title: Validation and Application of an Ultra High-Performance Liquid Chromatography Tandem Mass Spectrometry Method for Yuanhuacine Determination in Rat Plasma after Pulmonary Administration: Pharmacokinetic Evaluation of a New Drug Delivery System.
abstract: Yuanhuacine was found to have significant inhibitory activity against A-549 human lung cancer cells. However, there would be serious adverse toxicity effects after systemic administration of yuanhuacine, such as by oral and intravenous ways. In order to achieve better curative effect and to alleviate the adverse toxicity effects, we tried to deliver yuanhuacine directly into the lungs. Ultra high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was used to detect the analyte and IS. After extraction (ether:dichloromethane = 8:1), the analyte and IS were separated on a Waters BEH-C18column (100 mm × 2.1 mm, 1.7 μm) under a 5 min gradient elution using a mixture of acetonitrile and 0.1% formic acid aqueous solution as mobile phase at a flow rate of 0.3 mL/min. ESI positive mode was chosen for detection. The method was fully validated for its selectivity, accuracy, precision, stability, matrix effect, and extraction recovery. This new method for yuanhuacine concentration determination in rat plasma was reliable and could be applied for its preclinical and clinical monitoring purpose.
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. liman921005@163.com.|Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China. liman921005@163.com.
authors.forename: Man
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. liuxiao04_0@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. haocai_98@126.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Cai
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. 18260093567@163.com.
authors.forename: Zhichun
authors.initials: Z
authors.lastname: Shen
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. m18260092677_1@163.com.
authors.forename: Liu
authors.initials: L
authors.lastname: Xu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. liweidong0801@163.com.
authors.forename: Weidong
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. wuli87107@163.com.
authors.forename: Li
authors.initials: L
authors.lastname: Wu
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. dja@njutcm.edu.cn.|Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China. dja@njutcm.edu.cn.
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
authors.affiliation: School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China. czpcpu@Sina.com.|Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Nanjing 210023, China. czpcpu@Sina.com.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Chen
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid; D004224:Diterpenes / Q000008:administration & dosage / Q000493:pharmacokinetics*; D016503:Drug Delivery Systems; D004355:Drug Stability; D008168:Lung / Q000187:drug effects; D008175:Lung Neoplasms; D008297:Male; D010949:Plasma / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D004224:Diterpenes; C029912:Yuanhuacin
keywords: pharmacokinetics; pulmonary drug delivery; ultra high-performance liquid chromatography tandem mass spectrometry; yuanhuacine