title: Antiproliferative Activity and Apoptosis Inducing Mechanism ofAnthocephalus cadambaon Dalton's Lymphoma Ascites Cells.
abstract: The purpose of this investigation was to evaluate the antiproliferative and apoptogenic mechanistic studies of methanol extract ofAnthocephalus cadamba(MEAC) on Dalton's lymphoma ascites (DLA) cells treated mice. Determination of antiproliferative activity was performed by using different DLA cells (2×106cells, i.p.) inoculated mice groups (n= 12). Groups were treated for 14 consecutive days with MEAC at the doses of 200 and 400 mg/Kg b.w. respectively. The mechanism of antiproliferation activity of MEAC was investigated through morphological studies by acridine orange (AO)/ethidium bromide (EB) double staining method. Comet assay was estimated to check the DNA damage induced apoptosis property. Furthermore, flow cytometry (FACS) was used to quantitatively detect the apoptotic rate by double labeling techniques using Annexin-V FITC/propidium iodide staining analysis and apoptotic proteins expression done by western blotting assay method. MEAC exhibited significant (p<0.01) decrease the tumor volume, viable cell count, tumor weight and elevated the life span of DLA tumor bearing mice. Analysis of AO/EB staining and flow cytometry showed that MEAC possessed apoptosis induced antitumor activity on DLA cells in a dose dependant manner. Dose dependent induction of DNA damage on DLA cells were observed after MEAC treatment, which was evident from the appearance of comet tail length. Pro-apoptotic gene, Bax was up-regulated and down-regulation of the Bcl-2/Bax ratio, suggesting that Bcl-2 family involved in the control of apoptosis. Experimental results revealed that MEAC possess potent antitumor activityviainduction of cancer cell apoptosis mechanism.
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
authors.forename: Narayan
authors.initials: N
authors.lastname: Dolai
authors.affiliation: Research and Development Centre, Natreon Inc, Salt Lake City, Kolkata, India.
authors.forename: Aminul
authors.initials: A
authors.lastname: Islam
authors.affiliation: Department of Pharmaceutical Technology, Jadavpur University, Kolkata, India.
authors.forename: Pallab Kanti
authors.initials: PK
authors.lastname: Haldar
keywords: Anthocephalus cadamba; Antitumor; Apoptosis; DLA