title: Effect of temperature and seven storage media on human periodontal ligament fibroblast viability.
abstract: OBJECTIVE
  Natural resources, such as coconut water, propolis, and egg whites, have been examined as possible storage media for avulsed teeth. However, there is a lack of research focused on the efficacy of these three products together compared with Hank's balanced salt solution and milk. The aim of this study was to evaluate the capacity of seven storage media to maintain the viability of human periodontal ligament fibroblasts (PDLFs).
  
  
  METHODS
  PDLFs were kept at 5°C and 20°C, in skimmed milk (SMilk), whole milk (WMilk), recently prepared Hank's balanced salt solution (HBSS), Save-A-Tooth®system's HBSS (Save), natural coconut water (Coconut), Propolis, and egg white (Egg) for 3, 6, 24, 48, 72, 96, and 120 h, through the analysis of tetrazolium salt-based colorimetric (MTT) assay.
  
  
  RESULTS
  At 5°C, SMilk and WMilk were better than HBSS in maintaining cell viability, from 24 h onward. At 20°C, HBSS was the best storage medium at 96 and 120 h. At both temperatures, from 6 h onward, Coconut, Propolis and Egg were less effective than SMilk, WMilk, and HBSS. In general, the performance of Coconut, Propolis and Egg were not influenced by storage temperature. However, the lowest temperature undermined the effectiveness of HBSS from 24 h and favored SMilk and WMilk, from 96 and 48 h onward, respectively. Save and water were the worst storage media.
  
  
  CONCLUSIONS
  SMilk was the best storage medium, followed by WMilk and HBSS. Coconut, Propolis, and Egg can be indicated for the conservation of PDLF up to 3 h. The lower temperature (5°C) undermined the effectiveness of HBSS and favored SMilk and WMilk.
authors.affiliation: Department of Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.
authors.forename: Beatriz Dulcineia Mendes
authors.initials: BD
authors.lastname: de Souza
authors.affiliation: Department of Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.
authors.forename: Eduardo Antunes
authors.initials: EA
authors.lastname: Bortoluzzi
authors.affiliation: LICIFO, Faculty of Dentistry, University of Costa Rica.
authors.forename: Jessie
authors.initials: J
authors.lastname: Reyes-Carmona
authors.affiliation: Department of Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.
authors.forename: Luciane Geanini Pena
authors.initials: LG
authors.lastname: Dos Santos
authors.affiliation: Department of Pharmaceutical Sciences, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.
authors.forename: Claudia Maria de Oliveira
authors.initials: CM
authors.lastname: Simões
authors.affiliation: Department of Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.
authors.forename: Wilson Tadeu
authors.initials: WT
authors.lastname: Felippe
authors.affiliation: Department of Dentistry, Federal University of Santa Catarina, Florianopolis, Santa Catarina, Brazil.
authors.forename: Mara Cristina Santos
authors.initials: MC
authors.lastname: Felippe
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects*; D003059:Cocos; D004529:Egg White; D005347:Fibroblasts / Q000502:physiology*; D006801:Humans; D007552:Isotonic Solutions / Q000494:pharmacology; D008892:Milk; D019188:Organ Preservation Solutions / Q000494:pharmacology*; D010513:Periodontal Ligament / Q000166:cytology*; D011429:Propolis / Q000494:pharmacology; D013696:Temperature
chemical_list: D007552:Isotonic Solutions; D019188:Organ Preservation Solutions; D011429:Propolis
keywords: avulsion; coconut water; egg white; periodontal ligament fibroblasts; propolis; storage media