title: Jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to Rice black streaked dwarf virus infection in rice.
abstract: Plant hormones play a vital role in plant immune responses. However, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot-virus defense systems. We used a high-throughput-sequencing approach to compare the global gene expression of Rice black-streaked dwarf virus (RBSDV)-infected rice plants with that of healthy plants. Exogenous hormone applications and transgenic rice were used to test RBSDV infectivity and pathogenicity. Our results revealed that the jasmonic acid (JA) pathway was induced while the brassinosteroid (BR) pathway was suppressed in infected plants. Foliar application of methyl jasmonate (MeJA) or brassinazole (BRZ) resulted in a significant reduction in RBSDV incidence, while epibrassinolide (BL) treatment increased RBSDV infection. Infection studies using coi1-13 and Go mutants demonstrated JA-mediated resistance and BR-mediated susceptibility to RBSDV infection. A mixture of MeJA and BL treatment resulted in a significant reduction in RBSDV infection compared with a single BL treatment. MeJA application efficiently suppressed the expression of BR pathway genes, and this inhibition depended on the JA coreceptor OsCOI1. Collectively, our results reveal that JA-mediated defense can suppress the BR-mediated susceptibility to RBSDV infection.
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.|State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Yuqing
authors.initials: Y
authors.lastname: He
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.|College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
authors.forename: Hehong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Zongtao
authors.initials: Z
authors.lastname: Sun
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Junmin
authors.initials: J
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Gaojie
authors.initials: G
authors.lastname: Hong
authors.affiliation: Shandong Rice Research Institute, Jinan, 250100, China.
authors.forename: Qisong
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Shandong Rice Research Institute, Jinan, 250100, China.
authors.forename: Xuebiao
authors.initials: X
authors.lastname: Zhou
authors.affiliation: The James Hutton Institute, Cell and Molecular Sciences Group, Invergowrie, Dundee, DD2 5DA, UK.
authors.forename: Stuart
authors.initials: S
authors.lastname: MacFarlane
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yan
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Ministry of Agriculture Key Laboratory of Biotechnology in Plant Protection, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Chen
mesh_terms: D000085:Acetates / Q000494:pharmacology; D060406:Brassinosteroids / Q000494:pharmacology*; D003517:Cyclopentanes / Q000494:pharmacology*; D004198:Disease Susceptibility; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000821:virology*; D054883:Oxylipins / Q000494:pharmacology*; D010935:Plant Diseases / Q000821:virology*; D057865:Plant Immunity / Q000187:drug effects; D018515:Plant Leaves / Q000187:drug effects / Q000821:virology; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D010942:Plant Viruses / Q000187:drug effects / Q000502:physiology*; D015398:Signal Transduction / Q000187:drug effects; D013312:Stress, Physiological / Q000187:drug effects / Q000235:genetics; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000085:Acetates; D060406:Brassinosteroids; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; C011006:jasmonic acid; C072239:methyl jasmonate
keywords: Oryza sativa (rice); Rice Black Streaked Dwarf Virus (RBSDV); brassinosteroid pathway; jasmonic acid pathway; plant defense response