title: Zoonotic Cryptosporidium Species in Animals Inhabiting Sydney Water Catchments.
abstract: Cryptosporidium is one of the most common zoonotic waterborne parasitic diseases worldwide and represents a major public health concern of water utilities in developed nations. As animals in catchments can shed human-infectious Cryptosporidium oocysts, determining the potential role of animals in dissemination of zoonotic Cryptosporidium to drinking water sources is crucial. In the present study, a total of 952 animal faecal samples from four dominant species (kangaroos, rabbits, cattle and sheep) inhabiting Sydney's drinking water catchments were screened for the presence of Cryptosporidium using a quantitative PCR (qPCR) and positives sequenced at multiple loci. Cryptosporidium species were detected in 3.6% (21/576) of kangaroos, 7.0% (10/142) of cattle, 2.3% (3/128) of sheep and 13.2% (14/106) of rabbit samples screened. Sequence analysis of a region of the 18S rRNA locus identified C. macropodum and C. hominis in 4 and 17 isolates from kangaroos respectively, C. hominis and C. parvum in 6 and 4 isolates respectively each from cattle, C. ubiquitum in 3 isolates from sheep and C. cuniculus in 14 isolates from rabbits. All the Cryptosporidium species identified were zoonotic species with the exception of C. macropodum. Subtyping using the 5' half of gp60 identified C. hominis IbA10G2 (n = 12) and IdA15G1 (n = 2) in kangaroo faecal samples; C. hominis IbA10G2 (n = 4) and C. parvum IIaA18G3R1 (n = 4) in cattle faecal samples, C. ubiquitum subtype XIIa (n = 1) in sheep and C. cuniculus VbA23 (n = 9) in rabbits. Additional analysis of a subset of samples using primers targeting conserved regions of the MIC1 gene and the 3' end of gp60 suggests that the C. hominis detected in these animals represent substantial variants that failed to amplify as expected. The significance of this finding requires further investigation but might be reflective of the ability of this C. hominis variant to infect animals. The finding of zoonotic Cryptosporidium species in these animals may have important implications for the management of drinking water catchments to minimize risk to public health.
authors.affiliation: Vector and Waterborne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Australia.
authors.forename: Alireza
authors.initials: A
authors.lastname: Zahedi
authors.affiliation: Australian Water Quality Centre, South Australian Water Corporation, Adelaide, Australia.|School of Medicine, Flinders University, Bedford Park, Australia.
authors.forename: Paul
authors.initials: P
authors.lastname: Monis
authors.affiliation: School of Medicine, Flinders University, Bedford Park, Australia.
authors.forename: Sarah
authors.initials: S
authors.lastname: Aucote
authors.affiliation: Australian Water Quality Centre, South Australian Water Corporation, Adelaide, Australia.|School of Medicine, Flinders University, Bedford Park, Australia.
authors.forename: Brendon
authors.initials: B
authors.lastname: King
authors.affiliation: Vector and Waterborne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Australia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Paparini
authors.affiliation: Henan Agricultural University, Henan, China.
authors.forename: Fuchun
authors.initials: F
authors.lastname: Jian
authors.affiliation: Vector and Waterborne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Australia.
authors.forename: Rongchang
authors.initials: R
authors.lastname: Yang
authors.affiliation: Vector and Waterborne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Australia.
authors.forename: Charlotte
authors.initials: C
authors.lastname: Oskam
authors.affiliation: WaterNSW, Sydney, Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Ball
authors.affiliation: Vector and Waterborne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Australia.
authors.forename: Ian
authors.initials: I
authors.lastname: Robertson
authors.affiliation: Vector and Waterborne Pathogen Research Group, School of Veterinary and Life Sciences, Murdoch University, Perth, Australia.
authors.forename: Una
authors.initials: U
authors.lastname: Ryan
mesh_terms: D000818:Animals; D002417:Cattle / Q000469:parasitology; D002418:Cattle Diseases / Q000469:parasitology; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000235:genetics / Q000302:isolation & purification*; D016054:DNA, Protozoan / Q000235:genetics; D005243:Feces / Q000469:parasitology; D006801:Humans; D007614:Macropodidae / Q000469:parasitology; D008853:Microscopy; D009517:New South Wales; D033661:Oocysts; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D011817:Rabbits / Q000469:parasitology; D012756:Sheep / Q000469:parasitology; D014867:Water; D014873:Water Pollutants / Q000032:analysis; D014881:Water Supply; D015047:Zoonoses / Q000469:parasitology
chemical_list: D016054:DNA, Protozoan; D012337:RNA, Ribosomal, 18S; D014873:Water Pollutants; D014867:Water