title: Honeybee product therapeutic as stem cells homing for ovary failure.
abstract: OBJECTIVE
  Complexity of the method of isolation, cultivationin vitroand the expensive cost of transplantation process of stem cells, it would require an innovation to homing and differentiation of stem cells and increase folliculogenesis. The stem cells homing was achieved through the provision of food or beverages derived from natural materials like honeybee product. Through honeybee product, there will be homing of stem cells and accompany with the sources from the body itself will take place in regeneration of the ovary.
  
  
  METHODS
  Female rats model of degenerative ovary was obtained through food fasting but still have drinking water for 5 days. It caused malnutrition and damage of the ovarian tissue. The administration of 50% honeybee product (T1) was performed for 10 consecutive days, while the positive control group (T0+) was fasted and not given honeybee product and the negative control (T0-) not fasted and without honeybee product. Observations were taken for homing of stem cells, raised of folliculogenesis, differentiation of stem cells, and regeneration of the ovarian tissue using routine H&E staining.
  
  
  RESULTS
  Homing of stem cells shown the vascular endothelial growth factor and granulocyte colony-stimulating factor expression; enhancement of folliculogenesis was indicated by an increase of follicle dee Graaf count; enhancement of differentiation of stem cells was indicated by growth differentiation factor-9 expression; and regeneration of ovarian tissue indicated by intact ovarian tissue with growing follicles.
  
  
  CONCLUSIONS
  Honeybee product can be induced endogenous stem cells in regeneration of ovary failure due to malnutrition.
authors.affiliation: Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia; Stem Cells Research Division of Institute Tropical Disease, Universitas Airlangga, Surabaya, East Java, Indonesia.
authors.forename: Erma
authors.initials: E
authors.lastname: Safitri
authors.affiliation: Department of Veterinary Pathology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
authors.forename: Thomas V
authors.initials: TV
authors.lastname: Widiyatno
authors.affiliation: Department of Parasitology, Faculty of Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia.
authors.forename: R Heru
authors.initials: RH
authors.lastname: Prasetyo
keywords: honeybee product; ovary failure; stem cells homing