title: Angiogenesis Inhibitors and Anti-Inflammatory Agents from Phoma sp. NTOU4195.
abstract: Seven new polyketides, phomaketides A-E (1-5) and pseurotins A3(6) and G (7), along with the known compounds FR-111142, pseurotins A, A1, A2, D, and F2, 14-norpseurotin A, α-carbonylcarbene, tyrosol, cyclo(-l-Pro-l-Leu), and cyclo(-l-Pro-l-Phe), were purified from the fermentation broth and mycelium of the endophytic fungal strain Phoma sp. NTOU4195 isolated from the marine red alga Pterocladiella capillacea. The structures were established through interpretation of spectroscopic data. The antiangiogenic and anti-inflammatory effects of 1-7 and related analogues were evaluated using human endothelial progenitor cells (EPCs) and lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells, respectively. Of the compounds tested, compound 1 exhibited the most potent antiangiogenic activity by suppressing the tube formation of EPCs with an IC50of 8.1 μM, and compound 3 showed the most selective inhibitory activity of LPS-induced NO production in RAW264.7 macrophages with an IC50value of 8.8 μM.
authors.forename: Ming-Shian
authors.initials: MS
authors.lastname: Lee
authors.affiliation: Department of Medicine, Mackay Medical College , New Taipei City 25245, Taiwan.
authors.forename: Shih-Wei
authors.initials: SW
authors.lastname: Wang
authors.affiliation: School of Medicine, Graduate Institute of Clinical Medical Science, China Medical University , Taichung 40402, Taiwan.|Department of Medical Research, China Medical University Hospital , Taichung 40447, Taiwan.|Department of Health and Nutrition Biotechnology, Asia University , Taichung 41354, Taiwan.
authors.forename: Guei-Jane
authors.initials: GJ
authors.lastname: Wang
authors.affiliation: Institute of Marine Biology and Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung 20224, Taiwan.
authors.forename: Ka-Lai
authors.initials: KL
authors.lastname: Pang
authors.forename: Ching-Kuo
authors.initials: CK
authors.lastname: Lee
authors.affiliation: Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University , Taichung 40447, Taiwan.|Department of Biotechnology, Asia University , Taichung 41354, Taiwan.
authors.forename: Yueh-Hsiung
authors.initials: YH
authors.lastname: Kuo
authors.affiliation: Institute of Marine Biology and Center of Excellence for the Oceans, National Taiwan Ocean University , Keelung 20224, Taiwan.
authors.forename: Hyo-Jung
authors.initials: HJ
authors.lastname: Cha
authors.forename: Ruo-Kai
authors.initials: RK
authors.lastname: Lin
authors.affiliation: Institute of Fisheries Science, National Taiwan University , Taipei 10617, Taiwan.
authors.forename: Tzong-Huei
authors.identifier: http://orcid.org/0000-0001-8036-7563
authors.initials: TH
authors.lastname: Lee
mesh_terms: D020533:Angiogenesis Inhibitors / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D001203:Ascomycota / Q000737:chemistry*; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D008070:Lipopolysaccharides / Q000494:pharmacology; D008264:Macrophages / Q000187:drug effects; D008386:Marine Biology; D051379:Mice; D015394:Molecular Structure; D009569:Nitric Oxide / Q000096:biosynthesis; D061065:Polyketides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D013141:Spiro Compounds / Q000494:pharmacology; D013624:Taiwan
chemical_list: C540632:14-norpseurotin; D020533:Angiogenesis Inhibitors; D000893:Anti-Inflammatory Agents; D004852:Epoxy Compounds; D008070:Lipopolysaccharides; D061065:Polyketides; D013141:Spiro Compounds; C074363:FR 111142; D009569:Nitric Oxide