title: Non-Thermal Atmospheric Pressure Plasma Efficiently Promotes the Proliferation of Adipose Tissue-Derived Stem Cells by Activating NO-Response Pathways.
abstract: Non-thermal atmospheric pressure plasma (NTAPP) is defined as a partially ionized gas with electrically charged particles at atmospheric pressure. Our study showed that exposure to NTAPP generated in a helium-based dielectric barrier discharge (DBD) device increased the proliferation of adipose tissue-derived stem cells (ASCs) by 1.57-fold on an average, compared with untreated cells at 72 h after initial NTAPP exposure. NTAPP-exposed ASCs maintained their stemness, capability to differentiate into adipocytes but did not show cellular senescence. Therefore, we suggested that NTAPP can be used to increase the proliferation of ASCs without affecting their stem cell properties. When ASCs were exposed to NTAPP in the presence of a nitric oxide (NO) scavenger, the proliferation-enhancing effect of NTAPP was not obvious. Meanwhile, the proliferation of NTAPP-exposed ASCs was not much changed in the presence of scavengers for reactive oxygen species (ROS). Also, Akt, ERK1/2, and NF-κB were activated in ASCs after NTAPP exposure. These results demonstrated that NO rather than ROS is responsible for the enhanced proliferation of ASCs following NTAPP exposure. Taken together, this study suggests that NTAPP would be an efficient tool for use in the medical application of ASCs both in vitro and in vivo.
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.
authors.forename: Jeongyeon
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Electrical Engineering, Pusan National University, Pusan 46241, Korea.
authors.forename: Hyunyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Electrical Engineering, Pusan National University, Pusan 46241, Korea.
authors.forename: Hae June
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Oral Anatomy, School of Dentistry, Pusan National University, Yangsan 50612, Korea.
authors.forename: Gyoo Cheon
authors.initials: GC
authors.lastname: Kim
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Yonsei University College of Medicine, Seoul 03722, Korea.
authors.forename: Do Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Batang Plastic Surgery Center, Gangnam-Gu, Seoul 06120, Korea.
authors.forename: Sungbum
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Biochemistry, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Korea.
authors.forename: Kiwon
authors.initials: K
authors.lastname: Song
mesh_terms: D000273:Adipose Tissue / Q000166:cytology; D049109:Cell Proliferation / Q000187:drug effects*; D006801:Humans; D009569:Nitric Oxide / Q000378:metabolism*; D058626:Plasma Gases / Q000378:metabolism*; D015398:Signal Transduction; D013234:Stem Cells / Q000187:drug effects* / Q000502:physiology*
chemical_list: D058626:Plasma Gases; D009569:Nitric Oxide