title: A simple extraction method for the detection and quantification of polyoxin D, a nucleoside antibiotic, in butterbur using UPLC-MS/MS.
abstract: An effective analytical method was developed for the detection and quantification of polyoxin D in butterbur using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted using acidified deionised water and purified via solid-phase extraction (SPE) using an HLB cartridge. An external matrix-matched standard calibration curve was prepared, which provided an excellent linearity with a coefficient of determination (R2) ⩾0.999. Limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.0.05μg/g, respectively. The developed method was validated in terms of recovery performance using two fortification levels in triplicate. The storage stabilities of the various field samples were also determined. Our method provided consistent recovery (86.26-87.37%) with a relative standard deviation (RSD) of <5%, and was successfully applied to field-treated butterbur grown under greenhouse conditions and collected at various times following commercial fungicide application. As expected, a gradual degradation of polyoxin D was observed, with a half-life (t1/2) of 2.11d being recorded. Finally, we propose that the developed method can be extrapolated to other crops for routine analysis and can be used to determine the pre-harvest intervals (PHIs), thus preventing the development of antibiotic resistance genes in humans and in the environment.
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Md Musfiqur
authors.initials: MM
authors.lastname: Rahman
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea; Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, 12211 Giza, Egypt. Electronic address: abdelaty44@hotmail.com.
authors.forename: A M
authors.initials: AM
authors.lastname: Abd El-Aty
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
authors.forename: Sung-Woo
authors.initials: SW
authors.lastname: Kim
authors.affiliation: Jeonnam Bioindustry Foundation, Bio Control Research Center Immyeon, Gokseonggun, Jeollanamdo, Republic of Korea.
authors.forename: Tae-Woong
authors.initials: TW
authors.lastname: Na
authors.affiliation: Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul 143-701, Republic of Korea.
authors.forename: Ho-Chul
authors.initials: HC
authors.lastname: Shin
authors.affiliation: Department of Agro-food Safety, National Academy of Agricultural Science, Rural Development Administration, Wanju 560-500, Republic of Korea.
authors.forename: Su-Myeong
authors.initials: SM
authors.lastname: Hong
authors.affiliation: Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea. Electronic address: jhshim@chonnam.ac.kr.
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Shim
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D036901:Petasites / Q000737:chemistry*; D011741:Pyrimidine Nucleosides / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D011741:Pyrimidine Nucleosides; C002458:polyoxorim
keywords: Butterbur; Dissipation; Fungicide; Half-life; Polyoxin D; UPLC-MS/MS