title: The common dietary flavonoid myricetin attenuates liver fibrosis in carbon tetrachloride treated mice.
abstract: Myricetin is found in most berries, vegetables, and various medicinal herbs, which has been reported to possess various bio-activities. However, the role of myricetin on liver fibrosis remains to be elucidated.
  
  
  
  Hepatic stellate cell (HSC) line CFSC-8B was stimulated by transforming growth factor β1 (TGF-β1) or platelet-derived growth factor BB (PDGF-BB) to induce liver fibrosis in vitro. The results showed that myricetin significantly ameliorated TGF-β1- or PDGF-BB-induced HSCs activation, cell migration, and extracellular matrix production; blocked TGF-β1-induced phosphorylation of Smad2, P38, extracellular signal-regulated kinase (ERK), and protein kinase B (Akt); and downregulated PDGF-BB stimulated phosphorylation of extracellular signal-regulated kinase and Akt in HSCs in a dose-dependent manner. Meanwhile, the carbon tetrachloride (CCl4) induced mouse model has been used to study antifibrosis role of myricetin in vivo. Our data demonstrated that myricetin suppressed α-smooth muscle actin and collagen type I deposition and blocked phosphorylation of Smad2, mitogen-activated protein kinases, and Akt in CCl4-treated mice.
  
  
  
  Myricetin inhibits the activation of HSCs and ameliorates CCl4-induced liver fibrosis in mice and may serve as a potential therapeutic agent in the treatment of liver fibrosis.
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Geng
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Sun
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Zhen-Ming
authors.initials: ZM
authors.lastname: Lu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Xu
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, China.
authors.forename: Jin-Song
authors.initials: JS
authors.lastname: Shi
authors.affiliation: School of Pharmaceutical Science, Jiangnan University, Wuxi, Jiangsu, China.|Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
authors.forename: Zheng-Hong
authors.initials: ZH
authors.lastname: Xu
mesh_terms: D000818:Animals; D000933:Antifibrinolytic Agents / Q000378:metabolism; D000077214:Becaplermin; D002251:Carbon Tetrachloride / Q000009:adverse effects*; D002465:Cell Movement / Q000187:drug effects; D024042:Collagen Type I / Q000378:metabolism; D004195:Disease Models, Animal; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005419:Flavonoids / Q000494:pharmacology*; D055166:Hepatic Stellate Cells / Q000378:metabolism; D008103:Liver Cirrhosis / Q000139:chemically induced / Q000188:drug therapy; D051379:Mice; D010766:Phosphorylation; D010982:Platelet-Derived Growth Factor / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism; D020574:Proto-Oncogene Proteins c-sis / Q000494:pharmacology; D015398:Signal Transduction / Q000187:drug effects; D016212:Transforming Growth Factor beta / Q000378:metabolism; D053773:Transforming Growth Factor beta1 / Q000378:metabolism*
chemical_list: D000933:Antifibrinolytic Agents; D024042:Collagen Type I; D005419:Flavonoids; D010982:Platelet-Derived Growth Factor; D020574:Proto-Oncogene Proteins c-sis; D016212:Transforming Growth Factor beta; D053773:Transforming Growth Factor beta1; D000077214:Becaplermin; C040015:myricetin; D002251:Carbon Tetrachloride; D051057:Proto-Oncogene Proteins c-akt; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: Antifibrotic activity; Liver fibrosis; Myricetin; Platelet-derived growth factor (PDGF); Transforming growth factor β1 (TGF-β1)