title: Antibody-coupled hydroxyapatite nanoparticles as efficient tools for labeling intracellular proteins.
abstract: Smart biomaterials for active targeting are a novel way for biosensing, gene and drug delivery, and bioimaging. The functional additives are chosen according to the material carrier characteristics, i.e. the functional mercapto acids of different lengths. In order to identify the target tissue, cell, organ or molecule, the biomaterial must be equipped with a recognizing molecule on its surface. In most cases, semiconductor o metal materials are employed in bioimaging and biosensing applications; in gene and drug delivery area, it is useful to employ porous nanoparticles as carriers. Hydroxyapatite nanoparticles have been proved efficiently in drug delivery. In this work we established a new protocol to obtain smart hydroxyapatite nanoparticles with 3-mercaptopropionic acid and anti-Actin molecules in order to localize actin molecules in cells.
authors.affiliation: Programa de Doctorado de Nanociencias y Nanotecnología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN), Av. IPN 2508, Col. San Pedro Zacatenco, C.P. 07360 Ciudad de México, Mexico. Electronic address: fvazquez_84@yahoo.com.mx.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Vázquez-Hernández
authors.affiliation: Centro de Investigación en Computación del Instituto Politécnico Nacional (CIC-IPN), Av. Juan de Dios Bátiz, Esq. Miguel Othón de Mendizábal, Col. Nueva Industrial Vallejo, C.P. 07738 Ciudad de México, Mexico. Electronic address: smendozaa10@gmail.com.
authors.forename: Salvador
authors.initials: S
authors.lastname: Mendoza-Acevedo
authors.affiliation: Laboratorio de Nanobiotecnología, Centro de Investigación en Micro y Nanotecnología (MICRONA), Universidad Veracruzana, C.P. 94294 Boca del Río, Veracruz, Mexico. Electronic address: cmendoza_barrera@hotmail.com.
authors.forename: Claudia Oliva
authors.initials: CO
authors.lastname: Mendoza-Barrera
authors.affiliation: Departamento de Física, Cinvestav-IPN, Av. IPN 2508, Col. San Pedro Zacatenco, C.P. 07360 Ciudad de México, Mexico. Electronic address: juliogma1@gmail.com.
authors.forename: Julio
authors.initials: J
authors.lastname: Mendoza-Álvarez
authors.affiliation: Programa de Doctorado de Nanociencias y Nanotecnología, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav-IPN), Av. IPN 2508, Col. San Pedro Zacatenco, C.P. 07360 Ciudad de México, Mexico; Departamento de Biología Celular, Cinvestav-IPN, Av. IPN 2508, Col. San Pedro Zacatenco, C.P. 07360 Ciudad de México, Mexico. Electronic address: jpluna2003@gmail.com.
authors.forename: Juan Pedro
authors.initials: JP
authors.lastname: Luna-Arias
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D045744:Cell Line, Tumor; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D017886:Durapatite / Q000737:chemistry*; D005260:Female; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*
chemical_list: D000906:Antibodies; D020099:Coated Materials, Biocompatible; D017886:Durapatite
keywords: Bioconjugation; Cell viability; Functionalization; Hydroxyapatite; Immunofluorescence; Nanoparticle