title: The Binding Specificity of the PHD-Finger Domain of VIN3 Moderates Vernalization Response.
abstract: Vernalization is a response to winter cold to initiate flowering in spring. VERNALIZATION INSENSITIVE3 (VIN3) is induced by winter cold and is essential to vernalization response in Arabidopsis (Arabidopsis thaliana). VIN3 encodes a PHD-finger domain that binds to modified histones in vitro. An alteration in the binding specificity of the PHD-finger domain of VIN3 results in a hypervernalization response. The hypervernalization response is achieved by increased enrichments of VIN3 and trimethylation of Histone H3 Lys 27 at the FLC locus without invoking the increased enrichment of Polycomb Repressive Complex 2. Our result shows that the binding specificity of the PHD-finger domain of VIN3 plays a role in mediating a proper vernalization response in Arabidopsis.
authors.affiliation: Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712.
authors.forename: Dong-Hwan
authors.identifier: 0000-0003-3348-4948
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Molecular Biosciences and Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, Texas 78712 sbsung@austin.utexas.edu.
authors.forename: Sibum
authors.identifier: 0000-0003-2980-9009
authors.initials: S
authors.lastname: Sung
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002843:Chromatin / Q000235:genetics / Q000378:metabolism; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism; D026161:MADS Domain Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D063151:Polycomb Repressive Complex 2; D000072417:Protein Domains; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D002843:Chromatin; D004268:DNA-Binding Proteins; C418902:At5g10140 protein, Arabidopsis; D006657:Histones; D026161:MADS Domain Proteins; C574170:PRC2 protein, Arabidopsis; D012097:Repressor Proteins; D014157:Transcription Factors; C480553:VIN3 protein, Arabidopsis; D063151:Polycomb Repressive Complex 2; D008239:Lysine