title: Two new Cambodian semi-aquatic earthworms in the genusGlyphidrilusHorst, 1889 (Oligochaeta, Almidae), based on morphological and molecular data.
abstract: Combining morphological and molecular data is a powerful approach to support the discovery of new species. Here, two new species of the semi-aquatic earthworm genus Glyphidrilus, G. jamiesoni sp. n. and G. kralanhensis sp. n., are described from the Mekong Basin in Cambodia. They are morphologically distinguished by the respective locations of wings and spermathecae; furthermore, G. kralanhensis sp. n. has three pairs of ovaries, probably an autapomorphic trait. In addition, two mitochondrial gene fragments (COI and 16s rRNA) were sequenced of types of the new species and of five further Glyphidrilus species described recently from the Mekong basin in Thailand and Laos. They revealed a high level of genetic divergence of the new species compared to the other earthworm taxa. The evolutionary relationships among the Mekong Glyphidrilus members is discussed with reference to the recent paleogeography of the Mekong River drainage.
authors.affiliation: Biological Sciences Program, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand. Animal Systematics Research Unit, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.. parin_ohayo@hotmail.com.
authors.forename: Parin
authors.initials: P
authors.lastname: Jirapatrasilp
authors.forename: Pongpun
authors.initials: P
authors.lastname: Prasankok
authors.forename: Chirasak
authors.initials: C
authors.lastname: Sutcharit
authors.forename: Ratmanee
authors.initials: R
authors.lastname: Chanabun
authors.forename: Somsak
authors.initials: S
authors.lastname: Panha
mesh_terms: D063147:Animal Distribution; D000825:Animal Structures / Q000033:anatomy & histology / Q000254:growth & development; D000818:Animals; D049628:Body Size; D002160:Cambodia; D004272:DNA, Mitochondrial / Q000235:genetics; D017753:Ecosystem; D005260:Female; D008297:Male; D009835:Oligochaeta / Q000033:anatomy & histology* / Q000145:classification* / Q000254:growth & development; D009929:Organ Size; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004272:DNA, Mitochondrial; D012336:RNA, Ribosomal, 16S
keywords: Annelida, new species, earthworms, Glyphidrilus, Cambodia, integrative taxonomy, phylogeny, paleogeography