title: Confirmation of β-agonist residues in bovine retina and liver using HPLC-MS/MS and evaluation of matrix-dependent problems.
abstract: β-Agonists or β-adrenergic agonists are synthetic drugs sometimes used for therapeutic purposes in veterinary medicine. However, these compounds also belong to a class of illegal growth promoters that decrease the fat content in farm animals. The purpose of this study was to develop an analytical LC-MS/MS confirmatory method for the detection of nine β-agonists in bovine retina and liver tissues. The obtained CCα values ranged from 0.29 to 1.54 µg kg-1in retina and from 0.03 to 0.18 µg kg-1in liver, and CCβ values from 0.50 to 2.63 µg kg-1and from 0.05 to 0.30 µg kg-1, respectively. Additionally, the importance of a matrix effect in these two tissues for the achievement of accurate detection of non-compliant samples was studied and discussed. It was concluded that the main characteristic of this phenomena is its considerable variability from one animal to another. Also, the total amount of matrix in the final extract can provide differences in the matrix-matched calibration line.
authors.affiliation: a Department of Analytical Chemistry, Nutrition and Bromatology , University of Santiago de Compostela , Lugo , Spain.
authors.forename: Patricia
authors.initials: P
authors.lastname: Regal
authors.affiliation: a Department of Analytical Chemistry, Nutrition and Bromatology , University of Santiago de Compostela , Lugo , Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Díaz-Bao
authors.affiliation: a Department of Analytical Chemistry, Nutrition and Bromatology , University of Santiago de Compostela , Lugo , Spain.
authors.forename: Rocío
authors.initials: R
authors.lastname: Barreiro
authors.affiliation: a Department of Analytical Chemistry, Nutrition and Bromatology , University of Santiago de Compostela , Lugo , Spain.
authors.forename: Beatriz I
authors.initials: BI
authors.lastname: Vázquez
authors.affiliation: a Department of Analytical Chemistry, Nutrition and Bromatology , University of Santiago de Compostela , Lugo , Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Cepeda
mesh_terms: D000318:Adrenergic beta-Agonists / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid / Q000379:methods / Q000592:standards; D004350:Drug Residues / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D008099:Liver / Q000737:chemistry* / Q000187:drug effects; D008460:Meat / Q000032:analysis*; D009928:Organ Specificity; D012160:Retina / Q000737:chemistry* / Q000187:drug effects; D052616:Solid Phase Extraction / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods / Q000592:standards; D019155:Veterinary Drugs / Q000032:analysis* / Q000378:metabolism / Q000493:pharmacokinetics
chemical_list: D000318:Adrenergic beta-Agonists; D019155:Veterinary Drugs
keywords: HPLC-MS/MS; bovine; liver; matrix effect; retina; β-Agonists