title: 3D modeling of keloid scars in vitro by cell and tissue engineering.
abstract: Keloids are pathologic scars defined as dermal fibrotic tumors resulting from a disturbance of skin wound healing process. Treatments against keloids are multiple, sometimes empirical and none of them really provides an effective tool for physicians. The lack of effective treatments is correlated with the poor understanding of keloid pathogenesis. To fill this gap, researchers need strong models mimicking keloids as closely as possible. The objective of this study was to establish in vitro a new reconstructed keloid model (RKM), by combining fibroblasts extracted from the three major area of a keloid (center, periphery, non-lesional) in a three-dimensional biomaterial. To this aim, fibroblasts of three keloid locations were extracted and characterized, and then integrated in a hydrated collagen gel matrix during a three-step procedure. The heterogeneity of fibroblasts was assessed according to their proliferative and remodeling capacities. RKMs were further visualized and characterized by both light and scanning electron microscopy. This reconstructed keloid model should be very useful for investigating keloid fibroblasts function in conditions mimicking in vivo situation. Moreover, RKM should also be a suitable model for either drug study and discovery or innovative approaches using medical devices both during cancer and cancer-like disease investigation.
authors.affiliation: Laboratory of Physical Chemistry, Molecular and Cellular Biology, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Center of Excellence for Molecular Imaging, Chiang Mai University, Chiang Mai, Thailand.|Clinical Investigation Center, Inserm CICB 1431, University Hospital of Besançon, 2 Place St Jacques, 25000, Besançon, France.|Inserm UMR 1098, Engineering and Cutaneous Biology Team, Besançon, France.|University of Bourgogne Franche-Comté, SFR FED 4234, Besançon, France.
authors.forename: Dutsadee
authors.initials: D
authors.lastname: Suttho
authors.affiliation: Laboratory of Physical Chemistry, Molecular and Cellular Biology, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Center of Excellence for Molecular Imaging, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Samlee
authors.initials: S
authors.lastname: Mankhetkorn
authors.affiliation: Clinical Investigation Center, Inserm CICB 1431, University Hospital of Besançon, 2 Place St Jacques, 25000, Besançon, France.|University of Bourgogne Franche-Comté, SFR FED 4234, Besançon, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Binda
authors.affiliation: Clinical Investigation Center, Inserm CICB 1431, University Hospital of Besançon, 2 Place St Jacques, 25000, Besançon, France.|University of Bourgogne Franche-Comté, SFR FED 4234, Besançon, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Pazart
authors.affiliation: Clinical Investigation Center, Inserm CICB 1431, University Hospital of Besançon, 2 Place St Jacques, 25000, Besançon, France.|Inserm UMR 1098, Engineering and Cutaneous Biology Team, Besançon, France.|University of Bourgogne Franche-Comté, SFR FED 4234, Besançon, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Humbert
authors.affiliation: Clinical Investigation Center, Inserm CICB 1431, University Hospital of Besançon, 2 Place St Jacques, 25000, Besançon, France. grolin@chu-besancon.fr.|Inserm UMR 1098, Engineering and Cutaneous Biology Team, Besançon, France. grolin@chu-besancon.fr.|University of Bourgogne Franche-Comté, SFR FED 4234, Besançon, France. grolin@chu-besancon.fr.
authors.forename: Gwenaël
authors.initials: G
authors.lastname: Rolin
mesh_terms: D049109:Cell Proliferation; D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism; D005347:Fibroblasts / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D005782:Gels; D006801:Humans; D007627:Keloid / Q000378:metabolism / Q000473:pathology*; D007700:Kinetics; D008297:Male; D008855:Microscopy, Electron, Scanning; D010641:Phenotype; D012867:Skin / Q000378:metabolism / Q000473:pathology* / Q000648:ultrastructure; D023822:Tissue Engineering / Q000379:methods*; D055815:Young Adult
chemical_list: D005782:Gels; D003094:Collagen
keywords: 3D model; Fibroblasts; Keloid scar; Tissue engineering