title: The Effect of Selenium Supplementation on Glucose Homeostasis and the Expression of Genes Related to Glucose Metabolism.
abstract: The aim of the study was to evaluate the effect of selenium supplementation on the expression of genes associated with glucose metabolism in humans, in order to explain the unclear relationship between selenium and the risk of diabetes. For gene expression analysis we used archival samples of cDNA from 76 non-diabetic subjects supplemented with selenium in the previous study. The supplementation period was six weeks and the daily dose of selenium was 200 µg (as selenium yeast). Blood for mRNA isolation was collected at four time points: before supplementation, after two and four weeks of supplementation, and after four weeks of washout. The analysis included 15 genes encoding selected proteins involved in insulin signaling and glucose metabolism. In addition, HbA1c and fasting plasma glucose were measured at three and four time points, respectively. Selenium supplementation was associated with a significantly decreased level of HbA1c but not fasting plasma glucose (FPG) and significant down-regulation of seven genes:INSR,ADIPOR1,LDHA,PDHA,PDHB,MYC, andHIF1AN. These results suggest that selenium may affect glycemic control at different levels of regulation, linked to insulin signaling, glycolysis, and pyruvate metabolism. Further research is needed to investigate mechanisms of such transcriptional regulation and its potential implication in direct metabolic effects.
authors.affiliation: Nofer Institute of Occupational Medicine, Department of Toxicology and Carcinogenesis, Sw. Teresy 8 Street, 91-348 Lodz, Poland. ewa.jablonska@imp.lodz.pl.
authors.forename: Ewa
authors.initials: E
authors.lastname: Jablonska
authors.affiliation: Nofer Institute of Occupational Medicine, Department of Toxicology and Carcinogenesis, Sw. Teresy 8 Street, 91-348 Lodz, Poland. edyta.reszka@imp.lodz.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Reszka
authors.affiliation: Nofer Institute of Occupational Medicine, Department of Biological and Environmental Monitoring, Sw. Teresy 8 Street, 91-348 Lodz, Poland. jolanta.gromadzinska@imp.lodz.pl.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Gromadzinska
authors.affiliation: Nofer Institute of Occupational Medicine, Department of Toxicology and Carcinogenesis, Sw. Teresy 8 Street, 91-348 Lodz, Poland. edyta.wieczorek@imp.lodz.pl.
authors.forename: Edyta
authors.initials: E
authors.lastname: Wieczorek
authors.affiliation: Nofer Institute of Occupational Medicine, Department of Biological and Environmental Monitoring, Sw. Teresy 8 Street, 91-348 Lodz, Poland. magdalena.krol@imp.lodz.pl.
authors.forename: Magdalena B
authors.initials: MB
authors.lastname: Krol
authors.affiliation: European Institute of Oncology, Division of Epidemiology and Biostatistics, via Ripamonti 435, Milan 20139, Italy. sara.raimondi@ieo.it.
authors.forename: Sara
authors.initials: S
authors.lastname: Raimondi
authors.affiliation: The Medical University of Bialystok, Department of Bromatoloy, A. Mickiewicza 2D Street, 15-222 Bialystok, Poland. katarzyna.socha@umb.edu.pl.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Socha
authors.affiliation: The Medical University of Bialystok, Department of Bromatoloy, A. Mickiewicza 2D Street, 15-222 Bialystok, Poland. borawska@umb.edu.pl.
authors.forename: Maria H
authors.initials: MH
authors.lastname: Borawska
authors.affiliation: Nofer Institute of Occupational Medicine, Department of Biological and Environmental Monitoring, Sw. Teresy 8 Street, 91-348 Lodz, Poland. wojciech.wasowicz@imp.lodz.pl.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Wasowicz
mesh_terms: D000328:Adult; D015703:Antigens, CD / Q000097:blood / Q000378:metabolism; D001786:Blood Glucose / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism; D019587:Dietary Supplements; D015536:Down-Regulation / Q000187:drug effects; D005215:Fasting / Q000097:blood; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D016259:Genes, myc / Q000187:drug effects; D006442:Glycated Hemoglobin / Q000032:analysis / Q000187:drug effects; D006706:Homeostasis; D006801:Humans; D045022:Lactate Dehydrogenases / Q000097:blood / Q000378:metabolism; D008297:Male; D006899:Mixed Function Oxygenases / Q000097:blood / Q000378:metabolism; D030481:Pyruvate Dehydrogenase (Lipoamide) / Q000097:blood / Q000378:metabolism; D012333:RNA, Messenger / Q000097:blood / Q000302:isolation & purification; D011972:Receptor, Insulin / Q000097:blood / Q000378:metabolism; D054419:Receptors, Adiponectin / Q000097:blood / Q000378:metabolism; D012097:Repressor Proteins / Q000097:blood / Q000378:metabolism; D012643:Selenium / Q000008:administration & dosage / Q000494:pharmacology*; D014131:Trace Elements / Q000008:administration & dosage / Q000494:pharmacology*
chemical_list: D015703:Antigens, CD; D001786:Blood Glucose; D006442:Glycated Hemoglobin; D045022:Lactate Dehydrogenases; D006899:Mixed Function Oxygenases; D030481:Pyruvate Dehydrogenase (Lipoamide); D012333:RNA, Messenger; D011972:Receptor, Insulin; D054419:Receptors, Adiponectin; D012097:Repressor Proteins; D012643:Selenium; D014131:Trace Elements; C475418:ADIPOR1 protein, human; C517652:hemoglobin A1c protein, human; C439790:HIF1AN protein, human; C435941:INSR protein, human
keywords: energy metabolism; fasting plasma glucose; gene expression; glucose metabolism; glycated hemoglobin; glycolysis; insulin signaling; pyruvate metabolism; selenium