title: Iron restriction increases myoglobin gene and protein expression in Soleus muscle of rats.
abstract: Iron is an important trace element for proper cell functioning. It is present in cytochromes, hemoglobin and myoglobin (Mb), where it binds to oxygen. It is also an electron acceptor in the respiratory chain. Mb is an 18 kDa heme-protein, highly expressed in skeletal muscle and heart. The expression of several genes involved in the metabolism of iron is post-transcriptionally regulated by this element. Iron was shown to interfere with the polyadenylation step, modifying their poly (A) tail length and, as a consequence, their stability and translation rate. The aim of this study was to investigate whether iron supplementation or long and short-term restriction affects Mb gene and protein expression, as well as Mb mRNA poly(A) tail length, in cardiac and skeletal muscles of rats. Long-term iron restriction caused an increase in Mb gene and protein expression in Soleus muscle. No changes were observed in extensor digitorum longus muscle and heart. Short-term iron supplementation after iron deprivation did not alter Mb gene expression and mRNA poly(A) tail length in all tissues studied. These results indicate that Mb gene and protein expression is upregulated in response to iron deprivation, an effect that is tissue-specific and seems to occur at transcriptional level.
authors.affiliation: Instituto de Ciências Biomédicas/ICB, Universidade de São Paulo, Departamento de Fisiologia e Biofísica, Laboratório 129, Avenida Professor Lineu Prestes, 1524, Butantan, 05508-900 São Paulo, SP, Brazil.|Universidade Federal de São Paulo, Escola Paulista de Medicina, Departamento de Medicina, Laboratório Endocrinologia Molecular e Translacional, Divisão de Endocrinologia, Rua Pedro de Toledo, 669, 11º andar, Vila Clementino, 04039-032 São Paulo, SP, Brazil.
authors.forename: Janaina S DE
authors.initials: JS
authors.lastname: Souza
authors.affiliation: Instituto de Ciências Biomédicas/ICB, Universidade de São Paulo, Departamento de Fisiologia e Biofísica, Laboratório 129, Avenida Professor Lineu Prestes, 1524, Butantan, 05508-900 São Paulo, SP, Brazil.
authors.forename: Erika L
authors.initials: EL
authors.lastname: Brunetto
authors.affiliation: Instituto de Ciências Biomédicas/ICB, Universidade de São Paulo, Departamento de Fisiologia e Biofísica, Laboratório 129, Avenida Professor Lineu Prestes, 1524, Butantan, 05508-900 São Paulo, SP, Brazil.
authors.forename: Maria Tereza
authors.initials: MT
authors.lastname: Nunes
mesh_terms: D000818:Animals; D015870:Gene Expression / Q000187:drug effects*; D007501:Iron / Q000494:pharmacology*; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009211:Myoglobin / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D051381:Rats
chemical_list: D009211:Myoglobin; D012333:RNA, Messenger; D007501:Iron