title: Heterologous expression of Paranosema (Antonospora) locustae hexokinase in lepidopteran, Sf9, cells is followed by accumulation of the microsporidian protein in insect cell nuclei.
abstract: Paranosema (Nosema, Antonospora) locustae is the only microsporidium produced as a commercial product for biological control. Molecular mechanisms of the effects of this pathogen and other invertebrate microsporidia on host cells remain uncharacterized. Previously, we immunolocalized P. locustae hexokinase in nuclei of Locusta migratoria infected adipocytes. Here, the microsporidian protein was expressed in the yeast Pichia pastoris and in lepidopteran Sf9 cells. During heterologous expression, P. locustae hexokinase was accumulated in the nuclei of insect cells but not in yeast cell nuclei. This confirms nuclear localization of hexokinase secreted by microsporidia into infected host cells and suggests convenient model for its further study.
authors.affiliation: Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Timofeev
authors.affiliation: Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia.
authors.forename: Igor V
authors.initials: IV
authors.lastname: Senderskiy
authors.affiliation: Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Tsarev
authors.affiliation: Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia.
authors.forename: Yuri S
authors.initials: YS
authors.lastname: Tokarev
authors.affiliation: Laboratory of Microbiological Control, All-Russian Institute for Plant Protection, St. Petersburg, Pushkin, Russia. Electronic address: dol1slav@yahoo.com.
authors.forename: Viacheslav V
authors.initials: VV
authors.lastname: Dolgikh
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism; D005656:Fungal Proteins / Q000096:biosynthesis*; D006593:Hexokinase / Q000096:biosynthesis*; D016881:Microsporidiosis / Q000662:veterinary; D016818:Nosema / Q000201:enzymology*; D010843:Pichia; D018411:Spodoptera / Q000469:parasitology*
chemical_list: D005656:Fungal Proteins; D006593:Hexokinase
keywords: Heterologous expression; Hexokinase; Insect pathogens; Intracellular parasites; Microsporidia; Nuclear localization; Paranosema locustae; Secretory proteins