title: Plasma Intermedin Level Indicates Severity and Treatment Efficacy of Septic Shock in Sprague-Dawley (SD) Rats.
abstract: BACKGROUND The aim of this study was to investigate the value of plasma intermedin (IMD) in assessing severity and treatment efficacy of septic shock. MATERIAL AND METHODS Healthy male Sprague-Dawley (SD) rats were chosen and divided into a normal control group (n=15) and a shock model group (n=27) that received intravenous injection of lipopolysaccharide (LPS). Then, 3 specimens were taken from each group. The shock model group rats were divided into an LPS group and a treatment group with 12 rats each. The treatment group received intravenous injection of compound sodium lactate solution. Plasma IMD and IMD1-47 mRNA expressions were compared and analyzed. RESULTS Mean arterial pressure (MAP) was lower while white blood cell count and TNF-α were higher in the shock model group than in the normal control group (P<0.05). After 10 h and 20 h, the treatment group had lower plasma IMD and IMD1-47 mRNA expressions compared with the LPS group (P<0.05). Plasma IMD and IMD1-47 mRNA expressions in the LPS group after 20 h were significantly higher than after 10 h (P<0.05). IMD was positively correlated with interleukins (IL-3, IL-6, and IL-8), white blood cell count, and body temperature (all P<0.05), but were negatively correlated with systolic pressure (r=-0.8474, P=0.0040). CONCLUSIONS Plasma IMD level can effectively reflect the severity of septic shock and can be used as an important indicator of septic shock treatment effectiveness.
authors.affiliation: Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China (mainland).
authors.forename: Su-Xian
authors.initials: SX
authors.lastname: Yang
authors.affiliation: Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China (mainland).
authors.forename: Yun-Xiu
authors.initials: YX
authors.lastname: Chen
authors.affiliation: Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China (mainland).
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Infectious Diseases, Linyi People's Hospital, Linyi, Shandong, China (mainland).
authors.forename: Zhao-Hui
authors.initials: ZH
authors.lastname: Yang
mesh_terms: D053607:Adrenomedullin / Q000097:blood* / Q000235:genetics; D000818:Animals; D054316:Biomarkers, Pharmacological / Q000097:blood; D007275:Injections, Intravenous; D007378:Interleukins / Q000097:blood; D008070:Lipopolysaccharides / Q000008:administration & dosage; D008297:Male; D009479:Neuropeptides / Q000097:blood* / Q000235:genetics; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D012772:Shock, Septic / Q000097:blood* / Q000139:chemically induced / Q000188:drug therapy*; D019354:Sodium Lactate / Q000008:administration & dosage; D016896:Treatment Outcome
chemical_list: C499411:Adm2 protein, rat; D054316:Biomarkers, Pharmacological; D007378:Interleukins; D008070:Lipopolysaccharides; D009479:Neuropeptides; D012333:RNA, Messenger; D053607:Adrenomedullin; D019354:Sodium Lactate