title: The Promotion of Neural Regeneration in A Rat Facial Nerve Crush Injury Model Using Collagen-Binding NT-3.
abstract: Traumatic facial nerve injury, an important cause of facial paralysis, has a number of adverse effects, including facial muscle dysfunction and facial asymmetry. It has been demonstrated in our previous work that native human NT-3 fused with a collagen-binding domain (CBD-NT-3) could bind to collagen, specifically to exert neurotrophic effects, promoting axonal regeneration. To evaluate the effect of CBD-NT-3 in inducing facial nerve regeneration and functional recovery, the differing effects of CBD-NT-3 and native neurotrophin-3 (NAT-NT-3) were observed using the results of facial nerve functional recovery, electrophysiological testing, and axonal and myelin changes in a rat model of facial nerve crush injury. The rats were injected in the epineurium in crushed fibers of the facial nerve with CBD-NT-3, NAT-NT-3, and PBS respectively. After 4 weeks, the CBD-NT-3 group demonstrated significantly more ordered growth of axons and nerve functional recovery than the NAT-NT-3 group. The results suggest that CBD-NT-3 considerably enhances facial nerve regeneration and functional recovery.
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Handong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Ni
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
authors.forename: Sufang
authors.initials: S
authors.lastname: Han
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Xu
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Yuan
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China.
authors.forename: Tiansheng
authors.initials: T
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China njneurosurgery@hotmail.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.affiliation: Department of Neurosurgery, Nanjing Drum Tower Hospital, Medical School of Nanjing University, Nanjing, Jiangsu, China njneurosurgery@163.com.
authors.forename: Weibang
authors.initials: W
authors.lastname: Liang
authors.affiliation: State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China jwdaichina@163.com.
authors.forename: Jianwu
authors.initials: J
authors.lastname: Dai
mesh_terms: D000200:Action Potentials / Q000187:drug effects; D000818:Animals; D001369:Axons / Q000187:drug effects / Q000473:pathology; D003094:Collagen / Q000378:metabolism*; D000071576:Crush Injuries / Q000188:drug therapy* / Q000503:physiopathology* / Q000601:surgery; D004195:Disease Models, Animal; D005109:Extracellular Matrix / Q000378:metabolism; D005154:Facial Nerve / Q000187:drug effects / Q000503:physiopathology* / Q000648:ultrastructure; D006801:Humans; D007150:Immunohistochemistry; D009416:Nerve Regeneration / Q000187:drug effects; D020933:Neurotrophin 3 / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use*; D011485:Protein Binding / Q000187:drug effects; D000072417:Protein Domains; D017207:Rats, Sprague-Dawley; D020127:Recovery of Function
chemical_list: D020933:Neurotrophin 3; D003094:Collagen
keywords: Facial nerve injury; collagen-binding domain; nerve regeneration; neurotrophin-3