title: Transient receptor potential vanilloid 1-immunoreactive signals in murine enteric glial cells.
abstract: OBJECTIVE
  To investigate the possible involvement of transient receptor potential vanilloid 1 (TRPV1) in maturation of enteric glial cells (EGCs).
  
  
  METHODS
  Immunohistochemical and immunocytochemical techniques were used to analyze EGC markers in myenteric plexus (MP) as well as cultured MP cells and EGCs using TRPV1 knockout (KO) mice.
  
  
  RESULTS
  We detected TRPV1-immunoreactive signals in EGC in the MP of wild-type (WT) but not KO mice. Expression of glial fibrillary acidic protein (GFAP) immunoreactive signals was lower at postnatal day (PD) 6 in KO mice, though the difference was not clear at PD 13 and PD 21. When MP cells were isolated and cultured from isolated longitudinal muscle-MP preparation from WT and KO mice, the yield of KO EGC was lower than that of WT EGC, while the yield of KO and WT smooth muscle cells showed no difference. Addition of BCTC, a TRPV1 antagonist, to enriched EGC culture resulted in a decrease in the protein ratio of GFAP to S100B, another EGC/astrocyte-specific marker.
  
  
  CONCLUSIONS
  These results address the possibility that TRPV1 may be involved in the maturation of EGC, though further studies are necessary to validate this possibility.
authors.affiliation: Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Yamamoto
authors.affiliation: Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors.forename: Mitsue
authors.initials: M
authors.lastname: Nishiyama
authors.affiliation: Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Iizuka
authors.affiliation: Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors.forename: Shigeaki
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors.forename: Norihiro
authors.initials: N
authors.lastname: Suzuki
authors.affiliation: Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors.forename: Sadakazu
authors.initials: S
authors.lastname: Aiso
authors.affiliation: Masahiro Yamamoto, Mitsue Nishiyama, Seiichi Iizuka, Tsumura Research Laboratories, Tsumura & Co., Ibaraki 300-1192, Japan.
authors.forename: Jin
authors.initials: J
authors.lastname: Nakahara
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002478:Cells, Cultured; D018920:Coculture Techniques; D017615:Enteric Nervous System / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D032389:Myocytes, Smooth Muscle / Q000378:metabolism; D009457:Neuroglia / Q000187:drug effects / Q000378:metabolism*; D011719:Pyrazines / Q000494:pharmacology; D011725:Pyridines / Q000494:pharmacology; D064568:S100 Calcium Binding Protein beta Subunit / Q000378:metabolism; D050916:TRPV Cation Channels / Q000037:antagonists & inhibitors / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein; C475772:N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carboxamide; D011719:Pyrazines; D011725:Pyridines; D064568:S100 Calcium Binding Protein beta Subunit; C579159:S100b protein, mouse; D050916:TRPV Cation Channels; C493921:TRPV1 protein, mouse
keywords: Enteric glial cells; Enteric nervous system; Glial fibrillary acidic protein; S100B; Smooth muscle cells