title: Genotypic analysis of Escherichia coli strains that cause urosepsis in the Aegean region.
abstract: OBJECTIVE
  The aim of this study was to characterize strains genotypically, to determine their phylogenetic relationships, to investigate the presence of the papG gene, and to compare their antibiotic susceptibility test results.
  
  
  METHODS
  Seventy pathogenic E. coli strains were isolated from both urine and blood cultures of patients with the preliminary diagnosis of urosepsis who were referred to the Ege University Faculty of Medicine, Bacteriology Laboratory of Medical Microbiology Department in İzmir. All of these strains were examined for the papG gene and phylogenetic groups with the multiplex polymerase chain reaction technique. Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) were used for epidemiologic analysis.
  
  
  RESULTS
  Phylogenetically, it was found that 16 belonged to group B2, 31 belonged to group D, 15 belonged to group A, and 7 belonged to group B1. One strain was not identified as belonging to a group. papG genes were found in 26 of 70 E. coli strains. Thirty urosepsis pathogenic E. coli strains were analyzed with MLST. Twenty-two strains were identified as new STs.
  
  
  CONCLUSIONS
  These findings are extremely important for Turkey and these new 22 strains should be investigated in more detail because they are new and have the potential to lead to infections.
authors.affiliation: Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir, Turkey.
authors.forename: Betül
authors.initials: B
authors.lastname: Giray
authors.affiliation: Department of Biology, Basic and Industrial Microbiology Section, Faculty of Science, Ege University, İzmir, Turkey.
authors.forename: Füsun Bahriye
authors.initials: FB
authors.lastname: Uçar
authors.affiliation: Department of Medical Microbiology, Faculty of Medicine, Ege University, İzmir, Turkey.
authors.forename: Sabire Şöhret
authors.initials: SŞ
authors.lastname: Aydemir
mesh_terms: D016521:Electrophoresis, Gel, Pulsed-Field; D004926:Escherichia coli; D004927:Escherichia coli Infections; D005838:Genotype; D006801:Humans; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D014421:Turkey
keywords: E. coli; PFGE; papG; phylogenetic grouping; urosepsis