title: Involvement of viperin in prevention of intrauterine transmission of hepatitis B virus.
abstract: The aim of this study was to explore the role of viperin in the prevention of intrauterine infection of hepatitis B virus (HBV). Placental samples were collected from seven HBV-positive pregnant women with their infants infected via intrauterine transmission (infected group), 30 HBV-positive women with non-infected infants (non-infected group), and 30 HBV-negative women (controls). The expression of viperin in placenta was analyzed with immunohistochemistry and Western blotting. The expression of viperin of placental trophoblast cell line Swan71 was determined after exposed to HBV. Viperin was localized to syncytiotrophoblast, and there was a significant difference in placental viperin levels among control, non-infected group and infected group (F = 12.824, p < 0.001). The expression of viperin was significantly higher in non-infected group than in control (p = 0.019) and in infected group (p < 0.001), and viperin expression was significantly lower in infected group than in control (p = 0.001). The exposure to HBV significantly increased the expression of viperin in Swan 71 (p < 0.001). Exposure to HBV up-regulates viperin expression in vivo and in vitro in placental trophoblast, and lack of this up-regulation is associated with intrauterine transmission of HBV.
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Bai
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Huiyan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Liuxia
authors.initials: L
authors.lastname: Wan
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Wang
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Hanzhi
authors.initials: H
authors.lastname: Wang
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Xiaofu
authors.initials: X
authors.lastname: Yang
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Women's Hospital, School of Medicine, Zhejiang University, Zhejiang, China.|Key Laboratory of Women's Reproductive Health of Zhejiang Province, Zhejiang, China.|Key Laboratory of Reproductive Genetics of the Ministry of Education, Zhejiang, China.
authors.forename: Minyue
authors.initials: M
authors.lastname: Dong
mesh_terms: D000328:Adult; D015153:Blotting, Western; D002460:Cell Line; D005260:Female; D006509:Hepatitis B / Q000276:immunology* / Q000635:transmission*; D006515:Hepatitis B virus / Q000276:immunology*; D006801:Humans; D007150:Immunohistochemistry; D007231:Infant, Newborn; D018445:Infectious Disease Transmission, Vertical / Q000517:prevention & control*; D008297:Male; D044925:Oxidoreductases Acting on CH-CH Group Donors; D010920:Placenta / Q000737:chemistry / Q000473:pathology*; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000276:immunology*; D011506:Proteins / Q000378:metabolism*; D014327:Trophoblasts / Q000737:chemistry; D000090123:Viperin Protein
chemical_list: D044925:Oxidoreductases Acting on CH-CH Group Donors; D011506:Proteins; C444427:RSAD2 protein, human; D000090123:Viperin Protein
keywords: Hepatitis B virus; intrauterine transmission; viperin