title: Identification and characterization of host cell proteins interacting with Scylla serrata reovirus non-structural protein p35.
abstract: We have previously shown that non-structural protein p35, encoded by Scylla serrata reovirus (SsRV) S10, may act as a viroporin. To characterize the role of p35 protein in the modulation of cellular function, a yeast two-hybrid system was used to screen a cDNA library derived from S. serrata to find its interacting partner. Protein interactions were confirmed in vitro by GST pull-down. Full cDNAs of p35 interactors were cloned by the rapid amplification of cDNA ends. After two-hybrid library screening, we isolated partial cDNAs encoding hemocyanin, cryptocyanin, and TAX1BP1. Interaction of p35 with each of hemocyanin, cryptocyanin, and TAX1BP1 was confirmed by GST pull-down. The full-length cDNA fragments of hemocyanin, cryptocyanin, and TAX1BP1 were 2287, 2422, and 3437 bp, respectively, and they encoded three putative proteins with molecular masses of ~76.9, ~79.2, and ~107.2 kDa, respectively. This study casts new light on the function and physiological significance of p35 during the SsRV replication cycle.
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of China.
authors.forename: Dongyang
authors.initials: D
authors.lastname: Fan
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of China.
authors.forename: Sidong
authors.initials: S
authors.lastname: Zhu
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of China.
authors.forename: Jifang
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Biological and Environmental Sciences, Zhejiang Wanli University, No.8, South Qianhu Road, Ningbo, Zhejiang Province, 315100, People's Republic of China. genomic@163.com.
authors.forename: Jigang
authors.initials: J
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D003386:Brachyura / Q000821:virology*; D018076:DNA, Complementary / Q000235:genetics; D054884:Host-Pathogen Interactions / Q000235:genetics*; D008969:Molecular Sequence Data; D010802:Phylogeny; D012087:Reoviridae / Q000235:genetics* / Q000472:pathogenicity; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism
chemical_list: D018076:DNA, Complementary; D017361:Viral Nonstructural Proteins
keywords: Cryptocyanin; Hemocyanin; Scylla serrata reovirus (SsRV); TAX1BP1; Yeast two-hybrid system