title: Hydroalcoholic extract of Brazilian red propolis exerts protective effects on acetic acid-induced ulcerative colitis in a rodent model.
abstract: Ulcerative colitis (UC) is a common intestinal inflammatory disease with an etiology that is not well understood. Although the anti-inflammatory and anti-oxidant effects of the hydroalcoholic extract of Brazilian red propolis (HERP) have been reported in various experimental models, its protective effect in models of UC have not been evaluated. The purpose of this study was to investigate the chemopreventive effect of hydroalcoholic extract of Brazilian red propolis (HERP) in acetic acid-induced colitis (AAIC) using a rodent model. The HERP was chemically characterised by HPLC/DAD analyses. Male rats were randomly assigned into four groups: sham, vehicle (with AAIC, treated with vehicle), P10 (with AAIC, treated with 10mg/kg HERP), and P100 (with AAIC, treated with 100mg/kg HERP). Treatments were performed for 7days, and colitis was induced on day seven. Animals were euthanized 24h after colitis induction and body weight, colon length, gross and histological scores, malondialdehyde (MDA) and myeloperoxidase (MPO) concentrations in colon tissue, and the immunohistochemical expression of inducible nitric oxide synthase (iNOS) were assessed. The major compounds found in HERP were liquiritigenin (68.8mg/g), formononetin (54.29mg/g), biochanin A (30.97mg/g), and daidzein (19.90mg/g). Rats treated with 10mg/kg HERP demonstrated significant decreases in MPO concentrations, gross and histological scores of tissue damage, and iNOS expression (p<0.05). Similarly, rats treated with 100mg/kg HERP demonstrated significant decreases in MPO levels (p<0.05) and histological scores of tissue damage (p<0.05). The results of this study indicate that oral administration of HERP attenuates AAIC in rats, which may be due to anti-inflammatory effects related to iNOS inhibition.
authors.affiliation: Tiradentes University, 300 Murilo Dantas Ave, Farolândia 49032-490 Aracaju, SE, Brazil.
authors.forename: Gislaine
authors.initials: G
authors.lastname: Barbosa Bezerra
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Luana
authors.initials: L
authors.lastname: de Menezes de Souza
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Adailma Santana
authors.initials: AS
authors.lastname: Dos Santos
authors.affiliation: Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Grace Kelly Melo
authors.initials: GK
authors.lastname: de Almeida
authors.affiliation: Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Marília Trindade Santana
authors.initials: MT
authors.lastname: Souza
authors.affiliation: Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Sandra Lauton
authors.initials: SL
authors.lastname: Santos
authors.affiliation: Department of Physiology, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Enilton
authors.initials: E
authors.lastname: Aparecido Camargo
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Bruno
authors.initials: B
authors.lastname: Dos Santos Lima
authors.affiliation: Department of Pharmacy, Federal University of Sergipe, Marechal Rondon Ave, Cidade Universitária, CEP 49100-000 São Cristóvão, SE, Brazil.
authors.forename: Adriano Antunes
authors.initials: AA
authors.lastname: de Souza Araújo
authors.affiliation: Tiradentes University, 300 Murilo Dantas Ave, Farolândia 49032-490 Aracaju, SE, Brazil; Research and Technology Institute (ITP), 300 Murilo Dantas Ave, Farolândia, 49032-490 Aracaju, SE, Brazil.
authors.forename: Juliana Cordeiro
authors.initials: JC
authors.lastname: Cardoso
authors.affiliation: Research and Technology Institute (ITP), 300 Murilo Dantas Ave, Farolândia, 49032-490 Aracaju, SE, Brazil.
authors.forename: Silvana Vieira Floresta
authors.initials: SV
authors.lastname: Gomes
authors.affiliation: Tiradentes University, 300 Murilo Dantas Ave, Farolândia 49032-490 Aracaju, SE, Brazil; Research and Technology Institute (ITP), 300 Murilo Dantas Ave, Farolândia, 49032-490 Aracaju, SE, Brazil.
authors.forename: Margarete Zanardo
authors.initials: MZ
authors.lastname: Gomes
authors.affiliation: Tiradentes University, 300 Murilo Dantas Ave, Farolândia 49032-490 Aracaju, SE, Brazil; Research and Technology Institute (ITP), 300 Murilo Dantas Ave, Farolândia, 49032-490 Aracaju, SE, Brazil. Electronic address: ricardo.patologia@uol.com.br.
authors.forename: Ricardo Luiz Cavalcanti
authors.initials: RL
authors.lastname: de Albuquerque
mesh_terms: D019342:Acetic Acid / Q000633:toxicity*; D000818:Animals; D005591:Chemical Fractionation; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000517:prevention & control*; D004305:Dose-Response Relationship, Drug; D008297:Male; D011429:Propolis / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar
chemical_list: D011429:Propolis; D019342:Acetic Acid
keywords: Anti-inflammatory agents; Antioxidants; Propolis; Ulcerative colitis; Wistar rats