title: Development of double chain phosphatidylcholine functionalized polymeric monoliths for immobilized artificial membrane chromatography.
abstract: This study described a simple synthetic methodology for preparing biomembrane mimicking monolithic column. The suggested approach not only simplifies the preparation procedure but also improves the stability of double chain phosphatidylcholine (PC) functionalized monolithic column. The physicochemical properties of the optimized monolithic column were characterized by scanning electron microscopy, energy-dispersive X-ray spectrometry, and nano-LC. Satisfactory column permeability, efficiency, stability and reproducibility were obtained on this double chain PC functionalized monolithic column. It is worth noting that the resulting polymeric monolith exhibits great potential as a useful alternative of commercial immobilized artificial membrane (IAM) columns for in vitro predication of drug-membrane interactions. Furthermore, the comparative study of both double chain and single chain PC functionalized monoliths indicates that the presence or absence of glycerol backbone and the number of acyl chains are not decisive for the predictive ability of IAM monoliths on drug-membrane interactions. This novel PC functionalized monolithic column also exhibited good selectivity for a protein mixture and a set of pharmaceutical compounds.
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Qiqin
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China; School of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China; School of Public Health, Guangdong Pharmaceutical University, Guangzhou 510006, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Peng
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Chen
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Peijie
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China.
authors.forename: Yuqiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: haibo.zhou@jnu.edu.cn.
authors.forename: Haibo
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Institute of Pharmaceutical Analysis, College of Pharmacy, Jinan University, Guangzhou 510632, China. Electronic address: jzjjackson@hotmail.com.
authors.forename: Zhengjin
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D002853:Chromatography, Liquid; D008567:Membranes, Artificial; D008855:Microscopy, Electron, Scanning; D004364:Pharmaceutical Preparations / Q000032:analysis / Q000302:isolation & purification; D010713:Phosphatidylcholines / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D013052:Spectrometry, X-Ray Emission; D013056:Spectrophotometry, Ultraviolet
chemical_list: D008567:Membranes, Artificial; D004364:Pharmaceutical Preparations; D010713:Phosphatidylcholines; D011108:Polymers
keywords: Double chain phosphatidylcholine; Drug-membrane interactions; Immobilized artificial membrane chromatography; Nano-LC; Polymeric monolith