title: Impact of storage conditions on the urinary metabolomics fingerprint.
abstract: Urine stability during storage is essential in metabolomics to avoid misleading conclusions or erroneous interpretations. Facing the lack of comprehensive studies on urine metabolome stability, the present work performed a follow-up of potential modifications in urinary chemical profile using LC-HRMS on the basis of two parameters: the storage temperature (+4 °C, -20 °C, -80 °C and freeze-dried stored at -80 °C) and the storage duration (5-144 days). Both HILIC and RP chromatographies have been implemented in order to globally monitor the urinary metabolome. Using an original data processing associated to univariate and multivariate data analysis, our study confirms that chemical profiles of urine samples stored at +4 °C are very rapidly modified, as observed for instance for compounds such as:N-acetyl Glycine, Adenosine, 4-Amino benzoic acid, N-Amino diglycine, creatine, glucuronic acid, 3-hydroxy-benzoic acid, pyridoxal, l-pyroglutamic acid, shikimic acid, succinic acid, thymidine, trigonelline and valeryl-carnitine, while it also demonstrates that urine samples stored at -20 °C exhibit a global stability over a long period with no major modifications compared to -80 °C condition. This study is the first to investigate long term stability of urine samples and report potential modifications in the urinary metabolome, using both targeted approach monitoring individually a large number (n > 200) of urinary metabolites and an untargeted strategy enabling assessing for global impact of storage conditions.
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes, F-44307, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Laparre
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes, F-44307, France.
authors.forename: Zied
authors.initials: Z
authors.lastname: Kaabia
authors.affiliation: School of Biological Sciences, Queen's University Belfast (QUB), Medical Biology Centre, 97 Lisburn Road, Belfast, BT9 7BL, United Kingdom.
authors.forename: Mark
authors.initials: M
authors.lastname: Mooney
authors.affiliation: Irish Equine Center, Johnstown, Naas, County Kildare, W91 RH93, Ireland.
authors.forename: Tom
authors.initials: T
authors.lastname: Buckley
authors.affiliation: IDLS Irish Equine Centre, Johnstown, Naas, County Kildare, W91 RH93, Ireland.
authors.forename: Mark
authors.initials: M
authors.lastname: Sherry
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes, F-44307, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Le Bizec
authors.affiliation: LUNAM Université, Oniris, Laboratoire d'Etude des Résidus et Contaminants dans les Aliments (LABERCA), Nantes, F-44307, France. Electronic address: gaud.dervilly@oniris-nantes.fr.
authors.forename: Gaud
authors.initials: G
authors.lastname: Dervilly-Pinel
mesh_terms: D000818:Animals; D002417:Cattle; D003080:Cold Temperature; D005260:Female; D005612:Freeze Drying; D055442:Metabolome; D055432:Metabolomics; D015999:Multivariate Analysis; D013048:Specimen Handling / Q000379:methods*; D014556:Urine / Q000737:chemistry*
keywords: Metabolite profiling; Metabolites data base; Stability; Storage; Urine