title: Human Bocavirus in Korean Children with Gastroenteritis and Respiratory Tract Infections.
abstract: Human bocaviruses (HBoVs) are suggested to be etiologic agents of childhood respiratory and gastrointestinal infections. There are four main recognized genotypes of HBoVs (HBoV1-4); the HBoV-1 genotype is considered to be the primary etiologic agent in respiratory infections, whereas the HBoV2-4 genotypes have been mainly associated with gastrointestinal infections. The aim of the present study was to determine the distribution of HBoV genotypes in children with respiratory or gastrointestinal infections in a hospital in Korea. A total of 662 nasopharyngeal swabs (NPSs) and 155 fecal specimens were collected from children aged 5 years or less. Polymerase chain reaction (PCR) was conducted to detect theNS1HBoV gene. TheVP1gene of HBoV was further amplified in samples that were positive for theNS1gene. The PCR products ofVP1gene amplification were genotyped by sequence analysis. HBoV was detected in 69 (14.5%) of 662 NPSs and in 10 (6.5%) of 155 fecal specimens. Thirty-three isolates from NPSs and five isolates from fecal specimens were genotyped, and all 38 sequenced isolates were identified as the HBoV-1 genotype. HBoV-1 is the most prevalent genotype in children with respiratory or gastrointestinal HBoV infections in a hospital in Korea.
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: Eun Jin
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: Han-Sung
authors.identifier: 0000-0002-5481-5390
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: Hyun Soo
authors.identifier: 0000-0002-7026-6715
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: Jae-Seok
authors.initials: JS
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: Wonkeun
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: MiYoung
authors.initials: M
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: Young Kyung
authors.initials: YK
authors.lastname: Lee
authors.affiliation: Department of Laboratory Medicine, Hallym University College of Medicine, Gyeonggi-do, Republic of Korea.
authors.forename: Hee Jung
authors.identifier: 0000-0003-1249-6181
authors.initials: HJ
authors.lastname: Kang
mesh_terms: D000067576:Child Health / Q000706:statistics & numerical data; D002675:Child, Preschool; D015897:Comorbidity; D005260:Female; D005759:Gastroenteritis / Q000453:epidemiology* / Q000821:virology*; D056661:Human bocavirus / Q000302:isolation & purification*; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D010322:Parvoviridae Infections / Q000453:epidemiology* / Q000821:virology*; D015995:Prevalence; D056910:Republic of Korea / Q000453:epidemiology; D012141:Respiratory Tract Infections / Q000453:epidemiology*; D012307:Risk Factors