title: De novotranscriptome assembly of the mycoheterotrophic plantMonotropa hypopitys.
abstract: Monotropa hypopitys(pinesap) is a non-photosynthetic obligately mycoheterotrophic plant of the familyEricaceae. It obtains the carbon and other nutrients from the roots of surrounding autotrophic trees through the associated mycorrhizal fungi. In order to understand the evolutionary changes in the plant genome associated with transition to a heterotrophic lifestyle, we performedde novotranscriptomic analysis ofM. hypopitysusing next-generation sequencing. We obtained the RNA-Seq data from flowers, flower bracts and roots with haustoria using Illumina HiSeq2500 platform. The raw data obtained in this study can be available in NCBI SRA database with accession number of SRP069226. A total of 10.3 GB raw sequence data were obtained, corresponding to 103,357,809 raw reads. A total of 103,025,683 reads were filtered after removing low-quality reads and trimming the adapter sequences. The Trinity program was used tode novoassemble 98,349 unigens with an N50 of 1342 bp. Using the TransDecoder program, we predicted 43,505 putative proteins. 38,416 unigenes were annotated in the Swiss-Prot protein sequence database using BLASTX. The obtained transcriptomic data will be useful for further studies of the evolution of plant genomes upon transition to a non-photosynthetic lifestyle and the loss of photosynthesis-related functions.
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Alexey V
authors.initials: AV
authors.lastname: Beletsky
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Mikhail A
authors.initials: MA
authors.lastname: Filyushin
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Eugeny V
authors.initials: EV
authors.lastname: Gruzdev
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Alexander M
authors.initials: AM
authors.lastname: Mazur
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Egor B
authors.initials: EB
authors.lastname: Prokhortchouk
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Elena Z
authors.initials: EZ
authors.lastname: Kochieva
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Andrey V
authors.initials: AV
authors.lastname: Mardanov
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Nikolai V
authors.initials: NV
authors.lastname: Ravin
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
authors.forename: Konstantin G
authors.initials: KG
authors.lastname: Skryabin
keywords: Monotropa hypopitys; Parasitic plant; RNA-Seq; Transcriptome