title: Quantitative evaluation of atherosclerotic plaques using cross-polarization optical coherence tomography, nonlinear, and atomic force microscopy.
abstract: A combination of approaches to the image analysis in cross-polarization optical coherence tomography (CP OCT) and high-resolution imaging by nonlinear microscopy and atomic force microscopy (AFM) at the different stages of atherosclerotic plaque development is studied. This combination allowed us to qualitatively and quantitatively assess the disorganization of collagen in the atherosclerotic arterial tissue (reduction and increase of CP backscatter), at the fiber (change of the geometric distribution of fibers in the second-harmonic generation microscopy images) and fibrillar (violation of packing and different nature of a basket-weave network of fibrils in the AFM images) organization levels. The calculated CP channel-related parameters are shown to have a statistically significant difference between stable and unstable (also called vulnerable) plaques, and hence, CP OCT could be a potentially powerful, minimally invasive method for vulnerable plaques detection.
authors.affiliation: Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Gubarkova
authors.affiliation: Institute of Applied Physics RAS, 46 Ulyanov Street, Nizhny Novgorod 603950, Russia.
authors.forename: Mikhail Yu
authors.initials: MY
authors.lastname: Kirillin
authors.affiliation: Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, RussiacN.I. Lobachevsky State University of Nizhny Novgorod, 23 Gagarin Avenue, Nizhny Novgorod 603950, Russia.
authors.forename: Varvara V
authors.initials: VV
authors.lastname: Dudenkova
authors.affiliation: Institute of Photonic Technologies, Research Center of Crystallography and Photonics RAS, 2 Pionerskaya Street, Troitsk, Moscow 142190, RussiaeI.M. Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya Street, Moscow 119991, Russia.
authors.forename: Peter S
authors.initials: PS
authors.lastname: Timashev
authors.affiliation: N.N. Semenov Institute of Chemical Physics, 4 Kosygin Street, Moscow 119991, Russia.
authors.forename: Svetlana L
authors.initials: SL
authors.lastname: Kotova
authors.affiliation: Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, Russia.
authors.forename: Elena B
authors.initials: EB
authors.lastname: Kiseleva
authors.affiliation: Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, Russia.
authors.forename: Lidia B
authors.initials: LB
authors.lastname: Timofeeva
authors.affiliation: N.N. Semenov Institute of Chemical Physics, 4 Kosygin Street, Moscow 119991, Russia.
authors.forename: Galina V
authors.initials: GV
authors.lastname: Belkova
authors.affiliation: N.N. Semenov Institute of Chemical Physics, 4 Kosygin Street, Moscow 119991, Russia.
authors.forename: Anna B
authors.initials: AB
authors.lastname: Solovieva
authors.affiliation: Institute of Applied Physics RAS, 46 Ulyanov Street, Nizhny Novgorod 603950, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Moiseev
authors.affiliation: Institute of Applied Physics RAS, 46 Ulyanov Street, Nizhny Novgorod 603950, Russia.
authors.forename: Gregory V
authors.initials: GV
authors.lastname: Gelikonov
authors.affiliation: Institute of Applied Physics RAS, 46 Ulyanov Street, Nizhny Novgorod 603950, Russia.
authors.forename: Ilya I
authors.initials: II
authors.lastname: Fiks
authors.affiliation: Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, Russia.
authors.forename: Felix I
authors.initials: FI
authors.lastname: Feldchtein
authors.affiliation: Nizhny Novgorod State Medical Academy, 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603950, Russia.
authors.forename: Natalia D
authors.initials: ND
authors.lastname: Gladkova
mesh_terms: D000465:Algorithms; D003094:Collagen; D003331:Coronary Vessels / Q000000981:diagnostic imaging; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D018625:Microscopy, Atomic Force / Q000379:methods*; D058226:Plaque, Atherosclerotic / Q000000981:diagnostic imaging*; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D003094:Collagen