title: Oxyfunctionalization of pyridine derivatives using whole cells of Burkholderia sp. MAK1.
abstract: Pyridinols and pyridinamines are important intermediates with many applications in chemical industry. The pyridine derivatives are in great demand as synthons for pharmaceutical products. Moreover, pyridines are used either as biologically active substances or as building blocks for polymers with unique physical properties. Application of enzymes or whole cells is an attractive strategy for preparation of hydroxylated pyridines since the methods for chemical synthesis of pyridinols, particularly aminopyridinols, are usually limited or inefficient. Burkholderia sp. MAK1 (DSM102049), capable of using pyridin-2-ol as the sole carbon and energy source, was isolated from soil. Whole cells of Burkholderia sp. MAK1 were confirmed to possess a good ability to convert different pyridin-2-amines and pyridin-2-ones into their 5-hydroxy derivatives. Moreover, several methylpyridines as well as methylated pyrazines were converted to appropriate N-oxides. In conclusion, regioselective oxyfunctionalization of pyridine derivatives using whole cells of Burkholderia sp. MAK1 is a promising method for the preparation of various pyridin-5-ols and pyridin-N-oxides.
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, the Life Sciences Centre, Vilnius University, Sauletekio al. 7, LT-10257 Vilnius, Lithuania.
authors.forename: Jonita
authors.initials: J
authors.lastname: Stankevičiūtė
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, the Life Sciences Centre, Vilnius University, Sauletekio al. 7, LT-10257 Vilnius, Lithuania.
authors.forename: Justas
authors.initials: J
authors.lastname: Vaitekūnas
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, the Life Sciences Centre, Vilnius University, Sauletekio al. 7, LT-10257 Vilnius, Lithuania.
authors.forename: Vytautas
authors.initials: V
authors.lastname: Petkevičius
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, the Life Sciences Centre, Vilnius University, Sauletekio al. 7, LT-10257 Vilnius, Lithuania.
authors.forename: Renata
authors.initials: R
authors.lastname: Gasparavičiūtė
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, the Life Sciences Centre, Vilnius University, Sauletekio al. 7, LT-10257 Vilnius, Lithuania.
authors.forename: Daiva
authors.initials: D
authors.lastname: Tauraitė
authors.affiliation: Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, the Life Sciences Centre, Vilnius University, Sauletekio al. 7, LT-10257 Vilnius, Lithuania.
authors.forename: Rolandas
authors.initials: R
authors.lastname: Meškys
mesh_terms: D019117:Burkholderia / Q000254:growth & development* / Q000302:isolation & purification; D006900:Hydroxylation; D015394:Molecular Structure; D011725:Pyridines / Q000737:chemistry*; D012988:Soil Microbiology
chemical_list: D011725:Pyridines