title: Circulating MicroRNA-21, MicroRNA-23a, and MicroRNA-125b as Biomarkers for Diagnosis and Prognosis of Burkitt Lymphoma in Children.
abstract: BACKGROUND The aim of this study was to investigate the diagnostic and prognostic value of microRNA (miRNA)-21, miRNA-23a, and miRNA-125b in Burkitt lymphoma (BL) in children. MATERIAL AND METHODS We recruited 41 children with BL for the case group, 56 children with lymph node inflammation for the positive control group, and 60 healthy children for the negative control group. Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was conducted for detection of circulating miRNA-21, miRNA-23a, and miRNA-125b. A receiver operating characteristic (ROC) curve was drawn to compare the diagnostic value of miRNA-21, miRNA-23a, and miRNA-125b. Kaplan-Meier method and log-rank test were used for prognostic analyses. RESULTS MiRNA-21 and miRNA-23a had significantly higher expression in cases than in positive and negative controls (all P<0.05). Overexpression of miRNA-21 and miRNA-23a were associated with staging, WBC, upregulated serum lactate dehydrogenase (LDH) level, presence of lymphoma size ≥6 cm, and cluster of differentiation 10 (CD10) expression, while miRNA-125b expression had an association with staging and upregulated serum LDH level (both P<0.05). ROC curves of miRNA-21, miRNA-23a, and miRNA-125b presented an area under curve (AUC) of 0.759, 0.853 and 0.615, respectively. MiRNA-21 and miRNA-23a in combination had an AUC of 0.869. After treatment, both miRNA-21 and miRNA-23a expression were significantly decreased (both P<0.05). Advanced clinical stage, upregulated LDH, and lymphoma size of ³6 cm were related to low complete remission rate (all P<0.05). CONCLUSIONS Patients with high expression of miRNA-21 and miRNA-23a had significantly lower complete remission rates and survival rates than those with low expression. Expression of miRNA-21 and miRNA-23a may serve as useful diagnostic and prognostic biomarkers in children with BL.
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Fudan University, Shanghai, China (mainland).
authors.forename: Jun
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Fudan University, Shanghai, China (mainland).
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Zhai
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Fudan University, Shanghai, China (mainland).
authors.forename: Hong-Sheng
authors.initials: HS
authors.lastname: Wang
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Fudan University, Shanghai, China (mainland).
authors.forename: Xiao-Wen
authors.initials: XW
authors.lastname: Qian
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Fudan University, Shanghai, China (mainland).
authors.forename: Hui
authors.initials: H
authors.lastname: Miao
authors.affiliation: Department of Hematology and Oncology, Children's Hospital of Fudan University, Shanghai, China (mainland).
authors.forename: Xiao-Hua
authors.initials: XH
authors.lastname: Zhu
mesh_terms: D000293:Adolescent; D014408:Biomarkers, Tumor / Q000097:blood; D002051:Burkitt Lymphoma / Q000097:blood / Q000175:diagnosis* / Q000235:genetics; D016022:Case-Control Studies; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000235:genetics; D011379:Prognosis; D012372:ROC Curve; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; C531888:MIRN125 microRNA, human; C524940:MIRN21 microRNA, human; C552430:MIRN23a microRNA, human; D035683:MicroRNAs