title: Evidences for Piperine inhibiting cancer by targeting human G-quadruplex DNA sequences.
abstract: Piperine, a naturally occurring alkaloid, is well known as anti-oxidant, anti-mutagenic, anti-tumor and anti-proliferative agent. Piperine exerts such pharmacological activities by binding or interacting with various cellular targets. Recently, the first report for Piperine interaction with duplex DNA has been published last year but its interaction with G-quadruplex structures has not been studied yet. Herein, we report for the first time the interaction of Piperine with various DNA G-quadruplex structures. Comprehensive biophysical techniques were employed to determine the basis of interaction for the complex formed between Piperine and G-quadruplex DNA sequences. Piperine showed specificity for G-quadruplex DNA over double stranded DNA, with highest affinity for G-quadruplex structure formed at c-myc promoter region. Further, in-vitro studies show that Piperine causes apoptosis-mediated cell death that further emphasizes the potential of this natural product, Piperine, as a promising candidate for targeting G-quadruplex structure and thus, acts as a potent anti-cancer agent.
authors.affiliation: Centre for Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore, Madhya Pradesh, 453552, India.
authors.forename: Arpita
authors.initials: A
authors.lastname: Tawani
authors.affiliation: Cellular and Molecular Neurobiology Unit, Indian Institute of Technology Jodhpur, Rajasthan, 342011, India.
authors.forename: Ayeman
authors.initials: A
authors.lastname: Amanullah
authors.affiliation: Cellular and Molecular Neurobiology Unit, Indian Institute of Technology Jodhpur, Rajasthan, 342011, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Mishra
authors.affiliation: Centre for Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, Indore, Madhya Pradesh, 453552, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
mesh_terms: D000072283:A549 Cells; D000470:Alkaloids / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D000970:Antineoplastic Agents / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D017209:Apoptosis / Q000187:drug effects; D052117:Benzodioxoles / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D001665:Binding Sites; D002942:Circular Dichroism; D024202:Electrophoretic Mobility Shift Assay; D054856:G-Quadruplexes / Q000187:drug effects*; D006801:Humans; D062105:Molecular Docking Simulation; D009369:Neoplasms / Q000188:drug therapy / Q000235:genetics / Q000473:pathology; D019906:Nuclear Magnetic Resonance, Biomolecular; D009690:Nucleic Acid Conformation; D009691:Nucleic Acid Denaturation; D010880:Piperidines / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D053284:Polyunsaturated Alkamides / Q000737:chemistry / Q000627:therapeutic use / Q000633:toxicity*; D011401:Promoter Regions, Genetic; D016271:Proto-Oncogene Proteins c-myc / Q000235:genetics; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D052117:Benzodioxoles; D010880:Piperidines; D053284:Polyunsaturated Alkamides; D016271:Proto-Oncogene Proteins c-myc; C008922:piperine