title: The impact of postnatal leuprolide acetate treatment on reproductive characteristics in a rodent model of polycystic ovary syndrome.
abstract: In this study, a GnRH agonist, leuprolide acetate (LA), was given as a single depot injection before 48 h of life to Wistar female rats allotted to prenatal (E16-18) and postnatal androgenization (day 5 of life) by the use of testosterone propionate, looking for reproductive endpoints. Remarkably, a single injection of LA increased the estrus cycles in the postnatal group (PostN) from 0% to 25% of the estrus cycles in the postnatal LA treated group (PostN L). LA also reduced the serum testosterone levels and cysts and atretic follicles in PostN L in contrast with rats (>100 days) from the PostN group (p = 0.04). Prenatally androgenized rats (PreN) exhibited significant modifications in the hypothalamic genes, such as Gnrh. To the best of our knowledge, this is the first study to show that blockage of the GnRH axis with leuprolide acetate depot prevented the development of typical features (anovulation, cysts, atretic follicles) in a postnatal testosterone propionate rat model of PCOS.
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Lady Katerine
authors.initials: LK
authors.lastname: Serrano Mujica
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Kalyne
authors.initials: K
authors.lastname: Bertolin
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Bridi
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Werner Giehl
authors.initials: WG
authors.lastname: Glanzner
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Vitor Braga
authors.initials: VB
authors.lastname: Rissi
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Flávia de Los Santos
authors.initials: FL
authors.lastname: de Camargo
authors.affiliation: Laboratory of Pesticide Residue Analysis-LARP, Chemistry Department, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Renato
authors.initials: R
authors.lastname: Zanella
authors.affiliation: Laboratory of Pesticide Residue Analysis-LARP, Chemistry Department, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Osmar Damian
authors.initials: OD
authors.lastname: Prestes
authors.affiliation: Laboratory of Clinical Biochemistry, Department of Clinical and Toxicological Analysis, Federal University of Santa Maria, Santa Maria, RS, Brazil.
authors.forename: Rafael Noal
authors.initials: RN
authors.lastname: Moresco
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Alfredo Quites
authors.initials: AQ
authors.lastname: Antoniazzi
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Paulo Bayard
authors.initials: PB
authors.lastname: Dias Gonçalves
authors.affiliation: Department of Clinical Medicine, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Melissa Orlandin
authors.initials: MO
authors.lastname: Premaor
authors.affiliation: Laboratory of Biotechnology and Animal Reproduction - BioRep, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil; Department of Clinical Medicine, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: fcomim@ufsm.br.
authors.forename: Fabio Vasconcellos
authors.initials: FV
authors.lastname: Comim
mesh_terms: D000818:Animals; D000858:Anovulation / Q000188:drug therapy / Q000378:metabolism; D030762:Estrous Cycle / Q000187:drug effects; D005260:Female; D007987:Gonadotropin-Releasing Hormone / Q000378:metabolism; D016729:Leuprolide / Q000494:pharmacology*; D008297:Male; D006080:Ovarian Follicle / Q000378:metabolism; D011085:Polycystic Ovary Syndrome / Q000188:drug therapy* / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012098:Reproduction / Q000187:drug effects*; D013739:Testosterone / Q000378:metabolism; D014770:Virilism / Q000188:drug therapy / Q000378:metabolism
chemical_list: D007987:Gonadotropin-Releasing Hormone; D013739:Testosterone; D016729:Leuprolide
keywords: Developmental programming; Leuprolide acetate; Polycystic ovary syndrome