title: Infliximab does not increase colonic cancer risk associated to murine chronic colitis.
abstract: OBJECTIVE
  To explore the influence of Infliximab (IFX) on cancer progression in a murine model of colonic cancer associated to chronic colitis.
  
  
  METHODS
  AOM/DSS model was induced in C57BL/6 mice. Mice were injected with IFX (5 mg/kg) during each DSS cycle while control mice received saline. Body weight, occult blood test and stool consistency were measured to calculate the disease activity index (DAI). Mice were sacrificed at week 10 and colons were analyzed macroscopically and microscopically for number of cancers and degree of inflammation. MTT assay was performed on CT26 to evaluate the potential IFX role on metabolic activity and proliferation. Cells were incubated with TNF-α or IFX or TNF-α plus IFX, and cell vitality was evaluated after 6, 24 and 48 h. The same setting was used after pre-incubation with TNF-α for 24 h.
  
  
  RESULTS
  IFX significantly reduced DAI and body weight loss in mice compared with controls, preserving also colon length at sacrifice. Histological score was also reduced in treated mice. At macroscopic analysis, IFX treated mice showed a lower number of tumor lesions compared to controls. This was confirmed at microscopic analysis, although differences were not statistically significant.In vitro, IFX treated CT26 maintained similar proliferation ability at MTT test, both when exposed to IFX alone and when associated to TNF-α.
  
  
  CONCLUSIONS
  IFX did not increase colonic cancer risk in AOM-DSS model of cancer on chronic colitis nor influence directly the proliferation of murine colon cancer epithelial cells.
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Loris R
authors.initials: LR
authors.lastname: Lopetuso
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Petito
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Tiziano
authors.initials: T
authors.lastname: Zinicola
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Graziani
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Viviana
authors.initials: V
authors.lastname: Gerardi
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Arena
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Maria Emiliana
authors.initials: ME
authors.lastname: Caristo
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Poscia
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Cammarota
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Alfredo
authors.initials: A
authors.lastname: Papa
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Valerio
authors.initials: V
authors.lastname: Cufino
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Alessandro
authors.initials: A
authors.lastname: Sgambato
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Gasbarrini
authors.affiliation: Loris R Lopetuso, Valentina Petito, Tiziano Zinicola, Cristina Graziani, Viviana Gerardi, Giovanni Cammarota, Alfredo Papa, Valerio Cufino, Antonio Gasbarrini, Franco Scaldaferri, Gastroenterology Division, Catholic University of Sacred Heart, 00168 Rome, Italy.
authors.forename: Franco
authors.initials: F
authors.lastname: Scaldaferri
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology* / Q000633:toxicity; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002908:Chronic Disease; D003092:Colitis / Q000139:chemically induced / Q000473:pathology / Q000517:prevention & control*; D003106:Colon / Q000187:drug effects* / Q000473:pathology; D003110:Colonic Neoplasms / Q000209:etiology* / Q000473:pathology / Q000517:prevention & control; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D005765:Gastrointestinal Agents / Q000494:pharmacology* / Q000633:toxicity; D000069285:Infliximab / Q000494:pharmacology* / Q000633:toxicity; D008810:Mice, Inbred C57BL; D013997:Time Factors; D014409:Tumor Necrosis Factor-alpha / Q000494:pharmacology
chemical_list: D000893:Anti-Inflammatory Agents; D005765:Gastrointestinal Agents; D014409:Tumor Necrosis Factor-alpha; D016264:Dextran Sulfate; D000069285:Infliximab
keywords: AOM-DSS model; Cancer on chronic colitis; Colorectal cancer; Inflammatory bowel disease; Infliximab; Ulcerative colitis