title: Schisandrin B inhibits the proliferation and invasion of glioma cells by regulating the HOTAIR-micoRNA-125a-mTOR pathway.
abstract: Glioma is one of the most common malignant central nervous system tumors in humans. Schisandrin B (Sch B) has been confirmed to cause the proliferation and invasion of glioma cells. In the present study, the potential mechanism underlying the antitumor effect of Sch B on glioma cells was investigated. The glioma cell lines, U251 and U87, were exposed to Sch B, and the cell viability, apoptosis, migration, and invasion were determined using the MTT assay, flow cytometry, and transwell assay, respectively. Then, the effects of HOTAIR and miR-125a on tumor biology and the mammalian target of rapamycin (mTOR) protein expression in cell lines exposed to Sch B were investigated. The results showed that Sch B decreased HOTAIR expression and increased miR-125a-5p expression. HOTAIR overexpression decreased miR-125a expression and increased mTOR expression in cells with the treatment of Sch B. The miR-125a inhibitor reversed the effects of HOTAIR downregulation on cell proliferation and migration. On co-incubation with rapamycin, a specific mTOR inhibitor, the cell viability, migration, and invasion were decreased and cell apoptosis was increased in two cell lines exposed to Sch B after the treatment of pcDNA-HOTAIR. In conclusion, Sch B played an inhibitory role in the proliferation and invasion of glioma cells by regulating the HOTAIR-micoRNA-125a-mTOR pathway.
authors.affiliation: Department of Neurosurgery, The Affiliated Hospital of Inner Mongolia National University, Tongliao, Inner Mongolia, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jiang
authors.forename: Qiuli
authors.initials: Q
authors.lastname: Zhang
authors.forename: Jinsuo
authors.initials: J
authors.lastname: Bao
authors.forename: Chenghua
authors.initials: C
authors.lastname: Du
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.forename: Qiang
authors.initials: Q
authors.lastname: Tong
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
mesh_terms: D000704:Analysis of Variance; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D002452:Cell Count; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects* / Q000502:physiology; D034242:Cyclooctanes / Q000494:pharmacology; D005434:Flow Cytometry; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D005910:Glioma / Q000378:metabolism*; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D009361:Neoplasm Invasiveness / Q000503:physiopathology; D011083:Polycyclic Compounds / Q000494:pharmacology*; D062085:RNA, Long Noncoding; D015398:Signal Transduction / Q000187:drug effects*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism; D014162:Transfection
chemical_list: D000893:Anti-Inflammatory Agents; D034242:Cyclooctanes; C561566:HOTAIR long untranslated RNA, human; D017705:Lignans; D035683:MicroRNAs; D011083:Polycyclic Compounds; D062085:RNA, Long Noncoding; C015499:schizandrin B; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases