title: A novel multiplex poliovirus binding inhibition assay applicable for large serosurveillance and vaccine studies, without the use of live poliovirus.
abstract: Large-scale serosurveillance or vaccine studies for poliovirus using the "gold standard" WHO neutralisation test (NT) are very laborious and time consuming. With the polio eradication at hand and with the removal of live attenuated Sabin strains from the oral poliovirus vaccine (OPV), starting with type 2 (as of April 2016), laboratories will need to conform to much more stringent laboratory biosafety regulations when handling live poliovirus strains. In this study, a poliovirus binding inhibition multiplex immunoassay (polio MIA) using inactivated poliovirus vaccine (IPV-Salk) was developed for simultaneous quantification of serum antibodies directed to all three poliovirus types. Our assay shows a good correlation with the NT and an excellent correlation with the ELISA-based binding inhibition assay (POBI). The assay is highly type-specific and reproducible. Additionally, serum sample throughput increases about fivefold relative to NT and POBI and the amount of serum needed is reduced by more than 90%. In conclusion, the polio MIA can be used as a safe and high throughput application, especially for large-scale surveillance and vaccine studies, reducing laboratory time and serum amounts needed.
authors.affiliation: National Institute of Public Health and the Environment (RIVM), Centre for Infectious Disease Control, Bilthoven, The Netherlands. Electronic address: Rutger.Schepp@rivm.nl.
authors.forename: Rutger M
authors.initials: RM
authors.lastname: Schepp
authors.affiliation: National Institute of Public Health and the Environment (RIVM), Centre for Infectious Disease Control, Bilthoven, The Netherlands.
authors.forename: Guy A M
authors.initials: GA
authors.lastname: Berbers
authors.affiliation: Department of Statistics, Informatics and Modelling, Bilthoven, The Netherlands.
authors.forename: José A
authors.initials: JA
authors.lastname: Ferreira
authors.affiliation: National Institute of Public Health and the Environment (RIVM), Centre for Infectious Disease Control, Bilthoven, The Netherlands.
authors.forename: Johan H
authors.initials: JH
authors.lastname: Reimerink
authors.affiliation: National Institute of Public Health and the Environment (RIVM), Centre for Infectious Disease Control, Bilthoven, The Netherlands.
authors.forename: Fiona R
authors.initials: FR
authors.lastname: van der Klis
mesh_terms: D000914:Antibodies, Viral / Q000097:blood*; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007157:Immunologic Surveillance; D009500:Neutralization Tests; D017955:Poliovirus / Q000276:immunology*; D011054:Poliovirus Vaccine, Inactivated / Q000276:immunology*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D038301:Virus Inactivation
chemical_list: D000914:Antibodies, Viral; D011054:Poliovirus Vaccine, Inactivated
keywords: Binding inhibition; Immunoassay; Multiplex; Neutralization test; Polio eradication; Poliovirus