title: Bidirectional Anticipation of Future Osmotic Challenges by Vasopressin Neurons.
abstract: Ingestion of water and food are major hypo- and hyperosmotic challenges. To protect the body from osmotic stress, posterior pituitary-projecting, vasopressin-secreting neurons (VPppneurons) counter osmotic perturbations by altering their release of vasopressin, which controls renal water excretion. Vasopressin levels begin to fall within minutes of water consumption, even prior to changes in blood osmolality. To ascertain the precise temporal dynamics by which water or food ingestion affect VPppneuron activity, we directly recorded the spiking and calcium activity of genetically defined VPppneurons. In states of elevated osmolality, water availability rapidly decreased VPppneuron activity within seconds, beginning prior to water ingestion, upon presentation of water-predicting cues. In contrast, food availability following food restriction rapidly increased VPppneuron activity within seconds, but only following feeding onset. These rapid and distinct changes in activity during drinking and feeding suggest diverse neural mechanisms underlying anticipatory regulation of VPppneurons.
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Yael
authors.initials: Y
authors.lastname: Mandelblat-Cerf
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA; Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Angela
authors.initials: A
authors.lastname: Kim
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Christian R
authors.initials: CR
authors.lastname: Burgess
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.
authors.forename: Siva
authors.initials: S
authors.lastname: Subramanian
authors.affiliation: Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA; Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA 02129, USA.
authors.forename: Bakhos A
authors.initials: BA
authors.lastname: Tannous
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA; Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA. Electronic address: blowell@bidmc.harvard.edu.
authors.forename: Bradford B
authors.initials: BB
authors.lastname: Lowell
authors.affiliation: Division of Endocrinology, Diabetes, and Metabolism, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA; Program in Neuroscience, Harvard Medical School, Boston, MA 02115, USA. Electronic address: manderma@bidmc.harvard.edu.
authors.forename: Mark L
authors.initials: ML
authors.lastname: Andermann
mesh_terms: D000818:Animals; D001127:Arginine Vasopressin / Q000378:metabolism*; D003463:Cues; D004327:Drinking Behavior / Q000502:physiology*; D005247:Feeding Behavior / Q000502:physiology*; D051379:Mice; D009474:Neurons / Q000378:metabolism / Q000502:physiology*; D009994:Osmolar Concentration; D009997:Osmotic Pressure; D013495:Supraoptic Nucleus / Q000166:cytology; D014667:Vasopressins / Q000378:metabolism
chemical_list: D014667:Vasopressins; D001127:Arginine Vasopressin
keywords: Electrophysiology; Fiber Photometry; Food and water cues; Food restriction; Neuroendocrine neuron; Plasma osmolality; Posterior pituitary; Preingestive; Presystemic; Supraoptic nucleus; Vasopressin