title: MALDI-TOF mass spectrometry for rapid diagnosis of postoperative endophthalmitis.
abstract: This study describes an innovative strategy for rapid detection and identification of bacteria causing endophthalmitis, combining the use of an automated blood culture system with MALDI-TOF mass spectrometry methodology. Using this protocol, we could identify 96% of 45 bacterial strains isolated from vitreous samples collected in acute post-operative endophthalmitis patients.
authors.affiliation: Department of Ophthalmology, Grenoble Alpes University Hospital, France.
authors.forename: Adriane
authors.initials: A
authors.lastname: Mailhac
authors.affiliation: Braintech Lab, UMR1205, Grenoble Alpes University, France.
authors.forename: Harmonie
authors.initials: H
authors.lastname: Durand
authors.affiliation: Bacteriological Laboratory, Department of Infectious Agents, Biology Institute, Grenoble Alpes University Hospital, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Boisset
authors.affiliation: Parasitology and Mycology Laboratory, Department of Infectious Agents, Biology Institute, Grenoble Alpes University Hospital, France; TIMC-IMAG-TheREx, UMR 5525 CNRS-UGA, Grenoble Alpes University, France.
authors.forename: Danièle
authors.initials: D
authors.lastname: Maubon
authors.affiliation: Braintech Lab, UMR1205, Grenoble Alpes University, France.
authors.forename: Francois
authors.initials: F
authors.lastname: Berger
authors.affiliation: Bacteriological Laboratory, Department of Infectious Agents, Biology Institute, Grenoble Alpes University Hospital, France.
authors.forename: Max
authors.initials: M
authors.lastname: Maurin
authors.affiliation: Department of Ophthalmology, Grenoble Alpes University Hospital, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Chiquet
authors.affiliation: Braintech Lab, UMR1205, Grenoble Alpes University, France; Clinical Proteomic and Transcriptomic Plateform, Research Department, Grenoble Alpes University Hospital, France; Biochemistry and Genetic Laboratory, Department of Biochemistry, Toxicology and Pharmacology, Biology Institute, Grenoble Alpes University, France. Electronic address: MBidart@chu-grenoble.fr.
authors.forename: Marie
authors.initials: M
authors.lastname: Bidart
mesh_terms: D001419:Bacteria / Q000302:isolation & purification*; D001431:Bacteriological Techniques / Q000379:methods; D009877:Endophthalmitis / Q000175:diagnosis* / Q000382:microbiology*; D057166:High-Throughput Screening Assays; D006801:Humans; D011183:Postoperative Complications / Q000175:diagnosis / Q000382:microbiology*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods; D014822:Vitreous Body / Q000382:microbiology
keywords: Blood culture; Direct identification; Endophthalmitis; MALDI-TOF mass spectrometry; Staphylococcus; Vitreous