title: Interaction of Prussian blue nanoparticles with bovine serum albumin: a multi-spectroscopic approach.
abstract: Owning to their exceptional properties, Prussian blue nanoparticles (PBNPs) are promising in a variety of biomedical applications. In this scenario, understanding of how PBNPs interact and behave in biological systems is essential. Herein, the interaction of PBNPs with protein was investigated. Specifically, the citric acid stabilized PBNPs with a size of 10 nm were synthesized and characterized. The interactions of these PBNPs with the model protein, bovine serum albumin (BSA), were then probed by spectroscopic methods. It was found that the BSA intrinsic fluorescence was quenched upon addition of PBNPs due to the static interaction, suggesting the binding of PBNPs with BSA. Moreover, the synchronous fluorescence and circular dichroism spectra indicated the conformational change of BSA due to the presence of PBNPs.
authors.affiliation: a School of Basic Medical Sciences , Capital Medical University , Beijing 100069 , P.R. China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Zhou
authors.affiliation: b School of Chemical Biology and Pharmaceutical Sciences , Capital Medical University , Beijing 100069 , P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Shi
authors.affiliation: c Medical Experiment and Test Center , Capital Medical University , Beijing 100069 , P.R. China.
authors.forename: Yuanjie
authors.initials: Y
authors.lastname: Fan
authors.affiliation: a School of Basic Medical Sciences , Capital Medical University , Beijing 100069 , P.R. China.
authors.forename: Zhiying
authors.initials: Z
authors.lastname: He
authors.affiliation: b School of Chemical Biology and Pharmaceutical Sciences , Capital Medical University , Beijing 100069 , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Gu
authors.affiliation: b School of Chemical Biology and Pharmaceutical Sciences , Capital Medical University , Beijing 100069 , P.R. China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ye
authors.affiliation: d Beijing Neurosurgical Institute , Capital Medical University , Beijing 100069 , P.R. China.
authors.forename: Fangang
authors.initials: F
authors.lastname: Meng
mesh_terms: D000465:Algorithms; D000818:Animals; D001665:Binding Sites; D002417:Cattle; D002942:Circular Dichroism; D005295:Ferrocyanides / Q000737:chemistry* / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D011485:Protein Binding; D012710:Serum Albumin, Bovine / Q000737:chemistry* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013816:Thermodynamics; D014961:X-Ray Diffraction
chemical_list: D005295:Ferrocyanides; D012710:Serum Albumin, Bovine; C000170:ferric ferrocyanide
keywords: Prussian blue nanoparticals (PBNPs); bovine serum albumin (BSA); circular dichroism (CD); fluorescence quenching; interaction