title: Expression of CD68 positive macrophages in the use of different barrier materials to prevent peritoneal adhesions-an animal study.
abstract: In preventing postoperative adhesion formation the optimal barrier material has still not been found. It is therefore imperative to assess the biocompatibility of potential barrier devices. Macrophages play a decisive role in the regulation of wound healing, tissue regeneration and foreign body reaction. Since the number of CD68-positive macrophages represents an important parameter within biomaterial testing, in the present study it was analysed whether a correlation exists between the total number of CD68-positive macrophages and the extent of fibrosis or inflammation in peritoneal adhesion prevention using biomaterials. After standardized peritoneal wounding, Wistar rats were treated with five adhesion barriers or remained untreated as a control. After 14 days, animals were sacrificed and the treated areas were evaluated histomorphologically and immunohistologically. A heterogeneous pattern of macrophage count in relation to fibrosis or inflammation was found. While some groups described a moderate macrophage infiltration without fibrosis, others showed similar numbers of macrophages, but accompanied by moderate fibrosis. Moreover, a minimal number of macrophages was associated with minimal fibrosis. Mild inflammation was seen both with minimal and moderate macrophage infiltration. Altogether, no correlation could be established between the tissue response and the count of CD68-positive macrophages. With a view to macrophage heterogeneity further studies are required to determine the different macrophage subpopulations and clarify the role of these in the tissue responses to barrier materials.
authors.affiliation: REPAIR-lab, Institute of Pathology, University of Regensburg, Regensburg, Germany. christoph.brochhausen@ukr.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Brochhausen
authors.affiliation: Cardiology I, Centre for Cardiology, University Medical Centre, Johannes Gutenberg University of Mainz, Mainz, Germany.
authors.forename: Volker H
authors.initials: VH
authors.lastname: Schmitt
authors.affiliation: REPAIR-lab, Institute of Pathology, University of Regensburg, Regensburg, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Mamilos
authors.affiliation: Department of Internal Medicine, St. Vincenz and Elisabeth Hospital of Mainz (KKM), Mainz, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Schmitt
authors.affiliation: Department of Gynaecology and Obstetrics, University of Tuebingen, Tuebingen, Germany.|German Centre of Biomaterials and Artificial Organs e.V. Denkendorf, Denkendorf, Germany.
authors.forename: Constanze N E
authors.initials: CN
authors.lastname: Planck
authors.affiliation: Brigham and Women's Hospital, Harvard Medical School, Boston, USA.
authors.forename: Taufiek K
authors.initials: TK
authors.lastname: Rajab
authors.affiliation: German Centre of Biomaterials and Artificial Organs e.V. Denkendorf, Denkendorf, Germany.|Institute of Textile Technology and Process Engineering, Denkendorf, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: Hierlemann
authors.affiliation: Department of Biomaterials, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
authors.forename: C James
authors.initials: CJ
authors.lastname: Kirkpatrick
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000378:metabolism*; D015214:Antigens, Differentiation, Myelomonocytic / Q000378:metabolism*; D001672:Biocompatible Materials / Q000737:chemistry*; D002448:Cell Adhesion; D005260:Female; D005355:Fibrosis / Q000473:pathology; D005549:Foreign-Body Reaction; D007249:Inflammation; D008264:Macrophages / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D010537:Peritoneum / Q000473:pathology; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D000267:Tissue Adhesions / Q000473:pathology; D014945:Wound Healing / Q000502:physiology; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; D001672:Biocompatible Materials; C539355:CD68 protein, rat; D000097382:CD68 Molecule