title: Fine mapping and candidate gene analysis of Brtri1, a gene controlling trichome development in Chinese cabbage (Brassica rapa L. ssp pekinensis).
abstract: Trichomes are derived from the epidermis and constitute an ideal system for studying cell division in plants. Here, a Chinese cabbage doubled haploid (DH) line (FT) without trichomes was crossed with another DH line (PurDH-1) with trichomes to develop an F2population for fine mapping of trichome control genes. Genetic analysis showed that the trichome phenotype was controlled by a single dominant gene, Brtri1. Using 1226 glabrous individuals in the F2segregation population, Brtri1 was localized to a 16.84 kb region between markers Pur6-31 and Pur6-39 on chromosome A06. One of the four complete open reading frames within the mapping region, Bra025311, encodes a MYB transcription factor and is highly homologous to the trichome regulatory gene GL1 in Arabidopsis thaliana. It was thus regarded as a candidate gene for Brtri1. Comparative sequencing showed a 5-bp deletion in the third exon of Bra025311 in FT, resulting in a frame-shift mutation. No expression of Bra025311 was detected in FT. A co-dominant indel marker close to this mutation site was developed for marker-assisted selection in Chinese cabbage breeding.
authors.forename: X L
authors.initials: XL
authors.lastname: Ye
authors.forename: F Y
authors.initials: FY
authors.lastname: Hu
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: J
authors.initials: J
authors.lastname: Ren
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: S N
authors.initials: SN
authors.lastname: Huang
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: W J
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: H
authors.initials: H
authors.lastname: Feng
authors.affiliation: Department of Horticulture, Shenyang Agricultural University, Shenyang, China.
authors.forename: Z Y
authors.initials: ZY
authors.lastname: Liu
mesh_terms: D029741:Brassica rapa / Q000235:genetics* / Q000254:growth & development; D002874:Chromosome Mapping / Q000379:methods*; D003433:Crosses, Genetic; D018744:DNA, Plant / Q000032:analysis; D016368:Frameshift Mutation; D005819:Genetic Markers; D006238:Haploidy; D010641:Phenotype; D017422:Sequence Analysis, DNA; D014157:Transcription Factors / Q000235:genetics*; D064201:Trichomes / Q000235:genetics / Q000254:growth & development*
chemical_list: D018744:DNA, Plant; D005819:Genetic Markers; D014157:Transcription Factors