title: Gallium containing composites as a tunable material to understand neuronal behavior under variable stiffness and radiation conditions.
abstract: We report a composite biomaterial containing nanostructured GaOOH and Matrigel™ that can be modulated with respect to its stiffness and radiosensitization properties. A variety of concentrations of GaOOH were added to the composite to alter the mechanical properties of the material as well as to tune the radiosensitizing properties to the composite. PC-12 cells were used to study the combined effects of different stimuli on cell behavior. NGF was given to the cells to record their morphology as well as viability. An increase in the substrate stiffness caused an increase in neurite outgrowth but a decrease in cell viability. In addition, increasing the radiation dose decreased neurite outgrowth but increased cell viability when radiosensitizing particles were present. A subtractive effect between radiosensitizing and mechanical stimuli was observed when PC-12 cells were grown on the GaOOH containing composite.
authors.affiliation: Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Nora G
authors.initials: NG
authors.lastname: Berg
authors.affiliation: Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Brady L
authors.initials: BL
authors.lastname: Pearce
authors.affiliation: Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27695, USA.
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Rohrbaugh
authors.affiliation: Materials Science and Engineering, University of New South Wales, Sydney, Australia.
authors.forename: Lin
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Clinical Sciences (College of Veterinary Medicine), Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27606, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Nolan
authors.affiliation: Department of Materials Science and Engineering, North Carolina State University, Raleigh, NC 27695, USA. Electronic address: ivanisevic@ncsu.edu.
authors.forename: Albena
authors.initials: A
authors.lastname: Ivanisevic
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D005708:Gallium / Q000737:chemistry / Q000494:pharmacology; D053761:Nanocomposites / Q000737:chemistry*; D020932:Nerve Growth Factor / Q000737:chemistry / Q000494:pharmacology; D016501:Neurites / Q000378:metabolism*; D016716:PC12 Cells; D011838:Radiation-Sensitizing Agents / Q000737:chemistry / Q000494:pharmacology; D051381:Rats
chemical_list: D011838:Radiation-Sensitizing Agents; D020932:Nerve Growth Factor; D005708:Gallium
keywords: Gallium; Multifunctional; Radiation; Radiosensitizing; Stiffness