title: MN1 and PTEN gene expression in acute myeloid leukemia.
abstract: OBJECTIVE
  Multiple genetic alterations with prognostic significance have been discovered in acute myeloid leukemia (AML). We studied the expression level of two genes, Meningioma1 (MN1) and Phosphatase and Tensin homolog (PTEN) to determine their expression in AML patients and their role as prognostic markers.
  
  
  METHODS
  The study included 50 cytogenetic normal de novo AML cases and 10 controls, Their level was detected by Real time Reverse Transcription-Polymerase Chain Reaction.
  
  
  RESULTS
  Relative mRNA expression of MN1 was significantly higher (p value < 0.001) and PTEN expression was significantly lower (p value = 0.002). No correlation was found between neither MN1 nor PTEN mRNA expression and overall survival (p value = 0.212 and 0.310) respectively.
  
  
  CONCLUSIONS
  Although our study suggests a role for MN1 gene and PTEN genes in AML, we could not recommend their use as routine diagnostic and prognostic markers for AML in Egyptian population.
authors.affiliation: Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Giza, Egypt.
authors.forename: Rania A
authors.initials: RA
authors.lastname: Zayed
authors.affiliation: Clinical and Chemical Pathology Department, National Cancer Institute, Cairo University, Giza, Egypt.
authors.forename: Maha A
authors.initials: MA
authors.lastname: Eltaweel
authors.affiliation: Clinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Giza, Egypt.
authors.forename: Shahira K A
authors.initials: SK
authors.lastname: Botros
authors.affiliation: Clinical and Chemical Pathology Department, National Cancer Institute, Cairo University, Giza, Egypt.
authors.forename: Mohamed A
authors.initials: MA
authors.lastname: Zaki
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D016022:Case-Control Studies; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015470:Leukemia, Myeloid, Acute / Q000235:genetics* / Q000401:mortality; D008297:Male; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D015534:Trans-Activators; D025521:Tumor Suppressor Proteins / Q000235:genetics*; D055815:Young Adult
chemical_list: C506524:MN1 protein, human; D015534:Trans-Activators; D025521:Tumor Suppressor Proteins; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: AML; MN1; PCR; PROGNOSIS; PTEN