title: Comparative Analysis of Ultrafine Granular Powder and Decoction Pieces of Salvia miltiorrhiza by UPLC-UV-MSn Combined with Statistical Analysis.
abstract: Salvia miltiorrhizais one of the most used herbal medicines for the treatment of a wide range of diseases in China. Decoction pieces and Chinese patent medicines fromS. miltiorrhizaare the two main types of products used by patients. Other relatively new products ofS. miltiorrhiza, like injections and ultrafine granular powder (D90< 45 µm before granulation), are also increasingly used nowadays. With the growing usage of new products ofS. miltiorrhiza, their chemical components and pharmacological effects, compared to the traditional decoction pieces, are attracting attention. In this work, the chemical profiles of two types of products fromS. miltiorrhiza(one is traditional "decoction pieces", the other is modern "ultrafine granular powder") were compared via similarity analysis and discriminated via multivariate analysis, e.g., hierarchical cluster analysis, principal component analysis, and partial least squares discrimination analysis. A new adamantane stationary phase column was used to establish informative chemical profiles of them. The mean similarity correlation coefficient (> 0.987) revealed that ultrafine granular powder and decoction pieces ofS. miltiorrhizawere consistent between each other and stable between different batches. Two types ofS. miltiorrhizaproducts were clearly resolved from each other by hierarchical cluster analysis, principal component analysis, and partial least squares discrimination analysis. Compounds responsible for the discrimination results were further characterized by ESI-MS/MS. Eventually, 62 compounds selected as characteristic markers for evaluation were characterized or tentatively characterized. This result will facilitate the further comparison of these two types ofS. miltiorrhizaproducts in pharmacology and pharmacokinetics.
authors.affiliation: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Shen
authors.affiliation: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Jinjun
authors.initials: J
authors.lastname: Hou
authors.affiliation: The Key Laboratory of Technology of Breaking Cell Wall and Application in Chinese Medicine Decoction Pieces, Guangzhou, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Deng
authors.affiliation: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Zijin
authors.initials: Z
authors.lastname: Feng
authors.affiliation: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Min
authors.initials: M
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Technology of Breaking Cell Wall and Application in Chinese Medicine Decoction Pieces, Guangzhou, China.
authors.forename: Jinle
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: Wanying
authors.initials: W
authors.lastname: Wu
authors.affiliation: Shanghai Research Center for Modernization of Traditional Chinese Medicine, National Engineering Laboratory for TCM Standardization Technology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China.
authors.forename: De-An
authors.initials: DA
authors.lastname: Guo
mesh_terms: D004365:Drugs, Chinese Herbal / Q000737:chemistry* / Q000302:isolation & purification; D016018:Least-Squares Analysis; D011208:Powders; D025341:Principal Component Analysis; D027561:Salvia miltiorrhiza / Q000737:chemistry*; D053719:Tandem Mass Spectrometry
chemical_list: D004365:Drugs, Chinese Herbal; D011208:Powders