title: Photo-induced biosynthesis of silver nanoparticles from aqueous extract of Dunaliella salina and their anticancer potential.
abstract: The synthesis of silver nanoparticles (AgNPs) via green route, using biological entities is an area of interest, because one of the potential applications in the nanomedicine. In the present study, we have developed photo-induced, ecofriendly, low cost method for biosynthesis of the stable silver nanoparticles using aqueous extract of Dunaliella salina (AED) which act as both reducing as well as stabilizing agent. Biosynthesis of the AgNPs was optimized as: sunlight exposure (30min), AED (5% (v/v)) and AgNO3(4mM). Biosynthesis of AgNPs was monitored by using UV-Vis spectroscopy which exhibited sharp SPR band at 430nm after 30min of bright sunlight exposure. SEM and TEM analyses confirmed the presence of spherical AgNPs with average size of 15.26nm. Crystalline nature of AgNPs was confirmed by SAED and XRD analyses where Braggs reflection pattern at (111), (200), (220) and (311) corresponded to face centered cubic crystal lattice of metallic silver. FTIR analysis revealed the involvement of various functional groups present in AED. AFM analysis confirmed the average surface roughness of synthesized AgNPs as 8.48nm. AgNPs were also screened for anticancer potential using assay of calcein AM/PI, Annexin/PI and cancer biomarkers against cancer cell line (MCF-7), while normal cell line (MCF-10A) were kept as control. Interestingly, anticancer potential was comparable to the known anticancer drug (Cisplatin), and was not detrimental to the normal cell line. Therefore, such green synthesized AgNPs may be explored as anticancer agent.
authors.affiliation: R. N. Singh Memorial Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Ankit Kumar
authors.initials: AK
authors.lastname: Singh
authors.affiliation: Council Scientific and Industrial Research Indian Institute of Toxicology Research, Lucknow 226001, India.
authors.forename: Ratnakar
authors.initials: R
authors.lastname: Tiwari
authors.affiliation: Nanomaterial Research Laboratory, Department of Chemistry, IIT, BHU, Varanasi 221005, India.
authors.forename: Vijay
authors.initials: V
authors.lastname: Kumar
authors.affiliation: R. N. Singh Memorial Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Prabhakar
authors.initials: P
authors.lastname: Singh
authors.affiliation: R. N. Singh Memorial Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Riyazat Khadim
authors.affiliation: R. N. Singh Memorial Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India.
authors.forename: Anupam
authors.initials: A
authors.lastname: Tiwari
authors.affiliation: Council Scientific and Industrial Research Indian Institute of Toxicology Research, Lucknow 226001, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Srivastava
authors.affiliation: Nanomaterial Research Laboratory, Department of Chemistry, IIT, BHU, Varanasi 221005, India.
authors.forename: S H
authors.initials: SH
authors.lastname: Hasan
authors.affiliation: R. N. Singh Memorial Laboratory, Centre of Advanced Study in Botany, Banaras Hindu University, Varanasi 221005, India. Electronic address: rasthana@bhu.ac.in.
authors.forename: R K
authors.initials: RK
authors.lastname: Asthana
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000378:metabolism; D045744:Cell Line, Tumor; D000460:Chlorophyta / Q000378:metabolism*; D006801:Humans; D053768:Metal Nanoparticles; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014408:Biomarkers, Tumor; D010936:Plant Extracts; D012834:Silver
keywords: Anticancer activity; Apoptosis; Dunaliella salina; Green route; Photo-induced; Silver nanoparticles