title: Cell Surface Nano-modulation for Non-invasive in vivo Near-IR Stem Cell Monitoring.
abstract: A stem cell tracking system is in high demand for the determination of cell destinations and for the validation of cell therapeutic efficacy in regenerative transplantation. To date, near-infrared (NIR) imaging technology has received considerable attention in cell behavior monitoring, owing to its patient compatibility, easy accessibility and cost effectiveness. Conventionally, in vivo cell tracking has been visualized by direct in-cell staining with NIR, where it may be achieved by complicated genetic engineering. Such genetic amendment techniques have suffered from serious challenges, which can destroy a cell's metabolism and can accidentally incur unexpected carcinoma. Herein we demonstrate a novel cell nano-modulation method for noninvasive stem cell monitoring. It is simply achieved by conjugating stem cells with lipid-supported, NIR-tagged, polymeric nanoparticles. These engineered cells, which are designated as NIR-labeled light-emitting stem cells (LESCs), maintain their biochemical functionality (i.e., differentiation, quantum efficacy, etc.) even after conjugation. LESCs were used for in situ stem cell monitoring at inoculation sites. It is speculated that the LESC technique could provide a new preparative methodology for in vivo cell tracking in advanced diagnostic medicine, where cell behavior is a critical issue.
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Woo Jung
authors.initials: WJ
authors.lastname: Shin
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Ji Soo
authors.initials: JS
authors.lastname: Yuk
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Lunjakorn
authors.initials: L
authors.lastname: Amornkitbamrung
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Min Su
authors.initials: MS
authors.lastname: Jang
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: In Hyun
authors.initials: IH
authors.lastname: Song
authors.affiliation: Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 85 dong, Gwangk-ro 1, Gwanak-gu, Seoul, 151-747, South Korea.
authors.forename: Soon Won
authors.initials: SW
authors.lastname: Choi
authors.affiliation: Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 85 dong, Gwangk-ro 1, Gwanak-gu, Seoul, 151-747, South Korea.
authors.forename: Insung
authors.initials: I
authors.lastname: Kang
authors.affiliation: Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 85 dong, Gwangk-ro 1, Gwanak-gu, Seoul, 151-747, South Korea.
authors.forename: Jin Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: College of Animal Bioscience and Technology, Department of Bioindustrial Technologies, Konkuk University, 120, Neungdong-ro, Gwangjin-gu, Seoul, 05029, South Korea.
authors.forename: Hojae
authors.initials: H
authors.lastname: Bae
authors.affiliation: Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul National University, 85 dong, Gwangk-ro 1, Gwanak-gu, Seoul, 151-747, South Korea.
authors.forename: Kyung-Sun
authors.initials: KS
authors.lastname: Kang
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.|SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, 2066, Seobu-ro, Jangan-gu, Suwon, Gyeonggi-do, 440-746, South Korea.
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
mesh_terms: D058948:Cell Tracking; D006801:Humans; D007259:Infrared Rays; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D013234:Stem Cells / Q000166:cytology*
chemical_list: D011108:Polymers
keywords: in vivo monitoring; nanotechnology; near-IR; stem cells; surface modulation