title: Drebrin: A new oncofetal biomarker associated with prognosis of lung adenocarcinoma.
abstract: With the aim of searching for novel oncofetal tumor biomarkers of lung adenocarcinoma other than carcinoembryonic antigen (CEA) and alpha-fetoprotein (AFP), we developed a strategy involving monoclonal antibodies generated from embryonic tissue of miniature swine.
  
  
  
  Using immunohistochemistry, we selected suitable hybridoma clones that were reactive against swine fetal lung but not adult lung using tissue microarray loading of human normal lung, lung cancer, and fetal and adult swine tissues.
  
  
  
  The selected clones included several that were uniquely reactive against both swine fetal lung and human lung adenocarcinoma, and protein microarray revealed that the antigen they recognized was "drebrin" (DBN1). We then examined the association between the pattern of drebrin expression and the clinicopathological characteristics of lung adenocarcinoma using surgically resected samples of human lung adenocarcinoma. Two hundred formalin-fixed and paraffin-embedded tumor samples were immunostained for drebrin using clone B246, one of the clones that were reactive against drebrin. The cases were divided into those with strong (n=85) and weak (n=115) drebrin expression. In terms of disease-free survival, cases showing strong drebrin expression had a significantly poorer prognosis than those with weak drebrin expression (p=0.033).
  
  
  
  The present findings indicate that "drebrin" is a unique oncofetal protein that can be applied as a new biomarker of lung adenocarcinoma.
authors.affiliation: Doctoral Program in Biomedical Science, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan; Department of Respiratory Medicine, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Iyama
authors.affiliation: Department of Pathology, National Hospital Organization Mito Medical Center, Ibaraki, Japan.
authors.forename: Masao
authors.initials: M
authors.lastname: Ono
authors.affiliation: Doctoral Program in Biomedical Science, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan; Department of Pathology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Hitomi
authors.initials: H
authors.lastname: Kawai-Nakahara
authors.affiliation: Doctoral Program in Biomedical Science, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Ryan Edbert
authors.initials: RE
authors.lastname: Husni
authors.affiliation: Doctoral Program in Biomedical Science, Graduate School of Comprehensive Human Sciences, University of Tsukuba, Ibaraki, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Dai
authors.affiliation: Department of Respiratory Medicine, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Shiozawa
authors.affiliation: Department of Pathology, Hitachi General Hospital, Ibaraki, Japan.
authors.forename: Akiko
authors.initials: A
authors.lastname: Sakata
authors.affiliation: Department of Respiratory Medicine, Faculty of Life Sciences, Kumamoto University, Kumamoto, Japan.
authors.forename: Hirotsugu
authors.initials: H
authors.lastname: Kohrogi
authors.affiliation: Department of Pathology, Faculty of Medicine, University of Tsukuba, Ibaraki, Japan. Electronic address: nmasayuk@md.tsukuba.ac.jp.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Noguchi
mesh_terms: D000230:Adenocarcinoma / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D000077192:Adenocarcinoma of Lung; D000368:Aged; D000818:Animals; D014408:Biomarkers, Tumor / Q000096:biosynthesis*; D018572:Disease-Free Survival; D005260:Female; D006801:Humans; D006825:Hybridomas; D007150:Immunohistochemistry; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D009479:Neuropeptides / Q000096:biosynthesis*; D011379:Prognosis; D013552:Swine
chemical_list: D014408:Biomarkers, Tumor; D009479:Neuropeptides; C053386:drebrins
keywords: Biomarker; Drebrin; Lung adenocarcinoma; Oncofetal protein