title: Study of Fluorescent Imaging of Se (IV) in Living Cells Using a Turn-on Fluorescent Probe Based on a Rhodamine Spirolactame Derivative.
abstract: A highly selective fluorescent probe 2-(2-(2-aminoethylamino)ethyl)-3',6'-bis(ethylamino)-2',7'-dimethylspiro[isoindoline-1,9'-xanthen]-3-one (ABDO) for Se (IV) had been synthesized in our earlier report. In this study, this fluorescent sensor is applied on analysis fluorescent imaging of Se (IV) in Hela cells. The experiment conditions, such as the MTT assay, different concentration of saline, incubated time of Hela cells with ABDO and Se (IV), and intracellular action position of Se (IV), are investigated. Through a series of experiments, the fluorescent image of Se (IV) in Hela cells can be observed when the cells cultured by 2 μM ABDO and 2 μM Se (IV) for 210 min. And the intracellular action position of Se (IV) is verified after the co-localization experiments are done. It is mitochondria. These experimental results show that ABDO will be an eagerly anticipated sensor for fluorescent imaging analysis of selenium ion in living cells. Besides, we also can use the complexes of ABDO-Se to observe morphology and distribution of mitochondria in cells like JG-B.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Guan
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Mi
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fei
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Shan
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Yanfu
authors.initials: Y
authors.lastname: Huan
authors.affiliation: Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun, 130012, China. lvsw@jlu.edu.cn.
authors.forename: Shaowu
authors.initials: S
authors.lastname: Lv
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun, 130012, China. fenggd@jlu.edu.cn.
authors.forename: Guodong
authors.initials: G
authors.lastname: Feng
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence / Q000379:methods*; D008958:Models, Molecular; D015394:Molecular Structure; D012235:Rhodamines / Q000737:chemistry*; D018036:Selenium Compounds / Q000032:analysis*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; D018036:Selenium Compounds
keywords: Fluorescent probe; Hela cells; Live cell imaging; Mitochondria; Rhodamine; Selenium