title: Development of Lateral Flow Assay Based on Size-Controlled Gold Nanoparticles for Detection of Hepatitis B Surface Antigen.
abstract: In this study, we developed lateral flow assay (LFA) biosensors for the detection of hepatitis B surface antigens using well-controlled gold nanoparticles (AuNPs). To enhance colorimetric signals, a seeded growth method was used for the preparation of size-controlled AuNPs with a narrow size distribution. Different sizes of AuNPs in the range of 342-137.8 nm were conjugated with antibodies and then optimized for the efficient detection of LFA biosensors. The conjugation stability was investigated by UV-vis spectroscopy of AuNP dispersion at various pH values and concentrations of antibody. Based on optimized conjugation conditions, the use of 42.7 ± 0.8 nm AuNPs exhibited superior performance for the detection of LFAs relative to other sizes of AuNPs.
authors.affiliation: Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea. nyongtory91@gmail.com.
authors.forename: Dong Seok
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea. ytkim@nnfc.re.kr.
authors.forename: Yong Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea. skychojja@gmail.com.
authors.forename: Seok Bok
authors.initials: SB
authors.lastname: Hong
authors.affiliation: Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea. anlfkrn2000@gmail.com.
authors.forename: Jinwoon
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea. bioheo6@gmail.com.
authors.forename: Nam Su
authors.initials: NS
authors.lastname: Huh
authors.affiliation: Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea. mklee@nnfc.re.kr.
authors.forename: Moon-Keun
authors.initials: MK
authors.lastname: Lee
authors.affiliation: Nanobio Application Team, National NanoFab Center (NNFC), Daejeon 34141, Korea. sjlee@nnfc.re.kr.
authors.forename: Seok Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Research and Development Center, Tomorrow & Solution (T&S), E19-604, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea. kbiset@gmail.com.
authors.forename: Byeong Il
authors.initials: BI
authors.lastname: Kim
authors.affiliation: Research Center 2, Eudipia Inc., Cheongju 361-951, Korea. i.s.kim@eudipia.com.
authors.forename: In Soo
authors.initials: IS
authors.lastname: Kim
authors.affiliation: Department of Biological Engineering, Inha University, 100 Inha-ro, Nam-gu, Incheon 22212, Korea. yunsuk.huh@inha.ac.kr.
authors.forename: Yun Suk
authors.initials: YS
authors.lastname: Huh
authors.affiliation: Department of Chemical Engineering, Kangwon National University, Samcheok 25913, Korea. bgchoi@kangwon.ac.kr.
authors.forename: Bong Gill
authors.initials: BG
authors.lastname: Choi
mesh_terms: D003116:Color; D006046:Gold / Q000737:chemistry*; D006514:Hepatitis B Surface Antigens / Q000032:analysis*; D006801:Humans; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010316:Particle Size; D011933:Reagent Kits, Diagnostic; D012212:Rheology / Q000379:methods*; D013056:Spectrophotometry, Ultraviolet
chemical_list: D006514:Hepatitis B Surface Antigens; D011933:Reagent Kits, Diagnostic; D006046:Gold
keywords: conjugation; gold; hepatitis B surface antigen; lateral flow assay; nanoparticle