title: The effect of RGD peptide on 2D and miniaturized 3D culture of HEPM cells, MSCs, and ADSCs with alginate hydrogel.
abstract: Advancements in tissue engineering require the development of new technologies to study cell behaviorin vitro. This study focuses on stem cell behavior within various miniaturized three-dimensional (3D) culture conditions of alginate biomaterials modified with the Arg-Gly-Asp (RGD) peptide known for its role in cell adhesion/attachment. Human embryonic palatal mesenchyme (HEPM) cells, bone marrow derived mesenchymal stem cells (MSCs), and human adipose derived stem cells (ADSCs) were cultured on a flat hydrogel of different concentrations of alginate-RGD, and in the miniaturized 3D core of microcapsules with either a 2% alginate or 2% alginate-RGD shell. The core was made of 0%, 0.5%, or 2% alginate-RGD. Cell spreading was observed in all systems containing the RGD peptide, and the cell morphology was quantified by measuring the cell surface area and circularity. In all types of stem cells, there was a significant increase in the cell surface area (p< 0.05) and a significant decrease in cell circularity (p< 0.01) in alginate-RGD conditions, indicating that cells spread much more readily in environments containing the peptide. This control over the cell spreading within a 3D microenvironment can help to create the ideal biomimetic condition in which to conduct further studies on cell behavior.
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA).
authors.forename: Jenna
authors.initials: J
authors.lastname: Dumbleton
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA).
authors.forename: Pranay
authors.initials: P
authors.lastname: Agarwal
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA); Department of Mechanical and Aerospace Engineering, The Ohio State University, Columbus, OH 43210 (USA).
authors.forename: Haishui
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA).
authors.forename: Nathaniel
authors.initials: N
authors.lastname: Hogrebe
authors.affiliation: Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA); Department of Surgery, The Ohio State University, Columbus, OH 43210 (USA).
authors.forename: Renzhi
authors.initials: R
authors.lastname: Han
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA).
authors.forename: Keith J
authors.initials: KJ
authors.lastname: Gooch
authors.affiliation: Department of Biomedical Engineering, The Ohio State University, Columbus, OH 43210 (USA); Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University, Columbus, OH 43210 (USA); Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210 (USA).
authors.forename: Xiaoming
authors.initials: X
authors.lastname: He
keywords: 3D culture; Core-shell microcapsules; biomimetic; stem cell