title: Functional Characterization of Triclosan-Resistant Enoyl-acyl-carrier Protein Reductase (FabV) inPseudomonas aeruginosa.
abstract: Pseudomonas aeruginosais extremely resistant to triclosan. Previous studies have shown thatP. aeruginosaencodes a triclosan-resistant enoyl-acyl-carrier protein reductase (ENR), FabV, and that deletion offabVcausesP. aeruginosato be extremely sensitive to triclosan. In this report, we complemented aP. aeruginosa fabVdeletion strain with several triclosan-resistant ENR encoding genes, includingVibrio cholerae fabV, Bacillus subtilis fabLandEnterococcus faecalis fabK. All complemented strains restored triclosan resistance to the level of the wild-type strain, which confirmed that triclosan-resistant ENR allowsP. aeruginosato be extremely resistant to triclosan. Moreover,fabVexhibits pleiotropic effects. Deletion offabVledP. aeruginosato show attenuated swarming motility, decreased rhamnolipid, pyoverdine and acyl-homoserine lactones (AHLs) production. Complementation of thefabVmutant with any one ENR encoding gene could restore these features to some extent, in comparison with the wild-type strain. Furthermore, we found that addition of exogenous AHLs could restore thefabVmutant strain to swarm on semisolid plates and to produce more virulence factors than thefabVmutant strain. These findings indicate that deletion offabVreduced the activity of ENR inP. aeruginosa, decreased fatty acid synthesis, and subsequently depressed the production of AHLs and other virulence factors, which finally may led to a reduction in the pathogenicity ofP. aeruginosa. Therefore,fabVshould be an ideal target for the control ofP. aeruginosainfectivity.
authors.affiliation: Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University Guangzhou, China.
authors.forename: Yong-Heng
authors.initials: YH
authors.lastname: Huang
authors.affiliation: Shaanxi Engineering and Technological Research Center for Conversation and Utilization of Regional Biological Resources, College of Life Sciences, Yan'an University Yan'an, China.
authors.forename: Jin-Shui
authors.initials: JS
authors.lastname: Lin
authors.affiliation: Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University Guangzhou, China.
authors.forename: Jin-Cheng
authors.initials: JC
authors.lastname: Ma
authors.affiliation: Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, College of Life Sciences, South China Agricultural University Guangzhou, China.
authors.forename: Hai-Hong
authors.initials: HH
authors.lastname: Wang
keywords: Pseudomonas aeruginosa; acylhomoserine lactones; enoyl-acyl-carrier protein reductase; swarming; triclosan