title: Screening of Potential Free Radicals Scavenger and Antibacterial Activities of Purwoceng (Pimpinella alpinaMolk).
abstract: Purwoceng (Pimpinella alpinaMolk) is a traditional medicinal plant used for its aphrodisiac values. This plant was originated Dieng Plateu, Central Java, Indonesia. Purwoceng has been reported to contain steroid, flavonoids, glycoside, saponins, tannins, and phenolic. Based on secondary metabolite compounds of Purwoceng herbs, a research need to be done to determine the other potential free radicals scavenger and antibacterial activities of Purwoceng. The objectives of this research are to screen the potential free radicals scavenger activity of in vitro using DPPH (1,1 diphenyl-2-picryl-hydrazil) radicals and NO• (nitric oxide) radicals, and antibacterial activity of Purwoceng. The extraction is done by a maceration method with petroleum ether, ethyl acetate, and ethanol solvent, respectively. Free radicals scavenger test was performed using DPPH radicals and NO• radicals, while antibacterial activity screening was performed using agar diffusion test. The results showed that ethyl acetate extract of Purwoceng has free radical scavenger activity with IC50 53.07 ppm lower than butylated hydroxytoluene. Ethyl acetate extract and ethanol extract of Purwoceng have antibacterial activity againstStaphyloccus aureus,Escherichia coli, and MG42 bacterial isolate.
authors.affiliation: Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Indonesia.
authors.forename: Retno
authors.initials: R
authors.lastname: Wahyuningrum
authors.affiliation: Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Indonesia.
authors.forename: Pri Iswati
authors.initials: PI
authors.lastname: Utami
authors.affiliation: Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Indonesia.
authors.forename: Binar Asrining
authors.initials: BA
authors.lastname: Dhiani
authors.affiliation: Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Indonesia.
authors.forename: Malikhah
authors.initials: M
authors.lastname: Kumalasari
authors.affiliation: Faculty of Pharmacy, Universitas Muhammadiyah Purwokerto, Indonesia.
authors.forename: Rizka Sari
authors.initials: RS
authors.lastname: Kusumawardani
keywords: Antibacterial; Purwoceng; Radical Scavenger