title: In vivo MEMRI characterization of brain metastases using a 3D Look-Locker T1-mapping sequence.
abstract: Although MEMRI (Manganese Enhanced MRI) informations were obtained on primary tumors in small animals, MEMRI data on metastases are lacking. Thus, our goal was to determine if 3D Look-Locker T1 mapping was an efficient method to evaluate Mn ions transport in brain metastases in vivo. The high spatial resolution in 3D (156 × 156 × 218 μm) of the sequence enabled to detect metastases of 0.3 mm3. In parallel, the T1 quantitation enabled to distinguish three populations of MDA-MB-231 derived brain metastases after MnCl2 intravenous injection: one with a healthy blood-tumor barrier that did not internalize Mn2+ions, and two others, which T1 shortened drastically by 54.2% or 24%. Subsequent scans of the mice, enabled by the fast acquisition (23 min), demonstrated that these T1 reached back their pre-injection values in 24 h. Contrarily to metastases, the T1 of U87-MG glioma remained 26.2% shorter for one week. In vitro results supported the involvement of the Transient Receptor Potential channels and the Calcium-Sensing Receptor in the uptake and efflux of Mn2+ions, respectively. This study highlights the ability of the 3D Look-Locker T1 mapping sequence to study heterogeneities (i) amongst brain metastases and (ii) between metastases and glioma regarding Mn transport.
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR5536, CNRS/Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Charles R
authors.initials: CR
authors.lastname: Castets
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR5536, CNRS/Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Néha
authors.initials: N
authors.lastname: Koonjoo
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR5536, CNRS/Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Andreea
authors.initials: A
authors.lastname: Hertanu
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR5536, CNRS/Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Voisin
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR5536, CNRS/Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Franconi
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR5536, CNRS/Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Sylvain
authors.initials: S
authors.lastname: Miraux
authors.affiliation: Centre de Résonance Magnétique des Systèmes Biologiques, UMR5536, CNRS/Université de Bordeaux, 146 rue Léo Saignat, 33076 Bordeaux, France.
authors.forename: Emeline J
authors.initials: EJ
authors.lastname: Ribot
mesh_terms: D000818:Animals; D001932:Brain Neoplasms / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D002712:Chlorides / Q000378:metabolism; D003287:Contrast Media / Q000378:metabolism; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods; D007090:Image Interpretation, Computer-Assisted / Q000379:methods; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008345:Manganese / Q000378:metabolism; D017895:Manganese Compounds / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D015203:Reproducibility of Results
chemical_list: D002712:Chlorides; D003287:Contrast Media; D017895:Manganese Compounds; D008345:Manganese; C025340:manganese chloride