title: New Sweet-Tasting C21-Pregnane Glycosides from Pericarps of Myriopteron extensum.
abstract: Ten novel C21 pregnane glycosides, extensumside C-L (1-10), were isolated as highly sweet-tasting substances from the edible pericarps of Myriopteron extensum (Wight) K. Schum by sensory-guided fractionation and purification. Their structures were determined through 1D and 2D NMR, such as HSQC, HMBC,1H-1H COSY, HSQC-TOCSY, and ROESY, as well as other spectroscopic analysis combined with chemical evidence. These compounds shared the same aglycone, 3β,16α-dihydroxy-pregn-5-en-20-one, and contained the deoxysugar chain and the glucose chain which were linked to C-3 and C-16 of the aglycone, respectively. The sweetness potency was evaluated by a human sensory panel test and preliminary structure-taste relationship was discussed. The sweetness intensities of these compounds are between 50 and 400 times greater than that of sucrose. Furthermore, quantitation analyses of compounds 1, 3, 4, and 6 in different parts of M. extensum indicated that the concentrations of these sweet components in the pericarps are obviously higher than those in stems and roots.
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences , Lanhei Road 132, Kunming 650201, Yunnan, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Guo
authors.initials: G
authors.lastname: Sun
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences , Lanhei Road 132, Kunming 650201, Yunnan, China.|University of Chinese Academy of Sciences , Beijing 100049, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Dai
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences , Lanhei Road 132, Kunming 650201, Yunnan, China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences , Lanhei Road 132, Kunming 650201, Yunnan, China.
authors.forename: Zhi-Jian
authors.initials: ZJ
authors.lastname: Li
authors.affiliation: Key Laboratory of Economic Plants and Biotechnology, Yunnan Key Laboratory for Wild Plant Resources, Kunming Institute of Botany, Chinese Academy of Sciences , Lanhei Road 132, Kunming 650201, Yunnan, China.
authors.forename: Zhi-Zhi
authors.initials: ZZ
authors.lastname: Du
mesh_terms: D000328:Adult; D029062:Apocynaceae / Q000737:chemistry*; D005260:Female; D005638:Fruit / Q000737:chemistry; D006027:Glycosides / Q000737:chemistry* / Q000302:isolation & purification; D006801:Humans; D009682:Magnetic Resonance Spectroscopy; D008297:Male; D008875:Middle Aged; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D011278:Pregnanes / Q000737:chemistry* / Q000302:isolation & purification; D013549:Sweetening Agents / Q000737:chemistry* / Q000302:isolation & purification; D013649:Taste
chemical_list: D006027:Glycosides; D010936:Plant Extracts; D011278:Pregnanes; D013549:Sweetening Agents; C574437:pregnane glycoside
keywords: C21-pregnane glycosides; highly sweet natural products; sensory evaluation; sweet intensity