title: Curd development associated gene (CDAG1) in cauliflower (Brassica oleracea L. var. botrytis) could result in enlarged organ size and increased biomass.
abstract: The curd is a specialized organ and the most important product organ of cauliflower (Brassica oleracea L. var. botrytis). However, the mechanism underlying the regulation of curd formation and development remains largely unknown. In the present study, a novel homologous gene containing the Organ Size Related (OSR) domain, namely, CDAG1 (Curd Development Associated Gene 1) was identified in cauliflower. Quantitative analysis indicated that CDAG1 showed significantly higher transcript levels in young tissues. Functional analysis demonstrated that the ectopic overexpression of CDAG1 in Arabidopsis and cauliflower could significantly promote organ growth and result in larger organ size and increased biomass. Organ enlargement was predominantly due to increased cell number. In addition, 228 genes involved in the CDAG1-mediated regulatory network were discovered by transcriptome analysis. Among these genes, CDAG1 was confirmed to inhibit the transcriptional expression of the endogenous OSR genes, ARGOS and ARL, while a series of ethylene-responsive transcription factors (ERFs) were found to increased expression in 35S:CDAG1 transgenic Arabidopsis plants. This implies that CDAG1 may function in the ethylene-mediated signal pathway. These findings provide new insight into the function of OSR genes, and suggest potential applications of CDAG1 in breeding high-yielding crops.
authors.affiliation: College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin, 300384, China. Electronic address: lihui@tjau.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: 15822076271@163.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: 13553162779@163.com.
authors.forename: Qingli
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: erjunqin@126.com.
authors.forename: Erjun
authors.initials: E
authors.lastname: Qin
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: emojinchuan@163.com.
authors.forename: Chuan
authors.initials: C
authors.lastname: Jin
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: 1581257798@qq.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: alexmaymolecular@126.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Wu
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: 13752041387@163.com.
authors.forename: Guangshuang
authors.initials: G
authors.lastname: Shen
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: htg1979@163.com.
authors.forename: Chengbin
authors.initials: C
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: songwenqin53@gmail.com.
authors.forename: Wenqin
authors.initials: W
authors.lastname: Song
authors.affiliation: College of Life Sciences, Nankai University, Tianjin, 300071, China. Electronic address: wangcg@nankai.edu.cn.
authors.forename: Chunguo
authors.initials: C
authors.lastname: Wang
mesh_terms: D017360:Arabidopsis / Q000235:genetics; D018533:Biomass; D001937:Brassica / Q000033:anatomy & histology / Q000235:genetics / Q000254:growth & development*; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D010802:Phylogeny; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D030821:Plants, Genetically Modified / Q000254:growth & development; D000072417:Protein Domains; D012333:RNA, Messenger / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism / Q000502:physiology
chemical_list: D010940:Plant Proteins; D012333:RNA, Messenger; D014157:Transcription Factors
keywords: CDAG1; Cauliflower (Brassica oleracea L. var. botrytis); High-yielding crops; OSR gene; Organ size