title: Chromosome-Encoded Broad-Spectrum Ambler Class A β-Lactamase RUB-1 from Serratia rubidaea.
abstract: Whole-genome sequencing of Serratia rubidaea CIP 103234Trevealed a chromosomally located Ambler class A β-lactamase gene. The gene was cloned, and the β-lactamase, RUB-1, was characterized. RUB-1 displayed 74% and 73% amino acid sequence identity with the GIL-1 and TEM-1 penicillinases, respectively, and its substrate profile was similar to that of the latter β-lactamases. Analysis by 5' rapid amplification of cDNA ends revealed promoter sequences highly divergent from the Escherichia coli σ70consensus sequence. This work further illustrates the heterogeneity of β-lactamases among Serratia spp.
authors.affiliation: EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France.|French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France.|Joint Research Unit EERA "Evolution and Ecology of Resistance to Antibiotics," Institut Pasteur-APHP-Université Paris Sud, Paris, France.
authors.forename: Rémy A
authors.identifier: 0000-0002-2307-3232
authors.initials: RA
authors.lastname: Bonnin
authors.affiliation: EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Didi
authors.affiliation: EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France.
authors.forename: Ayla
authors.initials: A
authors.lastname: Ergani
authors.affiliation: EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: Lima
authors.affiliation: EA7361 "Structure, Dynamic, Function and Expression of Broad Spectrum β-Lactamases," Université Paris Sud, Université Paris Saclay, LabEx Lermit, Faculty of Medicine, Le Kremlin-Bicêtre, France thierry.naas@aphp.fr.|French National Reference Center for Antibiotic Resistance: Carbapenemase-Producing Enterobacteriaceae, Le Kremlin-Bicêtre, France.|Joint Research Unit EERA "Evolution and Ecology of Resistance to Antibiotics," Institut Pasteur-APHP-Université Paris Sud, Paris, France.|Bactériologie-Hygiene Unit, APHP, Bicêtre Hospital, Le Kremlin-Bicêtre, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Naas
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002876:Chromosomes, Bacterial / Q000235:genetics*; D018076:DNA, Complementary / Q000235:genetics; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology / Q000378:metabolism; D008826:Microbial Sensitivity Tests; D010405:Penicillinase / Q000235:genetics / Q000378:metabolism; D012705:Serratia / Q000187:drug effects / Q000201:enzymology* / Q000378:metabolism*; D001618:beta-Lactamases / Q000235:genetics / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D018076:DNA, Complementary; D010405:Penicillinase; D001618:beta-Lactamases
keywords: RUB-1; Serratia rubidaea; class A; β-lactamase