title: In Vivo Evaluation and Imaging of a Bilayered Self-Assembled Skin Substitute Using a Decellularized Dermal Matrix Grafted on Mice.
abstract: As time to final coverage is the essence for better survival outcome in severely burned patients, we have continuously strived to reduce the duration for the preparation of our bilayered self-assembled skin substitutes (SASS). These SASS produced in vitro by the self-assembly approach have a structure and functionality very similar to native skin. Recently, we have shown that a decellularized dermal matrix preproduced by the self-assembly approach could be used as a template to further obtain self-assembled skin substitute using a decellularized dermal template (SASS-DM) in vitro. Thus, the production period with patient cells was then reduced to about 1 month. Herein, preclinical animal experiments have been performed to confirm the integration and evolution of such a graft and compare the maturation of SASS and SASS-DM in vivo. Both tissues, reconstructed from adult or newborn cells, were grafted on athymic mice. Green fluorescent protein-transfected keratinocytes were also used to follow grafted tissues weekly for 6 weeks using an in vivo imaging system (IVIS). Cell architecture and differentiation were studied with histological and immunofluorescence analyses at each time point. Graft integration, macroscopic evolution, histological analyses, and expression of skin differentiation markers were similar between both skin substitutes reconstructed from either newborn or adult cells, and IVIS observations confirmed the efficient engraftment of SASS-DM. In conclusion, our in vivo graft experiments on a mouse model demonstrated that the SASS-DM had equivalent macroscopic, histological, and differentiation evolution over a 6-week period, when compared with the SASS. The tissue-engineered SASS-DM could improve clinical availability and advantageously shorten the time necessary for the definitive wound coverage of severely burned patients.
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .|3 Department of Plastic Surgery, Faculty of Medicine, University of Montreal , Quebec, Canada .
authors.forename: Chanel
authors.initials: C
authors.lastname: Beaudoin Cloutier
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .
authors.forename: Benjamin
authors.initials: B
authors.lastname: Goyer
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .
authors.forename: Cindy
authors.initials: C
authors.lastname: Perron
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .
authors.forename: Rina
authors.initials: R
authors.lastname: Guignard
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .
authors.forename: Danielle
authors.initials: D
authors.lastname: Larouche
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .
authors.forename: Véronique J
authors.initials: VJ
authors.lastname: Moulin
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .
authors.forename: Lucie
authors.initials: L
authors.lastname: Germain
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|4 Centre Québécois sur les Matériaux Fonctionnels (CQMF) , Quebec, Canada .
authors.forename: Robert
authors.initials: R
authors.lastname: Gauvin
authors.affiliation: 1 Centre de recherche en organogénèse expérimentale de l'Université Laval/LOEX and Département de chirurgie, Faculté de Médecine, Université Laval , Quebec, Canada .|2 Centre de recherche du CHU de Québec-Université Laval , Axe médecine régénératrice, Quebec, Canada .
authors.forename: François A
authors.initials: FA
authors.lastname: Auger
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D005347:Fibroblasts / Q000166:cytology; D049452:Green Fluorescent Proteins; D006801:Humans; D015603:Keratinocytes / Q000166:cytology; D008297:Male; D051379:Mice; D008819:Mice, Nude; D017768:Skin, Artificial; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D049452:Green Fluorescent Proteins
keywords: autologous; culture techniques; organoid; regenerative medicine; skin equivalent; tissue culture