title: Novel moisture-preserving derivatives of hyaluronan resistant to hyaluronidase and protective to UV light.
abstract: Current studies have revealed the excellent moisture absorption-retention capacity of hyaluronan (HA); however, HA is easily degraded by hyaluronidase on the surface of skin. So, it is very necessary to develop an alternative derivative with low cytotoxicity and resistance to hyaluronidase. Herein, a HA decorated with photocaged groups was synthesized. The moisture absorption-retention capacity and hyaluronidase resistance of photocaged HA (HA-DMNB) and products of HA-DMNB irradiated by ultraviolet for different time (IHA-DMNB), were investigated. Results show that HA-DMNB is more resistant to hyaluronidase than HA, and HA-DMNB could release free carboxyl groups of HA upon ultraviolet to bond with H2O. More importantly, HA-DMNB was protective against UV light. In addition, HA-DMNB and IHA-DMNB were observed to be nontoxic to HaCat cells. This study indicates that HA-DMNB may be effectively used as a moisture-preserving reagent.
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Sun
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Niu
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Dongdong
authors.initials: D
authors.lastname: Wang
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Mu
authors.affiliation: Endoscopy Center and Endoscopy Research Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China. Electronic address: ma.lili@zs-hospital.sh.cn.
authors.forename: Lili
authors.initials: L
authors.lastname: Ma
authors.affiliation: Shaanxi Key Laboratory of Natural Products & Chemical Biology, College of Science, Northwest A&F University, Yangling 712100, Shaanxi, China. Electronic address: jduan@nwsuaf.edu.cn.
authors.forename: Jinyou
authors.initials: J
authors.lastname: Duan
mesh_terms: D002460:Cell Line; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000528:radiation effects*; D006821:Hyaluronoglucosaminidase / Q000378:metabolism*; D007202:Indicators and Reagents; D012867:Skin; D014466:Ultraviolet Rays
chemical_list: D007202:Indicators and Reagents; D006820:Hyaluronic Acid; D006821:Hyaluronoglucosaminidase
keywords: Hyaluronan; Moisture-preserving property; Photocaging