title: Biophysical and biological characterization of intraoral multilayer membranes as potential carriers: A new drug delivery system for dentistry.
abstract: The current study developed through layer-by-layer deposition a multilayer membrane for intraoral drug delivery and analyzed the biochemical, functional, and biological properties of this membrane. For that purpose, we designed a three-layer chlorhexidine-incorporated membrane composed by pure chitosan and alginate. The biochemical, functional, and biological properties were analyzed by the following tests: degradation in saliva medium; controlled drug release; water absorption, mass loss; pH analysis; and biocompatibility through fibroblast cell viability by MTT assay. All tests were conducted at three different periods (24, 48 and 72hours). The results demonstrated that hybrid membranes composed by alginate and chitosan with glycerol had greater water absorption and mass loss in buffer solution and in artificial saliva. The controlled drug release test revealed that the hybrid membrane exhibited greater drug release (0.075%). All chlorhexidine-incorporated membranes reduced the cell viability, and chitosan membranes with and without glycerol did not interfere with fibroblast viability. The biochemical and biophysical characteristics of the designed membranes and the findings of cell viability tests indicate great potential for application in Dentistry.
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Community Dentistry, Discipline of Pediatric Dentistry, Bauru School of Dentistry, University of São Paulo, Brazil.
authors.forename: Mariana Dos Santos
authors.initials: MDS
authors.lastname: Silva
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Community Dentistry, Discipline of Pediatric Dentistry, Bauru School of Dentistry, University of São Paulo, Brazil.
authors.forename: Natalino Lourenço
authors.initials: NL
authors.lastname: Neto
authors.affiliation: Course on Textiles and Fashion, School of Arts, Sciences and Humanities, University of São Paulo, Brazil.
authors.forename: Silgia Aparecida
authors.initials: SA
authors.lastname: da Costa
authors.affiliation: Course on Textiles and Fashion, School of Arts, Sciences and Humanities, University of São Paulo, Brazil.
authors.forename: Sirlene Maria
authors.initials: SM
authors.lastname: da Costa
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Community Dentistry, Discipline of Pediatric Dentistry, Bauru School of Dentistry, University of São Paulo, Brazil.
authors.forename: Thais Marchini
authors.initials: TM
authors.lastname: Oliveira
authors.affiliation: Department of Biological Sciences, Discipline of Biochemistry, Bauru School of Dentistry, University of São Paulo, Brazil. Electronic address: rodrigocardoso@usp.br.
authors.forename: Rodrigo Cardoso de
authors.initials: RC
authors.lastname: Oliveira
authors.affiliation: Department of Pediatric Dentistry, Orthodontics and Community Dentistry, Discipline of Pediatric Dentistry, Bauru School of Dentistry, University of São Paulo, Brazil.
authors.forename: Maria Aparecida Andrade Moreira
authors.initials: MAAM
authors.lastname: Machado
mesh_terms: D000464:Alginates / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D000818:Animals; D048271:Chitosan / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002710:Chlorhexidine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D003813:Dentistry; D004337:Drug Carriers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D020723:Glucuronic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006603:Hexuronic Acids / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D008567:Membranes, Artificial; D051379:Mice; D041681:NIH 3T3 Cells
chemical_list: D000464:Alginates; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006603:Hexuronic Acids; D008567:Membranes, Artificial; D020723:Glucuronic Acid; D048271:Chitosan; D002710:Chlorhexidine
keywords: Alginate; Cell viability; Chitosan; Chlorhexidine; Controlled drug release; Layer-by-layer coatings