title: Neuropeptide Y Y5receptor localization in mouse central nervous system.
abstract: Neuropeptide Y (NPY) and its receptors affect blood pressure, feeding behavior, and neurogenesis. In this study, the distribution of neurons expressing NPY Y5receptor (Y5) was examined in adult mouse central nervous system by immunohistochemistry. Y5protein localization was investigated using polyclonal anti-Y5antibody, which was successfully preabsorbed with Y5knockout brain tissues. The preabsorbed anti-Y5antibody did not react with Y5knockout brain tissues, thus meeting the "hard specificity criterion," which is the absence of staining in tissues genetically deficient for the antigen (Pradidarcheep et al., 2008). Y5-positive neurons were found in most brain areas. Most Y5immunoreactivities were observed as dot-like structures adjacent to the plasma membrane, as expected for a cell membrane receptor. In situ hybridization showed that the Y5mRNA expression was correlated with the Y5protein level in each case and that it was probably controlled by the transcriptional regulation of the Y5gene. In the nuclei where Y5was expressed, Y5immunoreactivities were found mainly in the somatic and dendritic areas. The distribution patterns of the Y5-positive cells that were broader than previously expected suggest important biological activities of the Y5in many brain areas.
authors.affiliation: Division of Pharmacology, Niigata University, Graduate School of Medical and Dental Sciences, Niigata 951-8510, Japan. Electronic address: murase@med.niigata-u.ac.jp.
authors.forename: Shin-Ichi
authors.initials: SI
authors.lastname: Murase
authors.affiliation: Division of Pharmacology, Niigata University, Graduate School of Medical and Dental Sciences, Niigata 951-8510, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Shiiya
authors.affiliation: Division of Pharmacology, Niigata University, Graduate School of Medical and Dental Sciences, Niigata 951-8510, Japan. Electronic address: hhiguchi@med.niigata-u.ac.jp.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Higuchi
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D005455:Fluorescent Antibody Technique; D005968:Glutamate Decarboxylase / Q000378:metabolism; D017403:In Situ Hybridization; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D019006:Neural Cell Adhesion Molecules / Q000378:metabolism; D009474:Neurons / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000235:genetics / Q000378:metabolism*
chemical_list: D019006:Neural Cell Adhesion Molecules; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; D005968:Glutamate Decarboxylase; C402565:glutamate decarboxylase 1
keywords: G-protein coupled receptor; Knockout mouse, obesity; Specificity of antibody