title: Efficacy of α-Copaene, Cubeb, and Eucalyptol Lures for Detection of Redbay Ambrosia Beetle (Coleoptera: Curculionidae: Scolytinae).
abstract: Redbay ambrosia beetle, Xyleborus glabratus Eichhoff, is a wood-boring pest that has now invaded nine states in the southeastern United States. The beetle's dominant fungal symbiont (Raffaelea lauricola) is phytopathogenic, inducing laurel wilt in trees within the family Lauraceae. Members of the genus Persea are particularly susceptible to the lethal disease, including native redbay (P. borbonia) and swampbay (P. palustris), as well as commercial avocado (P. americana). Cubeb oil lures are the current standard for detection of X. glabratus, but recently eucalyptol and a 50% α-copaene oil have been identified as additional attractants. This study used a combination of binary-choice bioassays, field cage release-and-recapture assays, and a 12-wk field trial to compare efficacy of eucalyptol and copaene lures relative to commercial cubeb lures. In addition, GC-MS was used to quantify emissions from lures field-aged for 12 wk. In laboratory bioassays, copaene lures were more attractive than eucalyptol lures. In field cage assays, copaene lures recaptured a higher percentage of released beetles than cubeb lures. In the field test, cubeb lures captured fewer beetles than copaene lures, and lowest captures were obtained with eucalyptol lures. Combining eucalyptol with either copaene or cubeb lures did not increase captures over those lures deployed alone. Both copaene and cubeb lures were effective in attracting X. glabratus for 12 wk, but field life of eucalyptol lures was only 4 wk, consistent with the quantification of lure emissions. Results suggest that the 50% α-copaene lure provides the best pest detection currently available for X. glabratus.
authors.affiliation: USDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Rd., Miami, FL 33158 (paul.kendra@ars.usda.gov; wayne.montgomery@ars.usda.gov; elena.schnell@ars.usda.gov; Nancy.Epsky@ars.usda.gov) paul.kendra@ars.usda.gov.
authors.forename: Paul E
authors.initials: PE
authors.lastname: Kendra
authors.affiliation: USDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Rd., Miami, FL 33158 (paul.kendra@ars.usda.gov; wayne.montgomery@ars.usda.gov; elena.schnell@ars.usda.gov; Nancy.Epsky@ars.usda.gov).
authors.forename: Wayne S
authors.initials: WS
authors.lastname: Montgomery
authors.affiliation: USDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Rd., Miami, FL 33158 (paul.kendra@ars.usda.gov; wayne.montgomery@ars.usda.gov; elena.schnell@ars.usda.gov; Nancy.Epsky@ars.usda.gov).
authors.forename: Elena Q
authors.initials: EQ
authors.lastname: Schnell
authors.affiliation: Archbold Biological Station, P.O. Box 2057, Lake Placid, FL 33862 (mdeyrup@archbold-station.org).
authors.forename: Mark A
authors.initials: MA
authors.lastname: Deyrup
authors.affiliation: USDA-ARS, Subtropical Horticulture Research Station, 13601 Old Cutler Rd., Miami, FL 33158 (paul.kendra@ars.usda.gov; wayne.montgomery@ars.usda.gov; elena.schnell@ars.usda.gov; Nancy.Epsky@ars.usda.gov).
authors.forename: Nancy D
authors.initials: ND
authors.lastname: Epsky
mesh_terms: D000818:Animals; D003511:Cyclohexanols / Q000494:pharmacology*; D000077591:Eucalyptol; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007300:Insect Control; D039821:Monoterpenes / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology; D027421:Persea / Q000254:growth & development; D031701:Piper / Q000737:chemistry; D010938:Plant Oils / Q000494:pharmacology*; D012717:Sesquiterpenes / Q000494:pharmacology*; D048889:Weevils / Q000187:drug effects* / Q000502:physiology
chemical_list: D003511:Cyclohexanols; D039821:Monoterpenes; D009822:Oils, Volatile; D010938:Plant Oils; D012717:Sesquiterpenes; C000599751:alpha-copaene; D000077591:Eucalyptol
keywords: Lauraceae; Xyleborus glabratus; kairomone; laurel wilt; sesquiterpene