title: Functional Analysis of the Fusarielin Biosynthetic Gene Cluster.
abstract: Fusarielins are polyketides with a decalin core produced by various species ofAspergillusandFusarium. Although the responsible gene cluster has been identified, the biosynthetic pathway remains to be elucidated. In the present study, members of the gene cluster were deleted individually in aFusarium graminearumstrain overexpressing the local transcription factor. The results suggest that atrans-acting enoyl reductase (FSL5) assists the polyketide synthase FSL1 in biosynthesis of a polyketide product, which is released by hydrolysis by atrans-acting thioesterase (FSL2). Deletion of the epimerase (FSL3) resulted in accumulation of an unstable compound, which could be the released product. A novel compound, named prefusarielin, accumulated in the deletion mutant of the cytochrome P450 monooxygenase FSL4. Unlike the known fusarielins fromFusarium, this compound does not contain oxygenized decalin rings, suggesting that FSL4 is responsible for the oxygenation.
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. aidadroce@gmail.com.
authors.forename: Aida
authors.initials: A
authors.lastname: Droce
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. wagmasaei@gmail.com.
authors.forename: Wagma
authors.initials: W
authors.lastname: Saei
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. sijoer89@gmail.com.
authors.forename: Simon Hartung
authors.initials: SH
authors.lastname: Jørgensen
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. rw@bio.aau.dk.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wimmer
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. hgiese@bio.aau.dk.
authors.forename: Henriette
authors.initials: H
authors.lastname: Giese
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. rwo@bio.aau.dk.
authors.forename: Rasmus Dam
authors.initials: RD
authors.lastname: Wollenberg
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. tes@bio.aau.dk.
authors.forename: Teis Esben
authors.initials: TE
authors.lastname: Sondergaard
authors.affiliation: Department of Chemistry and Bioscience, Aalborg University, Fredrik Bajers Vej 7H, DK-9220 Aalborg Ø, Denmark. jls@bio.aau.dk.|Department of Chemistry and Bioscience, Aalborg University, Niels Bohrs Vej 8, 6700 Esbjerg, Denmark. jls@bio.aau.dk.
authors.forename: Jens Laurids
authors.initials: JL
authors.lastname: Sørensen
mesh_terms: D001230:Aspergillus / Q000235:genetics; D005670:Fusarium / Q000235:genetics*; D005800:Genes, Fungal; D015394:Molecular Structure; D005810:Multigene Family; D061065:Polyketides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D061065:Polyketides
keywords: Fusarium graminearum; PKS; biosynthetic pathway; decalin; overexpression; polyketide synthases; secondary metabolites; trans-enoyl reductase; transcription factor