title: Virgibacillus ainsalahensis sp. nov., a Moderately Halophilic Bacterium Isolated from Sediment of a Saline Lake in South of Algeria.
abstract: A Gram-positive, moderately halophilic, endospore-forming bacterium, designated MerVT, was isolated from a sediment sample of a saline lake located in Ain Salah, south of Algeria. The cells were rod shaped and motile. Isolate MerVTgrew at salinity interval of 0.5-25% NaCl (optimum, 5-10%), pH 6.0-12.0 (optimum, 8.0), and temperature between 10 and 40 °C (optimum, 30 °C).The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, a glycolipid, a phospholipid, and two lipids, and MK-7 is the predominant menaquinone. The predominant cellular fatty acids were anteiso C15:0and anteiso C17:0. The DNA G+C content was 45.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain MerVTwas most closely related to Virgibacillus halodenitrificans (gene sequence similarity of 97.0%). On the basis of phenotypic, chemotaxonomic properties, and phylogenetic analyses, strain MerVT(=DSM = 28944T) should be placed in the genus Virgibacillus as a novel species, for which the name Virgibacillus ainsalahensis is proposed.
authors.affiliation: Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria.
authors.forename: Meriam
authors.initials: M
authors.lastname: Amziane
authors.affiliation: Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria.
authors.forename: Amel
authors.initials: A
authors.lastname: Darenfed-Bouanane
authors.affiliation: Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Abderrahmani
authors.affiliation: Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria.
authors.forename: Okba
authors.initials: O
authors.lastname: Selama
authors.affiliation: Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria.
authors.forename: Lydia
authors.initials: L
authors.lastname: Jouadi
authors.affiliation: Aix Marseille Université, IRD, Université de Toulon, CNRS, Mediterranean Institute of Oceanography (MIO), UM 110, Case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Cayol
authors.affiliation: Microbiology Group, Laboratory of Cellular and Molecular Biology, Faculty of Biology, USTHB, BP 32, El Alia, Bab Ezzouar, Algiers, Algeria.
authors.forename: Farida
authors.initials: F
authors.lastname: Nateche
authors.affiliation: Aix Marseille Université, IRD, Université de Toulon, CNRS, Mediterranean Institute of Oceanography (MIO), UM 110, Case 925, 163 Avenue de Luminy, 13288, Marseille Cedex 9, France. marie-laure.fardeau@univ-amu.fr.
authors.forename: Marie-Laure
authors.initials: ML
authors.lastname: Fardeau
mesh_terms: D000462:Algeria; D001482:Base Composition; D016000:Cluster Analysis; D003600:Cytosol / Q000737:chemistry; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005227:Fatty Acids / Q000032:analysis; D019015:Geologic Sediments / Q000382:microbiology*; D006017:Glycolipids / Q000032:analysis; D060106:Lakes; D008124:Locomotion; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012965:Sodium Chloride / Q000378:metabolism; D013696:Temperature; D058679:Virgibacillus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000502:physiology; D024482:Vitamin K 2 / Q000032:analysis
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D006017:Glycolipids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D012965:Sodium Chloride