title: Effects of ZnSO4-induced peripheral anosmia on zebrafish behavior and physiology.
abstract: Olfaction plays a key role in modulating behavioral and physiological responses of various animal species, including fishes. Olfactory deficits can be induced in fish experimentally, and utilized to examine the role of olfaction in their normal and pathological behaviors. Here, we examine whether experimental anosmia, evoked by ZnSO4in adult zebrafish can be associated with behavioral and/or physiological responses. We show that experimental ZnSO4-induced anosmia caused acute, but not prolonged, anxiogenic-like effects on zebrafish behavior tested in the novel tank test. The procedure also elevated whole-body cortisol levels in zebrafish. Moreover, ZnSO4 treatment, but not sham, produced damage to olfactory epithelium, inducing overt basal cell vacuolization and intercellular edema. The loss of olfaction, assessed by the fish food preference behavior in the aquatic Y-maze, was present 1h, but not 24h, after the treatment. Collectively, this suggests that transient experimental anosmia by ZnSO4modulates zebrafish behavior and olfaction, which can be used to evoke and assess their stress-related anxiety-like states.
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS, 97105-900, Brazil.
authors.forename: Murilo S
authors.initials: MS
authors.lastname: Abreu
authors.affiliation: Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS, 99052-900, Brazil.
authors.forename: Ana C V V
authors.initials: AC
authors.lastname: Giacomini
authors.affiliation: Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS, 99052-900, Brazil.
authors.forename: Rubens
authors.initials: R
authors.lastname: Rodriguez
authors.affiliation: Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, 199034, Russia; Ural Federal University, Yekaterinburg, 62002, Russia.
authors.forename: Allan V
authors.initials: AV
authors.lastname: Kalueff
authors.affiliation: Programa de Pós-Graduação em Farmacologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima, 1000, Cidade Universitária, Camobi, Santa Maria, RS, 97105-900, Brazil; Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS, 99052-900, Brazil; Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo (UPF), BR 285, São José, Passo Fundo, RS, 99052-900, Brazil. Electronic address: lbarcellos@upf.br.
authors.forename: Leonardo J G
authors.initials: LJ
authors.lastname: Barcellos
mesh_terms: D000704:Analysis of Variance; D000818:Animals; D001252:Astringents / Q000633:toxicity*; D015331:Cohort Studies; D004195:Disease Models, Animal; D005260:Female; D006854:Hydrocortisone / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000187:drug effects*; D000857:Olfaction Disorders / Q000139:chemically induced* / Q000473:pathology; D018709:Statistics, Nonparametric; D013997:Time Factors; D015027:Zebrafish; D019287:Zinc Sulfate / Q000633:toxicity*
chemical_list: D001252:Astringents; D019287:Zinc Sulfate; D006854:Hydrocortisone
keywords: Anxiety; Emotional behavior; Experimental anosmia protocol; Olfactory deprivation; Zebrafish