title: Suppression of miR-628-3p and miR-641 is involved in rifampin-mediated CYP3A4 induction in HepaRG cells.
abstract: OBJECTIVE
  This study aimed to explore the role of miRNAs in rifampin-mediated induction of CYP3A4 in HepaRG cells.
  
  
  METHODS
  Microarray was performed to determine the expression of miRNAs in rifampin-treated HepaRG cells, followed by bioinformatics and luciferase reporter gene assay to analyze miRNAs that directly target CYP3A4. Overexpression of miRNA mimics was used to study their effects on CYP3A4 induction.
  
  
  RESULTS
  Forty-seven miRNAs were suppressed and 18 miRNAs were increased by rifampin (above twofold). MiR-628-3p and miR-641 repressed the 3'-UTR luciferase activity of CYP3A4. Overexpression of miR-628-3p and miR-641 showed significant decrease of CYP3A4 mRNA level as well as CYP3A4 induction by rifampin.
  
  
  CONCLUSIONS
  miR-628-3p and miR-641 could directly target CYP3A4 and are negatively regulated in CYP3A4 induction by rifampin.
authors.affiliation: Department of Pharmacology, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Pharmacology, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Jingyang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Forensic Medicine, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Pharmacology, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Nie
authors.affiliation: Department of Pharmaceutical Sciences, Center for Pharmacogenetics, School of Pharmacy, University of Pittsburgh, Pennsylvania 15261, USA.
authors.forename: Xiaochao
authors.initials: X
authors.lastname: Ma
authors.affiliation: The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
authors.forename: Quancheng
authors.initials: Q
authors.lastname: Kan
authors.affiliation: Department of Pharmacology, Basic Medical College, Zhengzhou University, Zhengzhou, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhang
mesh_terms: D051544:Cytochrome P-450 CYP3A / Q000096:biosynthesis*; D065701:Cytochrome P-450 CYP3A Inducers / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D035683:MicroRNAs / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D012293:Rifampin / Q000494:pharmacology*
chemical_list: D065701:Cytochrome P-450 CYP3A Inducers; C000589022:MIRN628 microRNA, human; C000591152:MIRN641 microRNA, human; D035683:MicroRNAs; D051544:Cytochrome P-450 CYP3A; C510163:CYP3A4 protein, human; D012293:Rifampin
keywords: CYP3A4; induction; miRNA; post-transcriptional regulation; rifampin