title: Multiresidue Screening of Veterinary Drugs in Meat, Milk, Egg, and Fish Using Liquid Chromatography Coupled with Ion Trap Time-of-Flight Mass Spectrometry.
abstract: New approaches to veterinary drug screening based on liquid chromatography-mass spectrometry (LC-MS/MS) and time-of-flight mass spectrometry (ToF/MS) are rapid and have high selectivity and sensitivity. In this study, we developed a multiresidue method for screening over 100 veterinary drug residues using ion trap (IT)-ToF/MS. The screened compounds comprised major drug classes used in veterinary practice, representing the following: amphenicols, anthelmintics, benzimidazoles, β-lactams, coccidiostats, ionophores, macrolides, non-steroidal anti-inflammatory drugs, quinolones, sulfonamides, tetracyclines, and tranquilizers. The method was developed based on chromatographic retention time, specific accurate mass, isotope distribution, and fragment data. Each compound was validated at three levels, and the mass accuracy, accuracy, and repeatability were calculated. All parameters showed acceptable values and conformed to the Commission Decision 2002/657/EC criteria. This screening method can simultaneously analyze over 100 veterinary drugs in meat, milk, eggs, and fish in a single analytical run.
authors.affiliation: Veterinary Drugs and Biologics Division, Animal and Plant Quarantine and Inspection Agency, Gyeonggi-do, 430-757, Republic of Korea.
authors.forename: JeongWoo
authors.initials: J
authors.lastname: Kang
authors.affiliation: Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Chungcheongbuk-do, 363-700, Republic of Korea.
authors.forename: Su-Jeong
authors.initials: SJ
authors.lastname: Park
authors.affiliation: Veterinary Drugs and Biologics Division, Animal and Plant Quarantine and Inspection Agency, Gyeonggi-do, 430-757, Republic of Korea.
authors.forename: Hae-Chul
authors.initials: HC
authors.lastname: Park
authors.affiliation: Veterinary Drugs and Biologics Division, Animal and Plant Quarantine and Inspection Agency, Gyeonggi-do, 430-757, Republic of Korea.
authors.forename: Md Akil
authors.initials: MA
authors.lastname: Hossain
authors.affiliation: Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Chungcheongbuk-do, 363-700, Republic of Korea.
authors.forename: Myeong-Ae
authors.initials: MA
authors.lastname: Kim
authors.affiliation: Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Chungcheongbuk-do, 363-700, Republic of Korea.
authors.forename: Seong-Wan
authors.initials: SW
authors.lastname: Son
authors.affiliation: Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Chungcheongbuk-do, 363-700, Republic of Korea.
authors.forename: Chae-Mi
authors.initials: CM
authors.lastname: Lim
authors.affiliation: Department of Physiology, College of Veterinary Medicine, Kyungpook National University, Daegu, 702-701, Republic of Korea. twkim@knu.ac.kr.
authors.forename: Tae-Wan
authors.initials: TW
authors.lastname: Kim
authors.affiliation: Ministry of Food and Drug Safety, Osong Health Technology Administration Complex, Chungcheongbuk-do, 363-700, Republic of Korea. chobh02@korea.kr.
authors.forename: Byung-Hoon
authors.initials: BH
authors.lastname: Cho
mesh_terms: D002853:Chromatography, Liquid; D005396:Fish Products / Q000032:analysis*; D005504:Food Analysis / Q000379:methods*; D013058:Mass Spectrometry; D008460:Meat / Q000032:analysis*; D010063:Ovum; D019155:Veterinary Drugs / Q000032:analysis*
chemical_list: D019155:Veterinary Drugs
keywords: Detection; LC-IT-ToF/MS; Multiresidue; Screening; Veterinary drugs