title: Sulforaphane induces differential modulation of mitochondrial biogenesis and dynamics in normal cells and tumor cells.
abstract: Antioxidant-based chemotherapy has been intensely debated. Herein, we show that sulforaphane (SFN) induced mitochondrial biogenesis followed by mitochondrial fusion in a kidney cell line commonly used in nephroprotective models. At the same concentration and exposure time, SFN induced cell death in prostate cancer cells accompanied by mitochondrial biogenesis and fragmentation. Stabilization of the nuclear factor E2-related factor-2 (Nrf2) could be associated with these effects in the tumor cell line. An increase in the peroxisome proliferator-activated receptor-γ co-activator-1α (PGC1α) level and a decrease in the hypoxia-inducible factor-1α (HIF1α) level would suggest a possible metabolic shift. The knockdown in the nuclear respiratory factor-1 (NRF1) attenuated the SFN-induced effect on prostate cancer cells demonstrating that mitochondrial biogenesis plays an important role in cell death for this kind of tumor cells. This evidence supports SFN as a potential antineoplastic agent that could inhibit tumor development and could protect normal tissues by modulating common processes.
authors.affiliation: Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
authors.forename: Mario
authors.initials: M
authors.lastname: Negrette-Guzmán
authors.affiliation: Unidad de Investigación en Enfermedades Oncológicas, Hospital Infantil de México Federico Gómez, Mexico City 06720, Mexico.
authors.forename: Sara
authors.initials: S
authors.lastname: Huerta-Yepez
authors.affiliation: Unidad de Investigación Médica en Enfermedades Oncológicas, Hospital de Oncología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social (IMSS), Mexico City 06720, Mexico.
authors.forename: Mario I
authors.initials: MI
authors.lastname: Vega
authors.affiliation: Sección de Patología Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14000, Mexico.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: León-Contreras
authors.affiliation: Sección de Patología Experimental, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14000, Mexico.
authors.forename: Rogelio
authors.initials: R
authors.lastname: Hernández-Pando
authors.affiliation: Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico.
authors.forename: Omar Noel
authors.initials: ON
authors.lastname: Medina-Campos
authors.affiliation: Unidad de Investigación en Enfermedades Oncológicas, Hospital Infantil de México Federico Gómez, Mexico City 06720, Mexico.
authors.forename: Esteban
authors.initials: E
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Fisiopatología Renal, Departamento de Nefrología, Instituto Nacional de Cardiología Ignacio Chavez, Mexico City 14080, Mexico.
authors.forename: Edilia
authors.initials: E
authors.lastname: Tapia
authors.affiliation: Departamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México, Mexico City 04510, Mexico. Electronic address: pedraza@unam.mx.
authors.forename: José
authors.initials: J
authors.lastname: Pedraza-Chaverri
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000378:metabolism; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002478:Cells, Cultured; D006801:Humans; D017879:Isothiocyanates / Q000494:pharmacology*; D008297:Male; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism; D051267:NF-E2-Related Factor 2 / Q000378:metabolism; D001678:Organelle Biogenesis; D047492:Peroxisome Proliferator-Activated Receptors / Q000378:metabolism; D011467:Prostate / Q000187:drug effects*; D011471:Prostatic Neoplasms / Q000188:drug therapy*; D015398:Signal Transduction / Q000187:drug effects; D013454:Sulfoxides
chemical_list: D000970:Antineoplastic Agents; D000975:Antioxidants; D017879:Isothiocyanates; D051267:NF-E2-Related Factor 2; D047492:Peroxisome Proliferator-Activated Receptors; D013454:Sulfoxides; C016766:sulforaphane
keywords: Cancer; Cell death; Mitochondrial biogenesis; Mitochondrial dynamics; Nuclear factor E2-related factor 2; Sulforaphane