title: Differences in proteomic profiles of milk fat globule membrane in yak and cow milk.
abstract: Milk fat globule membrane (MFGM) is an important milk component which is rich in bioactive proteins. In this work, the isobaric tags for relative and absolute quantitation (iTRAQ) proteomic approach was used to investigate the differences in the MFGM proteins between yak and cow milk. Over 450 proteins were identified between the yak and cow MFGM. The MFGM proteins with significant differences were compared based on the relative abundance. Proteins such as Glycosylation-dependent cell adhesion molecule 1 (GlyCAM1), CD59 molecule and lactadherin, were identified having a much higher abundance (4.6-10.1 fold) in yak MFGM than cow MFGM. These proteins are thought to have biological functions such as the antimicrobial and antitumor effects. This may be due to the need that yak produces high nutritive milk including high levels of bioactive compounds in order to resist the extreme high altitude environment.
authors.affiliation: School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, Heilongjiang 150090, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: Ji
authors.affiliation: School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, Heilongjiang 150090, China.
authors.forename: Xisheng
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, Heilongjiang 150090, China. Electronic address: maying@hit.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Chemical Engineering and Technology, Harbin Institute of Technology, Harbin, Heilongjiang 150090, China; AgResearch Limited, Grasslands Research Centre, Tennent Drive, Palmerston North 4442, New Zealand. Electronic address: Li.Day@agresearch.co.nz.
authors.forename: Day
authors.initials: D
authors.lastname: Li
mesh_terms: D000818:Animals; D018957:CD59 Antigens / Q000737:chemistry; D002417:Cattle / Q000145:classification; D005260:Female; D006017:Glycolipids / Q000737:chemistry*; D006023:Glycoproteins / Q000737:chemistry*; D066292:Lipid Droplets; D008892:Milk / Q000737:chemistry*; D008894:Milk Proteins / Q000737:chemistry; D009077:Mucins / Q000737:chemistry; D040901:Proteomics
chemical_list: D018957:CD59 Antigens; D006017:Glycolipids; D006023:Glycoproteins; D008894:Milk Proteins; D009077:Mucins; C110367:milk fat globule; C075185:sulfated glycoprotein p50
keywords: Cow; MFGM; Proteins; Yak; iTRAQ