title: Effect of Several HIV Antigens Simultaneously Loaded with G2-NN16 Carbosilane Dendrimer in the Cell Uptake and Functionality of Human Dendritic Cells.
abstract: Dendrimers are highly branched, star-shaped, and nanosized polymers that have been proposed as new carriers for specific HIV-1 peptides. Dendritic cells (DCs) are the most-potent antigen-presenting cells that play a major role in the development of cell-mediated immunotherapy due to the generation and regulation of adaptive immune responses against HIV-1. This article reports on the associated behavior of two or three HIV-derived peptides simultaneously (p24/gp160 or p24/gp160/NEF) with cationic carbosilane dendrimer G2-NN16. We have found that (i) immature DCs (iDCs) and mature (mDCs) did not capture efficiently HIV peptides regarding the uptake level when cells were treated with G2-NN16-peptide complex alone; (ii) the ability of DCs to migrate was not depending on the peptides presence; and (iii) with the use of molecular dynamic simulation, a mixture of peptides decreased the cell uptake of the other peptides (in particular, NEF hinders the binding of more peptides and is especially obstructing of the binding of gp160 to G2-NN16). The results suggest that G2-NN16 cannot be considered as an alternative carrier for delivering two or more HIV-derived peptides to DCs.
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV-HGM BioBank , Madrid 28007, Spain.|Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN) , Madrid 28029, Spain.
authors.forename: Daniel
authors.initials: D
authors.lastname: Sepúlveda-Crespo
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV-HGM BioBank , Madrid 28007, Spain.|Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN) , Madrid 28029, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Vacas-Córdoba
authors.affiliation: Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Biología, Universidad Andres Bello , Av. República 239, Santiago 8320000, Chile.|Fundación Fraunhofer Chile Research , Las Condes 7550296, Chile.
authors.forename: Valeria
authors.initials: V
authors.lastname: Márquez-Miranda
authors.affiliation: Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Biología, Universidad Andres Bello , Av. República 239, Santiago 8320000, Chile.|Fundación Fraunhofer Chile Research , Las Condes 7550296, Chile.
authors.forename: Ingrid
authors.initials: I
authors.lastname: Araya-Durán
authors.affiliation: Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN) , Madrid 28029, Spain.|Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá Henares, Campus Universitario , Alcalá de Henares 28805, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Gómez
authors.affiliation: Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN) , Madrid 28029, Spain.|Departamento Química Orgánica y Química Inorgánica, Universidad de Alcalá Henares, Campus Universitario , Alcalá de Henares 28805, Spain.
authors.forename: Francisco Javier de la
authors.initials: FJ
authors.lastname: Mata
authors.affiliation: Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Biología, Universidad Andres Bello , Av. República 239, Santiago 8320000, Chile.|Fundación Fraunhofer Chile Research , Las Condes 7550296, Chile.|Centro Interdisciplinario de Neurociencia de Valparaíso, Facultad de Ciencias, Universidad de Valparaíso , Valparaíso 8370071, Chile.
authors.forename: Fernando Danilo
authors.initials: FD
authors.lastname: González-Nilo
authors.affiliation: Instituto de Investigación Sanitaria Gregorio Marañón (IiSGM), Spanish HIV-HGM BioBank , Madrid 28007, Spain.|Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN) , Madrid 28029, Spain.
authors.forename: Ma Ángeles
authors.identifier: http://orcid.org/0000-0002-0813-4500
authors.initials: MÁ
authors.lastname: Muñoz-Fernández
mesh_terms: D050091:Dendrimers / Q000737:chemistry* / Q000493:pharmacokinetics; D003713:Dendritic Cells / Q000187:drug effects*; D015488:HIV Antigens / Q000737:chemistry* / Q000494:pharmacology; D016655:HIV Core Protein p24 / Q000737:chemistry; D019302:HIV Envelope Protein gp160 / Q000737:chemistry; D006801:Humans; D056004:Molecular Dynamics Simulation; D012821:Silanes / Q000737:chemistry* / Q000493:pharmacokinetics; D055672:Static Electricity
chemical_list: C572050:2G-NN16; D050091:Dendrimers; D015488:HIV Antigens; D016655:HIV Core Protein p24; D019302:HIV Envelope Protein gp160; D012821:Silanes; C504072:carbosilane