title: Synthesis of the light/pH responsive polymer for immobilization of α-amylase.
abstract: In this study, light/pH responsive methoxy poly (ethylene glycol)-(5-propargylether-2-nitrobenzyl bromoisobutyrate)-poly methylacrylic acid-b-polystyrene (mPEG-ONB-PMAA-b-PS) polymers were synthesized, and successfully utilized to fabricate micelles and immobilize α-amylase. The critical micelle concentrations (CMC) of the polymers were measured with Pyrene Fluorescent Probe Technique. The morphology and diameter of micelles were characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). In addition, the effects of pH, temperature and light-responsive on the catalytic activity were investigated. The optimized fabrication conditions of α-amylase-loaded micelles which α-amylase gave the higher activity were as follows: Immobilization time, 60min; Immobilization temperature, 50°C; enzyme concentration, 10UmL-1; PBS buffer, pH=5.4. α-Amylase immobilized in these micelles was much more stable than that free α-amylase.
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Material Science and Engineering, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhao
authors.affiliation: Basic Experimental Teaching Center, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China.
authors.forename: Kehu
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710119, China. Electronic address: lzl2016@snnu.edu.cn.
authors.forename: Zhongli
authors.initials: Z
authors.lastname: Lei
mesh_terms: D063566:Biodegradable Plastics / Q000138:chemical synthesis / Q000737:chemistry; D004800:Enzymes, Immobilized / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D008027:Light; D008823:Micelles; D011092:Polyethylene Glycols / Q000737:chemistry; D011109:Polymethacrylic Acids / Q000737:chemistry; D000516:alpha-Amylases / Q000737:chemistry*
chemical_list: D063566:Biodegradable Plastics; D004800:Enzymes, Immobilized; D008823:Micelles; D011109:Polymethacrylic Acids; C030613:polymethacrylic acid; D011092:Polyethylene Glycols; D000516:alpha-Amylases; C000595209:polyethylene glycol 1000
keywords: Immobilized enzyme; Light/pH-sensitive polymer; Smart polymer