title: Evaluation of In Vitro Antimalarial Activity of Different Extracts ofEremostachys azerbaijanicaRech.f.
abstract: Six extracts with different polarity from aerial parts and rhizomes ofEremostachys azerbaijanicaRech.f., were screened for their antimalarial properties bycell free𝛽-hematin formation assay. Dichloromethane (DCM) extracts of both parts of plant showed significant antimalarial activities with IC50values of 0.949 ± 0.061 mg/mL in aerial parts and 0.382 ± 0.011 mg/mL in rhizomes. Bioactivity-guided fractionation of the most potent part (DCM extract of rhizomes) by vacuum liquid chromatography (VLC) afforded seven fractions. Two fractions [100% Ethyl acetate (EtOAC) and 100% Methatol (MeOH)] showed considerable antimalarial activity with IC50values of 0.335 ± 0.033 mg/mL and 0.403 ± 0.037 mg/mL, respectively. According to GC-MS analysis, the sesquiterpene, steroid and coumarin derivatives are the main constituents of the most potent fractions; therefore, it seems that the anti malarial activity of these fractions may be related to the presence of these types of compounds.
authors.affiliation: Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Solmaz
authors.initials: S
authors.lastname: Asnaashari
authors.affiliation: Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Heshmati Afshar
authors.affiliation: Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Sedigheh
authors.initials: S
authors.lastname: Bamdad Moghadam
authors.affiliation: Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran. ; Department of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Delazar
keywords: Antimalaria; Cell free assay; Eremostachys azerbaijanica; GC-MS