title: Interferon-Tau has Antiproliferative effects, Represses the Expression of E6 and E7 Oncogenes, Induces Apoptosis in Cell Lines Transformed with HPV16 and Inhibits Tumor GrowthIn Vivo.
abstract: Interferon tau (IFN-τ) is a promising alternative antiviral and immunotherapeutic agent in a wide variety of diseases including infectious, neurodegenerative, autoimmune and cancer due to its low toxicity in comparison with other type I interferon´s. The objective of our study was established the effect of the bovine IFN-τ on human (SiHa) and murine (BMK-16/myc) cells transformed with HPV 16 and evaluates the antitumor effect in a murine tumor model HPV 16 positive. We determine that bovine IFN-τ has antiproliferative effects, pro-apoptotic activity and induces repression of viral E6 and E7 oncogenes (time- and dose-dependent) on human and murine cells transformed with HPV 16 similar to the effects of IFN-β. However, IFN-τ induces greater antiproliferative effect, apoptosis and repression of both oncogenes in BMK-16/myc cells compared to SiHa cells. The differences were explained by the presence and abundance of the type I interferon receptor(IFNAR)in each cell line. On the other hand, we treated groups of tumor-bearing mice (HPV16 positive) with IFN-τ and showed the inhibition tumor growth effectin vivo. Our finding indicates that bovine IFN-τ may be a good candidate for immunotherapy against cervical cancer.
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Herbey Oswaldo
authors.initials: HO
authors.lastname: Padilla-Quirarte
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Cesar
authors.initials: C
authors.lastname: Trejo-Moreno
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Geny
authors.initials: G
authors.lastname: Fierros-Zarate
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Jhoseline Carnalla
authors.initials: JC
authors.lastname: Castañeda
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Marie
authors.initials: M
authors.lastname: Palma-Irizarry
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Eva
authors.initials: E
authors.lastname: Hernández-Márquez
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Ana Isabel
authors.initials: AI
authors.lastname: Burguete-Garcia
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Oscar
authors.initials: O
authors.lastname: Peralta-Zaragoza
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Vicente
authors.initials: V
authors.lastname: Madrid-Marina
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Kirvis
authors.initials: K
authors.lastname: Torres-Poveda
authors.affiliation: Division of Chronic Infection and Cancer, National Institute of Public Health, Av. Universidad 655, Cuernavaca, Morelos. Mexico, 62100.
authors.forename: Victor Hugo
authors.initials: VH
authors.lastname: Bermúdez-Morales
keywords: Human papillomavirus; IFN-τ; immunotherapy.; oncogenes