title: In vitroevaluation of the sealing ability of three newly developed root canal sealers: A bacterial microleakage study.
abstract: BACKGROUND
  The purpose of this study was to compare the sealing ability of MTA Fillapex, Apatite Root Canal Sealer and AH26 sealers.
  
  
  METHODS
  The presentin vitrostudy was carried out on 142 extracted single-rooted human mature teeth. The teeth were randomly divided into three experimental groups (n=44) and two control groups (n=5). Three root canal sealers were MTA Fillapex, Apatite Root Canal Sealer and AH26. The teeth in the control groups were either filled with no sealer or made completely impermeable. The root canals were prepared and obturated with gutta-percha and one of the sealers. The teeth were sterilized with ethylene oxide gas prior to the bacterial leakage assessment usingEnterococcus faecalis. Leakage was evaluated every 24 hours for 90 days. Data were analyzed with descriptive statistical methods and chi-squared test. If the data were significant, a proper post hoc test was used. Statistical significance was set atP<0.05.
  
  
  RESULTS
  The positive control specimens exhibited total bacterial penetration whilst the negative control specimens showed no evidence of bacterial penetration. At the end of the study, the analysis of microleakage with chi-squared test showed no significant differences between the experimental groups (P<0.05). The results of chi-squared test analyzing the pair-wise differences between the groups considering the numerical values for leakage day indicated the lowest leakage with AH26 and the highest with Apatite root sealer.
  
  
  CONCLUSIONS
  According to the results of the present study, sealing ability of AH26 was significantly higher than that of MTA Fillapex and Apatite Root Canal Sealer.Key words:Mineral Trioxide aggregate, root canal obturation, dental seal.
authors.affiliation: Assistant Professor, Endodontics Department, Dentistry Faculty, Tabriz University of Medical Sciences.
authors.forename: Farnaz
authors.initials: F
authors.lastname: Jafari
authors.affiliation: Dentist, Dentistry Faculty, Tabriz University of Medical Sciences.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Sobhani
authors.affiliation: Assistant Professor, Drug Applied Reserach Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Samadi-Kafil
authors.affiliation: Assistant Professor, Oral health and community Dentistry Department, Dentistry Faculty, Tabriz University of Medical Sciences.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Pirzadeh
authors.affiliation: Assistant Professor, Orthodontics Department, Dentistry Faculty, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Sanaz
authors.initials: S
authors.lastname: Jafari