title: Effect of tributyltin on antioxidant ability and immune responses of zebrafish (Danio rerio).
abstract: Tributyltin (TBT) is a toxic compound released into aquatic ecosystems through antifouling paints. This study was designed to examine the effects of TBT on antioxidant ability and immune responses of zebrafish (Danio rerio). Three hundred sixty healthy zebrafish were randomly grouped into four groups and exposed to different doses of TBT (0, 1, 10 and 100ngL-1). At the end of 8 weeks, the fish were sampled, and antioxidant capability, immune parameters and immune-related genes were assessed. The results showed that with an increase in TBT dose, the concentration of malonaldehyde in the liver was significantly increased (p<0.05), whereas the activities of total superoxide dismutase, catalase and glutathione peroxidase were significantly decreased (p<0.05) compared to the control. The activity and expression of lysozyme and the content of immunoglobulin M were significantly decreased compared to those of the fish exposed to 0ngL-1TBT (p<0.05). However, the expression of the HSP70, HSP90, tumor necrosis factor-α(TNF-α), interleukins (IL-1β, IL-6), and nuclear factor-kappa B p65 (NF-κ B p65) genes were all enhanced with an increase in TBT dose. The results indicated that TBT induced oxidative stress and had immunotoxic effects on zebrafish.
authors.affiliation: College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China. Electronic address: zhangchunnuan12@163.com.
authors.forename: Chun-Nuan
authors.initials: CN
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.
authors.forename: Ji-Liang
authors.initials: JL
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.
authors.forename: Hong-Tao
authors.initials: HT
authors.lastname: Ren
authors.affiliation: College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.
authors.forename: Bian-Hua
authors.initials: BH
authors.lastname: Zhou
authors.affiliation: College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.
authors.forename: Qiu-Jue
authors.initials: QJ
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Henan University of Scientific and Technology, Luoyang 471003, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Sun
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002374:Catalase / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism; D004202:Disinfectants / Q000633:toxicity*; D005979:Glutathione Peroxidase / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D007109:Immunity / Q000187:drug effects*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008315:Malondialdehyde / Q000378:metabolism; D009113:Muramidase / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D011897:Random Allocation; D013482:Superoxide Dismutase / Q000378:metabolism; D014220:Trialkyltin Compounds / Q000378:metabolism / Q000633:toxicity*; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*; D015027:Zebrafish / Q000276:immunology / Q000378:metabolism
chemical_list: D000975:Antioxidants; D016207:Cytokines; D004202:Disinfectants; D006360:Heat-Shock Proteins; D014220:Trialkyltin Compounds; D014409:Tumor Necrosis Factor-alpha; D014874:Water Pollutants, Chemical; C011559:tributyltin; D008315:Malondialdehyde; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D009113:Muramidase
keywords: Antioxidant capability; Danio rerio; Immune response; Tributyltin