title: Nicotine versus 6-hydroxy-l-nicotine against chlorisondamine induced memory impairment and oxidative stress in the rat hippocampus.
abstract: 6-Hydroxy-l-nicotine (6HLN), a nicotine derivative from nicotine degradation by Arthrobacter nicotinovorans pAO1 strain was found to improve behavioral deficits and to reverse oxidative stress in the rat hippocampus. Rats were given CHL (10mg/kg, i.p.) were used as an Alzheimer's disease-like model. The nicotine (0.3mg/kg) and 6HLN (0.3mg/kg) were administered alone or in combination in the CHL-treated rats. Memory-related behaviors were evaluated using Y-maze and radial arm-maze tests. The antioxidant enzymes activity and the levels of the biomarkers of oxidative stress were measured in the hippocampus. Statistical analyses were performed using two-way ANOVA and Tukey's post hoc test. F values for which p<0.05 were regarded as statistically significant. CHL-caused memory deficits and oxidative stress enhancing were observed. Both nicotine and 6HLN administration attenuated the cognitive deficits and recovered the antioxidant capacity in the rat hippocampus of the CHL rat model. Our results suggest that 6HLN versus nicotine confers anti-amnesic properties in the CHL-induced a rat model of memory impairment via reversing cholinergic function and decreasing brain oxidative stress, suggesting the use of this compound as an alternative agent in AD treatment.
authors.affiliation: Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania. Electronic address: hritcu@uaic.ro.
authors.forename: Lucian
authors.initials: L
authors.lastname: Hritcu
authors.affiliation: Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Ionita
authors.affiliation: Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania.
authors.forename: Diana Elena
authors.initials: DE
authors.lastname: Motei
authors.affiliation: Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania.
authors.forename: Cornelia
authors.initials: C
authors.lastname: Babii
authors.affiliation: Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania.
authors.forename: Marius
authors.initials: M
authors.lastname: Stefan
authors.affiliation: Department of Biology, Alexandru Ioan Cuza University of Iasi, Bd. Carol I, No. 11, 700506, Romania. Electronic address: marius.mihasan@uaic.ro.
authors.forename: Marius
authors.initials: M
authors.lastname: Mihasan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology / Q000627:therapeutic use; D002714:Chlorisondamine / Q000633:toxicity*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D018782:Maze Learning / Q000187:drug effects / Q000502:physiology; D008569:Memory Disorders / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism; D009538:Nicotine / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use*; D018733:Nicotinic Antagonists / Q000633:toxicity; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: C502317:6-hydroxynicotine; D000975:Antioxidants; D018733:Nicotinic Antagonists; D009538:Nicotine; D002714:Chlorisondamine
keywords: 6-Hydroxy-l-nicotine; Arthrobacter nicotinovorans; Chlorisondamine; Memory; Nicotine; Oxidative stress