title: Analysis of Dithiocarbamate Fungicides in Vegetable Matrices Using HPLC-UV Followed by Atomic Absorption Spectrometry.
abstract: A simple method combining ion-pair methylation, high-performance liquid chromatography (HPLC) analysis with detection at 272 nm and atomic absorption spectrometry was developed in order to determine 10 dithiocarbamate fungicides (Dazomet, Metam-sodium, Ferbam, Ziram, Zineb, Maneb, Mancozeb, Metiram, Nabam and Propineb) and distinguish ethylenbisdithiocarbamates (EBDTCs) Zineb, Maneb and Mancozeb in diverse matrices. This method associates reverse phase analysis by HPLC analysis with detection at 272 nm, with atomic absorption spectrometry in order to distinguish, with the same extraction protocol, Maneb, Mancozeb and Zineb. The limits of detection (0.4, 0.8, 0.5, 1.25 and 1.97) and quantification (1.18, 2.5, 1.52, 4.2 and 6.52) calculated in injected nanogram, respectively, for Dazomet, Metam-Na, dimethyldithiocarbamates (DMDTCs), EBDTCs and propylenebisdithiocarbamates (PBDTCs) justify the sensitivity of the method used. The coefficients of determination R2 were 0.9985, 0.9978, 0.9949, 0.988 and 0.9794, respectively, for Dazomet, Metam-Na, DMDTCs, EBDTCs and PBDTCs, and the recovery from fortified apple and leek samples was above 90%. Results obtained with the atomic absorption method in comparison with spectrophotometric analysis focus on the importance of the atomic absorption as a complementary specific method for the distinction between different EBDTCs fungicides.
authors.affiliation: Azm Center for Research in Biotechnology and its Applications, Lebanese University, Tripoli, Lebanon.
authors.forename: Josephine
authors.initials: J
authors.lastname: Al-Alam
authors.affiliation: Chemistry, University of Strasbourg/CNRS, Rue Blaise Pascal, Strasbourg Cedex 67000, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Bom
authors.affiliation: Azm Center for Research in Biotechnology and its Applications, Lebanese University, Tripoli, Lebanon.
authors.forename: Asma
authors.initials: A
authors.lastname: Chbani
authors.affiliation: Azm Center for Research in Biotechnology and its Applications, Lebanese University, Tripoli, Lebanon.
authors.forename: Ziad
authors.initials: Z
authors.lastname: Fajloun
authors.affiliation: Chemistry, University of Strasbourg/CNRS UMR 7515 ICPEES, 1 Rue Blessig, Strasbourg Cedex 67084, France.
authors.forename: Maurice
authors.initials: M
authors.lastname: Millet
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004125:Dimethyldithiocarbamate / Q000032:analysis* / Q000737:chemistry; D005659:Fungicides, Industrial / Q000032:analysis* / Q000737:chemistry; D057230:Limit of Detection; D015203:Reproducibility of Results; D013054:Spectrophotometry, Atomic / Q000379:methods*; D014675:Vegetables / Q000737:chemistry*
chemical_list: D004125:Dimethyldithiocarbamate; D005659:Fungicides, Industrial