title: The Caatinga endemic Manilkara rufula possesses remarkable activity against Trichomonas vaginalis and Tritrichomonas foetus.
abstract: Tritrichomonas foetus infects the bovine urogenital tract, causing bovine trichomoniasis. Significant economic losses may occur due to infertility and abortion among cattle. Trichomonas vaginalis is the causative agent of trichomoniasis; the most common but overlooked non-viral sexually transmitted disease worldwide. Human and bovine trichomoniasis present treatment restrictions and efforts to identify new alternatives are essential. The present study evaluated the anti-trichomonads activities of seven fractions from northwest endemic plant Manilkara rufula. Flavonoids and condensed tannins were identified from these fractions by LC-DAD-MS/MS and MALDI-MS/MS. Altogether, the results demonstrated for the first time the structural description of tannins from leaves of M. rufula and the relation of these compounds with anti-T. vaginalis and anti-T. foetus activities. Overall, this report reveals the potential of M. rufula fractions against both parasites and shows new alternatives to treat the infection caused by trichomonads.
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Programa de Pós-graduação em Ciências Biológicas, Universidade Federal do Pampa, São Gabriel, RS, Brazil. Electronic address: patriciasbrum@yahoo.com.br.
authors.forename: Patrícia
authors.initials: P
authors.lastname: de Brum Vieira
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. Electronic address: nicolaslfsilva@gmail.com.
authors.forename: Nícolas Luiz
authors.initials: NL
authors.lastname: Feijó Silva
authors.affiliation: Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Universidade Federal de Mato Grosso do Sul (UFMS), Campo Grande, MS, Brazil; Núcleo de Pesquisas em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: denisebrentan@gmail.com.
authors.forename: Denise Brentan
authors.initials: DB
authors.lastname: Silva
authors.affiliation: Núcleo de Pesquisas em Produtos Naturais e Sintéticos (NPPNS), Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic address: npelopes@fcfrp.usp.br.
authors.forename: Norberto Peporine
authors.initials: NP
authors.lastname: Lopes
authors.affiliation: Centro de Ciências Biológicas e Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brazil. Electronic address: agsilva@live.com.
authors.forename: Alexandre Gomes
authors.initials: AG
authors.lastname: da Silva
authors.affiliation: Centro de Ciências Biológicas e Departamento de Bioquímica, Universidade Federal de Pernambuco, Recife, PE, Brazil. Electronic address: marcia.vanusa@ufpe.br.
authors.forename: Márcia Vanusa
authors.initials: MV
authors.lastname: da Silva
authors.affiliation: Department of Natural Products, Plant Biology and Soil Science, Faculty of Pharmacy, Barcelona, Catalonia, Spain. Electronic address: jaumebastida@ub.edu.
authors.forename: Jaume
authors.initials: J
authors.lastname: Bastida
authors.affiliation: Faculdade de Farmácia e Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Instituto Nacional do Semi-Árido (INSA), Núcleo de Bioprospecção da Caatinga (NBioCaat), Campina Grande, Pernambuco, Brazil. Electronic address: alexandre.jose.macedo@gmail.com.
authors.forename: Alexandre José
authors.initials: AJ
authors.lastname: Macedo
authors.affiliation: Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Instituto Nacional do Semi-Árido (INSA), Núcleo de Bioprospecção da Caatinga (NBioCaat), Campina Grande, Pernambuco, Brazil. Electronic address: tiana.tasca@ufrgs.br.
authors.forename: Tiana
authors.initials: T
authors.lastname: Tasca
mesh_terms: D000994:Antitrichomonal Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001938:Brazil; D002460:Cell Line; D002470:Cell Survival; D002853:Chromatography, Liquid; D004847:Epithelial Cells / Q000187:drug effects; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006367:HeLa Cells; D006801:Humans; D032129:Manilkara / Q000737:chemistry*; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D014246:Trichomonas vaginalis / Q000187:drug effects* / Q000502:physiology; D016835:Tritrichomonas foetus / Q000187:drug effects* / Q000502:physiology
chemical_list: D000994:Antitrichomonal Agents; D005419:Flavonoids; D010936:Plant Extracts; D013634:Tannins
keywords: Manilkara rufula; Polyphenol; Sapotaceae; Tannin; Trichomonas vaginalis; Tritrichomonas foetus