title: Effect of pulsed electric fields (PEF) on accumulation of selenium and zinc ions in Saccharomyces cerevisiae cells.
abstract: The cultures of Saccharomyces cerevisiae were treated with pulsed electric fields (PEF) in order to obtain a maximum accumulation of selenium and zinc ions (simultaneously) in the biomass. The following concentrations: 100μgSe/ml and 150μgZn/ml medium were assumed to be optimal for the maximum accumulation of these ions, that is 43.07mg/gd.m. for selenium and 14.48mg/gd.m. for zinc, in the cultures treated with PEF. At optimal PEF parameters: electric field strength of 3kV/cm and pulse width of 10μs after the treatment of 20-h culture for 10min, the maximum accumulation of both ions in the yeast cells was observed. Application of PEF caused the increase of ions accumulation by 65% for selenium and 100% for zinc. Optimization of PEF parameters led to the further rise in the both ions accumulation resulting in over 2-fold and 2.5-fold higher concentration of selenium and zinc.
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland. Electronic address: urszula.pankiewicz@up.lublin.pl.
authors.forename: Urszula
authors.initials: U
authors.lastname: Pankiewicz
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Sujka
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
authors.forename: Radosław
authors.initials: R
authors.lastname: Kowalski
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
authors.forename: Artur
authors.initials: A
authors.lastname: Mazurek
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Włodarczyk-Stasiak
authors.affiliation: Department of Analysis and Evaluation of Food Quality, Faculty of Food Science and Biotechnology, University of Life Sciences, Skromna 8, 20-704 Lublin, Poland.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Jamroz
mesh_terms: D004560:Electricity; D012441:Saccharomyces cerevisiae / Q000166:cytology / Q000378:metabolism*; D012643:Selenium / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D012643:Selenium; D015032:Zinc
keywords: Bioaccumulation; Electroporation; TEM-EDS; Yeast