title: Pilot production of ulvans from Ulva sp. and their effects on hyaluronan and collagen production in cultured dermal fibroblasts.
abstract: Ulvans from Ulva sp. were tested for their potential cosmetic properties on human dermal fibroblasts. The crude ulvans (ULVAN-01, 57kDa), extracted using a patented acid- and solvent-free process, were subjected to depolymerization using ion exchange resin to obtain a low molecular weight ulvan (ULVAN-DEP, 4kDa). The biochemical characterization and UHPLC-HRMS analyses of these extracted ulvans showed that they were of high purity and predominantly composed of a repeated ulvanobiouronic acid disaccharide. Fibroblast proliferation, as well as hyaluronan and collagen release were assessed, demonstrating that ULVAN-01 reduced fibroblast proliferation rate while ULVAN-DEP had no significant effect. Both ulvans were ineffective to induce collagen production but induced a significant increase in hyaluronan production, with a strong influence of the molecular weight. Thus, crude and depolymerized ulvans had different metabolic activities on dermal fibroblasts, which makes them promising to envisage further development in the skin care field.
authors.affiliation: Université de La Rochelle, UMR CNRS 7266, LIENSS, Equipe Approches Moléculaires Environnement-Santé, Département de Biotechnologies, Avenue Michel Crépeau, 17042 La Rochelle, France; SEPROSYS, Séparations, Procédés, Systèmes, 12 Rue Marie-Aline Dusseau, 17000 Rochelle, France.
authors.forename: Amandine
authors.initials: A
authors.lastname: Adrien
authors.affiliation: Université de La Rochelle, UMR CNRS 7266, LIENSS, Equipe Approches Moléculaires Environnement-Santé, Département de Biotechnologies, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Antoine
authors.initials: A
authors.lastname: Bonnet
authors.affiliation: SEPROSYS, Séparations, Procédés, Systèmes, 12 Rue Marie-Aline Dusseau, 17000 Rochelle, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Dufour
authors.affiliation: SEPROSYS, Séparations, Procédés, Systèmes, 12 Rue Marie-Aline Dusseau, 17000 Rochelle, France.
authors.forename: Stanislas
authors.initials: S
authors.lastname: Baudouin
authors.affiliation: Université de La Rochelle, UMR CNRS 7266, LIENSS, Equipe Approches Moléculaires Environnement-Santé, Département de Biotechnologies, Avenue Michel Crépeau, 17042 La Rochelle, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Maugard
authors.affiliation: Université de La Rochelle, UMR CNRS 7266, LIENSS, Equipe Approches Moléculaires Environnement-Santé, Département de Biotechnologies, Avenue Michel Crépeau, 17042 La Rochelle, France. Electronic address: nicolas.bridiau@univ-lr.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bridiau
mesh_terms: D002478:Cells, Cultured; D003094:Collagen / Q000378:metabolism*; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D006820:Hyaluronic Acid / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D011134:Polysaccharides / Q000302:isolation & purification / Q000494:pharmacology*; D044445:Ulva / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D011134:Polysaccharides; C571831:ulvan; D006820:Hyaluronic Acid; D003094:Collagen
keywords: Collagen; Green seaweed; Human dermal fibroblast; Hyaluronan; Skin care; Ulvan