title: Structural Basis for the Selective Pb(II) Recognition of Metalloregulatory Protein PbrR691.
abstract: The transcription regulator PbrR691, one of the MerR family proteins, shows extremely high sensitivity and selectivity toward Pb(II) in Ralstonia metallidurans CH34. Here, we present the crystal structure of PbrR691 in complex with Pb(II) at 2.0 Å resolution. The Pb(II) coordinates with three conserved cysteines and adopts a unique trigonal-pyramidal (hemidirected) geometry. To our knowledge, the PbrR691-Pb(II) structure provides the first three-dimensional visualization of a functional hemidirected lead(II) thiolate coordinate geometry in a protein.
authors.forename: Shanqing
authors.identifier: http://orcid.org/0000-0003-0504-9447
authors.initials: S
authors.lastname: Huang
authors.forename: Xichun
authors.initials: X
authors.lastname: Liu
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.forename: Weizhong
authors.initials: W
authors.lastname: Chen
authors.forename: Qingyuan
authors.initials: Q
authors.lastname: Hu
authors.forename: Tianbiao
authors.initials: T
authors.lastname: Wei
authors.forename: Wenquan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: School of Life Sciences, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Gan
authors.forename: Hao
authors.identifier: http://orcid.org/0000-0001-8937-4946
authors.initials: H
authors.lastname: Chen
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism*; D018360:Crystallography, X-Ray; D004268:DNA-Binding Proteins / Q000737:chemistry / Q000378:metabolism*; D007854:Lead / Q000378:metabolism*; D015394:Molecular Structure; D014157:Transcription Factors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C051124:MerR protein, Bacteria; D014157:Transcription Factors; D007854:Lead