title: Testosterone Deficiency Induces Changes of the Transcriptomes of Visceral Adipose Tissue in Miniature Pigs Fed a High-Fat and High-Cholesterol Diet.
abstract: Testosterone deficiency causes fat deposition, particularly in visceral fat, and its replacement might reverse fat accumulation, however, the underlying mechanisms of such processes under diet-induced adiposity are largely unknown. To gain insights into the genome-wide role of androgen on visceral adipose tissue (VAT), RNA-Seq was used to investigate testosterone deficiency induced changes of VAT in miniature pigs fed a high-fat and high-cholesterol (HFC) diet among intact male pigs (IM), castrated male pigs (CM), and castrated male pigs with testosterone replacement (CMT) treatments. The results showed that testosterone deficiency significantly increased VAT deposition and serum leptin concentrations. Moreover, a total of 1732 differentially expressed genes (DEGs) were identified between any two groups. Compared with gene expression profiles in IM and CMT pigs, upregulated genes in CM pigs, i.e.,LOC100520753(CD68),LCN2,EMR1,S100A9,NCF1(p47phox), andLEP, were mainly involved in inflammatory response, oxidation-reduction process, and lipid metabolic process, while downregulated genes in CM pigs, i.e.,ABHD5,SPP1, andGAS6, were focused on cell differentiation and cell adhesion. Taken together, our study demonstrates that testosterone deficiency alters the expression of numerous genes involved in key biological processes of VAT accumulation under HFC diet and provides a novel genome-wide view on the role of androgen on VAT deposition under HFC diet, thus improving our understanding of the molecular mechanisms involved in VAT changes induced by testosterone deficiency.
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. lifanzhang@njau.edu.cn.
authors.forename: Lifan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China. cyq810@hotmail.com.
authors.forename: Yueqin
authors.initials: Y
authors.lastname: Cai
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. sjwei@njau.edu.cn.
authors.forename: Shengjuan
authors.initials: S
authors.lastname: Wei
authors.affiliation: Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China. lyun1983@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Ling
authors.affiliation: Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China. tozhuliang@126.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China. lidongfeng@njau.edu.cn.
authors.forename: Dongfeng
authors.initials: D
authors.lastname: Li
authors.affiliation: Laboratory Animal Research Center, Zhejiang Chinese Medical University, Hangzhou 310053, China. zwcai@zcmu.edu.cn.
authors.forename: Zhaowei
authors.initials: Z
authors.lastname: Cai
mesh_terms: D050154:Adiposity / Q000235:genetics*; D000818:Animals; D002369:Castration; D002448:Cell Adhesion / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002784:Cholesterol / Q000378:metabolism*; D059305:Diet, High-Fat; D020869:Gene Expression Profiling; D006937:Hypercholesterolemia / Q000378:metabolism*; D007249:Inflammation / Q000235:genetics; D050152:Intra-Abdominal Fat / Q000166:cytology*; D020738:Leptin / Q000097:blood*; D008297:Male; D009765:Obesity / Q000378:metabolism; D010084:Oxidation-Reduction; D013552:Swine; D013556:Swine, Miniature; D013739:Testosterone / Q000097:blood / Q000172:deficiency* / Q000494:pharmacology*; D059467:Transcriptome / Q000235:genetics
chemical_list: D020738:Leptin; D013739:Testosterone; D002784:Cholesterol
keywords: RNA-Seq; inflammatory response; miniature pigs; testosterone deficiency; visceral adipose tissue