title: Comparative assessment of phytochemical profiles, antioxidant and antiproliferative activities of Sea buckthorn (Hippophaë rhamnoides L.) berries.
abstract: Phytochemical profiles, antioxidant and antiproliferative activities of berry extracts were evaluated and compared in four subspecies of Sea buckthorn (Hippophaë rhamnoides L.). Among the subspecies, Hippophaë rhamnoides L. subsp. sinensis exhibited highest total phenolics content (38.7±1.3mgGA equiv./g DW) and corresponding total antioxidant activity. Whereas maximum cellular antioxidant and antiproliferative activities were determined in Hippophaë rhamnoides L. subsp. yunnanensis. Total antioxidant activity was significantly associated to total phenolics, isorhamnetin-3-rutinoside and isorhamnetin-3-glucoside. The cellular antioxidant activity and antiproliferative activity of phytochemicals were fairly correlated to phenolic acids and flavonoid aglycones. Lower median effective dose (EC50) of individual compounds against human liver cancer HepG2 cells proliferation studies confirmed the better correlation between antiproliferative activity of Sea buckthorn extracts and flavonoid aglycones, including isorhamnetin, quercetin and kaempferol.
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Ruixue
authors.initials: R
authors.lastname: Guo
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Department of Food Science, Cornell University, Ithaca, NY 14853, United States. Electronic address: xbg720@gmail.com.
authors.forename: Xinbo
authors.initials: X
authors.lastname: Guo
authors.affiliation: Department of Food Science, Cornell University, Ithaca, NY 14853, United States.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Food Science, Cornell University, Ithaca, NY 14853, United States. Electronic address: rl23@cornell.edu.
authors.forename: Rui Hai
authors.initials: RH
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D005638:Fruit / Q000737:chemistry*; D031281:Hippophae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D000975:Antioxidants; D005419:Flavonoids; D010636:Phenols; D064209:Phytochemicals; D010936:Plant Extracts; C047368:3-methylquercetin; D011794:Quercetin
keywords: Antioxidant activity; Antiproliferative activity; Phenolics; Phytochemicals; Sea buckthorn