title: Eichhornia crassipes: Agro-waster for a novel thermostable laccase production by Pycnoporus sanguineus SYBC-L1.
abstract: The aim of this study was to explore the utilization of an intractable waster of Eichhornia crassipes in laccase production by Pycnoporus sanguineus SYBC-L1. E. crassipes as the sole carbon and nitrogen source was confirmed to produce laccase (7.26 U/g dry substrate). The fermentation medium for the maximum enzyme production was optimized and the laccase was then purified and characterized. The optimized culture medium was 25.1% E. crassipes, 13.9% sawdust, 1.5 mM CuSO4, 40 μM gallic acid, 65% moisture content and initial pH 6.0. A maximum laccase activity of 32.02 U/g dry substrate was detected at 9th day, which was 4.5-fold compared to the initial medium. The molecular mass of the purified Lac-S was 58.4 kDa. The optimum activity of Lac-S for DMP was at pH 3.0 and 70°C. Lac-S showed not only high catalytic activities at low temperature, but also good stabilities toward pH and temperature. The residual catalytic activities of Lac-S were 30%, 40% and 50% at 0°C, 10°C and 20°C, and the half-lives at 50°C, 60°C and 70°C were 21.7, 9.7 and 1.5 h, respectively. The results provide a significant basis for E. crassipes further utilization and Lac-S specific application in harsh industry.
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China.
authors.forename: Zhixin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Bioengineering Department, Huanghuai University, Zhumadian, Henan 250014, China.
authors.forename: Jiayang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China.
authors.forename: Yawei
authors.initials: Y
authors.lastname: Ning
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China.
authors.forename: Xiangru
authors.initials: X
authors.lastname: Liao
authors.affiliation: Research Center for Fermentation Engineering of Hebei, College of Bioscience and Bioengineering, Hebei University of Science and Technology, Shijiazhuang, Hebei 050018, China. Electronic address: ymjia0311@outlook.com.
authors.forename: Yingmin
authors.initials: Y
authors.lastname: Jia
mesh_terms: D000383:Agriculture; D002244:Carbon / Q000737:chemistry / Q000378:metabolism; D003080:Cold Temperature; D003470:Culture Media / Q000737:chemistry; D031806:Eichhornia / Q000737:chemistry / Q000378:metabolism*; D004795:Enzyme Stability; D005285:Fermentation; D042845:Laccase / Q000096:biosynthesis*; D008031:Lignin / Q000737:chemistry / Q000378:metabolism; D009584:Nitrogen / Q000737:chemistry / Q000378:metabolism; D055494:Pycnoporus / Q000201:enzymology* / Q000378:metabolism; D062611:Solid Waste; D013696:Temperature; D014934:Wood
chemical_list: D003470:Culture Media; D062611:Solid Waste; C036909:lignocellulose; D002244:Carbon; D008031:Lignin; D042845:Laccase; D009584:Nitrogen
keywords: Eichhornia crassipes; Laccase; Pycnoporus sanguineus; Solid-state fermentation; Thermostability