title: Metabolic engineering of fatty alcohol production in transgenic hairy roots of Crambe abyssinica.
abstract: Biotechnological production of fatty alcohols, important raw materials in the chemical industry, has been receiving considerable attention in recent years. Fatty alcohols are formed by the reduction of fatty acyl-CoAs or fatty acyl-ACPs catalyzed by a fatty acyl reductase (FAR). In this study, we introduced genes encoding FARs from Arabidopsis thaliana (AtFAR5) and Simmondsia chinensis (ScFAR) into Crambe abyssinica hairy roots via Agrobacterium rhizogenes-mediated transformation. The efficiency of the transformation ranged between 30 and 45%. The fatty alcohols were only detected in the transgenic hairy root lines expressing ScFAR gene. In all tested lines stearyl alcohol (18:0-OH), arachidyl alcohol (20:0-OH), and behenyl alcohol (22:0-OH) were produced. The content of 18:0-OH varied from 1 to 3% of total fatty acids and fatty alcohols, while the amount of either 20:0-OH and 22:0-OH did not exceed 2%. The transgenic hairy root lines produced from 0.98 to 2.59 nmol of fatty alcohols per mg of dry weight. Very low activity of ScFAR was detected in the microsomal fractions isolated from the selected hairy root lines. To our knowledge, this is the first report on the fatty alcohol production in the hairy root cultures. Biotechnol. Bioeng. 2017;114: 1275-1282. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Plant Physiology and Biotechnology, University of Gdańsk, Wita Stwosza 59, 80-308, Gdańsk, Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Miklaszewska
authors.affiliation: Laboratory of Plant Biochemistry, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdańsk, Abrahama, Gdańsk, Poland.
authors.forename: Antoni
authors.initials: A
authors.lastname: Banaś
authors.affiliation: Laboratory of Biologically Active Compounds, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdańsk, Abrahama, Gdańsk, Poland.
authors.forename: Aleksandra
authors.initials: A
authors.lastname: Królicka
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000235:genetics* / Q000378:metabolism*; D031218:Crambe Plant / Q000502:physiology*; D005233:Fatty Alcohols / Q000302:isolation & purification / Q000378:metabolism*; D060847:Metabolic Engineering / Q000379:methods*; D018517:Plant Roots / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism
chemical_list: D005233:Fatty Alcohols; D011994:Recombinant Proteins; D000445:Aldehyde Oxidoreductases
keywords: Crambe abyssinica; Simmondsia chinensis; fatty acyl reductase; fatty alcohols; hairy roots