title: Two-Dimensional Electrophoresis Protocols to Analyze the Microtubule-Associated Tau Proteins from Several Biological Sources.
abstract: Technology breakthrough in proteomics enables to gather qualitative and quantitative information about a protein or a complex mixture of proteins. Two-dimensional gel electrophoresis remains an interesting technique, which provides an overview of the complexity of isovariants from a single protein when coupled to western blotting. Here, we describe a detailed protocol for the two-dimensional analysis of microtubule-associated Tau isovariants from cell to human or mouse brain tissue. We provide protocol enabling to separate native and Tau proteins that are aggregated in neurodegenerative disorders such as Alzheimer's disease.
authors.affiliation: Inserm UMR-S1172, Jean-Pierre Aubert Centre, Alzheimer & Tauopathies Team 1, 1 Place de Verdun, 59045, Lille, France. nicolas.sergeant@inserm.fr.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Sergeant
authors.affiliation: Inserm UMR-S1172, Jean-Pierre Aubert Centre, Alzheimer & Tauopathies Team 1, 1 Place de Verdun, 59045, Lille, France.
authors.forename: Francisco-Jose
authors.initials: FJ
authors.lastname: Fernandez-Gomez
authors.affiliation: Inserm UMR-S1172, Jean-Pierre Aubert Centre, Alzheimer & Tauopathies Team 1, 1 Place de Verdun, 59045, Lille, France.
authors.forename: Helene
authors.initials: H
authors.lastname: Obriot
authors.affiliation: Inserm UMR-S1172, Jean-Pierre Aubert Centre, Alzheimer & Tauopathies Team 1, 1 Place de Verdun, 59045, Lille, France.
authors.forename: Sabiha
authors.initials: S
authors.lastname: Eddarkaoui
authors.affiliation: Inserm UMR-S1172, Jean-Pierre Aubert Centre, Alzheimer & Tauopathies Team 1, 1 Place de Verdun, 59045, Lille, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Buée-Scherrer
authors.affiliation: Inserm UMR-S1172, Jean-Pierre Aubert Centre, Alzheimer & Tauopathies Team 1, 1 Place de Verdun, 59045, Lille, France.
authors.forename: Luc
authors.initials: L
authors.lastname: Buée
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism; D000818:Animals; D001921:Brain / Q000378:metabolism; D015180:Electrophoresis, Gel, Two-Dimensional / Q000379:methods*; D006801:Humans; D007525:Isoelectric Focusing; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000737:chemistry / Q000378:metabolism*; D011499:Protein Processing, Post-Translational; D016875:tau Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D008869:Microtubule-Associated Proteins; D016875:tau Proteins
keywords: Alzheimer’s disease; Brain tissue; Isoelecfocusing; Posttranslational modifications; Tau protein isovariants; Two-dimensional gel electrophoresis