title: Label-Free Neuroproteomics of the Hippocampal-Accumbal Circuit Reveals Deficits in Neurotransmitter and Neuropeptide Signaling in Mice Lacking Ethanol-Sensitive Adenosine Transporter.
abstract: The neural circuit of the dorsal hippocampus (dHip) and nucleus accumbens (NAc) contributes to cue-induced learning and addictive behaviors, as demonstrated by the escalation of ethanol-seeking behaviors observed following deletion of the adenosine equilibrative nucleoside transporter 1 (ENT1-/-) in mice. Here we perform quantitative LC-MS/MS neuroproteomics in the dHip and NAc of ENT1-/-mice. Using Ingenuity Pathway Analysis, we identified proteins associated with increased long-term potentiation, ARP2/3-mediated actin cytoskeleton signaling and protein expression patterns suggesting deficits in glutamate degradation, GABAergic signaling, as well as significant changes in bioenergetics and energy homeostasis (oxidative phosphorylation, TCA cycle, and glycolysis). These pathways are consistent with previously reported behavioral and biochemical phenotypes that typify mice lacking ENT1. Moreover, we validated decreased expression of the SNARE complex protein VAMP1 (synaptobrevin-1) in the dHip as well as decreased expression of pro-dynorphin (PDYN), neuroendocrine convertase (PCSK1), and Leu-Enkephalin (dynorphin-A) in the NAc. Taken together, our proteomic approach provides novel pathways indicating that ENT1-regulated signaling is essential for neurotransmitter release and neuropeptide processing, both of which underlie learning and reward-seeking behaviors.
authors.affiliation: Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic , Rochester, Minnesota 55905, United States.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Oliveros
authors.affiliation: Neurobiology of Disease Program, Mayo Clinic , Rochester, Minnesota 55905, United States.
authors.forename: Phillip
authors.initials: P
authors.lastname: Starski
authors.affiliation: Neurobiology of Disease Program, Mayo Clinic , Rochester, Minnesota 55905, United States.
authors.forename: Daniel
authors.initials: D
authors.lastname: Lindberg
authors.affiliation: Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic , Rochester, Minnesota 55905, United States.
authors.forename: Sun
authors.initials: S
authors.lastname: Choi
authors.affiliation: Proteomics Research Center, Mayo Clinic , Rochester, Minnesota 55905, United States.
authors.forename: Carrie J
authors.initials: CJ
authors.lastname: Heppelmann
authors.affiliation: Division of Biomedical Statistics and Informatics, Mayo Clinic , Rochester, Minnesota 55905, United States.
authors.forename: Surendra
authors.initials: S
authors.lastname: Dasari
authors.affiliation: Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic , Rochester, Minnesota 55905, United States.|Neurobiology of Disease Program, Mayo Clinic , Rochester, Minnesota 55905, United States.|Department of Psychiatry and Psychology, Mayo Clinic , Rochester, Minnesota 55905, United States.
authors.forename: Doo-Sup
authors.identifier: 0000-0002-6796-9938
authors.initials: DS
authors.lastname: Choi
mesh_terms: D000428:Alcohol Drinking / Q000235:genetics / Q000473:pathology; D000818:Animals; D004745:Enkephalins / Q000235:genetics*; D033721:Equilibrative Nucleoside Transporter 1 / Q000235:genetics*; D000431:Ethanol / Q000378:metabolism; D006624:Hippocampus / Q000378:metabolism / Q000473:pathology; D017774:Long-Term Potentiation / Q000235:genetics; D051379:Mice; D009479:Neuropeptides / Q000096:biosynthesis / Q000235:genetics; D018377:Neurotransmitter Agents / Q000096:biosynthesis / Q000235:genetics; D009714:Nucleus Accumbens / Q000378:metabolism / Q000473:pathology; D045664:Proprotein Convertase 1 / Q000235:genetics*; D011498:Protein Precursors / Q000235:genetics*; D040901:Proteomics; D015398:Signal Transduction / Q000235:genetics; D053719:Tandem Mass Spectrometry; D050987:Vesicle-Associated Membrane Protein 1 / Q000235:genetics*
chemical_list: D004745:Enkephalins; D033721:Equilibrative Nucleoside Transporter 1; D009479:Neuropeptides; D018377:Neurotransmitter Agents; D011498:Protein Precursors; C498476:SLC29A1 protein, mouse; D050987:Vesicle-Associated Membrane Protein 1; C494771:vesicle-associated membrane protein 1, mouse; D000431:Ethanol; C033660:preproenkephalin; C473656:Pcsk1 protein, mouse; D045664:Proprotein Convertase 1
keywords: ENT1; adenosine; hippocampus; neuroproteomics; nucleus accumbens