title: Selective Visualization of the Endogenous Peroxynitrite in an Inflamed Mouse Model by a Mitochondria-Targetable Two-Photon Ratiometric Fluorescent Probe.
abstract: Peroxynitrite (ONOO-) is a kind of reactive oxygen species (ROS) with super activity of oxidization and nitration, and overproduction of ONOO-is associated with pathogenesis of many diseases. Thus, accurate detection of ONOO-with high sensitivity and selectivity is imperative for elucidating its functions in health or disease states. Herein we for the first time present a new two-photon ratiometric fluorescent ONOO-probe (MITO-CC) based on FRET mechanism by combining rational design strategy and dye-screening approach. MITO-CC, with fast response rate (within 20 s), excellent sensitivity (detection limit = 11.30 nM) and outstanding selectivity toward ONOO-, was successfully applied to ratiometric detection of endogenous ONOO-produced by HepG2/RAW264.7 cells and further employed for imaging oxidative stress in an inflamed mouse model. Therefore, probe MITO-CC could be a potential biological tool to explore the roles of ONOO-under different physiological and pathological settings.
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, P. R. China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore , Singapore 117543, Singapore.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, P. R. China.
authors.forename: Zebing
authors.identifier: 0000-0002-9788-1988
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, P. R. China.
authors.forename: Lin
authors.identifier: 0000-0002-1015-5319
authors.initials: L
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University , Changsha 410082, P. R. China.
authors.forename: Xiaobing
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Medicinal Chemistry Programme, National University of Singapore , Singapore 117543, Singapore.|Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium , Singapore 117543, Singapore.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Chang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D031541:Fluorescence Resonance Energy Transfer; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D007249:Inflammation / Q000473:pathology*; D051379:Mice; D008815:Mice, Inbred Strains; D008928:Mitochondria / Q000737:chemistry*; D018384:Oxidative Stress; D030421:Peroxynitrous Acid / Q000032:analysis*; D017785:Photons; D000067996:RAW 264.7 Cells
chemical_list: D005456:Fluorescent Dyes; D030421:Peroxynitrous Acid