title: Silencing of Btbd7 Inhibited Epithelial-Mesenchymal Transition and Chemoresistance in CD133+Lung Carcinoma A549 Cells.
abstract: Cancer stem cells (CSCs) are responsible for tumorigenesis and recurrence, so targeting CSCs is an effective method to potentially cure cancer. BTB/POZ domain-containing protein 7 (Btbd7) has been found in various cancers, including lung cancer and liver cancer, but the role of Btbd7 in non-small cell lung cancer (NSCLC), CSC self-renewal, and chemoresistance is still unknown. Therefore, in this study we found that the ratio of tumor sphere formation and stem cell transcription factors in CD133+ cells was dramatically enhanced compared to parental cells, which indicated successful sorting of CD133+ cells from A549. Meanwhile, Btbd7 and the markers of the epithelial-mesenchymal transition (EMT) process were more highly expressed in CD133+ cells than in parental cells. Silencing of Btbd7 significantly inhibited the self-renewal and EMT process in CD133+ cells. Furthermore, we found that downregulation of Btbd7 promoted cell apoptosis and increased the sensitivity to paclitaxel in CD133+ and parental cells. In conclusion, our results suggest that Btbd7 is a promising agent for the inhibition of survival and chemoresistance of cancer stem-like cells of NSCLC, which may act as an important therapeutic target in NSCLC.
authors.forename: Li-Zhou
authors.initials: LZ
authors.lastname: Fang
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Zhang
authors.forename: Ling
authors.initials: L
authors.lastname: Liu
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Fu
authors.forename: Jing-Kui
authors.initials: JK
authors.lastname: Shu
authors.forename: Jia-Gang
authors.initials: JG
authors.lastname: Feng
authors.forename: Xiao
authors.initials: X
authors.lastname: Liang
mesh_terms: D000072283:A549 Cells; D000071916:AC133 Antigen / Q000378:metabolism*; D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents / Q000494:pharmacology; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement; D049109:Cell Proliferation; D019008:Drug Resistance, Neoplasm / Q000235:genetics*; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015870:Gene Expression; D020868:Gene Silencing; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009363:Neoplasm Proteins / Q000235:genetics*; D014411:Neoplastic Stem Cells / Q000187:drug effects / Q000378:metabolism
chemical_list: D000071916:AC133 Antigen; D048868:Adaptor Proteins, Signal Transducing; D000970:Antineoplastic Agents; C436517:BTBD7 protein, human; D009363:Neoplasm Proteins