title: Graphitic Carbon Nitride Nanosheets-Based Ratiometric Fluorescent Probe for Highly Sensitive Detection of H2O2and Glucose.
abstract: Graphitic carbon nitride (g-C3N4) nanosheets, an emerging graphene-like carbon-based nanomaterial with high fluorescence and large specific surface areas, hold great potential for biosensor applications. Current g-C3N4nanosheets based fluorescent biosensors majorly rely on single fluorescent intensity reading through fluorescence quenching interactions between the nanosheets and metal ions. Here we report for the first time the development of a novel g-C3N4nanosheets-based ratiometric fluorescence sensing strategy for highly sensitive detection of H2O2and glucose. With o-phenylenediamine (OPD) oxidized by H2O2in the presence of horseradish peroxidase (HRP), the oxidization product can assemble on the g-C3N4nanosheets through hydrogen bonding and π-π stacking, which effectively quenches the fluorescence of g-C3N4while delivering a new emission peak. The ratiometric signal variations enable robust and sensitive detection of H2O2. On the basis of the glucose converting into H2O2through the catalysis of glucose oxidase, the g-C3N4-based ratiometric fluorescence sensing platform is also exploited for glucose assay. The developed strategy is demonstrated to give a detection limit of 50 nM for H2O2and 0.4 μM for glucose, at the same time, it has been successfully used for glucose levels detection in human serum. This strategy may provide a cost-efficient, robust, and high-throughput platform for detecting various species involving H2O2-generation reactions for biomedical applications.
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Jin-Wen
authors.initials: JW
authors.lastname: Liu
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Yu-Min
authors.initials: YM
authors.lastname: Wang
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Lu-Ying
authors.initials: LY
authors.lastname: Duan
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: Jiang
authors.affiliation: Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, China.
authors.forename: Ru-Qin
authors.initials: RQ
authors.lastname: Yu
mesh_terms: D005456:Fluorescent Dyes; D005947:Glucose; D006108:Graphite; D006801:Humans; D006861:Hydrogen Peroxide; D049329:Nanostructures; D009570:Nitriles
chemical_list: D005456:Fluorescent Dyes; D009570:Nitriles; C011206:cyanogen; D006108:Graphite; D006861:Hydrogen Peroxide; D005947:Glucose
keywords: H2O2; glucose; graphitic carbon nitride nanosheets; ratiometric