title: Visualization of 5-HT Receptors Using Radioligand-Binding Autoradiography.
abstract: Described in this unit are techniques to visualize the majority of serotonin (5-hydroxytryptamine, 5-HT) receptor subtypes in sections of frozen brain tissue using receptor autoradiography. Protocols for brain extraction and sectioning, radioligand exposure, autoradiogram generation, and data quantification are provided, as are the optimal incubation conditions for the autoradiographic visualization of receptors using agonist and antagonist radioligands. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: IIBB-CSIC, IDIBAPS, CIBERNED, Barcelona, Spain.
authors.forename: Roser
authors.initials: R
authors.lastname: Cortés
authors.affiliation: IIBB-CSIC, IDIBAPS, CIBERNED, Barcelona, Spain.
authors.forename: M Teresa
authors.initials: MT
authors.lastname: Vilaró
authors.affiliation: IIBB-CSIC, IDIBAPS, CIBERNED, Barcelona, Spain.
authors.forename: Guadalupe
authors.initials: G
authors.lastname: Mengod
mesh_terms: D000818:Animals; D001345:Autoradiography / Q000379:methods*; D001923:Brain Chemistry; D006801:Humans; D011869:Radioligand Assay / Q000379:methods*; D011985:Receptors, Serotonin / Q000276:immunology* / Q000302:isolation & purification* / Q000378:metabolism; D012701:Serotonin / Q000737:chemistry / Q000378:metabolism; D012702:Serotonin Antagonists / Q000378:metabolism; D017366:Serotonin Receptor Agonists / Q000378:metabolism
chemical_list: D011985:Receptors, Serotonin; D012702:Serotonin Antagonists; D017366:Serotonin Receptor Agonists; D012701:Serotonin
keywords: 5-HT receptor subtypes; brain cryosections; brain tissue; radioligand binding; receptor autoradiography