title: Pseudomonas aeruginosaAirway Infection Recruits and Modulates Neutrophilic Myeloid-Derived Suppressor Cells.
abstract: Pseudomonas aeruginosais an opportunistic pathogen that causes infections mainly in patients with cystic fibrosis (CF) lung disease. Despite innate and adaptive immune responses upon infection,P. aeruginosais capable of efficiently escaping host defenses, but the underlying immune mechanisms remain poorly understood. Myeloid-derived suppressor cells (MDSCs) are innate immune cells that are functionally characterized by their potential to suppress T- and natural killer (NK)-cell responses. Here we demonstrate, using an airwayin vivoinfection model, thatP. aeruginosarecruits and activates neutrophilic MDSCs, which functionally suppress T-cell responses. We further show that the CF gene defect (CF transmembrane conductance regulator, CFTR) modulates the functionality, but not the recruitment or generation of neutrophilic MDSCs. Collectively, we define a mechanism by whichP. aeruginosaairway infection undermines host immunity by modulating neutrophilic MDSCsin vivo.
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tübingen Tübingen, Germany.
authors.forename: Hasan H
authors.initials: HH
authors.lastname: Öz
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tübingen Tübingen, Germany.
authors.forename: Benyuan
authors.initials: B
authors.lastname: Zhou
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tübingen Tübingen, Germany.
authors.forename: Pina
authors.initials: P
authors.lastname: Voss
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tübingen Tübingen, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Carevic
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tübingen Tübingen, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Schroth
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tübingen Tübingen, Germany.
authors.forename: Nina
authors.initials: N
authors.lastname: Frey
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of TübingenTübingen, Germany; Department of Pediatrics, Kinderklinik München Schwabing, Klinikum Schwabing, StKM GmbH und Klinikum rechts der Isar, Technische Universität MünchenMunich, Germany.
authors.forename: Nikolaus
authors.initials: N
authors.lastname: Rieber
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of Tübingen Tübingen, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Hector
authors.affiliation: Children's Hospital and Interdisciplinary Center for Infectious Diseases, University of TübingenTübingen, Germany; Roche Pharma Research and Early Development, Immunology, Inflammation and Infectious Diseases (I3) Discovery and Translational Area, Roche Innovation Center BaselBasel, Switzerland.
authors.forename: Dominik
authors.initials: D
authors.lastname: Hartl
mesh_terms: D000818:Animals; D019005:Cystic Fibrosis Transmembrane Conductance Regulator / Q000378:metabolism; D057131:Immune Evasion; D007108:Immune Tolerance; D008810:Mice, Inbred C57BL; D018181:Mice, Inbred CFTR; D000072737:Myeloid-Derived Suppressor Cells / Q000276:immunology*; D009504:Neutrophils / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D012141:Respiratory Tract Infections / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology
chemical_list: D019005:Cystic Fibrosis Transmembrane Conductance Regulator
keywords: CFTR; MDSCs; Pseudomonas; T-cells; bacteria; cystic fibrosis; lung