title: Diagnosis of common hemoglobinopathies among South East Asian population using capillary isoelectric focusing system.
abstract: BACKGROUND
  We have evaluated an automated capillary isoelectric focusing (cIEF)-based Hb analyzer in diagnosis of hemoglobinopathies commonly found among South East Asian population.
  
  
  METHODS
  Study was performed on a cohort of 665 adult Thai subjects and 13 fetal blood specimens obtained at routine thalassemia diagnostic laboratory. Hb analysis was performed using the cIEF system. Thalassemia genotypes were defined by DNA analysis.
  
  
  RESULTS
  The system revealed satisfactorily within-run and between-run precision for quantitation of Hb A2and Hb E (CV: 0.02-0.09%). The reference ranges of Hb A2and Hb E were 2.6-4.0% and 25.7-33.1%, respectively. The system identified the cases of β-thalassemia and Hb E disorders correctly. Several thalassemia genotypes and Hb variants were identifiable. However, Hb Constant Spring was separated closely to Hb A2and Hbs Bart's and H were relatively difficult to be reported due to interfering peaks separating at the same regions. Prenatal diagnosis by fetal blood analysis was found to be accurate for Hb Bart's hydrops fetalis and Hb E-β0-thalassemia disease.
  
  
  CONCLUSIONS
  The cIEF system could accurately diagnose β-thalassemia and Hb E carriers and demonstrate many Hb variants found in the region. The system cannot report Hb A2in the presence of Hb E whereas Hbs Lepore and F are comigrated. Diagnosis of α-thalassemia disease based on Hb H and Hb Bart's might be difficult.
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: H
authors.initials: H
authors.lastname: Srivorakun
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: G
authors.initials: G
authors.lastname: Fucharoen
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: K
authors.initials: K
authors.lastname: Sanchaisuriya
authors.affiliation: Centre for Research and Development of Medical Diagnostic Laboratories, Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: S
authors.initials: S
authors.lastname: Fucharoen
mesh_terms: D000328:Adult; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D005260:Female; D006455:Hemoglobins, Abnormal / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007525:Isoelectric Focusing / Q000295:instrumentation / Q000379:methods; D008297:Male; D011237:Predictive Value of Tests; D011296:Prenatal Diagnosis / Q000295:instrumentation / Q000379:methods; D013785:Thailand / Q000453:epidemiology; D013789:Thalassemia / Q000097:blood* / Q000453:epidemiology / Q000235:genetics
chemical_list: D006455:Hemoglobins, Abnormal
keywords: Hemoglobinopathies; capillary isoelectric focusing; hemoglobin analysis; thalassemia