title: MicroRNA expression analysis in the liver of high fat diet-induced obese mice.
abstract: A previous study indicated a causal link between certain miRNAs induced by obesity and the development of hepatic insulin resistance and type 2 diabetes. Here we provide accompanying data collected using Affymetrix GeneChip miRNAs microarrays to identify the changes in miRNAs expression in the liver of mice fed a high fat diet (HFD). Differentially expressed microRNA analyses in the liver of the HFD-fed mice revealed a range of upregulated (>1.5-fold) or downregulated (<0.5-fold) miRNAs. Among those upregulated miRNAs,in silicotarget analysis, such as TargetScan, PicTar, and miRWalk, identified miRNAs with the putative binding sites on the 3'UTRs ofINSRand/orIRS-1. Interpretation of the data and further extensive insights into the implication of miRNAs, particularly miR-15b, in hepatic insulin resistance can be found in "Obesity-induced miR-15b is linked causally to the development of insulin resistance through the repression of the insulin receptor in hepatocytes." (W.M. Yang, H.J. Jeong, S.W. Park, W. Lee, 2015)[1].
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, South Korea.
authors.forename: Won-Mo
authors.initials: WM
authors.lastname: Yang
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, South Korea.
authors.forename: Kyung-Ho
authors.initials: KH
authors.lastname: Min
authors.affiliation: Department of Biochemistry, Dongguk University College of Medicine, Gyeongju 780-714, South Korea; Endocrine Channelopathy, Channelopathy Research Center, Dongguk University College of Medicine, Goyang 410-773, South Korea.
authors.forename: Wan
authors.initials: W
authors.lastname: Lee
keywords: High fat diet; Liver; Mice; MicroRNAs; Obesity; Saturated fatty acids