title: Application of acellular intima from porcine thoracic aorta in full-thickness skin wound healing in a rat model.
abstract: The tunica intima of aorta is made up of one layer of smooth endothelium and basement membrane. The basement membrane is rich in extracellular matrix (ECM) molecules, including collagen, glycosaminoglycans (e.g., heparan sulfate), proteoglycans (e.g., perlecan), and glycoproteins. All or most of these components are involved in wound healing process. In this work, we determined whether the acellular intima from porcine thoracic aorta can be a new kind of xenograft to repair the skin-wound surface in a rat model. Acellular intima xenografts (AIX) were prepared from tunica intima, and then the swelling ratio, moisture content ratio, water retention ratio, degradation rate, and water vapor transmission rate of the materials were measured. Prothrombin time test was applied to assess its hemostatic property in vitro, in vitro cell experiment was used to test its cellular biocompatibility, and animal experiment was used to evaluate its effect on wound healing. Results showed that AIX, with some reasonable treatments, has good hemostatic property, cell biocompatibility, and histocompatibility. AIX can also promote angiogenesis in the healing process and thus accelerate comprehensive healing, thereby confirming supporting its functionality and excellent application potential in wound healing.
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Haitian
authors.initials: H
authors.lastname: Fu
authors.affiliation: Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Liping
authors.initials: L
authors.lastname: Teng
authors.affiliation: Wuxi No. 4 People's Hospital, Wuxi 214062, PR China.
authors.forename: RuiZhen
authors.initials: R
authors.lastname: Bai
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; Wuxi Medical School, Jiangnan University, Wuxi 214122, PR China.
authors.forename: Chao
authors.initials: C
authors.lastname: Deng
authors.affiliation: Wuxi No. 3 People's Hospital, Wuxi 214041, PR China.
authors.forename: Guozhong
authors.initials: G
authors.lastname: Lv
authors.affiliation: Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, PR China; School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, PR China. Electronic address: jhchenwhut@126.com.
authors.forename: Jinghua
authors.initials: J
authors.lastname: Chen
mesh_terms: D000818:Animals; D001013:Aorta, Thoracic / Q000737:chemistry*; D001705:Bioprosthesis; D005109:Extracellular Matrix / Q000737:chemistry*; D006801:Humans; D008297:Male; D008422:Materials Testing; D051379:Mice; D041681:NIH 3T3 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D017768:Skin, Artificial; D013552:Swine; D017539:Tunica Intima / Q000737:chemistry*; D014945:Wound Healing
keywords: Acellular tunica intima; Porcine thoracic aorta; Wound healing; Xenografts