title: Sex difference in the contribution of GABABreceptors to tibial neuromodulation of bladder overactivity in cats.
abstract: This study investigated the role of γ-aminobutyric acid subtype B (GABAB) receptors in tibial and pudendal neuromodulation of bladder overactivity induced by intravesical administration of dilute (0.5%) acetic acid (AA) in α-chloralose-anesthetized cats. To inhibit bladder overactivity, tibial or pudendal nerve stimulation (TNS or PNS) was applied at 5 Hz and two or four times threshold (T) intensity for inducing toe or anal sphincter twitch. TNS at 2T or 4T intensity significantly (P< 0.05) increased the bladder capacity to 173.8 ± 16.2 or 198.5 ± 24.1%, respectively, of control capacity. Meanwhile, PNS at 2T or 4T intensity significantly (P< 0.05) increased the bladder capacity to 217 ± 18.8 and 221.3 ± 22.3% of control capacity, respectively. CGP52432 (a GABABreceptor antagonist) at intravenous dosages of 0.1-1 mg/kg completely removed the TNS inhibition in female cats but had no effect in male cats. CGP52432 administered intravenously also had no effect on control bladder capacity or the pudendal inhibition of bladder overactivity. These results reveal a sex difference in the role of GABABreceptors in tibial neuromodulation of bladder overactivity in cats and that GABABreceptors are not involved in either pudendal neuromodulation or irritation-induced bladder overactivity.
authors.affiliation: Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Thomas W
authors.initials: TW
authors.lastname: Fuller
authors.affiliation: Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania.|Department of Urology, Qilu Hospital, Shandong University, Jinan, Peoples Republic of China; and.
authors.forename: Xuewen
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Utsav
authors.initials: U
authors.lastname: Bansal
authors.affiliation: Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Lamm
authors.affiliation: Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Bing
authors.initials: B
authors.lastname: Shen
authors.affiliation: Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Jicheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: James R
authors.initials: JR
authors.lastname: Roppolo
authors.affiliation: Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: William C
authors.initials: WC
authors.lastname: de Groat
authors.affiliation: Department of Urology, University of Pittsburgh, Pittsburgh, Pennsylvania; cftai@pitt.edu.|Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, Pennsylvania.
authors.forename: Changfeng
authors.initials: C
authors.lastname: Tai
mesh_terms: D000818:Animals; D002415:Cats; D004599:Electric Stimulation Therapy / Q000379:methods*; D005260:Female; D008297:Male; D060525:Pudendal Nerve / Q000502:physiology; D018080:Receptors, GABA-B / Q000378:metabolism*; D017981:Receptors, Neurotransmitter / Q000378:metabolism; D012727:Sex Characteristics; D013979:Tibial Nerve / Q000503:physiopathology*; D016896:Treatment Outcome; D001743:Urinary Bladder / Q000294:innervation / Q000503:physiopathology*; D053201:Urinary Bladder, Overactive / Q000503:physiopathology* / Q000517:prevention & control*
chemical_list: D018080:Receptors, GABA-B; D017981:Receptors, Neurotransmitter
keywords: GABA; bladder; cat; neuromodulation