title: Knockdown of HOXA5 inhibits the tumorigenesis in esophageal squamous cell cancer.
abstract: Homeobox A5 (HOXA5) is a member of the homeobox (HOX) family and was upregulated in many types of tumors. However, its expression and role in esophageal squamous cell carcinoma (ESCC) remain unclear. In this study, the aim of this study was to investigate the expression and function of HOXA5 in ESCC. Our results showed that HOXA5 was highly expressed in ESCC cell lines. The in vitro experiments demonstrated that knockdown of HOXA5 significantly inhibited the proliferation, migration and invasion of ESCC cells. Furthermore, the in vivo experiments showed that knockdown of HOXA5 significantly inhibited the tumor growth of ESCC in mice xenograft model. Finally, sh-HOXA5 inhibited the expression of β-catenin, cyclin D1 and c-Myc in ESCC cells. Taken together, these data revealed that knockdown of HOXA5 suppressed the proliferation and metastasis partly by interfering with Wnt/β-catenin signaling pathway in ESCC cells. Therefore, these findings suggest that HOXA5 may be a potential therapeutic target for the treatment of ESCC.
authors.affiliation: Department of Digestive Medicine, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Digestive Medicine, Huaihe Hospital of Henan University, Kaifeng 475000, China.
authors.forename: Jiang-Hai
authors.initials: JH
authors.lastname: Zhao
authors.affiliation: Department of Digestive Medicine, Huaihe Hospital of Henan University, Kaifeng 475000, China. Electronic address: suo_zhimin@163.com.
authors.forename: Zhi-Min
authors.initials: ZM
authors.lastname: Suo
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000235:genetics* / Q000473:pathology; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D004938:Esophageal Neoplasms / Q000235:genetics* / Q000473:pathology; D000077277:Esophageal Squamous Cell Carcinoma; D055785:Gene Knockdown Techniques / Q000379:methods*; D018398:Homeodomain Proteins / Q000037:antagonists & inhibitors / Q000235:genetics*; D006801:Humans; D051379:Mice; D008819:Mice, Nude; D023041:Xenograft Model Antitumor Assays / Q000379:methods
chemical_list: C489213:HOXA5 protein, human; D018398:Homeodomain Proteins
keywords: Esophageal squamous cell carcinoma (ESCC); Homeobox A5 (HOXA5); Migration; Proliferation