title: Improvement of Heart Failure by Human Amniotic Mesenchymal Stromal Cell Transplantation in Rats.
abstract: Background:Recently, stem cells have been considered for the treatment of heart diseases, but no marked improvement has been recorded. This is the first study to examine the functional and histological effects of the transplantation of human amniotic mesenchymal stromal cells (hAMSCs) in rats with heart failure (HF).Methods:This study was conducted in the years 2014 and 2015. 35 male Wistar rats were randomly assigned into 5 equal experimental groups (7 rats each) as 1- Control 2- Heart Failure (HF) 3- Sham 4- Culture media 5- Stem Cell Transplantation (SCT). Heart failure was induced using 170 mg/kg/d of isoproterenol subcutaneously injection in 4 consecutive days. The failure confirmed by the rat cardiac echocardiography on day 28. In SCT group, 3×106cells in 150 µl of culture media were transplanted to the myocardium. At the end, echocardiographic and hemodynamic parameters together with histological evaluation were done.Results:Echocardiography results showed that cardiac ejection fraction in HF group increased from 58/73 ± 9% to 81/25 ± 6/05% in SCT group (p value < 0.001). Fraction shortening in HF group was increased from 27/53 ± 8/58% into 45/55 ± 6/91% in SCT group (p value < 0.001). Furthermore, hAMSCs therapy significantly improved mean diastolic blood pressure, mean arterial pressure, left ventricular systolic pressure, rate pressure product, and left ventricular end-diastolic pressure compared to those in the HF group, with the values reaching the normal levels in the control group. A marked reduction in fibrosis tissue was also found in the SCT group (p value < 0.001) compared with the animals in the HF group.Conclusion:The transplantation of hAMSCs in rats with heart failure not only decreased the level of fibrosis but also conferred significant improvement in heart performance in terms of echocardiographic and hemodynamic parameters.
authors.affiliation: Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.; Medical Biotechnology Research Center, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Seyed Mohammad Taghi
authors.initials: SM
authors.lastname: Razavi Tousi
authors.affiliation: Department of Physiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Faghihi
authors.affiliation: Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran .
authors.forename: Maliheh
authors.initials: M
authors.lastname: Nobakht
authors.affiliation: Department of Veterinary Diagnostic Imaging, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran .
authors.forename: Mohammad
authors.initials: M
authors.lastname: Molazem
authors.affiliation: Oncopathology Research Center, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Elham
authors.initials: E
authors.lastname: Kalantari
authors.affiliation: Rajaie Cardiovascular, Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran .
authors.forename: Amir
authors.initials: A
authors.lastname: Darbandi Azar
authors.affiliation: Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran .
authors.forename: Nahid
authors.initials: N
authors.lastname: Aboutaleb
keywords: Cell transplantation; Heart failure; Mesenchymal stromal cells