title: Generation and Evaluation of Prophylactic mRNA Vaccines Against Allergy.
abstract: Due to the worldwide increase in allergies and a limited efficacy of therapeutic interventions, the need for prophylactic vaccination against allergies has been recognized. mRNA and DNA vaccines have demonstrated their high potential for preventing allergic sensitization by inducing an immunological bias that prevents TH2 sensitization. However, only mRNA vaccines fulfill the stringent safety requirements for vaccination of healthy children. In this chapter, we describe the generation of conventional as well as self-replicating mRNA vaccines and methods to test their prophylactic efficacy in animal models.
authors.affiliation: Division of Allergy and Immunology, Department of Molecular Biology, University of Salzburg, Hellbrunnerstraße 34, 5020, Salzburg, Austria. Richard.Weiss@sbg.ac.at.
authors.forename: Richard
authors.initials: R
authors.lastname: Weiss
authors.affiliation: Division of Allergy and Immunology, Department of Molecular Biology, University of Salzburg, Hellbrunnerstraße 34, 5020, Salzburg, Austria.
authors.forename: Sandra
authors.initials: S
authors.lastname: Scheiblhofer
authors.affiliation: Division of Allergy and Immunology, Department of Molecular Biology, University of Salzburg, Hellbrunnerstraße 34, 5020, Salzburg, Austria.
authors.forename: Josef
authors.initials: J
authors.lastname: Thalhamer
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D002460:Cell Line; D005260:Female; D006967:Hypersensitivity / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000276:immunology*; D051381:Rats; D014611:Vaccination / Q000379:methods; D014612:Vaccines / Q000276:immunology*; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000485:Allergens; D012333:RNA, Messenger; D014612:Vaccines; D019444:Vaccines, DNA
keywords: Allergy; BALB/c; Genetic immunization; Prophylactic; Self-replicating mRNA; TH1/TH2 responses; mRNA vaccine