title: Biocompatibility and drug release behavior of curcumin conjugated gold nanoparticles from aminosilane-functionalized electrospun poly(N-vinyl-2-pyrrolidone) fibers.
abstract: Curcumin (CUR) has a wide spectrum of biological and pharmacological activities, yet problems of its bioavailability remained a major challenge in preclinical studies. Thus, the design of the delivery systems with CUR as a model drug featuring dual release process - an initial burst followed by sustained release - to provide the optimal drug pharmacokinetics in the therapeutic region has been actively pursued. In this study, the 3-aminopropyltriethoxysilane (APTES)-functionalized electrospun poly(N-vinyl-2-pyrrolidone) fibers (NH2-PVP) were utilized as a free-standing substrate for the immobilization of CUR-PVP capped gold nanoparticles (CUR-PGNPs) conjugates. The conjugate was synthesized by sonication and the drug entrapment percentage was determined to be 54.2 ±1.8. CUR-PGNPs immobilized on NH2-PVP fibers showed a moderate burst release during the first few hours, followed by a sustained release lasting for 2days. The drug release was found pH-dependent (pH 5.0>6.0>7.4). The two-stage release profiles of CUR-PGNPs@NH2-PVP fibers were fitted well to Korsmeyer-Peppas model, indicating a non-Fickian diffusion mechanism for initial burst release and Fickian diffusion-controlled mechanism for the sustained release. Initial biocompatibility assessments based on lactate dehydrogenase (LDH) assay and morphological examination by SEM with L-929 mouse fibroblasts revealed that CUR-PGNPs@NH2-PVP nanofibrous scaffold was capable of supporting cell growth over a culture period of 3days.
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 106, Taiwan.
authors.forename: Alfin
authors.initials: A
authors.lastname: Kurniawan
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 106, Taiwan.
authors.forename: Farrel
authors.initials: F
authors.lastname: Gunawan
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 106, Taiwan.
authors.forename: Adi Tama
authors.initials: AT
authors.lastname: Nugraha
authors.affiliation: Department of Chemical Engineering, Widya Mandala Surabaya Catholic University, Kalijudan 37, Surabaya 60114, Indonesia.
authors.forename: Suryadi
authors.initials: S
authors.lastname: Ismadji
authors.affiliation: Department of Chemical Engineering, National Taiwan University of Science and Technology, No. 43, Sec. 4, Keelung Rd., Taipei 106, Taiwan. Electronic address: mjwang@mail.ntust.edu.tw.
authors.forename: Meng-Jiy
authors.initials: MJ
authors.lastname: Wang
mesh_terms: D000818:Animals; D002460:Cell Line; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003474:Curcumin / Q000008:administration & dosage* / Q000737:chemistry; D003692:Delayed-Action Preparations; D016503:Drug Delivery Systems; D065546:Drug Liberation; D005347:Fibroblasts / Q000378:metabolism; D006046:Gold; D006863:Hydrogen-Ion Concentration; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D053768:Metal Nanoparticles; D051379:Mice; D011145:Polyvinyls / Q000737:chemistry; D011437:Propylamines / Q000737:chemistry*; D011759:Pyrrolidines / Q000737:chemistry; D012821:Silanes / Q000737:chemistry*; D013997:Time Factors
chemical_list: D003692:Delayed-Action Preparations; D011145:Polyvinyls; D011437:Propylamines; D011759:Pyrrolidines; D012821:Silanes; C505150:poly(N-vinylpyrrolidine); D006046:Gold; D007770:L-Lactate Dehydrogenase; D003474:Curcumin; C477625:amino-propyl-triethoxysilane
keywords: Aminosilane functionalization; Biocompatibility; Curcumin; Gold nanoparticles; Sustained release