title: Analysis of plant hormone profiles in response to moderate dehydration stress.
abstract: Plant responses to dehydration stress are mediated by highly complex molecular systems involving hormone signaling and metabolism, particularly the major stress hormone abscisic acid (ABA) and ABA-dependent gene expression. To understand the roles of plant hormones and their interactions during dehydration, we analyzed the plant hormone profiles with respect to dehydration responses in Arabidopsis thaliana wild-type (WT) plants and ABA biosynthesis mutants (nced3-2). We developed a procedure for moderate dehydration stress, and then investigated temporal changes in the profiles of ABA, jasmonic acid isoleucine (JA-Ile), salicylic acid (SA), cytokinin (trans-zeatin, tZ), auxin (indole-acetic acid, IAA), and gibberellin (GA4), along with temporal changes in the expression of key genes involved in hormone biosynthesis. ABA levels increased in a bi-phasic pattern (at the early and late phases) in response to moderate dehydration stress. JA-Ile levels increased slightly in WT plants and strongly increased in nced3-2 mutant plants at 72 h after the onset of dehydration. The expression profiles of dehydration-inducible genes displayed temporal responses in an ABA-dependent manner. The early phase of ABA accumulation correlated with the expression of touch-inducible genes and was independent of factors involved in the major ABA regulatory pathway, including the ABA-responsive element-binding (AREB/ABF) transcription factor. JA-Ile, SA, and tZ were negatively regulated during the late dehydration response phase. Transcriptome analysis revealed important roles for hormone-related genes in metabolism and signaling during dehydration-induced plant responses.
authors.affiliation: RIKEN Center for Sustainable Resource Science (CSRS), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Urano
authors.affiliation: Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki, 305-8686, Japan.
authors.forename: Kyonoshin
authors.initials: K
authors.lastname: Maruyama
authors.affiliation: RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa, 230-0045, Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Jikumaru
authors.affiliation: RIKEN Center for Sustainable Resource Science (CSRS), 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Kamiya
authors.affiliation: Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Kazuko
authors.initials: K
authors.lastname: Yamaguchi-Shinozaki
authors.affiliation: RIKEN Center for Sustainable Resource Science (CSRS), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shinozaki
mesh_terms: D000040:Abscisic Acid / Q000378:metabolism; D017360:Arabidopsis / Q000378:metabolism / Q000502:physiology; D029681:Arabidopsis Proteins / Q000378:metabolism; D003517:Cyclopentanes / Q000378:metabolism; D003681:Dehydration; D049308:Dioxygenases / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D054883:Oxylipins / Q000378:metabolism; D010937:Plant Growth Regulators / Q000378:metabolism*; D010940:Plant Proteins / Q000378:metabolism; D015398:Signal Transduction; D014157:Transcription Factors
chemical_list: D029681:Arabidopsis Proteins; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D010940:Plant Proteins; D014157:Transcription Factors; C011006:jasmonic acid; D000040:Abscisic Acid; D049308:Dioxygenases; C109043:9-cis-epoxy-carotenoid dioxygenase
keywords: 9-cis-epoxycarotenoid dioxygenase 3; abscisic acid; dehydration stress; hormone profiling; transcriptomics