title: [The MicroRNA miR-205 inhibits epithelial-messenchymal transition in HK-2 cells by down-regulating ZEB1 and ZEB2 expressions].
abstract: OBJECTIVE
  To explore the role of miR-205 in regulating epithelial-messenchymal transition (EMT) in proximal tubular cell line HK-2 cells and the underlying mechanism.
  
  
  METHODS
  HK-2 cells transfected with miR-205 mimics or a scrambled control sequence were examined for miR-205 expressions and mRNA levels of ZEB1, E-cadherin, and α-SMA using real-time qPCR; the protein levels of ZEB1, ZEB2, E-cadherin, and α-SMA were detected with Western blotting. Immunohistochemistry was performed to examine the ectopic expression of β-catenin and E-cadherin expression in the cells.
  
  
  RESULTS
  The expression levels of ZEB1 and ZEB2 decreased significantly (P<0.01) while E-cadherin expression was up-regulated (P<0.01) in cells transfected with miR-205 mimics. Transfection with miR-205 mimics also markedly down-regulated the expression of α-SMA (P<0.01), a marker of mesenchymal cells that play an important role in EMT of HK-2 cells. The ectopic expression of β-catenin was inhibited by miR-205 mimics in HK-2 cells.
  
  
  CONCLUSIONS
  miR-205 inhibits EMT in HK-2 cells by down-regulating the expression levels of ZEB1 and ZEB2.
authors.affiliation: Central Laboratory, Ningde Municipal Hospital, Affiliated Hospital of Fujian Medical University, Ningde 352100, China. E-mail: 278512344@qq.com.
authors.forename: Luo-Yuan
authors.initials: LY
authors.lastname: Cao
authors.forename: Jing
authors.initials: J
authors.lastname: Yang
authors.forename: Xian-Guo
authors.initials: XG
authors.lastname: Fu
authors.forename: Ying-Hua
authors.initials: YH
authors.lastname: Lin
authors.forename: Feng
authors.initials: F
authors.lastname: Lin
authors.forename: Bao-Ying
authors.initials: BY
authors.lastname: Huang
mesh_terms: D000199:Actins; D015703:Antigens, CD; D015820:Cadherins; D015536:Down-Regulation; D058750:Epithelial-Mesenchymal Transition; D018398:Homeodomain Proteins; D006801:Humans; D007687:Kidney Tubules, Proximal / Q000378:metabolism*; D035683:MicroRNAs / Q000502:physiology*; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D015854:Up-Regulation; D000074165:Zinc Finger E-box Binding Homeobox 2 / Q000378:metabolism*; D000071799:Zinc Finger E-box-Binding Homeobox 1 / Q000378:metabolism*; D051176:beta Catenin
chemical_list: D000199:Actins; D015703:Antigens, CD; D015820:Cadherins; D018398:Homeodomain Proteins; D035683:MicroRNAs; D012333:RNA, Messenger; D012097:Repressor Proteins; D014157:Transcription Factors; D000074165:Zinc Finger E-box Binding Homeobox 2; D000071799:Zinc Finger E-box-Binding Homeobox 1; D051176:beta Catenin; C541116:ACTA2 protein, human; C513384:CDH1 protein, human; C495270:CTNNB1 protein, human; C534425:MIR205, human; C486307:ZEB1 protein, human; C492363:ZEB2 protein, human