title: TM4SF1 promotes the self-renewal of esophageal cancer stem-like cells and is regulated by miR-141.
abstract: Cancer stem-like cells have been identified in primary human tumors and cancer cell lines. Previously we found TM4SF1 gene was highly expressed in side population (SP) cells from esophageal squamous cell carcinoma (ESCC) cell lines, but the role and underlying mechanism of TM4SF1 in ESCC remain unclear. In this study, we observed TM4SF1 was up-regulated but miR-141 was down-regulated in SP cells isolated from ESCC cell lines. TM4SF1 could stimulate the self-renewal ability and carcinogenicity of esophageal cancer stem-like cells, and promote cell invasion and migration. In miR-141 overexpression cells, the expression of TM4SF1 was significantly reduced. We also found that overexpression of miR-141 could abolish the self-renewal ability and carcinogenicity of esophageal cancer stem-like cells and decrease cell invasion and migration by suppressing TM4SF1. Consequently, TM4SF1 is a direct target gene of miR-141. The regulation of TM4SF1 by miR-141 may play an important role in controlling self-renewals of esophageal cancer stem-like cells. It may also promote the development of new therapeutic strategies and efficient drugs to target ESCC stem-like cells.
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xue
authors.affiliation: Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.|Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China.
authors.forename: Xiying
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.|Current address: Department of Pathology, Beijing ChaoYang Hospital, Capital Medical University, Beijing, China.
authors.forename: Xingran
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.|Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Deng
authors.affiliation: Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.|Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Mao
authors.affiliation: Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.|Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Guo
authors.affiliation: Department of Cancer Epidemiology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.
authors.forename: Jinhu
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Qinqxia
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Oncology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Liuxing
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Etiology and Carcinogenesis and State Key Laboratory of Molecular Oncology, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences (CAMS) & Peking Union Medical College (PUMC), Beijing, China.|Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Beijing, China.
authors.forename: Shih-Hsin
authors.initials: SH
authors.lastname: Lu
mesh_terms: D000818:Animals; D000954:Antigens, Surface / Q000235:genetics / Q000378:metabolism*; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis; D014408:Biomarkers, Tumor; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D002465:Cell Movement; D049109:Cell Proliferation; D000066673:Cell Self Renewal / Q000235:genetics*; D019008:Drug Resistance, Neoplasm; D004938:Esophageal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics*; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D009367:Neoplasm Staging; D014411:Neoplastic Stem Cells / Q000378:metabolism / Q000473:pathology*; D011379:Prognosis; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D000954:Antigens, Surface; D000970:Antineoplastic Agents; D014408:Biomarkers, Tumor; C533895:MIRN141 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; C074075:TM4SF1 protein, human
keywords: ESCC; TM4SF1; cancer stem-like cells; esophageal cancer; miR-141