title: Parthenolide Modulates Immune Response in Cells from C57BL/6 Mice Induced with Experimental Autoimmune Encephalomyelitis.
abstract: Multiple sclerosis is a chronic inflammatory and autoimmune disease of the central nervous system that affects more than 2.5 million people worldwide. Experimental autoimmune encephalomyelitis is a murine autoimmune disease used to study multiple sclerosis. Parthenolide, a natural sesquiterpene lactone found inTanacetum partheniumL., is known for its strong anti-inflammatory activity. Herein, we have investigated thein vitroimmunomodulatory effects of parthenolide on cytokine production and nitric oxide in cultured cells from myelin oligodendrocyte glycoprotein 35-55 amino acid peptide mice. Experimental autoimmune encephalomyelitis was induced in C57BL/6 mice with myelin oligodendrocyte glycoprotein 35-55 amino acid peptide, and parthenolide was isolated fromT. parthenium. Splenocytes and peritoneal cells were obtained from experimental autoimmune encephalomyelitis-induced mice and incubated with parthenolide (1, 5, and 20 µM). Afterin vitrotreatment with parthenolide, supernatants were collected, and nitric oxide and cytokines were measured. The results suggested that parthenolide may regulate the activity of Th17 and Th1 cells, mainly by decreasing IL-17, TNF-α, and interferon gamma production. This modulation may be related to the lower levels of IL-12p40 and IL-6 after treatment with parthenolide. It was shown, for the first time, that parthenolide presentsin vitroimmunomodulatory effects on inflammatory mediators produced by cells from experimental autoimmune encephalomyelitis-induced mice.
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil.
authors.forename: Lara Soares Aleixo
authors.initials: LSA
authors.lastname: de Carvalho
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil.
authors.forename: Lívia Beatriz Almeida
authors.initials: LBA
authors.lastname: Fontes
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil.
authors.forename: Matheus Coutinho
authors.initials: MC
authors.lastname: Gazolla
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil.
authors.forename: Débora Dos Santos
authors.initials: DDS
authors.lastname: Dias
authors.affiliation: Departamento de Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, Brasil.
authors.forename: Maria Aparecida
authors.initials: MA
authors.lastname: Juliano
authors.affiliation: Departamento de Parasitologia, Microbiologia e Imunologia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil.
authors.forename: Gilson Costa
authors.initials: GC
authors.lastname: Macedo
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil.
authors.forename: José
authors.initials: J
authors.lastname: Otávio do Amaral Corrêa
authors.affiliation: Departamento de Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal de Juiz de Fora, Juiz de Fora, Brasil.
authors.forename: Ademar A
authors.initials: AA
authors.lastname: Da Silva Filho
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use*; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000188:drug therapy* / Q000276:immunology; D007111:Immunity, Cellular / Q000187:drug effects*; D007155:Immunologic Factors / Q000627:therapeutic use; D008810:Mice, Inbred C57BL; D009103:Multiple Sclerosis / Q000188:drug therapy* / Q000276:immunology; D010936:Plant Extracts / Q000627:therapeutic use*; D012717:Sesquiterpenes / Q000302:isolation & purification / Q000627:therapeutic use*; D013154:Spleen / Q000166:cytology; D006377:T-Lymphocytes, Helper-Inducer / Q000187:drug effects / Q000276:immunology; D020903:Tanacetum parthenium / Q000737:chemistry*
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D007155:Immunologic Factors; D010936:Plant Extracts; D012717:Sesquiterpenes; C002669:parthenolide