title: MtNF-YA1, A Central Transcriptional Regulator of Symbiotic Nodule Development, Is Also a Determinant ofMedicago truncatulaSusceptibility toward a Root Pathogen.
abstract: Plant NF-Y transcription factors control a wide array of biological functions enabling appropriate reproductive and developmental processes as well as adaptation to various abiotic and biotic environments. InMedicago truncatula,MtNF-YA1was previously identified as a key determinant for nodule development and establishment of rhizobial symbiosis. Here, we highlight a new role for this protein in compatibility toAphanomyces euteiches, a root pathogenic oomycete. TheMtnf-ya1-1mutant plants showed better survival rate, reduced symptoms, and increased development of their root apparatus as compared to their wild-type (WT) background A17.MtNF-YA-1was specifically up-regulated byA. euteichesin F83005.5, a highly susceptible natural accession ofM. truncatulawhile transcript level remained stable in A17, which is partially resistant. The role ofMtNF-YA1in F83005.5 susceptibility was further documented by reducingMtNF-YA1expression either by overexpression of the miR169q, a microRNA targetingMtNF-YA1, or by RNAi approaches leading to a strong enhancement in the resistance of this susceptible line. Comparative analysis of the transcriptome of WT andMtnf-ya1-1led to the identification of 1509 differentially expressed genes. Among those, almost 36 defense-related genes were constitutively expressed inMtnf-ya1-1, while 20 genes linked to hormonal pathways were repressed. In summary, we revealed an unexpected dual role for this symbiotic transcription factor as a key player in the compatibility mechanisms to a pathogen.
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS Castanet Tolosan, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rey
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, UMR441Castanet-Tolosan, France; Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, UMR2594Castanet-Tolosan, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Laporte
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS Castanet Tolosan, France.
authors.forename: Maxime
authors.initials: M
authors.lastname: Bonhomme
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, UMR441Castanet-Tolosan, France; Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, UMR2594Castanet-Tolosan, France.
authors.forename: Marie-Françoise
authors.initials: MF
authors.lastname: Jardinaud
authors.affiliation: POPS Transcriptomic Platform - Institute of Plant Sciences Paris-Saclay IPS2, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Sud, Université d'Évry Val-d'Essonne, Université Paris Diderot, Sorbonne Paris-Cite, Universite Paris-Saclay Orsay, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Huguet
authors.affiliation: POPS Transcriptomic Platform - Institute of Plant Sciences Paris-Saclay IPS2, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Université Paris-Sud, Université d'Évry Val-d'Essonne, Université Paris Diderot, Sorbonne Paris-Cite, Universite Paris-Saclay Orsay, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Balzergue
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS Castanet Tolosan, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Dumas
authors.affiliation: Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, UMR441Castanet-Tolosan, France; Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, UMR2594Castanet-Tolosan, France.
authors.forename: Andreas
authors.initials: A
authors.lastname: Niebel
authors.affiliation: Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, CNRS, UPS Castanet Tolosan, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Jacquet
keywords: Aphanomyces euteiches; Medicago truncatula; NF-Y transcription factor; Plant Roots; symbiosis and immunity