title: Redox properties of extracellular polymeric substances (EPS) from electroactive bacteria.
abstract: Although the capacity for electroactive bacteria to convert environmental metallic minerals and organic pollutants is well known, the role of the redox properties of microbial extracellular polymeric substances (EPS) in this process is poorly understood. In this work, the redox properties of EPS from two widely present electroactive bacterial strains (Shewanella oneidensis and Pseudomonas putida) were explored. Electrochemical analysis demonstrates that the EPS extracted from the two strains exhibited redox properties. Spectroelectrochemical and protein electrophoresis analyses indicate that the extracted EPS from S. oneidensis and P. putida contained heme-binding proteins, which were identified as the possible redox components in the EPS. The results of heme-mediated behavior of EPS may provide an insight into the important roles of EPS in electroactive bacteria to maximize their redox capability for biogeochemical cycling, environmental bioremediation and wastewater treatment.
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.
authors.forename: Shan-Wei
authors.initials: SW
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.
authors.forename: Guo-Ping
authors.initials: GP
authors.lastname: Sheng
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Cheng
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Department of Chemistry, University of Science and Technology of China, Hefei, 230026, China.
authors.forename: Han-Qing
authors.initials: HQ
authors.lastname: Yu
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001673:Biodegradation, Environmental; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism; D055664:Electrochemical Techniques; D005110:Extracellular Space / Q000378:metabolism*; D000081062:Heme-Binding Proteins; D006420:Hemeproteins / Q000737:chemistry / Q000378:metabolism; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry* / Q000378:metabolism; D016958:Pseudomonas putida / Q000737:chemistry / Q000378:metabolism*; D020592:Shewanella / Q000737:chemistry / Q000378:metabolism*
chemical_list: D001426:Bacterial Proteins; D002352:Carrier Proteins; D000081062:Heme-Binding Proteins; D006420:Hemeproteins; D011108:Polymers