title: Naa50/San-dependent N-terminal acetylation of Scc1 is potentially important for sister chromatid cohesion.
abstract: The gene separation anxiety (san) encodes Naa50/San, a N-terminal acetyltransferase required for chromosome segregation during mitosis. Although highly conserved among higher eukaryotes, the mitotic function of this enzyme is still poorly understood. Naa50/San was originally proposed to be required for centromeric sister chromatid cohesion in Drosophila and human cells, yet, more recently, it was also suggested to be a negative regulator of microtubule polymerization through internal acetylation of beta Tubulin. We used genetic and biochemical approaches to clarify the function of Naa50/San during development. Our work suggests that Naa50/San is required during tissue proliferation for the correct interaction between the cohesin subunits Scc1 and Smc3. Our results also suggest a working model where Naa50/San N-terminally acetylates the nascent Scc1 polypeptide, and that this co-translational modification is subsequently required for the establishment and/or maintenance of sister chromatid cohesion.
authors.affiliation: Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.|Center for Biomedical Research (CBMR), University of Algarve, 8005-139 Faro, Portugal.
authors.forename: Ana Luisa
authors.initials: AL
authors.lastname: Ribeiro
authors.affiliation: Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.|Center for Biomedical Research (CBMR), University of Algarve, 8005-139 Faro, Portugal.
authors.forename: Rui D
authors.initials: RD
authors.lastname: Silva
authors.affiliation: Department of Molecular Biology, University of Bergen, N-5020 Bergen, Norway.
authors.forename: Håvard
authors.initials: H
authors.lastname: Foyn
authors.affiliation: Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.|Center for Biomedical Research (CBMR), University of Algarve, 8005-139 Faro, Portugal.
authors.forename: Margarida N
authors.initials: MN
authors.lastname: Tiago
authors.affiliation: Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.|Center for Biomedical Research (CBMR), University of Algarve, 8005-139 Faro, Portugal.
authors.forename: Om Singh
authors.initials: OS
authors.lastname: Rathore
authors.affiliation: Department of Molecular Biology, University of Bergen, N-5020 Bergen, Norway.|Department of Surgery, Haukeland University Hospital, N-5021 Bergen, Norway.
authors.forename: Thomas
authors.initials: T
authors.lastname: Arnesen
authors.affiliation: Departamento de Ciências Biomédicas e Medicina, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.|Center for Biomedical Research (CBMR), University of Algarve, 8005-139 Faro, Portugal.|Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisboa, Portugal.
authors.forename: Rui Gonçalo
authors.initials: RG
authors.lastname: Martinho
mesh_terms: D000107:Acetylation; D000123:Acetyltransferases / Q000378:metabolism*; D000251:Adenosine Triphosphatases / Q000378:metabolism; D000818:Animals; D018797:Cell Cycle Proteins / Q000737:chemistry* / Q000378:metabolism*; D002460:Cell Line; D049109:Cell Proliferation; D002842:Chromatids / Q000235:genetics*; D020090:Chromosome Segregation; D029721:Drosophila Proteins / Q000737:chemistry* / Q000378:metabolism*; D004331:Drosophila melanogaster / Q000254:growth & development* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D014921:Wings, Animal / Q000254:growth & development / Q000378:metabolism
chemical_list: D018797:Cell Cycle Proteins; D029721:Drosophila Proteins; C410884:vtd protein, Drosophila; D000123:Acetyltransferases; C533244:San protein, Drosophila; D000251:Adenosine Triphosphatases; C541514:SMC3 protein, Drosophila