title: Temperature-regulated expression of type VI secretion systems in fish pathogen Pseudomonas plecoglossicida revealed by comparative secretome analysis.
abstract: Pseudomonas plecoglossicida is a facultative fish pathogen. Recent studies showed that P. plecoglossicida infection in fish was associated with temperature. The aim of this study was to compare the secretomes of P. plecoglossicida cultured in vitro at representative temperatures for pathogenic (20°C) and less pathogenic (30°C) phenotypes. Thirteen proteins in the culture supernatants of P. plecoglossicida showed significant difference in abundance at 20 vs. 30°C. Four proteins were strongly increased at 20°C, including two hemolysin co-regulated proteins (Hcp) that are part of the bacterial type VI secretion system (T6SS), flagellin and an unknown protein. Immunoblot analysis verified the induced secretion of Hcps at 20°C. Furthermore, the upregulation of Hcps at 20°C was confirmed at transcriptional level by RT-qPCR analysis, which also demonstrated the induction of expression of other T6SS-related genes at 20°C. Taken together, we demonstrate the presence of two functionally active T6SS proteins in fish pathogenic P. plecoglossicida strains, as evidenced by the secretion of the T6SS substrate Hcp, the production of which were found to be controlled by temperature. Our findings also support efforts to develop vaccines targeting secreted virulence factors as prophylactic strategies for diseases in fish caused by P. plecoglossicida.
authors.affiliation: School of Marine Science, Ningbo University, 818 Feng-Hua Road, Ningbo, Zhejiang 315211, China taozhen@nbu.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Tao
authors.affiliation: School of Marine Science, Ningbo University, 818 Feng-Hua Road, Ningbo, Zhejiang 315211, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhou
authors.affiliation: School of Marine Science, Ningbo University, 818 Feng-Hua Road, Ningbo, Zhejiang 315211, China.
authors.forename: Suming
authors.initials: S
authors.lastname: Zhou
authors.affiliation: School of Marine Science, Ningbo University, 818 Feng-Hua Road, Ningbo, Zhejiang 315211, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Wang
mesh_terms: D000818:Animals; D005393:Fish Diseases / Q000382:microbiology*; D005408:Flagellin / Q000096:biosynthesis*; D015964:Gene Expression Regulation, Bacterial; D006460:Hemolysin Proteins / Q000096:biosynthesis*; D010473:Perciformes / Q000382:microbiology*; D011549:Pseudomonas / Q000254:growth & development / Q000378:metabolism* / Q000472:pathogenicity; D013696:Temperature; D000069376:Type VI Secretion Systems / Q000378:metabolism*; D037521:Virulence Factors / Q000096:biosynthesis
chemical_list: D006460:Hemolysin Proteins; D000069376:Type VI Secretion Systems; D037521:Virulence Factors; D005408:Flagellin
keywords: Pseudomonas plecoglossicida; T6SS; extracellular proteins; fish pathogen; secretome; temperature