title: Development of a heterologous enzyme-linked immunosorbent assay for the detection of clindamycin and lincomycin residues in edible animal tissues.
abstract: In this study, new clindamycin (CLIN) artificial antigens were prepared and used to produce broad-specificity monoclonal antibodies. Based on the as-produced mAbs, a heterologous ELISA was developed to detect CLIN and lincomycin (LIN) residues in edible animal tissues. The IC50values of the developed assay were 0.3ng/mL (CLIN) and 1.2ng/mL (LIN) in buffer, respectively. The detection limits were estimated to be 1.8μg/kg (CLIN) and 6.8μg/kg (LIN) in bovine, chicken, porcine and fish muscles. In the spike and recovery tests, the mean recovery rate ranged from 76% to 112% at different spiked levels, and the intra-/inter-assay coefficients of variation were in the range of 7.1% to 13.2%. This method was verified using LC-MS/MS with a correlation coefficient >0.97. The developed ELISA is therefore well suited for simultaneous determination of CLIN and LIN residues in bovine, chicken, porcine and fish muscles.
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Jie
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wu
authors.affiliation: Key Laboratory of Food Safety Risk Assessment, Ministry of Health, China National Center for Food Safety Risk Assessment, Beijing 100021, China.
authors.forename: Pengjie
authors.initials: P
authors.lastname: Luo
authors.affiliation: Technology Center of JiangXi Entry-Exit Inspection and Quarantine Bureau, Nanchang 330038, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Qu
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zou
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Feiyao
authors.initials: F
authors.lastname: Wu
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Xie
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China.
authors.forename: Can
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Physiology, School of Basic Medical Sciences, Shenzhen University Health Sciences Center, Shenzhen 518060, China. Electronic address: jiangwenxiao@szu.edu.cn.
authors.forename: Wenxiao
authors.initials: W
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D000911:Antibodies, Monoclonal / Q000032:analysis; D002417:Cattle; D002645:Chickens; D002981:Clindamycin / Q000032:analysis*; D004350:Drug Residues / Q000032:analysis; D004797:Enzyme-Linked Immunosorbent Assay; D005399:Fishes; D020128:Inhibitory Concentration 50; D057230:Limit of Detection; D008034:Lincomycin / Q000032:analysis*; D018482:Muscle, Skeletal / Q000737:chemistry; D011200:Poultry; D000069466:Red Meat / Q000032:analysis*; D017747:Seafood / Q000032:analysis*; D012680:Sensitivity and Specificity; D053719:Tandem Mass Spectrometry; D019155:Veterinary Drugs / Q000032:analysis
chemical_list: D000900:Anti-Bacterial Agents; D000911:Antibodies, Monoclonal; D019155:Veterinary Drugs; D002981:Clindamycin; D008034:Lincomycin
keywords: Antibody; Clindamycin; Food safety; Immunoassay; Lincomycin