title: Planococcus versutus sp. nov., isolated from soil.
abstract: A taxonomic study was performed on a novel Gram-stain-positive, coccus-shaped, orange-pigmented motile bacterium, designated as strain L10.15T. The organism was isolated from a soil sample collected in Lagoon Island (close to Adelaide Island, western Antarctic Peninsula) using a quorum-quenching enrichment medium. Growth occurred at 4-30 °C, pH 6-11 and at moderately high salinity (0-15 %, w/v, NaCl), with optimal growth at 26 °C, at pH 7-8 and with 6 % (w/v) NaCl. 16S rRNA gene sequence analysis showed that strain L10.15Tbelonged to the genus Planococcus and was closely related to Planococcus halocryophilus Or1T(99.3 % similarity), Planococcus donghaensis JH1T(99.0 %), Planococcus antarcticus DSM 14505T(98.3 %), Planococcus plakortidis AS/ASP6 (II)T(97.6 %), Planococcus maritimus TF-9T(97.5 %), Planococcus salinarum ISL-6T(97.5 %) and Planococcus kocurii NCIMB 629T(97.5 %). However, the average nucleotide identity-MUMmer analysis showed low genomic relatedness values of 71.1-81.7 % to the type strains of these closely related species of the genus Planococcus. The principal fatty acids were anteiso-C15 : 0, C16 : 1ω7c and anteiso-C17 :  0, and the major menaquinones of strain L10.15Twere MK-5 (48 %), MK-6 (6 %) and MK-7 (44 %). Polar lipid analysis revealed the presence of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophospholipid. The DNA G+C content was 39.4 mol%. The phenotypic and genotypic data indicate that strain L10.15Trepresents a novel species of the genus Planococcus, for which the name Planococcus versutus sp. nov. is proposed. The type strain is L10.15T(=DSM 101994T=KACC 18918T).
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.|National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Wah-Seng
authors.initials: WS
authors.lastname: See-Too
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Robson
authors.initials: R
authors.lastname: Ee
authors.affiliation: Temasek Lifesciences Laboratory, National University of Singapore, 1 Research Link 117604, Singapore.
authors.forename: Munusamy
authors.initials: M
authors.lastname: Madhaiyan
authors.affiliation: National Agrobiodiversity Center, National Academy of Agricultural Science, Suwon 441-707, Republic of Korea.
authors.forename: Soon-Wo
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Jia Yi
authors.initials: JY
authors.lastname: Tan
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Yan Lue
authors.initials: YL
authors.lastname: Lim
authors.affiliation: National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.|British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, UK.
authors.forename: Peter
authors.initials: P
authors.lastname: Convey
authors.affiliation: National Antarctic Research Centre (NARC), Institute of Postgraduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia.|British Antarctic Survey, NERC, High Cross, Madingley Road, Cambridge CB3 OET, UK.|Faculty of Health and Life Sciences, University of Northumbria, Newcastle Upon Tyne NE1 8ST, UK.
authors.forename: David A
authors.initials: DA
authors.lastname: Pearce
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Wai Fong
authors.initials: WF
authors.lastname: Yin
authors.affiliation: Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.|UM Omics Centre, University of Malaya, Kuala Lumpur, Malaysia.
authors.forename: Kok-Gan
authors.initials: KG
authors.lastname: Chan
mesh_terms: D000864:Antarctic Regions; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids; D010802:Phylogeny; D058642:Planococcus Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014451:Ubiquinone / Q000737:chemistry; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; D014451:Ubiquinone; C042455:menaquinone 6; C062629:menaquinone 7; C025203:Ubiquinone Q1
keywords: Antarctic soil; Planococcus versutus; quorum quenching