title: Kinetics of the association of dengue virus capsid protein with the granular component of nucleolus.
abstract: Dengue virus (DENV) replicates in the cytoplasm but translocation of the capsid protein (C) to the nucleoli of infected cells has been shown to facilitate virus multiplication for DENV-2. This study demonstrates that the nucleolar localization of C occurs with all four serotypes of DENV. The interaction of C with the nucleolus was found to be dynamic with a mobile fraction of 66% by FRAP. That the C shuttled between the nucleus and cytoplasm was suggested by FLIP and translation inhibition experiments. Colocalization with B23 indicated that DENV C targeted the granular component (GC) of the nucleolus. Presence of DENV C in the nucleolus affected the recovery kinetics of B23 in infected and transfected cells. Sub-nucleolar localization of DENV C of all serotypes to the GC, its mobility in and out of the nucleolus and its affect on the dynamics of B23 is being shown for the first time.
authors.affiliation: Dengue Group, National Institute of Virology, 20-A Ambedkar Road, Pune 411001, Maharashtra, India.
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Tiwary
authors.affiliation: Dengue Group, National Institute of Virology, 20-A Ambedkar Road, Pune 411001, Maharashtra, India. Electronic address: cecilia.dayaraj@gmail.com.
authors.forename: D
authors.initials: D
authors.lastname: Cecilia
mesh_terms: D036022:Capsid Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D002466:Cell Nucleolus / Q000737:chemistry / Q000378:metabolism / Q000821:virology*; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D003715:Dengue / Q000378:metabolism / Q000821:virology*; D003716:Dengue Virus / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007700:Kinetics; D009687:Nuclear Proteins / Q000378:metabolism; D000090243:Nucleophosmin; D021381:Protein Transport
chemical_list: D036022:Capsid Proteins; D009687:Nuclear Proteins; D000090243:Nucleophosmin
keywords: B23; Capsid; Dengue; FLIP; FRAP; Granular component; Nucleolus; Virus