title: Trypanocidal activity of the compounds present in Aniba canelilla oil against Trypanosoma evansi and its effects on viability of lymphocytes.
abstract: Aniba canelilla (H.B.K.) Mez, popularly known as "casca-preciosa" (precious bark), is a plant of the Lauraceae family, widely distributed in the Amazon region. Its major constituent is 1-nitro-2-phenylethane, a rare molecule in plants which is responsible for this plant's cinnamon scent. The present study aimed to report the chemical characterization of the oil extracted from Aniba canelilla using gas-chromatography/mass spectrometry and to assess its in vitro trypanocidal activity against Trypanosoma evansi, a prevalent haemoflagellate parasite that affects a broad range of mammal species in Africa, Asia and South America. The oil presented 1-nitro-2-phenylethane (83.68%) and methyleugenol (14.83%) as the two major components. The essential oil as well as both major compounds were shown to exert trypanocidal effect. Methyleugenol was slightly more active than 1-nitro-2-phenylethane. In vitro studies showed that the oil extracted from the stems of A. canelilla may be regarded as a potential natural treatment for trypanosomosis, once proven their in vivo action, may be an interesting alternative in the treatment of infected animals with T. evansi.
authors.affiliation: Laboratório de Desenvolvimento Galênico, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Laboratório de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Regional Integrada do Alto Uruguai e das Missões (URI), Santiago, Brazil.
authors.forename: Janice L
authors.initials: JL
authors.lastname: Giongo
authors.affiliation: Laboratório de Cultura Celular, Ciências da Saúde, Centro Universitário Franciscano (UNIFRA), Santa Maria, Brazil.
authors.forename: Rodrigo A
authors.initials: RA
authors.lastname: Vaucher
authors.affiliation: Departamento de Zootecnia, Universidade do Estado de Santa Catarina (UDESC), Chapecó, Brazil.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Camila B
authors.initials: CB
authors.lastname: Oliveira
authors.affiliation: Laboratório de Desenvolvimento Galênico, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Cristiane B
authors.initials: CB
authors.lastname: de Mattos
authors.affiliation: Laboratório de Cultura Celular, Ciências da Saúde, Centro Universitário Franciscano (UNIFRA), Santa Maria, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Laboratório de Cultura Celular, Ciências da Saúde, Centro Universitário Franciscano (UNIFRA), Santa Maria, Brazil.
authors.forename: Michele R
authors.initials: MR
authors.lastname: Sagrillo
authors.affiliation: Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria (UFSM), Santa Maria, Brazil.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Monteiro
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Manaus, Brazil.
authors.forename: Dayana L
authors.initials: DL
authors.lastname: Custódio
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Manaus, Brazil.
authors.forename: Marcielly
authors.initials: M
authors.lastname: Souza de Matos
authors.affiliation: Instituto Nacional de Pesquisa da Amazônia, Manaus, Brazil.
authors.forename: Paulo T
authors.initials: PT
authors.lastname: Sampaio
authors.affiliation: Laboratório de Desenvolvimento Galênico, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil.
authors.forename: Helder F
authors.initials: HF
authors.lastname: Teixeira
authors.affiliation: Laboratório de Desenvolvimento Galênico, Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, Brazil. Electronic address: leticia.koester@ufrgs.br.
authors.forename: Letícia S
authors.initials: LS
authors.lastname: Koester
authors.affiliation: Departamento de Química, Instituto de Ciências Exatas, Universidade Federal do Amazonas, Manaus, Brazil.
authors.forename: Valdir F
authors.initials: VF
authors.lastname: da Veiga Junior
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D002849:Chromatography, Gas; D019669:Embryophyta / Q000737:chemistry*; D006801:Humans; D008214:Lymphocytes / Q000187:drug effects*; D009822:Oils, Volatile / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D010938:Plant Oils / Q000737:chemistry / Q000494:pharmacology*; D014344:Trypanocidal Agents / Q000737:chemistry / Q000494:pharmacology*; D014345:Trypanosoma / Q000187:drug effects*
chemical_list: D009822:Oils, Volatile; D010936:Plant Extracts; D010938:Plant Oils; D014344:Trypanocidal Agents
keywords: 1-Nitro-2-phenylethane; Aniba canelilla; Antiprotozoal activity; Essential oil composition; Methyleugenol