title: An E3 Ubiquitin Ligase-BAG Protein Module Controls Plant Innate Immunity and Broad-Spectrum Disease Resistance.
abstract: Programmed cell death (PCD) and immunity in plants are tightly controlled to promote antimicrobial defense while preventing autoimmunity. However, the mechanisms contributing to this immune homeostasis are poorly understood. Here, we isolated a rice mutant ebr1 (enhanced blight and blast resistance 1) that shows enhanced broad-spectrum bacterial and fungal disease resistance, but displays spontaneous PCD, autoimmunity, and stunted growth. EBR1 encodes an E3 ubiquitin ligase that interacts with OsBAG4, which belongs to the BAG (Bcl-2-associated athanogene) family that functions in cell death, growth arrest, and immune responses in mammals. EBR1 directly targets OsBAG4 for ubiquitination-mediated degradation. Elevated levels of OsBAG4 in rice are necessary and sufficient to trigger PCD and enhanced disease resistance to pathogenic infection, most likely by activating pathogen-associated molecular patterns-triggered immunity (PTI). Together, our study suggests that an E3-BAG module orchestrates innate immune homeostasis and coordinates the trade-off between defense and growth in plants.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Quanyuan
authors.initials: Q
authors.lastname: You
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Keran
authors.initials: K
authors.lastname: Zhai
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China; State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China.
authors.forename: Donglei
authors.initials: D
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Weibing
authors.initials: W
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jingni
authors.initials: J
authors.lastname: Wu
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China; University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Junzhong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Pan
authors.affiliation: Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Wang
authors.affiliation: China National Rice Research Institute, Hangzhou 310006, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Zhu
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
authors.forename: Yikun
authors.initials: Y
authors.lastname: Jian
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Jiyun
authors.initials: J
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Yiwen
authors.initials: Y
authors.lastname: Deng
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China.
authors.forename: Qun
authors.initials: Q
authors.lastname: Li
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China.
authors.forename: Yonggen
authors.initials: Y
authors.lastname: Lou
authors.affiliation: Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Xie
authors.affiliation: National Key Laboratory of Plant Molecular Genetics and National Center of Plant Gene Research, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China; Collaborative Innovation Center of Genetics and Development, Institute of Plant Physiology & Ecology, Chinese Academy of Sciences, Shanghai 200032, China. Electronic address: zhhe@sibs.ac.cn.
authors.forename: Zuhua
authors.initials: Z
authors.lastname: He
mesh_terms: D015551:Autoimmunity; D001419:Bacteria / Q000472:pathogenicity; D016923:Cell Death; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000276:immunology*; D005658:Fungi / Q000472:pathogenicity; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant / Q000235:genetics; D006801:Humans; D007113:Immunity, Innate; D009154:Mutation; D012275:Oryza / Q000235:genetics / Q000276:immunology / Q000382:microbiology; D010641:Phenotype; D010935:Plant Diseases / Q000276:immunology* / Q000382:microbiology; D057865:Plant Immunity / Q000276:immunology*; D018515:Plant Leaves / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D034622:RNA Interference; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000276:immunology* / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D010940:Plant Proteins; D044767:Ubiquitin-Protein Ligases
keywords: BAG protein; E3 ubiquitin ligase; autoimmunity; broad-spectrum disease resistance; defense cost; molecular module; programmed cell death; rice