title: miR-106a suppresses tumor cells death in colorectal cancer through targeting ATG7.
abstract: Autophagy-related gene 7 (ATG7) and miR-106a play an important role in cancer cell autophagy and apoptosis, but the outcome of ATG7 and miR-106a in colorectal cancer (CRC) still remains not clear. In this study, we found that ATG7 and miR-106a expression were mutually related with cell death and prognosis in CRC patients. In addition, we also showed that ATG7 and miR-106a expression were changeable in colorectal cancer cell lines when compared with normal cell lines, but ATG7 and miR-106a mRNA level was negatively correlated. Furthermore, ATG7 protein and mRNA levels decreased after over-expression of miR-106a, whereas the suppression of ATG7 had the opposite effect. We confirmed that miR-106a down-regulated ATG7 mRNA level by binding the specific sequence of ATG7 mRNA 3'UTR region. Moreover, the over-expression of ATG7 induced CRC cells death both in vitro and in vivo. Taken together, our study data demonstrated that ATG7 aggravated the cell death of CRC, which was inhibited by miR-106a.
authors.affiliation: Department of General Surgery, Medical College of Nanchang University, Second Affiliated Hospital of Nanchang University, No. 461 Bayi Road, Nanchang, 330006, Jiangxi, China. haoweipei104@163.com.
authors.forename: Haibin
authors.initials: H
authors.lastname: Hao
authors.affiliation: Department of General Surgery, Medical College of Nanchang University, Second Affiliated Hospital of Nanchang University, No. 461 Bayi Road, Nanchang, 330006, Jiangxi, China.
authors.forename: Guangfeng
authors.initials: G
authors.lastname: Xia
authors.affiliation: Department of General Surgery, Medical College of Nanchang University, Second Affiliated Hospital of Nanchang University, No. 461 Bayi Road, Nanchang, 330006, Jiangxi, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Medical College of Nanchang University, Second Affiliated Hospital of Nanchang University, No. 461 Bayi Road, Nanchang, 330006, Jiangxi, China.
authors.forename: Fuping
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Department of General Surgery, Medical College of Nanchang University, Second Affiliated Hospital of Nanchang University, No. 461 Bayi Road, Nanchang, 330006, Jiangxi, China.
authors.forename: Laipeng
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of General Surgery, Medical College of Nanchang University, Second Affiliated Hospital of Nanchang University, No. 461 Bayi Road, Nanchang, 330006, Jiangxi, China.
authors.forename: Dong
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D000368:Aged; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D000071193:Autophagy-Related Protein 7 / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D002460:Cell Line; D045744:Cell Line, Tumor; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000601:surgery; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D008207:Lymphatic Metastasis; D008297:Male; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D011379:Prognosis; D016019:Survival Analysis
chemical_list: C542541:MIRN106 microRNA, human; D035683:MicroRNAs; C509349:ATG7 protein, human; D000071193:Autophagy-Related Protein 7
keywords: ATG7; Cell death; Colorectal cancer; miR-106a