title: Visual colorimetric sensor array for discrimination of antioxidants in serum using MnO2nanosheets triggered multicolor chromogenic system.
abstract: Here we report a unique visual colorimetric sensor array for discrimination of antioxidants in serum based on MnO2nanosheets-3,3',5,5'-tetramethylbenzidine (TMB) multicolor chromogenic system. The absorbance values of the system at 370, 450, and 650nm provide three cross-reactive sensing elements. The presence of antioxidant will inhibit the reaction between TMB and MnO2nanosheets due to the presence of the competitive reaction of MnO2nanosheets and antioxidants. Different antioxidants containing uric acid, glutathione, ascorbic acid, cysteine, and melatonin have distinct reducing ability, producing a differential inhibition of MnO2nanosheets-TMB system, and therefore generating distinct colorimetric response patterns at 370, 450, and 650nm. The obtained patterns for each antioxidant at a concentration of 20μM were successfully discriminated using principal component analysis both in buffer and when spiked into fetal bovine serum (FBS). The identification accuracy of 45 unknown samples was found to be 100%. Remarkably, this sensor assay can visually discriminate antioxidants in diluted FBS with the naked eye.
authors.affiliation: School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, PR China.
authors.forename: Yuequan
authors.initials: Y
authors.lastname: Deng
authors.affiliation: School of National Defence Science & Technology, Southwest University of Science and Technology, Mianyang 621010, PR China. Electronic address: yhe2014@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: He
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis*; D001205:Ascorbic Acid / Q000032:analysis / Q000097:blood; D001560:Benzidines / Q000737:chemistry*; D015374:Biosensing Techniques; D002417:Cattle; D002863:Chromogenic Compounds / Q000737:chemistry*; D003124:Colorimetry / Q000379:methods*; D003545:Cysteine / Q000032:analysis / Q000097:blood; D005978:Glutathione / Q000032:analysis / Q000097:blood; D017895:Manganese Compounds / Q000737:chemistry*; D008550:Melatonin / Q000032:analysis / Q000097:blood; D049329:Nanostructures / Q000737:chemistry*; D010087:Oxides / Q000737:chemistry*; D044967:Serum / Q000737:chemistry; D014527:Uric Acid / Q000032:analysis / Q000097:blood
chemical_list: D000975:Antioxidants; D001560:Benzidines; D002863:Chromogenic Compounds; D017895:Manganese Compounds; D010087:Oxides; D014527:Uric Acid; C021758:3,3',5,5'-tetramethylbenzidine; D005978:Glutathione; D008550:Melatonin; D003545:Cysteine; D001205:Ascorbic Acid; C016552:manganese dioxide
keywords: Antioxidants; Discrimination; MnO(2) nanosheets; Sensor array; Visualization