title: Determination of the microbial community features of Haemaphysalis flava in different developmental stages by high-throughput sequencing.
abstract: In this study, we analyzed the in vivo microbial community structure and diversity differences of Haemaphysalis flava (H. flava) in four developmental stages (egg, larva, nymph, and adult) to determine which bacterial genera could be propagated through transovarial transmission and transmitted by transstadial transmission during different developmental stages of H. flava. Paired-end sequencing of the V3 region of the 16S ribosomal DNA (16S rDNA) of H. flava in four developmental stage samples was conducted using the Illumina MiSeq sequencing platform. The following operational taxonomic units (OTUs) of H. flava were obtained during the four developmental stages: 89 of egg, 111 of larva, 104 of nymph, and 106 of female adult tick. Sixty-four of these OTUs had high similarity in the four developmental stages of H. flava. Eight bacterial genera had the highest abundances in all developmental stages, namely, Rickettsia, Coxiella, Pseudomonas, Ehrlichia, Escherichia, Acinetobacter, Citrobacter, and Cupriavidus. The nymph had the highest abundance of Coxiella, and the female adult tick had the highest abundance of Rickettsia. Staphylococcus and Wolbachia were detected in all developmental stages except the egg.
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, PR China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Duan
authors.affiliation: College of Veterinary Medicine, Hunan Agricultural University, Changsha, Hunan Province, PR China.
authors.forename: Tianyin
authors.initials: T
authors.lastname: Cheng
mesh_terms: D000150:Acinetobacter / Q000235:genetics / Q000302:isolation & purification; D000328:Adult; D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification; D003381:Coxiella / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005260:Female; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D026863:Ixodidae / Q000254:growth & development* / Q000382:microbiology*; D007814:Larva / Q000382:microbiology; D059013:Microbial Consortia / Q000235:genetics*; D009758:Nymph / Q000382:microbiology; D010063:Ovum / Q000382:microbiology; D010802:Phylogeny; D011549:Pseudomonas / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: Haemaphysalis flava; V3 region of the 16S rDNA; different developmental stages; illumina MiSeq; microbial community structure and diversity