title: Characterization of Fractional Polysaccharides from Gleditsia sinensis and Gleditsia microphylla Gums.
abstract: The seeds ofGleditsia sinensisandGleditsia microphylla, widespread in China, are an important source of galactomannans.G. sinensisgum (GSG) andG. microphyllagum (GMG) were purified and precipitated using different concentrations of ethanol and isopropanol. The GSG and GMG, precipitated in different stages, presented different characteristics, including polymer recovery, mannose/galactose ratio, chemical composition, molecular weight, and morphological appearance. The galactomannan recovery of GSG and GMG in 33.3% ethanol was 81.7% and 82.5%, respectively, while that in 28.8% isopropanol was 81.3% and 82.9%, respectively. To achieve similar precipitation efficiency, the amount of isopropanol should be lower than that of ethanol because of the lower dielectric constant of isopropanol (20 vs. 25 for ethanol). The precipitation behavior of galactomannans in polar organic solvents was dependent on the molecular structures and properties of the solvent. A higher mannose/galactose ratio and a higher molecular weight was obtained in a lower concentration of alcohols.
authors.affiliation: MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China. liuyt0321@163.com.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China. zwa728@sina.com.
authors.forename: Zhenglong
authors.initials: Z
authors.lastname: Xu
authors.affiliation: MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China. ziyuanbeijing@163.com.
authors.forename: Weian
authors.initials: W
authors.lastname: Zhang
authors.affiliation: MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China. duanjiu99@163.com.
authors.forename: Jiufang
authors.initials: J
authors.lastname: Duan
authors.affiliation: MOE Engineering Research Center of Forestry Biomass Materials and Bioenergy, Beijing Forestry University, Beijing 100083, China. jiangjx2004@hotmail.com.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Nanjing Institute for the Comprehensive Utilization of Wild Plant, Nanjing 210042, China. sdafeng@163.com.
authors.forename: Dafeng
authors.initials: D
authors.lastname: Sun
mesh_terms: D019840:2-Propanol / Q000737:chemistry; D002850:Chromatography, Gel; D000431:Ethanol / Q000737:chemistry; D005690:Galactose / Q000032:analysis / Q000031:analogs & derivatives; D031501:Gleditsia / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D008358:Mannose / Q000032:analysis; D008970:Molecular Weight; D053149:Plant Gums / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D014783:Viscosity
chemical_list: D008351:Mannans; D053149:Plant Gums; C012990:galactomannan; D000431:Ethanol; D019840:2-Propanol; D008358:Mannose; D005690:Galactose
keywords: Gleditsia microphylla; Gleditsia sinensis; ethanol; fractionation; isopropanol