title: Interaction of plant essential oil terpenoids with the southern cattle tick tyramine receptor: A potential biopesticide target.
abstract: An outbreak of the southern cattle tick, Rhipicephalus (Boophilus) microplus, (Canestrini), in the United States would have devastating consequences on the cattle industry. Tick populations have developed resistance to current acaricides, highlighting the need to identify new biochemical targets along with new chemistry. Furthermore, acaricide resistance could further hamper control of tick populations during an outbreak. Botanically-based compounds may provide a safe alternative for efficacious control of the southern cattle tick. We have developed a heterologous expression system that stably expresses the cattle tick's tyramine receptor with a G-protein chimera, producing a system that is amenable to high-throughput screening. Screening an in-house terpenoid library, at two screening concentrations (10 μM and 100 μM), has identified four terpenoids (piperonyl alcohol, 1,4-cineole, carvacrol and isoeugenol) that we believe are positive modulators of the southern cattle tick's tyramine receptor.
authors.affiliation: Pesticide Toxicology Laboratory, Department of Entomology, Iowa State University of Science and Technology, Ames, IA, 50011, USA; Department of Biomedical Science, Iowa State University of Science and Technology, Ames, IA, 50011, USA.
authors.forename: Aaron D
authors.initials: AD
authors.lastname: Gross
authors.affiliation: Knipling-Bushland United States Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, United States Department of Agriculture-Agricultural Research Service, Kerrville, TX, 78028, USA.
authors.forename: Kevin B
authors.initials: KB
authors.lastname: Temeyer
authors.affiliation: Department of Biomedical Science, Iowa State University of Science and Technology, Ames, IA, 50011, USA.
authors.forename: Tim A
authors.initials: TA
authors.lastname: Day
authors.affiliation: Knipling-Bushland United States Livestock Insects Research Laboratory and Veterinary Pest Genomics Center, United States Department of Agriculture-Agricultural Research Service, Kerrville, TX, 78028, USA.
authors.forename: Adalberto A
authors.initials: AA
authors.lastname: Pérez de León
authors.affiliation: Department of Biomedical Science, Iowa State University of Science and Technology, Ames, IA, 50011, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Kimber
authors.affiliation: Pesticide Toxicology Laboratory, Department of Entomology, Iowa State University of Science and Technology, Ames, IA, 50011, USA. Electronic address: jcoats@iastate.edu.
authors.forename: Joel R
authors.initials: JR
authors.lastname: Coats
mesh_terms: D056810:Acaricides / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D000818:Animals; D016466:CHO Cells; D002417:Cattle; D006224:Cricetinae; D003412:Cricetulus; D009822:Oils, Volatile / Q000737:chemistry*; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D011485:Protein Binding; D017982:Receptors, Biogenic Amine / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D013729:Terpenes / Q000737:chemistry / Q000378:metabolism* / Q000633:toxicity; D013987:Ticks / Q000187:drug effects / Q000201:enzymology*
chemical_list: D056810:Acaricides; D009822:Oils, Volatile; D017982:Receptors, Biogenic Amine; D013729:Terpenes; C066175:tyramine receptor
keywords: Essential oils; Monoterpenoid; Rhipicephalus microplus; Southern cattle tick; Tyramine; Tyramine receptor