title: Plasmonic Nanosensors for the Determination of Drug Effectiveness on Membrane Receptors.
abstract: We demonstrate the potential of the NanoSPR (nanoscale surface plasmon resonance sensors) method as a simple and cheap tool for the quantitative study of membrane protein-protein interactions. We use NanoSPR to determine the effectiveness of two potential drug candidates that inhibit the protein complex formation between FtsA and ZipA at initial stages of bacterial division. As the NanoSPR method relies on individual gold nanorods as sensing elements, there is no need for fluorescent labels or organic cosolvents, and it provides intrinsically high statistics. NanoSPR could become a powerful tool in drug development, drug delivery, and membrane studies.
authors.affiliation: Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.
authors.forename: Rubén
authors.identifier: 0000-0002-9863-0443
authors.initials: R
authors.lastname: Ahijado-Guzmán
authors.affiliation: Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Menten
authors.affiliation: Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.|Graduate School Materials Science in Mainz , Staudingerweg 9, D-55128 Mainz, Germany.
authors.forename: Janak
authors.initials: J
authors.lastname: Prasad
authors.affiliation: Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.
authors.forename: Christina
authors.initials: C
authors.lastname: Lambertz
authors.affiliation: Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Cientı́ficas , c/Ramiro de Maeztu 9, 28040 Madrid, Spain.
authors.forename: Germán
authors.initials: G
authors.lastname: Rivas
authors.affiliation: Institute of Physical Chemistry, University of Mainz , Duesbergweg 10-14, D-55128 Mainz, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Sönnichsen
mesh_terms: D001426:Bacterial Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D004926:Escherichia coli; D029968:Escherichia coli Proteins; D049329:Nanostructures; D011485:Protein Binding; D020349:Surface Plasmon Resonance
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D003598:Cytoskeletal Proteins; D029968:Escherichia coli Proteins
keywords: FtsZ inhibitors; NanoSPR; drug effectiveness; optical dark-field spectroscopy; plasmonic nanosensors; protein−membrane receptor interactions