title: The thalamic mGluR1-PLCβ4 pathway is critical in sleep architecture.
abstract: The transition from wakefulness to a nonrapid eye movement (NREM) sleep state at the onset of sleep involves a transition from low-voltage, high-frequency irregular electroencephalography (EEG) waveforms to large-amplitude, low-frequency EEG waveforms accompanying synchronized oscillatory activity in the thalamocortical circuit. The thalamocortical circuit consists of reciprocal connections between the thalamus and cortex. The cortex sends strong excitatory feedback to the thalamus, however the function of which is unclear. In this study, we investigated the role of the thalamic metabotropic glutamate receptor 1 (mGluR1)-phospholipase C β4 (PLCβ4) pathway in sleep control in PLCβ4-deficient (PLCβ4-/-) mice. The thalamic mGluR1-PLCβ4 pathway contains synapses that receive corticothalamic inputs. In PLCβ4-/-mice, the transition from wakefulness to the NREM sleep state was stimulated, and the NREM sleep state was stabilized, which resulted in increased NREM sleep. The power density of delta (δ) waves increased in parallel with the increased NREM sleep. These sleep phenotypes in PLCβ4-/-mice were consistent in TC-restricted PLCβ4 knockdown mice. Moreover, in vitro intrathalamic oscillations were greatly enhanced in the PLCβ4-/-slices. The results of our study showed that thalamic mGluR1-PLCβ4 pathway was critical in controlling sleep architecture.
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea.
authors.forename: Joohyeon
authors.initials: J
authors.lastname: Hong
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Daejeon, Republic of Korea.
authors.forename: Jungryun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea.
authors.forename: Kiyeong
authors.initials: K
authors.lastname: Song
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea.
authors.forename: Go Eun
authors.initials: GE
authors.lastname: Ha
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea.
authors.forename: Yong Ryoul
authors.initials: YR
authors.lastname: Yang
authors.affiliation: Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Ji Su
authors.initials: JS
authors.lastname: Ma
authors.affiliation: Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Daejeon, Republic of Korea.
authors.forename: Hee-Sup
authors.initials: HS
authors.lastname: Shin
authors.affiliation: School of Life Sciences, Ulsan National Institute of Science and Technology, Ulsan, Republic of Korea. pgsuh@unist.ac.kr.
authors.forename: Pann-Ghill
authors.initials: PG
authors.lastname: Suh
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul, Republic of Korea. eunjicheong@yonsei.ac.kr.
authors.forename: Eunji
authors.identifier: 0000-0001-6158-7851
authors.initials: E
authors.lastname: Cheong
mesh_terms: D000818:Animals; D002540:Cerebral Cortex / Q000502:physiology; D003700:Delta Rhythm / Q000502:physiology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D054799:Phospholipase C beta / Q000172:deficiency / Q000378:metabolism*; D018094:Receptors, Metabotropic Glutamate / Q000378:metabolism*; D012890:Sleep / Q000502:physiology*; D013788:Thalamus / Q000378:metabolism* / Q000502:physiology
chemical_list: D018094:Receptors, Metabotropic Glutamate; C104077:metabotropic glutamate receptor type 1; D054799:Phospholipase C beta
keywords: Delta wave; Knockout mice; Phospholipase C β4; Sleep; Thalamocortical oscillation; Thalamus