title: Critical Role of Different Immobilized Biocatalysts of a Given Lipase in the Selective Ethanolysis of Sardine Oil.
abstract: Different immobilized derivatives of two lipases were tested as catalysts of the synthesis of ethyl esters of omega-3 fatty acids during the ethanolysis of sardine oil in solvent-free systems at 25 °C. Lipases from Thermomyces lanuginosus (TLL) and Lecitase Ultra (a phospholipase with lipolytic activity) were studied. Lipases were adsorbed on hydrophobic Sepabeads C18 through the open active center and on an anion-exchanger Duolite with the active center exposed to the reaction medium. TLL-Sepabeads derivatives exhibit a high activity of 9 UI/mg of immobilized enzyme, and they are 20-fold more active than TLL-Duolite derivatives and almost 1000-fold more active than Lipozyme TL IM (the commercial derivative from Novozymes). Lecitase-Sepabeads exhibit a high selectivity for the synthesis of the ethyl ester of EPA that is 43-fold faster than the synthesis of the ethyl ester of DHA.
authors.affiliation: Instituto de Catálisis, CSIC , Campus UAM-CSIC, 28049 Madrid, Spain.
authors.forename: Sonia
authors.initials: S
authors.lastname: Moreno-Perez
authors.affiliation: Department of Biochemistry and Microbiology, Univ Estadual Paulista at Rio Claro - 8 UNESP , Rio Claro, SP 13506-900, Brazil.
authors.forename: Daniela Flavia Machado
authors.initials: DF
authors.lastname: Turati
authors.affiliation: Departamento de Quı́mica e Tecnologia, Instituto de Química - UNESP , 14800-069, 10 Araraquara, SP, Brazil.
authors.forename: Janaina Pires
authors.initials: JP
authors.lastname: Borges
authors.affiliation: Departamento de Quı́mica Fı́sica Aplicada, Universidad Autónoma , 28049 Madrid, Sapin.
authors.forename: Pilar
authors.initials: P
authors.lastname: Luna
authors.affiliation: Departamento de Quı́mica Fı́sica Aplicada, Universidad Autónoma , 28049 Madrid, Sapin.
authors.forename: Francisco Javier
authors.identifier: http://orcid.org/0000-0002-0775-6957
authors.initials: FJ
authors.lastname: Señorans
authors.affiliation: Instituto de Catálisis, CSIC , Campus UAM-CSIC, 28049 Madrid, Spain.
authors.forename: Jose M
authors.identifier: http://orcid.org/0000-0003-1627-6522
authors.initials: JM
authors.lastname: Guisan
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL) CSIC-UAM , 28049 Madrid, Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: Fernandez-Lorente
mesh_terms: D001203:Ascomycota / Q000201:enzymology*; D055162:Biocatalysis; D004800:Enzymes, Immobilized / Q000737:chemistry; D004951:Esterification; D015525:Fatty Acids, Omega-3 / Q000737:chemistry*; D005395:Fish Oils / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry*; D008049:Lipase / Q000737:chemistry*
chemical_list: D004800:Enzymes, Immobilized; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D005656:Fungal Proteins; D008049:Lipase
keywords: lipase immobilization; lipases in solvent-free systems; lipases stability; selectivity EPA vs DHA