title: Effects of dilute acid and flowthrough pretreatments and BSA supplementation on enzymatic deconstruction of poplar by cellulase and xylanase.
abstract: To help understand factors controlling the recalcitrance of lignocellulosic biomass to deconstruction to sugars, poplar was pretreated with liquid hot water (LHW) and extremely dilute acid (EDA) at 140°C and 180°C in batch and flowthrough reactors. The resulting solids were then subjected to enzymatic hydrolysis by eight combinations of cellulase, xylanase, and bovine serum albumin (BSA). Co-addition of xylanase to cellulase resulted in up to 11 percentage points higher overall sugar yield than their sequential addition. In general, supplementation of BSA to enzymes had a larger impact on flowthrough solids with reduced lignin content than batch solids with high lignin content. BSA did not affect xylan yields and while it had low impact on LHW solids, it caused large increases in sugar yields from EDA solids. Flowthrough pretreatment produced less recalcitrant solids than did batch operation, but using very dilute acid reduced recalcitrance even more.
authors.affiliation: Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: sbhag001@ucr.edu.
authors.forename: Samarthya
authors.initials: S
authors.lastname: Bhagia
authors.affiliation: Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: rkumar@cert.ucr.edu.
authors.forename: Rajeev
authors.initials: R
authors.lastname: Kumar
authors.affiliation: Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California Riverside, 900 University Ave, Riverside, CA 92521, USA; Center for Environmental Research and Technology, Bourns College of Engineering, University of California Riverside, 1084 Columbia Ave, Riverside, CA 92507, USA; BioEnergy Science Center (BESC), Oak Ridge National Laboratory (ORNL), Oak Ridge, TN 37831, USA. Electronic address: cewyman@engr.ucr.edu.
authors.forename: Charles E
authors.initials: CE
authors.lastname: Wyman
mesh_terms: D002480:Cellulase / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism; D032107:Populus; D012710:Serum Albumin, Bovine; D014934:Wood
chemical_list: D012710:Serum Albumin, Bovine; D002482:Cellulose; D008031:Lignin; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: Bovine serum albumin; Cellulase; Dilute acid; Flowthrough pretreatment; Poplar; Xylanase