title: Nitric Oxide Overproduction in TomatoshrMutant Shifts Metabolic Profiles and Suppresses Fruit Growth and Ripening.
abstract: Nitric oxide (NO) plays a pivotal role in growth and disease resistance in plants. It also acts as a secondary messenger in signaling pathways for several plant hormones. Despite its clear role in regulating plant development, its role in fruit development is not known. In an earlier study, we described ashort root(shr) mutant of tomato, whose phenotype results from hyperaccumulation of NO. The molecular mapping localizedshrlocus in 2.5 Mb region of chromosome 9. Theshrmutant showed sluggish growth, with smaller leaves, flowers and was less fertile than wild type. Theshrmutant also showed reduced fruit size and slower ripening of the fruits post-mature green stage to the red ripe stage. Comparison of the metabolite profiles ofshrfruits with wild-type fruits during ripening revealed a significant shift in the patterns. Inshrfruits intermediates of the tricarboxylic acid (TCA) cycle were differentially regulated than WT indicating NO affected the regulation of TCA cycle. The accumulation of several amino acids, particularly tyrosine, was higher, whereas most fatty acids were downregulated inshrfruits. Among the plant hormones at one or more stages of ripening, ethylene, Indole-3-acetic acid and Indole-3-butyric acid increased inshr, whereas abscisic acid declined. Our analyses indicate that the retardation of fruit growth and ripening inshrmutant likely results from the influence of NO on central carbon metabolism and endogenous phytohormones levels.
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Reddaiah
authors.initials: R
authors.lastname: Bodanapu
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Suresh K
authors.initials: SK
authors.lastname: Gupta
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Pinjari O
authors.initials: PO
authors.lastname: Basha
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Kannabiran
authors.initials: K
authors.lastname: Sakthivel
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.lastname: Sadhana
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Yellamaraju
authors.initials: Y
authors.lastname: Sreelakshmi
authors.affiliation: Repository of Tomato Genomics Resources, Department of Plant Sciences, School of Life Sciences, University of Hyderabad Hyderabad, India.
authors.forename: Rameshwar
authors.initials: R
authors.lastname: Sharma
keywords: fruit ripening; metabolites; molecular mapping; nitric oxide; tomato