title: Capsaicin partially mimics heat in mouse fibroblast cells in vitro.
abstract: Capsaicin activates transient receptor potential vanilloid 1 (TRPV1), a cation channel in the transient receptor potential family, resulting in the transient entry of Ca2+and Mg2+and a warm sensation. However, the effects of capsaicin on cells have not fully elucidated in fibroblasts. In this study, we investigated whether capsaicin could induce signal transduction in mouse fibroblast cells and compared the effect with that of heat-induced signal transduction. The activation of the mitogen-activated protein kinases (MAPKs) ERK and p38 MAPK, expression levels of heat shock protein 70 (HSP70) and HSP90, actin assembly, and cell proliferation were analyzed in NIH3T3 mouse fibroblast cells. A 15-min stimulation with capsaicin (∼100 μM) phosphorylated ERK and p38 MAPK and induced actin assembly. A 2-day stimulation with capsaicin increased the level of HSP70, but not HSP90, and the 2-day stimulation with capsaicin (∼100 μM) did not affect cell proliferation. A 15-min exposure to moderate heat (39.5 °C) phosphorylated both ERK and p38 MAPK and induced actin assembly to similar degrees as stimulation with capsaicin. A 2-day exposure to moderate heat increased the levels of both HSP70 and HSP90 and prevented cell proliferation. However, the 2-day stimulation with capsaicin (100 μM) failed to prevent heat shock-induced cell death. Thus, our results suggest that the effects of capsaicin on fibroblast cells partially differ from those of heat. Notably, the 2-day stimulation with capsaicin was not sufficient to develop heat tolerance in fibroblast cells.
authors.affiliation: Department of Physiology, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa, Ishikawa, 920-8640, Japan. ns@med.kanazawa-u.ac.jp.|Department of Environmental Physiology, School of Medicine, Shimane University, Izumo, 693-8501, Japan. ns@med.kanazawa-u.ac.jp.|Department of Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, 920-8640, Japan. ns@med.kanazawa-u.ac.jp.
authors.forename: Naotoshi
authors.identifier: http://orcid.org/0000-0003-1995-5002
authors.initials: N
authors.lastname: Sugimoto
authors.affiliation: Department of Environmental Physiology, School of Medicine, Shimane University, Izumo, 693-8501, Japan.|Department of Nutritional Physiology, Faculty of Pharmaceutical Sciences, Josai University, Sakado, 350-0295, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Katakura
authors.affiliation: Department of Environmental Physiology, School of Medicine, Shimane University, Izumo, 693-8501, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Matsuzaki
authors.affiliation: Department of Public Health Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, 920-8640, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: Department of Pediatrics, Graduate School of Medical Science, Kanazawa University, Kanazawa, 920-8640, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Yachie
authors.affiliation: Department of Environmental Physiology, School of Medicine, Shimane University, Izumo, 693-8501, Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Shido
mesh_terms: D000199:Actins / Q000378:metabolism; D000818:Animals; D002211:Capsaicin / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004789:Enzyme Activation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D018869:Heat-Shock Response; D051379:Mice; D041681:NIH 3T3 Cells; D010766:Phosphorylation; D015398:Signal Transduction / Q000187:drug effects; D013696:Temperature; D013997:Time Factors; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D000199:Actins; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases; D002211:Capsaicin
keywords: Capsaicin; Heat; TRP family; Temperature sense; Tolerance