title: [Application of whole genome sequencing technology in the epidemiology of tuberculosis].
abstract: Objective:To delineate the application of whole genome sequencing technology in the epidemiology of tuberculosis.Methods:From 2009 to 2012, nineMycobacterium tuberculosisthat sharing identical variable number of tandem repeats genotype (VNTR) patterns were reported from two TB cases designated hospitals. Both whole-genome sequencing analysis (WGS) and epidemiologic investigations were performed to describe the transmission patterns of theseMycobacterium tuberculosis.Results:By WGS analysis, two genomic clusters including 7 and 2Mycobacterium tuberculosiswere noticed, respectively. The cluster of 2 cases possessed more than 15 single nucleotide polymorphisms (SNPs) when compared to the cluster of 7 cases and suggesting that the transmission route was independent. The transmission chain based on the SNPs difference showed the process of the propagation direction and the accumulation of drug resistance mutations in each cluster.Conclusion:Using a WGS-based genomic epidemiologic approach, we were able to reconstruct the tuberculosis transmission network, tracing the putative source of the transmission and determining the transmission direction or the missing links.
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention, Shanghai 100336, China.
authors.forename: J
authors.initials: J
authors.lastname: Wu
authors.affiliation: Shanghai Minhang Center for Disease Control and Prevention, Shanghai 201101, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Tang
authors.affiliation: Fudan University, Shanghai 200032, China.
authors.forename: C G
authors.initials: CG
authors.lastname: Yang
authors.affiliation: Shanghai Minhang Center for Disease Control and Prevention, Shanghai 201101, China.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Yan
authors.affiliation: Shanghai Minhang Center for Disease Control and Prevention, Shanghai 201101, China.
authors.forename: H
authors.initials: H
authors.lastname: Sun
authors.affiliation: Shanghai Municipal Center for Disease Control and Prevention, Shanghai 100336, China.
authors.forename: X
authors.initials: X
authors.lastname: Shen
mesh_terms: D016680:Genome, Bacterial; D023281:Genomics; D005838:Genotype; D006801:Humans; D018598:Minisatellite Repeats; D017720:Molecular Epidemiology; D009169:Mycobacterium tuberculosis; D020641:Polymorphism, Single Nucleotide; D014376:Tuberculosis