title: Surface biomimetic modification with laminin-loaded heparin/poly-l-lysine nanoparticles for improving the biocompatibility.
abstract: Late thrombus and restenosis caused by delayed endothelialization and insufficient biocompatibility of polymer coating continue to be the greatest limitations of drug-eluting stents. In this study, based on the specific structure of vascular basement membrane, a novel biomimetic nano-coating was constructed by incorporating laminin into electrostatic-assembled heparin/poly-l-lysine nanoparticles. Alteration of heparin and poly-l-lysine concentration ratio in a certain range has no significantly influence nanoparticle size, uniformity and stability, but may affect the chemical property and subsequently the binding efficiency to dopamine-coated titanium surface. By use of this feature, four different nanoparticles were synthesized and immobilized on titanium surface for creating gradient nanoparticle binding density. According to in vitro biocompatibility evaluation, the nanoparticle modified surfaces were found to effectively block coagulation pathway and reduce thrombosis formation. Moreover, NP10L and NP15L modified surface with relatively low heparin exposing density (4.9 to 7.1μg/cm2) showed beneficial effect in selective promoting EPCs and ECs proliferation, as well as stimulating cell migration and NO synthesis.
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an, China. Electronic address: 11140021@hyit.edu.cn.
authors.forename: Tao
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Geriatrics, The Affiliated Huai'an Hospital of Xuzhou Medical College, Huai'an, China.
authors.forename: Youdong
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
authors.forename: Jianying
authors.initials: J
authors.lastname: Tan
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an, China.
authors.forename: Shihui
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Lab. of Advanced Technology for Materials of Chinese Education Ministry, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu, China.
authors.forename: Junying
authors.initials: J
authors.lastname: Chen
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Guo
authors.affiliation: Jiangsu Provincial Key Laboratory for Interventional Medical Devices, Huaiyin Institute of Technology, Huai'an, China.
authors.forename: Changjiang
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Geriatrics, The Affiliated Huai'an Hospital of Xuzhou Medical College, Huai'an, China. Electronic address: xial_li@qq.com.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry / Q000494:pharmacology; D001792:Blood Platelets / Q000378:metabolism*; D006493:Heparin / Q000737:chemistry / Q000494:pharmacology; D006801:Humans; D007797:Laminin / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D053758:Nanoparticles / Q000737:chemistry*; D010973:Platelet Adhesiveness / Q000187:drug effects; D011107:Polylysine / Q000737:chemistry / Q000494:pharmacology; D013499:Surface Properties
chemical_list: D007797:Laminin; D011107:Polylysine; D006493:Heparin
keywords: Biomimetic; Endothelialization; Hemocompatibility; Heparin; Nanoparticles