title: High-throughput sequencing analysis reveals the genetic diversity of different regions of the murine norovirus genome during in vitro replication.
abstract: In this study, we report the genetic diversity and nucleotide mutation rates of five representative regions of the murine norovirus genome during in vitro passages. The mutation rates were similar in genomic regions encompassing partial coding sequences for non-structural (NS) 1-2, NS5, NS6, NS7 proteins within open reading frame (ORF) 1. In a region encoding a portion of the major capsid protein (VP1) within ORF2 (also including the ORF4 region) and a portion of the minor structural protein (VP2), the mutation rates were estimated to be at least one order of magnitude higher. The VP2 coding region was found to have the highest mutation rate.
authors.affiliation: Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research for Animal & Health Centre, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium. amauroy@ulg.ac.be.
authors.forename: Axel
authors.initials: A
authors.lastname: Mauroy
authors.affiliation: Food Microbiology, Fundamental and Applied Research for Animal & Health Centre, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Bernard
authors.initials: B
authors.lastname: Taminiau
authors.affiliation: Quality Partner S.A., Herstal, Belgium.
authors.forename: Carine
authors.initials: C
authors.lastname: Nezer
authors.affiliation: Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research for Animal & Health Centre, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Elsa
authors.initials: E
authors.lastname: Ghurburrun
authors.affiliation: Eukaryotic Phylogenomics, Department of Life Sciences, University of Liège, Liège, Belgium.
authors.forename: Denis
authors.initials: D
authors.lastname: Baurain
authors.affiliation: Food Microbiology, Fundamental and Applied Research for Animal & Health Centre, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Georges
authors.initials: G
authors.lastname: Daube
authors.affiliation: Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research for Animal & Health Centre, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: Etienne
authors.initials: E
authors.lastname: Thiry
mesh_terms: D000818:Animals; D017250:Caliciviridae Infections / Q000662:veterinary* / Q000821:virology; D004261:DNA Replication; D014644:Genetic Variation; D016679:Genome, Viral; D059014:High-Throughput Nucleotide Sequencing; D051379:Mice; D008969:Molecular Sequence Data; D009154:Mutation; D029322:Norovirus / Q000235:genetics* / Q000302:isolation & purification / Q000502:physiology; D016366:Open Reading Frames; D012376:Rodent Diseases / Q000821:virology*; D012692:Serial Passage; D017361:Viral Nonstructural Proteins / Q000235:genetics* / Q000378:metabolism; D014779:Virus Replication
chemical_list: D017361:Viral Nonstructural Proteins
keywords: Genetic diversity; Murine; Mutation; Next-generation sequencing; Norovirus