title: [Assessment of amylase and lipase levels following puncture biopsy and fine needle aspiration guided by endoscopic ultrasound in pancreatic lesions].
abstract: BACKGROUND
  Puncture biopsy and fine needle aspiration guided by endoscopic ultrasound has been used as an effective technique and is quickly becoming the procedure of choice for diagnosis and staging in patients suspected of having pancreatic cancer. This procedure has replaced retrograde cholangiopancreatography and brush cytology due to its higher sensitivity for diagnosis, and lower risk of complications.
  
  
  OBJECTIVE
  To assess the levels of pancreatic enzymes amylase and lipase, after the puncture biopsy and fine needle aspiration guided by endoscopic ultrasound in pancreatic lesions and the frequency of post-puncture acute pancreatitis.
  
  
  METHODS
  A longitudinal and descriptive study of consecutive cases was performed on outpatients submitted to puncture biopsy and fine needle aspiration guided by endoscopic ultrasound in pancreatic lesions. Levels of pancreatic enzymes such as amylase and lipase were measured before and after the pancreatic puncture. Finally we documented post-puncture pancreatitis cases.
  
  
  RESULTS
  A total of 100 patients who had been diagnosed with solid and cystic lesions were included in the study. Significant elevation was found at twice the reference value for lipase in 5 cases (5%) and for amylase in 2 cases (2%), none had clinical symptoms of acute pancreatitis. Eight (8%) of patients presented with mild nonspecific pain with no enzyme elevation compatible with pancreatitis.
  
  
  CONCLUSIONS
  Pancreatic biopsy needle aspiration guided by endoscopic ultrasound was associated with a low rate of elevated pancreatic enzymes and there were no cases of post-puncture pancreatitis.
authors.affiliation: Unidad de Endoscopía y Ultrasonido Endoscópico, Hospital de Especialidades del Centro Médico Nacional Siglo XXI, Ciudad de México, México. Electronic address: membriale@yahoo.com.mx.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Membrillo-Romero
authors.affiliation: Unidad de Endoscopía y Ultrasonido Endoscópico, Hospital de Especialidades del Centro Médico Nacional Siglo XXI, Ciudad de México, México.
authors.forename: Rubén
authors.initials: R
authors.lastname: Gonzalez-Lanzagorta
authors.affiliation: Unidad de Anestesiología, Hospital de Especialidades del Centro Médico Nacional Siglo XXI, Ciudad de México, México.
authors.forename: Dulce María
authors.initials: DM
authors.lastname: Rascón-Martínez
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000681:Amylases / Q000097:blood*; D001706:Biopsy / Q000009:adverse effects / Q000379:methods*; D044963:Biopsy, Fine-Needle / Q000009:adverse effects; D005260:Female; D006801:Humans; D008049:Lipase / Q000097:blood*; D008297:Male; D008875:Middle Aged; D010179:Pancreas / Q000293:injuries / Q000473:pathology; D010182:Pancreatic Diseases / Q000000981:diagnostic imaging / Q000473:pathology*; D010195:Pancreatitis / Q000201:enzymology* / Q000209:etiology; D011446:Prospective Studies; D012680:Sensitivity and Specificity; D018084:Ultrasonography, Interventional; D055815:Young Adult
chemical_list: D008049:Lipase; D000681:Amylases
keywords: Biopsia por aspiración con aguja fina; Endoscopic ultrasound; Enzimas pancreáticas; Fine needle aspiration biopsy; Pancreatic enzymes; Ultrasonido endoscópico