title: Top-Down Mass Spectrometry: Proteomics to Proteoforms.
abstract: This chapter highlights many of the fundamental concepts and technologies in the field of top-down mass spectrometry (TDMS), and provides numerous examples of contributions that TD is making in biology, biophysics, and clinical investigations. TD workflows include variegated steps that may include non-specific or targeted preparative strategies, orthogonal liquid chromatography techniques, analyte ionization, mass analysis, tandem mass spectrometry (MS/MS) and informatics procedures. This diversity of experimental designs has evolved to manage the large dynamic range of protein expression and diverse physiochemical properties of proteins in proteome investigations, tackle proteoform microheterogeneity, as well as determine structure and composition of gas-phase proteins and protein assemblies.
authors.affiliation: Computational and Systems Biology & Biomedical Engineering Graduate Programs, University of Texas Southwestern Medical Center, Dallas, TX, USA. Steven.Patrie@UTSouthwestern.edu.|Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA. Steven.Patrie@UTSouthwestern.edu.|Department of Bioengineering, University of Texas at Dallas, Richardson, TX, USA. Steven.Patrie@UTSouthwestern.edu.
authors.forename: Steven M
authors.initials: SM
authors.lastname: Patrie
mesh_terms: D000818:Animals; D002845:Chromatography / Q000379:methods*; D019295:Computational Biology; D030562:Databases, Protein; D057166:High-Throughput Screening Assays; D006801:Humans; D013058:Mass Spectrometry / Q000379:methods*; D011506:Proteins / Q000032:analysis*; D020543:Proteome; D040901:Proteomics / Q000379:methods*; D057188:Workflow
chemical_list: D011506:Proteins; D020543:Proteome
keywords: Alternative splicing; Bottom-up; Charge reduction; Chromatography; Data-dependent; Data-independent; Electrospray ionization; FT-ICR; Fourier transform; Hydrogen/deuterium exchange; Informatics; Label-free quantitation; Mass spectrometry; Membrane proteins; Native MS; Orbitrap; Post-translational modifications; Protein; Proteoform; Proteomics; Quadrupole ion trap; Reversed-phase; Review; SILAC; SNPs; Supercharging; Tandem MS; Time-of-flight; Top-down