title: Nanostructured poly (lactic acid) electrospun fiber with high loadings of TiO2nanoparticles: Insights into bactericidal activity and cell viability.
abstract: Researchers have been looking for modifying surfaces of polymeric biomaterials approved by FDA to obtain nanofeatures and bactericidal properties. If modified, it would be very interesting because the antibiotic administration could be reduced and, therefore, the bacterial resistance. Here, we report the electrospinning of poly (lactic acid) (PLA) with high loadings of titanium dioxide nanoparticles (TiO2, 1-5wt%) and their bactericidal properties. TiO2nanoparticles have been recognized for a long time for their antibacterial, low cost and self-cleaning properties. However, their ability to reduce bacteria functions when used in polymers has not been well studied to date. In this context, we aimed here to generate nanostructured PLA electrospun fiber-TiO2nanoparticle composites for further evaluation of their bactericidal activity and cell viability. TEM and SEM micrographs revealed the successful electrospinning of PLA/TiO2and the generation of polymer-TiO2nanostructures. When increasing the TiO2concentration, we observed a proportional increase in the nanoparticle density along the fiber and surface. The nanostructured PLA/TiO2nanofibers showed no mammalian cell toxicity and, most importantly, possessed bactericidal activity with higher TiO2loads. Such results suggest that the present PLA electrospun fiber-TiO2nanoparticle composites should be further studied for a wide range of biomedical applications.
authors.affiliation: Laboratory of Biomedical Nanotechnology (NANOBIO), University of Vale do Paraiba, Sao Jose dos Campos 12224-000, Sao Paulo, Brazil.
authors.forename: T V
authors.initials: TV
authors.lastname: Toniatto
authors.affiliation: Laboratory of Biomedical Nanotechnology (NANOBIO), University of Vale do Paraiba, Sao Jose dos Campos 12224-000, Sao Paulo, Brazil.
authors.forename: B V M
authors.initials: BVM
authors.lastname: Rodrigues
authors.affiliation: Laboratory of Biomedical Nanotechnology (NANOBIO), University of Vale do Paraiba, Sao Jose dos Campos 12224-000, Sao Paulo, Brazil.
authors.forename: T C O
authors.initials: TCO
authors.lastname: Marsi
authors.affiliation: Laboratory of Biomedical Nanotechnology (NANOBIO), University of Vale do Paraiba, Sao Jose dos Campos 12224-000, Sao Paulo, Brazil.
authors.forename: R
authors.initials: R
authors.lastname: Ricci
authors.affiliation: Laboratory of Biomedical Nanotechnology (NANOBIO), University of Vale do Paraiba, Sao Jose dos Campos 12224-000, Sao Paulo, Brazil; Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
authors.forename: F R
authors.initials: FR
authors.lastname: Marciano
authors.affiliation: Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA.
authors.forename: T J
authors.initials: TJ
authors.lastname: Webster
authors.affiliation: Laboratory of Biomedical Nanotechnology (NANOBIO), University of Vale do Paraiba, Sao Jose dos Campos 12224-000, Sao Paulo, Brazil; Biomaterials Innovation Research Center, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA 02139, USA; Department of Chemical Engineering, Northeastern University, Boston, MA 02115, USA. Electronic address: aolobo@pq.cnpq.br.
authors.forename: A O
authors.initials: AO
authors.lastname: Lobo
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D002460:Cell Line; D006801:Humans; D057139:Nanofibers / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D011091:Polyesters / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D013211:Staphylococcus aureus / Q000254:growth & development*; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D011091:Polyesters; C009495:titanium dioxide; C033616:poly(lactide); D014025:Titanium
keywords: Bactericidal effect; Cytotoxicity; Electrospinning; Nanostructures; Poly (lactic acid); Titanium dioxide