title: In vitro Vasorelaxant Effect of Artemisia herba alba Asso. in Spontaneously Hypertensive Rats.
abstract: BACKGROUND
  The present study aims to evaluate the in vitro vasorelaxant effect of Artemisia herba alba (Ah) aqueous extract.
  
  
  METHODS
  Aortic rings were isolated from spontaneously hypertensive rats and incubated in aqueous Ah extract at the following doses: 3, 5, 10 and 20 mg/ml. Incubation of aqueous Ah extract for 30 minutes produced a significant shift of the dose response curve to Norepinephrine (NE) (10-8 to 10-5 M) (p<0.001).
  
  
  RESULTS
  Endothelium ablation significantly reduced the vasorelaxant effect of aqueous Ah extract (p<0.001). In addition, inhibition of vascular nitric oxide by Nω-Nitro-L-Arginine Methyl (LNAME) produced a significant reduction in the vasorelaxant effect of aqueous Ah extract (p<0.001).
  
  
  CONCLUSIONS
  We conclude that aqueous Ah extract at a dose of 20 mg/ml possess an in vitro vasorelaxant effect which seems to be dependent on the endothelium vasorelaxant factors involving nitric oxide synthesis.
authors.affiliation: High School of Nursing and Health Techniques of Rabat, Avenue Hassan II Km 4,5 Rabat, Morocco.
authors.forename: Zeggwagh
authors.initials: Z
authors.lastname: Naoufel
authors.affiliation: Laboratory of Physiology and Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, B.P. 21, Errachidia, 52000, Morocco.
authors.forename: Morad
authors.initials: M
authors.lastname: Hebi
authors.affiliation: Laboratory of Physiology and Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, B.P. 21, Errachidia, 52000, Morocco.
authors.forename: Mohammed
authors.initials: M
authors.lastname: Ajebli
authors.affiliation: U1148 INSERM, Faculty of Medicine Xavier Bichat, Paris, France.
authors.forename: Jean B
authors.initials: JB
authors.lastname: Michel
authors.affiliation: Laboratory of Physiology and Endocrine Pharmacology, Faculty of Sciences and Techniques Errachidia, Moulay Ismail University, BP 21, Errachidia, 52000, Morocco.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Eddouks
mesh_terms: D000818:Animals; D019306:Artemisia / Q000737:chemistry*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004730:Endothelium, Vascular / Q000166:cytology / Q000187:drug effects*; D006973:Hypertension / Q000188:drug therapy; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D011918:Rats, Inbred SHR; D014665:Vasodilator Agents / Q000494:pharmacology*
chemical_list: D010936:Plant Extracts; D014665:Vasodilator Agents
keywords: Aortic rings; Artemisia herba alba; endothelium vasorelaxant factors; ethnophamacology; hypertension; nitric oxide
  synthesis