title: Proteomic analysis of the compatible interaction of wheat and powdery mildew (Blumeria graminis f. sp. tritici).
abstract: Proteome characteristics of wheat leaves with the powdery mildew pathogen Blumeria graminis f. sp. tritici (Bgt) infection were investigated by two-dimensional electrophoresis and tandem MALDI-TOF/TOF-MS. We identified 46 unique proteins which were differentially expressed at 24, 48, and 72 h post-inoculation. The functional classification of these proteins showed that most of them were involved in photosynthesis, carbohydrate and nitrogen metabolism, defense responses, and signal transduction. Upregulated proteins included primary metabolism pathways and defense responses, while proteins related to photosynthesis and signal transduction were mostly downregulated. As expected, more antioxidative proteins were activated at the later infection stage than the earlier stage, suggesting that the antioxidative system of host plays a role in maintaining the compatible interaction between wheat and powdery mildew. A high accumulation of 6-phosphogluconate dehydrogenase and isocitrate dehydrogenase in infected leaves indicated the regulation of the TCA cycle and pentose phosphate pathway in parallel to the activation of host defenses. The downregulation of MAPK5 could be facilitated for the compatible interaction of wheat plants and Bgt. qRT-PCR analysis supported the data of protein expression profiles. Our results reveal the relevance of primary plant metabolism and defense responses during compatible interaction, and provide new insights into the biology of susceptible wheat in response to Bgt infection.
authors.affiliation: Key Laboratory of Regulating and Controlling Crop Growth and Development (Henan Agriculture University) Ministry of Education; Collaborative Innovation Center of Henan Grain Crops; College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Regulating and Controlling Crop Growth and Development (Henan Agriculture University) Ministry of Education; Collaborative Innovation Center of Henan Grain Crops; College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Xiwen
authors.initials: X
authors.lastname: Yang
authors.affiliation: Lab of Plant Protection, Kaifeng Agriculture and Forestry Science Institute, Kaifeng, Henan 475004, China.
authors.forename: Xinhao
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Regulating and Controlling Crop Growth and Development (Henan Agriculture University) Ministry of Education; Collaborative Innovation Center of Henan Grain Crops; College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Yu
authors.affiliation: Key Laboratory of Regulating and Controlling Crop Growth and Development (Henan Agriculture University) Ministry of Education; Collaborative Innovation Center of Henan Grain Crops; College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Congyang
authors.initials: C
authors.lastname: Du
authors.affiliation: Key Laboratory of Regulating and Controlling Crop Growth and Development (Henan Agriculture University) Ministry of Education; Collaborative Innovation Center of Henan Grain Crops; College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450002, China.
authors.forename: Mengda
authors.initials: M
authors.lastname: Li
authors.affiliation: Key Laboratory of Regulating and Controlling Crop Growth and Development (Henan Agriculture University) Ministry of Education; Collaborative Innovation Center of Henan Grain Crops; College of Agronomy, Henan Agricultural University, Zhengzhou, Henan 450002, China. Electronic address: hedexian@126.com.
authors.forename: Dexian
authors.initials: D
authors.lastname: He
mesh_terms: D001203:Ascomycota / Q000502:physiology*; D015169:Colony Count, Microbial; D015180:Electrophoresis, Gel, Two-Dimensional; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D010935:Plant Diseases / Q000382:microbiology*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods*; D060888:Real-Time Polymerase Chain Reaction; D014908:Triticum / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D010940:Plant Proteins
keywords: Compatible interaction; Defense responses; Leaf; Powdery mildew; Primary metabolism; Triticum aestivum