title: Gas Purge Microextraction Coupled with Stable Isotope Labeling-Liquid Chromatography/Mass Spectrometry for the Analysis of Bromophenols in Aquatic Products.
abstract: A green, sensitive, and accurate method was developed for the extraction and determination of bromophenols (BPs) from aquatic products by using organic solvent-free gas purge microsyringe extraction (GP-MSE) technique in combination with stable isotope labeling (SIL) strategy. BPs were extracted by NaHCO3buffer solution, with recoveries varying from 92.0% to 98.5%. The extracted solution was analyzed by SIL strategy, during which analytes and standards were labeled by 10-methyl-acridone-2-sulfonyl chloride (d0-MASC) and its deuterated counterpart d3-MASC, respectively. The labeling reaction was finished within 10 min with good stability. The liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) sensitivity of BPs was greatly enhanced due to the mass-enhancing property of MASC, while the matrix effect was effectively minimized by the SIL strategy. The limits of detection (LODs) were in the range of 0.10-0.30 μg/kg, while the limits of quantitations (LOQs) were in the range of 0.32-1.0 μg/kg. The proposed method also showed great potential in the qualitative analysis of other bromophenols in the absence of standard.
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University , West Jingxuan Road no. 57, Qufu 273165, PR China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, PR China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University , West Jingxuan Road no. 57, Qufu 273165, PR China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, PR China.
authors.forename: Qiuhui
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University , West Jingxuan Road no. 57, Qufu 273165, PR China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, PR China.
authors.forename: Cuncun
authors.initials: C
authors.lastname: Sheng
authors.affiliation: Shandong Province Key Laboratory of Life-Organic Analysis, Qufu Normal University , West Jingxuan Road no. 57, Qufu 273165, PR China.|Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, Qufu Normal University , Qufu 273165, PR China.|Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Science , Xining 810008, PR China.
authors.forename: Jinmao
authors.initials: J
authors.lastname: You
mesh_terms: D000818:Animals; D005591:Chemical Fractionation / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003903:Deuterium / Q000737:chemistry; D005399:Fishes; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D033541:Palaemonidae / Q000737:chemistry; D010636:Phenols / Q000032:analysis* / Q000302:isolation & purification; D017747:Seafood / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010636:Phenols; C038964:2,6-dibromophenol; C032038:2-bromophenol; D003903:Deuterium; C032037:4-bromophenol
keywords: GP−MSE; aquatic products; bromophenols; stable isotope labeling