title: The effect of serum on the proliferation of bone marrow-derived mesenchymal stem cells from aged donors and donors with or without chronic heart failure.
abstract: Many clinical studies of regenerative medicine using bone marrow-derived mesenchymal stem cells (MSCs) have been conducted globally. We initiated clinical studies using MSCs in 2001 and have now treated over 100 cases with patients aged 0-92 years. In a few cases involving patients with chronic heart failure (CHF), we observed that MSCs proliferated poorly. This contrasts with cell therapy studies wherein MSCs of patients with CHF were used for treatment. The effects of serum on the proliferation of MSCs from donors with normal heart function and with CHF have not been reported. Moreover, whether cell therapy is effective for elderly patients remains uncertain. Therefore, characterization of MSCs from aged donors and/or donors with CHF is urgently required. We retrospectively analysed the population doubling times (PDTs) of MSCs between the first and second passages. Although we had data for many samples of well-expanded MSCs from aged donors, a positive correlation was observed between donor age and PDT. A trend towards reduced variance in PDTs was observed in MSCs supplemented with fetal bovine serum (FBS) compared with those supplemented with autologous serum. When autologous serum was used, the average PDT of MSCs from donors with CHF was significantly longer than that of MSCs from donors without CHF. In contrast, when FBS was used, similar PDTs were observed in MSCs from donors with and without CHF. Thus, FBS promotes MSC expansion even from donors with CHF and MSC-based regenerative medicine might be feasible even for elderly patients. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Medical and Biological Engineering Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Osaka, Japan.
authors.forename: Hideki
authors.initials: H
authors.lastname: Kubo
authors.affiliation: Medical and Biological Engineering Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Osaka, Japan.
authors.forename: Kanae
authors.initials: K
authors.lastname: Takamura
authors.affiliation: Nagaya Clinic for Internal Medicine, Osaka, Japan.
authors.forename: Noritoshi
authors.initials: N
authors.lastname: Nagaya
authors.affiliation: Medical and Biological Engineering Research Group, Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology, Osaka, Japan.
authors.forename: Hajime
authors.initials: H
authors.lastname: Ohgushi
mesh_terms: D000368:Aged; D000954:Antigens, Surface / Q000378:metabolism; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D049109:Cell Proliferation; D006333:Heart Failure / Q000473:pathology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D044967:Serum / Q000378:metabolism*; D014019:Tissue Donors
chemical_list: D000954:Antigens, Surface
keywords: autologous serum; bone marrow; fetal bovine serum; heart failure; mesenchymal stem cells; population doubling time