title: Zein/Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) electrospun blend fiber scaffolds: Preparation, characterization and cytocompatibility.
abstract: In the present work, a series of Zein/Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) blend fiber scaffolds have been prepared by electrospinning method. The electrospun fibers showed a circular and uniform morphology with random distribution. The blend fiber scaffolds possessed well interconnected porous fibrous network structure with high porosity and large aspect surface areas. The FTIR and XPS spectra of Zein/P(3HB-co-4HB) blend fibers demonstrated the same characteristics to that of pure Zein and P(3HB-co-4HB) electrospun fibers. However, Zein might hinder the crystallization of P(3HB-co-4HB) owing to the formation of weak intermolecular interactions, which can affect the preferential orientation of P(3HB-co-4HB) molecules. Only one glass transition temperature (Tg) can be detected for electrospun Zein/P(3HB-co-4HB) blend fiber scaffolds implying the miscibility of Zein and P(3HB-co-4HB) in the blend fibers. The Zein/P(3HB-co-4HB) blend fiber scaffolds showed about 50% of improvement in tensile strength and 400% of increase in elongation at break by increasing P(3HB-co-4HB) content from 20% to 80%. The cytocompatibility of the Zein/P(3HB-co-4HB) blend fiber scaffolds was preliminarily evaluated by cell culture in vitro. The as-prepared electrospun Zein/P(3HB-co-4HB) blend fiber scaffolds with the characteristics of good biocompatibility, excellent pore characteristic as well as sufficient mechanical properties should be more promising for applications as tissue engineering scaffold.
authors.affiliation: School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China; State Key Laboratory of Hollow Fiber Membrane Material and Processes, No 399 BingShuiXi Street, Tianjin 300387, XiQing District, China. Electronic address: caizhijiang@hotmail.com.
authors.forename: Cai
authors.initials: C
authors.lastname: Zhijiang
authors.affiliation: School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Qin
authors.affiliation: School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Song
authors.initials: S
authors.lastname: Xianyou
authors.affiliation: School of Textiles, Tianjin Polytechnic University, Tianjin 300387, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yuanpei
mesh_terms: D000818:Animals; D002152:Calorimetry, Differential Scanning; D016923:Cell Death / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D006885:Hydroxybutyrates / Q000494:pharmacology*; D051379:Mice; D041681:NIH 3T3 Cells; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects; D056951:Photoelectron Spectroscopy; D011091:Polyesters / Q000494:pharmacology*; D017550:Spectroscopy, Fourier Transform Infrared; D013314:Stress, Mechanical; D013499:Surface Properties; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014961:X-Ray Diffraction; D015028:Zein / Q000494:pharmacology*; D000094122:Polyhydroxybutyrates
chemical_list: D006885:Hydroxybutyrates; D011091:Polyesters; D015028:Zein; C068056:poly(3-hydroxybutyrate-co-4-hydroxybutyrate); D000094122:Polyhydroxybutyrates
keywords: Blend; Electrospinning; Poly(3-hydroxybutyrate-co-4-hydroxybutyrate); Scaffold; Zein