title: Dietary live yeast and mannan-oligosaccharide supplementation attenuate intestinal inflammation and barrier dysfunction induced by Escherichia coli in broilers.
abstract: The effects of live yeast (LY) and mannan-oligosaccharide (MOS) supplementation on intestinal disruption induced by Escherichia coli in broilers were investigated. The experimental design was a 3×2 factorial arrangement with three dietary treatments (control, 0·5 g/kg LY (Saccharomyces cerevisiae, 1·0×1010 colony-forming units/g), 0·5 g/kg MOS) and two immune treatments (with or without E. coli challenge from 7 to 11 d of age). Samples were collected at 14 d of age. The results showed that E. coli challenge impaired (P<0·05) growth performance during the grower period (1-21 d) and the overall period (1-35 d) of broilers, increased (P<0·05) serum endotoxin and diamine oxidase levels coupled with ileal myeloperoxidase and lysozyme activities, whereas reduced (P<0·05) maltase activity, and compromised the morphological structure of the ileum. Besides, it increased (P<0·05) the mRNA expressions of several inflammatory genes and reduced occludin expression in the ileum. Dietary treatment with both LY and MOS reduced (P<0·05) serum diamine oxidase and ileal myeloperoxidase levels, but elevated villus height (P<0·10) and the ratio of villus height:crypt depth (P<0·05) of the ileum. It also alleviated (P<0·05) E. coli-induced increases (P<0·05) in ileal Toll-like receptor 4, NF-κ B and IL-1 β expressions. Moreover, LY supplementation reduced (P<0·05) feed conversion ratio of birds during the grower period and enhanced (P<0·05) the community diversity (Shannon and Simpson indices) of ileal microbiota, whereas MOS addition counteracted (P<0·05) the decreased ileal IL-10 and occludin expressions in challenged birds. In conclusion, both LY and MOS supplementation could attenuate E. coli-induced intestinal disruption by alleviating intestinal inflammation and barrier dysfunction in broilers. Moreover, LY addition could improve intestinal microbial community structure and feed efficiency of broilers.
authors.affiliation: 1State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing,100193,People's Republic of China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing,100193,People's Republic of China.
authors.forename: Zhui
authors.initials: Z
authors.lastname: Li
authors.affiliation: 1State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing,100193,People's Republic of China.
authors.forename: Qiqi
authors.initials: Q
authors.lastname: Han
authors.affiliation: 1State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing,100193,People's Republic of China.
authors.forename: Yuming
authors.initials: Y
authors.lastname: Guo
authors.affiliation: 2Phileo Lesaffre Animal Care (China),Beijing,100027,People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: 3Phileo Lesaffre Animal Care,Lille,59700,France.
authors.forename: Romain
authors.initials: R
authors.lastname: D'inca
mesh_terms: D000818:Animals; D017849:Animals, Inbred Strains; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002645:Chickens; D002681:China; D002149:Energy Intake; D004751:Enteritis / Q000178:diet therapy / Q000209:etiology / Q000378:metabolism / Q000662:veterinary*; D004927:Escherichia coli Infections / Q000276:immunology / Q000382:microbiology / Q000503:physiopathology / Q000662:veterinary*; D000069196:Gastrointestinal Microbiome; D018507:Gene Expression Regulation, Developmental; D007082:Ileum / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D007413:Intestinal Mucosa / Q000378:metabolism / Q000382:microbiology / Q000473:pathology / Q000503:physiopathology; D008297:Male; D008351:Mannans / Q000627:therapeutic use*; D062793:Occludin / Q000235:genetics / Q000378:metabolism; D011201:Poultry Diseases / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D056692:Prebiotics; D019936:Probiotics / Q000627:therapeutic use*; D011897:Random Allocation; D012441:Saccharomyces cerevisiae / Q000254:growth & development / Q000502:physiology*; D015430:Weight Gain
chemical_list: D030161:Avian Proteins; D015415:Biomarkers; D008351:Mannans; D062793:Occludin; D056692:Prebiotics
keywords:  ABW average body weight;  ADFI average daily feed intake;  ADG average daily gain;  DAO diamine oxidase;  FCR feed conversion ratio;  LY live yeast;  MOS mannan-oligosaccharide;  MPO myeloperoxidase;  TJ tight junctions;  TLR Toll-like receptor;  VCR villus height:crypt depth ratio; Broilers; Intestinal barrier dysfunction; Intestinal inflammation; Live yeast; Mannan-oligosaccharide