title: Isolation, Identification and Screening of Potential Probiotic Bacteria in Milk from South African Saanen Goats.
abstract: This study aimed to evaluate lactic acid bacteria isolates from Saanen goats' milk for probiotic attributes, thereby determining their potential as direct-fed microbials for goats. Isolates were identified using API 50CH system, 16S rDNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry. All 17 isolates obtained were identified as Lactobacillus plantarum except one identified as Pediococcus acidilactici. Four isolates identified as L. plantarum (Accession numbers KJ026587.1, KM207826.1, KC83663.1 and KJ958428.1) by at least two of the techniques used and isolate 17 differently identified by all the methods used were selected as representatives and then screened for probiotic properties. These isolates displayed phenotypic probiotic attributes including tolerance to acid and bile salts, ability to adhere to intestines and possession of antagonistic activities against Proteus vulgaris, Staphylococcus aureus, Salmonella typhimurium, Pseudomonas aeruginosa and Escherichia coli. The lactic acid bacteria isolated from Saanen goats' milk showed potential to be used as sustainable probiotics in goats' industry. Successful use of probiotics in animals depends upon availability of appropriate isolates originating from the specific host animal. This study is a positive contribution towards identification of isolates with potential for formulation as direct-fed microbials for South African Saanen goats.
authors.affiliation: Department of Microbiology and Plant Pathology, New Agricultural Sciences building, University of Pretoria, Pretoria, 0001, South Africa. makete@arc.agric.za.|Agricultural Research Council-Animal Production Institute, Gastrointestinal Microbiology and Biotechnology Division, Irene, 0062, South Africa. makete@arc.agric.za.
authors.forename: Goitsemang
authors.identifier: 0000-0002-0783-3166
authors.initials: G
authors.lastname: Makete
authors.affiliation: Agricultural Research Council-Animal Production Institute, Gastrointestinal Microbiology and Biotechnology Division, Irene, 0062, South Africa.
authors.forename: Olayinka A
authors.initials: OA
authors.lastname: Aiyegoro
authors.affiliation: Department of Microbiology and Plant Pathology, New Agricultural Sciences building, University of Pretoria, Pretoria, 0001, South Africa.
authors.forename: Mapitsi S
authors.initials: MS
authors.lastname: Thantsha
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001422:Bacterial Adhesion; D001647:Bile Acids and Salts / Q000378:metabolism; D015169:Colony Count, Microbial; D024901:Drug Resistance, Multiple, Bacterial; D005506:Food Contamination; D005516:Food Microbiology; D006041:Goats; D007422:Intestines / Q000382:microbiology; D048191:Lactiplantibacillus plantarum / Q000302:isolation & purification / Q000502:physiology; D050296:Microbial Viability; D008892:Milk / Q000382:microbiology*; D000070016:Pediococcus acidilactici / Q000302:isolation & purification / Q000502:physiology; D019936:Probiotics / Q000302:isolation & purification*; D013019:South Africa; D013211:Staphylococcus aureus / Q000302:isolation & purification / Q000502:physiology
chemical_list: D000900:Anti-Bacterial Agents; D001647:Bile Acids and Salts
keywords: Direct-fed microbials; Lactic acid bacteria; Lactobacillus; Probiotics; Saanen goats