title: Optical Properties of Europium Tetracycline Complexes in the Presence of High-Density Lipoproteins (HDL) Subfractions.
abstract: Standard lipoprotein measurements of triglycerides, total cholesterol, low-density lipoproteins (LDL), and high-density lipoproteins (HDL) fail to identify many lipoprotein abnormalities that contribute to cardiovascular heart diseases (CHD). Studies suggested that the presence of CHD is more strongly associated with the HDL subspecies than with total HDL cholesterol levels. The HDL particles can be collected in at least three subfractions, the HDL2b, HDL2a, and HDL3. More specifically, atherosclerosis is associated with low levels of HDL2. In this work, the optical spectroscopic properties of europium tetracycline (EuTc) complex in the presence of different HDL subspecies was studied. The results show that the europium spectroscopic properties in the EuTc complex are influenced by sizes and concentrations of subclasses. Eu3+emission intensity and lifetime can discriminate the subfractions HDL3 and HDL2b.
authors.affiliation: 1 Centro de Lasers e Aplicações, IPEN/CNEN, São Paulo, SP, Brazil.
authors.forename: Letícia Bonfante
authors.initials: LB
authors.lastname: Sicchieri
authors.affiliation: 2 Instituto de Física, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Andrea Moreira
authors.initials: AM
authors.lastname: Monteiro
authors.affiliation: 2 Instituto de Física, Universidade de São Paulo, São Paulo, SP, Brazil.
authors.forename: Antônio Martins
authors.initials: AM
authors.lastname: Figueiredo Neto
authors.affiliation: 1 Centro de Lasers e Aplicações, IPEN/CNEN, São Paulo, SP, Brazil.
authors.forename: Laércio
authors.initials: L
authors.lastname: Gomes
authors.affiliation: 1 Centro de Lasers e Aplicações, IPEN/CNEN, São Paulo, SP, Brazil.|3 Depto de Ciências Exatas e da Terra, Universidade Federal de São Paulo (Brazil), Diadema, SP, Brazil.
authors.forename: Lilia Coronato
authors.initials: LC
authors.lastname: Courrol
mesh_terms: D005063:Europium / Q000737:chemistry*; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood* / Q000737:chemistry* / Q000145:classification; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013754:Tetracyclines / Q000737:chemistry*
chemical_list: D008075:Lipoproteins, HDL; D013754:Tetracyclines; D005063:Europium
keywords: HDL; High-density lipoprotein subfractions; cardiovascular diseases; europium tetracycline; fluorescence lifetime