title: Critical role of SP thymocyte motility in regulation of thymic output in neonatal Aire-/- mice.
abstract: Autoimmune regulator (Aire) is essential in the perinatal period to prevent the multiorgan autoimmunity. Here we show that Aire-regulated single positive thymocyte trafficking in neonatal period is critical for thymic egress. Reduced thymic emigration was found in Aire-/- mice during neonatal period, leading to enhanced homeostatic expansion of peripheral T cells as early as 2 weeks of age. In neonatal Aire-/- mice, thymic expression of CCR7 ligands were dramatically reduced, resulting in decreased thymocyte motility and thymocyte emigration. This reduction of thymic egress in Aire-/- mice was alleviated beyond 3 weeks of age by an early upregulation of S1P1 signaling. As the numbers and quality of thymic emigrants are essential for the establishment and maintenance of peripheral tolerance, the reduced thymic emigration during neonatal period may deteriorate autoimmunity caused by the emigration of autoreactive T cells.
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health. Peking University Health Science Center, Beijing, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Jin
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health. Peking University Health Science Center, Beijing, China.
authors.forename: Abudureyimujiang
authors.initials: A
authors.lastname: Aili
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health. Peking University Health Science Center, Beijing, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health. Peking University Health Science Center, Beijing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health. Peking University Health Science Center, Beijing, China.
authors.forename: Xiuyuan
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health. Peking University Health Science Center, Beijing, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Immunology, Key Laboratory of Medical Immunology, Ministry of Health. Peking University Health Science Center, Beijing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Ge
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers; D002465:Cell Movement / Q000235:genetics* / Q000276:immunology*; D006706:Homeostasis; D008213:Lymphocyte Activation; D018655:Lymphocyte Count; D051379:Mice; D018345:Mice, Knockout; D010641:Phenotype; D054398:Receptors, CCR4 / Q000378:metabolism; D054400:Receptors, CCR7 / Q000378:metabolism; D049349:Receptors, Lysosphingolipid / Q000378:metabolism; D060168:Thymocytes / Q000276:immunology* / Q000378:metabolism*; D013950:Thymus Gland / Q000502:physiology*; D014157:Transcription Factors / Q000172:deficiency / Q000235:genetics*; D000096927:AIRE Protein
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D054398:Receptors, CCR4; D054400:Receptors, CCR7; D049349:Receptors, Lysosphingolipid; D014157:Transcription Factors; D000096927:AIRE Protein
keywords: Aire deficiency; CCR7; Immune response; Immunity; Immunology and Microbiology Section; neonatal thymic emigration; thymocyte motility