title: Circular RNA Expression Profile of Pancreatic Ductal Adenocarcinoma Revealed by Microarray.
abstract: OBJECTIVE
  Circular RNAs (circRNAs) are a special novel type of a stable, diverse and conserved noncoding RNA in mammalian cells. Particularly in cancer, circRNAs have been reported to be widely involved in the physiological/pathological process of life. However, it is unclear whether circRNAs are specifically involved in pancreatic ductal adenocarcinoma (PDAC).
  
  
  METHODS
  We investigated the expression profile of circRNAs in six PDAC cancer samples and paired adjacent normal tissues using microarray. A high-throughput circRNA microarray was used to identify dysregulated circular RNAs in six PDAC patients. Bioinformatic analyses were applied to study these differentially expressed circRNAs. Furthermore, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results.
  
  
  RESULTS
  We revealed and confirmed that a number of circRNAs were dysregulated, which suggests a potential role in pancreatic cancer.
  
  
  CONCLUSIONS
  this study demonstrates that clusters of circRNAs are aberrantly expressed in PDAC compared with normal samples and provides new potential targets for the future treatment of PDAC and novel insights into PDAC biology.
authors.affiliation: Department of Hepatobiliary Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
authors.forename: Haimin
authors.initials: H
authors.lastname: Li
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Hao
authors.forename: Huimin
authors.initials: H
authors.lastname: Wang
authors.forename: Zhengcai
authors.initials: Z
authors.lastname: Liu
authors.forename: Yong
authors.initials: Y
authors.lastname: He
authors.forename: Meng
authors.initials: M
authors.lastname: Pu
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.forename: Hengchao
authors.initials: H
authors.lastname: Yu
authors.forename: Juanli
authors.initials: J
authors.lastname: Duan
authors.forename: Shibin
authors.initials: S
authors.lastname: Qu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics*; D000328:Adult; D000368:Aged; D001483:Base Sequence; D021441:Carcinoma, Pancreatic Ductal / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D058977:Molecular Sequence Annotation; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D010190:Pancreatic Neoplasms / Q000235:genetics*; D012313:RNA / Q000235:genetics* / Q000378:metabolism; D000079962:RNA, Circular; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D015398:Signal Transduction / Q000235:genetics
chemical_list: D035683:MicroRNAs; D000079962:RNA, Circular; D012333:RNA, Messenger; D012313:RNA