title: Intrafibrillar mineralization of polyacrylic acid-bound collagen fibrils using a two-dimensional collagen model and Portland cement-based resins.
abstract: The biomimetic remineralization of apatite-depleted dentin is a potential method for enhancing the durability of resin-dentin bonding. To advance this strategy from its initial proof-of-concept design, we sought to investigate the characteristics of polyacrylic acid (PAA) adsorption to desorption from type I collagen and to test the mineralization ability of PAA-bound collagen. Portland cement and β-tricalcium phosphate (β-TCP) were homogenized with a hydrophilic resin blend to produce experimental resins. The collagen fibrils reconstituted on nickel (Ni) grids were mineralized using different methods: (i) group I consisted of collagen treated with Portland cement-based resin in simulated body fluid (SBF); (ii) group II consisted of PAA-bound collagen treated with Portland cement-based resin in SBF; and (iii) group III consisted of PAA-bound collagen treated with β-TCP-doped Portland cement-based resin in deionized water. Intrafibrillar mineralization was evaluated using transmission electron microscopy. We found that a carbonyl-associated peak at pH 3.0 increased as adsorption time increased, whereas a hydrogen bond-associated peak increased as desorption time increased. The experimental resins maintained an alkaline pH and the continuous release of calcium ions. Apatite was detected within PAA-bound collagen in groups II and III. Our results suggest that PAA-bound type I collagen fibrils can be mineralized using Portland cement-based resins.
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Wu
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Lisha
authors.initials: L
authors.lastname: Gu
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Zihua
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Qiurong
authors.initials: Q
authors.lastname: Sun
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Huimin
authors.initials: H
authors.lastname: Chen
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Junqi
authors.initials: J
authors.lastname: Ling
authors.affiliation: Guangdong Provincial Key Laboratory of Stomatology, Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Sui
authors.initials: S
authors.lastname: Mai
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000327:Adsorption; D032701:Biomimetics; D002130:Calcium Phosphates / Q000737:chemistry; D024042:Collagen Type I / Q000737:chemistry*; D001840:Dental Bonding; D003738:Dental Cements / Q000737:chemistry*; D008422:Materials Testing; D046529:Microscopy, Electron, Transmission; D009532:Nickel / Q000737:chemistry; D019279:Resin Cements / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D000180:Acrylic Resins; D002130:Calcium Phosphates; D024042:Collagen Type I; D003738:Dental Cements; D019279:Resin Cements; C485817:beta-tricalcium phosphate; C006903:carbopol 940; D009532:Nickel
keywords: biomimetics; dentin; type I collagen