title: Early detection of Trichinella spiralis DNA in the feces of experimentally infected mice by using PCR.
abstract: The aim of this study was to detect Trichinella spiralis DNA in mouse feces during the early stages of infection using PCR. The target gene fragment, a 1.6kb repetitive sequence of T. spiralis genome, was amplified by PCR from feces of mice infected with 100 or 300 larvae at 3-24h post infection (hpi) and 2-28dpi. The sensitivity of PCR was 0.016 larvae in feces. The primers used were highly specific for T. spiralis. No cross-reactivity was observed with the DNA of other intestinal helminths. T. spiralis DNA was detected in 100% (12/12) of feces of mice infected with 100 or 300 larvae as early as 3hpi, with the peak detection lasting to 12-24hpi, and then fluctuating before declining gradually. By 28dpi, the detection rate of T. spiralis DNA in feces of the two groups of infected mice decreased to 8.33% and 25%, respectively. PCR detection of T. spiralis DNA in feces is simple and specific; it might be useful for the early diagnosis of Trichinella infection.
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xiao Lin
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Hua Nan
authors.initials: HN
authors.lastname: Ren
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Ya Li
authors.initials: YL
authors.lastname: Shi
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Chen Xi
authors.initials: CX
authors.lastname: Hu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Yan Yan
authors.initials: YY
authors.lastname: Song
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Jiang Yang
authors.initials: JY
authors.lastname: Duan
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Hui Ping
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Xin Rui
authors.initials: XR
authors.lastname: Du
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Ruo Dan
authors.initials: RD
authors.lastname: Liu
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China. Electronic address: wangzq@zzu.edu.cn.
authors.forename: Zhong Quan
authors.initials: ZQ
authors.lastname: Wang
authors.affiliation: Department of Parasitology, Medical College, Zhengzhou University, Zhengzhou 450052, China. Electronic address: cuij@zzu.edu.cn.
authors.forename: Jing
authors.initials: J
authors.lastname: Cui
mesh_terms: D000818:Animals; D017931:DNA Primers; D018509:DNA, Helminth / Q000032:analysis*; D042241:Early Diagnosis; D005243:Feces / Q000469:parasitology*; D005260:Female; D007814:Larva; D051379:Mice; D008807:Mice, Inbred BALB C; D016133:Polymerase Chain Reaction / Q000662:veterinary*; D017160:Trichinella spiralis / Q000235:genetics*; D014235:Trichinellosis / Q000175:diagnosis* / Q000469:parasitology
chemical_list: D017931:DNA Primers; D018509:DNA, Helminth
keywords: DNA; Early diagnosis; Feces; PCR; Trichinella spiralis