title: Selective ex vivo photothermal nano-therapy of solid liver tumors mediated by albumin conjugated gold nanoparticles.
abstract: We have used albumin (BSA) bound to gold nanoparticles (GNPs) as active vectors to target liver cells. Our incentive to develop an original model of living liver cancer sprang from the ethical drawbacks that hindered the assessment of the selective character and the therapeutic capacity of these nano-biosystems in cancer patients. Ex vivo-perfused liver specimens were obtained from hepatocellular carcinoma patients similarly to the surgical technique of transplantation. Albumin bound to GNPs was inoculated intra-arterially onto the resulting specimen and determined the specific delivery of the nano-bioconjugate into the malignant tissue by means of the capillary bed. The extent of necrosis was considerable following laser therapy and at the same time surrounding parenchyma was not seriously affected. The selective photothermal ablation of the malignant liver tissue was obtained after the selective accumulation of BSA bound to GNPs into tumor cells following ex-vivo intra-vascular perfusion.
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania; Department of Surgery, "Iuliu Hatieganu" University of Medicine and Pharmacy, Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Lucian
authors.initials: L
authors.lastname: Mocan
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.
authors.forename: Cristian
authors.initials: C
authors.lastname: Matea
authors.affiliation: Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine, 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors.forename: Flaviu A
authors.initials: FA
authors.lastname: Tabaran
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania; Department of Gastroenterology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Mosteanu
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania; Department of Gastroenterology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Teodora
authors.initials: T
authors.lastname: Pop
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania; Department of Surgery, "Iuliu Hatieganu" University of Medicine and Pharmacy, Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Cosmin
authors.initials: C
authors.lastname: Puia
authors.affiliation: Department of Internal Medicine, "Iuliu Hatieganu" University of Medicine and Pharmacy, Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Lucia
authors.initials: L
authors.lastname: Agoston-Coldea
authors.affiliation: Department of Neonatology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zaharie
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania; Department of Physiology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Teodora
authors.initials: T
authors.lastname: Mocan
authors.affiliation: Department of Clinical Pharmacology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Victor Babes 8, Cluj-Napoca, Romania.
authors.forename: Anca Dana
authors.initials: AD
authors.lastname: Buzoianu
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania; Department of Surgery, "Iuliu Hatieganu" University of Medicine and Pharmacy, Croitorilor 19-21, Cluj-Napoca, Romania. Electronic address: cornel.iancu@umfcluj.ro.
authors.forename: Cornel
authors.initials: C
authors.lastname: Iancu
mesh_terms: D006046:Gold / Q000627:therapeutic use*; D056945:Hep G2 Cells; D006801:Humans; D006979:Hyperthermia, Induced / Q000379:methods*; D008113:Liver Neoplasms / Q000473:pathology / Q000628:therapy*; D053768:Metal Nanoparticles / Q000627:therapeutic use*; D053769:Nanocapsules / Q000008:administration & dosage / Q000737:chemistry; D058726:Nanoconjugates / Q000627:therapeutic use; D010789:Phototherapy / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000008:administration & dosage* / Q000737:chemistry; D016896:Treatment Outcome; D014407:Tumor Cells, Cultured
chemical_list: D053769:Nanocapsules; D058726:Nanoconjugates; D012710:Serum Albumin, Bovine; D006046:Gold
keywords: Albumin; Ex vivo experiment; Gold nanoparticles; Laser; Liver cancer