title: Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Applications in Quantitative Proteomics.
abstract: Recent advances in inductively coupled plasma mass spectrometry (ICP-MS) hyphenated to different separation techniques have promoted it as a valuable tool in protein/peptide quantification. These emerging ICP-MS applications allow absolute quantification by measuring specific elemental responses. One approach quantifies elements already present in the structure of the target peptide (e.g. phosphorus and sulphur) as natural tags. Quantification of these natural tags allows the elucidation of the degree of protein phosphorylation in addition to absolute protein quantification. A separate approach is based on utilising bi-functional labelling substances (those containing ICP-MS detectable elements), that form a covalent chemical bond with the protein thus creating analogs which are detectable by ICP-MS. Based on the previously established stoichiometries of the labelling reagents, quantification can be achieved. This technique is very useful for the design of precise multiplexed quantitation schemes to address the challenges of biomarker screening and discovery. This review discusses the capabilities and different strategies to implement ICP-MS in the field of quantitative proteomics.
authors.affiliation: Almac House, 20 Seagoe Industrial Estate, Craigavon, BT63 5QD, United Kingdom.
authors.forename: Osama
authors.initials: O
authors.lastname: Chahrour
authors.forename: John
authors.initials: J
authors.lastname: Malone
mesh_terms: D000595:Amino Acid Sequence; D002614:Chelating Agents / Q000737:chemistry; D006801:Humans; D007553:Isotope Labeling / Q000379:methods; D010455:Peptides / Q000032:analysis* / Q000737:chemistry; D010758:Phosphorus / Q000737:chemistry; D010766:Phosphorylation; D011506:Proteins / Q000032:analysis* / Q000737:chemistry; D040901:Proteomics / Q000295:instrumentation / Q000379:methods*; D012643:Selenium / Q000737:chemistry; D013054:Spectrophotometry, Atomic / Q000295:instrumentation / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013455:Sulfur / Q000737:chemistry
chemical_list: D002614:Chelating Agents; D010455:Peptides; D011506:Proteins; D010758:Phosphorus; D013455:Sulfur; D012643:Selenium
keywords: ICP-MS; Proteomics; biomarkers; lanthanides; phosphorylation; speciation; species-specific isotope dilution