title: The cell wall DUF642 At2g41800 (TEB) protein is involved in hypocotyl cell elongation.
abstract: In plants, the cell wall is a complex and dynamic structure comprising high molecular weight carbohydrates and proteins. The cell wall plays an important role in several stages of the plant life cycle, including cell division, elongation and differentiation. The DUF642 family of cell wall proteins is highly conserved in spermatophytes and might be involved in pectin structural modifications. Particularly, At2g41800 is one of the most highly induced genes during the M/G1 phases of the cell cycle, and the protein encodes by this gene has been detected in cell wall proteomes of cell suspension cultures. In the present study, the expression of At2g41800 (TEB) was confirmed in primary and lateral roots, stigmatic papillae and hypocotyls. Subcellular localization studies showed that TEB is located in the cell wall. The root length and lateral root density were not affected in either of the two teb mutants studied, but the length of the hypocotyls from seedlings grown under light and dark conditions was increased. Immunogold labelling studies using JIM5 antibodies on sections of hypocotyl epidermal cells showed an important reduction of gold particles in teb mutants. The results suggested that TEB is involved in hypocotyl elongation.
authors.affiliation: Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP. 04510, México DF, Mexico.
authors.forename: Alexis
authors.initials: A
authors.lastname: Salazar-Iribe
authors.affiliation: Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP. 04510, México DF, Mexico.
authors.forename: Lourdes Teresa
authors.initials: LT
authors.lastname: Agredano-Moreno
authors.affiliation: Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP. 04510, México DF, Mexico.
authors.forename: Esther
authors.initials: E
authors.lastname: Zúñiga-Sánchez
authors.affiliation: Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP. 04510, México DF, Mexico.
authors.forename: Luis Felipe
authors.initials: LF
authors.lastname: Jiménez-Garcia
authors.affiliation: Instituto de Ecología, Universidad Nacional Autónoma de México, Ciudad Universitaria, CP. 04510, México DF, Mexico. Electronic address: agamboa@ecologia.unam.mx.
authors.forename: Alicia
authors.initials: A
authors.lastname: Gamboa-deBuen
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D049108:Cell Enlargement; D002473:Cell Wall / Q000378:metabolism*; D018546:Hypocotyl / Q000254:growth & development*
chemical_list: D029681:Arabidopsis Proteins; C000618212:TEB DUF642 family protein, Arabidopsis
keywords: Arabidopsis thaliana; Cell wall; DUF642 proteins; Hypocotyls; Pectin