title: Prediction of atorvastatin plasmatic concentrations in healthy volunteers using integrated pharmacogenetics sequencing.
abstract: OBJECTIVE
  To use variants found by next-generation sequencing to predict atorvastatin plasmatic concentration profiles (AUC) in healthy volunteers.
  
  
  METHODS
  A total of 60 healthy Mexican volunteers were enrolled in this study. We used variants with a predicted functional effect across 20 genes involved in atorvastatin metabolism to construct a regression model using a support vector approach with a radial basis function kernel to predict AUC refining it afterwards in order to explain a greater extent of the variance.
  
  
  RESULTS
  The final support vector regression model using 60 variants (including six novel variants) explained 94.52% of the variance in atorvastatin AUC.
  
  
  CONCLUSIONS
  An integrated analysis of several genes known to intervene in the different steps of metabolism is required to predict atorvastatin's AUC.
authors.affiliation: Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F. C.P. 14610, Mexico.
authors.forename: Omar Fernando
authors.initials: OF
authors.lastname: Cruz-Correa
authors.affiliation: Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Madero, Col. Mitras Centro, Monterrey, Nuevo León, C.P. 64640, Mexico.|División Ciencias de la Salud, Departamento de Ciencias Básicas, Centro de Diagnóstico Molecular y Medicina Personalizada, Universidad de Monterrey, Ave. Ignacio Morones Prieto Pte. 4500, Col. Jesús M. Garza, San Pedro Garza García, Nuevo León, C.P. 66238, Mexico.
authors.forename: Rafael Baltazar Reyes
authors.initials: RB
authors.lastname: León-Cachón
authors.affiliation: Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Ave. Madero, Col. Mitras Centro, Monterrey, Nuevo León, C.P. 64640, Mexico.|Vitagénesis, SA de CV., Col. Colinas de San Jerónimo. Monterrey, Nuevo León, C.P. 64630, Mexico.
authors.forename: Hugo Alberto
authors.initials: HA
authors.lastname: Barrera-Saldaña
authors.affiliation: Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Delegación Tlalpan, México, D.F. C.P. 14610, Mexico.|Instituto de Biotecnología, Universidad Nacional Autónoma de México, Avenida Universidad 2001, Cuernavaca, Morelos, C.P. 62210, Mexico.
authors.forename: Xavier
authors.initials: X
authors.lastname: Soberón
mesh_terms: D000293:Adolescent; D000328:Adult; D000069059:Atorvastatin / Q000097:blood* / Q000493:pharmacokinetics; D005544:Forecasting; D064368:Healthy Volunteers; D006801:Humans; D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors / Q000097:blood* / Q000493:pharmacokinetics; D008297:Male; D008800:Mexico / Q000453:epidemiology; D008875:Middle Aged; D010597:Pharmacogenetics / Q000379:methods*; D000071184:Pharmacogenomic Variants / Q000235:genetics*; D055815:Young Adult
chemical_list: D019161:Hydroxymethylglutaryl-CoA Reductase Inhibitors; D000069059:Atorvastatin
keywords: Next-Gen sequencing; atorvastatin; pharmacokinetics