title: Electrodialytic separation of levulinic acid catalytically synthesized from woody biomass for use in microbial conversion.
abstract: Levulinic acid (LA) is produced by the catalytic conversion of a variety of woody biomass. To investigate the potential use of desalting electrodialysis (ED) for LA purification, electrodialytic separation of levulinate from both reagent and cedar-derived LA solution (40-160 g L-1) was demonstrated. When using reagent LA solution with pH5.0-6.0, the recovery rates of levulinate ranged from 68 to 99%, and the energy consumption for recovery of 1 kg of levulinate ranged from 0.18 to 0.27 kWh kg-1. With cedar-derived LA solution (pH6.0), good agreement in levulinate recovery (88-99%), and energy consumption (0.18-0.22 kWh kg-1) were observed in comparison to the reagent LA solutions, although a longer operation time was required due to some impurities. The application of desalting ED was favorable for promoting microbial utilization of cedar-derived LA. From 0.5 mol L-1of the ED-concentrated sodium levulinate solution, 95.6% of levulinate was recovered as LA calcium salt dihydrate by crystallization. This is the first report on ED application for LA recovery using more than 20 g L-1LA solutions (40-160 g L-1). © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:448-453, 2017.
authors.affiliation: Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.|Research Institute for Sustainable Chemistry, AIST, Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Habe
authors.affiliation: Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.
authors.forename: Susumu
authors.initials: S
authors.lastname: Kondo
authors.affiliation: Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.
authors.forename: Yuya
authors.initials: Y
authors.lastname: Sato
authors.affiliation: Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 16-1 Onogawa, Tsukuba, Ibaraki, 305-8569, Japan.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Hori
authors.affiliation: Bioproduction Research Institute, AIST, Tsukuba Central 6-10, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Kanno
authors.affiliation: Bioproduction Research Institute, AIST, Tsukuba Central 6-10, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
authors.forename: Nobutada
authors.initials: N
authors.lastname: Kimura
authors.affiliation: Bioproduction Research Institute, AIST, Tsukuba Central 6-10, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Koike
authors.affiliation: Dept. of Applied Chemistry, Faculty of Science and Engineering, Waseda University, Tokyo, 169-8555, Japan.
authors.forename: Kohtaro
authors.initials: K
authors.lastname: Kirimura
mesh_terms: D018533:Biomass; D002384:Catalysis; D002482:Cellulose / Q000737:chemistry*; D004586:Electrophoresis / Q000379:methods*; D007982:Levulinic Acids / Q000737:chemistry / Q000302:isolation & purification* / Q000378:metabolism*; D011549:Pseudomonas / Q000378:metabolism*; D014934:Wood / Q000737:chemistry* / Q000382:microbiology*
chemical_list: D007982:Levulinic Acids; D002482:Cellulose; C032246:levulinic acid
keywords: Levulinate; Pseudomonas sp; cellulosic biomass; desalting electrodialysis; microbial conversion