title: Influence of Animal Heating on PET Imaging Quantification and Kinetics: Biodistribution of18F-Tetrafluoroborate and18F-FDG in Mice.
abstract: Different environmental conditions under anesthesia may lead to unstable homeostatic conditions in rodents and therefore may alter kinetics. In this study, the impact of different heating conditions on PET imaging quantification was evaluated.Methods:Two groups of 6 adult female BALB/c nude mice with subcutaneously implanted tumors underwent microPET imaging after injection of18F-labeled tetrafluoroborate or18F-FDG. Dynamic scans were acquired under optimal and suboptimal heating conditions. Time-activity curves were analyzed to calculate uptake and washout time constants.Results:With18F-labeled tetrafluoroborate, optimal animal heating led to a stable heart rate during acquisition (515 ± 35 [mean ± SD] beats/min), whereas suboptimal heating led to a lower heart rate and a higher SD (470 ± 84 beats/min). Both uptake and washout time constants were faster (P< 0.01) in animals maintained with optimal heating.Conclusion:Although the difference in heart rates was slight, optimal heating yielded significantly faster uptake and washout kinetics than suboptimal heating in all organs for both tracers.
authors.affiliation: UF6237 Imagerie Préclinique, Pôle d'Imagerie, CHU Hautepierre, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.|ICube, Département Mécanique, CNRS, Strasbourg, France.|Department of Nuclear Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany; and.
authors.forename: Christian
authors.initials: C
authors.lastname: Goetz
authors.affiliation: Department of Nuclear Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany; and.
authors.forename: Matthias
authors.initials: M
authors.lastname: Podein
authors.affiliation: Department of Nuclear Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany; and.
authors.forename: Friederike
authors.initials: F
authors.lastname: Braun
authors.affiliation: Molecular Imaging and Therapy Service, Memorial Sloan Kettering Cancer Center, New York, New York.
authors.forename: Wolfgang A
authors.initials: WA
authors.lastname: Weber
authors.affiliation: UF6237 Imagerie Préclinique, Pôle d'Imagerie, CHU Hautepierre, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.|ICube, Département Mécanique, CNRS, Strasbourg, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Choquet
authors.affiliation: UF6237 Imagerie Préclinique, Pôle d'Imagerie, CHU Hautepierre, Hôpitaux Universitaires de Strasbourg, Strasbourg, France aconstant@unistra.fr.|ICube, Département Mécanique, CNRS, Strasbourg, France.
authors.forename: André
authors.initials: A
authors.lastname: Constantinesco
authors.affiliation: Department of Nuclear Medicine, Medical Center-University of Freiburg, Faculty of Medicine, University of Freiburg, Freiburg, Germany; and.
authors.forename: Michael
authors.initials: M
authors.lastname: Mix
mesh_terms: D000818:Animals; D001831:Body Temperature; D001881:Borates; D001888:Boric Acids / Q000493:pharmacokinetics*; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000493:pharmacokinetics*; D006339:Heart Rate; D006361:Heating; D008657:Metabolic Clearance Rate; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism*; D009928:Organ Specificity; D049268:Positron-Emission Tomography / Q000379:methods*; D019275:Radiopharmaceuticals / Q000493:pharmacokinetics; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D014018:Tissue Distribution
chemical_list: D001881:Borates; D001888:Boric Acids; D019275:Radiopharmaceuticals; D019788:Fluorodeoxyglucose F18; C098759:fluoroboric acid
keywords: PET kinetics; homeostasis; tracer biodistribution; xenografts