title: The peroxidase and oxidase-like activity of NiCo2O4mesoporous spheres: Mechanistic understanding and colorimetric biosensing.
abstract: The synthesized NiCo2O4mesoporous spheres (MS) displayed intrinsic peroxidase and oxidase-like activity were firstly reported. The catalytic mechanism of the oxidase-like activity of NiCo2O4MS was analyzed in detail using the electron spin resonance (ESR) method. It is found that NiCo2O4MS could directly oxidize 3,3',5,5'-tetramethylbenzidine (TMB) but did not produce1O2and ·OH. And the mechanism of the peroxidase-like activity of NiCo2O4MS was also verified that the oxidation of TMB stemmed from not only ·OH but also1O2. Based on the NiCo2O4MS showed excellent peroxidase-like activity over a broad temperature range, especially at normal body temperature, a detection tool was designed for glucose determination in diabetics' serum samples. And this detection method based on NiCo2O4MS gave a lower limit of detection than the method using Co3O4NPs and NiO NPs, as the single-component oxides of NiCo2O4. Our study may open up the possibility to make a great inﬂuence on the next generation of enzyme mimetics system.
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China. Electronic address: suli@htu.edu.cn.
authors.forename: Li
authors.initials: L
authors.lastname: Su
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Wenpei
authors.initials: W
authors.lastname: Dong
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Chengke
authors.initials: C
authors.lastname: Wu
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Yijun
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Ling
authors.initials: L
authors.lastname: Li
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Guojiang
authors.initials: G
authors.lastname: Mao
authors.affiliation: Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals, Key Laboratory of Green Chemical Media and Reactions, Ministry of Education, Henan Normal University, Xinxiang, Henan 453007, PR China; Henan Key Laboratory of Green Chemical Media and Reactions, School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang, Henan 453007, PR China.
authors.forename: Suling
authors.initials: S
authors.lastname: Feng
mesh_terms: D015374:Biosensing Techniques; D001786:Blood Glucose / Q000032:analysis*; D003035:Cobalt / Q000737:chemistry; D003124:Colorimetry; D006801:Humans; D006878:Hydroxides / Q000737:chemistry*; D009532:Nickel / Q000737:chemistry; D010088:Oxidoreductases; D010544:Peroxidases
chemical_list: D001786:Blood Glucose; D006878:Hydroxides; D003035:Cobalt; D009532:Nickel; D010088:Oxidoreductases; D010544:Peroxidases
keywords: Colorimetric biosensing; Electron spin resonance; Glucose detection; NiCo(2)O(4) mesoporous spheres; Peroxidase and oxidase-like activity