title: Further analysis reveals new gut microbiome markers of type 2 diabetes mellitus.
abstract: In recent years, metagenome-wide association studies have revealed potential relationships between intestinal microbiomes and the pathogenesis of type 2 diabetes mellitus (T2DM). However, considering the increase in volume of gene catalogues and algorithms, an updated analysis would be expected to confirm previous discoveries and provide new knowledge. We therefore constructed new profiles after mapping the recent catalogue of reference genes in the human gut microbiome to reanalyze samples from T2DM cases and controls in the Chinese population. We identified different compositions between Chinese controls and T2DM patients at the species and genus levels, especially in the case of butyrate-producing bacteria, Haemophilus, and Lactobacillus. An effective metagenomic linkage group random forest model was built to differentiate controls from T2DM cases in different cohorts. Functional markers from the Kyoto Encyclopedia of Genes and Genomes database were identified using new annotations. We also report 16 virulence factor markers and 22 antibiotic resistance markers associated with T2DM.
authors.affiliation: BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, China. viruswater@hotmail.com.|BGI-Shenzhen, Shenzhen, 518083, China. viruswater@hotmail.com.
authors.forename: Xiaokai
authors.initials: X
authors.lastname: Wang
authors.affiliation: BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, China.|BGI-Shenzhen, Shenzhen, 518083, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Xu
authors.affiliation: BGI Education Center, University of Chinese Academy of Sciences, Shenzhen, China.|BGI-Shenzhen, Shenzhen, 518083, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
mesh_terms: D002681:China / Q000453:epidemiology; D015331:Cohort Studies; D003924:Diabetes Mellitus, Type 2 / Q000453:epidemiology / Q000382:microbiology*; D005243:Feces / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D064349:Genome, Microbial; D006801:Humans; D054892:Metagenome; D056186:Metagenomics / Q000379:methods
chemical_list: D005819:Genetic Markers
keywords: Antibiotic resistance; Gut microbiome; Metagenome; T2DM; Virulence factor