title: Biochemical Purification of Binding Partners of Synaptic Scaffold Proteins.
abstract: The chemical synapse displays specialized intercellular adhesion between pre- and potsynaptic plasma membranes mediated by synaptic cell adhesion proteins. In this asymmetric cell adhesion, pre- and postsynapses have their own unique functions; the presynaptic terminal releases neurotransmitter, which diffuses through the synaptic cleft and is received by receptors accumulated at the postsynapse. Such distinct modes of actions of pre- and postsynapses in synaptic neurotransmission are the rate-limiting factors in signal processing in the brain, and thus protein-protein interactions within the pre- and postsynaptic scaffold are of particular importance for brain function by regulating the pre- and postsynaptic function. In the present paper, we outline a method to screen for binding partners of synaptic scaffold proteins biochemically.
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075, Göttingen, Germany. altas@em.mpg.de.
authors.forename: Bekir
authors.initials: B
authors.lastname: Altas
authors.affiliation: Proteomics Group, Max Planck Institute of Experimental Medicine, 37075, Göttingen, Germany.
authors.forename: Olaf
authors.initials: O
authors.lastname: Jahn
authors.affiliation: Department of Molecular Neurobiology, Max Planck Institute of Experimental Medicine, 37075, Göttingen, Germany. kawabe@em.mpg.de.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Kawabe
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism; D002352:Carrier Proteins / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism*; D002845:Chromatography / Q000379:methods; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D051379:Mice; D011485:Protein Binding; D011993:Recombinant Fusion Proteins / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013569:Synapses / Q000378:metabolism*
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D002352:Carrier Proteins; D011993:Recombinant Fusion Proteins
keywords: Affinity purification; Binding partners; Glutathione-S-Transferase (GST) -fused proteins; Mass spectrometry; Mouse brain extract; Synaptic scaffold proteins