title: Deletion of the sclerotome-enriched lncRNA PEAT augments ribosomal protein expression.
abstract: To define a complete catalog of the genes that are activated during mouse sclerotome formation, we sequenced RNA from embryonic mouse tissue directed to form sclerotome in culture. In addition to well-known early markers of sclerotome, such as Pax1, Pax9, and the Bapx2/Nkx3-2 homolog Nkx3-1, the long-noncoding RNA PEAT (Pax1 enhancer antisense transcript) was induced in sclerotome-directed samples. Strikingly, PEAT is located just upstream of the Pax1 gene. Using CRISPR/Cas9, we generated a mouse line bearing a complete deletion of the PEAT-transcribed unit. RNA-seq on PEAT mutant embryos showed that loss of PEAT modestly increases bone morphogenetic protein target gene expression and also elevates the expression of a large subset of ribosomal protein mRNAs.
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720.
authors.forename: David A
authors.initials: DA
authors.lastname: Stafford
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720.
authors.forename: Darwin S
authors.initials: DS
authors.lastname: Dichmann
authors.affiliation: Department of Genetics, Stanford University, Stanford, CA 94305-5120.
authors.forename: Jessica K
authors.initials: JK
authors.lastname: Chang
authors.affiliation: Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720; harland@berkeley.edu.
authors.forename: Richard M
authors.initials: RM
authors.lastname: Harland
mesh_terms: D000818:Animals; D019485:Bone Morphogenetic Proteins / Q000096:biosynthesis; D064113:CRISPR-Cas Systems / Q000235:genetics; D047108:Embryonic Development / Q000235:genetics*; D018507:Gene Expression Regulation, Developmental / Q000235:genetics*; D008648:Mesoderm / Q000196:embryology; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051761:Paired Box Transcription Factors / Q000096:biosynthesis / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D012335:RNA, Ribosomal / Q000096:biosynthesis*; D012269:Ribosomal Proteins / Q000096:biosynthesis* / Q000235:genetics; D017384:Sequence Deletion / Q000235:genetics
chemical_list: D019485:Bone Morphogenetic Proteins; D051761:Paired Box Transcription Factors; D062085:RNA, Long Noncoding; D012335:RNA, Ribosomal; D012269:Ribosomal Proteins; C070835:PAX1 transcription factor
keywords: BMP pathway; CRISPR/Cas9; long-noncoding RNA; ribosomal proteins; sclerotome