title: Naturally occurring high oleic acid cottonseed oil: identification and functional analysis of a mutant allele of Gossypium barbadense fatty acid desaturase-2.
abstract: Some naturally occurring cotton accessions contain commercially attractive seed oil fatty acid profiles. The likely causal factor for a high-oleate trait in pima cotton ( Gossypium barbadense ) accession GB-713 is described here. Vegetable oils are broadly used in the manufacture of many human and animal nutritional products, and in various industrial applications. Along with other well-known edible plant oils from soybean, corn, and canola, cottonseed oil is a valuable commodity. Cottonseed oil is a co-product derived from the processing of cottonseed fiber. In the past, it was used extensively in a variety of food applications. However, cottonseed oil has lost market share in recent years due to less than optimal ratios of the constituent fatty acids found in either traditional or partially hydrogenated oil. Increased awareness of the negative health consequences of dietary trans-fats, along with the public wariness associated with genetically modified organisms has created high demand for naturally occurring oil with high monounsaturate/polyunsaturate ratios. Here, we report the discovery of multiple exotic accessions of pima cotton that contain elevated seed oil oleate content. The genome of one such accession was sequenced, and a mutant candidate fatty acid desaturase-2 (FAD2-1D) gene was identified. The mutant protein produced significantly less linoleic acid in infiltrated Arabidopsis leaf assays, compared to a repaired version of the same enzyme. Identification of this gene provides a valuable resource. Development of markers associated with this mutant locus will be very useful in efforts to breed the high-oleate trait into agronomic fiber accessions of upland cotton.
authors.affiliation: Commodity Utilization Research Unit, United States Department of Agriculture-Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, USA. Jay.Shockey@ars.usda.gov.
authors.forename: Jay
authors.identifier: http://orcid.org/0000-0002-5057-5457
authors.initials: J
authors.lastname: Shockey
authors.affiliation: Commodity Utilization Research Unit, United States Department of Agriculture-Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Dowd
authors.affiliation: Food and Feed Safety Research Unit, United States Department of Agriculture-Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Mack
authors.affiliation: Food and Feed Safety Research Unit, United States Department of Agriculture-Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, USA.
authors.forename: Matthew
authors.initials: M
authors.lastname: Gilbert
authors.affiliation: Genomics and Bioinformatics Research Unit, United States Department of Agriculture-Agricultural Research Service, Stoneville, MS, USA.
authors.forename: Brian
authors.initials: B
authors.lastname: Scheffler
authors.affiliation: Genomics and Bioinformatics Research Unit, United States Department of Agriculture-Agricultural Research Service, Stoneville, MS, USA.
authors.forename: Linda
authors.initials: L
authors.lastname: Ballard
authors.affiliation: Crop Germplasm Research Unit, United States Department of Agriculture-Agricultural Research Service, College Station, TX, USA.
authors.forename: James
authors.initials: J
authors.lastname: Frelichowski
authors.affiliation: Commodity Utilization Research Unit, United States Department of Agriculture-Agricultural Research Service, Southern Regional Research Center, New Orleans, LA, USA.
authors.forename: Catherine
authors.initials: C
authors.lastname: Mason
mesh_terms: D000483:Alleles; D000595:Amino Acid Sequence; D002849:Chromatography, Gas; D003369:Cottonseed Oil / Q000737:chemistry*; D044943:Fatty Acid Desaturases / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D003368:Gossypium / Q000201:enzymology* / Q000235:genetics; D019787:Linoleic Acid / Q000032:analysis; D009154:Mutation / Q000235:genetics*; D019301:Oleic Acid / Q000378:metabolism*; D010802:Phylogeny; D020871:RNA Stability / Q000235:genetics; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA
chemical_list: D003369:Cottonseed Oil; D012333:RNA, Messenger; D019301:Oleic Acid; D019787:Linoleic Acid; D044943:Fatty Acid Desaturases
keywords: Cottonseed; Fatty acid desaturase; Linoleic acid; Oleic acid