title: RNA sequencing analysis of the developing chicken retina.
abstract: RNA sequencing transcriptome analysis using massively parallel next generation sequencing technology provides the capability to understand global changes in gene expression throughout a range of tissue samples. Development of the vertebrate retina requires complex temporal orchestration of transcriptional activation and repression. The chicken embryo (Gallus gallus) is a classic model system for studying developmental biology and retinogenesis. Existing retinal transcriptome projects have been critical to the vision research community for studying aspects of murine and human retinogenesis, however, there are currently no publicly available data sets describing the developing chicken retinal transcriptome. Here we used Illumina RNA sequencing (RNA-seq) analysis to characterize the mRNA transcriptome of the developing chicken retina in an effort to identify genes critical for retinal development in this important model organism. These data will be valuable to the vision research community for characterizing global changes in gene expression between ocular tissues and critical developmental time points during retinogenesis in the chicken retina.
authors.affiliation: Department of Biology, James Madison University, Harrisonburg, Virginia 22807, USA.
authors.forename: Christophe J
authors.initials: CJ
authors.lastname: Langouet-Astrie
authors.affiliation: Department of Biology, James Madison University, Harrisonburg, Virginia 22807, USA.
authors.forename: Annamarie L
authors.initials: AL
authors.lastname: Meinsen
authors.affiliation: Department of Biology, James Madison University, Harrisonburg, Virginia 22807, USA.
authors.forename: Emily R
authors.initials: ER
authors.lastname: Grunwald
authors.affiliation: Department of Public Health Sciences University of Virginia, Charlottesville, Virginia 22908, USA.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Turner
authors.affiliation: Department of Biology, James Madison University, Harrisonburg, Virginia 22807, USA.|Center for Genome &Metagenome Studies, James Madison University, Harrisonburg, Virginia 22807, USA.
authors.forename: Raymond A
authors.initials: RA
authors.lastname: Enke
mesh_terms: D000818:Animals; D002642:Chick Embryo; D012333:RNA, Messenger / Q000235:genetics; D012160:Retina / Q000196:embryology* / Q000378:metabolism; D017423:Sequence Analysis, RNA; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger