title: Long Noncoding RNA DANCR Is a Positive Regulator of Proliferation and Chondrogenic Differentiation in Human Synovium-Derived Stem Cells.
abstract: Cartilage tissues have limited capacity for repair after damage and then cause osteoarthritis, so finding alternative treatment is ongoing. Mesenchymal stem cells (MSCs) have become a promising therapy for cartilage damage and diseases due to the advantages of easy separation, high proliferative potentiality, and genetic stability. Synovium-derived MSCs (SMSCs) have been recognized as an ideal source for cartilage repair. In our previous study, we found that Sox4 promoted proliferation and chondrogenesis of SMSCs through upregulation of long noncoding RNA (lncRNA) DANCR. However, the exact molecular mechanism by which DANCR promotes proliferation and chondrogenesis of SMSCs remains unknown. In the present study, we investigated the effect of lncRNA DANCR on the proliferation and chondrogenesis of SMSCs. We found that overexpression of DANCR could promote proliferation and chondrogenesis of SMSCs, while knockdown of DANCR had the opposite effect. Moreover, our data demonstrated that DANCR directly interacted with myc, Smad3, and STAT3 mRNA to regulate their stability. Finally, we found that the promotion of SMSC proliferation induced by DANCR depended on myc. Also, DANCR activated chondrogenesis of SMSCs via upregulation of Smad3 and STAT3 expression. Our growing knowledge of the role of DANCR is pointing toward its potential use as a novel therapeutic approach for cartilage damage and diseases.
authors.affiliation: 1 Department of Orthopedics, Jinling Hospital, Nanjing University School of Medicine , Nanjing, China .
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: 1 Department of Orthopedics, Jinling Hospital, Nanjing University School of Medicine , Nanjing, China .
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: 1 Department of Orthopedics, Jinling Hospital, Nanjing University School of Medicine , Nanjing, China .
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: 2 Department of Orthopedics, Nanjing Pukou Central Hospital , Nanjing, China .
authors.forename: Guihua
authors.initials: G
authors.lastname: Wang
authors.affiliation: 3 Department of Orthopedics, Nanjing General Hospital, The Second Military Medical University Clinical Medical School of Nanjing , Nanjing, China .
authors.forename: Ben
authors.initials: B
authors.lastname: Shi
authors.affiliation: 4 Department of Orthopedics, People's Hospital of Liyang , Liyang, China .
authors.forename: Xin
authors.initials: X
authors.lastname: Tao
authors.affiliation: 1 Department of Orthopedics, Jinling Hospital, Nanjing University School of Medicine , Nanjing, China .
authors.forename: Liwu
authors.initials: L
authors.lastname: Zhou
authors.affiliation: 1 Department of Orthopedics, Jinling Hospital, Nanjing University School of Medicine , Nanjing, China .
authors.forename: Jianning
authors.initials: J
authors.lastname: Zhao
mesh_terms: D001483:Base Sequence; D002454:Cell Differentiation / Q000235:genetics*; D049109:Cell Proliferation / Q000235:genetics; D020219:Chondrogenesis / Q000235:genetics*; D055785:Gene Knockdown Techniques; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D062085:RNA, Long Noncoding / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000235:genetics; D051900:Smad3 Protein / Q000235:genetics; D013583:Synovial Membrane / Q000166:cytology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: D016271:Proto-Oncogene Proteins c-myc; D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D050796:STAT3 Transcription Factor; D051900:Smad3 Protein
keywords: DANCR; STAT3; Smad3; chondrogenic differentiation; myc; synovium-derived stem cell