title: Improvement of Huangqi Decoction on Endothelial Dysfunction in 5/6 Nephrectomized Rats.
abstract: OBJECTIVE
  Endothelial dysfunction is a major factor in the progression of chronic kidney disease, which correlates with oxidative stress and NO deficiency. Huangqi decoction (HQD) is a potential anti-oxidant ingredient in renoprotection. However, the underlying mechanisms remained identified. Therefore, we investigated whether HQD exhibit improvement in endothelial dysfunction in the 5/6 nephrectomy (Nx) rat model.
  
  
  METHODS
  Male Wistar rats (180 - 250 g) were divided into sham, Nx and Nx + HQD (0.05, 0.15 and 0.45 g/kg) group, respectively. Renal function and histology were examined with ELISA and Immunohistochemical analysis. Endothelium-dependent relaxation of rat aortas was investigated by isometric tension recordings. Oxidative stress and NO bioavailability were detected by ELISA, DHE-staining, DAF-2 staining and western blotting.
  
  
  RESULTS
  Compared with Nx rats, HQD treatment reversed the functional and structural changes of kidney significantly. Besides, endothelium-dependent relaxation of rat aortas was also improved by HQD treatment. NADPH oxidase and ROS generation were inhibited while NO bioavailability was enhanced.
  
  
  CONCLUSIONS
  HQD can act as a potent prescription for the treatment of endothelium related vascular complications.
authors.affiliation: Laboratory of Renal Disease, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chu
authors.forename: Li
authors.initials: L
authors.lastname: Wang
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Mao
authors.forename: Wen
authors.initials: W
authors.lastname: Peng
mesh_terms: D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000503:physiopathology; D001835:Body Weight / Q000187:drug effects; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000503:physiopathology*; D007668:Kidney / Q000473:pathology / Q000503:physiopathology / Q000601:surgery; D008297:Male; D019255:NADPH Oxidases / Q000378:metabolism; D009392:Nephrectomy; D009569:Nitric Oxide / Q000378:metabolism; D017208:Rats, Wistar; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D004365:Drugs, Chinese Herbal; D017382:Reactive Oxygen Species; C000614027:huangqi decoction; D009569:Nitric Oxide; D019255:NADPH Oxidases