title: Electrospun biocompatible Chitosan/MIL-101 (Fe) composite nanofibers for solid-phase extraction ofΔ9-tetrahydrocannabinol in whole blood samples using Box-Behnken experimental design.
abstract: The nanofibers of biocompatible Chitosan/MIL-101 (Fe) composite were synthesized by a simple, cheap and accessible electrospining method and applied for mat-based extraction of trace amount of Δ9-tetrahydrocannabinol (THC) from human whole blood sample following its combination by high performance liquid chromatography-ultraviolet detection. The composite nanofibres were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, X-ray diffraction and N2adsorption-desorption experiments. The volume of eluting solvent, sorbent amount, pH and% NaCl (w/v) influencing on the responses were investigated using factorial experimental design. The optimum point was achieved by analysis of the results according to design expert (DX) software. The volume of eluting solvent, sorbent amount and pH were significant variables, and 150μL, 7mg and 7.0 were respectively chosen for obtaining the best extraction response. Under the optimum conditions, the method was exhibited a linear range of 0.1-100μgL-1(R2=0.9943) for THC with a detection limit of 0.04μgL-1. Acceptable values for intra-day (3.2%) and inter-day (4.8%) relative standard deviations were obtained. The high preconcentration factor (970) and satisfactory recoveries (88.2%-92.4%) in whole blood samples were achieved which proved the capability of the method for trace determination of THC in the human whole blood samples.
authors.affiliation: Department of Chemistry, Faculty of Science, K. N. Toosi University of Technology, Tehran, Iran.
authors.forename: Mina
authors.initials: M
authors.lastname: Asiabi
authors.affiliation: Department of Marine Living Science, Ocean Sciences Research Center, Iranian National Institute for Oceanography and Atmospheric Science, Tehran, Iran. Electronic address: mehdinia@inio.ac.ir.
authors.forename: Ali
authors.initials: A
authors.lastname: Mehdinia
authors.affiliation: Department of Chemistry, Faculty of Science, K. N. Toosi University of Technology, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Jabbari
mesh_terms: D000327:Adsorption; D001774:Blood Chemical Analysis / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D056831:Coordination Complexes / Q000737:chemistry*; D013759:Dronabinol / Q000097:blood*; D006801:Humans; D057230:Limit of Detection; D000073396:Metal-Organic Frameworks; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D012107:Research Design; D052616:Solid Phase Extraction; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D056831:Coordination Complexes; C000589635:MIL-101; D000073396:Metal-Organic Frameworks; D013759:Dronabinol; D048271:Chitosan
keywords: Biocompatible composite nanofibers; Central composite design; Chitosan/MIL-101 (Fe); Electrospining; Δ9-Tetrahydrocannabinol