title: Neoalbaconol inhibits angiogenesis and tumor growth by suppressing EGFR-mediated VEGF production.
abstract: Neoalbaconol, derived from Albatrellus confluens, shows anti-cancer activities in the previously study, but its role in angiogenesis is unknown. Here, we determined whether neoalbaconol could attenuate angiogenesis and how does it occur. Data demonstrated that neoalbaconol could inhibit the proliferation of breast cancer cells and induce apoptosis. Also, neoalbaconol suppressed vascular endothelial growth factor (VEGF)-induced human umbilical vascular endothelial cells (HUVECs) proliferation, migration, invasion, and capillary-like tube formation in vitro and reduced tumor angiogenesis in vivo. VEGF receptor activation and the downstream signal transduction cascades activation were inhibited by neoalbaconol. Additionally, neoalbaconol blocked EGFR-mediated VEGF production. EGFR overexpression reversed the neoalbaconol-induced VEGF reduction, confirming the importance of the EGFR inhibition in anti-angiogenesis of neoalbaconol. Furthermore, neoalbaconol inhibited tumor growth and tumor angiogenesis in a breast cancer xenograft model in vivo. Taken together, these results indicate that neoalbaconol could inhibit tumor angiogenesis and growth through direct suppression effects on vascular endothelial cells and reduction of proangiogenic factors in cancer cells.
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.|Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Xinfang
authors.initials: X
authors.lastname: Yu
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.|Department of radiology, The Third Xiangya Hospital of Central South University, Hunan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Qipan
authors.initials: Q
authors.lastname: Deng
authors.affiliation: The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: You
authors.affiliation: The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Haidan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Songling
authors.initials: S
authors.lastname: Peng
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Xiaolan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Jingchen
authors.initials: J
authors.lastname: Lu
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Xiangjian
authors.initials: X
authors.lastname: Luo
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Yang
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Min
authors.initials: M
authors.lastname: Tang
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Xinxian
authors.initials: X
authors.lastname: Weng
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yi
authors.affiliation: Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Liu
authors.affiliation: Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Shengqi
authors.initials: S
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Yunnan, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Ding
authors.affiliation: School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Feng
authors.affiliation: Key Laboratory of Carcinogenesis of Chinese Ministry of Public Health, Liver Cancer Institute, Zhongshan Hospital, Shanghai, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Carcinogenesis of Chinese Ministry of Public Health, Liver Cancer Institute, Zhongshan Hospital, Shanghai, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Fan
authors.affiliation: The Hormel Institute, University of Minnesota, Austin, Minnesota.
authors.forename: Ann M
authors.initials: AM
authors.lastname: Bode
authors.affiliation: The Hormel Institute, University of Minnesota, Austin, Minnesota.
authors.forename: Zigang
authors.initials: Z
authors.lastname: Dong
authors.affiliation: School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.
authors.forename: Jikai
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Cancer Carcinogenesis and Invasion of Chinese Ministry of Education, Xiangya Hospital, Central South University, Hunan, China.|Cancer Research Institute, Xiangya School of Medicine, Central South University, Hunan, China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Cao
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000098:blood supply / Q000188:drug therapy* / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D066246:ErbB Receptors / Q000235:genetics / Q000378:metabolism*; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects; D006801:Humans; D008819:Mice, Nude; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000378:metabolism; D012717:Sesquiterpenes / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*; D023041:Xenograft Model Antitumor Assays
chemical_list: D000970:Antineoplastic Agents; D012717:Sesquiterpenes; C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A; C586893:neoalbaconol; C512478:EGFR protein, human; D066246:ErbB Receptors
keywords: EGFR; VEGF; angiogenesis; breast cancer; neoalbaconol