title: HPLC Identification of Copper (II)-Trans-Resveratrol Complexes in Ethanolic Aqueous Solution.
abstract: Reversed-phase high-performance liquid chromatography assay with the diode array detector was applied for detection of trans-resveratrol complexes with copper (II). Two complexes with copper to resveratrol ratio 3:2 and 1:1 were identified in ethanolic-aqueous solutions in neutral and acidic conditions. The matrix-assisted laser desorption ionization-time of flight mass spectrometry was used for evaluation of complexes in eluate liquid fraction. The structures of complexes were modeled by Titan, Spartan and HyperChem software. The findings obtained satisfactorily explain the chromatographic data and could provide useful an additional data about these forms in which is present trans-resveratrol in wine (free and/or forming complex with copper).
authors.affiliation: Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4A, 20-093Lublin, Poland.
authors.forename: J
authors.initials: J
authors.lastname: Flieger
authors.affiliation: Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4A, 20-093Lublin, Poland.
authors.forename: M
authors.initials: M
authors.lastname: Tatarczak-Michalewska
authors.affiliation: Department of Analytical Chemistry, Medical University of Lublin, Chodzki 4A, 20-093Lublin, Poland.
authors.forename: E
authors.initials: E
authors.lastname: Blicharska
authors.affiliation: Department of Inorganic Chemistry, Medical University of Lublin, Chodzki 4A, 20-093Lublin, Poland.
authors.forename: R
authors.initials: R
authors.lastname: Swieboda
authors.affiliation: Department of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences, Gleboka 30, 20-612Lublin, Poland.
authors.forename: T
authors.initials: T
authors.lastname: Banach
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003300:Copper / Q000737:chemistry*; D009942:Organometallic Compounds / Q000032:analysis* / Q000737:chemistry*; D000077185:Resveratrol; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013267:Stilbenes / Q000032:analysis* / Q000737:chemistry*; D014920:Wine / Q000032:analysis
chemical_list: D009942:Organometallic Compounds; D013267:Stilbenes; D003300:Copper; D000077185:Resveratrol