title: Bioinformatics Analysis and Validation of the Expressed Sequences Tag in Human Colorectal Adenocarcinoma.
abstract: BACKGROUND
  This study was to investigate some new pathological genes in colorectal adenocarcinoma of human.
  
  
  METHODS
  Human colorectal adenocarcinoma tissues and normal colorectal tissues were taken and suppression subtractive hybridization (SSH) and cDNA microarray techniques were employed. From differentially expressed 86 expressed sequence tags (EST), 10 EST of the SSH were selected as seed sequence for bioinformatics analyses, semi-quantitative RT-PCR and PCR-sequencing. Each lane of semi-quantitative RT-PCR was analyzed by Q1 software.
  
  
  RESULTS
  Among these 10 EST, it has been found that ES274070, ES274071, ES274076 and ES274081 may play role in the onset of colorectal adenocarcinoma in human.
  
  
  CONCLUSIONS
  The ES274070, ES274071, ES274076 and ES274081 are related to the onset of human colorectal adenocarcinoma.
authors.affiliation: Department of Anatomy, Basic Medical and Legal Medical Institute of Sichuan university. Chengdu, Sichuan, China. 610041.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Anatomy, Basic Medical and Legal Medical Institute of Sichuan university. Chengdu, Sichuan, China. 610041.
authors.forename: Chun Le
authors.initials: CL
authors.lastname: Zhang
authors.affiliation: Department of Anatomy, Basic Medical and Legal Medical Institute of Sichuan university. Chengdu, Sichuan, China. 610041.
authors.forename: Yong Qiang
authors.initials: YQ
authors.lastname: Shen
authors.affiliation: Department of Anatomy, Basic Medical and Legal Medical Institute of Sichuan university. Chengdu, Sichuan, China. 610041.
authors.forename: Li Cheng
authors.initials: LC
authors.lastname: Wang
keywords: Bioinformatic analyses; Colorectal adenocarcinoma; Expressed sequence tag