title: Kif2a regulates spindle organization and cell cycle progression in meiotic oocytes.
abstract: Kif2a is a member of the Kinesin-13 microtubule depolymerases. Here, we report the expression, subcellular localization and functions of Kif2a during mouse oocyte meiotic maturation. Immunoblotting analysis showed that Kif2a was gradually increased form GV to the M I stages, and then decreased slightly at the M II stage. Confocal microscopy identified that Kif2a localized to the meiotic spindle, especially concentrated at the spindle poles and inner centromeres in metaphase and translocated to the midbody at telophase. Kif2a depletion by siRNA microinjection generated severely defective spindles and misaligned chromosomes, reduced microtubule depolymerization, which led to significant pro-M I/M Iarrest and failure of first polar body (PB1) extrusion. Kif2a-depleted oocytes were also defective in spindle pole localization of γ-tubulin and showed spindle assembly checkpoint (SAC) protein Bub3 at the kinetochores even after 10 hr extended culture. These results demonstrate that Kif2a may act as a microtubule depolymerase, regulating microtubule dynamics, spindle assembly and chromosome congression, and thus cell cycle progression during mouse oocyte meiotic maturation.
authors.affiliation: Center for Reproductive Medicine; Department of Ob/Gy; Nanfang Hospital; Southern Medical University, Guangzhou City, 510515 China.
authors.forename: Zi-Yun
authors.initials: ZY
authors.lastname: Yi
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Xue-Shan
authors.initials: XS
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Zhao-Yang
authors.initials: ZY
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Tie-Gang
authors.initials: TG
authors.lastname: Meng
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Ouyang
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.affiliation: State Key Laboratory of Stem Cells and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101 China.
authors.forename: Qing-Yuan
authors.initials: QY
authors.lastname: Sun
authors.affiliation: Center for Reproductive Medicine; Department of Ob/Gy; Nanfang Hospital; Southern Medical University, Guangzhou City, 510515 China.
authors.forename: Song
authors.initials: S
authors.lastname: Quan
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D020090:Chromosome Segregation / Q000187:drug effects; D033481:Chromosomes, Mammalian / Q000378:metabolism; D005260:Female; D055785:Gene Knockdown Techniques; D016547:Kinesins / Q000378:metabolism*; D059566:M Phase Cell Cycle Checkpoints / Q000187:drug effects; D008540:Meiosis / Q000187:drug effects; D008813:Mice, Inbred ICR; D015739:Nocodazole / Q000494:pharmacology; D009865:Oocytes / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D017239:Paclitaxel / Q000494:pharmacology; D059705:Polar Bodies / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism*; D013347:Subcellular Fractions / Q000187:drug effects / Q000378:metabolism; D014404:Tubulin / Q000378:metabolism
chemical_list: D012097:Repressor Proteins; D014404:Tubulin; C477238:KIF2A protein, mouse; D016547:Kinesins; D017239:Paclitaxel; D015739:Nocodazole