title: Long-Range Contacts in Unfolding of Two-State Proteins.
abstract: Predicting the unfolding rates of proteins remains complicated due to the intricacy present in the unfolding pathway of proteins and further it was observed that the experimental unfolding data were less while compared to folding kinetics. The aim of our present work is to show the variation in long-range contacts observed in various sequence separation bins belonging to all-α, all-β and mixed structural classes of 52 two-state proteins. In this work linear regression technique have been used and regression equations were developed using long-range contacts observed from various sequence separation bins. Also nine topological parameters developed from the 3-D structures of proteins are related with their experimental unfolding rates and their variation in correlation coefficient is observed before and after structural classification. The present work aims to show that long-range contacts formed between residues which are sequentially far and spatially close in the 3-D structure of proteins play a crucial role in the unfolding mechanism of proteins. Also importance of long-range contacts in various experimental and theoretical studies of protein folding along with NMR studies of the unfolded non-native states of proteins have been discussed.
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Samuel
authors.affiliation: School of Chemistry, University of Hyderabad, Prof CR Rao Road, Gachibowli, Hyderabad, Telangana 500 046, India.
authors.forename: Harihar
authors.initials: H
authors.lastname: Balasubramanian
mesh_terms: D007700:Kinetics; D016014:Linear Models; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D011489:Protein Denaturation; D017510:Protein Folding; D054730:Protein Interaction Domains and Motifs; D058767:Protein Unfolding; D011506:Proteins / Q000737:chemistry*; D013816:Thermodynamics
chemical_list: D011506:Proteins
keywords: Folding and unfolding rates; bin interval; long-range contacts; sequence separation; structural class