title: ReducedStaphylococcus aureusbiofilm formation in the presence of chitosan-coated iron oxide nanoparticles.
abstract: Staphylococcus aureuscan adhere to most foreign materials and form biofilm on the surface of medical devices. Biofilm infections are difficult to resolve. The goal of this in vitro study was to explore the use of chitosan-coated nanoparticles to prevent biofilm formation. For this purpose,S. aureuswas seeded in 96-well plates to incubate with chitosan-coated iron oxide nanoparticles in order to study the efficiency of biofilm formation inhibition. The biofilm bacteria count was determined using the spread plate method; biomass formation was measured using the crystal violet staining method. Confocal laser scanning microscopy and scanning electron microscopy were used to study the biofilm formation. The results showed decreased viable bacteria numbers and biomass formation when incubated with chitosan-coated iron oxide nanoparticles at all test concentrations. Confocal laser scanning microscopy showed increased dead bacteria and thinner biofilm when incubated with nanoparticles at a concentration of 500 µg/mL. Scanning electron microscopy revealed that chitosan-coated iron oxide nanoparticles inhibited biofilm formation in polystyrene plates. Future studies should be performed to study these nanoparticles for anti-infective use.
authors.affiliation: Department of Orthopedics, the Affiliated Hospital of Xu Zhou Medical University, Xu Zhou, Jiangsu; Department of Orthopedic Surgery, Shanghai sixth People's Hospital, Shanghai Jiao Tong University, Shanghai.
authors.forename: Si-Feng
authors.initials: SF
authors.lastname: Shi
authors.affiliation: School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, Shanghai.
authors.forename: Jing-Fu
authors.initials: JF
authors.lastname: Jia
authors.affiliation: Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai, People's Republic of China.
authors.forename: Xiao-Kui
authors.initials: XK
authors.lastname: Guo
authors.affiliation: School of Chemistry and Chemical Technology, Shanghai Jiao Tong University, Shanghai.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Zhao
authors.affiliation: Department of Orthopedic Surgery, Shanghai sixth People's Hospital, Shanghai Jiao Tong University, Shanghai.
authors.forename: De-Sheng
authors.initials: DS
authors.lastname: Chen
authors.affiliation: Department of Orthopedic Surgery, Shanghai sixth People's Hospital, Shanghai Jiao Tong University, Shanghai.
authors.forename: Yong-Yuan
authors.initials: YY
authors.lastname: Guo
authors.affiliation: Department of Orthopedic Surgery, Shanghai sixth People's Hospital, Shanghai Jiao Tong University, Shanghai.
authors.forename: Xian-Long
authors.initials: XL
authors.lastname: Zhang
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D048271:Chitosan / Q000737:chemistry*; D005290:Ferric Compounds / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D013211:Staphylococcus aureus / Q000187:drug effects*
chemical_list: D000900:Anti-Bacterial Agents; D005290:Ferric Compounds; C000499:ferric oxide; D048271:Chitosan
keywords: Staphylococcus aureus; biofilm; chitosan-coated iron oxide nanoparticles