title: Gene expression and physiological responses associated to stomatal functioning in Rosa×hybrida grown at high relative air humidity.
abstract: High relative air humidity (RH≥85%) during growth disturbs stomatal functioning, resulting in excessive water loss in conditions of high evaporative demand. We investigated the expression of nine abscisic acid (ABA)-related genes (involved in ABA biosynthesis, oxidation and conjugation) and two non-ABA related genes (involved in the water stress response) aiming to better understand the mechanisms underlying contrasting stomatal functioning in plants grown at high RH. Four rose genotypes with contrasting sensitivity to high RH (one sensitive, one tolerant and two intermediate) were grown at moderate (62±3%) or high (89±4%) RH. The sensitive genotype grown at high RH showed a significantly higher stomatal conductance (gs) and water loss in response to closing stimuli as compared to the other genotypes. Moreover, high RH reduced the leaf ABA concentration and its metabolites to a greater extent in the sensitive genotype as compared to the tolerant one. The large majority of the studied genes had a relevant role on stomatal functioning (NCED1, UGT75B2, BG2, OST1, ABF3 and Rh-APX) while two others showed a minor contribution (CYP707A3 and BG1) and AAO3, CYP707A1 and DREB1B did not contribute to the tolerance trait. These results show that multiple genes form a highly complex regulatory network acting together towards the genotypic tolerance to high RH.
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal. Electronic address: dalia.alvescarvalho@wur.nl.
authors.forename: Dália R A
authors.initials: DR
authors.lastname: Carvalho
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal.
authors.forename: Marta W
authors.initials: MW
authors.lastname: Vasconcelos
authors.affiliation: Plant Sciences Group, Wageningen University, Plant Physiology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands; Gyeongsangbuk-Do Agricultural Research & Extension Services, 136 Gil-14, Chilgokiungang-Daero, Daegu, South Korea.
authors.forename: Sangseok
authors.initials: S
authors.lastname: Lee
authors.affiliation: Plant Sciences Group, Wageningen University, Plant Breeding, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Carole F S
authors.initials: CF
authors.lastname: Koning-Boucoiran
authors.affiliation: Plant Sciences Group, Wageningen University, Plant Physiology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Dick
authors.initials: D
authors.lastname: Vreugdenhil
authors.affiliation: Plant Sciences Group, Wageningen University, Plant Breeding, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Frans A
authors.initials: FA
authors.lastname: Krens
authors.affiliation: Plant Sciences Group, Wageningen University, Horticulture and Product Physiology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands.
authors.forename: Ep
authors.initials: E
authors.lastname: Heuvelink
authors.affiliation: CBQF - Centro de Biotecnologia e Química Fina - Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, Apartado 2511, 4202-401 Porto, Portugal; Plant Sciences Group, Wageningen University, Horticulture and Product Physiology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands; GreenUP/CITAB-UP & DGAOT, Faculty of Sciences, University of Porto, Campus Agrário de Vairão, Rua Padre Armando Quintas, 7. 4485-661 Vairão, Portugal. Electronic address: susana.carvalho@fc.up.pt.
authors.forename: Susana M P
authors.initials: SM
authors.lastname: Carvalho
mesh_terms: D000040:Abscisic Acid / Q000096:biosynthesis*; D018506:Gene Expression Regulation, Plant; D006813:Humidity; D054046:Plant Stomata / Q000502:physiology*; D027826:Rosa / Q000502:physiology*
chemical_list: D000040:Abscisic Acid
keywords: Abscisic acid; Stomatal anatomy; Tetraploid roses; Vapor pressure deficit; Water loss