title: Nanochannel Electroporation as a Platform for Living Cell Interrogation in Acute Myeloid Leukemia.
abstract: A living cell interrogation platform based on nanochannel electroporationis demonstrated with analysis of RNAs in single cells. This minimally invasive process is based on individual cells and allows both multi-target analysis and stimulus-response analysis by sequential deliveries. The unique platform possesses a great potential to the comprehensive and lysis-free nucleic acid analysis on rare or hard-to-transfect cells.
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA; William G. Lowrie, Department of Chemical and Biomolecular Engineering The Ohio State University Columbus OH 43210 USA.
authors.forename: Xi
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
authors.forename: Xiaomeng
authors.initials: X
authors.lastname: Huang
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
authors.forename: Xinmei
authors.initials: X
authors.lastname: Wang
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
authors.forename: Yun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Internal Medicine Comprehensive Cancer Center The Ohio State University Columbus OH 43210 USA.
authors.forename: Ann-Kathrin
authors.initials: AK
authors.lastname: Eisfeld
authors.affiliation: Department of Internal Medicine Comprehensive Cancer Center The Ohio State University Columbus OH 43210 USA.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schwind
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Gallego-Perez
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA.
authors.forename: Pouyan E
authors.initials: PE
authors.lastname: Boukany
authors.affiliation: Department of Internal Medicine Comprehensive Cancer Center The Ohio State University Columbus OH 43210 USA.
authors.forename: Guido I
authors.initials: GI
authors.lastname: Marcucci
authors.affiliation: Center for Affordable Nanoengineering of Polymeric Biomedical Devices The Ohio State University Columbus OH 43212 USA; William G. Lowrie, Department of Chemical and Biomolecular Engineering The Ohio State University Columbus OH 43210 USA.
authors.forename: Ly James
authors.initials: LJ
authors.lastname: Lee
keywords: RNA recognition; fluorescent probes; living cell interrogation; nanochannel electroporation