title: Analysis of tilianin and acacetin inAgastache rugosaby high-performance liquid chromatography with ionic liquids-ultrasound based extraction.
abstract: Ionic liquid 1-butyl-3-methylimidazolium bromide-methanol-based ultrasonic-assisted extraction (ILUAE) was used to extract tilianin and acacetin from the aerial parts ofAgastache rugose(A. rugose), and simultaneously determined by reversed phase high performance liquid chromatographic (RP-HPLC) method with ultraviolet detection (RP-HPLC-UV). An InertSustain RP-C18column was used with the mobile phase consisting of methanol and 0.2% acetic acid as gradient elution at the detection wavelength of 332 nm. The flow rate was 0.8 mL/min, and the column temperature was 30 °C. Under the optimized conditions, tilianin and acacetin displayed good linearity in the ranges of 0.0595-4.76 and 0.0585-4.68 μg/mL, respectively, with the average recoveries being 96.93 and 97.88%, respectively. The method of ILUAE was compared with the traditional methods, it exhibited higher efficiency, higher reproducibility and environmental friendly in analyzing the active compounds in traditional Chinese medicines (TCMs).
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China ; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, 475004 China.
authors.forename: Jinfeng
authors.initials: J
authors.lastname: Wei
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.
authors.forename: Pengran
authors.initials: P
authors.lastname: Cao
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China.
authors.forename: Jinmei
authors.initials: J
authors.lastname: Wang
authors.affiliation: Institute of Chinese Materia Medica, Henan University, Kaifeng, 475004 China ; Kaifeng Key Laboratory of Functional Components in Health Food, Kaifeng, 475004 China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Kang
keywords: Acacetin; Agastache rugosa; HPLC; Ionic liquids-ultrasound; Tilianin