title: Induction of a robust immunity response against novel duck reovirus in ducklings using a subunit vaccine of sigma C protein.
abstract: Novel duck reovirus (NDRV) disease emerged in China in 2011 and continues to cause high morbidity and about 5.0 to 50% mortality in ducklings. Currently there are no approved vaccines for the virus. This study aimed to assess the efficacy of a new vaccine created from the baculovirus and sigma C gene against NDRV. In this study, a recombinant baculovirus containing the sigma C gene was constructed, and the purified protein was used as a vaccine candidate in ducklings. The efficacy of sigma C vaccine was estimated according to humoral immune responses, cellular immune response and protection against NDRV challenge. The results showed that sigma C was highly expressed in Sf9 cells. Robust humoral and cellular immune responses were induced in all ducklings immunized with the recombinant sigma C protein. Moreover, 100% protection against lethal challenge with NDRV TH11 strain was observed. Summary, the recombinant sigma C protein could be utilized as a good candidate against NDRV infection.
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.|College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Zhuangli
authors.initials: Z
authors.lastname: Bi
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.|College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Yingqi
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Zongyan
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Chuanfeng
authors.initials: C
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
authors.forename: Guijun
authors.initials: G
authors.lastname: Wang
authors.affiliation: Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, China.
authors.forename: Guangqing
authors.initials: G
authors.lastname: Liu
mesh_terms: D000818:Animals; D004372:Ducks; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D000071497:Immunogenicity, Vaccine; D030101:Orthoreovirus, Avian / Q000276:immunology*; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control*; D011994:Recombinant Proteins / Q000276:immunology; D012088:Reoviridae Infections / Q000276:immunology / Q000517:prevention & control*; D061987:Sf9 Cells; D022223:Vaccines, Subunit / Q000276:immunology; D014764:Viral Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D011994:Recombinant Proteins; D022223:Vaccines, Subunit; D014764:Viral Proteins