title: APC/CCDC20and APC/C play pivotal roles in the process of embryonic development in Artemia sinica.
abstract: Anaphase Promoting Complex or Cyclosome (APC/C) is a representative E3 ubiquitin ligase, triggering the transition of metaphase to anaphase by regulating degradation and ensures the exit from mitosis. Cell division cycle 20 (CDC20) and Cell division cycle 20 related protein 1 (CDH1), as co-activators of APC/C, play significant roles in the spindle assembly checkpoint, guiding ubiquitin-mediated degradation, together with CDC23. During the embryonic development of the brine shrimp, Artemia sinica, CDC20, CDH1 and CDC23 participate in cell cycle regulation, but the specific mechanisms of their activities remain unknown. Herein, the full-length cDNAs of cdc20 and cdc23 from A. sinica were cloned. Real-time PCR analyzed the expression levels of As-cdc20 and As-cdc23. The locations of CDH1, CDC20 and CDC23 showed no tissue or organ specificity. Furthermore, western blotting showed that the levels of As-CDC20, securin, cyclin B, CDK1, CDH1, CDC14B, CDC23 and geminin proteins conformed to their complicated degradation relationships during different embryo stages. Our research revealed that As-CDC20, As-CDH1 and APC mediate the mitotic progression, downstream proteins degradation and cellular differentiation in the process of embryonic development in A. sinica.
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Mengchen
authors.initials: M
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yao
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Luan
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Jing
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Liaoning Normal University, Dalian 116081, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Hou
authors.affiliation: Department of Biology, Dalian Medical University, Dalian 116044, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zou
mesh_terms: D064173:Anaphase-Promoting Complex-Cyclosome / Q000378:metabolism*; D000818:Animals; D001156:Artemia / Q000196:embryology*; D060829:Arthropod Proteins / Q000378:metabolism; D064199:Cdc20 Proteins / Q000235:genetics* / Q000378:metabolism*; D002454:Cell Differentiation; D003001:Cloning, Molecular; D047108:Embryonic Development; D018507:Gene Expression Regulation, Developmental; D008938:Mitosis; D059748:Proteolysis
chemical_list: D060829:Arthropod Proteins; D064199:Cdc20 Proteins; D064173:Anaphase-Promoting Complex-Cyclosome