title: Structural elucidation of three antioxidative polysaccharides from Tricholoma lobayense.
abstract: Tricholoma lobayense is a nutritious mushroom with great health benefits. Three polysaccharides with purity higher than 99% were successfully extracted from Tricholoma lobayense. The molecular weights of TLH-1, TLH-2 and TLH-3 were determined to be 8.43×105，5.36×105and 4.53×103Da, respectively. The backbones of TLH-1 and TLH-2 were mainly composed of 1,4-linked α-d-glucopyranosyl. However, polysaccharide TLH-3 was found to be a highly branched glucogalactan, which is made up of 1,3-linked α-d-glucopyranosyl branched at C-6 and 1,3-linked β-d-galactopyranosyl. In vitro antioxidant activity assays revealed that TLH-3 exhibited highest antioxidant activities among the polysaccharides from Tricholoma lobayense, which were comparable to those of ascorbic acid. The results suggested that the outstanding antioxidant activities of TLH-3 might depend on its low molecular weight, high branch degree, versatile linkage types and complex conformation. These characteristics make TLH-3 an attractive natural antioxidant for food and pharmaceutical applications.
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China. Electronic address: chenyan91030@yahoo.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Xue-Hui
authors.initials: XH
authors.lastname: Li
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Li-Yuan
authors.initials: LY
authors.lastname: Zhou
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Wan
authors.initials: W
authors.lastname: Li
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Liu
authors.initials: L
authors.lastname: Liu
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Wang
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Wen-Na
authors.initials: WN
authors.lastname: Zhang
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Sajid
authors.initials: S
authors.lastname: Hussain
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China.
authors.forename: Xiao-He
authors.initials: XH
authors.lastname: Tian
authors.affiliation: School of Life Sciences and Key Laboratory for Ecological Engineering and Biotechnology, Anhui University, Hefei 230601, PR China. Electronic address: ymlu@ahu.edu.cn.
authors.forename: Yong-Ming
authors.initials: YM
authors.lastname: Lu
mesh_terms: D000975:Antioxidants / Q000737:chemistry*; D015394:Molecular Structure; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry*; D055439:Tricholoma / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D011134:Polysaccharides
keywords: Antioxidant activity; Polysaccharides; Structural elucidation; Tricholoma lobayense