title: Survivin Improves Reprogramming Efficiency of Human Neural Progenitors by Single Molecule OCT4.
abstract: Induced pluripotent stem (iPS) cells have been generated from human somatic cells by ectopic expression of four Yamanaka factors. Here, we report that Survivin, an apoptosis inhibitor, can enhance iPS cells generation from human neural progenitor cells (NPCs) together with one factor OCT4 (1F-OCT4-Survivin). Compared with 1F-OCT4, Survivin accelerates the process of reprogramming from human NPCs. The neurocyte-originated induced pluripotent stem (NiPS) cells generated from 1F-OCT4-Survivin resemble human embryonic stem (hES) cells in morphology, surface markers, global gene expression profiling, and epigenetic status. Survivin keeps high expression in both iPS and ES cells. During the process of NiPS cell to neural cell differentiation, the expression of Survivin is rapidly decreased in protein level. The mechanism of Survivin promotion of reprogramming efficiency from NPCs may be associated with stabilization ofβ-catenin in WNT signaling pathway. This hypothesis is supported by experiments of RT-PCR, chromatin immune-precipitation, and Western blot in human ES cells. Our results showed overexpression of Survivin could improve the efficiency of reprogramming from NPCs to iPS cells by one factor OCT4 through stabilization of the key molecule,β-catenin.
authors.affiliation: Department of Cell Biology and Stem Cell Research Center, School of Basic Medicine, Peking University, Beijing, China.
authors.forename: Shixin
authors.identifier: 0000-0002-1192-7616
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Cell Biology and Stem Cell Research Center, School of Basic Medicine, Peking University, Beijing, China.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Baotou Medical College, Baotou, Inner Mongolia, China.
authors.forename: Ruopeng
authors.initials: R
authors.lastname: Feng
authors.affiliation: Beijing Cellapy Biotechnology Co., LTD, Beijing, China.
authors.forename: Caiyun
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutics, University of Florida, 6550 Sanger Road, Orlando, FL 32827, USA.
authors.forename: Sibo
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Department of Cell Biology and Stem Cell Research Center, School of Basic Medicine, Peking University, Beijing, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Beijing Anzhen Hospital, Beijing, China.
authors.forename: Feng
authors.identifier: 0000-0002-2629-601X
authors.initials: F
authors.lastname: Lan
authors.affiliation: Department of Cell Biology and Stem Cell Research Center, School of Basic Medicine, Peking University, Beijing, China.
authors.forename: Yang
authors.identifier: 0000-0002-9797-6442
authors.initials: Y
authors.lastname: Li