title: Enhancing osteogenic differentiation of MC3T3-E1 cells by immobilizing RGD onto liquid crystal substrate.
abstract: To understand the effects of GRGDF modification on MC3T3-E1 cell behavior, we cultured these cells onto a biomimetic liquid crystalline matrix modified with GRGDF peptide (OPC-GA-RGD). Successful immobilization of GRGDF on the liquid crystalline surface was verified by fluorescent labeling, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy and X-ray photoelectron spectroscopy (XPS). OPC-GA-RGDs retained its liquid crystalline feature after surface modification. The RGD-immobilized OPC substrate was hardly beneficial to initial cell adhesion but could support long-term cell survival. The enhancement in cell proliferation did not correlate with RGD density. The lower GRGDF density immobilized on the liquid crystalline OPC matrix (OPC-GA-RGD3) promoted cell adhesion, proliferation, ALP expression level and mineralization, suggesting that both the viscoelasticity-based mechanical stimuli and receptor/ligand-based biochemical cue synergistically modulate MC3T3-E1 cell behavior.
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Shaopeng
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Wenqiang
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Du
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zeng
authors.affiliation: Department of Materials Science and Engineering, Jinan University, Guangzhou 510632, PR China; Engineering Research Center of Artificial Organs and Materials, Ministry of Education, Jinan University, Guangzhou 510632, PR China. Electronic address: tumei@jnu.edu.cn.
authors.forename: Mei
authors.initials: M
authors.lastname: Tu
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D050866:Liquid Crystals / Q000737:chemistry*; D051379:Mice; D009842:Oligopeptides / Q000737:chemistry / Q000494:pharmacology; D010012:Osteogenesis / Q000187:drug effects*
chemical_list: D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid
keywords: Cell behavior; GRGDF modification; Liquid crystal; Osteogenic differentiation