title: Vitamin D Effects on Osteoblastic Differentiation of Mesenchymal Stem Cells from Dental Tissues.
abstract: 1α,25-Dihydroxyvitamin D3(1,25(OH)2D3), the active metabolite of vitamin D (Vit D), increases intestinal absorption of calcium and phosphate, maintaining a correct balance of bone remodeling. Vit D has an anabolic effect on the skeletal system and is key in promoting osteoblastic differentiation of human Mesenchymal Stem Cells (hMSCs) from bone marrow. MSCs can be also isolated from the immature form of the tooth, the dental bud: Dental Bud Stem Cells (DBSCs) are adult stem cells that can effectively undergo osteoblastic differentiation. In this work we investigated the effect of Vit D on DBSCs differentiation into osteoblasts. Our data demonstrate that DBSCs, cultured in an opportune osteogenic medium, differentiate into osteoblast-like cells; Vit D treatment stimulates their osteoblastic features, increasing the expression of typical markers of osteoblastogenesis like RUNX2 and Collagen I (Coll I) and, in a more important way, determining a higher production of mineralized matrix nodules.
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
authors.forename: Francesca
authors.identifier: 0000-0001-8099-3505
authors.initials: F
authors.lastname: Posa
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
authors.forename: Adriana
authors.initials: A
authors.lastname: Di Benedetto
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, Italy.
authors.forename: Graziana
authors.identifier: 0000-0001-8501-5399
authors.initials: G
authors.lastname: Colaianni
authors.affiliation: Institute of Physical Chemistry, Department of Biophysical Chemistry, University of Heidelberg, Heidelberg, Germany; Max Planck Institute for Intelligent Systems, Stuttgart, Germany.
authors.forename: Elisabetta A
authors.initials: EA
authors.lastname: Cavalcanti-Adam
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, Italy.
authors.forename: Giacomina
authors.identifier: 0000-0002-0681-1432
authors.initials: G
authors.lastname: Brunetti
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
authors.forename: Chiara
authors.initials: C
authors.lastname: Porro
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Trotta
authors.affiliation: Section of Human Anatomy and Histology, Department of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Grano
authors.affiliation: Department of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, Italy.
authors.forename: Giorgio
authors.identifier: 0000-0001-6731-0247
authors.initials: G
authors.lastname: Mori