title: Isolation of PCR ready-human DNA using copper nanoparticles from skeletal remains.
abstract: Present study represents a novel approach of PCR ready-human DNA extraction method from skeletal remains using copper nanoparticles (CuNPs) for personnel identification. To achieve rapid, cost effective, sensitive and non-hazardous method for DNA extraction we utilized CuNPs synthesized using microwave. The applicability of this approach was first tested in blood samples and afterwards, this system was extended to skeletal remains' samples also. This method yields good quality DNA that are ready for PCR reactions from small quantities of blood and skeletal remains. Consequently, even small quantities of nanoparticles could be potentially utilized for a highly efficient isolation of DNA from skeletal remains as well as from ancient archaeological samples. The present method has the advantages that it is quick with high yield, inexpensive, robust, environment friendly and does not require use of hazardous organic solvents.
authors.affiliation: Department of Forensic Science, Gujarat University, Ahmedabad 380009, India. Electronic address: forensicwithanand@gmail.com.
authors.forename: Anand
authors.initials: A
authors.lastname: Lodha
authors.affiliation: Department of Forensic Science, Gujarat University, Ahmedabad 380009, India. Electronic address: n.ansari3@gmail.com.
authors.forename: Niha
authors.initials: N
authors.lastname: Ansari
authors.affiliation: Department of Zoology, Gujarat University, Ahmedabad 380009, India.
authors.forename: Shahil
authors.initials: S
authors.lastname: Shah
authors.affiliation: Department of Zoology, Gujarat University, Ahmedabad 380009, India.
authors.forename: M V
authors.initials: MV
authors.lastname: Rao
authors.affiliation: Department of Forensic Science, Gujarat University, Ahmedabad 380009, India. Electronic address: shobhanamenon07@gmail.com.
authors.forename: Shobhana K
authors.initials: SK
authors.lastname: Menon
mesh_terms: D000071298:Body Remains; D001842:Bone and Bones / Q000737:chemistry*; D003300:Copper; D004247:DNA / Q000302:isolation & purification*; D016172:DNA Fingerprinting; D004587:Electrophoresis, Agar Gel; D006801:Humans; D053768:Metal Nanoparticles; D016133:Polymerase Chain Reaction
chemical_list: D003300:Copper; D004247:DNA
keywords: Blood; Copper nanoparticles; DNA extraction; Forensic science; Polymerase chain reaction; Skeletal remains