title: Facile Synthesis of Mesocrystalline SnO2Nanorods on Reduced Graphene Oxide Sheets: An Appealing Multifunctional Affinity Probe for Sequential Enrichment of Endogenous Peptides and Phosphopeptides.
abstract: A novel multifunctional composite comprising mesocrystalline SnO2nanorods (NRs) vertically aligned on reduced graphene oxide (rGO) sheets was synthesized and developed for sequential capture of endogenous peptides and phosphopeptides. With the hydrophobicity of rGO and high affinity of SnO2nanorods, sequential enrichment of endogenous peptides and phosphopeptides could be easily achieved through a modulation of elution buffer. With this multifunctional nanomaterial, 36 peptides were observed from diluted bovine serum albumin (BSA) tryptic digest and 4 phosphopeptides could be selectively captured from β-casein digest. The detection limit of tryptic digest of β-casein was low to 4 × 10-10M, and the selectivity was up to 1:500 (molar ratio of β-casein and BSA digest). The effectiveness and robustness of rGO-SnO2NRs in a complex biological system was also confirmed by using human serum as a real sample. Our work is promising for small peptide enrichment and identification especially in complicated biological sample preparation, which also opens a new perspective in the design of multifunctional affinity probes for proteome or peptidome.
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University , Beijing, 100871, PR China.
authors.forename: Wen
authors.initials: W
authors.lastname: Ma
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Stable and Unstable Species, College of Chemistry, Peking University , Beijing 100871, PR China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Capital Normal University , Beijing 100048, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Li
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Stable and Unstable Species, College of Chemistry, Peking University , Beijing 100871, PR China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Chen
authors.affiliation: Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Stable and Unstable Species, College of Chemistry, Peking University , Beijing 100871, PR China.
authors.forename: Limin
authors.initials: L
authors.lastname: Qi
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University , Beijing, 100871, PR China.
authors.forename: Huwei
authors.initials: H
authors.lastname: Liu
authors.affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry, Peking University , Beijing, 100871, PR China.
authors.forename: Yu
authors.identifier: http://orcid.org/0000-0003-1542-0297
authors.initials: Y
authors.lastname: Bai
mesh_terms: D002364:Caseins; D002846:Chromatography, Affinity; D006108:Graphite; D006801:Humans; D043942:Nanotubes; D010087:Oxides; D010748:Phosphopeptides; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D017971:Tin Compounds
chemical_list: D002364:Caseins; D010087:Oxides; D010748:Phosphopeptides; D017971:Tin Compounds; D006108:Graphite; C045358:stannic oxide
keywords: endogenous peptides; mass spectrometry; multifunctional affinity probe; phosphopeptides; sequential enrichment