title: Evaluation of AhR-agonists and AhR-agonist activity in sediments of Liaohe River protected areas, China.
abstract: A total of 9 sediment samples of Liaohe River protected areas were collected to evaluate aryl hydrocarbon receptor agonists (AhR-agonists) and AhR-agonist activity via chemical analysis and in vitro H4IIE cell bioassay. Results indicated that bioassay-derived 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (Bio-TEQs) ranged from 89.1 to 251.1pg/g dry weight. Concentrations of 16 EPA polycyclic aromatic hydrocarbons (PAHs), 12 dioxin-like polychlorinated biphenyls (PCBs), and polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) ranged from 256.8 to 560.1ng/g, 79.2 to 416.2pg/g, and 199.6 to 538.4pg/g, respectively. According to potency balance analysis, TEQchems based on PAHs, PCBs, and PCDD/Fs could contribute 16.56% to 26.11% of Bio-TEQs. This could be explained by the potential existence of unidentified AhR-agonists and the potential non-additive interactions among AhR-agonists in sediment extracts. Through the different contributions to Bio-TEQs, this study confirms that PCDD/Fs were the main pollutants that induced significantly AhR-agonist activity in sediments of Liaohe River protected areas.
authors.affiliation: Northeast Key Laboratory of Arable Land Conservation and Improvement, Ministry of Agriculture, National Engineering Laboratory for Efficient Utilization of Soil and Fertilizer Resources, College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, PR China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, PR China. Electronic address: kex@iae.ac.cn.
authors.forename: Xin
authors.initials: X
authors.lastname: Ke
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, PR China.
authors.forename: Shaofeng
authors.initials: S
authors.lastname: Gui
authors.affiliation: Department of Pharmacy, Land force General Hospital of PLA, Beijing 100000, PR China.
authors.forename: Xiaoqiong
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, PR China.
authors.forename: Chunyong
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Energy and Environment, Shenyang Aerospace University, Shenyang 110136, PR China.
authors.forename: Haijun
authors.initials: H
authors.lastname: Zhang
mesh_terms: D001572:Benzofurans / Q000032:analysis; D002681:China; D019015:Geologic Sediments / Q000737:chemistry*; D011078:Polychlorinated Biphenyls / Q000032:analysis; D000072317:Polychlorinated Dibenzodioxins / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D018336:Receptors, Aryl Hydrocarbon / Q000819:agonists*; D045483:Rivers; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D001572:Benzofurans; D000072317:Polychlorinated Dibenzodioxins; D011084:Polycyclic Aromatic Hydrocarbons; D018336:Receptors, Aryl Hydrocarbon; D014874:Water Pollutants, Chemical; D011078:Polychlorinated Biphenyls
keywords: AhR-agonist activity; Aryl hydrocarbon receptor agonists (AhR-agonists); In vitro H4IIE cell bioassay; Sediments