title: Biological Tissue Response to a New Formulation of a Silicone Based Endodontic Sealer.
abstract: Satisfactory biological behavior is a necessary requirement for clinical application of endodontic materials. In this study, the connective tissue responses to silicone (GuttaFlow 2), epoxy resin (AH Plus) and zinc oxide and eugenol (Endofill) based sealers were compared. Twelve Wistar rats had polyethylene tubes (four per animal) containing one of the tested sealers and empty tubes (negative control) implanted in their subcutaneous tissue. The tubes were randomly placed 2 cm from the spine and at least 2 cm apart from one another. Tissue samples with implants were processed for histological analysis after 7 or 60 days (n=6 animals per period). Inflammatory cells, fibrous condensation and abscess were scored according to their intensity. Friedman, followed by Dunn's post hoc, was used to compare sealers. Differences between the two experimental periods were verified using Mann-Witney U test (p<0.05). At 7 days, most of the histological parameters showed no significant differences amongst groups. Endofill group scored higher than the others for giant cells (o<0.05) and promoted a greater number of samples presenting abscess formation. GuttaFlow 2 tended to show a less intense inflammatory infiltrate compared to the other materials. At 60 days, there were no significant differences between groups in most of the histological parameters evaluated. However, it was observed that Endofill scored higher for macrophages (p<0.05) compared to the control group, and GuttaFlow 2 tended to present lower scores than the others for neutrophils and abscess. GuttaFlow 2 showed proper biological behavior and should be considered adequate for clinical practice.
authors.affiliation: Department of Conservative Dentistry, Dental School, UFRS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Flávia Emi Razera
authors.initials: FE
authors.lastname: Baldasso
authors.affiliation: Department of Conservative Dentistry, Dental School, UFRS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Patrícia Maria Poli
authors.initials: PM
authors.lastname: Kopper
authors.affiliation: Department of Stomatology, Dental School, UFSM - Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Renata Dornelles
authors.initials: RD
authors.lastname: Morgental
authors.affiliation: Warwick Medical School, University of Warwick, Warwick, United Kingdom.
authors.forename: Liviu
authors.initials: L
authors.lastname: Steier
authors.affiliation: Clinical Department, Dental School, PUCRS - Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazi.
authors.forename: José Antônio Poli de
authors.initials: JA
authors.lastname: Figueiredo
authors.affiliation: Department of Conservative Dentistry, Dental School, UFRS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
authors.forename: Roberta Kochenborger
authors.initials: RK
authors.lastname: Scarparo
mesh_terms: D000818:Animals; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D012387:Root Canal Filling Materials; D012828:Silicones
chemical_list: D012387:Root Canal Filling Materials; D012828:Silicones