title: Electrosteric stabilization of colloidal TiO2nanoparticles with DNA and polyethylene glycol for selective enhancement of UV detection sensitivity in capillary electrophoresis analysis.
abstract: A new approach to selectively enhance the ultraviolet (UV) detection sensitivity of titania (TiO2), albeit in the presence of silica (SiO2), alumina (Al2O3), and zinc oxide (ZnO), nanoparticles in capillary electrophoresis (CE) analysis was developed. Interactions of Triton X-100 (TX-100), polyethylene glycol (PEG), and deoxyribonucleic acid (DNA) with TiO2nanoparticles produced larger CE-UV peaks at various enhancement factors. Single-stranded DNA (ssDNA) was a more effective adsorbate than double-stranded DNA (dsDNA) due to its flexible molecular structure that participated in a stronger interaction with TiO2nanoparticles via its sugar-phosphate backbone. Disaggregation of TiO2nanoparticles upon DNA binding due to electrosteric stabilization was validated using dynamic light scattering. PEG coating of TiO2-DNA nanoparticles further enhanced the UV detection sensitivity in CE analysis by providing extra electrosteric stabilization. This analytical technique, which involves binding of TiO2nanoparticles with DNA followed by coating with PEG, has allowed us to achieve progressively an enhancement factor up to 13.0 ± 3.0 - fold in analytical sensitivity for the accurate determination of disaggregated TiO2nanoparticles. Graphical Abstract Selective enhancement of UV detection sensitivity for TiO2nanoparticles via electrosteric stabilization using ssDNA and PEG.
authors.affiliation: Ottawa-Carleton Chemistry Institute, Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada.
authors.forename: Samar
authors.initials: S
authors.lastname: Alsudir
authors.affiliation: Ottawa-Carleton Chemistry Institute, Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario, K1S 5B6, Canada. edward.lai@carleton.ca.
authors.forename: Edward P C
authors.initials: EPC
authors.lastname: Lai
mesh_terms: D000327:Adsorption; D003102:Colloids; D004247:DNA / Q000737:chemistry*; D019075:Electrophoresis, Capillary / Q000379:methods*; D053768:Metal Nanoparticles; D011092:Polyethylene Glycols / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet / Q000379:methods*; D014025:Titanium / Q000737:chemistry*
chemical_list: D003102:Colloids; C009495:titanium dioxide; D011092:Polyethylene Glycols; D004247:DNA; D014025:Titanium
keywords: Deoxyribonucleic acid; Disaggregation; Electrosteric stabilization; Polyethylene glycol; Titanium dioxide; Triton X-100