title: Antibacterial Combination of Oleoresin from Copaifera multijuga Hayne and Biogenic Silver Nanoparticles Towards Streptococcus agalactiae.
abstract: BACKGROUND
  Streptococcus agalactiae (group B Streptococcus - GBS) remains a leading cause of neonatal infections and an important cause of invasive infections in adults with underlying conditions.
  
  
  METHODS
  This study evaluated for the first time the effect of an oleoresin collected from Copaifera multijuga Hayne (copaiba oil) alone or in combination with silver nanoparticles produced by green synthesis using Fusarium oxysporum (AgNPbio) against planktonic and sessile cells of GBS isolated from colonized women.
  
  
  RESULTS
  Copaiba oil showed a dose-dependent bactericidal activity against planktonic GBS strains, including those resistant to erythromycin and/or clindamycin. Scanning and transmission electron microscopy of GBS treated with copaiba oil revealed morphological and ultrastructural alterations, displaying disruption of the cell wall and decreased electron density due to leakage of cytoplasmic content. Copaiba oil also exhibited antibacterial activity against biofilms of GBS strains, inhibiting their formation as well as the viability of mature biofilms. In addition, the combination of copaiba oil with AgNPbio resulted in a synergistic effect against planktonic cells and biofilm formation, reducing the minimal inhibitory concentration values of both compounds. No hemolytic activity was detected for both compounds.
  
  
  CONCLUSIONS
  These results indicate the potential of copaiba oil, alone or in combination with AgNPbio, for the development of new alternative strategies for controlling GBS infections.
authors.forename: Eliane S
authors.initials: ES
authors.lastname: Otaguiri
authors.forename: Ana E B
authors.initials: AEB
authors.lastname: Morguette
authors.forename: Renata P
authors.initials: RP
authors.lastname: Biasi-Garbin
authors.forename: Alexandre T
authors.initials: AT
authors.lastname: Morey
authors.forename: Cesar A C
authors.initials: CAC
authors.lastname: Lancheros
authors.forename: Danielle
authors.initials: D
authors.lastname: Kian
authors.forename: Admilton G
authors.initials: AG
authors.lastname: de Oliveira
authors.forename: Gilselena
authors.initials: G
authors.lastname: Kerbauy
authors.forename: Marcia R E
authors.initials: MRE
authors.lastname: Perugini
authors.forename: Nelson
authors.initials: N
authors.lastname: Duran
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.forename: Valdir F
authors.initials: VF
authors.lastname: da Veiga
authors.forename: Gerson
authors.initials: G
authors.lastname: Nakazato
authors.forename: Phileno
authors.initials: P
authors.lastname: Pinge-Filho
authors.forename: Lucy M
authors.initials: LM
authors.lastname: Yamauchi
authors.affiliation: Departamento de Microbiologia, Centro de Ciencias Biologicas, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, PR 445, km 380, CEP: 86057970, Londrina, Parana, Brazil.
authors.forename: Sueli F
authors.initials: SF
authors.lastname: Yamada-Ogatta
mesh_terms: D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018441:Biofilms / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D004357:Drug Synergism; D007887:Fabaceae / Q000737:chemistry*; D005260:Female; D006801:Humans; D020100:Hydrogels / Q000302:isolation & purification / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D012007:Rectum / Q000382:microbiology; D012834:Silver / Q000302:isolation & purification / Q000494:pharmacology* / Q000633:toxicity; D018030:Silver Compounds / Q000302:isolation & purification / Q000494:pharmacology; D013292:Streptococcus agalactiae / Q000187:drug effects* / Q000302:isolation & purification; D014621:Vagina / Q000382:microbiology
chemical_list: D000900:Anti-Bacterial Agents; D020100:Hydrogels; D010936:Plant Extracts; C500575:SilvaSorb; D018030:Silver Compounds; C018620:oleoresins; D012834:Silver
keywords: Fusarium oxysporum; Group B Streptococcus; antimicrobial synergism; biofilm; biological silver nanoparticles; clindamycin and erythromycin resistance; copaiba oil