title: Investigation of Cellular Uptakes of the In-Tether Chiral-Center-Induced Helical Pentapeptides.
abstract: We recently reported that a precisely positioned in-tether chiral center can modulate backbone peptides' secondary structures, which provides an unbiased platform to evaluate peptides' biophysical properties solely imposed by secondary structure differences. In this work, we studied the cellular uptake efficiency and mechanism of epimer pairs of a panel of chirality-induced helical peptides (CIH peptides). Although the peptides' cellular uptake is a synergetic result of various factors, our results unambiguously indicate that helical content is an important factor for the cellular uptake of CIH peptides.
authors.affiliation: Department of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University , Shenzhen, Guangdong 518055, China.
authors.forename: Kuan
authors.identifier: http://orcid.org/0000-0003-2448-2254
authors.initials: K
authors.lastname: Hu
authors.affiliation: Department of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University , Shenzhen, Guangdong 518055, China.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University , Shenzhen, Guangdong 518055, China.
authors.forename: Mengying
authors.initials: M
authors.lastname: Yu
authors.affiliation: Department of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University , Shenzhen, Guangdong 518055, China.
authors.forename: Chengjie
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Chemical Biology and Biotechnology, Shenzhen Graduate School of Peking University , Shenzhen, Guangdong 518055, China.
authors.forename: Zigang
authors.initials: Z
authors.lastname: Li
mesh_terms: D002942:Circular Dichroism; D005434:Flow Cytometry; D057809:HEK293 Cells; D006801:Humans; D010455:Peptides / Q000737:chemistry* / Q000493:pharmacokinetics*; D017433:Protein Structure, Secondary; D013237:Stereoisomerism
chemical_list: D010455:Peptides