title: Genomic Insights and Its Comparative Analysis withYersinia enterocoliticaReveals the Potential Virulence Determinants and Further Pathogenicity for Foodborne Outbreaks.
abstract: Yersinia enterocoliticais a well-known foodborne pathogen causing gastrointestinal infections worldwide. The strainY. enterocoliticaFORC_002 was isolated from the gill of flatfish (plaice) and its genome was sequenced. The genomic DNA consists of 4,837,317 bp with a GC content of 47.1%, and is predicted to contain 4,221 open reading frames, 81 tRNA genes, and 26 rRNA genes. Interestingly, genomic analysis revealed pathogenesis and host immune evasion-associated genes encoding guanylate cyclase (Yst), invasin (Ail and Inv), outer membrane protein (Yops), autotransporter adhesin A (YadA), RTX-like toxins, and a type III secretion system. In particular, guanylate cyclase is a heat-stable enterotoxin causingYersinia-associated diarrhea, and RTX-like toxins are responsible for attachment to integrin on the target cell for cytotoxic action. This genome can be used to identify virulence factors that can be applied for the development of novel biomarkers for the rapid detection of this pathogen in foods.
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Gopalsamy
authors.initials: G
authors.lastname: Gnanasekaran
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Eun Jung
authors.initials: EJ
authors.lastname: Na
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Han Young
authors.initials: HY
authors.lastname: Chung
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Suyeon
authors.initials: S
authors.lastname: Kim
authors.affiliation: Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: You-Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Woori
authors.initials: W
authors.lastname: Kwak
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Heebal
authors.initials: H
authors.lastname: Kim
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
authors.affiliation: Department of Agricultural Biotechnology, Seoul National University, Seoul 08826, Republic of Korea.|Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sang Ho
authors.initials: SH
authors.lastname: Choi
authors.affiliation: Food-borne Pathogen Omics Research Center (FORC), Seoul National University, Seoul 08826, Republic of Korea.|Department of Food Science and Biotechnology, Kyung Hee University, Yongin 17104, Republic of Korea.
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
mesh_terms: D018829:Adhesins, Bacterial / Q000235:genetics; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000235:genetics; D001426:Bacterial Proteins / Q000235:genetics; D001427:Bacterial Toxins / Q000235:genetics; D015415:Biomarkers; D003546:Cysteine Endopeptidases / Q000235:genetics; D004196:Disease Outbreaks; D004768:Enterotoxins / Q000235:genetics; D005412:Flatfishes / Q000382:microbiology; D005517:Foodborne Diseases / Q000175:diagnosis / Q000382:microbiology; D005759:Gastroenteritis / Q000453:epidemiology / Q000382:microbiology*; D016680:Genome, Bacterial; D023281:Genomics; D006801:Humans; D057131:Immune Evasion / Q000235:genetics; D046529:Microscopy, Electron, Transmission; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D000069296:Type III Secretion Systems / Q000235:genetics; D037521:Virulence Factors / Q000235:genetics*; D015009:Yersinia Infections / Q000453:epidemiology / Q000382:microbiology*; D015008:Yersinia enterocolitica / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity* / Q000648:ultrastructure
chemical_list: D018829:Adhesins, Bacterial; C069712:Ail protein, Yersinia enterocolitica; D001425:Bacterial Outer Membrane Proteins; D001426:Bacterial Proteins; D001427:Bacterial Toxins; D015415:Biomarkers; D004768:Enterotoxins; D000069296:Type III Secretion Systems; D037521:Virulence Factors; C064832:YadA protein, Yersinia; C116117:YopT protein, Yersinia; C048669:heat-stable enterotoxin (Yersinia); D003546:Cysteine Endopeptidases
keywords: Type III secretion system; Yersinia enterocolitica; food poisoning; gastroenteritis; genome; virulence factor