title: LncRNA-ANCR down-regulation suppresses invasion and migration of colorectal cancer cells by regulating EZH2 expression.
abstract: Our study aimed to explore the effects of long noncoding RNA (lncRNA)-ANCR on the invasion and migration of colorectal cancer (CRC) cells by regulating enhancer of zeste homolog 2 (EZH2) expression. CRC tissues and adjacent normal tissues were collected and CRC SW620 cells line and normal human intestinal epithelial cells (HIECs) were incubated. CRC SW620 cells line was transfected with ANCR-siRNA. The expressions of ANCR and EZH2 mRNA were measured by real-time quantitative polymerase chain reaction (RT-qPCR). EZH2 and trimethylation of H3K27 (H3K27me3) protein expressions were detected using Western blotting. The relationship between ANCR and EZH2 was determined through RNA pull-down and co-immunoprecipitation (co-IP) assays. Cell invasion and migration were determined by Trans-well and cell scratch assays. ANCR, EZH2 and H3K27me3 expressions were up-regulated in CRC tissues and SW620 cells (all P < 0.05). After transfected with ANCR-siRNA, SW620 cells showed decreased ANCR expression and EZH2 mRNA and protein expressions (all P < 0.05). According to the results of RNA pull-down and co-IP assays, ANCR could specifically bind to EZH2. The results of Trans-well and cell scratch tests showed that when ANCR expression was decreased, the invasion and migration abilities of SW620 cells significantly declined (both P < 0.05). In conclusion, these results suggest that lncRNA-ANCR could influence the invasion and migration of CRC cells by specifically binding to EZH2.
authors.affiliation: Department of First Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Yang
authors.affiliation: Department of First Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yang
authors.affiliation: Department of Internal Medicine, Harbin Red Cross Central Hospital, Harbin, Heilongjiang, China.
authors.forename: Ying-Li
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of First Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
authors.forename: Bao
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of First Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of First Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Hong
authors.affiliation: Department of Sixth Internal Medicine, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Department of Internal Medicine, The First Hospital of Harbin, Harbin, Heilongjiang, China.
authors.forename: Yao-Hui
authors.initials: YH
authors.lastname: Zhou
authors.affiliation: Department of Internal Medicine, The First Hospital of Harbin, Harbin, Heilongjiang, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Zeng
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000473:pathology*; D015536:Down-Regulation; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000235:genetics*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D062085:RNA, Long Noncoding / Q000096:biosynthesis / Q000235:genetics*; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics
chemical_list: C571057:ANCR long noncoding RNA, human; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; C498409:EZH2 protein, human; D000071221:Enhancer of Zeste Homolog 2 Protein
keywords: ANCR; EZH2; LncRNA; colorectal cancer; invasion; migration