title: Hypoxia facilitates epithelial-mesenchymal transition-mediated rectal cancer progress.
abstract: Rectal cancer is a commonly observed tumor in clinics, and epithelial-mesenchymal transition (EMT) is very important for tumor invasion and metastasis. We established a rectal cancer HCT-116 cell hypoxia model and detected cell proliferation, invasion, and EMT-related protein expression in this model, aiming to analyze the effect of hypoxia on rectal cancer cell EMT. Rectal cancer cell line HCT-116 was cultured in normoxic, hypoxic, or anaerobic environment, and hypoxia-inducible factor-1α (HIF-1α) mRNA expression was detected in the cells by real-time PCR. Cell proliferation was tested by MTT assay; cell invasion was determined by transwell assay, and HIF-1α, epithelial-cadherin, and Snail protein levels were evaluated by western blot analysis. HIF-1α mRNA level significantly increased in the anaerobic group compared to that in the normoxic and hypoxic groups (P < 0.05). HCT-116 cell proliferation in the anaerobic group was obviously higher than that in the other two groups, with the hypoxic group showing stronger proliferative ability than the normoxic group (P < 0.05). Compared to the normoxic group, the HCT-116 cells demonstrated enhanced cell invasion and migration in hypoxic and anaerobic groups. HIF-1α and Snail expressions were upregulated, whereas epithelial-cadherin expression had declined in the hypoxic and anaerobic groups, compared to those in the normal control (P < 0.05). Therefore, hypoxia promoted rectal cancer cell progress by increasing HIF-1α to induce EMT.
authors.affiliation: School of Nursing, The Third Xiangya Hospital of Central South University, China.
authors.forename: L L
authors.initials: LL
authors.lastname: Sun
authors.affiliation: School of Nursing, The Third Xiangya Hospital of Central South University, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Song
authors.affiliation: School of Nursing, The Third Xiangya Hospital of Central South University, China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Li
authors.affiliation: School of Nursing, The Third Xiangya Hospital of Central South University, China siyuantangqwe@sina.com.
authors.forename: S Y
authors.initials: SY
authors.lastname: Tang
mesh_terms: D015687:Cell Hypoxia; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000235:genetics* / Q000378:metabolism*; D012004:Rectal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D000071250:Snail Family Transcription Factors / Q000378:metabolism*
chemical_list: C497442:HIF1A protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D000071250:Snail Family Transcription Factors