title: Fucoidan inhibits LPS-induced inflammation in vitro and during the acute response in vivo.
abstract: Studies have been focused on natural products with antibacterial and anti-inflammatory activities, such as fucoidan. Many in vivo studies have evaluated the effect of fucoidan on tumor growth, diabetes, obesity, ischemia reperfusion, and oxidative stress. However, the effects of fucoidan on bacteria-induced gingival inflammation and periodontitis have not been reported. We previously characterized the anti-inflammatory effect of fucoidan in vitro. Here, we confirmed the anti-inflammatory activity of fucoidan in a macrophage cell line in terms of its inhibition of the expression of inflammatory mediators and pro-inflammatory cytokines. Additionally, we confirmed the ability of fucoidan to inhibit gingival inflammation, expression of pro-inflammatory cytokines, and neutrophil recruitment in the gingival tissue of mice injected with LPS prepared from P. gingivalis. Interestingly, however, fucoidan did not inhibit the expression of pro-inflammatory cytokines in a P. gingivalis-infected mouse model of periodontitis. Additionally, fucoidan treatment did not lead to clearance of P. gingivalis or improvement of P. gingivalis infection-mediated bone loss in the periodontitis model. We conclude that fucoidan exerts anti-inflammatory effects in vitro and in vivo, together with a limited antibacterial effect in vivo.
authors.affiliation: Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 54896, Republic of Korea.
authors.forename: Jisang
authors.initials: J
authors.lastname: Park
authors.affiliation: Department of Oral Microbiology and Institute of Oral Bioscience, Chonbuk National University, Jeonju 54896, Republic of Korea; Department of Efficacy Research, Institute of Jinan Red Ginseng, Jinan 55442, Republic of Korea.
authors.forename: Jeong-Dan
authors.initials: JD
authors.lastname: Cha
authors.affiliation: Department of Efficacy Research, Institute of Jinan Red Ginseng, Jinan 55442, Republic of Korea.
authors.forename: Kyung-Min
authors.initials: KM
authors.lastname: Choi
authors.affiliation: Department of Oral Microbiology and Institute of Oral Bioscience, Chonbuk National University, Jeonju 54896, Republic of Korea.
authors.forename: Kyung-Yeol
authors.initials: KY
authors.lastname: Lee
authors.affiliation: Department of Pathology, Dongguk University Ilsan Hospital, Goyang 10326, Republic of Korea. Electronic address: kiekie53@hanmail.net.
authors.forename: Kang Min
authors.initials: KM
authors.lastname: Han
authors.affiliation: Department of Bioactive Material Sciences and Research Center of Bioactive Materials, Chonbuk National University, Jeonju 54896, Republic of Korea; Department of Molecular Biology and the Institute for Molecular Biology and Genetics, Chonbuk National University, Jeonju 54896, Republic of Korea. Electronic address: yongsuk@jbnu.ac.kr.
authors.forename: Yong-Suk
authors.initials: YS
authors.lastname: Jang
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000627:therapeutic use*; D016866:Bacteroidaceae Infections / Q000188:drug therapy*; D002465:Cell Movement / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D004195:Disease Models, Animal; D005881:Gingiva / Q000187:drug effects* / Q000276:immunology / Q000382:microbiology; D005891:Gingivitis / Q000139:chemically induced / Q000188:drug therapy*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism; D008070:Lipopolysaccharides / Q000276:immunology; D051379:Mice; D008815:Mice, Inbred Strains; D009504:Neutrophils / Q000187:drug effects* / Q000276:immunology; D010518:Periodontitis / Q000188:drug therapy*; D011134:Polysaccharides / Q000627:therapeutic use*; D016966:Porphyromonas gingivalis / Q000276:immunology*; D000067996:RAW 264.7 Cells
chemical_list: D000893:Anti-Inflammatory Agents; D016207:Cytokines; D018836:Inflammation Mediators; D008070:Lipopolysaccharides; D011134:Polysaccharides; C007789:fucoidan
keywords: Anti-inflammatory activity; Antibacterial activity; Fucoidan; P. gingivalis; Periodontitis