title: Use of small-angle X-ray scattering to resolve intracellular structure changes ofEscherichia colicells induced by antibiotic treatment.
abstract: The application of small-angle X-ray scattering (SAXS) to wholeEscherichia colicells is challenging owing to the variety of internal constituents. To resolve their contributions, the outer shape was captured by ultra-small-angle X-ray scattering and combined with the internal structure resolved by SAXS. Building on these data, a model for the major structural components ofE. coliwas developed. It was possible to deduce information on the occupied volume, occurrence and average size of the most important intracellular constituents: ribosomes, DNA and proteins.E. coliwas studied after treatment with three different antibiotic agents (chloramphenicol, tetracycline and rifampicin) and the impact on the intracellular constituents was monitored.
authors.affiliation: Analytical Chemistry - Biointerfaces, Ruhr-University Bochum , Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: A R
authors.initials: AR
authors.lastname: von Gundlach
authors.affiliation: Helmholtz-Zentrum Geesthacht, Zentrum für Material- und Küstenforschung GmbH , Max-Planck-Strasse 1, 21502 Geesthacht, Germany.
authors.forename: V M
authors.initials: VM
authors.lastname: Garamus
authors.affiliation: Lawrence Livermore National Laboratory , 7000 East Avenue, Livermore, CA 94550, USA.
authors.forename: T M
authors.initials: TM
authors.lastname: Willey
authors.affiliation: X-ray Science Division, Argonne National Laboratory , 9700 South Cass Avenue, Argonne, IL 60439, USA.
authors.forename: J
authors.initials: J
authors.lastname: Ilavsky
authors.affiliation: Institute of Infection and Immunity, St George's University of London (SGUL) , Cranmer Terrace, London SW17 0RE, UK.
authors.forename: K
authors.initials: K
authors.lastname: Hilpert
authors.affiliation: Analytical Chemistry - Biointerfaces, Ruhr-University Bochum , Universitätsstrasse 150, 44780 Bochum, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Rosenhahn
keywords: Escherichia coli ultrastructure; SAXS; TEM; USAXS; antibiotics; small-angle X-ray scattering; transmission electron microscopy; ultra-small-angle X-ray scattering