title: Monocarboxylate Transporter-1 Mediates the Protective Effects of Neutral-pH Bicarbonate/Lactate-Buffered Peritoneal Dialysis Fluid on Cell Viability and Apoptosis.
abstract: We investigated the effects of bicarbonate/lactate-buffered peritoneal dialysis fluid (B/L-PDF) and lactate-buffered PDF (L-PDF) on cell viability and apoptosis, focusing on monocarboxylate transporters (MCTs). MCT-1 transports lactate into cells. Cell viability and apoptosis of human peritoneal mesothelial cells (HPMCs) were examined by water-soluble tetrazolium salt-1 and TUNEL assays, respectively. The relative number of viable HPMCs was significantly decreased by L-PDF at 48 h (8.8 ± 0.4%) compared with cells cultured in M199, but not by B/L-PDF (66.7 ± 1.1%). Apoptosis was markedly induced by L-PDF at 48 h (69.3 ± 16.2%), but not by B/L-PDF (2.6 ± 0.3%). Knockdown of MCT-1 by small interfering RNA (siRNA) attenuated the L-PDF-induced reduction of viable cells and increased apoptosis compared with control siRNA, but MCT-4 knockdown had no effect. B/L-PDF had lesser effects on cell viability and apoptosis of HPMCs compared with L-PDF. These results suggest that B/L-PDF biocompatibility occurs by avoiding the induction of apoptosis in HPMCs.
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Akihiro
authors.initials: A
authors.lastname: Kuma
authors.affiliation: Kidney Center, University Hospital of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Tamura
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Nana
authors.initials: N
authors.lastname: Ishimatsu
authors.affiliation: Department of Pathology and Cell Biology, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Yoshikazu
authors.initials: Y
authors.lastname: Harada
authors.affiliation: Department of Occupational Pneumology, Institute of Industrial Ecological Science, University of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Hiroto
authors.initials: H
authors.lastname: Izumi
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Tetsu
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: Kidney Center, University Hospital of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Yumi
authors.initials: Y
authors.lastname: Furuno
authors.affiliation: Kidney Center, University Hospital of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Nakano
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Ryota
authors.initials: R
authors.lastname: Serino
authors.affiliation: Second Department of Internal Medicine, School of Medicine, University of Occupational and Environmental Health, Fukuoka, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Otsuji
mesh_terms: D017209:Apoptosis; D001639:Bicarbonates / Q000378:metabolism*; D002470:Cell Survival; D002478:Cells, Cultured; D015314:Dialysis Solutions / Q000378:metabolism*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D019344:Lactic Acid / Q000378:metabolism*; D027501:Monocarboxylic Acid Transporters / Q000235:genetics / Q000502:physiology*; D010530:Peritoneal Dialysis; D027981:Symporters / Q000235:genetics / Q000502:physiology*; D000099233:Monocarboxylate Transport Protein 1
chemical_list: D001639:Bicarbonates; D015314:Dialysis Solutions; D019344:Lactic Acid; D027501:Monocarboxylic Acid Transporters; D027981:Symporters; D000099233:Monocarboxylate Transport Protein 1
keywords: Apoptosis; Bicarbonate; Biocompatibility; Lactate; Peritoneal dialysis fluid