title: [Synthesis and in vitro characterization of chitosan microspheres/ceramic bovine bone composite scaffold].
abstract: The chitosan microspheres encapsulated with bone morphogenetic protein-2 (BMP-2) were prepared by the emulsion cross-linking method. Then the chitosan microspheres were loaded in the ceramic bovine bone (CBB) to achieve the drug delivery system.
  
  
  
  The chemical structure and surface morphology of the drug delivery system were investigated by Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM) observation. Characterization preserved the loading capacity and encapsulation efficiency of the BMP-2. The dynamic immersion method was used to examine the in vitro release characteristic of BMP-2.
  
  
  
  The chitosan microspheres were successfully encapsulated BMP-2 by cross-linking method. The microspheres were micron-sized (5.982 μm) and spherical in shape with smooth surface morphology. From the release experiments, it was found that 5 mg chitosan/BMP-2 soaked for 21 days with a gradual release of BMP-2. The concentration of BMP-2 was (239.1±20.0) mg/L on Day 21. The in vitro experiment showed that this novel drug delivery system could accelerate MC3T3-E1 cells proliferation and osteogenic differentiation.
  
  
  
  The drug delivery system achieves the biological function of BMP-2 and sustaining slow release in bone repair parts. Also it can provide the basis for repair of bone tissue defect treatment and the selection of bone tissue engineering scaffolds.
authors.affiliation: Center of Digital Dentistry,Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Li
authors.affiliation: School of Biological Science and Medical Engineering,Beihang University, Beijing 100191, China.
authors.forename: X
authors.initials: X
authors.lastname: Yu
authors.affiliation: School of Biological Science and Medical Engineering,Beihang University, Beijing 100191, China.
authors.forename: G
authors.initials: G
authors.lastname: Zhou
authors.affiliation: The Second Dental Center, Peking University School and Hospital of Stomatology, Beijing 100101, China.
authors.forename: Y W
authors.initials: YW
authors.lastname: Wu
authors.affiliation: The Second Dental Center, Peking University School and Hospital of Stomatology, Beijing 100101, China.
authors.forename: H C
authors.initials: HC
authors.lastname: Hu
authors.affiliation: The Second Dental Center, Peking University School and Hospital of Stomatology, Beijing 100101, China.
authors.forename: T
authors.initials: T
authors.lastname: Wang
authors.affiliation: Center of Digital Dentistry,Peking University School and Hospital of Stomatology & National Engineering Laboratory for Digital and Material Technology of Stomatology & Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Tang
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2 / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D001861:Bone Regeneration / Q000187:drug effects; D002417:Cattle; D002516:Ceramics / Q000494:pharmacology; D048271:Chitosan / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D004339:Drug Compounding / Q000379:methods; D016503:Drug Delivery Systems / Q000379:methods; D066298:In Vitro Techniques; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: C525718:BMP2 protein, human; D055396:Bone Morphogenetic Protein 2; D048271:Chitosan