title: Characterization of a novel disease resistance gene rtp3 and its association with VNN disease resistance in Asian seabass.
abstract: Asian seabass, an important food fish in Southeast Asia, has suffered from nervous necrosis virus (NNV) infection, resulting in massive mortality of Asian seabass larvae and enormous economic losses. Identification and characterization of disease resistance genes is important. Previous transcriptome analysis of Asians seabass epithelial cells after NNV infection revealed a highly inducible gene, receptor-transporting protein 3 (rtp3), indicating it could play an important role in Asian seabass - NNV interaction. To characterize this gene, we determined its expression pattern and subcellular localization. The rtp3 was highly induced in most examined tissues and organs of Asian seabass after NNV infection, and protein Rtp3 was localized in cytoplasm. Further association study in multiple families revealed that a microsatellite marker, (GT)ntt(GT)n, in the 3' UTR of rtp3 was significantly associated with VNN disease resistance in Asian seabass. Our results imply that rtp3 may be a novel disease resistance gene in Asian seabass. This data could improve our understanding of molecular interaction between Asian seabass and NNV, and has the potential to be applied in marker-assisted selection for disease resistance breeding in Asian seabass.
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive, 117543 Singapore; Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604 Singapore.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604 Singapore.
authors.forename: Le
authors.initials: L
authors.lastname: Wang
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604 Singapore.
authors.forename: Bao Qing
authors.initials: BQ
authors.lastname: Ye
authors.affiliation: Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604 Singapore.
authors.forename: Shuqing
authors.initials: S
authors.lastname: Huang
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive, 117543 Singapore; Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604 Singapore; National University of Singapore Suzhou Research Institute, Suzhou, Jiangsu, 215123 China. Electronic address: dbswsm@nus.edu.sg.
authors.forename: Sek-Man
authors.initials: SM
authors.lastname: Wong
authors.affiliation: Department of Biological Sciences, National University of Singapore, 14 Science Drive, 117543 Singapore; Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, 117604 Singapore; School of Biological Sciences, Nanyang Technological University, 6 Nanyang Drive, 637551 Singapore. Electronic address: genhua@tll.org.sg.
authors.forename: Gen Hua
authors.initials: GH
authors.lastname: Yue
mesh_terms: D000818:Animals; D060467:Disease Resistance; D005393:Fish Diseases / Q000235:genetics* / Q000276:immunology / Q000821:virology; D015870:Gene Expression; D020869:Gene Expression Profiling / Q000662:veterinary; D018895:Microsatellite Repeats; D030381:Nodaviridae / Q000502:physiology; D010473:Perciformes; D012327:RNA Virus Infections / Q000235:genetics / Q000276:immunology / Q000662:veterinary* / Q000821:virology
keywords: Asian seabass; Breeding; NNV; VNN disease; rtp3