title: Reversion of hyperhydricity in pink (Dianthus chinensis L.) plantlets by AgNO3and its associated mechanism during in vitro culture.
abstract: Hyperhydricity occurs frequently in plant tissue culture and can severely affect commercial micropropagation and genetic improvement of the cultured plantlets. Hyperhydric shoots are charaterzized by high water content, but how this occurs is still a subject of investigation. Silver ion (Ag+) can reduce the extent of hyperhydricity in plants, but its effect on the reversion of hyperhydric plantlets and the underlying mechanism of reversion has not been clarified. In this study, about 67% of the hyperhydric Dianthus chinensis L. plantlets were found to revert to normal condition when the plantlets were cultured in medium supplemented with 29.4μmolL-1AgNO3. Water content and hydrogen peroxide (H2O2) content in the guard cells of these plantlets were reduced, while stomatal aperture and water loss rate were increased. AgNO3also reduced the content of endogenous ethylene and expression of ethylene synthesis and ethylene signal transduction-associated genes. Reduced accumulation of ethylene consequently led to an increase in stomatal aperture mediated by decreased H2O2content in the guard cells. These results adequately verified the role of AgNO3in the reversion of hyperhydricity in D. chinensis L. and also provided clues for exploring the cause of excessive water accumulation in hyperhydric plants.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China. Electronic address: 21318083@mail.dlut.edu.cn.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Gao
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China. Electronic address: xx47@dlut.edu.cn.
authors.forename: Xiuying
authors.initials: X
authors.lastname: Xia
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China. Electronic address: bioeng@dlut.edu.cn.
authors.forename: Lijia
authors.initials: L
authors.lastname: An
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China. Electronic address: bzxin@mail.dlut.edu.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Xin
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China. Electronic address: lyly@mail.dlut.edu.cn.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Liang
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D029749:Dianthus / Q000187:drug effects* / Q000254:growth & development / Q000502:physiology; D005030:Ethylenes / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D006861:Hydrogen Peroxide / Q000378:metabolism; D018384:Oxidative Stress; D054046:Plant Stomata / Q000187:drug effects / Q000502:physiology; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D012835:Silver Nitrate / Q000494:pharmacology*; D014867:Water / Q000378:metabolism
chemical_list: D000975:Antioxidants; D005030:Ethylenes; D017382:Reactive Oxygen Species; D014867:Water; C036216:ethylene; D012835:Silver Nitrate; D006861:Hydrogen Peroxide
keywords: Dianthus chinensis L.; Ethylene; Hyperhydricity; Reactive oxygen species; Silver nitrate; Stomatal aperture