title: Tunable Resistive Pulse Sensing for the Characterization of Extracellular Vesicles.
abstract: Accurate characterization of extracellular vesicles (EVs), including exosomes and microvesicles, is essential to obtain further knowledge on the biological relevance of EVs. Tunable resistive pulse sensing (tRPS) has shown promise as a method for single particle-based quantification and size profiling of EVs. Here, we describe the technical background of tRPS and its applications for EV characterization. Besides the standard protocol, we describe an alternative protocol, in which samples are spiked with polystyrene beads of known size and concentration. This alternative protocol can be used to overcome some of the challenges of direct EV characterization in biological fluids.
authors.affiliation: Department of Neurosurgery, University Medical Center Utrecht, Utrecht, The Netherlands.|Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Sybren L N
authors.initials: SL
authors.lastname: Maas
authors.affiliation: Department of Neurosurgery, University Medical Center Utrecht, Utrecht, The Netherlands.|Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Marike L D
authors.initials: ML
authors.lastname: Broekman
authors.affiliation: Department of Neurosurgery, University Medical Center Utrecht, Utrecht, The Netherlands. j.devrij@erasmusmc.nl.|Brain Center Rudolf Magnus, University Medical Center Utrecht, Utrecht, The Netherlands. j.devrij@erasmusmc.nl.|Department of Neurosurgery, Erasmus Medical Center, 3000 CA, Rotterdam, The Netherlands. j.devrij@erasmusmc.nl.
authors.forename: Jeroen
authors.initials: J
authors.lastname: de Vrij
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D055252:Cell-Derived Microparticles; D055354:Exosomes; D000067128:Extracellular Vesicles / Q000737:chemistry / Q000378:metabolism; D010316:Particle Size
keywords: Characterization; Exosomes; Extracellular vesicles; Microvesicles; Quantification; Resistive pulse sensing; Size distribution; qNano