title: Sequential analysis of 18 genes in polycythemia vera and essential thrombocythemia reveals an association between mutational status and clinical outcome.
abstract: Philadelphia-negative classical myeloproliferative neoplasms (MPN) are clonal diseases characterized by driver mutations of JAK2, MPL, or CALR. Additional mutations may occur in epigenetic regulators, signaling, or splicing genes that may be useful in the prognostic assessment of MPN patients. In primary myelofibrosis, molecular-based prognostic scoring systems have been recently proposed, but few data are available to date for polycythemia vera (PV) and essential thrombocythemia (ET). In this study, we used a next generation sequencing-based 18-gene panel in 50 JAK2V617F positive PV and JAK2V617F positive ET patients from an institutional cohort investigated at diagnosis and at 3-year follow-up (3y). Disease progression at 3y was defined by a composite criterion. Patients (28 PV and 22 ET) were included according to their clinical status, with or without disease progression. At diagnosis, we found 28 additional mutations in 21 of the 50 patients. Patients with disease progression were more likely to have at least one additional mutation. There was no difference between PV and ET. All patients with two or more additional mutations exhibited disease progression at 3y. No novel mutations appeared at 3y. The allele burden increase by at least one mutation at 3y was more frequent in patients with disease progression. Our data suggest that screening for additional mutations in PV and ET could identify patients at a higher risk of disease progression. © 2017 Wiley Periodicals, Inc.
authors.affiliation: CHU Angers, Laboratoire d'Hématologie, Angers, France.|Université d'Angers, UFR Santé, Angers, France.|INSERM Unité 892, CNRS Unit 6299, Angers, France.|Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).
authors.forename: Damien
authors.initials: D
authors.lastname: Luque Paz
authors.affiliation: Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).|CHU Brest, Laboratoire d'Hématologie, Brest, France.|Université de Brest, Bretagne Occidentale, UFR Médecine, Brest, France.|INSERM Unité 1078, CHRU Brest, Brest, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Chauveau
authors.affiliation: Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).|CHU Angers, Service des Maladies du Sang, Angers, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Boyer
authors.affiliation: Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).|CHU Brest, Laboratoire d'Hématologie, Brest, France.
authors.forename: Caroline
authors.initials: C
authors.lastname: Buors
authors.affiliation: INSERM Unité 1078, CHRU Brest, Brest, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Samaison
authors.affiliation: CHU Angers, Laboratoire d'Hématologie, Angers, France.|Université d'Angers, UFR Santé, Angers, France.|INSERM Unité 892, CNRS Unit 6299, Angers, France.|Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).
authors.forename: Laurane
authors.initials: L
authors.lastname: Cottin
authors.affiliation: Université d'Angers, UFR Santé, Angers, France.|INSERM Unité 892, CNRS Unit 6299, Angers, France.|Département de Statistique, Institut de Cancérologie de l'Ouest, Angers, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Seegers
authors.affiliation: Université de Brest, Bretagne Occidentale, UFR Médecine, Brest, France.|INSERM Unité 1078, CHRU Brest, Brest, France.|CHU Brest, Laboratoire de Génétique, Brest, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Férec
authors.affiliation: Université de Brest, Bretagne Occidentale, UFR Médecine, Brest, France.|INSERM Unité 1078, CHRU Brest, Brest, France.|CHU Brest, Laboratoire de Génétique, Brest, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Le Maréchal
authors.affiliation: Université de Brest, Bretagne Occidentale, UFR Médecine, Brest, France.|INSERM Unité 1078, CHRU Brest, Brest, France.|CHU Brest, Laboratoire de Génétique, Brest, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Gueguen
authors.affiliation: Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).|CHU Brest, Laboratoire d'Hématologie, Brest, France.|Université de Brest, Bretagne Occidentale, UFR Médecine, Brest, France.|INSERM Unité 1078, CHRU Brest, Brest, France.
authors.forename: Eric
authors.initials: E
authors.lastname: Lippert
authors.affiliation: Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).|CHU Brest, Service d'Hématologie Clinique, Brest, France.
authors.forename: Jean-Christophe
authors.initials: JC
authors.lastname: Ianotto
authors.affiliation: CHU Angers, Laboratoire d'Hématologie, Angers, France.|Université d'Angers, UFR Santé, Angers, France.|INSERM Unité 892, CNRS Unit 6299, Angers, France.|Fédération Hospitalo-Universitaire 'Grand Ouest Against Leukemia' (FHU GOAL).
authors.forename: Valérie
authors.initials: V
authors.lastname: Ugo
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D015331:Cohort Studies; D018450:Disease Progression; D005500:Follow-Up Studies; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods; D006801:Humans; D053614:Janus Kinase 2 / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D011087:Polycythemia Vera / Q000235:genetics* / Q000473:pathology*; D011379:Prognosis; D013920:Thrombocythemia, Essential / Q000235:genetics* / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C507924:JAK2 protein, human; D053614:Janus Kinase 2