title: Reactive oxygen species and Ca2+are involved in cadmium-induced cell killing in yeast cells.
abstract: Cadmium (Cd) is one of the most toxic heavy metals. It is of great environmental concern and its toxicity has been investigated in a variety of cells. In this study, we elucidated the toxic effects of Cd in cells of yeast (Saccharomyces cerevisiae). Our results showed that Cd2+(0.05-5.0 mmol·L-1) significantly inhibited yeast cell growth, and the inhibitory effect was positively correlated with Cd2+concentrations. Cd2+caused loss of yeast cell viability in a concentration- and duration-dependent manner. Intracellular reactive oxygen species (ROS) and Ca2+levels increased in yeast cells after exposure to 5.0 mmol·L-1Cd for 6 h. Cd2+-caused cell viability loss was blocked by antioxidants (0.5 mmol·L-1ascorbic acid or 500 U·mL-1catalase) or Ca2+antagonists (0.5 mmol·L-1ethylene glycol tetraacetic acid or 0.5 mmol·L-1LaCl3). Moreover, a collapse of mitochondrial membrane potential (ΔΨm) was observed in Cd2+-treated yeast cells. These results indicate that Cd-induced yeast cell killing was associated with the elevation of intracellular ROS and Ca2+levels and also the loss of ΔΨm.
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.|School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Wang
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.|School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Yi
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.|School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.|School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Yansha
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.|School of Life Science, Shanxi University, Taiyuan 030006, Shanxi, People's Republic of China.
authors.forename: Huilan
authors.initials: H
authors.lastname: Yi
mesh_terms: D000975:Antioxidants / Q000494:pharmacology; D002104:Cadmium / Q000633:toxicity*; D002374:Catalase / Q000494:pharmacology; D017382:Reactive Oxygen Species / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017382:Reactive Oxygen Species; D002104:Cadmium; D002374:Catalase
keywords: Ca2+; cadmium; cell killing; cytotoxicité; levure; reactive oxygen species; réactifs oxygénés; yeast