title: A functional centromere lacking CentO sequences in a newly formed ring chromosome in rice.
abstract: An awned rice (Oryza sativa) plant carrying a tiny extra chromosome was discovered among the progeny of a telotrisomic line 2n+4L. Fluorescence in situ hybridization (FISH) using chromosome specific BAC clones revealed that this extra chromosome was a ring chromosome derived from part of the long arm of chromosome 4. So the aneuploidy plant was accordingly named as 2n+4L ring. We did not detect any CentO FISH signals on the ring chromosome, and found only the centromeric probe Centromeric Retrotransposon of Rice (CRR) was co-localized with the centromere-specific histone CENH3 as revealed by sequential FISH after immunodetection. The extra ring chromosome exhibited a unique segregation pattern during meiosis, including no pairing between the ring chromosome and normal chromosome 4 during prophase I and pre-separation of sister chromatids at anaphase I.
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yafei
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Su
authors.affiliation: State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Shen
authors.affiliation: State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Ding
authors.initials: D
authors.lastname: Tang
authors.affiliation: State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Ministry of Education Key Laboratory of Agriculture Biodiversity for Plant Disease Management, Yunnan Agricultural University, Kunming 650201, China. Electronic address: qiongbf@aliyun.com.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Plant Genomics, Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China. Electronic address: zkcheng@genetics.ac.cn.
authors.forename: Zhukuan
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D002503:Centromere / Q000235:genetics*; D008540:Meiosis / Q000235:genetics; D009154:Mutation; D012275:Oryza / Q000166:cytology / Q000235:genetics* / Q000502:physiology; D012098:Reproduction; D012303:Ring Chromosomes; D020080:Tandem Repeat Sequences
keywords: Centromere; Meiosis; Rice; Ring chromosome