title: Association of oxidative stress and DNA damage with grafting time in patients with multiple myeloma and lymphoma submitted to autologous hematopoietic stem cell transplantation.
abstract: The aim of the study was to investigate the association between oxidative stress and DNA damage with grafting time in patients submitted to autologous hematopoietic stem-cell transplantation (HSCT). The study included 37 patients submitted to autologous HSCT diagnosed with Multiple Myeloma (MM) and lymphoma (Hodgkin's and non-Hodgkin's). Biomarkers of oxidative stress and DNA damage index (DI) were performed at baseline (pre-CR) of the disease and during the conditioning regimen (CR), one day after the HSCT, ten days after HSCT and twenty days after HSCT, as well as in the control group consisting of 30 healthy individuals. The outcomes showed that both groups of patients had an hyperoxidative state with high DI when compared to baseline and to the control group and that the CR exacerbated this condition. However, after the follow-up period of the study, this picture was re-established to the baseline levels of each pathology. The study patients with MM showed a mean grafting time of 10.75 days (8 to 13 days), with 10.15 days (8 to 15 days) for the lymphoma patients. In patients with MM, there was a negative correlation between the grafting time and the basal levels of GPx (r = -0.54; p = 0.034), indicating that lower levels of this important enzyme are associated with a longer grafting time. For the DI, the correlation was a positive one (r = 0.529; p = 0.030). In the group with lymphoma, it was observed that the basal levels of NOx were positively correlated with grafting time (r = 0.4664, p = 0.032). The data indicate the potential of these biomarkers as predictors of toxicity and grafting time in patients with MM and Lymphomas submitted to autologous HSCT.
authors.affiliation: Laboratory of Hemoglobinopathy Research and Genetics of Hematologic Diseases, Universidade Federal do Ceará, Brazil.
authors.forename: Thayna Nogueira Dos
authors.initials: TN
authors.lastname: Santos
authors.affiliation: Hematopoietic Cell Transplantation Service, Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Brazil.
authors.forename: Fernando Barroso
authors.initials: FB
authors.lastname: Duarte
authors.affiliation: Laboratory of Hemoglobinopathy Research and Genetics of Hematologic Diseases, Universidade Federal do Ceará, Brazil.
authors.forename: Pedro Aurio
authors.initials: PA
authors.lastname: Maia Filho
authors.affiliation: Laboratory of Hemoglobinopathy Research and Genetics of Hematologic Diseases, Universidade Federal do Ceará, Brazil.
authors.forename: Talyta Ellen de Jesus Dos
authors.initials: TE
authors.lastname: Santos
authors.affiliation: Laboratory of Hemoglobinopathy Research and Genetics of Hematologic Diseases, Universidade Federal do Ceará, Brazil.
authors.forename: Maritza Cavalcante
authors.initials: MC
authors.lastname: Barbosa
authors.affiliation: Laboratory of Hemoglobinopathy Research and Genetics of Hematologic Diseases, Universidade Federal do Ceará, Brazil.
authors.forename: Tarcísio Paulo de
authors.initials: TP
authors.lastname: Almeida Filho
authors.affiliation: Department of Physiology and Pharmacology, Universidade Federal do Ceará, Brazil.
authors.forename: Bruno Coelho
authors.initials: BC
authors.lastname: Cavalcanti
authors.affiliation: Department of Surgery, Universidade Federal do Ceará, Brazil.
authors.forename: Paulo Roberto Leitão de
authors.initials: PR
authors.lastname: Vasconcelos
authors.affiliation: Laboratory of Hemoglobinopathy Research and Genetics of Hematologic Diseases, Universidade Federal do Ceará, Brazil.
authors.forename: Luana Leticia
authors.initials: LL
authors.lastname: Dutra
authors.affiliation: Hematopoietic Cell Transplantation Service, Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Brazil.
authors.forename: Germison Silva
authors.initials: GS
authors.lastname: Lopes
authors.affiliation: Hematopoietic Cell Transplantation Service, Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Brazil.
authors.forename: Franciclea Oliveira
authors.initials: FO
authors.lastname: Costa
authors.affiliation: Hematopoietic Cell Transplantation Service, Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Brazil.
authors.forename: João Paulo Vasconcelos
authors.initials: JP
authors.lastname: Leitão
authors.affiliation: Hematopoietic Cell Transplantation Service, Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Brazil.
authors.forename: Jacques
authors.initials: J
authors.lastname: KauFman
authors.affiliation: Hematopoietic Cell Transplantation Service, Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Brazil.
authors.forename: Beatriz Stella Pitombeira
authors.initials: BS
authors.lastname: AraúJo
authors.affiliation: Hematopoietic Cell Transplantation Service, Hospital Universitário Walter Cantídio, Universidade Federal do Ceará, Brazil.
authors.forename: Karine Sampaio Nunes
authors.initials: KS
authors.lastname: Barroso
authors.affiliation: Department of Clinical and Toxicological Analyses, Universidade Federal do Ceará, Brazil.
authors.forename: Romélia Pinheiro Gonçalves
authors.initials: RP
authors.lastname: Lemes
mesh_terms: D000704:Analysis of Variance; D015415:Biomarkers; D016022:Case-Control Studies; D004249:DNA Damage / Q000502:physiology*; D005260:Female; D018380:Hematopoietic Stem Cell Transplantation / Q000379:methods*; D006801:Humans; D008223:Lymphoma / Q000235:genetics / Q000378:metabolism / Q000601:surgery*; D008297:Male; D008315:Malondialdehyde / Q000032:analysis; D009101:Multiple Myeloma / Q000235:genetics / Q000378:metabolism / Q000601:surgery*; D018384:Oxidative Stress / Q000502:physiology*; D012016:Reference Values; D013997:Time Factors; D014182:Transplantation, Autologous / Q000379:methods; D016896:Treatment Outcome
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde