title: Electroacupuncture Restores 5-HT System Deficit in Chronic Mild Stress-Induced Depressed Rats.
abstract: Objective.The current study is designed to investigate the antidepressant efficacy of electroacupuncture (EA) treatment by evaluating its effect on the synthesis, metabolism, reuptake, and receptors of 5-hydroxytryptamine (5-HT), so as to clarify the molecular mechanisms of EA for antidepression.Materials and Methods.Solitary combined with the chronic unpredictable mild stress (CUMS) was used to establish the rat model with depression. The depressed rats were supplied with EA treatment for 4 weeks, and the behavior change and the following indices including 5-HT, 5-hydroxyindoleacetic acid (5-HIAA), monoamine oxidase A (MAO-A), tryptophan hydroxylase (TPH), 5-HT transporter (SERT), 5-HT1A, and 5-HT2A in hippocampus and prefrontal cortex were examined.Results.EA treatment significantly improved the behavior of rats and increased 5-HT level in hippocampus of depressed rats. Similarly, EA treatment could significantly increase protein and mRNA expression of TPH and 5-HT1A during 5-HT synthesis process in hippocampus of depressed rats. However, EA treatment had no effect on the activity of MAO-A and the expression of SERT protein and mRNA.Conclusion.Antidepressant efficacy of EA treatment can be accomplished through enhancing 5-HT synthesis, upregulating 5-HT1A level, and improving 5-HT content in brain and synaptic gaps.
authors.affiliation: Department of Health Care of South Building, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Dongmei
authors.identifier: 0000-0002-2650-5854
authors.initials: D
authors.lastname: Duan
authors.affiliation: Acupuncture College of Beijing University of Chinese Medicine, Beijing 100029, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Tu
authors.affiliation: Beijing University of Chinese Medicine, Beijing 10029, China.
authors.forename: Xiuyan
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Clinical Pharmacology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu