title: Pectin from Opuntia ficus indica: Optimization of microwave-assisted extraction and preliminary characterization.
abstract: Optimization of microwave-assisted extraction (MAE) of water-soluble pectin (WSP) from Opuntia ficus indica cladodes was performed using Response Surface Methodology. The effect of extraction time (X1), microwave power (X2), pH (X3) and solid-to-liquid ratio (X4) on the extraction yield was examined. The optimum conditions of MAE were as follows: X1=2.15min; X2=517W; X3=2.26 and X4=2g/30.6mL. The maximum obtained yield of pectin extraction was 12.57%. Total carbohydrate content of WSP is about 95.5% including 34.4% of Galacturonic acid. Pectin-related proteins represent only the 0.66% of WSP mass. HPSEC and light scattering analyses reveal that WSP is mostly constituted of high molecular pectin and FTIR measurements show that the microwave treatment does not alter the chemical structure of WSP, in which Galacturonic acid content and yield are 34.4% and 4.33%, respectively. Overall, application of MAE can give rise to high quality pectin.
authors.affiliation: Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria; Département de Biochimie et Microbiologie, Faculté des Sciences Biologiques et des Sciences Agronomiques, Université de Tiziouzou, 15000 Tizi ouzou, Algeria. Electronic address: klefsih@yahoo.fr.
authors.forename: Khalef
authors.initials: K
authors.lastname: Lefsih
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors.forename: Daniela
authors.initials: D
authors.lastname: Giacomazza
authors.affiliation: Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Farid
authors.initials: F
authors.lastname: Dahmoune
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors.forename: Maria Rosalia
authors.initials: MR
authors.lastname: Mangione
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors.forename: Donatella
authors.initials: D
authors.lastname: Bulone
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: San Biagio
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors.forename: Rosa
authors.initials: R
authors.lastname: Passantino
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors.forename: Maria Assunta
authors.initials: MA
authors.lastname: Costa
authors.affiliation: Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy.
authors.forename: Valeria
authors.initials: V
authors.lastname: Guarrasi
authors.affiliation: Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000 Bejaia, Algeria.
authors.forename: Khodir
authors.initials: K
authors.lastname: Madani
mesh_terms: D006603:Hexuronic Acids / Q000032:analysis; D008872:Microwaves; D029747:Opuntia / Q000737:chemistry*; D010368:Pectins / Q000032:analysis*
chemical_list: D006603:Hexuronic Acids; C007819:galacturonic acid; D010368:Pectins
keywords: FTIR; HPSEC; Light scattering; Microwave-assisted extraction; Pectin; Surface Response Methodology