title: [Interaction of DNAJB13 with HK1 in mouse].
abstract: OBJECTIVE
  To investigate the presence of interactions between DNAJB13 and HK1.
  
  
  METHODS
  The open reading frame of Dnajb13 gene was amplified from mouse testis cDNA by PCR. The PCR products were then inserted into pGEX-4T-1 vector after double digestion and identified by sequencing. The recombinant plasmids were transformated into competent DH5a cells, and the fusion protein was expressed with IPTG induction. SDS-PAGE Coomassie brilliant blue staining and Western blot analysis were used to detect the fusion protein expression. The protein precipitated by GST-DNAJB13 in GST pull down assay was detected by Western blotting.
  
  
  RESULTS
  The recombinant plasmid pGEX-4T-1-Dnajb13 was successfully constructed and verified. E.coli transformed with the recombinant plasmid expressed abundant fusion protein. GST pull down assay showed interactions between DNAJB13 and HK1.
  
  
  CONCLUSIONS
  DNAJB13 interacts with HK1 in mouse testis and probably participates in spermatogenesis and the regulation of sperm motility.
authors.affiliation: Institute of Reproductive and Stem Cell Engineering, Central South University, Changsha 410078, China. E-mail: 1612492768@qq.com.
authors.forename: Meng-Yue
authors.initials: MY
authors.lastname: Yang
authors.forename: Zi-Wei
authors.initials: ZW
authors.lastname: Xiong
authors.forename: Wei-Na
authors.initials: WN
authors.lastname: Li
authors.forename: Miao-Miao
authors.initials: MM
authors.lastname: Jia
authors.forename: Gang
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D051017:Apoptosis Regulatory Proteins; D015153:Blotting, Western; D018076:DNA, Complementary; D004591:Electrophoresis, Polyacrylamide Gel; D004926:Escherichia coli; D050956:HSP40 Heat-Shock Proteins / Q000502:physiology*; D006593:Hexokinase / Q000502:physiology*; D051379:Mice; D018832:Molecular Chaperones; D010957:Plasmids; D016133:Polymerase Chain Reaction; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; D013081:Sperm Motility; D013091:Spermatogenesis / Q000502:physiology*
chemical_list: D051017:Apoptosis Regulatory Proteins; D018076:DNA, Complementary; C531170:DNAJB13 protein, mouse; D050956:HSP40 Heat-Shock Proteins; D018832:Molecular Chaperones; D011993:Recombinant Fusion Proteins; D011994:Recombinant Proteins; C515658:HK1 protein, mouse; D006593:Hexokinase