title: DAZ-interacting Protein 1 (Dzip1) Phosphorylation by Polo-like Kinase 1 (Plk1) Regulates the Centriolar Satellite Localization of the BBSome Protein during the Cell Cycle.
abstract: The function of the primary cilia, which is assembled in most vertebrate cells, is achieved by transport in and out of kinds of signaling receptors. The BBSome protein complex could recognize and target membrane proteins to the cilia, but how the BBSome itself is transported into the cilia is poorly understood. Here we demonstrate that the centrosome protein Dzip1 mediates the assembly of the BBSome-Dzip1-PCM1 complex in the centriolar satellites (CS) at the G0phase for ciliary translocation of the BBSome. Phosphorylation of Dzip1 at Ser-210 by Plk1 (polo-like kinase 1) during the G2phase promotes disassembly of this complex, resulting in removal of Dzip1 and the BBSome from the CS. Inhibiting the kinase activity of Plk1 maintains the CS localization of the BBSome and Dzip1 at the G2phase. Collectively, our findings reveal the cell cycle-dependent regulation of BBSome transport to the CS and highlight a potential mechanism that the BBSome-mediated signaling pathways are accordingly regulated during the cell cycle.
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: Boyan
authors.initials: B
authors.lastname: Zhang
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Xu
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: Sisi
authors.initials: S
authors.lastname: Yang
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: Tenghan
authors.initials: T
authors.lastname: Zhuang
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: Guopeng
authors.initials: G
authors.lastname: Wang
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: He
authors.initials: H
authors.lastname: Ren
authors.affiliation: the Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing 210029, China.
authors.forename: Steven Y
authors.initials: SY
authors.lastname: Cheng
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and.
authors.forename: Qing
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: From the Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and the State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871 and zhangcm@pku.edu.cn.
authors.forename: Chuanmao
authors.initials: C
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002502:Centrioles / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D016195:G2 Phase / Q000502:physiology*; D057809:HEK293 Cells; D006801:Humans; D051379:Mice; D041681:NIH 3T3 Cells; D010766:Phosphorylation; D017346:Protein Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000502:physiology; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D000097869:Polo-Like Kinase 1
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D004268:DNA-Binding Proteins; D017346:Protein Serine-Threonine Kinases; D011518:Proto-Oncogene Proteins; D000097869:Polo-Like Kinase 1; C492524:DZIP1 protein, human; C511358:Dzip1 protein, mouse
keywords: cell cycle; centrosome; primary cilium; protein phosphorylation; protein translocation; serine/threonine protein kinase