title: Graphene oxide directed in-situ deposition of electroactive silver nanoparticles and its electrochemical sensing application for DNA analysis.
abstract: The development of high-performance biosensing platform is heavily dependent on the recognition property of the sensing layer and the output intensity of the signal probe. Herein, we present a simple and highly sensitive biosensing interface for DNA detection on the basis of graphene oxide nanosheets (GONs) directed in-situ deposition of silver nanoparticles (AgNPs). The fabrication process and electrochemical properties of the biosensing interface were probed by electrochemical techniques and scanning electron microscopy. The results indicate that GONs can specifically adsorb at the single-stranded DNA probe surface, and induces the deposition of highly electroactive AgNPs. Upon hybridization with complementary oligonucleotides to generate the duplex DNA on the electrode surface, the GONs with the deposited AgNPs will be liberated from the sensing interface due to the inferior affinity of GONs and duplex DNA, resulting in the reduction of the electrochemical signal. Such a strategy combines the superior recognition of GONs toward single-stranded DNA and double-stranded DNA, and the strong electrochemical response of in-situ deposited AgNPs. Under optimal conditions, the biosensor can detect target DNA over a wide range from 10 fM to 10 nM with a detection limit of 7.6 fM. Also, the developed biosensor shows outstanding discriminating ability toward oligonucleotides with different mismatching degrees.
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Gao
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China; Department of Chemistry, Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu, Matsue, Shimane, 690-8504, Japan. Electronic address: fgao1981@mnnu.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China.
authors.forename: Suyu
authors.initials: S
authors.lastname: He
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China.
authors.forename: Qionghua
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China.
authors.forename: Jiafu
authors.initials: J
authors.lastname: Huang
authors.affiliation: Department of Chemistry, Graduate School of Science and Engineering, Shimane University, 1060 Nishikawatsu, Matsue, Shimane, 690-8504, Japan.
authors.forename: Hidekazu
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: College of Chemistry and Environment, Fujian Province Key Laboratory of Modern Analytical Science and Separation Technology, Minnan Normal University, Zhangzhou, 363000, PR China. Electronic address: axiang236@126.com.
authors.forename: Qingxiang
authors.initials: Q
authors.lastname: Wang
mesh_terms: D015374:Biosensing Techniques; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles; D010087:Oxides / Q000737:chemistry; D012834:Silver
chemical_list: D010087:Oxides; D012834:Silver; D006108:Graphite; D004247:DNA
keywords: DNA hybridization; Electrochemical sensor; Graphene oxide nanosheets; In-situ deposition; Silver nanoparticles