title: Pathway Construction in Corynebacterium glutamicum and Strain Engineering To Produce Rare Sugars from Glycerol.
abstract: Rare sugars are valuable natural products widely used in pharmaceutical and food industries. In this study, we expected to synthesize rare ketoses from abundant glycerol using dihydroxyacetone phosphate (DHAP)-dependent aldolases. First, a new glycerol assimilation pathway was constructed to synthesize DHAP. The enzymes which convert glycerol to 3-hydroxypropionaldehyde and l-glyceraldehyde were selected, and their corresponding aldehyde synthesis pathways were constructed in vivo. Four aldol pathways based on different aldolases and phosphorylase were gathered. Next, three pathways were assembled and the resulting strains synthesized 5-deoxypsicose, 5-deoxysorbose, and 5-deoxyfructose from glucose and glycerol and produce l-fructose, l-tagatose, l-sorbose, and l-psicose with glycerol as the only carbon source. To achieve higher product titer and yield, the recombinant strains were further engineered and fermentation conditions were optimized. Fed-batch culture of engineered strains obtained 38.1 g/L 5-deoxypsicose with a yield of 0.91 ± 0.04 mol product per mol of glycerol and synthesized 20.8 g/L l-fructose, 10.3 g/L l-tagatose, 1.2 g/L l-sorbose, and 0.95 g/L l-psicose.
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Jiangang
authors.identifier: http://orcid.org/0000-0001-9463-8862
authors.initials: J
authors.lastname: Yang
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Yueming
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Men
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Shangshang
authors.initials: S
authors.lastname: Sun
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Yuanxia
authors.initials: Y
authors.lastname: Sun
authors.affiliation: National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences , Tianjin 300308, China.
authors.forename: Yanhe
authors.initials: Y
authors.lastname: Ma
mesh_terms: D000446:Aldehyde-Lyases / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D018533:Biomass; D053898:Biosynthetic Pathways; D002851:Chromatography, High Pressure Liquid; D048230:Corynebacterium glutamicum / Q000235:genetics / Q000378:metabolism*; D004926:Escherichia coli / Q000235:genetics; D005285:Fermentation; D005632:Fructose / Q000096:biosynthesis; D005947:Glucose / Q000378:metabolism; D005985:Glyceraldehyde / Q000031:analogs & derivatives / Q000378:metabolism; D005990:Glycerol / Q000378:metabolism*; D006601:Hexoses / Q000096:biosynthesis; D006836:Hydro-Lyases / Q000378:metabolism; D007661:Ketoses / Q000096:biosynthesis*; D009682:Magnetic Resonance Spectroscopy; D060847:Metabolic Engineering; D011407:Propane / Q000378:metabolism; D013013:Sorbose / Q000096:biosynthesis
chemical_list: D006601:Hexoses; D007661:Ketoses; C047158:3-hydroxypropionaldehyde; C003243:psicose; D005632:Fructose; D005985:Glyceraldehyde; D000446:Aldehyde-Lyases; D006836:Hydro-Lyases; C020671:glycerol dehydratase; D005947:Glucose; D013013:Sorbose; D005990:Glycerol; D011407:Propane; C030192:tagatose
keywords: Corynebacterium glutamicum; aldolases; glycerol; metabolic engineering; rare sugars