title: Biofunctionalized Nanostructured Zirconia for Biomedical Application: A Smart Approach for Oral Cancer Detection.
abstract: Results of the studies are reported relating to application of the silanized nanostructured zirconia, electrophoretically deposited onto indium tin oxide (ITO) coated glass for covalent immobilization of the monoclonal antibodies (anti-CYFRA-21-1). This biosensing platform has been utilized for a simple, efficient, noninvasive, and label-free detection of oral cancer via cyclic voltammetry technique. The results of electrochemical response studies conducted on bovine serum albumin (BSA)/anti-CYFRA-21-1/3-aminopropyl triethoxy silane (APTES)/ZrO2/ITO immunoelectrode reveal that this immunoelectrode can be used to measure CYFRA-21-1 (oral cancer biomarker) concentration in saliva samples, with a high sensitivity of 2.2 mA mL ng-1, a linear detection range of 2-16 ng mL-1, and stability of six weeks. The results of these studies have been validated via enzyme-linked immunosorbent assay.
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Suveen
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Sachchidanand
authors.initials: S
authors.lastname: Tiwari
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Saurabh
authors.initials: S
authors.lastname: Srivastava
authors.affiliation: Department of Physics and Astrophysics University of Delhi New Delhi 110007 India.
authors.forename: Manish
authors.initials: M
authors.lastname: Srivastava
authors.affiliation: Rajiv Gandhi Cancer Institute and Research Centre Rohini New Delhi 110085 India.
authors.forename: Birendra Kumar
authors.initials: BK
authors.lastname: Yadav
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Saroj
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Department of Chemical and Environmental Engineering University of California Riverside CA 92521 USA.
authors.forename: Thien Toan
authors.initials: TT
authors.lastname: Tran
authors.affiliation: Rajiv Gandhi Cancer Institute and Research Centre Rohini New Delhi 110085 India.
authors.forename: Ajay Kumar
authors.initials: AK
authors.lastname: Dewan
authors.affiliation: Department of Chemical and Environmental Engineering University of California Riverside CA 92521 USA.
authors.forename: Ashok
authors.initials: A
authors.lastname: Mulchandani
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Jai Gopal
authors.initials: JG
authors.lastname: Sharma
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Sagar
authors.initials: S
authors.lastname: Maji
authors.affiliation: Nanobioelectronics Laboratory Department of Biotechnology Delhi Technological University New Delhi 110042 India.
authors.forename: Bansi Dhar
authors.initials: BD
authors.lastname: Malhotra
keywords: CYFRA‐21‐1; biosensors; noninvasive detection; oral cancer; zirconia