title: Analyses of HBV cccDNA Quantification and Modification.
abstract: Covalently closed circular DNA (cccDNA) serves as the transcriptional template of hepatitis B virus (HBV) replication in the nucleus of infected cells. It ensures the persistence of HBV even if replication is blocked. Immune-mediated killing of infected hepatocytes, cell division, or cytokine induced non-cytolytic degradation of cccDNA can induce the loss of cccDNA. For studies on HBV control, the analysis of cccDNA integrity and its exact quantification is very important. Here, we describe different methods for HBV cccDNA quantification and modification.
authors.affiliation: Institute of Virology, Technische Universität München/Helmholtz Zentrum, Munich, Germany.
authors.forename: Yuchen
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Institute of Virology, Technische Universität München/Helmholtz Zentrum, Munich, Germany.
authors.forename: Daniela
authors.initials: D
authors.lastname: Stadler
authors.affiliation: Institute of Virology, Technische Universität München/Helmholtz Zentrum, Munich, Germany.
authors.forename: Chunkyu
authors.initials: C
authors.lastname: Ko
authors.affiliation: Institute of Virology, Technische Universität München/Helmholtz Zentrum, Munich, Germany. protzer@tum.de.
authors.forename: Ulrike
authors.initials: U
authors.lastname: Protzer
mesh_terms: D004270:DNA, Circular; D004279:DNA, Viral; D006509:Hepatitis B / Q000821:virology*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D060888:Real-Time Polymerase Chain Reaction; D019562:Viral Load
chemical_list: D004270:DNA, Circular; D004279:DNA, Viral
keywords: Covalently closed circular DNA; Deamination; Differential DNA denaturation PCR; Quantitative PCR; Quantitative differential DNA denaturation PCR