title: A recyclable protein resource derived from cauliflower by-products: Potential biological activities of protein hydrolysates.
abstract: Cauliflower by-products (CBP) are rich in leaf protein. Every year tons of CBP will lead to environmental pollution. Therefore, this study was conducted to extract leaf protein from CBP and investigate its biological activities. Our results showed that the optimal extraction parameters were: a liquid to solid ratio of 4mL/g, a pH of 11, an ultrasonic extraction lasting 15min, and at an applied power of 175W. Under these optimized conditions, 12.066g of soluble leaf protein (SLP) was obtained from 1000g of CBP and its extraction yield was 53.07%. The obtained SLP was further hydrolysed by Alcalase and the SLP hydrolysate (SLPH) showed a potent angiotensin I-converting enzyme (ACE) inhibitory activity with an IC50value of 138.545μg/mL in vitro. In addition, SLPH promoted the glucose consumption and enhanced the glycogen content in HepG2 cells. Overall, our results suggested that CBP may be recycled for designing future functional foods.
authors.affiliation: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China. Electronic address: xubajing@zju.edu.cn.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China.
authors.forename: Yuting
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Bao
authors.affiliation: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Zheng
authors.affiliation: Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China. Electronic address: zjuchenwei@zju.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Agricultural Science and Technology Research Extension Centre, Lanzhou 730010, China. Electronic address: yangxu93@zju.edu.cn.
authors.forename: Jianxu
authors.initials: J
authors.lastname: Wang
mesh_terms: D001937:Brassica / Q000378:metabolism*; D006868:Hydrolysis; D007703:Peptidyl-Dipeptidase A / Q000378:metabolism; D018515:Plant Leaves / Q000378:metabolism; D010940:Plant Proteins / Q000378:metabolism*; D011492:Protein Hydrolysates / Q000378:metabolism*; D013381:Subtilisins / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011492:Protein Hydrolysates; D007703:Peptidyl-Dipeptidase A; D013381:Subtilisins
keywords: ACE inhibitory activity; Cauliflower by-products; Glucose consumption; Glycogen content; Leaf protein; Ultrasonic-assisted extraction