title: Construction and amperometric biosensing performance of a novel platform containing carbon nanotubes-zinc phthalocyanine and a conducting polymer.
abstract: A novel glucose oxidase (GOx) based amperometric biosensor utilizing a conducting polymer (CP), multi walled carbon nanotubes (MWCNTs) and a novel water soluble zinc phthalocyanine (ZnPc) was constructed. For this purpose, a novel ZnPc was synthesized to examine the role of being a part of support material for enzyme deposition. High water solubility was achieved with the introduction of tetra quaternized imidazolyl moieties at the peripheral positions of phthalocyanine. In order to fabricate the proposed biosensor, a graphite electrode was firstly modified with poly[9,9-di-(2-ethylhexyl)- fluorenyl-2,7-diyl] end capped with N,N-Bis(4- methylphenyl)-4-aniline (PFLA) and MWCNTs. Then, GOx was co-immobilized with ZnPc onto the modified surface. To the best our knowledge, a sensor design which combines conjugated polymer/MWCNTs/ZnPc was attempted for the first time and this approach resulted in improved biosensor characteristics. The constructed biosensor showed a linear response for glucose between 0.025-1.0mM with a detection limit of 0.018mM. KMappand sensitivity values were calculated as 0.53mM and 82.18μAmm-1cm-2, respectively. Moreover, scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques were used to investigate the surface modifications. Finally, fabricated biosensor was tested on beverages for glucose detection successfully.
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey.
authors.forename: Ece
authors.initials: E
authors.lastname: Buber
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Turkey.
authors.forename: Abdulcelil
authors.initials: A
authors.lastname: Yuzer
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey.
authors.forename: Saniye
authors.initials: S
authors.lastname: Soylemez
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey.
authors.forename: Melis
authors.initials: M
authors.lastname: Kesik
authors.affiliation: Advanced Technology Research & Application Center, Mersin University, Turkey; Department of Energy Systems Engineering, Faculty of Technology, Mersin University, Turkey.
authors.forename: Mine
authors.initials: M
authors.lastname: Ince
authors.affiliation: Department of Chemistry, Middle East Technical University, Ankara 06800, Turkey; Department of Biotechnology, Middle East Technical University, Ankara 06800, Turkey; Department of Polymer Science and Technology, Middle East Technical University, Ankara 06800, Turkey; The Center for Solar Energy Research and Application (GUNAM), Middle East Technical University, Ankara 06800, Turkey. Electronic address: toppare@metu.edu.tr.
authors.forename: Levent
authors.initials: L
authors.lastname: Toppare
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology; D015374:Biosensing Techniques / Q000379:methods*; D004553:Electric Conductivity; D004566:Electrodes; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D005947:Glucose / Q000032:analysis / Q000737:chemistry; D005949:Glucose Oxidase / Q000737:chemistry / Q000378:metabolism*; D007211:Indoles / Q000737:chemistry*; D054833:Isoindoles; D037742:Nanotubes, Carbon / Q000737:chemistry*; D009942:Organometallic Compounds / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D012995:Solubility; D014867:Water / Q000737:chemistry; D017967:Zinc Compounds
chemical_list: D004800:Enzymes, Immobilized; D007211:Indoles; D054833:Isoindoles; D037742:Nanotubes, Carbon; D009942:Organometallic Compounds; D011108:Polymers; D017967:Zinc Compounds; D014867:Water; C052159:Zn(II)-phthalocyanine; D005949:Glucose Oxidase; D005947:Glucose
keywords: Amperometric biosensor; Carbon nanotube; Conducting polymer; Glucose biosensor; Zinc phthalocyanine