title: Protein-Bound Polysaccharide from Corbicula fluminea Inhibits Cell Growth in MCF-7 and MDA-MB-231 Human Breast Cancer Cells.
abstract: A novel protein-bound polysaccharide, CFPS-1, isolated from Corbicula fluminea, is composed predominantly of mannose (Man) and glucose (Glc) in a molar ratio of 3.1:12.7. The polysaccharide, with an average molecular weight of about 283 kDa, also contains 10.8% protein. Atomic force microscopy, high-performance liquid chromatography, Fourier transform infrared spectroscopy, gas chromatography/mass spectrometry, and nuclear magnetic resonance spectroscopy analyses revealed that CFPS-1 has a backbone of 1,6-linked and 1,4,6-linked-α-D-Glc, which is terminated with a 1-linked-α-D-Man residue at the O-4 position of 1,4,6-linked-α-D-Glc, in a molar ratio of 3:1:1. Preliminary in vitro bioactivity tests revealed that CFPS-1 effectively and dose-dependently inhibits human breast cancer MCF-7 and MDA-MB-231 cell growth, with an IC50 of 243 ± 6.79 and 1142 ± 14.84 μg/mL, respectively. In MCF-7, CFPS-1 produced a significant up-regulation of p53, p21, Bax and cleaved caspase-7 and down-regulation of Cdk4, cyclin D1, Bcl-2 and caspase-7. These effects resulted in cell cycle blockade at the S-phase and apoptosis induction. In contrast, in MDA-MB-231, with limited degree of change in cell cycle distribution, CFPS-1 increases the proportion of cells in apoptotic sub-G1 phase executed by down-regulation of Bcl-2 and caspase-7 and up-regulation of Bax and cleaved caspase-7. This study extends our understanding of the anticancer mechanism of C. fluminea protein-bound polysaccharide.
authors.affiliation: Department of Nutrition and Food Safety, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, China.
authors.forename: Ningbo
authors.initials: N
authors.lastname: Liao
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Nutrition and Food Safety, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, China.
authors.forename: Ronghua
authors.initials: R
authors.lastname: Zhang
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Xingqian
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Nutrition and Food Safety, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, Zhejiang, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Wang
authors.affiliation: Zhejiang Academy of Science & Technology for Inspection & Quarantine, Hangzhou, Zhejiang, China.
authors.forename: Yuexia
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Shiguo
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou, Zhejiang, China.|Fuli Institute of Food Science, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Donghong
authors.initials: D
authors.lastname: Liu
authors.affiliation: Department of Food Science, Cornell University, Ithaca, New York, United States of America.
authors.forename: Ruihai
authors.initials: R
authors.lastname: Liu
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis; D053179:Caspase 7 / Q000235:genetics / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects*; D049874:Corbicula / Q000737:chemistry*; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D051358:Cyclin-Dependent Kinase 4 / Q000235:genetics / Q000378:metabolism; D005947:Glucose / Q000032:analysis; D006801:Humans; D061986:MCF-7 Cells; D008358:Mannose / Q000032:analysis; D011134:Polysaccharides / Q000737:chemistry / Q000494:pharmacology*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism; D016159:Tumor Suppressor Protein p53 / Q000235:genetics / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D011134:Polysaccharides; D019253:Proto-Oncogene Proteins c-bcl-2; D016159:Tumor Suppressor Protein p53; D019938:Cyclin D1; C495900:CDK4 protein, human; D051358:Cyclin-Dependent Kinase 4; D053179:Caspase 7; D005947:Glucose; D008358:Mannose