title: Limited Link between Oxidative Stress and Ochratoxin A-Induced Renal Injury in an Acute Toxicity Rat Model.
abstract: Ochratoxin A (OTA) displays nephrotoxicity and hepatotoxicity. However, in the acute toxicity rat model, there is no evidence on the relationship between OTA and nephrotoxicity and hepatotoxicity. Based on this, the integrated analysis of physiological status, damage biomarkers, oxidative stress, and DNA damage were performed. After OTA treatment, the body weight decreased and AST, ALP, TP, and BUN levels in serum increased. Hydropic degeneration, swelling, vacuolization, and partial drop occurred in proximal tubule epithelial cells. PCNA and Kim-1 were dose-dependently increased in the kidney, but Cox-2 expression and proliferation were not found in the liver. In OTA-treated kidneys, the mRNA expressions ofKim-1,Cox-2,Lcn2, andCluwere dose-dependently increased. The mRNA expressions ofVimandCox-2were decreased in OTA-treated livers. Some oxidative stress indicators were altered in the kidneys (ROS and SOD) and livers (SOD and GSH). DNA damage and oxidative DNA damage were not found. In conclusion, there is a limited link between oxidative stress and OTA-induced renal injury in an acute toxicity rat model.
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing 100083, China. zlyhome@163.com.|The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China. zlyhome@163.com.
authors.forename: Liye
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing 100083, China. anhuiyutao@gmail.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Yu
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing 100083, China. qixiaozhe0706030224@126.com.
authors.forename: Xiaozhe
authors.initials: X
authors.lastname: Qi
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing 100083, China. gaojingsienna@gmail.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Gao
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing 100083, China. huangkl009@sina.com.|The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China. huangkl009@sina.com.|Beijing Laboratory of Food Quality and Safety, Beijing 100083, China. huangkl009@sina.com.
authors.forename: Kunlun
authors.initials: K
authors.lastname: Huang
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing 100083, China. raininghe@163.com.|The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China. raininghe@163.com.|Beijing Laboratory of Food Quality and Safety, Beijing 100083, China. raininghe@163.com.
authors.forename: Xiaoyun
authors.initials: X
authors.lastname: He
authors.affiliation: State key Laboratory of Agrobiotechnology, Department of Animal Physiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China. luohaoshu@cau.edu.cn.
authors.forename: Haoshu
authors.initials: H
authors.lastname: Luo
authors.affiliation: Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, No. 17 Tsinghua Donglu, Beijing 100083, China. xuwentao@cau.edu.cn.|The Supervision, Inspection and Testing Center of Genetically Modified Organisms, Ministry of Agriculture, Beijing 100083, China. xuwentao@cau.edu.cn.|Beijing Laboratory of Food Quality and Safety, Beijing 100083, China. xuwentao@cau.edu.cn.
authors.forename: Wentao
authors.initials: W
authors.lastname: Xu
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced* / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D000818:Animals; D001835:Body Weight / Q000187:drug effects; D001921:Brain / Q000187:drug effects / Q000473:pathology; D015815:Cell Adhesion Molecules / Q000235:genetics / Q000378:metabolism; D051152:Clusterin / Q000235:genetics; D051546:Cyclooxygenase 2 / Q000235:genetics; D004249:DNA Damage; D000071068:Lipocalin-2 / Q000235:genetics; D008099:Liver / Q000187:drug effects / Q000473:pathology; D008297:Male; D009793:Ochratoxins / Q000633:toxicity*; D009929:Organ Size / Q000187:drug effects; D018384:Oxidative Stress; D017208:Rats, Wistar; D013737:Testis / Q000187:drug effects / Q000473:pathology; D013950:Thymus Gland / Q000187:drug effects / Q000473:pathology; D023382:Toxicity Tests, Acute
chemical_list: D015815:Cell Adhesion Molecules; D051152:Clusterin; D051546:Cyclooxygenase 2; D000071068:Lipocalin-2; D009793:Ochratoxins; C498500:Clu protein, rat; C110936:Havcr1 protein, rat; C097278:Lcn2 protein, rat; C025589:ochratoxin A; C489691:Ptgs2 protein, rat
keywords: DNA damage; Ochratoxin A; acute toxicity; nephrotoxicity; oxidative stress