title: The graphene oxide and chitosan biopolymer loads TiO2for antibacterial and preservative research.
abstract: Microorganism breeding is a known cause of food spoilage and disease transmission. Aspergillus niger (A. niger) and Bacillus subtilis (B. subtilis) are examples of microorganisms that cause deterioration of fresh fruits and vegetables during storage, which can be a serious threat to human health. In this work, we synthesized a self-assembled film of graphene oxide (GO) and chitosan (CS) biopolymers with titanium dioxide (TiO2) nanoparticles embedded in its surface. We then characterized its antibacterial and preservative properties. We found that these non-cytotoxic nanometer-scale films, especially when the ratio of graphene oxide, chitosan and titanium dioxide nanoparticles in the nanocomposites is 1:20:4, exhibited high antibacterial activity against the biofilm-forming strains A. niger and B. subtilis. The preservation capacity of the nanocomposites was evaluated by enzymatic experiments. The nanocomposites did not show any cytotoxicity against mammalian somatic cells and plant cells. Altogether, this work demonstrated that the nanocomposites disrupted microbial film formation while avoiding internalization by animal and plant cells. Due to their selectivity and safety, these nanocomposites demonstrate potential as antimicrobial coatings for food preservation.
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Weirui
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Xie
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Xiaoquan
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Na
authors.initials: N
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Jinan University, Guangzhou 510632, China. Electronic address: tliuliu@jnu.edu.cn.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001412:Bacillus subtilis / Q000187:drug effects / Q000378:metabolism*; D048271:Chitosan / Q000737:chemistry*; D005519:Food Preservation / Q000379:methods*; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D011310:Preservatives, Pharmaceutical / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D011310:Preservatives, Pharmaceutical; C009495:titanium dioxide; D006108:Graphite; D048271:Chitosan; D014025:Titanium
keywords: Antibacterial; Biocompatibility; Graphene oxide; Preservation; Titanium dioxide