title: IDC2 and IDC3, two genes involved in cell non-autonomous signaling of fruiting body development in the model fungus Podospora anserina.
abstract: Filamentous ascomycetes produce complex multicellular structures during sexual reproduction. Little is known about the genetic pathways enabling the construction of such structures. Here, with a combination of classical and reverse genetic methods, as well as genetic mosaic and graft analyses, we identify and provide evidence for key roles for two genes during the formation of perithecia, the sexual fruiting bodies, of the filamentous fungus Podospora anserina. Data indicate that the proteins coded by these two genes function cell-non-autonomously and that their activity depends upon conserved cysteines, making them good candidate for being involved in the transmission of a reactive oxygen species (ROS) signal generated by the PaNox1 NADPH oxidase inside the maturing fruiting body towards the PaMpk1 MAP kinase, which is located inside the underlying mycelium, in which nutrients are stored. These data provide important new insights to our understanding of how fungi build multicellular structures.
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France.
authors.forename: Hervé
authors.initials: H
authors.lastname: Lalucque
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France.
authors.forename: Fabienne
authors.initials: F
authors.lastname: Malagnac
authors.affiliation: Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand.
authors.forename: Kimberly
authors.initials: K
authors.lastname: Green
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Gautier
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France.
authors.forename: Pierre
authors.initials: P
authors.lastname: Grognet
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France.
authors.forename: Laetitia
authors.initials: L
authors.lastname: Chan Ho Tong
authors.affiliation: Institute of Fundamental Sciences, Massey University, Palmerston North 4442, New Zealand.
authors.forename: Barry
authors.initials: B
authors.lastname: Scott
authors.affiliation: Univ. Paris Diderot, Sorbonne Paris Cité, Laboratoire Interdisciplinaire des Energies de Demain (LIED), 75205 Paris, France. Electronic address: philippe.silar@univ-paris-diderot.fr.
authors.forename: Philippe
authors.initials: P
authors.lastname: Silar
mesh_terms: D000595:Amino Acid Sequence; D015153:Blotting, Western; D002482:Cellulose / Q000494:pharmacology; D017124:Conserved Sequence; D003545:Cysteine / Q000378:metabolism; D019143:Evolution, Molecular; D048690:Fruiting Bodies, Fungal / Q000235:genetics* / Q000254:growth & development*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D005800:Genes, Fungal; D005816:Genetic Complementation Test; D049452:Green Fluorescent Proteins / Q000378:metabolism; D009030:Mosaicism; D025282:Mycelium / Q000378:metabolism; D010641:Phenotype; D010766:Phosphorylation / Q000187:drug effects; D048468:Podospora / Q000235:genetics* / Q000254:growth & development*; D015398:Signal Transduction / Q000235:genetics*; D013347:Subcellular Fractions / Q000378:metabolism; D014617:Vacuoles / Q000378:metabolism
chemical_list: D005656:Fungal Proteins; D049452:Green Fluorescent Proteins; D002482:Cellulose; D003545:Cysteine
keywords: Developmental mutants; Fungal development; Multicellular fruiting bodies; Perithecium; Podospora anserina