title: Molecular mechanism of lysozyme adsorption onto chemically modified alginate guar gum matrix.
abstract: The equilibrium isotherms and adsorption kinetics of lysozyme (LZ) on epichlorohydrin (Epi) cross-linked alginate-guar gum (Alg-GG) matrix were studied. Adsorption kinetics followed a pseudo-first-order model while the equilibrium isotherm could be represented by the Freundlich equation. The maximal amount of LZ adsorbed onto this matrix was around 2.4mg per g of hydrated matrix at pH 7.00. The adsorption mechanism was associated to a simple diffusion process with a weak columbic interaction between LZ and the matrix. The presence of NaCl 0.3M induced a total displacement of the LZ from the matrix. Under this condition, the percentage of desorbed protein was 95%. Successive cycles of adsorption-washing-elution were performed and the results showed the reversibility of the process and the usefulness of the method for enzyme purification and separation. A last successful step was carried out for the purification of LZ from egg white as natural source. The model proved to be useful applied as a platform design in the isolation and purification of proteins.
authors.affiliation: Institute of Biotechnological and Chemistry Processes, CONICET and Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 570, S2002RLK, Rosario, Argentina.
authors.forename: Ma Emilia
authors.initials: ME
authors.lastname: Brassesco
authors.affiliation: Institute of Biotechnological and Chemistry Processes, CONICET and Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 570, S2002RLK, Rosario, Argentina.
authors.forename: Nadia
authors.initials: N
authors.lastname: Woitovich Valetti
authors.affiliation: Institute of Biotechnological and Chemistry Processes, CONICET and Faculty of Biochemical and Pharmaceutical Sciences, National University of Rosario, Suipacha 570, S2002RLK, Rosario, Argentina. Electronic address: gpico@fbioyf.unr.edu.ar.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Picó
mesh_terms: D000327:Adsorption; D000464:Alginates / Q000737:chemistry*; D000818:Animals; D004529:Egg White / Q000737:chemistry; D004811:Epichlorohydrin / Q000737:chemistry; D005685:Galactans / Q000737:chemistry*; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D007700:Kinetics; D008351:Mannans / Q000737:chemistry*; D009113:Muramidase / Q000737:chemistry*; D053149:Plant Gums / Q000737:chemistry*
chemical_list: D000464:Alginates; D005685:Galactans; D006603:Hexuronic Acids; D008351:Mannans; D053149:Plant Gums; D004811:Epichlorohydrin; D020723:Glucuronic Acid; C007894:guar gum; D009113:Muramidase
keywords: Adsorption; Alginate; Guar gum; Lysozyme; Polyelectrolytes