title: Antibacterial activity of agricultural waste derived wollastonite doped with copper for bone tissue engineering.
abstract: Bioactive ceramic materials with metal ions generation brought great attention in the class of biomaterials development and widely employed as a filler material for bone tissue regeneration. The present study aimed to fabricate calcium silicate based ceramic material doped with copper metal particles by sol-gel method. Rice straw of agricultural waste was utilized as a source material to synthesize wollastonite, then wollastonite was doped with copper to fabricate copper doped wollastonite (Cu-Ws) particles. The synthesized materials were subjected to physio-chemical characterization by TEM, DLS, FTIR, XRD and DSC analysis. It was found that the sizes of the WS particles was around 900nm, while adding copper the size was increased upto 1184nm and the addition of copper to the material sharpening the peak. The release of Cu ions was estimated by ICP analysis. The anti-bacterial potentiality of the particles suggested that better microbial growth inhibition against E. coli (Gram negative) and S. aureus (Gram positive) strains from ATCC, in which the growth inhibition was more significant against S. aureus. The biocompatibility in mouse Mesenchymal Stem cells (mMSC) showed the non-toxic effect up to 0.05mg/ml concentration while the increase in concentration was found to be toxic to the cells. So the particles may have better potential application with the challenging prevention of post implantation infection in the field of bone tissue engineering (BTE).
authors.affiliation: Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Tamil Nadu 603 103, India.
authors.forename: S
authors.initials: S
authors.lastname: Azeena
authors.affiliation: Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Tamil Nadu 603 103, India.
authors.forename: N
authors.initials: N
authors.lastname: Subhapradha
authors.affiliation: Tissue Engineering and Cancer Research Laboratory, Department of Biotechnology, SRM University, Kattankulathur, Tamil Nadu 603 103, India.
authors.forename: N
authors.initials: N
authors.lastname: Selvamurugan
authors.affiliation: Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Tamil Nadu 603 103, India.
authors.forename: S
authors.initials: S
authors.lastname: Narayan
authors.affiliation: Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Tamil Nadu 603 103, India.
authors.forename: N
authors.initials: N
authors.lastname: Srinivasan
authors.affiliation: Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Tamil Nadu 603 103, India.
authors.forename: R
authors.initials: R
authors.lastname: Murugesan
authors.affiliation: Tissue Engineering and Drug Delivery Laboratory, Department of Biomedical Engineering, National Yang Ming University, Taipei 112, Taiwan, ROC.
authors.forename: T W
authors.initials: TW
authors.lastname: Chung
authors.affiliation: Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Chettinad Health City, Kelambakkam, Tamil Nadu 603 103, India. Electronic address: moorthiibms@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Moorthi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D001861:Bone Regeneration / Q000187:drug effects; D018786:Bone Substitutes / Q000737:chemistry / Q000494:pharmacology; D017610:Calcium Compounds / Q000737:chemistry / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology; D004926:Escherichia coli / Q000254:growth & development; D008422:Materials Testing; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D051379:Mice; D017640:Silicates / Q000737:chemistry / Q000494:pharmacology; D013211:Staphylococcus aureus / Q000254:growth & development; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D000900:Anti-Bacterial Agents; D018786:Bone Substitutes; D017610:Calcium Compounds; D017640:Silicates; D003300:Copper; C031293:calcium silicate
keywords: Agricultural waste; Bone tissue engineering; Copper doped wollastonite; E. coli; S. aureus; Wollastonite