title: Astaxanthin conjugated polypyrrole nanoparticles as a multimodal agent for photo-based therapy and imaging.
abstract: Polymeric nanoparticles are emerging as promising candidates for photo-based therapy and imaging due to their versatile chemical properties and easy fabrication and functionalization. In the present study we synthesized polypyrrole nanoparticles by stabilization with astaxanthin conjugated bovine serum albumin polymer (PPy@BSA-Astx). The synthesized nanoparticles were biocompatible with MBA-MD-231 and HEK-293 cells. Interestingly, the fabricated nanoparticles produced reactive oxygen species under 808-nm laser exposure and exerted a hyperthermic effect when the power density of the laser was increased. The photodynamic efficiency of PPy@BSA-Astx was measured by DPBF assay, and it was found to generate sufficient amount of reactive radicals to kill the cells at a power density of 0.3W/cm2. In photothermal aspect, the temperature level was reached to 57°C within 5min at 1W/cm2power density, at the concentration of 50μg/mL. The in vitro cell toxicity studies showed concentration dependent photothermal and photodynamic toxicity. Fluorescence microscopic investigation explored the cell death and intra-cellular organ destruction by photodynamic treatment. In addition, we observed a strong photoacoustic signal from a tissue mimicking phantom study of nanoparticle treated MBA-MD-231 cells. In conclusion, the fabricated PPy@BSA-Astx nanoparticles can be used as photoacoustic imaging based prognostic agents for photothermal or photodynamic treatment.
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Subramaniyan
authors.initials: S
authors.lastname: Bharathiraja
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Panchanathan
authors.initials: P
authors.lastname: Manivasagan
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Yun-Ok
authors.initials: YO
authors.lastname: Oh
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Madhappan Santha
authors.initials: MS
authors.lastname: Moorthy
authors.affiliation: Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Hansu
authors.initials: H
authors.lastname: Seo
authors.affiliation: Department of Biomedical Engineering and Center for Marine-Integrated Biotechnology (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea.
authors.forename: Nhat Quang
authors.initials: NQ
authors.lastname: Bui
authors.affiliation: Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Republic of Korea; Department of Biomedical Engineering, Pukyong National University, Busan 48513, Republic of Korea; Department of Biomedical Engineering and Center for Marine-Integrated Biotechnology (BK21 Plus), Pukyong National University, Busan 48513, Republic of Korea. Electronic address: jungoh@pknu.ac.kr.
authors.forename: Junghwan
authors.initials: J
authors.lastname: Oh
mesh_terms: D000818:Animals; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured / Q000473:pathology; D003952:Diagnostic Imaging / Q000379:methods*; D004305:Dose-Response Relationship, Drug; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D061088:Photoacoustic Techniques / Q000379:methods*; D010789:Phototherapy / Q000379:methods*; D011108:Polymers / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D024341:Xanthophylls / Q000737:chemistry / Q000494:pharmacology
chemical_list: D011108:Polymers; D011758:Pyrroles; D024341:Xanthophylls; D012710:Serum Albumin, Bovine; C067635:polypyrrole; C005948:astaxanthine
keywords: Albumin-polypyrrole; Astaxanthin; Photoacoustic; Photodynamic; Photothermal