title: Molecular detection of aminoglycoside-modifying enzyme genes in Acinetobacter baumannii clinical isolates.
abstract: Acinetobacter baumannii is a major opportunistic pathogen in healthcare settings worldwide. In Iran, there are only few reports on the prevalence of aminoglycoside resistance genes among A. baumannii isolates. The aim of this study was to investigate the existence of aminoglycoside-modifying enzyme (AME) genes from A. baumannii strains collected at a university teaching hospital in Iran. One hundred A. baumannii strains were collected between 2014 and 2015 from hospitalized patients at Loghman Hakim Hospital, Tehran, Iran. Antimicrobial susceptibility was determined by disk diffusion method according to the Clinical and Laboratory Standards Institute recommendations. The DNA was extracted using a kit obtained from Bioneer Co. (Korea) and was used as a template for polymerase chain reaction. The most active antimicrobial agent against these strains was colistin. The rate of extended-spectrum cephalosporin resistance was 97%. The aadA1, aadB, aac(6')-Ib, and aac(3)-IIa genes were found in 85%, 77%, 72%, and 68% of A. baumannii isolates, respectively. This study showed a high prevalence rate of AME genes in A. baumannii. This prevalence rate has explained that further aminoglycoside resistance genes may have role in the resistance of clinical isolates of A. baumannii. Therefore, control and treatment of serious infections caused by this opportunistic pathogen should be given more consideration.
authors.affiliation: 1 Department of Microbiology, School of Medicine, Iran University of Medical Sciences , Tehran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Heidary
authors.affiliation: 2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Salimi Chirani
authors.affiliation: 3 Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences , Ahvaz, Iran.
authors.forename: Saeed
authors.initials: S
authors.lastname: Khoshnood
authors.affiliation: 2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran.
authors.forename: Gita
authors.initials: G
authors.lastname: Eslami
authors.affiliation: 4 Department of Pilot Nanobiotechnology, Pasteur Institute of Iran , Tehran, Iran.
authors.forename: Seyyed Mohammad
authors.initials: SM
authors.lastname: Atyabi
authors.affiliation: 5 Department of Biology, Payame Noor University , Isfahan, Iran.
authors.forename: Habibollah
authors.initials: H
authors.lastname: Nazem
authors.affiliation: 5 Department of Biology, Payame Noor University , Isfahan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Fazilati
authors.affiliation: 2 Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences , Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Hashemi
authors.affiliation: 5 Department of Biology, Payame Noor University , Isfahan, Iran.
authors.forename: Saleh
authors.initials: S
authors.lastname: Soleimani
mesh_terms: D000151:Acinetobacter Infections / Q000382:microbiology*; D040981:Acinetobacter baumannii / Q000201:enzymology* / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000617:Aminoglycosides / Q000378:metabolism* / Q000494:pharmacology; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D002648:Child; D002675:Child, Preschool; D005260:Female; D006801:Humans; D007492:Iran; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged; D055815:Young Adult
chemical_list: D000617:Aminoglycosides; D000900:Anti-Bacterial Agents; D001426:Bacterial Proteins
keywords: Acinetobacter baumannii; Iran; acetyltransferases; adenyltransferases; aminoglycoside