title: Structural elucidation and immunostimulating property of a novel polysaccharide extracted from an edible mushroom Lentinus fusipes.
abstract: A water soluble heteroglycan (PS-II) with an average molecular weight∼60kDa was isolated from the hot aqueous extract of an edible mushroom Lentinus fusipes. The structural characterization of PS-II was carried out using total acid hydrolysis, methylation analyses, periodate oxidation, Smith degradation and 1D/2D NMR experiments. Total acid hydrolysis indicated the presence of D-galactose and D-glucose in a molar ratio of approximately 1:1. The chemical and NMR analyses revealed that the proposed repeating unit of the PS-II had a backbone chain consisting of three (1→6)-linked α-d-galactopyranosyl residue and two (1→6)-linked β-d-glucopyranosyl residues, one of the β-d-glucopyranosyl residue was branched at O-3 position with a terminal β-d-glucopyranosyl. The PS-II exhibited significant in vitro splenocyte and macrophage activations with optimum dose of 20μg/ml and 80μg/ml respectively. Flow cytometry study revealed the protective role of the PS-II against nicotine stimulated lymphocytes. Moreover, the ROS scavenging property of PS-II was also established using DPPH radical scavenging assay.
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Dilip K
authors.initials: DK
authors.lastname: Manna
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Maity
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Ashis K
authors.initials: AK
authors.lastname: Nandi
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Manabendra
authors.initials: M
authors.lastname: Pattanayak
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Bibhash C
authors.initials: BC
authors.lastname: Panda
authors.affiliation: Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India.
authors.forename: Amit K
authors.initials: AK
authors.lastname: Mandal
authors.affiliation: Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102, West Bengal, India.
authors.forename: Satyajit
authors.initials: S
authors.lastname: Tripathy
authors.affiliation: Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India.
authors.forename: Krishnendu
authors.initials: K
authors.lastname: Acharya
authors.affiliation: Regional Plant Resource Centre, Bhubaneswar 751015, Odisha, India.
authors.forename: Atish K
authors.initials: AK
authors.lastname: Sahoo
authors.affiliation: Regional Plant Resource Centre, Bhubaneswar 751015, Odisha, India.
authors.forename: Nibha
authors.initials: N
authors.lastname: Gupta
authors.affiliation: Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102, West Bengal, India.
authors.forename: Somnath
authors.initials: S
authors.lastname: Roy
authors.affiliation: Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India. Electronic address: sirajul_1999@yahoo.com.
authors.forename: Syed S
authors.initials: SS
authors.lastname: Islam
mesh_terms: D000363:Agaricales; D016166:Free Radical Scavengers / Q000737:chemistry; D006801:Humans; D007166:Immunosuppressive Agents / Q000737:chemistry*; D020074:Lentinula / Q000737:chemistry*; D008214:Lymphocytes / Q000187:drug effects*; D009682:Magnetic Resonance Spectroscopy; D010084:Oxidation-Reduction; D011134:Polysaccharides / Q000737:chemistry* / Q000276:immunology
chemical_list: D016166:Free Radical Scavengers; D007166:Immunosuppressive Agents; D011134:Polysaccharides
keywords: Biological properties; Heteroglycan; Lentinus fusipes; NMR studies