title: Methylation-Sensitive Melt Curve Analysis of the Reprimo Gene Methylation in Gastric Cancer.
abstract: Reprimo (RPRM) is a p53-induced tumor suppressor gene. Its aberrant DNA methylation is correlated with carcinogenesis and may be used as a surrogate marker for the early detection of gastric cancer. However, the detail information regarding its DNA methylation has not been revealed. Here, we investigated the RPRM gene methylation in gastric cancer tumor and plasma samples by methylation-sensitive melt curve analysis (MS-MCA) and bisulfite sequencing in depth. We developed a semi-quantitative method based on MS-MCA for detecting DNA methylation and unraveled the RPRM gene methylation pattern in gastric cancer. This study provides a solid foundation for the future application of detecting RPRM gene methylation in human plasma or serum samples to help diagnose gastric cancer or for prognosis evaluation.
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, Fujian, China.
authors.forename: Hanze
authors.identifier: http://orcid.org/0000-0002-6278-8257
authors.initials: H
authors.lastname: Wang
authors.affiliation: The First Affiliated Hospital of Fujian Medical University, Fujian, China.
authors.forename: Yansong
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, Fujian, China.
authors.forename: Junzhong
authors.initials: J
authors.lastname: Lai
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, Fujian, China.
authors.forename: Qianping
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, Fujian, China.
authors.forename: Huican
authors.initials: H
authors.lastname: Ke
authors.affiliation: Fujian Key Laboratory of Innate Immune Biology, Biomedical Research Center of South China, College of Life Science, Fujian Normal University Qishan Campus, Fujian, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Chen
mesh_terms: D018797:Cell Cycle Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D019175:DNA Methylation; D044127:Epigenesis, Genetic; D006023:Glycoproteins / Q000235:genetics*; D006801:Humans; D011379:Prognosis; D011401:Promoter Regions, Genetic; D017422:Sequence Analysis, DNA / Q000379:methods*; D013274:Stomach Neoplasms / Q000235:genetics*
chemical_list: D018797:Cell Cycle Proteins; D006023:Glycoproteins; C412566:RPRM protein, human