title: Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging.
abstract: Bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (EDF), which makes it possible to observe fast events volumetrically. However, the depth information is lost due to integration of fluorescence signals along the propagation direction. We describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3D) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. The depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.
authors.forename: Yanlong
authors.initials: Y
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Dan
authors.affiliation: State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an, China.
authors.forename: Runze
authors.initials: R
authors.lastname: Li
authors.affiliation: Department of Radiology, Medical University of South Carolina, Charleston, South Carolina, United States of America.
authors.forename: Mark Van
authors.initials: MV
authors.lastname: Horn
authors.affiliation: Department of Bioengineering and The Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Xun
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Bioengineering and The Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, Clemson, South Carolina, United States of America.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Bioengineering and The Center for Optical Materials Science and Engineering Technologies (COMSET), Clemson University, Clemson, South Carolina, United States of America.|Department of Regenerative Medicine and Cell Biology, Medical University of South Carolina, Charleston, South Carolina, United States of America.
authors.forename: Tong
authors.initials: T
authors.lastname: Ye
mesh_terms: D000465:Algorithms; D006801:Humans; D021621:Imaging, Three-Dimensional / Q000379:methods*; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods