title: Digital PCR dynamic range is approaching that of real-time quantitative PCR.
abstract: Digital PCR (dPCR) has been reported to be more precise and sensitive than real-time quantitative PCR (qPCR) in a variety of models and applications. However, in the majority of commercially available dPCR platforms, the dynamic range is dependent on the number of partitions analysed and so is typically limited to four orders of magnitude; reduced compared with the typical seven orders achievable by qPCR. Using two different biological models (HIV DNA analysis andKRASgenotyping), we have demonstrated that the RainDrop Digital PCR System (RainDance Technologies) is capable of performing accurate and precise quantification over six orders of magnitude thereby approaching that achievable by qPCR.
authors.affiliation: Molecular and Cell Biology Team, LGC, Teddington, United Kingdom.
authors.forename: Gerwyn M
authors.initials: GM
authors.lastname: Jones
authors.affiliation: Molecular and Cell Biology Team, LGC, Teddington, United Kingdom.
authors.forename: Eloise
authors.initials: E
authors.lastname: Busby
authors.affiliation: Department of Infection, Division of Infection and Immunity, University College London, London, UK.
authors.forename: Jeremy A
authors.initials: JA
authors.lastname: Garson
authors.affiliation: Virology Laboratory, Clinical Microbiology and Virology, University College London Hospital NHS Foundation Trust, London, United Kingdom.
authors.forename: Paul R
authors.initials: PR
authors.lastname: Grant
authors.affiliation: Virology Laboratory, Clinical Microbiology and Virology, University College London Hospital NHS Foundation Trust, London, United Kingdom.
authors.forename: Eleni
authors.initials: E
authors.lastname: Nastouli
authors.affiliation: Molecular and Cell Biology Team, LGC, Teddington, United Kingdom.
authors.forename: Alison S
authors.initials: AS
authors.lastname: Devonshire
authors.affiliation: Molecular and Cell Biology Team, LGC, Teddington, United Kingdom.
authors.forename: Alexandra S
authors.initials: AS
authors.lastname: Whale