title: Magnetic Fe3O4nanoparticles induced effects on performance and microbial community of activated sludge from a sequencing batch reactor under long-term exposure.
abstract: The performance and microbial community of activated sludge from a sequencing batch reactor (SBR) were investigated under long-term exposure of magnetic Fe3O4nanoparticles (Fe3O4NPs). The COD removal showed a slight decrease at 5-60mg/L Fe3O4NPs compared to 0mg/L Fe3O4NPs, whereas the NH4+-N removal had no obvious variation at 0-60mg/L Fe3O4NPs. It was found that 10-60mg/L Fe3O4NPs improved the denitrification process and phosphorus removal of activated sludge. The microbial enzymatic activities of activated sludge could be affected by Fe3O4NPs, which had similar variation trends to the nitrogen and phosphorus removal rates of activated sludge. The reactive oxygen species (ROS) production and lactate dehydrogenase (LDH) release demonstrated that Fe3O4NPs led to the toxicity to activated sludge and destroyed the integrity of microbial cytomembrane. High throughput sequencing indicated that Fe3O4NPs could obviously affect the microbial richness and diversity of activated sludge.
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Bingrui
authors.initials: B
authors.lastname: Ma
authors.affiliation: Shcool of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, China. Electronic address: mengchungao@outlook.com.
authors.forename: Mengchun
authors.initials: M
authors.lastname: Gao
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China; Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering, Ocean University of China, Qingdao 266100, China.
authors.forename: Zonglian
authors.initials: Z
authors.lastname: She
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Yangguo
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zheng
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Chunji
authors.initials: C
authors.lastname: Jin
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Xuejiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification; D052203:Ferrosoferric Oxide / Q000737:chemistry; D007770:L-Lactate Dehydrogenase / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D059013:Microbial Consortia / Q000187:drug effects / Q000502:physiology*; D009584:Nitrogen / Q000032:analysis / Q000378:metabolism; D010758:Phosphorus / Q000302:isolation & purification / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012722:Sewage / Q000382:microbiology*; D014865:Waste Disposal, Fluid / Q000295:instrumentation* / Q000379:methods
chemical_list: D058185:Magnetite Nanoparticles; D017382:Reactive Oxygen Species; D012722:Sewage; D010758:Phosphorus; D007770:L-Lactate Dehydrogenase; D009584:Nitrogen; D052203:Ferrosoferric Oxide
keywords: Magnetic Fe(3)O(4) NPs; Microbial enzymatic activity; Microbial richness and diversity; Nitrogen and phosphorus removal; Reactive oxygen species