title: MiR-151a is involved in the pathogenesis of atopic dermatitis by regulating interleukin-12 receptor β2.
abstract: MicroRNAs (miRNAs) have been reported to circulate in the blood in a highly stable and cell-free form. Dysregulated expression of miRNAs has been detected in various pathological conditions including atopic dermatitis. In our study, human blood plasma miRNAs were identified by high-throughput sequencing and compared among patients of atopic dermatitis and healthy controls. We found that miR-151a was differentially expressed in the plasma of atopic dermatitis patients. MiR-151a regulates the expression of IL12RB2 by targeting two loci in the 3' untranslated region of the Il12rb2 gene. Moreover, IL12RB2 was remarkably downregulated in Jurkat cells overexpressing miR-151a. Jurkat cells treated with phytohemagglutinin also showed reduced expression of IFN-γ, interleukin-2 (IL-2) and IL-12. Together, these results suggest that miR-151a is involved in the pathogenesis of atopic dermatitis by regulating IL12RB2.
authors.affiliation: Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.
authors.forename: Xiao-Fan
authors.initials: XF
authors.lastname: Chen
authors.affiliation: Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Dou
authors.affiliation: Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Shao
authors.affiliation: Shenzhen Key Laboratory for Drug Addiction and Safety Application, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.
authors.forename: Xiao-Jian
authors.initials: XJ
authors.lastname: Jia
authors.affiliation: Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Shenzhen Key Laboratory for Translational Medicine of Dermatology, Biomedical Research Institute, Shenzhen Peking University - The Hong Kong University of Science and Technology Medical Center, Shenzhen, Guangdong Province, China.|Department of Dermatology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Yu
mesh_terms: D020413:3' Untranslated Regions; D000293:Adolescent; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D003876:Dermatitis, Atopic / Q000097:blood* / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D005786:Gene Expression Regulation / Q000235:genetics*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007371:Interferon-gamma / Q000235:genetics; D018664:Interleukin-12 / Q000235:genetics; D053712:Interleukin-12 Receptor beta 2 Subunit / Q000235:genetics*; D007376:Interleukin-2 / Q000235:genetics; D019169:Jurkat Cells; D008297:Male; D010835:Phytohemagglutinins / Q000494:pharmacology; D055815:Young Adult
chemical_list: D020413:3' Untranslated Regions; D015415:Biomarkers; D053712:Interleukin-12 Receptor beta 2 Subunit; D007376:Interleukin-2; D010835:Phytohemagglutinins; D018664:Interleukin-12; D007371:Interferon-gamma
keywords: T helper cells; atopic dermatitis; interleukin-12 receptor β2; miR-151a; microRNAs