title: Ecotropic viral integration site I regulates alpha1, 6-fucosyl transferase expression and blocks erythropoiesis in chronic myeloid leukemia.
abstract: Although BCR-ABL is the hallmark genetic abnormality of chronic myeloid leukemia (CML), secondary molecular events responsible for the evolution of the disease to blast crisis are yet to be deciphered. Taking into account the significant association of ecotropic viral integration site I (EVI1) in CML drug resistance, it is necessary to decipher the other roles played by EVI1 in CML disease progression. In this regard, we cross-hybridized three microarray datasets and deduced a set of 11 genes that seems to be regulated by EVI1 in CML. We observed a strong correlation between EVI1 and alpha1, 6-fucosyltransferase (FUT8) in the chronic phase of the disease and both of them were found to be up-regulated with the progression of the disease. Knockdown of EVI1 in a CML cell line not only down-regulated FUT8, but also rendered the cells towards erythroid differentiation. Our study shows the involvement of EVI1 and FUT8 axis in blocking erythropoiesis in CML.
authors.affiliation: a Institute of Life Sciences , Bhubaneswar , India.
authors.forename: Nivedita
authors.initials: N
authors.lastname: Kuila
authors.affiliation: a Institute of Life Sciences , Bhubaneswar , India.
authors.forename: Kasturi Bala
authors.initials: KB
authors.lastname: Nayak
authors.affiliation: a Institute of Life Sciences , Bhubaneswar , India.
authors.forename: Arundhati
authors.initials: A
authors.lastname: Halder
authors.affiliation: a Institute of Life Sciences , Bhubaneswar , India.
authors.forename: Subramaniam
authors.initials: S
authors.lastname: Agatheeswaran
authors.affiliation: b Sparsh Hospital and Critical Care , Bhubaneswar , India.
authors.forename: Ghanashyam
authors.initials: G
authors.lastname: Biswas
authors.affiliation: b Sparsh Hospital and Critical Care , Bhubaneswar , India.
authors.forename: Sutapa
authors.initials: S
authors.lastname: Biswas
authors.affiliation: c Lab Care and Diagnostics , Cuttack , India.
authors.forename: Naresh Chandra
authors.initials: NC
authors.lastname: Pattnayak
authors.affiliation: a Institute of Life Sciences , Bhubaneswar , India.
authors.forename: Soumen
authors.initials: S
authors.lastname: Chakraborty
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D004920:Erythropoiesis / Q000235:genetics*; D005647:Fucosyltransferases / Q000235:genetics*; D015973:Gene Expression Regulation, Leukemic; D055785:Gene Knockdown Techniques; D056726:Genetic Association Studies; D006801:Humans; D020014:K562 Cells; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000097:blood / Q000235:genetics* / Q000378:metabolism* / Q000473:pathology; D000074008:MDS1 and EVI1 Complex Locus Protein / Q000378:metabolism*; D051379:Mice; D009367:Neoplasm Staging
chemical_list: D000074008:MDS1 and EVI1 Complex Locus Protein; C063575:MECOM protein, human; D005647:Fucosyltransferases; C020559:Glycoprotein 6-alpha-L-fucosyltransferase
keywords: CML; EVI1; FUT8; erythropoiesis