title: Tau Oligomers as Pathogenic Seeds: Preparation and Propagation In Vitro and In Vivo.
abstract: Tau oligomers have been shown to be the main toxic tau species in a number of neurodegenerative disorders. In order to study tau oligomers both in vitro and in vivo, we have established methods for the reliable preparation, isolation, and detection of tau oligomers. Methods for the seeding of tau oligomers, isolation of tau oligomers from tissue, and detection of tau oligomers using tau oligomer-specific antibodies by biochemical and immunohistochemical methods are detailed below.
authors.affiliation: George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA.|Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555-1045, USA.
authors.forename: Julia E
authors.initials: JE
authors.lastname: Gerson
authors.affiliation: George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA.|Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555-1045, USA.
authors.forename: Urmi
authors.initials: U
authors.lastname: Sengupta
authors.affiliation: George P. and Cynthia Woods Mitchell Center for Neurodegenerative Diseases, University of Texas Medical Branch, Galveston, TX, 77555, USA. rakayed@utmb.edu.|Departments of Neurology, and Neuroscience and Cell Biology, University of Texas Medical Branch, Galveston, TX, 77555, USA. rakayed@utmb.edu.|University of Texas Medical Branch, 301 University Boulevard, Medical Research Building, Room 10.138C, Galveston, TX, 77555-1045, USA. rakayed@utmb.edu.
authors.forename: Rakez
authors.initials: R
authors.lastname: Kayed
mesh_terms: D000682:Amyloid / Q000378:metabolism; D000818:Animals; D006801:Humans; D007150:Immunohistochemistry; D024801:Tauopathies / Q000378:metabolism*; D016875:tau Proteins / Q000737:chemistry* / Q000302:isolation & purification*
chemical_list: D000682:Amyloid; D016875:tau Proteins
keywords: Amyloid seeding; Tau oligomer isolation; Tau oligomers; Tauopathies