title: MYBA2 gene involved in anthocyanin and flavonol biosynthesis pathways in grapevine.
abstract: MYBA2 transcription factor (Myb-related gene) affects the coloring in grapevine berry and plays an important role in the biosynthesis of anthocyanin. The MYBA2 gene was cloned from Vitis vinifera L. cv. Cabernet Sauvignon and polyclonal antibodies for VvmybA2 were prepared. The VvmybA2 gene expression patterns were observed in seven tissues (the leaf, stem, flower, bud, root, berry, and tendril) and during the berry development stage at transcriptional and translational levels, respectively. The results indicated that the expression of VvmybA2 was approximately 11-fold higher in the berry than that in the other six tissues, and increased rapidly from 60 days after full bloom reaching a maximum on day 80. Furthermore, both the anthocyanin content and UDP-glucose:flavonoid-3-O-glucosyltransferase (UFGT) gene expression levels increased rapidly 60 days after full bloom. Moreover, correlation analysis indicated that the transcriptional and translational expression levels of the VvmybA2 gene were significantly positively correlated with not only UFGT and DFR genes but also with the anthocyanin content during berry development. These results suggested that VvmybA2 could not only regulate the transcription of both UFGT and DFR but also is involved in the expression of the UFGT gene associated with color determination in grape berries.
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: T Q
authors.initials: TQ
authors.lastname: Niu
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: Z D
authors.initials: ZD
authors.lastname: Gao
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.|Key Laboratory of Pomology, Shanxi Province, China.
authors.forename: P F
authors.initials: PF
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.|Key Laboratory of Pomology, Shanxi Province, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: M Y
authors.initials: MY
authors.lastname: Gao
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China.|Key Laboratory of Pomology, Shanxi Province, China.
authors.forename: W
authors.initials: W
authors.lastname: Ji
authors.affiliation: Experimental Teaching Center, Shanxi Agricultural University, Taigu, Shanxi, China.
authors.forename: W X
authors.initials: WX
authors.lastname: Fan
authors.affiliation: College of Horticulture, Shanxi Agricultural University, Taigu, Shanxi, China wenpengfei@126.com.|Key Laboratory of Pomology, Shanxi Province, China wenpengfei@126.com.
authors.forename: P F
authors.initials: PF
authors.lastname: Wen
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics; D000872:Anthocyanins / Q000096:biosynthesis* / Q000235:genetics; D003001:Cloning, Molecular; D044948:Flavonols / Q000096:biosynthesis* / Q000235:genetics; D005638:Fruit / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D005964:Glucosyltransferases / Q000235:genetics; D009928:Organ Specificity; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000378:metabolism*
chemical_list: D000872:Anthocyanins; D044948:Flavonols; D010940:Plant Proteins; D014157:Transcription Factors; D000429:Alcohol Oxidoreductases; C056570:dihydroflavanol 4-reductase; D005964:Glucosyltransferases; C023249:flavonol 3-O-glucosyltransferase