title: Modification of the deoxyribose test to detect strong iron binding.
abstract: Deoxyribose test has been widely used for determination of reactivities of various compounds for the hydroxyl radical. The test is based on the formation of hydroxyl radical by Fe2+complex in the Fenton reaction. We propose a modification of the deoxyribose test to detect strong iron binding, inhibiting participation of Fe2+in the Fenton reaction, on the basis of examination of concentration dependence of deoxyribose degradation on Fe2+concentration, at a constant concentration of a chelating agent.
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Sadowska-Bartosz
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland.
authors.forename: Sabina
authors.initials: S
authors.lastname: Galiniak
authors.affiliation: Department of Biochemistry and Cell Biology, Faculty of Biology and Agriculture, University of Rzeszów, Rzeszów, Poland.|Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Łódź, Łódź, Poland.
authors.forename: Grzegorz
authors.initials: G
authors.lastname: Bartosz
mesh_terms: D001665:Binding Sites; D002614:Chelating Agents / Q000737:chemistry; D019411:Clinical Laboratory Techniques / Q000379:methods; D003855:Deoxyribose / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D006861:Hydrogen Peroxide / Q000737:chemistry*; D017665:Hydroxyl Radical / Q000737:chemistry*; D007501:Iron / Q000737:chemistry*; D007502:Iron Chelating Agents / Q000737:chemistry
chemical_list: D002614:Chelating Agents; C045076:Fenton's reagent; D007502:Iron Chelating Agents; D017665:Hydroxyl Radical; D003855:Deoxyribose; D006861:Hydrogen Peroxide; D007501:Iron
keywords: DETAPA; EDTA; Fenton reaction; chelation; deoxyribose test; desferrioxamine; hydrogen peroxide; hydroxyl radical; iron; superoxide