title: Antibiotic susceptibility of Legionella strains isolated from public water sources in Macau and Guangzhou.
abstract: The purpose of this study was to investigate the susceptibility of waterborne strains of Legionella to eight antimicrobials commonly used in legionellosis therapy. The minimum inhibitory concentrations (MICs) of 66 environmental Legionella strains, isolated from fountains and cooling towers of public facilities (hotels, schools, and shopping malls) in Macau and Guangzhou, were tested using the microdilution method in buffered yeast extract broth. The MIC50/MIC90values for erythromycin, cefotaxime (CTX), doxycycline (DOC), minocycline (MIN), azithromycin, ciprofloxacin, levofloxacin (LEV), and moxifloxacin were 0.125/0.5 mg/L, 4/8 mg/L, 8/16 mg/L, 4/8 mg/L, 0.125/0.5 mg/L, 0.031/0.031 mg/L, 0.031/0.031 mg/L, and 0.031/0.062 mg/L, respectively. Legionella isolates were inhibited by either low concentrations of macrolides and fluoroquinolones, or high concentrations of CTX and tetracycline drugs. LEV was the most effective drug against different Legionella species and serogroups of L. pneumophila isolates. The latter were inhibited in decreasing order by MIN > CTX >DOC, while non-L. pneumophila isolates were inhibited by CTX> MIN >DOC. In this study, we evaluated drug resistance of pathogenic bacteria from the environment. This may help predict the emergence of drug resistance, improve patient outcomes, and reduce hospitalization costs.
authors.affiliation: School of Food Science and Technology, South China University of Technology, No. 381, Wushan Road, TianHe District, Guangzhou, Guangdong 510641, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Xiong
authors.affiliation: School of Food Science and Technology, South China University of Technology, No. 381, Wushan Road, TianHe District, Guangzhou, Guangdong 510641, China.
authors.forename: He
authors.initials: H
authors.lastname: Yan
authors.affiliation: School of Food Science and Technology, South China University of Technology, No. 381, Wushan Road, TianHe District, Guangzhou, Guangdong 510641, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Respiratory Disease, No. 151, YanJiang Xi Road, YueXiu District, Guangzhou, Guangdong 510120, China E-mail: moziyao@gird.cn.
authors.forename: Ziyao
authors.initials: Z
authors.lastname: Mo
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002681:China; D060766:Drinking Water / Q000382:microbiology*; D024881:Drug Resistance, Bacterial; D007875:Legionella / Q000187:drug effects*; D016952:Legionella pneumophila / Q000187:drug effects; D008256:Macau; D008826:Microbial Sensitivity Tests
chemical_list: D000900:Anti-Bacterial Agents; D060766:Drinking Water