title: Laserpitium zernyi Hayek Flower and Herb Extracts: Phenolic Compounds, and Anti-edematous, Antioxidant, and Antimicrobial Activities.
abstract: Phenolic compounds and different biological activities of the dry MeOH extracts of the flowers and the herb (aerial parts without flowers) of Laserpitium zernyi Hayek (Apiaceae) were investigated. The total phenolic contents in the extracts were determined spectrophotometrically using Folin-Ciocalteu reagent. In both extracts, apigenin, luteolin, their 7-O-glucosides, and chlorogenic acid were detected by HPLC. Identified phenolics were quantified in both extracts, except luteolin in L. zernyi herb extract. The extracts (p.o.) were tested for anti-edematous activity in a model of carrageenan (i.pl.) induced rat paw edema. Antioxidant activity of the extracts was assessed by FRAP assay and DPPH and•OH radicals scavenging tests. Antimicrobial activity was investigated using broth microdilution test against five Gram-positive and three Gram-negative bacteria, as well as against two strains of Candida albicans. The polyphenol-richer flower extract exerted higher anti-edematous and antioxidant activities. The herb extract exhibited better antimicrobial effect against Micrococcus luteus, Enterococcus faecalis, Bacillus subtilis, and Pseudomonas aeruginosa, while against other tested microorganisms, the activity of both extracts was identical. Demonstrated biological activities of L. zernyi flower and herb extracts represent a good basis for their further investigation as potential new herbal medicinal raw materials.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.|Receptor Research Laboratories, Nuclear Receptor Lab, VIB Medical Biotechnology Center, VIB, Ghent University, Albert Baertsoenkaai 3, BE-9000, Ghent.
authors.forename: Višnja B
authors.initials: VB
authors.lastname: Popović
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Maja A
authors.initials: MA
authors.lastname: Tomić
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Radica M
authors.initials: RM
authors.lastname: Stepanović-Petrović
authors.affiliation: Department of Pharmacology, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Ana M
authors.initials: AM
authors.lastname: Micov
authors.affiliation: Department of Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Marina T
authors.initials: MT
authors.lastname: Milenković
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Silvana D
authors.initials: SD
authors.lastname: Petrović
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, V. Stepe 450, RS-11221, Belgrade.
authors.forename: Ljuboš J
authors.initials: LJ
authors.lastname: Ušjak
authors.affiliation: Natural History Museum, Njegoševa 51, RS-11000, Belgrade.
authors.forename: Marjan S
authors.initials: MS
authors.lastname: Niketić
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000302:isolation & purification / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology; D019661:Apiaceae / Q000737:chemistry*; D002176:Candida albicans / Q000187:drug effects; D004487:Edema / Q000188:drug therapy; D035264:Flowers / Q000737:chemistry; D006090:Gram-Negative Bacteria / Q000187:drug effects; D006094:Gram-Positive Bacteria / Q000187:drug effects; D010636:Phenols / Q000302:isolation & purification; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D051381:Rats
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts
keywords: Anti-edematous activities; Antimicrobial activities; Antioxidant activities; Laserpitium zernyi aerial parts; Polyphenols