title: Nanoparticle passage through porcine jejunal mucus: Microfluidics and rheology.
abstract: A micro-slide chamber was used to screen and rank sixteen functionalized fluorescent silica nanoparticles (SiNP) of different sizes (10, 50, 100 and 200 nm) and surface coatings (aminated, carboxylated, methyl-PEG1000ylated, and methyl-PEG2000ylated) according to their capacity to permeate porcine jejunal mucus. Variables investigated were influence of particle size, surface charge and methyl-PEGylation. The anionic SiNP showed higher transport through mucus whereas the cationic SiNP exhibited higher binding with lower transport. A size-dependence in transport was identified - 10 and 50 nm anionic (uncoated or methyl-PEGylated) SiNP showed higher transport compared to the larger 100 and 200 nm SiNP. The cationic SiNP of all sizes interacted with the mucus, making it more viscous and less capable of swelling. In contrast, the anionic SiNP (uncoated or methyl-PEGylated) caused minimal changes in the viscoelasticity of mucus. The data provide insights into mucus-NP interactions and suggest a rationale for designing oral nanomedicines with improved mucopermeability.
authors.affiliation: Conway Institute of Biomolecular and Biomedical Research, University College Dublin (UCD), Belfield, Dublin 4, Ireland; School of Veterinary Medicine, University College Dublin (UCD), Belfield, Dublin 4, Ireland. Electronic address: sourav.bhattacharjee@ucd.ie.
authors.forename: Sourav
authors.initials: S
authors.lastname: Bhattacharjee
authors.affiliation: Conway Institute of Biomolecular and Biomedical Research, University College Dublin (UCD), Belfield, Dublin 4, Ireland.
authors.forename: Eugene
authors.initials: E
authors.lastname: Mahon
authors.affiliation: School of Agriculture and Food Science, University College Dublin (UCD), Belfield, Dublin 4, Ireland.
authors.forename: Sabine M
authors.initials: SM
authors.lastname: Harrison
authors.affiliation: School of Veterinary Medicine, University College Dublin (UCD), Belfield, Dublin 4, Ireland.
authors.forename: Jim
authors.initials: J
authors.lastname: McGetrick
authors.affiliation: National Institute for Bioprocessing Research and Training (NIBRT), University College Dublin (UCD), Belfield, Dublin 4, Ireland.
authors.forename: Mohankumar
authors.initials: M
authors.lastname: Muniyappa
authors.affiliation: School of Veterinary Medicine, University College Dublin (UCD), Belfield, Dublin 4, Ireland.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Carrington
authors.affiliation: Conway Institute of Biomolecular and Biomedical Research, University College Dublin (UCD), Belfield, Dublin 4, Ireland; School of Veterinary Medicine, University College Dublin (UCD), Belfield, Dublin 4, Ireland.
authors.forename: David J
authors.initials: DJ
authors.lastname: Brayden
mesh_terms: D000818:Animals; D001692:Biological Transport; D004337:Drug Carriers / Q000032:analysis / Q000493:pharmacokinetics; D004867:Equipment Design; D007583:Jejunum / Q000378:metabolism*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D009093:Mucus / Q000378:metabolism*; D053758:Nanoparticles / Q000032:analysis* / Q000648:ultrastructure; D011092:Polyethylene Glycols / Q000032:analysis / Q000493:pharmacokinetics; D012212:Rheology; D012822:Silicon Dioxide / Q000032:analysis* / Q000493:pharmacokinetics*; D013499:Surface Properties; D013552:Swine; D014783:Viscosity
chemical_list: D004337:Drug Carriers; D011092:Polyethylene Glycols; D012822:Silicon Dioxide
keywords: ImageJ®; Mucodiffusion; Mucus rheology; Nano-DDS; Oral drug delivery; Silica nanoparticles