title: The Ca2+-activated chloride channel anoctamin-2 mediates spike-frequency adaptation and regulates sensory transmission in thalamocortical neurons.
abstract: Neuronal firing patterns, which are crucial for determining the nature of encoded information, have been widely studied; however, the molecular identity and cellular mechanisms of spike-frequency adaptation are still not fully understood. Here we show that spike-frequency adaptation in thalamocortical (TC) neurons is mediated by the Ca2+-activated Cl-channel (CACC) anoctamin-2 (ANO2). Knockdown of ANO2 in TC neurons results in significantly reduced spike-frequency adaptation along with increased tonic spiking. Moreover, thalamus-specific knockdown of ANO2 increases visceral pain responses. These results indicate that ANO2 contributes to reductions in spike generation in highly activated TC neurons and thereby restricts persistent information transmission.
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Go Eun
authors.initials: GE
authors.lastname: Ha
authors.affiliation: Center for Neural Science, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Jaekwang
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Hankyul
authors.initials: H
authors.lastname: Kwak
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Kiyeong
authors.initials: K
authors.lastname: Song
authors.affiliation: Center for Neural Science, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Jea
authors.initials: J
authors.lastname: Kwon
authors.affiliation: Center for Neural Science, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Soon-Young
authors.initials: SY
authors.lastname: Jung
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Joohyeon
authors.initials: J
authors.lastname: Hong
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Gyeong-Eon
authors.initials: GE
authors.lastname: Chang
authors.affiliation: Center for Functional Connectomics, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Eun Mi
authors.initials: EM
authors.lastname: Hwang
authors.affiliation: Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34141, Republic of Korea.
authors.forename: Hee-Sup
authors.initials: HS
authors.lastname: Shin
authors.affiliation: Center for Neural Science, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: C Justin
authors.initials: CJ
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, College of Life Science and Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.
authors.forename: Eunji
authors.initials: E
authors.lastname: Cheong
mesh_terms: D000256:Adenoviridae; D000818:Animals; D000075368:Anoctamins / Q000235:genetics / Q000378:metabolism*; D000075002:Bestrophins / Q000235:genetics / Q000378:metabolism; D002118:Calcium / Q000494:pharmacology*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D055785:Gene Knockdown Techniques; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D018408:Patch-Clamp Techniques; D011984:Sensory Receptor Cells / Q000502:physiology*; D013788:Thalamus / Q000502:physiology*; D062367:ortho-Aminobenzoates / Q000494:pharmacology
chemical_list: C532161:ANO2 protein, mouse; D000075368:Anoctamins; C491856:Best1 protein, mouse; D000075002:Bestrophins; C585066:N-((4-methoxy)-2-naphthyl)-5-nitroanthranilic acid; D062367:ortho-Aminobenzoates; D002118:Calcium