title: RNA interference in mosquito: understanding immune responses, double-stranded RNA delivery systems and potential applications in vector control.
abstract: RNA interference (RNAi) refers to the process of post-transcriptional silencing of cellular mRNA by the application of double-stranded RNA (dsRNA). RNAi strategies have been widely employed to regulate gene expression in plants and animals including insects. With the availability of the full genome sequences of major vector mosquitoes, RNAi has been increasingly used to conduct genetic studies of human pathogens in mosquito vectors and to study the evolution of insecticide resistance in mosquitoes. This review summarizes the recent progress in our understanding of mosquito-pathogen interactions using RNAi and various methods of dsRNA delivery in mosquitoes at different stages. We also discuss potential applications of this technology to develop novel tools for vector control.
authors.affiliation: Central Inter-Disciplinary Research Facility, Sri Balaji Vidyapeeth University, Puducherry, India.
authors.forename: A
authors.initials: A
authors.lastname: Balakrishna Pillai
authors.affiliation: SASTRA University, Thanjavur, India.
authors.forename: U
authors.initials: U
authors.lastname: Nagarajan
authors.affiliation: Department of Microbiology, Adamas University, Kolkata, India.
authors.forename: A
authors.initials: A
authors.lastname: Mitra
authors.affiliation: SASTRA University, Thanjavur, India.
authors.forename: U
authors.initials: U
authors.lastname: Krishnan
authors.affiliation: Jawaharlal Institute for Post Medical Education and Research, Puducherry, India.
authors.forename: S
authors.initials: S
authors.lastname: Rajendran
authors.affiliation: Regional Medical Research Centre, ICMR, Belgaum, India.
authors.forename: S L
authors.initials: SL
authors.lastname: Hoti
authors.affiliation: Centre for Cellular and Molecular Biology, Hyderabad, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Mishra
mesh_terms: D000818:Animals; D009032:Mosquito Control; D036103:Nanotechnology; D034622:RNA Interference; D012330:RNA, Double-Stranded / Q000008:administration & dosage*
chemical_list: D012330:RNA, Double-Stranded
keywords: dsRNA delivery systems in mosquitoes; nanotechnology and vector control; systemic RNAi response