title: Interleukin-1 Receptor 2: A New Biomarker for Sepsis Diagnosis and Gram-Negative/Gram-Positive Bacterial Differentiation.
abstract: This study was undertaken to explore the value of interleukin-1 receptor 2 (IL1R2) as a novel potential biomarker for diagnosis of sepsis and discrimination of gram-negative (G)/gram-positive (G) bacterial sepsis. The study was performed in Kunming mice and septic patients. Inactive Escherichia coli or Staphylococcus aureus were used to stimulate Kunming mice (10 CFU/kg). In clinical study, septic patients with different pathogen infection were studied, and healthy volunteers and patients with systemic inflammatory response syndrome without definite infection were enrolled as control. IL1R2 transcriptions of human subjects' peripheral leukocytes were measured by real-time quantitative polymerase chain reaction assay. IL1R2 serum concentrations of mice and human subjects were measured by enzyme-linked immunosorbent assay. The value of IL1R2 as a biomarker was compared with procalcitonin (PCT), C-reactive protein (CRP), and Acute Physiology and Chronic Health Evaluation II (APACHE II). The results showed that IL1R2 expression was upregulated in mice treated with inactive Escherichia coli and septic patients. The elevation of serum IL1R2 was more significant in septic patients infected by Escherichia coli or G bacteria than in those infected by Staphylococcus aureus or G bacteria. For sepsis diagnosis and G/G bacterial sepsis discrimination, serum IL1R2 was more sensitive and specific than the traditional biomarkers such as PCT, CRP, and APACHE II as shown by the receiver operating characteristic curves. It was suggested that IL1R2 was a potential biomarker for diagnosis and G/G bacterial differentiation in sepsis.
authors.affiliation: *Department of Pathophysiology, Xiangya School of Medicine, Central South University, Changsha, People's Republic of China †Translational Medicine Center of Sepsis, The Third Xiangya Hospital, Central South University, Changsha, People's Republic of China ‡Department of Critical Care Medicine, The Third Xiangya Hospital, Central South University, Changsha, People's Republic of China.
authors.forename: Yuejiao
authors.initials: Y
authors.lastname: Lang
authors.forename: Yu
authors.initials: Y
authors.lastname: Jiang
authors.forename: Min
authors.initials: M
authors.lastname: Gao
authors.forename: Wenmei
authors.initials: W
authors.lastname: Wang
authors.forename: Nian
authors.initials: N
authors.lastname: Wang
authors.forename: Kangkai
authors.initials: K
authors.lastname: Wang
authors.forename: Huali
authors.initials: H
authors.lastname: Zhang
authors.forename: Guangwen
authors.initials: G
authors.lastname: Chen
authors.forename: Ke
authors.initials: K
authors.lastname: Liu
authors.forename: Meidong
authors.initials: M
authors.lastname: Liu
authors.forename: Mingshi
authors.initials: M
authors.lastname: Yang
authors.forename: Xianzhong
authors.initials: X
authors.lastname: Xiao
mesh_terms: D000328:Adult; D000818:Animals; D015415:Biomarkers / Q000378:metabolism*; D004926:Escherichia coli / Q000472:pathogenicity; D006090:Gram-Negative Bacteria / Q000472:pathogenicity*; D006094:Gram-Positive Bacteria / Q000472:pathogenicity*; D006801:Humans; D008297:Male; D051379:Mice; D008875:Middle Aged; D053574:Receptors, Interleukin-1 Type II / Q000378:metabolism*; D018805:Sepsis / Q000378:metabolism* / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000472:pathogenicity
chemical_list: D015415:Biomarkers; C508592:IL1R2 protein, human; D053574:Receptors, Interleukin-1 Type II