title: Roseomonas nepalensis sp. nov., isolated from oil-contaminated soil.
abstract: A wine-red-coloured, Gram-stain-negative, aerobic, non-motile and coccus-shaped bacterium, designated strain G-3-5T, was isolated from oil-contaminated soil of Biratnagar, Morang, Nepal, during a study of oil-utilizing bacteria. This strain was catalase-negative and oxidase-positive. It was able to grow at 10-37 °C, at pH 6.0-10.0 and with 0.02-1.02 % (w/v) NaCl. This strain was taxonomically characterized by a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain G-3-5T belongs to the genus Roseomonas and is closely related to Roseomonas vinacea CPCC 100056T (97.81 % sequence similarity), Roseomonas aerilata 5420S-30T (96.68 %), Roseomonas pecuniae N75T (96.15 %), 'Roseomonas aceris' R-1 (95.75 %) and Roseomonas rosea 173/96T (95.30 %). The only respiratory quinone was ubiquinone-10. The polar lipid profile revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The major fatty acids of strain G-3-5T were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, C18 : 0 and C18 : 1 2-OH. The genomic DNA G+C content of this novel strain was 68.3 mol%. The DNA-DNA relatedness between strain G-3-5T and Roseomonas. vinacea KACC 13934T was 26.3 %. The morphological, physiological, chemotaxonomic and phylogenetic analyses clearly distinguished this strain from its closest phylogenetic neighbours. Thus, strain G-3-5T represents a novel species of the genus Roseomonas, for which the name Roseomonas nepalensis sp. nov. is proposed. The type strain is G-3-5T (=KEMB 9005-416T=KACC 18908T=JCM 31470T).
authors.affiliation: Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
authors.forename: Dhiraj Kumar
authors.initials: DK
authors.lastname: Chaudhary
authors.affiliation: Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Gyeonggi-Do 16227, Republic of Korea.
authors.forename: Jaisoo
authors.initials: J
authors.lastname: Kim
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D042082:Methylobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009390:Nepal; D009693:Nucleic Acid Hybridization; D059392:Petroleum Pollution; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2