title: Characterization of South American Snails of the GenusBiomphalaria(Basommatophora: Planorbidae) andSchistosoma mansoni(Platyhelminthes: Trematoda) in Molluscs by PCR-RFLP.
abstract: The identification of snails of the genusBiomphalariacan be done using morphological characteristics which depends on the size of the snails and skill and knowledge of researcher. These methods sometimes are not adequate for identification of species. The PCR-RFLP, using the ITS region of the rDNA, has been used to identify Brazilian species of the genusBiomphalaria. Nevertheless, there is a lack of information about snails from other Latin American countries. In addition, some snails may be infected bySchistosoma mansoniand when submitted to PCR-RFLP they show molecular profiles different from those previously standardized for the other mollusc species. In this work the molecular profiles of 15 species and the subspecies were established by PCR-RFLP of ITS-rDNA with the enzymeDdeI. Moreover, the molecular profiles of host species,B. glabrata,B. straminea,B. tenagophila, andB. prona, infected byS. mansoniwere also established. The molluscs were dissected to permit morphological identification. These results contribute to a correct identification of snails of the genusBiomphalariaand detection of these snails infected byS. mansoni.
authors.affiliation: Grupo de Pesquisa em Helmintologia e Malacologia Médica, Centro de Pesquisas René Rachou, Fiocruz, Avenida Augusto de Lima 1715, 30190-001 Belo Horizonte, MG, Brazil.
authors.forename: Roberta Lima
authors.identifier: 0000-0003-4706-0732
authors.initials: RL
authors.lastname: Caldeira
authors.affiliation: Biociências e Biotecnologia em Saúde, Departamento de Entomologia, Centro de Pesquisas Aggeu Magalhães (CPqAm), Recife, PE, Brazil.
authors.forename: Tatiana Maria
authors.initials: TM
authors.lastname: Teodoro
authors.affiliation: Moluscário "Lobato Paraense" Centro de Pesquisas René Rachou, Fiocruz, Avenida Augusto de Lima 1715, 30190-001 Belo Horizonte, MG, Brazil.
authors.forename: Liana Konovaloff
authors.initials: LK
authors.lastname: Jannotti-Passos
authors.affiliation: Prefeitura Municipal de Belo Horizonte, Av. Afonso Pena 1212, 30130-003 Belo Horizonte, MG, Brazil.
authors.forename: Pollanah M
authors.initials: PM
authors.lastname: Lira-Moreira
authors.affiliation: Sessão de Parasitologia, Laboratório de Parasitoses Intestinais e Malacologia, Instituto Evandro Chagas, Rodovia BR-316 Km 7 s/n, Levilândia, 67030-000 Ananindeua, PA, Brazil.
authors.forename: Christiane De Oliveira
authors.initials: CO
authors.lastname: Goveia
authors.affiliation: Grupo de Pesquisa em Helmintologia e Malacologia Médica, Centro de Pesquisas René Rachou, Fiocruz, Avenida Augusto de Lima 1715, 30190-001 Belo Horizonte, MG, Brazil.
authors.forename: Omar Dos Santos
authors.initials: OD
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D001700:Biomphalaria / Q000469:parasitology*; D018509:DNA, Helminth / Q000235:genetics*; D004275:DNA, Ribosomal / Q000235:genetics*; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012550:Schistosoma mansoni / Q000235:genetics*; D013020:South America
chemical_list: D018509:DNA, Helminth; D004275:DNA, Ribosomal