title: [Imaging Blood Flow and Pulsation of Retinal Vessels with Full-Field Swept-Source OCT].
abstract: Optical coherence tomography (OCT) uses interference to image the retina with high axial resolution. In the last 25 years, new technologies have permitted a steady increase in imaging speed, which made it possible to enlarge the imaged field and to avoid motion artefacts. The speed and precision of retinal imaging is now limited by photodamage of the retina caused by the focused OCT beam and by the speed of the scanning mechanics. Full-field swept-source (FF-SS)-OCT decreases irradiance on the retina and dispenses moving parts by using a camera to acquire the full volume of the retina in parallel. Here we show that FF-SS-OCT is rapid and precise enough to image pulsation in the retina induced by the heart beat. Series of OCT volumes 1.8 × 0.7 mm wide and 1.8 mm deep were recorded in young volunteers over a few cycles of the heart beat. Morphology of the retinal vessels, blood flow and tissue motion as caused by vessel pulsation were calculated from the OCT data. FF-SS-OCT was able to visualise the main structures of the neuronal retina, including vessels and small capillaries and without any motion artefacts. Information on three different dynamic processes was obtained from only one recorded series of OCT volumes: pulsation of blood flow and blood pressure in retinal vessels as well as pulsation of the choroid. Delays between arterial and venous pulse and delay between pulsation in retinal and choroidal vessels were calculated. With a time resolution of 0.5 ms, FF-SS-OCT is able to visualise previously unmeasurably fast changes in the retina, including the propagation of pulse waves.
authors.affiliation: Institut für Biomedizinische Optik, Universität zu Lübeck, Lübeck.
authors.forename: H
authors.initials: H
authors.lastname: Spahr
authors.affiliation: Thorlabs GmbH, Lübeck.
authors.forename: D
authors.initials: D
authors.lastname: Hillmann
authors.affiliation: Institut für Biomedizinische Optik, Universität zu Lübeck, Lübeck.
authors.forename: C
authors.initials: C
authors.lastname: Hain
authors.affiliation: Institut für Biomedizinische Optik, Universität zu Lübeck, Lübeck.
authors.forename: C
authors.initials: C
authors.lastname: Pfäffle
authors.affiliation: Medizinisches Laserzentrum Lübeck GmbH, Lübeck.
authors.forename: H
authors.initials: H
authors.lastname: Sudkamp
authors.affiliation: Institut für Biomedizinische Optik, Universität zu Lübeck, Lübeck.
authors.forename: G
authors.initials: G
authors.lastname: Franke
authors.affiliation: Medizinisches Laserzentrum Lübeck GmbH, Lübeck.
authors.forename: P
authors.initials: P
authors.lastname: Koch
authors.affiliation: Institut für Biomedizinische Optik, Universität zu Lübeck, Lübeck.
authors.forename: G
authors.initials: G
authors.lastname: Hüttmann
mesh_terms: D000792:Angiography / Q000379:methods; D001783:Blood Flow Velocity / Q000502:physiology*; D001794:Blood Pressure / Q000502:physiology*; D003941:Diagnostic Techniques, Ophthalmological; D005260:Female; D006801:Humans; D007090:Image Interpretation, Computer-Assisted / Q000379:methods*; D008297:Male; D011673:Pulsatile Flow / Q000502:physiology*; D015203:Reproducibility of Results; D012171:Retinal Vessels / Q000502:physiology*; D012680:Sensitivity and Specificity; D041623:Tomography, Optical Coherence / Q000379:methods*; D055815:Young Adult