title: An ELISPOT-Based Assay to Measure HBV-Specific CD8+T Cell Responses in Immunocompetent Mice.
abstract: Despite some important limitations, immunocompetent mouse models of HBV replication remain an essential tool for studying cellular and humoral immunity to the virus. CD8+T cells are a critical component of the immune response to HBV due to their ability to both kill virus-infected hepatocytes and produce cytokines such as IFN-γ that non-cytopathically inhibit virus replication. A number of techniques can be used to measure the magnitude, specificity, and functionality of HBV-specific CD8+T cells, each having its own unique advantages. We describe here the enzyme-linked immunospot (ELISPOT)-based assay, which, compared to other methods, is sensitive, cost-effective, and rapid and requires relatively little optimization, specialized training, or equipment.
authors.affiliation: Department of Pathology, Yale University School of Medicine, New Haven, CT, USA.
authors.forename: Tracy D
authors.initials: TD
authors.lastname: Reynolds
authors.affiliation: Department of Immunology and Microbial Disease, Albany Medical College, Albany, NY, USA.
authors.forename: Safiehkhatoon
authors.initials: S
authors.lastname: Moshkani
authors.affiliation: Department of Immunology and Microbial Disease, Albany Medical College, Albany, NY, USA. robekm@mail.amc.edu.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Robek
mesh_terms: D000818:Animals; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology* / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D058501:Enzyme-Linked Immunospot Assay; D018984:Epitopes, T-Lymphocyte / Q000276:immunology; D006509:Hepatitis B / Q000276:immunology* / Q000378:metabolism / Q000821:virology; D006515:Hepatitis B virus / Q000276:immunology*; D051379:Mice; D037182:T-Cell Antigen Receptor Specificity / Q000276:immunology*
chemical_list: D016207:Cytokines; D018984:Epitopes, T-Lymphocyte
keywords: ELISPOT; HBV mouse models; IFN-γ; T cell