title: Development of the method for identification of selected populations of torpedo scad, Megalaspis cordyla (Linnaeus, 1758), using microsatellite DNA analyses. CELFISH project - Part 4.
abstract: Catch and consumption of torpedo scad (Megalaspis cordyla) over the western Indian Ocean, but also the western Pacific from Japan to Australia is constantly increasing. Taking into account the degree of exploitation and missing information on the population structure of torpedo scad stocks it is crucial to provide population data. The analysis included individuals obtained in 2012 and 2013 from local markets in Madagascar, Tanzania, Vietnam and Cambodia and after successful DNA extraction fragment of the nuclear rhodopsin gene (RH1) and 9 microsatellite regions (SSRs) were amplified and analysed. Based on the obtained results it was found that there was no 100% overlap between the compared RH1 sequences and those from GenBank. In the case of the studied SSRs, the results allowed the initial characterisation and assessment of the genetic diversity of populations. Moreover, population assignment test distinguished the studied populations into two geographically distant subpopulations.
authors.affiliation: Department of Aquaculture, Faculty of Food Science and Fisheries, West Pomeranian University of Technology, Szczecin, Poland. Electronic address: jkempter@zut.edu.pl.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Kempter
authors.affiliation: Department of Aquaculture, Faculty of Food Science and Fisheries, West Pomeranian University of Technology, Szczecin, Poland. Electronic address: mkielpinski@zut.edu.pl.
authors.forename: Maciej
authors.initials: M
authors.lastname: Kielpinski
authors.affiliation: Department of Aquaculture, Faculty of Food Science and Fisheries, West Pomeranian University of Technology, Szczecin, Poland. Electronic address: rpanicz@zut.edu.pl.
authors.forename: Remigiusz
authors.initials: R
authors.lastname: Panicz
authors.affiliation: Department of Genetics and Animal Breeding, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology, Szczecin, Poland. Electronic address: Kaja.Pruffer@zut.edu.pl.
authors.forename: Kaja
authors.initials: K
authors.lastname: Prüffer
authors.affiliation: Department of Aquaculture, Faculty of Food Science and Fisheries, West Pomeranian University of Technology, Szczecin, Poland. Electronic address: skeszka@zut.edu.pl.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Keszka
mesh_terms: D000818:Animals; D004247:DNA; D005399:Fishes / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D019156:Indian Ocean; D018895:Microsatellite Repeats
chemical_list: D004247:DNA
keywords: Genetic traceability; Microsatellite; Seafood authentication; Seafood counterfeiting