title: Label-Based and Label-Free Strategies for Protein Quantitation.
abstract: The precise quantification of changes between various physiological states in a biological system is highly complex in nature. Over the past few years, in combination with classical methods, mass spectrometry based approaches have become an indispensable tool in deciphering exact abundance of proteins in composite mixtures. The technique is now well established and employs both label-based and label-free quantitation strategies. Label-based quantitation methods utilize stable isotope labels which are incorporated within the peptides, introducing an expectable mass difference within the two or more experimental conditions. In contrast, label-free proteomics quantitates both relative and absolute protein quantity by utilizing signal intensity and spectral counting of peptides. This chapter focuses on the commonly used quantitative mass spectrometry methods for high-throughput proteomic analysis.
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.
authors.forename: Sushma
authors.initials: S
authors.lastname: Anand
authors.affiliation: Department of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Bundoora, Melbourne, VIC, 3086, Australia.
authors.forename: Monisha
authors.initials: M
authors.lastname: Samuel
authors.affiliation: The Bio21 Molecular Science and Biotechnology Institute, University of Melbourne, Parkville, VIC, 3010, Australia.
authors.forename: Ching-Seng
authors.initials: CS
authors.lastname: Ang
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.
authors.forename: Shivakumar
authors.initials: S
authors.lastname: Keerthikumar
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia. S.Mathivanan@latrobe.edu.au.
authors.forename: Suresh
authors.initials: S
authors.lastname: Mathivanan
mesh_terms: D019540:Area Under Curve; D019295:Computational Biology / Q000379:methods; D007553:Isotope Labeling; D013058:Mass Spectrometry / Q000379:methods; D011506:Proteins / Q000737:chemistry / Q000378:metabolism; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D012984:Software; D057188:Workflow
chemical_list: D011506:Proteins; D020543:Proteome
keywords: Label-free quantitation; Mass spectrometry; Quantitative proteomics; Stable isotope labeling