title: CenpH regulates meiotic G2/M transition by modulating the APC/CCdh1-cyclin B1 pathway in oocytes.
abstract: Meiotic resumption (G2/M transition) and progression through meiosis I (MI) are two key stages for producing fertilization-competent eggs. Here, we report that CenpH, a component of the kinetochore inner plate, is responsible for G2/M transition in meiotic mouse oocytes. Depletion of CenpH by morpholino injection decreased cyclin B1 levels, resulting in attenuation of maturation-promoting factor (MPF) activation, and severely compromised meiotic resumption. CenpH protects cyclin B1 from destruction by competing with the action of APC/CCdh1Impaired G2/M transition after CenpH depletion could be rescued by expression of exogenous cyclin B1. Unexpectedly, blocking CenpH did not affect spindle organization and meiotic cell cycle progression after germinal vesicle breakdown. Our findings reveal a novel role of CenpH in regulating meiotic G2/M transition by acting via the APC/CCdh1-cyclin B1 pathway.
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.|Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.|University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.|Department of Reproductive Medicine, Guangdong Women and Children Hospital, Guangzhou 510010, China.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Wang
authors.affiliation: Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Shen
authors.affiliation: Department of Veterinary Pathobiology, University of Missouri, Columbia, MO 65211, USA.
authors.forename: Heide
authors.initials: H
authors.lastname: Schatten
authors.affiliation: State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China sunqy@ioz.ac.cn.|Institute of Reproductive Sciences, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.|University of the Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Qing-Yuan
authors.identifier: 0000-0002-0148-2414
authors.initials: QY
authors.lastname: Sun
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D002455:Cell Division / Q000235:genetics; D002868:Chromosomal Proteins, Non-Histone / Q000502:physiology*; D056744:Cyclin B1 / Q000235:genetics* / Q000378:metabolism; D005260:Female; D016195:G2 Phase / Q000235:genetics; D059565:G2 Phase Cell Cycle Checkpoints / Q000235:genetics; D005786:Gene Expression Regulation; D008540:Meiosis / Q000235:genetics*; D000090204:Mesothelin; D051379:Mice; D008813:Mice, Inbred ICR; D008822:Mice, Transgenic; D009865:Oocytes / Q000378:metabolism*; D015398:Signal Transduction / Q000235:genetics
chemical_list: C000622817:CenpH protein, mouse; D002868:Chromosomal Proteins, Non-Histone; D056744:Cyclin B1; C000715528:Msln protein, mouse; D064173:Anaphase-Promoting Complex-Cyclosome; D000090204:Mesothelin
keywords: APC/CCdh1; CenpH; Cyclin B1; Fzr1; G2/M transition; Meiosis; Oocyte