title: Isolation of Platelet-Derived Extracellular Vesicles.
abstract: Platelets participate in several physiological functions, including hemostasis, immunity, and development. Additionally, platelets play key roles in arterial thrombosis and cancer progression. Given this plethora of functions, there is a strong interest of the role of platelet-derived (extracellular) vesicles (PDEVs) as functional mediators and biomarkers. Moreover, the majority of the blood-borne EVs are thought to originate from either platelets or directly from the platelet precursor cells, the megakaryocytes, which reside in the bone marrow. To circumvent confusion, we use the term PDEVs for both platelet-derived and/or megakaryocyte-derived EVs. PDEVs can be isolated from blood or from isolated platelets after activation. In this chapter, we describe all commonly used PDEV isolation methods from blood and prepurified platelets.
authors.affiliation: Division of Biochemistry and Biotechnology, Faculty of Biological and Environmental Sciences, University of Helsinki, Viikinkaari 5 D, 56, 00014, Helsinki, Finland.
authors.forename: Maria
authors.initials: M
authors.lastname: Aatonen
authors.affiliation: Division of Biochemistry and Biotechnology, Faculty of Biological and Environmental Sciences, University of Helsinki, Viikinkaari 5 D, 56, 00014, Helsinki, Finland.|Laboratory of Experimental Clinical Chemistry, Vesicle Observation Center, Academic Medical Centre of the University ofAmsterdam, Amsterdam, The Netherlands.
authors.forename: Sami
authors.initials: S
authors.lastname: Valkonen
authors.affiliation: Laboratory of Experimental Clinical Chemistry, Vesicle Observation Center, Academic Medical Centre of the University ofAmsterdam, Amsterdam, The Netherlands.
authors.forename: Anita
authors.initials: A
authors.lastname: Böing
authors.affiliation: Laboratory of Experimental Clinical Chemistry, Vesicle Observation Center, Academic Medical Centre of the University ofAmsterdam, Amsterdam, The Netherlands.
authors.forename: Yuana
authors.initials: Y
authors.lastname: Yuana
authors.affiliation: Laboratory of Experimental Clinical Chemistry, Vesicle Observation Center, Academic Medical Centre of the University ofAmsterdam, Amsterdam, The Netherlands.
authors.forename: Rienk
authors.initials: R
authors.lastname: Nieuwland
authors.affiliation: Division of Biochemistry and Biotechnology, Faculty of Biological and Environmental Sciences, University of Helsinki, Viikinkaari 5 D, 56, 00014, Helsinki, Finland. pia.siljander@helsinki.fi.
authors.forename: Pia
authors.initials: P
authors.lastname: Siljander
mesh_terms: D001792:Blood Platelets / Q000378:metabolism*; D002458:Cell Fractionation / Q000379:methods; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D002499:Centrifugation, Density Gradient / Q000379:methods; D002850:Chromatography, Gel / Q000379:methods; D000067128:Extracellular Vesicles / Q000378:metabolism*; D006801:Humans; D010949:Plasma / Q000378:metabolism; D015539:Platelet Activation
keywords: Exosome; Extracellular vesicle; Gradient centrifugation; Immunobeads; Isolation; Microparticle; Platelet; Size-exclusion chromatography