title: Intracytoplasmic Transport of Hepatitis B Virus Capsids.
abstract: The early steps of HBV entry remain largely unknown despite the recent discovery of an HBV-specific entry receptor. Following entry HBV capsids have to be transported through the cytoplasm to the nuclear periphery, followed by nuclear entry. These steps have to take place in a coordinated manner to allow delivery of the genome into the nucleus. Due to the viscosity of the cytoplasm, the intracytoplasmic translocation has to be active and directed.Here, we describe protocols that can be applied to investigations of the HBV capsid with the cytoplasmic transport systems. We have chosen to present two independent experimental approaches, which allow avoiding artifacts. Aside of the specific capsid detection system, the protocols can be applied to any other viral structure.
authors.affiliation: University of Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France.|CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France.
authors.forename: Quentin
authors.initials: Q
authors.lastname: Osseman
authors.affiliation: University of Bordeaux, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France. michael.kann@u-bordeaux.fr.|CNRS, Microbiologie Fondamentale et Pathogénicité, UMR 5234, Bordeaux, France. michael.kann@u-bordeaux.fr.|Centre Hospitalier Universitaire de Bordeaux, Service de Virologie, Bordeaux, France. michael.kann@u-bordeaux.fr.
authors.forename: Michael
authors.initials: M
authors.lastname: Kann
mesh_terms: D000818:Animals; D002213:Capsid / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism; D004398:Dyneins / Q000378:metabolism; D006509:Hepatitis B / Q000378:metabolism / Q000821:virology*; D006515:Hepatitis B virus / Q000502:physiology*; D006801:Humans; D008870:Microtubules / Q000737:chemistry / Q000378:metabolism; D009865:Oocytes; D011485:Protein Binding; D055503:Protein Multimerization; D021381:Protein Transport; D014982:Xenopus laevis
chemical_list: D004398:Dyneins
keywords: Capsids; Dynein; Hepatitis B virus; Microtubules; Retrograde transport