title: Identification of phenolic antioxidants and bioactives of pomegranate seeds following juice extraction using HPLC-DAD-ESI-MSn.
abstract: Phenolics from free and hydrolyzed fractions of pomegranate juice (PJ) and seeds (PS) were evaluated. In general, total phenolic contents and scavenging of ABTS+, DPPH and hydroxyl radicals, as well as metal chelation of the soluble fraction from PS, were higher than those for PJ. Insoluble-bound phenolics from PS accounted for up to 27% of total scavenging capacity (free+esterified+insoluble-bound). Phenolic acids (13), monomeric flavonoids (8), hydrolysable tannins (12), proanthocyanidin (1) and anthocyanins (12) were tentatively characterized using HPLC-DAD-ESI-MSn. Several compounds were identified for the first time in PJ or PS. The inhibition of DNA damage (induced by hydroxyl and peroxyl radicals), copper-induced LDL-cholesterol peroxidation, as well as alpha-glucosidase and lipase activities were demonstrated, therefore supporting the potential exploitation of PJ and PS as sources of bioactive compounds.
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada.
authors.forename: Priyatharini
authors.initials: P
authors.lastname: Ambigaipalan
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada; Department of Agri-Food Industry, Food & Nutrition, "Luiz de Queiroz" College of Agriculture, University of São Paulo, Av. Pádua Dias 11, P.O. Box 9, CEP 13418-900 Piracicaba, SP, Brazil.
authors.forename: Adriano Costa
authors.initials: AC
authors.lastname: de Camargo
authors.affiliation: Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B 3X9, Canada. Electronic address: fshahidi@mun.ca.
authors.forename: Fereidoon
authors.initials: F
authors.lastname: Shahidi
mesh_terms: D000872:Anthocyanins / Q000032:analysis; D000975:Antioxidants / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D005419:Flavonoids / Q000032:analysis*; D047348:Hydrolyzable Tannins / Q000032:analysis; D029561:Lythraceae / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry*; D000520:alpha-Glucosidases / Q000032:analysis
chemical_list: D000872:Anthocyanins; D000975:Antioxidants; D005419:Flavonoids; D047348:Hydrolyzable Tannins; D010636:Phenols; D010936:Plant Extracts; D000520:alpha-Glucosidases
keywords: Alpha-glucosidase; Antioxidant activity; DNA damage; Human LDL-cholesterol peroxidation; Lipase; Punica granatum L.