title: QuEChERS Purification Combined with Ultrahigh-Performance Liquid Chromatography Tandem Mass Spectrometry for Simultaneous Quantification of 25 Mycotoxins in Cereals.
abstract: A method based on the QuEChERS (quick, easy, cheap, effective, rugged, and safe) purification combined with ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS), was optimized for the simultaneous quantification of 25 mycotoxins in cereals. Samples were extracted with a solution containing 80% acetonitrile and 0.1% formic acid, and purified with QuEChERS before being separated by a C18 column. The mass spectrometry was conducted by using positive electrospray ionization (ESI+) and multiple reaction monitoring (MRM) models. The method gave good linear relations with regression coefficients ranging from 0.9950 to 0.9999. The detection limits ranged from 0.03 to 15.0 µg·kg-1, and the average recovery at three different concentrations ranged from 60.2% to 115.8%, with relative standard deviations (RSD%) varying from 0.7% to 19.6% for the 25 mycotoxins. The method is simple, rapid, accurate, and an improvement compared with the existing methods published so far.
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. gwzx848@163.com.|Laboratory of Quality and Safety Risk Assessment for Cereal Products (Beijing), Ministry of Agriculture, Beijing 100081, China. gwzx848@163.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Sun
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. liweixi@caas.cn.|Laboratory of Quality and Safety Risk Assessment for Cereal Products (Beijing), Ministry of Agriculture, Beijing 100081, China. liweixi@caas.cn.
authors.forename: Weixi
authors.initials: W
authors.lastname: Li
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. zhangyan06@caas.cn.|Laboratory of Quality and Safety Risk Assessment for Cereal Products (Beijing), Ministry of Agriculture, Beijing 100081, China. zhangyan06@caas.cn.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. huxuexu@caas.cn.|Laboratory of Quality and Safety Risk Assessment for Cereal Products (Beijing), Ministry of Agriculture, Beijing 100081, China. huxuexu@caas.cn.
authors.forename: Xuexu
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. wuli5151@126.com.|Laboratory of Quality and Safety Risk Assessment for Cereal Products (Beijing), Ministry of Agriculture, Beijing 100081, China. wuli5151@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.affiliation: Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China. wangbujun@caas.cn.|Laboratory of Quality and Safety Risk Assessment for Cereal Products (Beijing), Ministry of Agriculture, Beijing 100081, China. wangbujun@caas.cn.
authors.forename: Bujun
authors.initials: B
authors.lastname: Wang
mesh_terms: D000085:Acetates / Q000737:chemistry; D002851:Chromatography, High Pressure Liquid; D005561:Formates / Q000737:chemistry; D057230:Limit of Detection; D000432:Methanol / Q000737:chemistry; D009183:Mycotoxins / Q000032:analysis* / Q000302:isolation & purification; D012275:Oryza / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014908:Triticum / Q000737:chemistry*; D003313:Zea mays / Q000737:chemistry*
chemical_list: D000085:Acetates; D005561:Formates; D009183:Mycotoxins; C030544:formic acid; C018824:ammonium acetate; D000432:Methanol
keywords: QuEChERS; cereals; mycotoxin; ultrahigh performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS)