title: Mature dendritic cell derived from cryopreserved immature dendritic cell shows impaired homing ability and reduced anti-viral therapeutic effects.
abstract: Cryopreservation is critical in reducing redundant operations and also in quality control in dendritic cell (DC) therapy. Full maturation and efficient homing of DCs to T cell-region constitute a crucial aspect of DC immunotherapy; however, the in vivo migration and distribution pattern, as well as the anti-viral effect of DCs that matured from cryopreserved immature DCs (cryoim-mDCs) remain to be revealed. In the present study, we compared cryoim-mDCs with DCs matured from fresh immature DCs (fmDCs) in the aspects of phenotypes, in vivo homing capacities as well as the anti-viral therapeutic effects to further clarify the effect of cryopreservation on DC-based cytotherapy. The results showed that cryopreservation impaired the homing ability of DCs which was associated with the reduced expression of CCR7 and disturbed cytoskeleton arrangement. Moreover, the antigen-specific CD8+ T cell response induced by cryoim-mDCs was much weaker than that induced by fmDCs in both the spleen and liver draining lymph nodes, which provided reduced protection from viral invasions. In conclusion, cryopreservation is a good method to keep the viability of immature DCs, however, the in vivo homing capacity and anti-viral therapeutic effect of DCs matured from frozen immature DCs were hindered to some extent.
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, P.R. China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, P.R. China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, P.R. China.
authors.forename: Man
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, P.R. China.|Key Laboratory of Advanced Energy Materials Chemistry (Ministry of Education), Nankai University, Tianjin 300071, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Public Health, Taishan Medical University, Taian, Shandong 271000, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Ma
authors.affiliation: School of Public Health, Taishan Medical University, Taian, Shandong 271000, China.
authors.forename: Xinquan
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Department of Blood Transfusion, PLA Army General Hospital, Beijing 100700, P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, P.R. China.
authors.forename: Donggen
authors.initials: D
authors.lastname: Wang
authors.affiliation: Beijing Institute of Transfusion Medicine, Beijing key Laboratory of Blood Safety and Supply Technologies, Beijing 100850, P.R. China.
authors.forename: Linsheng
authors.initials: L
authors.lastname: Zhan
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000494:pharmacology*; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology*; D002454:Cell Differentiation; D002465:Cell Movement; D002470:Cell Survival; D002478:Cells, Cultured; D015925:Cryopreservation; D003713:Dendritic Cells / Q000166:cytology* / Q000187:drug effects / Q000637:transplantation*; D007167:Immunotherapy; D008099:Liver / Q000276:immunology; D051379:Mice; D010641:Phenotype; D054400:Receptors, CCR7 / Q000378:metabolism; D013154:Spleen / Q000276:immunology
chemical_list: D000998:Antiviral Agents; C515371:Ccr7 protein, mouse; D054400:Receptors, CCR7