title: Resveratrol Protects Sepsis-Induced Oxidative DNA Damage in Liver and Kidney of Rats.
abstract: BACKGROUND
  The increases of free radicals have been proposed to be involved in the pathogenesis of sepsis, which leads to multiple-organ dysfunction syndromes. The uses of antioxidants as a complementary tool in the medical care of oxidative stress-related diseases have attracted attention of researchers. Resveratrol (RV) has suggested being antioxidant, anti-proliferative, and anti-inflammatory effects in various experimental models and clinical settings.
  
  
  OBJECTIVE
  This study was undertaken to evaluate the protective effects of RV on oxidative DNA damage induced by sepsis in the liver and kidney tissues of Wistar albino rats.
  
  
  METHODS
  Animal experimentation.
  
  
  METHODS
  Four experimental groups consisting of eight animals for each was created using a total of thirty-two male Wistar albino rats. Sham group was given 0.5 mL of saline intra-peritoneal (ip) only following laparatomy. Sepsis group was given 0.5 mL saline ip only following the induction of sepsis. RV-treated group was given a dose of 100 mg/kg ip RV in 0.5 mL saline following laparatomy. RV-treated sepsis group was given 100 mg/kg ip RV in 0.5 mL saline following the induction of sepsis. A model of sepsis was created by cecal ligation and puncture technique. In the liver and kidney tissues, oxidative stress parameters (malondialdehyde (MDA), reduced glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GPX)) and a proinflammatory cytokine (tumor necrosis factor alpha (TNF-alpha)), were evaluated spectrophotometrically and DNA damage was determined by the alkaline single cell gel electrophoresis (comet assay) technique using formamidopyrimidine DNA glycosylase protein.
  
  
  RESULTS
  In the RV-treated sepsis group, the levels of MDA and TNF-alpha were lower and GSH levels, SOD and GPX activities were higher than in the septic rats (p<0.05). RV treatment significantly reduced the sepsis-induced oxidative DNA damage in the liver and kidney cells (p<0.05).
  
  
  CONCLUSIONS
  It is suggested that RV treatment might reduce the sepsis-induced oxidative DNA damages in sepsis-related diseases; however, there is a need for more studies to clear up the protective mechanisms of RV against sepsis.
authors.affiliation: Department of Pharmaceutical Toxicology, Hacettepe University School of Pharmacy, Ankara, Turkey.
authors.forename: Sevtap
authors.initials: S
authors.lastname: Aydın
authors.affiliation: Department of Surgery, Kastamonu University School of Medicine, Kastamonu, Turkey.
authors.forename: Tevfik Tolga
authors.initials: TT
authors.lastname: Şahin
authors.affiliation: Department of Pharmaceutical Toxicology, Hacettepe University School of Pharmacy, Ankara, Turkey.
authors.forename: Merve
authors.initials: M
authors.lastname: Bacanlı
authors.affiliation: Department of Bioengineering, Bursa Technical University School of Natural Sciences, Architecture and Engineering, Bursa, Turkey.
authors.forename: Gökçe
authors.initials: G
authors.lastname: Taner
authors.affiliation: Department of Pharmacognosy, Hacettepe University School of Pharmacy, Ankara, Turkey.
authors.forename: Arif Ahmet
authors.initials: AA
authors.lastname: Başaran
authors.affiliation: Department of Infectious Diseases and Clinical Microbiology, Başkent University School of Medicine, İstanbul Hospital, İstanbul, Turkey.
authors.forename: Mehtap
authors.initials: M
authors.lastname: Aydın
authors.affiliation: Department of Pharmaceutical Toxicology, Hacettepe University School of Pharmacy, Ankara, Turkey.
authors.forename: Nurşen
authors.initials: N
authors.lastname: Başaran
keywords: DNA damage; Resveratrol; alkaline single cell gel electrophoresis; oxidative stress; sepsis; tumor necrosis factor alpha