title: Improvement in Liquid Chromatographic Performance of Organic Polymer Monolithic Capillary Columns with Controlled Free-Radical Polymerization.
abstract: Capillary columns containing butyl or lauryl methacrylate monoliths were prepared using two different free-radical polymerization methods: conventional free-radical polymerization and controlled/living free-radical polymerization, both initiated thermally, and these methods were compared for the first time. Both monolith morphology and chromatographic efficiency were compared for the synthesized stationary phases using scanning electronic microscopy (SEM) and capillary liquid chromatography, respectively. Columns prepared using controlled method gave better chromatographic performance for both monomers tested. The lauryl-based monolith showed 7-fold improvement in chromatographic efficiency with a plate count of 42,000 plates/m (corrected for dead volume) for a non-retained compound. Columns fabricated using controlled polymerization appeared more homogenous radially with fused small globular morphologies, evaluated by SEM, and lower column permeability. The columns were compared with respect to resolving power of a series of alkylbenzenes under isocratic and gradient elution conditions.
authors.affiliation: Institute of Chemistry, University of Campinas (Unicamp), POB 6154, 13084-971, Campinas, SP, Brazil.
authors.forename: Mariana R
authors.initials: MR
authors.lastname: Gama
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Pankaj
authors.initials: P
authors.lastname: Aggarwal
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Kun
authors.initials: K
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA.
authors.forename: Milton L
authors.initials: ML
authors.lastname: Lee
authors.affiliation: Institute of Chemistry, University of Campinas (Unicamp), POB 6154, 13084-971, Campinas, SP, Brazil.
authors.forename: Carla B G
authors.initials: CB
authors.lastname: Bottoli