title: Glycogen metabolism in the glucose-sensing and supply-driven β-cell.
abstract: Glycogen metabolism in β-cells may affect downstream metabolic pathways controlling insulin release. We examined glycogen metabolism in human islets and in the rodent-derived INS-1 832/13 β-cells and found them to express the same isoforms of key enzymes required for glycogen metabolism. Our findings indicate that glycogenesis is insulin-independent but influenced by extracellular glucose concentrations. Levels of glycogen synthase decrease with increasing glucose concentrations, paralleling accumulation of glycogen. We did not find cAMP-elicited glycogenolysis and insulin secretion to be causally related. In conclusion, our results reveal regulated glycogen metabolism in human islets and insulin-secreting cells. Whether glycogen metabolism affects insulin secretion under physiological conditions remains to be determined.
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Lotta E
authors.initials: LE
authors.lastname: Andersson
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Lisa M
authors.initials: LM
authors.lastname: Nicholas
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Karin
authors.initials: K
authors.lastname: Filipsson
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Jiangming
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Anya
authors.initials: A
authors.lastname: Medina
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Al-Majdoub
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Malin
authors.initials: M
authors.lastname: Fex
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.
authors.forename: Hindrik
authors.initials: H
authors.lastname: Mulder
authors.affiliation: Department of Clinical Sciences, Unit of Molecular Metabolism, Lund University Diabetes Centre, CRC, Malmö, Sweden.|Department of Chemistry, Centre for Analysis and Synthesis, Lund University, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Spégel
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D002460:Cell Line; D005576:Colforsin / Q000494:pharmacology; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology*; D006003:Glycogen / Q000378:metabolism*; D006006:Glycogen Synthase / Q000378:metabolism; D006801:Humans; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D050417:Insulin-Secreting Cells / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D007328:Insulin; D005576:Colforsin; D006003:Glycogen; D006006:Glycogen Synthase; D005947:Glucose; D015056:1-Methyl-3-isobutylxanthine
keywords: INS-1 832/13; human islets; insulin secretion