title: Immune Responses Induced by Recombinant Bacillus Subtilis Expressing the Hemagglutinin Protein of H5N1 in chickens.
abstract: To develop an effective, safe, and convenient vaccine for the prevention of highly pathogenic avian influenza (HPAI) H5N1, we have constructed a recombinant Bacillus subtilis strain (B.S.-HA) expressing the hemagglutinin (HA) protein. Then we evaluated the immune function in chicken bone marrow derived dendritic cells (BM-DCs), and the immune response after oral immunization. Our results show that the recombinant Bacillus subtilis B.S.-HA could be sampled by BM-DCs in vitro and increase the BM-DCs major histocompatibility complex (MHC) II phenotype. The weight, height of the small intestine villus, and lymphoid tissue area of the ileum increased significantly in B.S.-HA immunized chickens (P < 0.05 or P < 0.01). B.S.-HA induced the secretion of cytokines and the expression of Toll-like receptors in the trachea and small intestine (P < 0.05 or P < 0.01). In addition, B.S.-HA elevated the specific IgA titers in the trachea, IgG and HI antibody titers in serum (P < 0.05 or P < 0.01). Therefore, B.S.-HA provides a potential novel strategy and approach for developing an H5N1 vaccine.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Mou
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Liqi
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Yang
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Wenwen
authors.initials: W
authors.lastname: Xu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, Weigang 1, Nanjing, Jiangsu, 210095, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001412:Bacillus subtilis / Q000502:physiology*; D001835:Body Weight / Q000187:drug effects; D001854:Bone Marrow Cells / Q000378:metabolism; D002478:Cells, Cultured; D002645:Chickens / Q000276:immunology* / Q000382:microbiology* / Q000821:virology; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000494:pharmacology; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000378:metabolism*; D007114:Immunization; D053124:Influenza A Virus, H5N1 Subtype / Q000502:physiology*; D015847:Interleukin-4 / Q000494:pharmacology; D007421:Intestine, Small / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism; D008221:Lymphoid Tissue / Q000187:drug effects; D010957:Plasmids / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism; D011995:Recombination, Genetic / Q000235:genetics*; D013154:Spleen / Q000166:cytology; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D011993:Recombinant Fusion Proteins; D051193:Toll-Like Receptors; D015847:Interleukin-4; D016178:Granulocyte-Macrophage Colony-Stimulating Factor