title: ABI5 Is a Regulator of Seed Maturation and Longevity in Legumes.
abstract: The preservation of our genetic resources and production of high-quality seeds depends on their ability to remain viable and vigorous during storage. In a quantitative trait locus analysis on seed longevity in Medicago truncatula, we identified the bZIP transcription factor ABSCISIC ACID INSENSITIVE5 (ABI5). Characterization of Mt-abi5 insertion mutant seeds revealed that both the acquisition of longevity and dormancy were severely impaired. Using transcriptomes of developing Mt-abi5 seeds, we created a gene coexpression network and revealed ABI5 as a regulator of gene modules with functions related to raffinose family oligosaccharide (RFO) metabolism, late embryogenesis abundant (LEA) proteins, and photosynthesis-associated nuclear genes (PhANGs). Lower RFO contents in Mt-abi5 seeds were linked to the regulation of SEED IMBIBITION PROTEIN1 Proteomic analysis confirmed that a set of LEA polypeptides was reduced in mature Mt-abi5 seeds, whereas the absence of repression of PhANG in mature Mt-abi5 seeds was accompanied by chlorophyll and carotenoid retention. This resulted in a stress response in Mt-abi5 seeds, evident from an increase in α-tocopherol and upregulation of genes related to programmed cell death and protein folding. Characterization of abi5 mutants in a second legume species, pea (Pisum sativum), confirmed a role for ABI5 in the regulation of longevity, seed degreening, and RFO accumulation, identifying ABI5 as a prominent regulator of late seed maturation in legumes.
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Julia
authors.initials: J
authors.lastname: Zinsmeister
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: David
authors.initials: D
authors.lastname: Lalanne
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Terrasson
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Emilie
authors.initials: E
authors.lastname: Chatelain
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Vandecasteele
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Benoit Ly
authors.initials: BL
authors.lastname: Vu
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Cécile
authors.identifier: 0000-0001-7654-0132
authors.initials: C
authors.lastname: Dubois-Laurent
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Emmanuel
authors.identifier: 0000-0003-4384-3107
authors.initials: E
authors.lastname: Geoffriau
authors.affiliation: Agroécologie, UMR1347, INRA, BP 86510, F-21000 Dijon, France.
authors.forename: Christine Le
authors.identifier: 0000-0002-3204-3537
authors.initials: CL
authors.lastname: Signor
authors.affiliation: Institute of Plant Sciences Paris-Saclay, INRA, CNRS, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, 91405 Orsay, France.
authors.forename: Marion
authors.identifier: 0000-0002-1673-3095
authors.initials: M
authors.lastname: Dalmais
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Gutbrod
authors.affiliation: Institute of Molecular Physiology and Biotechnology of Plants, University of Bonn, 53115 Bonn, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Dörmann
authors.affiliation: Agroécologie, UMR1347, INRA, BP 86510, F-21000 Dijon, France.
authors.forename: Karine
authors.identifier: 0000-0002-2343-1941
authors.initials: K
authors.lastname: Gallardo
authors.affiliation: Institute of Plant Sciences Paris-Saclay, INRA, CNRS, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, 91405 Orsay, France.
authors.forename: Abdelhafid
authors.initials: A
authors.lastname: Bendahmane
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France.
authors.forename: Julia
authors.identifier: 0000-0002-1457-764X
authors.initials: J
authors.lastname: Buitink
authors.affiliation: IRHS, Agrocampus Ouest, INRA, Université d'Angers, SFR 4207 QUASAV, 49071 Beaucouzé, France olivier.leprince@agrocampus-ouest.fr.
authors.forename: Olivier
authors.identifier: 0000-0003-1414-8690
authors.initials: O
authors.lastname: Leprince
mesh_terms: D002338:Carotenoids / Q000378:metabolism; D002734:Chlorophyll / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D046913:Medicago truncatula / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D018532:Pisum sativum / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D012639:Seeds / Q000235:genetics / Q000378:metabolism* / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D002734:Chlorophyll; D002338:Carotenoids