title: IRAV(FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10.
abstract: Dengue virus (DENV) is a member of the genusFlavivirusand can cause severe febrile illness. Here, we show that FLJ11286, which we refer to as IRAV, is induced by DENV in an interferon-dependent manner, displays antiviral activity against DENV, and localizes to the DENV replication complex. IRAV is an RNA binding protein and localizes to cytoplasmic processing bodies (P bodies) in uninfected cells, where it interacts with the MOV10 RISC complex RNA helicase, suggesting a role for IRAV in the processing of viral RNA. After DENV infection, IRAV, along with MOV10 and Xrn1, localizes to the DENV replication complex and associates with DENV proteins. Depletion of IRAV or MOV10 results in an increase in viral RNA. These data serve to characterize an interferon-stimulated gene with antiviral activity against DENV, as well as to propose a mechanism of activity involving the processing of viral RNA.IMPORTANCEDengue virus, a member of the familyFlaviviridae, can result in a life-threatening illness and has a significant impact on global health. Dengue virus has been shown to be particularly sensitive to the effects of type I interferon; however, little is known about the mechanisms by which interferon-stimulated genes function to inhibit viral replication. A better understanding of the interferon-mediated antiviral response to dengue virus may aid in the development of novel therapeutics. Here, we examine the influence of the interferon-stimulated geneIRAV(FLJ11286) on dengue virus replication. We show that IRAV associates with P bodies in uninfected cells and with the dengue virus replication complex after infection. IRAV also interacts with MOV10, depletion of which is associated with increased viral replication. Our results provide insight into a newly identified antiviral gene, as well as broadening our understanding of the innate immune response to dengue virus infection.
authors.affiliation: Cytokine Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Corey A
authors.initials: CA
authors.lastname: Balinsky
authors.affiliation: Cytokine Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Hana
authors.initials: H
authors.lastname: Schmeisser
authors.affiliation: Cytokine Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Alexandra I
authors.initials: AI
authors.lastname: Wells
authors.affiliation: Biological Imaging Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Sundar
authors.initials: S
authors.lastname: Ganesan
authors.affiliation: Structural Immunobiology Unit, Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Tengchuan
authors.initials: T
authors.lastname: Jin
authors.affiliation: Structural Biology Section, Research Technologies Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA.
authors.forename: Kavita
authors.initials: K
authors.lastname: Singh
authors.affiliation: Cytokine Biology Section, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA kzoon@niaid.nih.gov.
authors.forename: Kathryn C
authors.initials: KC
authors.lastname: Zoon
mesh_terms: D000072283:A549 Cells; D000330:Aedes; D000818:Animals; D002522:Chlorocebus aethiops; D003716:Dengue Virus / Q000502:physiology*; D015870:Gene Expression; D054884:Host-Pathogen Interactions; D006801:Humans; D007113:Immunity, Innate; D021381:Protein Transport; D020365:RNA Helicases / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000502:physiology*; D015533:Transcriptional Activation / Q000276:immunology*; D015854:Up-Regulation; D014709:Vero Cells; D014779:Virus Replication
chemical_list: D016601:RNA-Binding Proteins; C000619495:SHFL protein, human; C530476:Mov10 protein, human; D020365:RNA Helicases
keywords: C19orf66; FLJ11286; IRAV; MOV10; P body; UPF0515; dengue; flavivirus; interferon