title: 1D NMR methods for determination of degree of cross-linking and BDDE substitution positions in HA hydrogels.
abstract: Hyaluronic acid polymers cross-linked with BDDE are today among the most used hydrogels for biomedical applications. The physical properties of the hydrogels depend, among other parameters, on the degree of cross-linking of HA. Another parameter likely to affect the physical properties is the substitution position of the linker on the HA functional groups. A NMR-based method for the determination of these parameters in hyaluronic acid hydrogels is presented. The method is based on the degradation of HA cross linked hydrogels by chondroitinase ABC followed by one-dimensional1H and13C NMR analysis. The necessary structural information to obtain both the degree of cross-linking and the substitution positions can be obtained from the same NMR sample and no chromatographic separation step is required prior to NMR analysis.
authors.affiliation: Department of Chemistry and Biotechnology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden. Electronic address: Frida.Wende@slu.se.
authors.forename: Frida J
authors.initials: FJ
authors.lastname: Wende
authors.affiliation: Department of Chemistry and Biotechnology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden. Electronic address: Suresh.Gohil@slu.se.
authors.forename: Suresh
authors.initials: S
authors.lastname: Gohil
authors.affiliation: Research A&C Galderma, Seminariegatan 21, SE-752 28 Uppsala, Sweden. Electronic address: Lars.Nord@galderma.com.
authors.forename: Lars I
authors.initials: LI
authors.lastname: Nord
authors.affiliation: Research A&C Galderma, Seminariegatan 21, SE-752 28 Uppsala, Sweden. Electronic address: Anne.HelanderKenne@galderma.com.
authors.forename: Anne
authors.initials: A
authors.lastname: Helander Kenne
authors.affiliation: Department of Chemistry and Biotechnology, Uppsala BioCenter, Swedish University of Agricultural Sciences, P.O. Box 7015, SE-750 07 Uppsala, Sweden. Electronic address: Corine.sandstrom@slu.se.
authors.forename: Corine
authors.initials: C
authors.lastname: Sandström
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D006820:Hyaluronic Acid / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy
chemical_list: D020100:Hydrogels; D006820:Hyaluronic Acid
keywords: Cross-linking ratio; Hyaluronic acid; Hydrogels; NMR spectroscopy; Substitution position