title: Determination of neurotoxic agents as markers of common vetch adulteration in lentil by LC-MS/MS.
abstract: Vicia sativa (common vetch), which contains β-cyanoalanine (BCA) and γ-glutamyl β-cyanoalanine (γGBCA) is used for adulteration of lentil and have neuro toxic effects on people and animals. Therefore the determination of BCA and γGBCA analytes in accurate and reliable manner has high importance for human and animal health. LC-IDMS/MS method has been developed and validated for quantitative analyses of β-cyanoalanine and13C labeled BCA is used as an internal standard in γGBCA analysis. In order to validate the method, linearity, recovery, precision (repeatability), intermediate precision, limit of detection, and limit of quantification parameters were investigated. The correlation coefficient was found to be greater than 0.99 for both analytes. The recoveriess were determined as 95.8% for BCA and 97.4% for γGBCA. The relative expanded measurement uncertainties of β-cyanoalanine and γ-glutamyl-β-cyanoalanine were obtained as 4.6% and 5.8%, respectively with the coverage factor, k, is 2 and at 95% confidence level.
authors.affiliation: Department of Chemistry, Faculty of Sciences, Ataturk University, 25240 Erzurum, Turkey.
authors.forename: Nesa
authors.initials: N
authors.lastname: Ghasemi
authors.affiliation: Department of Chemistry, Faculty of Sciences, Ataturk University, 25240 Erzurum, Turkey.
authors.forename: Hasan
authors.initials: H
authors.lastname: Secen
authors.affiliation: TUBITAK UME (National Metrology Institute), Chemistry Group, Organic Chemistry Laboratories, P.O. Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Hasibe
authors.initials: H
authors.lastname: Yılmaz
authors.affiliation: TUBITAK UME (National Metrology Institute), Chemistry Group, Organic Chemistry Laboratories, P.O. Box 54, 41470 Gebze-Kocaeli, Turkey.
authors.forename: Burcu
authors.initials: B
authors.lastname: Binici
authors.affiliation: TUBITAK UME (National Metrology Institute), Chemistry Group, Organic Chemistry Laboratories, P.O. Box 54, 41470 Gebze-Kocaeli, Turkey. Electronic address: ahmetceyhan.goren@tubitak.gov.tr.
authors.forename: Ahmet C
authors.initials: AC
authors.lastname: Goren
mesh_terms: D000409:Alanine / Q000031:analogs & derivatives* / Q000032:analysis; D002853:Chromatography, Liquid / Q000379:methods; D006801:Humans; D027806:Lens Plant / Q000737:chemistry*; D053719:Tandem Mass Spectrometry / Q000379:methods; D033022:Vicia sativa / Q000737:chemistry*
chemical_list: C004631:3-cyanoalanine; D000409:Alanine
keywords: Common vetch; LC-MS/MS; Lentil; Method validation; Uncertainty; β-Cyano-l-alanine