title: Effect of calcium-binding peptide from Pacific cod (Gadus macrocephalus) bone on calcium bioavailability in rats.
abstract: Bone collagen peptide with high affinity to Ca was extracted from Pacific cod (Gadus macrocephalus) bone. FTIR spectra of calcium-binding bone collagen peptide showed that band at 3381cm-1shifted to 3361cm-1, 1455cm-1moved to 1411cm-1, and amide II became deeper valley, compared with that of bone collagen peptide. This peptide was sequenced by Q-TOF-MS and sequences of Gly-Pro-Glu-Gly, Gly-Glu-Lys, Gly-Pro-Leu-Gly and Gly-Leu-Pro-Gly appeared repeatedly in some peptides. From SEM, after chelated with calcium, the loose and porous structure turned into granular structure. From the animal experiment, Ca apparent absorption rate, Ca retention rate and femur Ca content of calcium-binding bone collagen peptide group were significantly higher than those of model and CaCO3groups (P<0.05), while serum ALP was significantly lower than model group (P<0.05) and similar to control group. The results suggested that calcium-binding bone collagen peptide could improve bioavailability of Ca and thus prevented Ca deficiency.
authors.affiliation: College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Peng
authors.affiliation: College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China. Electronic address: houhu@ouc.edu.cn.
authors.forename: Hu
authors.initials: H
authors.lastname: Hou
authors.affiliation: College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Food Science and Engineering, Ocean University of China, No. 5, Yu Shan Road, Qingdao, Shandong Province 266003, PR China. Electronic address: bfli@ouc.edu.cn.
authors.forename: Bafang
authors.initials: B
authors.lastname: Li
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001682:Biological Availability; D002118:Calcium / Q000378:metabolism*; D002136:Calcium, Dietary; D002135:Calcium-Binding Proteins / Q000494:pharmacology*; D003094:Collagen / Q000378:metabolism; D005269:Femur / Q000187:drug effects* / Q000378:metabolism; D048388:Gadiformes / Q000378:metabolism*; D008297:Male; D010455:Peptides / Q000737:chemistry; D011485:Protein Binding; D051381:Rats
chemical_list: D002136:Calcium, Dietary; D002135:Calcium-Binding Proteins; D010455:Peptides; D003094:Collagen; D002118:Calcium
keywords: Calcium absorption; Calcium bioavailability; Calcium-binding peptide; FTIR; Pacific cod bone