title: Functional food applications of dextran from Weissella cibaria RBA12 from pummelo (Citrus maxima).
abstract: Weissella cibaria RBA12 isolated from pummelo from Northeast India produces a dextran composed of 97% α-(1→6) linkages in the main chain and 3% α-(1→3) branched linkages. The in vitro prebiotic activity of dextran-RBA12 was explored. Dextran-RBA12 displayed enhanced growth of probiotic Bifidobacterium and Lactobacillus spp., and controlled growth of non-probiotic enteric bacteria. Dextran-RBA12 showed superior resistance to physiological barriers with a maximum hydrolysis of 0.51%, 0.31% and 0.24% by artificial gastric juice, α-amylase and intestinal fluid, respectively, whereas compared to maximum hydrolysis of 25.23%, 19.13% and 6%, respectively after 5h of incubation shown by commercial prebiotic inulin. The production of dextran from Weissella cibaria RBA12 in sourdough prepared from whole wheat flour, wheat bran and rye bran showed the highest dextran of 3.26±0.12% d.w. in rye bran. The overall study summarized that dextran-RBA12 can be used as a prebiotic and also can be easily produced in sourdough.
authors.affiliation: Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
authors.forename: Rwivoo
authors.initials: R
authors.lastname: Baruah
authors.affiliation: Department of Food and Environmental Sciences, P.O. Box 27, FI-00014, University of Helsinki, Finland.
authors.forename: Ndegwa H
authors.initials: NH
authors.lastname: Maina
authors.affiliation: Department of Food and Environmental Sciences, P.O. Box 27, FI-00014, University of Helsinki, Finland.
authors.forename: Kati
authors.initials: K
authors.lastname: Katina
authors.affiliation: VTT Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT Espoo, Finland.
authors.forename: Riikka
authors.initials: R
authors.lastname: Juvonen
authors.affiliation: Carbohydrate Enzyme Biotechnology Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India. Electronic address: arungoyl@iitg.ernet.in.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
mesh_terms: D001644:Bifidobacterium / Q000254:growth & development / Q000378:metabolism; D002957:Citrus / Q000382:microbiology*; D003911:Dextrans / Q000032:analysis / Q000378:metabolism*; D004043:Dietary Fiber / Q000378:metabolism / Q000382:microbiology; D005433:Flour / Q000032:analysis / Q000382:microbiology; D055951:Functional Food / Q000032:analysis*; D007194:India; D056692:Prebiotics / Q000032:analysis*; D014908:Triticum / Q000382:microbiology; D058836:Weissella / Q000737:chemistry* / Q000378:metabolism
chemical_list: D003911:Dextrans; D004043:Dietary Fiber; D056692:Prebiotics
keywords: Dextran; Inulin; NMR; Prebiotic; Sourdough; Weissella