title: Transcriptome portrait of cellulose-enriched flax fibres at advanced stage of specialization.
abstract: Functional specialization of cells is among the most fundamental processes of higher organism ontogenesis. The major obstacle to studying this phenomenon in plants is the difficulty of isolating certain types of cells at defined stages of in planta development for in-depth analysis. A rare opportunity is given by the developed model system of flax (Linum usitatissimum L.) phloem fibres that can be purified from the surrounding tissues at the stage of the tertiary cell wall deposition. The performed comparison of the whole transcriptome profile in isolated fibres and other portions of the flax stem, together with fibre metabolism characterization, helped to elucidate the general picture of the advanced stage of plant cell specialization and to reveal novel participants potentially involved in fibre metabolism regulation and cell wall formation. Down-regulation of all genes encoding proteins involved in xylan and lignin synthesis and up-regulation of genes for the specific set of transcription factors transcribed during tertiary cell wall formation were revealed. The increased abundance of transcripts for several glycosyltransferases indicated the enzymes that may be involved in synthesis of fibre-specific version of rhamnogalacturonan I.
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Science, Lobachevsky str., 2/31, Kazan, 420111, Russia.
authors.forename: Oleg
authors.initials: O
authors.lastname: Gorshkov
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Science, Lobachevsky str., 2/31, Kazan, 420111, Russia.
authors.forename: Natalia
authors.initials: N
authors.lastname: Mokshina
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Science, Lobachevsky str., 2/31, Kazan, 420111, Russia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Gorshkov
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Science, Lobachevsky str., 2/31, Kazan, 420111, Russia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Chemikosova
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Science, Lobachevsky str., 2/31, Kazan, 420111, Russia.
authors.forename: Yuri
authors.initials: Y
authors.lastname: Gogolev
authors.affiliation: Kazan Institute of Biochemistry and Biophysics, Kazan Scientific Centre, Russian Academy of Science, Lobachevsky str., 2/31, Kazan, 420111, Russia. gorshkova@kibb.knc.ru.
authors.forename: Tatyana
authors.identifier: http://orcid.org/0000-0003-0342-8195
authors.initials: T
authors.lastname: Gorshkova
mesh_terms: D002245:Carbon Dioxide / Q000378:metabolism; D002250:Carbon Radioisotopes / Q000378:metabolism; D002454:Cell Differentiation / Q000235:genetics; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D019597:Flax / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling / Q000379:methods; D018506:Gene Expression Regulation, Plant; D010368:Pectins / Q000378:metabolism; D052585:Phloem / Q000166:cytology / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D018547:Plant Stems / Q000166:cytology / Q000235:genetics / Q000378:metabolism; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D002250:Carbon Radioisotopes; D010940:Plant Proteins; C042491:rhamnogalacturonan I; D002245:Carbon Dioxide; D010368:Pectins; D002482:Cellulose
keywords: Cellulose; G-layer; Glycosyltransferases; Linum usitatissimum (flax); Phloem fibres; Rhamnogalacturonan I; Tertiary cell wall; Transcription factors; Transcriptome