title: Evaluation of an enzyme immunoassay for the detection of the mycotoxin tenuazonic acid in sorghum grains and sorghum-based infant food.
abstract: An enzyme-linked immunosorbent assay (ELISA) for the Alternaria mycotoxin tenuazonic acid (TeA) was evaluated by comparative analysis of naturally contaminated sorghum grains and sorghum-based infant food, using a stable isotope dilution LC-MS assay (SIDA; limit of detection (LOD) 1.0 μg/kg) as the reference method. LODs of the ELISA were 30 μg/kg in sorghum grains and 220 μg/kg in sorghum-based infant cereals. With SIDA, 100% of the samples (n = 28) had been positive for TeA in a concentration range of 6-584 μg/kg (mean 113 μg/kg). The ELISA consistently detected TeA in all naturally contaminated samples at cut-off levels of 30-60 μg/kg (sorghum) and 200-300 μg/kg (infant cereals), as based on corresponding to SIDA values. Although the ELISA was much less sensitive than the SIDA method, it may be useful as a screening method for sorghum and sorghum-based infant foods and can be employed to identify samples containing elevated concentrations of TeA in food, well below the proposed level of concern (500 μg/kg).
authors.affiliation: Veterinary Food Diagnostics, Institute of Veterinary Food Science, Veterinary Faculty, Justus Liebig-University, Ludwigstr. 21, 35390, Giessen, Germany. madeleine.gross@vetmed.uni-giessen.de.
authors.forename: Madeleine
authors.initials: M
authors.lastname: Gross
authors.affiliation: Analytical Food Chemistry, Technische Universität München, 85354, Freising, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Asam
authors.affiliation: Analytical Food Chemistry, Technische Universität München, 85354, Freising, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Rychlik
mesh_terms: D000903:Antibiotics, Antineoplastic; D002853:Chromatography, Liquid; D059022:Food Safety / Q000379:methods*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D007225:Infant Food / Q000032:analysis*; D008403:Mass Screening / Q000379:methods; D013058:Mass Spectrometry; D009183:Mycotoxins / Q000032:analysis*; D012680:Sensitivity and Specificity; D045868:Sorghum / Q000737:chemistry*; D013720:Tenuazonic Acid / Q000032:analysis*
chemical_list: D000903:Antibiotics, Antineoplastic; D009183:Mycotoxins; D013720:Tenuazonic Acid
keywords: Enzyme immunoassay; Infant food; Mycotoxin; Stable isotope dilution LC-MS assay; Tenuazonic acid