title: Brevundimonas canariensis sp. nov., isolated from roots of Triticum aestivum.
abstract: A bacterial strain designated GTAE24T was isolated from a root of wheat growing in soil from the Canary Islands, Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Brevundimonas with Brevundimonas abyssalisTAR-001T as its closest relative at 99.4 % similarity. DNA-DNA hybridization studies showed an average of 38 % relatedness between strain GTAE24T and the type strain of B. abyssalis. Cells were Gram-stain-negative and motile by polar flagella. The strain was positive for oxidase and weakly positive for catalase. Gelatin, starch and casein were not hydrolysed. Growth was supported by many carbohydrates and organic acids as carbon source. Ubiquinone Q-10 was the predominant isoprenoid quinone and C18 : 1ω7c/C18 : 1ω6c (summed feature 8) and C16 : 0 were the major fatty acids. The major polar lipids were phosphatidylglycerol, 1,2-di-O-acyl-3-O-[d-glucopyranosyl-(1,4)-α-d-glucopyranuronosyl] glycerol, 1,2-diacyl-3-O-[6'-phosphatidyl-α-d-glucopyranosyl] glycerol, 1,2-di-O-acyl-3-O-α-d-glucopyranosyl glycerol, and 1,2-di-O-acyl-3-O-α-d-glucopyranuronosyl glycerol. The DNA G+C content was 63.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain GTAE24T should be considered as representing a novel species of the genus Brevundimonas, for which the name Brevundimonas canariensis sp. nov. is proposed. The type strain is GTAE24T (=LMG 29500T=CECT 9126T).
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.
authors.forename: Esther
authors.initials: E
authors.lastname: Menéndez
authors.affiliation: Departamento de Bioquímica, Microbiología, Biología Celular y Genética, Universidad de La Laguna, Tenerife, Spain.
authors.forename: Juan
authors.initials: J
authors.lastname: Pérez-Yepes
authors.affiliation: School of Biology, Newcastle University, Newcastle-upon-Tyne, UK.
authors.forename: Lorena
authors.initials: L
authors.lastname: Carro
authors.affiliation: Instituto de Ciencias Agrarias, Consejo Superior de Investigaciones Científicas (ICA-CSIC), Madrid, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: Fernández-Pascual
authors.affiliation: Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.|Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
authors.forename: Martha-Helena
authors.initials: MH
authors.lastname: Ramírez-Bahena
authors.affiliation: School of Biology, Newcastle University, Newcastle-upon-Tyne, UK.
authors.forename: Hans-Peter
authors.initials: HP
authors.lastname: Klenk
authors.affiliation: Departamento de Bioquímica, Microbiología, Biología Celular y Genética, Universidad de La Laguna, Tenerife, Spain.
authors.forename: Milagros
authors.initials: M
authors.lastname: León Barrios
authors.affiliation: Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.|Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), Salamanca, Spain.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Peix
authors.affiliation: Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.|Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA-CSIC, Salamanca, Spain.
authors.forename: Encarna
authors.initials: E
authors.lastname: Velázquez
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D042063:Caulobacteraceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013030:Spain; D014908:Triticum / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C025204:Ubiquinone Q2