title: Detection and differentiation of influenza viruses with glycan-functionalized gold nanoparticles.
abstract: Accurate diagnosis of influenza viruses is difficult and generally requires a complex process because of viral diversity and rapid mutability. In this study, we report a simple and rapid strategy for the detection and differentiation of influenza viruses using glycan-functionalized gold nanoparticles (gGNPs). This method is based on the aggregation of gGNP probes on the viral surface, which is mediated by the specific binding of the virus to the glycans. Using a set of gGNPs bearing different glycan structures, fourteen influenza virus strains, including the major subtypes currently circulating in human and avian populations, were readily differentiated from each other and from a human respiratory syncytial virus in a single-step colorimetric procedure. The results presented here demonstrate the potential of this gGNP-based system in the development of convenient and portable sensors for the clinical diagnosis and surveillance of influenza viruses.
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; Savaid Medical School, University of Chinese Academy of Sciences, Huairou District, Beijing 101408, China.
authors.forename: Longtang
authors.initials: L
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences (CAS), Zhongguancun, Beijing 100190, China.
authors.forename: Jinhua
authors.initials: J
authors.lastname: Wei
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Lv
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE), Chaoyang District, Beijing 100101, China.
authors.forename: Yuhai
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Lanzhou Institute of Animal Science and Veterinary Pharmaceutics, Chinese Academy of Agricultural Science, Lanzhou 730050, China.
authors.forename: Peixing
authors.initials: P
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; Savaid Medical School, University of Chinese Academy of Sciences, Huairou District, Beijing 101408, China.
authors.forename: Zhenxing
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; Savaid Medical School, University of Chinese Academy of Sciences, Huairou District, Beijing 101408, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China.
authors.forename: Ruichen
authors.initials: R
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; Savaid Medical School, University of Chinese Academy of Sciences, Huairou District, Beijing 101408, China.
authors.forename: Jingwen
authors.initials: J
authors.lastname: Jiang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China.
authors.forename: Haolong
authors.initials: H
authors.lastname: Cong
authors.affiliation: Core Facility, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China.
authors.forename: Jingnan
authors.initials: J
authors.lastname: Liang
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Zhongguancun, Beijing 100190, China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Chen
authors.affiliation: National Glycoengineering Research Center, School of Pharmaceutical Science, Shandong University, Jinan 250012, China.
authors.forename: Hongzhi
authors.initials: H
authors.lastname: Cao
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE), Chaoyang District, Beijing 100101, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE), Chaoyang District, Beijing 100101, China.
authors.forename: George F
authors.initials: GF
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Biochemical Engineering, PLA Key Laboratory of Biopharmaceutical Production & Formulation Engineering, Institute of Process Engineering, Chinese Academy of Sciences (CAS), Zhongguancun, Beijing 100190, China.
authors.forename: Yuguang
authors.initials: Y
authors.lastname: Du
authors.affiliation: CAS Key Lab for Biological Effects of Nanomaterials and Nanosafety, National Center for Nanoscience and Technology, Zhongguancun, Beijing 100190, China.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
authors.affiliation: CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences (CAS), Chaoyang District, Beijing 100101, China; National Engineering Research Center for Carbohydrate Synthesis, Jiangxi Normal University, Nanchang 330022, China; Savaid Medical School, University of Chinese Academy of Sciences, Huairou District, Beijing 101408, China; Center for Influenza Research and Early-warning, Chinese Academy of Sciences (CASCIRE), Chaoyang District, Beijing 100101, China. Electronic address: lixb@im.ac.cn.
authors.forename: Xuebing
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000379:methods; D001717:Birds / Q000821:virology; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006801:Humans; D005585:Influenza in Birds / Q000821:virology; D007251:Influenza, Human / Q000821:virology; D029521:Alphainfluenzavirus / Q000145:classification / Q000302:isolation & purification*; D029524:Betainfluenzavirus / Q000145:classification / Q000302:isolation & purification*; D053758:Nanoparticles / Q000737:chemistry*; D009976:Orthomyxoviridae Infections / Q000821:virology*; D000067716:Point-of-Care Testing; D011134:Polysaccharides / Q000737:chemistry*
chemical_list: D011134:Polysaccharides; D006046:Gold
keywords: Detection; Differentiation; Glycan; Gold nanoparticles; Influenza virus