title: Bcl-2 overexpression contributes to laryngeal carcinoma cell survival by forming a complex with Hsp90β.
abstract: Laryngeal carcinoma (LC) is one of the most common malignant tumors of all head and neck squamous cell carcinomas (HNSCCs). However, the molecular mechanism and genetic basis of the development of LC have not been fully elucidated. To explore the possible mechanism, targeted proteomic analysis was performed on Bcl-2-associated proteins from LC cells. According to our results, 35 proteins associated with Bcl-2 were identified and Hsp90β was confirmed by co-immunoprecipitation and western blot analysis. Protein‑protein interaction (PPI) analysis indicated that Bcl-2‑Hsp90β interactions may be involved in the anti-apoptotic progression of LC. Further results revealed that disruption of the Bcl-2-Hsp90β interaction inhibited the anti-apoptotic ability of Bcl-2 and decreased the caspase activation in LC, which has broad implications for the better understanding of tumor formation, tumor cell survival and development of metastasis due to Bcl-2. Collectively, we report the mechanism by which Bcl-2 functions in LC as an anti-apoptotic factor in relation to its association with proteins and potentially identify a Bcl-2/Hsp90β axis as a novel target for LC therapy.
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Sai
authors.initials: S
authors.lastname: Li
authors.affiliation: Medical School, Shaoyang University, Shaoyang, Hunan 422000, P.R. China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Li
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Tian
authors.initials: T
authors.lastname: Hu
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Chuhong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Lv
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Sha
authors.initials: S
authors.lastname: He
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Hanxing
authors.initials: H
authors.lastname: Yan
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Yixi
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Wen
authors.affiliation: Department of Respiratory and Critical Care Medicine, The People's Hospital of Zhangjiajie City, Zhangjiajie, Hunan 427000, P.R. China.
authors.forename: Mingsheng
authors.initials: M
authors.lastname: Lei
authors.affiliation: Oncology Department, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan 421001, P.R. China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Zuo
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002294:Carcinoma, Squamous Cell / Q000378:metabolism / Q000556:secondary*; D049109:Cell Proliferation; D005260:Female; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D047468:Immunoprecipitation; D007822:Laryngeal Neoplasms / Q000378:metabolism / Q000473:pathology*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D040901:Proteomics; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D018841:HSP90 Heat-Shock Proteins; C493165:HSP90AB1 protein, human; D019253:Proto-Oncogene Proteins c-bcl-2