title: Investigation of malignant transformation of sporadic adenomas of colon by fluorimetry.
abstract: In this work, biochemical changes in sporadic adenoma during progression to colon cancer have been detected by spectral techniques. It is shown that spectra of all types of adenomas as well as adenocarcinoma have two maxima at 265 ÷ 270 nm and at 330 ÷ 340 nm. The first maximum is primarily defined by tryptophan containing peptides. The second maximum of the spectra is in the main defined by collagen presence in stroma of tumors and normal mucosa. Progression of precancer lesions to advanced cancer leads to increase of NADH concentration impacting on the second maximum of spectra. At the time, the spectra of both AC differ from each other by widths of the second maximum. For well-differentiated AC, the second maximum is wider. It gives reason to suggest that two fluorophores impact on its intensity. These fluorophores can be NADH and vitamin B6. Correlation between microscopical and spectral results can allow to use fluorimetry for detail diagnostics and investigation of colon cancer development.
authors.affiliation: Smolensk State Medical University, Krupskoy 28 st., Smolensk, Russia.|Smolensk Regional Institute of Pathology, Gagarina st, 27, Smolensk, Russia.
authors.forename: Yu S
authors.initials: YS
authors.lastname: Korneva
authors.affiliation: Smolensk State Medical University, Krupskoy 28 st., Smolensk, Russia.|Smolensk Regional Institute of Pathology, Gagarina st, 27, Smolensk, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Dorosevich
authors.affiliation: Orenburg State University, Pobedy 13 st., Orenburg, Russia. valemar@mail.ru.
authors.forename: V S
authors.identifier: http://orcid.org/0000-0002-9662-4146
authors.initials: VS
authors.lastname: Maryakhina
mesh_terms: D000230:Adenocarcinoma / Q000473:pathology; D000236:Adenoma / Q000473:pathology*; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D003106:Colon / Q000473:pathology; D003110:Colonic Neoplasms / Q000473:pathology*; D005260:Female; D005470:Fluorometry / Q000379:methods*; D006801:Humans; D008297:Male; D008875:Middle Aged; D013050:Spectrometry, Fluorescence
keywords: Adenocarcinoma; Adenoma; Cancerogenesis; Colon cancer; Fluorimetry