title: Glycated hemoglobin biosensing integration formed on Au nanoparticle-dotted tubular TiO2nanoarray.
abstract: Excessive glucose present in the blood of diabetic patients binds with the hemoglobin of red blood cells resulting in the formation of glycated hemoglobin (HbA1c). Measurement of HbA1clevels may help in identifying the efficacy of the ongoing treatment and hence provide a better control over the disease. In the present study, we have synthesized a sensitive and stable scaffold, which consists of Au nanoparticles (GNPs)-dotted tubular TiO2, for the construction of an electrochemical HbA1cbiosensor. 12-phosphotungstic acid has been used as a reducer after depositing well-dispersed GNPs on TiO2nanotubes (TiO2NTs) and an electron mediator by accelerating the electron transfer between the conductor and protein. The fabricated electrode was characterized using scanning electron microscopy (SEM), cyclic voltammetry (CV), Fourier transform infrared spectroscopy (FTIR) and electrochemical impedance spectroscopic analysis (EIS). Biosensor exhibited low detection limit (0.5 μM), fast response time (3 s) and wide linearity (from 0.5 to 2000 μM). The working electrode was used 100 times over 4 months, when stored at 4 °C. The HbA1c biosensor was then effectively used to measure the % of HbA1cin the blood of apparently healthy persons and diabetic patients.
authors.affiliation: Amity Institute of Nanotechnology, Amity University, Noida, 201303, Uttar Pradesh, India.
authors.forename: Utkarsh
authors.initials: U
authors.lastname: Jain
authors.affiliation: Department of Biotechnology, UIET, Kurukshetra University, Kurukshetra, 136 119, Haryana, India.
authors.forename: Anamika
authors.initials: A
authors.lastname: Singh
authors.affiliation: Clinical Biochemistry Department, Bio-Diagnostics, Rohini, Delhi, 110085, India.
authors.forename: Naresh Kumar
authors.initials: NK
authors.lastname: Kuchhal
authors.affiliation: Amity Institute of Nanotechnology, Amity University, Noida, 201303, Uttar Pradesh, India. Electronic address: nidhichauhan2007@rediffmail.com.
authors.forename: Nidhi
authors.initials: N
authors.lastname: Chauhan
mesh_terms: D015374:Biosensing Techniques; D006442:Glycated Hemoglobin / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D017550:Spectroscopy, Fourier Transform Infrared; D014025:Titanium / Q000737:chemistry*
chemical_list: D006442:Glycated Hemoglobin; D006046:Gold; D014025:Titanium; C517652:hemoglobin A1c protein, human; C009495:titanium dioxide
keywords: Glycated hemoglobin; Gold nanoparticle; ITO electrode; TiO(2) nanotubes