title: The minimum information required for a glycomics experiment (MIRAGE) project: improving the standards for reporting glycan microarray-based data.
abstract: MIRAGE (Minimum Information Required for A Glycomics Experiment) is an initiative that was created by experts in the fields of glycobiology, glycoanalytics and glycoinformatics to produce guidelines for reporting results from the diverse types of experiments and analyses used in structural and functional studies of glycans in the scientific literature. As a sequel to the guidelines for sample preparation (Struwe et al. 2016, Glycobiology, 26:907-910) and mass spectrometry  data (Kolarich et al. 2013, Mol. Cell Proteomics, 12:991-995), here we present the first version of guidelines intended to improve the standards for reporting data from glycan microarray analyses. For each of eight areas in the workflow of a glycan microarray experiment, we provide guidelines for the minimal information that should be provided in reporting results. We hope that the MIRAGE glycan microarray guidelines proposed here will gain broad acceptance by the community, and will facilitate interpretation and reproducibility of the glycan microarray results with implications in comparison of data from different laboratories and eventual deposition of glycan microarray data in international databases.
authors.affiliation: Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: Ryan
authors.initials: R
authors.lastname: McBride
authors.affiliation: Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Stoll
authors.affiliation: Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.|Department of Chemistry, UCIBIO@REQUIMTE,  Faculty of Science and Technology, NOVA University of Lisbon, Caparica 2829-516, Portugal.
authors.forename: Angelina S
authors.initials: AS
authors.lastname: Palma
authors.affiliation: Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Lisete
authors.initials: L
authors.lastname: Silva
authors.affiliation: Department of Surgery, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Agravat
authors.affiliation: Department of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, 1-236 Tangimachi, Hachioji, Tokyo 192-8577, Japan.
authors.forename: Kiyoko F
authors.initials: KF
authors.lastname: Aoki-Kinoshita
authors.affiliation: Biomolecular Frontiers Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Matthew P
authors.initials: MP
authors.lastname: Campbell
authors.affiliation: Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University, School of Medicine, 670 Albany Street, Suite 504, Boston, MA 02118, USA.
authors.forename: Catherine E
authors.initials: CE
authors.lastname: Costello
authors.affiliation: Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Anne
authors.initials: A
authors.lastname: Dell
authors.affiliation: Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, UK.
authors.forename: Stuart M
authors.initials: SM
authors.lastname: Haslam
authors.affiliation: Department of Medical Biochemistry and Cell Biology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, PO Box 440, 405 30 Gothenburg, Sweden.
authors.forename: Niclas G
authors.initials: NG
authors.lastname: Karlsson
authors.affiliation: Institute of Biological Chemistry, Academia Sinica, 128, Academia Road Sec. 2, Nankang, Taipei 115, Taiwan.
authors.forename: Kay-Hooi
authors.initials: KH
authors.lastname: Khoo
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces, Potsdam 14424, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Kolarich
authors.affiliation: Department of Chemistry, Indiana University, 800 E. Kirkwood Avenue, Bloomington, IN 47405, USA.
authors.forename: Milos V
authors.initials: MV
authors.lastname: Novotny
authors.affiliation: Biomolecular Frontiers Research Centre, Macquarie University, Sydney, NSW 2109, Australia.
authors.forename: Nicolle H
authors.initials: NH
authors.lastname: Packer
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Rene
authors.initials: R
authors.lastname: Ranzinger
authors.affiliation: Max Planck Institute for Dynamics of Complex Technical Systems, Bioprocess Engineering, 39106 Magdeburg, Germany.
authors.forename: Erdmann
authors.initials: E
authors.lastname: Rapp
authors.affiliation: NIBRT GlycoScience Group, NIBRT-National Institute for Bioprocessing Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co., Dublin, Ireland.
authors.forename: Pauline M
authors.initials: PM
authors.lastname: Rudd
authors.affiliation: Department of Biochemistry, Glycobiology Institute, University of Oxford, Oxford OX1 3QU, UK.
authors.forename: Weston B
authors.initials: WB
authors.lastname: Struwe
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Tiemeyer
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: Lance
authors.initials: L
authors.lastname: Wells
authors.affiliation: Complex Carbohydrate Research Center, University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
authors.forename: William S
authors.initials: WS
authors.lastname: York
authors.affiliation: Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University, School of Medicine, 670 Albany Street, Suite 504, Boston, MA 02118, USA.
authors.forename: Joseph
authors.initials: J
authors.lastname: Zaia
authors.affiliation: Beilstein-Institut, Trakehner Str. 7-9, 60487 Frankfurt am Main, Germany.
authors.forename: Carsten
authors.initials: C
authors.lastname: Kettner
authors.affiliation: Department of Cell and Molecular Biology, The Scripps Research Institute, 10550 N Torrey Pines Road, La Jolla, CA 92037, USA.
authors.forename: James C
authors.initials: JC
authors.lastname: Paulson
authors.affiliation: Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.|Department of Medicine, Glycosciences Laboratory, Imperial College London, Du Cane Road, London W12 0NN, UK.
authors.forename: Ten
authors.initials: T
authors.lastname: Feizi
authors.affiliation: Emory Comprehensive Glycomics Core, Emory University School of Medicine, Atlanta, GA 30322, USA.
authors.forename: David F
authors.initials: DF
authors.lastname: Smith
keywords: MIRAGE; glycan microarrays; glycans; glycobiology; glycomics