title: cGAL, a temperature-robust GAL4-UAS system for Caenorhabditis elegans.
abstract: The GAL4-UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4-UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit.
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.|Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
authors.forename: Han
authors.identifier: 0000-0002-1933-5762
authors.initials: H
authors.lastname: Wang
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.|Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Liu
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.|Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
authors.forename: Shahla
authors.initials: S
authors.lastname: Gharib
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.|Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
authors.forename: Cynthia M
authors.initials: CM
authors.lastname: Chai
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.|Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
authors.forename: Erich M
authors.identifier: 0000-0003-3151-4381
authors.initials: EM
authors.lastname: Schwarz
authors.affiliation: Department of Life Sciences, New York Institute of Technology, Old Westbury, New York, USA.
authors.forename: Navin
authors.identifier: 0000-0003-2323-6190
authors.initials: N
authors.lastname: Pokala
authors.affiliation: Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.|Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California, USA.
authors.forename: Paul W
authors.identifier: 0000-0002-7699-0173
authors.initials: PW
authors.lastname: Sternberg
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017173:Caenorhabditis elegans / Q000235:genetics*; D004268:DNA-Binding Proteins / Q000235:genetics*; D003672:Defecation / Q000235:genetics; D005786:Gene Expression Regulation; D005818:Genetic Engineering / Q000379:methods*; D018259:Herpesvirus 1, Human / Q000235:genetics; D008856:Microscopy, Fluorescence; D062308:Optogenetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*; D013696:Temperature; D014157:Transcription Factors / Q000235:genetics*
chemical_list: D004268:DNA-Binding Proteins; C052130:GAL4 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors