title: Backbone chemical shift assignments and secondary structure analysis of the U1 protein from the Bas-Congo virus.
abstract: The Bas-Congo virus (BASV) is the first rhabdovirus associated with a human outbreak of acute hemorrhagic fever. The single-stranded, negative-sense RNA genome of BASV contains the five core genes present in all rhabdoviral genomes plus an additional three genes, annotated U1, U2, and U3, with weak (<21%) sequence similarity only to a handful of genes observed in a few other rhabdoviral genomes. The function of the rhabdoviral U proteins is unknown, but, they are hypothesized to play a role in viral infection or replication. To better understand this unique family of proteins, a construct containing residues 27-203 of the 216-residue U1 protein (BASV-U1*) was prepared. By collecting data in 0.5 M urea it was possible to eliminate transient association enough to enable the assignment of most of the observable1HN,1Hα,15N,13Cα,13Cβ, and13C´ chemical shifts for BASV-U1* that will provide a foundation to study its solution properties. The analyses of these chemical shifts along with15N-edited NOESY data enabled the identification of the elements of secondary structure present in BASV-U1*.
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA. garry.buchko@pnnl.gov.|Earth and Biological Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, 99352, USA. garry.buchko@pnnl.gov.
authors.forename: Garry W
authors.initials: GW
authors.lastname: Buchko
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Beryllium, Bainbridge Island, WA, 97331, USA.|Nurix Inc., San Francisco, CA, 94158, USA.
authors.forename: Matthew C
authors.initials: MC
authors.lastname: Clifton
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Beryllium, Bainbridge Island, WA, 97331, USA.
authors.forename: Ellen G
authors.initials: EG
authors.lastname: Wallace
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Beryllium, Bainbridge Island, WA, 97331, USA.
authors.forename: Kateri A
authors.initials: KA
authors.lastname: Atkins
authors.affiliation: Seattle Structural Genomics Center for Infectious Disease, Seattle, USA.|Center for Infectious Disease Research, 307 Westlake Ave. N., Seattle, WA, 98109, USA.|Department of Global Health, University of Washington, Seattle, WA, 98165, USA.|Department of Biomedical Informatics and Health Education, University of Washington, Seattle, WA, 98195, USA.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Myler
mesh_terms: D019906:Nuclear Magnetic Resonance, Biomolecular; D017433:Protein Structure, Secondary; D012209:Rhabdoviridae; D014764:Viral Proteins / Q000737:chemistry* / Q000378:metabolism
chemical_list: D014764:Viral Proteins
keywords: Chemical shift index; Drug design; Rhabdovirus; Viral hemorrhagic fever