title: Pin1 Knockout Mice: A Model for the Study of Tau Pathology in Alzheimer's Disease.
abstract: Pin1 knockout in mice causes age-dependent neuropathy characterized by motor and behavioral deficits, tau hyper phosphorylation, tau filament formation, and neuronal degradation. Here, we describe the methods with mouse behavior test, immunostaining, and immunoblotting to detect many aspects of neurodegeneration in Pin1 knockout mice.
authors.affiliation: Division of Translational Therapeutics, Department of Medicine and the Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, 3 Blackfan Circle, Boston, MA, 02115, USA.
authors.forename: Asami
authors.initials: A
authors.lastname: Kondo
authors.affiliation: Division of Translational Therapeutics, Department of Medicine and the Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, 3 Blackfan Circle, Boston, MA, 02115, USA.
authors.forename: Onder
authors.initials: O
authors.lastname: Albayram
authors.affiliation: Division of Translational Therapeutics, Department of Medicine and the Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, 3 Blackfan Circle, Boston, MA, 02115, USA. xzhou@bidmc.harvard.edu.
authors.forename: Xiao Zhen
authors.initials: XZ
authors.lastname: Zhou
authors.affiliation: Division of Translational Therapeutics, Department of Medicine and the Cancer Center, Beth Israel Deaconess Medical Center, Harvard Medical School, 3 Blackfan Circle, Boston, MA, 02115, USA. klu@bidmc.harvard.edu.
authors.forename: Kun Ping
authors.initials: KP
authors.lastname: Lu
mesh_terms: D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D000067290:Behavior Rating Scale; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D051379:Mice; D018345:Mice, Knockout; D000072340:NIMA-Interacting Peptidylprolyl Isomerase / Q000172:deficiency / Q000235:genetics*; D024801:Tauopathies / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D016875:tau Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D000072340:NIMA-Interacting Peptidylprolyl Isomerase; D016875:tau Proteins
keywords: Alzheimer’s disease; Behavior test; Cis P-tau; Immunoblotting; Immunohistochemistry; Pin1