title: Human polyomavirus DNA detection in keratoacanthoma and Spitz naevus: no evidence for a causal role.
abstract: Keratoacanthomas (KA) and Spitz naevus (SN) are both lesions with unknown aetiology; therefore, the possibility of a viral involvement, more specifically the involvement of human polyomaviruses (HPyV), was investigated. In total, 22 cases of KA and 25 cases of SN were tested for the presence of HPyVs. DNA was extracted and amplified by multiplex PCR and thereafter tested with a multiplex bead-based assay for HPyVs (BKPyV, JCPyV, KIPyV, WUPyV, MCPyV, TSPyV, HPyV6, 7 and 9) and two primate viruses (SV40 and LPyV). HPyV DNA was found in 20 of the 47 lesions. There was no significant difference in HPyV DNA detection frequency between patients diagnosed with KA and patients diagnosed with SN, nor any over-representation of a specific HPyV type in any of the two patient categories. In conclusion, evidence for a specific aetiological role of any of the above tested HPyVs in either KA or SN was not disclosed.
authors.affiliation: Department of oncology and pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Linnea
authors.initials: L
authors.lastname: Haeggblom
authors.affiliation: Department of oncology and pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Joar
authors.initials: J
authors.lastname: Franzén
authors.affiliation: Department of oncology and pathology, Karolinska Institutet, Stockholm, Sweden.
authors.forename: Anders
authors.identifier: http://orcid.org/0000-0003-4602-4297
authors.initials: A
authors.lastname: Näsman
mesh_terms: D000328:Adult; D000368:Aged; D004279:DNA, Viral / Q000032:analysis / Q000235:genetics; D006801:Humans; D007636:Keratoacanthoma / Q000175:diagnosis / Q000821:virology*; D008875:Middle Aged; D060885:Multiplex Polymerase Chain Reaction; D018332:Nevus, Epithelioid and Spindle Cell / Q000175:diagnosis / Q000821:virology*; D016612:Paraffin Embedding; D011120:Polyomavirus / Q000235:genetics / Q000302:isolation & purification*; D027601:Polyomavirus Infections / Q000175:diagnosis / Q000821:virology*; D012878:Skin Neoplasms / Q000175:diagnosis / Q000821:virology*; D014412:Tumor Virus Infections / Q000175:diagnosis / Q000821:virology*; D055815:Young Adult
chemical_list: D004279:DNA, Viral
keywords: PAPILLOMA VIRUSES; SKIN; SKIN TUMOURS