title: Development of a Multiplex Real-Time PCR for Determination of Apricot in Marzipan Using the Plexor System.
abstract: Marzipan is a confectionary which is mostly offered in form of filled chocolate, pralines, or pure. According to the German guidelines for oil seeds only almonds, sugar and water are admitted ingredients of marzipan. A product very similar in taste is persipan which is used in the confectionary industry because of its stronger flavor. For persipan production almonds are replaced by debittered apricot or peach kernels. To guarantee high quality products for consumers, German raw paste producers have agreed a limit of apricot kernels in marzipan raw paste of 0.5%. Different DNA-based methods for quantitation of persipan contaminations in marzipan are already published. To increase the detection specificity compared to published intercalation dye-based assays, the present work demonstrate the utilization of a multiplex real-time PCR based on the Plexor technology. Thus, the present work enables the detection of at least 0.1% apricot DNA in almond DNA or less. By analyzing DNA mixtures, the theoretical limit of quantification of the duplex PCR for the quantitation of persipan raw paste DNA in marzipan raw paste DNA was determined as 0.05%.
authors.affiliation: Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Schelm
authors.affiliation: Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Ilka
authors.initials: I
authors.lastname: Haase
authors.affiliation: Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Christin
authors.initials: C
authors.lastname: Fischer
authors.affiliation: Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg , Grindelallee 117, 20146 Hamburg, Germany.
authors.forename: Markus
authors.identifier: http://orcid.org/0000-0001-7243-4199
authors.initials: M
authors.lastname: Fischer
mesh_terms: D002182:Candy / Q000032:analysis*; D017931:DNA Primers; D005504:Food Analysis / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D000068219:Prunus armeniaca / Q000235:genetics*; D000068181:Prunus dulcis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results
chemical_list: D017931:DNA Primers
keywords: Plexor technology; Prunus; marzipan; multiplex real-time PCR; persipan