title: Analysis of Serum Cholesterol Efflux Capacity in a Minipig Model of Nonischemic Heart Failure.
abstract: OBJECTIVE
  Circulating levels of high-density lipoprotein cholesterol (HDL-C) are decreased in patients with heart failure (HF). We tested whether HDL-C serum levels are associated with cardiac contractile dysfunction in a minipig HF model.
  
  
  METHODS
  Blood samples were collected from 13 adult male minipigs: 1) before pacemaker implantation, 2) 10 days after surgery, and 3) 3 weeks after high-rate LV pacing. Serum cholesterol efflux capacity (CEC), an index of HDL functionality, was assessed through four mechanisms: ATP Binding Cassette transporter A1 (ABCA1), ATP Binding Cassette transporter G1 (ABCG1), Scavenger Receptor-Class B Type I (SR-BI) and Passive Diffusion (PD).
  
  
  RESULTS
  HDL-C serum levels significantly decrease in minipigs with HF compared with baseline (p＜0.0001). Serum CEC mediated by PD and SR-BI, but not ABCA1 or ABCG1, significantly decrease in animals with HF (p＜0.05 and p＜0.005, respectively).
  
  
  CONCLUSIONS
  HDL-C serum levels and partial serum CEC reduction may play a pathophysiological role in the cardiac function decay sustained by high-rate LV pacing, opening new avenues to understand of the pathogenesis of nonischemic myocardial remodeling.
authors.affiliation: Fondazione Toscana Gabriele Monasterio.
authors.forename: Federico
authors.initials: F
authors.lastname: Bigazzi
authors.affiliation: Department of Pharmacy, University of Parma.
authors.forename: Maria Pia
authors.initials: MP
authors.lastname: Adorni
authors.affiliation: CNR Institute of Clinical Physiology.
authors.forename: Mariarita
authors.initials: M
authors.lastname: Puntoni
authors.affiliation: Fondazione Toscana Gabriele Monasterio.
authors.forename: Francesco
authors.initials: F
authors.lastname: Sbrana
authors.affiliation: Fondazione Toscana Gabriele Monasterio.|Laboratory of Medical Science, Institute of Life Sciences, Scuola Superiore Sant'Anna.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Lionetti
authors.affiliation: Fondazione Toscana Gabriele Monasterio.
authors.forename: Beatrice Dal
authors.initials: BD
authors.lastname: Pino
authors.affiliation: Department of Pharmacy, University of Parma.
authors.forename: Elda
authors.initials: E
authors.lastname: Favari
authors.affiliation: Laboratory of Medical Science, Institute of Life Sciences, Scuola Superiore Sant'Anna.|Department of Physiology, Temple University School of Medicine.
authors.forename: Fabio A
authors.initials: FA
authors.lastname: Recchia
authors.affiliation: Department of Pharmacy, University of Parma.
authors.forename: Franco
authors.initials: F
authors.lastname: Bernini
authors.affiliation: Fondazione Toscana Gabriele Monasterio.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Sampietro
mesh_terms: D064286:ATP Binding Cassette Transporter 1 / Q000097:blood*; D000818:Animals; D001692:Biological Transport; D015415:Biomarkers / Q000097:blood*; D008076:Cholesterol, HDL / Q000097:blood*; D004195:Disease Models, Animal; D006333:Heart Failure / Q000097:blood* / Q000473:pathology*; D008297:Male; D013552:Swine; D013556:Swine, Miniature
chemical_list: D064286:ATP Binding Cassette Transporter 1; D015415:Biomarkers; D008076:Cholesterol, HDL
keywords: Cholesterol efflux; HDL; Minipig; Non-ischemic heart failure