title: Salting-out assisted liquid-liquid extraction coupled to ultra-high performance liquid chromatography-tandem mass spectrometry for the determination of tetracycline residues in infant foods.
abstract: The use of salting-out assisted liquid-liquid extraction (SALLE) combined with ultra-high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) has been evaluated for the determination of tetracyclines in infant foods based on meat and vegetables or in milk. To obtain satisfactory extraction efficiencies for the studied analytes, several parameters affecting the SALLE procedure were optimized. Analytical performances of the method were satisfactory, obtaining limits of quantification lower than 0.48μgkg-1in all cases. The precision, expressed as relative standard deviation (%, RSD) was below 11.3%. The extraction efficiency for fortified samples ranged from 89.2 to 96.8%, with RSDs lower than 7.3%. Matrix effect was evaluated for all samples studied, being lower than |21|% in all cases. In relation to the low solvent consumption, the proposed methodology could be considered rapid, cheap and environmentally friendly. Its applicability has been successfully tested in a wide range of infant foods.
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Av. Fuentenueva S/N, 18071 Granada, Spain.
authors.forename: David
authors.initials: D
authors.lastname: Moreno-González
authors.affiliation: Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Av. Fuentenueva S/N, 18071 Granada, Spain. Electronic address: amgarcia@ugr.es.
authors.forename: Ana M
authors.initials: AM
authors.lastname: García-Campaña
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005504:Food Analysis / Q000379:methods*; D006801:Humans; D007223:Infant; D007225:Infant Food / Q000032:analysis*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D008460:Meat / Q000032:analysis; D008892:Milk / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013754:Tetracyclines / Q000032:analysis*; D014675:Vegetables / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D013754:Tetracyclines
keywords: Antibiotics; Infant foods; Salting-out assisted liquid–liquid extraction; Tetracyclines; Ultra-high performance liquid chromatography tandem mass spectrometry