title: Physiochemical and biological characterization of novel exopolysaccharide produced by Bacillus tequilensis FR9 isolated from chicken.
abstract: Hetero-exopolysaccharide secreted by Bacillus tequilensis FR9 (BtqEPS), was produced and fractioned on DEAE-Sepharose and phenyl sepharose CL-6B column. HPLC analysis revealed the existence of five monosaccharides including glucose, galactose, mannose, arabinose and xylose. FT-IR spectroscopy confirmed the presence of carboxyl and hydroxyl groups.1H NMR and13C NMR spectra analysis showed the presence of α and β-d-(+)-glucose residues and glycosidic linkages. The fibrous, porous nature and elemental composition (C, O, N, Cl, Na, P, S) of BtqEPS was inferred from SEM and EDX analysis. AFM proved that the micro-structure of BtqEPS is compact and rough. XRD analysis confirmed the amorphous nature of BtqEPS with 15.6% crystallinity index. TGA curve indicated the degradation temperature (Td) of 239.72°C. Furthermore, BtqEPS exhibited antioxidant effects by scavenging reactive oxygen species (ROS) and demonstrated strong reducing power. All the above findings on BtqEPS paves way to find novel insights of its potential applications in industries.
authors.affiliation: Department of Biology, The Gandhigram Rural Institute-Deemed University, Gandhigram, 624302, Tamilnadu, India. Electronic address: rizm_green@yahoo.com.
authors.forename: Rizwana Parveen
authors.initials: RP
authors.lastname: Rani
authors.affiliation: Biodiversity Research Center, Academia Sinica, Taipei 115, Taiwan. Electronic address: anandharaj49@gmail.com.
authors.forename: Marimuthu
authors.initials: M
authors.lastname: Anandharaj
authors.affiliation: Institute of Atomic and Molecular Sciences, Academia Sinica, Taipei 115, Taiwan. Electronic address: psabhaphd1990@yahoo.com.
authors.forename: Palani
authors.initials: P
authors.lastname: Sabhapathy
authors.affiliation: Department of Biology, The Gandhigram Rural Institute-Deemed University, Gandhigram, 624302, Tamilnadu, India. Electronic address: david_gribiology@rediffmail.com.
authors.forename: Abraham David
authors.initials: AD
authors.lastname: Ravindran
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D001407:Bacillus / Q000737:chemistry* / Q000302:isolation & purification / Q000502:physiology; D055598:Chemical Phenomena; D002645:Chickens / Q000382:microbiology*; D005609:Free Radicals / Q000737:chemistry; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D011135:Polysaccharides, Bacterial / Q000737:chemistry* / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000737:chemistry
chemical_list: D000975:Antioxidants; D005609:Free Radicals; D009005:Monosaccharides; D011135:Polysaccharides, Bacterial; D017382:Reactive Oxygen Species
keywords: Antioxidant activity; Bacillus tequilensis; Exopolysaccharide