title: Modified technique for preparation of venous circulation resin casts in the cirrhotic liver.
abstract: This study describes two major adaptations for the preparation of resin casts in human cirrhotic liver, harvested at the time of transplantation. The first is the way of fixing the catheter in the ostia of the hepatic and portal veins through a cerclage, so as to prevent displacement of the catheter and / or leakage of the resin during its injection. The second is the extension of corrosion time in the NaOH solution, averaging 6.8 days, with daily replacement the solution until complete removal of parenchymal tissue. We applied the method in 14 cirrhotic livers, with good filling and coloring of the portal and hepatic vein territories, using different colors. This allows an anatomical study of these vessels, able to complement the knowledge of the histopathology in research work, and the planning of therapeutic procedures, such as the Trans-Jugular Intrahepatic Port-Systemic Shunt (TIPS). RESUMO Este estudo descreve duas importantes adaptações para o preparo de moldes de resina em fígado humano cirrótico, captado no momento do transplante: a primeira, é a maneira de fixação dos cateteres nos "óstios" das veias hepáticas e porta, através de uma "cerclagem" dos mesmos, de modo a evitar o deslocamento do cateter e/ou extravasamento da resina durante sua injeção, e a segunda, é o prolongamento do tempo de corrosão na solução de NaOH, atingindo a média de 6,8 dias, com a substituição diária da solução, até a remoção completa do tecido parenquimatoso. O método foi empregado em 14 fígados cirróticos com bom preenchimento e coloração dos territórios das veias porta e hepáticas, utilizando cores distintas. Isto permite um estudo anatômico desses vasos, capaz de complementar os conhecimentos da histopatologia em trabalhos de pesquisa, e planejar procedimentos terapêuticos como a derivação porto-sistêmica intra-hepática transjugular (TIPS - Transjugular Intrahepatic Postosystemic Shunt).
authors.affiliation: Faculty of Medical Sciences (FCM), Pernambuco University (UPE), Recife, PE, Brazil.
authors.forename: José Olímpio Maia DE
authors.initials: JO
authors.lastname: Vasconcelos
authors.affiliation: Oswaldo University Cruz Hospital, Pernambuco University (UPE), Recife, PE, Brazil.
authors.forename: Laécio Leitão
authors.initials: LL
authors.lastname: Batista
authors.affiliation: School of Medical Sciences, Alagoas State University of Health Sciences (UNCISAL), Maceio, AL, Brazil.
authors.forename: Guilherme Benjamin Brandão
authors.initials: GB
authors.lastname: Pitta
authors.affiliation: School of Medical Sciences, Alagoas State University of Health Sciences (UNCISAL), Maceio, AL, Brazil.
authors.forename: Cláudio Moura
authors.initials: CM
authors.lastname: Lacerda
mesh_terms: D000180:Acrylic Resins; D000328:Adult; D000368:Aged; D005260:Female; D006503:Hepatic Veins / Q000033:anatomy & histology*; D006801:Humans; D008099:Liver / Q000098:blood supply*; D008102:Liver Circulation; D008103:Liver Cirrhosis / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D008953:Models, Anatomic; D011169:Portal Vein / Q000033:anatomy & histology*; D055815:Young Adult
chemical_list: D000180:Acrylic Resins