title: Altered expression of Butyrophilin (BTN) and BTN-like (BTNL) genes in intestinal inflammation and colon cancer.
abstract: Several Butyrophilin (BTN) and Btn-like (BTNL) molecules control T lymphocyte responses, and are genetically associated with inflammatory disorders and cancer. In this study, we present a comprehensive expression analysis of human and murineBTNandBTNLgenes in conditions associated with intestinal inflammation and cancer. Using real-time PCR, expression of humanBTNandBTNLgenes was analyzed in samples from patients with ulcerative colitis, irritable bowel syndrome, and colon tumors. Expression of murineBtnandBtnlgenes was examined in mouse models of spontaneous colitis (Muc2-/-) and intestinal tumorigenesis (ApcMin/+). Our analysis indicates a strong association of several of the human genes with ulcerative colitis and colon cancer; while especiallyBTN1A1,BTN2A2,BTN3A3, andBTNL8were significantly altered in inflammation, colonic tumors exhibited significantly decreased levels ofBTNL2,BTNL3,BTNL8, andBTNL9as compared to unaffected tissue. Colonic inflammation inMuc2-/-mice significantly down-regulated the expression of particularlyBtnl1,Btnl4, andBtnl6mRNA, and intestinal polyps derived fromApcMin/+mice displayed altered levels ofBtn1a1,Btn2a2, andBtnl1transcripts. Thus, our data present an association ofBTNandBTNLgenes with intestinal inflammation and cancer and represent a valuable resource for further studies of this gene family.
authors.affiliation: Department of Microbiology and Immunology Institute of Biomedicine University of Gothenburg Gothenburg Sweden.
authors.forename: Cristina
authors.initials: C
authors.lastname: Lebrero-Fernández
authors.affiliation: Department of Microbiology and Immunology Institute of Biomedicine University of Gothenburg Gothenburg Sweden.
authors.forename: Ulf Alexander
authors.initials: UA
authors.lastname: Wenzel
authors.affiliation: Department of Microbiology and Immunology Institute of Biomedicine University of Gothenburg Gothenburg Sweden.
authors.forename: Paulina
authors.initials: P
authors.lastname: Akeus
authors.affiliation: Department of Microbiology and Immunology Institute of Biomedicine University of Gothenburg Gothenburg Sweden.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Internal Medicine and Clinical Nutrition Institute of Medicine University of Gothenburg Gothenburg Sweden.
authors.forename: Hans
authors.initials: H
authors.lastname: Strid
authors.affiliation: Department of Internal Medicine and Clinical NutritionInstitute of MedicineUniversity of GothenburgGothenburgSweden; Center for Functional GI and Motility DisordersUniversity of North CarolinaChapel HillNorth CarolinaUSA.
authors.forename: Magnus
authors.initials: M
authors.lastname: Simrén
authors.affiliation: Department of Surgery Institute of Clinical Sciences University of Gothenburg Gothenburg Sweden.
authors.forename: Bengt
authors.initials: B
authors.lastname: Gustavsson
authors.affiliation: Department of Surgery Institute of Clinical Sciences University of Gothenburg Gothenburg Sweden.
authors.forename: Lars G
authors.initials: LG
authors.lastname: Börjesson
authors.affiliation: Department of Microbiology and Immunology Institute of Biomedicine University of Gothenburg Gothenburg Sweden.
authors.forename: Susanna L
authors.initials: SL
authors.lastname: Cardell
authors.affiliation: Department of Microbiology and ImmunologyInstitute of BiomedicineUniversity of GothenburgGothenburgSweden; Department of Internal Medicine and Clinical NutritionInstitute of MedicineUniversity of GothenburgGothenburgSweden; School of Health and EducationUniversity of SkövdeSkövdeSweden.
authors.forename: Lena
authors.initials: L
authors.lastname: Öhman
authors.affiliation: Department of Microbiology and Immunology Institute of Biomedicine University of Gothenburg Gothenburg Sweden.
authors.forename: Marianne
authors.initials: M
authors.lastname: Quiding-Järbrink
authors.affiliation: Department of Microbiology and Immunology Institute of Biomedicine University of Gothenburg Gothenburg Sweden.
authors.forename: Anna
authors.initials: A
authors.lastname: Bas-Forsberg
mesh_terms: D000818:Animals; D000071797:Butyrophilins / Q000378:metabolism*; D003093:Colitis, Ulcerative / Q000378:metabolism; D003106:Colon; D003110:Colonic Neoplasms / Q000378:metabolism*; D006801:Humans; D007249:Inflammation / Q000378:metabolism*; D007413:Intestinal Mucosa; D051379:Mice; D008810:Mice, Inbred C57BL; D013601:T-Lymphocytes
chemical_list: C408263:BTNL2 protein, human; C558164:Btnl1 protein, mouse; D000071797:Butyrophilins
keywords: Butyrophilin (Btn)‐like (Btnl); colon cancer; immune regulation; intestinal inflammation; irritable bowel syndrome (IBS); ulcerative colitis (UC)