title: Environment friendly approach for size controllable synthesis of biocompatible Silver nanoparticles using diastase.
abstract: A green, facile method for the size selective synthesis of silver nanoparticles (AgNPs) using diastase as green reducing and stabilizing agent is reported. The thiol groups present in the diastase are mainly responsible for the rapid reaction rate of silver nanoparticles synthesis. The variation in the size and morphology of AgNPs were studied by changing the pH of diastase. The prepared silver nanoparticles were characterized by using UV-vis, XRD, FTIR, TEM and SAED. The FTIR analysis revealed the stabilization of diastase molecules on the surface of AgNPs. Additionally, in-vitro cytotoxicity experiments concluded that the cytotoxicity of the as-synthesized AgNPs towards mouse fibroblast (3T3) cell lines is dose and size dependent. Furthermore, the present method is an alternative to the traditional chemical methods of size controlled AgNPs synthesis.
authors.affiliation: The Key laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, National Engineering Lab for Textile Fiber Materials and Processing Technology (Zhejiang), College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China; Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Sireesh Babu
authors.initials: SB
authors.lastname: Maddinedi
authors.affiliation: Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India. Electronic address: badalkmandal@hotmail.com.
authors.forename: Badal Kumar
authors.initials: BK
authors.lastname: Mandal
authors.affiliation: Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Kiran Kumar
authors.initials: KK
authors.lastname: Anna
mesh_terms: D016475:3T3 Cells; D000681:Amylases / Q000737:chemistry*; D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity / Q000648:ultrastructure; D051379:Mice; D046529:Microscopy, Electron, Transmission; D010316:Particle Size; D012834:Silver / Q000737:chemistry* / Q000633:toxicity
chemical_list: D012834:Silver; D000681:Amylases
keywords: 3T3 cell lines; Cytotoxicity; Diastase; Mouse fibroblast; Silver NPs