title: Strategies on process engineering of chondrocyte culture for cartilage tissue regeneration.
abstract: The current work is an attempt to study the strategies for cartilage tissue regeneration using porous scaffold in wavy walled airlift bioreactor (ALBR). Novel chitosan, poly (L-lactide) and hyaluronic acid based composite scaffold were prepared. The scaffolds were cross-linked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide, N-hydroxysuccinimide and chondroitin sulfate to obtain interconnected 3D microstructure showing excellent biocompatibility, higher cellular differentiation and increased stability. The surface morphology and porosity of the scaffolds were analyzed using scanning electron microscopy (SEM) and mercury intrusion porosimeter and optimized for chondrocyte regeneration. The study shows that the scaffolds were highly porous with pore size ranging from 48 to 180 µm and the porosities in the range 80-92%. Swelling and in vitro degradation studies were performed for the composite scaffolds; by increasing the chitosan: HA ratio in the composite scaffolds, the swelling property increases and stabilizes after 24 h. There was controlled degradation of composite scaffolds for 4 weeks. The uniform chondrocyte distribution in the scaffold using various growth modes in the shake flask and ALBR was studied by glycosaminoglycans (GAG) quantification, MTT assay and mixing time evaluation. The cell culture studies demonstrated that efficient designing of ALBR increases the cartilage regeneration as compared to using a shake flask. The free chondrocyte microscopy and cell attachment were performed by inverted microscope and SEM, and from the study it was confirmed that the cells uniformly attached to the scaffold. This study focuses on optimizing strategies for the culture of chondrocyte using suitable scaffold for improved cartilage tissue regeneration.
authors.affiliation: School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221005, India.
authors.forename: Sarada Prasanna
authors.initials: SP
authors.lastname: Mallick
authors.affiliation: Department of Orthopaedics, Institute of Medical Sciences, Banaras Hindu University, Varanasi, 221005, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Rastogi
authors.affiliation: School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221005, India.
authors.forename: Satyavrat
authors.initials: S
authors.lastname: Tripathi
authors.affiliation: School of Biochemical Engineering, Indian Institute of Technology (Banaras Hindu University), Varanasi, 221005, India. pksrivastava.bce@itbhu.ac.in.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Srivastava
mesh_terms: D000818:Animals; D002356:Cartilage / Q000166:cytology / Q000502:physiology*; D002478:Cells, Cultured; D048271:Chitosan / Q000737:chemistry; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D016062:Porosity; D011817:Rabbits; D012038:Regeneration; D054457:Tissue Scaffolds
chemical_list: D011091:Polyesters; C033616:poly(lactide); D006820:Hyaluronic Acid; D048271:Chitosan
keywords: Airlift bioreactor; Biocompatibility; Cartilage; Chondrocyte; Scaffold