title: An optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species.
abstract: The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and quality. Several genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp species. However, all current protocols require invasive handling methods with males for DNA isolation. Using Aristeus antennatus as a model we tested a reliable non-invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female thelycum. The present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male genotyping. This new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding programs. More importantly, it can be applied to identify the mating pairs and male structure in wild populations of species as A. antennatus, where males are often difficult to capture. Our method could be also valuable for biological studies on other spermatophore-using species, such as myriapods, arachnids and insects.
authors.affiliation: Laboratory of Genetic Ichthyology, University of Girona, Girona, Spain.
authors.forename: Laia
authors.initials: L
authors.lastname: Planella
authors.affiliation: Laboratory of Genetic Ichthyology, University of Girona, Girona, Spain.
authors.forename: Sandra
authors.initials: S
authors.lastname: Heras
authors.affiliation: Laboratory of Genetic Ichthyology, University of Girona, Girona, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Vera
authors.affiliation: Laboratory of Genetic Ichthyology, University of Girona, Girona, Spain.
authors.forename: José-Luis
authors.initials: JL
authors.lastname: García-Marín
authors.affiliation: Laboratory of Genetic Ichthyology, University of Girona, Girona, Spain.
authors.forename: María Inés
authors.initials: MI
authors.lastname: Roldán
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis; D005260:Female; D005838:Genotype; D008297:Male; D033561:Penaeidae / Q000235:genetics*; D016133:Polymerase Chain Reaction; D012098:Reproduction; D013093:Spermatogonia
chemical_list: D004247:DNA
keywords: differential spermatophore DNA extraction; high quality DNA; male genotyping; non-invasive DNA extraction; open thelycum shrimp