title: Methods for monitoring signaling molecules in cellular compartments.
abstract: Cells, irrespective of whether they are from multicellular or single-celled organisms, must communicate with the external environment through dynamic regulation of their internal metabolism, which are critical for their survival. Fluorescent and bioluminescent proteins, and related genetic engineering technologies, have provided new opportunities to investigate the molecular dynamics of cells and their internal compartments, with high spatio-temporal resolution. In this review article, since there is a sufficient number of previous reviews summarizing the history of their development and the techniques behind them, here we will focus on molecular features or technologies that have the potential to further open novel investigations of cellular and subcellular dynamics.
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan; Japan Science and Technology Agency, PRESTO, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Agetsuma
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan.
authors.forename: Tomoki
authors.initials: T
authors.lastname: Matsuda
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, Mihogaoka 8-1, Ibaraki, Osaka 567-0047, Japan. Electronic address: ng1@sanken.osaka-u.ac.jp.
authors.forename: Takeharu
authors.initials: T
authors.lastname: Nagai
mesh_terms: D000818:Animals; D002451:Cell Compartmentation; D006801:Humans; D008163:Luminescent Measurements; D056004:Molecular Dynamics Simulation; D057054:Molecular Imaging / Q000379:methods*; D015398:Signal Transduction; D013347:Subcellular Fractions / Q000378:metabolism
keywords: Bioimaging; Bioluminescent protein; FRET; Optogenetics; Super resolution microscopy; pH sensitivity