title: Engineered Kluyveromyces marxianus for pyruvate production at elevated temperature with simultaneous consumption of xylose and glucose.
abstract: Xylose and glucose from lignocellulose are sustainable sources for production of pyruvate, which is the starting material for the synthesis of many drugs and agrochemicals. In this study, the pyruvate decarboxylase gene (KmPDC1) and glycerol-3-phosphate dehydrogenase gene (KmGPD1) of Kluyveromyces marxianus YZJ051 were disrupted to prevent ethanol and glycerol accumulation. The deficient growth of PDC disruption was rescued by overexpressing mutant KmMTH1-ΔT. Then pentose phosphate pathway and xylitol dehydrogenase SsXYL2-ARS genes were overexpressed to obtain strain YZB053 which produced pyruvate with xylose other than glucose. It produced 24.62g/L pyruvate from 80g/L xylose with productivity of 0.51g/L/h at 42°C. Then, xylose-specific transporter ScGAL2-N376F was overexpressed to obtain strain YZB058, which simultaneously consumed 40g/L glucose and 20g/L xylose and produced 29.21g/L pyruvate with productivity of 0.81g/L/h at 42°C. Therefore, a platform for pyruvate production from glucose and xylose at elevated temperature was developed.
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
authors.forename: Biao
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
authors.forename: Yelin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Single-Cell Center, CAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China.
authors.forename: Jia
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Wang
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, PR China.
authors.forename: Lianhong
authors.initials: L
authors.lastname: Sun
authors.affiliation: School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, PR China. Electronic address: hjiong@ustc.edu.cn.
authors.forename: Jiong
authors.initials: J
authors.lastname: Hong
mesh_terms: D050542:D-Xylulose Reductase / Q000235:genetics / Q000378:metabolism; D000431:Ethanol / Q000378:metabolism; D005285:Fermentation; D015966:Gene Expression Regulation, Fungal; D005818:Genetic Engineering / Q000379:methods*; D005947:Glucose / Q000235:genetics / Q000378:metabolism*; D005990:Glycerol / Q000378:metabolism; D005993:Glycerolphosphate Dehydrogenase / Q000235:genetics / Q000378:metabolism; D007716:Kluyveromyces / Q000235:genetics / Q000378:metabolism*; D011767:Pyruvate Decarboxylase / Q000235:genetics / Q000378:metabolism; D019289:Pyruvic Acid / Q000378:metabolism*; D013696:Temperature; D014994:Xylose / Q000235:genetics / Q000378:metabolism*
chemical_list: D000431:Ethanol; D019289:Pyruvic Acid; D014994:Xylose; D005993:Glycerolphosphate Dehydrogenase; D050542:D-Xylulose Reductase; D011767:Pyruvate Decarboxylase; D005947:Glucose; D005990:Glycerol
keywords: Cofermentation; Elevated temperature; Kluyveromyces marxianus; Pyruvate; Xylose