title: Calmodulin Regulated Spectrin Associated Protein 1 mRNA is Directly Regulated by miR-126 in Primary Human Osteoblasts.
abstract: Vascularization is essential for bone development, fracture healing, and bone tissue engineering. We have previously described that coculture of primary human osteoblasts (hOBs) and human umbilical vein endothelial cells (HUVECs) improves differentiation of both cell types. Investigating the role of microRNAs (miRNAs) in this system, we found that miR-126 is highly upregulated in hOBs following coculturing with HUVECs. In this study we performed miR-126 gain-of-function and loss-of-function experiments in hOBs followed by microarray analysis in order to identify targets of miR-126. The transcript cluster IDs were sieved by applying cut-off criteria and by selecting transcripts which were upregulated following miR-126 downregulation and vice versa. The calmodulin regulated spectrin associated protein 1 (CAMSAP1) mRNA was confirmed to be differentially regulated by miR-126. Using the luciferase reporter assay it was demonstrated that CAMSAP1 is directly targeted by miR-126. In this study, we show that miR-126 and CAMSAP1 directly interact in hOBs. This finding has potential implications for tissue engineering applications. J. Cell. Biochem. 118: 1756-1763, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany.
authors.forename: Sandra
authors.initials: S
authors.lastname: Strassburg
authors.affiliation: Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany.
authors.forename: Nikita
authors.initials: N
authors.lastname: Nabar
authors.affiliation: Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany.
authors.forename: Florian
authors.initials: F
authors.lastname: Lampert
authors.affiliation: Department of Radiology, Ortenau Klinikum Offenburg-Gengenbach, Offenburg, Germany.
authors.forename: Sebastian M
authors.initials: SM
authors.lastname: Goerke
authors.affiliation: Department of Hematology and Oncology, Freiburg University Medical Center, Freiburg, 79106, Germany.
authors.forename: Dietmar
authors.initials: D
authors.lastname: Pfeifer
authors.affiliation: Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany.
authors.forename: Günter
authors.initials: G
authors.lastname: Finkenzeller
authors.affiliation: Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany.
authors.forename: Gerhard B
authors.initials: GB
authors.lastname: Stark
authors.affiliation: Departments of Plastic and Hand Surgery, University of Freiburg Medical Center, Freiburg, 79106, Germany.
authors.forename: Filip
authors.initials: F
authors.lastname: Simunovic
mesh_terms: D016723:Bone Remodeling / Q000235:genetics / Q000502:physiology; D003599:Cytoskeleton / Q000378:metabolism; D005109:Extracellular Matrix / Q000378:metabolism; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism*; D008869:Microtubule-Associated Proteins / Q000235:genetics* / Q000378:metabolism*; D010006:Osteoblasts / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D023822:Tissue Engineering
chemical_list: C000588920:CAMSAP1 protein, human; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; D008869:Microtubule-Associated Proteins; D012333:RNA, Messenger
keywords: CALMODULIN REGULATED SPECTRIN ASSOCIATED PROTEIN 1; PRIMARY HUMAN OSTEOBLASTS; REGULATION OF GENE EXPRESSION; miR-126