title: In vitro potential cytogenetic and oxidative stress effects of roxithromycin.
abstract: Macrolide antibiotic roxithromycin was evaluated in terms of its genotoxic, cytotoxic and oxidative stress effects. For this purpose; 25, 50, 100 and 200 μg/mL concentrations of roxithromycin were dissolved in dimethyl sulfoxide and treated to human peripheral blood lymphocytes for two different treatment periods (24 and 48 h). In chromosome aberration (CA) and micronucleus (MN) tests, roxithromycin did not show genotoxic effect. But it induced sister chromatid exchange (SCE) at the highest concentration (200 μg/mL) for the 24-h treatment period and at all concentrations (except 25 μg/mL) for the 48-h treatment period. Looking at cytotoxic effect of roxithromycin, statistically insignificant decreases on mitotic index and proliferation index were observed. Roxithromycin decreased nuclear division index (NDI) at highest two concentrations (100 and 200 μg/mL) for the 24-h treatment period and at all concentrations (expect 25 μg/mL) for the 48-h treatment period. Total oxidant values, total antioxidant values and oxidative stress index did not change with roxithromycin treatment. Eventually, roxithromycin did not have genotoxic and oxidative stress effects in human-cultured lymphocytes.
authors.affiliation: a Department of Nursing , School of Health Sciences, Ardahan University , Ardahan , Turkey and.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Arslan
authors.affiliation: b Department of Biology , Institute of Science, Cukurova University , Adana , Turkey.
authors.forename: Taygun
authors.identifier: http://orcid.org/0000-0002-5519-5744
authors.initials: T
authors.lastname: Timocin
authors.affiliation: b Department of Biology , Institute of Science, Cukurova University , Adana , Turkey.
authors.forename: Hasan B
authors.initials: HB
authors.lastname: Ila
mesh_terms: D000328:Adult; D000900:Anti-Bacterial Agents / Q000009:adverse effects*; D048750:Cell Nucleus Division / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002478:Cells, Cultured; D004260:DNA Repair / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D005260:Female; D006801:Humans; D008214:Lymphocytes / Q000166:cytology / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism; D008297:Male; D015162:Micronucleus Tests; D008940:Mitotic Index; D009152:Mutagenicity Tests; D009153:Mutagens / Q000009:adverse effects*; D009994:Osmolar Concentration; D018384:Oxidative Stress / Q000187:drug effects; D015575:Roxithromycin / Q000009:adverse effects*; D012854:Sister Chromatid Exchange / Q000187:drug effects*; D055815:Young Adult
chemical_list: D000900:Anti-Bacterial Agents; D009153:Mutagens; D015575:Roxithromycin
keywords: Roxithromycin; cytotoxicity; genotoxicity; oxidative stress