title: [Role of galectin-1 in regulation of umbilical cord mesenchymal stem cells on T cells of rheumatoid arthritis].
abstract: The therapeutic potential of umbilical cord mesenchymal stem cells (UC-MSCs) in rheumatoid arthritis (RA) has attracted more and more attention, because of it can suppress the various inflammatory effects of T cells. Galectin-1 is highly expressed in UC-MSCs, as the first lectin mediating the immunomodulatory effect of MSCs. Our study will investigate the effects of galectin-1 in regulation of UC-MSCs on rheumatoid arthritis T cells.
  
  
  
  Lentivirus transfected shRNA technique was used to knock down the expression of galectin-1 in UC-MSCs to construct UC-MSCs(Gal-1-). The effects of UC-MSCs and UC-MSCs(Gal-1-) on CD4+T cells in RA patients were investigated by contact system, including negative control group (CD4+T cells), positive control group [CD4+T-phytohemagg lutinin (PHA)], UC-MSCs-CD4+T cells co-culture group, UC-MSCs(control shRNA)-CD4+T cells co-culture group, and UC-MSCs(Gal-1-)-CD4+T cells co-culture group. The proliferation of CD4+T cells was detected by MTS assay. The level of tumor necrosis factors α (TNF-α) in cells supernatant was detected by enzyme linked immunosorbent assay (ELISA). The effect of UC-MSCs on helper T cell (Th) subset was detected by flow cytometry.
  
  
  
  In vitro, UC-MSCs were capable of inhibiting PHA induced proliferation of CD4+T cells from RA patients, but UC-MSCs(Gal-1-) did not show the significant inhibitory effect. Galectin-1 affect the TNF-α level of CD4+T cells regulated by UC-MSCs. UC-MSCs and UC-MSCs(control shRNA) significantly inhibited the expression of TNF-α in PHA-induced CD4+T cells. However, UC-MSCs(Gal-1-) had no significant inhibitory effect. Furthermore, the Th1 cells were also significantly suppressed by UC-MSCs and UC-MSCs(control shRNA) (4.83%±1.37% and 5.13%±0.87%,P=0.012 and P=0.018). These was no significant difference in the proportion of the Th1 cells between the control group and UC-MSCs(Gal-1-) group (8.51%±2.04% and 6.41%±0.96%,P=0.101). The Th2 cells were protected after silence galectin-1 in UC-MSCs, whereas there was no significant difference. The proportion of Th17 was decreased by co-culture with UC-MSCs and UC-MSCs (control shRNA), but these was also no significant difference.
  
  
  
  UC-MSCs can inhibit the proliferation and differentiation of CD4+T cells from RA patients, but these effect declined after knocking down the expression of galectin-1. Galectin-1 maybe take part in the regulation of UC-MSCs on rheumatoid arthritis CD4+T cells.
authors.affiliation: Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: L
authors.initials: L
authors.lastname: Sun
authors.affiliation: Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: Z
authors.initials: Z
authors.lastname: An
authors.affiliation: Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: C H
authors.initials: CH
authors.lastname: Li
authors.affiliation: Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: R
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: J X
authors.initials: JX
authors.lastname: Zhao
authors.affiliation: Department of Rheumatology and Immunology, Peking University Third Hospital, Beijing 100191, China.
authors.forename: X Y
authors.initials: XY
authors.lastname: Liu
mesh_terms: D001172:Arthritis, Rheumatoid / Q000276:immunology* / Q000628:therapy*; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D002454:Cell Differentiation / Q000187:drug effects / Q000276:immunology; D049109:Cell Proliferation / Q000187:drug effects; D018920:Coculture Techniques; D036101:Cord Blood Stem Cell Transplantation; D005434:Flow Cytometry; D037483:Galectin 1 / Q000276:immunology* / Q000627:therapeutic use; D006801:Humans; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000276:immunology; D058504:Th17 Cells; D014409:Tumor Necrosis Factor-alpha / Q000276:immunology
chemical_list: D037483:Galectin 1; D014409:Tumor Necrosis Factor-alpha