title: The effects of di 2-ethyl hexyl phthalate (DEHP) on cellular lipid accumulation in HepG2 cells and its potential mechanisms in the molecular level.
abstract: Diethylhexyl phthalate (DEHP) is suspected to be an inevitable factor related to metabolic disease. Our previous study demonstrated that excess DEHP could exacerbate non-alcoholic fatty liver disease (NAFLD) in SD rats. Addressing the terra incognita in DEHP-induced metabolic dysfunction, this study used HepG2 cells to investigate the potential mechanisms involved in DEHP-induced toxicity in vitro. The cells were established lipid overload model with oleic acid and BSA, then exposed to different concentrations (5, 10, 25, 50, 100 μmol/l DEHP) of DEHP for further analysis. The Oil Red O staining results showed that DEHP could promote lipid accumulation in cells. The level of superoxide dismutase (SOD) and malondialdehyde (MDA) changed suggested the balance of oxidative stress was disrupted. Additionally, western blot analysis showed that DEHP could promote the expression of peroxisome proliferator-activated receptor α (PPARα) and sterol regulatory element-binding protein 1c (SREBP-1c). By quantifying the expressions of the two proteins, it is of interest to determine that DEHP could promote lipid accumulation in hepatocytes via activating the SREBP-1c and PPARα-signaling pathway.
authors.affiliation: a Key Laboratory of Anti-inflammatory and Immune Medicine of Education Ministry, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine , Institute of Clinical Pharmacology of Anhui Medical University , Hefei , Anhui , China.
authors.forename: Wang
authors.initials: W
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Anti-inflammatory and Immune Medicine of Education Ministry, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine , Institute of Clinical Pharmacology of Anhui Medical University , Hefei , Anhui , China.
authors.forename: Xin-Yue
authors.initials: XY
authors.lastname: Shen
authors.affiliation: a Key Laboratory of Anti-inflammatory and Immune Medicine of Education Ministry, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine , Institute of Clinical Pharmacology of Anhui Medical University , Hefei , Anhui , China.
authors.forename: Wen-Wen
authors.initials: WW
authors.lastname: Zhang
authors.affiliation: a Key Laboratory of Anti-inflammatory and Immune Medicine of Education Ministry, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine , Institute of Clinical Pharmacology of Anhui Medical University , Hefei , Anhui , China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: b Affiliated Anhui Provincial Hospital, Anhui Medical University , Hefei , Anhui , China.
authors.forename: Wei-Ping
authors.initials: WP
authors.lastname: Xu
authors.affiliation: a Key Laboratory of Anti-inflammatory and Immune Medicine of Education Ministry, Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine , Institute of Clinical Pharmacology of Anhui Medical University , Hefei , Anhui , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
mesh_terms: D018929:Cell Culture Techniques; D004051:Diethylhexyl Phthalate / Q000633:toxicity*; D004305:Dose-Response Relationship, Drug; D056945:Hep G2 Cells; D006801:Humans; D050356:Lipid Metabolism / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D018613:Microscopy, Confocal; D019301:Oleic Acid / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D047493:PPAR alpha / Q000378:metabolism; D010968:Plasticizers / Q000633:toxicity*; D051780:Sterol Regulatory Element Binding Protein 1 / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D047493:PPAR alpha; D010968:Plasticizers; D051780:Sterol Regulatory Element Binding Protein 1; D019301:Oleic Acid; D008315:Malondialdehyde; D004051:Diethylhexyl Phthalate; D013482:Superoxide Dismutase
keywords: DEHP; PPAR; ROS; SREBP; lipid accumulation