title: Responses of primary cultured haemocytes derived from the marine gastropod Haliotis tuberculata to an industrial effluent exposure.
abstract: This study assessed the responses of primary cultured haemocytes from the marine gastropod Haliotis tuberculata exposed to the increasing concentrations of industrial effluent (0, 0.5, 1, 10, 15 and 20%) discharged into the Tunisian coastal area. Analyses showed the presence of metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), zinc (Zn), nickel (Ni) and lead (Pb) in the effluent. The effects of this mixture of pollutants on abalone haemocyte parameters were reflected by a significant decrease of cell viability, phagocytotic activity and reactive oxygen species (ROS) production as well as morphological and lysosomal membrane alterations. Thus, these results indicated that our primary culture system represents a suitable in vitro model for monitoring of anthropogenic contaminants in aquatic environments.
authors.affiliation: Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia. rladhar@yahoo.fr.
authors.forename: Rim
authors.initials: R
authors.lastname: Ladhar-Chaabouni
authors.affiliation: Normandie université, 14032, Caen, France.|UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Houel
authors.affiliation: Normandie université, 14032, Caen, France.|UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Serpentini
authors.affiliation: Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia.
authors.forename: Sahar
authors.initials: S
authors.lastname: Karray
authors.affiliation: Normandie université, 14032, Caen, France.|UMR BOREA (Biologie des Organismes et des Ecosystèmes Aquatiques), MNHN, UPMC, UCBN, CNRS-7208, IRD-207, IBFA, Université de Caen Normandie, Esplanade de la Paix, 14032, Caen Cedex 5, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Lebel
authors.affiliation: Marine Ecotoxicology UR 09-03, IPEIS, BP 805, 3018, Sfax, Tunisia.
authors.forename: Amel
authors.initials: A
authors.lastname: Hamza-Chaffai
keywords: Haemocytes; Haliotis tuberculata; Immune parameters; In vitro; Industrial effluent; Primary culture