title: Tween 80 and respiratory growth affect metabolite production and membrane fatty acids in Lactobacillus casei N87.
abstract: OBJECTIVE
  To evaluate the effect of cultivation (anaerobiosis vs respiration) and Tween 80 supplementation on the production of metabolites and on the composition of membrane fatty acids (FAs) in Lactobacillus casei N87.
  
  
  RESULTS
  Anaerobic and respiratory growth, with or without Tween 80 supplementation, was carried out in a chemically defined medium. Production of biomass, organic acids, volatile organic compounds (VOCs), consumption of amino acids and changes in membrane FAs were investigated. Respiration altered the central metabolism rerouting pyruvate away from lactate accumulation, while Tween 80 had a minor effect on metabolic pathways. VOCs were mainly affected by growth conditions and significant amounts of diacetyl were produced by respiratory cultures. Respiration increased desaturation of membrane lipids and Tween 80 improved the production of essential polyunsaturated FAs. Palmitic acid decreased in Tween-supplemented aerated cultures.
  
  
  CONCLUSIONS
  Combination of Tween 80 and respiratory growth promoted production of biomass and aroma compounds and affected the composition of membrane FAs in Lact. casei N87.
  
  
  CONCLUSIONS
  Respiration might be exploited in Lact. casei as a natural strategy for the enhanced production of aroma compounds.
authors.affiliation: Istituto di Scienze dell'Alimentazione-CNR, Avellino, Italy.
authors.forename: T
authors.initials: T
authors.lastname: Zotta
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Università degli Studi di Bologna, Sede di Cesena, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Tabanelli
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Università degli Studi di Bologna, Sede di Cesena, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Montanari
authors.affiliation: Scuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, Università degli Studi della Basilicata, Potenza, Italy.
authors.forename: R G
authors.initials: RG
authors.lastname: Ianniello
authors.affiliation: Dipartimento di Scienze, Università degli Studi della Basilicata, Potenza, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Parente
authors.affiliation: Centro Interdipartimentale di Ricerca Industriale Agroalimentare, Università degli Studi di Bologna, Sede di Cesena, Italy.|Dipartimento di Scienze e Tecnologie Agroalimentari, Alma Mater Studiorum, Università degli Studi di Bologna, Cesena, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gardini
authors.affiliation: Scuola di Scienze Agrarie, Forestali, Alimentari e Ambientali, Università degli Studi della Basilicata, Potenza, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Ricciardi
mesh_terms: D000332:Aerobiosis; D000596:Amino Acids / Q000378:metabolism; D000693:Anaerobiosis; D005227:Fatty Acids / Q000378:metabolism*; D019344:Lactic Acid / Q000378:metabolism; D007780:Lacticaseibacillus casei / Q000254:growth & development / Q000378:metabolism*; D053858:Metabolic Networks and Pathways / Q000187:drug effects; D011136:Polysorbates / Q000494:pharmacology*; D019289:Pyruvic Acid / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005227:Fatty Acids; D011136:Polysorbates; D019344:Lactic Acid; D019289:Pyruvic Acid
keywords: 
  Lactobacillus casei
  ; Tween 80; membrane fatty acids; respiration; volatile organic compounds