title: CD14++CD16-monocytes are the main source of 11β-HSD type 1 after IL-4 stimulation.
abstract: The anti-inflammatory actions of IL-4 are well established through earlier findings. However, the exact mechanism it uses to downregulate the pro-inflammatory cytokine production through monocytes and macrophages is poorly understood. In this study, we examined the effect of IL-4 in the induction of 11β-HSD1 in the two main classes of monocytes, CD14++CD16-(CD14) and CD14+CD16+(CD16). Peripheral Blood Mononuclear Cells (PBMCs) were isolated from 17 healthy donors and were sorted into CD14 and CD16 subpopulations using cell sorting. Effect of IL-4 on 11β-HSD1-enzyme activity was measured in sorted and unsorted monocytes using Homogeneous Time-Resolved Fluorescence (HTRF) and M1/M2 polarization analysis was performed by flow cytometry. Our results indicate that CD14 cells are the major source of 11β-HSD1 enzyme after IL-4 stimulation and that M2 phenotype is not a pre-requisite for its synthesis.
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: vid1488@gmail.com.
authors.forename: Vidya
authors.initials: V
authors.lastname: Kunnathully
authors.affiliation: Department of Neurological, Biomedical and Movement Sciences, Section of Biology and Genetics, University of Verona, VR 37134, Italy. Electronic address: macarena.gomezlira@univr.it.
authors.forename: Macarena
authors.initials: M
authors.lastname: Gomez-Lira
authors.affiliation: Department of Medicine, Section of Hematology, University of Verona, VR 37134, Italy. Electronic address: giulio.bassi@univr.it.
authors.forename: Giulio
authors.initials: G
authors.lastname: Bassi
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: fabio.poli@univr.it.
authors.forename: Fabio
authors.initials: F
authors.lastname: Poli
authors.affiliation: Applied Research on Cancer Network, University of Verona, VR 37134, Italy. Electronic address: elisa.zoratti@gmail.com.
authors.forename: Elisa
authors.initials: E
authors.lastname: Zoratti
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: valentina.laverde@gmail.com.
authors.forename: Valentina
authors.initials: V
authors.lastname: La Verde
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: luca.idolazzi@univr.it.
authors.forename: Luca
authors.initials: L
authors.lastname: Idolazzi
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: davide.gatti@univr.it.
authors.forename: Davide
authors.initials: D
authors.lastname: Gatti
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: ombretta.viapiana@univr.it.
authors.forename: Ombretta
authors.initials: O
authors.lastname: Viapiana
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: silvano.adami@univr.it.
authors.forename: Silvano
authors.initials: S
authors.lastname: Adami
authors.affiliation: Department of Medicine, Section of Rheumatology, University of Verona, VR 37134, Italy. Electronic address: maurizio.rossini@univr.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Rossini
mesh_terms: D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1 / Q000235:genetics / Q000378:metabolism*; D002454:Cell Differentiation; D002469:Cell Separation; D002478:Cells, Cultured; D004789:Enzyme Activation; D005434:Flow Cytometry; D005786:Gene Expression Regulation; D006801:Humans; D007114:Immunization; D015847:Interleukin-4 / Q000276:immunology; D018950:Lipopolysaccharide Receptors / Q000378:metabolism; D008264:Macrophages / Q000378:metabolism*; D009000:Monocytes / Q000378:metabolism*; D010641:Phenotype; D017452:Receptors, IgG / Q000378:metabolism
chemical_list: D018950:Lipopolysaccharide Receptors; D017452:Receptors, IgG; D015847:Interleukin-4; D043205:11-beta-Hydroxysteroid Dehydrogenase Type 1
keywords: 11β-HSD1; Anti-inflammatory; Glucocorticoids; IL-4; M2; Monocytes