title: Aldehyde-functionalized dendritic mesoporous silica nanoparticles as potential nanocarriers for pH-responsive protein drug delivery.
abstract: We developed a potential pH-responsive protein drug delivery system based on aldehyde-functionalized dendritic mesoporous silica nanoparticles (DMSNs-CHO) as nanocarriers. The structure, protein drug loading and release behavior, in vitro cytotoxicity and cell uptake of the DMSNs-CHO nanoparticles were investigated. The results showed that the uniform DMSNs-CHO nanoparticles had an average particle size of 174±17nm and a mesopore size of 7.7nm. Using bovine serum albumin (BSA) as a model protein drug, BSA could be loaded into DMSNs-CHO nanoparticles owing to the formation of imine bonds between aldehyde groups and primary amines of BSA molecules. BSA loading capacity was about 136μg/mg, and BSA release from DMSNs-CHO nanocarriers was dependent on pH environment. Furthermore, the DMSNs-CHO nanoparticles had no cytotoxicity and could be efficiently taken up by cells. Therefore, the DMSNs-CHO nanoparticles would be promising as nanocarriers for pH-responsive delivery of protein drugs.
authors.affiliation: Hubei Key Laboratory of Processing and Application of Catalytic Materials, College of Chemical Engineering, Huanggang Normal University, Huanggang 438000, China.
authors.forename: Zhengfang
authors.initials: Z
authors.lastname: Tian
authors.affiliation: School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Hubei Key Laboratory of Processing and Application of Catalytic Materials, College of Chemical Engineering, Huanggang Normal University, Huanggang 438000, China; School of Materials Science and Engineering, University of Shanghai for Science and Technology, 516 Jungong Road, Shanghai 200093, China. Electronic address: zjf2412@163.com.
authors.forename: Yufang
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000447:Aldehydes / Q000737:chemistry; D000818:Animals; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006863:Hydrogen-Ion Concentration; D051379:Mice; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D016062:Porosity; D000067996:RAW 264.7 Cells; D012710:Serum Albumin, Bovine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D012822:Silicon Dioxide / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D000447:Aldehydes; D004337:Drug Carriers; D012710:Serum Albumin, Bovine; D012822:Silicon Dioxide
keywords: Aldehyde functionalization; Controlled release; Drug delivery; Mesoporous silica