title: Knockdown of ST6Gal-I increases cisplatin sensitivity in cervical cancer cells.
abstract: Sialyltransferase I (ST6Gal-I) is an enzyme involved in tumor metastasis that processes sialic acid precursors into their mature form, enabling them to regulate gene expression. However, the effect of ST6Gal-I on the biological behavior of cancer cells remain unclear. This study was the first to demonstrate the influence of ST6Gal-I on cisplatin sensitivity in cervical cancer cells.
  
  
  
  Knockdown of ST6Gal-I was performed by shRNA and HeLa cells combination with cisplatin were tested.
  
  
  
  We showed that down-regulation of ST6Gal-I promoted cell apoptosis and inhibited proliferation and invasion in cervical cancer cells. Knockdown of ST6Gal-I by RNA interference increased the sensitivity of HeLa cells to cisplatin in vitro, and reduced tumor volume and suppressed subcutaneous tumor growth in response to cisplatin treatment in a xenograft mouse model in vivo.
  
  
  
  The results provide new information that ST6Gal-I plays an important role in several biological or pathological processes including drug resistance in cervical cancer and may be a potential therapeutic target to improve the response to chemotherapy in cervical cancer patients.
authors.affiliation: Department of Obstetrics and Gynecology, The First Affiliated Hospital of Anhui Medical University, Hefei, 23022, China.
authors.forename: Xiaopeng
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, Anhui Provincial Hospital, Anhui Medical University, Hefei, 23000, China.
authors.forename: Chunchen
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Pathology, First Affiliated Hospital, Bengbu Medical College, 287 Changhuai Road, Bengbu, 233000, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Department of Pathology, First Affiliated Hospital, Bengbu Medical College, 287 Changhuai Road, Bengbu, 233000, China.
authors.forename: Zhaogen
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Department of Pathology, First Affiliated Hospital, Bengbu Medical College, 287 Changhuai Road, Bengbu, 233000, China.
authors.forename: Shufang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Pathology, First Affiliated Hospital, Bengbu Medical College, 287 Changhuai Road, Bengbu, 233000, China. ydh_2210@126.com.
authors.forename: Donghong
authors.initials: D
authors.lastname: Yu
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000008:administration & dosage* / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D004357:Drug Synergism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D055785:Gene Knockdown Techniques; D006367:HeLa Cells; D006801:Humans; D051379:Mice; D034741:RNA, Small Interfering / Q000494:pharmacology*; D012799:Sialyltransferases / Q000235:genetics*; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000235:genetics; D023041:Xenograft Model Antitumor Assays; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
chemical_list: D015703:Antigens, CD; D002945:Cisplatin; D034741:RNA, Small Interfering; D012799:Sialyltransferases; C093493:ST6GAL1 protein, human; D000094802:beta-D-Galactoside alpha 2-6-Sialyltransferase
keywords: Apoptosis; DDP; HeLa; Invasion; α-2,6-sialic acid transferase