title: Different effectiveness of two pastas supplemented with either lipophilic or hydrophilic/phenolic antioxidants in affecting serum as evaluated by the novel Antioxidant/Oxidant Balance approach.
abstract: Effectiveness in improving serum antioxidant status of two functional pastas was evaluated by the novel Antioxidant/Oxidant Balance (AOB) parameter, calculated as Antioxidant Capacity (AC)/Peroxide Level ratio, assessed here for the first time. In particular, Bran Oleoresin (BO) and Bran Water (BW) pastas, enriched respectively with either lipophilic (tocochromanols, carotenoids) or hydrophilic/phenolic antioxidants extracted from durum wheat bran, were studied. Notably, BO pasta was able to improve significantly (+65%) serum AOB during four hours after intake similarly to Lisosan G, a wheat antioxidant-rich dietary supplement. Contrarily, BW pasta had oxidative effect on serum so as conventional pasta and glucose, thus suggesting greater effectiveness of lipophilic than hydrophilic/phenolic antioxidants under our experimental conditions. Interestingly, no clear differences between the two pastas were observed, when AC measurements of either serum after pasta intake or pasta extracts by in vitro assays were considered, thus strengthening effectiveness and reliability of AOB approach.
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: maura.laus@unifg.it.
authors.forename: Maura N
authors.initials: MN
authors.lastname: Laus
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: mario.soccio@unifg.it.
authors.forename: Mario
authors.initials: M
authors.lastname: Soccio
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: michela.alfarano@unifg.it.
authors.forename: Michela
authors.initials: M
authors.lastname: Alfarano
authors.affiliation: Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari "Aldo Moro", Via Amendola, 165A, 70126 Bari, Italy. Electronic address: antonella.pasqualone@uniba.it.
authors.forename: Antonella
authors.initials: A
authors.lastname: Pasqualone
authors.affiliation: Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce-Monteroni, 73100 Lecce, Italy. Electronic address: marcello.lenucci@unisalento.it.
authors.forename: Marcello S
authors.initials: MS
authors.lastname: Lenucci
authors.affiliation: Dipartimento di Scienze Agrarie e Forestali, Università degli Studi di Palermo, Viale delle Scienze, 90128 Palermo, Italy; Fondazione Angelo e Salvatore Lima Mancuso, Piazza Marina, 61, 90100 Palermo, Italy. Electronic address: giuseppe.dimiceli@unipa.it.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Di Miceli
authors.affiliation: Dipartimento di Scienze Agrarie, degli Alimenti e dell'Ambiente, Università degli Studi di Foggia, Via Napoli, 25, 71122 Foggia, Italy. Electronic address: donato.pastore@unifg.it.
authors.forename: Donato
authors.initials: D
authors.lastname: Pastore
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000032:analysis* / Q000737:chemistry; D002338:Carotenoids; D019587:Dietary Supplements; D005260:Female; D006801:Humans; D008297:Male; D016877:Oxidants / Q000737:chemistry*; D010084:Oxidation-Reduction; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D015203:Reproducibility of Results; D044967:Serum / Q000737:chemistry*; D014908:Triticum / Q000737:chemistry*; D055815:Young Adult
chemical_list: D000975:Antioxidants; D016877:Oxidants; D010636:Phenols; D010936:Plant Extracts; C018620:oleoresins; D002338:Carotenoids
keywords: AAPH, 2,2′-azobis(2-methylpropionamidine) dihydrochloride (PubChem CID: 76344); ABTS, diammonium-2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (PubChem CID: 9570474); DPPD, N,N-diethyl-p-phenylenediamine (PubChem CID: 7120); Durum wheat antioxidants; Fluorescein, 3′,6′-dihydroxy-3H-spiro[2-benzofuran-1,9′-xanthen]-3-one (PubChem CID: 16850); Functional pasta; Human serum; LOX-FL; Linoleic acid sodium salt (PubChem CID: 23702140); Lisosan G; ORAC; TEAC; Trolox, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (PubChem CID: 40634).