title: [Oestrogen inhibits invasion and metastasis of hepatocellular carcinoma MHCC97H cells by regulating the activity of AKT signaling pathway].
abstract: OBJECTIVE
  To explore the inhibitory effect of estrogen against metastasis of human hepatocellular carcinoma MHCC97H cells and explore the molecular mechanism.
  
  
  METHODS
  The inhibitory effect of estrogen on the migration and invasion of MHCC97H cells was evaluated with wound healing assay and Transwell assay. Western blotting was used for investigating the expression of MMP-2, MMP-9, AKT and p-AKT in the cells treated with estrogen.
  
  
  RESULTS
  Estrogen treatment significantly inhibited the migration and invasion of MHCC97H cells in a dose-dependent manner. Estrogen significantly down-regulated the protein expressions of MMP-2 and MMP-9 and lowered the phosphorylation level of AKT.
  
  
  CONCLUSIONS
  The anti-metastatic effect of estrogen involves inhibition of MMP-2 and MMP-9 in MHCC97H cells probably by regulating AKT signal pathway.
authors.affiliation: Department of Infectious Diseases, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China. E-mail: lbdtcyty@163.com.
authors.forename: Chang-Yin
authors.initials: CY
authors.lastname: Tian
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.forename: Wen-Xue
authors.initials: WX
authors.lastname: Zhao
authors.forename: Shuang-Suo
authors.initials: SS
authors.lastname: Dang
authors.forename: Yao-Feng
authors.initials: YF
authors.lastname: Jin
authors.forename: Fan-Pu
authors.initials: FP
authors.lastname: Ji
mesh_terms: D006528:Carcinoma, Hepatocellular; D045744:Cell Line, Tumor; D002465:Cell Movement; D015536:Down-Regulation; D004967:Estrogens / Q000494:pharmacology*; D006801:Humans; D008113:Liver Neoplasms; D020778:Matrix Metalloproteinase 2 / Q000378:metabolism; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism; D009361:Neoplasm Invasiveness; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D004967:Estrogens; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9