title: Manganese-enhanced MRI (ME MRI) in evaluation of the auditory pathway in an experimental rat model.
abstract: This study aimed to explore the optimal dose and manner of administration for visualization of the auditory pathway on manganese-enhanced MRI (ME MRI). Twenty-four healthy male Sprague-Dawley rats were randomly divided into three experimental groups (n = 8 for Groups A, B and C). The rats in Groups A, B and C were subjected to MnCl2injection through the tympanum, inner ear endolymph and perilymph, respectively (0.2 M for four rats and 0.4 M for the others in each group) and observed at 1, 2, 3, 4, 7 and 10 days after the operation with 3.0 T MRI. The signal intensity (SI) and dynamic changes of the auditory pathways at various times, and at two doses through three injection routes, were compared by statistical analysis. Administration of MnCl2through the perilymph best showed the complete auditory pathway (P < 0.01), whereas administration though the tympanum only demonstrated part of the pathway. The SI was highest at 24 h after administration of the tracer and began to decline at 48 h. The SI of the auditory cortex was higher after the injection of 0.4 M MnCl2than that of 0.2 M MnCl2. ME MRI best demonstrated the whole auditory pathway at 24 h after the injection of 0.4 M MnCl2through the perilymph in the rat, which provided an optimal method for the study of ME MRI of the auditory pathway in the animal model.
authors.affiliation: Department of Radiology, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Zuohua
authors.initials: Z
authors.lastname: Tang
authors.affiliation: Department of Otolaryngology, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Lingjie
authors.initials: L
authors.lastname: Wu
authors.affiliation: Department of Radiology, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Zebin
authors.initials: Z
authors.lastname: Xiao
authors.affiliation: Department of Radiology, Huashan Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Xiaoyuan
authors.initials: X
authors.lastname: Feng
authors.affiliation: Department of Ophthalmology, Eye and ENT Hospital of Shanghai Medical School, State Key Laboratory of Medical Neurobiology, Institutes of Brain Science, Fudan University, Shanghai, China.
authors.forename: Xinghuai
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Radiology, Huashan Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Tang
authors.affiliation: Department of Radiotherapy, Eye and ENT Hospital of Shanghai Medical School, Fudan University, Shanghai, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Siemens Ltd. Healthcare Sector, Shanghai, China.
authors.forename: Lixin
authors.initials: L
authors.lastname: Jin
mesh_terms: D000818:Animals; D001303:Auditory Cortex / Q000033:anatomy & histology*; D001306:Auditory Pathways / Q000033:anatomy & histology*; D002712:Chlorides; D003287:Contrast Media; D007089:Image Enhancement / Q000379:methods*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008297:Male; D017895:Manganese Compounds; D051381:Rats; D017207:Rats, Sprague-Dawley; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D002712:Chlorides; D003287:Contrast Media; D017895:Manganese Compounds; C025340:manganese chloride
keywords: MnCl2; auditory pathway; contrast-to-noise ratio (CNR); manganese-enhanced MRI (ME MRI); perilymph