title: Probing the orientation of inhibitor and epoxy-eicosatrienoic acid binding in the active site of soluble epoxide hydrolase.
abstract: Soluble epoxide hydrolase (sEH) is an important therapeutic target of many diseases, such as chronic obstructive pulmonary disease (COPD) and diabetic neuropathic pain. It acts by hydrolyzing and thus regulating specific bioactive long chain polyunsaturated fatty acid epoxides (lcPUFA), like epoxyeicosatrienoic acids (EETs). To better predict which epoxides could be hydrolyzed by sEH, one needs to dissect the important factors and structural requirements that govern the binding of the substrates to sEH. This knowledge allows further exploration of the physiological role played by sEH. Unfortunately, a crystal structure of sEH with a substrate bound has not yet been reported. In this report, new photoaffinity mimics of a sEH inhibitor and EET regioisomers were prepared and used in combination with peptide sequencing and computational modeling, to identify the binding orientation of different regioisomers and enantiomers of EETs into the catalytic cavity of sEH. Results indicate that the stereochemistry of the epoxide plays a crucial role in dictating the binding orientation of the substrate.
authors.affiliation: Department of Entomology and Nematology, UCD Comprehensive Cancer Center, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Kin Sing Stephen
authors.initials: KS
authors.lastname: Lee
authors.affiliation: Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, 9500 Gilman Drive, MC 0736, La Jolla, CA 92093, USA.
authors.forename: Niel M
authors.initials: NM
authors.lastname: Henriksen
authors.affiliation: Department of Entomology and Nematology, UCD Comprehensive Cancer Center, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Connie J
authors.initials: CJ
authors.lastname: Ng
authors.affiliation: Department of Entomology and Nematology, UCD Comprehensive Cancer Center, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Yang
authors.affiliation: Campus Mass Spectrometry Facilities, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Weitao
authors.initials: W
authors.lastname: Jia
authors.affiliation: Department of Entomology and Nematology, UCD Comprehensive Cancer Center, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Christophe
authors.initials: C
authors.lastname: Morisseau
authors.affiliation: Campus Mass Spectrometry Facilities, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA.
authors.forename: Armann
authors.initials: A
authors.lastname: Andaya
authors.affiliation: Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California at San Diego, 9500 Gilman Drive, MC 0736, La Jolla, CA 92093, USA.
authors.forename: Michael K
authors.initials: MK
authors.lastname: Gilson
authors.affiliation: Department of Entomology and Nematology, UCD Comprehensive Cancer Center, University of California at Davis, One Shields Avenue, Davis, CA 95616, USA. Electronic address: bdhammock@ucdavis.edu.
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Hammock
mesh_terms: D001095:Arachidonic Acids / Q000737:chemistry*; D002264:Carboxylic Acids / Q000737:chemistry; D002384:Catalysis; D020134:Catalytic Domain; D003198:Computer Simulation; D003460:Crystallization; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry; D004851:Epoxide Hydrolases / Q000737:chemistry*; D004852:Epoxy Compounds / Q000737:chemistry; D004926:Escherichia coli / Q000378:metabolism; D006801:Humans; D006868:Hydrolysis; D020128:Inhibitory Concentration 50; D008027:Light; D013058:Mass Spectrometry; D056004:Molecular Dynamics Simulation; D010455:Peptides / Q000737:chemistry; D011994:Recombinant Proteins / Q000737:chemistry; D012997:Solvents / Q000737:chemistry; D013237:Stereoisomerism; D013379:Substrate Specificity; D014357:Trypsin / Q000737:chemistry
chemical_list: D001095:Arachidonic Acids; D002264:Carboxylic Acids; D004852:Epoxy Compounds; D010455:Peptides; D011994:Recombinant Proteins; D012997:Solvents; D003577:Cytochrome P-450 Enzyme System; D004851:Epoxide Hydrolases; D014357:Trypsin
keywords: Computational simulation; Epoxyeicosatrienoic acid; Peptide sequencing; Photoaffinity tag; Photolabel; Soluble epoxide hydrolase