title: Immunohistological analysis of eotaxin and RANTES in the model animal of eosinophilic otitis media.
abstract: BACKGROUND
  The most crucial clinical problem of Eosinophilic Otitis Media (EOM) is sensorineural hearing loss. A previous report revealed that repeated antigen stimulation of middle ear causes eosinophilic inflammation not only in the middle ear but also in the inner ear.
  
  
  OBJECTIVE
  The purpose of the present study was to elucidate the mechanism of eosinophil infiltration to the inner ear in the animal model of EOM.
  
  
  METHODS
  Continuous OVA stimulation to the middle ear of guinea pigs was performed for 7 days, 14 days, and 28 days. Histological observation was made for eosinophil infiltration and morphological change of the inner ear. Immunostaining for eotaxin and RANTES was performed to study immunoreactivity of those chemokines.
  
  
  RESULTS
  In the 7-day stimulation side, a few eosinophils were found in the scala tympani, without obvious morphological damage of the inner ear. Moreover, immunoreactivity of both eotaxin and RANTES was significantly higher in the OVA stimulation sides than control sides. For both eotaxin and RANTES, the number of immunopositive cells was significantly increased in the 14-day stimulation side over the 7-day stimulation side.
  
  
  CONCLUSIONS
  Eotaxin and RANTES seem to play some important roles for the eosinophil infiltration in the middle and inner ear of model animal of EOM.
authors.affiliation: a Department of Otorhinolaryngology , Hirosaki University Graduate School of Medicine , Hirosaki , Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Kudo
authors.affiliation: a Department of Otorhinolaryngology , Hirosaki University Graduate School of Medicine , Hirosaki , Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Matsubara
authors.affiliation: a Department of Otorhinolaryngology , Hirosaki University Graduate School of Medicine , Hirosaki , Japan.
authors.forename: Hisanori
authors.initials: H
authors.lastname: Nishizawa
authors.affiliation: a Department of Otorhinolaryngology , Hirosaki University Graduate School of Medicine , Hirosaki , Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Miura
mesh_terms: D000818:Animals; D054413:Chemokine CCL11 / Q000378:metabolism; D018946:Chemokine CCL5 / Q000378:metabolism*; D004195:Disease Models, Animal; D007758:Ear, Inner / Q000276:immunology* / Q000378:metabolism; D004432:Ear, Middle / Q000378:metabolism; D004802:Eosinophilia / Q000276:immunology* / Q000378:metabolism; D006168:Guinea Pigs; D010033:Otitis Media / Q000276:immunology* / Q000378:metabolism; D013701:Temporal Bone / Q000378:metabolism
chemical_list: D054413:Chemokine CCL11; D018946:Chemokine CCL5
keywords: Eosinophil; RANTES; eotaxin; round window membrane; scala tympani