title: Extraction of polysaccharides by autohydrolysis of spent coffee grounds and evaluation of their antioxidant activity.
abstract: The extraction of polysaccharides by autohydrolysis of spent coffee grounds (SCG) was studied. Experimental assays were performed using different temperatures (160-200°C), liquid/solid ratios (5-15ml water/g SCG) and extraction times (10-50min) in order to determine the conditions that maximize the extraction of polysaccharides with high antioxidant activity. Autohydrolysis was demonstrated to be an efficient technique to recover antioxidant polysaccharides from SCG. The best process conditions consisted in using 15ml water/g SCG, during 10min at 160°C. The polysaccharides obtained under these conditions were mainly in the form of galactomannans and arabinogalactans. They presented high antioxidant activity (assessed by four different methods), were thermostable in a large range of temperature, and had a typical carbohydrate pattern, being of interest for industrial applications, mainly in the food area.
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
authors.forename: Lina F
authors.initials: LF
authors.lastname: Ballesteros
authors.affiliation: Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057 Braga, Portugal.
authors.forename: José A
authors.initials: JA
authors.lastname: Teixeira
authors.affiliation: Department of Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands. Electronic address: S.I.Mussatto@tudelft.nl.
authors.forename: Solange I
authors.initials: SI
authors.lastname: Mussatto
mesh_terms: D000975:Antioxidants / Q000302:isolation & purification* / Q000494:pharmacology*; D003069:Coffee / Q000737:chemistry*; D006868:Hydrolysis; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology*; D013696:Temperature; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D003069:Coffee; D011134:Polysaccharides; D014867:Water
keywords: Antioxidant activity; Autohydrolysis; Polysaccharides; Spent coffee grounds; Structural properties