title: A green and facile approach for synthesizing imine to develop optical biosensor for wide range detection of bilirubin in human biofluids.
abstract: Bilirubin, a key biomarker for the jaundice and its clinical diagnosis needs a better analytical tool. A novel and simple fluorescent platform based on (2,2'-((1E,1'E)-((6-bromopyridine-2,3-diyl) bis(azanylylidene)) bis(methanylylidene diphenol) (BAMD) was designed. BAMD showed a remarkable fluorescent intensity with a very good quantum yield of 0.85 and lifetime of 870ps. Hence, it was applied for the determination of bilirubin using both colorimetric and fluorimetric techniques in physiological and basic pH. Under optimized experimental conditions, the probe detects bilirubin selectively in the presence of other interfering biomolecules and metal ions. The linear range of detection is 1pM-500µM at pH=7.4 and LOD is 2.8 and 3.3 pM at pH=7.4 and 9.0, respectively, which were reported so far. The probe detects the bilirubin through FRET mechanism. The practical application of the probe was successfully tested in the human blood and urine samples. Based on all above advantages, this simple idea can be applied to design a simple clinical diagnostic tool for jaundice.
authors.affiliation: Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
authors.forename: Sundaram
authors.initials: S
authors.lastname: Ellairaja
authors.affiliation: Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India.
authors.forename: Kathiravan
authors.initials: K
authors.lastname: Shenbagavalli
authors.affiliation: Biotechnology Division, Defence Research Development & Establishment, Jhansi Road, Gwalior 474002, Madhya Pradesh, India.
authors.forename: Sarkaraisamy
authors.initials: S
authors.lastname: Ponmariappan
authors.affiliation: Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai 625021, Tamil Nadu, India. Electronic address: vasantham999@yahoo.co.in.
authors.forename: Vairathevar Sivasamy
authors.initials: VS
authors.lastname: Vasantha
mesh_terms: D000631:Aminopyridines / Q000737:chemistry*; D001663:Bilirubin / Q000097:blood* / Q000652:urine*; D015374:Biosensing Techniques / Q000379:methods*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: C000616262:2,2'-(((6-bromopyridine-2,3-diyl) bis(azanylylidene)) bis(methanylylidene diphenol); D000631:Aminopyridines; D005456:Fluorescent Dyes; D001663:Bilirubin
keywords: Bilirubin, 2,2′-((1E,1′E)-((6-bromopyridine-2,3-diyl) bis(azanylylidene)) bis(methanylylidene)) diphenol; Colorimetric biosensor; Competitive study; Fluorimetry biosensor; Imine molecule; Lifetime study; Sonochemical synthesis