title: Characterization of a Porous Nano-electrospray Capillary Emitter at Ultra-low Flow Rates.
abstract: Biopharmaceuticals, especially therapeutic monoclonal antibodies, have emerged as a very promising new generation of protein-based drugs. However, their comprehensive analysis continues to pose new challenges for the bioanalytical field. Hyphenation of capillary electrophoresis with electrospray ionization (CE-MS) is a promising technique to address these challenges. One of the main advantages of CE-MS is the ability to produce stable electrospray at ultra-low flow rates (5-20 nl/min range). In this short communication we report on the characterization of a porous nano-electrospray capillary emitter focusing on the effects of ultra-low flow rate on ionization efficiency, ion suppression and detection sensitivity. Ion suppression effect of a poorly-ionizable sugar in the presence of an easily-ionized peptide was reduced by almost 2-fold. Intact therapeutic antibody infusion analysis demonstrated that MS detection sensitivity increased by an order of magnitude with the decrease of flow rate from 250 nL/min to 20 nL/min using the nano-electrospray capillary emitter.
authors.affiliation: The Scripps Research Institute , La Jolla, CA, USA.|CEITEC - Central European Institute of Technology , Brno, Czech Republic.
authors.forename: Gabor
authors.initials: G
authors.lastname: Jarvas
authors.affiliation: The Scripps Research Institute , La Jolla, CA, USA.|Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic , Czech Republic.
authors.forename: Bryan
authors.initials: B
authors.lastname: Fonslow
authors.affiliation: The Scripps Research Institute , La Jolla, CA, USA.
authors.forename: John R
authors.initials: JR
authors.lastname: Yates
authors.affiliation: CEITEC - Central European Institute of Technology , Brno, Czech Republic.|Institute of Analytical Chemistry of the Academy of Sciences of the Czech Republic , Czech Republic.
authors.forename: Frantisek
authors.initials: F
authors.lastname: Foret
authors.affiliation: MTA-PE Translational Glycomics Group, University of Pannonia, Veszprem, Hungary a.guttman@northeastern.edu.
authors.forename: Andras
authors.initials: A
authors.lastname: Guttman
mesh_terms: D000911:Antibodies, Monoclonal / Q000032:analysis* / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D000911:Antibodies, Monoclonal