title: Near-atomic-resolution cryo-EM analysis of the Salmonella T3S injectisome basal body.
abstract: The type III secretion (T3S) injectisome is a specialized protein nanomachine that is critical for the pathogenicity of many Gram-negative bacteria, including purveyors of plague, typhoid fever, whooping cough, sexually transmitted infections and major nosocomial infections. This syringe-shaped 3.5-MDa macromolecular assembly spans both bacterial membranes and that of the infected host cell. The internal channel formed by the injectisome allows for the direct delivery of partially unfolded virulence effectors into the host cytoplasm. The structural foundation of the injectisome is the basal body, a molecular lock-nut structure composed predominantly of three proteins that form highly oligomerized concentric rings spanning the inner and outer membranes. Here we present the structure of the prototypical Salmonella enterica serovar Typhimurium pathogenicity island 1 basal body, determined using single-particle cryo-electron microscopy, with the inner-membrane-ring and outer-membrane-ring oligomers defined at 4.3 Å and 3.6 Å resolution, respectively. This work presents the first, to our knowledge, high-resolution structural characterization of the major components of the basal body in the assembled state, including that of the widespread class of outer-membrane portals known as secretins.
authors.affiliation: Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: L J
authors.initials: LJ
authors.lastname: Worrall
authors.affiliation: CryoEM Shared Resources, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA.
authors.forename: C
authors.initials: C
authors.lastname: Hong
authors.affiliation: Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: M
authors.initials: M
authors.lastname: Vuckovic
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: W
authors.initials: W
authors.lastname: Deng
authors.affiliation: Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: J R C
authors.initials: JRC
authors.lastname: Bergeron
authors.affiliation: Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: D D
authors.initials: DD
authors.lastname: Majewski
authors.affiliation: CryoEM Shared Resources, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA.
authors.forename: R K
authors.initials: RK
authors.lastname: Huang
authors.affiliation: Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: T
authors.initials: T
authors.lastname: Spreter
authors.affiliation: Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia V6T 1Z4, Canada.
authors.forename: B B
authors.initials: BB
authors.lastname: Finlay
authors.affiliation: CryoEM Shared Resources, Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 20147, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Biochemistry and Molecular Biology and the Center for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
authors.forename: N C J
authors.initials: NCJ
authors.lastname: Strynadka