title: Effect of Non-Surgical Periodontal Treatment on Gingival Crevicular Fluid and Serum Endocan, Vascular Endothelial Growth Factor-A, and Tumor Necrosis Factor-Alpha Levels.
abstract: Periodontitis is a chronic inflammatory disease that occurs due to the interaction between pathogenic microorganisms and host defenses. Endocan is a proteoglycan secreted by endothelial cells under the control of inflammatory cytokines. Aims of the study are to determine serum and gingival crevicular fluid (GCF) endocan levels in the pathogenesis of periodontal diseases, supported with vascular endothelial growth factor (VEGF-A) and tumor necrosis factor (TNF)-alpha levels. This study additionally aims to evaluate correlation between GCF endocan levels, VEGF-A, and TNF-α levels with periodontal probing depth (PD).
  
  
  
  The study consists of two groups: group 1 (n = 20), healthy individuals; group 2 (n = 20), individuals with generalized chronic periodontitis (CP). Clinical measurements were recorded; GCF and serum samples were obtained from each participant before and 6 weeks after therapy. Levels of biomarkers were measured by enzyme-linked immunosorbent assay. Intergroup comparisons of biochemical and clinical parameters were analyzed by Kruskal-Wallis/Bonferroni-adjusted Mann-Whitney U test using statistical software.
  
  
  
  Serum and GCF endocan, VEGF-A, and TNF-α levels were significantly higher in patients with CP than in healthy individuals (P <0.001) and decreased after treatment (P <0.03). A significant correlation was observed between GCF TNF-α and PD (4 mm ≤ PD ≤5 mm and PD ≥6 mm). A significant relationship was found among GCF endocan and TNF-α, VEGF-A, CAL, and GI for all groups (P <0.05).
  
  
  
  Endocan and TNF-α levels, both in GCF and serum, increased from health to periodontitis and decreased with non-surgical periodontal treatment. Within the limits of the study, endocan may be considered as a potential inflammatory marker for periodontal disease.
authors.affiliation: Department of Periodontology, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: Çiğdem C
authors.initials: ÇC
authors.lastname: Türer
authors.affiliation: Department of Periodontology, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: Duygu
authors.initials: D
authors.lastname: Durmuş
authors.affiliation: Department of Periodontology, Bulent Ecevit University, Zonguldak, Turkey.
authors.forename: Umut
authors.initials: U
authors.lastname: Balli
authors.affiliation: Department of Medical Biochemistry, Bulent Ecevit University.
authors.forename: Berrak
authors.initials: B
authors.lastname: Güven
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D055113:Chronic Periodontitis / Q000628:therapy*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D005883:Gingival Crevicular Fluid / Q000737:chemistry*; D006801:Humans; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000032:analysis* / Q000097:blood; D011509:Proteoglycans / Q000032:analysis* / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis* / Q000097:blood; D042461:Vascular Endothelial Growth Factor A / Q000032:analysis* / Q000097:blood
chemical_list: C101134:ESM1 protein, human; D009363:Neoplasm Proteins; D011509:Proteoglycans; D014409:Tumor Necrosis Factor-alpha; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Aftercare; endocan proteins; gingival crevicular fluid; periodontal diseases; tumor necrosis factor-alpha; vascular endothelial growth factor a