title: EIN3 interferes with the sulfur deficiency signaling in Arabidopsis thaliana through direct interaction with the SLIM1 transcription factor.
abstract: Sulfur deficiency in plants leads to metabolic reprogramming through changes of gene expression. SLIM1 is so far the only characterized transcription factor associated strictly with sulfur deficiency stress in Arabidopsis thaliana. It belongs to the same protein family as EIN3, a major positive switch of ethylene signaling pathway. It binds to the specific cis sequence called UPE-box. Here we show that SLIM1 interacts with UPE-box as a homodimer. Interestingly, the same region of the protein is used for heterodimerization with EIN3; however, the heterodimer is not able to recognize UPE-box. Expression of several SLIM1-dependent genes is enhanced in sulfur deficiency grown Arabidopsis ein3-1 seedlings (with mutated EIN3 protein). This implies a possible regulatory mechanism of ethylene in sulfur metabolism through direct EIN3-SLIM1 interaction.
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A St., 02-106 Warsaw, Poland. Electronic address: blaszczyk@ibb.waw.pl.
authors.forename: Anna
authors.initials: A
authors.lastname: Wawrzyńska
authors.affiliation: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawińskiego 5A St., 02-106 Warsaw, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sirko
mesh_terms: D017360:Arabidopsis / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism*; D009687:Nuclear Proteins / Q000378:metabolism*; D013455:Sulfur / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D004268:DNA-Binding Proteins; C500268:EIL3 protein, Arabidopsis; C106987:EIN3 protein, Arabidopsis; D009687:Nuclear Proteins; D014157:Transcription Factors; D013455:Sulfur
keywords: Arabidopsis; EIN3; Ethylene signaling; SLIM1; Sulfur deficiency; Transcriptional regulation