title: Detection of Hepatitis B Virus Particles Released from Cultured Cells by Particle Gel Assay.
abstract: The culture fluid of HBV replicating cells contains a mixture of viral particles with different structural and genetic components, including enveloped infectious virions, genome-free virion, envelope-only subviral particles, and nonenveloped naked capsids. Based on their different physical and chemical properties, the enveloped and nonenveloped particles can be separated by the native agarose gel electrophoresis and transferred onto a positively charged microporous membrane, then the virus particle-associated protein components and nucleic acid content can be detected by antibody-based enzyme immunoassay (EIA) and hybridization, respectively. Such convenient experimental procedure is called HBV particle assay and described in detail in this chapter. The particle gel assay can be used to study viral and host regulations of HBV virus morphogenesis and egress, and for antiviral assessment of HBV inhibitors as well.
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN, 46202, USA.
authors.forename: Ran
authors.initials: R
authors.lastname: Yan
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN, 46202, USA.
authors.forename: Dawei
authors.initials: D
authors.lastname: Cai
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN, 46202, USA.
authors.forename: Yuanjie
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Microbiology and Immunology, Indiana University School of Medicine, 635 Barnhill Drive, Indianapolis, IN, 46202, USA. haitguo@iupui.edu.
authors.forename: Haitao
authors.initials: H
authors.lastname: Guo
mesh_terms: D002460:Cell Line; D002478:Cells, Cultured; D004279:DNA, Viral; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D009693:Nucleic Acid Hybridization / Q000379:methods; D014771:Virion; D057074:Virus Release
chemical_list: D004279:DNA, Viral
keywords: HBV particles; Particle gel assay