title: Haoranjiania flava gen. nov., sp. nov., a new member of the family Chitinophagaceae, isolated from activated sludge.
abstract: A novel Gram-stain-negative, non-spore-forming and short rod-shaped bacterium, strain LIP-5T, isolated from an activated sludge in a pesticide factory in Xinyi, China, was investigated for its taxonomic position by a polyphasic approach. Cell growth occurred at 16-42 °C (optimum, 30 °C), in the presence of 0-3.0 % (w/v) NaCl (optimum, without NaCl) and at pH 6.0-9.0 (optimum, pH 7.0). Phylogenetic analysis based on 16S rRNA gene sequence comparisons showed that the isolate was a member of the family Chitinophagaceae, with the closest relatives being Arachidicoccus rhizosphaerae Vu-144T (93.5 % similarity), followed by Heliimonas saccharivorans L2-4T (90.5 %) and Chitinophaga ginsengisoli Gsoil 052T (89.6 %). Menaquinone 7 (MK-7) was the predominant respiratory ubiquinone and phosphatidylethanolamine and unidentified lipids were the major polar lipids. The major cellular fatty acids of strain LIP-5T were anteiso-C15 : 0 and iso-C15 : 0, and there were also moderate amounts of iso-C17 : 0 3-OH and C17 : 0 2-OH. The DNA G+C content was 42.3 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain LIP-5T was classified as representing a novel species of a new genus, for which the name Haoranjiania flava gen. nov., sp. nov. is proposed. The type strain of Haoranjiania flava is LIP-5T (=CCTCC AB 2015365T=KCTC 42956T).
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yan
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Shun-Peng
authors.initials: SP
authors.lastname: Li
authors.affiliation: Institute of Plant Sciences, Newe-Ya'ar Research Center, Agricultural Research Organization, PO Box 1021, Ramat Yishay 30095, Israel.
authors.forename: Shiri
authors.initials: S
authors.lastname: Freilich
authors.affiliation: Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
authors.forename: Jian-Dong
authors.initials: JD
authors.lastname: Jiang
mesh_terms: D015373:Bacterial Typing Techniques; D041963:Bacteroidetes / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010714:Phosphatidylethanolamines; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D024482:Vitamin K 2; C483858:phosphatidylethanolamine; C062629:menaquinone 7