title: Annexin A3 Knockdown Suppresses Lung Adenocarcinoma.
abstract: Our previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis. However, the biological functions of Anxa3 in LADC are not fully clear. In this study,in vitroandin vivoassays were performed to investigate the effects of Anxa3 downregulation on the growth, migration, invasion, metastasis, and signaling pathway activation of LADC cells. After Anxa3 downregulation, the growth of A549 and LTEP-a2 LADC cells was slowed and they showed decreased migration and invasionin vitro. Anxa3 knockdown significantly inhibited tumor formation by A549 cellsin vivo; while many metastases were formed by control A549 cells, there were obvious reductions in the numbers of lung, liver, and brain metastases formed by Anxa3 knockdown in A549 cells. Furthermore, Anxa3 knockdown significantly decreased MMP-2 and N-cadherin expression and increased E-cadherin expression both in cell linesin vitroand in tumor nodules examined duringin vivotumorigenesis assays. Interestingly, Anxa3 downregulation reduced the phosphorylated levels of MEK and ERK. In summary, Anxa3 knockdown inhibited the growth, migration, invasion, and metastasis of LADC, decreased the activation of the MEK/ERK signaling pathway, and modulated the expression of MMP-2, E-cadherin, and N-cadherin.
authors.affiliation: Department of Basic Medical Sciences, Medical College, Xiamen University, Xiamen, Fujian, China.
authors.forename: Ying-Fu
authors.identifier: 0000-0003-1626-4062
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Department of Basic Medical Sciences, Medical College, Xiamen University, Xiamen, Fujian, China.
authors.forename: Qing-Qing
authors.initials: QQ
authors.lastname: Liu
authors.affiliation: Laboratory Animal Center, Xiamen University, Xiamen, Fujian, China.
authors.forename: Yue-Hua
authors.initials: YH
authors.lastname: Zhang
authors.affiliation: Department of Basic Medical Sciences, Medical College, Xiamen University, Xiamen, Fujian, China.
authors.forename: Jing-Hua
authors.initials: JH
authors.lastname: Qiu
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism* / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D000818:Animals; D017318:Annexin A3 / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D002470:Cell Survival; D015536:Down-Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism* / Q000473:pathology*; D020935:MAP Kinase Signaling System; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D015398:Signal Transduction; D014945:Wound Healing
chemical_list: D017318:Annexin A3