title: The effect of poly(ADP-ribosyl)ation inhibition on the porcine cumulus-oocyte complex during in vitro maturation.
abstract: Poly(ADP-ribosyl)ation (PARylation) plays important roles in DNA repair, apoptosis, transcriptional regulation, and cell death, and occurs via the activity of poly(ADP-ribose) polymerases (PARPs). Previous studies have shown that PARylation affects mouse and porcine pre-implantation development and participates in mechanisms of autophagy. However, there have not yet been reported the role of PARylation during in vitro maturation (IVM) of porcine oocytes. Thus, we investigated the effect of PARylation inhibition on this process; cumulus-oocyte complexes (COCs) were cultured with 3-aminobenzamide (3-ABA, PARP inhibitor) during porcine IVM. Full cumulus expansion was significantly reduced (10.34 ± 1.23 [3-ABA] vs. 48.17 ± 2.03% [control]), but nuclear maturation rates were not changed in the 3-ABA treatment group. Especially, we observed that cumulus cells were little expanded after 22 h in 3-ABA treated COCs. The mRNA expression levels of oocyte maturation- and cumulus expansion-related genes were evaluated at 22 and 44 h. GDF9, BMP15, COX-2, and PTX3 expression were upregulated at 44 h, whereas the levels of HAS2 and TNFAIP6 were downregulated in the 3-ABA treated group. Furthermore, 3-ABA treatment significantly decreased the developmental rate (28.24 ± 1.06 vs. 40.24 ± 3.03%) and total cell number (41.12 ± 2.10 vs. 50.38 ± 2.27), but increased the total apoptotic index (6.44 ± 0.81 vs. 3.08 ± 0.51) in parthenogenetically activated embryos. In conclusion, these results showed that PARylation regulates cumulus expansion through the regulation of gene expression and affects developmental competence and quality in parthenogenetic embryos.
authors.affiliation: Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul, South Korea.
authors.forename: Duk Hyoun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: CHA Fertility Center Seoul, Jung-gu, Seoul, South Korea.
authors.forename: Hye Ran
authors.initials: HR
authors.lastname: Lee
authors.affiliation: Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul, South Korea.
authors.forename: Min Gyeong
authors.initials: MG
authors.lastname: Kim
authors.affiliation: Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul, South Korea.
authors.forename: Jun Sung
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul, South Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Jin
authors.affiliation: Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul, South Korea. Electronic address: htl3675@konkuk.ac.kr.
authors.forename: Hoon Taek
authors.initials: HT
authors.lastname: Lee
mesh_terms: D000818:Animals; D001549:Benzamides / Q000494:pharmacology; D054885:Cumulus Cells / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D015870:Gene Expression; D059471:In Vitro Oocyte Maturation Techniques / Q000379:methods*; D009865:Oocytes / Q000187:drug effects / Q000201:enzymology / Q000502:physiology*; D010312:Parthenogenesis / Q000187:drug effects / Q000235:genetics / Q000502:physiology*; D011061:Poly A / Q000037:antagonists & inhibitors; D000067856:Poly(ADP-ribose) Polymerase Inhibitors / Q000494:pharmacology; D011065:Poly(ADP-ribose) Polymerases / Q000378:metabolism*; D013552:Swine
chemical_list: D001549:Benzamides; D000067856:Poly(ADP-ribose) Polymerase Inhibitors; D011061:Poly A; C025160:3-aminobenzamide; D011065:Poly(ADP-ribose) Polymerases
keywords: 3-aminobenzamide; Cumulus expansion; In vitro maturation; PARylation; Pig; Poly(ADP-ribosyl)ation