title: Virus-Enabled Biosensor for Human Serum Albumin.
abstract: The label-free detection of human serum albumin (HSA) in aqueous buffer is demonstrated using a simple, monolithic, two-electrode electrochemical biosensor. In this device, both millimeter-scale electrodes are coated with a thin layer of a composite containing M13 virus particles and the electronically conductive polymer poly(3,4-ethylenedioxy thiophene) or PEDOT. These virus particles, engineered to selectively bind HSA, serve as receptors in this biosensor. The resistance component of the electrical impedance, Zre, measured between these two electrodes provides electrical transduction of HSA binding to the virus-PEDOT film. The analysis of sample volumes as small as 50 μL is made possible using a microfluidic cell. Upon exposure to HSA, virus-PEDOT films show a prompt increase in Zrewithin 5 s and a stable Zresignal within 15 min. HSA concentrations in the range from 100 nM to 5 μM are detectable. Sensor-to-sensor reproducibility of the HSA measurement is characterized by a coefficient-of-variance (COV) ranging from 2% to 8% across this entire concentration range. In addition, virus-PEDOT sensors successfully detected HSA in synthetic urine solutions.
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697-2025, United States.
authors.forename: Alana F
authors.initials: AF
authors.lastname: Ogata
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697-2025, United States.
authors.forename: Joshua M
authors.initials: JM
authors.lastname: Edgar
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697-2025, United States.
authors.forename: Sudipta
authors.initials: S
authors.lastname: Majumdar
authors.affiliation: PhageTech Inc. , 5151 California Avenue, Suite 150, Irvine, California 92617, United States.
authors.forename: Jeffrey S
authors.initials: JS
authors.lastname: Briggs
authors.affiliation: PhageTech Inc. , 5151 California Avenue, Suite 150, Irvine, California 92617, United States.
authors.forename: Shae V
authors.initials: SV
authors.lastname: Patterson
authors.affiliation: Wainamics Inc. , 3135 Osgood Court, Fremont, California 94539, United States.
authors.forename: Ming X
authors.initials: MX
authors.lastname: Tan
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697-2025, United States.
authors.forename: Stephan T
authors.initials: ST
authors.lastname: Kudlacek
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697-2025, United States.
authors.forename: Christine A
authors.initials: CA
authors.lastname: Schneider
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697-2025, United States.|PhageTech Inc. , 5151 California Avenue, Suite 150, Irvine, California 92617, United States.
authors.forename: Gregory A
authors.initials: GA
authors.lastname: Weiss
authors.affiliation: Department of Chemistry, University of California, Irvine , Irvine, California 92697-2025, United States.|PhageTech Inc. , 5151 California Avenue, Suite 150, Irvine, California 92617, United States.
authors.forename: Reginald M
authors.identifier: 0000-0003-2831-3028
authors.initials: RM
authors.lastname: Penner
mesh_terms: D017104:Bacteriophage M13 / Q000737:chemistry*; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019086:Bridged Bicyclo Compounds, Heterocyclic / Q000737:chemistry*; D004553:Electric Conductivity; D017097:Electric Impedance; D004566:Electrodes; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D011108:Polymers / Q000737:chemistry*; D015203:Reproducibility of Results; D000075462:Serum Albumin, Human / Q000032:analysis / Q000652:urine*; D014771:Virion / Q000737:chemistry*
chemical_list: D019086:Bridged Bicyclo Compounds, Heterocyclic; D011108:Polymers; C121383:poly(3,4-ethylene dioxythiophene); D000075462:Serum Albumin, Human