title: Chemical structure, antiproliferative and antioxidant activities of a cell wall α-d-mannan from yeast Kluyveromyces marxianus.
abstract: Cell wall polysaccharides from filamentous fungi and yeasts have been reported as antioxidant and antiproliferative polymers. Thus, we evaluated these activities from cell wall polysaccharides from Kluyveromyces marxianus CCT7735. By using a centrifugal filter, a 203kDa α-d-mannan (KMM-5) was obtained. KMM-5 exhibited no effect on HeLa cells and a weak antiproliferative activity against Hep-G2 cells. In addition, at higher concentrations, it presented a cytotoxicity to the normal cell line, 3T3. However, KMM-5 showed copper- and iron-chelating abilities, the latter of which presented improved activity. By using 2D-NMR COSY, HSQC edited and HMBC experiments, a structure arrangement was proposed. The main chain was formed by 6)-α-d-Manp-(1→6) units substituted at the 2-O-position by non-reducing terminals α-d-Manp-(1→2) and by a branched tetrasaccharide. The latter was formed by an internal 2)-α-d-Manp-(1→2) unit with linked to it a 2,3)-α-d-Manp-(1→2) unit substituted at the 2-O-position by a non-reducing terminal α-d-Manp-(1→2), and at the 3-O-position by a non-reducing terminal α-d-Manp-(1→3). In conclusion, we considered K. marxianus CCT7735 a source of natural and renewable polysaccharides with pharmacological properties.
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36.570-900, Brazil. Electronic address: edergalinari@yahoo.com.br.
authors.forename: Éder
authors.initials: É
authors.lastname: Galinari
authors.affiliation: Department of Biochemistry, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. Electronic address: popoh_diego@hotmail.com.
authors.forename: Diego Araújo
authors.initials: DA
authors.lastname: Sabry
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal do Paraná, Curitiba, Paraná 81.531-980, Brazil. Electronic address: sassaki@ufpr.br.
authors.forename: Guilherme Lanzi
authors.initials: GL
authors.lastname: Sassaki
authors.affiliation: Department of Chemistry Engineering, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. Electronic address: gomacedo@eq.ufrn.br.
authors.forename: Gorete Ribeiro
authors.initials: GR
authors.lastname: Macedo
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36.570-900, Brazil. Electronic address: flopespassos@gmail.com.
authors.forename: Flávia Maria Lopes
authors.initials: FML
authors.lastname: Passos
authors.affiliation: Department of Microbiology, Universidade Federal de Viçosa, Viçosa, Minas Gerais 36.570-900, Brazil. Electronic address: hcmantovani@gmail.com.
authors.forename: Hilário Cuquetto
authors.initials: HC
authors.lastname: Mantovani
authors.affiliation: Department of Biochemistry, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte 59.078-970, Brazil. Electronic address: hugo@cb.ufrn.br.
authors.forename: Hugo Alexandre Oliveira
authors.initials: HAO
authors.lastname: Rocha
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D049109:Cell Proliferation / Q000187:drug effects; D002473:Cell Wall / Q000737:chemistry*; D006367:HeLa Cells; D017325:Hepatitis B Vaccines; D006801:Humans; D007716:Kluyveromyces / Q000737:chemistry*; D008351:Mannans / Q000737:chemistry*; D051379:Mice
chemical_list: D000975:Antioxidants; C089444:Hepa Gene 3 vaccine; D017325:Hepatitis B Vaccines; D008351:Mannans
keywords: Copper chelator; Iron chelator; NMR; Polysaccharide; Tumor cell