title: Comparative Analysis of Proteome Patterns of Francisella tularensis Isolates from Patients and the Environment.
abstract: Francisella tularensis is the causative agent of tularemia. Although major contributors and the main mechanism of the virulence are well known, some of the molecular details are still missing. Proteomics studies regarding F. tularensis have provided snapshot pictures of the organism grown under different culture conditions to understand the mechanism of virulence. In general, such studies were carried out with standard strains e.g., LVS and did not involve comparisons of F. tularensis isolates from either clinical or environmental sources. In this study, we performed two-dimensional gel electrophoresis (2DE)-based proteomic analysis and compared the protein profiles of the F. tularensis subsp. holarctica strains isolated from the clinical and the environmental samples. Regulations were detected in 14 spots when twofold regulation criteria were applied. The regulated protein spots were subjected to MALDI-TOF/TOF analysis and identified. Classification of the identified proteins based on metabolic functions revealed that the translation machinery was the most varying metabolic processes among the isolates. Using normalized protein spot intensities, PCA analysis was also performed. The results indicated that the strain isolated from water source was different then the strains isolated from the patients. Most interestingly, the isolates were strikingly distinguishable from the standard NCTC 10857 strain.
authors.affiliation: Medical Biology Department/DEKART Proteomics Laboratory, Kocaeli University Medical Faculty, Umuttepe, Kocaeli, 41380, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Kasap
authors.affiliation: Department of Medical Microbiology, Kocaeli University Medical Faculty, Umuttepe, Kocaeli, 41380, Turkey. aynuryk2010@yahoo.com.
authors.forename: Aynur
authors.initials: A
authors.lastname: Karadenizli
authors.affiliation: Medical Biology Department/DEKART Proteomics Laboratory, Kocaeli University Medical Faculty, Umuttepe, Kocaeli, 41380, Turkey.
authors.forename: Gürler
authors.initials: G
authors.lastname: Akpınar
authors.affiliation: Department of Medical Microbiology, Kocaeli University Medical Faculty, Umuttepe, Kocaeli, 41380, Turkey.
authors.forename: Hüseyin
authors.initials: H
authors.lastname: Uzuner
authors.affiliation: Department of Biomedical Engineering, Kocaeli University Technology Faculty, Kocaeli, Turkey.
authors.forename: Abula
authors.initials: A
authors.lastname: Ayimugu
authors.affiliation: Department of Biomedical Engineering, Kocaeli University Technology Faculty, Kocaeli, Turkey.
authors.forename: Kübra
authors.initials: K
authors.lastname: Karaosmanoğlu
authors.affiliation: Department of Medical Microbiology, Kocaeli University Medical Faculty, Umuttepe, Kocaeli, 41380, Turkey.
authors.forename: Doğanhan Kadir
authors.initials: DK
authors.lastname: Er
mesh_terms: D015180:Electrophoresis, Gel, Two-Dimensional; D004783:Environmental Microbiology; D005604:Francisella tularensis / Q000737:chemistry* / Q000302:isolation & purification*; D006801:Humans; D020543:Proteome / Q000032:analysis*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014406:Tularemia / Q000382:microbiology*
chemical_list: D020543:Proteome