title: A study on biomimetic immunoassay-capillary electrophoresis method based on molecularly imprinted polymer for determination of trace trichlorfon residue in vegetables.
abstract: Pesticide residue in vegetables is a serious problem that has adverse effects on human health. In our study, we designed and synthesized a molecularly imprinted polymer that can selectively recognize trichlorfon. Using the polymer material as biomimetic antibody, we developed a biomimetic immunoassay-capillary electrophoresis method with improved sensitivity for the detection of trichlorfon. We evaluated the competitive reactions between HRP labeled trichlorfon hapten and free trichlorfon with the biomimetic antibody. Factors that affected the sensitivity of our method were tested in detail. Under optimal conditions, the limit of detection (LOD, IC15) and the sensitivity (IC50) of this method were 0.16mgL-1and 0.13μgL-1for trichlorfon. We used this method to determine the trichlorfon spiked in the kidney bean and cucumber samples with recoveries ranging from 78.8% to 103%. We also detected residual trichlorfons in the leek samples, and these results were verified by gas chromatography method.
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China.
authors.forename: Juxiu
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China. Electronic address: xgqiao@sdau.edu.cn.
authors.forename: Xuguang
authors.initials: X
authors.lastname: Qiao
authors.affiliation: College of Food Science and Engineering, Shandong Agricultural University, Tai'an 271018, People's Republic of China. Electronic address: zhixiangxu@sina.com.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Xu
mesh_terms: D032701:Biomimetics; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005506:Food Contamination / Q000032:analysis; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D054802:Molecular Imprinting; D019697:Onions / Q000737:chemistry; D010573:Pesticide Residues / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry*; D014236:Trichlorfon / Q000737:chemistry*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D010573:Pesticide Residues; D011108:Polymers; D014236:Trichlorfon
keywords: Biomimetic immunoassay; Capillary electrophoresis; Gas chromatography; Molecularly imprinted polymer; Trichlorfon