title: HPTLC Fingerprinting and Cholinesterase Inhibitory and Metal-Chelating Capacity of VariousCitrusCultivars andOlea europaea.
abstract: Inhibitory activity of thirty-one ethanol extracts obtained from albedo, flavedo, seed and leaf parts of 17 cultivars ofCitrusspecies from Turkey, the bark and leaves ofOlea europaeaL. from two locations (Turkey and Cyprus) as well as caffeic acid and hesperidin was tested against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), related to the pathogenesis of Alzheimer's disease, using ELISA microtiter assays at 500 µg/mL. Metal-chelating capacity of the extracts was also determined. BChE inhibitory effect of theCitrussp. extracts was from (7.7±0.7) to (70.3±1.1) %, whereas they did not show any inhibition against AChE. Cholinesterase inhibitory activity of the leaf and bark ethanol extracts ofO. europaeawas very weak ((10.2±3.1) to (15.0±2.3) %). The extracts had either no or low metal-chelating capacity at 500 µg/mL. HPTLC fingerprinting of the extracts, which indicated a similar phytochemical pattern, was also done using the standards of caffeic acid and hesperidin with weak cholinesterase inhibition. Among the screened extracts, the albedo extract ofC. limon'Interdonato', the flavedo extracts of 'Kara Limon' and 'Cyprus' cultivars and the seed extract ofC. maximaappear to be promising as natural BChE inhibitors.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, TR-06330 Ankara, Turkey.
authors.forename: Fatma Sezer
authors.initials: FS
authors.lastname: Senol
authors.affiliation: CAMAG Laboratory, Sonnenmattstrasse 11, CH-4132 Muttenz, Switzerland.
authors.forename: Anita
authors.initials: A
authors.lastname: Ankli
authors.affiliation: CAMAG Laboratory, Sonnenmattstrasse 11, CH-4132 Muttenz, Switzerland.
authors.forename: Eike
authors.initials: E
authors.lastname: Reich
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, TR-06330 Ankara, Turkey.
authors.forename: Ilkay Erdogan
authors.initials: IE
authors.lastname: Orhan
keywords: Citrus sp.; HPTLC; Olea europaea; cholinesterase inhibition; metal-chelating activity