title: Ultrasound as an alternative technology to extract carotenoids and lipids from Heterochlorella luteoviridis.
abstract: The present work evaluated the use of ultrasound as a pre-treatment to lipid and carotenoid extraction from the microalgae Heterochlorella luteoviridis. The pre-treatment was performed in the presence of ethanol (25%, v/v) with the ultrasound intensity varying from 0 to 100% (435kJkg-1). After the pre-treatment, a diffusive step was performed in order to evaluate different ethanol concentrations (50-75%, v/v). The results regarding carotenoid extraction showed that there is an optimal extraction region: 40-80% of ultrasound intensity and 60-75% of ethanol concentration. The lipid extraction was not influenced by the ultrasound pre-treatment and increased with the increase of ethanol concentration.
authors.affiliation: Chemical Engineering Department, Federal University of Rio Grande do Sul (UFRGS), Engenheiro Luiz Englert Street, Porto Alegre, RS, 90040-040, Brazil. Electronic address: deborapj@enq.ufrgs.br.
authors.forename: Débora Pez
authors.initials: DP
authors.lastname: Jaeschke
authors.affiliation: Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Bento Gonçalves Avenue, 9500, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Rosane
authors.initials: R
authors.lastname: Rech
authors.affiliation: Chemical Engineering Department, Federal University of Rio Grande do Sul (UFRGS), Engenheiro Luiz Englert Street, Porto Alegre, RS, 90040-040, Brazil.
authors.forename: Ligia Damasceno Ferreira
authors.initials: LDF
authors.lastname: Marczak
authors.affiliation: Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Bento Gonçalves Avenue, 9500, Porto Alegre, RS, 91501-970, Brazil.
authors.forename: Giovana Domeneghini
authors.initials: GD
authors.lastname: Mercali
mesh_terms: D002338:Carotenoids / Q000302:isolation & purification*; D005591:Chemical Fractionation / Q000379:methods; D000460:Chlorophyta / Q000737:chemistry*; D000431:Ethanol / Q000737:chemistry; D008055:Lipids / Q000302:isolation & purification*; D058086:Microalgae / Q000737:chemistry; D014465:Ultrasonics / Q000379:methods*
chemical_list: D008055:Lipids; D002338:Carotenoids; D000431:Ethanol
keywords: Ethanol; Fatty acids; Microalgae; Pigments