title: Diverse Profiles of AI-1 Type Quorum Sensing Molecules in Cultivable Bacteria from the Mangrove (Kandelia obovata) Rhizosphere Environment.
abstract: Mangrove rhizosphere environment harbors diverse populations of microbes, and some evidence showed that rhizobacteria behavior was regulated by quorum sensing (QS). Investigating the diverse profiles of QS molecules in mangrove ecosystems may shed light on the bacterial roles and lead to a better understanding of the symbiotic interactions between plants and microbes. The aims of the current study focus on identifying AI-1 type QS signals, i.e., acyl homoserine lactones (AHLs), inKandelia obovatarhizosphere environment. Approximately 1200 rhizobacteria were screened and 184 strains (15.3%) tested were positive. Subsequent 16s rRNA gene sequencing and dereplication analyses identified 24 species from the positive isolates, which were affiliated to three different phyla, including Proteobacteria, Firmicutes, and Actinobacteria. Thin-layer chromatography separation of extracts revealed diverse AHL profiles and detected at least one active compound in the supernatant of these 24 cultivable AHL-producers. The active extracts from these bacterial isolates were further evaluated by ultra performance liquid chromatography-mass spectrometry, and the carbon side chain length ranged from C4 to C14. This is the first report on the diversity of AI-1 type auto-inducers in the mangrove plantK. obovata, and it is imperative to expand our knowledge of plant-bacteria interactions with respect to the maintenance of wetland ecosystem health.
authors.affiliation: The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua UniversityShenzhen, China; Shenzhen Public Platform for Screening and Application of Marine Microbial ResourcesShenzhen, China.
authors.forename: Zhi P
authors.initials: ZP
authors.lastname: Ma
authors.affiliation: The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua UniversityShenzhen, China; Shenzhen Public Platform for Screening and Application of Marine Microbial ResourcesShenzhen, China.
authors.forename: Yong M
authors.initials: YM
authors.lastname: Lao
authors.affiliation: The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua UniversityShenzhen, China; Shenzhen Public Platform for Screening and Application of Marine Microbial ResourcesShenzhen, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Jin
authors.affiliation: The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua University Shenzhen, China.
authors.forename: Guang H
authors.initials: GH
authors.lastname: Lin
authors.affiliation: The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua UniversityShenzhen, China; Shenzhen Public Platform for Screening and Application of Marine Microbial ResourcesShenzhen, China.
authors.forename: Zhong H
authors.initials: ZH
authors.lastname: Cai
authors.affiliation: The Division of Ocean Science and Technology, Graduate School at Shenzhen, Tsinghua UniversityShenzhen, China; Shenzhen Public Platform for Screening and Application of Marine Microbial ResourcesShenzhen, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhou
keywords: acyl homoserine lactone; diverse profiles; mangrove plant; plant-microbes interactions; quorum sensing; rhizobacteria