title: Short-term effects of T-2 toxin or deoxynivalenol on lipid peroxidation and the glutathione system in common carp.
abstract: The purpose of this study was to investigate the short-term effects of a single oral dose of T-2 and HT-2 toxin at 0.15, 0.33 and 1.82 mg kg-1body weight, or deoxynivalenol (DON) and 15-acetyl-DON at 0.13, 0.31 and 1.75 mg kg-1body weight in common carp. Conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all DON-treated groups at the 16th hour, while thiobarbituric acid reactive substances (TBARS; termination marker) were increased at the highest dose of DON at the 16th and 24th hours. T-2 toxin did not cause changes in these parameters. Glutathione content and glutathione peroxidase activity showed higher levels at the 16th hour as the effect of both mycotoxins. The expression of glutathione peroxidase (GPx4) genes (gpx4a and gpx4b) revealed a dual response. Downregulation was observed at the 8th hour, followed by an induction at the 16th hour, at the lowest dose of both mycotoxins. Higher doses revealed long-drawn emergence and an elevation was observed only at the 24th hour. However, at the lowest and highest doses of DON or T-2 toxin the changes in gene expression were delayed, which may be related to the low oxidative stress response, as suggested by the expression profiles of the nrf2, keap1, gpx4a and gpx4b genes.
authors.affiliation: Department of Nutrition, Szent István University , Páter Károly u. 1, H-2103 Gödöllő , Hungary.
authors.forename: Csilla
authors.initials: C
authors.lastname: Pelyhe
authors.affiliation: Department of Nutrition, Szent István University , Páter Károly u. 1, H-2103 Gödöllő , Hungary.
authors.forename: Benjámin
authors.initials: B
authors.lastname: Kövesi
authors.affiliation: Academy of Sciences-Kaposvár University , Mycotoxins in the Food Chain Research Group, Kaposvár , Hungary.
authors.forename: Erika
authors.initials: E
authors.lastname: Zándoki
authors.affiliation: Department of Aquaculture, Szent István University , Páter Károly u. 1, H-2103 Gödöllő , Hungary.
authors.forename: Balázs
authors.initials: B
authors.lastname: Kovács
authors.affiliation: Academy of Sciences-Kaposvár University , Mycotoxins in the Food Chain Research Group, Kaposvár , Hungary.
authors.forename: Judit
authors.initials: J
authors.lastname: Szabó-Fodor
authors.affiliation: Department of Nutrition, Szent István University , Páter Károly u. 1, H-2103 Gödöllő , Hungary.
authors.forename: Miklós
authors.initials: M
authors.lastname: Mézes
authors.affiliation: Department of Nutrition, Szent István University , Páter Károly u. 1, H-2103 Gödöllő , Hungary.
authors.forename: Krisztián
authors.initials: K
authors.lastname: Balogh
mesh_terms: D000821:Animal Feed / Q000032:analysis; D000818:Animals; D002347:Carps / Q000378:metabolism*; D005506:Food Contamination; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism*; D005979:Glutathione Peroxidase / Q000235:genetics / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects*; D013605:T-2 Toxin / Q000633:toxicity*; D014255:Trichothecenes / Q000633:toxicity*
chemical_list: D014255:Trichothecenes; D005979:Glutathione Peroxidase; D005978:Glutathione; D013605:T-2 Toxin; C007262:deoxynivalenol
keywords: Common carp; T-2 toxin; deoxynivalenol; glutathione peroxidase; gpx genes