title: Nesfatin-1 influences the excitability of gastric distension-responsive neurons in the ventromedial hypothalamic nucleus of rats.
abstract: The present study investigated the effects of nesfatin-1 on gastric distension (GD)-responsive neurons via an interaction with corticotropin-releasing factor (CRF) receptor signaling in the ventromedial hypothalamic nucleus (VMH), and the potential regulation of these effects by hippocampal projections to VMH. Extracellular single-unit discharges were recorded in VHM following administration of nesfatin-1. The projection of nerve fibers and expression of nesfatin-1 were assessed by retrograde tracing and fluoro-immunohistochemical staining, respectively. Results showed that there were GD-responsive neurons in VMH; Nesfatin-1 administration and electrical stimulation of hippocampal CA1 sub-region altered the firing rate of these neurons. These changes could be partially blocked by pretreatment with the non-selective CRF antagonist astressin-B or an antibody to NUCB2/nesfatin-1. Electrolytic lesion of CA1 hippocampus reduced the effects of nesfatin-1 on VMH GD-responsive neuronal activity. These studies suggest that nesfatin-1 plays an important role in GD-responsive neuronal activity through interactions with CRF signaling pathways in VMH. The hippocampus may participate in the modulation of nesfatin-1-mediated effects in VMH.
authors.affiliation: Department of Pathophysiology, Medical College of Qingdao University, Qingdao, Shandong, PR China. xu.luo@163.com.
authors.forename: Hongzhen
authors.initials: H
authors.lastname: Feng
authors.forename: Qiaoling
authors.initials: Q
authors.lastname: Wang
authors.forename: Feifei
authors.initials: F
authors.lastname: Guo
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Han
authors.forename: Mingjie
authors.initials: M
authors.lastname: Pang
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Sun
authors.forename: Yanling
authors.initials: Y
authors.lastname: Gong
authors.forename: Luo
authors.initials: L
authors.lastname: Xu
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism*; D003346:Corticotropin-Releasing Hormone / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D005769:Gastrointestinal Motility / Q000502:physiology*; D008297:Male; D009419:Nerve Tissue Proteins / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D000080866:Nucleobindins; D051381:Rats; D017208:Rats, Wistar; D013270:Stomach / Q000502:physiology*; D014697:Ventromedial Hypothalamic Nucleus / Q000502:physiology*
chemical_list: D002135:Calcium-Binding Proteins; D004268:DNA-Binding Proteins; D009419:Nerve Tissue Proteins; C000630652:Nucb1 protein, rat; D000080866:Nucleobindins; D003346:Corticotropin-Releasing Hormone