title: Surgical repair of annulus defect with biomimetic multilamellar nano/microfibrous scaffold in a porcine model.
abstract: Annulus defect is associated with reherniation and disc degeneration after discectomy; currently there is no effective treatment that addresses this problem. The annulus is a hierarchical lamellar structure, where each lamella consists of aligned collagen fibres, which are parallel and tilted at 30° to the spinal axis. In this study, a biomimetic biodegradable scaffold consisting of multilamellar nano/microfibres, sharing nanotopography and microporosity similar to the native lamellar structure, was assessed in a porcine model, aided by sealing with fascia and medical glue and subsequent suture fixation. After 6- and 12-week observation, we found that this treatment restored nucleus volume and slowed down disc degeneration, as indicated by magnetic resonance imaging of T1/T2-weighted, T2-mapping, T1-ρ imaging. Histological analysis showed aligned collagen fibres organized in the scaffold and integrated with surrounding native annulus tissue. The autologous bone marrow concentrate-seeded scaffolds showed slightly earlier collagen fibre formation at 6 weeks. This novel treatment could efficiently close the annulus defect with newly formed, organized and integrated collagen fibres in a porcine model. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.|Department of Orthopedic Surgery, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, China.
authors.forename: R
authors.initials: R
authors.lastname: Kang
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.
authors.forename: H
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Orthopedic Surgery, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Xi
authors.affiliation: The MR Research Centre, Aarhus University Hospital, Skejby, Aarhus, Denmark.
authors.forename: S
authors.initials: S
authors.lastname: Ringgard
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.
authors.forename: A
authors.initials: A
authors.lastname: Baatrup
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.
authors.forename: K
authors.initials: K
authors.lastname: Rickers
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Sun
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.
authors.forename: D Q S
authors.initials: DQS
authors.lastname: Le
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Orthopedic Surgery, Jiangsu Province Hospital on Integration of Chinese and Western Medicine, Nanjing, China.
authors.forename: L
authors.initials: L
authors.lastname: Xie
authors.affiliation: Nanjing University of Chinese Medicine Hanlin College, Taizhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Engineering, Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.
authors.forename: M
authors.initials: M
authors.lastname: Chen
authors.affiliation: Orthopaedic Research Lab, Aarhus University, Aarhus, Denmark.
authors.forename: C
authors.initials: C
authors.lastname: Bünger
mesh_terms: D000818:Animals; D000070616:Annulus Fibrosus / Q000473:pathology / Q000601:surgery*; D001672:Biocompatible Materials / Q000494:pharmacology; D032701:Biomimetics / Q000379:methods*; D024043:Collagen Type II / Q000378:metabolism; D005260:Female; D008279:Magnetic Resonance Imaging; D023421:Models, Animal; D057139:Nanofibers / Q000737:chemistry* / Q000648:ultrastructure; D013552:Swine; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D024043:Collagen Type II