title: Characterization of apple stem grooving virus and apple chlorotic leaf spot virus identified in a crab apple tree.
abstract: Apple stem grooving virus (ASGV), apple chlorotic leaf spot virus (ACLSV), and prunus necrotic ringspot virus (PNRSV) were identified in a crab apple tree by small RNA deep sequencing. The complete genome sequence of ACLSV isolate BJ (ACLSV-BJ) was 7554 nucleotides and shared 67.0%-83.0% nucleotide sequence identity with other ACLSV isolates. A phylogenetic tree based on the complete genome sequence of all available ACLSV isolates showed that ACLSV-BJ clustered with the isolates SY01 from hawthorn, MO5 from apple, and JB, KMS and YH from pear. The complete nucleotide sequence of ASGV-BJ was 6509 nucleotides (nt) long and shared 78.2%-80.7% nucleotide sequence identity with other isolates. ASGV-BJ and the isolate ASGV_kfp clustered together in the phylogenetic tree as an independent clade. Recombination analysis showed that isolate ASGV-BJ was a naturally occurring recombinant.
authors.affiliation: College of Plant Science and Technology, Beijing University of Agriculture, Beijing, 102206, China. lyq@bua.edu.cn.
authors.forename: Yongqiang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China.
authors.forename: Congliang
authors.initials: C
authors.lastname: Deng
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Bian
authors.affiliation: Plant Laboratory of Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 101312, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Chinese Society of Inspection and Quarantine, Beijing, 100029, China. zhouq@bjciq.gov.cn.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D052677:Flexiviridae / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D016679:Genome, Viral; D027845:Malus / Q000821:virology*; D008969:Molecular Sequence Data; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D014764:Viral Proteins / Q000235:genetics
chemical_list: D014764:Viral Proteins