title: Optimized reverse primer for 16S-RFLP analysis and genomovar assignment of Flavobacterium columnare.
authors.affiliation: United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Aquatic Animal Health Research Unit, Auburn, AL, USA.
authors.forename: B R
authors.initials: BR
authors.lastname: LaFrentz
authors.affiliation: United States Department of Agriculture-Agricultural Research Service (USDA-ARS), Aquatic Animal Health Research Unit, Auburn, AL, USA.
authors.forename: J C
authors.initials: JC
authors.lastname: García
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.|Department Microbiology, Faculty of Science, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
authors.forename: H T
authors.initials: HT
authors.lastname: Dong
authors.affiliation: USDA-ARS, Warmwater Aquaculture Research Unit, Thad Cochran National Warmwater Aquaculture Center, Stoneville, MS, USA.
authors.forename: G C
authors.initials: GC
authors.lastname: Waldbieser
authors.affiliation: Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.
authors.forename: C
authors.initials: C
authors.lastname: Rodkhum
authors.affiliation: MSD Animal Health, MSD Animal Health Innovation Pte Ltd, Singapore city, Singapore.
authors.forename: F S
authors.initials: FS
authors.lastname: Wong
authors.affiliation: MSD Animal Health, MSD Animal Health Innovation Pte Ltd, Singapore city, Singapore.
authors.forename: S F
authors.initials: SF
authors.lastname: Chang
mesh_terms: D000818:Animals; D015373:Bacterial Typing Techniques / Q000379:methods*; D004587:Electrophoresis, Agar Gel / Q000379:methods; D005393:Fish Diseases / Q000145:classification / Q000382:microbiology*; D045826:Flavobacteriaceae Infections / Q000145:classification / Q000382:microbiology / Q000662:veterinary*; D005417:Flavobacterium / Q000145:classification* / Q000302:isolation & purification; D016680:Genome, Bacterial; D016133:Polymerase Chain Reaction / Q000379:methods; D012150:Polymorphism, Restriction Fragment Length; D012329:RNA, Bacterial / Q000032:analysis*; D012336:RNA, Ribosomal, 16S / Q000032:analysis*
chemical_list: D012329:RNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 
  Flavobacterium columnare
  ; 16S rRNA; columnaris disease; genomovar; restriction fragment length polymorphism