title: Comparison of DNA extraction methods for meat analysis.
abstract: Preventing adulteration of meat and meat products with less desirable or objectionable meat species is important not only for economical, religious and health reasons, but also, it is important for fair trade practices, therefore, several methods for identification of meat and meat products have been developed. In the present study, ten different DNA extraction methods, including Tris-EDTA Method, a modified Cetyltrimethylammonium Bromide (CTAB) Method, Alkaline Method, Urea Method, Salt Method, Guanidinium Isothiocyanate (GuSCN) Method, Wizard Method, Qiagen Method, Zymogen Method and Genespin Method were examined to determine their relative effectiveness for extracting DNA from meat samples. The results show that the salt method is easy to perform, inexpensive and environmentally friendly. Additionally, it has the highest yield among all the isolation methods tested. We suggest this method as an alternative method for DNA isolation from meat and meat products.
authors.affiliation: Bioanalysis Laboratory, TUBITAK National Metrology Institute (UME), Gebze, Kocaeli, Turkey; Department of Molecular Medicine, Institute for Experimental Medical Research, Istanbul University, Istanbul, Turkey. Electronic address: burhanettin.yalcinkaya@tubitak.gov.tr.
authors.forename: Burhanettin
authors.initials: B
authors.lastname: Yalçınkaya
authors.affiliation: Bioanalysis Laboratory, TUBITAK National Metrology Institute (UME), Gebze, Kocaeli, Turkey; Department of Molecular Biology and Genetics, Atatürk University, Erzurum, Turkey.
authors.forename: Eylem
authors.initials: E
authors.lastname: Yumbul
authors.affiliation: Bioanalysis Laboratory, TUBITAK National Metrology Institute (UME), Gebze, Kocaeli, Turkey.
authors.forename: Erkan
authors.initials: E
authors.lastname: Mozioğlu
authors.affiliation: Bioanalysis Laboratory, TUBITAK National Metrology Institute (UME), Gebze, Kocaeli, Turkey.
authors.forename: Muslum
authors.initials: M
authors.lastname: Akgoz
mesh_terms: D002593:Cetrimonium Compounds / Q000737:chemistry; D004247:DNA / Q000032:analysis / Q000302:isolation & purification*; D008460:Meat / Q000032:analysis*; D008461:Meat Products / Q000032:analysis*; D016133:Polymerase Chain Reaction; D013050:Spectrometry, Fluorescence
chemical_list: D002593:Cetrimonium Compounds; D004247:DNA
keywords: Adulteration; Biometrology; Fluorometric analysis; Genomic DNA extraction; Meat and meat products; Spectrophotometric analysis; qPCR