title: An Environmentally Benign Antimicrobial Coating Based on a Protein Supramolecular Assembly.
abstract: The use of antimicrobial materials, for example, silver nanoparticles, has been a cause for concern because they often exert an adverse effect on environmental and safety during their preparation and use. In this study, we report a class of green antimicrobial coating based on a supramolecular assembly of a protein extracted from daily food, without the addition of any other hazardous agents. It is found that a self-assembled nanofilm by mere hen egg white lysozyme has durable in vitro and in vivo broad-spectrum antimicrobial efficacy against Gram-positive/negative and fungi. Such enhanced antimicrobial capability over native lysozyme is attributed to a synergistic combination of positive charge and hydrophobic amino acid residues enriched on polymeric aggregates in the lysozyme nanofilm. Accompanied with high antimicrobial activity, this protein-based PTL material simultaneously exhibits the integration of multiple functions including antifouling, antibiofilm, blood compatibility, and low cytotoxicity due to the existence of surface hydration effect. Moreover, the bioinspired adhesion mediated by the amyloid structure contained in the nanofilm induces robust transfer and self-adhesion of the material onto virtually arbitrary substrates by a simple one-step aqueous coating or solvent-free printing in 1 min, thereby allowing an ultrafast route into practical implications for surface-functionalized commodity and biomedical devices. Our results demonstrate that the application of pure proteinaceous substance may afford a cost-effective green biomaterial that has high antimicrobial activity and low environmental impact.
authors.affiliation: Key Laboratory of Applied Surface and Colloids Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University , Xi'an 710119, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Gu
authors.affiliation: Center for Biomedical Engineering and Regenerative Medicine, Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an 710049, China.
authors.forename: Yajuan
authors.initials: Y
authors.lastname: Su
authors.affiliation: College of Biomedical Engineering, Chongqing University , Chongqing 400044, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Center for Biomedical Engineering and Regenerative Medicine, Frontier Institute of Science and Technology, Xi'an Jiaotong University , Xi'an 710049, China.|Key Laboratory of Flexible Electronics (KLOFE) and Institute of Advanced Materials (IAM), Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), Nanjing Tech University (NanjingTech) , Nanjing 210009, China.
authors.forename: Peng
authors.identifier: 0000-0002-5876-2177
authors.initials: P
authors.lastname: Li
authors.affiliation: Key Laboratory of Applied Surface and Colloids Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University , Xi'an 710119, China.
authors.forename: Peng
authors.identifier: 0000-0002-0463-1024
authors.initials: P
authors.lastname: Yang
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry*; D002645:Chickens; D011108:Polymers; D012834:Silver
chemical_list: D000890:Anti-Infective Agents; D011108:Polymers; D012834:Silver
keywords: antimicrobial; biocompatibility; lysozyme; protein phase transition; surface coating