title: N-RAS expression in patients with salivary adenoid cystic carcinoma: association with clinicopathologic features and prognosis.
abstract: OBJECTIVE
  The aim of this study was to investigate N-RAS expression in patients with salivary adenoid cystic carcinoma (SACC) and to analyze its association with clinicopathologic features and prognosis.
  
  
  METHODS
  We determined N-RAS expression in SACC tumors via immunohistochemistry and evaluated the association between N-RAS expression and clinicopathologic variables. The disease-free survival (DFS) curves were plotted by using the Kaplan-Meier method. The parameters of prognostic significance found by univariate analysis were tested by a multivariate Cox regression model.
  
  
  RESULTS
  N-RAS expression was detected in 61 of 72 SACC tumor samples (84.7%). In univariate analyses, N-RAS expression was associated with clinical stage, tumor size, adjacent tissue invasion, and distant metastasis. N-RAS expression was associated with DFS in univariate analysis; however, in multivariate analysis, N-RAS was not an independent prognostic indicator, but clinical stage (0.04) and histologic subtype (0.02) were statistically significant.
  
  
  CONCLUSIONS
  Our data suggest that N-RAS was overexpressed in SACC tissues, but was not an independent prognostic indicator of DFS. Only clinical stage and histologic subtype were prognostic indicators of DFS.
authors.affiliation: Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
authors.forename: Jiuling
authors.initials: J
authors.lastname: Yue
authors.affiliation: Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhuo
authors.affiliation: Department of Otorhinolaryngology and Maxillofacial Oncology, Tianjin Medical University Cancer Institute & Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin, China. Electronic address: Zwbeyond_999@sina.com.
authors.forename: Wenchao
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D003528:Carcinoma, Adenoid Cystic / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D018572:Disease-Free Survival; D005260:Female; D020558:GTP Phosphohydrolases / Q000378:metabolism*; D006801:Humans; D007150:Immunohistochemistry; D008297:Male; D008565:Membrane Proteins / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011379:Prognosis; D012468:Salivary Gland Neoplasms / Q000378:metabolism* / Q000473:pathology / Q000628:therapy
chemical_list: D008565:Membrane Proteins; D020558:GTP Phosphohydrolases; C579846:NRAS protein, human