title: Evaluation of a candidate live attenuated influenza vaccine prepared in Changchun BCHT (China) for safety and efficacy in ferrets.
abstract: We evaluated the safety and efficacy of a live attenuated influenza vaccine (LAIV) product in ferrets. The BCHT LAIV product was significantly less virulent than wild-type H1N1 virus, when evaluated by comparing virus shedding and histopathologic lesions. The data indicated strong evidence for an attenuated phenotype of LAIV. Furthermore, the vaccine induced robust humoral immune responses in seronegative ferrets, and protected ferrets against development of fever, weight loss and turbinate inflammatory lesions after challenging with H3N2 wide-type influenza virus. Thus, the BCHT LAIV product was safe in healthy seronegative ferrets and protected ferrets against infection of H3N2 influenza virus.
authors.affiliation: Changchun BCHT Biotechnology Co., Changchun, PR China.
authors.forename: Zhenwei
authors.initials: Z
authors.lastname: Shen
authors.affiliation: Department of Biomedical Sciences, Colorado State University, Fort Collins, United States.
authors.forename: Richard A
authors.initials: RA
authors.lastname: Bowen
authors.affiliation: Changchun BCHT Biotechnology Co., Changchun, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ge
authors.affiliation: Changchun BCHT Biotechnology Co., Changchun, PR China.
authors.forename: Jinfei
authors.initials: J
authors.lastname: Yu
authors.affiliation: Changchun BCHT Biotechnology Co., Changchun, PR China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Shen
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.
authors.forename: Chunlai
authors.initials: C
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China.
authors.forename: Jinchang
authors.initials: J
authors.lastname: Wu
authors.affiliation: Changchun BCHT Biotechnology Co., Changchun, PR China.
authors.forename: Changlin
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Changchun BCHT Biotechnology Co., Changchun, PR China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Changchun BCHT Biotechnology Co., Changchun, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Virology, Institute of Experimental Medicine, 12 Acad. Pavlov Street, Saint Petersburg 197376, Russia.
authors.forename: Larisa
authors.initials: L
authors.lastname: Rudenko
authors.affiliation: Department of Virology, Institute of Experimental Medicine, 12 Acad. Pavlov Street, Saint Petersburg 197376, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Kiseleva
authors.affiliation: School of Life Sciences, Jilin University, Changchun, PR China. Electronic address: feixu@jlu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood; D002681:China; D005289:Ferrets; D006801:Humans; D056724:Immunity, Humoral; D053118:Influenza A Virus, H1N1 Subtype / Q000276:immunology / Q000302:isolation & purification / Q000472:pathogenicity; D053122:Influenza A Virus, H3N2 Subtype / Q000276:immunology* / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology; D009981:Influenza B virus / Q000276:immunology / Q000302:isolation & purification; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000009:adverse effects* / Q000276:immunology*; D007251:Influenza, Human / Q000517:prevention & control; D008297:Male; D009976:Orthomyxoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D014613:Vaccines, Attenuated / Q000008:administration & dosage / Q000009:adverse effects / Q000276:immunology; D017201:Virus Shedding
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; D014613:Vaccines, Attenuated
keywords: Ferret; Influenza; Trivalent live attenuated influenza vaccine