title: Colonic and Hepatic Modulation by Lipoic Acid and/or N-Acetylcysteine Supplementation in Mild Ulcerative Colitis Induced by Dextran Sodium Sulfate in Rats.
abstract: Lipoic acid (LA) andN-acetylcysteine (NAC) are antioxidant and anti-inflammatory agents that have not yet been tested on mild ulcerative colitis (UC). This study aims to evaluate the action of LA and/or NAC, on oxidative stress and inflammation markers in colonic and hepatic rat tissues with mild UC, induced by dextran sodium sulfate (DSS) (2% w/v). LA and/or NAC (100 mg·kg·day-1, each) were given, once a day, in the diet, in a pretreatment phase (7 days) and during UC induction (5 days). Colitis induction was confirmed by histological and biochemical analyses (high performance liquid chromatography, spectrophotometry, and Multiplex®). A redox imbalance occurred before an immunological disruption in the colon. NAC led to a decrease in hydrogen peroxide (H2O2), malondialdehyde (MDA) levels, and myeloperoxidase activity. In the liver, DSS did not cause damage but treatments with both antioxidants were potentially harmful, with LA increasing MDA and LA + NAC increasing H2O2, tumor necrosis factor alpha, interferon gamma, and transaminases. In summary, NAC exhibited the highest colonic antioxidant and anti-inflammatory activity, while LA + NAC caused hepatic damage.
authors.affiliation: Faculdade de Nutrição (FANUT), Universidade Federal de Alagoas (UFAL), Campus A. C. Simões, Tabuleiro dos Martins, 57072-970 Maceió, AL, Brazil; Pós-Graduação em Ciências da Saúde (PPGCS/UFAL), Maceió, AL, Brazil.
authors.forename: Fabiana Andréa
authors.initials: FA
authors.lastname: Moura
authors.affiliation: Pós-Graduação em Ciências da Saúde (PPGCS/UFAL), Maceió, AL, Brazil.
authors.forename: Kívia Queiroz
authors.initials: KQ
authors.lastname: de Andrade
authors.affiliation: Instituto de Química e Biotecnologia (IQB/UFAL), Maceió, AL, Brazil.
authors.forename: Orlando Roberto Pimentel
authors.initials: OR
authors.lastname: de Araújo
authors.affiliation: Laboratório de Reatividade Cardiovascular (LRC/UFAL), Maceió, AL, Brazil; Departamento de Fisiologia e Farmacologia (DFF), Centro de Biociências (CB), Universidade Federal de Pernambuco (UFPE), Av. Prof. Moraes Rego, 1235 Cidade Universitária, 50670-901 Recife, PE, Brazil.
authors.forename: Valéria
authors.identifier: 0000-0002-5751-7751
authors.initials: V
authors.lastname: Nunes-Souza
authors.affiliation: Pós-Graduação em Ciências da Saúde (PPGCS/UFAL), Maceió, AL, Brazil.
authors.forename: Juliana Célia de Farias
authors.initials: JC
authors.lastname: Santos
authors.affiliation: Pós-Graduação em Ciências da Saúde (PPGCS/UFAL), Maceió, AL, Brazil; Laboratório de Reatividade Cardiovascular (LRC/UFAL), Maceió, AL, Brazil; Rede Nordeste de Biotecnologia (RENORBIO), Recife, PE, Brazil.
authors.forename: Luiza Antas
authors.identifier: 0000-0001-9280-6527
authors.initials: LA
authors.lastname: Rabelo
authors.affiliation: Instituto de Química e Biotecnologia (IQB/UFAL), Maceió, AL, Brazil; Rede Nordeste de Biotecnologia (RENORBIO), Recife, PE, Brazil.
authors.forename: Marília Oliveira Fonseca
authors.identifier: 0000-0001-9860-3667
authors.initials: MO
authors.lastname: Goulart
mesh_terms: D000111:Acetylcysteine / Q000494:pharmacology / Q000627:therapeutic use*; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D003093:Colitis, Ulcerative / Q000139:chemically induced* / Q000188:drug therapy* / Q000473:pathology; D003106:Colon / Q000187:drug effects / Q000473:pathology; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D019587:Dietary Supplements; D005247:Feeding Behavior / Q000187:drug effects; D007249:Inflammation / Q000473:pathology; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009929:Organ Size / Q000187:drug effects; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017208:Rats, Wistar; D008063:Thioctic Acid / Q000494:pharmacology / Q000627:therapeutic use*
chemical_list: D016207:Cytokines; D008063:Thioctic Acid; D016264:Dextran Sulfate; D000111:Acetylcysteine