title: Optical Fiber-Type Sugar Chip Using Localized Surface Plasmon Resonance.
abstract: Optical fiber-type Sugar Chips were developed using localized surface plasmon resonance (LSPR) of gold (Au) nanoparticles. The endface of an optical fiber was first aminosilylated and then condensed with α-lipoic acid containing a dithiol group. Second, gold nanoparticles were immobilized onto the endface via an Au-S covalent bond. Finally, sugar moieties were attached to the gold nanoparticle using our original sugar chain-ligand conjugates to obtain fiber-type Sugar Chips, by which the sugar moiety-protein interaction was analyzed. The specificity, sensitivity, and quantitative binding potency against carbohydrate-binding protein were found to be identical to that of a conventional SPR sensor. In this analysis, only a small sample volume (approximately 10 μL) was required compared with 100 μL for the conventional SPR sensor, suggesting that the fiber-type Sugar Chip and LSPR are applicable for nonpure small masses of proteins.
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.
authors.forename: Masahiro
authors.identifier: 0000-0003-2554-8825
authors.initials: M
authors.lastname: Wakao
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Watanabe
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.
authors.forename: Yoshie
authors.initials: Y
authors.lastname: Kurahashi
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.
authors.forename: Takahide
authors.initials: T
authors.lastname: Matsuo
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takeuchi
authors.affiliation: Moritex Corporation , 1-3-3 Azamino-minami, Aobaku, Yokohama, Kanagawa 225-0012, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Ogawa
authors.affiliation: Moritex Corporation , 1-3-3 Azamino-minami, Aobaku, Yokohama, Kanagawa 225-0012, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Moritex Corporation , 1-3-3 Azamino-minami, Aobaku, Yokohama, Kanagawa 225-0012, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Yumino
authors.affiliation: Moritex Corporation , 1-3-3 Azamino-minami, Aobaku, Yokohama, Kanagawa 225-0012, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Myogadani
authors.affiliation: Moritex Corporation , 1-3-3 Azamino-minami, Aobaku, Yokohama, Kanagawa 225-0012, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Saito
authors.affiliation: Moritex Corporation , 1-3-3 Azamino-minami, Aobaku, Yokohama, Kanagawa 225-0012, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Muta
authors.affiliation: Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Midoriku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Mitsunori
authors.initials: M
authors.lastname: Kimura
authors.affiliation: Interdisciplinary Graduate School of Science and Engineering, Tokyo Institute of Technology , Midoriku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Kajikawa
authors.affiliation: Department of Chemistry, Biotechnology, and Chemical Engineering, Graduate School of Science and Engineering, Kagoshima University , 1-21-40 Kohrimoto, Kagoshima, Kagoshima 890-0065, Japan.|SUDx-Biotec Corporation , 1-42-1 Shiroyama, Kagoshima, Kagoshima 890-0013, Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Suda
mesh_terms: D004867:Equipment Design; D006001:Glycoconjugates / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D037102:Lectins / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D055100:Optical Fibers; D020349:Surface Plasmon Resonance / Q000295:instrumentation*; D008063:Thioctic Acid / Q000737:chemistry; D014050:Toluene / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D006001:Glycoconjugates; D037102:Lectins; D014050:Toluene; D008063:Thioctic Acid; D006046:Gold; C004848:dithiol