title: Gastroprotective effects of Kangfuxin-against ethanol-induced gastric ulcer via attenuating oxidative stress and ER stress in mice.
abstract: Oxidative stress and ER stress play a role in the pathogenesis of gastric ulcer. Kangfuxin (KFX) has been used to treat gastric ulcer in patients. However, the underlying mechanisms of KFX action remain unclear. The current study was undertaken to evaluate the gastroprotective effects of KFX and to determine its potential mechanisms. Ethanol-induced gastric ulcer mouse model was employed. Ethanol pretreated mice were treated with low (0.02 g/kg) and high (0.05 g/kg) dose of KFX for 14 days. Cimetidine (0.8 g/kg) was used as positive control. Histological evaluation of the gastric mucosa revealed that mice treated with ethanol exhibited severe gastric mucosal damage. Ethanol treatment increased plasma and gastric MDA level, decreased plasma and gastric SOD activity, and reduced gastric HO-1 and GCL-c mRNA levels. ER stress markers (CHOP, GRP78, and caspase 12) were up-regulated upon ethanol administration. Moreover, increased cell apoptosis and pro-apoptotic protein Bax and caspase 3 were observed in ethanol treated mice, while the anti-apoptotic protein Bcl 2 was inhibited. Finally, KFX treatment reversed ethanol-induced phenotypes and ameliorated gastric ulcer. Our results demonstrated that the gastroprotective effects of KFX against ethanol-induced gastric ulcer could be attributed to its anti-oxidative stress, anti-ER stress and anti-apoptotic effects.
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Pianpian
authors.initials: P
authors.lastname: Chen
authors.affiliation: Sichuan Key Laboratory of Medical American Cockroach, Chengdu, 610000, China.
authors.forename: Yongmei
authors.initials: Y
authors.lastname: Shen
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Hongxue
authors.initials: H
authors.lastname: Shi
authors.affiliation: Sichuan Key Laboratory of Medical American Cockroach, Chengdu, 610000, China.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Ma
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Lin
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Xiao
authors.affiliation: Science and Education Division, Cixi People's Hospital, Wenzhou Medical University, Ningbo, 315300, China.
authors.forename: Fenzan
authors.initials: F
authors.lastname: Wu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Zhengmao
authors.initials: Z
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiao
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Sciences, Key Laboratory of Biotechnology and Pharmaceutical Engineering, Wenzhou Medical University, Wenzhou, 325035, China. Electronic address: st_hyz@126.com.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Sichuan Key Laboratory of Medical American Cockroach, Chengdu, 610000, China. Electronic address: haoyishenggfn@126.com.
authors.forename: Funeng
authors.initials: F
authors.lastname: Geng
keywords: Apoptosis; ER stress; Gastroprotection; Kangfuxin; Oxidative stress