title: Signal transduction mechanism for glucagon-inducedleptingene expression in goldfish liver.
abstract: Leptin is a peripheral satiety hormone that also plays important roles in energy homeostasis in vertebrates ranging from fish to mammals. In teleost fish, however, the regulatory mechanism for leptin gene expression still remains unclear. In this study, we found that glucagon, a key hormone in glucose homeostasis, was effective at elevating theleptin-AI andleptin-AII transcript levels in goldfish liver via bothin vivointraperitoneal injection andin vitrocells incubation approaches. The responses ofleptin-AI andleptin-AII mRNA to glucagon treatment were highly comparable. In contrast, blockade of local glucagon action could reduce the basal and inducedleptin-AI andleptin-AII mRNA expression. The stimulation of leptin levels by glucagon was caused by the activation of adenylate cyclase (AC)/cyclic-AMP (cAMP)/ protein kinase A (PKA), and probably cAMP response element-binding protein (CREB) cascades. Our study described the effect and signal transduction mechanism of glucagon on leptin gene expression in goldfish liver, and may also provide new insight into leptin as a mediator in the regulatory network of energy metabolism in the fish model.
authors.affiliation: School of stomatology and medicine, Foshan University, Foshan 528000, China.
authors.forename: Ai-Fen
authors.initials: AF
authors.lastname: Yan
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB); South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Chen
authors.affiliation: School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, University of Hong Kong, Hong Kong, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Chen
authors.affiliation: School of stomatology and medicine, Foshan University, Foshan 528000, China.
authors.forename: Dong-Sheng
authors.initials: DS
authors.lastname: Tang
authors.affiliation: School of stomatology and medicine, Foshan University, Foshan 528000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB).
authors.forename: Xiao
authors.initials: X
authors.lastname: Jiang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB).
authors.forename: Wen
authors.initials: W
authors.lastname: Huang
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB); South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China.
authors.forename: Chun-Hua
authors.initials: CH
authors.lastname: Ren
authors.affiliation: CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB); South China Sea Bio-Resource Exploitation and Utilization Collaborative Innovation Center, Guangzhou 510275, China.
authors.forename: Chao-Qun
authors.initials: CQ
authors.lastname: Hu
mesh_terms: D000262:Adenylyl Cyclases / Q000378:metabolism; D000818:Animals; D000242:Cyclic AMP / Q000378:metabolism; D017362:Cyclic AMP Response Element-Binding Protein / Q000235:genetics / Q000378:metabolism; D017868:Cyclic AMP-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D005934:Glucagon / Q000494:pharmacology*; D006054:Goldfish / Q000235:genetics / Q000378:metabolism*; D020738:Leptin / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D020738:Leptin; D005934:Glucagon; D000242:Cyclic AMP; D017868:Cyclic AMP-Dependent Protein Kinases; D000262:Adenylyl Cyclases
keywords: gene expression; glucagon; goldfish.; leptin; signal transduction