title: Regressive Effect of Myricetin on Hepatic Steatosis in Mice Fed a High-Fat Diet.
abstract: Myricetin is an effective antioxidant in the treatment of obesity and obesity-related metabolic disorders. The objective of this study was to explore the regressive effect of myricetin on pre-existing hepatic steatosis induced by high-fat diet (HFD). C57BL/6 mice were fed either a standard diet or a HFD for 12 weeks and then half of the mice were treated with myricetin (0.12% in the diet,w/w) while on their respective diets for further 12 weeks. Myricetin treatment significantly alleviated HFD-induced steatosis, decreased hepatic lipid accumulation and thiobarbituric acid reactive substance (TBARS) levels, and increased antioxidative enzyme activities, including catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GPx) activities. Microarray analysis of hepatic gene expression profiles showed that myricetin significantly altered the expression profiles of 177 genes which were involved in 12 biological pathways, including the peroxisome proliferator activated receptor (PPAR) signaling pathway and peroxisome. Further research indicated that myricetin elevated hepatic nuclear Nrf2 translocation, increased the protein expression of heme oxygenase-1 (HO-1) and NAD(P)H quinone dehydrogenase 1 (NQO1), reduced the protein expression of PPARγ, and normalized the expressions of genes that were involved in peroxisome and the PPAR signaling pathway. Our data indicated that myricetin might represent an effective therapeutic agent to treat HFD-induced hepatic steatosis via activating the Nrf2 pathway and the PPAR signaling pathway.
authors.affiliation: Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China. sfxia2015@163.com.|State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. sfxia2015@163.com.
authors.forename: Shu-Fang
authors.initials: SF
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. 1601050217@163.com.
authors.forename: Guo-Wei
authors.initials: GW
authors.lastname: Le
authors.affiliation: COFCO Corporation Oilseeds Processing Division, Beijing 100020, China. wpeng@cofco.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China. yuyuqiu1102@aliyun.com.
authors.forename: Yu-Yu
authors.initials: YY
authors.lastname: Qiu
authors.affiliation: Wuxi School of Medicine, Jiangnan University, Wuxi 214122, China. doctoryuyu@126.com.
authors.forename: Yu-Yu
authors.initials: YY
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. tangxue@jiangnan.edu.cn.
authors.forename: Xue
authors.initials: X
authors.lastname: Tang
mesh_terms: D021581:Active Transport, Cell Nucleus; D000818:Animals; D000975:Antioxidants / Q000627:therapeutic use*; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects; D019587:Dietary Supplements; D004195:Disease Models, Animal; D005419:Flavonoids / Q000627:therapeutic use*; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism; D008099:Liver / Q000201:enzymology / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008565:Membrane Proteins / Q000819:agonists / Q000235:genetics / Q000378:metabolism; D008810:Mice, Inbred C57BL; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000235:genetics / Q000378:metabolism; D065626:Non-alcoholic Fatty Liver Disease / Q000097:blood / Q000178:diet therapy* / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress; D047495:PPAR gamma / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D020675:Peroxisomes / Q000378:metabolism / Q000473:pathology; D011897:Random Allocation; D015398:Signal Transduction
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D005419:Flavonoids; D008565:Membrane Proteins; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D047495:PPAR gamma; C040015:myricetin; D051547:Heme Oxygenase-1; C496542:Hmox1 protein, mouse; D016660:NAD(P)H Dehydrogenase (Quinone); C491732:Nqo1 protein, mouse
keywords: Nrf2; PPARγ; hepatic steatosis; myricetin; oxidative stress