title: Chitosan and carboxymethyl cellulose based magnetic nanocomposites for application of peroxidase purification.
abstract: Recently, protein purification methods have a very wide area of research. Many of these methods are both expensive and multi-stage methods, that are needed in specific equipment. In this study, biopolymer coated magnetic nanoparticles, carboxymethyl cellulose (CMC) and chitosan (CH) coated Fe3O4(magnetite) nanocomposites, are used in a new purification process. The structure of the synthesized magnetic nanocomposites were characterized by Fourier transform infrared (FTIR) spectrometry, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), thermogravimetric (TGA) analysis and zeta potential for surface charge of magnetic nanocomposites. Molecular weight and purification degree of peroxidase were estimated with SDS-PAGE. Peroxidase enzyme was purified a yield of 82.55% with carboxymethyl cellulose and 76.72% with chitosan using this method.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Bezmialem Vakıf University, Fatih, 34093 Istanbul, Turkey.
authors.forename: Belma
authors.initials: B
authors.lastname: Zengin Kurt
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Bezmialem Vakıf University, Fatih, 34093 Istanbul, Turkey.
authors.forename: Fatih
authors.initials: F
authors.lastname: Uckaya
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Bezmialem Vakıf University, Fatih, 34093 Istanbul, Turkey. Electronic address: zdurmus@bezmialem.edu.tr.
authors.forename: Zehra
authors.initials: Z
authors.lastname: Durmus
mesh_terms: D002266:Carboxymethylcellulose Sodium / Q000737:chemistry*; D005591:Chemical Fractionation / Q000379:methods*; D048271:Chitosan / Q000737:chemistry*; D032084:Citrus sinensis / Q000737:chemistry; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D053761:Nanocomposites / Q000737:chemistry*; D009195:Peroxidase / Q000302:isolation & purification*; D013696:Temperature
chemical_list: D048271:Chitosan; D009195:Peroxidase; D002266:Carboxymethylcellulose Sodium; D052203:Ferrosoferric Oxide
keywords: Carboxymethyl cellulose; Chitosan; Enzyme; Magnetic nanocomposites; Purification