title: Label-free Imaging and Characterization of Cancer Cell Responses to Polymethoxyflavones Using Raman Microscopy.
abstract: We determined the cellular responses of human colon cancer HT29 and HCT116 cells to the treatments of nobiletin (NBT) and 5-demethylnobiletin (5DN) using Raman microscopy. Evaluation at both single cell and cell population levels revealed found that NBT induced more changes in the peak intensity of nucleic acid than 5DN, whereas 5DN induced more changes in the peak intensity of localized lipid than NBT. This result indicates the different modes of inhibitory action of these two PMFs against colon cancer cells. Between the two colon cancer cells tested, HCT116 cells were more sensitive to both PMFs than HT29 cells. The Raman data were generally in a good agreement with the flow cytometry data. Our results demonstrate that Raman microscopy is able to provide macromolecular information on cellular responses to anticancer treatments.
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences , Beijing 100193, People's Republic of China.
authors.forename: Jinkai
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Mathematics and Statistics, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Anna
authors.initials: A
authors.lastname: Liu
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Hang
authors.identifier: http://orcid.org/0000-0001-6194-2796
authors.initials: H
authors.lastname: Xiao
authors.affiliation: Department of Food Science, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Lili
authors.identifier: http://orcid.org/0000-0002-5046-9857
authors.initials: L
authors.lastname: He
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology*; D045744:Cell Line, Tumor; D002477:Cells / Q000166:cytology* / Q000187:drug effects*; D047309:Flavones / Q000494:pharmacology*; D006801:Humans; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: C000604601:5-demethylnobiletin; D000970:Antineoplastic Agents; D047309:Flavones; C008661:nobiletin
keywords: Raman microscopy; cell imaging; colon cancer cells; flow cytometry; polymethoxyflavones