title: Zinc Up-Regulates Insulin Secretion from β Cell-Like Cells Derived from Stem Cells from Human Exfoliated Deciduous Tooth (SHED).
abstract: Stem cells from human exfoliated deciduous tooth (SHED) offer several advantages over other stem cell sources. Using SHED, we examined the roles of zinc and the zinc uptake transporter ZIP8 (Zrt- and irt-like protein 8) while inducing SHED into insulin secreting β cell-like stem cells (i.e., SHED-β cells). We observed that ZIP8 expression increased as SHED differentiated into SHED-β cells, and that zinc supplementation at day 10 increased the levels of most pancreatic β cell markers-particularly Insulin and glucose transporter 2 (GLUT2). We confirmed that SHED-β cells produce insulin successfully. In addition, we note that zinc supplementation significantly increases insulin secretion with a significant elevation of ZIP8 transporters in SHED-β cells. We conclude that SHED can be converted into insulin-secreting β cell-like cells as zinc concentration in the cytosol is elevated. Insulin production by SHED-β cells can be regulated via modulation of zinc concentration in the media as ZIP8 expression in the SHED-β cells increases.
authors.affiliation: School of Medicine Department of Obstetrics, Gynecology and Reproductive Sciences, University of Maryland, Baltimore, MD 21201, USA. gykim@fpi.umaryland.edu.
authors.forename: Gyuyoup
authors.initials: G
authors.lastname: Kim
authors.affiliation: UCLA School of Dentistry, Los Angeles, CA 90095, USA. kshin@dentistry.ucla.edu.
authors.forename: Ki-Hyuk
authors.initials: KH
authors.lastname: Shin
authors.affiliation: Department of Orthodontics and Pediatric Dentistry, School of Dentistry, University of Maryland, Baltimore, MD 21201, USA. epae@umaryland.edu.
authors.forename: Eung-Kwon
authors.initials: EK
authors.lastname: Pae
mesh_terms: D027682:Cation Transport Proteins / Q000378:metabolism; D019070:Cell Lineage / Q000187:drug effects; D003600:Cytosol / Q000187:drug effects / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology; D019587:Dietary Supplements; D005819:Genetic Markers; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects / Q000378:metabolism*; D010513:Periodontal Ligament / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013234:Stem Cells / Q000166:cytology*; D014080:Tooth Exfoliation / Q000473:pathology*; D014094:Tooth, Deciduous / Q000166:cytology*; D014157:Transcription Factors / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects*; D015032:Zinc / Q000494:pharmacology*
chemical_list: D027682:Cation Transport Proteins; D005819:Genetic Markers; D007328:Insulin; D012333:RNA, Messenger; C530151:SLC39A8 protein, human; D014157:Transcription Factors; D015032:Zinc
keywords: Zrt- and irt-like protein (ZIP); dental pulp; insulin; stem cells; zinc