title: Improvement stability and performance of invertase via immobilization on to silanized and polymer brush grafted magnetic nanoparticles.
abstract: In this study, magnetic nanoparticles (Fe3O4) were modified sequentially with silica (Fe3O4@SiO2), glycidyl methacrylate (GMA) by surface initiated atom transfer radical polymerization (SI-ATRP) and hexamethylene diamine (as a spacer arm). The p(GMA) grafted and SA modified form (i.e., Fe3O4@SiO2@pGMA-SA-3) was used for covalent immobilization of invertase (EC 3.2.1.26). The amount of immobilized enzyme on Fe3O4@SiO2@p(GMA) and Fe3O4@SiO2@p(GMA)-SA-3 was 36.1±0.9 and 33.4±1.3mg/g, respectively. The Kmand Vmaxvalues of immobilized invertase were found to be 39.4mmol/L and 349.5mmol/L min, and not significantly changed compared with free form (34.3mmol/L and 387.2mmol/Lmin), respectively, revealed that the applied protocol did not have any detrimental effect on the retained activity of immobilized invertase.
authors.affiliation: Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Ankara, Turkey; Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Ankara, Turkey. Electronic address: g_bayramoglu@hotmail.com.
authors.forename: Gulay
authors.initials: G
authors.lastname: Bayramoglu
authors.affiliation: Department of Chemistry, Faculty of Sciences, Gazi University, 06500 Ankara, Turkey.
authors.forename: Tugce
authors.initials: T
authors.lastname: Doz
authors.affiliation: Istanbul Kemerburgaz University, School of Medicine, 34217 Istanbul, Turkey.
authors.forename: V Cengiz
authors.initials: VC
authors.lastname: Ozalp
authors.affiliation: Biochemical Processing and Biomaterial Research Laboratory, Gazi University, 06500 Ankara, Turkey.
authors.forename: M Yakup
authors.initials: MY
authors.lastname: Arica
mesh_terms: D017438:Bisphenol A-Glycidyl Methacrylate / Q000737:chemistry*; D008280:Magnetics; D053758:Nanoparticles; D011108:Polymers; D043324:beta-Fructofuranosidase / Q000737:chemistry*
chemical_list: D011108:Polymers; D017438:Bisphenol A-Glycidyl Methacrylate; C033930:Silar (3M); D043324:beta-Fructofuranosidase
keywords: Continuous system; Immobilization; Invertase; Kinetic parameter; Magnetic support; Sucrose hydrolysis