title: Folic acid-modified soy protein nanoparticles for enhanced targeting and inhibitory.
abstract: Soy protein isolate (SPI) was hydrolyzed by compound enzymes to give water soluble low molecular soy protein (SP). SP and folic acid (FA) modified SP was polymerized with N-3- acrylamidophenylboronic acid (APBA) monomer in aqueous solution to give SP nanoparticles (SP NPs) and FA modified nanoparticles (FA-SP NPs), respectively. These NPs display excellent stability in different conditions, and have a uniform spherical shape with a diameter around of 200nm. Doxorubicin (DOX) was then successfully loaded into SP and FA-SP NPs with a desirable loading content of 13.33% and 16.01%, respectively. The cellular uptake and cytotoxicity of DOX-loaded SP NPs and FA-SP NPs were investigated using the two-dimensional (2D) monolayer cell model and three-dimensional (3D) multicellular spheroids (MCs). In vivo, tumor accumulation and growth inhibitory were then examined using H22 tumor-bearing mice. All these results demonstrated that conjugation of FA can efficiently enhance SP-based NPs' tumor accumulation and antitumor effect.
authors.affiliation: Engineering Research Center for Biomedical Materials, School of Life Science, Anhui University, Hefei 230601, PR China.
authors.forename: Xu
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Engineering Research Center for Biomedical Materials, School of Life Science, Anhui University, Hefei 230601, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Engineering Research Center for Biomedical Materials, School of Life Science, Anhui University, Hefei 230601, PR China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Engineering Research Center for Biomedical Materials, School of Life Science, Anhui University, Hefei 230601, PR China.
authors.forename: Weijing
authors.initials: W
authors.lastname: Yao
authors.affiliation: Engineering Research Center for Biomedical Materials, School of Life Science, Anhui University, Hefei 230601, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Engineering Research Center for Biomedical Materials, School of Life Science, Anhui University, Hefei 230601, PR China. Electronic address: tangrp99@iccas.ac.cn.
authors.forename: Rupei
authors.initials: R
authors.lastname: Tang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D004317:Doxorubicin / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D005492:Folic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D006801:Humans; D008297:Male; D051379:Mice; D008813:Mice, Inbred ICR; D053758:Nanoparticles / Q000737:chemistry*; D009374:Neoplasms, Experimental / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D030262:Soybean Proteins / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D023041:Xenograft Model Antitumor Assays
chemical_list: D030262:Soybean Proteins; D004317:Doxorubicin; D005492:Folic Acid
keywords: Antitumor; Drug delivery; Folic acid; Nanoparticles; Soy protein