title: Effect of Low-Pressurized Perfusion with Different Concentration of Elastase on the Aneurysm Formation Rate in the Abdominal Aortic Aneurysm Model in Rabbits.
abstract: Establishing an animal model of abdominal aortic aneurysm (AAA) is the key to study the pathogenesis and the pathophysiological features of AAAs. We investigated the effects of low-pressurized perfusion with different concentrations of elastase on aneurysm formation rate in the AAA model. Fifty male New Zealand white rabbits were randomly divided into A, B, C, D, and E groups. 10μL of normal saline was perfused into the abdominal aorta in group A and 1 U/mL, 10 U/mL, 100 U/mL, or 200 U/mL of elastase was, respectively, perfused for the other four groups. All the animals were perfused for 7 min. Doppler ultrasound examinations of the abdominal aorta were performed before surgery and on day 14 after surgery. The rabbits were sacrificed and the perfused segment of the abdominal aorta was observed visually and after staining. The aneurysm formation rate of group A, group B, group C, group D, and group E was, respectively, 0%, 0%, 33.3%, 102.5-146.8%, and 241.5-255.2%. The survival rate of five groups was 90%, 90%, 90%, 90%, and 40%, respectively. So, we concluded that low-pressurized perfusion with 100 U/mL of elastase can effectively establish AAAs in rabbits with a high aneurysm formation rate.
authors.affiliation: Beijing Anzhen Hospital, Capital Medical University, Anzhen Road, Chaoyang District, Beijing 100029, China.
authors.forename: Maoxiao
authors.initials: M
authors.lastname: Nie
authors.affiliation: Beijing Anzhen Hospital, Capital Medical University, Anzhen Road, Chaoyang District, Beijing 100029, China.
authors.forename: Yunfeng
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Beijing Anzhen Hospital, Capital Medical University, Anzhen Road, Chaoyang District, Beijing 100029, China.
authors.forename: Xinhe
authors.identifier: 0000-0002-7149-0639
authors.initials: X
authors.lastname: Li
authors.affiliation: Beijing Anzhen Hospital, Capital Medical University, Anzhen Road, Chaoyang District, Beijing 100029, China.
authors.forename: Tingting
authors.identifier: 0000-0003-2126-8113
authors.initials: T
authors.lastname: Feng
authors.affiliation: Beijing Anzhen Hospital, Capital Medical University, Anzhen Road, Chaoyang District, Beijing 100029, China.
authors.forename: Xin
authors.identifier: 0000-0003-2809-3221
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Beijing Anzhen Hospital, Capital Medical University, Anzhen Road, Chaoyang District, Beijing 100029, China.
authors.forename: Mingduo
authors.identifier: 0000-0003-0111-4088
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Beijing Anzhen Hospital, Capital Medical University, Anzhen Road, Chaoyang District, Beijing 100029, China.
authors.forename: Quanming
authors.identifier: 0000-0003-3457-4953
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D000818:Animals; D001012:Aorta, Abdominal / Q000187:drug effects* / Q000473:pathology*; D017544:Aortic Aneurysm, Abdominal / Q000473:pathology*; D004195:Disease Models, Animal; D018450:Disease Progression; D004305:Dose-Response Relationship, Drug; D006801:Humans; D008297:Male; D010196:Pancreatic Elastase / Q000008:administration & dosage*; D011817:Rabbits; D015424:Reperfusion / Q000379:methods*
chemical_list: D010196:Pancreatic Elastase