title: miR-194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts.
abstract: MicroRNA-194 (miR-194), a typical p53 responsive miRNA, serves as a tumor suppressor similar as p53, and has been demonstrated to play an anti-proliferation role in various human cancers. In spite of the pivotal role of p53 during aging process, the knowledge of miR-194's contribution to cellular senescence is limited. We herein sought to explore the role of miR-194 in the replicative senescence and stress-induced senescence of mouse embryonic fibroblasts. Our results unraveled that, compared to young cells, miR-194 is highly expressed in senescent cells, and extra expression of miR-194 significantly triggers the replicative senescence of MEFs and H2O2-induced senescence of NIH/3T3 cells, while inhibition of miR-194 exhibited the opposite effect. We further unveiled that DNMT3A was a direct and authentic target of miR-194, which has been reported to be closely associated with cellular senescence. Taken together, our data suggest that miR-194 may significantly promote the development of cellular senescence in mouse embryonic fibroblasts, which potentially occurs through inhibiting the DNMT3A expression.
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Shun
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Bing
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Yanmei
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Haijiao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Wenping
authors.initials: W
authors.lastname: Yang
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Haiyong
authors.initials: H
authors.lastname: Huang
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Hui-Ling
authors.initials: HL
authors.lastname: Zheng
authors.affiliation: Institute of Aging Research, Guangdong Medical University, Xin Cheng Avenue 1#, Songshan Lake, Guangdong, 523808, P.R. China.|Provincial Key Laboratory of Medical Molecular Diagnostics, Guangdong Medical University, Guangdong, 523808, China.|Institute of Biochemistry and Molecular Biology, Guangdong Medical University, Zhanjiang, P.R. China.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
mesh_terms: D000818:Animals; D016922:Cellular Senescence / Q000502:physiology*; D005347:Fibroblasts / Q000502:physiology*; D057809:HEK293 Cells; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology; D006801:Humans; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics*; D041681:NIH 3T3 Cells
chemical_list: C554845:MIRN194 microRNA, mouse; D035683:MicroRNAs
keywords: DNMT3A; cellular senescence; miR-194