title: Nonequilibrium description of de novo biogenesis and transport through Golgi-like cisternae.
abstract: A central issue in cell biology is the physico-chemical basis of organelle biogenesis in intracellular trafficking pathways, its most impressive manifestation being the biogenesis of Golgi cisternae. At a basic level, such morphologically and chemically distinct compartments should arise from an interplay between the molecular transport and chemical maturation. Here, we formulate analytically tractable, minimalist models, that incorporate this interplay between transport and chemical progression in physical space, and explore the conditions for de novo biogenesis of distinct cisternae. We propose new quantitative measures that can discriminate between the various models of transport in a qualitative manner-this includes measures of the dynamics in steady state and the dynamical response to perturbations of the kind amenable to live-cell imaging.
authors.affiliation: Department of Theoretical Physics, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai, 400005, India.|Institute of Science and Technology, Am Campus 1, Klosterneuburg, A-3400, Austria.
authors.forename: Himani
authors.initials: H
authors.lastname: Sachdeva
authors.affiliation: Department of Theoretical Physics, Tata Institute of Fundamental Research, Homi Bhabha Road, Mumbai, 400005, India.|TIFR Centre for Interdisciplinary Sciences, 21 Brundavan Colony, Narsingi, Hyderabad 500075, India.
authors.forename: Mustansir
authors.initials: M
authors.lastname: Barma
authors.affiliation: Simons Centre for the Study of Living Machines, National Centre for Biological Sciences (TIFR), Bellary Road, Bangalore, 560065, India.
authors.forename: Madan
authors.initials: M
authors.lastname: Rao
mesh_terms: D001693:Biological Transport, Active / Q000502:physiology; D006056:Golgi Apparatus / Q000378:metabolism*; D008954:Models, Biological