title: Chitosan-Based Matrices Prepared by Gamma Irradiation for Tissue Regeneration: Structural Properties vs. Preparation Method.
abstract: In the last decade, new generations of biopolymer-based materials have attracted attention, aiming its application as scaffolds for tissue engineering. These engineered three-dimensional scaffolds are designed to improve or replace damaged, missing, or otherwise compromised tissues or organs. Despite the number of promising methods that can be used to generate 3D cell-instructive matrices, the innovative nature of the present work relies on the application of ionizing radiation technology to form and modify surfaces and matrices with advantage over more conventional technologies (room temperature reaction, absence of harmful initiators or solvents, high penetration through the bulk materials, etc.), and the possibility of preparation and sterilization in one single step. The current chapter summarizes the work done by the authors in the gamma radiation processing of biocompatible and biodegradable chitosan-based matrices for skin regeneration. Particular attention is given to the correlation between the different preparation conditions and the final polymeric matrices' properties. We therefore expect to demonstrate that instructive matrices produced and improved by radiation technology bring to the field of skin regenerative medicine a supplemental advantage over more conservative techniques.
authors.affiliation: Centro de Ciências e Tecnologias Nucleares (C2TN), Instituto Superior Técnico, Universidade de Lisboa, E.N. ao km 139.7, Bobadela, 2695-066, Loures, Portugal. casimiro@ctn.tecnico.ulisboa.pt.
authors.forename: Maria Helena
authors.identifier: http://orcid.org/0000-0002-0352-5721
authors.initials: MH
authors.lastname: Casimiro
authors.affiliation: Centro de Ciências e Tecnologias Nucleares (C2TN), Instituto Superior Técnico, Universidade de Lisboa, E.N. ao km 139.7, Bobadela, 2695-066, Loures, Portugal.
authors.forename: Joana J H
authors.initials: JJH
authors.lastname: Lancastre
authors.affiliation: Centro de Ciências e Tecnologias Nucleares (C2TN), Instituto Superior Técnico, Universidade de Lisboa, E.N. ao km 139.7, Bobadela, 2695-066, Loures, Portugal.
authors.forename: Alexandra P
authors.initials: AP
authors.lastname: Rodrigues
authors.affiliation: Departamento de Biologia Animal, Faculdade de Ciências, Centro de Ecologia, Evolução e Alterações Ambientais (cE3c), Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal.
authors.forename: Susana R
authors.initials: SR
authors.lastname: Gomes
authors.affiliation: Departamento de Biologia Animal, Faculdade de Ciências, Centro de Ecologia, Evolução e Alterações Ambientais (cE3c), Universidade de Lisboa, Campo Grande, 1749-016, Lisbon, Portugal.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Rodrigues
authors.affiliation: Centro de Ciências e Tecnologias Nucleares (C2TN), Instituto Superior Técnico, Universidade de Lisboa, E.N. ao km 139.7, Bobadela, 2695-066, Loures, Portugal. ferrreira@ctn.tecnico.ulisboa.pt.
authors.forename: Luís M
authors.initials: LM
authors.lastname: Ferreira
keywords: Chitosan; Gamma irradiation; Porous scaffolds; Skin substitute; Tissue engineering