title: Efficient derivation of extraembryonic endoderm stem cell lines from mouse postimplantation embryos.
abstract: Various types of stem cell lines have been derived from preimplantation or postimplantation mouse embryos: embryonic stem cell lines, epiblast stem cell lines, and trophoblast stem cell lines. It is not known if extraembryonic endoderm stem (XEN) cell lines can be derived from postimplantation mouse embryos. Here, we report the derivation of 77 XEN cell lines from 85 postimplantation embryos at embryonic day E5.5 or E6.5, in parallel to the derivation of 41 XEN lines from 69 preimplantation embryos at the blastocyst stage. We attain a success rate of 100% of XEN cell line derivation with our E5.5 whole-embryo and E6.5 disaggregated-embryo methods. Immunofluorescence and NanoString gene expression analyses indicate that the XEN cell lines that we derived from postimplantation embryos (post-XEN) are very similar to the XEN cell lines that we derived from preimplantation embryos (pre-XEN) using a conventional method. After injection into blastocysts, post-XEN cells contribute to extraembryonic endoderm in chimeras at E6.5 and E7.5.
authors.affiliation: Max Planck Research Unit for Neurogenetics, Max-von-Laue-Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Lin
authors.affiliation: Max Planck Research Unit for Neurogenetics, Max-von-Laue-Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Mona
authors.initials: M
authors.lastname: Khan
authors.affiliation: Max Planck Research Unit for Neurogenetics, Max-von-Laue-Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Bolek
authors.initials: B
authors.lastname: Zapiec
authors.affiliation: Max Planck Research Unit for Neurogenetics, Max-von-Laue-Strasse 4, 60438 Frankfurt, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Mombaerts
mesh_terms: D000818:Animals; D001755:Blastocyst; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D003470:Culture Media / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology*; D004707:Endoderm / Q000166:cytology*; D005260:Female; D020869:Gene Expression Profiling; D005855:Germ Layers / Q000166:cytology; D049452:Green Fluorescent Proteins / Q000378:metabolism; D008297:Male; D051379:Mice; D008856:Microscopy, Fluorescence; D014327:Trophoblasts / Q000166:cytology
chemical_list: D003470:Culture Media; D049452:Green Fluorescent Proteins