title: Self-Replicating RNA.
abstract: Self-replicating RNA derived from the genomes of positive strand RNA viruses represents a powerful tool for both molecular studies on virus biology and approaches to novel safe and effective vaccines. The following chapter summarizes the principles how such RNAs can be established and used for design of vaccines. Due to the large variety of strategies needed to circumvent specific pitfalls in the design of such constructs the technical details of the experiments are not described here but can be found in the cited literature.
authors.affiliation: Institut für molekulare Virologie und Zellbiologie, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493, Greifswald-Insel Riems, Germany. Birke.Tews@fli.de.
authors.forename: Birke Andrea
authors.initials: BA
authors.lastname: Tews
authors.affiliation: Institut für Immunologie, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493, Greifswald-Insel Riems, Germany. gregor.meyers@fli.de.
authors.forename: Gregor
authors.initials: G
authors.lastname: Meyers
mesh_terms: D000818:Animals; D006801:Humans; D012328:RNA Viruses / Q000235:genetics* / Q000276:immunology*; D012367:RNA, Viral / Q000235:genetics* / Q000276:immunology*; D012093:Replicon / Q000235:genetics / Q000276:immunology; D014612:Vaccines / Q000235:genetics / Q000276:immunology*
chemical_list: D012367:RNA, Viral; D014612:Vaccines
keywords: Alphavirus; Classical swine fever virus; Flavivirus; Pestivirus; Positive strand RNA virus; Self-replicating RNA