title: Oral acute toxicity study as well as tissues oxidative stress and histopathological disorders in edible camphor administered rats.
abstract: In the South-western part of Nigeria, edible camphor (EC) infusions are used to treat pile, back pain, and erectile dysfunction, especially in preparation for sexual intercourse. We therefore carried out oral acute toxicity study, and then investigated the effects of various doses of EC on the activities of lactate dehydrogenase (LDH), catalase (CAT), and superoxide dismutase (SOD), as well as reduced glutathione (GSH) and malondialdehyde (MDA) levels in wistar rats. Oral LD50of EC was estimated to be 9487mg/kg body weight. Based on this, thirty animals were divided into six groups of five rats each, and were orally administered various doses of EC (1, 2, 4, and 6g/kg body weight) for seven days. Comparing all results with control, EC significantly increased serum LDH activity (4 and 6g/kg), liver (6g/kg) and kidney (4 and 6g/kg) MDA levels, as well testis GSH levels (1g/kg). CAT activities were significantly decreased in liver, kidney, and testis, and also lung GSH level by all the tested doses. For SOD, activities were significantly increased in liver and lung, but significantly decreased in kidney (2, 4, and 6g/kg). Various pathological disorders were also seen following the various doses of EC administered, especially in liver, kidney and lung. Therefore, from our findings, it is evident that incessant, misuse or overconsumption of EC could lead to oxidative tissue damage in rats.
authors.affiliation: Department of Biochemistry, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria. Electronic address: toblerum@yahoo.co.uk.
authors.forename: Oluwatobi T
authors.initials: OT
authors.lastname: Somade
authors.affiliation: Department of Biochemistry, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria.
authors.forename: Kafilat D
authors.initials: KD
authors.lastname: Adeniji
authors.affiliation: Department of Biochemistry, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria.
authors.forename: Abdul-Rahman A
authors.initials: AA
authors.lastname: Adesina
authors.affiliation: Department of Biochemistry, College of Biosciences, Federal University of Agriculture Abeokuta, Abeokuta, Nigeria.
authors.forename: Oluremi J
authors.initials: OJ
authors.lastname: Olurinde
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002164:Camphor / Q000008:administration & dosage / Q000633:toxicity*; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D007668:Kidney / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D008168:Lung / Q000187:drug effects; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D051381:Rats; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects
chemical_list: D002164:Camphor
keywords: Acute toxicity; Edible camphor; Histopathology; Oral; Oxidative stress