title: Transcriptomic information from Pacific white shrimp (Litopenaeus vannamei) ovary and eyestalk, and expression patterns for genes putatively involved in the reproductive process.
abstract: The increased use of massive sequencing technologies has enabled the identification of several genes known to be involved in different mechanisms associated with reproduction that so far have only been studied in vertebrates and other model invertebrate species. In order to further investigate the genes involved in Litopenaeus vannamei reproduction, cDNA and SSH libraries derived from female eyestalk and gonad were produced, allowing the identification of expressed sequences tags (ESTs) that potentially have a role in the regulation of gonadal maturation. In the present study, different transcripts involved in reproduction were identified and a number of them were characterized as full-length. These transcripts were evaluated in males and females in order to establish their tissue expression profiles during developmental stages (juvenile, subadult and adult), and in the case of females, their possible association with gonad maturation was assessed through expression analysis of vitellogenin. The results indicated that the expression of vitellogenin receptor (vtgr) and minichromosome maintenance (mcm) family members in the female gonad suggest an important role during previtellogenesis. Additionally, the expression profiles of genes such as famet, igfbp and gpcr in brain tissues suggest an interaction between the insulin/insulin-like growth factor signaling pathway (IIS) and methyl farnesoate (MF) biosynthesis for control of reproduction. Furthermore, the specific expression pattern of farnesoic acid O-methyltransferase suggests that final synthesis of MF is carried out in different target tissues, where it is regulated by esterase enzymes under a tissue-specific hormonal control. Finally, the presence of a vertebrate type steroid receptor in hepatopancreas and intestine besides being highly expressed in female gonads, suggest a role of that receptor during sexual maturation.
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, La Paz, Baja California Sur 23096, Mexico. Electronic address: cventura@cibnor.mx.
authors.forename: Claudia
authors.initials: C
authors.lastname: Ventura-López
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, La Paz, Baja California Sur 23096, Mexico. Electronic address: pgalindo@pg.cibnor.mx.
authors.forename: Pavel E
authors.initials: PE
authors.lastname: Galindo-Torres
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, La Paz, Baja California Sur 23096, Mexico. Electronic address: farcos04@cibnor.mx.
authors.forename: Fabiola G
authors.initials: FG
authors.lastname: Arcos
authors.affiliation: Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Carretera Ensenada-Tijuana No. 3918, Zona Playitas, Ensenada, Baja California CP 22860, Mexico. Electronic address: cgalindo@cicese.mx.
authors.forename: Clara
authors.initials: C
authors.lastname: Galindo-Sánchez
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, La Paz, Baja California Sur 23096, Mexico. Electronic address: iracotta@cibnor.mx.
authors.forename: Ilie S
authors.initials: IS
authors.lastname: Racotta
authors.affiliation: Consejo Nacional de Ciencia y Tecnología (CONACYT) - Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional 195, Col. Playa Palo de Santa Rita, La Paz, Baja California Sur C.P. 23096, Mexico. Electronic address: cescobedo@cibnor.mx.
authors.forename: Cristina
authors.initials: C
authors.lastname: Escobedo-Fregoso
authors.affiliation: Consejo Nacional de Ciencia y Tecnología (CONACYT) - Centro de Investigación en Alimentación y Desarrollo A.C. (CIAD) Unidad Mazatlán, Av. Sábalo-Cerritos s/n. Estero del Yugo, Mazatlán, Sinaloa 82000, Mexico. Electronic address: raul.llera@ciad.mx.
authors.forename: Raúl
authors.initials: R
authors.lastname: Llera-Herrera
authors.affiliation: Centro de Investigaciones Biológicas del Noroeste, S.C. (CIBNOR), Av. Instituto Politécnico Nacional No.195, Col. Playa Palo de Santa Rita, La Paz, Baja California Sur 23096, Mexico. Electronic address: aibarra@cibnor.mx.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Ibarra
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis*; D018076:DNA, Complementary / Q000378:metabolism; D004527:Egg Proteins / Q000235:genetics / Q000378:metabolism; D020224:Expressed Sequence Tags; D005123:Eye / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D008297:Male; D010053:Ovary / Q000378:metabolism*; D033561:Penaeidae / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D011956:Receptors, Cell Surface / Q000235:genetics / Q000378:metabolism; D012098:Reproduction / Q000502:physiology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: D015415:Biomarkers; D018076:DNA, Complementary; D004527:Egg Proteins; D012333:RNA, Messenger; D011956:Receptors, Cell Surface; C030474:vitellogenin receptor
keywords: Litopenaeus vannamei; Reproduction; Tissues-genders-temporal-expression