title: Brown adipogenic potential of brown adipocytes and peri-renal adipocytes from human embryo.
abstract: Both brown adipocytes (BAC) and beige cells hold therapeutic potential for the treatment of metabolic disorders. Unfortunately, the amount and activity of these cells are limited in adults. Although BAC marker expression has been shown in peri-renal adipose tissues in children and adults, functional assessment is lacking. Furthermore, it is entirely unknown whether adipose progenitors are present in human embryo and able to give rise to BAC in situ during evolution. Therefore, adipose tissues in the interscapular and peri-renal regions were dissected from human embryo and subcutaneous white adipose tissues (sWAT) were obtained from an adult. After subjected to differentiation in vitro, adipocyte progenitors were detected present in all these adipose tissues. When stimulated for adipogenesis, differentiated adipocytes in the intercapular and peri-renal regions showed similar features: (1) induced BAC and beige cell marker expression including UCP1 and PRDM16 and comparable mitochondrion copy number; (2) similar gene expression patterns by RNA-Seq analysis; and (3) similar maximal oxygen consumption rates examined by respirometry. Nevertheless, stimulation of adipocyte progenitors in sWAT induces neither BAC and beige cell marker expression nor any change of oxygen consumption. In conclusion, peri-renal adipocyte progenitors in human embryo hold browning potential for BAC production.
authors.affiliation: Beijing Key Laboratory of Diabetes Prevention and Research, Lu He hospital, Capital Medical University, Beijing 101149, China.|Department of Endocrinology, Lu He hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Nan-Nan
authors.initials: NN
authors.lastname: Wu
authors.affiliation: Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.|Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.
authors.forename: Chuan-Hai
authors.initials: CH
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Hyuek-Jong
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Beijing Key Laboratory of Diabetes Prevention and Research, Lu He hospital, Capital Medical University, Beijing 101149, China.|Department of Endocrinology, Lu He hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Diabetes Prevention and Research, Lu He hospital, Capital Medical University, Beijing 101149, China.|Department of Endocrinology, Lu He hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Key Laboratory of Diabetes Prevention and Research, Lu He hospital, Capital Medical University, Beijing 101149, China.|Department of Endocrinology, Lu He hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Ma
authors.affiliation: Department of Gynaecology and Obstetrics, Lu He hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ma
authors.affiliation: Beijing Key Laboratory of Diabetes Prevention and Research, Lu He hospital, Capital Medical University, Beijing 101149, China.|Department of Endocrinology, Lu He hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Beijing Key Laboratory of Diabetes Prevention and Research, Lu He hospital, Capital Medical University, Beijing 101149, China.|Department of Endocrinology, Lu He hospital, Capital Medical University, Beijing 101149, China.
authors.forename: Ying-Mei
authors.initials: YM
authors.lastname: Feng
mesh_terms: D052437:Adipocytes, Brown / Q000166:cytology / Q000378:metabolism*; D052438:Adipocytes, White / Q000166:cytology / Q000378:metabolism; D050156:Adipogenesis; D002478:Cells, Cultured; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology*; D006801:Humans; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism; D017423:Sequence Analysis, RNA; D013234:Stem Cells / Q000166:cytology / Q000378:metabolism; D020825:T-Box Domain Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9 / Q000235:genetics / Q000378:metabolism; D000071256:Uncoupling Protein 1 / Q000235:genetics / Q000378:metabolism
chemical_list: D004268:DNA-Binding Proteins; D008565:Membrane Proteins; C416371:PRDM16 protein, human; D020825:T-Box Domain Proteins; C108625:TBX1 protein, human; C578535:TMEM26 protein, human; D014157:Transcription Factors; D053261:Tumor Necrosis Factor Receptor Superfamily, Member 9; D000071256:Uncoupling Protein 1; D012313:RNA