title: Selection and Validation of Reference Genes for Gene Expression Analysis in Vigna angularis Using Quantitative Real-Time RT-PCR.
abstract: Adzuki bean (Vigna angularis) is one of the most important legume crops in Asian countries like China, Japan and Korea due to its nutritious protein and starch contents. In spite of its economic importance, gene expression analysis system for gene function verification of adzuki bean is still absent. Therefore, reference genes for gene expression analysis based on the quantitative real time PCR (qRT-PCR) were screened in current study. A total of nine general housekeeping genes, including ACT, Fbox, ZMPP, GAPDH, EF, PP2A, UBC, UBN and PTB were evaluated for their expression stability by qRT-PCR in four adzuki bean cultivars, three different tissues, four abiotic stress and one biotic stress. The best group of candidates as reference genes were as follows: PTB and ACT for different cultivars; EF and UBN for different tissues; ACT and ZMPP for biotic stress and waterlogging stress; Fbox and UBC for salinity-alkalinity stress; Fbox and PTB for drought stress. Our results will provide a more accurate and reliable normalization of qRT-PCR data in adzuki bean.
authors.affiliation: Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chi
authors.affiliation: Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Yin
authors.affiliation: Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Xiwang
authors.initials: X
authors.lastname: Ke
authors.affiliation: Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Dong
authors.initials: D
authors.lastname: Han
authors.affiliation: Heilongjiang Bayi Agricultural University, Daqing, Heilongjiang, People's Republic of China.
authors.forename: Yuhu
authors.identifier: http://orcid.org/0000-0001-5718-9738
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D018556:Crops, Agricultural / Q000235:genetics; D055864:Droughts; D020869:Gene Expression Profiling / Q000592:standards*; D018506:Gene Expression Regulation, Plant; D020043:Genes, Essential; D010940:Plant Proteins / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000592:standards; D012015:Reference Standards; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000592:standards; D054712:Salinity; D013312:Stress, Physiological; D000070658:Vigna / Q000235:genetics* / Q000254:growth & development
chemical_list: D010940:Plant Proteins