title: KIF2A regulates the spindle assembly and the metaphase I-anaphase I transition in mouse oocyte.
abstract: KIF2A, a member of the kinesin-13 family, has been reported to play a role in spindle assembly in mitosis. However, its function in mammalian meiosis remains unknown. In this research, we examined the expression, localization and function of KIF2A during mouse oocyte meiosis. KIF2A was expressed in some key stages in mouse oocyte meiosis. Immunofluorescent staining showed that KIF2A distributed in the germinal vesicle at the germinal vesicle stage and as the spindle assembling after meiosis resumption, KIF2A gradually accumulated to the entire spindle. The treatment of oocytes with taxol and nocodazole demonstrated that KIF2A was co-localized with α-tubulin. Depletion of KIF2A by specific short interfering (si) RNA injection resulted in abnormal spindle assembly, failure of spindle migration, misaligned chromosomes and asymmetric cell division. Meanwhile, SKA1 expression level was decreased and the TACC3 localization was disrupted. Moreover, depletion of KIF2A disrupted the actin cap formation, arrested oocytes at metaphase I with spindle assembly checkpoint protein BubR1 activated and finally reduced the rate of the first polar body extrusion. Our data indicate that KIF2A regulates the spindle assembly, asymmetric cytokinesis and the metaphase I-anaphase I transition in mouse oocyte.
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Ming-Huang
authors.initials: MH
authors.lastname: Chen
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Ya-Long
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.|Department of Gynaecology and Obstetrics, Zhongxin Hospital, Qingdao 266000, Shandong, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Liu
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Bai-Hui
authors.initials: BH
authors.lastname: Xu
authors.affiliation: Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.|Biological College, Southwest Forestry University, Kunming 650000, Yunnan, China.
authors.forename: Fei-Ping
authors.initials: FP
authors.lastname: Li
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Xu
authors.affiliation: Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.|Department of Gynaecology and Obstetrics, The First Clinical Medical College, Fujian Medical University, Fuzhou 350000, Fujian, China.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Lin
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Shu-Wen
authors.initials: SW
authors.lastname: He
authors.affiliation: Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.|Department of Gynaecology and Obstetrics, Dongfang Hospital, Xiamen University, Fuzhou 350000, Fujian, China.
authors.forename: Bao-Qiong
authors.initials: BQ
authors.lastname: Liao
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Xian-Pei
authors.initials: XP
authors.lastname: Fu
authors.affiliation: Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen 361000, Fujian, China.
authors.forename: Xiao-Xue
authors.initials: XX
authors.lastname: Wang
authors.affiliation: Department of Gynaecology and Obstetrics, Zhongshan Hospital, Xiamen University, Xiamen 361000, Fujian, China.
authors.forename: Xiang-Jun
authors.initials: XJ
authors.lastname: Yang
authors.affiliation: Organ Transplantation Institute, Medical College, Xiamen University, Xiamen 361000, Fujian, China.|Fujian Key Laboratory of Organ and Tissue Regeneration, Xiamen 361000, Fujian, China.
authors.forename: Hai-Long
authors.initials: HL
authors.lastname: Wang
mesh_terms: D000705:Anaphase; D000818:Animals; D020869:Gene Expression Profiling; D016547:Kinesins / Q000378:metabolism*; D008677:Metaphase; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D012097:Repressor Proteins / Q000378:metabolism*; D008941:Spindle Apparatus
chemical_list: D012097:Repressor Proteins; C477238:KIF2A protein, mouse; D016547:Kinesins