title: Detection of heart failure-related biomarker in whole blood with graphene field effect transistor biosensor.
abstract: Since brain natriuretic peptide (BNP) has become internationally recognized biomarkers in the diagnosis and prognosis of heart failure (HF), it is highly desirable to search for a novel sensing tool for detecting the patient's BNP level at the early stage. Here we report a platinum nanoparticles (PtNPs)-decorated reduced graphene oxide (rGO) field effect transistor (FET) biosensor coupled with a microfilter system for label-free and highly sensitive detection of BNP in whole blood. The PtNPs-decorated rGO FET sensor was obtained by drop-casting rGO onto the pre-fabricated FET chip and subsequently assembling PtNPs on the graphene surface. After anti-BNP was bound to the PtNPs surface, BNP was successfully detected by the anti-BNP immobilized FET biosensor. It was found that the developed FET biosensor was able to achieve a low detection limitation of 100fM. Moreover, BNP was successfully detected in human whole blood sample treated by a custom-made microfilter, suggesting the sensor's capability of working in a complex sample matrix. The developed FET biosensor provides a new sensing platform for protein detection, showing its potential applications in clinic sample.
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, PR China; School of Information Engineering, Wuhan Technology and Business University, 1 Huangjia Lake West Road, Wuhan 430065, PR China.
authors.forename: Yong-Min
authors.initials: YM
authors.lastname: Lei
authors.affiliation: Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University, No.5 Yiheyuan Road Haidian District, Beijing 100871, PR China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Xiao
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, PR China.
authors.forename: Yu-Tao
authors.initials: YT
authors.lastname: Li
authors.affiliation: School of Pharmacy, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Xu
authors.affiliation: Teaching and Research Office of Forensic Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory for the Physics and Chemistry of Nanodevices, Department of Electronics, Peking University, No.5 Yiheyuan Road Haidian District, Beijing 100871, PR China. Electronic address: zyzhang@pku.edu.cn.
authors.forename: Zhi-Yong
authors.initials: ZY
authors.lastname: Zhang
authors.affiliation: School of Laboratory Medicine, Hubei University of Chinese Medicine, 1 Huangjia Lake West Road, Wuhan 430065, PR China. Electronic address: zhanggj@hbtcm.edu.cn.
authors.forename: Guo-Jun
authors.initials: GJ
authors.lastname: Zhang
mesh_terms: D015415:Biomarkers / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D004867:Equipment Design; D006108:Graphite / Q000737:chemistry*; D006333:Heart Failure / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D020097:Natriuretic Peptide, Brain / Q000097:blood*; D010087:Oxides / Q000737:chemistry; D010984:Platinum / Q000737:chemistry*; D014173:Transistors, Electronic
chemical_list: D015415:Biomarkers; D010087:Oxides; D020097:Natriuretic Peptide, Brain; D010984:Platinum; D006108:Graphite
keywords: Brain natriuretic peptide; Detection; Field effect transistor biosensor; Human whole blood; Platinum nanoparticles; Reduced graphene oxide