title: Molecular evidence on the protective effect of ellagic acid on phosalone-induced senescence in rat embryonic fibroblast cells.
abstract: Salient evidence testifies the link between organophosphorus (OPs) exposure and the formation of free radical oxidants; and it is well accepted that free radicals are one of the basic concerns of senescence. To show the oxidative features of phosalone (PLN) as a key member of OPs, to induce senescence in rat embryonic fibroblast (REF) cells and to demonstrate the beneficial effects of the known antioxidant ellagic acid (EA) in diminishing the PLN-induced toxic effects, the levels of cell viability, oxidative stress markers, inflammatory cytokines, telomerase activity, and the expression of the genes related to senescence were investigated. Our results lend support to the hypothesis that PLN enhances the entire premature senescence parameters of REF cells. This accounts for the mechanistic approval of the role of OPs in induction of senescence in rat fibroblasts. Moreover, incorporation of EA diminished PLN toxicity mainly through suppression of p38 and p53 at gene and protein levels, and tempered the inflammation factors (TNF-α, IL-1β, IL-6 and NF-κB), which further affected cell division. Analysis of cell cycle showed that the percentage of G0/G1 arrest, in REF cells treated by EA was elevated as compared to control and PLN treated cells.
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Baeeri
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran; Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.
authors.forename: Saeideh
authors.initials: S
authors.lastname: Momtaz
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Navaei-Nigjeh
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran; International Campus, Tehran University of Medical Sciences (IC-TUMS), Tehran, Iran.
authors.forename: Kamal
authors.initials: K
authors.lastname: Niaz
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahban
authors.initials: M
authors.lastname: Rahimifard
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyedeh Farnaz
authors.initials: SF
authors.lastname: Ghasemi-Niri
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran; Department of Biology, Faculty of Sciences, University of Zabol, Zabol, Iran.
authors.forename: Nima
authors.initials: N
authors.lastname: Sanadgol
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahshid
authors.initials: M
authors.lastname: Hodjat
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Sharifzadeh
authors.affiliation: Toxicology and Diseases Group, Pharmaceutical Sciences Research Group, Tehran University of Medical Sciences, Tehran, Iran; International Campus, Tehran University of Medical Sciences (IC-TUMS), Tehran, Iran; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Tehran University of Medical Science, Tehran, Iran. Electronic address: Mohammad@TUMS.Ac.Ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Abdollahi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002453:Cell Cycle / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D016922:Cellular Senescence / Q000187:drug effects*; D004610:Ellagic Acid / Q000494:pharmacology*; D004622:Embryo, Mammalian / Q000187:drug effects* / Q000473:pathology; D005260:Female; D005347:Fibroblasts / Q000187:drug effects* / Q000473:pathology; D007306:Insecticides / Q000633:toxicity; D009946:Organothiophosphorus Compounds / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D011247:Pregnancy; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D007306:Insecticides; D009946:Organothiophosphorus Compounds; D004610:Ellagic Acid; C003117:phosalone
keywords: Aging; Cell cycle; Ellagic acid; Inflammatory cytokines; Oxidative stress; Phosalone; Senescence