title: Fluorescent pH-Sensing Probe Based on Biorefinery Wood Lignosulfonate and Its Application in Human Cancer Cell Bioimaging.
abstract: A water-soluble, ratiometric fluorescent pH probe, L-SRhB, was synthesized via grafting spirolactam Rhodamine B (SRhB) to lignosulfonate (LS). As the ring-opening product of L-SRhB, FL-SRhB was also prepared. The pH-response experiment indicated that L-SRhB showed a rapid response to pH changes from 4.60 to 6.20 with a pKaof 5.35, which indicated that L-SRhB has the potential for pH detection of acidic organelle. In addition, the two probes were internalized successfully by living cells through the endocytosis pathway and could distinguish normal cells from cancer cells by different cell staining rates. In addition, L-SRhB showed obvious cytotoxicity to cancer cells, whereas it was nontoxic to normal cells in the same condition. L-SRhB might have potential in cancer therapy. L-SRhB might be a promising ratiometric fluorescent pH sensor and bioimaging dye for the recognition of cancer cells. The results also provided a new perspective to the high-value utilization of lignin.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou, China.|State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou, China.
authors.forename: Yuyuan
authors.initials: Y
authors.lastname: Xue
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou, China.|State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou, China.
authors.forename: Wanshan
authors.initials: W
authors.lastname: Liang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou, China.|State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou, China.|State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou, China.
authors.forename: Xinwen
authors.identifier: http://orcid.org/0000-0002-4575-256X
authors.initials: X
authors.lastname: Peng
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou, China.|State Key Laboratory of Pulp and Paper Engineering, South China University of Technology , Guangzhou, China.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology , Guangzhou, China.
authors.forename: Jinbin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute of Biomass Chemistry and Utilization, Beijing Forestry University , Beijing, China.
authors.forename: Runcang
authors.identifier: http://orcid.org/0000-0003-2721-6357
authors.initials: R
authors.lastname: Sun
mesh_terms: D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008031:Lignin / Q000031:analogs & derivatives* / Q000737:chemistry; D009369:Neoplasms / Q000175:diagnosis*; D012235:Rhodamines / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D012235:Rhodamines; C001545:lignosulfuric acid; D008031:Lignin; C029773:rhodamine B
keywords: FRET; Rhodamine B; biomass; cancer sensing; ratiometric sensor