title: Potential Renoprotective Effects of Rosemary and Thyme Against Gentamicin Toxicity in Rats.
abstract: Gentamicin (GM) is a drug used commonly against gram-negative bacteria. Its therapeutic use is mainly limited by its nephrotoxicity. This investigation was aimed at evaluating the nephroprotective effects of rosemary (RM; Rosmarinus officinalis) and thyme (TV; Thymus vulgaris) against GM toxicity. Rats were divided into four groups. Normal control group was treated intraperitoneally (i.p.) with saline; positive control group was administered GM 60 mg/kg/day i.p.; RM group was co-administered 8% RM aqueous extract, 10 mL/kg/day, orally with GM; and TV group was co-administered 8% TV aqueous extract orally, 10 mL/kg/day with GM. Both RM and TV groups extracted significantly ameliorated plasma kidney function biomarkers, and reduced malondialdhyde and glucose levels. Meanwhile, RM extract significantly modulated the electrolyte concentration and increased plasma insulin levels as compared with the GM group. However, the aqueous extract of RM showed more pronounced effects than TV extract. Our data were confirmed by histopathological examination and deoxyribonucleic acid (DNA) fragmentation analysis. Deleterious histopathological changes and increased DNA fragmentation induced by GM treatment were markedly controlled by the co-administration of RM and TV. Such renoprotective influence of RM and TV suggests their concurrent supplementation with GM therapy to limit GM toxicity.
authors.affiliation: a Department of Nutrition and Food Sciences , National Research Centre , Dokki, Giza , Egypt.
authors.forename: Amal S
authors.initials: AS
authors.lastname: Abdel-Azeem
authors.affiliation: a Department of Nutrition and Food Sciences , National Research Centre , Dokki, Giza , Egypt.
authors.forename: Amany M
authors.initials: AM
authors.lastname: Hegazy
authors.affiliation: b Department of Research on Children with Special Needs , National Research Centre , Dokki, Giza , Egypt.
authors.forename: Hala M
authors.initials: HM
authors.lastname: Zeidan
authors.affiliation: c Department of Environmental and Occupational Medicine , National Research Centre , Dokki, Giza , Egypt.
authors.forename: Khadiga S
authors.initials: KS
authors.lastname: Ibrahim
authors.affiliation: a Department of Nutrition and Food Sciences , National Research Centre , Dokki, Giza , Egypt.
authors.forename: Eman M
authors.initials: EM
authors.lastname: El-Sayed
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000633:toxicity*; D001786:Blood Glucose / Q000187:drug effects; D004359:Drug Therapy, Combination; D005839:Gentamicins / Q000633:toxicity*; D007328:Insulin / Q000097:blood; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D007677:Kidney Function Tests; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008517:Phytotherapy / Q000379:methods*; D010936:Plant Extracts / Q000008:administration & dosage / Q000494:pharmacology*; D020011:Protective Agents / Q000008:administration & dosage / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D027542:Rosmarinus; D046930:Thymus Plant
chemical_list: D000900:Anti-Bacterial Agents; D001786:Blood Glucose; D005839:Gentamicins; D007328:Insulin; D010936:Plant Extracts; D020011:Protective Agents; D008315:Malondialdehyde
keywords: Rosmarinus officinatis; Thymus vulgaris; gentamicin; kidney; oxidative stress