title: Epithelial ovarian carcinoma diagnosis by desorption electrospray ionization mass spectrometry imaging.
abstract: Ovarian cancer is highly prevalent among European women, and is the leading cause of gynaecological cancer death. Current histopathological diagnoses of tumour severity are based on interpretation of, for example, immunohistochemical staining. Desorption electrospray mass spectrometry imaging (DESI-MSI) generates spatially resolved metabolic profiles of tissues and supports an objective investigation of tumour biology. In this study, various ovarian tissue types were analysed by DESI-MSI and co-registered with their corresponding haematoxylin and eosin (H&E) stained images. The mass spectral data reveal tissue type-dependent lipid profiles which are consistent across the n = 110 samples (n = 107 patients) used in this study. Multivariate statistical methods were used to classify samples and identify molecular features discriminating between tissue types. Three main groups of samples (epithelial ovarian carcinoma, borderline ovarian tumours, normal ovarian stroma) were compared as were the carcinoma histotypes (serous, endometrioid, clear cell). Classification rates >84% were achieved for all analyses, and variables differing statistically between groups were determined and putatively identified. The changes noted in various lipid types help to provide a context in terms of tumour biochemistry. The classification of unseen samples demonstrates the capability of DESI-MSI to characterise ovarian samples and to overcome existing limitations in classical histopathology.
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Maria Luisa
authors.initials: ML
authors.lastname: Dória
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: James S
authors.initials: JS
authors.lastname: McKenzie
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Anna
authors.initials: A
authors.lastname: Mroz
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: David L
authors.initials: DL
authors.lastname: Phelps
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Abigail
authors.initials: A
authors.lastname: Speller
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Francesca
authors.initials: F
authors.lastname: Rosini
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Nicole
authors.initials: N
authors.lastname: Strittmatter
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Ottmar
authors.initials: O
authors.lastname: Golf
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Kirill
authors.initials: K
authors.lastname: Veselkov
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Robert
authors.initials: R
authors.lastname: Brown
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Sadaf
authors.initials: S
authors.lastname: Ghaem-Maghami
authors.affiliation: Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, London, United Kingdom.
authors.forename: Zoltan
authors.initials: Z
authors.lastname: Takats
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000175:diagnosis* / Q000473:pathology; D018269:Carcinoma, Endometrioid / Q000175:diagnosis* / Q000473:pathology; D016002:Discriminant Analysis; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D010051:Ovarian Neoplasms / Q000175:diagnosis* / Q000473:pathology; D010713:Phosphatidylcholines / Q000032:analysis; D010714:Phosphatidylethanolamines / Q000032:analysis; D010716:Phosphatidylinositols / Q000032:analysis; D010718:Phosphatidylserines / Q000032:analysis; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D010713:Phosphatidylcholines; D010714:Phosphatidylethanolamines; D010716:Phosphatidylinositols; D010718:Phosphatidylserines; C483858:phosphatidylethanolamine