title: Validation of the Microreader™ 23sp ID system: A new STR 23-plex system for forensic application.
abstract: Microreader™ 23sp ID system is a new 23-plex STR genotyping system that amplified 21 non-CODIS STR loci (D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D7S3048, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D12S391, D2S1338, D17S1290 and D5S2500), one CODIS STR locus (D16S539) and the amelogenin locus in one reaction. Microreader™ 23sp ID system was validated according to the guidelines of "Validation Guidelines for DNA Analysis Methods (2012)" described by the Scientific Working Group on DNA Analysis Methods (SWGDAM), including PCR-based studies, sensitivity study, precision and accuracy evaluation, stutter percentage and peak height ratio, inhibitors, species specificity and DNA mixture studies. Our results suggested that Microreader™ 23sp ID system is a useful tool for identification and parentage testing.
authors.affiliation: Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China.
authors.forename: Jienan
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Luo
authors.affiliation: Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Song
authors.affiliation: Department of Pathology and Pathophysiology, Chengdu Medical College, Chengdu 610500, Sichuan, China.
authors.forename: Lushun
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China.
authors.forename: Chuncao
authors.initials: C
authors.lastname: Deng
authors.affiliation: Suzhou Microread Genetics, Suzhou 215163, Jiangsu, China.
authors.forename: Zailiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China.
authors.forename: Tianzhen
authors.initials: T
authors.lastname: Gao
authors.affiliation: Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Liao
authors.affiliation: Department of Forensic Genetics, West China School of Pre-clinical and Forensic Medicine, Sichuan University (West China University of Medical Sciences), Chengdu 610041, Sichuan, China. Electronic address: profhou@yahoo.com.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Hou
mesh_terms: D053523:Amelogenin / Q000235:genetics; D000818:Animals; D044466:Asian People / Q000235:genetics; D002681:China; D016172:DNA Fingerprinting; D005006:Ethnicity / Q000235:genetics; D005838:Genotype; D006801:Humans; D018895:Microsatellite Repeats; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods*; D013045:Species Specificity
chemical_list: D053523:Amelogenin
keywords: Forensic science; Microreader™ 23sp ID system; Short tandem repeat (STR); Validation