title: Pressurised liquid extraction and liquid chromatography-high resolution mass spectrometry to determine high-intensity sweeteners in fish samples.
abstract: An analytical method based on pressurised liquid extraction (PLE) followed by liquid chromatography-high resolution mass spectrometry (Orbitrap) was developed for the simultaneous determination of ten high-intensity sweeteners in fish samples. As the method was developed, the different PLE parameters were optimised and different clean-up strategies were evaluated, of which in-cell clean-up using alumina and on-cell clean-up with hexane were the most effective. PLE recoveries were between 43% and 94%. The method quantification limits were between 12.5ngg-1dry weight (d.w.) and 250ngg-1(d.w.) and the method detection limits between 2.5ngg-1(d.w.) and 125ngg-1(d.w.). Intra-day precision and inter-day precision were below 16% and 25%, respectively. Fish samples from different species were analysed and, saccharin was found below its method quantification limit in the species Scomber scombrus (Atlantic mackerel).
authors.affiliation: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Sescelades Campus, Marcel·lí Domingo s/n, 43007 Tarragona, Spain.
authors.forename: Mireia
authors.initials: M
authors.lastname: Núñez
authors.affiliation: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Sescelades Campus, Marcel·lí Domingo s/n, 43007 Tarragona, Spain.
authors.forename: Francesc
authors.initials: F
authors.lastname: Borrull
authors.affiliation: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Sescelades Campus, Marcel·lí Domingo s/n, 43007 Tarragona, Spain. Electronic address: eva.pocurull@urv.cat.
authors.forename: Eva
authors.initials: E
authors.lastname: Pocurull
authors.affiliation: Department of Analytical Chemistry and Organic Chemistry, Universitat Rovira i Virgili, Sescelades Campus, Marcel·lí Domingo s/n, 43007 Tarragona, Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Fontanals
mesh_terms: D000818:Animals; D002853:Chromatography, Liquid; D005399:Fishes; D005504:Food Analysis / Q000379:methods*; D057230:Limit of Detection; D013058:Mass Spectrometry; D064306:Non-Nutritive Sweeteners / Q000032:analysis*; D015203:Reproducibility of Results; D012439:Saccharin / Q000032:analysis; D053719:Tandem Mass Spectrometry; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D064306:Non-Nutritive Sweeteners; D014874:Water Pollutants, Chemical; D012439:Saccharin
keywords: Fish samples; Liquid chromatography-high resolution mass spectrometry; Pressurised liquid extraction; Sweeteners