title: Identification of MSX1 and DCLK1 as mRNA Biomarkers for Colorectal Cancer Detection Through DNA Methylation Information.
abstract: Colorectal cancer is the second most deadly malignancy in the United States. However, the currently screening options had their limitation. Novel biomarkers for colorectal cancer detections are necessary to reduce the mortality. The clinical information, mRNA expression levels and DNA methylation information of colorectal cancer were downloaded from TCGA. The patients were separated into training group and testing group based on their platforms for DNA methylation. Beta values of DNA methylation from tumor tissues and normal tissues were utilized to figure out the position that were differentially methylated. The expression levels of mRNA of thirteen genes, whose CpG islands were differentially methylated, were extracted from the RNA-Seq results from TCGA. The probabilities whether the mRNA was differentially expressed between tumor and normal samples were calculated using Student's t-test. Logistic regression and decision tree were built for cancer detection and their performances were evaluated by the area under the curve (AUC). Twenty-four genomic locations were differentially methylated, which could be mapped to eleven genes. Nine out of eleven genes had differentially expressed mRNA levels, which were used to build the model for cancer detection. The final detection models consisting of mRNA expression levels of these nine genes had great performances on both training group and testing group. The model that constructed in this study suggested MSX1 and DCLK1 might be used in colorectal cancer detection or as target of cancer therapies. J. Cell. Physiol. 232: 1879-1884, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of General Surgery, The Affiliated Huai'an Hospital of Xuzhou Medical University and The Second People's Hospital of Huai'an, Huai'an, China.
authors.forename: Ai-Jun
authors.initials: AJ
authors.lastname: Sun
authors.affiliation: Department of General Surgery, Huai'an Tumor Hospital, Huai'an, China.
authors.forename: Hai-Bo
authors.initials: HB
authors.lastname: Gao
authors.affiliation: Department of Gastrointestinal Surgery, Enshi Clinical College of Wuhan University, Central Hospital of Enshi Autonomous Prefecture, Enshi, Hubei, China.
authors.forename: Gao
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Intestinal Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Heng-Fa
authors.initials: HF
authors.lastname: Ge
authors.affiliation: Department of Cardiology, The Fifth People's Hospital of Shanghai, Fudan University, Shanghai, China.
authors.forename: Zun-Ping
authors.initials: ZP
authors.lastname: Ke
authors.affiliation: Department of Spinal Surgery, Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics*; D018899:CpG Islands / Q000235:genetics; D019175:DNA Methylation / Q000235:genetics*; D003663:Decision Trees; D000089742:Doublecortin-Like Kinases; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000235:genetics* / Q000378:metabolism; D016015:Logistic Models; D051957:MSX1 Transcription Factor / Q000235:genetics* / Q000378:metabolism; D017346:Protein Serine-Threonine Kinases / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012372:ROC Curve
chemical_list: D014408:Biomarkers, Tumor; D047908:Intracellular Signaling Peptides and Proteins; D051957:MSX1 Transcription Factor; C484083:MSX1 protein, human; D012333:RNA, Messenger; C117347:DCLK1 protein, human; D000089742:Doublecortin-Like Kinases; D017346:Protein Serine-Threonine Kinases