title: Effects of oncostatin M on cell proliferation and osteogenic differentiation in C3H10T1/2.
abstract: To explore the effects of protein factor Oncostatin M (OSM), a member of the Interleukin-6 (IL-6) family on cell proliferation, osteogenic differentiation and mineralization.
  
  
  
  Basal nutrient solutions of different concentrations of OSM (0, 5, 10, 20, 40, 80 ng/ml) were used. In order to divide embryonic origin between mesenchymal stem cells C3H10T1/2 of in vitro cultured mice, and the effects of in vitro proliferation efficiencies of C3H10T1/2 cells of different concentrations of OSM, the C3H10T1/2 cells were divided into four groups: (1) Basal nutrient solution group (negative control); (2) Osteogenesis induced liquid group (positive control); (3) OSM (20 ng/ml) group; (4) Experimental group (osteogenesis induced liquid + OSM (20 ng/ml)). The expressions levels of relevant osteogenesis and mineralization genes were detected.
  
  
  
  OSM had several effects on promoting the proliferation of embryonic origin mesenchymal stem cells C3H10T1/2 with respect to time of exposure as well as concentrations. In the present study, it has been shown that when the concentration of OSM is 20 ng/ml, the effects of promoting proliferation are most obvious. OSM can induce osteogenic differentiation of C3H10T1/2, make the process of osteogenic differentiation in advance, and promote the formation of end-stage calcium deposits and mineralized nodule, and osteogenic differentiation of C3H10T1/2 is finally achieved.
  
  
  
  OSM can promote the proliferation of C3H10T1/2, and induce its osteogenic differentiation and end-stage mineralization.
authors.affiliation: Department of Orthopeadics, Xiangyang Hospital, Hubei University of Medicine, Xiangyang, Hubei Province, 441001, China.
authors.forename: F
authors.initials: F
authors.lastname: Zou
authors.forename: J-C
authors.initials: JC
authors.lastname: Xu
authors.forename: G-H
authors.initials: GH
authors.lastname: Wu
authors.forename: L-L
authors.initials: LL
authors.lastname: Zhou
authors.forename: Q-D
authors.initials: QD
authors.lastname: Wa
authors.forename: J-Q
authors.initials: JQ
authors.lastname: Peng
authors.forename: X-N
authors.initials: XN
authors.lastname: Zou
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects*; D051379:Mice; D053683:Oncostatin M / Q000494:pharmacology*; D010012:Osteogenesis / Q000187:drug effects*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: D053683:Oncostatin M