title: NAD+as a Hydride Donor and Reductant.
abstract: Reduced nicotinamide adenine dinucleotide (NADH) can generate a ruthenium-hydride intermediate that catalyzes the reduction of O2to H2O2, which endows it with potent anticancer properties. A catalyst that could access a Ru-H intermediate using oxidized nicotinamide adenine dinucleotide (NAD+) as the H-source, however, could draw upon a supply of reducing equivalents 1000-fold more abundant than NADH, which would enable significantly greater H2O2production. Herein, it is demonstrated, using the reduction of ABTS•-to ABTS2-, that NAD+can function as a reductant. Mechanistic evidence is presented that suggests a Ru-H intermediate is formed via β-hydride elimination from a ribose subunit in NAD+. The insight gained from the heretofore unknown ability of NAD+to function as a reductant and H-donor may lead to undiscovered biological carbohydrate oxidation pathways and new chemotherapeutic strategies.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Htet
authors.affiliation: Center for Optical Materials Science and Engineering Technologies , Anderson, South Carolina 29625, United States.
authors.forename: Andrew G
authors.initials: AG
authors.lastname: Tennyson
mesh_terms: D015394:Molecular Structure; D009243:NAD / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D019163:Reducing Agents / Q000737:chemistry / Q000378:metabolism*
chemical_list: D019163:Reducing Agents; D009243:NAD