title: P1-independent replication and local movement of Rice yellow mottle virus in host and non-host plant species.
abstract: Sobemovirus P1 protein, characterized previously as a suppressor of posttranscriptional gene silencing, is required for systemic virus spread and infection in plants. Mutations in the ORF1 initiation codon do not affect viral replication indicating P1 is not necessary for this process. Wild type, recombinant and P1 deletion mutants of Cocksfoot mottle virus and Rice yellow mottle virus were used to infect oat, rice, wheat, barley, Arabidopsis thaliana and Nicotiana benthamiana plants. Wild type RYMV, RYMV without P1 and RYMV with CfMV P1 were detected in inoculated leaves of all tested plant species. We found that RYMV does not need P1 for replication and for local movement neither in host nor non-host species tested in this study. However, it is crucial for successful systemic spread of the virus in its host plant rice. Moreover, adding CfMV P1 into RYMV genome did not help it to overcome restriction to the inoculated leaf.
authors.affiliation: Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia. Electronic address: grete.nummert@gmail.com.
authors.forename: Grete
authors.initials: G
authors.lastname: Nummert
authors.affiliation: Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
authors.forename: Merike
authors.initials: M
authors.lastname: Sõmera
authors.affiliation: Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Uffert
authors.affiliation: Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
authors.forename: Erik
authors.initials: E
authors.lastname: Abner
authors.affiliation: Department of Gene Technology, Tallinn University of Technology, Akadeemia tee 15, 12618 Tallinn, Estonia.
authors.forename: Erkki
authors.initials: E
authors.lastname: Truve
mesh_terms: D001467:Hordeum / Q000821:virology; D058507:Host Specificity; D012275:Oryza / Q000821:virology; D010935:Plant Diseases / Q000821:virology*; D018515:Plant Leaves / Q000821:virology; D010942:Plant Viruses / Q000235:genetics / Q000502:physiology*; D012328:RNA Viruses / Q000235:genetics / Q000502:physiology*; D014026:Nicotiana / Q000821:virology; D014779:Virus Replication
keywords: Arabidopsis thaliana; Barley; Local movement; Nicotiana benthamiana; Oat; P1 gene; Rice; Sobemovirus; Virus replication; Wheat