title: Influence of Surface Properties on Adhesion Forces and Attachment ofStreptococcus mutansto ZirconiaIn Vitro.
abstract: Zirconia is becoming a prevalent material in dentistry. However, any foreign bodies inserted may provide new niches for the bacteria in oral cavity. The object of this study was to explore the effect of surface properties including surface roughness and hydrophobicity on the adhesion and biofilm formation ofStreptococcus mutans(S. mutans) to zirconia. Atomic force microscopy was employed to determine the zirconia surface morphology and the adhesion forces between theS. mutansand zirconia. The results showed that the surface roughness was nanoscale and significantly different among tested groups (P< 0.05): Coarse (23.94 ± 2.52 nm) > Medium (17.00 ± 3.81 nm) > Fine (11.89 ± 1.68 nm). The contact angles of the Coarse group were the highest, followed by the Medium and the Fine groups. Increasing the surface roughness and hydrophobicity resulted in an increase of adhesion forces and early attachment (2 h and 4 h) ofS. mutanson the zirconia but no influence on the further development of biofilm (6 h~24 h). Our findings suggest that the surface roughness in nanoscale and hydrophobicity of zirconia had influence on theS. mutansinitial adhesion force and early attachment instead of whole stages of biofilm formation.
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Chuanyong
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Jinglin
authors.initials: J
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Jing
authors.identifier: 0000-0001-5398-4240
authors.initials: J
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Wei
authors.identifier: 0000-0002-2546-1853
authors.initials: W
authors.lastname: Li
mesh_terms: D000268:Adhesiveness / Q000187:drug effects; D001422:Bacterial Adhesion / Q000187:drug effects*; D050296:Microbial Viability / Q000187:drug effects; D018625:Microscopy, Atomic Force; D013194:Staining and Labeling; D013295:Streptococcus mutans / Q000166:cytology* / Q000187:drug effects / Q000648:ultrastructure; D015040:Zirconium / Q000494:pharmacology*
chemical_list: D015040:Zirconium; C028541:zirconium oxide