title: Tissue Extract Fractions from Starfish Undergoing Regeneration Promote Wound Healing and Lower Jaw Blastema Regeneration of Zebrafish.
abstract: Natural bioactive materials provide an excellent pool of molecules for regenerative therapy. In the present study, we amputate portions of the arms of Archaster typicus starfish, extract and separate the active biomaterials, and compare the effects of each fraction on in vitro wound healing and in vivo lower jaw regeneration of zebrafish. Compared with crude extract, normal hexane fractions (NHFs) have a remarkable effect on cellular proliferation and collective migration, and exhibit fibroblast-like morphology, while methanol-water fractions (MWFs) increase cell size, cell-cell adhesion, and cell death. Relative to moderate mitochondrialand lysosomal aggregation in NHFs-cultured cells, MWFs-cultured cells contain more and bigger lysosomal accumulations and clump detachment. The in vivo zebrafish lower jaw regeneration model reveals that NHFs enhance blastema formation and vasculogenesis, while MWFs inhibit fibrogenesis and induce cellular transformation. Gene expression analyses indicate that NHFs and MWFs separately activate blastema-characteristic genes as well as those genes-related to autophagy, proteasome, and apoptosis either during cell scratch healing or ganciclovir-induced apoptosis. Our results suggest that bioactive compounds from NHFs and MWFs could induce blastema formation and remodeling, respectively, and prevent tissue overgrowth.
authors.affiliation: Institute for Marine Biosystem and Neurosciences, Department of Biology, College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Yancen
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Nagarajan
authors.initials: N
authors.lastname: Prithiviraj
authors.affiliation: College of Food and Science, Shanghai Ocean University, Shanghai 201306, China.|Key Laboratory of Marine Biopharmaceuticals, Renji Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Gan
authors.affiliation: Stephenson Cancer Center and Department of Physiology, The University of Oklahoma Health Sciences Center, OK 73104, USA.
authors.forename: Xin A
authors.initials: XA
authors.lastname: Zhang
authors.affiliation: Institute for Marine Biosystem and Neurosciences, Department of Biology, College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China.|Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China.
authors.forename: Jizhou
authors.initials: J
authors.lastname: Yan
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects; D001672:Biocompatible Materials / Q000494:pharmacology; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D002470:Cell Survival / Q000187:drug effects; D004817:Epidermis / Q000187:drug effects / Q000473:pathology; D005453:Fluorescence; D005786:Gene Expression Regulation / Q000187:drug effects; D007568:Jaw / Q000473:pathology*; D008247:Lysosomes / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism; D008954:Models, Biological; D010641:Phenotype; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D012038:Regeneration / Q000187:drug effects*; D013215:Starfish / Q000033:anatomy & histology / Q000737:chemistry*; D014020:Tissue Extracts / Q000494:pharmacology*; D014945:Wound Healing; D015027:Zebrafish
chemical_list: D001672:Biocompatible Materials; D014020:Tissue Extracts; D046988:Proteasome Endopeptidase Complex