title: Skin-derived precursors possess the ability of differentiation into the epidermal progeny and accelerate burn wound healing.
abstract: Skin-derived precursors (SKPs) are remnants of the embryonic neural crest stem cells that reside in the dermis until adulthood. Although they possess a wide range of differentiation potentials, their differentiation into keratinocyte-like cells and their roles in skin wound healing are obscure. The present study aimed to investigate the differentiation of SKPs into keratinocyte-like cells and evaluate their role in healing of third degree burn wounds. To this aim, SKPs were differentiated into keratinocyte-like cells on tissue culture plate and collagen-chitosan scaffold prepared by freeze-drying. Their differentiation capability was detected by real-time RT-PCR and immunofluorescence. Thereafter, they were cultured on scaffold and implanted in a rat model of burn wound. Histopathological and immunohistochemical analyses were employed to examine the reconstituted skin. The research findings revealed that SKPs were able to differentiate along the epidermal lineage and this ability can be enhanced on a suitable scaffold. Additionally, the results indicated that SKPs apparently promoted wound healing process and accelerate its transition from proliferating stage to maturational phase, especially if they were differentiated into keratinocyte-like cells. Regarding the results, it is concluded that SKPs are able to differentiate into keratinocyte-like cells, particularly when they are cultured on collagen-chitosan scaffold. Moreover, they can regenerate epidermal and dermal layers including thick collagen bundles, possibly through differentiation into keratinocyte-like cells.
authors.affiliation: Cellular and Molecular Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 61357-15794, Iran.|Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 61357-15794, Iran.
authors.forename: Vahid
authors.initials: V
authors.lastname: Bayati
authors.affiliation: Targeted Drug Delivery Research Centre, Mashhad University of Medical Sciences, Mashhad, 91775-1365, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Abbaspour
authors.affiliation: Department of Medical Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 61357-15794, Iran.
authors.forename: Niloofar
authors.initials: N
authors.lastname: Neisi
authors.affiliation: Cellular and Molecular Research Centre, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 61357-15794, Iran.|Department of Anatomical Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, 61357-15794, Iran.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Hashemitabar
mesh_terms: D000818:Animals; D002056:Burns / Q000473:pathology* / Q000628:therapy; D002454:Cell Differentiation; D019070:Cell Lineage; D002478:Cells, Cultured; D000078404:Epidermal Cells; D005353:Fibronectins / Q000378:metabolism; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins / Q000235:genetics / Q000378:metabolism; D053547:Keratin-14 / Q000235:genetics / Q000378:metabolism; D015603:Keratinocytes / Q000166:cytology / Q000378:metabolism; D011498:Protein Precursors / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D044968:Regenerative Medicine; D012867:Skin / Q000166:cytology* / Q000473:pathology; D013234:Stem Cells / Q000166:cytology* / Q000378:metabolism; D054457:Tissue Scaffolds / Q000737:chemistry; D014746:Vimentin / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology*
chemical_list: D005353:Fibronectins; D000091344:Filaggrin Proteins; D007381:Intermediate Filament Proteins; D053547:Keratin-14; D011498:Protein Precursors; D014746:Vimentin; C032253:involucrin
keywords: differentiation; keratinocyte-like cells; skin-derived precursors; wound