title: The binuclear form of dinitrosyl iron complexes with thiol-containing ligands in animal tissues.
abstract: It has been established that treatment of mice with sodium nitrite, S-nitrosoglutathione and the water-soluble nitroglycerine derivative isosorbide dinitrate (ISDN) as NO donors initiates in vivo synthesis of significant amounts of EPR-silent binuclear dinitrosyl iron complexes (B-DNIC) with thiol-containing ligands in the liver and other tissues of experimental mice. This effect is especially apparent if NO donors are administered to mice simultaneously with the Fe2+-citrate complex. Similar results were obtained in experiments on isolated liver and other mouse tissues treated with gaseous NО in vitro and during stimulation of endogenous NO synthesis in the presence of inducible NO synthase. B-DNIC appeared in mouse tissues after in vitro treatment of tissue samples with an aqueous solution of diethyldithiocarbamate (DETC), which resulted in the transfer of iron-mononitrosyl fragments from B-DNIC to the thiocarbonyl group of DETC and the formation of EPR-detectable mononitrosyl iron complexes (MNIC) with DETC. EPR-Active MNIC with N-methyl-d-glucamine dithiocarbamate (MGD) were synthesized in a similar way. MNIC-MGD were also formed in the reaction of water-soluble MGD-Fe2+complexes with sodium nitrite, S-nitrosoglutathione and ISDN.
authors.affiliation: N.N.Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Vasak D
authors.initials: VD
authors.lastname: Mikoyan
authors.affiliation: N.N.Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Evgeniya N
authors.initials: EN
authors.lastname: Burgova
authors.affiliation: N.N.Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: Rostislav R
authors.initials: RR
authors.lastname: Borodulin
authors.affiliation: N.N.Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia; Institute for Regenerative Medicine, I.M.Sechenov Moscow State Medical University, Moscow, Russia. Electronic address: vanin.dnic@gmail.com.
authors.forename: Anatoly F
authors.initials: AF
authors.lastname: Vanin
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry / Q000378:metabolism; D000818:Animals; D004050:Ditiocarb / Q000737:chemistry / Q000378:metabolism*; D005296:Ferrous Compounds / Q000737:chemistry / Q000378:metabolism*; D005978:Glutathione / Q000737:chemistry / Q000378:metabolism; D006454:Hemoglobins / Q000378:metabolism; D007548:Isosorbide Dinitrate / Q000737:chemistry; D008024:Ligands; D008070:Lipopolysaccharides / Q000494:pharmacology; D008297:Male; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009573:Nitrites / Q000737:chemistry / Q000378:metabolism; D026422:S-Nitrosoglutathione / Q000737:chemistry / Q000378:metabolism; D013012:Sorbitol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000378:metabolism; D013113:Spin Labels; D013859:Thiocarbamates / Q000737:chemistry / Q000378:metabolism*
chemical_list: D005296:Ferrous Compounds; D006454:Hemoglobins; D008024:Ligands; D008070:Lipopolysaccharides; D009573:Nitrites; D013113:Spin Labels; D013859:Thiocarbamates; C008657:nitrosyl hemoglobin; D009569:Nitric Oxide; D013012:Sorbitol; D026422:S-Nitrosoglutathione; C041262:N-methyl-D-glucamine dithiocarbamate; D004050:Ditiocarb; D052247:Nitric Oxide Synthase Type II; C496317:Nos2 protein, mouse; D005978:Glutathione; D007548:Isosorbide Dinitrate; D000111:Acetylcysteine
keywords: Dinitrosyl iron complexes; Nitric oxide; S-nitrosothiols