title: UHPLC-ESI-MS/MS and GC-MS Analyses on Phenolic, Fatty Acid and Essential Oil ofVerbascum pinetorumwith Antioxidant, Anticholinesterase, Antimicrobial and DNA Damage Protection Effects.
abstract: This paper is the first phytochemical and ABTS cation radical decolorisation activity, cupric reducing antioxidant capacity, anticholinesterase and DNA damage protection effect of endemicVerbascum pinetorum(Boiss.) O. Kuntze. Phenolic profile ofV. pinetorumwere qualified and quantified by UHPLC-ESI-MS/MS analysis. Malic acid (47250.61±2504.28 µg/g) and luteolin (7651.96±527.98 µg/g) were found as most abundant compounds for metanol and acetone extracts, respectively. Fatty acid and essential oil compositions were determined by GC-MS analysis. The main components of fatty acid were found to be palmitic (27.1%) and stearic (22.1%) acids. The main compounds of the essential oil were cineole (16.9%) and α-selinene (16.4%). The acetone extract was found to be more active than BHT used as a standard in β-carotene-linoleic acid test system. In DPPH free radical scavenging activity, the acetone and methanol extracts showed higher activity than BHT at all tested concentrations. The acetone, methanol and water extracts showed strong inhibition while the acetone extract showed better activity than BHT and α-tocopherol which were used as standards in ABTS cation radical scavenging and cupric reducing antioxidant capacity assays, respectively. All extracts were found to be inactive in antialzheimer activity. The acetone extract exhibited moderate antimicrobial activity againstC. albicans.The methanol extract ofV. pinetorumwere found no significant effect on DNA cleavage protection.
authors.affiliation: Department of Pharmaceutical Technology, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Boğa
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Abdulselam
authors.initials: A
authors.lastname: Ertaş
authors.affiliation: Research and Application of Science and Technology Center (DUBTAM), Dicle University, 21280 Diyarbakir, Turkey.
authors.forename: Mustafa Abdullah
authors.initials: MA
authors.lastname: Yılmaz
authors.affiliation: Department of Chemistry, Faculty of Science and Arts, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kızıl
authors.affiliation: Department of Chemistry, Faculty of Science and Arts, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: Bircan
authors.initials: B
authors.lastname: Çeken
authors.affiliation: Department of Nutrition and Dietetics, School of Health, Batman University, 72060 Batman, Turkey.
authors.forename: Nesrin
authors.initials: N
authors.lastname: Haşimi
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Istanbul University, 34116 Istanbul, Turkey.
authors.forename: Tuğba Yılmaz
authors.initials: TY
authors.lastname: Özden
authors.affiliation: Department of Pharmaceutical Botany, Faculty of Pharmacy, Cukurova University, 01330 Balcali, Adana, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Demirci
authors.affiliation: Department of Analytical Chemistry, Faculty of Pharmacy, Dicle University, 21280 Diyarbakır, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Yener
authors.affiliation: Department of Infectious diseases and Clinical Microbiology, Faculty of Medicine, Dicle University, 21280, Diyarbakır, Turkey.
authors.forename: Özcan
authors.initials: Ö
authors.lastname: Deveci
keywords: DNA cleavage protection; Verbascum pinetorum; anticholinesterase; antimicrobial; antioxidant; essential oil; fatty acid