title: Co-expression changes of lncRNAs and mRNAs in the cervical sympathetic ganglia in diabetic cardiac autonomic neuropathic rats.
abstract: Cardiac autonomic neuropathy in Type 2 diabetes (T2D) is often a devastating complication. Long non-coding RNAs (lncRNAs) have important effects on both normal development and disease pathogenesis. In this study, we explored the expression profiles of some lncRNAs involved in inflammation which may be co-expressed with messenger RNA (mRNA) in superior cervical and stellate ganglia after type 2 diabetic injuries. Total RNA isolated from 10 pairs of superior cervical and stellate ganglia in diabetic and normal male rats was hybridized to lncRNA arrays for detections. Pathway analysis indicated that the most significant gene ontology (GO) processes that were upregulated in diabetes were associated with immune response, cell migration, defense response, taxis, and chemotaxis. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway revealed that most of the target genes of the lncRNAs were located in cytokine-cytokine receptor interactions, the chemokine signaling pathway and cell adhesion molecules, which were involved in T2D. Gene co-expression network construction showed that the co-expression network in the experimental rats consisted of 268 regulation edges among 105 lncRNAs and 11 mRNAs. Our studies demonstrated the co-expression profile of lncRNAs and mRNAs in diabetic cardiac autonomic ganglia, suggesting possible roles for multiple lncRNAs as potential targets for the development of therapeutic strategies or biomarkers for diabetic cardiac autonomic neuropathy. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Sheng
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Yurong
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Huaide
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Chaoran
authors.initials: C
authors.lastname: Zheng
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Guo
authors.affiliation: Undergraduate Student of Second Clinical Medical College, Medical College of Nanchang University, Nanchang, 330008, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Sun
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Endocrine Department of Third Affiliated Hospital, Medical College of Nanchang University, Nanchang, 330008, PR China.
authors.forename: Shulan
authors.initials: S
authors.lastname: Qin
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Medical Genetics and Biology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
authors.affiliation: Department of Anatomy, Basic Medical College of Nanchang University, Nanchang, 330006, PR China.
authors.forename: Gaochun
authors.initials: G
authors.lastname: Zhu
mesh_terms: D000818:Animals; D001794:Blood Pressure / Q000502:physiology; D002784:Cholesterol / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental; D003929:Diabetic Neuropathies / Q000378:metabolism* / Q000473:pathology*; D004195:Disease Models, Animal; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006339:Heart Rate / Q000502:physiology; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D017783:Superior Cervical Ganglion / Q000378:metabolism* / Q000473:pathology
chemical_list: D062085:RNA, Long Noncoding; D012333:RNA, Messenger; D002784:Cholesterol
keywords: Long non-coding RNA; RRID: RGD_734476; RRID: SCR_004899; diabetic cardiac autonomic neuropathy; stellate ganglia; superior cervical ganglia