title: Electrochemical H2O2biosensor composed of myoglobin on MoS2nanoparticle-graphene oxide hybrid structure.
abstract: In this research, the electrochemical biosensor composed of myoglobin (Mb) on molybdenum disulfide nanoparticles (MoS2NP) encapsulated with graphene oxide (GO) was fabricated for the detection of hydrogen peroxide (H2O2). Hybrid structure composed of MoS2NP and GO (GO@MoS2) was fabricated for the first time to enhance the electrochemical signal of the biosensor. As a sensing material, Mb was introduced to fabricate the biosensor for H2O2detection. Formation and immobilization of GO@MoS2was confirmed by transmission electron microscopy, ultraviolet-visible spectroscopy, scanning electron microscopy, and scanning tunneling microscopy. Immobilization of Mb, and electrochemical property of biosensor were investigated by cyclic voltammetry and amperometric i-t measurements. Fabricated biosensor showed the electrochemical signal enhanced redox current as -1.86μA at an oxidation potential and 1.95μA at a reduction potential that were enhanced relative to those of electrode prepared without GO@MoS2. Also, this biosensor showed the reproducibility of electrochemical signal, and retained the property until 9 days from fabrication. Upon addition of H2O2, the biosensor showed enhanced amperometric response current with selectivity relative to that of the biosensor prepared without GO@MoS2. This novel hybrid material-based biosensor can suggest a milestone in the development of a highly sensitive detecting platform for biosensor fabrication with highly sensitive detection of target molecules other than H2O2.
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea.
authors.forename: Jinho
authors.initials: J
authors.lastname: Yoon
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea; Institute of Integrated Biotechnology, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea.
authors.forename: Taek
authors.initials: T
authors.lastname: Lee
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea.
authors.forename: Bharate
authors.initials: B
authors.lastname: Bapurao G
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea.
authors.forename: Jinhee
authors.initials: J
authors.lastname: Jo
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea.
authors.forename: Byung-Keun
authors.initials: BK
authors.lastname: Oh
authors.affiliation: Department of Chemical & Biomolecular Engineering, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea; Institute of Integrated Biotechnology, Sogang University, 35 Baekbeom-Ro, Mapo-Gu, Seoul 121-742, South Korea. Electronic address: jwchoi@sogang.ac.kr.
authors.forename: Jeong-Woo
authors.initials: JW
authors.lastname: Choi
mesh_terms: D015374:Biosensing Techniques; D055664:Electrochemical Techniques; D006861:Hydrogen Peroxide / Q000737:chemistry / Q000302:isolation & purification*; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D008982:Molybdenum / Q000737:chemistry*; D009211:Myoglobin / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry; D010087:Oxides / Q000737:chemistry*
chemical_list: D009211:Myoglobin; D010087:Oxides; C539565:molybdenum dioxide; D008982:Molybdenum; D006861:Hydrogen Peroxide
keywords: Biosensor; Electrochemical signal enhancement; Graphene oxide; Hydrogen peroxide; MoS(2) nanoparticle; Myoglobin