title: Clinical potential of miRNA-221 as a novel prognostic biomarker for hepatocellular carcinoma.
abstract: miRNA-221 is one of the over 700 kinds of currently known microRNAs (miRNAs) and is up-regulated in multiple tumors, suggesting that it may be a potential carcinogenic miRNA. Few studies have explored the relationship between miRNA-221 and hepatocellular carcinoma (HCC). We performed real-time quantitative polymerase chain reaction (qPCR) to detect miRNA-221 expression in HCC and para-carcinoma tissues and to explore the relationship between abnormal expression of miRNA-221 and clinicopathological features of HCC patients. miRNA-221 expression was significantly higher in HCC tissues than in adjacent tissues (P < 0.001). We analyzed the relationship between miRNA-221 expression level and clinicopathological characteristics of HCC patients. Our results suggested that miRNA-221 expression level was closely related to tumor stage (P = 0.012), number of tumor nodes (P = 0.018), and microvascular invasion (P = 0.010) in HCC patients. The results of survival analysis suggested that HCC patients with up-regulated miRNA-221 expression had a shorter survival time. The high miRNA-221 expression indicates the poor prognosis of HCC patients; thus, miRNA-221 can be regarded an important molecular marker for HCC prognosis.
authors.affiliation: Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, Urumqi, Xinjiang, China.|Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, Urumqi, Xinjiang, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Chen
authors.affiliation: Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, Urumqi, Xinjiang, China.|Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, Urumqi, Xinjiang, China.
authors.forename: Xin-Feng
authors.initials: XF
authors.lastname: Li
authors.affiliation: Department of Surgery, The Sixth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.|Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, Urumqi, Xinjiang, China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Fu
authors.affiliation: Department of Oncology, Urumqi General Hospital of Lanzhou Military Command of PLA, Urumqi, Xinjiang, China.
authors.forename: Jian-Guo
authors.initials: JG
authors.lastname: Huang
authors.affiliation: Department of Internal Medicine, Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China.
authors.forename: Shun-E
authors.initials: SE
authors.lastname: Yang
mesh_terms: D000328:Adult; D014408:Biomarkers, Tumor / Q000235:genetics*; D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D011379:Prognosis; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs; C525709:MIR221, human
keywords: MicroRNA; carcinoma; hepatocellular; prognosis; real-time qPCR