title: Different Vancomycin-Intermediate Staphylococcus aureus Phenotypes Selected from the Same ST100-hVISA Parental Strain.
abstract: The aim of this study is to characterize the factors related to peptidoglycan metabolism in isogenic hVISA/VISA ST100 strains. Recently, we reported the increase in IS256 transposition in invasive hVISA ST100 clinical strains isolated from the same patient (D1 and D2) before and after vancomycin treatment and two laboratory VISA mutants (D23C9 and D2P11) selected from D2 in independent experiments. High performance liquid chromatography-mass spectrometry (HPLC-MS) analysis of peptidoglycan muropeptides showed increased proportion of monomeric muropeptides and a concomitant decrease in the proportion of tetrameric muropeptide in D2 and derived mutants when compared to the original strain D1. In addition, strain D2 and its derived mutants showed an increase in cell wall thickness with increased pbp2 gene expression. The VISA phenotype was not stable in D2P11 and showed a reduced autolysis profile. On the other hand, the mutant D23C9 differentiates from D2 and D2P11 in the autolysis profile, and pbp4 transcription profile. D2-derived mutants exhibited differences in the susceptibility to other antimicrobials. Our results highlight the possibility of selection of different VISA phenotypes from a single hVISA-ST100 genetic background.
authors.affiliation: 1 Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Microbiología, Buenos Aires, Argentina .
authors.forename: Sabrina
authors.initials: S
authors.lastname: Di Gregorio
authors.affiliation: 1 Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Microbiología, Buenos Aires, Argentina .
authors.forename: Silvina
authors.initials: S
authors.lastname: Fernandez
authors.affiliation: 2 Unité de Physiologie et génétique bactériennes, Département de Sciences de la vie, Centre d'Ingénierie des Protéines, Université de Liège , Liège, Belgique.
authors.forename: Arabela
authors.initials: A
authors.lastname: Cuirolo
authors.affiliation: 2 Unité de Physiologie et génétique bactériennes, Département de Sciences de la vie, Centre d'Ingénierie des Protéines, Université de Liège , Liège, Belgique.
authors.forename: Olivier
authors.initials: O
authors.lastname: Verlaine
authors.affiliation: 2 Unité de Physiologie et génétique bactériennes, Département de Sciences de la vie, Centre d'Ingénierie des Protéines, Université de Liège , Liège, Belgique.
authors.forename: Ana
authors.initials: A
authors.lastname: Amoroso
authors.affiliation: 3 Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ Paris-Sud, University Paris-Saclay, Gif-sur-Yvette, France .
authors.forename: Dominique
authors.initials: D
authors.lastname: Mengin-Lecreulx
authors.affiliation: 4 Universidad de Buenos Aires, Hospital de Clínicas José de San Martín, Laboratorio de Bacteriología Clínica, Buenos Aires, Argentina .
authors.forename: Angela
authors.initials: A
authors.lastname: Famiglietti
authors.affiliation: 2 Unité de Physiologie et génétique bactériennes, Département de Sciences de la vie, Centre d'Ingénierie des Protéines, Université de Liège , Liège, Belgique.
authors.forename: Bernard
authors.initials: B
authors.lastname: Joris
authors.affiliation: 1 Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Departamento de Microbiología, Inmunología y Biotecnología, Cátedra de Microbiología, Buenos Aires, Argentina .
authors.forename: Marta
authors.initials: M
authors.lastname: Mollerach
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002473:Cell Wall / Q000737:chemistry / Q000187:drug effects / Q000378:metabolism; D004251:DNA Transposable Elements / Q000187:drug effects; D015870:Gene Expression; D006801:Humans; D008826:Microbial Sensitivity Tests; D009154:Mutation; D046915:Penicillin-Binding Proteins / Q000235:genetics / Q000378:metabolism; D010457:Peptidoglycan / Q000378:metabolism; D010641:Phenotype; D012641:Selection, Genetic; D013203:Staphylococcal Infections / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D014640:Vancomycin / Q000494:pharmacology*; D020713:Vancomycin Resistance / Q000235:genetics*
chemical_list: D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins; D004251:DNA Transposable Elements; D046915:Penicillin-Binding Proteins; D010457:Peptidoglycan; C487789:mecA protein, Staphylococcus aureus; D014640:Vancomycin
keywords: MRSA; ST100; Staphylococcus aureus; VISA; hVISA; vancomycin