title: Sex differences in response to activity-based anorexia model in C57Bl/6 mice.
abstract: Anorexia nervosa is a severe eating disorder often associated with physical hyperactivity and is more frequently observed in female sex. Activity-Based Anorexia (ABA) model combines physical activity (PA) and reduced food intake and thus allows a better understanding of the mechanisms underlying anorexia nervosa. We aimed to assess sex differences in response to ABA model in C57Bl/6 mice. Twenty four male and 16 female C57BL/6 mice were studied. ABA mice were placed in individual cages with a continuously recorded activity wheel. ABA mice had a progressive limited food access from 6h/day (day 6) to 3h/day (day 9) until the end of the protocol (day 17). Body weight and food intake were daily measured. We studied physical activity during 24h, during the dark phase (D-PA) and the light phase (L-PA). We also evaluated the feeding anticipatory physical activity (A-PA), the physical activity during food intake period (FI-PA) and the post-prandial physical activity (PP-PA). We observed 16.7% of mortality in males (4 out of 24 mice) during ABA protocol while no female mice died (p=0.09). At day 17, food intake was significantly higher in females than in males (p<0.05) that was associated with a lower body weight loss than in females (p<0.05). Before limited food access, no gender differences in wheel running activity were observed. From day 9, A-PA significantly increased over time in males (p<0.05 vs females) while females exhibited higher FI-PA and PP-PA (p<0.05 vs males). Correlations between wheel running activities and, respectively, food intake and body weight loss showed gender differences, in particularly for L-PA and A-PA. Our results suggest a greater susceptibility of male mice to develop ABA, males and females exhibit different patterns of physical activity after limitation of food access. Underlying mechanisms should be further investigated.
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France; Rouen University Hospital, Nutrition Department, Rouen, France.
authors.forename: Najate
authors.initials: N
authors.lastname: Achamrah
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Nobis
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France.
authors.forename: Alexis
authors.initials: A
authors.lastname: Goichon
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Breton
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Legrand
authors.affiliation: Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France; Animal Behavior Platform SCAC, University of Rouen-Normandy, France.
authors.forename: Jean Luc
authors.initials: JL
authors.lastname: do Rego
authors.affiliation: Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France; Animal Behavior Platform SCAC, University of Rouen-Normandy, France.
authors.forename: Jean Claude
authors.initials: JC
authors.lastname: do Rego
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France; Rouen University Hospital, Nutrition Department, Rouen, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Déchelotte
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France.
authors.forename: Sergueï O
authors.initials: SO
authors.lastname: Fetissov
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France; Rouen University Hospital, Nutrition Department, Rouen, France.
authors.forename: Liliana
authors.initials: L
authors.lastname: Belmonte
authors.affiliation: Normandie Univ, INSERM unit 1073 "Nutrition, Inflammation and dysfunction of gut-brain axis", Rouen, France; Institute for Research and Innovation in Biomedicine (IRIB), University of Rouen-Normandy, France; Rouen University Hospital, Nutrition Department, Rouen, France. Electronic address: moise.coeffier@univ-rouen.fr.
authors.forename: Moïse
authors.initials: M
authors.lastname: Coëffier
mesh_terms: D000818:Animals; D000856:Anorexia Nervosa / Q000401:mortality / Q000503:physiopathology*; D001835:Body Weight; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D004435:Eating / Q000502:physiology; D005260:Female; D008297:Male; D008810:Mice, Inbred C57BL; D009043:Motor Activity / Q000502:physiology*; D017440:Photoperiod; D012420:Running / Q000502:physiology; D012727:Sex Characteristics