title: Lipid-coated hollow mesoporous silica nanospheres for co-delivery of doxorubicin and paclitaxel: Preparation, sustained release, cellular uptake and pharmacokinetics.
abstract: A carrier consisting of lipid-coated hollow mesoporous silica nanospheres (L-HMSN) was produced for the combination of the water-insoluble drug (paclitaxel, PTX) and the water-soluble drug (doxorubicin, DOX). DOX was adsorbed into the nanoscale hollow structure of the hollow mesoporous silica nanospheres (HMSN) by adsorption and PTX was wrapped in the phospholipid layer of the HMSN surface by lipid film hydration method. The characterization results showed that DOX and PTX were present in the nanopheres in an amorphous state. The loaded L-HMSN (DOX/PTX@L-HMSN) in vitro drug release showed a sustained release in phosphate buffered solution (PBS) at pH6.8 and 0.001%SDS. The cellular uptake experiment indicated that L-HMSN was successfully taken up by A549 cells. In addition, the combination of DOX and PTX in L-HMSN exhibited a marked synergistic effect in inhibiting the proliferation of A549 cells. The pharmacokinetic study demonstrated that L-HMSN could significantly improve the relative bioavailability of DOX and PTX. These results confirm that L-HMSN is a promising carrier for successful drug combination.
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Qiu
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China. Electronic address: wuchao27@126.com.
authors.forename: Chao
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Hao
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of pharmacy, Jinzhou Medical University, 40 Songpo Road, Linghe District, Jinzhou, Liaoning Province 121000, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ji
mesh_terms: D000072283:A549 Cells; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D002152:Calorimetry, Differential Scanning; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations; D004317:Doxorubicin / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D004705:Endocytosis; D005434:Flow Cytometry; D020128:Inhibitory Concentration 50; D008055:Lipids / Q000737:chemistry*; D054118:Nanospheres / Q000737:chemistry* / Q000648:ultrastructure; D017239:Paclitaxel / Q000493:pharmacokinetics*; D016062:Porosity; D011817:Rabbits; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D003692:Delayed-Action Preparations; D008055:Lipids; D012822:Silicon Dioxide; D004317:Doxorubicin; D017239:Paclitaxel
keywords: Doxorubicin; Drug combination; Lipid-coated hollow mesoporous silica nanospheres; Paclitaxel; Synergistic effect