title: Recombinant Marek's disease virus type 1 provides full protection against very virulent Marek's and infectious bursal disease viruses in chickens.
abstract: Marek's disease virus (MDV) is a preferred vector in the construction of recombinant vaccines. However, bivalent vaccine based on MDV that confers full protection against both very virulent Marek's and infectious bursal disease virus (IBDV) infections in chickens has not been produced. Here we developed a system utilizing overlapping fosmid DNAs transfection that rescues an MDV type 1 (MDV1) vaccine strain. Using this system, we inserted the IBDV VP2 gene at MDV1 genome sites UL41, US10 and US2. The VP2 protein was stably expressed in the recombinant MDV-infected cells and self-assembled into IBDV subviral particles. Insertion of the VP2 gene did not affect the replication phenotype of MDV in cell cultures, nor did it increase the virulence of the MDV vaccine strain in chickens. After challenge with very virulent IBDV, r814US2VP2 conferred full protection, whereas r814UL41VP2 and r814US10VP2 provided partial or no protection. All the three recombinant vaccines provided full protection against very virulent MDV challenge in chickens. These results demonstrated that r814US2VP2 could be used as a promising bivalent vaccine against both Marek's and infectious bursal diseases in chickens.
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Li
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Yongzhen
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Changjun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Cui
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Xiaole
authors.initials: X
authors.lastname: Qi
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Avian Immunosuppressive Diseases Division, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, P.R. China.
authors.forename: Xiaomei
authors.initials: X
authors.lastname: Wang
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000032:analysis / Q000276:immunology; D018175:Birnaviridae Infections / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D002460:Cell Line; D002645:Chickens; D004797:Enzyme-Linked Immunosorbent Assay; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D016679:Genome, Viral; D008381:Herpesvirus 2, Gallid / Q000235:genetics* / Q000472:pathogenicity; D007243:Infectious bursal disease virus / Q000235:genetics / Q000472:pathogenicity; D008380:Marek Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D008854:Microscopy, Electron; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011994:Recombinant Proteins / Q000096:biosynthesis / Q000235:genetics; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D015678:Viral Structural Proteins / Q000235:genetics / Q000378:metabolism; D014765:Viral Vaccines / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D014774:Virulence
chemical_list: D000914:Antibodies, Viral; D011994:Recombinant Proteins; C082513:VP2 protein, infectious bursal disease virus; D014614:Vaccines, Synthetic; D015678:Viral Structural Proteins; D014765:Viral Vaccines