title: Microbiological quality of honey from the Pampas Region (Argentina) throughout the extraction process.
abstract: The microbiological quality of honey obtained from different processing points and the environmental quality within honey houses were assessed in the Pampas Region (Argentina). Mold and yeast (MY), culturable heterotrophic mesophilic bacteria (CHMB), the number of spore-forming bacteria as well as the presence of Shigella spp., Salmonella spp. and fecal coliforms were evaluated in 163 samples. These samples were taken from eight honey houses. Results showed that 89 samples had ≤10CFU of MY/g honey, 69 ranged from 10 to 50CFU/g and two reached 65.5CFU/g. Eighty one percent of the samples showed ≤30CFU of CHMB/g honey and only seven samples had between 50 and 54.25CFU/g. Thirty six honey samples were obtained from drums: in 25 samples (69.4%) CHMB counts were less than ≤30CFU/g of honey; in 20 samples (55.5%) the values of MY were between 10 and 50CFU/g honey and total coliforms were only detected in 20 samples. Fecal coliforms, spores of clostridia as well as Salmonella spp. and Shigella spp were not detected and less than 50 spores of Bacillus spp. per g were observed in the honey from drums. Therefore, the microbiological honey quality within the honey houses did not show any sanitary risks. Our results were reported to honey house owners to help them understand the need to reinforce proper honey handling and sanitation practices.
authors.affiliation: Laboratorio de Estudios Apícolas (LabEA-CIC), Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Provincia de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET), Buenos Aires, Argentina.
authors.forename: Leticia A
authors.initials: LA
authors.lastname: Fernández
authors.affiliation: Laboratorio de Estudios Apícolas (LabEA-CIC), Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Provincia de Buenos Aires, Argentina; Laboratorio de Ecología, Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Provincia de Buenos Aires, Argentina.
authors.forename: Carolina
authors.initials: C
authors.lastname: Ghilardi
authors.affiliation: Laboratorio de Estudios Apícolas (LabEA-CIC), Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Provincia de Buenos Aires, Argentina.
authors.forename: Betiana
authors.initials: B
authors.lastname: Hoffmann
authors.affiliation: Laboratorio de Estudios Apícolas (LabEA-CIC), Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Provincia de Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Técnicas de la República Argentina (CONICET), Buenos Aires, Argentina.
authors.forename: Carlos
authors.initials: C
authors.lastname: Busso
authors.affiliation: Laboratorio de Estudios Apícolas (LabEA-CIC), Departamento de Agronomía, Universidad Nacional del Sur, San Andrés 800, 8000 Bahía Blanca, Provincia de Buenos Aires, Argentina. Electronic address: labea@uns.edu.ar.
authors.forename: Liliana M
authors.initials: LM
authors.lastname: Gallez
mesh_terms: D001118:Argentina; D001407:Bacillus / Q000302:isolation & purification; D001419:Bacteria; D005516:Food Microbiology; D005658:Fungi / Q000302:isolation & purification; D006722:Honey / Q000382:microbiology
keywords: Calidad microbiológica; Honey; Honey house; Microbiological quality; Miel; Sala de extracción