title: Molecular Characterization of AnimalFasciolaspp. Isolates from Kermanshah, Western Iran.
abstract: BACKGROUND
  We evaluated the genetic diversity of samples identified morphologically asFasciolaspp. from sheep, cattle and goat from Kermanshah Province, western Iran using PCR-RFLP method.
  
  
  METHODS
  We used PCR-RFLP analysis of ribosomal ITS1 fragment using RsaI restriction enzyme to investigate the genetic characteristics ofFasciolaspecies obtained from different hosts (16 sheep, 28 cattle, 4 goats). The species ofFasciolawere confirmed by sequencing the 700 bp region of ribosomal ITS1 gene.
  
  
  RESULTS
  In Kermanshah,F. hepaticawas present in 96% of the samples,F. giganticawas found only in two cattle sample. No hybrid forms were detected in the present study.
  
  
  CONCLUSIONS
  Our results contribute to clarify the dark spots ofFasciolagenotyping in different parts of Iran.
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Arezoo
authors.initials: A
authors.lastname: Bozorgomid
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran.
authors.forename: Naser
authors.initials: N
authors.lastname: Nazari
authors.affiliation: Dept. of Molecular Medicine, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Hamzeh
authors.initials: H
authors.lastname: Rahimi
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Eshrat
authors.initials: E
authors.lastname: Beigom Kia
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Homa
authors.initials: H
authors.lastname: Hajjaran
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Mohebali
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mojgan
authors.initials: M
authors.lastname: Aryaeipour
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Peyman
authors.initials: P
authors.lastname: Heidarian
authors.affiliation: Dept. of Food Hygiene, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Mohammad Saeid
authors.initials: MS
authors.lastname: Ezati
authors.affiliation: Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Center for Research of Endemic Parasites of Iran (CREPI), Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mohamad Bagher
authors.initials: MB
authors.lastname: Rokni
keywords: Fasciola gigantica; Fasciola hepatica; Genotype; Iran; PCR