title: Amelioration of Gamma-hexachlorocyclohexane (Lindane) induced renal toxicity byCamellia sinensisin Wistar rats.
abstract: OBJECTIVE
  A study to assess the toxic effects of gamma-hexachlorocyclohexane (γ-HCH) (lindane) and ameliorative effects ofCamellia sinensison renal system has been carried out in male Wistar rats.
  
  
  METHODS
  Four groups of rats with 18 each were maintained under standard laboratory hygienic conditions and provided feed and waterad libitum. γ-HCH was gavaged at 20 mg/kg b.wt. using olive oil as vehicle to Groups II.C. sinensisat 100 mg/kg b.wt. was administered orally in distilled water to Group IV in addition to γ-HCH 20 mg/kg b.wt. up to 45 days to study ameliorative effects. Groups I and III were treated with distilled water andC. sinensis(100 mg/kg b.wt.), respectively. Six rats from each group were sacrificed at fortnight intervals. Serum was collected for creatinine estimation. The kidney tissues were collected in chilled phosphate buffer saline for antioxidant profile and in also 10% buffered formalin for histopathological studies.
  
  
  RESULTS
  γ-HCH treatment significantly increased serum creatinine and significantly reduced the renal antioxidative enzymes catalase, superoxide dismutase, and glutathione peroxidase. Grossly, severe congestion was noticed in the kidneys. Microscopically, kidney revealed glomerular congestion, atrophy, intertubular hemorrhages, degenerative changes in tubular epithelium with vacuolated cytoplasm, desquamation of epithelium and urinary cast formation. A significant reduction in serum creatinine levels, significant improvement in renal antioxidant enzyme activities and near to normal histological appearance of kidneys in Group IV indicated that the green tea ameliorated the effects of γ-HCH, on renal toxicity.
  
  
  CONCLUSIONS
  This study suggested thatC. sinensisextract combined with γ-HCH could enhance antioxidant/detoxification system which consequently reduced the oxidative stress thus potentially reducing γ-HCH toxicity and tissue damage.
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India.
authors.forename: W L N V Vara
authors.initials: WL
authors.lastname: Prasad
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India.
authors.forename: Ch
authors.initials: C
authors.lastname: Srilatha
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Proddatur, Andhra Pradesh, India.
authors.forename: N
authors.initials: N
authors.lastname: Sailaja
authors.affiliation: Department of Veterinary Anatomy, NTR College of Veterinary Science, Sri Venkateswara Veterinary University, Gannavaram, Andhra Pradesh, India.
authors.forename: N K B
authors.initials: NK
authors.lastname: Raju
authors.affiliation: Department of Veterinary Pathology, College of Veterinary Science, Sri Venkateswara Veterinary University, Tirupati, Andhra Pradesh, India.
authors.forename: N
authors.initials: N
authors.lastname: Jayasree
keywords: Camellia sinensis; gamma-hexachlorocyclohexane; kidney