title: Deep Sequencing of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform.
abstract: The quasispecies variation of hepatitis B virus (HBV) was believed to be a viral response to antiviral treatment and host immune pressure. Sanger sequencing was previously the classic approach for quasispecies analysis, but this method was also time-consuming and laborious. Ultra-deep sequencing has been widely used in viral quasispecies research, especially for low-frequency mutation detection. Here we present a multiple samples deep sequencing method employing the Illumina platform to detect HBV quasispecies variation in patient-derived samples.
authors.affiliation: Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, Department of Infectious Diseases, Institute for Viral Hepatitis, Second Affiliated Hospital of Chongqing Medical University, 1 Medical Road, Yuzhong, Chongqing, China.
authors.forename: Quan-Xin
authors.initials: QX
authors.lastname: Long
authors.affiliation: Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, Department of Infectious Diseases, Institute for Viral Hepatitis, Second Affiliated Hospital of Chongqing Medical University, 1 Medical Road, Yuzhong, Chongqing, China.
authors.forename: Jie-Li
authors.initials: JL
authors.lastname: Hu
authors.affiliation: Key Laboratory of Molecular Biology for Infectious Diseases of Ministry of Education, Department of Infectious Diseases, Institute for Viral Hepatitis, Second Affiliated Hospital of Chongqing Medical University, 1 Medical Road, Yuzhong, Chongqing, China. Ahuang1964@163.com.
authors.forename: Ai-Long
authors.initials: AL
authors.lastname: Huang
mesh_terms: D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D004279:DNA, Viral; D016679:Genome, Viral; D023281:Genomics / Q000379:methods*; D006515:Hepatitis B virus / Q000235:genetics*; D006801:Humans; D016133:Polymerase Chain Reaction; D012984:Software; D064878:Web Browser; D000073336:Whole Genome Sequencing
chemical_list: D004279:DNA, Viral
keywords: Hepatitis B virus; Illumina sequencing