title: Optimization of Spectral and Spatial Conditions to Improve Super-Resolution Imaging of Plasmonic Nanoparticles.
abstract: Interactions between fluorophores and plasmonic nanoparticles modify the fluorescence intensity, shape, and position of the observed emission pattern, thus inhibiting efforts to optically super-resolve plasmonic nanoparticles. Herein, we investigate the accuracy of localizing dye fluorescence as a function of the spectral and spatial separations between fluorophores (Alexa 647) and gold nanorods (NRs). The distance at which Alexa 647 interacts with NRs is varied by layer-by-layer polyelectrolyte deposition while the spectral separation is tuned by using NRs with varying localized surface plasmon resonance (LSPR) maxima. For resonantly coupled Alexa 647 and NRs, emission to the far field through the NR plasmon is highly prominent, resulting in underestimation of NR sizes. However, we demonstrate that it is possible to improve the accuracy of the emission localization when both the spectral and spatial separations between Alexa 647 and the LSPR are optimized.
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: A Swarnapali
authors.initials: AS
authors.lastname: De Silva Indrasekara
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Shuang
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Franziska
authors.initials: F
authors.lastname: Hollenhorst
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Benjamin S
authors.initials: BS
authors.lastname: Hoener
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Anneli
authors.initials: A
authors.lastname: Hoggard
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Sishan
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Materials Science and Nanoengineering, Rice University , 6100 Main Street, MS-325, Houston, Texas 77005, United States.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Villarreal
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Yi-Yu
authors.initials: YY
authors.lastname: Cai
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Lydia
authors.initials: L
authors.lastname: Kisley
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: Derry
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.
authors.forename: Wei-Shun
authors.initials: WS
authors.lastname: Chang
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.|Department of Materials Science and Nanoengineering, Rice University , 6100 Main Street, MS-325, Houston, Texas 77005, United States.
authors.forename: Eugene R
authors.initials: ER
authors.lastname: Zubarev
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.|Department of Materials Science and Nanoengineering, Rice University , 6100 Main Street, MS-325, Houston, Texas 77005, United States.
authors.forename: Emilie
authors.initials: E
authors.lastname: Ringe
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.|Department of Electrical and Computer Engineering, Rice University , 6100 Main Street, MS-366, Houston, Texas 77005, United States.
authors.forename: Stephan
authors.initials: S
authors.lastname: Link
authors.affiliation: Department of Chemistry, Rice University , 6100 Main Street, MS-60, Houston, Texas 77005, United States.|Department of Electrical and Computer Engineering, Rice University , 6100 Main Street, MS-366, Houston, Texas 77005, United States.
authors.forename: Christy F
authors.identifier: http://orcid.org/0000-0003-4163-6497
authors.initials: CF
authors.lastname: Landes