title: Characterization of an Immunodominant Epitope in the Endodomain of the Coronavirus Membrane Protein.
abstract: The coronavirus membrane (M) protein acts as a dominant immunogen and is a major player in virus assembly. In this study, we prepared two monoclonal antibodies (mAbs; 1C3 and 4C7) directed against the transmissible gastroenteritis virus (TGEV) M protein. The 1C3 and 4C7 mAbs both reacted with the native TGEV M protein in western blotting and immunofluorescence (IFA) assays. Two linear epitopes, 243YSTEART249 (1C3) and 243YSTEARTDNLSEQEKLLHMV262 (4C7), were identified in the endodomain of the TGEV M protein. The 1C3 mAb can be used for the detection of the TGEV M protein in different assays. An IFA method for the detection of TGEV M protein was optimized using mAb 1C3. Furthermore, the ability of the epitope identified in this study to stimulate antibody production was also evaluated. An immunodominant epitope in the TGEV membrane protein endodomain was identified. The results of this study have implications for further research on TGEV replication.
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. donghui_12215084@126.com.|Molecular Biology (Gembloux Agro-Bio Tech), University of Liège (ULg), Liège 4000, Belgium. donghui_12215084@126.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. zhangxin2410@163.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. shy2005y@163.com.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. chenjianfei@126.com.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. dashi198566@163.com.
authors.forename: Da
authors.initials: D
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. zyn18345038162@163.com.
authors.forename: Yunnuan
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Veterinary Biotechnology, Division of Swine Infectious Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China. fl@hvri.ac.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Feng
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology / Q000302:isolation & purification; D000914:Antibodies, Viral / Q000276:immunology / Q000302:isolation & purification; D015153:Blotting, Western; D002460:Cell Line; D000086862:Coronavirus M Proteins; D018604:Epitope Mapping; D005455:Fluorescent Antibody Technique; D016056:Immunodominant Epitopes / Q000276:immunology*; D008807:Mice, Inbred BALB C; D013552:Swine; D005760:Transmissible gastroenteritis virus / Q000276:immunology*; D014763:Viral Matrix Proteins / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000914:Antibodies, Viral; D000086862:Coronavirus M Proteins; D016056:Immunodominant Epitopes; C000706238:M protein, Transmissible gastroenteritis virus; D014763:Viral Matrix Proteins
keywords: coronavirus; endodomain; immunodominant epitope; membrane protein