title: Association analysis of IGF-I gene expression with growth and reproductive traits in Jinghai yellow chickens.
abstract: The insulin-like growth factor, IGF-I, plays an important role in the development of growth and reproductive traits. Single-strand conformation polymorphism analysis was used to detect and analyze polymorphisms and expression profiles of the IGF-I gene and its association with growth and reproductive traits of Jinghai yellow chickens. A point mutation g.295T>C was detected in the IGF-I gene with three genotypes CC, CT, and TT. The CT and TT genotypes were found to be significantly (P < 0.05) superior to the CC genotype in 8-, 10-, 12-, 14-, and 16-week-age weight of chickens (for growth traits) and in body weight at first egg-laying (for reproductive traits). Comparison of the expression level between males and females showed similarity in their expression curves, with females showing relatively higher IGF-I expression than males in all studied tissues. A similar IGF-I expression pattern was observed in the breast and leg muscles of both males and females, with the leg muscle showing relative higher IGF-I expression than the breast muscle. Our results indicate that, g.295T>C mutation in the IGF-I gene affects certain growth and reproductive traits, and it could be used to provide a theoretical basis as well as marker-assisted selection to upgrade the development of Jinghai yellow chickens in future.
authors.affiliation: College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, China.|Department of Animal Production, Faculty of Agricultural Technology and Fish Science, Al-Neelain University, Khartoum, Sudan.|Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, Jiangsu, China.
authors.forename: M A
authors.initials: MA
authors.lastname: Abdalhag
authors.affiliation: Department of Animal Production, Faculty of Agricultural Technology and Fish Science, Al-Neelain University, Khartoum, Sudan.|Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, Jiangsu, China.
authors.forename: T
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Animal Production, Faculty of Agricultural Technology and Fish Science, Al-Neelain University, Khartoum, Sudan.|Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, Jiangsu, China.
authors.forename: L
authors.initials: L
authors.lastname: Duan
authors.affiliation: Department of Animal Production, Faculty of Agricultural Technology and Fish Science, Al-Neelain University, Khartoum, Sudan.|Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, Jiangsu, China.
authors.forename: T
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Animal Production, Faculty of Agricultural Technology and Fish Science, Al-Neelain University, Khartoum, Sudan.|Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, Jiangsu, China.
authors.forename: G
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Animal Production, Faculty of Agricultural Technology and Fish Science, Al-Neelain University, Khartoum, Sudan jywang@yzu.edu.cn.|Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou, Jiangsu, China jywang@yzu.edu.cn.
authors.forename: J
authors.initials: J
authors.lastname: Wang
authors.affiliation: Jiangsu Jinghai Poultry Group Co., Ltd., Nantong, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics*; D002645:Chickens / Q000235:genetics / Q000254:growth & development*; D005260:Female; D056726:Genetic Association Studies; D005838:Genotype; D007334:Insulin-Like Growth Factor I / Q000235:genetics*; D010641:Phenotype; D018807:Polymorphism, Single-Stranded Conformational; D040641:Quantitative Trait Loci; D012098:Reproduction; D012641:Selection, Genetic
chemical_list: D030161:Avian Proteins; D007334:Insulin-Like Growth Factor I