title: Transcript and hormone analyses reveal the involvement of ABA-signalling, hormone crosstalk and genotype-specific biological processes in cold-shock response in wheat.
abstract: The effect of one-day cold-shock on the transcriptome and phytohormones (auxin, cytokinins, abscisic, jasmonic and salicylic acids) was characterised in freezing-sensitive (Chinese Spring), highly freezing-tolerant (Cheyenne) and moderately freezing-tolerant (Chinese Spring substituted with Cheyenne's 5A chromosome) wheat genotypes. Altogether, 636 differentially expressed genes responding to cold-shock were identified. Defence genes encoding LEA proteins, dehydrins, chaperons and other temperature-stress responsive proteins were up-regulated in a genotype-independent manner. Abscisic acid was up-regulated by cold accompanied by adherent expression of its metabolic genes. Data revealed the involvement of particular routes within ABA-dependent signalling in response to cold-shock in the examined genotypes. Cold-shock affected gene expression along carbohydrate metabolic pathways. In photosynthesis, cold-shock changed the expression of a number of genes in the same way as it was previously reported for ABA. Overrepresentation analysis of the differentially expressed genes supported the ABA-signalling and carbohydrate metabolism results, and revealed some pronounced biological process GO categories associated with the cold-shock response of the genotypes. Protein network analysis indicated differences between the genotypes in the information flow along their signal perception and transduction, suggesting different biochemical and cellular strategies in their reaction to cold-shock.
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462 Martonvásár, Brunszvik u. 2, Hungary; Festetics Doctoral School, Georgikon Faculty, University of Pannonia, 8360, Keszthely, Festetics u. 7, Hungary. Electronic address: kalapos.balazs@agrar.mta.hu.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kalapos
authors.affiliation: Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Rozvojová 263, 165 02 Praha 6, Czech Republic. Electronic address: dobrev@ueb.cas.cz.
authors.forename: Petre
authors.initials: P
authors.lastname: Dobrev
authors.affiliation: Agricultural Biotechnology Institute, NAIK, 2100 Gödöllő, Szent-Györgyi Albert u. 4, Hungary. Electronic address: tn5@sanger.ac.uk.
authors.forename: Tibor
authors.initials: T
authors.lastname: Nagy
authors.affiliation: Department of Genetics and Plant Breeding, Crop Research Institute, Drnovska 507/73 16106 Prague 6, Czech Republic. Electronic address: vitamvas@vurv.cz.
authors.forename: Pavel
authors.initials: P
authors.lastname: Vítámvás
authors.affiliation: Institute of Plant Biology, Biological Research Centre, Hungarian Academy of Sciences, 6726, Szeged, Temesvári krt. 62, Hungary. Electronic address: arthur@brc.hu.
authors.forename: János
authors.initials: J
authors.lastname: Györgyey
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462 Martonvásár, Brunszvik u. 2, Hungary. Electronic address: kocsy.gabor@agrar.mta.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Kocsy
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462 Martonvásár, Brunszvik u. 2, Hungary; Agricultural Biotechnology Institute, NAIK, 2100 Gödöllő, Szent-Györgyi Albert u. 4, Hungary. Electronic address: marincs.ferenc@abc.naik.hu.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Marincs
authors.affiliation: Agricultural Institute, Centre for Agricultural Research, Hungarian Academy of Sciences, 2462 Martonvásár, Brunszvik u. 2, Hungary; Festetics Doctoral School, Georgikon Faculty, University of Pannonia, 8360, Keszthely, Festetics u. 7, Hungary. Electronic address: galibag@agrar.mta.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Galiba
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism*; D050260:Carbohydrate Metabolism / Q000235:genetics; D003080:Cold Temperature; D005838:Genotype; D010937:Plant Growth Regulators / Q000378:metabolism; D020239:Receptor Cross-Talk; D015398:Signal Transduction; D059467:Transcriptome; D014908:Triticum / Q000235:genetics / Q000378:metabolism*
chemical_list: D010937:Plant Growth Regulators; D000040:Abscisic Acid
keywords: ABA-Signalling; Carbon metabolism; Freezing-tolerance; Gene ontology; Plant hormones; Short-term cold-shock; Triticum aestivum