title: A High Copy Suppressor Screen for Autophagy Defects inSaccharomyces arl1Δ andypt6Δ Strains.
abstract: InSaccharomyces cerevisiae, Arl1 and Ypt6, two small GTP-binding proteins that regulate membrane traffic in the secretory and endocytic pathways, are also necessary for autophagy. To gain information about potential partners of Arl1 and Ypt6 specifically in autophagy, we carried out a high copy number suppressor screen to identify genes that when overexpressed suppress the rapamycin sensitivity phenotype ofarl1Δ andypt6Δ strains at 37°. From the screen results, we selectedCOG4,SNX4,TAX4,IVY1,PEP3,SLT2, andATG5, either membrane traffic or autophagy regulators, to further test whether they can suppress the specific autophagy defects ofarl1Δ andypt6Δ strains. As a result, we identifiedCOG4,SNX4, andTAX4to be specific suppressors for thearl1Δ strain, andIVY1andATG5for theypt6Δ strain. Through this screen, we were able to confirm several membrane traffic and autophagy regulators that have novel relationships with Arl1 and Ypt6 during autophagy.
authors.affiliation: Department of Biology, Georgetown University, Washington, DC 20057.
authors.forename: Shu
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Biology, Georgetown University, Washington, DC 20057 rosenwaa@georgetown.edu.
authors.forename: Anne
authors.identifier: 0000-0002-0144-8515
authors.initials: A
authors.lastname: Rosenwald
mesh_terms: D033942:Adaptor Proteins, Vesicular Transport / Q000235:genetics; D001343:Autophagy / Q000235:genetics*; D000071187:Autophagy-Related Protein 5 / Q000235:genetics; D002352:Carrier Proteins / Q000235:genetics; D004705:Endocytosis / Q000235:genetics; D008565:Membrane Proteins / Q000235:genetics; D026901:Membrane Transport Proteins / Q000235:genetics; D020559:Monomeric GTP-Binding Proteins / Q000235:genetics*; D009154:Mutation; D021381:Protein Transport / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D033921:Vesicular Transport Proteins / Q000235:genetics*
chemical_list: C102268:ATG5 protein, S cerevisiae; D033942:Adaptor Proteins, Vesicular Transport; D000071187:Autophagy-Related Protein 5; C468122:COG4 protein, S cerevisiae; D002352:Carrier Proteins; C475841:IVY1 protein, S cerevisiae; D008565:Membrane Proteins; D026901:Membrane Transport Proteins; D029701:Saccharomyces cerevisiae Proteins; C530061:Tax4 protein, S cerevisiae; D033921:Vesicular Transport Proteins; C487039:ARL1 protein, S cerevisiae; D020559:Monomeric GTP-Binding Proteins; C499307:YPT6 protein, S cerevisiae
keywords: ARL1; Saccharomyces cerevisiae; YPT6; autophagy; rapamycin