title: Comparison of Experimental Diabetic Periodontitis Induced byPorphyromonas gingivalisin Mice.
abstract: Periodontitis is one of the severe complications in diabetic patients and gingival epithelium plays an initial role on the onset and progression of this disease. However the potential mechanism is yet sufficiently understood. Meanwhile, the research on the correlational experimental animal models was also insufficient. Here, we established periodontitis with type 2 diabetes indb/dband Tallyho/JngJ (TH) mice and periodontitis with type 1 diabetes in streptozotocin induced diabetes C57BL/6J (STZ-C57) mice by oral infection of periodontal pathogenPorphyromonas gingivalisW50. We demonstrated that periodontal infected mice with high blood glucose levels showed dramatically more alveolar bone loss than their counterparts, in which infecteddb/dbmice exhibited the most bone defects. No contrary impact could be observed between this periodontal infection and onset and severity of diabetes. The expressions of PTPN2 were inhibited whereas the expression of JAK1, STAT1, and STAT3 increased dramatically in gingival epithelia and the serum TNF-αalso significantly increased in the mice with diabetic periodontitis. Our results indicated that the variations of inflammation-related protein expressions in gingival epithelia might lead to the phenotype differences in the mice with diabetic periodontitis.
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, China.
authors.forename: Qi
authors.identifier: 0000-0002-1588-9047
authors.initials: Q
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Center for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USA.
authors.forename: Ray
authors.initials: R
authors.lastname: Aprecio
authors.affiliation: Shandong Provincial Key Laboratory of Oral Tissue Regeneration, School of Stomatology, Shandong University, 44-1 Wenhua W. Road, Jinan, China.
authors.forename: Dongjiao
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Prosthodontics, The Affiliated Hospital of Stomatology, Guangxi Medical University, 10 Shuangyong Road, Nanning 530021, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Ji
authors.affiliation: Center for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USA.
authors.forename: Omaima
authors.initials: O
authors.lastname: Mohamed
authors.affiliation: Center for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USA.
authors.forename: Wu
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Center for Dental Research, School of Dentistry, Loma Linda University, 11175 Campus Street, Loma Linda, CA, USA.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, No. 14, 3rd Section S. Renmin Road, Chengdu, China.
authors.forename: Yi
authors.identifier: 0000-0002-0948-4991
authors.initials: Y
authors.lastname: Ding
mesh_terms: D016301:Alveolar Bone Loss; D000818:Animals; D001786:Blood Glucose / Q000378:metabolism*; D048909:Diabetes Complications / Q000209:etiology / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000150:complications / Q000378:metabolism*; D003922:Diabetes Mellitus, Type 1 / Q000150:complications / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000150:complications / Q000378:metabolism*; D004195:Disease Models, Animal; D005881:Gingiva / Q000378:metabolism; D053613:Janus Kinase 1 / Q000378:metabolism; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008815:Mice, Inbred Strains; D010518:Periodontitis / Q000209:etiology / Q000378:metabolism*; D016966:Porphyromonas gingivalis; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2 / Q000378:metabolism; D050794:STAT1 Transcription Factor / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D001786:Blood Glucose; D050794:STAT1 Transcription Factor; D050796:STAT3 Transcription Factor; C494080:Stat1 protein, mouse; C494087:Stat3 protein, mouse; D014409:Tumor Necrosis Factor-alpha; C507905:Jak1 protein, mouse; D053613:Janus Kinase 1; D054578:Protein Tyrosine Phosphatase, Non-Receptor Type 2; C516653:Ptpn2 protein, mouse