title: In VivoFitness Adaptations of Colistin-Resistant Acinetobacter baumannii Isolates to Oxidative Stress.
abstract: The loss of fitness in colistin-resistant (CR)Acinetobacter baumanniiwas investigated using longitudinal isolates from the same patient. Early CR isolates were outcompeted by late CR isolates for growth in broth and survival in the lungs of mice. Fitness loss was associated with an increased susceptibility to oxidative stress since early CR strains had reducedin vitrosurvival in the presence of hydrogen peroxide and decreased catalase activity compared to that of late CR and colistin-susceptible (CS) strains.
authors.affiliation: Wound Infections Department, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA dr.crystaljones@gmail.com.
authors.forename: Crystal L
authors.initials: CL
authors.lastname: Jones
authors.affiliation: Wound Infections Department, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Shweta S
authors.initials: SS
authors.lastname: Singh
authors.affiliation: Wound Infections Department, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Yonas
authors.initials: Y
authors.lastname: Alamneh
authors.affiliation: Department of Microbial Pathogenesis, University of Maryland School of Dentistry, Baltimore, Maryland, USA.
authors.forename: Leila G
authors.initials: LG
authors.lastname: Casella
authors.affiliation: Department of Microbial Pathogenesis, University of Maryland School of Dentistry, Baltimore, Maryland, USA.
authors.forename: Robert K
authors.initials: RK
authors.lastname: Ernst
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Emil P
authors.initials: EP
authors.lastname: Lesho
authors.affiliation: Multidrug-resistant Organism Repository and Surveillance Network, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Paige E
authors.initials: PE
authors.lastname: Waterman
authors.affiliation: Wound Infections Department, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
authors.forename: Daniel V
authors.initials: DV
authors.lastname: Zurawski
mesh_terms: D000151:Acinetobacter Infections / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology; D040981:Acinetobacter baumannii / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D000222:Adaptation, Physiological / Q000187:drug effects* / Q000235:genetics; D000328:Adult; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D003091:Colistin / Q000494:pharmacology*; D024881:Drug Resistance, Bacterial / Q000235:genetics*; D056084:Genetic Fitness; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D008297:Male; D051379:Mice; D018384:Oxidative Stress; D013997:Time Factors; D014774:Virulence / Q000187:drug effects; D014948:Wounds, Gunshot / Q000188:drug therapy / Q000382:microbiology / Q000473:pathology
chemical_list: D000900:Anti-Bacterial Agents; D006861:Hydrogen Peroxide; D003091:Colistin
keywords: Acinetobacter; ESKAPE; catalase; fitness; mass spectrometry; mouse model; serial isolation; virulence