title: Phenolic constituents from the root bark of Morus alba L. and their cardioprotective activity in vitro.
abstract: A flavanone C-glycoside, steppogenin-5'-C-β-D-glucopyranoside, six prenylated 2-arylbenzofuran derivatives, moracin O-3″-O-β-D-glucopyranoside, moracin O-3'-O-β-D-xylopyranoside, moracin P-2″-O-β-D-glucopyranoside, moracin P-3'-O-β-D-glucopyranoside, moracin P-3'-O-α-L-arabinopyranoside and moracin P-3'-O-[β-D-glucopyranosyl-(1 → 2)]-α-L-arabinopyranoside, two phenolic acids, 2,4-dihydroxy-5-(4-hydroxybenzyl) benzoic acid and 2,4-dihydroxy-5-(3,4-dihydroxybenzyl) benzoic acid, as well as three known compounds, moracinoside C, moracin O, and moracin P were isolated from the root bark of Morus alba L. Their structures were ascertained on the basis of spectroscopic evidence. The protective effects of the compounds against doxorubicin-induced cardiomyopathy in H9c2 cells was investigated in vitro. Of all of the isolated compounds, moracin P-3'-O-β-D-glucopyranoside, moracin O and moracin P had a strong protective influence against doxorubicin-induced cell death with EC50values of 9.5 ± 2.6, 4.5 ± 1.3, and 8.8 ± 2.4 μM, respectively.
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Xiao-Ke
authors.initials: XK
authors.lastname: Zheng
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China; School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
authors.forename: Yan-Gang
authors.initials: YG
authors.lastname: Cao
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Ke
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China; School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
authors.forename: Jian-Hong
authors.initials: JH
authors.lastname: Gong
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Heilongjiang University of Chinese Medicine, Harbin 150040, PR China.
authors.forename: Hai-Xue
authors.initials: HX
authors.lastname: Kuang
authors.affiliation: School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, PR China. Electronic address: fwsh@hactcm.edu.cn.
authors.forename: Wei-Sheng
authors.initials: WS
authors.lastname: Feng
mesh_terms: D001572:Benzofurans / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D002316:Cardiotonic Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006027:Glycosides / Q000032:analysis; D015394:Molecular Structure; D031625:Morus / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D024301:Plant Bark / Q000737:chemistry
chemical_list: D001572:Benzofurans; D002316:Cardiotonic Agents; D005419:Flavonoids; D006027:Glycosides; D010636:Phenols; C540981:moracin O; C540980:moracin P
keywords: Cardioprotective effect; Moraceae; Morus alba L.; Phenolic constituents