title: Determination of anti-oxidative histidine dipeptides in poultry by microchip capillary electrophoresis with contactless conductivity detection.
abstract: A home-made microchip electrophoresis (MCE) device was used to quantitate two biologically important histidine dipeptides, carnosine and anserine, using capacitively coupled contactless conductivity detection (C4D), at pH 2.7. The C4D detector exhibited a linear response to both carnosine and anserine in the range of 0-200μM for the individual dipeptides and in the range of 0-100μM for each dipeptide when both were present as a mixture. The limit of detections (LOD) for the dipeptides in the mixture were 0.10μM for carnosine and 0.16μM for anserine. Standard addition was used to detemine the accuracy of the method. For carnosine and anserine the recoveries were in the range of 96.7±4.9-106.0±7.5% and 95.3±4.5-105.0±5.1% in thigh muscle and 97.5±5.1-105.0±7.5% and 95.3±5.4-97.3±5.6% in breast muscle, respectively.
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8A, HR-31000 Osijek, Croatia. Electronic address: mhorvat2@kemija.unios.hr.
authors.forename: Marija
authors.initials: M
authors.lastname: Jozanović
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8A, HR-31000 Osijek, Croatia. Electronic address: mhajdukovic@kemija.unios.hr.
authors.forename: Mateja
authors.initials: M
authors.lastname: Hajduković
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8A, HR-31000 Osijek, Croatia; Centre of Excellence, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia. Electronic address: ogalovic@kemija.unios.hr.
authors.forename: Olivera
authors.initials: O
authors.lastname: Galović
authors.affiliation: Centre of Excellence, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia; Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia. Electronic address: gkralik@pfos.hr.
authors.forename: Gordana
authors.initials: G
authors.lastname: Kralik
authors.affiliation: Centre of Excellence, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia; Faculty of Agriculture, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia. Electronic address: zlata.kralik@pfos.hr.
authors.forename: Zlata
authors.initials: Z
authors.lastname: Kralik
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8A, HR-31000 Osijek, Croatia. Electronic address: nikola.sakac@gmail.com.
authors.forename: Nikola
authors.initials: N
authors.lastname: Sakač
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8A, HR-31000 Osijek, Croatia. Electronic address: mmkosano@kemija.unios.hr.
authors.forename: Martina
authors.initials: M
authors.lastname: Medvidović-Kosanović
authors.affiliation: Department of Chemistry, Josip Juraj Strossmayer University of Osijek, Cara Hadrijana 8A, HR-31000 Osijek, Croatia; Centre of Excellence, Josip Juraj Strossmayer University of Osijek, HR-31000 Osijek, Croatia. Electronic address: msbosnar@kemija.unios.hr.
authors.forename: Milan
authors.initials: M
authors.lastname: Sak-Bosnar
mesh_terms: D000818:Animals; D000861:Anserine / Q000032:analysis*; D002336:Carnosine / Q000032:analysis*; D002645:Chickens; D019075:Electrophoresis, Capillary / Q000379:methods*; D018482:Muscle, Skeletal / Q000737:chemistry*
chemical_list: D002336:Carnosine; D000861:Anserine
keywords: Anserine; C(4)D detector; Carnosine; Histidine dipeptides; Microchip electrophoresis; Poultry meat; l-Anserine (PubChem CID: 112072); l-Carnosine (PubChem CID: 439224)