title: Hydrogel Functionalized Janus Membrane for Skin Regeneration.
abstract: In this study, a hydrogel functionalized Janus membrane is developed and its capacity is examined as a wound dressing biomaterial. A hydrophobic fluoropolymer, poly(3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,10-heptadecafluorodecyl methacrylate) (PHFDMA), is uniformly coated onto macroporous polyester membrane through initiated chemical vapor deposition process on both sides. PHFDMA-coated macroporous membrane exhibits antibacterial property, allows air permeation, and inhibits water penetration. Janus membrane property is obtained by exposing one side of PHFDMA coated membrane with 1 m KOH solution, which allows PHFDMA cleavage resulting in carboxylic acid residue. This carboxylic acid residue is then further functionalized with gelatin methacrylate-based photocrosslinkable hydrogel for moisture retention and growth factor release. When applied to full thickness dorsal skin defect model, functionalized hydrogel allows moisture retention and hydrophobic surface prevents exudate leaks via water repellence. Furthermore, hydrogel functionalized Janus membrane enhances the wound healing rate and induces thick epidermal layer formation. In conclusion, the multifunctional Janus membrane with hydrophobic outer surface and immobilized hydrogel on the other surface is fabricated for an innovative strategy for wound healing.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, 152-742, Republic of Korea.
authors.forename: Young-Hyeon
authors.initials: YH
authors.lastname: An
authors.affiliation: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea.
authors.forename: Seung Jung
authors.initials: SJ
authors.lastname: Yu
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, 152-742, Republic of Korea.
authors.forename: In Seon
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Su-Hwan
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Jeong-Mi
authors.initials: JM
authors.lastname: Moon
authors.affiliation: Interdisciplinary Program in Bioengineering, Seoul National University, Seoul, 151-742, Republic of Korea.
authors.forename: Seunghyun L
authors.initials: SL
authors.lastname: Kim
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, 152-742, Republic of Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Choi
authors.affiliation: Department of Biochemistry, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Joon Sig
authors.initials: JS
authors.lastname: Choi
authors.affiliation: Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology, Daejeon, 305-701, Republic of Korea.
authors.forename: Sung Gap
authors.initials: SG
authors.lastname: Im
authors.affiliation: College of Pharmacy, Chungbuk National University, Cheongju, 362-763, Republic of Korea.
authors.forename: Kyung Eun
authors.initials: KE
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul, 152-742, Republic of Korea.
authors.forename: Nathaniel S
authors.initials: NS
authors.lastname: Hwang
mesh_terms: D000818:Animals; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D008567:Membranes, Artificial; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D041681:NIH 3T3 Cells; D012867:Skin / Q000293:injuries* / Q000378:metabolism / Q000473:pathology; D014945:Wound Healing
chemical_list: D020100:Hydrogels; D008567:Membranes, Artificial
keywords: hydrogel immobilization; initiated chemical vapor deposition; protein delivery; wound dressing; wound healing