title: Does Cryopreservation Affect the Biological Properties of Stem Cells from Dental Tissues? A Systematic Review.
abstract: This systematic review evaluated if different cryopreservation protocols could affect biological properties (Cell survival rate (CSR), proliferation, differentiation, maintenance of stem cell markers) of stem cells obtained from dental tissues (DSC) post-thaw. An electronic search was carried out within PubMed and ISI Web Science by using specific keyword. Two independent reviewers read the titles and abstracts of all reports respecting predetermined inclusion/exclusion criteria. Data were extracted considering the biological properties of previously cryopreserved DSCs and previously cryopreserved dental tissues. DSCs cryopreserved as soon as possible after their isolation presents a CSR quite similar to the non-cryopreserved DSC. Dimethyl sulfoxide (DMSO) [10%] showed good results related to cell recovery post-thaw to cryopreserve cells and tissues for periods of up to 2 years. The cryopreservation of DSC in a mechanical freezer (-80°C) allows the recovery of stem cells post-thaw. The facilities producing magnetic field (MF), demand a lower concentration of cryoprotectant, but their use is not dispensable. It is possible to isolate and cryopreserve dental pulp stem cell (DPSC) from healthy and diseased vital teeth. Cryopreservation of dental tissues for late DSC isolation, combined with MF dispensability, could be valuable to reduce costs and improve the logistics to develop teeth banks.
authors.affiliation: Post-Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Marcus Cristian Muniz
authors.initials: MC
authors.lastname: Conde
authors.affiliation: Post-Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Luiz Alexandre
authors.initials: LA
authors.lastname: Chisini
authors.affiliation: Post-Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Grazioli
authors.affiliation: School of Dentistry, University of the Republic, Montevideo, Uruguay.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Francia
authors.affiliation: School of Dentistry - Faculdade Meridional IMED, Passo Fundo, Brazil.
authors.forename: Rodrigo Varella de
authors.initials: RV
authors.lastname: Carvalho
authors.affiliation: Post-Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.|Post-Graduate Program in Science and Material Engineering, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Jose Carlos Bernedo
authors.initials: JC
authors.lastname: Alcázar
authors.affiliation: Post-Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.|Department of Semiology and Clinics, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Sandra Beatriz Chavez
authors.initials: SB
authors.lastname: Tarquinio
authors.affiliation: Post-Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas, Pelotas, Brazil.|Post-Graduate Program in Epidemiology, Federal University of Pelotas, Pelotas, Brazil.
authors.forename: Flávio Fernando
authors.initials: FF
authors.lastname: Demarco
mesh_terms: D002470:Cell Survival; D015925:Cryopreservation; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D014070:Tooth / Q000166:cytology*