title: Method validation for determination of metals in Vitis labrusca L. grapevine leaf extracts by inductively coupled plasma mass spectrometry (ICP-MS).
abstract: Vitis labrusca L. is the main species used for wine and juice production in Brazil. The grapevine leaves can be used both as functional foods and as cheapest sources for the extraction of phenolic compounds. Besides the antioxidant activity, grapevine leaves exhibited significant anti-inflammatory activity. Therefore, the aim of this study was to develop and validate an analytical methodology to determine the metals selenium (96Se), chromium (53Cr), nickel (62Ni), cadmium (111Cd) and lead (206Pb) in 30 samples of grapevine leaf extracts (Vitis labrusca, Bordo cultivar) using inductively coupled plasma mass spectrometry (ICP-MS). To obtain the grapevine leaf extracts the samples were milled, weighed and digested in microwave oven with nitric acid. The method showed linearity, precision, accuracy and limits of quantification and detection acceptable for INMETRO protocol validation of analytical methods. Therefore, the method using ICP-MS was developed and validated to determine metals concentrations in grapevine leaves of Vitis labrusca L. and the proposed method could be applied in routine analytical laboratory.
authors.affiliation: Centro Universitário Metodista - IPA, Rua Cel. Joaquim Pedro Salgado, 80, Bairro Rio Branco, 90420-060 Porto Alegre, RS, Brazil.
authors.forename: Liane V V
authors.initials: LV
authors.lastname: Bokowski
authors.affiliation: Instituto Federal Sul-Rio-Grandense, Av. Copacabana, 100, Bairro Piratini, 93216-120 Sapucaia do Sul, RS, Brazil.
authors.forename: Roberto B
authors.initials: RB
authors.lastname: Sobrinho
authors.affiliation: Centro Universitário Metodista - IPA, Rua Cel. Joaquim Pedro Salgado, 80, Bairro Rio Branco, 90420-060 Porto Alegre, RS, Brazil.
authors.forename: Cristián J V
authors.initials: CJ
authors.lastname: Armijo
authors.affiliation: Centro Universitário Metodista - IPA, Rua Cel. Joaquim Pedro Salgado, 80, Bairro Rio Branco, 90420-060 Porto Alegre, RS, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Dani
authors.affiliation: Departamento de Biofísica, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Av. Bento Gonçalves, 9500, Bairro Agronomia, 91501-970 Porto Alegre, RS, Brazil.|Instituto de Biotecnologia, Universidade de Caxias do Sul, Rua Francisco Getúlio Vargas, 1130, Campus Universitário de Caxias do Sul, 95070-560 Caxias do Sul, RS, Brazil.
authors.forename: João A P
authors.initials: JA
authors.lastname: Henriques
authors.affiliation: Centro Universitário Metodista - IPA, Rua Cel. Joaquim Pedro Salgado, 80, Bairro Rio Branco, 90420-060 Porto Alegre, RS, Brazil.
authors.forename: Cláudia
authors.initials: C
authors.lastname: Funchal
mesh_terms: D001938:Brazil; D002104:Cadmium / Q000032:analysis; D002857:Chromium / Q000032:analysis; D007854:Lead / Q000032:analysis; D013058:Mass Spectrometry / Q000379:methods; D008670:Metals / Q000032:analysis*; D009532:Nickel / Q000032:analysis; D010636:Phenols / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D012643:Selenium / Q000032:analysis; D027843:Vitis / Q000737:chemistry*
chemical_list: D008670:Metals; D010636:Phenols; D010936:Plant Extracts; D002104:Cadmium; D002857:Chromium; D007854:Lead; D009532:Nickel; D012643:Selenium