title: Simultaneous quantitation of cytokeratin-19 fragment and carcinoembryonic antigen in human serum via quantum dot-doped nanoparticles.
abstract: A novel quantum dot-doped polystyrene nanoparticles-based lateral flow test strips (QPs-LFTS) system was developed to simultaneously detect a cytokeratin-19 fragment (CYFRA 21-1) and carcinoembryonic antigen (CEA) in human serum to aid the diagnosis and prognosis of lung cancer. Quantum dot-doped carboxylate-functionalized polystyrene nanoparticles (QPs) were prepared and introduced as fluorescent reporters in QPs-LFTS. The detection was based on a sandwich immunoassay and performed on lateral flow test strips, with an assay time of 15min. The strips were read by a fluorescence strip reader to obtain the fluorescence peak heights of the test lines (HT) and the control line (HC). The ratio of HT/HCwas used for quantitation. The QPs showed excellent photoproperties and good performance. Under optimal conditions, the QPs-LFTS system exhibited a wide linear range for CYFRA 21-1 (1.3-480ng/mL) and CEA (2.8-680ng/mL). The detection limits for CYFRA 21-1 and CEA were 0.16 and 0.35ng/mL, respectively. The recovery and reproducibility of the method were satisfactory. Furthermore, excellent correlations (n =120, R2=0.9862, P<0.0001 for CYFRA 21-1; n =70, R2=0.9509, P<0.0001 for CEA) were obtained between the QPs-LFTS and commercially available chemiluminescence immunoassay kits in clinical serum testing. The results indicate that this developed test system is highly efficient and is expected to be useful for early screening and prognosis evaluation for lung cancer patients.
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China.
authors.forename: Rongliang
authors.initials: R
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China.
authors.forename: Junyu
authors.initials: J
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China.
authors.forename: Qiaoting
authors.initials: Q
authors.lastname: Deng
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China.
authors.forename: Taixue
authors.initials: T
authors.lastname: An
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China; Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China. Electronic address: liutc@smu.edu.cn.
authors.forename: Tiancai
authors.initials: T
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Organ Failure Research, Institute of Antibody Engineering, School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou 510515, Guangdong, People's Republic of China. Electronic address: wg@smu.edu.cn.
authors.forename: Yingsong
authors.initials: Y
authors.lastname: Wu
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D000951:Antigens, Neoplasm / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D019187:Cadmium Compounds / Q000737:chemistry; D002272:Carcinoembryonic Antigen / Q000097:blood*; D004867:Equipment Design; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D053539:Keratin-19 / Q000097:blood*; D057230:Limit of Detection; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis; D053758:Nanoparticles / Q000737:chemistry / Q000648:ultrastructure; D011137:Polystyrenes / Q000737:chemistry*; D011379:Prognosis; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D011934:Reagent Strips / Q000032:analysis*; D015203:Reproducibility of Results; D013440:Sulfides / Q000737:chemistry; D013691:Tellurium / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D019187:Cadmium Compounds; D002272:Carcinoembryonic Antigen; D005456:Fluorescent Dyes; D053539:Keratin-19; D011137:Polystyrenes; D011934:Reagent Strips; D013440:Sulfides; C102012:antigen CYFRA21.1; C034939:cadmium sulfide; D013691:Tellurium; C028337:cadmium telluride
keywords: CEA; CYFRA 21-1; Lateral flow test strips; Polystyrene nanoparticle; Quantum dots; Simultaneous quantitation