title: Interaction of Extracellular Vesicles with Endothelial Cells Under Physiological Flow Conditions.
abstract: In the last few years it has become clear that, in addition to soluble molecules such as growth factors and cytokines, cells use extracellular vesicles (EVs) for intercellular communication. For example, EVs derived from cancer cells interact with endothelial cells, thereby affecting angiogenesis and metastasis, two essential processes in tumor progression. In most experiments, the interaction of EVs with target cells is investigated under static conditions. However the use of dynamic flow conditions is considered more relevant, especially when studying EV uptake by endothelial cells. Here, we describe the use of a perfusion system to investigate the interaction of (tumor) EVs with endothelial cells under dynamic flow conditions.
authors.affiliation: Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, 3584, CX, The Netherlands.
authors.forename: Susan M
authors.initials: SM
authors.lastname: van Dommelen
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Margaret
authors.initials: M
authors.lastname: Fish
authors.affiliation: Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, 3584, CX, The Netherlands.
authors.forename: Arjan D
authors.initials: AD
authors.lastname: Barendrecht
authors.affiliation: Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, 3584, CX, The Netherlands.
authors.forename: Raymond M
authors.initials: RM
authors.lastname: Schiffelers
authors.affiliation: Department of Chemical Engineering, University of Michigan, Ann Arbor, MI, USA.
authors.forename: Omolola
authors.initials: O
authors.lastname: Eniola-Adefeso
authors.affiliation: Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Heidelberglaan 100, Utrecht, 3584, CX, The Netherlands. pvader@umcutrecht.nl.
authors.forename: Pieter
authors.initials: P
authors.lastname: Vader
mesh_terms: D002450:Cell Communication; D002458:Cell Fractionation / Q000379:methods; D002460:Cell Line; D017077:Culture Media, Conditioned; D042783:Endothelial Cells / Q000378:metabolism*; D000067128:Extracellular Vesicles / Q000378:metabolism*; D006801:Humans; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods
chemical_list: D017077:Culture Media, Conditioned
keywords: Binding; Endothelial cells; Extracellular vesicles; Physiological flow; Targeting; Uptake