title: Animal models in genomic research: Techniques, applications, and roles for nurses.
abstract: Animal research has been conducted by scientists for over two millennia resulting in a better understanding of human anatomy, physiology, and pathology, as well as testing of novel therapies. In the molecular genomic era, pre-clinical models represent a key tool for understanding the genomic underpinnings of health and disease and are relevant to precision medicine initiatives. Nurses contribute to improved health by collecting and translating evidence from clinically relevant pre-clinical models. Using animal models, nurses can ask questions that would not be feasible or ethical to address in humans, and establish the safety and efficacy of interventions before translating them to clinical trials. Two advantages of using pre-clinical models are reduced variability between test subjects and the opportunity for precisely controlled experimental exposures. Standardized care controls the effects of diet and environment, while the availability of inbred strains significantly reduces the confounding effects of genetic differences. Outside the laboratory, nurses can contribute to the approval and oversight of animal studies, as well as translation to clinical trials and, ultimately, patient care. This review is intended as a primer on the use of animal models to advance nursing science; specifically, the paper discusses the utility of preclinical models for studying the pathophysiologic and genomic contributors to health and disease, testing interventions, and evaluating effects of environmental exposures. Considerations specifically geared to nurse researchers are also introduced, including discussion of how to choose an appropriate model and controls, potential confounders, as well as legal and ethical concerns. Finally, roles for nurse clinicians in pre-clinical research are also highlighted.
authors.affiliation: University of Pittsburgh School of Nursing, Pittsburgh, PA 15213; Safar Center for Resuscitation Research, Pittsburgh, PA 15213.
authors.forename: Nicole D
authors.initials: ND
authors.lastname: Osier
authors.affiliation: University of Pittsburgh School of Nursing, Pittsburgh, PA 15213.
authors.forename: Lan
authors.initials: L
authors.lastname: Pham
authors.affiliation: University of Pittsburgh School of Nursing, Pittsburgh, PA 15213.
authors.forename: Amanda
authors.initials: A
authors.lastname: Savarese
authors.affiliation: University of Pittsburgh School of Nursing, Pittsburgh, PA 15213; McGowan Institute for Regenerative Medicine, Pittsburgh, PA, 15260.
authors.forename: Kendra
authors.initials: K
authors.lastname: Sayles
authors.affiliation: University of Pittsburgh School of Nursing, Pittsburgh, PA 15213; University of Pittsburgh School of Medicine, Pittsburgh, PA 15213. Electronic address: salexand@pitt.edu.
authors.forename: Sheila A
authors.initials: SA
authors.lastname: Alexander
mesh_terms: D000818:Animals; D023281:Genomics; D023421:Models, Animal; D024802:Nurse's Role; D012106:Research
keywords: Animal models; Genetics; Genomics; Nursing science; Pre-clinical; Transgenic