title: Effect of interleukin-1β and lipoxin A4in human endometriotic stromal cells: Proteomic analysis.
abstract: OBJECTIVE
  Lipoxin A4(LXA4) can function as an endogenous 'breaking signal' in inflammation and plays an important role in the progression of endometriosis. The proteome responses to interleukin-1β (IL-1β) or LXA4in human endometriotic stromal cells (ESC) are not well understood.
  
  
  METHODS
  In this study, primary ESC were cultured from ovarian endometriosis tissue. Three groups were established: the control group; the IL-1β stimulation group; and the IL-1β and LXA4incubation group. Proteins were assessed on 2-D polyacrylamide gel electrophoresis (2D-PAGE), and differentially expressed protein spots were further identified on matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MALDI-TOF-MS). Wound healing and transwell assays were performed to assess the migration and invasion of ESC after treatment.
  
  
  RESULTS
  In total, 40 differentially expressed protein spots were identified successfully on MALDI-TOF-MS. The proteins identified were related to cell structure, metabolism, signal transduction, protein synthesis and membrane structure, processes that may be involved in the development of endometriosis. Vinculin and IL-4 were further analyzed on western blot and quantitative real-time polymerase chain reaction. Moreover, LXA4could suppress the migration and invasion of ESC induced by IL-1β.
  
  
  CONCLUSIONS
  LXA4may inhibit the progression of endometriosis partly by lowering or raising the effect of IL-1β, mediated via some inflammation-related proteins (e.g. vinculin) and immune response-related protein (e.g. IL-4) in vitro.
authors.affiliation: State Key Laboratory of Cellular Stress Biology and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Rong-Feng
authors.initials: RF
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Cellular Stress Biology and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Hui-Ming
authors.initials: HM
authors.lastname: Yang
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Zhou
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Li-Rong
authors.initials: LR
authors.lastname: Zhang
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Jian-Bing
authors.initials: JB
authors.lastname: Bai
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Dian-Chao
authors.initials: DC
authors.lastname: Lin
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Tai-Wei
authors.initials: TW
authors.lastname: Ng
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Song-Juan
authors.initials: SJ
authors.lastname: Dai
authors.affiliation: Department of Obstetrics and Gynecology, First Affiliated Hospital of Xiamen University, Xiamen, China.
authors.forename: Qiong-Hua
authors.initials: QH
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Cellular Stress Biology and Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, China.
authors.forename: Qing-Xi
authors.initials: QX
authors.lastname: Chen
mesh_terms: D000328:Adult; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D004715:Endometriosis / Q000188:drug therapy / Q000378:metabolism*; D004717:Endometrium / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005260:Female; D006801:Humans; D053583:Interleukin-1beta / Q000187:drug effects / Q000378:metabolism*; D044045:Lipoxins / Q000494:pharmacology*; D040901:Proteomics / Q000379:methods*; D017154:Stromal Cells / Q000187:drug effects / Q000378:metabolism*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; C508585:IL1B protein, human; D053583:Interleukin-1beta; D044045:Lipoxins; C040527:lipoxin A4
keywords: 2-D polyacrylamide gel electrophoresis; endometriosis; interleukin-1β; lipoxin A4; proteomics