title: A report on extensive lateral genetic reciprocation between arsenic resistant Bacillus subtilis and Bacillus pumilus strains analyzed using RAPD-PCR.
abstract: The study involves isolation of arsenic resistant bacteria from soil samples. The characterization of bacteria isolates was based on 16S rRNA gene sequences. The phylogenetic consanguinity among isolates was studied employing rpoB and gltX gene sequence. RAPD-PCR technique was used to analyze genetic similarity between arsenic resistant isolates. In accordance with the results Bacillus subtilis and Bacillus pumilus strains may exhibit extensive horizontal gene transfer. Arsenic resistant potency in Bacillus sonorensis and high arsenite tolerance in Bacillus pumilus strains was identified. The RAPD-PCR primer OPO-02 amplified a 0.5kb DNA band specific to B. pumilus 3ZZZ strain and 0.75kb DNA band specific to B. subtilis 3PP. These unique DNA bands may have potential use as SCAR (Sequenced Characterized Amplified Region) molecular markers for identification of arsenic resistant B. pumilus and B. subtilis strains.
authors.affiliation: Department of Biotechnology, Faculty of Science, Hamdard University (Jamia Hamdard), New Delhi 110 062, India.
authors.forename: Sapna
authors.initials: S
authors.lastname: Khowal
authors.affiliation: Department of Biotechnology, Faculty of Science, Hamdard University (Jamia Hamdard), New Delhi 110 062, India.
authors.forename: Md Zulquarnain
authors.initials: MZ
authors.lastname: Siddiqui
authors.affiliation: Department of Biotechnology, Faculty of Science, Hamdard University (Jamia Hamdard), New Delhi 110 062, India.
authors.forename: Shadab
authors.initials: S
authors.lastname: Ali
authors.affiliation: Department of Biotechnology, Faculty of Science, Hamdard University (Jamia Hamdard), New Delhi 110 062, India.
authors.forename: Mohd Taha
authors.initials: MT
authors.lastname: Khan
authors.affiliation: 247, Bond Life Sciences Centre, 1201 Rollins Street, University of Missouri-Columbia, Columbia, MO 65211, USA.
authors.forename: Mather Ali
authors.initials: MA
authors.lastname: Khan
authors.affiliation: Genetix Biotech Asia (P) Ltd., New Delhi 110 015, India.
authors.forename: Samar Husain
authors.initials: SH
authors.lastname: Naqvi
authors.affiliation: Department of Biotechnology, Faculty of Science, Hamdard University (Jamia Hamdard), New Delhi 110 062, India. Electronic address: swajid@jamiahamdard.ac.in.
authors.forename: Saima
authors.initials: S
authors.lastname: Wajid
mesh_terms: D001151:Arsenic / Q000633:toxicity*; D000069979:Bacillus pumilus / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D001412:Bacillus subtilis / Q000187:drug effects* / Q000235:genetics* / Q000302:isolation & purification; D019143:Evolution, Molecular; D005798:Genes, Bacterial; D006863:Hydrogen-Ion Concentration; D016013:Likelihood Functions; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000379:methods*; D019105:Random Amplified Polymorphic DNA Technique / Q000379:methods*; D012987:Soil
chemical_list: D012987:Soil; D001151:Arsenic
keywords: Bacillus; Horizontal gene transfer; RAPD-PCR; SCAR molecular markers