title: Virus-like particle vaccine by intranasal vaccination elicits protective immunity against respiratory syncytial viral infection in mice.
abstract: Respiratory syncytial virus (RSV) is a leading cause of lower respiratory infection in infants and children, but there is still no licensed vaccine available. In this report, we developed virus-like particle (VLP) vaccines based on the Bac-to-Bac baculovirus expression system, consisting of an influenza virus matrix (M1) protein and the RSV fusion protein (F) or glycoprotein (G). These RSV VLPs were identified by western blot analysis and electron microscopy. Female BALB/c mice immunized intranasally (i.n.) with RSV-F VLPs, RSV-G VLPs, or both showed viral-specific antibody responses against RSV. Total IgG, IgG1, IgG2a, and mucosal IgA were detected in mice with RSV-F plus RSV-G VLPs, revealing potent cellular and mucosal immune responses. Moreover, we found that these mixed RSV VLPs conferred enhanced protection against live RSV challenges, showing significant decreases in lung viral replication and obvious attenuation of histopathological changes associated with viral infections. These results demonstrate that RSV-F plus RSV-G VLPs by intranasal vaccination is a promising vaccine candidate that warrants further evaluation using cotton rat and primate models.
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Cai
authors.affiliation: Beijing 307 Hospital, Affiliated to Academy of Medical Sciences, Beijing 100071, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Gu
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Sujing
authors.initials: S
authors.lastname: Sun
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Yueqiang
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Chengcai
authors.initials: C
authors.lastname: Lai
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Keyu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Yang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Xing
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Peirui
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Zhaohai
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Shaogeng
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Beijing 307 Hospital, Affiliated to Academy of Medical Sciences, Beijing 100071, China.
authors.forename: Shubing
authors.initials: S
authors.lastname: Liu
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China.
authors.forename: Yigang
authors.initials: Y
authors.lastname: Tong
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China ypenghuiamms@hotmail.com xiliangw@126.com.
authors.forename: Xiliang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Beijing Institute of Microbiology and Epidemiology, State Key Laboratory of Pathogen and Biosecurity, Beijing 100071, China ypenghuiamms@hotmail.com xiliangw@126.com.|Beijing 302 Hospital, Beijing 100039, China.
authors.forename: Penghui
authors.initials: P
authors.lastname: Yang
mesh_terms: D000281:Administration, Intranasal; D000818:Animals; D005260:Female; D007111:Immunity, Cellular; D018928:Immunity, Mucosal; D051379:Mice; D008807:Mice, Inbred BALB C; D018357:Respiratory Syncytial Virus Infections / Q000276:immunology / Q000517:prevention & control*; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage* / Q000276:immunology
chemical_list: D058425:Vaccines, Virus-Like Particle
keywords: fusion protion; glycoprotein; intranasal vaccination; respiratory syncytial virus; virus-like particles