title: Hyperactive mutant of a wheat plasma membrane Na+/H+antiporter improves the growth and salt tolerance of transgenic tobacco.
abstract: Wheat SOS1 (TaSOS1) activity could be relieved upon deletion of the C-terminal 168 residues (the auto-inhibitory domain). This truncated form of wheat SOS1 (TaSOS1-974) was shown to increase compensation (compared to wild-type TaSOS1) for the salt sensitivity of a yeast mutant strain, AXT3K, via increased Na+transportation out of cells during salinity stress. Expression of the plasma membrane proteins TaSOS1-974 or TaSOS1 improved the growth of transgenic tobacco plants compared with wild-type plants under normal conditions. However, plants expressing TaSOS1-974 grew better than TaSOS1-transformed plants. Upon salinity stress, Na+efflux and K+influx rates in the roots of transgenic plants expressing TaSOS1-974 or TaSOS1 were greater than those of wild-type plants. Furthermore, compared to TaSOS1-transgenic plants, TaSOS1-974-expressing roots showed faster Na+efflux and K+influx, resulting in less Na+and more K+accumulation in TaSOS1-974-transgenic plants compared to TaSOS1-transgenic and wild-type plants. TaSOS1-974-expressing plants had the lowest MDA content and electrolyte leakage among all tested plants, indicating that TaSOS1-974 might protect the plasma membrane against oxidative damage generated by salt stress. Overall, TaSOS1-974 conferred higher salt tolerance in transgenic plants compared to TaSOS1. Consistent with this result, transgenic plants expressing TaSOS1-974 showed a better growth performance than TaSOS1-expressing and wild-type plants under saline conditions.
authors.affiliation: National Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: National Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Zesen
authors.initials: Z
authors.lastname: Lai
authors.affiliation: National Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Yin
authors.affiliation: National Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Yu
authors.affiliation: National Key Laboratory of Wheat and Maize Crop Science/College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xu
authors.affiliation: National Key Laboratory of Wheat and Maize Crop Science/College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: National Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Xinli
authors.initials: X
authors.lastname: Cong
authors.affiliation: National Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agriculture, Hainan University, Haikou 570228, China.
authors.forename: Yuehua
authors.initials: Y
authors.lastname: Luo
authors.affiliation: National Key Laboratory of Wheat and Maize Crop Science/College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China. Electronic address: hauxhx@163.com.
authors.forename: Haixia
authors.initials: H
authors.lastname: Xu
authors.affiliation: National Key Laboratory for Sustainable Utilization of Tropical Bioresources/College of Agriculture, Hainan University, Haikou 570228, China. Electronic address: jiangxingyuhu@163.com.
authors.forename: Xingyu
authors.initials: X
authors.lastname: Jiang
mesh_terms: D008315:Malondialdehyde / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D030821:Plants, Genetically Modified / Q000254:growth & development / Q000378:metabolism*; D011188:Potassium / Q000378:metabolism; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D017923:Sodium-Hydrogen Exchangers / Q000235:genetics* / Q000378:metabolism; D014026:Nicotiana / Q000254:growth & development / Q000378:metabolism*; D014908:Triticum / Q000235:genetics*
chemical_list: D017923:Sodium-Hydrogen Exchangers; D008315:Malondialdehyde; D011188:Potassium
keywords: Auto-inhibitory domain; Plasma membrane Na(+)/H(+) antiporter; Salt tolerance; Transgenic tobacco; Wheat