title: Construction of the Leaf Senescence Database and Functional Assessment of Senescence-Associated Genes.
abstract: Leaf senescence is the last phase of plant development and a highly coordinated process regulated by a large number of senescence-associated genes (SAGs). By broad literature survey, we constructed a leaf senescence database (LSD) in 2011 and updated it to Version 2.0 in 2014 ( http://www.eplantsenescence.org/ and http://psd.cbi.pku.edu.cn/ ) which contains a total of 5357 genes and 324 mutants from 44 species. These SAGs were retrieved based on genetic, genomic, proteomic, physiological, or other experimental evidence and were classified into different categories according to their functions in leaf senescence or morphological phenotype of mutants. To provide comprehensive information for SAGs, we made extensive annotation by both manual and computational approaches. In addition, we predicted putative orthologues of the SAGs in other species. LSD has a user-friendly interface to allow users to make text queries or BLAST searches and to download SAGs sequences for local analysis. Functional analyses of putative SAGs reveal that WRKY75, AZF2, NAC16, and WRKY26 are positive regulators of leaf senescence, while MKP2 and CTR1 perform negative regulation to leaf senescence. This database has been served as a valuable resource for basic research on the function of SAGs and evolution of plant leaf senescence, as well as for the exploration of genetic traits in agronomically important plants.
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences and Center for Bioinformatics, Peking University, Beijing, 100871, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.|Current Address: Department of Microbiology, Biochemistry, and Molecular Genetics, Rutgers University, New Brunswick, NJ, 07103, USA.
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences and Center for Bioinformatics, Peking University, Beijing, 100871, China.|Channing Division of Network Medicine, Brigham and Women's Hospital and Harvard Medical School, 181 Longwood Ave, Boston, MA, 02115, USA.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Peng
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences and Center for Bioinformatics, Peking University, Beijing, 100871, China.
authors.forename: Jinpu
authors.initials: J
authors.lastname: Jin
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing, 100871, China. hongweig@pku.edu.cn.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences and Center for Bioinformatics, Peking University, Beijing, 100871, China. luojc@pku.edu.cn.
authors.forename: Jingchu
authors.initials: J
authors.lastname: Luo
mesh_terms: D016922:Cellular Senescence / Q000235:genetics*; D019295:Computational Biology / Q000379:methods*; D030561:Databases, Nucleic Acid; D017343:Genes, Plant; D058977:Molecular Sequence Annotation; D009154:Mutation; D010641:Phenotype; D018515:Plant Leaves / Q000235:genetics*; D010944:Plants / Q000235:genetics*; D012984:Software; D064878:Web Browser
keywords: Database annotation; Leaf senescence; Orthologue; Senescence-associated gene