title: Osteoarthritis model induced by intra-articular monosodium iodoacetate in rats knee.
abstract: To evaluate the usefulness of a knee osteoarthritis model through functional, radiological and microscopic changes of the synovial membrane.
  Forty eight rats were divided randomly into two groups. The first received 0.9% saline in the joint and corresponded to the control group. The second was submitted to experimental osteoarthritis of the right knee induced by monosodium iodoacetate and corresponded to the osteoarthritis group. All animals were subjected to comparative tests of forced ambulation and joint movements, inability to articulate and tactile allodynia on day 1 post-experiment by forced ambulation (Roto-rod test), joint assessment of disability (weight bearing test) and assessment of tactile allodynia (Von Frey test). After inflammatory induction they were divided into four sub-groups corresponding to the scheduled death in 7, 14, 21 and 28 days when they were submitted to radiographic examination of the knee, arthrotomy and collection of the synovial membrane.
  The osteoarthritis group showed significant differences compared to control group on days 7 and 14 in Roto-rod, in weight bearing and Von Frey tests in all days, and in radiological evaluation. Microscopic examination of the synovial membrane showed abnormalities of inflammatory character at all stages.
  The osteoarthritis induced by intra-articular monosodium iodoacetate in rats knee is a good model to be used in related research, because it provides mensurable changes on joint movements, tactile allodynia, progressive radiological degeneration and microscopic inflammation of the synovial membrane, that represent markers for osteoarthritis evaluation.
authors.affiliation: Fellow Master degree, Postgraduate Program in Principles of Surgery, Faculdade Evangélica do Paraná (FEPAR), Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Conception, design, intellectual and scientific content of the study.
authors.forename: Sebastião Vieira de
authors.initials: SV
authors.lastname: Morais
authors.affiliation: Associate Professor, Postgraduate Program in Principles of Surgery, FEPAR, Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Scientific and intellectual content of the study, interpretation of data, critical revision.
authors.forename: Nicolau Gregori
authors.initials: NG
authors.lastname: Czeczko
authors.affiliation: Full Professor, Postgraduate Program in Principles of Surgery FEPAR, Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Scientific and intellectual content of the study, interpretation of data, critical revision.
authors.forename: Osvaldo
authors.initials: O
authors.lastname: Malafaia
authors.affiliation: Associate Professor, Postgraduate Program in Principles of Surgery, FEPAR, Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Scientific and intellectual content of the study, interpretation of data, critical revision.
authors.forename: Jurandir Marcondes
authors.initials: JM
authors.lastname: Ribas
authors.affiliation: Associate Professor, Universidade Federal do Maranhão (UFMA), Sao Luiz-MA, Brazil. Conception, design, intellectual and scientific content of the study.
authors.forename: João Batista Santos
authors.initials: JB
authors.lastname: Garcia
authors.affiliation: Fellow Master degree, Postgraduate Program in Principles of Surgery, Faculdade Evangélica do Paraná (FEPAR), Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Conception, design, intellectual and scientific content of the study.
authors.forename: Marcelo Tizzot
authors.initials: MT
authors.lastname: Miguel
authors.affiliation: Fellow Master degree, Postgraduate Program in Principles of Surgery, Faculdade Evangélica do Paraná (FEPAR), Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Conception, design, intellectual and scientific content of the study.
authors.forename: Cassio
authors.initials: C
authors.lastname: Zini
authors.affiliation: Graduate student, FEPAR, Universidade Evangélica, Hospital de Curitiba, Medical Research Institute, Curitiba-PR, Brazil. Acquisition of data.
authors.forename: Angeline Garcez
authors.initials: AG
authors.lastname: Massignan
mesh_terms: D000818:Animals; D002358:Cartilage, Articular / Q000473:pathology*; D004195:Disease Models, Animal; D006930:Hyperalgesia / Q000139:chemically induced / Q000473:pathology / Q000503:physiopathology; D007270:Injections, Intra-Articular; D019807:Iodoacetic Acid / Q000008:administration & dosage / Q000009:adverse effects*; D007719:Knee Joint / Q000502:physiology / Q000503:physiopathology*; D008297:Male; D009068:Movement; D020370:Osteoarthritis, Knee / Q000139:chemically induced* / Q000473:pathology / Q000503:physiopathology; D051381:Rats; D017208:Rats, Wistar; D013583:Synovial Membrane / Q000000981:diagnostic imaging / Q000473:pathology*
chemical_list: D019807:Iodoacetic Acid