title: Deinococcus rubrussp. nov., a Bacterium Isolated from Antarctic Coastal Sea Water.
abstract: Two Gram-staining-negative, red-pinkish, coccus-shaped, non-motile, and aerobic bacterial strains, designated Ant21Tand Ant22, were isolated from the Antarctic coastal sea water. Strains Ant21Tand Ant22 showed UVC and gamma radiation resistance. Phylogenetic analyses based on 16SrRNA gene sequences determined that these strains belong to the genusDeinococcus. Through the analyses of the 16SrRNA gene sequences, strains Ant21Tand Ant22 were found to have 97.7% and 97.8% similarity toDeinococcus marmorisDSM 12784Tand 97.0% and 97.2% similarity toDeinococcus saxicolaAA-1444T, respectively. The sequence similarity with the type strains of otherDeinococcusspecies was less than 96.9% for both strains. Strains Ant21Tand Ant22 shared relatively high 16SrRNA gene sequence similarity (99.3%) and had a closely related DNA reassociation value of 84 ± 0.5%. Meanwhile, they showed a low level of DNA-DNA hybridization (<30%) with other closely related species of the genusDeinococcus. The two strains also showed typical chemotaxonomic features for the genusDeinococcus, in terms of the major polar lipid (phosphoglycolipid) and the major fatty acids (C16:0, C16:1ω6c/ω7c, iso-C17:0, and iso-C15:0). They grew at temperatures between 4°C and 30°C and at pH values of 6.0-8.0. Based on the physiological characteristics, the 16S rRNA gene sequence analysis results, and the low DNA-DNA reassociation level withDeionococcus marmoris, strains Ant21T(= KEMB 9004-167T=JCM 31436T) and Ant22 (KEMB 9004-168 =JCM 31437) represent novel species belonging to the genusDeinococcus, for which the nameDeinococcus rubrusis proposed.
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 01797, Korea.
authors.forename: Sathiyaraj
authors.initials: S
authors.lastname: Srinivasan
authors.affiliation: Radiation Research Division for Biotechnology, Korea Atomic Energy Research Institute, Jeongeup 56212, Republic of Korea.
authors.forename: Sangyong
authors.initials: S
authors.lastname: Lim
authors.affiliation: Marine Environment Research Division, National Institute of Fisheries Science, Busan 46083, Republic of Korea.
authors.forename: Jae-Hyun
authors.initials: JH
authors.lastname: Lim
authors.affiliation: College of Agricultural and Life Sciences, Kyungpook National University, Daegu 41566, Republic of Korea.|Institute of Plant Medicine, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
authors.affiliation: Department of Bio & Environmental Technology, College of Natural Science, Seoul Women's University, Seoul 01797, Korea.
authors.forename: Myung Kyum
authors.initials: MK
authors.lastname: Kim
mesh_terms: D000864:Antarctic Regions; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D058893:DNA Barcoding, Taxonomic; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005798:Genes, Bacterial; D016680:Genome, Bacterial; D010641:Phenotype; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D014871:Water Microbiology
chemical_list: D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Deinococcus; coastal sea water; radiation resistance; taxonomy