title: IL-6 Promotes Isletβ-Cell Dysfunction in Rat Collagen-Induced Arthritis.
abstract: The aim of this study was to explore the possible mechanism of rheumatoid arthritis- (RA-) related abnormal glucose metabolism. The model of collagen-induced arthritis (CIA) was established by intradermal injection of type II collagen into Wistar rats; complete Freund's adjuvant injections were used as the control group. Fasting plasma glucose (FBG) was measured by the glucose oxidase method. Fasting insulin (FIns) and the expressions of IL-6 were detected by ELISA. Islet caspase-3 was examined by immunohistochemistry. On day 17 after immunization, FBG of the CIA group showed an elevated FBG value compared with the control group. Meanwhile, the FIns of group CIA was lower when compared with the control group. Interestingly, the inflammatory cytokine IL-6 expression was significantly increased when compared with the control group. As expected, the abnormal glucose metabolism was accompanied by the increased IL-6 expression. Furthermore, in line with the upregulated IL-6 expression, the apoptosis related enzyme caspase-3 was also markedly increased. These data showed that the elevated FBG in CIA may be associated with the reduced FIns level secondary to the overapoptosis of pancreas islet cells induced by IL-6.
authors.affiliation: Department of Rheumatology, Jiangxi Provincial People's Hospital, Nanchang 330006, China.
authors.forename: Huan
authors.identifier: 0000-0002-7146-3569
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Critical Care Medicine, The First Affiliated Hospital of Xiamen University, Xiamen 361003, China.
authors.forename: Yaogui
authors.initials: Y
authors.lastname: Ning
authors.affiliation: Department of Rheumatology, Jiangxi Provincial People's Hospital, Nanchang 330006, China.
authors.forename: Haotong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Department of Rheumatology, Jiangxi Provincial People's Hospital, Nanchang 330006, China.
authors.forename: Youlian
authors.identifier: 0000-0003-3363-5486
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001169:Arthritis, Experimental / Q000378:metabolism*; D001172:Arthritis, Rheumatoid / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism*; D053148:Caspase 3 / Q000378:metabolism*; D004195:Disease Models, Animal; D005215:Fasting; D005260:Female; D007150:Immunohistochemistry; D007328:Insulin / Q000378:metabolism*; D050417:Insulin-Secreting Cells / Q000378:metabolism*; D015850:Interleukin-6 / Q000378:metabolism*; D007515:Islets of Langerhans / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001786:Blood Glucose; D007328:Insulin; D015850:Interleukin-6; C505410:Casp3 protein, rat; D053148:Caspase 3