title: Lipid production with Trichosporon oleaginosus in a membrane bioreactor using microalgae hydrolysate.
abstract: Lipid production by Trichosporon oleaginosus was first studied in fed-batch operated stirred-tank bioreactors on a milliliter- and liter-scale making use of typical sugar monomers and a sugar mixture that may be derived from microalgae biomass hydrolysis after the extraction of lipids. 20.3gL-1lipids (58% of dry cell mass) were produced with T. oleaginosus in a defined medium at nitrogen starvation in the fed-batch process with a typical microalgae derived carbohydrate mixture (60% glucose, 20% mannose, 20% galactose). Real microalgae hydrolysate resulted in superior growth of T. oleaginosus but no enhanced lipid formation was possible due to nitrogen and phosphorus excess in the hydrolysate. Phosphate precipitation and the application of a continuously operated membrane bioreactor with total cell retention due to the low sugar concentrations (∼40gL-1) in the microalgae hydrolysate resulted in the production of 30gL-1lipids (53% of dry cell mass) with T. oleaginosus at high space-time-yields of 0.33g lipids L-1h-1. A high apparent lipid yield of 0.43g lipids g-1sugars consumed (130% of the theoretical maximum) was achieved with the microalgae hydrolysate most likely due to the additional conversion of other carbon sources (e.g. uronic acids, peptides) in the hydrolysate.
authors.affiliation: Institute of Biochemical Engineering, Technical University of Munich, Boltzmannstraße 15, 85748 Garching, Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Meo
authors.affiliation: Institute of Biochemical Engineering, Technical University of Munich, Boltzmannstraße 15, 85748 Garching, Germany.
authors.forename: Xenia Laura
authors.initials: XL
authors.lastname: Priebe
authors.affiliation: Institute of Biochemical Engineering, Technical University of Munich, Boltzmannstraße 15, 85748 Garching, Germany. Electronic address: d.weuster-botz@lrz.tum.de.
authors.forename: Dirk
authors.initials: D
authors.lastname: Weuster-Botz
mesh_terms: D018533:Biomass; D019149:Bioreactors / Q000382:microbiology*; D008055:Lipids / Q000032:analysis / Q000096:biosynthesis*; D058086:Microalgae / Q000378:metabolism*; D014250:Trichosporon / Q000378:metabolism*
chemical_list: D008055:Lipids
keywords: Trichosporon oleaginosus; membrane bioreactor; microalgae hydrolysate; microbial lipids; total cell retention