title: Hed1 Promotes Meiotic Crossover Formation inSaccharomyces cerevisiae.
abstract: Homologous recombination occurs between homologous chromosomes and is significantly involved in programmed double-strand break (DSB) repair. Activation of two recombinases, Rad51 and Dmc1, is essential for an interhomolog bias during meiosis. Rad51 participates in both mitotic and meiotic recombination, and its strand exchange activity is regulated by an inhibitory factor during meiosis. Thus, activities of Rad51 and Dmc1 are coordinated to promote homolog bias. It has been reported that Hed1, a meiosis-specific protein in budding yeast, regulates Rad51-dependent recombination activity. Here, we investigated the role of Hed1 in meiotic recombination by ectopic expression of the protein after pre-meiotic replication inSaccharomyces cerevisiae. DNA physical analysis revealed that the overexpression of Hed1 delays the DSB-to-joint molecule (JM) transition and promotes interhomolog JM formation. The study indicates a possible role of Hed1 in controlling the strand exchange activity of Rad51 and, eventually, meiotic crossover formation.
authors.affiliation: Department of Life Sciences, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Yoon-Ju
authors.initials: YJ
authors.lastname: Kong
authors.affiliation: Department of Life Sciences, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Jeong-Hwan
authors.initials: JH
authors.lastname: Joo
authors.affiliation: Department of Life Sciences, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Keun Pil
authors.initials: KP
authors.lastname: Kim
authors.affiliation: Department of Life Sciences, Chung-Ang University, Seoul 06974, Republic of Korea.
authors.forename: Soogil
authors.initials: S
authors.lastname: Hong
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D004260:DNA Repair; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism; D059765:Homologous Recombination; D008540:Meiosis / Q000235:genetics; D051135:Rad51 Recombinase / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D018797:Cell Cycle Proteins; C513033:DMC1 protein, S cerevisiae; D004268:DNA-Binding Proteins; C000616116:Hed1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D051135:Rad51 Recombinase
keywords: Hed1; Saccharomyces cerevisiae; meiosis; recombination