title: Image-Based Analysis of Intracellular Tau Aggregation by Using Tau-BiFC Cell Model.
abstract: Abnormal tau aggregation is a pathological hallmark of neurodegenerative disease classified as tauopathy. Preventing tau aggregation becomes an important therapeutic strategy to cure tau-mediated neurodegeneration. Here, we describe a method to investigate intracellular tau aggregation by using a recently developed tau aggregation cell-based model named tau-BiFC. High-throughput and high-contents screening method for quantifying intracellular tau aggregation would expedite the discovery of drugs that inhibit tau aggregation.
authors.affiliation: Convergence Research Center for Diagnosis, Treatment and Care System of Dementia, Brain Science Institute, Korea Institute of Science and Technology (KIST), Hwarangro 14-gil 5, Seongbuk-gu, Seoul, 136-791, South Korea.
authors.forename: Sungsu
authors.initials: S
authors.lastname: Lim
authors.affiliation: Convergence Research Center for Diagnosis, Treatment and Care System of Dementia, Brain Science Institute, Korea Institute of Science and Technology (KIST), Hwarangro 14-gil 5, Seongbuk-gu, Seoul, 136-791, South Korea.|Department of Biotechnology, Translational Research Center for Protein Function Control, College of Life Science and Biotechnology, Yonsei University, Seoul, 120-749, South Korea.
authors.forename: Dohee
authors.initials: D
authors.lastname: Kim
authors.affiliation: Center for neuro-medicine, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul, 136-791, South Korea.
authors.forename: Dong Jin
authors.initials: DJ
authors.lastname: Kim
authors.affiliation: Convergence Research Center for Diagnosis, Treatment and Care System of Dementia, Brain Science Institute, Korea Institute of Science and Technology (KIST), Hwarangro 14-gil 5, Seongbuk-gu, Seoul, 136-791, South Korea. yunkyungkim@kist.re.kr.|Biological Chemistry, University of Science and Technology (UST), Daejeon, 305-333, South Korea. yunkyungkim@kist.re.kr.
authors.forename: Yun Kyung
authors.initials: YK
authors.lastname: Kim
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques; D005576:Colforsin / Q000378:metabolism; D057809:HEK293 Cells; D006801:Humans; D057054:Molecular Imaging / Q000379:methods*; D010766:Phosphorylation; D012984:Software; D016875:tau Proteins / Q000032:analysis* / Q000378:metabolism*
chemical_list: D016875:tau Proteins; D005576:Colforsin
keywords: Cell image analysis; Forskolin; Harmony® software; High content imaging system (Operetta™); Tau aggregation; Tau phosphorylation; Tau protein; Tau-BiFC cells