title: Terminal-Specific Interaction between Double-Stranded DNA Layers: Colloidal Dispersion Behavior and Surface Force.
abstract: Double-stranded DNA-grafted nanoparticles (dsDNA-NPs) exhibit a unique dispersion behavior under high-salt conditions depending on the pairing status of their outermost base pairs (pairing or unpairing). The dsDNA-NPs having complementary (i.e., pairing) outermost base pairs spontaneously aggregate under high-salt conditions, but not when their outermost base pairs are mismatched (unpairing). In this study, we used colloidal probe atomic force microscopy to examine how the outermost base pairs affect the interaction between the dsDNA-grafted layers (dsDNA layers). To precisely assess the subtle structural differences in the dsDNA layers, we developed a method for the formation of a homogenous dsDNA layer on gold surfaces using hairpin-shaped DNAs. Homogenous dsDNA layers having complementary (G-C) or mismatched (C-C) outermost base pairs were grafted onto the surfaces of colloidal probes and gold substrates. Force-distance curves measured in an aqueous medium under high-salt conditions revealed that the surface forces between the dsDNA layers were bilateral in nature and were governed by the outermost base pairs. Between complementary outermost dsDNA layers, the surface force changed from repulsive to attractive with an increase in the NaCl concentration (10-1000 mM). The attraction observed under high-salt conditions was attributed to the site-specific interaction proceeded only between complementary dsDNA terminals, the so-called blunt-end stacking. In fact, between mismatched outermost dsDNA layers, the repulsive force was mostly dominant within the same NaCl concentration range. Our results clearly revealed that the pairing status of the outermost base pairs has significant implications for the surface forces between dsDNA layers, leading to the unique dispersion behavior of dsDNA-NPs.
authors.affiliation: Interdisciplinary Graduate School of Science and Technology, Shinshu University , 4-7-1 Wakasato, Nagano, Nagano 380-8553, Japan.|Bioengineering Laboratory, RIKEN , 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Naoki
authors.initials: N
authors.lastname: Kanayama
authors.affiliation: Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology , 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Taito
authors.initials: T
authors.lastname: Sekine
authors.affiliation: Bioengineering Laboratory, RIKEN , 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Ozasa
authors.affiliation: Bioengineering Laboratory, RIKEN , 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Kishi
authors.affiliation: Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology , 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nyu
authors.affiliation: Department of Materials Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology , 4259 Nagatsuta-cho, Midori-ku, Yokohama, Kanagawa 226-8502, Japan.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Hayashi
authors.affiliation: Bioengineering Laboratory, RIKEN , 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
authors.forename: Mizuo
authors.initials: M
authors.lastname: Maeda
mesh_terms: D020029:Base Pairing; D003102:Colloids / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006046:Gold; D018625:Microscopy, Atomic Force
chemical_list: D003102:Colloids; D006046:Gold; D004247:DNA