title: High titer heterologous rhamnolipid production.
abstract: Heterologous mono-rhamnolipid production by Pseudomonas putida KT2440 pSynPro8oT_rhlAB using glucose as the single carbon source was characterized in fed-batch bioreactor cultivations. For the described experiments, a defined mineral salt medium was used, and a two phase glucose feeding profile was applied, which yielded a final rhamnolipid concentration of 14.9 g/L. Applying the feeding profile, glucose stayed almost constant until 28 h of cultivation and decreased afterwards to limiting levels. Until the end of cultivation 253.0 ± 0.1 g glucose was added to the bioreactor of which a total of 252.0 ± 0.6 g glucose was metabolized. By modeling the fed-batch bioreactor cultivations the time courses of generated biomass, rhamnolipid and consumed glucose were described. The model was furthermore used to derive key process parameters from the collected data. The obtained values for the specific product formation rates (qRL) reached 18 mg/(g h) and yield coefficients (YRL/S) 10 mg/g respectively.
authors.affiliation: Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany.|Evonik Industries, Evonik Technology and Infrastructure GmbH, Rodenbacher Chaussee 4, 63457, Hanau-Wolfgang, Germany.
authors.forename: Janina
authors.initials: J
authors.lastname: Beuker
authors.affiliation: Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany.
authors.forename: Theresa
authors.initials: T
authors.lastname: Barth
authors.affiliation: Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany.
authors.forename: Anke
authors.initials: A
authors.lastname: Steier
authors.affiliation: Ulm Center for Peptide Pharmaceuticals (U-PEP), Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Wittgens
authors.affiliation: Ulm Center for Peptide Pharmaceuticals (U-PEP), Ulm University, Albert-Einstein-Allee 11, 89081, Ulm, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Rosenau
authors.affiliation: Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany. Marius.Henkel@uni-hohenheim.de.
authors.forename: Marius
authors.initials: M
authors.lastname: Henkel
authors.affiliation: Department of Bioprocess Engineering (150k), Institute of Food Science and Biotechnology, University of Hohenheim, Fruwirthstr. 12, 70599, Stuttgart, Germany.
authors.forename: Rudolf
authors.initials: R
authors.lastname: Hausmann
keywords: Biosurfactant; Fed batch cultivation; Heterologous rhamnolipid biosynthesis; Pseudomonas putida