title: Prognostic value of candidate microRNAs in gastric cancer: A validation study.
abstract: BACKGROUND
  Studies have reported the prognostic value of dysregulated microRNAs (miRNAs) in gastric cancer (GC). However, the results demonstrated so far are inconsistent.
  
  
  OBJECTIVE
  To better understand the miRNAs with prognostic relevance.
  
  
  METHODS
  Evaluable miRNAs were selected based on our selection criteria and further analyzed in formalin-fixed paraffin-embedded (FFPE) tissue samples of 169 GC patients using quantitative real-time polymerase chain reaction (qRT-PCR).
  
  
  RESULTS
  A total of 19 miRNAs were selected as candidate miRNAs. Among those miRNAs identified, high expression of miR-21-5p was related to poor overall survival (OS) and disease free survival (DFS) and was identified as an independent prognostic factor. Cases with high level of miR-200c-3p showed poor DFS. Subgroup analysis revealed that high expression of miR-21-5p and miR-222-3p was associated with poor OS and DFS in GC patients not received adjuvant chemotherapy. In male patients, high expression level of miR-21-5p was related to poor OS and DFS.
  
  
  CONCLUSIONS
  The present study confirmed that elevated level of miR-21-5p could serve as an independent predictor for poor OS and DFS of GC patients. Moreover, miR-200c-3p, miR-222-3p might also play important roles in the prognosis of GC patients. Further studies are warranted to validate our findings and identify the functions and mechanisms of these miRNAs.
authors.forename: Lan
authors.initials: L
authors.lastname: Zhang
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
authors.forename: Huo
authors.initials: H
authors.lastname: Zhang
authors.forename: Mingxia
authors.initials: M
authors.lastname: Zhu
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor; D017024:Chemotherapy, Adjuvant; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D060787:Neoplasm Grading; D009362:Neoplasm Metastasis; D009367:Neoplasm Staging; D011379:Prognosis; D013274:Stomach Neoplasms / Q000175:diagnosis / Q000188:drug therapy / Q000235:genetics* / Q000401:mortality*
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: MiRNA; gastric cancer; prognosis