title: Description and phylogeny of a new microsporidium from the elm leaf beetle, Xanthogaleruca luteola Muller, 1766 (Coleoptera: Chrysomelidae).
abstract: This study describes a new genus and species of microsporidia which is a pathogen of the elm leaf beetle, Xanthogaleruca luteola Muller, 1776 (Coleoptera: Chrysomelidae). The beetles were collected from Istanbul in Turkey. All developmental stages are uninucleate and in direct contact with the host cell cytoplasm. Giemsa-stained mature spores are oval in shape and measured 3.40 ± 0.37 μm in length and 1.63 ± 0.20 μm in width. These uninucleate spores have an isofilar polar filament with 11 turns. The spore wall was trilaminar (75 to 115 nm) with a rugose, electron-dense exospore (34 to 45 nm) and a thickened, electron-lucent endospore (65 to 80 nm) overlaying the plasmalemma. Morphological, ultrastructural, and molecular features indicate that the described microsporidium is dissimilar to all known microsporidian taxa and confirm that it has different taxonomic characters than other microsporidia infecting X. luteola and is named here as Rugispora istanbulensis n. gen., n. sp.
authors.affiliation: Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey. cagribekircan@hotmail.com.
authors.forename: Çağrı
authors.initials: Ç
authors.lastname: Bekircan
authors.affiliation: Department of Biology, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Ufuk
authors.initials: U
authors.lastname: Bülbül
authors.affiliation: Department of Molecular Biology and Genetic, Faculty of Sciences, Karadeniz Technical University, 61080, Trabzon, Turkey.
authors.forename: Halil I
authors.initials: HI
authors.lastname: Güler
authors.affiliation: United States Department of Agriculture, Agriculture Research Service, Center for Medical, Agricultural and Veterinary Entomology (CMAVE), 1600 SW 23rd Drive, Gainesville, FL, USA.
authors.forename: James J
authors.initials: JJ
authors.lastname: Becnel
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000382:microbiology*; D021861:Microsporidia, Unclassified / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000648:ultrastructure; D010802:Phylogeny; D018515:Plant Leaves / Q000469:parasitology; D017422:Sequence Analysis, DNA; D013172:Spores, Fungal; D014421:Turkey; D034403:Ulmus / Q000469:parasitology
keywords: Coleoptera; Elm; Microsporidia; Pathogen; Ultrastructure