title: Scutellarin suppresses migration and invasion of human hepatocellular carcinoma by inhibiting the STAT3/Girdin/Akt activity.
abstract: Scutellarin is an active flavone from Erigeron breviscapine (vant) Hand Mass. This study aimed to investigate the potential role of scutellarin in migration and invasion of human hepatocellular carcinoma (HCC) cells and its possible mechanism. In comparison with the vehicle-treated controls, treatment with scutellarin (50 mg/kg/day) for 35 days significantly mitigated the lung and intrahepatic metastasis of HCC tumors in vivo. Scutellarin treatment significantly reduced HepG2 cell viability in a dose-dependent manner, and inhibited migration and invasion of HCC cells in vitro. Scutellarin treatment significantly reduced STAT3 and Girders of actin filaments (Girdin) expression, STAT3 and Akt phosphorylation in HCC cells. Introduction of STAT3 overexpression restored the scutellarin-downregulated Girdin expression, Akt activation, migration and invasion of HCC cells. Furthermore, induction of Girdin overexpression completely abrogated the inhibition of scutellarin on the Akt phosphorylation, migration and invasion of HCC cells. Scutellarin can inhibit HCC cell metastasis in vivo, and migration and invasion in vitro by down-regulating the STAT3/Girdin/Akt signaling.
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Ke
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China; Mental Health Center, Kunming Medical University, Kunming, China.
authors.forename: Tianhao
authors.initials: T
authors.lastname: Bao
authors.affiliation: Department of Gastroenterological Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Gastroenterological Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Haoran
authors.initials: H
authors.lastname: Tang
authors.affiliation: Department of Pathology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Jiayun
authors.initials: J
authors.lastname: Ge
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Bimang
authors.initials: B
authors.lastname: Fu
authors.affiliation: Intensive Care Unit, The First Hospital of Kunming, Kunming, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Meng
authors.affiliation: Department of Pediatric Surgery, The Kunming Children's Hospital, Kunming, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Yuqi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Haotian
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Zhitang
authors.initials: Z
authors.lastname: Guo
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Ni
authors.affiliation: Department of Oncology, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Xuefen
authors.initials: X
authors.lastname: Lei
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Zhitian
authors.initials: Z
authors.lastname: Shi
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Wei
authors.affiliation: Department of Hepatobiliary Surgery, The Second Affiliated Hospital of Kunming Medical University, Kunming, China. Electronic address: linwang0705@126.com.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D047310:Apigenin / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005965:Glucuronates / Q000494:pharmacology*; D056945:Hep G2 Cells / Q000187:drug effects / Q000473:pathology; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D008840:Microfilament Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D051057:Proto-Oncogene Proteins c-akt / Q000037:antagonists & inhibitors / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000037:antagonists & inhibitors* / Q000378:metabolism; D033921:Vesicular Transport Proteins / Q000037:antagonists & inhibitors* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D000972:Antineoplastic Agents, Phytogenic; C502451:CCDC88A protein, human; D005965:Glucuronates; D008840:Microfilament Proteins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; D033921:Vesicular Transport Proteins; C484876:scutellarin; D047310:Apigenin; D051057:Proto-Oncogene Proteins c-akt
keywords: Akt; Girdin; Hepatocellular carcinoma; Invasion; STAT3; Scutellarin