title: The human intestinal microbiota of constipated-predominant irritable bowel syndrome patients exhibits anti-inflammatory properties.
abstract: The intestinal microbiota of patients with constipated-predominant irritable bowel syndrome (C-IBS) displays chronic dysbiosis. Our aim was to determine whether this microbial imbalance instigates perturbation of the host intestinal mucosal immune response, using a model of human microbiota-associated rats (HMAR) and dextran sulfate sodium (DSS)-induced experimental colitis. The analysis of the microbiota composition revealed a decrease of the relative abundance of Bacteroides, Roseburia-Eubacterium rectale and Bifidobacterium and an increase of Enterobacteriaceae, Desulfovibrio sp., and mainly Akkermansia muciniphila in C-IBS patients compared to healthy individuals. The bacterial diversity of the gut microbiota of healthy individuals or C-IBS patients was maintained in corresponding HMAR. Animals harboring a C-IBS microbiota had reduced DSS colitis with a decreased expression of pro-inflammatory cytokines from innate, Th1, and Th17 responses. The pre-treatment of conventional C57BL/6 mice or HMAR with A. muciniphila, but not with Escherichia coli, prior exposure to DSS also resulted in a reduction of colitis severity, highlighting that the anti-inflammatory effect of the gut microbiota of C-IBS patients is mediated, in part, by A. muciniphila. This work highlights a novel aspect of the crosstalk between the gut microbiota of C-IBS patients and host intestinal homeostasis.
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR454 Microbiologie, Saint-Genès-Champanelle, France.|Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.|Center for Mucosal Inflammation and Cancer, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
authors.forename: Alain P
authors.initials: AP
authors.lastname: Gobert
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR454 Microbiologie, Saint-Genès-Champanelle, France.
authors.forename: Giulia
authors.initials: G
authors.lastname: Sagrestani
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR454 Microbiologie, Saint-Genès-Champanelle, France.
authors.forename: Eve
authors.initials: E
authors.lastname: Delmas
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.|Center for Mucosal Inflammation and Cancer, Vanderbilt University Medical Center, Nashville, Tennessee, USA.|Department of Pathology, Microbiology, and Immunology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.|Department of Cancer Biology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.|Veterans Affairs Tennessee Valley Healthcare System, Nashville, Tennessee, USA.
authors.forename: Keith T
authors.initials: KT
authors.lastname: Wilson
authors.affiliation: Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
authors.forename: Thomas G
authors.initials: TG
authors.lastname: Verriere
authors.affiliation: CHU Clermont-Ferrand, Service de Médecine Digestive et Hépatobiliaire, Hôpital Estaing, Clermont-Ferrand, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Dapoigny
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR454 Microbiologie, Saint-Genès-Champanelle, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Del'homme
authors.affiliation: Institut National de la Recherche Agronomique (INRA), UR454 Microbiologie, Saint-Genès-Champanelle, France.
authors.forename: Annick
authors.initials: A
authors.lastname: Bernalier-Donadille
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000276:immunology*; D003092:Colitis / Q000276:immunology / Q000382:microbiology; D016207:Cytokines / Q000276:immunology; D016264:Dextran Sulfate / Q000276:immunology; D064806:Dysbiosis / Q000276:immunology; D000069196:Gastrointestinal Microbiome / Q000276:immunology*; D006801:Humans; D007249:Inflammation / Q000276:immunology; D007422:Intestines / Q000382:microbiology*; D043183:Irritable Bowel Syndrome / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL; D064307:Microbiota / Q000276:immunology; D051381:Rats
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D016264:Dextran Sulfate