title: Multiple signal amplified electrochemiluminescent immunoassay for brombuterol detection using gold nanoparticles and polyamidoamine dendrimers-silver nanoribbon.
abstract: Electrochemiluminescent (ECL) immunosensor with multiple signal amplification was designed based on gold nanoparticles (AuNPs), polyamidoamine dendrimers (PAMAM) and silver-cysteine hybrid nanoribbon (SNR). Low toxic l-cysteine capped CdSe QDs was chosen as the ECL signal probe. To verify the proposed ultrasensitive ECL immunosensor for β-adrenergic agonists (β-AA), we detected Brombuterol (Brom) as a proof-of-principle analyte. Therein, AuNPs as the substrate can simplify the experiment process, accelerate the electron transfer rate, and carry more coating antigen (Ag-OVA) to enlarge ECL signal. On one hand, SNR on the surface of electrode can avoid the aggregation of AuNPs, and SNR-PAMAM-AuNPs also can be acted as a good accelerator for electron transfer. On the other hand, PAMAM (16 -NH2) functionalized SNR (SNR-PAMAM) with numerous amino groups could be employed to bond abundant actived QDs to further amplify ECL signal. The new immunosensor can offer a simple, reliable, rapid, and selective detection for Brom, which have a dynamic range of 0.005-700 ng mL-1with a low detection limit at 1.5 pg mL-1. The proposed biosensor will extend the application of nanomaterials in ECL immunoassays and open a new road for the detection of Brom and other β-AA in the future.
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Dong
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Liuyi
authors.initials: L
authors.lastname: Hu
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China.
authors.forename: Kang
authors.initials: K
authors.lastname: Zhao
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China. Electronic address: denganping@suda.edu.cn.
authors.forename: Anping
authors.initials: A
authors.lastname: Deng
authors.affiliation: The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China. Electronic address: lijgsd@suda.edu.cn.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Li
mesh_terms: D000814:Aniline Compounds / Q000032:analysis*; D050091:Dendrimers / Q000737:chemistry*; D055664:Electrochemical Techniques; D004983:Ethanolamines / Q000032:analysis*; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D011073:Polyamines / Q000737:chemistry*; D012834:Silver / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000814:Aniline Compounds; D050091:Dendrimers; D004983:Ethanolamines; C531249:Poly(amidoamine); D011073:Polyamines; D012834:Silver; C107573:brombuterol; D006046:Gold
keywords: Amplification strategy; Brombuterol; CdSe QDs; Electrochemiluminescent immunosensor; Gold nanoparticles; Silver-cysteine hybrid nanoribbon