title: Phenol-degrading anode biofilm with high coulombic efficiency in graphite electrodes microbial fuel cell.
abstract: A microbial fuel cell (MFC), with graphite electrodes as both the anode and cathode, was operated with a soil-free anaerobic consortium for phenol degradation. This phenol-degrading MFC showed high efficiency with a current density of 120 mA/m2and a coulombic efficiency of 22.7%, despite the lack of a platinum catalyst cathode and inoculation of sediment/soil. Removal of planktonic bacteria by renewing the anaerobic medium did not decrease the performance, suggesting that the phenol-degrading MFC was not maintained by the planktonic bacteria but by the microorganisms in the anode biofilm. Cyclic voltammetry analysis of the anode biofilm showed distinct oxidation and reduction peaks. Analysis of the microbial community structure of the anode biofilm and the planktonic bacteria based on 16S rRNA gene sequences suggested that Geobacter sp. was the phenol degrader in the anode biofilm and was responsible for current generation.
authors.affiliation: Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan; EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Institute of Materials and Systems for Sustainability (IMaSS), Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Zhang
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Li
authors.affiliation: EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Chunfang
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Environmental Engineering and Architecture, Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Institute of Materials and Systems for Sustainability (IMaSS), Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Zhixing
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan; EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Institute of Materials and Systems for Sustainability (IMaSS), Nagoya University, Chikusa, Nagoya 464-8603, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Awata
authors.affiliation: Department of Civil Engineering, Graduate School of Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan; EcoTopia Science Institute, Nagoya University, Chikusa, Nagoya 464-8603, Japan; Institute of Materials and Systems for Sustainability (IMaSS), Nagoya University, Chikusa, Nagoya 464-8603, Japan; Department of Environmental Engineering and Architecture, Graduate School of Environmental Studies, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Electronic address: katayama.arata@nagoya-u.jp.
authors.forename: Arata
authors.initials: A
authors.lastname: Katayama
mesh_terms: D000693:Anaerobiosis; D001674:Bioelectric Energy Sources; D018441:Biofilms; D002384:Catalysis; D004560:Electricity; D004566:Electrodes; D045302:Geobacter / Q000235:genetics / Q000378:metabolism; D006108:Graphite / Q000378:metabolism*; D010084:Oxidation-Reduction; D019800:Phenol / Q000378:metabolism*; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D012336:RNA, Ribosomal, 16S; D019800:Phenol; D006108:Graphite
keywords: Biofilm; Geobacter; Graphite electrodes; Microbial fuel cell; Phenol degradation