title: Validating Intravascular Imaging with Serial Optical Coherence Tomography and Confocal Fluorescence Microscopy.
abstract: Atherosclerotic cardiovascular diseases are characterized by the formation of a plaque in the arterial wall. Intravascular ultrasound (IVUS) provides high-resolution images allowing delineation of atherosclerotic plaques. When combined with near infrared fluorescence (NIRF), the plaque can also be studied at a molecular level with a large variety of biomarkers. In this work, we present a system enabling automated volumetric histology imaging of excised aortas that can spatially correlate results with combined IVUS/NIRF imaging of lipid-rich atheroma in cholesterol-fed rabbits. Pullbacks in the rabbit aortas were performed with a dual modality IVUS/NIRF catheter developed by our group. Ex vivo three-dimensional (3D) histology was performed combining optical coherence tomography (OCT) and confocal fluorescence microscopy, providing high-resolution anatomical and molecular information, respectively, to validate in vivo findings. The microscope was combined with a serial slicer allowing for the imaging of the whole vessel automatically. Colocalization of in vivo and ex vivo results is demonstrated. Slices can then be recovered to be tested in conventional histology.
authors.affiliation: Département de Génie Électrique et Institut de Génie Biomédical, École Polytechnique de Montréal, Montreal, QC H3T 1J4, Canada. pierluctardif@gmail.com.|Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. pierluctardif@gmail.com.
authors.forename: Pier-Luc
authors.initials: PL
authors.lastname: Tardif
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. mariejeanne.bertrand@gmail.com.|Département de Médecine, Université de Montréal, Montreal, QC H3C 3J7, Canada. mariejeanne.bertrand@gmail.com.
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Bertrand
authors.affiliation: Département de Génie Électrique et Institut de Génie Biomédical, École Polytechnique de Montréal, Montreal, QC H3T 1J4, Canada. maxime.abran@gmail.com.|Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. maxime.abran@gmail.com.
authors.forename: Maxime
authors.initials: M
authors.lastname: Abran
authors.affiliation: Département de Génie Électrique et Institut de Génie Biomédical, École Polytechnique de Montréal, Montreal, QC H3T 1J4, Canada. alexandre.castonguay87@gmail.com.
authors.forename: Alexandre
authors.initials: A
authors.lastname: Castonguay
authors.affiliation: Département de Génie Électrique et Institut de Génie Biomédical, École Polytechnique de Montréal, Montreal, QC H3T 1J4, Canada. joel.lefebvre@gmail.com.
authors.forename: Joël
authors.initials: J
authors.lastname: Lefebvre
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. barbarastaehli@hotmail.com.
authors.forename: Barbara E
authors.initials: BE
authors.lastname: Stähli
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. nolwenn.merlet@gmail.com.
authors.forename: Nolwenn
authors.initials: N
authors.lastname: Merlet
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. Teodora.Mihalache-Avram@icm-mhi.org.
authors.forename: Teodora
authors.initials: T
authors.lastname: Mihalache-Avram
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. Pascale.Geoffroy@icm-mhi.org.
authors.forename: Pascale
authors.initials: P
authors.lastname: Geoffroy
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. melanie.mecteau@icm-mhi.org.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Mecteau
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. david.busseuil@icm-biobanque.org.
authors.forename: David
authors.initials: D
authors.lastname: Busseuil
authors.affiliation: National Research Council Canada (NRCC), Montreal, QC H3A 1A3, Canada. Feng.Ni@cnrc-nrc.gc.ca.
authors.forename: Feng
authors.initials: F
authors.lastname: Ni
authors.affiliation: National Research Council Canada (NRCC), Montreal, QC H3A 1A3, Canada. abedelnasser.abulrob@nrc-cnrc.gc.ca.
authors.forename: Abedelnasser
authors.initials: A
authors.lastname: Abulrob
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. Eric.Rheaume@icm-mhi.org.|Département de Médecine, Université de Montréal, Montreal, QC H3C 3J7, Canada. Eric.Rheaume@icm-mhi.org.
authors.forename: Éric
authors.initials: É
authors.lastname: Rhéaume
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. philippe.l_lallier@icloud.com.|Département de Médecine, Université de Montréal, Montreal, QC H3C 3J7, Canada. philippe.l_lallier@icloud.com.
authors.forename: Philippe
authors.initials: P
authors.lastname: L'Allier
authors.affiliation: Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. jean-claude.tardif@icm-mhi.org.|Département de Médecine, Université de Montréal, Montreal, QC H3C 3J7, Canada. jean-claude.tardif@icm-mhi.org.
authors.forename: Jean-Claude
authors.initials: JC
authors.lastname: Tardif
authors.affiliation: Département de Génie Électrique et Institut de Génie Biomédical, École Polytechnique de Montréal, Montreal, QC H3T 1J4, Canada. frederic.lesage@polymtl.ca.|Montreal Heart Institute, Montreal, QC H1T 1C8, Canada. frederic.lesage@polymtl.ca.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Lesage
mesh_terms: D000818:Animals; D000906:Antibodies / Q000378:metabolism; D016477:Artifacts; D001808:Blood Vessels / Q000473:pathology*; D057785:Catheters; D021621:Imaging, Three-Dimensional; D018799:Intercellular Adhesion Molecule-1 / Q000276:immunology; D008297:Male; D018613:Microscopy, Confocal / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D011817:Rabbits; D041623:Tomography, Optical Coherence / Q000379:methods*
chemical_list: D000906:Antibodies; D018799:Intercellular Adhesion Molecule-1
keywords: atherosclerosis; confocal fluorescence microscopy; ex vivo three-dimensional (3D) histology; intravascular ultrasound (IVUS); near-infrared fluorescence (NIRF); optical coherence tomography (OCT)