title: SingleRGene Introgression Lines for Accurate Dissection of theBrassica-LeptosphaeriaPathosystem.
abstract: Seven blackleg resistance (R) genes (Rlm1, Rlm2, Rlm3, Rlm4, LepR1, LepR2&LepR3) were each introgressed into a common susceptibleB. napusdoubled-haploid (DH) line through reciprocal back-crossing, producing single-Rgene introgression lines (ILs) for use in the pathological and molecular study ofBrassica-Leptosphaeriainteractions. The genomic positions of theRgenes were defined through molecular mapping and analysis with transgenicL. maculansisolates was used to confirm the identity of the introgressed genes where possible. UsingL. maculansisolates of contrasting avirulence gene (Avr) profiles, we preformed extensive differential pathology for phenotypic comparison of the ILs to otherB. napusvarieties, demonstrating the ILs can provide for the accurate assessment ofAvr-Rgene interactions by avoiding non-Avrdependant alterations to resistance responses which can occur in some commonly usedB. napusvarieties. Whole-genome SNP-based assessment allowed us to define the donor parent introgressions in each IL and provide a strong basis for comparative molecular dissection of the pathosystem.
authors.affiliation: Saskatoon Research Centre, Agriculture and Agri-Food CanadaSaskatoon, SK, Canada; Armatus Genetics Inc.Saskatoon, SK, Canada.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Larkan
authors.affiliation: Saskatoon Research Centre, Agriculture and Agri-Food Canada Saskatoon, SK, Canada.
authors.forename: Fengqun
authors.initials: F
authors.lastname: Yu
authors.affiliation: Saskatoon Research Centre, Agriculture and Agri-Food Canada Saskatoon, SK, Canada.
authors.forename: Derek J
authors.initials: DJ
authors.lastname: Lydiate
authors.affiliation: Saskatoon Research Centre, Agriculture and Agri-Food Canada Saskatoon, SK, Canada.
authors.forename: S Roger
authors.initials: SR
authors.lastname: Rimmer
authors.affiliation: Saskatoon Research Centre, Agriculture and Agri-Food Canada Saskatoon, SK, Canada.
authors.forename: M Hossein
authors.initials: MH
authors.lastname: Borhan
keywords: Brassica napus; Leptosphaeria maculans; blackleg; differentical lines; introgression lines; resistance genes