title: Skin targeting of resveratrol utilizing solid lipid nanoparticle-engrossed gel for chemically induced irritant contact dermatitis.
abstract: Irritant contact dermatitis (ICD) is a chronic and relapsing skin disease with severe eczematous lesions. Despite its growing prevalence, therapeutic treatments remain limited. Long-term topical corticosteroid treatment can induce skin atrophy, hypopigmentation, and increase in transepidermal water loss. An innovative dermal treatment is essential to reduce the side effects of corticosteroids. Topical resveratrol (RES), although effective for ICD, is a challenging molecule due to low solubility and poor bioavailability. The objective of this work was to build RES-loaded solid lipid nanoparticles (RES-SLNs) with skin targeting. For this purpose, RES-SLNs were prepared using the probe ultrasonication method utilizing Precirol ATO 5 and Tween 20. The RES-SLNs were evaluated for particle size, entrapment efficiency (EE), and transmission electron microscopy (TEM) studies. Further, RES-SLNs were incorporated into Carbopol gel and investigated for ex vivo skin permeation, deposition study on human cadaver skin, and finally skin irritation study on New Zealand White rabbits. It was further assessed for possible beneficial effects on ICD using BALB/c mice. RES-SLN showed mean size below 100 nm and 68-89% EE. TEM studies confirmed spherical particles in the nanometer range. An ex vivo study of RES-SLN-loaded gel exhibited controlled drug release up to 24 h; similarly, in vitro drug deposition studies showed potential of skin targeting with no skin irritation. RES-SLN gel confirmed competent suppression of ear swelling and reduction in skin water content in the BALB/c mouse model of ICD when compared to marketed gel. Thus, the formulated RES-SLN gel would be a safe and effective alternative to conventional vehicles for treatment of ICD.
authors.affiliation: Sinhgad College of Pharmacy, Vadgaon(Bk.), Pune S. No 44/1, Vadgaon (Bk), Off Sinhgad Road, Pune, MS, 411041, India. snshrotriya2014@gmail.com.
authors.forename: S N
authors.initials: SN
authors.lastname: Shrotriya
authors.affiliation: Sinhgad College of Pharmacy, Vadgaon(Bk.), Pune S. No 44/1, Vadgaon (Bk), Off Sinhgad Road, Pune, MS, 411041, India.
authors.forename: N S
authors.initials: NS
authors.lastname: Ranpise
authors.affiliation: Sinhgad College of Pharmacy, Vadgaon(Bk.), Pune S. No 44/1, Vadgaon (Bk), Off Sinhgad Road, Pune, MS, 411041, India.
authors.forename: B V
authors.initials: BV
authors.lastname: Vidhate
mesh_terms: D000818:Animals; D017453:Dermatitis, Irritant / Q000188:drug therapy / Q000209:etiology; D004137:Dinitrochlorobenzene; D004337:Drug Carriers / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D005260:Female; D005782:Gels / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D006801:Humans; D007509:Irritants; D008055:Lipids / Q000737:chemistry; D008297:Male; D008807:Mice, Inbred BALB C; D053758:Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use; D011817:Rabbits; D000077185:Resveratrol; D012212:Rheology; D012867:Skin / Q000378:metabolism; D013267:Stilbenes / Q000008:administration & dosage* / Q000737:chemistry / Q000627:therapeutic use
chemical_list: D004137:Dinitrochlorobenzene; D004337:Drug Carriers; D005782:Gels; D007509:Irritants; D008055:Lipids; D013267:Stilbenes; D000077185:Resveratrol
keywords: Irritant contact dermatitis; Resveratrol; SLN engrossed gel; Skin targeting; Solid lipid nanoparticles