title: Association of Reduced Heme Oxygenase-1 with Decreased MicroRNA-196a2 Expression in Peripheral Blood Mononuclear Cells of Patients with Intestinal Behcet's Disease.
abstract: OBJECTIVE
  To examine the expression of miRNAs and mRNAs of heme oxygenase 1 (HO-1) in patients with active intestinal Behcet's disease (BD).
  
  
  METHODS
  Peripheral blood mononuclear cells (PBMCs) were obtained from BD patients with active ileocecal ulcers or from healthy donors. Expression levels of four miRNAs were studied using real-time PCR. The levels of mRNAs of ho-1, bach1, and CD14 were measured by qRT-PCR. Serum levels of cytokines were analyzed by ELISA.
  
  
  RESULTS
  Among four miRNAs, only levels of miR-196a2 were significantly decreased from BD patients with active ileocecal ulcers as compared with healthy controls. Moreover, level of mRNA ho-1 expression in PBMCs from patients with BD was reduced. No significant difference on bach1 and CD14 mRNA levels was observed. Levels of IFN-γ, IL-17, IL-10, IL-1β, and TNF-α were higher in patients with active intestinal BD than those in healthy controls.
  
  
  CONCLUSIONS
  The present results suggest that miR-196a2 expression is decreased in active intestinal BD patients. Down regulated miR-196a2 may be involved in intestinal BD pathogenesis by targeting Bach1/ho-1. Consequently, pro-inflammatory cytokines are closely implicated in the evolution of intestinal BD.
authors.affiliation: Department of Immunology and Rheumatology, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.affiliation: Center of Gastrointestinal Endoscopy, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Da-Nian
authors.initials: DN
authors.lastname: Ji
authors.affiliation: Department of Immunology and Rheumatology, Huadong Hospital, Fudan University, Shanghai, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Department of Immunology and Rheumatology, Huadong Hospital, Fudan University, Shanghai, China guanjianlong@medmail.com.cn songbai1009@163.com.|Shanghai Key Laboratory of Clinical Geriatric Medicine, Research Center on Aging and Medicine, Fudan University, Shanghai, China.
authors.forename: Jian-Long
authors.initials: JL
authors.lastname: Guan
authors.affiliation: Department of Gastroenterology, Huadong Hospital, Fudan University, Shanghai, China guanjianlong@medmail.com.cn songbai1009@163.com.|Shanghai Key Laboratory of Clinical Geriatric Medicine, Research Center on Aging and Medicine, Fudan University, Shanghai, China.
authors.forename: Song-Bai
authors.initials: SB
authors.lastname: Zheng
mesh_terms: D000328:Adult; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D001528:Behcet Syndrome / Q000097:blood / Q000201:enzymology* / Q000235:genetics*; D016207:Cytokines / Q000097:blood; D051856:Fanconi Anemia Complementation Group Proteins / Q000235:genetics / Q000378:metabolism; D005260:Female; D005786:Gene Expression Regulation; D051547:Heme Oxygenase-1 / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: C102108:BACH1 protein, human; D050976:Basic-Leucine Zipper Transcription Factors; D016207:Cytokines; D051856:Fanconi Anemia Complementation Group Proteins; C542543:MIRN196 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D051547:Heme Oxygenase-1
keywords: Bach1; Behcet’s disease (BD); heme oxygenase-1 (HO-1); ileocolonic ulcer; microRNA (miRNA)