title: Use of the APTIMA Combo 2 Assay and a Secondary Algorithm to Detect and Confirm Chlamydia trachomatis in Rectal-Only Infections.
abstract: We sought to confirm the results of 81 rectal specimens positive for Chlamydia trachomatis by the APTIMA Combo 2 assay among patients with concurrently collected negative genitourinary specimens. A total of 79 (97.5%) samples were confirmed by the APTIMA single target assay and/or sequencing of the C. trachomatis ompA gene.
authors.affiliation: From the *Provincial Laboratory for Public Health, Calgary, Alberta; †National Microbiology Laboratory, Public Health Agency of Canada; ‡Department of Medical Microbiology, Faculty of Health Sciences, University of Manitoba; §STI Centralized Services, Alberta Health Services; ¶Department of Medicine, University of Alberta; ∥Department of Medicine, **Department of Microbiology, Immunology and Infectious Diseases, University of Calgary; ††Provincial Laboratory for Public Health, Edmonton; and ‡‡Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, Alberta, Canada.
authors.forename: Kanti
authors.initials: K
authors.lastname: Pabbaraju
authors.forename: Sallene
authors.initials: S
authors.lastname: Wong
authors.forename: Kara
authors.initials: K
authors.lastname: Gill
authors.forename: Alberto
authors.initials: A
authors.lastname: Severini
authors.forename: Felicia
authors.initials: F
authors.lastname: Roy
authors.forename: Jennifer
authors.initials: J
authors.lastname: Gratrix
authors.forename: Ameeta E
authors.initials: AE
authors.lastname: Singh
authors.forename: Prenilla
authors.initials: P
authors.lastname: Naidu
authors.forename: Ron
authors.initials: R
authors.lastname: Read
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Drews
mesh_terms: D000465:Algorithms; D002690:Chlamydia Infections / Q000175:diagnosis* / Q000382:microbiology; D002692:Chlamydia trachomatis / Q000235:genetics / Q000302:isolation & purification*; D005260:Female; D006801:Humans; D008297:Male; D012007:Rectum / Q000382:microbiology*