title: Gene silencing of Dim-1, a member of the disorganized muscle family, in Haemonchus contortus.
abstract: RNA interference has been widely used in parasites. In Haemonchus contortus, reproducible silencing has been reported; however, in this species an altered phenotype has been observed for only a few genes silenced by RNA interference. The aim of this work was to evaluate the effect of silencing Dim-1, a member of the disorganized muscle family on third stage larvae (L3) of H. contortus. Effective silencing of Dim-1 in L3 led to reduced L3 migration and slowed larval development from L3 to early L4. To our knowledge, this is the first report to demonstrate Dim-1 silencing affecting phenotypic characteristics of this parasite.
authors.affiliation: Department of Morphophysiology, University of Camagüey, Circunvalación Norte, Km 5 1/2, 74650, Camagüey, Cuba. Electronic address: amilcar.arenal@reduc.edu.cu.
authors.forename: Amilcar
authors.initials: A
authors.lastname: Arenal
authors.affiliation: Department of Morphophysiology, University of Camagüey, Circunvalación Norte, Km 5 1/2, 74650, Camagüey, Cuba.
authors.forename: Arnielis
authors.initials: A
authors.lastname: Díaz
authors.affiliation: Laboratório de Doenças Parasitárias, Universidade Federal do Paraná, Rua dos Funcionários 1540, CEP: 80.035-050, Curitiba, PR, Brazil.
authors.forename: Lew Kan
authors.initials: LK
authors.lastname: Sprenger
authors.affiliation: Laboratório de Doenças Parasitárias, Universidade Federal do Paraná, Rua dos Funcionários 1540, CEP: 80.035-050, Curitiba, PR, Brazil.
authors.forename: Andreia
authors.initials: A
authors.lastname: Buzatti
authors.affiliation: Laboratório de Doenças Parasitárias, Universidade Federal do Paraná, Rua dos Funcionários 1540, CEP: 80.035-050, Curitiba, PR, Brazil.
authors.forename: Maria Angela Machado
authors.initials: MA
authors.lastname: Fernandes
authors.affiliation: Laboratory of Cell Biology and Helminthology "Profa. Dra. Reinalda Marisa Lanfredi," Institute of Biological Sciences, Federal University of Pará (Universidade Federal do Pará, UFPA), Augusto Corrêa, 01-Guamá, Belém 66075-110, PA, Brazil.
authors.forename: Jeannie Nascimento
authors.initials: JN
authors.lastname: Dos Santos
authors.affiliation: Laboratório de Doenças Parasitárias, Universidade Federal do Paraná, Rua dos Funcionários 1540, CEP: 80.035-050, Curitiba, PR, Brazil; National Institute of Science and Technology, INCT - Livestock, Belo Horizonte, Brazil.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Molento
mesh_terms: D000818:Animals; D020868:Gene Silencing; D006188:Haemonchiasis / Q000469:parasitology; D006189:Haemonchus / Q000235:genetics* / Q000254:growth & development; D015801:Helminth Proteins / Q000235:genetics*; D007814:Larva; D034622:RNA Interference; D012756:Sheep
chemical_list: D015801:Helminth Proteins
keywords: Larval development; Larval migration; RNA interference