title: Fermented Herbal Formulas KIOM-MA128 Ameliorate IL-6-Induced Intestinal Barrier Dysfunction in Colon Cancer Cell Line.
abstract: Inflammatory bowel disease (IBD) comprises Crohn's disease (CD) and ulcerative colitis (UC). IBD increases the risk of colorectal cancer (CRC), depending on the extent and duration of intestinal inflammation. Increased IL-6 expression has been reported in IBD patients, which may be associated with intestinal barrier function through discontinuous tight junction (TJ). KIOM-MA is a specific agent for allergic diseases and cancer, and it is composed of several plants; these herbs have been used in traditional oriental medicine. We fermented KIOM-MA, the product of KIOM-MA128, using probiotics to improve the therapeutic efficacy via the absorption and bioavailability of the active ingredients. In this study, we demonstrated that KIOM-MA/MA128 exhibited anticolitis effects via the modulation of TJ protein. Interleukin-6 resulted in a dose-dependent decrease in the TER and an increase in the FITC-dextran permeability; however, pretreatment with 400µg/ml KIOM-MA/MA128 resulted in a significant increase in the TER and a decrease in the FITC-dextran permeability via IL-6 induction. Furthermore, protein and mRNA TJ levels remained stable after pretreatment with 400µg/ml KIOM-MA/MA128. Moreover, KIOM-MA/MA128 suppressed the expression of PLCγ1 and PKC. Taken together, these findings suggest novel information and clue of the anticolitis effects of KIOM-MA128 via regulation of tight junction.
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.
authors.forename: Kwang Il
authors.initials: KI
authors.lastname: Park
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.
authors.forename: Dong Gun
authors.initials: DG
authors.lastname: Kim
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.
authors.forename: Bo Hyoung
authors.initials: BH
authors.lastname: Lee
authors.affiliation: Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine (KIOM), 70 Cheomdan-ro, Dong-gu, Daegu 41062, Republic of Korea.
authors.forename: Jin Yeul
authors.identifier: 0000-0001-8796-0394
authors.initials: JY
authors.lastname: Ma
mesh_terms: D015153:Blotting, Western; D018938:Caco-2 Cells; D002851:Chromatography, High Pressure Liquid; D003093:Colitis, Ulcerative / Q000378:metabolism; D003110:Colonic Neoplasms / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D017097:Electric Impedance; D005285:Fermentation; D006801:Humans; D015850:Interleukin-6 / Q000494:pharmacology*; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D062725:Tight Junction Proteins / Q000235:genetics / Q000378:metabolism; D019108:Tight Junctions / Q000187:drug effects
chemical_list: D004365:Drugs, Chinese Herbal; D015850:Interleukin-6; D062725:Tight Junction Proteins