title: MicroRNA-30e reduces cell growth and enhances drug sensitivity to gefitinib in lung carcinoma.
abstract: MicroRNAs (miRNAs) play critical roles in variousbiological processes,including malignancy. Here, we demonstrated that miR-30e levels were markedly reduced in human lung carcinoma specimens in comparisonwith adjacent normal tissues.In addition, miR-30eamounts were starkly lower in the resistant PC9/gefitinib (PC9G) cancer cells compared with PC9 cells. Meanwhile, miR-30eoverexpression inPC9G cells resulted in reduced cell proliferation and migration,reversing drug resistance to gefitinib.Conversely,miR-30e silencing in PC9 cells increased proliferation as well as migration, and conferred resistance to gefitinib.Moreover, HOXA1, which was identified asa new miR-30etarget, plays important roles in regulating cell fate, early developmental patterns and organogenesis.Importantly, miR-30ealso inhibited PC9G growth in vivo. Taken together, these findings demonstrated that miR-30eshould be considered a tumor suppressor miRNA, which could be used in treatinghuman lung cancer.
authors.affiliation: Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Ning
authors.affiliation: Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Lu
authors.affiliation: Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Chen
authors.affiliation: Institute of Medcine, University of Zhengzhou, Henan Province, 450000, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cai
authors.affiliation: Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
authors.forename: Ting-Hua
authors.initials: TH
authors.lastname: Cao
authors.affiliation: Department of Oncology, The First People's Hospital of Wujiang District, Suzhou, 215200, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China.|Collaborative Innovation Center for Cancer Medicine, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Yong-Qian
authors.initials: YQ
authors.lastname: Shu
authors.affiliation: Department of Oncology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, 210029, China.|Collaborative Innovation Center for Cancer Medicine, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Shen
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage* / Q000494:pharmacology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D015536:Down-Regulation; D019008:Drug Resistance, Neoplasm; D000077156:Gefitinib; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D018398:Homeodomain Proteins / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics / Q000473:pathology*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D009368:Neoplasm Transplantation; D011799:Quinazolines / Q000008:administration & dosage* / Q000494:pharmacology; D014157:Transcription Factors / Q000235:genetics*; D000099261:Homeobox A1 Protein
chemical_list: D000970:Antineoplastic Agents; D000077156:Gefitinib; D018398:Homeodomain Proteins; D035683:MicroRNAs; D011799:Quinazolines; D014157:Transcription Factors; D000099261:Homeobox A1 Protein; C546086:MIRN30b microRNA, human
keywords: HOXA1; chemosensitivity; gefitinib; lung cancer; miRNA-30e