title: Degradation kinetics of peptide-coupled alginates prepared via the periodate oxidation reductive amination route.
abstract: Biomaterials based on peptide-coupled alginates must provide both optimal biological environments and tuneable stability/degradation profiles. The present work investigates the degradation pattern and kinetics of peptide-coupled alginates prepared via the periodate oxidation reductive amination route. Alginates degrade slowly (non-enzymatically) under physiological conditions by acid-catalysed hydrolysis and alkali-catalysed β-elimination, both operating simultaneously but dominated by the latter. While periodate oxidation alone largely increases the rate of β-elimination, substitution restores the susceptibility towards β-elimination to that of the parent alginate. For acid hydrolysis the rate of depolymerization is proportional to the degree of substitution, being approximately one order of magnitude larger than the parent alginate, but still lower than for the corresponding materials with fully reduced dialdehydes. Results also suggest a composition-dependent preference for substitution at C2 or C3. These results demonstrate how the various chemistries introduced by the coupling provide useful means to tune the biodegradability profiles.
authors.affiliation: NOBIPOL, Department of Biotechnology, NTNU Norwegian University of Science and Technology, N-7491 Trondheim, Norway. Electronic address: marianne.dalheim@ntnu.no.
authors.forename: Marianne Ø
authors.initials: MØ
authors.lastname: Dalheim
authors.affiliation: NOBIPOL, Department of Biotechnology, NTNU Norwegian University of Science and Technology, N-7491 Trondheim, Norway. Electronic address: ann-sissel.t.ulset@ntnu.no.
authors.forename: Ann-Sissel T
authors.initials: AT
authors.lastname: Ulset
authors.affiliation: NOBIPOL, Department of Biotechnology, NTNU Norwegian University of Science and Technology, N-7491 Trondheim, Norway. Electronic address: ina.b.jenssen@ntnu.no.
authors.forename: Ina Beate
authors.initials: IB
authors.lastname: Jenssen
authors.affiliation: NOBIPOL, Department of Biotechnology, NTNU Norwegian University of Science and Technology, N-7491 Trondheim, Norway. Electronic address: bjorn.e.christensen@ntnu.no.
authors.forename: Bjørn E
authors.initials: BE
authors.lastname: Christensen
mesh_terms: D000464:Alginates / Q000378:metabolism*; D000586:Amination; D007700:Kinetics; D010084:Oxidation-Reduction; D010455:Peptides / Q000378:metabolism*; D010504:Periodic Acid
chemical_list: D000464:Alginates; D010455:Peptides; D010504:Periodic Acid; C009288:metaperiodate
keywords: Acid hydrolysis; Alginate; Periodate oxidation; Reductive amination; SEC-MALS; β-Elimination