title: Bacterial community structure within an activated sludge reactor added with phenolic compounds.
abstract: Biodegradation of phenolic compounds in bioreactors is well documented, but the changes in the bacterial populations dynamics during degradation were not that often. A glass bubble column used as reactor was inoculated with activated sludge, spiked with 2-chlorophenol, phenol and m-cresol after 28 days and maintained for an additional 56 days, while the 16S rRNA gene from metagenomic DNA was monitored. Proteobacteria (68.1%) dominated the inoculum, but the bacterial composition changed rapidly. The relative abundance of Bacteroidetes and Firmicutes decreased from 4.8 and 9.4 to <0.1 and 0.2% respectively, while that of Actinobacteria and TM7 increased from 4.8 and 2.0 to 19.2 and 16.1% respectively. Phenol application increased the relative abundance of Proteobacteria to 94.2% (mostly Brevundimonas 17.6%), while that of Bacteroidetes remained low (1.2%) until day 42. It then increased to 47.3% (mostly Leadbetterella 46.9%) at day 84. It was found that addition of phenolic compounds did not affect the relative abundance of the Alphaproteobacteria initially, but it decreased slowly while that of the Bacteroidetes increased towards the end.
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360, México, DF, Mexico. esgomez@cinvestav.mx.
authors.forename: Selene
authors.initials: S
authors.lastname: Gómez-Acata
authors.affiliation: ABACUS, Cinvestav, México, DF, Mexico.
authors.forename: Ivonne
authors.initials: I
authors.lastname: Esquivel-Ríos
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360, México, DF, Mexico.
authors.forename: Mariana Vivian
authors.initials: MV
authors.lastname: Pérez-Sandoval
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360, México, DF, Mexico.
authors.forename: Yendi
authors.initials: Y
authors.lastname: Navarro-Noya
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360, México, DF, Mexico.
authors.forename: Aketzally
authors.initials: A
authors.lastname: Rojas-Valdez
authors.affiliation: ABACUS, Cinvestav, México, DF, Mexico.
authors.forename: Frederic
authors.initials: F
authors.lastname: Thalasso
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360, México, DF, Mexico.
authors.forename: Marco
authors.initials: M
authors.lastname: Luna-Guido
authors.affiliation: Laboratory of Soil Ecology, ABACUS, Cinvestav, Avenida Instituto Politécnico Nacional 2508, 07360, México, DF, Mexico.
authors.forename: Luc
authors.initials: L
authors.lastname: Dendooven
mesh_terms: D039903:Actinobacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D001419:Bacteria / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism*; D041963:Bacteroidetes / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D001673:Biodegradation, Environmental; D019149:Bioreactors; D002733:Chlorophenols / Q000378:metabolism / Q000494:pharmacology; D003408:Cresols / Q000378:metabolism / Q000494:pharmacology; D059014:High-Throughput Nucleotide Sequencing; D056186:Metagenomics; D059013:Microbial Consortia / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D019800:Phenol / Q000378:metabolism / Q000494:pharmacology; D010636:Phenols / Q000378:metabolism* / Q000494:pharmacology*; D020560:Proteobacteria / Q000145:classification / Q000187:drug effects / Q000235:genetics / Q000502:physiology; D012336:RNA, Ribosomal, 16S; D012722:Sewage / Q000032:analysis / Q000382:microbiology*
chemical_list: D002733:Chlorophenols; D003408:Cresols; D010636:Phenols; D012336:RNA, Ribosomal, 16S; D012722:Sewage; C032538:4-cresol; D019800:Phenol; C030683:2-chlorophenol
keywords: 2-Chlorophenol; Brevundimonas; Leadbetterella; Phenol; TM7; m-Cresol