title: Antibiofilm and Anti-β-Lactamase Activities of Burdock Root Extract and Chlorogenic Acid againstKlebsiella pneumoniae.
abstract: Small phytochemicals have been successfully adopted as antibacterial chemotherapies and are being increasingly viewed as potential antibiofilm agents. Some of these molecules are known to repress biofilm and toxin production by certain bacterial and yeast pathogens, but information is lacking with regard to the genes allied with biofilm formation. The present study was performed to investigate the inhibitory effect of burdock root extract (BRE) and of chlorogenic acid (CGA; a component of BRE) on clinical isolates ofKlebsiella pneumoniae. BRE and CGA exhibited significant antibiofilm activity againstK. pneumoniaewithout inflicting any harm to its planktonic counterparts. In vitro assays supported the β-lactamase inhibitory effect of CGA and BRE while in silico docking showed that CGA bound strongly with the active sites of sulfhydryl-variable-1 β-lactamase. Furthermore, the mRNA transcript levels of two biofilm-associated genes (type 3 fimbriaemrkDand trehalose-6-phosphate hydrolasetreC) were significantly downregulated in CGA- and BRE-treated samples. In addition, CGA inhibited biofilm formation byEscherichia coliandCandida albicanswithout affecting their planktonic cell growth. These findings show that BRE and its component CGA have potential use in antibiofilm strategies against persistentK. pneumoniaeinfections.
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan, 38541, Republic of Korea.|Centre for Research and Development, PRIST University, Thanjavur 614-904, Tamil Nadu, India.
authors.forename: Satish Kumar
authors.initials: SK
authors.lastname: Rajasekharan
authors.affiliation: Centre for Research and Development, PRIST University, Thanjavur 614-904, Tamil Nadu, India.
authors.forename: Samiraj
authors.initials: S
authors.lastname: Ramesh
authors.affiliation: Department of Biotechnology, Holy Cross College, Tiruchirappalli 620-020, Tamil Nadu, India.
authors.forename: Ann Susan
authors.initials: AS
authors.lastname: Satish
authors.affiliation: School of Chemical Engineering, Yeungnam University, Gyeongsan, 38541, Republic of Korea.
authors.forename: Jintae
authors.initials: J
authors.lastname: Lee
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D031170:Arctium / Q000737:chemistry*; D001665:Binding Sites; D018441:Biofilms / Q000187:drug effects*; D002726:Chlorogenic Acid / Q000737:chemistry / Q000494:pharmacology*; D006860:Hydrogen Bonding; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000502:physiology*; D008826:Microbial Sensitivity Tests; D008968:Molecular Conformation; D062105:Molecular Docking Simulation; D056004:Molecular Dynamics Simulation; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D018517:Plant Roots / Q000737:chemistry*; D011485:Protein Binding; D053038:Quorum Sensing / Q000187:drug effects; D065093:beta-Lactamase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D001618:beta-Lactamases / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D065093:beta-Lactamase Inhibitors; D002726:Chlorogenic Acid; D001618:beta-Lactamases
keywords: Burdock root; In silico docking; K. pneumoniae; biofilms; chlorogenic acid; β-lactamase