title: The measurement of cutaneous blood flow in healthy volunteers subjected to physical exercise with ultrasound Doppler imaging and laser Doppler flowmetry.
abstract: We aimed to evaluate hemodynamic parameters in the cutaneous microcirculation during recovery to graded dynamic exercise performed on a cycloergometer by using two distinctive methods: high-frequency ultrasound Doppler flowmetry (UDF) and laser Doppler fluxmetry (LDF). Regarding UDF, the medium linear blood flow velocity (Vam) was estimated, expressed in absolute units (cm/s) whereas blood flow assessed by LDF was registered in arbitrary perfusion units (PU). We compared the corresponding perfusion values obtained by UDF and LDF, respectively, every 20 s during recovery period to physical exercise. Three types of microvascular blood flow dynamics were recorded: blood flow with an occasional paroxysm, a pulsatile blood flow pattern, and a shunting-type blood flow pattern. In the first type, the reaction time of registration and the direction of changes of UDF and LDF values coincided in 77.7%, exhibiting high correlation (r = 0.77; p≤0.001). In the second type, the direction of changes of UDF and LDF showed 85.0% similarity and a moderate correlation (r = 0.66; p = 0.0015), whereas in the third type, there was also a moderate correlation (r = 0.53, p = 0.0024). Our pilot experiments have shown that UDF could be regarded as a comparable substitutional method to LDF for studying skin microcirculation.
authors.affiliation: Institute of Physiology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Helena
authors.initials: H
authors.lastname: Lenasi
authors.affiliation: Institute of Physiology, Medical Faculty, University of Ljubljana, Ljubljana, Slovenia.
authors.forename: Nejka
authors.initials: N
authors.lastname: Potočnik
authors.affiliation: Saint-Petersburg I.P. Pavlov State Medical University (PSPbGMU), St. Petersburg, Russia.
authors.forename: Nikolaj
authors.initials: N
authors.lastname: Petrishchev
authors.affiliation: North-West State Medical University, L.G. Sokolov Clinical Hospital No122, St. Petersburg, Russia.
authors.forename: Marina
authors.initials: M
authors.lastname: Papp
authors.affiliation: SPMinimaxLtd, St. Petersburg, Russia.
authors.forename: Anastasia
authors.initials: A
authors.lastname: Egorkina
authors.affiliation: SPMinimaxLtd, St. Petersburg, Russia.
authors.forename: Marina
authors.initials: M
authors.lastname: Girina
authors.affiliation: State Scientific Center of the Russian Federation Institute of Biomedical Problems RAS, Moscow, Russia.
authors.forename: Marina
authors.initials: M
authors.lastname: Skedina
authors.affiliation: State Scientific Center of the Russian Federation Institute of Biomedical Problems RAS, Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Kovaleva
mesh_terms: D000328:Adult; D015444:Exercise / Q000502:physiology*; D005260:Female; D064368:Healthy Volunteers; D006801:Humans; D017078:Laser-Doppler Flowmetry / Q000379:methods*; D008297:Male; D010865:Pilot Projects; D012039:Regional Blood Flow / Q000502:physiology*; D014463:Ultrasonography / Q000379:methods*; D055815:Young Adult
keywords: Microcirculation; high-frequency Doppler sonography; laser Doppler fluxmetry; physical exercise; ultrasound Doppler flowmetry