title: Preparation, physicochemical properties and biocompatibility of PBLG/PLGA/bioglass composite scaffolds.
abstract: In this study, novel poly(γ-benzyl l-glutamate)/poly(lactic-co-glycolic acid)/bioglass (PBLG/PLGA/BG) composite scaffolds with different weight ratios were fabricated using a negative NaCl-templating method. The morphology, compression modulus and degradation kinetics of the scaffolds were characterized. The results showed that the PBLG/PLGA/BG composite scaffolds with a weight ratio of 5:5:1, namely PBLG5PLGA5BG composite scaffolds, displayed a pore size range of 50-500μm, high compressive modulus (566.6±8.8kPa), suitable glass transition temperature (46.8±0.2°C) and low degradation rate (>8weeks). The in vitro biocompatibility of the scaffolds was evaluated with MC3T3-E1 cells by live-dead staining, MTT and ALP activity assays. The obtained results indicated that the PBLG5PLGA5BG composite scaffolds were more conducive to the adhesion, proliferation and osteoblastic differentiation of MC3T3-E1 cells than PBLG and PBLG/PLGA composite scaffolds. The in vivo biocompatibility of the scaffolds was evaluated in both SD rat subcutaneous model and rabbit tibia defect model. The results of H&E, Masson's trichrome and CD34 staining assays demonstrated that the PBLG5PLGA5BG composite scaffolds allowed the ingrowth of tissue and microvessels more effectively than PBLG/PLGA composite scaffolds. The results of digital radiography confirmed that the PBLG5PLGA5BG composite scaffolds significantly improved in vivo osteogenesis. Collectively, the PBLG5PLGA5BG composite scaffolds could be a promising candidate for tissue engineering applications.
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Cui
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China. Electronic address: jmqian@mail.xjtu.edu.cn.
authors.forename: Junmin
authors.initials: J
authors.lastname: Qian
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Jinlei
authors.initials: J
authors.lastname: Wang
authors.affiliation: The Orthopaedic Department, XiJing Hospital Affiliated to the Fourth Military Medical University, Xi'an 710032, China.
authors.forename: Chuanlei
authors.initials: C
authors.lastname: Ji
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
authors.forename: Hongjie
authors.initials: H
authors.lastname: Wang
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002516:Ceramics / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D019344:Lactic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011099:Polyglutamic Acid / Q000031:analogs & derivatives* / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D011100:Polyglycolic Acid / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C022107:Bioglass; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; C003172:poly-gamma-benzyl-L-glutamate; D011099:Polyglutamic Acid; D011100:Polyglycolic Acid; D019344:Lactic Acid
keywords: Bioglass; Bone tissue engineering; Foam-like structure; Poly(lactic-co-glycolic acid); Poly(γ-benzyl l-glutamate); Scaffold