title: Arabinoxylan hydrolyzates as immunomodulators in Caco-2 and HT-29 colon cancer cell lines.
abstract: The use of plant derived polysaccharides as health promoters has gained immense interest in the past few years. Arabinoxylan (AX) is the predominant non-starch polysaccharide in cereals and grasses including wheat. The current research aimed to investigate the structure-function relationship of arabinoxylan hydrolyzates (AXH), obtained by the enzymatic hydrolysis of AX using xylanase and arabinofuranosidase as immunomodulators in two colon cancer cell lines: Caco-2 and HT-29. Fine structural details had a strong correlation with the immunological properties of the wheat AXH. As a general trend, as the presence of arabinose substitution increased in the AXH, the production of proinflammatory cytokines, IL-8 and TNF-α, decreased in both cell lines. Thus, AXH with a higher degree of arabinose substitution might be better adept in lowering inflammation in colon cancer cells.
authors.affiliation: North Dakota State University, Department of Plant Sciences, P.O. Box 6050, Department 7670, Fargo, ND 58105, USA. senay.simsek@ndsu.edu.
authors.forename: Mihiri
authors.initials: M
authors.lastname: Mendis
authors.affiliation: North Dakota State University, Department of Pharmaceutical Sciences, College of Health Professions, Fargo, ND, USA.
authors.forename: Estelle
authors.initials: E
authors.lastname: Leclerc
authors.affiliation: North Dakota State University, Department of Plant Sciences, P.O. Box 6050, Department 7670, Fargo, ND 58105, USA. senay.simsek@ndsu.edu.
authors.forename: Senay
authors.initials: S
authors.lastname: Simsek
mesh_terms: D018938:Caco-2 Cells; D003110:Colonic Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000276:immunology*; D043364:Endo-1,4-beta Xylanases / Q000737:chemistry; D006026:Glycoside Hydrolases / Q000737:chemistry; D019073:HT29 Cells; D006801:Humans; D006868:Hydrolysis; D007155:Immunologic Factors / Q000737:chemistry* / Q000494:pharmacology*; D016209:Interleukin-8 / Q000235:genetics / Q000276:immunology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000276:immunology; D014990:Xylans / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D007155:Immunologic Factors; D016209:Interleukin-8; D014409:Tumor Necrosis Factor-alpha; D014990:Xylans; C085118:arabinoxylan; D006026:Glycoside Hydrolases; D043364:Endo-1,4-beta Xylanases