title: Vitamin D contributes to mast cell stabilization.
abstract: OBJECTIVE
  Mast cells are the major effector cells in allergic disorders and many other informatory disorders. The mechanism of mast cell stabilization is not fully understood. Cumulative reports indicate that vitamin D (VitD) contributes to the homeostasis in the body. This study tests a hypothesis that VitD is required in the maintenance of the stability of mast cells.
  
  
  METHODS
  The stability of mast cell lines, HMC1 cells, RBL-2H3 cells, p815 cells, and mouse bone marrow-derived mast cells (BMMC) was tested in the presence or absence of VitD3.
  
  
  RESULTS
  Mast cells activated automatically in a VitD-deficient environment. Exposure to calcitriol in the culture increased the expression of VitD receptor (VDR) in mast cells. VDR formed complexes with Lyn in mast cells to inhibit the binding of Lyn to the β chain of FcεRI and MyD88, which decreased the phosphorylation of Syk, decreased the levels of MAPK and NF-κB. VDR bound to the promoter of TNF-α to decrease the acetylation of histone H3/H4, RNA polymerase II and OCT1 (a transcription factor of TNF-α) at the promoter locus and repressed the expression of TNF-α in mast cells.
  
  
  CONCLUSIONS
  The data demonstrate that VitD is required to maintain the stability of mast cells. The deficiency of VitD results in mast cell activation.
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors.forename: Z-Q
authors.identifier: http://orcid.org/0000-0002-7353-8827
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: X-X
authors.initials: XX
authors.lastname: Li
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors.forename: S-Q
authors.initials: SQ
authors.lastname: Qiu
authors.affiliation: The Fifth Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: M-G
authors.initials: MG
authors.lastname: Li
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.|Brain Body Institute, McMaster University, Hamilton, ON, Canada.
authors.forename: L-T
authors.initials: LT
authors.lastname: Yang
authors.affiliation: Brain Body Institute, McMaster University, Hamilton, ON, Canada.
authors.forename: L-J
authors.initials: LJ
authors.lastname: Li
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.|Longgang ENT Hospital, Shenzhen, China.
authors.forename: S
authors.initials: S
authors.lastname: Wang
authors.affiliation: The Fifth Hospital, Zhengzhou University, Zhengzhou, China.
authors.forename: P-Y
authors.initials: PY
authors.lastname: Zheng
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: Z-G
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine, Shenzhen, China.
authors.forename: P-C
authors.initials: PC
authors.lastname: Yang
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D042002:Chromatin Assembly and Disassembly / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005512:Food Hypersensitivity / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D007073:Immunoglobulin E / Q000276:immunology; D018836:Inflammation Mediators / Q000378:metabolism; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000502:physiology*; D051379:Mice; D018345:Mice, Knockout; D011401:Promoter Regions, Genetic; D011485:Protein Binding; D018167:Receptors, Calcitriol / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism; D014807:Vitamin D / Q000378:metabolism* / Q000494:pharmacology; D019061:src-Family Kinases / Q000378:metabolism
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D018167:Receptors, Calcitriol; D014409:Tumor Necrosis Factor-alpha; D014807:Vitamin D; D007073:Immunoglobulin E; C060902:lyn protein-tyrosine kinase; D019061:src-Family Kinases
keywords: Lyn tyrosine kinase; Syk tyrosine kinase; calcitriol; mast cell; vitamin D