title: Structural elucidation and immunostimulatory activity of polysaccharide isolated by subcritical water extraction from Cordyceps militaris.
abstract: Water-soluble polysaccharides were obtained from Cordyceps militaris (C. militaris) (CMP) by subcritical water extraction (SWE). Two polysaccharides fractions, CMP-W1 and CMP-S1, were isolated from CMP using DEAE-52 cellulose and Sephadex G-150 column chromatography. The structural characteristics of CMP-W1 and CMP-S1 were investigated. The results showed that the molecular weight of CMP-W1 and CMP-S1 are 3.66×105Da and 4.60×105Da, respectively, and both of them were heteropolysaccharides composed of d-mannose, d-glucose, d-galactose with the molar ratios of 2.84:1:1.29 and 2.05:1:1.09, respectively. FT-IR spectra analysis suggested that CMP-W1 and CMP-S1 belonged to pyranose form sugar and protein free. For immunostimulatory activity assay in vitro, CMP-W1 and CMP-S1 significantly promoted lymphatic spleen cell proliferation of mice. Therefore, the polysaccharides obtained from C. militaris by SWE can be used as potential natural immunostimulant in functional foods or medicine.
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Luo
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: dyq101@ujs.edu.cn.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: Duan
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Wenya
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: Zhanghh@mail.ujs.edu.cn.
authors.forename: Haihui
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Changzheng
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Jixian
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000276:Adjuvants, Immunologic / Q000302:isolation & purification / Q000494:pharmacology; D000818:Animals; D002478:Cells, Cultured; D005591:Chemical Fractionation; D032661:Cordyceps / Q000737:chemistry*; D008214:Lymphocytes / Q000187:drug effects; D051379:Mice; D009005:Monosaccharides; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D017550:Spectroscopy, Fourier Transform Infrared; D013154:Spleen / Q000166:cytology; D014867:Water
chemical_list: D000276:Adjuvants, Immunologic; D009005:Monosaccharides; D011134:Polysaccharides; D014867:Water
keywords: Characterization; Cordyceps militaris; Immunostimulatory activity; Purification; Subcritical water extraction (SWE)