title: Detection of pantothenic acid-immunoreactive neurons in the rat lateral septal nucleus by a newly developed antibody.
abstract: BACKGROUND
  The available immunohistochemical techniques have documented restricted distribution of vitamins in the mammalian brain. The aim of the study was to develop a highly specific antiserum directed against pantothenic acid to explore the presence of this vitamin in the mammalian brain.
  
  
  METHODS
  According to ELISA tests, the anti-pantothenic acid antiserum used showed a good affinity (10-8 M) and specificity. The antiserum was raised in rabbits. Using an indirect immunoperoxidase technique, the mapping of pantothenic acid-immunoreactive structures was carried out in the rat brain.
  
  
  RESULTS
  Pantothenic acid-immunoreactive perikarya were exclusively found in the intermediate part of the lateral septal nucleus. These cells were generally small, round, fusiform or pyramidal and showed 2-3 long (50-100 μm) immunoreactive dendrites. Any immunoreactive axons containing pantothenic acid were detected.
  
  
  CONCLUSIONS
  The very restricted anatomical distribution of the pantothenic acid suggests that this vitamin could be involved in some specific neurophysiological mechanisms.
authors.affiliation: Gemacbio, Saint Jean d'Illac, France Institut pour le Développement de la Recherche en Pathologie Humaine et Thérapeutique (IDRPHT), Talence, France Institute of Neurosciences of Castilla y León (INCYL), Laboratory of Neuroanatomy of the Peptidergic Systems, University of Salamanca, Salamanca, Spain. mangasam@usal.es.
authors.forename: Arturo
authors.initials: A
authors.lastname: Mangas
authors.forename: Javier
authors.initials: J
authors.lastname: Yajeya
authors.forename: Noelia
authors.initials: N
authors.lastname: Gonzalez
authors.forename: Marianne
authors.initials: M
authors.lastname: Husson
authors.forename: Michel
authors.initials: M
authors.lastname: Geffard
authors.forename: Rafael
authors.initials: R
authors.lastname: Coveñas
mesh_terms: D000818:Animals; D000906:Antibodies / Q000097:blood / Q000276:immunology*; D000917:Antibody Formation; D000918:Antibody Specificity; D001369:Axons / Q000276:immunology; D001921:Brain / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay; D007106:Immune Sera / Q000276:immunology; D007124:Immunoenzyme Techniques; D007150:Immunohistochemistry; D008297:Male; D009474:Neurons / Q000166:cytology / Q000276:immunology*; D010205:Pantothenic Acid / Q000276:immunology*; D011817:Rabbits; D051381:Rats; D017208:Rats, Wistar; D012686:Septal Nuclei / Q000166:cytology / Q000276:immunology*
chemical_list: D000906:Antibodies; D007106:Immune Sera; D010205:Pantothenic Acid
keywords: IHC; brain; lateral septal nucleus; new antibody; pantothenic acid; rat; vitamin B5