title: Influence of aging on the activity of mice Sca-1+CD31- cardiac stem cells.
abstract: Therapeutic application of cardiac resident stem/progenitor cells (CSC/CPCs) is limited due to decline of their regenerative potential with donor age. A variety of studies have shown that the cardiac aging was the problem of the stem cells, but little is known about the impact of age on the subgroups CSC/CPCs, the relationship between subgroups CSC/CPCs ageing and age-related dysfunction. Here, we studied Sca-1+CD31- subgroups of CSCs from younger(2~3months) and older(22~24months) age mice, biological differentiation was realized using specific mediums for 14 days to induce cardiomyocyte, smooth muscle cells or endothelial cells and immunostain analysis of differentiated cell resulting were done. Proliferation and cell cycle were measured by flow cytometry assay, then used microarray to dissect variability from younger and older mice. Although the number of CSCs was higher in older mice, the advanced age significantly reduced the differentiation ability into cardiac cell lineages and the proliferation ability. Transcriptional changes in Sca-1+CD31- subgroups of CSCs during aging are related to Vitamin B6 metabolism, circadian rhythm, Tyrosine metabolism, Complement and coagulation cascades. Taking together these results indicate that Cardiac resident stem/progenitor cells have significant differences in their proliferative, pluripotency and gene profiles and those differences are age depending.
authors.affiliation: School of Life Sciences, Guangxi Normal University, Guilin, China.|Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, China.|Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Life Sciences, Guangxi Normal University, Guilin, China.|Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, China.|Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, China.
authors.forename: Jinxi
authors.initials: J
authors.lastname: Zhan
authors.affiliation: School of Life Sciences, Guangxi Normal University, Guilin, China.|Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, China.|Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Pu
authors.affiliation: School of Life Sciences, Guangxi Normal University, Guilin, China.|Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, China.|Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Qin
authors.affiliation: School of Life Sciences, Guangxi Normal University, Guilin, China.|Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, China.|Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Life Sciences, Guangxi Normal University, Guilin, China.|Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, China.|Research Center for Biomedical Sciences, Guangxi Normal University, Guilin, China.
authors.forename: Zuping
authors.initials: Z
authors.lastname: Zhou
mesh_terms: D000367:Age Factors; D000375:Aging; D000818:Animals; D000950:Antigens, Ly / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D005260:Female; D020869:Gene Expression Profiling; D016130:Immunophenotyping; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism; D051379:Mice; D032386:Myoblasts, Cardiac / Q000166:cytology / Q000378:metabolism*; D010641:Phenotype; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism*
chemical_list: D000950:Antigens, Ly; C074650:Ly6a protein, mouse; D008565:Membrane Proteins; D019408:Platelet Endothelial Cell Adhesion Molecule-1
keywords: Gerotarget; aging; cardiac resident stem/progenitor cells; differentiation; proliferation