title: Detection of Reactive Oxygen Species by a Carbon-Dot-Ascorbic Acid Hydrogel.
abstract: Detection of reactive oxygen species (ROS) is important in varied biological processes, disease diagnostics, and chemotherapeutic drug screening. We constructed a ROS sensor comprising an ascorbic-acid-based hydrogel encapsulating luminescent amphiphilic carbon-dots (C-dots). The sensing mechanism is based upon ROS-induced oxidation of the ascorbic acid units within the hydrogel scaffold; as a consequence, the hydrogel framework collapses resulting in aggregation of the C-dots and quenching of their luminescence. The C-dot-hydrogel platform exhibits high sensitivity and detected ROS generated chemically in solution and in actual cell environments. We demonstrate application of the C-dot-hydrogel for evaluating the efficacy of a chemotherapeutic substance, underscoring the potential of the system for drug screening applications.
authors.affiliation: Department of Chemistry, Ben-Gurion University of the Negev , Beer Sheva 84105, Israel.
authors.forename: Sagarika
authors.initials: S
authors.lastname: Bhattacharya
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva 84105, Israel.
authors.forename: Rhitajit
authors.initials: R
authors.lastname: Sarkar
authors.affiliation: Department of Chemistry, Ben-Gurion University of the Negev , Beer Sheva 84105, Israel.
authors.forename: Sukhendu
authors.initials: S
authors.lastname: Nandi
authors.affiliation: The Shraga Segal Department of Microbiology, Immunology and Genetics, Faculty of Health Sciences, Ben-Gurion University of the Negev , Beer Sheva 84105, Israel.
authors.forename: Angel
authors.initials: A
authors.lastname: Porgador
authors.affiliation: Department of Chemistry, Ben-Gurion University of the Negev , Beer Sheva 84105, Israel.|Ilse Katz Institute for Nanotechnology, Ben-Gurion University of the Negev , Beer Sheva 84105, Israel.
authors.forename: Raz
authors.identifier: 0000-0002-0336-1384
authors.initials: R
authors.lastname: Jelinek
mesh_terms: D001205:Ascorbic Acid / Q000737:chemistry*; D002244:Carbon / Q000737:chemistry*; D016923:Cell Death; D005434:Flow Cytometry; D006367:HeLa Cells; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008968:Molecular Conformation; D017382:Reactive Oxygen Species / Q000032:analysis* / Q000378:metabolism; D013050:Spectrometry, Fluorescence; D014407:Tumor Cells, Cultured
chemical_list: D017382:Reactive Oxygen Species; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D002244:Carbon; D001205:Ascorbic Acid