title: Hesa-A Effects on Cell Cycle Signaling in Esophageal Carcinoma Cell Line.
abstract: BACKGROUND Hesa-A is a natural compound with anticancer properties. The exact mechanism of its action in esophageal cancer is not clear, yet. The aim of this study was to evaluate the cell toxicity effect of Hesa-A on the esophageal carcinoma cell lines, KYSE-30, and cell cycle genes expression. METHODS In this study, we tested cell toxicity with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) assay and flow cytometry to evaluatet he cell cycle arrest. Real time polymerase chain reaction was used to assess the expression of P53, P16, P21, cyclin D1, and cyclin B1 genes. RESULTS Our results showed that Hesa-A is effective in the expression of cell cycling check point proteins. Hesa-A induced an arrest in G2 phase of esophageal cell cycle. The levels of P53 (>13 times), P21 (>21 times), P16, cyclin B1, and cyclin D1 genes were increased 48 hours after Hesa-A treatment. CONCLUSION P21 and P16 expression were the potential mechanisms for G2 arrest of KYSE-30 esophageal cancer cell line by Hesa-A.
authors.affiliation: Faculty of Advanced Medical Science Technology, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Ahmadian
authors.affiliation: Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Roghiyeh
authors.initials: R
authors.lastname: Pashaei-Asl
authors.affiliation: Faculty of Advanced Biomedical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Nasser
authors.initials: N
authors.lastname: Samadi
authors.affiliation: Department of Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Rahmati-Yamchi
authors.affiliation: Research Center for Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Mohammad-Reza
authors.initials: MR
authors.lastname: Rashidi
authors.affiliation: Faculty of Advanced Medical Science Technology, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Masomeh
authors.initials: M
authors.lastname: Ahmadian
authors.affiliation: Women's Reproductive Health Research Center, Alzahra Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Moosa
authors.initials: M
authors.lastname: Esmaeili
authors.affiliation: Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Salamat
authors.affiliation: Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Sima
authors.initials: S
authors.lastname: Besharat
authors.affiliation: Golestan Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Joshaghani
keywords:  Cyclin B1 gene.; Cyclin D1 gene; Esophageal cancer; Flow cytometry; Hesa-A; P16gene; P21 gene; P53 gene; Real Time PCR