title: Feasibility study: protein denaturation and coagulation monitoring with speckle variance optical coherence tomography.
abstract: We performed the feasibility study using speckle variance optical coherence tomography (SvOCT) to monitor the thermally induced protein denaturation and coagulation process as a function of temperature and depth. SvOCT provided the depth-resolved image of protein denaturation and coagulation with microscale resolution. This study was conducted using egg white. During the heating process, as the temperature increased, increases in the speckle variance signal was observed as the egg white proteins coagulated. Additionally, by calculating the cross-correlation coefficient in specific areas, denaturized egg white conditions were successfully estimated. These results indicate that SvOCT could be used to monitor the denaturation process of various proteins.
authors.affiliation: Johns Hopkins University, Department of Electrical and Computer Engineering, 3400 North Charles Street, Baltimore, Maryland 21218, United States.
authors.forename: Changho
authors.initials: C
authors.lastname: Lee
authors.affiliation: Johns Hopkins University, Department of Electrical and Computer Engineering, 3400 North Charles Street, Baltimore, Maryland 21218, United States.
authors.forename: Gyeongwoo
authors.initials: G
authors.lastname: Cheon
authors.affiliation: U.S. Food and Drug Administration, Center for Devices and Radiological Health, 10903 New Hampshire Avenue, Silver Spring, Maryland 20993, United States.
authors.forename: Do-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Johns Hopkins University, Department of Electrical and Computer Engineering, 3400 North Charles Street, Baltimore, Maryland 21218, United States.
authors.forename: Jin U
authors.initials: JU
authors.lastname: Kang
mesh_terms: D000818:Animals; D004529:Egg White / Q000032:analysis; D005240:Feasibility Studies; D008991:Monitoring, Physiologic / Q000295:instrumentation* / Q000379:methods*; D066329:Protein Aggregates; D011489:Protein Denaturation; D041623:Tomography, Optical Coherence
chemical_list: D066329:Protein Aggregates