title: Quantum dot interactions and flow effects in angiogenic zebrafish (Danio rerio) vessels and human endothelial cells.
abstract: Nanoparticle (NP) interactions with biological tissues are affected by the size, shape and surface chemistry of the NPs. Here we use in vivo (zebrafish) and in vitro (HUVEC) models to investigate association of quantum dots (QDs) with endothelial cells and the effect of fluid flow. After injection into the developing zebrafish, circulating QDs associate with endothelium and penetrate surrounding tissue parenchyma over time. Amino-functionalized QDs cluster, interact with cells, and clear more rapidly than carboxy-functionalized QDs in vivo, highlighting charge influences. QDs show stronger accumulation in slow-flowing, small caliber venous vessels than in fast-flowing high caliber arterial vessels. Parallel-plate flow experiments with HUVEC support these findings, showing reduced QD-EC association with increasing flow. In vivo, flow arrest after nanoparticle injection still results in venous accumulation at 18 h. Overall our results suggest that both QD charge and blood flow modulate particle-endothelial cell interactions.
authors.affiliation: Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB, Canada; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada.
authors.forename: Xiao-Yu
authors.initials: XY
authors.lastname: Jiang
authors.affiliation: Cellular and Molecular Bioengineering Research Laboratory, Schulich School of Engineering, University of Calgary, Calgary, AB, Canada.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Sarsons
authors.affiliation: Cellular and Molecular Bioengineering Research Laboratory, Schulich School of Engineering, University of Calgary, Calgary, AB, Canada.
authors.forename: M Juliana
authors.initials: MJ
authors.lastname: Gomez-Garcia
authors.affiliation: Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Department of Chemistry, Faculty of Science, University of Calgary, Calgary, AB, Canada.
authors.forename: David T
authors.initials: DT
authors.lastname: Cramb
authors.affiliation: Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB, Canada; Cellular and Molecular Bioengineering Research Laboratory, Schulich School of Engineering, University of Calgary, Calgary, AB, Canada; Centre for Bioengineering Research and Education, Schulich School of Engineering, University of Calgary, Calgary, AB, Canada; Department of Chemical and Petroleum Engineering, Schulich School of Engineering, University of Calgary, Calgary, AB, Canada; Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada; Libin Cardiovascular Institute of Alberta, University of Calgary, Calgary, AB, Canada.
authors.forename: Kristina D
authors.initials: KD
authors.lastname: Rinker
authors.affiliation: Alberta Children's Hospital Research Institute, University of Calgary, Calgary, AB, Canada; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary, Calgary, AB, Canada. Electronic address: schilds@ucalgary.ca.
authors.forename: Sarah J
authors.initials: SJ
authors.lastname: Childs
mesh_terms: D000180:Acrylic Resins / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D000586:Amination; D000818:Animals; D001783:Blood Flow Velocity; D001808:Blood Vessels / Q000187:drug effects / Q000502:physiology*; D002264:Carboxylic Acids / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D002470:Cell Survival / Q000187:drug effects; D042783:Endothelial Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D011092:Polyethylene Glycols / Q000008:administration & dosage / Q000378:metabolism / Q000633:toxicity; D045663:Quantum Dots / Q000008:administration & dosage / Q000378:metabolism* / Q000633:toxicity; D015027:Zebrafish
chemical_list: D000180:Acrylic Resins; D002264:Carboxylic Acids; D011092:Polyethylene Glycols; C006903:carbopol 940
keywords: Angiogenesis; Nanoparticle; Quantum dots; Shear stress; Uptake; Zebrafish