title: Enantiomeric separation of pharmaceutically important drug intermediates using a Metagenomic lipase and optimization of its large scale production.
abstract: In the present study, efficient enzymatic methods were developed using a recombinant metagenomic lipase (LipR1) for the synthesis of corresponding esters by the transesterification of five different pharmaceutically important secondary alcohols. The recombinant lipase (specific activity=87m6U/mg) showed maximum conversion in presence of ionic liquid with Naphthyl-ethanol (eeP=99%), Indanol and Methyl-4 pyridine methanol (eeS of 98% and 99%) respectively in 1h. Vinyl acetate was found as suitable acyl donor in transesterification reactions. It was interesting to observe that maximum eeP of 85% was observed in just 15min with 1-indanol. As this enzyme demonstrated pharmaceutical applications, attempts were made to scale up the enzyme production on a pilot scale in a 5litre bioreactor. Different physical parameters affecting enzyme production and biomass concentration such as agitation rate, aeration rate and inoculum concentration were evaluated. Maximum lipase activity of 8463U/ml was obtained at 7h of cultivation at 1 lpm, 300rpm and 1.5% inoculum.
authors.affiliation: Department of Biotechnology, Sector 25, Panjab University, Chandigarh 160014, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062 Punjab, India.
authors.forename: Linga
authors.initials: L
authors.lastname: Banoth
authors.affiliation: Department of Pharmaceutical Technology (Biotechnology), National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar 160062 Punjab, India.
authors.forename: Uttam Chand
authors.initials: UC
authors.lastname: Banerjee
authors.affiliation: Department of Biotechnology, Sector 25, Panjab University, Chandigarh 160014, India. Electronic address: jagsekhon@yahoo.com.
authors.forename: Jagdeep
authors.initials: J
authors.lastname: Kaur
mesh_terms: D055162:Biocatalysis; D019149:Bioreactors; D002175:Candida / Q000737:chemistry / Q000201:enzymology; D005591:Chemical Fractionation / Q000379:methods*; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000031:analogs & derivatives* / Q000737:chemistry / Q000302:isolation & purification; D005285:Fermentation; D005656:Fungal Proteins / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D015870:Gene Expression; D007189:Indans / Q000737:chemistry / Q000302:isolation & purification*; D052578:Ionic Liquids / Q000737:chemistry; D008049:Lipase / Q000096:biosynthesis / Q000737:chemistry* / Q000235:genetics; D054892:Metagenome; D009281:Naphthalenes / Q000737:chemistry / Q000302:isolation & purification*; D009540:Nicotinyl Alcohol / Q000737:chemistry / Q000302:isolation & purification*; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D013237:Stereoisomerism; D014753:Vinyl Compounds / Q000737:chemistry
chemical_list: C521161:1-(1'-naphthyl)ethanol; D005656:Fungal Proteins; D007189:Indans; D052578:Ionic Liquids; D009281:Naphthalenes; D011994:Recombinant Proteins; D014753:Vinyl Compounds; D000431:Ethanol; C016515:1-indanol; D009540:Nicotinyl Alcohol; D008049:Lipase; C011566:vinyl acetate
keywords: Fermentation; Indanol; Ionic liquid; Lipase; Naphthyl ethanol; aeration; agitation; kinetic resolution; transesterification