title: A hamster model for Marburg virus infection accurately recapitulates Marburg hemorrhagic fever.
abstract: Marburg virus (MARV), a close relative of Ebola virus, is the causative agent of a severe human disease known as Marburg hemorrhagic fever (MHF). No licensed vaccine or therapeutic exists to treat MHF, and MARV is therefore classified as a Tier 1 select agent and a category A bioterrorism agent. In order to develop countermeasures against this severe disease, animal models that accurately recapitulate human disease are required. Here we describe the development of a novel, uniformly lethal Syrian golden hamster model of MHF using a hamster-adapted MARV variant Angola. Remarkably, this model displayed almost all of the clinical features of MHF seen in humans and non-human primates, including coagulation abnormalities, hemorrhagic manifestations, petechial rash, and a severely dysregulated immune response. This MHF hamster model represents a powerful tool for further dissecting MARV pathogenesis and accelerating the development of effective medical countermeasures against human MHF.
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Andrea
authors.initials: A
authors.lastname: Marzi
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Logan
authors.initials: L
authors.lastname: Banadyga
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Elaine
authors.initials: E
authors.lastname: Haddock
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Tina
authors.initials: T
authors.lastname: Thomas
authors.affiliation: Bioinformatics and Computational Biosciences Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
authors.forename: Kui
authors.initials: K
authors.lastname: Shen
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Eva J
authors.initials: EJ
authors.lastname: Horne
authors.affiliation: Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Dana P
authors.initials: DP
authors.lastname: Scott
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Heinz
authors.initials: H
authors.lastname: Feldmann
authors.affiliation: Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, MT 59840, USA.
authors.forename: Hideki
authors.initials: H
authors.lastname: Ebihara
mesh_terms: D000818:Animals; D001778:Blood Coagulation Disorders / Q000209:etiology; D002522:Chlorocebus aethiops; D006224:Cricetinae; D016207:Cytokines / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D005340:Fibrinogen / Q000032:analysis; D006470:Hemorrhage / Q000209:etiology; D007113:Immunity, Innate; D053208:Kaplan-Meier Estimate; D008099:Liver / Q000473:pathology; D008379:Marburg Virus Disease / Q000276:immunology / Q000401:mortality / Q000473:pathology* / Q000821:virology; D029024:Marburgvirus / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D009154:Mutation; D010314:Partial Thromboplastin Time; D011517:Prothrombin Time; D012367:RNA, Viral / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D013154:Spleen / Q000473:pathology; D014709:Vero Cells
chemical_list: D016207:Cytokines; D012367:RNA, Viral; D005340:Fibrinogen