title: Chemopreventive Potential of Powdered Red Wine Pomace Seasonings against Colorectal Cancer in HT-29 Cells.
abstract: This study evaluates the antiproliferative and antigenotoxic actions of powdered red wine pomace seasonings (Sk-S, seedless; W-S, whole; Sd-S, seeds). In vitro gastrointestinal digested and colonic fermented fractions of the seasonings were used as cell treatments. Phenolic acids from Sk-S showed the highest bioaccessibility in the small intestine, whereas polyphenols contained in Sd-S might be the most fermentable in the colon. Dietary fiber from Sk-S was the best substrate for short chain fatty acids production by gut microbiota. Colon cancerous (HT-29) cell viability was inhibited by 50% (IC50values) at treatment concentrations ranging from 845 (Sk-S) to 1085 (Sd-S) μg/mL prior digestion, but all digested fractions exhibited similar antiproliferative activities (mean IC50= 814 μg/mL). Oxidative DNA damage in cells was also attenuated by the treatments (200 μg/mL, 24 h preincubation), with all colonic fermented fractions displaying similar genoprotective action. These results suggest the potential of red wine pomace seasonings as chemopreventive agents in colorectal cancer.
authors.affiliation: Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos , Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: Raquel
authors.identifier: http://orcid.org/0000-0002-9224-3872
authors.initials: R
authors.lastname: Del Pino-García
authors.affiliation: Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos , Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: María D
authors.initials: MD
authors.lastname: Rivero-Pérez
authors.affiliation: Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos , Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: María L
authors.initials: ML
authors.lastname: González-SanJosé
authors.affiliation: Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos , Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: Miriam
authors.initials: M
authors.lastname: Ortega-Heras
authors.affiliation: Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos , Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: Javier
authors.initials: J
authors.lastname: García Lomillo
authors.affiliation: Department of Food Biotechnology and Science, Faculty of Sciences, University of Burgos , Plaza Misael Bañuelos s/n, 09001, Burgos, Spain.
authors.forename: Pilar
authors.identifier: http://orcid.org/0000-0002-9306-0082
authors.initials: P
authors.lastname: Muñiz
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000503:physiopathology / Q000517:prevention & control*; D004043:Dietary Fiber / Q000378:metabolism; D004063:Digestion; D000069196:Gastrointestinal Microbiome / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007421:Intestine, Small / Q000378:metabolism / Q000382:microbiology; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D020011:Protective Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D027843:Vitis / Q000737:chemistry*; D014866:Waste Products / Q000032:analysis*; D014920:Wine / Q000032:analysis*
chemical_list: D004043:Dietary Fiber; D010936:Plant Extracts; D020011:Protective Agents; D014866:Waste Products
keywords: antiproliferative activity; chemoprevention; colon cancer; genoprotection; oxidative stress; wine pomace