title: Tumor-associated autoantibodies against Fascin as a novel diagnostic biomarker for esophageal squamous cell carcinoma.
abstract: OBJECTIVE
  Autoantibodies against tumor-associated antigens (TAAs) have been found in many kinds of cancers, and might serve as biomarkers for early cancer diagnosis. The present study was carried out to test if there is any relation between autoantibodies against Fascin and esophageal squamous cell carcinoma (ESCC).
  
  
  METHODS
  One hundred and forty-nine patients with ESCC and 98 control subjects were recruited in the study. The levels of circulating autoantibodies against Fascin were measured by enzyme-linked immunosorbent assay (ELISA). Receiver operating characteristic (ROC) was used to calculate diagnostic accuracy.
  
  
  RESULTS
  The levels of autoantibodies against Fascin in patients with ESCC were significantly higher than in control subjects (P<0.001). Measurement of autoantibodies against Fascin provided an area under the curve (AUC) of 0.636, [95% confidence interval (CI): 0.568-0.704] 24.8% sensitivity (95% CI: 18.3%-37.2%) and 99.0% specificity (95% CI: 93.6%-99.9%). Moreover, serum level of autoantibodies against Fascin in early-stage ESCC was significantly higher than that of normal controls (P<0.05). The positive rates of autoantibodies against Fascin were correlated with age (P<0.05), but not with gender, tumor size, tumor site, histological grade, T stage, N stage or TNM stage (P>0.05).
  
  
  CONCLUSIONS
  Our results suggest that Fascin autoantibody may be a potential biomarker for the early detection of ESCC.
authors.affiliation: Shantou University Medical College, Shantou 515041, Guangdong, China.
authors.forename: Wen-Xia
authors.initials: WX
authors.lastname: Chen
authors.affiliation: Shantou University Medical College, Shantou 515041, Guangdong, China.
authors.forename: Xin-Bin
authors.initials: XB
authors.lastname: Hong
authors.affiliation: Department of Oncological Research Lab, Cancer Hospital, Shantou University Medical College, Shantou 515041, China.
authors.forename: Chao-Qun
authors.initials: CQ
authors.lastname: Hong
authors.affiliation: Shantou University Medical College, Shantou 515041, Guangdong, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: Shantou University Medical College, Shantou 515041, Guangdong, China.
authors.forename: Lan
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Radiation Oncology, Cancer Hospital, Shantou University Medical College, Shantou 515041, China.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Huang
authors.affiliation: Institute of Oncologic Pathology, Shantou University Medical College, Shantou 515041, China.
authors.forename: Li-Yan
authors.initials: LY
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Cancer Hospital, Shantou University Medical College, Shantou 515041, China. Electronic address: yiwei512@126.com.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, Cancer Hospital, Shantou University Medical College, Shantou 515041, China. Electronic address: pengyuhui666@163.com.
authors.forename: Yu-Hui
authors.initials: YH
authors.lastname: Peng
authors.affiliation: Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515041, China. Electronic address: nmli@stu.edu.cn.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Li
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001323:Autoantibodies / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D002352:Carrier Proteins / Q000097:blood; D016022:Case-Control Studies; D055088:Early Detection of Cancer; D004938:Esophageal Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000276:immunology; D000077277:Esophageal Squamous Cell Carcinoma; D005260:Female; D006801:Humans; D008297:Male; D008840:Microfilament Proteins / Q000097:blood*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D011237:Predictive Value of Tests; D012680:Sensitivity and Specificity
chemical_list: D001323:Autoantibodies; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D008840:Microfilament Proteins; C020324:fascin