title: Multicenter evaluation of crystal violet decolorization assay (CVDA) for rapid detection of isoniazid and rifampicin resistance in Mycobacterium tuberculosis.
abstract: The aim of this multicenter study was to evaluate the performance of the crystal violet decolorization assay (CVDA) for detection of multidrug resistant tuberculosis (MDR-TB). This study was performed in 11 centers in two phases. A total of 156 isolates were tested for INH and RIF resistance. In the phase I, 106 clinical isolates were tested in the Center 1-7. In the phase 2, 156 clinical isolates were tested in the center 1-6, center 8-11. Eighty six of 156 tested isolates were the same in phase I. Agreements were 96.2-96.8% for INH and 98.1-98.7% for RIF in the phase I-II, respectively. Mean time to obtain the results in the phase I was 14.3 ± 5.4 days. In the phase II, mean time to obtain the results was 11.6 ± 3.5 days. Test results were obtained within 14days for 62.3% (66/106) of isolates in the phase I and 81.4% (127/156) of isolates in the phase II. In conclusion, CVDA is rapid, reliable, inexpensive, and easy to perform for rapid detection of MDR-TB isolates. In addition, it could be adapted for drug susceptibility testing with all drugs both in developed and developing countries.
authors.affiliation: Ondokuz Mayis University Medical School Department of Medical Microbiology, Samsun, Turkey.
authors.forename: Ahmet Yilmaz
authors.initials: AY
authors.lastname: Coban
authors.affiliation: Ondokuz Mayis University Medical School Department of Medical Microbiology, Samsun, Turkey.
authors.forename: Ahmet Ugur
authors.initials: AU
authors.lastname: Akbal
authors.affiliation: Dr. Suat Seren Chest Diseases and Chest Surgery Training and Research Hospital, Medical Microbiology Laboratory, Izmir, Turkey.
authors.forename: Can
authors.initials: C
authors.lastname: Bicmen
authors.affiliation: Gulhane Military Medical Academy, Department of Medical Microbiology, Ankara, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Albay
authors.affiliation: Gulhane Military Medical Academy, Department of Medical Microbiology, Ankara, Turkey.
authors.forename: Ali Korhan
authors.initials: AK
authors.lastname: Sig
authors.affiliation: Istanbul University Istanbul Medical School Department of Medical Microbiology, Istanbul, Turkey.
authors.forename: Meltem
authors.initials: M
authors.lastname: Uzun
authors.affiliation: Istanbul University Istanbul Medical School Department of Medical Microbiology, Istanbul, Turkey.
authors.forename: Deniz Sertel
authors.initials: DS
authors.lastname: Selale
authors.affiliation: Celal Bayar University Medical School Department of Medical Microbiology, Manisa, Turkey.
authors.forename: Nuri
authors.initials: N
authors.lastname: Ozkutuk
authors.affiliation: Celal Bayar University Medical School Department of Medical Microbiology, Manisa, Turkey.
authors.forename: Suheyla
authors.initials: S
authors.lastname: Surucuoglu
authors.affiliation: Public Health Agency of Turkey Tuberculosis Reference Laboratory, Ankara, Turkey.
authors.forename: Nurhan
authors.initials: N
authors.lastname: Albayrak
authors.affiliation: Public Health Agency of Turkey Tuberculosis Reference Laboratory, Ankara, Turkey.
authors.forename: Nilay
authors.initials: N
authors.lastname: Ucarman
authors.affiliation: Dokuz Eylul University Medical School Department of Medical Microbiology, Izmir, Turkey.
authors.forename: Aydan
authors.initials: A
authors.lastname: Ozkutuk
authors.affiliation: Dokuz Eylul University Medical School Department of Medical Microbiology, Izmir, Turkey.
authors.forename: Nuran
authors.initials: N
authors.lastname: Esen
authors.affiliation: Atatürk Chest Diseases and Chest Surgery Training and Research Hospital Medical Microbiology Laboratory, Ankara, Turkey.
authors.forename: Ismail
authors.initials: I
authors.lastname: Ceyhan
authors.affiliation: Haydarpasa Military Medical Academy, Department of Medical Microbiology, Istanbul, Turkey.
authors.forename: Mustafa
authors.initials: M
authors.lastname: Ozyurt
authors.affiliation: Haydarpasa Military Medical Academy, Department of Medical Microbiology, Istanbul, Turkey.
authors.forename: Bayhan
authors.initials: B
authors.lastname: Bektore
authors.affiliation: Mersin University Medical School Department of Medical Microbiology, Mersin, Turkey.
authors.forename: Gonul
authors.initials: G
authors.lastname: Aslan
authors.affiliation: Mersin University Medical School Department of Medical Microbiology, Mersin, Turkey.
authors.forename: Nuran
authors.initials: N
authors.lastname: Delialioğlu
authors.affiliation: Hacettepe University Medical School Department of Medical Microbiology, Ankara, Turkey.
authors.forename: Alpaslan
authors.initials: A
authors.lastname: Alp
mesh_terms: D002151:Calorimetry; D019049:Developed Countries; D005840:Gentian Violet / Q000494:pharmacology*; D006801:Humans; D007538:Isoniazid / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests; D009169:Mycobacterium tuberculosis / Q000187:drug effects / Q000302:isolation & purification*; D012293:Rifampin / Q000494:pharmacology*; D012680:Sensitivity and Specificity; D013997:Time Factors; D018088:Tuberculosis, Multidrug-Resistant / Q000175:diagnosis*
chemical_list: D005840:Gentian Violet; D007538:Isoniazid; D012293:Rifampin