title: SEC-SANS: size exclusion chromatography combinedin situwith small-angle neutron scattering.
abstract: The first implementation and use of anin situsize exclusion chromatography (SEC) system on a small-angle neutron scattering instrument (SANS) is described. The possibility of deploying such a system for biological solution scattering at the Institut Laue-Langevin (ILL) has arisen from the fact that current day SANS instruments at ILL now allow datasets to be acquired using small sample volumes with exposure times that are often shorter than a minute. This capability is of particular importance for the study of unstable biological macromolecules where aggregation or denaturation issues are a major problem. The first use of SEC-SANS on ILL's instrument D22 is described for a variety of proteins including one particularly aggregation-prone system.
authors.affiliation: Institut Laue-Langevin, 71 avenue des Martyrs, 38042 Grenoble, France; Faculty of Natural Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK.
authors.forename: Ashley
authors.initials: A
authors.lastname: Jordan
authors.affiliation: Institut Laue-Langevin , 71 avenue des Martyrs, 38042 Grenoble, France.
authors.forename: Mark
authors.initials: M
authors.lastname: Jacques
authors.affiliation: Faculty of Natural Sciences, Keele University , Keele, Staffordshire ST5 5BG, UK.
authors.forename: Catherine
authors.initials: C
authors.lastname: Merrick
authors.affiliation: Institut Laue-Langevin , 71 avenue des Martyrs, 38042 Grenoble, France.
authors.forename: Juliette
authors.initials: J
authors.lastname: Devos
authors.affiliation: Institut Laue-Langevin, 71 avenue des Martyrs, 38042 Grenoble, France; Faculty of Natural Sciences, Keele University, Keele, Staffordshire ST5 5BG, UK.
authors.forename: V Trevor
authors.initials: VT
authors.lastname: Forsyth
authors.affiliation: Institut Laue-Langevin , 71 avenue des Martyrs, 38042 Grenoble, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Porcar
authors.affiliation: Institut Laue-Langevin , 71 avenue des Martyrs, 38042 Grenoble, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Martel
keywords: SEC-SANS; in situ; sample environment; size exclusion chromatography; small-angle neutron scattering