title: Label-Free Aptasensors for the Detection of Mycotoxins.
abstract: Various methodologies have been reported in the literature for the qualitative and quantitative monitoring of mycotoxins in food and feed samples. Based on their enhanced specificity, selectivity and versatility, bio-affinity assays have inspired many researchers to develop sensors by exploring bio-recognition phenomena. However, a significant problem in the fabrication of these devices is that most of the biomolecules do not generate an easily measurable signal upon binding to the target analytes, and signal-generating labels are required to perform the measurements. In this context, aptamers have been emerged as a potential and attractive bio-recognition element to design label-free aptasensors for various target analytes. Contrary to other bioreceptor-based approaches, the aptamer-based assays rely on antigen binding-induced conformational changes or oligomerization states rather than binding-assisted changes in adsorbed mass or charge. This review will focus on current designs in label-free conformational switchable design strategies, with a particular focus on applications in the detection of mycotoxins.
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. a.rhouati@ensbiotech.edu.dz.|Ecole Nationale Supérieure de Biotechnologie, Constantine 25100, Algeria. a.rhouati@ensbiotech.edu.dz.
authors.forename: Amina
authors.initials: A
authors.lastname: Rhouati
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. gaelle.catanante@univ-perp.fr.
authors.forename: Gaelle
authors.initials: G
authors.lastname: Catanante
authors.affiliation: Technological Chemistry Department, Federal University of Maranhão, CCET/UFMA, Av. Portugueses, Cidade Universitária do Canga, 65080-040 São Luis, Brazil. gilvanda.nunes@hotmail.com.
authors.forename: Gilvanda
authors.initials: G
authors.lastname: Nunes
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. akhtarhayat@ciitlahore.edu.pk.|Interdisciplinary Research Centre in Biomedical Materials (IRCBM) COMSATS Institute of Information Technology (CIIT), Lahore 54000, Pakistan. akhtarhayat@ciitlahore.edu.pk.
authors.forename: Akhtar
authors.initials: A
authors.lastname: Hayat
authors.affiliation: BAE Laboratory, Université de Perpignan Via Domitia, 52 Avenue Paul Alduy, Perpignan 66860, France. jlmarty@univ-perp.fr.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Marty
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation*; D003124:Colorimetry; D021881:DNA, Catalytic / Q000378:metabolism; D009183:Mycotoxins / Q000032:analysis*; D013194:Staining and Labeling
chemical_list: D052157:Aptamers, Nucleotide; D021881:DNA, Catalytic; D009183:Mycotoxins
keywords: aptamer; conformational changes; current trends; label free detection; mycotoxins