title: Inhibition of bovine platelets aggregation in response toHyalomma anatolicumsalivary gland proteins/peptides.
abstract: OBJECTIVE
  Ticks are obligate ectoparasites that have an impact on wide range of vertebrates and also act as a potential vector for the transmission of tropical theileriosis, babesiosis, etc., causing significant loss to livestock production worldwide. While feeding, they introduce their saliva containing different bioactive molecules into the host. These molecules have the capability to counteract the host hemostatic mechanism to suck host blood successfully. Therefore, the study was aimed to isolate anti-platelet aggregating peptides from salivary gland extract (SGE) ofHyalomma anatolicumticks, a commonly available tick in India.
  
  
  METHODS
  FemaleH. anatolicumsalivary glands were dissected out and SGE was prepared by homogenizing it in a suitable buffer under ice. Extract so obtained was fractionated by gel filtration chromatography using Sephacryl S-200 column. Total protein concentration in fractions was estimated and bovine platelets were isolated, stimulated with thrombin (positive control), treated with Gly-Pro-Arg-Pro amide (negative control) and with salivary gland fractions for identification of proteins/peptides having anti-platelet aggregating activities.
  
  
  RESULTS
  Proteins/peptides present in various salivary gland fractions inhibited the bovine platelet aggregation and the percent inhibition ranged between 33% and 35.8%.
  
  
  CONCLUSIONS
  The results suggests that the fractions ofH. anatolicumsalivary glands possess thrombin-induced anti-platelet aggregating activity and which could be further exploited for raising anti-tick vaccine and also for therapeutic purpose.
authors.affiliation: Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India.
authors.lastname: Surbhi
authors.affiliation: Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India.
authors.forename: Nirmal
authors.initials: N
authors.lastname: Sangwan
authors.affiliation: Department of Veterinary Parasitology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India.
authors.forename: Arun K
authors.initials: AK
authors.lastname: Sangwan
authors.affiliation: Department of Veterinary Physiology and Biochemistry, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India.
authors.forename: Vijender
authors.initials: V
authors.lastname: Singh
authors.affiliation: Department of Veterinary Medicine, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar - 125 004, Haryana, India.
authors.forename: Ankit
authors.initials: A
authors.lastname: Kumar
keywords: Hyalomma anatolicum salivary gland; anti-platelet aggregating proteins/peptides; gel filtration chromatography; thrombin