title: Quantitative Analysis of Exosomal miRNA via qPCR and Digital PCR.
abstract: Extracellular vesicles, such as exosomes and microvesicles, have been shown to contain potential microRNA (miRNA) biomarkers that may be utilized in the diagnosis of various diseases from cancer to neurological disorders. The unique nature of the extracellular vesicle bilayer allows miRNA to be protected from degradation making it an ideal source of material for biomarkers discovery from both fresh and archived samples. Here we describe the quantitative analysis of miRNA isolated from exosomes by quantitative PCR and digital PCR.
authors.affiliation: Department of Biochemistry and Molecular Biology, The University of Melbourne, Melbourne, VIC, Australia.|Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne, Melbourne, VIC, Australia.
authors.forename: Shayne A
authors.initials: SA
authors.lastname: Bellingham
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Room 415, LIMS1, Bundoora, Melbourne, VIC, 3086, Australia.
authors.forename: Mitch
authors.initials: M
authors.lastname: Shambrook
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia. Andrew.hill@latrobe.edu.au.
authors.forename: Andrew F
authors.initials: AF
authors.lastname: Hill
mesh_terms: D015415:Biomarkers; D019295:Computational Biology / Q000379:methods; D055354:Exosomes / Q000378:metabolism*; D015870:Gene Expression; D035683:MicroRNAs / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D034443:RNA Transport; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D015415:Biomarkers; D035683:MicroRNAs
keywords: Biomarker; Digital PCR; Exosomes; Quantitative PCR; RNA isolation; Taqman assay; miRNA