title: Eliciting 10E8-like antibodies by the membrane proximal external region peptide of HIV-1 in guinea pigs.
abstract: OBJECTIVE
  To develop an immunotherapy for HIV that can elicit 10E8-like broadly-neutralizing antibodies in guinea pigs, using a multiple antigen peptide (MAP) system as the platform and 10E8 peptide as the epitope.
  
  
  RESULTS
  The immunogen, 10E8-MAP4, was synthetized using the MAP system. The synthetic 10E8-MAP4was stable, and the epitopes could be exposed for recognition. In addition, the 10E8 epitope was present in an α-helical structure, which was hypothesized to aid in the generation of neutralizing antibodies. In vivo analysis showed that 10E8-MAP4could efficiently elicit HIV binding antibodies in guinea pigs, although only weak neutralizing activities were observed.
  
  
  CONCLUSIONS
  Multiple antigen peptide is an excellent vaccine platform for generating binding antibodies, but may elicit weak neutralizing antibodies for HIV.
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: Yongjiao
authors.initials: Y
authors.lastname: Yu
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Fu
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Gong
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Guan
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: Xiaoqiu
authors.initials: X
authors.lastname: He
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: He
authors.initials: H
authors.lastname: Yin
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Kuai
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.|Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, School of Life Sciences, Jilin University, Changchun, Jilin, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Kong
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Shi
authors.affiliation: National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, No. 2699 Qianjin Street, Changchun, 130012, Jilin, China. shanym@jlu.edu.cn.
authors.forename: Yaming
authors.initials: Y
authors.lastname: Shan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000378:metabolism; D057134:Antibodies, Neutralizing / Q000097:blood; D002462:Cell Membrane / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D002942:Circular Dichroism; D004797:Enzyme-Linked Immunosorbent Assay; D006168:Guinea Pigs; D015483:HIV Antibodies / Q000276:immunology*; D015497:HIV-1 / Q000276:immunology*; D007114:Immunization; D009500:Neutralization Tests; D010455:Peptides / Q000737:chemistry / Q000276:immunology*; D011485:Protein Binding
chemical_list: D000911:Antibodies, Monoclonal; D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D010455:Peptides
keywords: 10E8 epitope; Broadly-neutralizing antibody; HIV-1 vaccine; Multiple antigen peptide; Vaccine for HIV