title: A sensitive aptasensor based on molybdenum carbide nanotubes and label-free aptamer for detection of bisphenol A.
abstract: To specifically and sensitively identify bisphenol A (BPA) with a simple and rapid method is very important for food safety. Using an anti-BPA aptamer and Mo2C nanotubes, we developed a label-free and low-background signal biosensor for BPA detection. The anti-BPA aptamer drastically increased the fluorescence signal of N-methylmesoporphyrin IX under an assistance of Help-DNA. Additionally, BPA can interact with the anti-BPA aptamer and switch its conformation to prevent the formation of a G-quadruplex, resulting in fluorescence quenching. Simultaneously, Mo2C nanotubes can reduce the background signals due to the adsorption of Help-DNA on their surface. This method shows a linear range of 2-20 nM with a detection limit of 2 nM for detecting BPA. This label-free BPA aptasensor with low background signal is inexpensive, easy to use, and can be applied to determine BPA in real water samples. Graphical Abstract A low-background and label-free biosensor was designed based on Mo2C nanotubes and aptamer for BPA detection.
authors.affiliation: Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China.
authors.forename: Meng-Qi
authors.initials: MQ
authors.lastname: He
authors.affiliation: Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China. yuyl@mail.neu.edu.cn.
authors.forename: Yong-Liang
authors.initials: YL
authors.lastname: Yu
authors.affiliation: Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, China. herh@mail.neu.edu.cn.
authors.forename: Rong-Huan
authors.initials: RH
authors.lastname: He
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis*; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008982:Molybdenum / Q000737:chemistry*; D043942:Nanotubes / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D008982:Molybdenum; D010636:Phenols; C574181:molybdenum carbide; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Aptamer; Biosensor; Bisphenol A; Label-free; Mo2C nanotubes