title: Xenogeneic Decellularized Scaffold: A Novel Platform for Ovary Regeneration.
abstract: Women younger than 40 years may face early menopause because of premature ovarian failure (POF). The cause of POF can be idiopathic or iatrogenic, especially the cancer-induced oophorectomy and chemo- or radiation therapy. The current treatments, including hormone replacement therapy (HRT) and cryopreservation techniques, have increased risk of ovarian cancer and may reintroduce malignant cells after autografting. Decellularization technique has been regarded as a novel regenerative medicine strategy for organ replacement, wherein the living cells of an organ are removed, leaving the extracellular matrix (ECM) for cellular seeding. This study aimed to produce a xenogeneic decellularized ovary (D-ovary) scaffold as a platform for ovary regeneration and transplantation. We have developed a novel decellularization protocol for porcine ovary by treatment with physical, chemical, and enzymatic methods. Using hematoxylin and eosin (H&E) staining, DAPI staining, scanning electron microscopy (SEM), and quantitative analysis, this approach proved effective in removing cellular components and preserving ECM. Furthermore, the results of biological safety evaluation demonstrated that the D-ovary tissues were noncytotoxic for rat ovarian cells in vitro and caused only a minimal immunogenic response in vivo. In addition, the D-ovary tissues successfully supported rat granulosa cell penetration ex vivo and showed an improvement in estradiol (E2) hormone secretion.
authors.affiliation: 1 Department of Endocrinology and Metabolism, the First Affiliated Hospital of Wenzhou Medical University , Wenzhou, China .
authors.forename: Wen-Yue
authors.initials: WY
authors.lastname: Liu
authors.affiliation: 2 School of the First Clinical Medical Sciences, Wenzhou Medical University , Wenzhou, China .
authors.forename: Shi-Gang
authors.initials: SG
authors.lastname: Lin
authors.affiliation: 1 Department of Endocrinology and Metabolism, the First Affiliated Hospital of Wenzhou Medical University , Wenzhou, China .
authors.forename: Ru-Yi
authors.initials: RY
authors.lastname: Zhuo
authors.affiliation: 1 Department of Endocrinology and Metabolism, the First Affiliated Hospital of Wenzhou Medical University , Wenzhou, China .
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Xie
authors.affiliation: 1 Department of Endocrinology and Metabolism, the First Affiliated Hospital of Wenzhou Medical University , Wenzhou, China .
authors.forename: Wei
authors.initials: W
authors.lastname: Pan
authors.affiliation: 3 Department of Orthopaedic Surgery, Sir Run Run Shaw Hospital, Medical College of Zhejiang University , Hangzhou, China .
authors.forename: Xian-Feng
authors.initials: XF
authors.lastname: Lin
authors.affiliation: 1 Department of Endocrinology and Metabolism, the First Affiliated Hospital of Wenzhou Medical University , Wenzhou, China .
authors.forename: Fei-Xia
authors.initials: FX
authors.lastname: Shen
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004958:Estradiol / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism*; D005260:Female; D010053:Ovary / Q000166:cytology*; D051381:Rats; D012038:Regeneration / Q000502:physiology*; D044968:Regenerative Medicine; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D004958:Estradiol
keywords: bioengineering in organ transplantation; decellularization; extracellular matrix; premature ovarian failure; regenerative medicine