title: Polyoxometalates as artificial nucleases: hydrolytic cleavage of DNA promoted by a highly negatively charged ZrIV-substituted Keggin polyanion.
abstract: A highly negatively charged binuclear ZrIV-substituted Keggin polyoxometalate [{α-PW11O39Zr(μ-OH)(H2O)}2]8-(ZrK 2 : 2) has been shown to promote the hydrolytic cleavage of phosphoester bonds in the supercoiled plasmid pUC19 DNA under physiological pH and temperature, giving relaxed and linear forms of pUC19 as hydrolysis products. The interaction between ZrK 2 : 2 and DNA was experimentally proven by circular dichroism (CD) spectroscopy and31P diffusion ordered NMR spectroscopy.
authors.affiliation: Department of Chemistry, KU Leuven, Celestijnenlaan 200F and 200G, 3001 Heverlee, Belgium. Tatjana.Vogt@kuleuven.be.
authors.forename: T K N
authors.initials: TK
authors.lastname: Luong
authors.affiliation: Department of Chemistry, KU Leuven, Celestijnenlaan 200F and 200G, 3001 Heverlee, Belgium. Tatjana.Vogt@kuleuven.be.
authors.forename: I
authors.initials: I
authors.lastname: Govaerts
authors.affiliation: Department of Chemistry, KU Leuven, Celestijnenlaan 200F and 200G, 3001 Heverlee, Belgium. Tatjana.Vogt@kuleuven.be.
authors.forename: J
authors.initials: J
authors.lastname: Robben
authors.affiliation: Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Acad. G. Bontchev Street, Bl.9, 1113 Sofia, Bulgaria.
authors.forename: P
authors.initials: P
authors.lastname: Shestakova
authors.affiliation: Department of Chemistry, KU Leuven, Celestijnenlaan 200F and 200G, 3001 Heverlee, Belgium. Tatjana.Vogt@kuleuven.be.
authors.forename: T N
authors.identifier: 0000-0002-6188-3957
authors.initials: TN
authors.lastname: Parac-Vogt
mesh_terms: D053837:DNA Cleavage; D004278:DNA, Superhelical / Q000737:chemistry*; D006868:Hydrolysis; D000071228:Polyelectrolytes; D011108:Polymers / Q000737:chemistry; D017973:Tungsten Compounds / Q000737:chemistry*; D015040:Zirconium / Q000737:chemistry*; D000099294:Polyoxometalates
chemical_list: D004278:DNA, Superhelical; D000071228:Polyelectrolytes; D011108:Polymers; D017973:Tungsten Compounds; D015040:Zirconium; C102296:polyoxometalate I; D000099294:Polyoxometalates