title: Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging.
abstract: Ganoderma lucidum has featured in traditional Chinese medicine for >1,000 years. Ganoderma polysaccharides (GL-PS), a major active ingredient in Ganoderma, confer immune regulation, antitumor effects and significant antioxidant effects. The aim of the present study was to investigate the efficacy and mechanism of GL‑PS‑associated inhibition of ultraviolet B (UVB)‑induced photoaging in human fibroblasts in vitro. Primary human skin fibroblasts were cultured, and a fibroblast photoaging model was built through exposure to UVB. Cell viability was measured by MTT assay. Aged cells were stained using a senescence‑associated β-galactosidase staining (SA‑β‑gal) kit. ELISA kits were used to analyze matrix metalloproteinase (MMP) ‑1 and C‑telopeptides of Type I collagen (CICP) protein levels in cellular supernatant. ROS levels were quantified by flow cytometry. Cells exposed to UVB had decreased cell viability, increased aged cells, decreased CICP protein expression, increased MMP‑1 protein expression, and increased cellular ROS levels compared with non‑exposed cells. However, cells exposed to UVB and treated with 10, 20 and 40 µg/ml GL‑PS demonstrated increased cell viability, decreased aged cells, increased CICP protein expression, decreased MMP‑1 protein expression, and decreased cellular ROS levels compared with UVB exposed/GL‑PS untreated cells. These results demonstrate that GL‑PS protects fibroblasts against photoaging by eliminating UVB‑induced ROS. This finding indicates GL‑PS treatment may serve as a novel strategy for antiphotoaging.
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Qinghai
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Lei
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of Burn and Plastic Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jianda
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Oncology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Ke
authors.initials: K
authors.lastname: Cao
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Oncology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Xia
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Shu
authors.initials: S
authors.lastname: Ding
authors.affiliation: The Central Laboratory, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Huang
authors.affiliation: The Central Laboratory, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xiang
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Yangfan
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xiao
authors.affiliation: Department of Dermatology, Third Xiangya Hospital, Central South University, Changsha, Hunan 410013, P.R. China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Huang
mesh_terms: D000293:Adolescent; D002470:Cell Survival / Q000187:drug effects / Q000528:radiation effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects* / Q000528:radiation effects*; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism / Q000528:radiation effects*; D006801:Humans; D008297:Male; D020781:Matrix Metalloproteinase 1 / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D011837:Radiation-Protective Agents / Q000737:chemistry / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D020940:Reishi / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D011134:Polysaccharides; D011837:Radiation-Protective Agents; D017382:Reactive Oxygen Species; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1