title: Two ovine mitochondrial DNAs harboring a fifth 75/76 bp repeat motif without altered gene expression in Northern Spain.
abstract: The Basque Country is home to the Latxa sheep breed, which is divided in several varieties such as Latxa Black Face (LBKF) and Latxa Blonde Face (LBLF). Mitochondrial DNA control region analysis of 174 male sheep (97 LBKF and 77 LBLF) was performed with the objective of characterizing the maternal lineages of these two varieties that are the basis to produce the cheese with Idiazabal quality label. The percentage of unique haplotypes was 77.32% in LBKF and 67.53% in LBLF. Most of the individuals were classified into B haplogroup (98.85%), while A haplogroup was much less frequent. Two Latxa individuals (one LBKF and one LBLF), both belonging to B haplogroup, displayed an additional 75/76 bp tandem repeat motif. Only 33 other sequences with this repeat motif were found among 11 061 sheep sequences included in the GenBank database. Gene expression was analyzed in peripheral blood leukocytes since the additional 75/76 bp repeat motif falls within ETAS1, a domain with a possible function in regulation of replication and transcription. The mRNA expression from four mitochondrial genes (COI, cyt b, ND1, and ND2) was analyzed in the two individuals of this study with a fifth repeat motif and in four without it. Although lower transcription was observed when the additional 75/76 bp repeat motif was present, no statistically significant differences were observed. Therefore, the variation in the number of the 75/76 repeat motif does not seem to modify the gene expression rate in mitochondrial genes.
authors.affiliation: BIOMICs Research Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Lopez-Oceja
authors.affiliation: BIOMICs Research Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
authors.forename: D
authors.initials: D
authors.lastname: Gamarra
authors.affiliation: BIOMICs Research Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
authors.forename: S
authors.initials: S
authors.lastname: Cardoso
authors.affiliation: BIOMICs Research Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
authors.forename: L
authors.initials: L
authors.lastname: Palencia-Madrid
authors.affiliation: Animal Health Department, Instituto Vasco de Investigación y Desarrollo Agrario (NEIKER), Derio, Bizkaia, Spain.
authors.forename: R A
authors.initials: RA
authors.lastname: Juste
authors.affiliation: BIOMICs Research Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain.
authors.forename: M M
authors.initials: MM
authors.lastname: De Pancorbo
mesh_terms: D000818:Animals; D001483:Base Sequence; D004272:DNA, Mitochondrial / Q000097:blood* / Q000235:genetics; D005786:Gene Expression Regulation; D050259:Genes, Mitochondrial; D014644:Genetic Variation; D006239:Haplotypes; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods; D012333:RNA, Messenger / Q000097:blood; D012756:Sheep / Q000235:genetics*; D013030:Spain; D020080:Tandem Repeat Sequences
chemical_list: D004272:DNA, Mitochondrial; D012333:RNA, Messenger
keywords: Control region; Gene expression; Mitochondrial DNA; Ovis aries