title: MiR-520b/e Regulates Proliferation and Migration by Simultaneously Targeting EGFR in Gastric Cancer.
abstract: BACKGROUND
  MicroRNAs (miRNAs) have been demonstrated to play a crucial role in tumorigenesis. Previous studies have shown that miR-520b/e acts as a tumor suppressor in several tumors. Other studies indicated that epidermal growth factor receptor (EGFR) is highly expressed in many tumors, and involved in the development of tumors, such as cell proliferation, migration, angiogenesis and apoptosis. However, the correlation of miRNAs and EGFR in gastric cancer (GC) has not been adequately investigated. Our aim was to explore the relationship.
  
  
  METHODS
  The expression levels of EGFR and miR-520b/e were examined by RT-PCR and Western blot. We also investigated the relationship between EGFR and miR-520b/e in GC cell lines by relevant experiments.
  
  
  RESULTS
  In this study, we found that miR-520b/e inhibits the protein expression of EGFR by directly binding with the 3'-untranslated region (3'-UTR). And it was shown that the down-regulation of miR-520b/e promotes cell proliferation and migration by negative regulation of the EGFR pathway, while over-expression of miR-520b/e inhibits these properties. In addition, the biological function of EGFR in GC cell lines was validated by silencing and over-expression assays respectively.
  
  
  CONCLUSIONS
  Taken together, our results demonstrate that miR-520b/e acts as a tumor suppressor by regulating EGFR in GC, and provide a novel marker and insight for the potential therapeutic target of GC.
authors.affiliation: Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.forename: Haiyang
authors.initials: H
authors.lastname: Zhang
authors.forename: Tao
authors.initials: T
authors.lastname: Ning
authors.forename: Xinyi
authors.initials: X
authors.lastname: Wang
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.forename: Haiou
authors.initials: H
authors.lastname: Yang
authors.forename: Yueting
authors.initials: Y
authors.lastname: Han
authors.forename: Ting
authors.initials: T
authors.lastname: Deng
authors.forename: Likun
authors.initials: L
authors.lastname: Zhou
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.forename: Ming
authors.initials: M
authors.lastname: Bai
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.forename: Shaohua
authors.initials: S
authors.lastname: Ge
authors.forename: Guoguang
authors.initials: G
authors.lastname: Ying
authors.forename: Yi
authors.initials: Y
authors.lastname: Ba
mesh_terms: D001483:Base Sequence; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics*; D049109:Cell Proliferation; D015536:Down-Regulation / Q000235:genetics; D066246:ErbB Receptors / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C575048:MIRN520 microRNA, human; D035683:MicroRNAs; D008156:Luciferases; C512478:EGFR protein, human; D066246:ErbB Receptors