title: Preparation of dual-responsive hybrid fluorescent nano probe based on graphene oxide and boronic acid/BODIPY-conjugated polymer for cell imaging.
abstract: Here, we report a pH- and thermo-responsive fluorescent nanomaterial of functionalized reduced graphene oxide (rGO) with cross-linked polymer produced via catechol-boronate diol binding mechanism. When conjugated with the hydrophobic dye boron dipyrromethane (BODIPY), this material can act as a dual-responsive nanoplatform for cells imaging. 2-Chloro-3',4'-dihydroxyacetophenone (CCDP)-quaternized-poly(dimethylaminoethyl methacrylate-co-N-isopropylacrylamide) [C-PDN] was cross-linked with BODIPY and 4-chlorophenyl boronic acid (BA)-quaternized-poly(ethylene glycol)-g-poly(dimethylaminoethyl methacrylate-co-N-isopropylacrylamide) [BB-PPDN]. The GO was then reduced by the catechol group in the cross-linked polymer to synthesize rGO nanoparticles, which able to stabilize the quenching mechanism. This nanoplatform exhibits intense fluorescence at acidic pH and low fluorescence at physiological pH. Confocal laser scanning microscopy (CLSM) images shows bright fluorescence at lysosomal pH and total quench at physiological pH. Therefore, we have successfully developed a promising sensitive bio-imaging probe for identifying cancer cells.
authors.affiliation: Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.lastname: Khoerunnisa
authors.affiliation: Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Eun Bi
authors.initials: EB
authors.lastname: Kang
authors.affiliation: Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Zihnil Adha Islamy
authors.initials: ZAI
authors.lastname: Mazrad
authors.affiliation: Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Gibaek
authors.initials: G
authors.lastname: Lee
authors.affiliation: Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea; Department of Polymer Science and Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea.
authors.forename: Insik
authors.initials: I
authors.lastname: In
authors.affiliation: Department of IT Convergence, Korea National University of Transportation, Chungju 380-702, Republic of Korea; Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 380-702, Republic of Korea. Electronic address: parkchem@ut.ac.kr.
authors.forename: Sung Young
authors.initials: SY
authors.lastname: Park
mesh_terms: D001896:Boron Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001897:Boronic Acids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006108:Graphite / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008247:Lysosomes / Q000378:metabolism* / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry*; D009369:Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D001897:Boronic Acids; D005456:Fluorescent Dyes; D006108:Graphite
keywords: Catechol chemistry; Fluorescent nanomaterial; pH- and thermo-responsive