title: Bisphenol A influences oestrogen- and thyroid hormone-regulated thyroid hormone receptor expression in rat cerebellar cell culture.
abstract: Thyroid hormones (THs) and oestrogens are crucial in the regulation of cerebellar development. TH receptors (TRs) mediate these hormone effects and are regulated by both hormone families. We reported earlier that THs and oestradiol (E2) determine TR levels in cerebellar cell culture. Here we demonstrate the effects of low concentrations (10-10M) of the endocrine disruptor (ED) bisphenol A (BPA) on the hormonal (THs, E2) regulation of TRα,β in rat cerebellar cell culture. Primary cerebellar cell cultures, glia-containing and glia-destroyed, were treated with BPA or a combination of BPA and E2and/or THs. Oestrogen receptor and TH receptor mRNA and protein levels were determined by real-time qPCR and Western blot techniques. The results show that BPA alone decreases, while BPA in combination with THs and/or E2increases TR mRNA expression. In contrast, BPA alone increased receptor protein expressions, but did not further increase them in combination with THs and/or E2. The modulatory effects of BPA were mediated by the glia; however, the degree of changes also depended on the specific hormone ligand used. The results signify the importance of the regulatory mechanisms interposed between transcription and translation and raise the possibility that BPA could act to influence nuclear hormone receptor levels independently of ligand-receptor interaction.
authors.affiliation: Department of Physiology and Biochemistry, University of Veterinary Medicine , Budapest , Hungary.
authors.forename: Virág
authors.initials: V
authors.lastname: Somogyi
authors.affiliation: Department of Animal Physiology and Animal Health, Faculty of Agricultural and Environmental Sciences, Szent István University , Páter Károly u. 1, H-2100 Gödöllő , Hungary.|Section of Comparative Medicine, Yale University School of Medicine , New Haven, CT , USA.
authors.forename: Tamás L
authors.initials: TL
authors.lastname: Horváth
authors.affiliation: Department of Physiology and Biochemistry, University of Veterinary Medicine , Budapest , Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Tóth
authors.affiliation: Department of Physiology and Biochemistry, University of Veterinary Medicine , Budapest , Hungary.
authors.forename: Tibor
authors.initials: T
authors.lastname: Bartha
authors.affiliation: Department of Physiology and Biochemistry, University of Veterinary Medicine , Budapest , Hungary.
authors.forename: László Vilmos
authors.initials: LV
authors.lastname: Frenyó
authors.affiliation: Department of Physiology and Biochemistry, University of Veterinary Medicine , Budapest , Hungary.
authors.forename: Dávid Sándor
authors.initials: DS
authors.lastname: Kiss
authors.affiliation: Department of Physiology and Biochemistry, University of Veterinary Medicine , Budapest , Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Jócsák
authors.affiliation: Department of Animal Physiology and Animal Health, Faculty of Agricultural and Environmental Sciences, Szent István University , Páter Károly u. 1, H-2100 Gödöllő , Hungary.
authors.forename: Annamária
authors.initials: A
authors.lastname: Kerti
authors.affiliation: Reproductive Biology Research, New York University , New York, NY , USA.
authors.forename: Frederick
authors.initials: F
authors.lastname: Naftolin
authors.affiliation: Department of Animal Physiology and Animal Health, Faculty of Agricultural and Environmental Sciences, Szent István University , Páter Károly u. 1, H-2100 Gödöllő , Hungary.|Section of Comparative Medicine, Yale University School of Medicine , New Haven, CT , USA.
authors.forename: Attila
authors.initials: A
authors.lastname: Zsarnovszky
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000494:pharmacology*; D002478:Cells, Cultured; D002531:Cerebellum / Q000166:cytology*; D052244:Endocrine Disruptors / Q000494:pharmacology; D004967:Estrogens / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism; D009474:Neurons / Q000187:drug effects* / Q000378:metabolism; D010636:Phenols / Q000494:pharmacology*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D011988:Receptors, Thyroid Hormone / Q000235:genetics / Q000378:metabolism*; D013963:Thyroid Hormones / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D004967:Estrogens; D010636:Phenols; D012333:RNA, Messenger; D011988:Receptors, Thyroid Hormone; D013963:Thyroid Hormones; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Endocrine disruptors; bisphenol A; rat cerebellum; thyroid receptor; transcription; translation