title: Lactoferrin-coated polysaccharide nanoparticles based on chitosan hydrochloride/hyaluronic acid/PEG for treating brain glioma.
abstract: Curcuminoid (Cur) loaded polysaccharide nanoformulations with blood-brain barrier (BBB) penetration and brain targeting properties, based on hyaluronic acid (HA) and chitosan hydrochloride (CSH) (Lf-Cur-PSNPs) were developed as a novel delivery system for malignant glioma. Formulations were investigated for physicochemical characteristics, cytotoxicity, uptake, and BBB penetration. The results showed that LfM-Cur-PSNPs (concentration of Lf was 0.5mg/mL) were preferentially taken up by brain capillary endothelial cells than Cur-PSNPs at any time. After crossing the BBB, LfM-Cur-PSNPs remained largely intact and were more effective in targeting glioma cells (C6). In vivo imaging studies in mice exposed LfM-PSNPs could effectively permeate BBB and preferentially accumulate in the brain (2.39 times greater than PSNPs). Moreover, PSNPs were detected in brain up to 72h. This data indicates that Lf-Cur-PSNPs could effectively target and accumulate within the gliomas after enhanced permeation through BBB, thus should be further explored for their potential in CNS maliganancies.
authors.affiliation: Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Yurui
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China; Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Faisalabad, Pakistan.
authors.forename: Sajid
authors.initials: S
authors.lastname: Asghar
authors.affiliation: Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Affiliated Hospital of Weifang Medical University, Weifang 261031, PR China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Zhilin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China.
authors.forename: Qineng
authors.initials: Q
authors.lastname: Ping
authors.affiliation: Department of Pharmaceutics, State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing 210009, PR China. Electronic address: cpuyanyuxiao@163.com.
authors.forename: Yanyu
authors.initials: Y
authors.lastname: Xiao
mesh_terms: D000818:Animals; D001812:Blood-Brain Barrier; D001921:Brain; D045744:Cell Line, Tumor; D048271:Chitosan; D016503:Drug Delivery Systems; D005910:Glioma / Q000188:drug therapy*; D006820:Hyaluronic Acid; D007781:Lactoferrin; D051379:Mice; D053758:Nanoparticles; D011092:Polyethylene Glycols; D011134:Polysaccharides
chemical_list: D011134:Polysaccharides; D011092:Polyethylene Glycols; D006820:Hyaluronic Acid; D048271:Chitosan; D007781:Lactoferrin
keywords: Chitosan hydrochloride; Curcuminoid; Glioma targeting; Hyaluronic acid; Lactoferrin; Polysaccharide nanoparticles