title: Broncho Alveolar Dendritic Cells and Macrophages Are Highly Similar to Their Interstitial Counterparts.
abstract: In human medicine, bronchoalveolar lavage is the main non-traumatic procedure allowing an insight into the respiratory Dendritic Cells (DC) and Macrophages populations. However, it has never been demonstrated in a relevant model that alveolar DC subpopulations were comparable to their interstitial counterparts. In a precedent work we observed that respiratory pig DC and Macrophages were more similar to the human ones than to the mouse ones. In the present work, thanks to our animal model, we were able to collect the rare bronchoalveolar DC and compare them to their interstitial counterparts. We observed that DC presented very similar gene-expression patterns in the alveolar and interstitial compartments, validating the study of human bronchoalveolar DC as surrogate of their interstitium counterparts.
authors.affiliation: Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Maisonnasse
authors.affiliation: Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Elise
authors.initials: E
authors.lastname: Bordet
authors.affiliation: Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Edwige
authors.initials: E
authors.lastname: Bouguyon
authors.affiliation: Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Domaine de Vilvert, Jouy-en-Josas, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bertho
mesh_terms: D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D003713:Dendritic Cells / Q000166:cytology* / Q000378:metabolism; D005434:Flow Cytometry; D015870:Gene Expression; D007413:Intestinal Mucosa / Q000378:metabolism; D007422:Intestines / Q000166:cytology*; D016676:Macrophages, Alveolar / Q000166:cytology* / Q000378:metabolism; D023421:Models, Animal; D013552:Swine