title: In vitro and in vivo assessment of nanotoxicity of CdS quantum dot/aminopolysaccharide bionanoconjugates.
abstract: The nanotoxicity of Cd-containing quantum dots (QDs) for biomedical applications is very controversial and not completely understood. In this study, we evaluated the cytotoxicity of surface-biofunctionalized CdS QDs with chitosan directly synthesized via aqueous route at room temperature. These core-shell CdS-chitosan nanoconjugates showed different degrees of cytotoxic responses using MTT cell proliferation assay toward three human cell cultures, human osteosarcoma cell line (SAOS), non-Hodgkin's B cell lymphoma (Toledo), and human embryonic kidney cell line (HEK293T), under three exposure times (1, 3, and 5days) and three colloidal concentrations (10nM, 50nM, and 100nM). The results clearly demonstrated that the CdS QDs, regardless to the fact that they were coated with a biocompatible aminopolysaccharide shell, induced a severe dose- and time-dependent inhibition of cell viability. In addition, the HEK293T and SAOS cell lines showed much more sensitive response compared to Toledo, which indicated that the cytotoxicity was also cell-type dependent. The exceptional resistance of Toledo cells to toxic effects of CdS nanoconjugates even at severe test conditions was assigned to specific role of B-lineage cells of the immune defense system. Remarkably, no conclusive evidence of toxicity of CdS nanoconjugates was observed in vivo using intravenous injections of CdS nanoconjugates in BALB/c mouse animal models for 30days, but localized fluorescence was detected in ex-vivo liver tissue samples. Therefore, these results prove that there is no guarantee of "risk-free" use of CdS nanoconjugates for in vivo applications, even when functionalized with biopolymer ligands, as they can pose an excessive threat due to unpredicted and uncorrelated responses under in vitro and in vivo biological assays with highly toxic cadmium ions.
authors.affiliation: Center of Nanoscience, Nanotechnology, and Innovation-CeNano(2)I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, UFMG, Brazil; Department of Physiology and Biophysics, ICB, UFMG, Brazil.
authors.forename: S M
authors.initials: SM
authors.lastname: de Carvalho
authors.affiliation: Center of Nanoscience, Nanotechnology, and Innovation-CeNano(2)I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, UFMG, Brazil.
authors.forename: A A P
authors.initials: AAP
authors.lastname: Mansur
authors.affiliation: Center of Nanoscience, Nanotechnology, and Innovation-CeNano(2)I, Department of Metallurgical and Materials Engineering, Federal University of Minas Gerais, UFMG, Brazil. Electronic address: hmansur@demet.ufmg.br.
authors.forename: H S
authors.initials: HS
authors.lastname: Mansur
authors.affiliation: Department of Preventive Veterinary Medicine, Veterinary School, UFMG, Brazil.
authors.forename: M I M C
authors.initials: MIMC
authors.lastname: Guedes
authors.affiliation: Department of Preventive Veterinary Medicine, Veterinary School, UFMG, Brazil.
authors.forename: Z I P
authors.initials: ZIP
authors.lastname: Lobato
authors.affiliation: Department of Physiology and Biophysics, ICB, UFMG, Brazil.
authors.forename: M F
authors.initials: MF
authors.lastname: Leite
mesh_terms: D000818:Animals; D019187:Cadmium Compounds / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D045744:Cell Line, Tumor; D048271:Chitosan / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology; D057809:HEK293 Cells; D006801:Humans; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D045663:Quantum Dots / Q000737:chemistry*; D013440:Sulfides / Q000009:adverse effects / Q000737:chemistry / Q000494:pharmacology
chemical_list: D019187:Cadmium Compounds; D013440:Sulfides; C034939:cadmium sulfide; D048271:Chitosan
keywords: Bioconjugates; Biomarkers; Fluorescent biomarkers; Functionalized nanoparticles; Nanoconjugates; Nanotoxicity; Semiconductor nanoparticles; Toxicity