title: Human dental pulp stem cells expressing STRO-1, c-kit and CD34 markers in peripheral nerve regeneration.
abstract: Peripheral nerve injuries are a commonly encountered clinical problem and often result in long-term functional defects. The application of stem cells able to differentiate in Schwann cell-like cells in vitro and in vivo, could represent an attractive therapeutic approach for the treatment of nerve injuries. Further, stem cells sources sharing the same embryological origin as Schwann cells might be considered a suitable tool. The aim of this study was to demonstrate the ability of a neuroectodermal subpopulation of human STRO-1+/c-Kit+/CD34+DPSCs, expressing P75NTR, nestin and SOX-10, to differentiate into Schwann cell-like cells in vitro and to promote axonal regeneration in vivo, which led to functional recovery as measured by sustained gait improvement, in animal rat model of peripheral nerve injury. Transplanted human dental pulp stem cells (hDPSCs) engrafted into sciatic nerve defect, as revealed by the positive staining against human nuclei, showed the expression of typical Schwann cells markers, S100b and, noteworthy, a significant number of myelinated axons was detected. Moreover, hDPSCs promoted axonal regeneration from proximal to distal stumps 1 month after transplantation. This study demonstrates that STRO-1+/c-Kit+/CD34+hDPSCs, associated with neural crest derivation, represent a promising source of stem cells for the treatment of demyelinating disorders and might provide a valid alternative tool for future clinical applications to achieve functional recovery after injury or peripheral neuropathies besides minimizing ethical issues. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Gianluca
authors.initials: G
authors.lastname: Carnevale
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pisciotta
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Riccio
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: Bertoni
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: De Biasi
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Lara
authors.initials: L
authors.lastname: Gibellini
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Alessio
authors.initials: A
authors.lastname: Zordani
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Gian Maria
authors.initials: GM
authors.lastname: Cavallini
authors.affiliation: Department of Obstetrics and Gynecology, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Giovanni Battista
authors.initials: GB
authors.lastname: La Sala
authors.affiliation: Oro-Maxillo-Facial Department, AUSL Baggiovara, Baggiovara, Modena, Italy.
authors.forename: Giacomo
authors.initials: G
authors.lastname: Bruzzesi
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.|Children Rehabilitation Special Unit, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Ferrari
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cossarizza
authors.affiliation: Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Modena, Italy.
authors.forename: Anto
authors.initials: A
authors.lastname: de Pol
mesh_terms: D000328:Adult; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D015415:Biomarkers / Q000378:metabolism*; D002454:Cell Differentiation; D060833:Cellular Microenvironment; D003782:Dental Pulp / Q000166:cytology*; D006801:Humans; D039902:Multipotent Stem Cells / Q000166:cytology; D009186:Myelin Sheath / Q000378:metabolism; D009414:Nerve Growth Factors / Q000378:metabolism; D009416:Nerve Regeneration; D009432:Neural Crest / Q000378:metabolism; D010525:Peripheral Nerves / Q000502:physiology*; D010641:Phenotype; D019009:Proto-Oncogene Proteins c-kit / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function; D012584:Sciatic Nerve / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D013234:Stem Cells / Q000378:metabolism*; D055815:Young Adult
chemical_list: D018952:Antigens, CD34; D015415:Biomarkers; D009414:Nerve Growth Factors; D019009:Proto-Oncogene Proteins c-kit
keywords: STRO-1+/c-Kit+/CD34+; collagen scaffold; ectomesenchyme; human DPSCs; nestin; neural crest; peripheral nerve regeneration; remyelination