title: Simultaneous detection of four nitrofuran metabolites in honey by using a visualized microarray screen assay.
abstract: A visualized microarray sensing technique has been developed and applied to the screening of honey samples for residues of banned nitrofuran antibiotics. Using a multiplexed approach, metabolites of four main nitrofuran antibiotics can be detected simultaneously. Individual antigens were spotted onto 96-well plates. An indirective competitive assay format, with visualized signal response, was employed. An extraction method, based on derivatization with 2-nitrobenzaldehyde and partition into ethyl acetate, was used for screening. The limits of detection were 0.10, 0.04, 0.04, and 0.10ngg-1for 3-amino-5-morpholino-2-oxazolidone (AMOZ), 3-amino-2-oxazolidinone (AOZ), semicarbazide (SEM), and 1-aminohydantoin (AHD), respectively. The recovery rate ranged from 78% to 93% for the four targets. In addition, this method was easy to operate with low detection cost and fast speed. This microarray method possesses the potential to be a fit-for-purpose screening technique in the arena of food safety monitoring.
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: lizhonghui0106@gmail.com.
authors.forename: Zhonghui
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China; School of Chemistry and Biological Science, Nanjing University Jingling College, Nanjing 210089, China. Electronic address: lizhoumin@126.com.
authors.forename: Zhoumin
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China. Electronic address: xudanke@nju.edu.cn.
authors.forename: Danke
authors.initials: D
authors.lastname: Xu
mesh_terms: D000085:Acetates / Q000032:analysis; D000900:Anti-Bacterial Agents / Q000737:chemistry; D001547:Benzaldehydes / Q000032:analysis; D005504:Food Analysis; D063427:Food Quality; D059022:Food Safety; D006722:Honey / Q000032:analysis*; D006827:Hydantoins / Q000032:analysis; D057230:Limit of Detection; D046228:Microarray Analysis / Q000379:methods*; D009581:Nitrofurans / Q000737:chemistry*; D023303:Oxazolidinones / Q000032:analysis; D015203:Reproducibility of Results; D012663:Semicarbazides / Q000032:analysis
chemical_list: C504350:1-aminohydantoin; D000085:Acetates; D000900:Anti-Bacterial Agents; D001547:Benzaldehydes; D006827:Hydantoins; D009581:Nitrofurans; D023303:Oxazolidinones; D012663:Semicarbazides; C010059:carbamylhydrazine; C043850:2-nitrobenzaldehyde; C007650:ethyl acetate; C000209:3-amino-2-oxazolidinone
keywords: 2-NP-AHD (PubChem CID: 9690702); 2-NP-AMOZ (PubChem CID: 53393538); 2-NP-AOZ (PubChem CID: 209292); 2-NP-SEM (PubChem CID: 6870110); AHD (PubChem CID: 72823); AMOZ (PubChem CID: 3016406); AOZ (PubChem CID: 65725); Chloroauric acid (PubChem CID: 28133); Nitrofuran metabolites; SEM (PubChem CID: 5196); Silver nanoparticles; Silver nitrate (PubChem CID: 24470); Visualized microarray assay