title: Automatic determination of coenzyme Q10 in food using cresyl violet encapsulated into magnetoliposomes.
abstract: A new type of magnetoliposomes (MLs), containing hydrophobic magnetic-gold nanoparticles and the long wavelength fluorophore cresyl violet, has been used for the determination of coenzyme Q10 (CoQ10). MLs were concentrated just before the detector, using a flow system and an external electromagnet device. The subsequent introduction of Triton X-100 and CoQ10 causes the MLs lysis and the cresyl violet oxidation, obtaining a decrease in the fluorescence signal. The dynamic range of the calibration graph was 0.03-0.50μmolL-1CoQ10, and the detection limit was 0.008μmolL-1. The precision (relative standard deviation) was in the range of 1.3-4.5%. The method showed a sampling frequency of 12h-1and was applied to the determination of CoQ10 in several food samples. The results were compared with those obtained using a previously described chromatographic method. Also, recovery values were in the range of 83.5-101.3%.
authors.affiliation: Analytical Chemistry Department, Institute of Fine Chemistry and Nanochemistry, Campus of Rabanales, Annex to Marie Curie Building, University of Córdoba, 14071 Córdoba, Spain. Electronic address: vanesa_ropi@hotmail.com.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Román-Pizarro
authors.affiliation: Analytical Chemistry Department, Institute of Fine Chemistry and Nanochemistry, Campus of Rabanales, Annex to Marie Curie Building, University of Córdoba, 14071 Córdoba, Spain. Electronic address: qa1feroj@uco.es.
authors.forename: Juan Manuel
authors.initials: JM
authors.lastname: Fernández-Romero
authors.affiliation: Analytical Chemistry Department, Institute of Fine Chemistry and Nanochemistry, Campus of Rabanales, Annex to Marie Curie Building, University of Córdoba, 14071 Córdoba, Spain. Electronic address: qa1gohea@uco.es.
authors.forename: Agustina
authors.initials: A
authors.lastname: Gómez-Hens
mesh_terms: D048588:Benzoxazines / Q000737:chemistry*; D002138:Calibration; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005504:Food Analysis; D006046:Gold / Q000737:chemistry; D057230:Limit of Detection; D008081:Liposomes / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry; D014451:Ubiquinone / Q000031:analogs & derivatives* / Q000737:chemistry
chemical_list: D048588:Benzoxazines; D004800:Enzymes, Immobilized; D008081:Liposomes; D014451:Ubiquinone; C028911:cresyl violet; D006046:Gold; C024989:coenzyme Q10
keywords: Coenzyme Q10; Fluorometric detection; Food analysis; Magnetoliposomes; On-flow micro-containers