title: Low-Intensity Ultrasound Combined with Hematoporphyrin Monomethyl Ether in the Treatment of Experimental Periodontitis in Rats.
abstract: Objectives. This study aims to evaluate the efficacy of hematoporphyrin monomethyl ether- (HMME-) mediated sonodynamic therapy (SDT) on experimental periodontal disease in rats.Methods. Periodontal disease was induced by submerging ligatures at the first maxillary molar subgingival region in forty-eight male SD rats. After 30 days, the ligatures were removed. The rats were randomly allocated into four groups; the experimental SDT group was treated through hypodermic injection of 40μg/mL HMME and 3 W/cm2low-intensity ultrasound irradiation (1 MHz, 600 s). Those in control groups received 40μg/mL HMME alone (control 1 group) or 3 W/cm2ultrasound irradiation alone (control 2 group) or were subjected to neither HMME nor ultrasound (control 3 group). After 10 days of treatment, all rats were euthanized, the maxilla was obtained for histological examination, and the alveolar bone level was evaluated by histometric analysis.Results. The control groups showed more bone loss (P< 0.05) after 10 days of treatment than the SDT group. There is no significant difference among the control groups (P> 0.05).Conclusions. HMME mediated SDT was an effective therapy of experimental periodontal tissue in rats.
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors.forename: Deshu
authors.initials: D
authors.lastname: Zhuang
authors.affiliation: Department of Cadre's Ward, The First Hospital of Harbin City, Harbin 15001, China.
authors.forename: Zongshan
authors.initials: Z
authors.lastname: Ji
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors.forename: Liangjia
authors.identifier: 0000-0002-7821-8267
authors.initials: L
authors.lastname: Bi
authors.affiliation: Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin 150001, China.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Wang
authors.affiliation: Condensed Matter Science and Technology Institute, Harbin Institute of Technology, Harbin 150080, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Condensed Matter Science and Technology Institute, Harbin Institute of Technology, Harbin 150080, China; Department of Mathematics and Materials Research Institute, The Pennsylvania State University, University Park, PA 16802, USA.
authors.forename: Wenwu
authors.initials: W
authors.lastname: Cao
mesh_terms: D000539:Alveolar Process / Q000187:drug effects / Q000473:pathology; D000818:Animals; D003131:Combined Modality Therapy; D005881:Gingiva / Q000187:drug effects / Q000473:pathology; D006415:Hematoporphyrins / Q000494:pharmacology / Q000627:therapeutic use*; D008297:Male; D010518:Periodontitis / Q000188:drug therapy / Q000473:pathology / Q000628:therapy*; D017207:Rats, Sprague-Dawley; D019237:Tooth Cervix / Q000187:drug effects / Q000473:pathology; D014465:Ultrasonics
chemical_list: D006415:Hematoporphyrins; C494379:hematoporphyrin monomethyl ether