title: Ligand-responsive upregulation of 3' CITE-mediated translation in a wheat germ cell-free expression system.
abstract: We have rationally constructed a novel regulation-type of artificial riboswitch that ligand-dose dependently upregulates translation initiation mediated by a 3' cap-independent translation element (3' CITE) with no major hybridization switches in a plant expression system (wheat germ extract).
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. ogawa.atsushi.mf@ehime-u.ac.jp.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Ogawa
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. ogawa.atsushi.mf@ehime-u.ac.jp.
authors.forename: Yuta
authors.initials: Y
authors.lastname: Murashige
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. ogawa.atsushi.mf@ehime-u.ac.jp.
authors.forename: Junichiro
authors.initials: J
authors.lastname: Tabuchi
authors.affiliation: Proteo-Science Center, Ehime University, 3 Bunkyo-cho, Matsuyama, Ehime 790-8577, Japan. ogawa.atsushi.mf@ehime-u.ac.jp.
authors.forename: Taiki
authors.initials: T
authors.lastname: Omatsu
mesh_terms: D020413:3' Untranslated Regions; D002474:Cell-Free System; D015870:Gene Expression; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017930:Genes, Reporter; D005854:Germ Cells / Q000378:metabolism*; D008024:Ligands; D014176:Protein Biosynthesis; D058928:Riboswitch; D014908:Triticum / Q000235:genetics* / Q000378:metabolism*
chemical_list: D020413:3' Untranslated Regions; D008024:Ligands; D058928:Riboswitch