title: BKI1 Regulates Plant Architecture through Coordinated Inhibition of the Brassinosteroid and ERECTA Signaling Pathways in Arabidopsis.
abstract: Hundreds of leucine-rich repeat receptor-like kinases (LRR-RLKs) play indispensable roles in a wide range of plant developmental and physiological processes. The mechanisms controlling LRR-RLKs at a basal and inactive status are essential but rarely studied. BKI1 is the only reported inhibitor of receptor kinases in Arabidopsis, which negatively regulates BRI1 in the brassinosteroid pathway. In this study, we found that BKI1 can also interact with another important LRR-RLK, ERECTA (ER). Phenotypic analysis showed that BKI1 and ER together regulate plant architecture, including pedicel orientation, which is a newly reported phenotype in the BR- and ER-mediated developmental processes. Gene expression analysis revealed that BKI1 regulates a subset of ER-responsive genes. Kinase assays demonstrated that BKI1 inhibits ER kinase activity. In addition, the release of BKI1 inhibition on ER signaling relies largely on BRI1 activation. Our data provide significant insights into the regulation and activation of RLKs and suggest that BKI1 functions as a common suppressor of the BRI1 and ER signaling pathways.
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Cangjing
authors.initials: C
authors.lastname: Yang
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, No 1 Shizishan Street, Wuhan, Hubei 430070, China.
authors.forename: Haijiao
authors.initials: H
authors.lastname: Wang
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, No 1 Shizishan Street, Wuhan, Hubei 430070, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering and Institute of Biodiversity Sciences, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Zhuona
authors.initials: Z
authors.lastname: He
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering and Institute of Biodiversity Sciences, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chang
authors.affiliation: State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; Ministry of Education Key Laboratory of Biodiversity Sciences and Ecological Engineering and Institute of Biodiversity Sciences, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200438, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ma
authors.affiliation: College of Life Science and Technology, Huazhong Agricultural University, No 1 Shizishan Street, Wuhan, Hubei 430070, China. Electronic address: xlwang@mail.hzau.edu.cn.
authors.forename: Xuelu
authors.initials: X
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D060406:Brassinosteroids / Q000378:metabolism*; D047428:Protein Kinase Inhibitors / Q000378:metabolism*; D011494:Protein Kinases / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000378:metabolism*; D011956:Receptors, Cell Surface / Q000378:metabolism*; D015398:Signal Transduction
chemical_list: D029681:Arabidopsis Proteins; C513107:BKI1 protein, Arabidopsis; D060406:Brassinosteroids; D047428:Protein Kinase Inhibitors; D011956:Receptors, Cell Surface; D011494:Protein Kinases; C457763:ER protein, Arabidopsis; C108270:BRI1 protein, Arabidopsis; D017346:Protein Serine-Threonine Kinases
keywords: BKI1; ERECTA; crosstalk; kinase inhibitor; pedicel orientation; receptor-like kinase