title: Methods to Analyze EVs.
abstract: Research in the field of extracellular vesicles (EVs) is challenged by the small size of the nano-sized particles. Apart from the use of transmission and scanning electron microscopy, established technical platforms to visualize, quantify, and characterize nano-sized EVs were lacking. Recently, methodologies to characterize nano-sized EVs have been developed. This chapter aims to summarize physical principles of novel and conventional technologies to be used in the EV field and to discuss advantages and limitations.
authors.affiliation: Institute for Transfusion Medicine, University Hospital Essen, University Duisburg-Essen, Virchowstr. 179, 45147, Essen, Germany. bernd.giebel@uk-essen.de.
authors.forename: Bernd
authors.initials: B
authors.lastname: Giebel
authors.affiliation: Particle Metrix GmbH, Am Latumer See 13, 40668, Meerbusch, Germany.
authors.forename: Clemens
authors.initials: C
authors.lastname: Helmbrecht
mesh_terms: D000818:Animals; D001692:Biological Transport; D015415:Biomarkers; D055252:Cell-Derived Microparticles / Q000737:chemistry / Q000378:metabolism / Q000648:ultrastructure; D000067493:Dynamic Light Scattering; D000067128:Extracellular Vesicles / Q000737:chemistry / Q000378:metabolism* / Q000648:ultrastructure*; D005434:Flow Cytometry; D006801:Humans; D018625:Microscopy, Atomic Force; D010316:Particle Size
chemical_list: D015415:Biomarkers
keywords: Dynamic light scattering; Electron microscopy; Extracellular vesicles; Flow cytometry; Nanoparticle tracking analysis; Resistive pulse sensing