title: Identification of miR-601 as a novel regulator in the development of pancreatic cancer.
abstract: Pancreatic cancer (PC) is one of the most aggressive malignancies, with a high mortality. Distant metastasis and recurrence are the main causes of PC-related deaths. MicroRNAs (miRNAs) have been reported in the serum or tumor tissue of cancer patients, including PC, which makes them potential biomarkers. The dysfunction of many miRNAs has been linked to PC occurrence and metastasis. In the current study, we found that miR-601 expression was significantly lower in PC samples, especially in metastatic compared to non-metastatic PC tissues. Gain-of-function and loss-of-function analysis showed that miR-601 suppressed PC cell proliferation and migration. One potential mechanism is that miR-601 inhibited the expression of Sirtuin 1 (SIRT1), which is a well-known regulator of PC development. We found that overexpression of SIRT1 could reverse the effect of miR-601 on PC cells. Our investigation therefore suggests that both miR-601 and SIRT1 are possible biomarkers for the early detection, and targets for the treatment of PC.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, No.296, Shizi Road, Suzhou, 215006, China.
authors.forename: Wenxi
authors.initials: W
authors.lastname: Cao
authors.affiliation: Department of Heptabiliary Surgery, Wuxi No.2 People's Hospital, No.68, Zhongshan Road, Wuxi, 214001, China.
authors.forename: Huihan
authors.initials: H
authors.lastname: Jin
authors.affiliation: Department of Heptabiliary Surgery, Wuxi No.2 People's Hospital, No.68, Zhongshan Road, Wuxi, 214001, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Heptabiliary Surgery, Wuxi No.2 People's Hospital, No.68, Zhongshan Road, Wuxi, 214001, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of General Surgery, The First Affiliated Hospital of Soochow University, No.296, Shizi Road, Suzhou, 215006, China. Electronic address: haixinqian2015@163.com.
authors.forename: Haixin
authors.initials: H
authors.lastname: Qian
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D009362:Neoplasm Metastasis; D009364:Neoplasm Recurrence, Local; D016376:Oligonucleotides, Antisense / Q000235:genetics; D010190:Pancreatic Neoplasms / Q000235:genetics / Q000378:metabolism*; D056564:Sirtuin 1 / Q000378:metabolism*; D014162:Transfection
chemical_list: C580741:MIRN601 microRNA, human; D035683:MicroRNAs; D016376:Oligonucleotides, Antisense; C447939:SIRT1 protein, human; D056564:Sirtuin 1
keywords: Pancreatic cancer; SIRT1; miR-601