title: Multiplexed Phenotyping of Small Extracellular Vesicles Using Protein Microarray (EV Array).
abstract: The Extracellular Vesicle (EV) Array is based on the technology of protein microarray and provides the opportunity to detect and phenotype small EVs from unpurified starting material in a high-throughput manner (Jørgensen et al., J Extracell vesicles 2:1-9, 2013). The technology was established to perform multiplexed phenotyping of EVs in an open platform. This protocol outlines the microarray printing procedure followed by the steps of capture and detection of small extracellular vesicles from plasma/serum or cell culture supernatants. The principles of data treatment and analysis are thoroughly described as well.
authors.affiliation: Department of Clinical Immunology, Aalborg University Hospital, Urbansgade 36, 9000, Aalborg, Denmark.
authors.forename: Rikke
authors.initials: R
authors.lastname: Bæk
authors.affiliation: Department of Clinical Immunology, Aalborg University Hospital, Urbansgade 36, 9000, Aalborg, Denmark. maljoe@rn.dk.
authors.forename: Malene Møller
authors.initials: MM
authors.lastname: Jørgensen
mesh_terms: D015415:Biomarkers; D000067128:Extracellular Vesicles / Q000737:chemistry / Q000378:metabolism*; D010641:Phenotype; D040081:Protein Array Analysis / Q000379:methods*
chemical_list: D015415:Biomarkers
keywords: Capture antibodies; Exosomes; Extracellular vesicles; Multiplexed phenotyping; Protein microarray; Surface antigens