title: Single Fluorescent Probe for Dual-Imaging Viscosity and H2O2in Mitochondria with Different Fluorescence Signals in Living Cells.
abstract: Mitochondria, as essential and interesting organelles within the eukaryotic cells, play key roles in a variety of pathologies, and its abnormalities are closely associated with Alzheimer's disease (AD) and other diseases. Studies have shown that the abnormal of viscosity and concentration of hydrogen peroxide in mitochondria were all associated with AD. Accordingly, the detection of viscosity and hydrogen peroxide in mitochondria has attracted great attention. However, it remains a great challenge to explore a single probe, which can dual-detect the viscosity and H2O2in mitochondria. Herein, in two ways to prevent the twisted internal charge transfer (TICT) process, we designed and sythesized the first dual-detection fluorescent probe Mito-VH that can visualize viscosity and H2O2in mitochondria with different fluorescence signals in living cells.
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan , Jinan, Shandong 250022, P. R. China.
authors.forename: Mingguang
authors.identifier: 0000-0003-2673-8348
authors.initials: M
authors.lastname: Ren
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan , Jinan, Shandong 250022, P. R. China.
authors.forename: Beibei
authors.initials: B
authors.lastname: Deng
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan , Jinan, Shandong 250022, P. R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhou
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan , Jinan, Shandong 250022, P. R. China.
authors.forename: Xiuqi
authors.initials: X
authors.lastname: Kong
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan , Jinan, Shandong 250022, P. R. China.
authors.forename: Jian-Yong
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan , Jinan, Shandong 250022, P. R. China.
authors.forename: Weiying
authors.initials: W
authors.lastname: Lin
mesh_terms: D000818:Animals; D002470:Cell Survival; D004579:Electron Transport; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism*; D051379:Mice; D008856:Microscopy, Fluorescence / Q000379:methods*; D008928:Mitochondria / Q000378:metabolism*; D008958:Models, Molecular; D008968:Molecular Conformation; D000067996:RAW 264.7 Cells; D014783:Viscosity
chemical_list: D005456:Fluorescent Dyes; D006861:Hydrogen Peroxide