title: Dopamine Modified Organic-Inorganic Hybrid Coating for Antimicrobial and Osteogenesis.
abstract: A hybrid coating composed of hydroxyapatite (HA), Ag nanoparticles (NPs), and chitosan (CS) was successfully prepared on a Ti substrate by a layer-by-layer assembly process. A polydopamine-assisted (PDA-assisted) coating showed a good bond with HA. Ag NPs were uniformly distributed into the hybrid coating through a solution method and ultraviolet light reduction. A CS nanofilm was deposited via spin-coating to control the release of Ag+from the hybrid coating. The results disclosed that the 3-layer CS coating could efficiently control the release of Ag+from the hybrid coating via the Fickian diffusion mechanism and that the PDA/HA/Ag/CS-1 coating exhibited antibacterial ratios of 63.0% and 51.8% against E. coli and S. aureus, respectively. Furthermore, the normal structure of E. coli was obviously destroyed by two types of Ag doped coatings. The cell viability assay showed that CS effectively reduced the cytotoxicity of the hybrid coating after a 7 day incubation. The hybrid coating presented high ALP activities at days 3 and 14. The results reveal that hybrid coatings can endow Ti implants with good antibacterial capability as well as cell viability and osteogenic activity.
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan 430062, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan 430062, China.
authors.forename: Xiangmei
authors.initials: X
authors.lastname: Liu
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan 430062, China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Division of Spine Surgery, Department of Orthopaedics & Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong , Pokfulam, Hong Kong, China.|Shenzhen Key Laboratory for Innovative Technology in Orthopaedic Trauma, The University of Hong Kong Shenzhen Hospital , 1 Haiyuan First Road, Futian Distract, Shenzhen, China.
authors.forename: K W K
authors.initials: KW
authors.lastname: Yeung
authors.affiliation: Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry of Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University , Wuhan 430062, China.
authors.forename: Shuilin
authors.initials: S
authors.lastname: Wu
mesh_terms: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D004298:Dopamine / Q000737:chemistry*; D017886:Durapatite; D004926:Escherichia coli; D010012:Osteogenesis; D012834:Silver; D013211:Staphylococcus aureus; D014025:Titanium
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D012834:Silver; D017886:Durapatite; D014025:Titanium; D004298:Dopamine
keywords: HA/Ag/CS hybrid coating; antibacterial; biocompatibility; poly(dopamine); release; titanium