title: Determination of salicylic acid using a magnetic iron oxide nanoparticle-based solid-phase extraction procedure followed by an online concentration technique through micellar electrokinetic capillary chromatography.
abstract: In this study, a magnetic iron oxide nanoparticle-based solid-phase extraction procedure combined with the online concentration and separation of salicylic acid (SA) through micellar electrokinetic chromatography-UV detection (MEKC-UV) was developed. Under optimal experimental conditions, a good linearity in the range of 0.01-100μmolL-1was obtained with a coefficient of correlation of 0.9999. The detection sensitivity of the proposed method exhibited an approximately 1026-fold improvement compared with a single MEKC method without online concentration, and the detection limit (S/N=3) was 3.80nmolL-1. The repeatability of the method was evaluated using intraday and interday RSDs (11.5% and 17.0%, respectively). The method was used to determine SA concentrations in tobacco leaves (Nicotiana tabacum L. cv. Samsun) from the NN genotype, nn genotype, and Nt-NahG mutant strains, as well as in shampoo and ointment samples. Rapid extraction and separation (<50min), acceptable repeatability (RSD<17.0%), and high spiked recoveries (95.8%-102.4%) were observed for plants, detergents, and pharmaceuticals.
authors.affiliation: Department of Chemistry, National Changhua University of Education, Changhua City, Taiwan.
authors.forename: Yu-Hsuan
authors.initials: YH
authors.lastname: Chang
authors.affiliation: Department of Chemistry, National Changhua University of Education, Changhua City, Taiwan.
authors.forename: Chang-Wei
authors.initials: CW
authors.lastname: Huang
authors.affiliation: Department of Biology, National Changhua University of Education, Changhua, Taiwan.
authors.forename: Shih-Feng
authors.initials: SF
authors.lastname: Fu
authors.affiliation: Research Centre for Traditional Chinese Medicine, Department of Medical Research, China Medical University Hospital, Taichung City, Taiwan.
authors.forename: Mei-Yao
authors.initials: MY
authors.lastname: Wu
authors.affiliation: Department of Chemistry, University of Wisconsin-Platteville, Platteville, USA.
authors.forename: Tsunghsueh
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Chemistry, National Changhua University of Education, Changhua City, Taiwan. Electronic address: linywjerry@cc.ncue.edu.tw.
authors.forename: Yang-Wei
authors.initials: YW
authors.lastname: Lin
mesh_terms: D002623:Chemistry Techniques, Analytical / Q000295:instrumentation / Q000379:methods*; D020374:Chromatography, Micellar Electrokinetic Capillary; D005290:Ferric Compounds / Q000737:chemistry*; D057230:Limit of Detection; D008823:Micelles; D053758:Nanoparticles / Q000737:chemistry*; D018515:Plant Leaves / Q000737:chemistry*; D020156:Salicylic Acid / Q000032:analysis*; D052616:Solid Phase Extraction; D014026:Nicotiana / Q000737:chemistry
chemical_list: D005290:Ferric Compounds; D008823:Micelles; C000499:ferric oxide; D020156:Salicylic Acid
keywords: Magnetic iron oxide nanoparticles; Micellar electrokinetic chromatography; Online concentration; Salicylic acid; Solid-phase extraction