title: Lectin from seeds of a Brazilian lima bean variety (Phaseolus lunatus L. var. cascavel) presents antioxidant, antitumour and gastroprotective activities.
abstract: Lectins are proteins able to interact specifically and reversibly with carbohydrates. They are present in all living beings, particularly in legume seeds, which have many biological functions. The aim of this study was to isolate, characterize and verify antioxidant, anti-hemolytic, antitumor and gastroprotective activities in a lectin present in seeds of Phaseolus lunatus L. var. cascavel (PLUN). The isolation of lectin was performed by size exclusion chromatography on Sephadex G-100, which was isolated from a protein capable of agglutinating only human erythrocytes type A, being this the only inhibited haemagglutination n-acetyl-d-galactosamine. Its weight was estimated by PAGE is 128kDa. The lectin is thermostable up to 80°C and is active between pH 2-11. As 8M urea was able to denature the lectin. PLUN is a glycoprotein consisting of 2% carbohydrate and has antioxidant action with ascorbic acid equivalent antioxidant capacity (μMAA/g) of 418.20, 326 and 82.9 for total antioxidant activity, ABTS radical capture and capture of DPPH radical, respectively. The lectin has antitumor activity against melanoma derived cells at doses of 100 and 50mg/ml, reducing up to 83% tumor cells, and gastroprotective action, reducing up to 63% damaged area of ​​the stomach induced by ethanol.
authors.affiliation: Master by the Graduate Programme in Cellular and Molecular Biology at the Federal University of Paraíba (Universidade Federal da Paraíba), João Pessoa, Brazil. Electronic address: rodrigofarmapb@gmail.com.
authors.forename: Rodrigo Rodrigues
authors.initials: RR
authors.lastname: E Lacerda
authors.affiliation: Federal University of Paraíba (Universidade Federal da Paraíba), João Pessoa, Brazil. Electronic address: edilsa_nat@hotmail.com.
authors.forename: Edilza Silva
authors.initials: ES
authors.lastname: do Nascimento
authors.affiliation: Federal University of Paraíba (Universidade Federal da Paraíba), João Pessoa, Brazil. Electronic address: thalles_lacerda2@hotmail.com.
authors.forename: José Thalles Jocelino Gomes
authors.initials: JT
authors.lastname: de Lacerda
authors.affiliation: Federal University of Pelotas (Universidade Federal de Pelotas), Pelotas, Brazil. Electronic address: ls_pinto@hotmail.com.
authors.forename: Luciano da Silva
authors.initials: LD
authors.lastname: Pinto
authors.affiliation: Federal University of Pelotas (Universidade Federal de Pelotas), Pelotas, Brazil. Electronic address: ccrizzi@yahoo.com.br.
authors.forename: Caroline
authors.initials: C
authors.lastname: Rizzi
authors.affiliation: Federal University of Ceará (Universidade Federal do Ceará), Sobral, Brazil. Electronic address: mirnabrayner@gmail.com.
authors.forename: Mirna Marques
authors.initials: MM
authors.lastname: Bezerra
authors.affiliation: Federal University of Ceará (Universidade Federal do Ceará), Sobral, Brazil. Electronic address: isabelaribeirosb@gmail.com.
authors.forename: Isabela Ribeiro
authors.initials: IR
authors.lastname: Pinto
authors.affiliation: Federal University of Ceará (Universidade Federal do Ceará), Sobral, Brazil. Electronic address: samuelmateus.ufc@hotmail.com.
authors.forename: Samuel Mateus Pereira
authors.initials: SM
authors.lastname: Filho
authors.affiliation: Federal University of Ceará (Universidade Federal do Ceará), Sobral, Brazil. Electronic address: pintovicente@gmail.com.
authors.forename: Vicente de Paulo Texeira
authors.initials: VP
authors.lastname: Pinto
authors.affiliation: Federal University of Ceará (Universidade Federal do Ceará), Sobral, Brazil. Electronic address: gerardocristino@uol.com.br.
authors.forename: Gerardo Cristino
authors.initials: GC
authors.lastname: Filho
authors.affiliation: Federal University of Paraíba (Universidade Federal da Paraíba), João Pessoa, Brazil. Electronic address: calbgadelha@gmail.com.
authors.forename: Carlos Alberto de Almeida
authors.initials: CA
authors.lastname: Gadelha
authors.affiliation: Federal University of Paraíba (Universidade Federal da Paraíba), João Pessoa, Brazil. Electronic address: santi.tatiane@gmail.com.
authors.forename: Tatiane Santi
authors.initials: TS
authors.lastname: Gadelha
mesh_terms: D000116:Acetylgalactosamine / Q000737:chemistry; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D052160:Benzothiazoles / Q000037:antagonists & inhibitors / Q000737:chemistry; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors / Q000737:chemistry; D045744:Cell Line, Tumor; D004912:Erythrocytes / Q000187:drug effects; D000431:Ethanol; D005765:Gastrointestinal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006386:Hemagglutination Tests; D006801:Humans; D008297:Male; D051379:Mice; D008970:Molecular Weight; D027805:Phaseolus / Q000737:chemistry*; D010851:Picrates / Q000037:antagonists & inhibitors / Q000737:chemistry; D037121:Plant Lectins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D011489:Protein Denaturation; D012639:Seeds / Q000737:chemistry; D052616:Solid Phase Extraction / Q000379:methods; D013270:Stomach / Q000187:drug effects / Q000473:pathology; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D013451:Sulfonic Acids / Q000037:antagonists & inhibitors / Q000737:chemistry; D014508:Urea / Q000737:chemistry
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D000975:Antioxidants; D052160:Benzothiazoles; D001713:Biphenyl Compounds; D005765:Gastrointestinal Agents; D010851:Picrates; D037121:Plant Lectins; D013451:Sulfonic Acids; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D000431:Ethanol; D014508:Urea; C004931:1,1-diphenyl-2-picrylhydrazyl; D000116:Acetylgalactosamine
keywords: Antioxidant; Antitumour; Gastroprotective; Lectin; Phaseolus; Purification