title: Enzymatic synthesis of human blood group P1 pentasaccharide antigen.
abstract: The enzymatic synthesis of biologically important and structurally unique human P1PK blood group type P1 pentasaccharide antigen is described. This synthesis features a three-step sequential one-pot multienzyme (OPME) glycosylation for the stepwise enzymatic chain elongation of readily available lactoside acceptor with cheap and commercially available galactose and N-acetylglucosamine as donor precursors. This enzymatic synthesis provides an operationally simple approach to access P1 pentasaccharide and its structurally related Gb3 and P1 trisaccharide epitopes.
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.
authors.forename: Dawa
authors.initials: D
authors.lastname: Tsering
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.
authors.forename: Congcong
authors.initials: C
authors.lastname: Chen
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Ye
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Han
authors.affiliation: Department of Pharmacy, Qilu Hospital, Shandong University, Jinan, 250012, China. Electronic address: qiludrug@163.com.
authors.forename: Bai-Qian
authors.initials: BQ
authors.lastname: Jing
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China.
authors.forename: Xian-Wei
authors.initials: XW
authors.lastname: Liu
authors.affiliation: Department of Chemistry, University of California, One Shields Avenue, Davis, CA, 95616, USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China; Key Laboratory of Chemical Biology of Natural Products (Ministry of Education), Institute of Biochemical and Biotechnological Drugs, Shandong University, Jinan, 250012, China.
authors.forename: Fengshan
authors.initials: F
authors.lastname: Wang
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China; Shandong Academy of Pharmaceutical Science, Jinan, 250101, China.
authors.forename: Peixue
authors.initials: P
authors.lastname: Ling
authors.affiliation: National Glycoengineering Research Center, Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, and School of Pharmaceutical Sciences, Shandong University, Jinan, 250012, China; State Key Laboratory of Microbial Technology, Shandong University, Jinan, 250100, China. Electronic address: hzcao@sdu.edu.cn.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
mesh_terms: D000117:Acetylglucosamine / Q000737:chemistry*; D001789:Blood Group Antigens / Q000737:chemistry*; D005690:Galactose / Q000737:chemistry*; D006031:Glycosylation; D016695:Glycosyltransferases / Q000378:metabolism*; D006801:Humans; D009844:Oligosaccharides / Q000138:chemical synthesis*
chemical_list: D001789:Blood Group Antigens; D009844:Oligosaccharides; D016695:Glycosyltransferases; D000117:Acetylglucosamine; D005690:Galactose
keywords: Glycosylation; Glycosyltransferase; Human blood group; One-pot multienzyme; P1 antigen