title: Valorization of crude glycerol and eggshell biowaste as media components for hydrogen production: A scale-up study using co-culture system.
abstract: The properties of eggshells (EGS) as neutralizing and immobilizing agent were investigated for hydrogen (H2) production using crude glycerol (CG) by co-culture system. Eggshells of different sizes and concentrations were used during batch and repeated-batch fermentation. For batch and repeated-batch fermentation, the maximum H2production (36.53±0.53 and 41.16±0.95mmol/L, respectively) was obtained with the EGS size of 33μm<x5<75μm. Hydrogen production increased with the decreasing size of EGS. Eggshells maintained the fermentation pH (6.00-6.30) and provided immobilization support as confirmed by scanning electron microscopy. As media components, the EGS concentration of 0.25% (w/v) was found to be optimum for maximum H2production (31.66±0.55mmol/L) and the production profile was comparable to H2production (32.07±0.92mmol/L) obtained with all media components. In scale-up study with semi-continuous bioreactor (7.5L), almost 1.5-fold increase (in comparison to mono-culture) i.e. 312.12mmol-H2/L-of medium with 86.65% glycerol utilization was obtained.
authors.affiliation: Institut national de la recherche scientifique, Centre - Eau Terre Environnement, 490, Rue de la Couronne, Québec (QC) G1K 9A9, Canada.
authors.forename: Vinayak Laxman
authors.initials: VL
authors.lastname: Pachapur
authors.affiliation: Institut national de la recherche scientifique, Centre - Eau Terre Environnement, 490, Rue de la Couronne, Québec (QC) G1K 9A9, Canada.
authors.forename: Ratul Kumar
authors.initials: RK
authors.lastname: Das
authors.affiliation: Institut national de la recherche scientifique, Centre - Eau Terre Environnement, 490, Rue de la Couronne, Québec (QC) G1K 9A9, Canada. Electronic address: satinder.brar@ete.inrs.ca.
authors.forename: Satinder Kaur
authors.initials: SK
authors.lastname: Brar
authors.affiliation: Centre de recherche industrielle du Québec (CRIQ), Québec (QC), Canada.
authors.forename: Yann
authors.initials: Y
authors.lastname: Le Bihan
authors.affiliation: Centre de recherche industrielle du Québec (CRIQ), Québec (QC), Canada.
authors.forename: Gerardo
authors.initials: G
authors.lastname: Buelna
mesh_terms: D000818:Animals; D061249:Batch Cell Culture Techniques; D056804:Biofuels; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D018920:Coculture Techniques; D003470:Culture Media / Q000737:chemistry*; D004528:Egg Shell / Q000737:chemistry*; D005285:Fermentation; D005990:Glycerol / Q000737:chemistry*; D006859:Hydrogen / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D008855:Microscopy, Electron, Scanning; D014866:Waste Products
chemical_list: D056804:Biofuels; D003470:Culture Media; D014866:Waste Products; D006859:Hydrogen; D005990:Glycerol
keywords: Co-culture; Crude glycerol; Eggshell; Hydrogen; Immobilization; Neutralization