title: Magnetically modified bacterial cellulose: A promising carrier for immobilization of affinity ligands, enzymes, and cells.
abstract: Bacterial cellulose (BC) produced by Komagataeibacter sucrofermentans was magnetically modified using perchloric acid stabilized magnetic fluid. Magnetic bacterial cellulose (MBC) was used as a carrier for the immobilization of affinity ligands, enzymes and cells. MBC with immobilized reactive copper phthalocyanine dye was an efficient adsorbent for crystal violet removal; the maximum adsorption capacity was 388mg/g. Kinetic and thermodynamic parameters were also determined. Model biocatalysts, namely bovine pancreas trypsin and Saccharomyces cerevisiae cells were immobilized on MBC using several strategies including adsorption with subsequent cross-linking with glutaraldehyde and covalent binding on previously activated MBC using sodium periodate or 1,4-butanediol diglycidyl ether. Immobilized yeast cells retained approximately 90% of their initial activity after 6 repeated cycles of sucrose solution hydrolysis. Trypsin covalently bound after MBC periodate activation was very stable during operational stability testing; it could be repeatedly used for ten cycles of low molecular weight substrate hydrolysis without loss of its initial activity.
authors.affiliation: Global Change Research Institute, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic.
authors.forename: Eva
authors.initials: E
authors.lastname: Baldikova
authors.affiliation: Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 27, 783 71 Olomouc, Czech Republic.
authors.forename: Kristyna
authors.initials: K
authors.lastname: Pospiskova
authors.affiliation: Department of Chemical Engineering, University of Patras, 26504 Patras, Rio, Greece.
authors.forename: Dimitrios
authors.initials: D
authors.lastname: Ladakis
authors.affiliation: Department of Chemical Engineering, University of Patras, 26504 Patras, Rio, Greece.
authors.forename: Ioannis K
authors.initials: IK
authors.lastname: Kookos
authors.affiliation: Department of Food Science and Human Nutrition, Agricultural University of Athens, Iera Odos 75, Athens 11855, Greece.
authors.forename: Apostolis A
authors.initials: AA
authors.lastname: Koutinas
authors.affiliation: Global Change Research Institute, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic; Department of Nanobiotechnology, Biology Centre, ISB, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic.
authors.forename: Mirka
authors.initials: M
authors.lastname: Safarikova
authors.affiliation: Global Change Research Institute, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic; Regional Centre of Advanced Technologies and Materials, Palacky University, Slechtitelu 27, 783 71 Olomouc, Czech Republic; Department of Nanobiotechnology, Biology Centre, ISB, CAS, Na Sadkach 7, 370 05 Ceske Budejovice, Czech Republic. Electronic address: safarik@nh.cas.cz.
authors.forename: Ivo
authors.initials: I
authors.lastname: Safarik
mesh_terms: D016947:Acetobacteraceae / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D018914:Cells, Immobilized / Q000166:cytology; D002482:Cellulose / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry*; D004800:Enzymes, Immobilized / Q000737:chemistry*; D005840:Gentian Violet / Q000737:chemistry; D007211:Indoles / Q000737:chemistry; D008280:Magnetics; D009942:Organometallic Compounds / Q000737:chemistry; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D012441:Saccharomyces cerevisiae / Q000166:cytology*; D014357:Trypsin / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D004800:Enzymes, Immobilized; D007211:Indoles; D009942:Organometallic Compounds; D011135:Polysaccharides, Bacterial; C015445:copper phthalocyanine; D002482:Cellulose; D014357:Trypsin; D005840:Gentian Violet
keywords: Bacterial cellulose; Copper phthalocyanine; Crystal violet; Komagataeibacter sucrofermentans; Trypsin; Yeast cells