title: Complete plastid genome ofEriobotrya japonica(Thunb.) Lindl and comparative analysis in Rosaceae.
abstract: Eriobotrya japonica(Thunb.) Lindl (loquat) is an evergreen Rosaceae fruit tree widely distributed in subtropical regions. Its leaves are considered as traditional Chinese medicine and are of high medical value especially for cough and emesis. Thus, we sequenced the complete plastid genome ofE. japonicato better utilize this important species. The complete plastid genome ofE. japonicais 159,137 bp in length, which contains a typical quadripartite structure with a pair of inverted repeats (IR, 26,326 bp) separated by large (LSC, 89,202 bp) and small (SSC, 19,283 bp) single-copy regions. TheE. japonicaplastid genome encodes 112 unique genes which consist of 78 protein-coding genes, 30 tRNA genes and 4 rRNA genes. Gene structure and content ofE. japonicaplastid genome are quite conserved and show similarity among Rosaceous species. Five large indels are unique toE. japonicain comparison withPyrus pyrifoliaandPrunus persica, which could be utilized as molecular markers. A total of 72 simple sequence repeats (SSRs) were detected and most of them are mononucleotide repeats composed of A or T, indicating a strong A or T bias for base composition. The Ka and Ks ratios of most genes are lower than 1, which suggests that most genes are under purifying selection. The phylogenetic analysis described the evolutionary relationship within Rosaceae and fully supported a close relationship betweenE. japonicaandP. pyrifolia.
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 China.
authors.forename: Liqun
authors.initials: L
authors.lastname: Shen
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 China.
authors.forename: Qijie
authors.initials: Q
authors.lastname: Guan
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 China.
authors.forename: Awais
authors.initials: A
authors.lastname: Amin
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 China.
authors.forename: Mengzhu
authors.initials: M
authors.lastname: Li
authors.affiliation: Changshu Qiushi Technology Co. Ltd., Changshu, 215500 China.
authors.forename: Ximin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027 China.
authors.forename: Jingkui
authors.initials: J
authors.lastname: Tian
keywords: Chloroplast genome; Eriobotrya; Gene evolution; Loquat; Rosaceae