title: Novel genes in brain tissues of EAE-induced normal and obese mice: Upregulation of metal ion-binding protein genes in obese-EAE mice.
abstract: Experimental autoimmune encephalomyelitis (EAE) is an inflammatory autoimmune disease of the central nervous system resulting from degeneration of the myelin sheath. This study is aimed to identify differentially expressed genes (DEGs) in the brain of EAE-induced normal diet (ND) mice and high-fat diet (HFD)-induced obese mice, and to identify novel genes responsible for elucidating the mechanism of the disease. Purified mRNA samples from the brain tissue were analyzed for gene microarray and validated by real-time RT-PCR. DEGs were identified if significant changes greater than 1.5-fold or less than 0.66-fold were observed (p<0.05). Pathway construction and functional categorization were performed using the Kyoto encyclopedia of genes and genomes pathways and gene ontology (GO) analysis. HFD-EAE mice showed more severe disease symptoms than ND-EAE mice. From GO study, fold changes of HFD-EAE to ND-EAE genes indicated that the genes were significantly associated to the pathways related with the immune response, antigen presentation, and complement activation. The genes related with metal ion-binding proteins were upregulated in HFD-EAE and ND-EAE mice. Upregulation of Cul9, Mast2, and C4b expression is significantly higher in HFD-EAE mice than ND-EAE mice. Cul9, Mast2, C4b, Psmb8, Ly86, and Ms4a6d were significantly upregulated in both ND- and HFD-EAE mice. Fcgr4, S3-12, Gca, and Zdhhc4 were upregulated only in ND-EAE, and Xlr4b was upregulated only in HFD-EAE mice. And significant upregulated genes of metal ion-binding proteins (Cul9 and Mast2) were observed in HFD-EAE mice.
authors.affiliation: Toxicology Lab., Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Department of Biological Chemistry, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Mahbub
authors.initials: M
authors.lastname: Hasan
authors.affiliation: Toxicology Lab., Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Department of Biological Chemistry, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Seo
authors.affiliation: Toxicology Lab., Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Department of Biological Chemistry, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Khandoker Asiqur
authors.initials: KA
authors.lastname: Rahaman
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Hophil
authors.initials: H
authors.lastname: Min
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Ki Hun
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Ju-Hyung
authors.initials: JH
authors.lastname: Park
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Changmin
authors.initials: C
authors.lastname: Sung
authors.affiliation: Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea.
authors.forename: Junghyun
authors.initials: J
authors.lastname: Son
authors.affiliation: Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Department of Biological Chemistry, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Department of Biological Chemistry, Korea University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Byung Hwa
authors.initials: BH
authors.lastname: Jung
authors.affiliation: Department of Pathology, Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea.
authors.forename: Won Sang
authors.initials: WS
authors.lastname: Park
authors.affiliation: Toxicology Lab., Doping Control Center, Korea Institute of Science and Technology, Seoul 02792, Republic of Korea; Department of Biological Chemistry, Korea University of Science and Technology, Daejeon 34113, Republic of Korea. Electronic address: oskwon@kist.re.kr.
authors.forename: Oh-Seung
authors.initials: OS
authors.lastname: Kwon
mesh_terms: D000818:Animals; D001835:Body Weight; D001921:Brain / Q000378:metabolism*; D016000:Cluster Analysis; D059305:Diet, High-Fat / Q000009:adverse effects; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000150:complications / Q000378:metabolism*; D005786:Gene Expression Regulation; D008297:Male; D008810:Mice, Inbred C57BL; D008820:Mice, Obese; D046228:Microarray Analysis; D009765:Obesity / Q000150:complications / Q000378:metabolism*; D012333:RNA, Messenger / Q000378:metabolism; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D012720:Severity of Illness Index; D015854:Up-Regulation
chemical_list: D012333:RNA, Messenger
keywords: diet-induced obesity; experimental autoimmune encephalomyelitis; gene microarray; metal ion-binding protein genes