title: Selenium-Binding Protein 1 (SBP1) autoantibodies in ovarian disorders and ovarian cancer.
abstract: Infertility is a risk factor for ovarian cancer (OvCa). The goal was to determine if antibodies to selenium-binding protein 1 (SBP1), an autoantibody we identified in patients with premature ovarian failure (POF), occurs in both infertility and OvCa patients, and thus could be associated with preneoplasia. Anti-SBP1 was measured by immunoassay against recombinant SBP1, in sera from OvCa (n = 41), infertility (n = 92) and control (n = 87) patients. Infertility causes were POF, unexplained, irregular ovulation or endometriosis. The percent of anti-SBP1-positive sera was higher in POF (P = 0.02), irregular ovulation (P = 0.001), unexplained causes (P = 0.02), late (III-IV)-stage OvCa (P = 0.02) but was not significant in endometriosis, benign ovarian tumors/cysts, early stage (I-II) OvCa or uterine cancer compared to healthy controls. Anti-SBP1 was significantly higher in women with serous (P = 0.04) but not non-serous (P = 0.33) OvCa compared to controls. Also, we determined if anti-SBP1 was associated with CA125 or anti-TP53, markers often studied in OvCa. Anti-TP53 and CA125 were measured by established immunoassays. The ability of anti-SBP1 alone to discriminate infertility or OvCa from controls or when combined with anti-TP53 and CA125, to identify OvCa was evaluated by comparing the area under the curve (AUC) in ROC analysis. Anti-SBP1 alone discriminated infertility (AUC = 0.7; P = 0.001) or OvCa (AUC = 0.67; P = 0.03) from controls. The sensitivity and specificity of OvCa identification was increased by combining CA125, anti-TP53 and anti-SBP1 (AUC = 0.96). Therefore, anti-SBP1 occurs in infertile women with POF, ovulatory disturbances or unexplained infertility and in serous OvCa. This suggests an autoimmune process is associated with the development of serous OvCa.
authors.affiliation: Department of PharmacologyRush University Medical Center, Chicago, Illinois, USA.
authors.forename: Yi
authors.initials: Y
authors.lastname: Yu-Rice
authors.affiliation: Department of PharmacologyRush University Medical Center, Chicago, Illinois, USA.
authors.forename: Seby L
authors.initials: SL
authors.lastname: Edassery
authors.affiliation: Fred Hutchinson Cancer Research CenterSeattle, Washington, USA.
authors.forename: Nicole
authors.initials: N
authors.lastname: Urban
authors.affiliation: Department of PathologyHarborview Medical Center, University of Washington, Seattle, Washington, USA.
authors.forename: Ingegerd
authors.initials: I
authors.lastname: Hellstrom
authors.affiliation: Department of PathologyHarborview Medical Center, University of Washington, Seattle, Washington, USA.
authors.forename: Karl Erik
authors.initials: KE
authors.lastname: Hellstrom
authors.affiliation: Department of Bioinformatics and BiostatisticsRush University Medical Center, Chicago, Illinois, USA.
authors.forename: Youping
authors.initials: Y
authors.lastname: Deng
authors.affiliation: Department of Bioinformatics and BiostatisticsRush University Medical Center, Chicago, Illinois, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of PharmacologyRush University Medical Center, Chicago, Illinois, USA JLuborsky@whoi.edu.
authors.forename: Judith L
authors.initials: JL
authors.lastname: Luborsky
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood* / Q000276:immunology; D014408:Biomarkers, Tumor / Q000097:blood*; D016022:Case-Control Studies; D018284:Cystadenocarcinoma, Serous / Q000097:blood / Q000276:immunology*; D004715:Endometriosis / Q000097:blood / Q000276:immunology*; D005260:Female; D006801:Humans; D007247:Infertility, Female / Q000097:blood / Q000276:immunology*; D008875:Middle Aged; D010051:Ovarian Neoplasms / Q000097:blood / Q000276:immunology*; D016649:Primary Ovarian Insufficiency / Q000097:blood / Q000276:immunology*; D012372:ROC Curve; D051137:Selenium-Binding Proteins / Q000276:immunology*; D055815:Young Adult
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; C495175:SELENBP1 protein, human; D051137:Selenium-Binding Proteins