title: Profiling of Selected Functional Metabolites in the Central Nervous System of Marine Medaka (Oryzias melastigma) for Environmental Neurotoxicological Assessments.
abstract: The simultaneous profiling of 43 functional metabolites in the brain of the small model vertebrate organism, marine medaka (Oryzais melastigma), has been accomplished via dansyl chloride derivatization and LC-MS/MS quantification. This technique was applied to examine effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47), one of the most abundant polybrominated diphenyl ether flame retardants in the natural environment, on the central nervous system (CNS) of vertebrates. The model teleosts were fed with bioencapsulated Artemia nauplii for up to 21 days. Multivariate statistical analysis has demonstrated that levels of numerous classical neurotransmitters and their metabolites in the CNS of the fish were perturbed even at the early phase of dietary exposure. Subsequent metabolic pathway analysis further implied potential impairment of the arginine and proline metabolism; glycine, serine and threonine metabolism; D-glutamine and D-glutamate metabolism; alanine, aspartate, and glutamate metabolism; valine, leucine, and isoleucine biosynthesis, and the cysteine and methionine metabolism in the brain of the test organism. Our results demonstrate that targeted profiling of functional metabolites in the CNS may shed light on how the various neurological pathways of vertebrates, including humans, are affected by toxicant/stress exposure.
authors.affiliation: State Key Laboratory for Marine Pollution, Department of Biology & Chemistry, City University of Hong Kong, Tat Chee Ave., Kowloon, Hong Kong SAR, China.|State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong SAR, China.
authors.forename: Elva Ngai-Yu
authors.initials: EN
authors.lastname: Lei
authors.affiliation: State Key Laboratory for Marine Pollution, Department of Biology & Chemistry, City University of Hong Kong, Tat Chee Ave., Kowloon, Hong Kong SAR, China.
authors.forename: Man-Shan
authors.initials: MS
authors.lastname: Yau
authors.affiliation: State Key Laboratory for Marine Pollution, Department of Biology & Chemistry, City University of Hong Kong, Tat Chee Ave., Kowloon, Hong Kong SAR, China.
authors.forename: Chi-Chung
authors.initials: CC
authors.lastname: Yeung
authors.affiliation: State Key Laboratory for Marine Pollution, Department of Biology & Chemistry, City University of Hong Kong, Tat Chee Ave., Kowloon, Hong Kong SAR, China.
authors.forename: Margaret B
authors.initials: MB
authors.lastname: Murphy
authors.affiliation: State Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Hong Kong SAR, China.
authors.forename: Ka-Leung
authors.initials: KL
authors.lastname: Wong
authors.affiliation: State Key Laboratory for Marine Pollution, Department of Biology & Chemistry, City University of Hong Kong, Tat Chee Ave., Kowloon, Hong Kong SAR, China. bhmhwlam@cityu.edu.hk.
authors.forename: Michael Hon-Wah
authors.initials: MH
authors.lastname: Lam
mesh_terms: D000818:Animals; D002490:Central Nervous System / Q000187:drug effects; D002853:Chromatography, Liquid / Q000662:veterinary; D003619:Dansyl Compounds / Q000737:chemistry; D005411:Flame Retardants / Q000633:toxicity; D055768:Halogenated Diphenyl Ethers / Q000633:toxicity*; D018377:Neurotransmitter Agents / Q000378:metabolism*; D009990:Oryzias / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000662:veterinary; D018675:Toxicity Tests / Q000379:methods / Q000662:veterinary*; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D003619:Dansyl Compounds; D005411:Flame Retardants; D055768:Halogenated Diphenyl Ethers; D018377:Neurotransmitter Agents; D014874:Water Pollutants, Chemical; C511295:2,2',4,4'-tetrabromodiphenyl ether; C029038:dansyl chloride