title: Hyaluronic acid doped-poly(3,4-ethylenedioxythiophene)/chitosan/gelatin (PEDOT-HA/Cs/Gel) porous conductive scaffold for nerve regeneration.
abstract: Conducting polymer, as a "smart" biomaterial, has been increasingly used to construct tissue engineered scaffold for nerve tissue regeneration. In this study, a novel porous conductive scaffold was prepared by incorporating conductive hyaluronic acid (HA) doped-poly(3,4-ethylenedioxythiophene) (PEDOT-HA) nanoparticles into a chitosan/gelatin (Cs/Gel) matrix. The physicochemical characteristics of Cs/Gel scaffold with 0-10wt% PEDOT-HA were analyzed and the results indicated that the incorporation of PEDOT-HA into scaffold increased the electrical and mechanical properties while decreasing the porosity and water absorption. Moreover, in vitro biodegradation of scaffold displayed a declining trend with the PEDOT-HA content increased. About the biocompatibility of conductive scaffold, neuron-like rat phaeochromocytoma (PC12) cells were cultured in scaffold to evaluate cell adhesion and growth. 8% PEDOT-HA/Cs/Gel scaffold had a higher cell adhesive efficiency and cell viability than the other conductive scaffolds. Furthermore, cells in the scaffold with 8wt% PEDOT-HA expressed higher synapse growth gene of GAP43 and SYP compared with Cs/Gel control group. These results suggest that 8%PEDOT-HA/Cs/Gel scaffold is an attractive cell culture conductive substrate which could support cell adhesion, survival, proliferation, and synapse growth for the application in nerve tissue regeneration.
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, P.R. China.
authors.forename: Shuping
authors.initials: S
authors.lastname: Wang
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, P.R. China. Electronic address: guanshui@dlut.edu.cn.
authors.forename: Shui
authors.initials: S
authors.lastname: Guan
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, P.R. China.
authors.forename: Zhibo
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, P.R. China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Li
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, P.R. China.
authors.forename: Tianqing
authors.initials: T
authors.lastname: Liu
authors.affiliation: Dalian R&D Center for Stem Cell and Tissue Engineering, Dalian University of Technology, Dalian 116024, P.R. China.
authors.forename: Xuehu
authors.initials: X
authors.lastname: Ma
mesh_terms: D000818:Animals; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects*; D005782:Gels; D006820:Hyaluronic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D009416:Nerve Regeneration / Q000187:drug effects*; D016716:PC12 Cells; D011108:Polymers / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D051381:Rats; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D005782:Gels; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D006820:Hyaluronic Acid
keywords: Chitosan and gelatin; Conducting polymer; Nerve regeneration; PC12 cells; Porous scaffold