title: Modified Carba NP test for the detection of carbapenemase production in gram-negative rods: optimized handling of multiple samples.
abstract: The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria. No false-positive results were obtained, but false-negative results were observed with OXA-23- and GES-carbapenemase-producing isolates. Aeromonas sp. are not testable by Modified Carba NP.
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil. Electronic address: elocampana@gmail.com.
authors.forename: Eloiza H
authors.initials: EH
authors.lastname: Campana
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil.
authors.forename: Stephanie G
authors.initials: SG
authors.lastname: Chuster
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil.
authors.forename: Isadora R
authors.initials: IR
authors.lastname: da Silva
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil.
authors.forename: Raphael P
authors.initials: RP
authors.lastname: Paschoal
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil.
authors.forename: Raquel R
authors.initials: RR
authors.lastname: Bonelli
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil.
authors.forename: Beatriz M
authors.initials: BM
authors.lastname: Moreira
authors.affiliation: Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Laboratório de Investigação em Microbiologia Médica, Rio de Janeiro, Brazil.
authors.forename: Renata C
authors.initials: RC
authors.lastname: Picão
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis*; D001431:Bacteriological Techniques / Q000379:methods*; D005188:False Negative Reactions; D006090:Gram-Negative Bacteria / Q000201:enzymology*; D001618:beta-Lactamases / Q000032:analysis*
chemical_list: D001426:Bacterial Proteins; D001618:beta-Lactamases; C063912:carbapenemase
keywords: Carbapenemases; Diagnostics; Gram-negative bacteria