title: Characterization of a triple-recombinant, reassortant rotavirus strain from the Dominican Republic.
abstract: We report the genome of a novel human triple-recombinant G4P[6-8_R] mono-reassortant strain identified in a stool sample from the Dominican Republic during routine facility-based rotavirus strain surveillance. The strain was designated as RVA/Human-wt/DOM/2013840364/2013/G4P[6-8_R], with a genomic constellation of G4-P[6-8_R]-I1-R1-C1-M1-(A1-A8_R)-N1-(T1-T7_R)-E1-H1. Recombinant gene segments NSP1 and NSP3 were generated as a result of recombination between genogroup 1 rotavirus A1 human strain and a genotype A8 porcine strain and between genogroup 1 rotavirus T1 human strain and a genotype T7 bovine strain, respectively. Analyses of the RNA secondary structures of gene segment VP4, NSP1 and NSP3 showed that all the recombinant regions appear to start in a loop (single-stranded) region and terminate in a stem (double-stranded) structure. Also, the VP7 gene occupied lineage VII within the G4 genotypes consisting of mostly porcine or porcine-like G4 strains, suggesting the occurrence of reassortment. The remaining gene segments clustered phylogenetically with genogroup 1 strains. This exchange of whole or partial genetic materials between rotaviruses by recombination and reassortment contributes directly to their diversification, adaptation and evolution.
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Mathew D
authors.initials: MD
authors.lastname: Esona
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Sunando
authors.initials: S
authors.lastname: Roy
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Kunchala
authors.initials: K
authors.lastname: Rungsrisuriyachai
authors.affiliation: Hospital Infantil Dr Robert Reid Cabral, Santo Domingo, Dominican Republic.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Sanchez
authors.affiliation: Hospital Infantil Dr Robert Reid Cabral, Santo Domingo, Dominican Republic.
authors.forename: Lina
authors.initials: L
authors.lastname: Vasquez
authors.affiliation: Hospital Infantil Dr Robert Reid Cabral, Santo Domingo, Dominican Republic.
authors.forename: Virgen
authors.initials: V
authors.lastname: Gomez
authors.affiliation: Yale School of Medicine, New Haven, CT, USA.
authors.forename: Lourdes Aviles
authors.initials: LA
authors.lastname: Rios
authors.affiliation: Centers for Disease Control and Prevention, Atlanta, GA, USA.
authors.forename: Michael D
authors.initials: MD
authors.lastname: Bowen
authors.affiliation: Yale School of Medicine, New Haven, CT, USA.
authors.forename: Marietta
authors.initials: M
authors.lastname: Vazquez
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000818:Animals; D002417:Cattle / Q000821:virology; D004293:Dominican Republic; D062665:Epidemiological Monitoring; D019143:Evolution, Molecular; D005243:Feces / Q000821:virology; D005759:Gastroenteritis / Q000662:veterinary / Q000821:virology*; D014644:Genetic Variation; D016679:Genome, Viral; D023281:Genomics; D005838:Genotype; D006801:Humans; D005810:Multigene Family; D009690:Nucleic Acid Conformation; D010802:Phylogeny; D012367:RNA, Viral / Q000737:chemistry / Q000235:genetics; D016865:Reassortant Viruses / Q000145:classification / Q000235:genetics*; D011995:Recombination, Genetic; D012401:Rotavirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D012400:Rotavirus Infections / Q000821:virology*; D017422:Sequence Analysis, DNA; D013552:Swine / Q000821:virology; D017361:Viral Nonstructural Proteins / Q000235:genetics
chemical_list: C082740:NSP3 protein, Rotavirus; D012367:RNA, Viral; D017361:Viral Nonstructural Proteins; C104282:nsp1 protein, Rotavirus