de la Drépanocytose, Pointe-à-Pitre, Guadeloupe.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Tarer
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.
authors.forename: Yohann
authors.initials: Y
authors.lastname: Garnier
authors.affiliation: Instituto de Hematología e Inmunología, La Habana, Cuba.
authors.forename: Ada Arce
authors.initials: AA
authors.lastname: Hernandez
authors.affiliation: CHU de Pointe-à-Pitre, Service d'accueil des Urgences, Pointe-à-Pitre, Guadeloupe.
authors.forename: Serge
authors.initials: S
authors.lastname: Ferracci
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.|Institut Universitaire de France, Paris, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Connes
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.
authors.forename: Marc
authors.initials: M
authors.lastname: Romana
authors.affiliation: Unité Biologie Intégrée du Globule Rouge, laboratoire d'Excellence GR-Ex, Université des Antilles, Inserm, U 1134, Pointe-à-Pitre, Guadeloupe.
authors.forename: Marie-Dominique
authors.initials: MD
authors.lastname: Hardy-Dessources
mesh_terms: D000293:Adolescent; D000328:Adult; D000755:Anemia, Sickle Cell / Q000097:blood / Q000150:complications*; D001457:Anion Exchange Protein 1, Erythrocyte / Q000276:immunology*; D001157:Arterial Occlusive Diseases; D001323:Autoantibodies / Q000097:blood; D015415:Biomarkers / Q000097:blood; D055252:Cell-Derived Microparticles / Q000276:immunology*; D004912:Erythrocytes / Q000378:metabolism; D005260:Female; D006801:Humans; D008297:Male; D018384:Oxidative Stress; D010146:Pain; D011446:Prospective Studies; D055815:Young Adult
chemical_list: D001457:Anion Exchange Protein 1, Erythrocyte; D001323:Autoantibodies; D015415:Biomarkers
keywords: microparticles; natural anti-band 3 antibodies; oxidative stress; painful vaso-occlusive crisis; sickle cell anaemia