title: Metabolic Engineering ofPseudomonas putidaKT2440 for the Production ofpara-Hydroxy Benzoic Acid.
abstract: para-Hydroxy benzoic acid (PHBA) is the key component for preparing parabens, a common preservatives in food, drugs, and personal care products, as well as high-performance bioplastics such as liquid crystal polymers.Pseudomonas putidaKT2440 was engineered to produce PHBA from glucoseviathe shikimate pathway intermediate chorismate. To obtain the PHBA production strain, chorismate lyase UbiC fromEscherichia coliand a feedback resistant 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase encoded by genearoGD146Nwere overexpressed individually and simultaneously. In addition, genes related to product degradation (pobA) or competing for the precursor chorismate (pheAandtrpE) were deleted from the genome. To further improve PHBA production, the glucose metabolism repressorhexRwas knocked out in order to increase erythrose 4-phosphate and NADPH supply. The best strain achieved a maximum titer of 1.73 g L-1and a carbon yield of 18.1% (C-mol C-mol-1) in a non-optimized fed-batch fermentation. This is to date the highest PHBA concentration produced byP. putidausing a chorismate lyase.
authors.affiliation: Centre for Microbial Electrochemical Systems (CEMES), The University of Queensland, Brisbane, QLD, Australia; Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Shiqin
authors.initials: S
authors.lastname: Yu
authors.affiliation: Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD, Australia; Metabolomics Australia (Queensland Node), The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Manuel R
authors.initials: MR
authors.lastname: Plan
authors.affiliation: Centre for Microbial Electrochemical Systems (CEMES), The University of Queensland, Brisbane, QLD, Australia; Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane, QLD, Australia; School of Science and Technology, The University of New England, Armidale, NSW, Australia.
authors.forename: Gal
authors.initials: G
authors.lastname: Winter
authors.affiliation: Centre for Microbial Electrochemical Systems (CEMES), The University of Queensland, Brisbane, QLD, Australia; Advanced Water Management Centre (AWMC), The University of Queensland, Brisbane, QLD, Australia.
authors.forename: Jens O
authors.initials: JO
authors.lastname: Krömer
keywords: Pseudomonas putida KT2440; metabolic engineering; para-hydroxy benzoic acid; shikimate pathway