title: A simple and rapid LC-MS/MS method for quantitation of luseogliflozin in rat plasma and its application to a PK study.
abstract: OBJECTIVE
  Luseogliflozin is a novel sodium-dependent glucose cotransporter-2 inhibitor for the treatment of Type 2 diabetes mellitus. To assist pharmacokinetic and toxicodynamic studies, a rapid LC-MS/MS method were developed and validated for the quantitation of luseogliflozin in rat plasma.
  
  
  RESULTS
  Sample preparation was carried out using simplified protein precipitation and liquid-liquid extraction procedures, and the run time was only 4 min. Extraction recovery was 92.9 to 95.3%, and the method was validated over the range 0.5 to 500 ng/ml for luseogliflozin with acceptable specificity, accuracy and precision.
  
  
  CONCLUSIONS
  The validated method is considered suitable to quantify luseogliflozin in pharmacokinetic and pharmacodynamic/toxicodynamic studies in rats.
authors.affiliation: Department of Pharmacokinetics, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Kobuchi
authors.affiliation: Department of Pharmacokinetics, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Matsuno
authors.affiliation: Department of Pharmacokinetics, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Momoko
authors.initials: M
authors.lastname: Kawamoto
authors.affiliation: Department of Pharmaceutical Manufacturing Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Naoto
authors.initials: N
authors.lastname: Kojima
authors.affiliation: Department of Pharmacokinetics, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Yukako
authors.initials: Y
authors.lastname: Ito
authors.affiliation: Department of Pharmaceutical Manufacturing Chemistry, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Masayuki
authors.initials: M
authors.lastname: Yamashita
authors.affiliation: Department of Pharmacokinetics, Kyoto Pharmaceutical University, Kyoto 607-8414, Japan.
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Sakaeda
mesh_terms: D000818:Animals; D001774:Blood Chemical Analysis / Q000295:instrumentation / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D004355:Drug Stability; D006207:Half-Life; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D051297:Sodium-Glucose Transporter 2 / Q000378:metabolism; D000077203:Sodium-Glucose Transporter 2 Inhibitors; D013012:Sorbitol / Q000031:analogs & derivatives* / Q000097:blood / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry; D013696:Temperature
chemical_list: D051297:Sodium-Glucose Transporter 2; D000077203:Sodium-Glucose Transporter 2 Inhibitors; D013012:Sorbitol; C549343:1,5-anhydro-1-(5-(4-ethoxybenzyl)-2-methoxy-4-methylphenyl)-1-thioglucitol
keywords: LC–MS/MS; SGLT2 inhibitors; antidiabetic agent; dapagliflozin; luseogliflozin; pharmacokinetics; rat plasma