title: Elevated THBS2, COL1A2, and SPP1 Expression Levels as Predictors of Gastric Cancer Prognosis.
abstract: OBJECTIVE
  Gastric cancer (GC) is an important health problem. Classification based on molecular subtypes may help to determine the prognosis of patients with GC. Tumor invasion and metastasis are important factors affecting the prognosis of cancer. We aimed to identify genes related to tumor invasion and metastasis, which may serve as indicators of good GC prognosis.
  
  
  METHODS
  Tumor tissues and adjacent normal tissues were collected from 105 patients with primary GC who were treated by undergoing radical surgery. Samples were used for tissue microarray analysis. Identified genes with altered expression were further analyzed using the Gene Ontology (Go) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The expression levels of THBS2, COL1A2 and SPP1 were analyzed by RT-PCR, western blot and immunohistochemistry. The overall survival curves of patients with high and low expression of each gene of interest were plotted and compared.
  
  
  RESULTS
  Forty-three genes were identified. THBS2, COL1A2 and SPP1 were selected for further analysis. Altered expression levels of THBS2, COL1A2 and SPP1 in tumor tissues were confirmed. Patients with low THBS2 expression had a better prognosis; the expression of COL1A2 and SPP1 might not affect the prognosis of patients with GC.
  
  
  CONCLUSIONS
  THBS2, but not COL1A2 and SPP1, may serve as an indicator of GC prognosis.
authors.affiliation: Department of Psychological Medicine, Wenzhou Seventh People's Hospital, Wenzhou, China.
authors.forename: Chuanjun
authors.initials: C
authors.lastname: Zhuo
authors.forename: Xubin
authors.initials: X
authors.lastname: Li
authors.forename: Hongqing
authors.initials: H
authors.lastname: Zhuang
authors.forename: Shunli
authors.initials: S
authors.lastname: Tian
authors.forename: Hailong
authors.initials: H
authors.lastname: Cui
authors.forename: Ronghuan
authors.initials: R
authors.lastname: Jiang
authors.forename: Chuanxin
authors.initials: C
authors.lastname: Liu
authors.forename: Ran
authors.initials: R
authors.lastname: Tao
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Lin
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D016000:Cluster Analysis; D024042:Collagen Type I / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D053495:Osteopontin / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D013274:Stomach Neoplasms / Q000235:genetics* / Q000473:pathology; D019699:Thrombospondins / Q000235:genetics* / Q000378:metabolism; D000099262:Thrombospondin 2
chemical_list: D024042:Collagen Type I; D053495:Osteopontin; D012333:RNA, Messenger; D019699:Thrombospondins; D000099262:Thrombospondin 2; C000601279:COL1A2 protein, human; C506895:SPP1 protein, human