title: A fluorescence-quenching method for quantitative analysis of Ponceau 4R in beverage.
abstract: CdTe quantum dots was synthesized and used to quantitative analysis of Ponceau 4R in solution. With the excitation wavelength of 380nm the emission of CdTe quantum dots was quenched obviously by Ponceau 4R. In order to detect Ponceau 4R in mild condition the influences of fluorescence emission wavelength of CdTe quantum dots, pH value, temperature and reaction time were examined to establish the experimental condition. The linear response of the fluorescence intensity of CdTe quantum dots to Ponceau 4R allowed the quantitative analysis of Ponceau 4R in a range of 2.5-25μg/mL, and the limit of detection for Ponceau 4R was 0.025μg/mL. In addition, the responsive mechanism of this reaction system was investigated in detail by using the modified Stern-Volmer equation and thermodynamic calculation. Particularly, this method was used to quantitatively analyze the real sample, which indicated that this method could be more widely applied in similar samples.
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, PR China.
authors.forename: Jianpo
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Synthetic Rubber, Chinese Academy of Sciences, Changchun Institute of Applied Chemistry, Changchun 130022, PR China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Na
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, PR China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Han
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, PR China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Lou
authors.affiliation: School of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, PR China. Electronic address: canoe8013@126.com.
authors.forename: Li
authors.initials: L
authors.lastname: Jin
mesh_terms: D001391:Azo Compounds / Q000032:analysis*; D001628:Beverages / Q000032:analysis*; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D009282:Naphthalenesulfonates / Q000032:analysis*; D045663:Quantum Dots; D013050:Spectrometry, Fluorescence / Q000379:methods*
chemical_list: D001391:Azo Compounds; D009282:Naphthalenesulfonates; C576297:ponceau 4R
keywords: CdTe quantum dots; Food additive; Ponceau 4R; Sensor