title: Enhanced production of curdlan by coupled fermentation system of Agrobacterium sp. ATCC 31749 and Trichoderma harzianum GIM 3.442.
abstract: A coupled fermentation system of Agrobacterium sp. ATCC 31749 and Trichoderma harzianum GIM 3.442 (AT-CFS) with wheat bran as the optimal nitrogen source was established for producing low-molecular-weight curdlan with high production, which can potentially reduce the cost of low-molecular-weight curdlan biosynthesis. The initial inoculate ratio, pH and the fermentation time were optimized. Compared with the curdlan from the single fermentation system of Agrobacterium sp. ATCC 31749 (A-SFS), the molecular weight (Mw) of the curdlan produced from AT-CFS decreased by 34.01% (from 110.85kDa to 73.15kDa), and the curdlan production (47.9g/L) and conversion rate of glucose to curdlan (0.60gg-1) increased by 119.93% and 36.36%, respectively. The results of RT-PCR showed high curdlan production in AT-CFS was highly correlated with aerobic respiration intensity and curdlan synthase activity. The structure of the curdlan from AT-CFS was the same as that from A-SFS.
authors.affiliation: Ministry of Education, Key Lab Carbohydrate Chemical and Biotechnology & School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: waitliangying@foxmail.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Jiangsu Rayguang Biotech Co. Ltd., Wuxi 214125, Jiangsu, China. Electronic address: zhanzhuli@yahoo.com.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Ministry of Education, Key Lab Carbohydrate Chemical and Biotechnology & School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: dinghan_evelyn@163.com.
authors.forename: Han
authors.initials: H
authors.lastname: Ding
authors.affiliation: Ministry of Education, Key Lab Carbohydrate Chemical and Biotechnology & School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: gaominjie@msn.com.
authors.forename: Minjie
authors.initials: M
authors.lastname: Gao
authors.affiliation: Ministry of Education, Key Lab Carbohydrate Chemical and Biotechnology & School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: zhiyong@jiangnan.edu.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: Ministry of Education, Key Lab Carbohydrate Chemical and Biotechnology & School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: kinowu@jiangnan.edu.cn.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Ministry of Education, Key Lab Carbohydrate Chemical and Biotechnology & School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China. Electronic address: xbzhan@yahoo.com.
authors.forename: Xiaobei
authors.initials: X
authors.lastname: Zhan
mesh_terms: D060054:Agrobacterium / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D014242:Trichoderma / Q000378:metabolism*; D047071:beta-Glucans / Q000378:metabolism*
chemical_list: D047071:beta-Glucans; C038459:curdlan
keywords: Coupled fermentation system; Curdlan; Molecular weight