title: Fabrication of Surface Protein-Imprinted Biofuel Cell for Sensitive Self-Powered Glycoprotein Detection.
abstract: Glycoproteins are important biomarkers and therapeutic targets in clinical diagnostics. The conventional analytical methods for glycoprotein are usually faced with some challenges, such as the complex pretreatment of samples, poor availability, and limited stability of antibody, making them not suitable for point-of-care and on-site application. Herein, we demonstrate a novel miniaturized biofuel cells (BFCs)-based self-powered nanosensor for the specific and sensitive determination of glycoproteins in complex samples through the combination of boronate-affinity molecularly imprinted polymer (MIP) and the boronate affinity functionalized biliroxidase-carbon nanotube nanocomposites. The above MIP and the nanocomposites act as both signal probe and biocatalyst at the cathode. The as-obtained self-powered MIP-BFC-based biosensor can detect horseradish peroxidase (a type of glycoprotein) with a wide linear range of 1 ng/mL to 10 μg/mL and a very low detection limit of 1 ng/mL. Especially, it shows high tolerance for different interferences (e.g., sugars and other glycoproteins) and can even measure the α-fetoprotein level in serum samples. Moreover, it exhibits significant advantages over the conventional assays in terms of cost efficiency, stability, and speed, especially inexpensive instrument needed. Our novel approach for construction of the sensor paves a simple and economical way to fabricate portable devices for point-of-care and on-site application.
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan 410083, P.R. China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Ouyang
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan 410083, P.R. China.
authors.forename: Zhenjun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan 410083, P.R. China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Han
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan 410083, P.R. China.
authors.forename: Ke
authors.initials: K
authors.lastname: Zeng
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan 410083, P.R. China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Sheng
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan 410083, P.R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Deng
authors.affiliation: College of Chemistry and Chemical Engineering, Central South University , Changsha, Hunan 410083, P.R. China.
authors.forename: You-Nian
authors.identifier: http://orcid.org/0000-0002-7078-5937
authors.initials: YN
authors.lastname: Liu
mesh_terms: D001674:Bioelectric Energy Sources; D015374:Biosensing Techniques; D006023:Glycoproteins; D008565:Membrane Proteins; D054802:Molecular Imprinting; D037742:Nanotubes, Carbon
chemical_list: D006023:Glycoproteins; D008565:Membrane Proteins; D037742:Nanotubes, Carbon
keywords: biofuel cell; biosensor; glycoprotein; protein-imprinted polymer; self-power