title: Chemical inhibition of the histone acetyltransferase activity in Arabidopsis thaliana.
abstract: Chemical inhibition of chromatin regulators provides an effective approach to investigate the roles of chromatin modifications in plant and animals. In this work, chemical inhibition of the Arabidopsis histone acetyltransferase activity by γ-butyrolactone (MB-3), the inhibitor of the catalytic activity of mammalian GENERAL CONTROL NON-REPRESSIBLE 5 (GCN5) is evaluated. Arabidopsis seedlings were germinated in LS medium supplemented with different concentrations of MB-3, and inhibition in the root length and yellowed leaves were observed. The yellowed leaves phenotype of the plants grown in 100 μM of MB-3 was reverted when plants were additionally treated with 1 μM of TSA, a histone deacetylase inhibitor. Using an immunoblot assay with specific antibodies revealed a reduction of H3K14 acetylation levels at 3 and 24 h post-treatment. At 24 h post-treatment a reduction of H3K9 acetylation levels was observed. Targets of GCN5 related to stress were downregulated at 3 h post-treatment but no change was observed in target genes related to developmental transition. Our results indicate that MB-3 is a chemical inhibitor of the histone acetyltransferase in Arabidopsis and suggest that this inhibitor could function in other plants species.
authors.affiliation: Laboratorio de Bioingeniería, Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Santiago, Chile; Center for Applied Ecology and Sustainability (CAPES), Santiago, Chile. Electronic address: felipe.aquea@uai.cl.
authors.forename: Felipe
authors.initials: F
authors.lastname: Aquea
authors.affiliation: Laboratorio de Bioingeniería, Facultad de Ingeniería y Ciencias, Universidad Adolfo Ibáñez, Santiago, Chile.
authors.forename: Tania
authors.initials: T
authors.lastname: Timmermann
authors.affiliation: Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Chile.
authors.forename: Ariel
authors.initials: A
authors.lastname: Herrera-Vásquez
mesh_terms: D015107:4-Butyrolactone / Q000494:pharmacology*; D000107:Acetylation / Q000187:drug effects; D000595:Amino Acid Sequence; D017360:Arabidopsis / Q000201:enzymology* / Q000254:growth & development; D029681:Arabidopsis Proteins / Q000037:antagonists & inhibitors* / Q000737:chemistry; D020134:Catalytic Domain; D015536:Down-Regulation; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D018525:Germination / Q000187:drug effects; D051548:Histone Acetyltransferases / Q000037:antagonists & inhibitors* / Q000737:chemistry; D006657:Histones / Q000378:metabolism; D006877:Hydroxamic Acids / Q000494:pharmacology; D000099224:p300-CBP-Associated Factor
chemical_list: D015107:4-Butyrolactone; D029681:Arabidopsis Proteins; D004791:Enzyme Inhibitors; D051548:Histone Acetyltransferases; D006657:Histones; D006877:Hydroxamic Acids; C012589:trichostatin A; C474636:GCN5 protein, Arabidopsis; D000099224:p300-CBP-Associated Factor
keywords: Arabidopsis; Chemical inhibitor; Chromatin remodeling; GCN5; Histone acetyltransferase