title: Characterization and Diagnostic Application of Trypanosoma cruzi Trypomastigote Excreted-Secreted Antigens Shed in Extracellular Vesicles Released from Infected Mammalian Cells.
abstract: Chagas disease, caused byTrypanosoma cruzi, although endemic in many parts of Central and South America, is emerging as a global health threat through the potential contamination of blood supplies. Consequently, in the absence of a gold standard assay for the diagnosis of Chagas disease, additional antigens or strategies are needed. A proteomic analysis of the trypomastigote excreted-secreted antigens (TESA) associated with exosomal vesicles shed byT. cruziidentified ∼80 parasite proteins, with the majority beingtrans-sialidases. Mass spectrometry analysis of immunoprecipitation products performed using Chagas immune sera showed a marked enrichment in a subset of TESA proteins. Of particular relevance for diagnostic applications were the retrotransposon hot spot (RHS) proteins, which are absent inLeishmaniaspp., parasites that often confound diagnosis of Chagas disease. Interestingly, serological screens using recombinant RHS showed a robust immunoreactivity with sera from patients with clinical stages of Chagas ranging from asymptomatic to advance cardiomyopathy and this immunoreactivity was comparable to that of crude TESA. More importantly, no cross-reactivity with RHS was detected with sera from patients with malaria, leishmaniasis, toxoplasmosis, or African sleeping sickness, making this protein an attractive reagent for diagnosis of Chagas disease.
authors.affiliation: Institute of Parasitology, McGill University, Montreal, Quebec, Canada.|Centre for Host Parasite-Interactions, McGill University, Montreal, Quebec, Canada.
authors.forename: Norma L
authors.initials: NL
authors.lastname: Bautista-López
authors.affiliation: Centre for Host Parasite-Interactions, McGill University, Montreal, Quebec, Canada.|National Reference Centre for Parasitology, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
authors.forename: Momar
authors.initials: M
authors.lastname: Ndao
authors.affiliation: Centre for Host Parasite-Interactions, McGill University, Montreal, Quebec, Canada.|National Reference Centre for Parasitology, Research Institute of the McGill University Health Centre, Montreal, Quebec, Canada.
authors.forename: Fabio Vasquez
authors.initials: FV
authors.lastname: Camargo
authors.affiliation: Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Nara
authors.affiliation: Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Annoura
authors.affiliation: University of Victoria Genome British Columbia Proteomics Centre, Victoria, Canada.
authors.forename: Darryl B
authors.initials: DB
authors.lastname: Hardie
authors.affiliation: University of Victoria Genome British Columbia Proteomics Centre, Victoria, Canada.
authors.forename: Christoph H
authors.initials: CH
authors.lastname: Borchers
authors.affiliation: Institute of Parasitology, McGill University, Montreal, Quebec, Canada Armando.jardim@mcgill.ca.|Centre for Host Parasite-Interactions, McGill University, Montreal, Quebec, Canada.
authors.forename: Armando
authors.initials: A
authors.lastname: Jardim
mesh_terms: D000913:Antibodies, Protozoan / Q000097:blood; D000953:Antigens, Protozoan / Q000032:analysis* / Q000276:immunology; D014355:Chagas Disease / Q000175:diagnosis*; D003429:Cross Reactions; D003430:Cross-Sectional Studies; D000067128:Extracellular Vesicles / Q000737:chemistry*; D006801:Humans; D013058:Mass Spectrometry; D020543:Proteome / Q000032:analysis*; D012680:Sensitivity and Specificity; D012698:Serologic Tests / Q000379:methods*; D014349:Trypanosoma cruzi / Q000737:chemistry*
chemical_list: D000913:Antibodies, Protozoan; D000953:Antigens, Protozoan; D020543:Proteome
keywords: TESA; Trypanosoma cruzi; parasite; proteomics; trypomastigote