title: Differential effects of type 2 diabetes on brain glycometabolism in rats: focus on glycogen and monocarboxylate transporter 2.
abstract: Astrocyte-neuron lactate shuttle (ANLS) is a pathway that supplies glycogen-derived lactate to active neurons via monocarboxylate transporter 2 (MCT2), and is important for maintaining brain functions. Our study revealed alterations of ANLS with hippocampal hyper-glycogen levels and downregulated MCT2 protein levels underlying hippocampal dysfunctions as a complication in type 2 diabetic (T2DM) animals. Since T2DM rats exhibit brain dysfunctions involving several brain regions, we examined whether there might also be T2DM effects on ANLS's disturbances in other brain loci. OLETF rats exhibited significantly higher glycogen levels in the hippocampus, hypothalamus, and cerebral cortex than did LETO rats. MCT2 protein levels in OLETF rats decreased significantly in the hippocampus and hypothalamus compared to their controls, but a significant correlation with glycogen levels was only observed in the hippocampus. This suggests that the hippocampus may be more vulnerable to T2DM compared to other brain regions in the context of ANLS disruption.
authors.affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan.
authors.forename: Takeru
authors.initials: T
authors.lastname: Shima
authors.affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan.|Department of Sports Neuroscience, Advanced Research Initiative for Human High Performance (ARIHHP), University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan.
authors.forename: Subrina
authors.initials: S
authors.lastname: Jesmin
authors.affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan.|Department of Sports Neuroscience, Advanced Research Initiative for Human High Performance (ARIHHP), University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Matsui
authors.affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Soya
authors.affiliation: Laboratory of Exercise Biochemistry and Neuroendocrinology, Faculty of Health and Sport Sciences, University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan. hsoya@taiiku.tsukuba.ac.jp.|Department of Sports Neuroscience, Advanced Research Initiative for Human High Performance (ARIHHP), University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, Ibaraki, 305-8574, Japan. hsoya@taiiku.tsukuba.ac.jp.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Soya
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000378:metabolism; D002540:Cerebral Cortex / Q000378:metabolism*; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism*; D006624:Hippocampus / Q000378:metabolism*; D008297:Male; D027501:Monocarboxylic Acid Transporters / Q000378:metabolism*; D009474:Neurons / Q000378:metabolism; D051381:Rats; D020334:Rats, Inbred OLETF
chemical_list: D027501:Monocarboxylic Acid Transporters; D006003:Glycogen
keywords: Astrocyte-neuron lactate shuttle; Brain glycogen; Hippocampus; Monocarboxylate transporter; Type 2 diabetes mellitus