title: Glomerular loss after arteriovenous and arterial clamping for renal warm ischemia in a swine model.
abstract: To evaluate the glomerular loss after arteriovenous or arterial warm ischemia in a swine model.
  Twenty four pigs were divided into Group Sham (submitted to all surgical steps except the renal ischemia), Group AV (submitted to 30 minutes of warm ischemia by arteriovenous clamping of left kidney vessels), and Group A (submitted to 30 minutes of ischemia by arterial clamping). Right kidneys were used as controls. Weigh, volume, cortical volume, glomerular volumetric density (Vv[Glom]), volume-weighted glomerular volume (VWGV), and the total number of glomeruli were measured for each organ.
  Group AV showed a 24.5% reduction in its left kidney Vv[Glom] and a 25.4% reduction in the VWGV, when compared to the right kidney. Reductions were also observed when compared to kidneys of sham group. There was a reduction of 19.2% in the total number of glomeruli in AV kidneys. No difference was observed in any parameters analyzed on the left kidneys from group A.
  Renal warm ischemia of 30 minutes by arterial clamping did not caused significant glomerular damage, but arteriovenous clamping caused significant glomerular loss in a swine model. Clamping only the renal artery should be considered to minimize renal injury after partial nephrectomies.
authors.affiliation: Master, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, Universidade Estadual do Rio de Janeiro (UERJ), Brazil. Conception and design of the study, acquisition and interpretation of data, final approval of the manuscript.
authors.forename: Gustavo Ruschi
authors.initials: GR
authors.lastname: Bechara
authors.affiliation: Master, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, UERJ, Rio de Janeiro-RJ. Assistant Professor, Department of Veterinary Clinical Pathology, Universidade Federal Fluminense (UFF), Niteroi-RJ, Brazil. Acquisition and interpretation of data, manuscript preparation, final approval of the manuscript.
authors.forename: José Aurelino
authors.initials: JA
authors.lastname: Damasceno-Ferreira
authors.affiliation: Master, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, UERJ. Assistant Professor, Faculty of Medicine, Universidade Estácio de Sá, Rio de Janeiro-RJ, Brazil. Acquisition and interpretation of data, manuscript preparation, final approval of the manuscript.
authors.forename: Leonardo Albuquerque Dos Santos
authors.initials: LA
authors.lastname: Abreu
authors.affiliation: PhD, Visiting Researcher, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, UERJ, Rio de Janeiro-RJ, Brazil. Conception and design of the study, acquisition and interpretation of data, final approval of the manuscript.
authors.forename: Waldemar Silva
authors.initials: WS
authors.lastname: Costa
authors.affiliation: PhD, Full Professor, Head, Postgraduate Program in Physiopathology and Surgical Sciences. Head, Urogenital Research Unit, UERJ, Rio de Janeiro-RJ, Brazil. Conception and design of the study, acquisition and interpretation of data, final approval of the manuscript.
authors.forename: Francisco José Barcellos
authors.initials: FJ
authors.lastname: Sampaio
authors.affiliation: PhD, Associate Professor, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, UERJ, Rio de Janeiro-RJ. Associate Professor, Department of Morphology, UFF, Niteroi-RJ, Brazil. Conception and design of the study, acquisition and interpretation of data, manuscript preparation, final approval of the manuscript.
authors.forename: Marco Aurélio
authors.initials: MA
authors.lastname: Pereira-Sampaio
authors.affiliation: PhD, Associate Professor, Postgraduate Program in Physiopathology and Surgical Sciences, Urogenital Research Unit, UERJ, Rio de Janeiro-RJ, Brazil. Conception and design of the study, acquisition and interpretation of data, manuscript preparation, final approval of the manuscript.
authors.forename: Diogo Benchimol De
authors.initials: DB
authors.lastname: Souza
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D007678:Kidney Glomerulus / Q000033:anatomy & histology / Q000098:blood supply* / Q000503:physiopathology; D010535:Laparoscopy / Q000379:methods*; D008297:Male; D009392:Nephrectomy / Q000379:methods*; D009929:Organ Size; D012077:Renal Artery / Q000601:surgery*; D013552:Swine; D052096:Warm Ischemia / Q000379:methods*