title: Global profiling of viral and cellular non-coding RNAs in Epstein-Barr virus-induced lymphoblastoid cell lines and released exosome cargos.
abstract: The human EBV-transformed lymphoblastoid cell line (LCL), obtained by infecting peripheral blood monocular cells with Epstein-Barr Virus, has been extensively used for human genetic, pharmacogenomic, and immunologic studies. Recently, the role of exosomes has also been indicated as crucial in the crosstalk between EBV and the host microenvironment. Because the role that the LCL and LCL exosomal cargo might play in maintaining persistent infection, and since little is known regarding the non-coding RNAs of LCL, the aim of our work was the comprehensive characterization of this class of RNA, cellular and viral miRNAs, and cellular lncRNAs, in LCL compared with PBMC derived from the same donors. In this study, we have demonstrated, for the first time, that all the viral miRNAs expressed by LCL are also packaged in the exosomes, and we found that two miRNAs, ebv-miR-BART3 and ebv-miR-BHRF1-1, are more abundant in the exosomes, suggesting a microvescicular viral microRNA transfer. In addition, lncRNA profiling revealed that LCLs were enriched in lncRNA H19 and H19 antisense, and released these through exosomes, suggesting a leading role in the regulation of the tumor microenvironment.
authors.affiliation: Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad alta specializzazione), Italy. Electronic address: agallo@ismett.edu.
authors.forename: Alessia
authors.initials: A
authors.lastname: Gallo
authors.affiliation: Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad alta specializzazione), Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Vella
authors.affiliation: Fondazione Ri.MED, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Miele
authors.affiliation: Fondazione Ri.MED, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Timoneri
authors.affiliation: Fondazione Ri.MED, Italy.
authors.forename: Mariangela
authors.initials: M
authors.lastname: Di Bella
authors.affiliation: Fondazione Ri.MED, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Bosi
authors.affiliation: Pediatric Hepatology and Liver Transplantation, IRCCS ISMETT, University of Pittsburgh Medical Center, Palermo, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Sciveres
authors.affiliation: Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad alta specializzazione), Italy; Fondazione Ri.MED, Italy.
authors.forename: Pier Giulio
authors.initials: PG
authors.lastname: Conaldi
mesh_terms: D045744:Cell Line, Tumor; D020031:Epstein-Barr Virus Infections / Q000235:genetics* / Q000473:pathology; D055354:Exosomes / Q000378:metabolism; D006801:Humans; D008223:Lymphoma / Q000821:virology*; D062085:RNA, Long Noncoding / Q000235:genetics*
chemical_list: D062085:RNA, Long Noncoding
keywords: Epstein–Barr virus; Exosomes; Lymphoblastoid cell lines; lncRNA; miRNA