title: ESI-MS2and Anti-inflammatory Studies of Cyclopropanic Triterpenes. UPLC-ESI-MS and MS2Search of Related Metabolites from Donella ubanguiensis.
abstract: BACKGROUND
  Triterpenes are one of the largest secondary metabolites groups spread in the plant kingdom with various skeletons. These metabolites have showed various bioactivities including anti-inflammatory activity.
  
  
  OBJECTIVE
  The study aims to explore the mass spectrometry fragmentation of donellanic acids A-C (DA A-C), three compounds identified from Donella ubanguiensis; in addition, the fragmentation behaviour of these metabolites will serve as a fingerprint to search and characterise triterpenes congeners in fruits, bark and wood crude extracts of D. ubanguiensis. This work was prompted by the anti-inflammatory activity on leukocyte migration, exudate concentrations and myeloperoxidase activity obtained for DA A-B.
  
  
  METHODS
  The bioactivity was performed on mouse model of pleurisy induced by carrageenan and the parameters were analysed by veterinarian automated cell counter and colorimetric assays. While the tandem mass analyses of DA A-C were carried out by a direct infusion ESI-QTOF-MS/MS, the extracts were studied by UPLC-ESI-QTOF-MS and UPLC-ESI-QTOF-MS/MS.
  
  
  RESULTS
  DA A displayed interesting anti-inflammatory activity by inhibiting leukocyte migration, exudate concentrations and myeloperoxidase activity (p < 0.05) while DA B was weakly active (p > 0.05). Moreover, the diagnostic of the MS2behaviour of DA A-C in conjunction with the chromatograms and the obtained MS2data of the crude extract led to the characterisation of three cyclopropane triterpenes (T1-T3) and six saponins (T4-T9) from the fruits, the bark, and the wood extracts.
  
  
  CONCLUSIONS
  Donella species deserve more investigation since metabolites related to the anti-inflammatory compound (DA A) could be identified. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis, 88040-970, SC, Brazil.
authors.forename: Louis P
authors.initials: LP
authors.lastname: Sandjo
authors.affiliation: Department of Clinical Analysis, Centre of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis, 88040-970, SC, Brazil.
authors.forename: Marcus V P Dos Santos
authors.initials: MV
authors.lastname: Nascimento
authors.affiliation: Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis, 88040-970, SC, Brazil.
authors.forename: Layzon A L
authors.initials: LA
authors.lastname: da Silva
authors.affiliation: Department of Clinical Analysis, Centre of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis, 88040-970, SC, Brazil.
authors.forename: Antonio C M
authors.initials: AC
authors.lastname: Munhoz
authors.affiliation: Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis, 88040-970, SC, Brazil.
authors.forename: Luiz A E
authors.initials: LA
authors.lastname: Pollo
authors.affiliation: Department of Pharmaceutical Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis, 88040-970, SC, Brazil.
authors.forename: Maique W
authors.initials: MW
authors.lastname: Biavatti
authors.affiliation: Department of Organic Chemistry, Faculty of Science, University of Yaoundé 1, P.O. Box 812, Yaoundé, Cameroon.
authors.forename: Bonaventure T
authors.initials: BT
authors.lastname: Ngadjui
authors.affiliation: Institute of Organic Chemistry, Johannes Gutenberg-University of Mainz, Duesbergweg 10-14, 55128, Mainz, Germany.
authors.forename: Till
authors.initials: T
authors.lastname: Opatz
authors.affiliation: Department of Clinical Analysis, Centre of Health Sciences, Universidade Federal de Santa Catarina, Campus Universitário - Trindade, Florianópolis, 88040-970, SC, Brazil.
authors.forename: Tania S
authors.initials: TS
authors.lastname: Fröde
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000032:analysis* / Q000494:pharmacology* / Q000627:therapeutic use; D002634:Chemotaxis, Leukocyte / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid; D003521:Cyclopropanes / Q000737:chemistry*; D005260:Female; D019684:Magnoliopsida / Q000378:metabolism*; D051379:Mice; D009195:Peroxidase / Q000037:antagonists & inhibitors; D010998:Pleurisy / Q000139:chemically induced / Q000188:drug therapy; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D014315:Triterpenes / Q000032:analysis* / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D000893:Anti-Inflammatory Agents; D003521:Cyclopropanes; D014315:Triterpenes; C030797:cyclopropane; D009195:Peroxidase
keywords: LC-MS; LC-MS/MS; MS/MS; anti-inflammatory activity; cyclopropanic triterpenes