title: Comparative Analysis of Transcriptional Profile Changes in Larval Zebrafish Exposed to Zinc Oxide Nanoparticles and Zinc Sulfate.
abstract: Many studies of the toxic effects of zinc oxide nanoparticles (ZnO NPs) in aquatic organisms have been performed because of increasing ZnO NP use. However, the toxicological pathways are not understood. In this study, ZnO NPs were found to be more toxic than ZnSO4to zebrafish larvae, but ZnO NP toxicity did not involve transcript alterations. Biological processes affected by ZnO NPs and ZnSO4were investigated by performing ingenuity pathway analysis on differently expressed genes in larvae exposed to sub-lethal ZnO NP and ZnSO4concentrations. We identified upregulated and downregulated differently expressed genes in fish exposed to ZnO NPs and ZnSO4, and found that ZnO NPs slightly induced cell differentiation and pathways associated with the immune system and activated several key genes involved in cancer cell signaling. The results may be key to predicting and elucidating the mechanisms involved in ZnO NP and ZnSO4toxicity in zebrafish larvae.
authors.affiliation: System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, 34111, South Korea.
authors.forename: Ryeo-Ok
authors.initials: RO
authors.lastname: Kim
authors.affiliation: Future Environmental Research Center, Korea Institute of Toxicology, Jinju, 52834, South Korea.
authors.forename: Jin Soo
authors.initials: JS
authors.lastname: Choi
authors.affiliation: System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, 34111, South Korea.
authors.forename: Byoung-Chul
authors.initials: BC
authors.lastname: Kim
authors.affiliation: System Toxicology Research Center, Korea Institute of Toxicology, Daejeon, 34111, South Korea. wookkim@kitox.re.kr.
authors.forename: Woo-Keun
authors.identifier: http://orcid.org/0000-0001-8355-4057
authors.initials: WK
authors.lastname: Kim
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects / Q000235:genetics; D004781:Environmental Exposure; D005786:Gene Expression Regulation / Q000187:drug effects; D007814:Larva / Q000187:drug effects / Q000235:genetics; D053758:Nanoparticles / Q000633:toxicity*; D015398:Signal Transduction / Q000187:drug effects; D015027:Zebrafish / Q000235:genetics*; D015034:Zinc Oxide / Q000633:toxicity*; D019287:Zinc Sulfate / Q000633:toxicity*
chemical_list: D019287:Zinc Sulfate; D015034:Zinc Oxide
keywords: Ingenuity pathway analysis; Microarray; Zebrafish; Zinc oxide nanoparticles