title: An injectable collagen/poly(γ-glutamic acid) hydrogel as a scaffold of stem cells and α-lipoic acid for enhanced protection against renal dysfunction.
abstract: Mesenchymal stem cells (MSCs) can ameliorate renal injury and accelerate repair of acute kidney injury. Herein, we developed a collagen/poly(γ-glutamic acid) (γ-PGA) hydrogel as an injectable scaffold for the delivery of mouse MSCs (mMSCs) and anti-oxidant drugs into injured sites. By the introduction of γ-PGA into conventional collagen, the viscosity of collagen was reduced at ambient temperature for easy handling, while the elastic and viscous moduli of collagen were increased and a new porous structure was generated near body temperature. When in situ gel-forming collagen/γ-PGA hydrogels loaded with mMSCs and α-lipoic acid (LA) were administered to a mouse model of renal dysfunction, they significantly attenuated the level of blood urea nitrogen and creatinine, which resulted from the increased retention of therapeutic mMSCs and the controlled release of anti-oxidant drugs at the injured site. These findings suggested that this novel type of hydrogel could be applied as an injectable scaffold for use in regenerative medicine.
authors.affiliation: SKKU Advanced Institute of Nanotechnology (SAINT), School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. yongtaik@skku.edu.
authors.forename: Sun-Hee
authors.initials: SH
authors.lastname: Cho
authors.affiliation: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea. chullee@kribb.re.kr.
authors.forename: Jung-Ran
authors.initials: JR
authors.lastname: Noh
authors.affiliation: SKKU Advanced Institute of Nanotechnology (SAINT), School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. yongtaik@skku.edu.
authors.forename: Mi Young
authors.initials: MY
authors.lastname: Cho
authors.affiliation: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea. chullee@kribb.re.kr.
authors.forename: Min-Jeong
authors.initials: MJ
authors.lastname: Go
authors.affiliation: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea. chullee@kribb.re.kr.
authors.forename: Yong-Hoon
authors.initials: YH
authors.lastname: Kim
authors.affiliation: SKKU Advanced Institute of Nanotechnology (SAINT), School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. yongtaik@skku.edu.
authors.forename: Eun Sung
authors.initials: ES
authors.lastname: Kang
authors.affiliation: SKKU Advanced Institute of Nanotechnology (SAINT), School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. yongtaik@skku.edu.
authors.forename: Yong Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Laboratory Animal Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea. chullee@kribb.re.kr.
authors.forename: Chul-Ho
authors.initials: CH
authors.lastname: Lee
authors.affiliation: SKKU Advanced Institute of Nanotechnology (SAINT), School of Chemical Engineering, Sungkyunkwan University, Suwon 16419, Republic of Korea. yongtaik@skku.edu.
authors.forename: Yong Taik
authors.identifier: http://orcid.org/0000-0001-5558-5526
authors.initials: YT
authors.lastname: Lim
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin; D003094:Collagen / Q000008:administration & dosage / Q000737:chemistry*; D004195:Disease Models, Animal; D020100:Hydrogels / Q000008:administration & dosage / Q000737:chemistry*; D007674:Kidney Diseases / Q000188:drug therapy / Q000473:pathology / Q000517:prevention & control / Q000628:therapy*; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D051379:Mice; D011099:Polyglutamic Acid / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D008063:Thioctic Acid / Q000494:pharmacology* / Q000627:therapeutic use; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D020100:Hydrogels; C511775:poly(gamma-glutamic acid); D011099:Polyglutamic Acid; D008063:Thioctic Acid; D003094:Collagen; D002945:Cisplatin