title: Functional single nucleotide polymorphisms of matrix metalloproteinase 7 and 12 genes in idiopathic recurrent spontaneous abortion.
abstract: OBJECTIVE
  The aim of this study was to investigate the potential association of matrix metalloproteinase 7 (MMP7) -181 A/G and MMP12 -82 A/G functional single nucleotide polymorphisms (SNP) with idiopathic recurrent spontaneous abortion (IRSA) in Slovenian reproductive couples.
  
  
  METHODS
  A case-control study was conducted on 149 couples with 3 or more consecutive idiopathic spontaneous pregnancy loses and 149 women and men with at least 2 live births and no history of pregnancy complications. Genotyping of MMP7 -181 A/G and MMP12 -82 A/G SNPs was performed using polymerase chain reaction and restriction fragment length polymorphism methods.
  
  
  RESULTS
  There were no statistically significant differences in the distribution of MMP7 -181 A/G and MMP12 -82 A/G genotype, allele, or haplotype frequencies between IRSA patients and controls, as well as patients' primary and secondary IRSA. We also found no association of MMP7 -181 A/G and MMP12 -82 A/G genotypes, alleles, and haplotypes with IRSA.
  
  
  CONCLUSIONS
  We found no evidence to support the association between IRSA and MMP7 -181 A/G and MMP12 -82 A/G SNPs in Slovenian reproductive couples.
authors.affiliation: Department of Biology and Medical Genetics, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia.
authors.forename: Anita
authors.initials: A
authors.lastname: Barišić
authors.affiliation: Department of Biology and Medical Genetics, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia.
authors.forename: Nina
authors.initials: N
authors.lastname: Pereza
authors.affiliation: Clinical Institute of Medical Genetics, Department of Gynaecology and Obstetrics, UMC Ljubljana, 1000, Ljubljana, Slovenia.
authors.forename: Alenka
authors.initials: A
authors.lastname: Hodžić
authors.affiliation: Department of Biology and Medical Genetics, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia.
authors.forename: Miljenko
authors.initials: M
authors.lastname: Kapović
authors.affiliation: Clinical Institute of Medical Genetics, Department of Gynaecology and Obstetrics, UMC Ljubljana, 1000, Ljubljana, Slovenia.
authors.forename: Borut
authors.initials: B
authors.lastname: Peterlin
authors.affiliation: Department of Biology and Medical Genetics, Faculty of Medicine, University of Rijeka, B. Branchetta 20, 51000, Rijeka, Croatia. sasa.ostojic@medri.uniri.hr.
authors.forename: Saša
authors.identifier: http://orcid.org/0000-0001-7134-4335
authors.initials: S
authors.lastname: Ostojić
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics* / Q000473:pathology; D000022:Abortion, Spontaneous / Q000235:genetics* / Q000473:pathology; D000328:Adult; D000483:Alleles; D005260:Female; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D053508:Matrix Metalloproteinase 12 / Q000235:genetics*; D020783:Matrix Metalloproteinase 7 / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011247:Pregnancy
chemical_list: C060932:MMP7 protein, human; D020783:Matrix Metalloproteinase 7; C507122:MMP12 protein, human; D053508:Matrix Metalloproteinase 12
keywords: Matrix metalloproteinases; Pregnancy; Recurrent spontaneous abortion; Single nucleotide polymorphisms