title: ZFP91: A Noncanonical NF-κB Signaling Pathway Regulator with Oncogenic Properties Is Overexpressed in Prostate Cancer.
abstract: Novel molecular targets are being searched to aid in prostate cancer diagnosis and therapy. Recently, ZFP91 zinc finger protein has been found to be upregulated in prostate cancer cell lines. It is a potentially important oncogenic protein; however only limited data regarding its biological function and expression patterns are available. To date, ZFP91 has been shown to be a key factor in activation of noncanonical NF-κB signaling pathway as well as to be involved in HIF-1αsignaling in cancer cells. The present study aimed to characterizeZFP91expression in prostate cancer specimens. Furthermore, since our earlier reports showed discrepancies betweenZFP91mRNA and protein levels, we studied this interrelationship in LNCaP and PC-3 prostate cancer cell lines using siRNA mediated knockdown. QPCR analysis revealed marked upregulation of ZFP91 mRNA in the majority of prostate cancer specimens. Transfection of prostate cancer cells withZFP91siRNA resulted in a 10-fold decrease in mRNA levels. On a protein level, however, no inhibitory effect was observed over the time of the cell culture. We conclude thatZFP91is overexpressed in prostate cancer and that potential accumulation of the ZFP91 protein in studied cells may be of importance in prostate cancer biology.
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781 Poznan, Poland.
authors.forename: Lukasz
authors.identifier: 0000-0002-9481-7876
authors.initials: L
authors.lastname: Paschke
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781 Poznan, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Jopek
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781 Poznan, Poland.
authors.forename: Marta
authors.identifier: 0000-0003-0150-3665
authors.initials: M
authors.lastname: Szyszka
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781 Poznan, Poland.
authors.forename: Marianna
authors.identifier: 0000-0003-0623-9496
authors.initials: M
authors.lastname: Tyczewska
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781 Poznan, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Ziolkowska
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781 Poznan, Poland.
authors.forename: Marcin
authors.identifier: 0000-0002-2525-5777
authors.initials: M
authors.lastname: Rucinski
authors.affiliation: Department of Histology and Embryology, Poznan University of Medical Sciences, 6 Swiecicki St., 60-781 Poznan, Poland.
authors.forename: Ludwik K
authors.initials: LK
authors.lastname: Malendowicz
mesh_terms: D045744:Cell Line, Tumor; D004587:Electrophoresis, Agar Gel; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006801:Humans; D015151:Immunoblotting; D008297:Male; D016328:NF-kappa B / Q000378:metabolism*; D060787:Neoplasm Grading; D009857:Oncogenes; D011471:Prostatic Neoplasms / Q000235:genetics*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism; D015398:Signal Transduction; D014162:Transfection; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*
chemical_list: D016328:NF-kappa B; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D044767:Ubiquitin-Protein Ligases; C553044:ZFP91 protein, human