title: BmNHR96 participate BV entry of BmN-SWU1 cells via affecting the cellular cholesterol level.
abstract: B.mori nucleopolyhedrovirus (BmNPV), which produces BV and ODV two virion phenotypes in its life cycle, caused the amount of economic loss in sericulture. But the mechanism of its infection was still unclear. In this study we characterized B.mori nuclear hormone receptor 96 (BmNHR96) as a NHR96 family member, which was localized in the nucleus. We also found BmNHR96 over-expression could enhance the entry of BV as well as cellular cholesterol level. Furthermore, we validated that BmNHR96 increased membrane fusion mediated by GP64, which could probably promote BV-infection. In summary, our study suggested that BmNHR96 plays an important role in BV infection and this function probably actualized by affecting cellular cholesterol level, and our results provided insights to the mechanisms of BV-infection of B.mori.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Tai-Hang
authors.initials: TH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Guo-Yu
authors.initials: GY
authors.lastname: Du
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China. Electronic address: pmh0476@hotmail.com.
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing, China. Electronic address: lucheng@swu.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
mesh_terms: D020816:Amino Acid Motifs; D000818:Animals; D012831:Bombyx / Q000378:metabolism / Q000821:virology*; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism; D002467:Cell Nucleus / Q000378:metabolism; D002784:Cholesterol / Q000737:chemistry; D003001:Cloning, Molecular; D003593:Cytoplasm / Q000378:metabolism; D008561:Membrane Fusion; D021962:Membrane Microdomains / Q000737:chemistry; D017924:Nucleopolyhedroviruses / Q000235:genetics* / Q000502:physiology; D010641:Phenotype; D018160:Receptors, Cytoplasmic and Nuclear / Q000378:metabolism*; D014764:Viral Proteins / Q000235:genetics*; D014779:Virus Replication
chemical_list: D018160:Receptors, Cytoplasmic and Nuclear; D014764:Viral Proteins; D002784:Cholesterol
keywords: BV infection; BmNHR96; Bombyx mori; Cholesterol