title: Hydrophilic polymeric nanoparticles prepared from Delonix galactomannan with low cytotoxicity for ocular drug delivery.
abstract: Delonix is a galactomannan polysaccharide extracted from the endosperm of Delonix regia plant. This study aims at the development of Delonix nanoparticle and assesses its potential for ocular delivery by evaluating its in-vitro stability, toxicity and cellular uptake. Fluorescent nanoparticles (BODIPY-loaded nanoparticles) were prepared by a Quality-by-Design modified nanoprecipitation technique. Optimized nanoparticles had mean sizes <240nm, PdI<0.2 and zeta potential of <-30mV. Mixture of surfactants with different hydrophilic-lipophilic balance controlled nanoparticle swelling. Nanoparticles, which were stable in the presence of simulated lachrymal fluid and lysozyme also sustained the release of BODIPY. In-vitro studies suggest no toxicity of the nanoparticles in concentration range of 100-1483.3μg/mL on retinal and corneal epithelial cells. Flow cytometry and confocal microscopy techniques showed that retinal cells but not corneal cells, uptake 18% of the nanoparticles. Therefore, Delonix nanoparticles could be a safe and promising tool for ocular drug delivery.
authors.affiliation: University of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto, SP, Brazil; Department of Pharmaceutics, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria.
authors.forename: Abayomi T
authors.initials: AT
authors.lastname: Ogunjimi
authors.affiliation: University of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
authors.forename: Shaiani M G
authors.initials: SMG
authors.lastname: Melo
authors.affiliation: University of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
authors.forename: Carem G
authors.initials: CG
authors.lastname: Vargas-Rechia
authors.affiliation: University of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto, SP, Brazil.
authors.forename: Flávio S
authors.initials: FS
authors.lastname: Emery
authors.affiliation: University of São Paulo, School of Pharmaceutical Sciences of Ribeirão Preto, Ribeirão Preto, SP, Brazil. Electronic address: rvianna@fcfrp.usp.br.
authors.forename: Renata F V
authors.initials: RFV
authors.lastname: Lopez
mesh_terms: D060433:Administration, Ophthalmic; D002460:Cell Line; D003315:Cornea / Q000166:cytology; D004337:Drug Carriers / Q000737:chemistry*; D016503:Drug Delivery Systems; D004847:Epithelial Cells / Q000187:drug effects*; D007887:Fabaceae / Q000737:chemistry*; D005690:Galactose / Q000031:analogs & derivatives; D006801:Humans; D008351:Mannans / Q000737:chemistry*; D053758:Nanoparticles; D010316:Particle Size; D011108:Polymers; D012160:Retina / Q000166:cytology
chemical_list: D004337:Drug Carriers; D008351:Mannans; D011108:Polymers; C012990:galactomannan; D005690:Galactose
keywords: Cellular uptake; Cytotoxicity; Delonix polymeric nanoparticles; Ocular delivery; Quality by design