title: Curdlan induces selective mast cell degranulation without concomitant release of LTC4,IL-6 or CCL2.
abstract: Mast cells are sentinel cells with a tissue-specific localization in the interface between the host and the external environment. Their quick and selective response upon encountering pathogens is part of the innate host response and typically initiates the following adaptive immune response. Among several pattern recognition receptors (PRRs) involved in the recognition of pathogens by mast cells, the C-type lectin receptor Dectin-1 has been associated with the recognition of fungi. Our previous studies have shown that mast cells are the predominant cell type expressing Dectin-1 in human skin, and they also recognize and respond to Malassezia sympodialis by producing cytokines connected to the innate host response and upregulating the expression of Dectin-1. In the present study, we investigated mast cell responses to Curdlan, a β-glucan that acts as an agonist for the fungi receptor Dectin-1, and found a unique response pattern with induced degranulation, but surprisingly without synthesis of Leukotriene C4, IL-6 or CCL2. Since mast cells are the predominant Dectin-1 expressing cell in the human skin, this study suggests that mast cell degranulation in response to fungi is an important part of the first line of defense against these pathogens.
authors.affiliation: Clincial Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden; Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Valéria C
authors.initials: VC
authors.lastname: Barbosa-Lorenzi
authors.affiliation: Clincial Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Simon
authors.initials: S
authors.lastname: Peyda
authors.affiliation: Department of Clinical Science and Education, Karolinska Institutet, and Sachs' Children and Youth Hospital, Södersjukhuset, Stockholm, Sweden.
authors.forename: Annika
authors.initials: A
authors.lastname: Scheynius
authors.affiliation: Clincial Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.
authors.forename: Gunnar
authors.initials: G
authors.lastname: Nilsson
authors.affiliation: Clincial Immunology and Allergy Unit, Department of Medicine Solna, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden. Electronic address: carolina_lunderius@yahoo.se.
authors.forename: Carolina
authors.initials: C
authors.lastname: Lunderius-Andersson
mesh_terms: D000818:Animals; D015550:Cell Degranulation / Q000276:immunology*; D018932:Chemokine CCL2 / Q000378:metabolism*; D005089:Exocytosis / Q000276:immunology; D015850:Interleukin-6 / Q000378:metabolism*; D017997:Leukotriene C4 / Q000378:metabolism*; D008407:Mast Cells / Q000276:immunology* / Q000378:metabolism*; D051379:Mice; D017455:Receptors, IgE / Q000378:metabolism; D047071:beta-Glucans / Q000276:immunology*
chemical_list: D018932:Chemokine CCL2; D015850:Interleukin-6; D017455:Receptors, IgE; D047071:beta-Glucans; D017997:Leukotriene C4; C038459:curdlan
keywords: Curdlan; Dectin-1; Degranulation; Histamine; Mast cells