title: Detection of Receptor Heteromerization Using In Situ Proximity Ligation Assay.
abstract: Although G protein-coupled receptor (GPCR) heteromerization has been extensively demonstrated in vitro using heterologous cells that overexpress epitope-tagged receptors, their presence in endogenous systems is less well established. This is because a criterion to identify receptor heteromerization is the demonstration that the two interacting receptors are present not only in the same cell but also in the same subcellular compartment in close enough proximity to allow for direct receptor-receptor interaction. This has been difficult to study in native tissues due to a lack of sensitive and selective tools not only capable of detecting low-abundance proteins but also of demonstrating that they are in sufficiently close proximity to interact. The latter can be achieved using a proximity ligation assay (PLA). Detailed in this unit are protocols for demonstrating the presence of GPCR heteromers in endogenous cells as well as animal and human tissues, the controls required for these assays, and troubleshooting tips. © 2016 by John Wiley & Sons, Inc.
authors.affiliation: Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York.
authors.forename: Ivone
authors.initials: I
authors.lastname: Gomes
authors.affiliation: Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York.
authors.forename: Salvador
authors.initials: S
authors.lastname: Sierra
authors.affiliation: Department of Pharmacological Sciences, Icahn School of Medicine at Mount Sinai, New York, New York.
authors.forename: Lakshmi A
authors.initials: LA
authors.lastname: Devi
mesh_terms: D000818:Animals; D006801:Humans; D051379:Mice; D015336:Molecular Probe Techniques; D025941:Protein Interaction Mapping; D055503:Protein Multimerization; D043562:Receptors, G-Protein-Coupled / Q000032:analysis* / Q000737:chemistry*
chemical_list: D043562:Receptors, G-Protein-Coupled
keywords: G-protein coupled receptors; dimerization; in situ proximity ligation assay; receptor heteromers