title: Characterization and Genetic Analysis of Rice Mutantcrr1Exhibiting Compromised Non-host Resistance toPuccinia striiformisf. sp.tritici(Pst).
abstract: Wheat stripe rust, caused byPuccinia striiformisf. sp.tritici(Pst), is one of the most devastating diseases of wheat in China. Rapid change to virulence following release of resistant cultivars necessitates ongoing discovery and exploitation of new resistance resources. Considerable effort has been directed at non-host resistance (NHR) which is believed to be durable. In the present study we identified rice mutantcrr1(compromised resistance to rust 1) that exhibited compromised NHR toPst. Compared with wild type rice variety Nipponbare,crr1mutant displayed a threefold increase in penetration rate byPst, and enhanced hyphal growth. The pathogen also developed haustoria incrr1mesophyll cells, but failed to sporulate. The response to the adapted rice pathogenMagnaporthe oryzaewas unchanged incrr1relative to the wild type. Several defense-related genes involved in the SA- and JA-mediated defense pathways response and in phytoalexin synthesis (such asOsPR1a,OsLOX1, andOsCPS4) were more rapidly and strongly induced in infectedcrr1leaves than in the wild type, suggesting that other layers of defense are still in effect. Genetic analysis and mapping located the mutant loci at a region between markers ID14 and RM25792, which cover about 290 kb genome sequence on chromosome 10. Further fine mapping and cloning of the locus should provide further insights into NHR to rust fungi in rice, and may reveal new strategies for improving rust resistance in wheat.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Yuheng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Donghe
authors.initials: D
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Min
authors.initials: M
authors.lastname: Jiao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Gangming
authors.initials: G
authors.lastname: Zhan
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Life Science, Northwest A&F University Yangling, China.
authors.forename: Hongchang
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Shaanxi Rice Research Institute, Hanzhong Agricultural Science Institute Hanzhong, China.
authors.forename: Junyi
authors.initials: J
authors.lastname: Wang
authors.affiliation: Shaanxi Rice Research Institute, Hanzhong Agricultural Science Institute Hanzhong, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University Yangling, China.
authors.forename: Zhensheng
authors.initials: Z
authors.lastname: Kang
keywords: defense-related genes; genetic mapping; non-host resistance; rice mutant; wheat stripe rust