title: A novel electrochemical biosensor based on Fe3O4nanoparticles-polyvinyl alcohol composite for sensitive detection of glucose.
abstract: In this research, a new electrochemical biosensor was constructed for the glucose detection. Iron oxide nanoparticles (Fe3O4) were synthesized through co-precipitation method. Polyvinyl alcohol-Fe3O4nanocomposite was prepared by dispersing synthesized nanoparticles in the polyvinyl alcohol (PVA) solution. Glucose oxidase (GOx) was immobilized on the PVA-Fe3O4nanocomposite via physical adsorption. The mixture of PVA, Fe3O4nanoparticles and GOx was drop cast on a tin (Sn) electrode surface (GOx/PVA-Fe3O4/Sn). The Fe3O4nanoparticles were characterized by X-ray diffraction (XRD). Also, Fourier transform infrared (FTIR) spectroscopy and field emission scanning electron microscopy (FE-SEM) techniques were utilized to evaluate the PVA-Fe3O4and GOx/PVA-Fe3O4nanocomposites. The electrochemical performance of the modified biosensor was investigated using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Presence of Fe3O4nanoparticles in the PVA matrix enhanced the electron transfer between enzyme and electrode surface and the immobilized GOx showed excellent catalytic characteristic toward glucose. The GOx/PVA-Fe3O4/Sn bioelectrode could measure glucose in the range from 5 × 10-3to 30 mM with a sensitivity of 9.36 μA mM-1and exhibited a lower detection limit of 8 μM at a signal-to-noise ratio of 3. The value of Michaelis-Menten constant (KM) was calculated as 1.42 mM. The modified biosensor also has good anti-interfering ability during the glucose detection, fast response (10 s), good reproducibility and satisfactory stability. Finally, the results demonstrated that the GOx/PVA-Fe3O4/Sn bioelectrode is promising in biosensor construction.
authors.affiliation: Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Niuosha
authors.initials: N
authors.lastname: Sanaeifar
authors.affiliation: Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rabiee
authors.affiliation: Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Abdolrahim
authors.affiliation: Biomaterial Group, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran; Marquette University School of Dentistry, Milwaukee, WI 53233, USA. Electronic address: mohammadreza.tahriri@marquette.edu.
authors.forename: Mohammadreza
authors.initials: M
authors.lastname: Tahriri
authors.affiliation: Electrical and Computer Engineering Department, North Carolina State University, Raleigh, NC 27606, USA.
authors.forename: Daryoosh
authors.initials: D
authors.lastname: Vashaee
authors.affiliation: Marquette University School of Dentistry, Milwaukee, WI 53233, USA; Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK. Electronic address: lobat.tayebi@marquette.edu.
authors.forename: Lobat
authors.initials: L
authors.lastname: Tayebi
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D058266:Dielectric Spectroscopy; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry*; D005947:Glucose / Q000032:analysis*; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D059629:Signal-To-Noise Ratio; D014961:X-Ray Diffraction
chemical_list: D004800:Enzymes, Immobilized; D005290:Ferric Compounds; C000499:ferric oxide; D011142:Polyvinyl Alcohol; D005949:Glucose Oxidase; D005947:Glucose
keywords: Biosensor; Electrochemical; Electrode; Fe(3)O(4) nanoparticles; Glucose; Poly(vinyl alcohol)