title: Bioresponsive polymer coated drug nanorods for breast cancer treatment.
abstract: Ineffective drug release at the target site is among the top challenges for cancer treatment. This reflects the facts that interaction with the physiological condition can denature active ingredients of drugs, and low delivery to the disease microenvironment leads to poor therapeutic outcomes. We hypothesize that depositing a thin layer of bioresponsive polymer on the surface of drug nanoparticles would not only protect drugs from degradation but also allow the release of drugs at the target site. Here, we report a one-step process to prepare bioresponsive polymer coated drug nanorods (NRs) from liquid precursors using the solvent diffusion method. A thin layer (10.3 ± 1.4 nm) of poly(ε-caprolactone) (PCL) polymer coating was deposited on the surface of camptothecin (CPT) anti-cancer drug NRs. The mean size of PCL-coated CPT NRs was 500.9 ± 91.3 nm length × 122.7 ± 10.1 nm width. The PCL polymer coating was biodegradable at acidic pH 6 as determined by Fourier transform infrared spectroscopy. CPT drugs were released up to 51.5% when PCL coating dissolved into non-toxic carboxyl and hydroxyl groups. Trastuzumab (TTZ), a humanized IgG monoclonal antibody, was conjugated to the NR surface for breast cancer cell targeting. Combination treatments using CPT and TTZ decreased the HER-2 positive BT-474 breast cancer cell growth by 66.9 ± 5.3% in vitro. These results suggest effective combination treatments of breast cancer cells using bioresponsive polymer coated drug delivery.
authors.affiliation: Department of Chemical and Biochemical Engineering, Missouri University of Science and Technology, Rolla, MO 65409, USA.
authors.forename: Tunyaboon
authors.initials: T
authors.lastname: Laemthong
authors.forename: Hannah H
authors.initials: HH
authors.lastname: Kim
authors.forename: Kelly
authors.initials: K
authors.lastname: Dunlap
authors.forename: Caitlin
authors.initials: C
authors.lastname: Brocker
authors.forename: Dipak
authors.initials: D
authors.lastname: Barua
authors.forename: Daniel
authors.initials: D
authors.lastname: Forciniti
authors.forename: Yue-Wern
authors.initials: YW
authors.lastname: Huang
authors.forename: Sutapa
authors.initials: S
authors.lastname: Barua
mesh_terms: D001943:Breast Neoplasms / Q000188:drug therapy*; D002166:Camptothecin / Q000494:pharmacology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D065546:Drug Liberation; D005260:Female; D006801:Humans; D007091:Image Processing, Computer-Assisted; D043942:Nanotubes / Q000737:chemistry* / Q000648:ultrastructure; D011091:Polyesters / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D000068878:Trastuzumab / Q000494:pharmacology / Q000627:therapeutic use
chemical_list: D020099:Coated Materials, Biocompatible; D011091:Polyesters; D011108:Polymers; C016240:polycaprolactone; D000068878:Trastuzumab; D002166:Camptothecin