title: Injection of Anti-proBDNF in Anterior Cingulate Cortex (ACC) Reverses Chronic Stress-Induced Adverse Mood Behaviors in Mice.
abstract: Foraging behavior is a species-specific behavior which is considered to involve the decision making and higher cognitive functions. We previously established a novel method to detect the foraging behavior in chronic unpredictable mild stress (CUMS)-induced depression mice, in which the food foraging activity of mice was significantly reduced. Furthermore, it is generally assumed that the bilateral anterior cingulate cortex (ACC) is related to foraging activity in rat. Brain-derived neurotrophic factor (BDNF) is widely expressed in many regions of the brain and is down-regulated in depressive patients. However, the relationship between the precursor of brain-derived neurotrophic factor (proBDNF) and depression has not been fully elucidated. The results showed that CUMS in mice induced anxiety- and depression-like behaviors and significant reduction in BDNF messenger RNA (mRNA) in the brain. In this study, we evaluated the effect of anti-BDNF and anti-proBDNF in the ACC on the CUMS-induced depression mice. In contrast to the normal IgG group (normal IgG microinjection into the ACC), bilateral ACC treatment with anti-proBDNF microinjection not only reversed depressive activity but also significantly increased the amount of foraged food and BDNF mRNA in the brain. There was no significant alteration in the group of anti-BDNF microinjection into the ACC. Our data indicate that the proBDNF signaling pathway might down-regulate the foraging activity in CUMS rodents and be involved in the depression.
authors.affiliation: Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, People's Republic of China. tjwqycr@163.com.|Department of Pathology, Second Hospital of Tianjin Medical University, Tianjin, People's Republic of China. tjwqycr@163.com.
authors.forename: C R
authors.initials: CR
authors.lastname: Yang
authors.affiliation: Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, People's Republic of China.|Department of Psychiatry, Liupanshui Third People's Hospital, Liupanshui, Guizhou, People's Republic of China.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Bai
authors.affiliation: Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, People's Republic of China.|School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
authors.forename: C S
authors.initials: CS
authors.lastname: Ruan
authors.affiliation: School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia.
authors.forename: F H
authors.initials: FH
authors.lastname: Zhou
authors.affiliation: Department of Neurobiology, Xiangya Medical College, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: F
authors.initials: F
authors.lastname: Li
authors.affiliation: Department of Neurobiology, Xiangya Medical College, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: C Q
authors.initials: CQ
authors.lastname: Li
authors.affiliation: Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, People's Republic of China. xin-fu.zhou@unisa.edu.au.|School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, SA, 5000, Australia. xin-fu.zhou@unisa.edu.au.
authors.forename: X F
authors.initials: XF
authors.lastname: Zhou
mesh_terms: D000818:Animals; D000906:Antibodies / Q000008:administration & dosage / Q000627:therapeutic use*; D001522:Behavior, Animal / Q000187:drug effects / Q000502:physiology; D019208:Brain-Derived Neurotrophic Factor / Q000037:antagonists & inhibitors / Q000378:metabolism / Q000502:physiology*; D002540:Cerebral Cortex / Q000378:metabolism; D003863:Depression / Q000523:psychology*; D004195:Disease Models, Animal; D005106:Exploratory Behavior / Q000187:drug effects / Q000502:physiology*; D005247:Feeding Behavior / Q000187:drug effects; D006179:Gyrus Cinguli / Q000378:metabolism / Q000502:physiology*; D006624:Hippocampus / Q000378:metabolism; D007074:Immunoglobulin G / Q000008:administration & dosage / Q000627:therapeutic use; D008297:Male; D051379:Mice; D008845:Microinjections; D011498:Protein Precursors / Q000037:antagonists & inhibitors / Q000502:physiology*; D013315:Stress, Psychological / Q000523:psychology*
chemical_list: D000906:Antibodies; D019208:Brain-Derived Neurotrophic Factor; D007074:Immunoglobulin G; D011498:Protein Precursors; C426674:brain-derived neurotrophic factor precursor
keywords: Anterior cingulate cortex (ACC); Anti-proBDNF; Anxiety-like behavior; Depression-like behavior; Foraging behavior; Mice