title: Trp-Asp (WD) Repeat Domain 1 Is Essential for Mouse Peri-implantation Development and Regulates Cofilin Phosphorylation.
abstract: Trp-Asp (WD) repeat domain 1 (WDR1) is a highly conserved actin-binding protein across all eukaryotes and is involved in numerous actin-based processes by accelerating Cofilin severing actin filament. However, the function and the mechanism of WDR1 in mammalian early development are still largely unclear. We now report that WDR1 is essential for mouse peri-implantation development and regulates Cofilin phosphorylation in mouse cells. The disruption of maternal WDR1 does not obviously affect ovulation and female fertility. However, depletion of zygotic WDR1 results in embryonic lethality at the peri-implantation stage. In WDR1 knock-out cells, we found that WDR1 regulates Cofilin phosphorylation. Interestingly, WDR1 is overdosed to regulate Cofilin phosphorylation in mouse cells. Furthermore, we showed that WDR1 interacts with Lim domain kinase 1 (LIMK1), a well known phosphorylation kinase of Cofilin. Altogether, our results provide new insights into the role and mechanism of WDR1 in physiological conditions.
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing.|the Institute of Zoology, University of Chinese Academy of Sciences, Beijing 100049.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing.|the Institute of Zoology, University of Chinese Academy of Sciences, Beijing 100049.
authors.forename: Haixia
authors.initials: H
authors.lastname: Ma
authors.affiliation: the State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Nanjing University, Nanjing 210061, and.
authors.forename: Ping
authors.initials: P
authors.lastname: Wan
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing.|the Institute of Zoology, University of Chinese Academy of Sciences, Beijing 100049.
authors.forename: Dandan
authors.initials: D
authors.lastname: Qin
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing.|the Institute of Zoology, University of Chinese Academy of Sciences, Beijing 100049.
authors.forename: Xiaoxiao
authors.initials: X
authors.lastname: Wang
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing.
authors.forename: Xiaoxin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing.
authors.forename: Yunlong
authors.initials: Y
authors.lastname: Xiang
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing.
authors.forename: Wenbo
authors.initials: W
authors.lastname: Liu
authors.affiliation: the State Key Laboratory of Pharmaceutical Biotechnology and MOE Key Laboratory of Model Animals for Disease Study, Model Animal Research Center, Nanjing University, Nanjing 210061, and chenjiong@nju.edu.cn.
authors.forename: Jiong
authors.initials: J
authors.lastname: Chen
authors.affiliation: the Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Science and Engineering, Beijing University of Agriculture, Beijing 102206, China yizhaoh2009@163.com.
authors.forename: Zhaohong
authors.initials: Z
authors.lastname: Yi
authors.affiliation: From the State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 100101 Beijing, lil@ioz.ac.cn.|the Institute of Zoology, University of Chinese Academy of Sciences, Beijing 100049.
authors.forename: Lei
authors.identifier: 0000-0001-5478-5681
authors.initials: L
authors.lastname: Li
mesh_terms: D051339:Actin Depolymerizing Factors / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D010064:Embryo Implantation; D020964:Embryo Loss / Q000235:genetics / Q000378:metabolism; D004622:Embryo, Mammalian / Q000196:embryology*; D047108:Embryonic Development; D005260:Female; D054461:Lim Kinases / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D018345:Mice, Knockout; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D051339:Actin Depolymerizing Factors; D008840:Microfilament Proteins; C524014:Wdr1 protein, mouse; D054461:Lim Kinases; C515893:Limk1 protein, mouse
keywords: Limk1; WDR1; actin; cofilin; development; embryonic development; mouse genetics; phosphorylation