title: Proteome Changes inPenicillium expansumGrown in a Medium Derived from Host Plant.
abstract: Penicillium expansumcauses blue mold rot, a prevalent postharvest disease of pome fruit, and is also the main producer of the patulin. However, knowledge on the molecular mechanisms involved in this pathogen-host interaction remains largely unknown. In this work, a two-dimensional gel electrophoresis-based proteomic approach was applied to probe changes inP. expansum3.3703 cultivated in apple juice medium, which was used to mimic the in planta condition. The results showed that the pH value and reducing sugar content in the apple juice medium decreased whereas the patulin content increased with the growing ofP. expansum. A total of 28 protein spots that were up-regulated inP. expansumwhen grown in apple juice medium were identified. Functional categorization revealed that the identified proteins were mainly related to carbohydrate metabolism, secondary metabolism, protein biosynthesis or degradation, and redox homeostasis. Remarkably, several induced proteins, including glucose dehydrogenase, galactose oxidase, and FAD-binding monooxygenase, which might be responsible for the observed medium acidification and patulin production, were also detected. Overall, the experimental results provide a comprehensive interpretation of the physiological and proteomic responses ofP. expansumto the host plant environment, and future functional characterization of the identified proteins will deepen our understanding of fungi-host interactions.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Xiaoshuang
authors.initials: X
authors.lastname: Xia
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, P.R. China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, P.R. China.
authors.forename: Peiwu
authors.initials: P
authors.lastname: Li
mesh_terms: D018533:Biomass; D019295:Computational Biology / Q000379:methods; D003470:Culture Media; D054884:Host-Pathogen Interactions; D053858:Metabolic Networks and Pathways; D010407:Penicillium / Q000254:growth & development / Q000378:metabolism*; D010935:Plant Diseases / Q000382:microbiology; D010944:Plants / Q000382:microbiology*; D020543:Proteome; D040901:Proteomics / Q000379:methods
chemical_list: D003470:Culture Media; D020543:Proteome
keywords: Penicillium expansum; apple juice medium; fungi-host interaction; proteomics