title: Long-term influence of cyanobacterial bloom on the immune system of Litopenaeus vannamei.
abstract: Cyanobacteria are ubiquitously distributed in water on the Earth. It has long been known that the cyanobacterial bloom in aquaculture ponds can cause acute and massive deaths of shrimp. However, the long-term and chronic effects of the cyanobacterial bloom on shrimp are still poorly understood. In this study, the immune state of white pacific shrimp, Litopenaeus vannamei, surviving a naturally occurring cyanobacterial bloom was investigated and tracked for 70 d. Compared with the control, the growth of shrimp suffering high concentrations of cyanobacteria was obviously postponed. In these shrimp, the activities of the NF-κB, JAK/STAT and P38 MAPK immune signaling pathways and the expression of many antimicrobial peptide genes were down-regulated, whereas the expression of C-type lectins was significantly up-regulated. Although the mRNA level of lysozyme was reduced, the expression of the invertebrate-type lysozyme gene was increased. Furthermore, the concentration of hemocytes in hemolymph was greatly decreased, but the phagocytic activity of hemocytes was increased. These suggested that the cyanobacterial bloom has significant and complex influences on the immune system of shrimp, and in turn, alteration of the immune state could be a factor by which few shrimp can survive the cyanobacterial bloom. Thus, the current study could help further understand the interactions between the aquaculture water environment and the immune system of shrimp.
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Jiefeng
authors.initials: J
authors.lastname: Gao
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, PR China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zuo
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Linwei
authors.initials: L
authors.lastname: Yang
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Jian-Hui
authors.initials: JH
authors.lastname: He
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Shengwen
authors.initials: S
authors.lastname: Niu
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China.
authors.forename: Shaoping
authors.initials: S
authors.lastname: Weng
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, PR China. Electronic address: lsshjg@mail.sysu.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: He
authors.affiliation: MOE Key Laboratory of Aquatic Product Safety / State Key Laboratory for Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, PR China; Institute of Aquatic Economic Animals and Guangdong Provice Key Laboratory for Aquatic Economic Animals, Sun Yat-sen University, Guangzhou, PR China; Guangdong Provincial Key Laboratory of Marine Resources and Coastal Engineering, School of Marine Sciences, Sun Yat-sen University, Guangzhou, PR China; South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou, PR China. Electronic address: xuxpeng@mail.sysu.edu.cn.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Xu
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics* / Q000378:metabolism; D000458:Cyanobacteria / Q000502:physiology*; D005068:Eutrophication; D005786:Gene Expression Regulation; D006434:Hemocytes / Q000378:metabolism / Q000382:microbiology; D007113:Immunity, Innate; D037181:Lectins, C-Type / Q000235:genetics / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics / Q000276:immunology* / Q000382:microbiology; D010587:Phagocytosis; D015398:Signal Transduction
chemical_list: D060829:Arthropod Proteins; D037181:Lectins, C-Type
keywords: Antimicrobial peptide; C-type lectin; Cyanobacterial bloom; Immune signaling pathway; Invertebratetype lysozyme; Litopenaeus vannamei; Lysozyme; Phagocytic activity