title: [Molecular typing ofSalmonellaisolates from poultry production chains in four cities of Heilongjiang province].
abstract: Objective:To study the PFGE type ofSalmonella(S.) strains isolated from poultry production chains (hatching, breeding, slaughter, distribution and retail) of four cities in Heilongjiang province.Methods:DNA collected fromS. strains in 2012 was digested byXbaⅠ according to the standard PFGE protocol of US CDC. The PFGE patterns were then analyzed by BioNumerics software.Results:The contamination ofS. appeared most serious during the process of slaughtering (13.84%). PFGE was used to determine the genetic relationships between these isolates from poultry production chains, 89 pulsotypes from 150S. enteritidisisolates and 55 pulsotypes from 65S. indianaisolates showed considerable diversity. The same pulsotypes ofS. enteritidiscan be found between different food chains and cities. In contrast, no identical pulsotypes ofS. indianawere found between different food chain and cities. In these four cities, the above said two kinds ofS.were from different sources. The source ofS.contamination in HLJ2 city had been traced back to the chain of poultry hatching.Conclusions:The distribution of pulsetypes of theS. enteritidisandS. indianaisolates was from different regions and the dominant bands were also different between the chains of poultry production. Cross contamination existed in slaughterhouses and contamination can be traced back to the poultry hatching.
authors.affiliation: Heilongjiang Center for Disease Control and Prevention, Harbin 150030, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Yu
authors.affiliation: Heilongjiang Center for Disease Control and Prevention, Harbin 150030, China.
authors.forename: X
authors.initials: X
authors.lastname: Liang
authors.affiliation: China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: L
authors.initials: L
authors.lastname: Bai
authors.affiliation: China National Center for Food Safety Risk Assessment, Beijing 100022, China.
authors.forename: W W
authors.initials: WW
authors.lastname: Li
authors.affiliation: Heilongjiang Center for Disease Control and Prevention, Harbin 150030, China.
authors.forename: J
authors.initials: J
authors.lastname: Yan
authors.affiliation: Heilongjiang Center for Disease Control and Prevention, Harbin 150030, China.
authors.forename: K L
authors.initials: KL
authors.lastname: Wang
authors.affiliation: Heilongjiang Center for Disease Control and Prevention, Harbin 150030, China.
authors.forename: X
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D016521:Electrophoresis, Gel, Pulsed-Field; D058889:Molecular Typing; D011200:Poultry; D011201:Poultry Diseases; D011202:Poultry Products; D012480:Salmonella Infections