title: Determination of oxolinic acid, danofloxacin, ciprofloxacin, and enrofloxacin in porcine and bovine meat by micellar liquid chromatography with fluorescence detection.
abstract: A method was developed for the determination of oxolinic acid, danofloxacin, ciprofloxacin and enrofloxacin by micellar liquid chromatography - fluorescence detection in commercial porcine and bovine meat. The samples were ultrasonicated in a micellar solution, free of organic solvent, to extract the analytes, and the supernatant was directly injected. The quinolones were resolved in <22min using a mobile phase of 0.05M SDS - 7.5% 1-propanol - 0.5% triethylamine buffered at pH 3, running through a C18 column at 1mL/min using isocratic mode. The method was validated by the in terms of: selectivity, calibration range (0.01-0.05 to 0.5mg/kg), linearity (r2>0.9998), trueness (89.3-105.1%), precision (<8.3%), decision limit (<12% over the maximum residue limit), detection capability (<21% over the maximum residue limit), ruggedness (<5.6%) and stability. The procedure was rapid, eco-friendly, safe and easy-to-handle.
authors.affiliation: Química Bioanalítica, QFA, ESTCE, Universitat Jaume I, 12071 Castelló, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Terrado-Campos
authors.affiliation: Química Bioanalítica, QFA, ESTCE, Universitat Jaume I, 12071 Castelló, Spain.
authors.forename: Khaled
authors.initials: K
authors.lastname: Tayeb-Cherif
authors.affiliation: Química Bioanalítica, QFA, ESTCE, Universitat Jaume I, 12071 Castelló, Spain. Electronic address: vicentej@uji.es.
authors.forename: Juan
authors.initials: J
authors.lastname: Peris-Vicente
authors.affiliation: Química Bioanalítica, QFA, ESTCE, Universitat Jaume I, 12071 Castelló, Spain.
authors.forename: Samuel
authors.initials: S
authors.lastname: Carda-Broch
authors.affiliation: Química Bioanalítica, QFA, ESTCE, Universitat Jaume I, 12071 Castelló, Spain.
authors.forename: Josep
authors.initials: J
authors.lastname: Esteve-Romero
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000032:analysis*; D002417:Cattle; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods*; D002939:Ciprofloxacin / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis*; D000077422:Enrofloxacin; D005453:Fluorescence; D024841:Fluoroquinolones / Q000032:analysis*; D005506:Food Contamination / Q000032:analysis; D008460:Meat / Q000032:analysis*; D008823:Micelles; D010093:Oxolinic Acid / Q000032:analysis*; D013552:Swine
chemical_list: D000890:Anti-Infective Agents; D024841:Fluoroquinolones; D008823:Micelles; C068581:danofloxacin; D000077422:Enrofloxacin; D002939:Ciprofloxacin; D010093:Oxolinic Acid
keywords: Flesh; Food safety; Micellar; Quinolones; Validation