title: Study of mouse induced pluripotent stem cell transplantation intoWistar albino rat cochleae after hair cell damage.
abstract: OBJECTIVE
  As the regeneration capacity of hair cells is limited, inner ear stem cell therapies hold promise. Effects of mouse induced pluripotent stem cells (IPSCs) on Wistar albino rats (WARs) with hearing impairment were investigated.
  
  
  METHODS
  Thirty-five adult WARs with normal hearing were divided into 4 groups. Excluding the study group (n = 15), the other three groups served as control groups for ototoxicity and IPSC injection models. IPSC injections were performed via cochleostomy after a retroauricular approach. Auditory functions were evaluated with auditory brainstem responses (ABRs) before and after the injections. After a final hearing assessment the WARs were sacrificed and cochleae were extracted to see the biologic behavior of IPSCs in the inner ear by light microscopy and immunohistochemistry.
  
  
  RESULTS
  There were no significant differences in the click-ABR thresholds in the study group after IPSC transplantation. The mean hearing threshold in the study group after ototoxic agent injection was 53.2 dB (10-90 dB). There was no significant difference between groups (P > 0.05) and no differentiated stem cells were observed immunohistochemically.
  
  
  CONCLUSIONS
  Transplanted IPSCs did not show a therapeutic effect in this trial. We discuss potential pitfalls and factors affecting the therapeutic effect.
authors.affiliation: Department of Otorhinolaryngology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Mustafa Kürşat
authors.initials: MK
authors.lastname: Gökcan
authors.affiliation: Department of Otorhinolaryngology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Selçuk
authors.initials: S
authors.lastname: Mülazimoğlu
authors.affiliation: Republic of Turkey Ministry of Health, Keçiören Education and Research Hospital, Ankara, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Ocak
authors.affiliation: Department of Surgery, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
authors.forename: Pınar
authors.initials: P
authors.lastname: Can
authors.affiliation: Department of Surgery, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Çalışkan
authors.affiliation: Department of Surgery, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey.
authors.forename: Ömer
authors.initials: Ö
authors.lastname: Beşaltı
authors.affiliation: Department of Pathology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Serpil
authors.initials: S
authors.lastname: Dizbay Sak
authors.affiliation: Department of Pathology, Faculty of Medicine, Ankara University, Ankara, Turkey.
authors.forename: Gülşah
authors.initials: G
authors.lastname: Kaygusuz
mesh_terms: D000818:Animals; D003051:Cochlea; D016057:Evoked Potentials, Auditory, Brain Stem; D006197:Hair; D057026:Induced Pluripotent Stem Cells; D051379:Mice; D051381:Rats; D017208:Rats, Wistar
keywords: Hearing loss; induced pluripotent stem cell; therapy; transplantation