title: Influence of tetramethylenedisulfotetramine on synchronous calcium oscillations at distinct developmental stages of hippocampal neuronal cultures.
abstract: The spatial and temporal patterns of spontaneous synchronous Ca2+oscillations (SCOs) regulate physiological pathways that influence neuronal development, excitability, and health. Hippocampal neuronal cultures (HN) and neuron/glia co-cultures (HNG) produced from neonatal mice were loaded with Fluo-4/AM and SCOs recorded in real-time using a Fluorescence Imaging Plate Reader at different developmental stages in vitro. HNG showed an earlier onset of SCOs, with low amplitude and low frequency SCOs at 4days in vitro (DIV), whereas HN were quiescent at this point. SCO amplitude peaked at 9 DIV for both cultures. SCO network frequency peaked at 12 DIV in HN, whereas in HNG the frequency peaked at 6 DIV. SCO patterns were associated with the temporal development of neuronal networks and their ratio of glutamatergic to GABAergic markers of excitatory/inhibitory balance. HN and HNG exhibited differential responses to the convulsant tetramethylenedisulfotetramine (TETS) and were highly dependent on DIV. In HN, TETS triggered an acute rise of intracellular Ca2+(Phase I response) only in 14 DIV and a sustained decrease of SCO frequency with increased amplitude (Phase II response) at all developmental stages. In HNG, TETS decreased the SCO frequency and increased the amplitude at 6 and 14 but not 9 DIV. There was no acute Ca2+rise (Phase I response) in any age of HNG tested with TETS. These data demonstrated the importance of glia and developmental stage in modulating neuronal responses to TETS. Our results illustrate the applicability of the model for investigating how caged convulsants elicit abnormal network activity during the development of HN and HNG cultures in vitro.
authors.affiliation: State Key Laboratory of Natural Medicines & Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Development, China Pharmaceutical University, Nanjing, 211198, PR China; Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, 95616, United States. Electronic address: zycao1999@hotmail.com.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Natural Medicines & Jiangsu Provincial Key Laboratory for TCM Evaluation and Translational Development, China Pharmaceutical University, Nanjing, 211198, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, 95616, United States.
authors.forename: Susan
authors.initials: S
authors.lastname: Hulsizer
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, 95616, United States.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, 95616, United States.
authors.forename: Yao
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Molecular Biosciences, School of Veterinary Medicine, University of California, Davis, 95616, United States. Electronic address: inpessah@ucdavis.edu.
authors.forename: Isaac N
authors.initials: IN
authors.lastname: Pessah
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001952:Bridged-Ring Compounds / Q000494:pharmacology*; D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling / Q000187:drug effects*; D018920:Coculture Techniques; D003292:Convulsants / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D018756:GABA Antagonists / Q000494:pharmacology; D005786:Gene Expression Regulation / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein; D006624:Hippocampus / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000502:physiology; D009474:Neurons / Q000187:drug effects*; D010852:Picrotoxin / Q000494:pharmacology; D018080:Receptors, GABA-B / Q000378:metabolism; D013997:Time Factors; D050598:Vesicular Glutamate Transport Protein 1 / Q000378:metabolism
chemical_list: D001952:Bridged-Ring Compounds; D002118:Calcium; D003292:Convulsants; D018756:GABA Antagonists; D005904:Glial Fibrillary Acidic Protein; D008869:Microtubule-Associated Proteins; D010852:Picrotoxin; D018080:Receptors, GABA-B; D050598:Vesicular Glutamate Transport Protein 1; C010349:tetramethylenedisulfotetramine
keywords: Calcium oscillations; Hippocampal neuronal network; Tetramethylenedisulfotetramine