title: Root-associated bacterial diversities of Oryza rufipogon and Oryza sativa and their influencing environmental factors.
abstract: Oryza rufipogon is the ancestor of human-cultivated Oryza sativa. However, little is known about the difference between the root-associated microorganisms of O. rufipogon and O. sativa. In this study, the root-associated bacteria of O. rufipogon, Leersia hexandra, and O. sativa from different latitudes in China were studied by DGGE analysis. Their bacterial community structures were compared by principal component analysis. The relationship between root-associated bacteria and soil properties was explored by canonical correspondence analysis. The relationships of glomalin-related soil protein (GRSP) content, soluble sugar content, proline content of the plant, and bacterial diversity indices of their root-associated microorganisms were also investigated. We found both broad-spectrum and host-specific bacteria, and the similarity, diversity and abundance indices of O. rufipogon and L. hexandra were higher than O. sativa root-associated bacteria. However, even living in the same habitat, O. rufipogon and L. hexandra selected different root-associated bacteria. Microbial composition was primarily correlated with available N, P, and K and the annual precipitation. We also found a positive correlation between the soluble sugar content of the plant and GRSP content of the root soil. The above results indicated that the community structure of root-associated bacteria differs between wild rice and cultivated rice. Human activity and the natural selection of the host plants shaped the differences, consistent with our hypothesis.
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Tian
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Ma
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China.|University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Shangqi
authors.initials: S
authors.lastname: Xu
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China.|School of Life Sciences, Northeast Normal University, Changchun, Jilin, China.
authors.forename: Fahad
authors.initials: F
authors.lastname: Nasir
authors.affiliation: Key Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, China. tiancj@neigae.ac.cn.
authors.forename: Chunjie
authors.initials: C
authors.lastname: Tian
mesh_terms: D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D044822:Biodiversity; D002681:China; D004777:Environment; D012275:Oryza / Q000737:chemistry / Q000382:microbiology*; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012987:Soil / Q000737:chemistry
chemical_list: D012987:Soil
keywords: DGGE; Environmental factors; Oryza rufipogon; Oryza sativa; Root-associated bacteria