title: Novel 99mTc-Radiolabeled Anionic Linear Globular PEG-Based Dendrimer-Chlorambucil: Non-Invasive Method for In-Vivo Biodistribution.
abstract: Chlorambucil (CBL) is an alkylating agent, which widely use in the treatment of various types of tumors. The main purpose of this study is to evaluate the in-vivo biodistribution of CBL conjugated to the anionic dendrimer, which has a great ability to labeled with99mTc through binding to carboxylate terminate groups. Whole body scans were used to analyze the percentage of the injected dose in different times. Radiochemical purity (RCP) and in-vivo biodistribution were also calculated with the SPECT/CT instrument. Our study proposes a new method for RCP determination and shows that this carrier is a promising agent to complex with the99mTc and biological assessment.
authors.affiliation: Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyedeh Masoomeh
authors.initials: SM
authors.lastname: Ghoreishi
authors.affiliation: Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Khalaj
authors.affiliation: Department of Nuclear Medicine, Cardiovascular Intervention Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Bitarafan-Rajabi
authors.affiliation: Department of Nuclear Medicine, Cardiovascular Intervention Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Amir Darbandi
authors.initials: AD
authors.lastname: Azar
authors.affiliation: Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehdi Shafiee
authors.initials: MS
authors.lastname: Ardestani
authors.affiliation: Faculty of Pharmacy, Department of Radiopharmacy, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Artin
authors.initials: A
authors.lastname: Assadi
mesh_terms: D000818:Animals; D000838:Anions / Q000737:chemistry / Q000378:metabolism*; D002699:Chlorambucil / Q000737:chemistry / Q000378:metabolism*; D050091:Dendrimers / Q000737:chemistry / Q000378:metabolism*; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D011092:Polyethylene Glycols / Q000737:chemistry* / Q000378:metabolism; D011817:Rabbits; D013667:Technetium / Q000737:chemistry / Q000378:metabolism*; D014018:Tissue Distribution / Q000187:drug effects*; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods
chemical_list: D000838:Anions; D050091:Dendrimers; D002699:Chlorambucil; D011092:Polyethylene Glycols; D013667:Technetium