title: MicroRNA-125b as a new potential biomarker on diagnosis of renal ischemia-reperfusion injury.
abstract: Acute renal failure is commonly seen in the perioperative period. Ischemia-reperfusion (IR) injury plays a major role in acute renal failure and delayed graft function. MicroRNAs (miRs), which are pivotal modulators of cell activities, offer a major opportunity for affective diagnosis and treatment strategies because they are tissue specific and in the center of gene expression modulation. The effect of bardoxolone methyl (BM) on miR-21, miR-223-5p, and miR-125b in renal IR injury was evaluated in this study.
  
  
  
  Wistar-Albino rats (12-16 wk old, weighing 300-350 g) were used in the study. Rats (n = 6) were randomized into three groups (control, IR, and BM + IR). Tissue levels of miRs were analyzed with reverse transcription polymerase chain reaction.
  
  
  
  Significant reduction of urea and total oxidant status, increase of total antioxidant status, and oxidative stress index were identified in the IR + BM group compared with the IR group. Significant increases of miR-21 (2842.82-fold) and miR-125b (536.8-fold) were identified in the IR group compared with the control group; however, miR-223-5p levels did not show any significant difference. Also, miR-21 and miR-125b were significantly reduced in the IR + BM group compared with the IR group. Reduced histopathologic changes were observed in the IR + BM group. A significant decrease in the number of tunel-positive cells was identified in the IR + BM group compared with the IR group.
  
  
  
  miR-125b was significantly increased in IR injury; thus, miR-125b can be a potential novel marker that can be used in diagnosis and treatment of renal IR injury. BM reduces miR-21 and miR-125b in case of IR injury and makes functional and histopathologic repairs.
authors.affiliation: Department of Nephrology, Ahi Evran School of Medicine, Kırşehir, Turkey. Electronic address: aydinguclu@gmail.com.
authors.forename: Aydın
authors.initials: A
authors.lastname: Güçlü
authors.affiliation: Department of Pathology, Dumlupınar School of Medicine, Kütahya, Turkey.
authors.forename: Cengiz
authors.initials: C
authors.lastname: Koçak
authors.affiliation: Department of Biochemistry, Dumlupınar School of Medicine, Kütahya, Turkey.
authors.forename: Fatma E
authors.initials: FE
authors.lastname: Koçak
authors.affiliation: Department of Physiology, Dumlupınar School of Medicine, Kütahya, Turkey.
authors.forename: Raziye
authors.initials: R
authors.lastname: Akçılar
authors.affiliation: Department of Medical Biology, Pamukkale School of Medicine, Denizli, Turkey.
authors.forename: Yavuz
authors.initials: Y
authors.lastname: Dodurga
authors.affiliation: Experimental Research Unit, Dumlupınar School of Medicine, Kütahya, Turkey.
authors.forename: Aydın
authors.initials: A
authors.lastname: Akçılar
authors.affiliation: Department of Medical Biology, Pamukkale School of Medicine, Denizli, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Elmas
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology* / Q000627:therapeutic use; D015415:Biomarkers / Q000378:metabolism; D007668:Kidney / Q000098:blood supply / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000378:metabolism*; D009828:Oleanolic Acid / Q000031:analogs & derivatives* / Q000494:pharmacology / Q000627:therapeutic use; D018384:Oxidative Stress / Q000187:drug effects; D011897:Random Allocation; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015427:Reperfusion Injury / Q000175:diagnosis* / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology
chemical_list: D000975:Antioxidants; D015415:Biomarkers; C581261:MIRN125 microRNA, rat; C000609258:MIRN223 microRNA, rat; D035683:MicroRNAs; C546082:mirn21 microRNA, rat; D009828:Oleanolic Acid; C445068:bardoxolone methyl
keywords: Bardoxolone methyl; CDDO-Me; MicroRNAs; Renal ischemia–reperfusion injury; miR-125b; miR-21; miR-223-5p