title: Comparison of the antimicrobial activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine.
abstract: OBJECTIVE
  To compare the antimicrobial activity of the tricalcium silicate-based Biodentine (BD) and mineral trioxide aggregate (MTA)-Angelus cement with the aid of agar diffusion test.
  
  
  METHODS
  Staphylococcus aureus,Pseudomonas aeruginosa,Escherichia coli, andEnterococcus faeciumwere inoculated in theBrucellaliquid medium and were incubated at 37°C for 24 h. Thereafter, 100 >μl of the liquid culture of bacteria inoculated in the Mueller-Hinton agar with spread plate technique. Petri plates were dried in room temperature. For every microorganism, 3 petri plates were prepared (12 in total). In the medium, in every petri plate, 2 holes with 5 mm diameter and 2 mm depth were made. Afterward, BD and MTA-Angelus were filled into these holes under aseptic conditions according to the instructions of the manufacturing company. Then, the plates were kept in the incubator at 37°C for 24 h, and the diameters of the inhibition zones were measured with a digital caliper.
  
  
  RESULTS
  Inhibition zones formed by BD againstE. coliandS. aureuswere significantly larger than the zones formed by MTA-Angelus (P< 0.05). However, the inhibition zones formed by MTA-Angelus againstP. aureusandE. faeciumwere larger than the zones formed by BD (P< 0.05).
  
  
  CONCLUSIONS
  Within the limits of the present study, tricalcium silicate-based MTA-Angelus and BD have antimicrobial activity againstE. coli,S. aureus,P. aureus, andE. faecium.
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Taha
authors.initials: T
authors.lastname: Özyürek
authors.affiliation: Department of Endodontics, Faculty of Dentistry, Ondokuz Mayis University, Samsun, Turkey.
authors.forename: Ebru Özsezer
authors.initials: EÖ
authors.lastname: Demiryürek
keywords: Agar diffusion test; Biodentine; direct pulp capping; endodontics; mineral trioxide aggregate-Angelus