title: Deregulation of miR-183 promotes melanoma development via lncRNA MALAT1 regulation and ITGB1 signal activation.
abstract: Dysregulation of miR-183 has been recently elucidated in several carcinomas. However, the expression patterns and mechanisms of miR-183 involved in malignant melanoma remain unidentified. Here, we found down-regulation of miR-183 in melanoma tissues and cells. Decreased level of miR-183 was relevant to poor overall survival, while miR-183 up-regulation resulted in a marked suppression of cell growth in vitro and in vivo. We further found that the expression and function of miR-183 were suppressed by MALAT1. Integrin β1 (ITGB1) was then speculated and confirmed as a direct target of miR-183. We also illustrated that MALAT1 may function as a sponge competitive endogenous RNA (ceRNA) for miR-183, and thus regulate the molecular expression of ITGB1. Collectively, we found a new signaling pathway promoting melanoma development by MALAT1-miR-183-ITGB1 axis, which may be clinically valuable as new targets for malignant melanoma therapy.
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Guangjun
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Anesthesiology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Fan
authors.affiliation: Department of Burn and Plastic Surgery, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, China.
authors.forename: Weixi
authors.initials: W
authors.lastname: Yang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D004195:Disease Models, Animal; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D019012:Integrin beta1 / Q000235:genetics / Q000378:metabolism*; D008545:Melanoma / Q000235:genetics* / Q000378:metabolism* / Q000401:mortality; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D008954:Models, Biological; D011379:Prognosis; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics*; D015398:Signal Transduction; D023041:Xenograft Model Antitumor Assays
chemical_list: D019012:Integrin beta1; C557041:MALAT1 long non-coding RNA, human; C533449:MIRN183 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding
keywords: ITGB1; MALAT1; competitive endogenous RNA; melanoma; miR-183