title: The effects of pprI gene of Deinococcus radiodurans R1 on acute radiation injury of mice exposed to 60Co γ-ray radiation.
abstract: The role of the pprI gene from Deinococcus radiodurans R1 in therapy of acute radiation injury of a mammalian host was investigated. We injected a plasmid containing the pprI gene into the muscle of mice exposed to total 6Gy of 60Co γ-ray radiation. After injection, we used in vivo gene electroporation technology to transfer the pprI gene into the cell. We found the PprI protein was expressed significantly at 1 d after irradiation, but there was no expression of pprI gene 7 d post-irradiation. The expression of pprI gene evidently decreased the death rate of mice exposed to lethal dose radiation, significantly relieved effects on blood cells in the acute stage, shortened the persistence time of the decrease of lymphocytes, and decreased the apoptotic rates of spleen cells, thymocytes and bone marrow cells. The expression of Rad51 protein in the lungs, livers, and kidneys was significantly higher in the mice treated with the pprI plasmid after irradiation. However, there were no obvious differences for Rad52 protein expression. We conclude that the prokaryotic pprI gene of D. radiodurans R1 first was expressed in mammalian cells. The expressed prokaryotic PprI protein has distinct effects of the prevention and treatment on acute radiation injury of mammal. The effects of radio-resistance may relate to expression of Rad51 protein which is homologous with RecA from D. radiodurans.
authors.affiliation: Department of Oncology, The People`s Hospital of Subei, Yangzhou, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Chen
authors.affiliation: Department of Oncology, The People`s Hospital of Subei, Yangzhou, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hua
authors.affiliation: Department of Oncology, The People`s Hospital of Subei, Yangzhou, China.
authors.forename: Xi-Zhi
authors.initials: XZ
authors.lastname: Zhang
authors.affiliation: Department of Oncology, The People`s Hospital of Subei, Yangzhou, China.
authors.forename: Bu-Hai
authors.initials: BH
authors.lastname: Wang
authors.affiliation: School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou, China.
authors.forename: Zhan-Shan
authors.initials: ZS
authors.lastname: Yang
mesh_terms: D000208:Acute Disease; D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D004249:DNA Damage / Q000235:genetics; D004260:DNA Repair / Q000235:genetics*; D034301:Deinococcus / Q000235:genetics*; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D005798:Genes, Bacterial; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D011832:Radiation Injuries / Q000235:genetics* / Q000401:mortality / Q000517:prevention & control; D011836:Radiation Tolerance / Q000235:genetics*
chemical_list: D001426:Bacterial Proteins
keywords: Pathology Section; bacteria; gene therapy; in vivo gene electroporation; pprI gene; radiation protection