title: Hepatotoxicity and nephrotoxicity of 3-bromopyruvate in mice.
abstract: To investigate the hepatotoxicity and nephrotoxicity of 3-Bromopyruvate (3BP) in mice.
  Fifteen nude mice were grafted subcutaneously in the left flank with MDA-MB-231 cells, then all mice were divided into control group (PBS), 3BP group (8 mg/kg), positive group (DNR: 0.8 mg/kg) when tumor volume reached approximately 100 mm3. 28 days later, tumors, livers and kidneys were stored in 4 % formalin solution and stained with hematoxylin and eosin staining. The Kunming mice experiment included control group (PBS), 3BP group (4mg/kg; 8mg/kg; 16mg/kg), positive group (DNR: 0.8 mg/kg). 24 hours later, the blood were used for the determination of hepatic damage serum biomarkers. Livers were stored in 4 % formalin solution for the later detection.
  3BP at the dose of 8mg/kg had a good effect on inhibiting tumor growth in nude mice and did not damage liver and kidney tissues. Kunming mice experiment showed 3BP at the dose of 16mg/kg did damage to liver tissues.
  3-Bromopyruvate at the dose of suppressing tumor growth did not exhibit hepatotoxicity and nephrotoxicity in nude mice, and the effect on liver was confirmed in Kunming mice.
authors.affiliation: Graduate student, Department of Pharmacy, China. Technical procedures, manuscript preparation.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Graduate student, Department of Pharmacy, China. Technical procedures.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Assistant Professor, Department of Pathology, China. Technical procedures.
authors.forename: Qili
authors.initials: Q
authors.lastname: Jin
authors.affiliation: Graduate student, Department of Pharmacy, China, Technical procedures.
authors.forename: Qixiang
authors.initials: Q
authors.lastname: Li
authors.affiliation: Assistant Professor, Department of Pharmacy, China. Conception and design of the study, critical revision.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Full Professor, Department of Pharmacy, China. Conception and design of the study.
authors.forename: Hao
authors.initials: H
authors.lastname: Liu
authors.affiliation: Assistant Professor, Department of Pharmacy, China. Conception and design of the study, critical revision.
authors.forename: Surong
authors.initials: S
authors.lastname: Zhao
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000473:pathology*; D000818:Animals; D056486:Chemical and Drug Induced Liver Injury / Q000473:pathology*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D004791:Enzyme Inhibitors / Q000633:toxicity*; D005260:Female; D007668:Kidney / Q000187:drug effects*; D008099:Liver / Q000187:drug effects*; D008114:Liver Neoplasms, Experimental / Q000188:drug therapy; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D011773:Pyruvates / Q000633:toxicity*
chemical_list: D004791:Enzyme Inhibitors; D011773:Pyruvates; C017092:bromopyruvate