title: Variations in Chemical Composition, Vasorelaxant and Angiotensin I-Converting Enzyme Inhibitory Activities of Essential Oil from Aerial Parts of Seseli pallasii Besser (Apiaceae).
abstract: The present paper describes environmental and seasonal-related chemical composition variations, vasorelaxant and angiotensin I-converting enzyme (ACE) activities of essential oil from aerial parts of Seseli pallasii Besser. The composition was analyzed by GC and GC/MS. Monoterpenes were found to be the most abundant chemical class with α-pinene (42.7 - 48.2%) as the most prevalent component. Seseli pallasi essential oil relaxed isolated endothelium-intact mesenteric arteries rings precontracted with phenylephrine with IC50= 3.10 nl/ml (IC50= 2.70 μg/ml). Also, S. pallasii essential oil was found to exhibit a dose-dependent ACE inhibitory activity with an IC50value of 0.33 mg/ml. In silico evaluation of ACE inhibitory activity of the individual components showed that spathulenol exhibited the best binding affinity with ACE, and the lowest binding energy of -7.5 kcal/mol. The results suggested that combination of vasorelaxing and ACE inhibitory effects of the analyzed S. pallasii essential oil might have the potential therapeutic significance in hypertension.
authors.affiliation: Department of Pharmacognosy, University of Banja Luka-Faculty of Medicine, Save Mrkalja 14, 78000, Banjaluka, Bosnia and Herzegovina.
authors.forename: Relja
authors.initials: R
authors.lastname: Suručić
authors.affiliation: Department of Pharmacognosy, University of Belgrade-Faculty of Pharmacy, V.Stepe 450, Belgrade, Serbia.
authors.forename: Tatjana
authors.initials: T
authors.lastname: Kundaković
authors.affiliation: Department of Botany, University of Belgrade-Faculty of Pharmacy, V.Stepe 450, Belgrade, Serbia.
authors.forename: Branislava
authors.initials: B
authors.lastname: Lakušić
authors.affiliation: Faculty of Medicine, University of Eastern Sarajevo, Studentska 7, Foča, Bosnia and Herzegovina.
authors.forename: Dragana
authors.initials: D
authors.lastname: Drakul
authors.affiliation: Faculty of Medicine, University of Eastern Sarajevo, Studentska 7, Foča, Bosnia and Herzegovina.
authors.forename: Slobodan R
authors.initials: SR
authors.lastname: Milovanović
authors.affiliation: Department of Pharmacognosy, University of Belgrade-Faculty of Pharmacy, V.Stepe 450, Belgrade, Serbia.
authors.forename: Nada
authors.initials: N
authors.lastname: Kovačević
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D019661:Apiaceae / Q000737:chemistry*; D000081008:Bicyclic Monoterpenes; D008401:Gas Chromatography-Mass Spectrometry; D008297:Male; D008638:Mesenteric Arteries / Q000187:drug effects; D062105:Molecular Docking Simulation; D039821:Monoterpenes / Q000032:analysis / Q000302:isolation & purification; D009822:Oils, Volatile / Q000737:chemistry*; D035261:Plant Components, Aerial / Q000737:chemistry; D051381:Rats; D017208:Rats, Wistar; D012621:Seasons; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000494:pharmacology; D014665:Vasodilator Agents / Q000302:isolation & purification / Q000494:pharmacology*
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D012717:Sesquiterpenes; D014665:Vasodilator Agents; C013258:spathulenol; C005451:alpha-pinene
keywords: 
  Seseli pallasii
  ; Angiotensin I-converting enzyme inhibitory activities; Essential oils composition variations; Molecular docking; Vasorelaxant activities