title: Effects of Quercetin in a Rat Model of Hemorrhagic Traumatic Shock and Reperfusion.
abstract: BACKGROUND
  We hypothesized that treatment with quercetin could result in improved hemodynamics, lung inflammatory parameters and mortality in a rat model of hemorrhagic shock.
  
  
  METHODS
  Rats were anesthetized (80 mg/kg ketamine plus 8 mg/kg xylazine i.p.). The protocol included laparotomy for 15 min (trauma), hemorrhagic shock (blood withdrawal to reduce the mean arterial pressure to 35 mmHg) for 75 min and resuscitation by re-infusion of all the shed blood plus lactate Ringer for 90 min. Intravenous quercetin (50 mg/kg) or vehicle were administered during resuscitation.
  
  
  RESULTS
  There was a trend for increased survival 84.6% (11/13) in the treated group vs. the shock group 68.4% (13/19,p> 0.05 Kaplan-Meier). Quercetin fully prevented the development of lung edema. The activity of aSMase was increased in the shock group compared to the sham group and the quercetin prevented this effect. However, other inflammatory markers such as myeloperoxidase activity, interleukin-6 in plasma or bronchoalveolar fluid were similar in the sham and shock groups. We found no bacterial DNA in plasma in these animals.
  
  
  CONCLUSIONS
  Quercetin partially prevented the changes in blood pressure and lung injury in shock associated to hemorrhage and reperfusion.
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. vchamo26@hotmail.com.|Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. vchamo26@hotmail.com.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. vchamo26@hotmail.com.
authors.forename: Virginia
authors.initials: V
authors.lastname: Chamorro
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. pandolfi.rachele@gmail.com.|Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. pandolfi.rachele@gmail.com.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. pandolfi.rachele@gmail.com.
authors.forename: Rachele
authors.initials: R
authors.lastname: Pandolfi
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. lmorenog@med.ucm.es.|Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. lmorenog@med.ucm.es.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. lmorenog@med.ucm.es.
authors.forename: Laura
authors.initials: L
authors.lastname: Moreno
authors.affiliation: Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. biancabarreira@med.ucm.es.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. biancabarreira@med.ucm.es.
authors.forename: Bianca
authors.initials: B
authors.lastname: Barreira
authors.affiliation: Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. and.mrt@gmail.com.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. and.mrt@gmail.com.
authors.forename: Andrea
authors.initials: A
authors.lastname: Martínez-Ramas
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. danmorca@gmail.com.|Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. danmorca@gmail.com.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. danmorca@gmail.com.
authors.forename: Daniel
authors.initials: D
authors.lastname: Morales-Cano
authors.affiliation: Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. ruizcabe@farm.ucm.es.|Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Melchor Fernández Almagro 3, Madrid 28029, Spain. ruizcabe@farm.ucm.es.
authors.forename: Jesús
authors.initials: J
authors.lastname: Ruiz-Cabello
authors.affiliation: Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. joseangel.lorente@salud.madrid.org.|Servicio de Medicina Intensiva, Hospital Universitario de Getafe, Getafe, Madrid 28905, Spain. joseangel.lorente@salud.madrid.org.|Universidad Europea de Madrid, Madrid 28905, Spain. joseangel.lorente@salud.madrid.org.
authors.forename: José Angel
authors.initials: JA
authors.lastname: Lorente
authors.affiliation: Departamento de Farmacología, Facultad de Farmacia, Universidad de Granada, Granada 18071, Spain. jmduarte@ugr.es.|Centro de Investigaciones Biomédicas en Red de Enfermedades Cardiovasculares (Ciberec), Madrid 28029, Spain. jmduarte@ugr.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Duarte
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. acogolludo@med.ucm.es.|Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. acogolludo@med.ucm.es.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. acogolludo@med.ucm.es.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Cogolludo
authors.affiliation: Departamento de Medicina, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. jlasw@separ.es.|Servicio de Neumología, Instituto de Investigación Sanitaria San Carlos (IdISSC), Madrid 28040, Spain. jlasw@separ.es.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Alvarez-Sala
authors.affiliation: Departamento de Farmacología, Facultad de Medicina, Universidad Complutense de Madrid, Madrid 28040, Spain. fperez@med.ucm.es.|Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (Ciberes), Madrid 28029, Spain. fperez@med.ucm.es.|Instituto de Investigación Sanitaria Gregorio Marañón (IISGM), Madrid 28007, Spain. fperez@med.ucm.es.
authors.forename: Francisco
authors.initials: F
authors.lastname: Perez-Vizcaino
mesh_terms: D000818:Animals; D062186:Arterial Pressure / Q000187:drug effects; D015415:Biomarkers / Q000097:blood; D004487:Edema / Q000517:prevention & control; D006439:Hemodynamics; D007249:Inflammation / Q000150:complications / Q000188:drug therapy; D015850:Interleukin-6 / Q000737:chemistry; D007552:Isotonic Solutions / Q000627:therapeutic use; D008297:Male; D009195:Peroxidase / Q000737:chemistry; D011654:Pulmonary Edema / Q000150:complications / Q000188:drug therapy; D011794:Quercetin / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar; D015424:Reperfusion; D012151:Resuscitation; D000077325:Ringer's Lactate; D012771:Shock, Hemorrhagic / Q000150:complications / Q000188:drug therapy*; D012774:Shock, Traumatic / Q000150:complications / Q000188:drug therapy*
chemical_list: D015415:Biomarkers; D015850:Interleukin-6; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; D011794:Quercetin; D009195:Peroxidase
keywords: hemorrhage; lung edema; reperfusion; systemic arterial pressure; trauma