title: Protective effect of Anoectochilus roxburghii polysaccharide against CCl4-induced oxidative liver damage in mice.
abstract: This study investigated the isolation and characterization of Anoectochilus roxburghii polysaccharides (ARP), and further evaluated whether ARP possessed hepatoprotective activities against CCl4-induced oxidative liver damage in mice. ARP is comprised of glucose and galactose in a 1.9:1 molar ratio, and the molecular weight is 19.5kDa. ARP displayed significant scavenging effects against hydroxyl radical, superoxide anion radical, DPPH radical and a strong reducing power. In vivo experiment demonstrated ARP (150mg/kg) administrated to mice for 7days prior to carbon tetrachloride treatment, attenuated the elevated expression levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG) in serum and inhibited the formation of hepatic malondialdehyde (MDA). ARP pretreatment also increased antioxidant enzyme activities such as glutathione (GSH), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC) in the liver of CCl4-induced mice. Furthermore, hepatic histopathological changes induced by CCl4were significantly normalized by ARP pretreatment. These findings demonstrated that ARP possessed hepatoprotective effect against acute CCl4-induced liver damage by reducing lipid oxidation.
authors.affiliation: Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001,Guangdong, China; Department of Biology, Shantou University, Shantou 515063, Guangdong, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Department of Biology, Shantou University, Shantou 515063, Guangdong, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001,Guangdong, China; Department of Biology, Shantou University, Shantou 515063, Guangdong, China.
authors.forename: Lawei
authors.initials: L
authors.lastname: Yang
authors.affiliation: Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001,Guangdong, China; Department of Biology, Shantou University, Shantou 515063, Guangdong, China.
authors.forename: Qunwen
authors.initials: Q
authors.lastname: Pan
authors.affiliation: Environment protection of Shantou, Shantou 515000, Guangdong, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
authors.affiliation: Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001,Guangdong, China.
authors.forename: Yongfu
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Biology, Shantou University, Shantou 515063, Guangdong, China.
authors.forename: Meizhen
authors.initials: M
authors.lastname: Chen
authors.affiliation: Shanghai Institute of Materia Medica National Center for Drug Screening, Shanghai 201203, China; Department of Biology, Shantou University, Shantou 515063, Guangdong, China. Electronic address: cshch_009@163.com.
authors.forename: Shichao
authors.initials: S
authors.lastname: Cui
authors.affiliation: Department of Biology, Shantou University, Shantou 515063, Guangdong, China. Electronic address: jieyu_2009@126.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Yu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002251:Carbon Tetrachloride / Q000633:toxicity*; D056486:Chemical and Drug Induced Liver Injury / Q000097:blood / Q000378:metabolism* / Q000473:pathology / Q000517:prevention & control*; D005978:Glutathione / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D009005:Monosaccharides / Q000032:analysis; D029595:Orchidaceae / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects*; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000975:Antioxidants; D009005:Monosaccharides; D011134:Polysaccharides; D008315:Malondialdehyde; D002251:Carbon Tetrachloride; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Anoectochilus roxburghii; Hepatoprotective effects; Polysaccharide