title: Vinpocetine Inhibited the CpG Oligodeoxynucleotide-induced Immune Response in Plasmacytoid Dendritic Cells.
abstract: Plasmacytoid dendritic cells (pDCs) exert dual roles in immune responses through inducing inflammation and maintaining immune tolerance. A switch of pDC phenotype from pro-inflammation to tolerance has therapeutic promise in the treatment of autoimmune diseases. Vinpocetine, a vasoactive vinca alkaloid extracted from the periwinkle plant, has recently emerged as an immunomodulatory agent. In this study, we evaluated the effect of vinpocetine on phenotype of pDCs isolated from C57BL/6 mice and explored its possible mechanism. Our data showed that vinpocetine significantly downregulated the expression of CD40, CD80, and CD86 on pDCs and increased the expression of translocator protein (TSPO), the specific receptor of vinpocetine, in pDCs. Vinpocetine significantly inhibited the Toll-like receptor 9 signaling pathway and reduced the secretion of related cytokines in pDCs through TSPO. Furthermore, viability of pDCs was significantly promoted by vinpocetine. These findings imply that vinpocetine serves as an immunomodulatory agent for pDCs and may be applied for the treatment of pDCs-related autoimmune diseases.
authors.affiliation: a Department of Neurology , Affiliated Hospital of Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Xungang
authors.initials: X
authors.lastname: Feng
authors.affiliation: a Department of Neurology , Affiliated Hospital of Jining Medical University , Jining , Shandong Province , People's Republic of China.|b Department of Central Laboratory , Affiliated Hospital of Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: a Department of Neurology , Affiliated Hospital of Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Yanlei
authors.initials: Y
authors.lastname: Hao
authors.affiliation: c Department of Immunology , Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Ma
authors.affiliation: c Department of Immunology , Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Dai
authors.affiliation: d Department of Anus & Intestine Surgery and Pain Medicine , Jinxiang Hongda Hospital , Jining , Shandong Province , People's Republic of China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Liang
authors.affiliation: a Department of Neurology , Affiliated Hospital of Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Yantao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: a Department of Neurology , Affiliated Hospital of Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Xiangyuan
authors.initials: X
authors.lastname: Li
authors.affiliation: a Department of Neurology , Affiliated Hospital of Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Song
authors.affiliation: c Department of Immunology , Jining Medical University , Jining , Shandong Province , People's Republic of China.
authors.forename: Chuanping
authors.initials: C
authors.lastname: Si
mesh_terms: D000818:Animals; D001327:Autoimmune Diseases / Q000188:drug therapy*; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D018899:CpG Islands / Q000235:genetics; D016207:Cytokines / Q000378:metabolism; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology; D006801:Humans; D007155:Immunologic Factors / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D009838:Oligodeoxyribonucleotides / Q000276:immunology; D018079:Receptors, GABA / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D051217:Toll-Like Receptor 9 / Q000378:metabolism; D031053:Vinca / Q000276:immunology*; D014748:Vinca Alkaloids / Q000494:pharmacology*
chemical_list: C463819:Bzrp protein, mouse; D016207:Cytokines; D007155:Immunologic Factors; D009838:Oligodeoxyribonucleotides; D018079:Receptors, GABA; C495370:Tlr9 protein, mouse; D051217:Toll-Like Receptor 9; D014748:Vinca Alkaloids; C013983:vinpocetine
keywords: Immunomodulation; plasmacytoid dendritic cells; vinpocetine