title: Pegylated and amphiphilic Chitosan coated manganese ferrite nanoparticles for pH-sensitive delivery of methotrexate: Synthesis and characterization.
abstract: Magnetic nanoparticles (MNPs) are the major class of nanoparticles (NPs) with specific functional properties that make them good candidates for biomedical applications. Due to their response to the magnetic field, they can be used in targeted drug delivery systems. In current research, the MNPs were synthesized with the general formula of Fe1-xMnxFe2O4by the co-precipitation technique. First, the effect of the Fe2+ions in the system was investigated. Succinic anhydride was used as the first stabilizer to prepare surface for binding two types of polymer, including Polyethylene glycol (PEG) and palmitoylated Polyethylene glycol-grafted Chitosan (Cs-PEG-PA) were introduced as a polymeric shell. The composition, size, structure and magnetic properties of NPs were determined by the particle size analysis (PSA), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FTIR) and vibrating sample magnetometer (VSM). Determining the well-defined properties of MNPs, methotrexate (MTX), as a common anticancer drug, was encapsulated into the coated MNPs. The drug encapsulation efficiency was as high as 92.8% with the magnetization value of 19.7emu/g. The in-vitro release pattern was studied, showing only 6% of the drug release in pH=7.4 (as a model of the physiological environment) and 25% in pH=5.4 (as a model of the tumor tissue environment) after 72h. Based on these results, we may be able to introduce this specific system as a novel pH sensitive MNP system for MTX targeting to tumor tissues in cancer chemotherapy.
authors.affiliation: Department of materials Engineering, Institute of Mechanical Engineering, University of Tabriz, Tabriz 51666-16471, Iran.
authors.forename: Z
authors.initials: Z
authors.lastname: Karimi
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: S
authors.initials: S
authors.lastname: Abbasi
authors.affiliation: Electroceramics Group, Department of Materials Science and Engineering, Shiraz University of Technology, Shiraz, Iran. Electronic address: shokrollahi@sutech.ac.ir.
authors.forename: H
authors.initials: H
authors.lastname: Shokrollahi
authors.affiliation: Department of Pharmaceutics, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; Center for Nanotechnology in Drug Delivery, Shiraz University of Medical Sciences, Shiraz, Iran. Electronic address: ghyousefi@sums.ac.ir.
authors.forename: Gh
authors.initials: G
authors.lastname: Yousefi
authors.affiliation: Electroceramics Group, Department of Materials Science and Engineering, Shiraz University of Technology, Shiraz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Fahham
authors.affiliation: Materials Science and Engineering Department, Islamic Azad University Ahvaz Branch, Ahvaz, Iran.
authors.forename: L
authors.initials: L
authors.lastname: Karimi
authors.affiliation: Medicinal and Natural Products Chemistry Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: O
authors.initials: O
authors.lastname: Firuzi
mesh_terms: D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005290:Ferric Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D061986:MCF-7 Cells; D017895:Manganese Compounds / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008727:Methotrexate / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology
chemical_list: D020099:Coated Materials, Biocompatible; D003692:Delayed-Action Preparations; D005290:Ferric Compounds; D017895:Manganese Compounds; C551151:manganese ferrite; D011092:Polyethylene Glycols; D048271:Chitosan; C000595209:polyethylene glycol 1000; D008727:Methotrexate
keywords: Chitosan; Co-precipitation; Manganese magnetic nanoparticles; Methotrexate; PEG; pH- sensitive