title: Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, tissue distribution and excretion in the study of anemoside B4, a novel antiviral agent candidate, in rats.
abstract: A simple, sensitive and reliable LC-MS/MS method was developed and validated for the quantification of anemoside B4, a potential antiviral constituent isolated from Pulsatilla chinensis in rat plasma, tissue, bile, urine and feces. All biological samples were prepared by protein precipitation method, and ginsenoside-Rg1 was chosen as the internal standard (IS). The analyte and IS were separated using a C18column (2.1 × 50 mm, 1.8 μm) and a mobile phase consisting of 0.1% formic acid in water (v/v) and acetonitrile running at a flow rate of 0.2 mL/min for 5 min. The multiple reaction monitoring transitions were monitored at m/z 1219.5-749.5 for anemoside B4 and 845.4-637.4 for ginsenoside-Rg1 in electrospray ionization negative mode. The calibration curve was linear in the range of 10-2000 ng/mL for all biological matrices with a lower limit of quantification of 10 ng/mL. The validated method was successfully applied to a pharmacokinetics, tissue distribution and excretion study. These preclinical data will be beneficial for further development of anemoside B4 in future studies.
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Mingyu
authors.initials: M
authors.lastname: He
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Mingzhen
authors.initials: M
authors.lastname: He
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Ting
authors.initials: T
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, People's Republic of China.
authors.forename: Junmao
authors.initials: J
authors.lastname: Li
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, People's Republic of China.
authors.forename: Yulin
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.|State Key Laboratory of Innovative Drug and Efficient Energy-Saving Pharmaceutical Equipment, Nanchang, People's Republic of China.
authors.forename: Shilin
authors.initials: S
authors.lastname: Yang
mesh_terms: D000818:Animals; D000998:Antiviral Agents / Q000493:pharmacokinetics* / Q000652:urine; D002138:Calibration; D002853:Chromatography, Liquid / Q000379:methods*; D057230:Limit of Detection; D051381:Rats; D012015:Reference Standards; D012503:Saponins / Q000493:pharmacokinetics*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000998:Antiviral Agents; D012503:Saponins; C000620474:anemoside B4
keywords: LC-MS/MS; anemoside B4; excretion; pharmacokinetics; tissue distribution