title: [Investigation on the expression of microvessel pericyte coverage index and lymph vessel density in nasal polyps].
abstract: Objective:To investigate the role of lymph vessel density (LVD) and microvessel pericyte coverage index (MPI) in the pathogenesis of nasal polyps.Methods:Using immunohistochemistry and immunofluorescence double staining method, the expressions of albumin, D2-40 and CD34-α-SMA in 11 specimens of normal nasal mucosa, 26 specimens of nasal polyp and 26 specimens of inferior turbinate tissue from patients with nasal polyps were investigated. The counts of microvessel density (MVD), lymph vessel density (LVD) and microvessel pericyte coverage index (MPI) were compared. SPSS 17.0 software was used to analyze the data.Results:The nasal polyp group(0.269±0.096) had more albumin than inferior turbinate tissue group(0.159±0.078) and normal nasal mucosa group(0.138±0.045), the differences were significant (qvalue was 4.873, 4.446, bothP<0.05). The counts of MVD in nasal polyp group (30.52±4.42) were not significantly higher than those in inferior turbinate tissue group (30.33±6.03) and normal nasal mucosa group(28.21±6.84), the differences were not significant (qvalue was 0.130, 1.147, bothP>0.05). The MPI in nasal polyp group (0.291±0.096) was significantly lower than those in inferior turbinate tissue group(0.432±0.101) and normal nasal mucosa group(0.416±0.071), the difference was significant (qvalue was 5.399, 3.680, bothP<0.05). The counts of LVD in the nasal polyp group(0.245±0.073) were significantly lower than those in inferior turbinate tissue group (0.431±0.054) and normal nasal mucosa group(0.470±0.078), the difference was significant (qvalue was 10.004, 9.328, bothP<0.05). MPI expression in the nasal polyp group was negetively correlated to albumin expression(r=-0.889,P<0.05). The LVD expression in the nasal polyp group was negetively correlated to albumin expression(r=-0.901,P<0.05).Conclusion:Different LVD and MIP in nasal polyp tissues and normal nasal mucosa tissues imply that microcirculatory dysfunction plays a crucial role in the pathogenesis of nasal polyps.
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, China-Japan Unite Hospital of Jilin University, Changchun 130033, China.
authors.forename: N
authors.initials: N
authors.lastname: Cui
authors.forename: D D
authors.initials: DD
authors.lastname: Zhu
authors.forename: G M
authors.initials: GM
authors.lastname: Guan
authors.forename: J C
authors.initials: JC
authors.lastname: Sha
authors.forename: C D
authors.initials: CD
authors.lastname: Meng
authors.forename: L
authors.initials: L
authors.lastname: Li
authors.forename: Q
authors.initials: Q
authors.lastname: Xiu
mesh_terms: D000199:Actins / Q000378:metabolism; D000328:Adult; D000418:Albumins / Q000378:metabolism; D058846:Antibodies, Monoclonal, Murine-Derived / Q000378:metabolism; D018952:Antigens, CD34 / Q000378:metabolism; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D042601:Lymphatic Vessels / Q000378:metabolism / Q000473:pathology*; D008297:Male; D009297:Nasal Mucosa / Q000378:metabolism / Q000473:pathology*; D009298:Nasal Polyps / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D010446:Peptide Fragments / Q000378:metabolism; D020286:Pericytes / Q000378:metabolism / Q000473:pathology*; D014420:Turbinates / Q000378:metabolism / Q000473:pathology
chemical_list: D000199:Actins; D000418:Albumins; D058846:Antibodies, Monoclonal, Murine-Derived; D018952:Antigens, CD34; D010446:Peptide Fragments; C033025:actin subfragments; C500987:monoclonal antibody D2-40