title: Microwave-induced biomimetic approach for hydroxyapatite coatings of chitosan scaffolds.
abstract: Simulated body fluid (SBF) can form calcium phosphates on osteoinductive materials, so it is widely used for coating of bone scaffolds to mimic natural extracellular matrix (ECM). However, difficulties of bulk coating in 3D scaffolds and the necessity of long process times are the common problems for coating with SBF. In the present study, a microwave-assisted process was developed for rapid and internal coating of chitosan scaffolds. The scaffolds were fabricated as superporous hydrogel (SPH) by combining microwave irradiation and gas foaming methods. Then, they were immersed into 10x  SBF-like solution and homogenous bone-like hydroxyapatite (HA) coating was achieved by microwave treatment at 600W without the need of any nucleating agent. Cell culture studies with MC3T3-E1 preosteoblasts showed that microwave-assisted biomimetic HA coating process could be evaluated as an efficient and rapid method to obtain composite scaffolds for bone tissue engineering.
authors.affiliation: Hacettepe University, Bioengineering Department, Ankara, Turkey.
authors.forename: Gökçe
authors.initials: G
authors.lastname: Kaynak Bayrak
authors.affiliation: Hacettepe University, Bioengineering Department, Ankara, Turkey.
authors.forename: T Tolga
authors.initials: TT
authors.lastname: Demirtaş
authors.affiliation: Hacettepe University, Bioengineering Department, Ankara, Turkey; Hacettepe University, Chemical Engineering Department, Ankara, Turkey. Electronic address: menemse@hacettepe.edu.tr.
authors.forename: Menemşe
authors.initials: M
authors.lastname: Gümüşderelioğlu
mesh_terms: D040761:Biomimetic Materials; D032701:Biomimetics; D018786:Bone Substitutes / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D008872:Microwaves; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D018786:Bone Substitutes; D048271:Chitosan; D017886:Durapatite
keywords: Bone-tissue engineering; Chitosan; Hydroxyapatite; Microwave; Simulated body fluid