title: QCM-based aptamer selection and detection of Salmonella typhimurium.
abstract: In this study, quartz crystal microbalance (QCM) was used to select aptamers against Salmonella typhimurium. To increase the success rate of Systematic Evolution of Ligands Exponential Enrichment (SELEX), the affinity of DNA pool in each round was simultaneously tracked using QCM in order to avoid the loss of high-quality aptamers. When the frequency change reached a maximum value after several rounds of selection and counter-selection, the candidate pool was cloned and sequenced. Out of three aptamer candidates, aptamer B5 showed high specificity and binding affinity with dissociation constant (Kdvalue) of 58.5nM, and was chosen for further studies. Subsequently, a QCM-based aptasensor was developed to detect S. typhimurium. This aptasensor was able to detect 103CFU/mL of S. typhimurium with less than 1h. This study demonstrated QCM-based selection could be more effective selection of aptamers and QCM-based aptasensor could be more sensitive in detecting S. typhimurium.
authors.affiliation: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Department of Biological & Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Lijun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Biological & Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Ronghui
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Agronomy, Shenyang Agricultural University, Shenyang 110866, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Tieshan
authors.initials: T
authors.lastname: Jiang
authors.affiliation: Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Hong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA.
authors.forename: Michael
authors.initials: M
authors.lastname: Slavik
authors.affiliation: Jiangxi-OAI Joint Research Institute, Nanchang University, Nanchang 330047, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wei
authors.affiliation: Department of Biological & Agricultural Engineering, University of Arkansas, Fayetteville, AR 72701, USA; Center of Excellence for Poultry Science, University of Arkansas, Fayetteville, AR 72701, USA. Electronic address: yanbinli@uark.edu.
authors.forename: Yanbin
authors.initials: Y
authors.lastname: Li
mesh_terms: D058525:Quartz Crystal Microbalance Techniques; D052156:SELEX Aptamer Technique; D012486:Salmonella typhimurium / Q000302:isolation & purification*
keywords: Aptamer; Detection; Quartz crystal microbalance; SELEX; Salmonella typhimurium