title: Isolation, identification and characterization of Cystobasidium oligophagum JRC1: A cellulase and lipase producing oleaginous yeast.
abstract: Oleaginous yeast closely related to Cystobasidium oligophagum was isolated from soil rich in cellulosic waste. The yeast was isolated based on its ability to accumulate intracellular lipid, grow on carboxymethylcellulose (CMC) and produce lipase. It could accumulate up to 39.44% lipid in a glucose medium (12.45±0.97g/l biomass production). It was able to grow and accumulate lipids (36.46%) in the medium containing CMC as the sole carbon source. The specific enzyme activities obtained for endoglucanase, exoglucanase, and β-glucosidase were 2.27, 1.26, and 0.98IU/mg respectively. The specific enzyme activities obtained for intracellular and extracellular lipase were 2.16 and 2.88IU/mg respectively. It could grow and accumulate lipids in substrates including glycerol (42.04%), starch (41.54%), xylose (36.24%), maltose (26.31%), fructose (24.29%), lactose (21.91%) and sucrose (21.72%). The lipid profile of the organism was suitable for obtaining biodiesel with desirable fuel properties.
authors.affiliation: Environmental Biotechnology Laboratory, Department of Biology, Indian Institute of Technology Jodhpur (IIT J), Jodhpur, Rajasthan 342011, India.
authors.forename: Sachin
authors.initials: S
authors.lastname: Vyas
authors.affiliation: Environmental Biotechnology Laboratory, Department of Biology, Indian Institute of Technology Jodhpur (IIT J), Jodhpur, Rajasthan 342011, India. Electronic address: meenuchhabra@iitj.ac.in.
authors.forename: Meenu
authors.initials: M
authors.lastname: Chhabra
mesh_terms: D056804:Biofuels; D002480:Cellulase; D005227:Fatty Acids; D008049:Lipase; D008055:Lipids
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D008049:Lipase; D002480:Cellulase
keywords: Cellulase; Cellulolytic yeast; Cystobasidium oligophagum; FAME; Lipase; Oleaginous yeast; Rhodotorula oligophaga