title: Comparative evaluation of sutures coated with triclosan and chlorhexidine for oral biofilm inhibition potential and antimicrobial activity against periodontal pathogens: Anin vitrostudy.
abstract: BACKGROUND
  Surgical site plaque accumulation is one of the challenging problems leading to unfavorable healing. The antibacterial sutures can be used to reduce or inhibit plaque formation. Presently there is no study comparing efficacy of sutures coated with triclosan and chlorhexidine in terms of oral biofilm inhibition and antimicrobial property against periodontal pathogens.
  
  
  OBJECTIVE
  The aim of present study was to evaluate the antibacterial efficacy and oral biofilm inhibition around chlorhexidine and triclosan coated polyglactin sutures in comparison to uncoated sutures.
  
  
  METHODS
  Equal segments of chlorhexidine and triclosan coated polyglactin sutures (3-0) were incubated at 370°C in saliva collected from 10 chronic periodontitis patients for 7 days. Plain uncoated suture served as control. Biofilm formation was analyzed with Confocal Laser-Scanning Microscopy (CLSM) and Scanning Electron Microscopy (SEM). Quantitative assessment was done using Colony Forming Units (CFU/mL).The antibacterial efficacy of the sutures was tested against specific periodontal pathogens (S.mutans, F.nucleatum, A.actinomycetomcomitans, P.intermedia, P.gingivalis) using agar diffusion method. CLSM and SEM were not subjected to statistical analysis. ANOVA test was used for colony forming units and agar diffusion test. (P < 0.05) Results: CLSM and SEM showed substantial biofilm inhibition around chlorhexidine-coated sutures followed by triclosan-coated when compared to plain uncoated suture. The antibacterial coated sutures showed statistically significant difference in CFUs/ml and zone of inhibition compared to plain uncoated sutures. Among coated sutures, chlorhexidine-coated sutures showed better results.
  
  
  CONCLUSIONS
  The antibacterial coated sutures have a promising potential in preventing the colonization of periodontal pathogens around it thereby inhibiting biofilm formation.
authors.affiliation: Department of Periodontics, MGV's KBH Dental College and Hospital, Nashik, Maharashtra, India.
authors.forename: Kunal Sunder
authors.initials: KS
authors.lastname: Sethi
authors.affiliation: Department of Periodontics, MGV's KBH Dental College and Hospital, Nashik, Maharashtra, India.
authors.forename: Prerna Ashok
authors.initials: PA
authors.lastname: Karde
authors.affiliation: Department of Periodontics, MGV's KBH Dental College and Hospital, Nashik, Maharashtra, India.
authors.forename: Chaitanya Pradeep
authors.initials: CP
authors.lastname: Joshi
mesh_terms: D000891:Anti-Infective Agents, Local / Q000627:therapeutic use*; D018441:Biofilms / Q000187:drug effects*; D002710:Chlorhexidine / Q000627:therapeutic use*; D006801:Humans; D066298:In Vitro Techniques; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010518:Periodontitis / Q000382:microbiology / Q000517:prevention & control; D013537:Sutures / Q000382:microbiology; D014260:Triclosan / Q000627:therapeutic use*
chemical_list: D000891:Anti-Infective Agents, Local; D014260:Triclosan; D002710:Chlorhexidine