title: Resistant starch/pectin free-standing films reinforced with nanocellulose intended for colonic methotrexate release.
abstract: Although resistant starch/pectin (RS/P) films have previously displayed suitable properties for colon-specific drug delivery, nanocomposite films were developed aiming to enhance physicochemical, thermal, mechanical and barrier properties, as well as the low oral bioavailability of methotrexate (MTX). FEG-SEM micrographs of nanocomposite films showed different interaction patterns occurring among nanocellulose and RS/P. The nanofiller addition led to an increase in the thermal stability, probably due to its interaction with RS crystalline double helices. Results also displayed an improvement of the puncture strength, while barrier properties revealed a low water vapor permeability. Ex vivo bioadhesion test displayed the nanocomposites films to interact strongly with porcine gastrointestinal mucosa. In vitro drug release studies showed that the films developed enhanced the drug dissolution rates with approximately 80% of MTX release in 150min, suggesting the potential of these materials as a poor solubility drugs carrier, which constitutes an important tool for enhancing oral bioavailability.
authors.affiliation: Interdisciplinary Laboratory of Advanced Materials, Centro de Ciências da Natureza - CNN, Universidade Federal do Piauí - UFPI, 64049-550 Teresina, PI, Brazil; Universidade de Araraquara - UNIARA, 14801-320 Araraquara, SP, Brazil. Electronic address: abagliottim@hotmail.com.
authors.forename: Andréia B
authors.initials: AB
authors.lastname: Meneguin
authors.affiliation: Department of Drugs and Pharmaceuticals, Faculty of Pharmaceutical Sciences, São Paulo State University-UNESP, 14800-903 Araraquara, SP, Brazil. Electronic address: curybsf@fcfar.unesp.br.
authors.forename: Beatriz Stringhetti
authors.initials: BS
authors.lastname: Ferreira Cury
authors.affiliation: Department of Drugs and Pharmaceuticals, Faculty of Pharmaceutical Sciences, São Paulo State University-UNESP, 14800-903 Araraquara, SP, Brazil. Electronic address: aline.martinsdossantos@yahoo.com.br.
authors.forename: Aline M
authors.initials: AM
authors.lastname: Dos Santos
authors.affiliation: Institute of Chemistry, São Paulo State University - UNESP, 14801-970 Araraquara, SP, Brazil. Electronic address: fazafranco@yahoo.com.br.
authors.forename: Douglas Faza
authors.initials: DF
authors.lastname: Franco
authors.affiliation: Institute of Chemistry, São Paulo State University - UNESP, 14801-970 Araraquara, SP, Brazil; Universidade de Araraquara - UNIARA, 14801-320 Araraquara, SP, Brazil. Electronic address: hernane.barud@gmail.com.
authors.forename: Hernane S
authors.initials: HS
authors.lastname: Barud
authors.affiliation: Interdisciplinary Laboratory of Advanced Materials, Centro de Ciências da Natureza - CNN, Universidade Federal do Piauí - UFPI, 64049-550 Teresina, PI, Brazil. Electronic address: edsonfilho@ufpi.edu.br.
authors.forename: Edson C
authors.initials: EC
authors.lastname: da Silva Filho
mesh_terms: D000818:Animals; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D007413:Intestinal Mucosa / Q000187:drug effects; D008727:Methotrexate / Q000008:administration & dosage*; D053761:Nanocomposites; D010368:Pectins / Q000737:chemistry*; D010539:Permeability; D012995:Solubility; D013213:Starch / Q000737:chemistry*; D013552:Swine
chemical_list: D004337:Drug Carriers; D010368:Pectins; D013213:Starch; D008727:Methotrexate
keywords: Cellulose nanofiber; Free-standing films; Methotrexate; Nanocellulose; Pectin; Resistant starch