title: The E-Subgroup Pentatricopeptide Repeat Protein Family inArabidopsis thalianaand Confirmation of the ResponsivenessPPR96to Abiotic Stresses.
abstract: Pentatricopeptide repeat (PPR) proteins are extensive in all eukaryotes. Their functions remain as yet largely unknown. Mining potential stress responsive PPRs, and checking whether known PPR editing factors are affected in the stress treatments. It is beneficial to elucidate the regulation mechanism of PPRs involved in biotic and abiotic stress. Here, we explored the characteristics and origin of the 105 E subgroup PPRs inArabidopsis thaliana. Phylogenetic analysis categorized the E subgroup PPRs into five discrete groups (Cluster I to V), and they may have a common origin in bothA. thalianaand rice. Anin silicoexpression analysis of the 105 E subgroup PPRs inA. thalianawas performed using available microarray data. Thirty-four PPRs were differentially expressed duringA. thalianaseed imbibition, seed development stage(s), and flowers development processes. To explore potential stress responsive PPRs, differential expression of 92 PPRs was observed inA. thalianaseedlings subjected to different abiotic stresses. qPCR data of E subgroup PPRs under stress conditions revealed that the expression of 5 PPRs was responsive to abiotic stresses. In addition,PPR96is involved in plant responses to salt, abscisic acid (ABA), and oxidative stress. The T-DNA insertion mutation inactivatingPPR96expression results in plant insensitivity to salt, ABA, and oxidative stress. The PPR96 protein is localized in the mitochondria, and altered transcription levels of several stress-responsive genes under abiotic stress treatments. Our results suggest thatPPR96may important function in a role connecting the regulation of oxidative respiration and environmental responses inA. thaliana.
authors.affiliation: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal UniversityHarbin, China; Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementBeijing, China.
authors.forename: Jia-Ming
authors.initials: JM
authors.lastname: Liu
authors.affiliation: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal UniversityHarbin, China; Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic ImprovementBeijing, China.
authors.forename: Juan-Ying
authors.initials: JY
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Beijing, China.
authors.forename: Pan-Pan
authors.initials: PP
authors.lastname: Lu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Beijing, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University Harbin, China.
authors.forename: Chang-Hong
authors.initials: CH
authors.lastname: Guo
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Beijing, China.
authors.forename: Zhao-Shi
authors.initials: ZS
authors.lastname: Xu
authors.affiliation: Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Institute of Crop Science, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Beijing, China.
authors.forename: You-Zhi
authors.initials: YZ
authors.lastname: Ma
keywords: Arabidopsis thaliana; abiotic stresses; flower development processes; microarray analysis; mitochondria; pentatricopeptide repeat (PPR) proteins; seed development stage(s); seed imbibition