title: High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma.
abstract: B23, a multifunctional nucleolar protein, is overexpressed in numerous cancers and is associated with tumorigenesis. However, the clinical significance and potential role of B23 in bladder urothelial carcinoma remains to be elucidated. The present study observed that the mRNA and protein expression levels of B23 were increased in bladder cancer cells and tissues. The overexpression of B23 contributed to tumorigenesis and was associated with poor prognosis in bladder cancer patients. Silencing of B23 by short hairpin RNA inhibited tumor cell growth and colony formation. In addition, knockdown of B23 suppressed the phosphorylation of extracellular signal‑regulated kinase (ERK), resulting in the inactivation of the ERK signaling pathway. Therefore, the present study indicated that B23 promotes bladder cancer cell growth via activation of the ERK signaling pathway and is a novel potential biomarker for the diagnosis and prognosis of bladder cancer.
authors.affiliation: Department of Urology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Gangjun
authors.initials: G
authors.lastname: Yuan
authors.affiliation: Department of Urology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Bai
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Department of Urology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Yakun
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Urology, Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Qiu
mesh_terms: D000367:Age Factors; D000368:Aged; D002295:Carcinoma, Transitional Cell / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism*; D000090243:Nucleophosmin; D010766:Phosphorylation; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D012189:Retrospective Studies; D015398:Signal Transduction; D001743:Urinary Bladder / Q000378:metabolism; D001749:Urinary Bladder Neoplasms / Q000175:diagnosis / Q000378:metabolism / Q000473:pathology*
chemical_list: D009687:Nuclear Proteins; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D000090243:Nucleophosmin; D048049:Extracellular Signal-Regulated MAP Kinases