title: Biochemical Characterization of Echinococcus multilocularis Antigen B3 Reveals Insight into Adaptation and Maintenance of Parasitic Homeostasis at the Host-Parasite Interface.
abstract: Alveolar echinococcosis (AE) caused by Echinococcus multilocularis metacestode is frequently associated with deleterious zoonotic helminthiasis. The growth patterns and morphological features of AE, such as invasion of the liver parenchyme and multiplication into multivesiculated masses, are similar to those of malignant tumors. AE has been increasingly detected in several regions of Europe, North America, Central Asia, and northwestern China. An isoform of E. multilocularis antigen B3 (EmAgB3) shows a specific immunoreactivity against patient sera of active-stage AE, suggesting that EmAgB3 might play important roles during adaptation of the parasite to hosts. However, expression patterns and biochemical properties of EmAgB3 remained elusive. The protein profile and nature of component proteins of E. multilocularis hydatid fluid (EmHF) have never been addressed. In this study, we conducted proteome analysis of EmHF of AE cysts harvested from immunocompetent mice. We observed the molecular and biochemical properties of EmAgB3, including differential transcription patterns of paralogous genes, macromolecular protein status by self-assembly, distinct oligomeric states according to individual anatomical compartments of the worm, and hydrophobic ligand-binding protein activity. We also demonstrated tissue expression patterns of EmAgB3 transcript and protein. EmAgB3 might participate in immune response and recruitment of essential host lipids at the host-parasite interface. Our results might contribute to an in depth understanding of the biophysical and biological features of EmAgB3, thus providing insights into the design of novel targets to control AE.
authors.affiliation: Department of Molecular Parasitology, Sungkyunkwan University School of Medicine , Suwon 16419, Korea.
authors.forename: Chun-Seob
authors.initials: CS
authors.lastname: Ahn
authors.affiliation: Department of Molecular Parasitology, Sungkyunkwan University School of Medicine , Suwon 16419, Korea.
authors.forename: Jeong-Geun
authors.initials: JG
authors.lastname: Kim
authors.affiliation: Qinghai Province Institute for Endemic Diseases Prevention and Control , Xining 811602, China.
authors.forename: Xiumin
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Microbiology, Gachon University Graduate School of Medicine , Incheon 21936, Korea.
authors.forename: Young-An
authors.initials: YA
authors.lastname: Bae
authors.affiliation: Department of Biochemistry, Gachon University Graduate School of Medicine , Incheon 21936, Korea.
authors.forename: Woo-Jae
authors.initials: WJ
authors.lastname: Park
authors.affiliation: Department of Molecular Biology and Biochemistry, School of Medicine, Kyung Hee University , Seoul 02447, Korea.
authors.forename: Insug
authors.initials: I
authors.lastname: Kang
authors.affiliation: Qinghai Province Institute for Endemic Diseases Prevention and Control , Xining 811602, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Molecular Parasitology, Sungkyunkwan University School of Medicine , Suwon 16419, Korea.
authors.forename: Yoon
authors.identifier: 0000-0003-0905-4606
authors.initials: Y
authors.lastname: Kong
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics / Q000276:immunology; D000818:Animals; D003001:Cloning, Molecular; D004195:Disease Models, Animal; D004443:Echinococcosis; D004444:Echinococcosis, Hepatic / Q000235:genetics / Q000276:immunology* / Q000469:parasitology / Q000473:pathology; D048210:Echinococcus multilocularis / Q000254:growth & development / Q000276:immunology* / Q000472:pathogenicity; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D005786:Gene Expression Regulation; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D006706:Homeostasis / Q000235:genetics / Q000276:immunology; D006790:Host-Parasite Interactions; D006801:Humans; D008074:Lipoproteins / Q000235:genetics / Q000276:immunology*; D051379:Mice; D019301:Oleic Acid / Q000378:metabolism; D020543:Proteome / Q000235:genetics / Q000276:immunology*; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015801:Helminth Proteins; D008074:Lipoproteins; D020543:Proteome; D011994:Recombinant Proteins; C089273:antigen B, Echinococcus; D019301:Oleic Acid
keywords: Echinococcus multilocularis; alveolar echinococcosis; antigen B3; expressional regulation; hydatid fluid; hydrophobic ligand-binding protein; multimeric states; proteome