title: Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies.
abstract: Golden pompano Trachinotus ovatus, a popularly cultured and commercially important marine fish worldwide, has been recognized as a promising candidate for mariculture. However, outbreaks of infectious bacterial or viral diseases and environmental deterioration have led to great economic losses in T. ovatus aquaculture recently. In our research, we established a new mid-kidney cell line, designated as TOK, from golden pompano, T. ovatus. The optimized growth temperature and working concentration of fetal bovine serum (FBS) were 28°C and 10-20%, respectively. Foreign genes could express well in TOK cells. The modal number of TOK cells was 54. The TOK cells were susceptive to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV), and the virus could propagate in cells. Propagation was verified by qRT-PCR, and virions were observed under electron microscopy. Cytotoxicity analysis revealed that TOK cells were sensitive to different concentrations of extracellular products (ECPs) from Vibrio alginolyticus and V. anguillarum. Moreover, heavy metals (Cd, Cu, and Hg) also showed dose-dependent cytotoxicity to the TOK cell line. We established a mid-kidney cell line from T. ovatus which could be applied to cytotoxicity assays of heavy metals. The newly established TOK cell line possesses great application potential in genetic manipulation, virus-host interaction studies, and toxicity assays of bacterial extracellular products and heavy metals.
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Lingli
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynethesis Chemistry, Guangxi Academy of Sciences, Nanning, 530007, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Songwei
authors.initials: S
authors.lastname: Ni
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.|University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing, 100049, China.
authors.forename: Yepin
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China. weisn@scsio.ac.cn.
authors.forename: Shina
authors.initials: S
authors.lastname: Wei
authors.affiliation: Key Laboratory of Tropical Marine Bio-resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou, 510301, China. qinqiwei64@outlook.com.|College of Marine Sciences, South China Agricultural University, 483 Wushan Road, Guangzhou, 510642, China. qinqiwei64@outlook.com.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Qin
mesh_terms: D000818:Animals; D001483:Base Sequence; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line / Q000166:cytology*; D049109:Cell Proliferation / Q000187:drug effects; D004198:Disease Susceptibility; D005393:Fish Diseases / Q000821:virology; D007668:Kidney / Q000166:cytology* / Q000648:ultrastructure; D019216:Metals, Heavy / Q000633:toxicity; D008954:Models, Biological; D030381:Nodaviridae / Q000502:physiology*; D010473:Perciformes / Q000821:virology*; D018675:Toxicity Tests; D014162:Transfection; D014779:Virus Replication / Q000187:drug effects
chemical_list: D019216:Metals, Heavy
keywords: Cytotoxicity; Heavy metals; Mid-kidney cell line; Nervous necrosis virus; Trachinotus ovatus