title: JSI-124 inhibits IgE production in an IgE B cell line.
abstract: IgE is a key effector molecule in atopic diseases; however, the regulation mechanisms of IgE production in IgE B cells remain poorly understood. In the present study, we demonstrate that JSI-124 (cucurbitacin I), a selective STAT3 inhibitor, selectively inhibits production of IgE by a human IgE B cell line, CRL-8033 cells, while does not affect the IgG production by IgG B cell lines. In the aspect of molecular mechanism, we found that Igλ, but not Ighe, gene expression was suppressed by JSI-124. The above effects of JSI-124 were not mediated by affecting cellular proliferation or apoptosis. Furthermore, multiple B cell differentiation-related genes expression was not significantly affected by JSI-124. Taken together, we demonstrate a potential strategy of therapeutically suppressing IgE production without affecting IgG production in atopic patients.
authors.affiliation: Division of Immunology, School of Fundamental Medicine, Jinzhou Medical University, Jinzhou, 121000, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Cui
authors.affiliation: Shenzhen Laboratory of Antibody Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Bi
authors.affiliation: Shenzhen Laboratory of Antibody Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China.
authors.forename: Dehong
authors.initials: D
authors.lastname: Yan
authors.affiliation: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, China.
authors.forename: Xiufeng
authors.initials: X
authors.lastname: Ye
authors.affiliation: Shenzhen Second People's Hospital, The First Affiliated Hospital of Shenzhen University, Shenzhen, 518035, China.
authors.forename: Mingxing
authors.initials: M
authors.lastname: Zheng
authors.affiliation: Division of Immunology, School of Fundamental Medicine, Jinzhou Medical University, Jinzhou, 121000, China. Electronic address: ly65401@sina.cn.
authors.forename: Guang
authors.initials: G
authors.lastname: Yu
authors.affiliation: Shenzhen Laboratory of Antibody Engineering, Institute of Biomedicine and Biotechnology, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China. Electronic address: xc.wan@siat.ac.cn.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wan
mesh_terms: D017209:Apoptosis / Q000187:drug effects; D001402:B-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D049109:Cell Proliferation; D015870:Gene Expression / Q000187:drug effects; D005803:Genes, Immunoglobulin / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000096:biosynthesis* / Q000235:genetics; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000235:genetics; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: D007074:Immunoglobulin G; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D014315:Triterpenes; D007073:Immunoglobulin E; C038106:cucurbitacin I
keywords: Atopic diseases; IgE; JSI-124