title: Scutellarin-graft cationic β-cyclodextrin-polyrotaxane: Synthesis, characterization and DNA condensation.
abstract: As a prerequisite of gene delivery in living cells, DNA condensation has attracted more and more attention. In order to improve the efficiencies of polyamine-β-cyclodextrin-based cationic polyrotaxanes (PR-EDA and PR-DETA) as DNA condensation materials, we have designed and prepared two novel scutellarin-grafted cationic polyrotaxanes (PR-EDA-SCU and PR-DETA-SCU), in which scutellarins (SCU), the planar molecules, were conjugated on the cyclodextrin molecules of PR-EDA and PR-DETA. These materials were characterized by 1D and 2D NMR, XRD, TG and DSC. The electrophoresis assays showed that pDNA condensation efficiencies of PR-EDA and PR-DETA were better than that of PR-EDA and PR-DETA. The complexes of PR-EDA, PR-DETA, PR-EDA-SCU and PR-DETA-SCU with pDNA were further investigated by zeta potential and atomic force microscopy analysis. The results indicated that the planar structure of SCU played an important role in improvement of pDNA condensation efficiencies of PR-EDA-SCU and PR-DETA-SCU. The satisfactory pDNA condensation abilities of PR-EDA-SCU and PR-DETA-SCU could be helpful in designing non-viral gene delivery vectors to control gene expression and delivery.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Qin
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Ma
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China.
authors.forename: Xiali
authors.initials: X
authors.lastname: Liao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, PR China. Electronic address: yangbo6910@sina.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Yang
mesh_terms: D003505:Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry; D004247:DNA / Q000737:chemistry*; D018014:Gene Transfer Techniques; D020442:Poloxamer / Q000138:chemical synthesis / Q000737:chemistry; D043862:Rotaxanes / Q000138:chemical synthesis / Q000737:chemistry; D012697:Serine Endopeptidases / Q000138:chemical synthesis / Q000737:chemistry; D047392:beta-Cyclodextrins / Q000138:chemical synthesis / Q000737:chemistry
chemical_list: D003505:Cyclodextrins; D043862:Rotaxanes; D047392:beta-Cyclodextrins; C475673:polyrotaxane; D020442:Poloxamer; D004247:DNA; D012697:Serine Endopeptidases; C051692:scutelarin
keywords: Cationic polyrotaxane; Characterization; Cyclodextrin; DNA condensation; Scutellarin; Synthesis