title: Fidelity of the DNA Ligase-Catalyzed Scaffolding of Peptide Fragments on Nucleic Acid Polymers.
abstract: We describe the development and analysis of the T4 DNA ligase-catalyzed DNA templated polymerization of pentanucleotides modified with peptide fragments toward the generation of ssDNA-scaffolded peptides. A high-throughput duplex DNA sequencing method was developed to facilitate the determination of fidelity for various codon sets and library sizes used during the polymerization process. With this process, we identified several codon sets that enable the efficient and sequence-specific incorporation of peptide fragments along a ssDNA template at fidelities up to 99% and with low sequence bias. These findings mark a significant advance in generating evolvable biomimetic polymers and should find ready application to the in vitro selection of molecular recognition.
authors.affiliation: Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602-2556, United States.
authors.forename: Chun
authors.initials: C
authors.lastname: Guo
authors.affiliation: Department of Chemistry, University of Georgia , 140 Cedar Street, Athens, Georgia 30602-2556, United States.
authors.forename: Ryan
authors.identifier: 0000-0002-8933-970X
authors.initials: R
authors.lastname: Hili
mesh_terms: D055162:Biocatalysis; D003062:Codon / Q000235:genetics; D011088:DNA Ligases / Q000378:metabolism*; D004277:DNA, Single-Stranded / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009690:Nucleic Acid Conformation; D010446:Peptide Fragments / Q000737:chemistry* / Q000378:metabolism; D058105:Polymerization; D013379:Substrate Specificity
chemical_list: D003062:Codon; D004277:DNA, Single-Stranded; D010446:Peptide Fragments; D011088:DNA Ligases