title: The interaction of wood nanocellulose dressings and the wound pathogen P. aeruginosa.
abstract: Chronic wounds pose an increasingly significant worldwide economic burden (over £1 billion per annum in the UK alone). With the escalation in global obesity and diabetes, chronic wounds will increasingly be a significant cause of morbidity and mortality. Cellulose nanofibrils (CNF) are highly versatile and can be tailored with specific physical properties to produce an assortment of three-dimensional structures (hydrogels, aerogels or films), for subsequent utilization as wound dressing materials. Growth curves using CNF (diameter <20nm) in suspension demonstrated an interesting dose-dependent inhibition of bacterial growth. In addition, analysis of biofilm formation (Pseudomonas aeruginosa PAO1) on nanocellulose aerogels (20g/m2) revealed significantly less biofilm biomass with decreasing aerogel porosity and surface roughness. Importantly, virulence factor production by P. aeruginosa in the presence of nanocellulose materials, quantified for the first time, was unaffected (p>0.05) over 24h. These data demonstrate the potential of nanocellulose materials in the development of novel dressings that may afford significant clinical potential.
authors.affiliation: Advanced Therapies Group, Oral and Biomedical Sciences, Cardiff University School of Dentistry, Cardiff CF14 4XY, UK. Electronic address: ally.a.jack@gmail.com.
authors.forename: Alison A
authors.initials: AA
authors.lastname: Jack
authors.affiliation: Department of Cancer Research and Molecular Medicine, NTNU, Trondheim, Norway.
authors.forename: Henriette R
authors.initials: HR
authors.lastname: Nordli
authors.affiliation: Advanced Therapies Group, Oral and Biomedical Sciences, Cardiff University School of Dentistry, Cardiff CF14 4XY, UK.
authors.forename: Lydia C
authors.initials: LC
authors.lastname: Powell
authors.affiliation: Advanced Therapies Group, Oral and Biomedical Sciences, Cardiff University School of Dentistry, Cardiff CF14 4XY, UK.
authors.forename: Kate A
authors.initials: KA
authors.lastname: Powell
authors.affiliation: Advanced Therapies Group, Oral and Biomedical Sciences, Cardiff University School of Dentistry, Cardiff CF14 4XY, UK.
authors.forename: Himanshu
authors.initials: H
authors.lastname: Kishnani
authors.affiliation: PFI, Høgskoleringen 6b, NO-7491 Trondheim, Norway.
authors.forename: Per Olav
authors.initials: PO
authors.lastname: Johnsen
authors.affiliation: Department of Cancer Research and Molecular Medicine, NTNU, Trondheim, Norway; Department of Dermatology, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
authors.forename: Brita
authors.initials: B
authors.lastname: Pukstad
authors.affiliation: Advanced Therapies Group, Oral and Biomedical Sciences, Cardiff University School of Dentistry, Cardiff CF14 4XY, UK.
authors.forename: David W
authors.initials: DW
authors.lastname: Thomas
authors.affiliation: PFI, Høgskoleringen 6b, NO-7491 Trondheim, Norway. Electronic address: gary.chinga.carrasco@pfi.no.
authors.forename: Gary
authors.initials: G
authors.lastname: Chinga-Carrasco
authors.affiliation: Advanced Therapies Group, Oral and Biomedical Sciences, Cardiff University School of Dentistry, Cardiff CF14 4XY, UK.
authors.forename: Katja E
authors.initials: KE
authors.lastname: Hill
mesh_terms: D001458:Bandages; D018441:Biofilms; D002482:Cellulose / Q000737:chemistry*; D053758:Nanoparticles; D011550:Pseudomonas aeruginosa / Q000254:growth & development; D014934:Wood
chemical_list: D002482:Cellulose
keywords: Biofilms; COMSTAT; Characterisation; Nanocellulose; Pseudomonas aeruginosa