title: MiR-200a acts as an oncogene in colorectal carcinoma by targeting PTEN.
abstract: The expression pattern of miR-200a in different types of cancer is diverse, and its mechanism in tumorigenesis has yet to be elucidated. In this study, miR-200a was significantly upregulated in the cancer tumor tissues of colorectal cancer (CRC) patients, and its expression was positively correlated with the degree of tumor differentiation. Overexpression of miR-200a enhanced cell proliferation, migration, and invasion. To understand the potential mechanism of miR-200a in tumorigenesis, we showed that miR-200a directly targeted phosphatase and tensin homolog (PTEN). To test the clinical relevance of these results, we used 107 pairs of CRC and adjacent normal tissues, analyzed miR-200a levels and PTEN expression in these tissues, and found that miR-200a levels were significantly inversely correlated with PTEN levels in the cancer tissues. These results suggest that miR-200a plays an oncogene role by regulating PTEN signaling in CRC. Our findings present important implications for further understanding the signaling mechanisms involved in modulating CRC tumorigenesis.
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Jinglu
authors.initials: J
authors.lastname: Sun
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Department of Pathology, Anhui Medical College, Hefei 230601, Anhui Province, China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China. Electronic address: aydyinyu@aliyun.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, Anhui Province, China. Electronic address: Lihao3402@aliyun.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Li
mesh_terms: D002277:Carcinoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D051059:PTEN Phosphohydrolase / Q000235:genetics* / Q000378:metabolism
chemical_list: C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human
keywords: Colorectal cancer; Immunohistochemistry; In situ hybridization; PTEN; miR-200a