title: In vitrogastrointestinal digestion study of a novel bio-tofu with special emphasis on the impact of microbial transglutaminase.
abstract: We have developed a novel bio-tofu, made from mixed soy and cow milk (MSCM), usingLactobacillus helveticusMB2-1 andLactobacillus plantarumB1-6 incorporated with microbial transglutaminase (MTGase) as coagulant. MTGase was added to improve the textural properties and suit for cooking. However, the effect of MTGase on the digestion of mixed-protein fermented by lactic acid bacteria was unclear. This study aimed at evaluating the effect of MTGase on protein digestion of bio-tofu under simulated gastrointestinal digestion condition. The results showed that addition of MTGase could affect the particle size distribution, degree of hydrolysis, the content of soluble proteins and free amino acids. Based on the electrophoresis data, MTGase addition enhanced protein polymerization. During gastric and intestinal digestion process, proteins from bio-tofu were degraded into low molecular mass peptides. Our results suggested that incorporation of MTGase could lead to enzymatic modification of proteins of bio-tofu which may help in controlling energy intake and decrease the chance of food allergy.
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University , Nanjing , P. R. China.
authors.forename: Guangliang
authors.initials: G
authors.lastname: Xing
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University , Nanjing , P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Rui
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University, Nanjing, P. R. China; Huai'an Academy of Nanjing Agricultural University, Huai'an, P. R. China.
authors.forename: Mei
authors.initials: M
authors.lastname: Jiang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University , Nanjing , P. R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University , Nanjing , P. R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Guan
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University , Nanjing , P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: College of Food Science and Technology, Nanjing Agricultural University , Nanjing , P. R. China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Dong
keywords: Bio-tofu; In vitro gastrointestinal digestion; Lactic acid bacteria; Microbial transglutaminase (MTGase)