title: Identification and Characterisation of a Novel Protein FIP-sch3 from Stachybotrys chartarum.
abstract: In this study, a novel FIP named FIP-sch3 has been identified and characterised. FIP-sch3 was identified in the ascomycete Stachybotrys chartarum, making it the second FIP to be identified outside the order of Basidiomycota. Recombinant FIP-sch3 (rFIP-shc3) was produced in Escherichia coli and purified using GST-affinity magnetic beads. The bioactive characteristics of FIP-sch3 were compared to those of well-known FIPs LZ-8 from Ganoderma lucidum and FIP-fve from Flammulina velutipes, which were produced and purified using the same method. The purified rFIP-sch3 exhibited a broad spectrum of anti-tumour activity in several types of tumour cells but had no cytotoxicity in normal human embryonic kidney 293 cells. Assays that were implemented to study these properties indicated that rFIP-sch3 significantly suppressed cell proliferation, induced apoptosis and inhibited cell migration in human lung adenocarcinoma A549 cells. The anti-tumour effects of rFIP-sch3 in A549 cells were comparable to those of rLZ-8, but they were significantly greater than those of rFIP-fve. Molecular assays that were built on real-time PCR further revealed potential mechanisms related to apoptosis and migration and that underlie phenotypic effects. These results indicate that FIP-shc3 has a unique anti-tumour bioactive profile, as do other FIPs, which provide a foundation for further studies on anti-tumour mechanisms. Importantly, this study also had convenient access to FIP-sch3 with potential human therapeutic applications.
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Leiming
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: School of Life Sciences, Tianjin University, Tianjin, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Fengzhong
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Xin
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D001203:Ascomycota / Q000737:chemistry*; D005656:Fungal Proteins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D006801:Humans; D009369:Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D013191:Stachybotrys / Q000737:chemistry*
chemical_list: D000970:Antineoplastic Agents; D005656:Fungal Proteins