title: Aptamer-PEG-modified Fe3O4@Mn as a novel T1- and T2- dual-model MRI contrast agent targeting hypoxia-induced cancer stem cells.
abstract: Hypoxia-induced cancer stem cells have been known to be involved in tumour metastasis, resistance to chemo/radio therapy and tumour recurrence. Magnetic Resonance Imaging is a widely used imaging tool for cancers in clinics and research. To develop T1-positive and T2-negative dual mode MRI agents for more comprehensive and accurate diagnostic information under hypoxic conditions, a hypoxia-inducible factor-1α based aptamer and Mn(II)-modified nanoparticles D-Fe3O4@PMn were synthesized and characterized. In vitro and in vivo studies show that D-Fe3O4@PMn NPs are biocompatible and less cytotoxic and can produce significant contrast enhancement in T1- and T2-weighted MR imaging. Furthermore, the D-Fe3O4@PMn NPs enable targeted dual-contrast T1- and T2-weighted MR imaging of cancer cells expressing high levels of HIF-1α and cancer stem cell-related proteins under hypoxic condition. In conclusion, NPs with HIF-1α and Mn(II) are promising diagnostic agents for dual-mode T1 and T2 imaging by targeting cancer stem cells as they are non-toxic and biocompatible.
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.
authors.forename: Haitao
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Central Lab, Affiliated People's Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212002, China.
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.
authors.forename: Yuepeng
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Xie
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.
authors.forename: Lian
authors.initials: L
authors.lastname: Song
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.
authors.forename: Dongqing
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.|Turku PET Centre, Turku University Hospital and University of Turku, Turku, Finland.
authors.forename: Chunlei
authors.initials: C
authors.lastname: Han
authors.affiliation: Department of Radiology, Affiliated Hospital of JiangSu University, Zhengjiang, Jiangsu Province, 212001, China.|School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, Jiangsu Province, 212013, China.
authors.forename: Qiuyun
authors.initials: Q
authors.lastname: Chen
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000737:chemistry; D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D007668:Kidney / Q000473:pathology; D008099:Liver / Q000473:pathology; D008279:Magnetic Resonance Imaging; D008345:Manganese / Q000737:chemistry; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000000981:diagnostic imaging / Q000473:pathology; D014411:Neoplastic Stem Cells / Q000166:cytology / Q000378:metabolism; D011092:Polyethylene Glycols / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D003287:Contrast Media; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D011092:Polyethylene Glycols; D008345:Manganese; D052203:Ferrosoferric Oxide