title: Degradation of phytosterols in tobacco waste extract by a novel Paenibacillus sp.
abstract: Phytosterols have been demonstrated to be precursors of polycyclic aromatic hydrocarbons (PAHs) formed during biomass pyrolysis. Here, a novel Paenibacillus sp. was evaluated for its ability to degrade phytosterols in tobacco waste extract (TWE). The optimal conditions for cell growth and stigmasterol (a representative of phytosterols) degradation were 37 °C, pH 7.0, 1.0 g/L yeast extract, and 6.0 g/L glucose. Paenibacillus sp. could degrade stigmasterol under high concentrations of glucose (up to 130 g/L) and tolerate wide pH (5.0-9.0) and temperature (25-42 °C) ranges. The new strain could degrade stigmasterol completely into CO2and H2O, and no intermediate steroids were detected during the degradation process. Phytosterol degradation in TWE was demonstrated by high-performance liquid chromatography-tandem mass spectrometry. Under optimal conditions (37 °C, pH 7.0, with the exponential-phase cells), the total degradation ratio of phytosterols reached 38.5% in TWE, including 45.2% of stigmasterol, 37.4% of β-sitosterol, 27.3% of campesterol, and 28.7% of cholesterol. These results showed that Paenibacillus sp. is a candidate for phytosterol degradation in TWE and other biomass and is potentially useful in reducing the PAHs generated from biomass pyrolysis.
authors.affiliation: School of Food and Biological Engineering, Henan Provincial Collaborative Innovation Center for Food Production and Safety, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, People's Republic of China.
authors.forename: Jianbin
authors.initials: J
authors.lastname: Ye
authors.affiliation: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, People's Republic of China.
authors.forename: Zhan
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Food and Biological Engineering, Henan Provincial Collaborative Innovation Center for Food Production and Safety, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, People's Republic of China.
authors.forename: Ji
authors.initials: J
authors.lastname: Yan
authors.affiliation: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Hao
authors.affiliation: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, People's Republic of China.
authors.forename: Xiangzhen
authors.initials: X
authors.lastname: Liu
authors.affiliation: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, People's Republic of China.
authors.forename: Zongcan
authors.initials: Z
authors.lastname: Yang
authors.affiliation: School of Food and Biological Engineering, Henan Provincial Collaborative Innovation Center for Food Production and Safety, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, People's Republic of China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.affiliation: School of Food and Biological Engineering, Henan Provincial Collaborative Innovation Center for Food Production and Safety, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, People's Republic of China.
authors.forename: Xuepeng
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Food and Biological Engineering, Henan Provincial Collaborative Innovation Center for Food Production and Safety, Zhengzhou University of Light Industry, Zhengzhou, Henan Province, People's Republic of China.
authors.forename: Duobin
authors.initials: D
authors.lastname: Mao
authors.affiliation: Technology Center, China Tobacco Henan Industrial Co., Ltd., Zhengzhou, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhou
mesh_terms: D049109:Cell Proliferation; D002851:Chromatography, High Pressure Liquid; D006863:Hydrogen-Ion Concentration; D056507:Paenibacillus / Q000166:cytology / Q000378:metabolism; D010802:Phylogeny; D010840:Phytosterols / Q000737:chemistry / Q000302:isolation & purification / Q000378:metabolism*; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism*; D053719:Tandem Mass Spectrometry; D013696:Temperature; D014026:Nicotiana / Q000737:chemistry / Q000378:metabolism*
chemical_list: D010840:Phytosterols; D010936:Plant Extracts
keywords: HPLC-MS/MS; Paenibacillus sp; phytosterol degradation; tobacco waste extract