title: A rapid and precise method for measuring plasma apoE-rich HDL using polyethylene glycol and cation-exchange chromatography: a pilot study on the clinical significance of apoE-rich HDL measurements.
abstract: BACKGROUND
  High-density lipoprotein (HDL) containing apolipoprotein E (apoE-rich HDL) represents only a small portion of plasma HDL. Reliable methods for determining and isolating apoE-rich HDL have not been well studied.
  
  
  METHODS
  We established a novel analytical method for apoE-rich HDL using polyethylene glycol and a cation-exchange column (PEG-column method). Furthermore, we examined biochemical correlates of apoE-rich HDL-cholesterol (HDL-C) in 36 patients who underwent coronary computed tomographic angiography.
  
  
  RESULTS
  Our PEG-column method demonstrated high reproducibility (coefficient of variation <3.52%) and linearity up to 15mg/dl for apoE-rich HDL-C concentrations. Isolated apoE-rich HDL exhibited a larger diameter (14.8nm) than apoE-poor HDL (10.8nm) and contained both apoE and apoA-I. ApoE-rich HDL-C concentrations correlated significantly with triglycerides (rs=-0.646), LDL size (rs=0.472), adiponectin (rs=0.476), and other lipoprotein components. No significant correlation was obtained with the coronary calcium score. Multiple regression analysis revealed that plasma triglycerides and adiponectin concentrations remained significant independent predictors of apoE-rich (adjusted R2=0.486) but not apoE-poor HDL-C.
  
  
  CONCLUSIONS
  The PEG-column method demonstrated, to various degrees, significant correlations between HDL subfractions and several lipid-related biomarkers involved in an atherogenic lipoprotein profile. Our separation technique for apoE-rich HDL is useful to clarify the role of apoE-rich HDL in atherosclerosis.
authors.affiliation: Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Ikeda
authors.affiliation: Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan.
authors.forename: Ryoko
authors.initials: R
authors.lastname: Shinohata
authors.affiliation: Okayama Heart Clinic, Okayama, Japan.
authors.forename: Masaaki
authors.initials: M
authors.lastname: Murakami
authors.affiliation: Okayama Heart Clinic, Okayama, Japan.
authors.forename: Kazuyoshi
authors.initials: K
authors.lastname: Hina
authors.affiliation: Okayama Heart Clinic, Okayama, Japan.
authors.forename: Shigeshi
authors.initials: S
authors.lastname: Kamikawa
authors.affiliation: Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hirohata
authors.affiliation: Okayama Heart Clinic, Okayama, Japan.
authors.forename: Shozo
authors.initials: S
authors.lastname: Kusachi
authors.affiliation: Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan.
authors.forename: Arisa
authors.initials: A
authors.lastname: Tamura
authors.affiliation: Department of Medical Technology, Okayama University Graduate School of Health Sciences, Okayama, Japan. Electronic address: usuis@md.okayama-u.ac.jp.
authors.forename: Shinichi
authors.initials: S
authors.lastname: Usui
mesh_terms: D052242:Adiponectin / Q000097:blood; D001057:Apolipoproteins E / Q000097:blood*; D015415:Biomarkers / Q000097:blood*; D002021:Buffers; D002412:Cations; D011232:Chemical Precipitation; D002852:Chromatography, Ion Exchange / Q000295:instrumentation / Q000379:methods*; D000072226:Computed Tomography Angiography; D005260:Female; D006801:Humans; D008075:Lipoproteins, HDL / Q000097:blood*; D008274:Magnesium / Q000737:chemistry; D008297:Male; D010316:Particle Size; D010865:Pilot Projects; D011092:Polyethylene Glycols / Q000737:chemistry; D015203:Reproducibility of Results; D014280:Triglycerides / Q000097:blood; D055815:Young Adult
chemical_list: C499918:ADIPOQ protein, human; D052242:Adiponectin; D001057:Apolipoproteins E; D015415:Biomarkers; D002021:Buffers; D002412:Cations; D008075:Lipoproteins, HDL; D014280:Triglycerides; C025121:apolipoprotein E-rich HDL; D011092:Polyethylene Glycols; D008274:Magnesium
keywords: Atherogenic lipoprotein; Chromatography; HDL subfraction