title: Enhanced Expression of Trib3 during the Development of Myelin Breakdown in dmy Myelin Mutant Rats.
abstract: The demyelination (dmy) rat exhibits hind limb ataxia and severe myelin breakdown in the central nervous system. The causative gene of dmy rats is the MRS2 magnesium transporter gene. Tribbles homolog 3 (Trib3) is a pseudokinase molecule that modifies certain signal pathways, and its expression is increased in response to various stresses. Here we sought to clarify the mechanism of myelin breakdown by focusing Trib3, which is remarkably up-regulated in dmy rats. The expression of Trib3 mRNA was significantly increased at 4, 5, 6, 7 and 8 weeks of age in the dmy rats, prior to the prominent myelin breakdown between 7 and 10 weeks of age. The expression level of Trib3 was increased concurrently with the progression of the clinical and pathological conditions in the dmy rats. Double immunofluorescence demonstrated that TRIB3 was mainly expressed in neurons and oligodendrocytes and localized in the Golgi apparatus. Our findings indicate that Trib3 may be associated with the pathogenic mechanism of dmy rats.
authors.affiliation: Laboratory of Veterinary Pathology, Osaka Prefecture University, Izumisano City, Osaka, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Shimotsuma
authors.affiliation: Laboratory of Veterinary Pathology, Osaka Prefecture University, Izumisano City, Osaka, Japan.
authors.forename: Miyuu
authors.initials: M
authors.lastname: Tanaka
authors.affiliation: Laboratory of Veterinary Pathology, Osaka Prefecture University, Izumisano City, Osaka, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Izawa
authors.affiliation: Laboratory of Veterinary Pathology, Osaka Prefecture University, Izumisano City, Osaka, Japan.
authors.forename: Jyoji
authors.initials: J
authors.lastname: Yamate
authors.affiliation: Laboratory of Veterinary Pathology, Osaka Prefecture University, Izumisano City, Osaka, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Kuwamura
mesh_terms: D000818:Animals; D003711:Demyelinating Diseases / Q000235:genetics* / Q000473:pathology; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008297:Male; D046228:Microarray Analysis; D009186:Myelin Sheath / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D017346:Protein Serine-Threonine Kinases / Q000037:antagonists & inhibitors* / Q000235:genetics; D051381:Rats; D011922:Rats, Mutant Strains; D055647:Rats, Transgenic; D015854:Up-Regulation / Q000235:genetics
chemical_list: C502443:Trib3 protein, rat; D017346:Protein Serine-Threonine Kinases