title: Disruption of type 3 adenylyl cyclase expression in the hypothalamus leads to obesity.
abstract: Evidence from human studies and transgenic mice lacking the type 3 adenylyl cyclase (AC3) indicates that AC3 plays a role in the regulation of body weight. It is unknown in which brain region AC3 exerts such an effect. We examined the role of AC3 in the hypothalamus for body weight control using a floxed AC3 mouse strain. Here, we report that AC3 flox/flox mice became obese after the administration of AAV-CRE-GFP into the hypothalamus. Both male and female AC3 floxed mice showed heavier body weight than AAV-GFP injected control mice. Furthermore, mice with selective ablation of AC3 expression in the ventromedial hypothalamus also showed increased body weight and food consumption. Our results indicated that AC3 in the hypothalamus regulates energy balance.
authors.affiliation: Institute of Neurobiology, Institutes of Brain Science and State Key Laboratory of Medical Neurobiology, Fudan University, China; Department of Pharmacology, School of Medicine, University of Washington, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Cao
authors.affiliation: Department of Pharmacology, School of Medicine, University of Washington, USA; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, USA.
authors.forename: Xuanmao
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Pharmacology, School of Medicine, University of Washington, USA.
authors.forename: Yimei
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Pharmacology, School of Medicine, University of Washington, USA.
authors.forename: Daniel R
authors.initials: DR
authors.lastname: Storm
keywords: hypothalamus; obesity; type 3 adenylyl cyclase