title: Tumorigenicity and Validity of Fluorescence Labelled Mesenchymal and Epithelial Human Oral Cancer Cell Lines in Nude Mice.
abstract: Tumorigenicity and metastatic activity can be visually monitored in cancer cells that were labelled with stable fluorescence. The aim was to establish and validate local and distant spread of subcutaneously previously injected fluorescence transduced human tongue cancer cell lines of epithelial and mesenchymal phenotype in nude mice. A total of 32 four-week-old male athymic Balb/c nude mice were randomly allocated into 4 groups (n= 8). A single dose of 0.3 mL PBS containing 1 × 107 of four different cancer cell-lines (UM1, UM1-GFP, UM2, and UM2-RFP) was injected subcutaneously into the right side of their posterolateral back. Validity assessment of the labelled cancer cells' tumorigenicity was assessed by physical examination, imaging, and histology four weeks after the injection. The tumor take rate of cancer cells was similar in animals injected with either parental or transduced cancer cells. Transduced cancer cells in mice were easily detectable in vivo and after cryosection using fluorescent imaging. UM1 cells showed increased tumor take rate and mean tumor volume, presenting with disorganized histopathological patterns. Fluorescence labelled epithelial and mesenchymal human tongue cancer cell lines do not change in tumorigenicity or cell phenotype after injection in vivo.
authors.affiliation: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Wei Xin
authors.identifier: 0000-0002-9859-7460
authors.initials: WX
authors.lastname: Cai
authors.affiliation: Discipline of Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Li Wu
authors.identifier: 0000-0002-7593-0208
authors.initials: LW
authors.lastname: Zheng
authors.affiliation: Discipline of Oral Rehabilitation, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Li
authors.identifier: 0000-0003-4305-3017
authors.initials: L
authors.lastname: Ma
authors.affiliation: Department of Oral and Maxillofacial Surgery, Guanghua School of Stomatology, Hospital of Stomatology, Guangdong Provincial Key Laboratory of Stomatology, Sun Yat-sen University, Guangzhou, China.
authors.forename: Hong Zhang
authors.initials: HZ
authors.lastname: Huang
authors.affiliation: Discipline of Oral Diagnosis and Polyclinics, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Ru Qing
authors.initials: RQ
authors.lastname: Yu
authors.affiliation: Discipline of Oral and Maxillofacial Surgery, Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Roger A
authors.identifier: 0000-0003-4666-7567
authors.initials: RA
authors.lastname: Zwahlen
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D016616:Cryoultramicrotomy; D004848:Epithelium / Q000473:pathology*; D005456:Fluorescent Dyes / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism; D006801:Humans; D008297:Male; D008648:Mesoderm / Q000473:pathology*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D009062:Mouth Neoplasms / Q000473:pathology*; D015203:Reproducibility of Results; D040521:Subcutaneous Tissue / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D005456:Fluorescent Dyes; D049452:Green Fluorescent Proteins