title: Effect of Perilla frutescens Extracts on Porcine Jejunal Epithelial Cells.
abstract: Green-leaved Perilla frutescens extracts were investigated on their effect on cell proliferation of the porcine jejunal epithelial cell line, IPEC-J2, as well as on the gene expression of cell cycle or cancer-related genes. Some extracted compounds were, however, susceptible to degradation in cell culture medium, whereas others were found to be stable during the entire experimental time. Control experiments also included the assessment of H2O2generation in cell culture medium caused by oxidation of natural extract compounds, which was proved to be absent at low extract concentrations. A fast and significant inhibition of cell growth at low physiological extract concentrations could be observed. This finding, along with an immediate downregulation of 67 kDa laminin receptor and cyclin D1 expression, can be accounted to the presence of Perilla frutescens extract. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Chair of Urban Water Systems Engineering, Technical University of Munich, Am Coulombwall 3, 85748, Garching, Germany.|Institute of Animal Physiology and Immunology Weihenstephan, Technical University of Munich, Weihenstephaner Berg 3, 85354, Freising, Germany.
authors.forename: Christine M
authors.initials: CM
authors.lastname: Kaufmann
authors.affiliation: Chair of Urban Water Systems Engineering, Technical University of Munich, Am Coulombwall 3, 85748, Garching, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Letzel
authors.affiliation: Chair of Urban Water Systems Engineering, Technical University of Munich, Am Coulombwall 3, 85748, Garching, Germany.
authors.forename: Johanna
authors.initials: J
authors.lastname: Grassmann
authors.affiliation: Institute of Animal Physiology and Immunology Weihenstephan, Technical University of Munich, Weihenstephaner Berg 3, 85354, Freising, Germany.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Pfaffl
mesh_terms: D000818:Animals; D049109:Cell Proliferation; D004847:Epithelial Cells / Q000187:drug effects*; D006801:Humans; D007583:Jejunum / Q000187:drug effects*; D010084:Oxidation-Reduction; D031344:Perilla frutescens / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D013552:Swine
chemical_list: D010936:Plant Extracts
keywords: 67 kDa laminin receptor; Perilla frutescens; RT-qPCR; cell proliferation; natural compound stability; reactive oxygen species