title: On-Line Monitoring the Growth of E. coli or HeLa Cells Using an Annular Microelectrode Piezoelectric Biosensor.
abstract: Biological information is obtained from the interaction between the series detection electrode and the organism or the physical field of biological cultures in the non-mass responsive piezoelectric biosensor. Therefore, electric parameter of the electrode will affect the biosensor signal. The electric field distribution of the microelectrode used in this study was simulated using the COMSOL Multiphysics analytical tool. This process showed that the electric field spatial distribution is affected by the width of the electrode finger or the space between the electrodes. In addition, the characteristic response of the piezoelectric sensor constructed serially with an annular microelectrode was tested and applied for the continuous detection ofEscherichia coliculture or HeLa cell culture. Results indicated that the piezoelectric biosensor with an annular microelectrode meets the requirements for the real-time detection ofE. colior HeLa cells in culture. Moreover, this kind of piezoelectric biosensor is more sensitive than the sensor with an interdigital microelectrode. Thus, the piezoelectric biosensor acts as an effective analysis tool for acquiring online cell or microbial culture information.
authors.affiliation: School of Biological and Chemical Engineering, Nanyang Institute of Technology, Nanyang 473000, China. tfeifei99@hotmail.com.
authors.forename: Feifei
authors.initials: F
authors.lastname: Tong
authors.affiliation: School of Chemistry and Materials Engineering, Huaihua University, Huaihua 418000, China. arline7419@hotmail.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lian
authors.affiliation: School of Mechanical and Automotive Engineering, Nanyang Institute of Technology, Nanyang 473000, China. hanhanhgd@163.com.
authors.forename: Junliang
authors.initials: J
authors.lastname: Han
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D004926:Escherichia coli / Q000254:growth & development*; D006367:HeLa Cells; D006801:Humans; D020407:Internet; D008839:Microelectrodes
keywords: annular microelectrodes; biosensor; piezoelectric frequency