title: Data supporting regulating temporospatial dynamics of morphogen for structure formation of the lacrimal gland by chitosan biomaterials.
abstract: The lacrimal gland is responsible for tear synthesis and secretion, and is derived from the epithelia of ocular surface and generated by branching morphogenesis. The dataset presented in this article is to support the research results of the effect of chitosan biomaterials on facilitating the structure formation of the lacrimal gland by regulating temporospatial dynamics of morphogen. The embryonic lacrimal gland explants were used as the standard experimental model for investigating lacrimal gland branching morphogenesis. Chitosan biomaterials promoted lacrimal gland branching with a dose-dependent effect. It helpedin vivobinding of hepatocyte growth factor (HGF) related molecules in the epithelial-mesenchymal boundary of emerging epithelial branches. When mitogen-activated protein kinase (MAPK) or protein kinase B (Akt/PKB) inhibitors applied, the chitosan effects reduced. Nonetheless, the ratios of MAPK and Akt/PKB phosphorylation were still greater in the chitosan group than the control. The data demonstrated here confirm the essential role of HGF-signaling in chitosan-promoted structure formation of the lacrimal gland.
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan, ROC; Department of Ophthalmology, Zhongxing Branch, Taipei City Hospital, Taipei, Taiwan, ROC; Department of Ophthalmology, College of Medicine, National Yang-Ming University, Taipei, Taiwan, ROC.
authors.forename: Ya-Chuan
authors.initials: YC
authors.lastname: Hsiao
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, Taipei, Taiwan, ROC; Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, Taipei, Taiwan, ROC; Graduate Institute of Clinical Medicine, College of Medicine, National Taiwan University, Taipei 10002, Taiwan, ROC.
authors.forename: Tsung-Lin
authors.initials: TL
authors.lastname: Yang
keywords: Branching morphogenesis; Chitosan; Hepatocyte growth factor; Lacrimal gland