title: Fluorescent Labeling and Biodistribution of Latex Nanoparticles Formed by Surfactant-Free RAFT Emulsion Polymerization.
abstract: The authors report the preparation of a novel range of functional polyacrylamide stabilized polystyrene nanoparticles, obtained by surfactant-free reversible addition-fragmentation chain transfer (RAFT) emulsion polymerization, their fluorescent tagging, cellular uptake, and biodistribution. The authors show the versatility of the RAFT emulsion process for the design of functional nanoparticles of well-defined size that can be used as drug delivery vectors. Functionalization with a fluorescent tag offers a useful visualization tool for tracing, localization, and clearance studies of these carriers in biological models. The studies are carried out by labeling the sterically stabilized latex particles chemically with rhodamine B. The fluorescent particles are incubated in a healthy human renal proximal tubular cell line model, and intravenously injected into a mouse model. Cellular localization and biodistribution of these particles on the biological models are explored.
authors.affiliation: Key Centre for Polymers & Colloids, School of Chemistry, The University of Sydney, Building F11, NSW, 2006, Australia.
authors.forename: Cheuk Ka
authors.initials: CK
authors.lastname: Poon
authors.affiliation: Kolling Institute of Medical Research, Royal North Shore Hospital and The University of Sydney, St Leonards, NSW, 2065, Australia.
authors.forename: Owen
authors.initials: O
authors.lastname: Tang
authors.affiliation: Kolling Institute of Medical Research, Royal North Shore Hospital and The University of Sydney, St Leonards, NSW, 2065, Australia.
authors.forename: Xin-Ming
authors.initials: XM
authors.lastname: Chen
authors.affiliation: Key Centre for Polymers & Colloids, School of Chemistry, The University of Sydney, Building F11, NSW, 2006, Australia.
authors.forename: Byung
authors.initials: B
authors.lastname: Kim
authors.affiliation: Department of Chemistry, The University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hartlieb
authors.affiliation: Kolling Institute of Medical Research, Royal North Shore Hospital and The University of Sydney, St Leonards, NSW, 2065, Australia.
authors.forename: Carol A
authors.initials: CA
authors.lastname: Pollock
authors.affiliation: Key Centre for Polymers & Colloids, School of Chemistry, The University of Sydney, Building F11, NSW, 2006, Australia.
authors.forename: Brian S
authors.initials: BS
authors.lastname: Hawkett
authors.affiliation: Key Centre for Polymers & Colloids, School of Chemistry, The University of Sydney, Building F11, NSW, 2006, Australia.|Department of Chemistry, The University of Warwick, Coventry, CV4 7AL, UK.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Perrier
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry; D000818:Animals; D001692:Biological Transport; D002460:Cell Line; D004337:Drug Carriers; D004655:Emulsions; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D007275:Injections, Intravenous; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008863:Microspheres; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010316:Particle Size; D058105:Polymerization; D011137:Polystyrenes / Q000737:chemistry; D012235:Rhodamines / Q000737:chemistry*; D013194:Staining and Labeling / Q000379:methods*; D014018:Tissue Distribution; D000099166:Polyacrylamides
chemical_list: D000180:Acrylic Resins; D004337:Drug Carriers; D004655:Emulsions; D005456:Fluorescent Dyes; D011137:Polystyrenes; D012235:Rhodamines; D000099166:Polyacrylamides; C029773:rhodamine B
keywords: RAFT emulsion; biodistribution; cell uptake; nanoparticles