title: Valproic acid protects against MPP+-mediated neurotoxicity in SH-SY5Y Cells through autophagy.
abstract: Autophagy is a common physiological activity in cells. Studies show that dysregulation of autophagy is involved in the pathogenesis of Parkinson's disease (PD). As a commonly used anti-epileptic drug, valproic acid (VPA) has shown neuroprotective effects in PD. The aim of this study was to explore whether the autophagy induced by VPA involved in the neuroprotective effects in PD cell model. We found that VPA treatment counteracted MPP+-caused autophagic flux impairment. Forthermore, VPA could alleviates apoptosis, reduce reactive oxygen species (ROS) production and mitochondrial membrane potential (MMP) loss caused by MPP+. And we also observed that VPA up-regulated the active caspase-3 and Bcl-2/Bax ratio and inhibited cytochrome c (Cyt c) release from mitochondria to the cytoplasm. However, 3-Methyladenine (3-MA) or bafilomycin A1 (Baf-A1), blockers for autophagy, partially weakened the neuroprotective effect of VPA. Our findings suggest that the neuroprotective effect of VPA on neuroblastoma cells may partially result from inducing autophagy and related to the inhibition of the mitochondrial apoptosis pathway.
authors.affiliation: Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China; Department of Neurology, Drum Tower Hospital of Nanjing Medical University, Nanjing, Jiangsu, China.
authors.forename: J Y
authors.initials: JY
authors.lastname: Wu
authors.affiliation: Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: L H
authors.initials: LH
authors.lastname: Weng
authors.affiliation: Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: X X
authors.initials: XX
authors.lastname: Li
authors.affiliation: Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Yu
authors.affiliation: Department of Neurology, Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China; Department of Neurology, Drum Tower Hospital of Nanjing Medical University, Nanjing, Jiangsu, China. Electronic address: xuyun20042001@aliyun.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Xu
mesh_terms: D015655:1-Methyl-4-phenylpyridinium / Q000633:toxicity*; D000225:Adenine / Q000031:analogs & derivatives / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D001343:Autophagy / Q000187:drug effects*; D045744:Cell Line, Tumor; D002470:Cell Survival; D006801:Humans; D018942:Macrolides / Q000494:pharmacology; D008928:Mitochondria / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D010300:Parkinson Disease / Q000473:pathology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D014635:Valproic Acid / Q000494:pharmacology*
chemical_list: D018942:Macrolides; D018696:Neuroprotective Agents; D017382:Reactive Oxygen Species; C025946:3-methyladenine; D014635:Valproic Acid; C040929:bafilomycin A1; D000225:Adenine; D015655:1-Methyl-4-phenylpyridinium
keywords: Apoptosis; Autophagy; Mitochondria; Parkinson’s disease; Valproic acid