title: Contribution of Persistent Organic Pollutant Exposure to the Adipose Tissue Oxidative Microenvironment in an Adult Cohort: A Multipollutant Approach.
abstract: Despite growing in vitro and in vivo evidence of the putative role of persistent organic pollutants (POPs) in the induction of oxidative damage in cell structures, this issue has been poorly addressed from an epidemiologic perspective. The aim of this study was to explore associations between adipose tissue POP concentrations and the in situ oxidative microenvironment. A cross-sectional study was conducted in a subsample (n = 271) of a previously established cohort, quantifying levels of eight POPs and four groups of oxidative stress biomarkers in adipose tissue. Associations were explored using multivariate linear regression analyses adjusted for potential confounders. We assessed the combined effect of POPs on oxidative stress/glutathione system biomarkers using weighted quantile sum regression (WQS). Increased concentrations of p,p'-DDE, HCB, β-HCH, dicofol, and PCBs (congeners -138, -153, and -180) were predominantly associated with higher lipid peroxidation (TBARS) [exp(β) = 1.09-1.78, p < 0.01-0.04)] and SOD activity [exp(β) = 1.13-1.48, p < 0.01-0.05)] levels. However, only a few associations were observed with glutathione system biomarkers, e.g., PCB-180 with total glutathione [exp(β) = 1.98, p = 0.03]. The WQS index was found to be positively associated with SOD activity, and PCB-138, PCB-180, and β-HCH were the main contributors to the index. Likewise, the WQS index was positively associated with TBARS levels, with the three PCBs acting as the main contributors. This is the first epidemiological evidence of the putative disruption by POPs of the adipose tissue oxidative microenvironment. Our results indicate that POP exposure may enhance alternative pathways to the glutathione detoxification route, which might result in tissue damage. Further research is warranted to fully elucidate the potential health implications.
authors.affiliation: Radiology and Physical Medicine Department, University of Granada , Granada, 18012, Spain.|Instituto de Investigación Biosanitaria (ibs.GRANADA), Hospitales Universitarios de Granada , Granada, 18012, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Artacho-Cordón
authors.affiliation: Instituto de Investigación Biosanitaria (ibs.GRANADA), Hospitales Universitarios de Granada , Granada, 18012, Spain.|CIBER en Enfermedades Hepáticas y Digestivas (CIBEREHD) , 28029 Madrid, Spain.
authors.forename: Josefa
authors.initials: J
authors.lastname: León
authors.affiliation: Instituto de Investigación Biosanitaria (ibs.GRANADA), Hospitales Universitarios de Granada , Granada, 18012, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Sáenz
authors.affiliation: Radiology and Physical Medicine Department, University of Granada , Granada, 18012, Spain.|Instituto de Investigación Biosanitaria (ibs.GRANADA), Hospitales Universitarios de Granada , Granada, 18012, Spain.|CIBER en Epidemiología y Salud Pública (CIBERESP) , 28029 Madrid, Spain.
authors.forename: Mariana F
authors.initials: MF
authors.lastname: Fernández
authors.affiliation: Escuela Andaluza de Salud Pública , Granada, 18011, Spain.
authors.forename: Piedad
authors.initials: P
authors.lastname: Martin-Olmedo
authors.affiliation: Radiology and Physical Medicine Department, University of Granada , Granada, 18012, Spain.|Instituto de Investigación Biosanitaria (ibs.GRANADA), Hospitales Universitarios de Granada , Granada, 18012, Spain.|CIBER en Epidemiología y Salud Pública (CIBERESP) , 28029 Madrid, Spain.
authors.forename: Nicolás
authors.initials: N
authors.lastname: Olea
authors.affiliation: Instituto de Investigación Biosanitaria (ibs.GRANADA), Hospitales Universitarios de Granada , Granada, 18012, Spain.|Escuela Andaluza de Salud Pública , Granada, 18011, Spain.|Oncology Unit, Virgen de las Nieves University Hospital , Granada, 18012 Spain.
authors.forename: Juan P
authors.identifier: 0000-0002-8643-2423
authors.initials: JP
authors.lastname: Arrebola
mesh_terms: D000273:Adipose Tissue; D000328:Adult; D003430:Cross-Sectional Studies; D003633:Dichlorodiphenyl Dichloroethylene; D004785:Environmental Pollutants; D005978:Glutathione; D006801:Humans; D011078:Polychlorinated Biphenyls
chemical_list: D004785:Environmental Pollutants; D003633:Dichlorodiphenyl Dichloroethylene; D011078:Polychlorinated Biphenyls; D005978:Glutathione