title: MicroRNA-145 Mediates the Formation of Angiotensin II-Induced Murine Abdominal Aortic Aneurysm.
abstract: BACKGROUND
  MicroRNA-145 (miR-145) has been implicated in vascular smooth muscle cell differentiation, but the underlying mechanisms have not been fully understood, especially their role in abdominal aortic aneurysm (AAA) expansion. Here, we sought to explore and define the mechanisms of miR-145 function in the experimental AAA models in AngII-infused ApoE-/-mice.
  
  
  METHODS
  miR-145 was overexpressed in ApoE-/-mice via lentivirus infection, and then the incidence of AAA, maximum abdominal aortic diameter, elastin degradation and MMP2 activation were determined in AngII-infused ApoE-/-mice.
  
  
  RESULTS
  In vivo overexpression of miR-145 by lentivirus infection greatly decreased the incidence of AAA, maximum abdominal aortic diameter, and elastin degradation, accompanied with downregulation of MMP2 activation in AngII-infused ApoE-/-mice. Cell culture assays indicated that miR-145 inhibited AngII-induced upregulation of MMP2 gene expression. In contrast, deficiency of MMP2 abolished the effects of miR-145 on AngII-induced elastin and collagens degradations in ApoE-/-mice.
  
  
  CONCLUSIONS
  These data suggest that regulation of expression of miR-145 may be a potential therapeutic option for vascular disease progression such as AAA expansion.
authors.affiliation: School of Nursing, Gansu University of Chinese Medicine, Lanzhou 730000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Shenzhen Center for Chronic Disease Control, Shenzhen 518020, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Tumor Hospital of Jilin Province, Changchun 130021, China.
authors.forename: Xiaoou
authors.initials: X
authors.lastname: Li
authors.affiliation: Tumor Hospital of Jilin Province, Changchun 130021, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Liu
authors.affiliation: Tumor Hospital of Jilin Province, Changchun 130021, China.
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Yu
authors.affiliation: The First Hospital of Lanzhou University, Lanzhou 730000, China. Electronic address: yunlingtian7318@126.com.
authors.forename: Yunling
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000804:Angiotensin II / Q000009:adverse effects* / Q000494:pharmacology; D000818:Animals; D017544:Aortic Aneurysm, Abdominal / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology; D001057:Apolipoproteins E / Q000172:deficiency / Q000378:metabolism; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D004549:Elastin / Q000235:genetics / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D016086:Lentivirus; D020778:Matrix Metalloproteinase 2 / Q000096:biosynthesis* / Q000235:genetics; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D014161:Transduction, Genetic; D015854:Up-Regulation
chemical_list: D001057:Apolipoproteins E; C543499:MIRN145a microRNA, mouse; D035683:MicroRNAs; D000804:Angiotensin II; D003094:Collagen; D004549:Elastin; D020778:Matrix Metalloproteinase 2; C522362:Mmp2 protein, mouse
keywords: Aneurysm; MMP2; MicroRNA-145