title: Carryover of aflatoxins from feed to lambari fish (Astyanax altiparanae) tissues.
abstract: The aim of this study was to verify the carryover of aflatoxin B1from feed to lambari fish. Aflatoxins (AF) were incorporated into feed, checking the levels by HPLC. Treatments were: Control, feed without toxin; A, feed + 10 µg AFB1kg-1; B, feed + 20 µg AFB1kg-1; and C, feed + 50 µg AFB1kg-1. Juveniles of lambari fish were placed in 12 aquariums at a density of 50 fish/m2. Fish were fed twice a day with extruded feed, at 5% of animal biomass. The unit sample was constituted by a pool of 10 fish. AFs B1, B2, G1, G2and M1were quantified by HPLC in fish muscle and liver after 30, 60, 90 and 120 days of experiment. There was accumulation of AFs is fish liver and muscle, mainly after 90 days. Fish from treatment C had higher levels of AFB1in muscle when compared with the others, and AFB1in muscle at 120 days was similar to the levels in feed. Therefore, when lambari fish is exposed on a daily and long-term basis to AFs in feed, the regulation limits for AFs in animal feed do not guarantee safety for consumers.
authors.affiliation: a Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering , University of Sao Paulo - USP , Pirassununga , Brazil.
authors.forename: E C
authors.initials: EC
authors.lastname: Michelin
authors.affiliation: a Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering , University of Sao Paulo - USP , Pirassununga , Brazil.
authors.forename: M M
authors.initials: MM
authors.lastname: Massocco
authors.affiliation: a Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering , University of Sao Paulo - USP , Pirassununga , Brazil.
authors.forename: S H S
authors.initials: SH
authors.lastname: Godoy
authors.affiliation: a Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering , University of Sao Paulo - USP , Pirassununga , Brazil.
authors.forename: J C
authors.initials: JC
authors.lastname: Baldin
authors.affiliation: b Laboratory of Fish Biotechnology , Chico Mendes Institute for Biodiversity Conservation - ICMBio , Pirassununga , Brazil.
authors.forename: G S
authors.initials: GS
authors.lastname: Yasui
authors.affiliation: c Department of Basic Sciences, Faculty of Animal Science and Food Engineering , University of Sao Paulo - USP , Pirassununga , Brazil.
authors.forename: C G
authors.initials: CG
authors.lastname: Lima
authors.affiliation: d Veterinary Medical Diagnostic Laboratory, College of Veterinary Medicine , University of Missouri , Columbia , MO , USA.
authors.forename: G E
authors.initials: GE
authors.lastname: Rottinghaus
authors.affiliation: a Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering , University of Sao Paulo - USP , Pirassununga , Brazil.
authors.forename: R L M
authors.initials: RL
authors.lastname: Sousa
authors.affiliation: a Department of Veterinary Medicine, Faculty of Animal Science and Food Engineering , University of Sao Paulo - USP , Pirassununga , Brazil.
authors.forename: A M
authors.initials: AM
authors.lastname: Fernandes
mesh_terms: D000348:Aflatoxins / Q000032:analysis* / Q000493:pharmacokinetics; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D060126:Characidae; D002851:Chromatography, High Pressure Liquid; D005506:Food Contamination / Q000032:analysis*; D008099:Liver / Q000737:chemistry*; D009132:Muscles / Q000737:chemistry*; D014018:Tissue Distribution
chemical_list: D000348:Aflatoxins
keywords: AFB1; Mycotoxins; aquiculture; food safety; liver; muscle; residues