title: MicroRNA-322 inhibits inflammatory cytokine expression and promotes cell proliferation in LPS-stimulated murine macrophages by targeting NF-κB1 (p50).
abstract: Inflammation is the body's normal self-protection mechanism to eliminate pathogens and resist pathogen invasion. The excessive inflammatory response may lead to inflammatory lesions. The mechanisms accounting for inflammation remain hazy. miRNAs have been proposed to have crucial effects on inflammation. In the present study, we reported that lipopolysaccharide (LPS)-stimulation increased the expression levels of inflammatory cytokines and the cell-cycle progression was suppressed in RAW264.7 cells. Meanwhile, the expression of miR-322 was significantly down-regulated after LPS treatment. Bioinformatics predictions revealed a potential binding site of miR-322 in 3'-UTR of NF-κB1 (p50) and it was further confirmed by luciferase assay. Moreover, both the mRNA and protein levels of NF-κB1 (p50) were down-regulated by miR-322 in RAW264.7 cells. Subsequently, we demonstrated that miR-322 mimics decrease in the expression levels of inflammatory cytokines and cell-cycle repression can be rescued following LPS treatment in RAW264.7 cells. The anti-inflammatory cytokines expression including IL-4 and IL-10 were significantly up-regulated. Furthermore, miR-322 could also promote RAW264.7 cells proliferation. These results demonstrate that miR-322 is a negative regulator of inflammatory response by targeting NF-κB1 (p50).
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Song
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Lu
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Wenxun
authors.initials: W
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Lexing
authors.initials: L
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Danyang
authors.initials: D
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Shengbo
authors.initials: S
authors.lastname: Cao
authors.affiliation: College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China daihch@126.com.
authors.forename: Hanchuan
authors.initials: H
authors.lastname: Dai
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D002453:Cell Cycle; D049109:Cell Proliferation; D015536:Down-Regulation; D007249:Inflammation / Q000378:metabolism*; D016753:Interleukin-10 / Q000378:metabolism*; D015847:Interleukin-4 / Q000378:metabolism*; D008070:Lipopolysaccharides; D008264:Macrophages / Q000166:cytology / Q000378:metabolism*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D052002:NF-kappa B p50 Subunit / Q000378:metabolism*; D000067996:RAW 264.7 Cells; D012333:RNA, Messenger / Q000378:metabolism
chemical_list: D020413:3' Untranslated Regions; D008070:Lipopolysaccharides; C582672:MIRN322 microRNA, mouse; D035683:MicroRNAs; D052002:NF-kappa B p50 Subunit; D012333:RNA, Messenger; D016753:Interleukin-10; C073701:Nfkb1 protein, mouse; D015847:Interleukin-4
keywords: Inflammation; NF-κB1 (P50); miR-322