title: Hydrodynamic HBV Transfection Mouse Model.
abstract: Hydrodynamic HBV transfection mouse model is an established method of the last decade where macromolecules, non-normally permeable to cell membrane, are delivered intracellular. The basic principle is that a large volume of solution, containing HBV plasmid construct, is infused rapidly in circulation to permit the preferential entrance of these macromolecules to liver parenchymal cells. The aim of this chapter is to describe the basic principles of the hydrodynamic HBV transfection in mouse models.
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Li-Ling
authors.initials: LL
authors.lastname: Wu
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan.
authors.forename: Hurng-Yi
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei, 100, Taiwan. peijerchen@ntu.edu.tw.
authors.forename: Pei-Jer
authors.initials: PJ
authors.lastname: Chen
mesh_terms: D032761:Animal Experimentation; D000818:Animals; D004279:DNA, Viral; D000229:Dependovirus / Q000235:genetics; D005822:Genetic Vectors / Q000008:administration & dosage / Q000235:genetics*; D006515:Hepatitis B virus / Q000235:genetics*; D051379:Mice; D010957:Plasmids / Q000235:genetics; D014162:Transfection / Q000379:methods*
chemical_list: D004279:DNA, Viral
keywords: Gene transfer; HBV persistence; Hydrodynamic injection; In vivo transfection; Liver tolerance