title: Laser Induced breakdown spectroscopy: A rapid tool for the identification and quantification of minerals in cucurbit seeds.
abstract: Laser-induced breakdown spectroscopy (LIBS) was investigated to estimate the viability as a simple and rapid method for analysis of nutrient elements in seed kernels of cucurbits. LIBS spectra were recorded in the range of 200-975nm by using Q-switched Nd:YAG laser at 532nm (4ns, 10Hz) attached to echelle spectrometer with intensified charged coupled device (ICCD). The spectral analysis revealed the presence of several elements like C, O, N, Mg, Ca, Na and K in seeds. The quantification of elements (Mg, Ca, Na and K) through LIBS was done using calibration curve method in which all calibration curve shows good linearity (r>0.95). The result obtained through LIBS was in reasonable agreement with that obtained through atomic absorption spectroscopy (AAS). Principal Component Analysis (PCA) was also applied to the LIBS data for rapid categorization of seed samples belonging to same species although samples have similar nutrient elements.
authors.affiliation: Centre of Food Technology, University of Allahabad, Allahabad 211002, India. Electronic address: jyotsana30@gmail.com.
authors.forename: Jyotsana
authors.initials: J
authors.lastname: Singh
authors.affiliation: Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad 211002, India. Electronic address: rohit.02dec@gmail.com.
authors.forename: Rohit
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad 211002, India. Electronic address: awasthi.shi@gmail.com.
authors.forename: Shikha
authors.initials: S
authors.lastname: Awasthi
authors.affiliation: Centre of Food Technology, University of Allahabad, Allahabad 211002, India. Electronic address: vintis18@gmail.com.
authors.forename: Vinti
authors.initials: V
authors.lastname: Singh
authors.affiliation: Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad 211002, India. Electronic address: awadheshkrai@gmail.com.
authors.forename: A K
authors.initials: AK
authors.lastname: Rai
mesh_terms: D002138:Calibration; D028464:Cucurbita / Q000737:chemistry*; D007834:Lasers; D008903:Minerals / Q000032:analysis*; D012639:Seeds / Q000737:chemistry*; D013054:Spectrophotometry, Atomic / Q000379:methods*
chemical_list: D008903:Minerals
keywords: AAS; Cucurbits; ICCD; LIBS; PCA; Seeds