title: Regulation of PPARγ and CIDEC expression by adenovirus 36 in adipocyte differentiation.
abstract: This study is to investigate the role of adenovirus 36 (Ad36) in regulating expression of peroxisome proliferator-activated receptor γ (PPARγ) and cell death-inducing DFFA-like effector c (CIDEC) in Ad36-induced adipocyte differentiation. Human adipose-derived mesenchymal stem cells (hAMSCs) were isolated and cultured, and then infected with Ad36. Ad36-induced adipocytes were identified using quantitative real-time PCR and Oil red O staining. The expression levels of PPARγ and CIDEC in Ad36-induced adipocytes were determined by quantitative real-time PCR and Western blot analysis. Glucose uptake and intracellular triglyceride content were also determined in these induced cells. Our results from the Oil red O staining showed that Ad36 induced the differentiation of hAMSCs into human adipocytes in vitro. Moreover, the medium glucose concentration was significantly decreased, while the intracellular triglyceride content was significantly increased, in the Ad36-induced adipocytes, compared with the control group. Furthermore, our results showed that, the mRNA and protein expression levels of PPARγ and CIDEC were significantly upregulated in Ad36-induced adipocytes, in a time-dependent manner. On the other hand, compared with the control group, the CIDEC expression was downregulated when the Ad36-induced adipocytes were treated with the PPARγ inhibitor, GW9662. Ad36 could upregulate the expression level of CIDEC through increasing PPARγ expression during the adipocyte differentiation process.
authors.affiliation: Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiao
authors.affiliation: Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China.
authors.forename: Yiliyasi
authors.initials: Y
authors.lastname: Aisa
authors.affiliation: Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China.
authors.forename: Xiaodi
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China.
authors.forename: Nuerbiye
authors.initials: N
authors.lastname: Nuermaimaiti
authors.affiliation: Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China.
authors.forename: Xian
authors.initials: X
authors.lastname: Gong
authors.affiliation: Medical Testing Center, The First Affiliated Hospital of Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China. xia00513@aliyun.com.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Biochemistry, Preclinical Medicine College, Xinjiang Medical University, No. 393 Xinyi Road, Ürümqi, 830011, Xinjiang, China. miranda812@163.com.|Diabetes Center, The First Affiliated Hospital of Xinjiang Medical University, Ürümqi, 830011, Xinjiang, China. miranda812@163.com.
authors.forename: Yaqun
authors.initials: Y
authors.lastname: Guan
mesh_terms: D000256:Adenoviridae / Q000502:physiology*; D017667:Adipocytes / Q000166:cytology* / Q000378:metabolism / Q000821:virology; D000813:Anilides / Q000494:pharmacology; D051017:Apoptosis Regulatory Proteins; D002454:Cell Differentiation; D002478:Cells, Cultured; D005947:Glucose / Q000378:metabolism; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000821:virology*; D047495:PPAR gamma / Q000235:genetics* / Q000378:metabolism; D011506:Proteins / Q000235:genetics* / Q000378:metabolism; D013997:Time Factors; D014280:Triglycerides / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: C457499:2-chloro-5-nitrobenzanilide; D000813:Anilides; D051017:Apoptosis Regulatory Proteins; C472088:CIDEC protein, human; D047495:PPAR gamma; D011506:Proteins; D014280:Triglycerides; D005947:Glucose
keywords: Adenovirus 36 (Ad36); CIDEC; Human adipose-derived mesenchymal stem cell (hAMSCs); PPARγ