title: Dicer-Like 4 Is Involved in Restricting the Systemic Movement of Zucchini yellow mosaic virus in Nicotiana benthamiana.
abstract: Zucchini yellow mosaic virus (ZYMV) induces serious diseases in cucurbits. To create a tool to screen for resistance genes, we cloned a wild ZYMV isolate and inserted the visual marker Rosea1 to obtain recombinant clone ZYMV-Ros1. While in some plant-virus combinations Rosea1 induces accumulation of anthocyanins in infected tissues, ZYMV-Ros1 infection of cucurbits did not lead to detectable anthocyanin accumulation. However, the recombinant virus did induce dark red pigmentation in infected tissues of the model plant Nicotiana benthamiana. In this species, ZYMV-Ros1 multiplied efficiently in local inoculated tissue but only a few progeny particles established infection foci in upper leaves. We used this system to analyze the roles of Dicer-like (DCL) genes, core components of plant antiviral RNA silencing pathways, in ZYMV infection. ZYMV-Ros1 local replication was not significantly affected in single DCL knockdown lines nor in double DCL2/4 and triple DCL2/3/4 knockdown lines. ZYMV-Ros1 systemic accumulation was not affected in knockdown lines DCL1, DCL2, and DCL3. However in DCL4 and also in DCL2/4 and DCL2/3/4 knockdown lines, ZYMV-Ros1 systemic accumulation dramatically increased, which highlights the key role of DCL4 in restricting virus systemic movement. The effect of DCL4 on ZYMV systemic movement was confirmed with a wild-type version of the virus.
authors.affiliation: 1 Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), 46022 Valencia, Spain; and.
authors.forename: Teresa
authors.initials: T
authors.lastname: Cordero
authors.affiliation: 1 Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), 46022 Valencia, Spain; and.
authors.forename: Lidia
authors.initials: L
authors.lastname: Cerdán
authors.affiliation: 1 Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), 46022 Valencia, Spain; and.
authors.forename: Alberto
authors.initials: A
authors.lastname: Carbonell
authors.affiliation: 2 Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology; and Department of Biology, University of Crete, Heraklion, Crete, Greece.
authors.forename: Konstantina
authors.initials: K
authors.lastname: Katsarou
authors.affiliation: 2 Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology; and Department of Biology, University of Crete, Heraklion, Crete, Greece.
authors.forename: Kriton
authors.initials: K
authors.lastname: Kalantidis
authors.affiliation: 1 Instituto de Biología Molecular y Celular de Plantas (Consejo Superior de Investigaciones Científicas-Universidad Politécnica de Valencia), 46022 Valencia, Spain; and.
authors.forename: José-Antonio
authors.initials: JA
authors.lastname: Daròs
mesh_terms: D015536:Down-Regulation; D017343:Genes, Plant; D005819:Genetic Markers; D009068:Movement; D010935:Plant Diseases / Q000821:virology; D010940:Plant Proteins / Q000378:metabolism*; D017800:Potyvirus / Q000502:physiology*; D014026:Nicotiana / Q000235:genetics / Q000382:microbiology / Q000821:virology*
chemical_list: D005819:Genetic Markers; D010940:Plant Proteins