title: Influence of static magnetic fields up to 700 mT and dihydrochalcones on the antioxidant response in fibroblasts.
abstract: The effects of a static magnetic field (SMF) and the dihydrochalcones phloretin and phloridzin on the redox homeostasis of fibroblasts were investigated. The aim of the present study was to determine the redox homeostasis of fibroblasts that were simultaneously exposed to a static magnetic field and the dihydrochalcones phloretin and phloridzin. The fibroblasts were cultured for 72 h in special magnetic test chambers at different moderate intensities (0.4, 0.55 and 0.7 T). In this report, the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), glutathione transferase (GST); the concentrations of malondialdehyde (MDA), adenosine triphosphate (ATP) and total antioxidant status were measured using commercially available kits. We did not observe any impairment in the redox balance in cells in fibroblasts that were only exposed to static magnetic fields of different intensities or In fibroblast cultured with dihydrochalcones and exposed to static magnetic field increase the SOD, GPx, GST activities and MDA concentration. Our investigations revealed that the activities of SOD, GPx, GST and the concentration of MDA that were determined for the fibroblasts that were cultured with dihydrochalcones were higher in the presence of a static magnetic field. Our results indicated that exposure to SMF (0.7 T) with dihydrochalcones induces oxidative stress in fibroblasts.
authors.affiliation: a Department of Food and Nutrition , School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia , Sosnowiec , Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Synowiec-Wojtarowicz
authors.affiliation: a Department of Food and Nutrition , School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia , Sosnowiec , Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Kimsa-Dudek
authors.affiliation: a Department of Food and Nutrition , School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia , Sosnowiec , Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Pawłowska-Góral
authors.affiliation: a Department of Food and Nutrition , School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia , Sosnowiec , Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Kurzeja
authors.affiliation: b Institute of Electrical Drives and Machines KOMEL , Katowice , Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Glinka
authors.affiliation: b Institute of Electrical Drives and Machines KOMEL , Katowice , Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Gawron
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D002478:Cells, Cultured / Q000187:drug effects / Q000528:radiation effects; D047188:Chalcones / Q000737:chemistry*; D005347:Fibroblasts / Q000187:drug effects* / Q000528:radiation effects*; D006706:Homeostasis / Q000187:drug effects* / Q000528:radiation effects*; D060526:Magnetic Fields / Q000009:adverse effects*; D051379:Mice; D018384:Oxidative Stress / Q000187:drug effects
chemical_list: D000975:Antioxidants; D047188:Chalcones; C015812:dihydrochalcone
keywords: Phloretin; phloridzin; redox homeostasis; static magnetic field