title: The membrane localization domains of two distinct bacterial toxins form a 4-helix-bundle in solution.
abstract: Membrane localization domain (MLD) was first proposed for a 4-helix-bundle motif in the crystal structure of the C1 domain of Pasteurella multocida toxin (PMT). This structure motif is also found in the crystal structures of several clostridial glycosylating toxins (TcdA, TcdB, TcsL, and TcnA). The Ras/Rap1-specific endopeptidase (RRSP) module of the multifunctional autoprocessing repeats-in-toxins (MARTX) toxin produced by Vibrio vulnificus has sequence homology to the C1-C2 domains of PMT, including a putative MLD. We have determined the solution structure for the MLDs in PMT and in RRSP using solution state NMR. We conclude that the MLDs in these two toxins assume a 4-helix-bundle structure in solution.
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Illinois.
authors.forename: Grant S
authors.initials: GS
authors.lastname: Hisao
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Illinois.
authors.forename: Michael C
authors.initials: MC
authors.lastname: Brothers
authors.affiliation: Department of Microbiology, University of Illinois at Urbana-Champaign, Illinois.
authors.forename: Mengfei
authors.initials: M
authors.lastname: Ho
authors.affiliation: Department of Microbiology, University of Illinois at Urbana-Champaign, Illinois.
authors.forename: Brenda A
authors.initials: BA
authors.lastname: Wilson
authors.affiliation: Department of Chemistry, University of Illinois at Urbana-Champaign, Illinois.|Department of Biochemistry, University of Illinois at Urbana-Champaign, Illinois.|Center for Biophysics and Quantitative Biology, University of Illinois at Urbana-Champaign, Illinois.
authors.forename: Chad M
authors.initials: CM
authors.lastname: Rienstra
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001427:Bacterial Toxins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D002462:Cell Membrane / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D016979:Pasteurella multocida / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D000072417:Protein Domains; D017433:Protein Structure, Secondary; D017386:Sequence Homology, Amino Acid; D041261:Vibrio vulnificus / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; C062258:Pasteurella multocida toxin
keywords: 4-helix-bundle; Pasteurella multocida; Vibrio vulnificus; bacterial toxin; membrane localization domains; solution NMR spectroscopy