title: Integrins functioning in uterine endometrial stromal and epithelial cells in estrus.
abstract: Here, as a basic study in the construction of a non-cellular niche that supports artificial organization of three-dimensional endometrial tissue, we defined the types of integrin heterodimers that are expressed transcriptionally, translationally and functionally in endometrial stromal (ES) and endometrial epithelial (EE) cells isolated from the mouse uterus in estrus. Gene and protein expression of integrin subunits were analyzed at the transcriptional and translational level by real-time PCR and fluorescent immunoassay, respectively. Moreover, the functionality of integrin heterodimers was confirmed by attachment and antibody inhibition assays. Itga2, Itga5, Itga6, Itga9, Itgav, Itgb1, Itgb3 and Itgb5 in ES cells, and Itga2, Itga5, Itga6, Itga7, Itga9, Itgav, Itgb1, Itgb3, Itgb4, Itgb5 and Itga6 and in EE cells showed significantly higher transcriptional levels than the other integrin subunits. Furthermore, translational expression of the total integrin α and β subunit genes that showed increased transcription was determined in ES and EE cells. ES cells showed significantly increased adhesion to collagen I, fibronectin and vitronectin, and functional blocking of integrin α2, α5or αVsignificantly inhibited adhesion to these molecules. Moreover, EE cells showed significantly increased adhesion to collagen I, fibronectin, laminin and vitronectin, and functional blocking of integrin α2, α5, α6or αVsignificantly inhibited adhesion to these molecules. Accordingly, we confirmed that integrin α2β1, α5β1, αVβ1, αVβ3and/or αVβ5, and integrin α2β1, α5β1, α6β1and/or α6β4, αVβ1, αVβ3and/or αVβ5, actively function on the surface of ES and EE cells from mouse uterus in estrus phase, respectively.
authors.affiliation: Department of Animal Life ScienceKangwon National University, Chuncheon, Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Park
authors.affiliation: Department of Animal Life ScienceKangwon National University, Chuncheon, Korea.
authors.forename: Ji Eun
authors.initials: JE
authors.lastname: Park
authors.affiliation: Department of Animal Life ScienceKangwon National University, Chuncheon, Korea.
authors.forename: Hyun
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Animal Life ScienceKangwon National University, Chuncheon, Korea.
authors.forename: Seong Jae
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Division of Animal Resource ScienceKangwon National University, Chuncheon, Korea.
authors.forename: Jung Im
authors.initials: JI
authors.lastname: Yun
authors.affiliation: Animal Nutrition and Physiology TeamNational Institute of Animal Science, RDA, Wanju, Korea.
authors.forename: Minseok
authors.initials: M
authors.lastname: Kim
authors.affiliation: Division of Animal Resource ScienceKangwon National University, Chuncheon, Korea.
authors.forename: Kyu Hyun
authors.initials: KH
authors.lastname: Park
authors.affiliation: Department of Animal Life ScienceKangwon National University, Chuncheon, Korea stlee76@kangwon.ac.kr.|Division of Applied Animal ScienceKangwon National University, Chuncheon, Korea.
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Lee
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004717:Endometrium / Q000166:cytology / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism*; D004971:Estrus / Q000378:metabolism*; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D016023:Integrins / Q000378:metabolism*; D051379:Mice; D008813:Mice, Inbred ICR; D017154:Stromal Cells / Q000166:cytology / Q000378:metabolism*; D014599:Uterus / Q000166:cytology / Q000378:metabolism*
chemical_list: D016023:Integrins