title: Structural and elemental changes in glioblastoma cells in situ: complementary imaging with high resolution visible light- and X-ray microscopy.
abstract: The glioblastoma (GBM) is characterized by a short median survival and an almost 100% tumor related mortality. GBM cells exhibit highly invasive behavior whose mechanisms are not yet fully understood. The present study explores application of X-ray and visible light microscopy to display the elemental and structural images of cells from 3 patient derived GMB samples and an established GMB cell line. Slight differences in elemental concentrations, in actin cytoskeleton organization and cell morphology were noted between all cells types by X-ray fluorescence and full field soft X-ray microscopy, as well as the Structured Illumination Super-resolution Microscope (SIM). Different sample preparation approaches were used to match each imaging technique. While preparation for SIM included cell fixation and staining, intact frozen hydrated cells were used for the trace element imaging by hard X-ray fluorescence and exploration of the structural features by soft X-ray absorption tomography. Each technique documented differences between samples with regard to morphology and elemental composition and underscored the importance of use of multiple patient derived samples for detailed GBM study.
authors.affiliation: CELLS - ALBA, Carrer de la Llum 2-26, 08290 Cerdanyola del Vallès, Barcelona, Spain. tducic@cells.es.
authors.forename: Tanja
authors.initials: T
authors.lastname: Dučić
authors.affiliation: Department of Radiation Oncology, Northwestern University, 300 E. Superior St, Chicago, IL 60611, USA.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Paunesku
authors.affiliation: Advanced Photon Source, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA.
authors.forename: Si
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Neurosurgery, Georg-August University Medical Centre, 37075 Göttingen, Germany.
authors.forename: Milena
authors.initials: M
authors.lastname: Ninković
authors.affiliation: Department of Neurosurgery, Georg-August University Medical Centre, 37075 Göttingen, Germany.
authors.forename: Swetlana
authors.initials: S
authors.lastname: Speling
authors.affiliation: Northwestern University, Biological Imaging Facility, 2205 Tech Drive, Evanston, IL 60208, USA.
authors.forename: Charlene
authors.initials: C
authors.lastname: Wilke
authors.affiliation: Advanced Photon Source, Argonne National Laboratory, 9700 S. Cass Avenue, Argonne, IL 60439, USA.
authors.forename: Barry
authors.initials: B
authors.lastname: Lai
authors.affiliation: Department of Radiation Oncology, Northwestern University, 300 E. Superior St, Chicago, IL 60611, USA.
authors.forename: Gayle
authors.initials: G
authors.lastname: Woloschak
mesh_terms: D000199:Actins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D005909:Glioblastoma / Q000473:pathology* / Q000648:ultrastructure; D006801:Humans; D019216:Metals, Heavy / Q000378:metabolism; D008853:Microscopy; D013052:Spectrometry, X-Ray Emission; D014056:Tomography, X-Ray
chemical_list: D000199:Actins; D019216:Metals, Heavy