title: Differential expression of peroxiredoxin 3 in laryngeal squamous cell carcinoma.
abstract: Peroxiredoxin (PRDX) proteins are involved in carcinogenesis. PRDX3, which is predominantly localized in mitochondria and up-regulated in several human cancers, seems to confer increased treatment resistance and aggressive phenotypes. This study examined the expression profile of PRDX3 and its possible clinical value in laryngeal squamous cell carcinoma (LSCC). The expression of PRDX3 in LSCC samples was confirmed by Western blotting and further analyzed by immunohistochemistry in LSCC samples of different clinical pathological stages. The results showed that up-regulated expression of PRDX3 was observed in LSCC and associated with poor differentiation (P < 0.01), primary tumor location, N category and tumor stage (P < 0.05). Knockdown of PRDX3 in the Hep-2 laryngeal carcinoma epithelial cell line significantly enhanced Hep-2 cells' apoptosis and inhibited their proliferation and migration. Taken together, our results suggest that PRDX3 has substantial clinical impact on the progression of LSCC and shed new light on the role of PRDX3 in treatment resistance and aggressive phenotypes in LSCC.
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Yuhuangding Hospital of Qingdao University, Yantai, China.|Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Central Laboratory, Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Pathology, Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Li
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Central Laboratory, Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Department of Central Laboratory, Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhu
authors.affiliation: Department of Central Laboratory, Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Yuhuangding Hospital of Qingdao University, Yantai, China.
authors.forename: Xicheng
authors.initials: X
authors.lastname: Song
authors.affiliation: Department of Head and Neck Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.|Department of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
authors.forename: Guojun
authors.initials: G
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation; D003131:Combined Modality Therapy; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000401:mortality / Q000473:pathology; D008297:Male; D008875:Middle Aged; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D061105:Peroxiredoxin III / Q000235:genetics* / Q000378:metabolism
chemical_list: C516115:PRDX3 protein, human; D061105:Peroxiredoxin III
keywords: Hep-2 cell line; laryngeal squamous cell carcinoma; oxidative stress; peroxiredoxin 3