title: PMS1T, producing phased small-interfering RNAs, regulates photoperiod-sensitive male sterility in rice.
abstract: Phased small-interfering RNAs (phasiRNAs) are a special class of small RNAs, which are generated in 21- or 24-nt intervals from transcripts of precursor RNAs. Although phasiRNAs have been found in a range of organisms, their biological functions in plants have yet to be uncovered. Here we show that phasiRNAs generated by the photopheriod-sensetive genic male sterility 1 (Pms1) locus were associated with photoperiod-sensitive male sterility (PSMS) in rice, a germplasm that started the two-line hybrid rice breeding. The Pms1 locus encodes a long-noncoding RNA PMS1T that was preferentially expressed in young panicles. PMS1T was targeted by miR2118 to produce 21-nt phasiRNAs that preferentially accumulated in the PSMS line under long-day conditions. A single nucleotide polymorphism in PMS1T nearby the miR2118 recognition site was critical for fertility change, likely leading to differential accumulation of the phasiRNAs. This result suggested possible roles of phasiRNAs in reproductive development of rice, demonstrating the potential importance of this RNA class as regulators in biological processes.
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Yourong
authors.initials: Y
authors.lastname: Fan
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jiangyi
authors.initials: J
authors.lastname: Yang
authors.affiliation: Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711.
authors.forename: Sandra M
authors.initials: SM
authors.lastname: Mathioni
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jinsheng
authors.initials: J
authors.lastname: Yu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Shen
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xuefei
authors.initials: X
authors.lastname: Yang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Cai
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Caiguo
authors.initials: C
authors.lastname: Xu
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Xianghua
authors.initials: X
authors.lastname: Li
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Plant and Soil Sciences, Delaware Biotechnology Institute, University of Delaware, Newark, DE 19711.
authors.forename: Blake C
authors.identifier: 0000-0003-3436-6097
authors.initials: BC
authors.lastname: Meyers
authors.affiliation: National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China; qifazh@mail.hzau.edu.cn.
authors.forename: Qifa
authors.initials: Q
authors.lastname: Zhang
mesh_terms: D002874:Chromosome Mapping; D003001:Cloning, Molecular; D020869:Gene Expression Profiling; D018506:Gene Expression Regulation, Plant; D017343:Genes, Plant; D056426:Genetic Loci; D005819:Genetic Markers; D016366:Open Reading Frames; D012275:Oryza / Q000235:genetics*; D010641:Phenotype; D017440:Photoperiod; D051479:Plant Infertility / Q000235:genetics*; D010940:Plant Proteins / Q000235:genetics* / Q000502:physiology; D030821:Plants, Genetically Modified; D020641:Polymorphism, Single Nucleotide; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics*; D018749:RNA, Plant / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins; D062085:RNA, Long Noncoding; D018749:RNA, Plant; D034741:RNA, Small Interfering
keywords: long-noncoding RNA; phasiRNA; photoperiod-sensitive male sterility