title: Genome-Wide Association Study for Plant Height and Grain Yield in Rice under Contrasting Moisture Regimes.
abstract: Drought is one of the vitally critical environmental stresses affecting both growth and yield potential in rice. Drought resistance is a complicated quantitative trait that is regulated by numerous small effect loci and hundreds of genes controlling various morphological and physiological responses to drought. For this study, 270 rice landraces and cultivars were analyzed for their drought resistance. This was done via determination of changes in plant height and grain yield under contrasting water regimes, followed by detailed identification of the underlying genetic architecture via genome-wide association study (GWAS). We controlled population structure by setting top two eigenvectors and combining kinship matrix for GWAS in this study. Eighteen, five, and six associated loci were identified for plant height, grain yield per plant, and drought resistant coefficient, respectively. Nine known functional genes were identified, including five for plant height (OsGA2ox3, OsGH3-2, sd-1, OsGNA1, andOsSAP11/OsDOG), two for grain yield per plant (OsCYP51G3andOsRRMh) and two for drought resistant coefficient (OsPYL2andOsGA2ox9), implying very reliable results. A previous study reportedOsGNA1to regulate root development, but this study reports additional controlling of both plant height and root length. Moreover,OsRLK5is a new drought resistant candidate gene discovered in this study.OsRLK5mutants showed faster water loss rates in detached leaves. This gene plays an important role in the positive regulation of yield-related traits under drought conditions. We furthermore discovered several new loci contributing to the three investigated traits (plant height, grain yield, and drought resistance). These associated loci and candidate genes significantly improve our knowledge of the genetic control of these traits in rice. In addition, many drought resistant cultivars screened in this study can be used as parental genotypes to improve drought resistance of rice by molecular breeding.
authors.affiliation: College of Plant Sciences & Technology, Huazhong Agricultural UniversityWuhan, China; Shanghai Agrobiological Gene CenterShanghai, China.
authors.forename: Xiaosong
authors.initials: X
authors.lastname: Ma
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Feng
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Haibin
authors.initials: H
authors.lastname: Wei
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Hanwei
authors.initials: H
authors.lastname: Mei
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Xu
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Shoujun
authors.initials: S
authors.lastname: Chen
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Tianfei
authors.initials: T
authors.lastname: Li
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Liang
authors.affiliation: Shanghai Agrobiological Gene Center Shanghai, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Plant Sciences & Technology, Huazhong Agricultural UniversityWuhan, China; Shanghai Agrobiological Gene CenterShanghai, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Luo
keywords: GWAS; candidate genes; drought resistance; grain yield; plant height