title: The EB66® cell line as a valuable cell substrate for MVA-based vaccines production.
abstract: The selection of a cell substrate is a critical step for the development and manufacturing of a viral vaccine candidate. Several parameters such as cell susceptibility and permissiveness to the viral pathogens but also performance in terms of viral antigens quality and production yields are important considerations when identifying the ideal match between a viral vaccine and cell substrate. The modified vaccinia virus Ankara (MVA) is a replication-deficient viral vector that holds great promise as a vaccine platform, however only limited cell substrates have been tested or are available for industrialization. Here we evaluate the duck embryo-derived EB66® cell line as potential cell substrate for MVA production. To this end, we used two recombinant MVA constructs and demonstrated that EB66® cells are propagating the tested MVA viruses very efficiently, while preserving viral attenuation and transgene expression for up to 20 serial passages. Furthermore we developed upstream and downstream processes that enable industrialization of the virus production. In conclusion, we showed that EB66® cells can be used as potent cell substrate for MVA-based vaccines and represent therefore an attractive alternative for vaccine production.
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France. Electronic address: arnaud.leon@valneva.com.
authors.forename: Arnaud
authors.initials: A
authors.lastname: Léon
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France.
authors.forename: Anne-Laure
authors.initials: AL
authors.lastname: David
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Madeline
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Guianvarc'h
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France.
authors.forename: Elodie
authors.initials: E
authors.lastname: Dureau
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Champion-Arnaud
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hebben
authors.affiliation: Transgene SA, 400 boulevard Gonthier d'Andernach - Parc d'Innovation - CS80166, 67405 Illkirch Graffenstaden cedex, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Huss
authors.affiliation: Virbac Animal, Health, 13ème rue LID, 06515 Carros, France.
authors.forename: Benoît
authors.initials: B
authors.lastname: Chatrenet
authors.affiliation: Valneva SE, 6 rue Alain Bombard, 44800 Saint-Herblain, France.
authors.forename: Klaus
authors.initials: K
authors.lastname: Schwamborn
mesh_terms: D000818:Animals; D000956:Antigens, Viral; D002460:Cell Line; D004372:Ducks; D004625:Embryo, Nonmammalian / Q000166:cytology; D006801:Humans; D012692:Serial Passage; D019076:Transgenes; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014616:Vaccinia virus / Q000235:genetics* / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D014765:Viral Vaccines; D014776:Virus Cultivation; D014779:Virus Replication
chemical_list: D000956:Antigens, Viral; C560489:MVA vaccine; D014613:Vaccines, Attenuated; D019444:Vaccines, DNA; D014765:Viral Vaccines
keywords: EB66® cells; Manufacturing process; Modified vaccinia virus Ankara; Vaccine production