title: The protective effects of hydrogen on HO-1 expression in the brainafter focal cerebral ischemia reperfusion in rats.
abstract: OBJECTIVE
  The aim of this study was to investigate whether a hydrogen administration can produce neuroprotective effects after brain ischemia reperfusion in rats.
  
  
  METHODS
  A brain ischemia reperfusion injury was induced by a 2-h left middle cerebral artery occlusion (MCAO) using an intraluminal filament, followed by 46 h of reperfusion. A hydrogen-rich saline (1 mL/kg body weight i.p.) was administered at the beginning of reperfusion. Saline (1 mL/kg)-treated animals were used as the control. Sham-operated animals were also used. Subsequently, 48 h after the MCAO, histological alternations, heme oxygenase-1 (HO-1) expression, and levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in the cerebral cortex and the hippocampus were examined.
  
  
  RESULTS
  Hydrogen significantly alleviated brain tissue histological damage, promoted HO-1 expression, upregulated levels of SOD, and decreased the levels of MDA in brain tissue after the ischemia reperfusion injury.
  
  
  CONCLUSIONS
  The results suggest that the neuroprotective effects of hydrogen may be mediated by promoting HO-1 expression and attenuated the oxidative injury.
authors.affiliation: Department of Critical Care Medicine, Yu Huang Ding Hospital, Qingdao University, Yan Tai, P.R. China.
authors.forename: Xifeng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Histology and Embryology, Bin Zhou Medical College, Yan Tai, P.R. China.
authors.forename: Lianshuang
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, Bin Zhou Medical College, Yan Tai, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Department of Histology and Embryology, Bin Zhou Medical College, Yan Tai, P.R. China.
authors.forename: Tongshen
authors.initials: T
authors.lastname: Liu
mesh_terms: D000818:Animals; D002545:Brain Ischemia; D051547:Heme Oxygenase-1; D006859:Hydrogen; D008315:Malondialdehyde; D018696:Neuroprotective Agents; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury; D013482:Superoxide Dismutase
chemical_list: D018696:Neuroprotective Agents; D008315:Malondialdehyde; D006859:Hydrogen; D051547:Heme Oxygenase-1; D013482:Superoxide Dismutase
keywords: Hydrogen; brain; cerebral ischemia reperfusion; heme oxygenase-1; rats