title: Psychrophilic Lipase from Arctic Bacterium.
abstract: A lipase producer psychrophilic microorganism isolated from Arctic sample was studied. The genomic DNA of the isolate was extracted using modified CTAB method. Identification of the isolate by morphological and 16S rRNA sequence analysis revealed that the isolate is closely related toArthrobacter gangotriensis(97% similarity).A. gangotriensiswas determined as positive lipase producer based on the plate screening using specific and sensitive plate assay of Rhodamine B. The PCR result usingArthrobactersp.'s full lipase gene sequence as the template primers emphasised a possible lipase gene at 900 bp band size. The gene is further cloned in a suitable vector system for expression of lipase.
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Zakiah
authors.initials: Z
authors.lastname: Ramle
authors.affiliation: School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia.
authors.forename: Rashidah Abdul
authors.initials: RA
authors.lastname: Rahim
keywords: Arctic; Lipase; Psychrophiles