title: Characterization of Gastric Microbiota in Twins.
abstract: Contribution of host genetic backgrounds in the development of gastric microbiota has not been clearly defined. This study was aimed to characterize the biodiversity, structure and composition of gastric microbiota among twins. A total of four pairs of twins and eight unrelated individuals were enrolled in the study. Antral biopsies were obtained during endoscopy. The bacterial 16S rRNA gene was amplified and pyrosequenced. Sequences were analyzed for the composition, structure, and α and β diversities of gastric microbiota. Proteobacteria, Firmicutes, Bacteroidetes, Actinobacteria, and Fusobacteria were the most predominant phyla of gastric microbiota. Each individual, twins as well as unrelated individuals, harbored a microbiota of distinct composition. There was no evidence of additional similarity in the richness and evenness of gastric microbiota among co-twins as compared to unrelated individuals. Calculations of θYCand PCoA demonstrated that the structure similarity of gastric microbial community between co-twins did not increase compared to unrelated individuals. In contrast, the structure of microbiota was altered enormously by Helicobacter pylori infection. These results suggest that host genetic backgrounds had little effect in shaping the gastric microbiota. This property of gastric microbiota could facilitate the studies discerning the role of microbiota from genetic grounds in the pathogenesis.
authors.affiliation: Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China.
authors.forename: Quanjiang
authors.initials: Q
authors.lastname: Dong
authors.affiliation: Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China.
authors.forename: Yongning
authors.initials: Y
authors.lastname: Xin
authors.affiliation: Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Meng
authors.affiliation: Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China.
authors.forename: Xinjuan
authors.initials: X
authors.lastname: Yu
authors.affiliation: Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China.
authors.forename: Linlin
authors.initials: L
authors.lastname: Lu
authors.affiliation: Central Laboratories and Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, 266000, Shandong, China. jiangacer@126.com.
authors.forename: Shiying
authors.initials: S
authors.lastname: Xuan
mesh_terms: D001419:Bacteria / Q000145:classification* / Q000235:genetics*; D044822:Biodiversity; D001706:Biopsy; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D064307:Microbiota; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013270:Stomach / Q000382:microbiology*; D014427:Twins
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S