title: Development and validation of a simple and fast method for simultaneous determination of aflatoxin B1and sterigmatocystin in grains.
abstract: In this study, a simple, fast, sensitive and reliable method was developed for the simultaneous determination of aflatoxin B1(AFB1) and sterigmatocystin (STC) in rice, wheat, maize, oat, rye and barley grains based on a modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). AFB1and STC were extracted using 95% acetonitrile in water and cleaned up using N-Propylethane-1,2-diamine (PSA). The method was validated according to the SANCO/12571/2013 regulation of the European Union (EU). Recoveries at three spiked levels of 0.5, 2.5 and 5.0μg/kg ranged from 77.7% to 119.7% and the relative standard deviations (RSDs) were between 1.3% and 12.8%. Limits of detection (LOD) were 0.03 and 0.02μg/kg for AFB1and STC, respectively. The limit of quantification (LOQ) was 0.5μg/kg for both AFB1and STC. The proposed method was successfully applied to determine AFB1and STC levels in ten non-processed rice samples from Guangdong province in China.
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; Public Monitoring Center for Agro-product of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Key Laboratory of Testing and Evaluation for Agro-product Safety and Quality, Ministry of Agriculture, Guangzhou 510640, China; Laboratory of Quality and Safety Risk Assessment for Agro-product (Guangzhou), Ministry of Agriculture, Guangzhou 510640, China. Electronic address: zyr520zyr@163.com.
authors.forename: Yarong
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Public Monitoring Center for Agro-product of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Key Laboratory of Testing and Evaluation for Agro-product Safety and Quality, Ministry of Agriculture, Guangzhou 510640, China; Laboratory of Quality and Safety Risk Assessment for Agro-product (Guangzhou), Ministry of Agriculture, Guangzhou 510640, China. Electronic address: 598427183@qq.com.
authors.forename: Jianxiang
authors.initials: J
authors.lastname: Huang
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: lyma1203@cau.edu.cn.
authors.forename: Liyan
authors.initials: L
authors.lastname: Ma
authors.affiliation: Public Monitoring Center for Agro-product of Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China; Key Laboratory of Testing and Evaluation for Agro-product Safety and Quality, Ministry of Agriculture, Guangzhou 510640, China; Laboratory of Quality and Safety Risk Assessment for Agro-product (Guangzhou), Ministry of Agriculture, Guangzhou 510640, China. Electronic address: wfhwqs@163.com.
authors.forename: Fuhua
authors.initials: F
authors.lastname: Wang
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis*; D002853:Chromatography, Liquid / Q000379:methods*; D002523:Edible Grain / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D057230:Limit of Detection; D012275:Oryza / Q000737:chemistry; D013241:Sterigmatocystin / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D013241:Sterigmatocystin; D016604:Aflatoxin B1
keywords: Aflatoxin B(1); Grains; LC-MS/MS; QuEChERS; Sterigmatocystin