title: Scaffolds of hydroxyl apatite nanoparticles disseminated in 1, 6-diisocyanatohexane-extended poly(1, 4-butylene succinate)/poly(methyl methacrylate) for bone tissue engineering.
abstract: Poly(1, 4-butyl succinate) extended 1, 6-diisocyanatohexane (PBSu-DCH) polymers and Polymethylmethacrylate (PMMA) scaffolds decorated with nano hydroxyl apatite have been prepared and characterized for regeneration of bone in cranio-maxillofacial region. Synthesized scaffolds revealed good response in bone regeneration and excellent cell viability in comparison to commercial available glass plate, which lead to better proliferation of MG-63 cell lines. Additionally, they demonstrate high porosity and excellent water retention ability. Moreover, controlled degradation (in pH=7.4) and sustained drug release in pH (4.5 and 7.4) are advantages of these scaffolds to serve as delivery vehicles for therapeutic drugs. Samples also provide the protection against Escherichia coli and Methicillin Resistant Staphylococcus aureus microorganisms which can be helpful for quick recovery of the patient. In-vitro inflammatory response has been assessed via adsorption of human plasma/serum proteins on the surface of the scaffolds. Results suggest that prepared scaffolds have good bone regeneration ability and provide friendly environment for the cell growth with the additional advantage of protection of the surrounding tissues from microbial infection. With all these features, it is speculated that these scaffolds will have wide utility in the area of tissue engineering and regenerative medicine.
authors.affiliation: Department of Physics, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Kulwinder
authors.initials: K
authors.lastname: Kaur
authors.affiliation: Department of Physics, Guru Nanak Dev University, Amritsar 143005, India. Electronic address: kanwarjitsingh@yahoo.com.
authors.forename: K J
authors.initials: KJ
authors.lastname: Singh
authors.affiliation: Department of Physics, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Anand
authors.affiliation: Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Bhatia
authors.affiliation: Department of Molecular Biology and Biochemistry, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Raminderjit
authors.initials: R
authors.lastname: Kaur
authors.affiliation: Department of Human Genetics, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Manpreet
authors.initials: M
authors.lastname: Kaur
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Lovedeep
authors.initials: L
authors.lastname: Nim
authors.affiliation: Department of Microbiology, Guru Nanak Dev University, Amritsar 143005, India.
authors.forename: Daljit Singh
authors.initials: DS
authors.lastname: Arora
mesh_terms: D000327:Adsorption; D000475:Alkenes / Q000737:chemistry*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001798:Blood Proteins / Q000378:metabolism; D001861:Bone Regeneration; D001842:Bone and Bones / Q000502:physiology*; D002072:Butylene Glycols / Q000737:chemistry*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D065546:Drug Liberation; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D017953:Isocyanates / Q000737:chemistry*; D007700:Kinetics; D053758:Nanoparticles / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry; D014961:X-Ray Diffraction
chemical_list: D000475:Alkenes; D000900:Anti-Bacterial Agents; D001798:Blood Proteins; D002072:Butylene Glycols; D017953:Isocyanates; D011108:Polymers; C089797:bionole; D014867:Water; C015262:1,6-hexamethylene diisocyanate; C558934:butylene; D019904:Polymethyl Methacrylate; D017886:Durapatite
keywords: Hydroxyl apatite; Scaffold; Tissue engineering