title: Establishment of 3D culture and induction of osteogenic differentiation of pre-osteoblasts using wet-collected aligned scaffolds.
abstract: Aligned fibrous scaffolds have attracted much interest in bone tissue engineering, because they are supposed to induce osteogenic differentiation. For the first time, aligned silk fibroin nanofibres were loosely packed using a novel wet-collection electrospinning method. Moreover, three-dimensional (3D) culture of MC3T3-E1 pre-osteoblasts was established on these fibrous scaffolds. Physicochemical properties of the scaffolds and the behaviour of MC3T3-E1 pre-osteoblasts on the scaffolds were analysed and compared with scaffolds obtained using traditional method. Ethanol bath improved the uniformity and alignment of the fibres and increased the thickness and porosity of the scaffolds. Structures of the fibres were well maintained after immediate crosslinking in ethanol. Cells on the wet-collected scaffolds exhibited more ordered arrangement and elongated morphology as well as faster and deeper infiltration. The ordered infiltration resulted in the establishment of the 3D culture of cells, promoting proliferation and osteogenic differentiation of the pre-osteoblasts. Thus, the wet-collected aligned scaffolds with improved topographical and physicochemical properties presents significant potential application in bone regeneration.
authors.affiliation: Hubei-MOSTKLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China; Chongqing Affiliated Hospital of Stomatology, Chongqing University of Medical Sciences, Chongqing 400015, China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Ding
authors.affiliation: Wuhan National Laboratory for Optoelectronics, School of Optical and Electronic Information, Huazhong University of Science and Technology, Wuhan 430074, China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Hubei-MOSTKLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Hubei-MOSTKLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Song
authors.affiliation: Hubei-MOSTKLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Yin
authors.affiliation: Hubei-MOSTKLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Yanru
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Hubei-MOSTKLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China.
authors.forename: Qiyi
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Hubei-MOSTKLOS & KLOBM, School and Hospital of Stomatology, Wuhan University, Wuhan 430079, China. Electronic address: wangjwei@hotmail.com.
authors.forename: Jiawei
authors.initials: J
authors.lastname: Wang
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002454:Cell Differentiation; D002460:Cell Line; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D010012:Osteogenesis; D047011:Silk / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D047011:Silk
keywords: 3D culture; Bone tissue regeneration; Electrospinning; Ethanol bath; Fibre alignment