title: Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia.
abstract: We investigated the in vivo role of protein degradation during intermediate (ITM) and long-term memory (LTM) in Aplysia using an operant learning paradigm. The proteasome inhibitor MG-132 inhibited the induction and molecular consolidation of LTM with no effect on ITM. Remarkably, maintenance of steady-state protein levels through inhibition of protein synthesis using either anisomycin or rapamycin in conjunction with proteasome inhibition permitted the formation of robust 24 h LTM. Our studies suggest a primary role for proteasomal activity in facilitation of gene transcription for LTM and raise the possibility that synaptic mechanisms are sufficient to sustain 24 h memory.
authors.affiliation: Department of Biological Science, Program in Neuroscience, Florida State University, Tallahassee, Florida 32306-4295, USA lyons@bio.fsu.edu.
authors.forename: Lisa C
authors.initials: LC
authors.lastname: Lyons
authors.affiliation: Department of Biological Science, Program in Neuroscience, Florida State University, Tallahassee, Florida 32306-4295, USA.
authors.forename: Jacob S
authors.initials: JS
authors.lastname: Gardner
authors.affiliation: Department of Biological Science, Program in Neuroscience, Florida State University, Tallahassee, Florida 32306-4295, USA.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Gandour
authors.affiliation: Department of Biological Science, Program in Neuroscience, Florida State University, Tallahassee, Florida 32306-4295, USA.
authors.forename: Harini C
authors.initials: HC
authors.lastname: Krishnan
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D000841:Anisomycin / Q000494:pharmacology; D001048:Aplysia / Q000187:drug effects / Q000502:physiology*; D003216:Conditioning, Operant / Q000187:drug effects / Q000502:physiology*; D015853:Cysteine Proteinase Inhibitors / Q000494:pharmacology; D007976:Leupeptins / Q000494:pharmacology; D057567:Memory, Long-Term / Q000187:drug effects / Q000502:physiology*; D046988:Proteasome Endopeptidase Complex / Q000187:drug effects / Q000378:metabolism*; D013997:Time Factors
chemical_list: D015853:Cysteine Proteinase Inhibitors; D007976:Leupeptins; D000841:Anisomycin; D046988:Proteasome Endopeptidase Complex; C072553:benzyloxycarbonylleucyl-leucyl-leucine aldehyde