title: Estrogen modulates exercise endurance along with mitochondrial uncoupling protein 3 downregulation in skeletal muscle of female mice.
abstract: Estrogen is a hormone that regulates physiological processes and its dysregulation may relate to muscle disorders particularly in female, although the mechanism remains to be elucidated. We here show that estrogen deficiency repressed exercise endurance in female mice whereas the administration of estrogen to ovariectomized mice recovered it. Microarray analysis of mouse muscles showed that mitochondrial uncoupling protein 3 (UCP3) is upregulated by ovariectomy and downregulated by estrogen administration. Intriguingly, ectopic expression of constitutively active estrogen receptor α decreased UCP3 level and increased cellular ATP content in differentiated myoblastic C2C12 cells. Overall, the present study suggests that estrogen plays a critical role in the regulation of energy expenditure and exercise endurance in female.
authors.affiliation: Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan; Department of Obstetrics and Gynecology, Juntendo University, School of Medicine, Tokyo, Japan.
authors.forename: Saki
authors.initials: S
authors.lastname: Nagai
authors.affiliation: Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Ikeda
authors.affiliation: Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
authors.forename: Kuniko
authors.initials: K
authors.lastname: Horie-Inoue
authors.affiliation: Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan.
authors.forename: Sachiko
authors.initials: S
authors.lastname: Shiba
authors.affiliation: Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan; Department of Obstetrics and Gynecology, Juntendo University, School of Medicine, Tokyo, Japan.
authors.forename: Saya
authors.initials: S
authors.lastname: Nagasawa
authors.affiliation: Department of Obstetrics and Gynecology, Juntendo University, School of Medicine, Tokyo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Takeda
authors.affiliation: Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan; Department of Functional Biogerontology, Tokyo Metropolitan Institute of Gerontology, Tokyo, Japan. Electronic address: sinoue@tmig.or.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Inoue
mesh_terms: D000818:Animals; D015536:Down-Regulation / Q000187:drug effects / Q000502:physiology*; D004967:Estrogens / Q000378:metabolism* / Q000494:pharmacology; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000187:drug effects / Q000502:physiology*; D010052:Ovariectomy; D010805:Physical Conditioning, Animal; D010807:Physical Endurance / Q000187:drug effects / Q000502:physiology*; D000071247:Uncoupling Protein 3 / Q000378:metabolism*
chemical_list: D004967:Estrogens; D000071247:Uncoupling Protein 3
keywords: Endurance; Estrogen; Mitochondria; Muscle; Ucp3