title: Formation of wood secondary cell wall may involve two type cellulose synthase complexes in Populus.
abstract: Cellulose biosynthesis is mediated by cellulose synthases (CesAs), which constitute into rosette-like cellulose synthase complexe (CSC) on the plasma membrane. Two types of CSCs in Arabidopsis are believed to be involved in cellulose synthesis in the primary cell wall and secondary cell walls, respectively. In this work, we found that the two type CSCs participated cellulose biosynthesis in differentiating xylem cells undergoing secondary cell wall thickening in Populus. During the cell wall thickening process, expression of one type CSC genes increased while expression of the other type CSC genes decreased. Suppression of different type CSC genes both affected the wall-thickening and disrupted the multilaminar structure of the secondary cell walls. When CesA7A was suppressed, crystalline cellulose content was reduced, which, however, showed an increase when CesA3D was suppressed. The CesA suppression also affected cellulose digestibility of the wood cell walls. The results suggest that two type CSCs are involved in coordinating the cellulose biosynthesis in formation of the multilaminar structure in Populus wood secondary cell walls.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xi
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: Song
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
authors.forename: Jiayan
authors.initials: J
authors.lastname: Sun
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Shen
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, 200032, China. lgli@sibs.ac.cn.
authors.forename: Laigeng
authors.identifier: http://orcid.org/0000-0001-6924-4431
authors.initials: L
authors.lastname: Li
mesh_terms: D015153:Blotting, Western; D002473:Cell Wall / Q000235:genetics* / Q000378:metabolism / Q000648:ultrastructure; D002482:Cellulose / Q000096:biosynthesis; D015971:Gene Expression Regulation, Enzymologic; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D007527:Isoenzymes / Q000235:genetics / Q000378:metabolism; D046529:Microscopy, Electron, Transmission; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D032107:Populus / Q000201:enzymology / Q000235:genetics* / Q000378:metabolism; D034622:RNA Interference; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014934:Wood / Q000235:genetics* / Q000378:metabolism; D052584:Xylem / Q000201:enzymology / Q000235:genetics / Q000378:metabolism
chemical_list: D007527:Isoenzymes; D010940:Plant Proteins; D002482:Cellulose; D005964:Glucosyltransferases; C478648:cellulose synthase
keywords: Cellulose synthase; Populus; Secondary cell wall; Wood formation