title: Mannose-binding lectin 2 (Mbl2) gene polymorphisms are related to protein plasma levels, but not to heart disease and infection by Chlamydia.
abstract: The presence of the single nucleotide polymorphisms in exon 1 of the mannose-binding lectin 2 (MBL2) gene was evaluated in a sample of 159 patients undergoing coronary artery bypass surgery (71 patients undergoing valve replacement surgery and 300 control subjects) to investigate a possible association between polymorphisms and heart disease with Chlamydia infection. The identification of the alleles B and D was performed using real time polymerase chain reaction (PCR) and of the allele C was accomplished through PCR assays followed by digestion with the restriction enzyme. The comparative analysis of allelic and genotypic frequencies between the three groups did not reveal any significant difference, even when related to previous Chlamydia infection. Variations in the MBL plasma levels were influenced by the presence of polymorphisms, being significantly higher in the group of cardiac patients, but without representing a risk for the disease. The results showed that despite MBL2 gene polymorphisms being associated with the protein plasma levels, the polymorphisms were not enough to predict the development of heart disease, regardless of infection with both species of Chlamydia.
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: M A F
authors.initials: MA
authors.lastname: Queiroz
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: S T M
authors.initials: ST
authors.lastname: Gomes
authors.affiliation: Fundação de Hematologia e Hemoterapia do Pará, Belém, PA, Brasil.
authors.forename: N C C
authors.initials: NC
authors.lastname: Almeida
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: M I M
authors.initials: MI
authors.lastname: Souza
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: S R C F
authors.initials: SR
authors.lastname: Costa
authors.affiliation: Fundação de Hematologia e Hemoterapia do Pará, Belém, PA, Brasil.
authors.forename: R B
authors.initials: RB
authors.lastname: Hermes
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: S S
authors.initials: SS
authors.lastname: Lima
authors.affiliation: Hospital de Clínicas Gaspar Vianna, Belém, PA, Brasil.
authors.forename: M M
authors.initials: MM
authors.lastname: Zaninotto
authors.affiliation: Hospital Beneficência Portuguesa, Belém, PA, Brasil.
authors.forename: M A A
authors.initials: MA
authors.lastname: Fossa
authors.affiliation: Hospital Beneficência Portuguesa, Belém, PA, Brasil.
authors.forename: M A
authors.initials: MA
authors.lastname: Maneschy
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: R N
authors.initials: RN
authors.lastname: Martins-Feitosa
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: V N
authors.initials: VN
authors.lastname: Azevedo
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: L F A
authors.initials: LF
authors.lastname: Machado
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: M O G
authors.initials: MO
authors.lastname: Ishak
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: R
authors.initials: R
authors.lastname: Ishak
authors.affiliation: Laboratório de Virologia, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brasil.
authors.forename: A C R
authors.initials: AC
authors.lastname: Vallinoto
mesh_terms: D016022:Case-Control Studies; D002690:Chlamydia Infections / Q000097:blood* / Q000175:diagnosis / Q000235:genetics*; D003430:Cross-Sectional Studies; D005260:Female; D005787:Gene Frequency; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006349:Heart Valve Diseases / Q000097:blood / Q000382:microbiology* / Q000601:surgery; D006801:Humans; D008297:Male; D037601:Mannose-Binding Lectin / Q000097:blood* / Q000235:genetics*; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide
chemical_list: C445619:MBL2 protein, human; D037601:Mannose-Binding Lectin