title: Alkaline Peroxides Versus Sodium Hypochlorite for Removing Denture Biofilm: a Crossover Randomized Trial.
abstract: This study evaluated the efficacy of cleanser solutions on denture biofilm removal by a crossover randomized clinical trial. Thirty two edentulous patients were instructed to brush their dentures (specific brush and liquid soap) three times a day (after breakfast, lunch and dinner) and to soak them (≥ 8 h) in: (C) control -water; (AP): alkaline peroxide; or (SH) 0.5% sodium hypochlorite. Each solution was used for 21 days (three cycles of 7 days). At the end of each cycle, the inner surfaces of maxillary dentures were disclosed (1% neutral red) and photographed (HX1 - Sony). Areas (total and stained biofilm) were measured (Image Tool software) and the percentage of biofilm calculated as the ratio between the area of the biofilm multiplied by 100 and total surface area of the internal base of the denture. Data were compared by means of generalized estimating equation (α=5%) and multiple comparisons (Bonferroni; α=1.67%). Immersion in SH reduced biofilm (%) (8.3 ± 13.3B) compared to C (18.2 ± 14.9A) and AP (18.2 ± 16.6A). The 0.5% sodium hypochlorite solution was the most efficacious for biofilm removal. Alkaline peroxides may not lead to further biofilm removal in patients with adequate denture maintenance habits.
authors.affiliation: Department of Dental Materials and Prosthetics, School of Dentistry of Ribeirão Preto, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Amanda
authors.initials: A
authors.lastname: Peracini
authors.affiliation: School of Dentistry, Federal University of Ceará, Fortaleza, CE, Brazil.
authors.forename: Rômulo Rocha
authors.initials: RR
authors.lastname: Regis
authors.affiliation: Department of Dental Materials and Prosthetics, School of Dentistry of Ribeirão Preto, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Raphael Freitas de
authors.initials: RF
authors.lastname: Souza
authors.affiliation: Department of Dental Materials and Prosthetics, School of Dentistry of Ribeirão Preto, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Valéria Oliveira
authors.initials: VO
authors.lastname: Pagnano
authors.affiliation: Department of Dental Materials and Prosthetics, School of Dentistry of Ribeirão Preto, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Cláudia Helena Lovato da
authors.initials: CH
authors.lastname: Silva
authors.affiliation: Department of Dental Materials and Prosthetics, School of Dentistry of Ribeirão Preto, USP - University of São Paulo, Ribeirão Preto, SP, Brazil.
authors.forename: Helena de Freitas Oliveira
authors.initials: HF
authors.lastname: Paranhos
mesh_terms: D018441:Biofilms; D018592:Cross-Over Studies; D003778:Dentures / Q000382:microbiology*; D005260:Female; D006801:Humans; D008297:Male; D010545:Peroxides / Q000008:administration & dosage*; D012973:Sodium Hypochlorite / Q000008:administration & dosage*
chemical_list: D010545:Peroxides; D012973:Sodium Hypochlorite