title: Harmonized Collaborative Validation of Aflatoxins and Sterigmatocystin in White Rice and Sorghum by Liquid Chromatography Coupled to Tandem Mass Spectrometry.
abstract: An interlaboratory study was performed in eight laboratories to validate a liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for the simultaneous determination of aflatoxins and sterigmatocystin (STC) in white rice and sorghum (Sorghum bicolor). Fortified samples (at three different levels) of white rice and sorghum were extracted, purified through a solid-phase extraction (SPE) column, and then analyzed by LC/MS/MS. The apparent recoveries (ARs) ranged from 78.8% to 95.0% for aflatoxins and from 85.3% to 96.7% for STC. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDR) of aflatoxins were in the ranges 7.9%-33.8% and 24.4%-81.0%, respectively. For STC, the RSDrranged from 7.1% to 40.2% and the RSDR ranged from 28.1% to 99.2%. The Horwitz ratio values for the aflatoxins and STC ranged from 0.4 to 1.2 in white rice and from 0.3 to 1.0 in sorghum, respectively. These results validated this method for the simultaneous determination of aflatoxins and STC by LC/MS/MS after SPE column cleanup. The percentages of satisfactoryZ-score values (|Z| ≤ 2) were the following: for white rice, 100% for aflatoxins and STC; for sorghum, 100%, except in data from two laboratories for STC (0.3 μg/kg). This validated that the LC/MS/MS method was successfully applied for the determination of aflatoxins and STC in 20 white rice and 20 sorghum samples sourced from Korean markets.
authors.affiliation: Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea. ohe0904@hanmail.net.
authors.forename: Hyun Ee
authors.initials: HE
authors.lastname: Ok
authors.affiliation: Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea. TianFei@cau.ac.kr.
authors.forename: Fei
authors.initials: F
authors.lastname: Tian
authors.affiliation: Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea. eyhongs@gmail.com.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Hong
authors.affiliation: Food Contaminants Division, National Institute of Food & Drug Safety Evaluation, Osong 28159, Korea. ojpaek92@korea.kr.
authors.forename: Ockjin
authors.initials: O
authors.lastname: Paek
authors.affiliation: Food Contaminants Division, National Institute of Food & Drug Safety Evaluation, Osong 28159, Korea. cinee@korea.kr.
authors.forename: Sheen-Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Food Contaminants Division, National Institute of Food & Drug Safety Evaluation, Osong 28159, Korea. dongsul@korea.kr.
authors.forename: Dongsul
authors.initials: D
authors.lastname: Kim
authors.affiliation: Advanced Food Safety Research Group, BK21 Plus, School of Food Science and Technology, Chung-Ang University, Anseong 17546, Korea. hschun@cau.ac.kr.
authors.forename: Hyang Sook
authors.initials: HS
authors.lastname: Chun
mesh_terms: D000348:Aflatoxins / Q000032:analysis*; D002853:Chromatography, Liquid; D005506:Food Contamination / Q000032:analysis; D012275:Oryza / Q000737:chemistry*; D015203:Reproducibility of Results; D045868:Sorghum / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D000348:Aflatoxins
keywords: aflatoxins; liquid chromatography–tandem mass spectrometry; simultaneous determination; sterigmatocystin