title: Effects of RFamide-related peptide-1 (RFRP-1) microinjections into the central nucleus of amygdala on passive avoidance learning in rats.
abstract: The amygdaloid body (AMY) plays an important role in memory, learning and reward-related processes. RFRP-1 immunoreactive fibers and NPFF receptors were identified in the AMY, and previously we verified that RFRP-1 infused into the central nucleus of AMY (CeA) induced place preference. The aim of the present study was to examine the possible effects of RFRP-1 in the CeA on passive avoidance learning. Male Wistar rats were examined in two-compartment passive avoidance paradigm. Animals were shocked with 0.5mA current and subsequently were microinjected bilaterally with 50ng or 100ng RFRP-1 in volume of 0.4μl, or 20ng NPFF receptor antagonist RF9 (ANT) alone, or antagonist 15min before 50ng RFRP-1 treatments into the CeA. Fifty nanogram dose of RFRP-1 significantly increased the step-through latency time, the 100ng RFRP-1 and the ANT alone were ineffective. The effect of 50ng RFRP-1 was eliminated by the ANT pretreatment. Our results suggest that intraamygdaloid RFRP-1 enhances learning processes and memory in aversive situations and this effect can specifically be prevented by ANT pretreatment.
authors.affiliation: Institute of Physiology, Pécs University Medical School, Hungary.
authors.forename: Anita
authors.initials: A
authors.lastname: Kovács
authors.affiliation: Institute of Physiology, Pécs University Medical School, Hungary.
authors.forename: Kristóf
authors.initials: K
authors.lastname: László
authors.affiliation: Institute of Physiology, Pécs University Medical School, Hungary.
authors.forename: Olga
authors.initials: O
authors.lastname: Zagoracz
authors.affiliation: Institute of Physiology, Pécs University Medical School, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Ollmann
authors.affiliation: Institute of Physiology, Pécs University Medical School, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Péczely
authors.affiliation: Institute of Physiology, Pécs University Medical School, Hungary.
authors.forename: Rita
authors.initials: R
authors.lastname: Gálosi
authors.affiliation: Institute of Physiology, Pécs University Medical School, Hungary; Molecular Neurophysiology Research Group, Pécs University, Szentágothai Research Center, Pécs, Hungary. Electronic address: Laszlo.Lenard@aok.pte.hu.
authors.forename: László
authors.initials: L
authors.lastname: Lénárd
mesh_terms: D000818:Animals; D001362:Avoidance Learning / Q000187:drug effects*; D001522:Behavior, Animal / Q000187:drug effects; D066274:Central Amygdaloid Nucleus / Q000187:drug effects*; D008297:Male; D008568:Memory / Q000187:drug effects*; D008845:Microinjections / Q000379:methods; D009479:Neuropeptides / Q000378:metabolism / Q000494:pharmacology*; D017208:Rats, Wistar; D018013:Receptors, Neuropeptide / Q000187:drug effects
chemical_list: D009479:Neuropeptides; C416987:RFamide peptide; D018013:Receptors, Neuropeptide; C083929:neuropeptide FF receptor