title: Fidgetin regulates cultured astrocyte migration by severing tyrosinated microtubules at the leading edge.
abstract: Microtubule (MT) organization is essential for many cellular events, including mitosis, migration, and cell polarity. Fidgetin (Fign), an ATP-dependent, MT-severing protein, contributes to the regulation of MT configuration by cutting and trimming MT polymers. Functions of Fign have been indicated in neurite outgrowth, mitosis, meiosis, and cellular migration. Here we focus on migration of astrocytes. We find that Fign plays an essential role in cultured astrocyte migration by preferentially targeting MTs (or regions of MTs) that are rich in tyrosinated tubulin, a marker for especially dynamic MTs or especially dynamic regions of MTs. Inhibition of cellular migration induced by Fign knockdown can be rescued with concomitant knockdown of kinesin-12, a motor protein best known for its role in mitosis. We propose a novel working model for MT reconfiguration underlying cellular migration elicited by the functional cooperation of two distinct MT-related proteins.
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Zunlu
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Feng
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Weijuan
authors.initials: W
authors.lastname: Bo
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Wu
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Zhangji
authors.initials: Z
authors.lastname: Dong
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China liumei@ntu.edu.cn liang.qiang@drexelmed.edu.|Department of Neurobiology and Anatomy, Drexel University College of Medicine, Philadelphia, PA 19129.
authors.forename: Liang
authors.initials: L
authors.lastname: Qiang
authors.affiliation: Key Laboratory of Neuroregeneration, Jiangsu, and Ministry of Education, Co-innovation Center of Neuroregeneration, Nantong University, Nantong, Jiangsu 226001, China liumei@ntu.edu.cn liang.qiang@drexelmed.edu.
authors.forename: Mei
authors.initials: M
authors.lastname: Liu
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000378:metabolism* / Q000502:physiology; D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002465:Cell Movement / Q000502:physiology*; D002478:Cells, Cultured; D016547:Kinesins / Q000378:metabolism; D008869:Microtubule-Associated Proteins; D008870:Microtubules / Q000378:metabolism*; D055495:Neurogenesis; D009474:Neurons / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism* / Q000502:physiology; D051381:Rats; D014404:Tubulin / Q000378:metabolism; D014443:Tyrosine
chemical_list: D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D014404:Tubulin; D014443:Tyrosine; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; C498542:Fign protein, mouse; D016547:Kinesins