title: Identification and Characterization of Ana o 3 Modifications on Arginine-111 Residue in Heated Cashew Nuts.
abstract: Raw and roasted cashew nut extracts were evaluated for protein modifications by mass spectrometry. Independent modifications on the Arg-111 residue of Ana o 3 were observed in roasted but not raw cashew nuts. The mass changes of 72.0064 or 53.9529 Da are consistent with the formation of carboxyethyl and hydroimidazolone modifications at the Arg-111 residue. These same modifications were observed in Ana o 3 purified from roasted but not raw cashew nuts, albeit at a relatively low occurrence. Circular dichroism indicated that Ana o 3 purified from raw and roasted cashew nuts had similar secondary structure, and dynamic light scattering analysis indicated there was no observable difference in particle size. The stability of Ana o 3 purified from raw and roasted cashew nuts to trypsin was similar in the absence of or following treatment with a reducing agent. Only minor differences in IgE binding to Ana o 3 were observed by ELISA among a cohort of cashew-allergic patient sera.
authors.affiliation: Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture , 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
authors.forename: Christopher P
authors.identifier: http://orcid.org/0000-0001-7819-0914
authors.initials: CP
authors.lastname: Mattison
authors.affiliation: Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture , 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
authors.forename: Casey C
authors.initials: CC
authors.lastname: Grimm
authors.affiliation: Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture , 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Li
authors.affiliation: BioMed Bridge, LLC , 3700 Quebec Street, Suite 100-230, Denver, Colorado 80207, United States.
authors.forename: Heidi J
authors.initials: HJ
authors.lastname: Chial
authors.affiliation: Biophysics Instrumentation Facility, Biochemistry Department, University of Wisconsin , 433 Babcock Drive, Madison, Wisconsin 53706, United States.
authors.forename: Darrell R
authors.initials: DR
authors.lastname: McCaslin
authors.affiliation: Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture , 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
authors.forename: Si-Yin
authors.initials: SY
authors.lastname: Chung
authors.affiliation: BioMed Bridge, LLC , 3700 Quebec Street, Suite 100-230, Denver, Colorado 80207, United States.
authors.forename: Yvette
authors.initials: Y
authors.lastname: Bren-Mattison
authors.affiliation: Allergy Partners of North Texas Research, Department of Pediatrics, Medical City Children's Hospital , 7777 Forest Lane, Dallas, Texas 75230, United States.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Wasserman
mesh_terms: D031021:Anacardium / Q000737:chemistry*; D052179:Antigens, Plant / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D001120:Arginine / Q000737:chemistry*; D002942:Circular Dichroism; D000067493:Dynamic Light Scattering; D004797:Enzyme-Linked Immunosorbent Assay; D005511:Food Handling / Q000379:methods*; D006361:Heating; D006801:Humans; D007093:Imidazoles / Q000737:chemistry; D007106:Immune Sera; D007073:Immunoglobulin E / Q000378:metabolism; D021184:Nut Hypersensitivity / Q000276:immunology; D009754:Nuts / Q000737:chemistry; D010316:Particle Size; D010940:Plant Proteins / Q000737:chemistry* / Q000302:isolation & purification / Q000378:metabolism; D011487:Protein Conformation; D014357:Trypsin / Q000737:chemistry
chemical_list: C502483:Ana o 3 protein, Anacardium occidentale; D052179:Antigens, Plant; D007093:Imidazoles; D007106:Immune Sera; D010940:Plant Proteins; C117197:imidazolone; D007073:Immunoglobulin E; D001120:Arginine; D014357:Trypsin
keywords: Ana o 3; cashew; food allergy; heating; modification