title: Structured model and parameter estimation in plant cell cultures of Thevetia peruviana.
abstract: In this work, a mechanistic model for predicting the dynamic behavior of extracellular and intracellular nutrients, biomass production, and the main metabolites involved in the central carbon metabolism in plant cell cultures of Thevetia peruviana is presented. The proposed model is the first mechanistic model implemented for plant cell cultures of this species, and includes 28 metabolites, 33 metabolic reactions, and 61 parameters. Given the over-parametrization of the model, its nonlinear nature and the strong correlation among the effects of the parameters, a parameter estimation routine based on identifiability analysis was implemented. This routine reduces the parameter's search space by selecting the most sensitive and linearly independent parameters. Results have shown that only 19 parameters are identifiable. Finally, the model was used for analyzing the fluxes distribution in plant cell cultures of T. peruviana. This analysis shows high uptake of phosphates and parallel uptake of glucose and fructose. Furthermore, it has pointed out the main central carbon metabolism routes for promoting biomass production in this cell culture.
authors.affiliation: Research Group on Simulation, Design, Control and Optimization of Chemical Processes (SIDCOP), Faculty of Engineering, Universidad de Antioquia, Calle 67 No 53, 108, Medellín, Colombia. adriana.villegas@ucc.edu.co.|Termomec Research Group, Faculty of Engineering, Universidad Cooperativa de Colombia. Calle 48 No 28-00, Medellín, Colombia. adriana.villegas@ucc.edu.co.
authors.forename: Adriana
authors.initials: A
authors.lastname: Villegas
authors.affiliation: Research Group in Industrial Biotechnology, Faculty of Sciences, Universidad Nacional de Colombia Sede Medellín, Calle 59ª, No 63-20, Medellín, Colombia.
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Arias
authors.affiliation: Audubon Sugar Institute, LSU AgCenter. United States. 3845 Highway 75, St Gabriel, LA, 70776, USA.
authors.forename: Daira
authors.initials: D
authors.lastname: Aragón
authors.affiliation: Research Group on Simulation, Design, Control and Optimization of Chemical Processes (SIDCOP), Faculty of Engineering, Universidad de Antioquia, Calle 67 No 53, 108, Medellín, Colombia.
authors.forename: Silvia
authors.initials: S
authors.lastname: Ochoa
authors.affiliation: Research Group in Industrial Biotechnology, Faculty of Sciences, Universidad Nacional de Colombia Sede Medellín, Calle 59ª, No 63-20, Medellín, Colombia.
authors.forename: Mario
authors.initials: M
authors.lastname: Arias
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D008954:Models, Biological; D059828:Plant Cells / Q000378:metabolism*; D031052:Thevetia / Q000166:cytology* / Q000378:metabolism*
keywords: Identifiability analysis; Modelling; Parameter estimation; Plant cell cultures; Thevetia peruviana