title: Identification and evaluation of a dominant alga from municipal wastewater in removal of nutrients.
abstract: To access better removal of nutrients with algae-based techniques, a dominant alga from real municipal wastewater was identified and its capacity in removing low concentrations of nitrogen (NH+4or NO-3) and phosphorus (PO3-4) was evaluated. Results showed that Oedogonium brevicingulatum, a filamentous green alga, was confirmed as the dominant alga in the secondary effluent of a municipal wastewater treatment plant by polymerase chain reaction-denaturing gradient gel electrophoresis. Low concentrations of NH+4or NO-3(≤5 mg N L-1) and PO3-4(≤0.5 mg P L-1) were 100% removed by the algae in a 7-d test. The maximum nutrient removal rate (Vmax) and the half-saturation constant (Km) for NH+4(10.03 ± 0.95 mg g-1d-1and 0.19 ± 0.03 mg L-1) and NO-3(8.43 ± 0.21 mg g-1d-1and 0.27 ± 0.11 mg L-1) indicated the uptake capability for NH+4is higher than that for NO-3. Meanwhile, it showed higher affinity for PO3-4(Vmax: 1.42 ± 0.02 mg g-1d-1; Km: 0.02 ± 0.00 mg L-1) with NH+4as nitrogen source than that (Vmax: 1.24 ± 0.15 mg g-1d-1; Km: 0.06 ± 0.03 mg L-1) with NO-3as nitrogen source. Moreover, nutrient removal efficiencies were observed steady when nitrogen/phosphorus ratio ranged from 5:1 to 20:1. These results suggest that the dominant algae from municipal wastewater have potentials to be applied in nutrient removal.
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Yixuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan 411105, China E-mail: gefei@xtu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Tang
authors.affiliation: Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan 411105, China E-mail: gefei@xtu.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Su
authors.affiliation: School of Environment and Energy, South China University of Technology, Guangzhou 510006, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Environmental Science and Engineering, College of Environment and Resources, Xiangtan University, Xiangtan 411105, China E-mail: gefei@xtu.edu.cn.
authors.forename: Fei
authors.initials: F
authors.lastname: Ge
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D000460:Chlorophyta / Q000378:metabolism*; D009566:Nitrates / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism*; D010710:Phosphates / Q000378:metabolism; D010758:Phosphorus / Q000378:metabolism*; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000032:analysis*; D014873:Water Pollutants / Q000032:analysis* / Q000378:metabolism*
chemical_list: D064751:Ammonium Compounds; D009566:Nitrates; D009584:Nitrogen; D010710:Phosphates; D010758:Phosphorus; D062065:Wastewater; D014873:Water Pollutants