title: Photodynamic effect of meso-(aryl)porphyrins and meso-(1-methyl-4-pyridinium)porphyrins on HaCaT keratinocytes.
abstract: Sixteen porphyrins, including neutral, anionic and cationic meso-(aryl)porphyrins and meso-(1-methyl-4-pyridinium)porphyrins were herein evaluated in terms of their photosensitizing properties against HaCaT keratinocytes. After an initial screening, the cationic porphyrins were studied in more details, by both determining their log POWand performing PDT assays in lower porphyrin concentrations. Porphyrins presenting two or more adjacent positively charged groups, directly linked to the macrocycle meso positions, appeared to be the most effective photosensitizers. The present study also included the dicationic 5,10-diphenyl-15,20-di(1-methylpyridinium-4-yl)porphyrin (14b), which has previously shown promising results on a psoriasis-like in vivo model. Overall results indicated that the beneficial effect related to porphyrins on psoriasis can be related to the decreasing of keratinocyte viability. Furthermore, some of the cationic porphyrins studied appeared as candidates to be utilized as photosensitizers for psoriasis treatment.
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil.
authors.forename: Amanda M
authors.initials: AM
authors.lastname: Slomp
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil.
authors.forename: Sandra M W
authors.initials: SMW
authors.lastname: Barreira
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil.
authors.forename: Luise Z B
authors.initials: LZB
authors.lastname: Carrenho
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil.
authors.forename: Camila C
authors.initials: CC
authors.lastname: Vandresen
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil.
authors.forename: Ingrid F
authors.initials: IF
authors.lastname: Zattoni
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil.
authors.forename: Stephanie M S
authors.initials: SMS
authors.lastname: Ló
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil.
authors.forename: Juliana C C
authors.initials: JCC
authors.lastname: Dallagnol
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Paraná, Brazil.
authors.forename: Diogo R B
authors.initials: DRB
authors.lastname: Ducatti
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Paraná, Brazil.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Orsato
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Paraná, Brazil.
authors.forename: M Eugênia R
authors.initials: MER
authors.lastname: Duarte
authors.affiliation: Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, PO Box 19046, Curitiba, Paraná, Brazil.
authors.forename: Miguel D
authors.initials: MD
authors.lastname: Noseda
authors.affiliation: Departamento de Farmacologia, Universidade Federal do Paraná, PO Box 19031, Curitiba, Paraná, Brazil.
authors.forename: Michel F
authors.initials: MF
authors.lastname: Otuki
authors.affiliation: Departamento de Farmácia, Universidade Federal do Paraná, Av. Lothario Meissner, 3400, Jardim Botânico, Curitiba, Paraná, Brazil. Electronic address: alan.goncalves@ufpr.br.
authors.forename: Alan G
authors.initials: AG
authors.lastname: Gonçalves
mesh_terms: D002460:Cell Line; D006801:Humans; D015603:Keratinocytes / Q000166:cytology / Q000187:drug effects*; D008027:Light; D017319:Photosensitizing Agents / Q000138:chemical synthesis / Q000494:pharmacology*; D011166:Porphyrins / Q000138:chemical synthesis / Q000494:pharmacology*; D011565:Psoriasis / Q000188:drug therapy; D011726:Pyridinium Compounds / Q000138:chemical synthesis / Q000494:pharmacology*
chemical_list: C000622944:5,10-diphenyl-15,20-di(1-methylpyridinium-4-yl)porphyrin; D017319:Photosensitizing Agents; D011166:Porphyrins; D011726:Pyridinium Compounds
keywords: HaCaT keratinocytes; Photodynamic therapy (PDT); Photosensitizer; Porphyrins; Psoriasis