title: Microfluidic Assembly of Monodisperse Vesosomes as Artificial Cell Models.
abstract: Vesosomes are nested liposomal structures with high potential as advanced drug delivery vehicles, bioreactors and artificial cells. However, to date no method has been reported to prepare monodisperse vesosomes of controlled size. Here we report on a multistep microfluidic strategy for hierarchically assembling uniform vesosomes from dewetting of double emulsion templates. The control afforded by our method is illustrated by the formation of concentric, pericentric and multicompartment liposomes. The microfluidic route to vesosomes offers an exceptional platform to build artificial cells as exemplified by the in vitro transcription in "nucleus" liposomes and the mimicry of the architecture of eukaryotic cells. Finally, we show the transport of small molecules across the nucleic envelope via insertion of nanopores into the bilayers.
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Nan-Nan
authors.identifier: 0000-0001-7183-7973
authors.initials: NN
authors.lastname: Deng
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Maaruthy
authors.initials: M
authors.lastname: Yelleswarapu
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Lifei
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Wilhelm T S
authors.identifier: 0000-0003-4222-5411
authors.initials: WT
authors.lastname: Huck
mesh_terms: D058045:Artificial Cells / Q000737:chemistry*; D016503:Drug Delivery Systems; D008081:Liposomes / Q000737:chemistry*; D044085:Microfluidics; D008954:Models, Biological; D022022:Nuclear Pore / Q000737:chemistry
chemical_list: D008081:Liposomes