title: Thermodesulfobium acidiphilum sp. nov., a thermoacidophilic, sulfate-reducing, chemoautotrophic bacterium from a thermal site.
abstract: An obligately anaerobic, sulfate-reducing micro-organism, strain 3127-1T, was isolated from geothermally heated soil (Oil Site, Uzon Caldera, Kamchatka, Russia). The new isolate was a moderately thermoacidophilic anaerobe able to grow with H2 or formate by respiration of sulfate or thiosulfate. The pH range for growth was 3.7-6.5, with an optimum at 4.8-5.0. The temperature range for growth was 37-65 °C, with an optimum at 55 °C. The G+C content of the genomic DNA was 33.7 mol%. The genome of strain 3127-1T contained two almost identical 16S rRNA genes, differing by a single nucleotide substitution. The closest 16S rRNA gene sequence of a validly published species belonged to Thermodesulfobium narugense Na82T (99.5 % similarity). However, the average nucleotide identity of the genomes of strain 3127-1T and T. narugense Na82T and the predicted DNA-DNA hybridization value (GGDC 2.1 blast+, formula 2) were as low as 86 and 32.5±2.5 %, respectively. This, together with phenotypic data, showed the new isolate to belong to a novel species, for which the name Thermodesulfobium acidiphilum sp. nov. is proposed. The type strain is 3127-1T (=DSM 102892T=VKM B-3043T).
authors.affiliation: Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 33-2 Leninsky prospect, 119071, Moscow, Russia.
authors.forename: Evgenii N
authors.initials: EN
authors.lastname: Frolov
authors.affiliation: Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 33-2 Leninsky prospect, 119071, Moscow, Russia.
authors.forename: Ilya V
authors.initials: IV
authors.lastname: Kublanov
authors.affiliation: Immanuel Kant Baltic Federal University, 3 Botkina str., 236016, Kaliningrad, Russia.
authors.forename: Stepan V
authors.initials: SV
authors.lastname: Toshchakov
authors.affiliation: Immanuel Kant Baltic Federal University, 3 Botkina str., 236016, Kaliningrad, Russia.
authors.forename: Nazar I
authors.initials: NI
authors.lastname: Samarov
authors.affiliation: Gubkin University, 65-1 Leninskiy prospect, 119991, Moscow, Russia.
authors.forename: Andrei A
authors.initials: AA
authors.lastname: Novikov
authors.affiliation: Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 33-2 Leninsky prospect, 119071, Moscow, Russia.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Lebedinsky
authors.affiliation: Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 33-2 Leninsky prospect, 119071, Moscow, Russia.
authors.forename: Elizaveta A
authors.initials: EA
authors.lastname: Bonch-Osmolovskaya
authors.affiliation: Winogradsky Institute of Microbiology, Research Centre of Biotechnology RAS, 33-2 Leninsky prospect, 119071, Moscow, Russia.
authors.forename: Nikolai A
authors.initials: NA
authors.lastname: Chernyh
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D000068536:Bacillota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D045482:Hot Springs / Q000382:microbiology*; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012426:Russia; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S