title: Characterization, classification and authentication of fruit-based extracts by means of HPLC-UV chromatographic fingerprints, polyphenolic profiles and chemometric methods.
abstract: HPLC-UV was applied to the analysis and characterization of fruit-based and fruit-processed products. A Kinetex C18 reversed-phase column was proposed under gradient elution for the determination of 17 polyphenols. Acceptable sensitivity (LODs below 0.16mg/L), and good linearity (r2higher than 0.995), precision (RSD below 6.8%), and method trueness (relative errors below 11%) were obtained. Data corresponding to polyphenolic peak areas and HPLC-UV chromatographic fingerprints were then analyzed by exploratory principal component analysis (PCA) to extract information of the most significant variables contributing to characterization and classification of analyzed samples regarding the fruit of origin. HPLC-UV chromatographic data was further treated by partial least square (PLS) regression to determine the percentages of adulteration in cranberry-fruit extracts. It was found that even mixture samples containing low percentages of adulterants could be distinguished from genuine cranberry extracts. Highly satisfactory results were obtained, with overall errors in the quantification of adulterations below 4.3%.
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain.
authors.forename: Naiara
authors.initials: N
authors.lastname: Pardo-Mates
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain.
authors.forename: Alex
authors.initials: A
authors.lastname: Vera
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Barbosa
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain.
authors.forename: Míriam
authors.initials: M
authors.lastname: Hidalgo-Serrano
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain; Research Institute in Food Nutrition and Food Safety, University of Barcelona, Recinte Torribera, Av. Prat de la Riba 171, Edifici de Recerca (Gaudí), E-08921 Santa Coloma de Gramenet, Barcelona, Spain; Serra Húnter Fellow, Generalitat de Catalunya, Spain. Electronic address: oscar.nunez@ub.edu.
authors.forename: Oscar
authors.initials: O
authors.lastname: Núñez
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain; Research Institute in Food Nutrition and Food Safety, University of Barcelona, Recinte Torribera, Av. Prat de la Riba 171, Edifici de Recerca (Gaudí), E-08921 Santa Coloma de Gramenet, Barcelona, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: Saurina
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain; Research Institute in Food Nutrition and Food Safety, University of Barcelona, Recinte Torribera, Av. Prat de la Riba 171, Edifici de Recerca (Gaudí), E-08921 Santa Coloma de Gramenet, Barcelona, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Hernández-Cassou
authors.affiliation: Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Martí i Franquès 1-11, E08028 Barcelona, Spain; Research Institute in Food Nutrition and Food Safety, University of Barcelona, Recinte Torribera, Av. Prat de la Riba 171, Edifici de Recerca (Gaudí), E-08921 Santa Coloma de Gramenet, Barcelona, Spain.
authors.forename: Lluis
authors.initials: L
authors.lastname: Puignou
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D000068598:Data Accuracy; D005638:Fruit / Q000737:chemistry* / Q000145:classification; D010936:Plant Extracts / Q000032:analysis* / Q000145:classification; D059808:Polyphenols / Q000032:analysis*; D025341:Principal Component Analysis; D012680:Sensitivity and Specificity
chemical_list: D010936:Plant Extracts; D059808:Polyphenols
keywords: Food authentication; High performance liquid chromatography; Partial least square regression; Polyphenols; Principal component analysis; UV-detection