title: Long Noncoding RNA Highly Up-regulated in Liver Cancer Predicts Unfavorable Outcome and Regulates Metastasis by MMPs in Triple-negative Breast Cancer.
abstract: OBJECTIVE
  Long noncoding RNA (lncRNA) highly up-regulated in liver cancer (HULC) is the first identified lncRNA highly expressed in hepatocellular carcinoma and acts as an oncogenic lncRNA. However, the significance of HULC in triple-negative breast cancer (TNBC) is still unclear. Our purpose is to explore the clinical significance and biological function of HULC in TNBC.
  
  
  METHODS
  Expression of HULC in TNBC tissues and cell lines was measured and the association between HULC expression and clinicopathological factors was analyzed. Cell proliferation, migration, and invasion assays were executed by using TNBC cell lines transfected with si-HULC or si-NC. The potential mechanism of HULC was explored by Western blot.
  
  
  RESULTS
  HULC expression was increased in TNBC tissues and cell lines and associated with malignant status and poor prognosis of TNBC patients. Furthermore, silencing TNBC expression effectively suppressed TNBC cells metastasis through MMP-2 and MMP-9.
  
  
  CONCLUSIONS
  HULC acts as an oncogene lncRNA in TNBC and as an independent poor prognostic factor in TNBC patients.
authors.affiliation: Internal Medicine Department of Thyroid and Radionuclide Therapy, Hunan Cancer Hospital, The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Shi
authors.affiliation: Department of Health Toxicology, Xiangya School of Public Health, Central South University, Changsha, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xiao
authors.affiliation: Center of Preventive Medicine Experiment, Xiangya School of Public Health, Central South University, Changsha, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Ding
authors.affiliation: Department of Nutrition and Food Hygiene, Xiangya School of Public Health, Central South University, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Occupational and Environmental Health, Xiangya School of Public Health, Central South University, China. Electronic address: huangruixue_ss@163.com.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Huang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism* / Q000473:pathology; D020782:Matrix Metalloproteinases / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D064726:Triple Negative Breast Neoplasms / Q000378:metabolism* / Q000473:pathology; D015854:Up-Regulation
chemical_list: C574685:HULC long non-coding RNA, human; D062085:RNA, Long Noncoding; D020782:Matrix Metalloproteinases
keywords: HULC; MMP; Metastasis; Triple negative breast cancer; lncRNA