title: The use of heparin chemistry to improve dental osteogenesis associated with implants.
abstract: In this study, we designed a hybrid Ti by heparin modifying the Ti surface followed by Growth/differentiation factor-5 (GDF-5) loading. After that, products were characterized by physicochemical analysis. Quantitative analysis of functionalized groups was also confirmed. The release behavior of GDF-5 grafted samples was confirmed for up to 21days. The surface modification process was found to be successful and to effectively immobilize GDF-5 and provide for its sustained release behavior. As an in vitro test, GDF-5 loaded Ti showed significantly enhanced osteogenic differentiation with increased calcium deposition under nontoxic conditions against periodontal ligament stem cells (PDLSc). Furthermore, an in vivo result showed that GDF-5 loaded Ti had a significant influence on new bone formation in a rabbit model. These results clearly confirmed that our strategy may suggest a useful paradigm by inducing osseo-integration as a means to remodeling and healing of bone defects for restorative procedures in dentistry.
authors.affiliation: Department of Dentistry, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
authors.forename: Sang Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
authors.forename: Min Soo
authors.initials: MS
authors.lastname: Bae
authors.affiliation: Department of Oral and Maxillofacial Surgery, Kyung Hee University Dental Hospital at Gang-dong, 892 Dongnam-ro, Gangdong-gu, Seoul 05278, Republic of Korea.
authors.forename: Deok Won
authors.initials: DW
authors.lastname: Lee
authors.affiliation: Department of Mechanical and Aerospace Engineering, The George Washington University, 801 22nd Street NW, Washington, DC 20052, United States.
authors.forename: Dong Nyoung
authors.initials: DN
authors.lastname: Heo
authors.affiliation: Department of Dentistry, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
authors.forename: Donghyun
authors.initials: D
authors.lastname: Lee
authors.affiliation: Department of Dentistry, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
authors.forename: Min
authors.initials: M
authors.lastname: Heo
authors.affiliation: Department of Prosthodontics, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
authors.forename: Seoung-Jin
authors.initials: SJ
authors.lastname: Hong
authors.affiliation: Department of Prosthodontics, Graduate School, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea.
authors.forename: Joohyoung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials, 156 Gajeongbuk-ro, Yuseong-gu, Daejeon 34103, Republic of Korea.
authors.forename: Wan Doo
authors.initials: WD
authors.lastname: Kim
authors.affiliation: Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials, 156 Gajeongbuk-ro, Yuseong-gu, Daejeon 34103, Republic of Korea. Electronic address: psa@kimm.re.kr.
authors.forename: Su A
authors.initials: SA
authors.lastname: Park
authors.affiliation: Department of Dental Materials, School of Dentistry, Kyung Hee University, 26 Kyungheedae-ro, Dongdaemun-gu, Seoul 02447, Republic of Korea. Electronic address: kwoni@khu.ac.kr.
authors.forename: Il Keun
authors.initials: IK
authors.lastname: Kwon
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D002478:Cells, Cultured; D015921:Dental Implants; D055428:Growth Differentiation Factor 5 / Q000494:pharmacology*; D006493:Heparin / Q000737:chemistry*; D006801:Humans; D010012:Osteogenesis / Q000187:drug effects*; D010513:Periodontal Ligament / Q000166:cytology; D011817:Rabbits; D013234:Stem Cells / Q000187:drug effects; D013499:Surface Properties; D014025:Titanium
chemical_list: D015921:Dental Implants; D055428:Growth Differentiation Factor 5; D006493:Heparin; D014025:Titanium
keywords: Dental bone tissue regeneration; Dental titanium implant; Growth/differentiation factor-5; Heparin; Periodontal ligament stem cells