title: pH-Dependent Fluorescent Probe That Can Be Tuned for Cysteine or Homocysteine.
abstract: The very close structural similarities between cysteine and homocysteine present a great challenge to achieve their selective detection using regular fluorescent probes, limiting the biological and pathological studies of these two amino thiols. A coumarin-based fluorescent probe was designed featuring pH-promoted distinct turn-on followed by ratiometric fluorescence responses for Cys and turn-on fluorescence response for Hcy through two different reaction paths. These specific responses demonstrate the activity differences between Cys and Hcy qualitatively for the first time. The probe could also be used for Cys and Hcy imaging in living cells.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Yue
authors.forename: Fangjun
authors.initials: F
authors.lastname: Huo
authors.forename: Xiaoqi
authors.initials: X
authors.lastname: Li
authors.forename: Ying
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Department of Chemistry and Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University , Shanghai 200433, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Yi
authors.affiliation: Department of Chemistry, Portland State University , Portland, Oregon 97201, United States.
authors.forename: James
authors.initials: J
authors.lastname: Salamanca
authors.affiliation: Department of Chemistry, Portland State University , Portland, Oregon 97201, United States.
authors.forename: Jorge O
authors.initials: JO
authors.lastname: Escobedo
authors.affiliation: Department of Chemistry, Portland State University , Portland, Oregon 97201, United States.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Strongin
authors.forename: Caixia
authors.identifier: 0000-0001-5548-6333
authors.initials: C
authors.lastname: Yin
mesh_terms: D015374:Biosensing Techniques; D058948:Cell Tracking; D003374:Coumarins / Q000737:chemistry*; D003500:Cyclization; D003545:Cysteine / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D056945:Hep G2 Cells; D006710:Homocysteine / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D012996:Solutions; D013050:Spectrometry, Fluorescence / Q000379:methods; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D003374:Coumarins; D005456:Fluorescent Dyes; D012996:Solutions; D013438:Sulfhydryl Compounds; D006710:Homocysteine; C030123:coumarin; D003545:Cysteine