title: Differential Protein Adsorption and Cellular Uptake of Silica Nanoparticles Based on Size and Porosity.
abstract: Slight alterations in nanoparticles' surface properties can significantly influence the corona composition which may alter their interaction with the biological milieu. Size and porosity of silica nanoparticles (SNPs) are known to be predominant factors influencing their dose-dependent toxicity. Little is known however about the extent and type of protein adsorption on SNPs as a function of physicochemical properties and the role this might play on mechanisms of cellular uptake and toxicity. In this work we investigated the influence of size and porosity of SNPs on protein adsorption, cellular uptake, and toxicity in RAW 264.7 macrophages. Toxicity of the SNPs was found to be concentration dependent, and the formation of the protein corona mitigated toxicity for all particles. Detailed analysis of the amount of proteins recovered from each nanoparticle revealed similarities in the protein adsorption profile as a function of size and porosity. The mechanism of uptake was highly dependent on size rather than porosity or the adsorbed proteins.
authors.affiliation: Utah Center for Nanomedicine, Nano Institute of Utah, §Department of Pharmaceutics and Pharmaceutical Chemistry, and ∥Department of Bioengineering, University of Utah , Salt Lake City, Utah 84112, United States.
authors.forename: Jiban
authors.initials: J
authors.lastname: Saikia
authors.affiliation: Utah Center for Nanomedicine, Nano Institute of Utah, §Department of Pharmaceutics and Pharmaceutical Chemistry, and ∥Department of Bioengineering, University of Utah , Salt Lake City, Utah 84112, United States.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Yazdimamaghani
authors.affiliation: Utah Center for Nanomedicine, Nano Institute of Utah, §Department of Pharmaceutics and Pharmaceutical Chemistry, and ∥Department of Bioengineering, University of Utah , Salt Lake City, Utah 84112, United States.
authors.forename: Seyyed Pouya
authors.initials: SP
authors.lastname: Hadipour Moghaddam
authors.affiliation: Utah Center for Nanomedicine, Nano Institute of Utah, §Department of Pharmaceutics and Pharmaceutical Chemistry, and ∥Department of Bioengineering, University of Utah , Salt Lake City, Utah 84112, United States.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Ghandehari
keywords: cellular uptake; cytotoxicity; protein adsorption; protein corona; silica nanoparticles