title: An adenylyl cyclase with a phosphodiesterase domain in basal plants with a motile sperm system.
abstract: Adenylyl cyclase (AC), which produces the signalling molecule cAMP, has numerous important cellular functions in diverse organisms from prokaryotes to eukaryotes. Here we report the identification and characterization of an AC gene from the liverwort Marchantia polymorpha. The encoded protein has both a C-terminal AC catalytic domain similar to those of class III ACs and an N-terminal cyclic nucleotide phosphodiesterase (PDE) domain that degrades cyclic nucleotides, thus we designated the gene MpCAPE (COMBINED AC with PDE). Biochemical analyses of recombinant proteins showed that MpCAPE has both AC and PDE activities. In MpCAPE-promoter-GUS lines, GUS activity was specifically detected in the male sexual organ, the antheridium, suggesting MpCAPE and thus cAMP signalling may be involved in the male reproductive process. CAPE orthologues are distributed only in basal land plants and charophytes that use motile sperm as the male gamete. CAPE is a subclass of class III AC and may be important in male organ and cell development in basal plants.
authors.affiliation: Graduate School of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.|College of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kasahara
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Suetsugu
authors.affiliation: Graduate School of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Urano
authors.affiliation: College of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Yamamoto
authors.affiliation: College of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
authors.forename: Mikiya
authors.initials: M
authors.lastname: Ohmori
authors.affiliation: College of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Takada
authors.affiliation: Graduate School of Medical and Dental Sciences, Niigata University, Niigata 951-8510, Japan.
authors.forename: Shujiro
authors.initials: S
authors.lastname: Okuda
authors.affiliation: Advanced Science Research Center, Kanazawa University, Ishikawa 920-0934, Japan.
authors.forename: Tomoaki
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Department of Biology, Graduate School of Science, Kobe University, Hyogo 657-8501, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Sakayama
authors.affiliation: Graduate School of Biostudies, Kyoto University, Kyoto 606-8502, Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Kohchi
authors.affiliation: Graduate School of Life Sciences, Ritsumeikan University, Shiga 525-8577, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Takahashi
mesh_terms: D000262:Adenylyl Cyclases / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D000242:Cyclic AMP / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D047008:Marchantia / Q000201:enzymology*; D010727:Phosphoric Diester Hydrolases / Q000737:chemistry*; D010802:Phylogeny; D010940:Plant Proteins / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified / Q000378:metabolism; D000072417:Protein Domains / Q000235:genetics; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000235:genetics / Q000302:isolation & purification; D016415:Sequence Alignment; D015398:Signal Transduction
chemical_list: D010940:Plant Proteins; D011993:Recombinant Fusion Proteins; D000242:Cyclic AMP; D010727:Phosphoric Diester Hydrolases; D000262:Adenylyl Cyclases