title: Usage of Murine T-cell Hybridoma Cells as Responder Cells Reveals Interference ofHelicobacter Pyloriwith Human Dendritic Cell-mediated Antigen Presentation.
abstract: Direct effects ofHelicobacter pylori (H. pylori)on human CD4+T-cells hamper disentangling a possible bacterial-mediated interference with major histocompatibility complex class II (MHC-II)-dependent antigen presentation to these cells. To overcome this limitation, we employed a previously described assay, which enables assessing human antigen-processing cell function by using murine T-cell hybridoma cells restricted by human leukocyte antigen (HLA) alleles. HLA-DR1+monocyte-derived dendritic cells were exposed toH. pyloriand pulsed with the antigen 85B fromMycobacterium tuberculosis (M. tuberculosis). Interleukin-2 (IL-2) secretion by AG85Baa97-112-specific hybridoma cells was then evaluated as an integral reporter of cognate antigen presentation. This methodology enabled revealing of interference ofH. pyloriwith the antigen-presenting capacity of human dendritic cells.
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Infection Biology , Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Michael
authors.initials: M
authors.lastname: Fehlings
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Infection Biology , Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Lea
authors.initials: L
authors.lastname: Drobbe
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Infection Biology , Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Macarena
authors.initials: M
authors.lastname: Beigier-Bompadre
authors.affiliation: Institute for Microbiology and Hygiene, Charité - Universitätsmedizin Berlin , 12203 Berlin, Germany.
authors.forename: Pablo Renner
authors.initials: PR
authors.lastname: Viveros
authors.affiliation: Charité - Universitätsmedizin Berlin, Campus Benjamin Franklin , Hindenburgdamm 30, 12200 Berlin, Germany.
authors.forename: Verena
authors.initials: V
authors.lastname: Moos
authors.affiliation: Charité - Universitätsmedizin Berlin, Campus Benjamin Franklin , Hindenburgdamm 30, 12200 Berlin, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Schneider
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Infection Biology , Charitéplatz 1, 10117 Berlin, Germany.
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Meyer
authors.affiliation: Mycotic and Parasitic Agents and Mycobacteria, Department of Infectious Diseases, Robert Koch-Institute , Berlin, Germany.
authors.forename: Toni
authors.initials: T
authors.lastname: Aebischer
authors.affiliation: Institute for Microbiology and Hygiene, Charité - Universitätsmedizin Berlin , 12203 Berlin, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Ignatius
keywords: Helicobacter pylori; T-cells; antigen presentation; dendritic cells; hybridoma cells