title: Protective effects of β-sheet breaker α/β-hybrid peptide against amyloid β-induced neuronal apoptosis in vitro.
abstract: Alzheimer's disease is most common neurodegenerative disorder and is characterized by increased production of soluble amyloid-β oligomers, the main toxic species predominantly formed from aggregation of monomeric amyloid-β (Aβ). Increased production of Aβ invokes a cascade of oxidative damages to neurons and eventually leads to neuronal death. This study was aimed to investigate the neuroprotective effects of a β-sheet breaker α/β-hybrid peptide (BSBHp) and the underlying mechanisms against Aβ40-induced neurotoxicity in human neuroblastoma SH-SY5Y cells. Cells were pretreated with the peptide Aβ40to induce neurotoxicity. Assays for cell viability, cell membrane damage, cellular apoptosis, generation of reactive oxygen species (ROS), intracellular free Ca2+, and key apoptotic protein levels were performed in vitro. Our results showed that pretreatment with BSBHp significantly attenuates Aβ40-induced toxicity by retaining cell viability, suppressing generation of ROS, Ca2+levels, and effectively protects neuronal apoptosis by suppressing pro-apoptotic protein Bax and up-regulating antiapoptotic protein Bcl-2. These results suggest that α/β-hybrid peptide has neuroprotective effects against Aβ40-induced oxidative stress, which might be a potential therapeutic agent for treating or preventing neurodegenerative diseases.
authors.affiliation: Neuroscience Research Unit, Department of Physiology, Raja Peary Mohan College, Uttarpara, Hooghly, West Bengal, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Laboratory of Peptide and Amyloid Research, Department of Chemistry, Indian Institute of Technology Guwahati (IITG), North Guwahati, Assam, India.
authors.forename: Ashim
authors.initials: A
authors.lastname: Paul
authors.affiliation: Laboratory of Peptide and Amyloid Research, Department of Chemistry, Indian Institute of Technology Guwahati (IITG), North Guwahati, Assam, India.
authors.forename: Sourav
authors.initials: S
authors.lastname: Kalita
authors.affiliation: Department of Instrumentation Science, Jadavpur University, Kolkata, West Bengal, India.
authors.forename: Anup Kumar
authors.initials: AK
authors.lastname: Ghosh
authors.affiliation: Laboratory of Peptide and Amyloid Research, Department of Chemistry, Indian Institute of Technology Guwahati (IITG), North Guwahati, Assam, India.
authors.forename: Bhubaneswar
authors.initials: B
authors.lastname: Mandal
authors.affiliation: Neuroscience Research Unit, Department of Physiology, Raja Peary Mohan College, Uttarpara, Hooghly, West Bengal, India.|School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.
authors.forename: Amal Chandra
authors.initials: AC
authors.lastname: Mondal
mesh_terms: D016229:Amyloid beta-Peptides / Q000378:metabolism*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005786:Gene Expression Regulation / Q000187:drug effects; D019254:Genes, bcl-2 / Q000235:genetics; D006801:Humans; D046529:Microscopy, Electron, Transmission; D009474:Neurons / Q000187:drug effects*; D018696:Neuroprotective Agents / Q000737:chemistry / Q000494:pharmacology*; D010455:Peptides / Q000737:chemistry / Q000494:pharmacology*; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: D016229:Amyloid beta-Peptides; D018696:Neuroprotective Agents; D010455:Peptides; D051028:bcl-2-Associated X Protein
keywords: Alzheimer's disease; SH-SY5Y cell; amyloid-β peptide; apoptosis; human cerebrospinal fluid; neuroprotection; β-sheet breaker α/β-hybrid peptide