title: Adhesiveness of opportunistic Staphylococcus aureus to materials used in dental office: In vitro study.
abstract: Staphylococcus aureus (S. aureus) is one of several opportunistic microbial pathogens associated with many healthcare problems. In the present study, S. aureus was assessed for its biofilm-forming ability on materials routinely used in dental offices, including stainless steel (SS), polyethylene (PE), and polyvinyl chloride (PVC). Materials that were tested were characterized for roughness (Ra) and surface free energy (SFE). The adhesion forces exerted by S. aureus to each substratum were investigated using atomic force microscopy (AFM), and biofilm formation was quantitatively assessed by crystal violet staining assay. AFM measurements demonstrated that the strongest adhesion forces (20 nN) were exerted on the PE surfaces (P < 0.05) and depended more on Ra. In addition, the results of biofilm formation capability indicated that S. aureus exhibited more affinity to SS materials when compared to the other materials (P < 0.05). This ability of biofilm formation seems to be more correlated to SFE (R = 0.65). Hence, control of the surface properties of materials used in dental practices is of crucial importance for preventing biofilm formation on dental materials to be used for patients' dental care.
authors.affiliation: Laboratory of Infectious Diseases and Biological Agents (LR99ES27), Faculty of Pharmacy, Monastir University, Monastir, Tunisia. Electronic address: abderrahmen_merghni@yahoo.fr.
authors.forename: Abderrahmen
authors.initials: A
authors.lastname: Merghni
authors.affiliation: Laboratory of Analysis, Treatment and Valorization of Environmental Pollutants and Products (LR01ES16), Faculty of Pharmacy, Monastir University, Monastir, Tunisia.
authors.forename: Karima
authors.initials: K
authors.lastname: Bekir
authors.affiliation: Université d'Artois, EA 7394, Institut Charles VIOLLETTE, Lens, F-62300, France; ISA Lille, EA 7394, Institut Charles VIOLLETTE, Lille, F-59000, France; Ulco, EA 7394, Institut Charles VIOLLETTE, Boulogne sur Mer, F-62200, France; Université de Lille, EA 7394, Institut Charles VIOLLETTE, Lille, F-59000, France.
authors.forename: Yassine
authors.initials: Y
authors.lastname: Kadmi
authors.affiliation: Orthodontic Department of Monastir Dental Clinic, Laboratory of Oral Health and Orofacial Rehabilitation (LR12ES11), Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Dallel
authors.affiliation: BioImaging Center Lille-FR3642, Lille, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Janel
authors.affiliation: BioImaging Center Lille-FR3642, Lille, France.
authors.forename: Simone
authors.initials: S
authors.lastname: Bovio
authors.affiliation: BioImaging Center Lille-FR3642, Lille, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Barois
authors.affiliation: BioImaging Center Lille-FR3642, Lille, France; Cellular Microbiology and Physics of Infection Group - Center of Infection and Immunity of Lille: CNRS UMR8204, INSERM U1019, Institut Pasteur de Lille, Lille Regional University Hospital Centre, Lille University, France.
authors.forename: Frank
authors.initials: F
authors.lastname: Lafont
authors.affiliation: Laboratory of Infectious Diseases and Biological Agents (LR99ES27), Faculty of Pharmacy, Monastir University, Monastir, Tunisia; Laboratory of Microbiology, University Hospital of Fattouma Bourguiba, Monastir, Tunisia.
authors.forename: Maha
authors.initials: M
authors.lastname: Mastouri
mesh_terms: D001422:Bacterial Adhesion; D018441:Biofilms; D003428:Cross Infection; D003770:Dental Offices; D006801:Humans; D018625:Microscopy, Atomic Force; D009894:Opportunistic Infections; D013203:Staphylococcal Infections / Q000382:microbiology*; D013211:Staphylococcus aureus / Q000502:physiology* / Q000648:ultrastructure; D013499:Surface Properties
keywords: Adhesion forces; Biofilm; Inert materials; Staphylococcus aureus; Surface characteristics