title: Integrated analysis reveals candidate mRNA and their potential roles in uterine leiomyomas.
abstract: OBJECTIVE
  Uterine leiomyomas (UL) are the most common pelvic tumors, and the etiology and pathophysiology are not well understood. We aimed to elucidate the genes responsible for UL development.
  
  
  METHODS
  Integrated analyses of four datasets of mRNA profiling for UL were performed. Functional annotation of differentially expressed genes (DEG) was used to systematically characterize the global expression profiles. The UL-specific protein-protein interaction network was constructed.
  
  
  RESULTS
  Integrated analysis led to the discovery of 2167 DEG (1042 upregulated and 1125 downregulated). The aberrant expression of NAV2, KIF5C, DCX, CAPN6, COL4A2, ALDH1A1, and DPT may play important roles in UL tumorigenesis. In addition, the dysregulation of MEST, LGALS3, and TLR3 may be involved in the progression of UL by a common mechanism. Functional annotation showed that extracellular matrix receptor interaction may be more active and cause the extracellular matrix abnormally formed in UL. Moreover, focal adhesion and cell adhesion molecules may play roles in the development of UL. Furthermore, chemokine signaling pathway and cytokine-cytokine receptor interaction were most probably involved in the development of UL.
  
  
  CONCLUSIONS
  In conclusion, our study observed that a set of aberrantly expressed genes and the related biochemical pathways may lead to transformation of normal myometrium in pathological focuses.
authors.affiliation: Department of Ultrasonography, TaiShan Medical College Affiliated Hospital of Shandong Province, Taian, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Xia
authors.affiliation: Department of Ultrasonography, TaiShan Medical College Affiliated Hospital of Shandong Province, Taian, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Personnel Section, TaiShan Medical College Affiliated Hospital of Shandong Province, Taian, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Pathology, TaiShan Medical College Affiliated Hospital of Shandong Province, Taian, China.
authors.forename: Shengyi
authors.initials: S
authors.lastname: Dongye
authors.affiliation: Department of Ultrasonography, TaiShan Medical College Affiliated Hospital of Shandong Province, Taian, China.
authors.forename: Dewei
authors.initials: D
authors.lastname: Wang
mesh_terms: D016208:Databases, Factual; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D020022:Genetic Predisposition to Disease; D006801:Humans; D007889:Leiomyoma / Q000235:genetics*; D040081:Protein Array Analysis; D060066:Protein Interaction Maps; D012333:RNA, Messenger / Q000235:genetics*; D015854:Up-Regulation; D014594:Uterine Neoplasms / Q000235:genetics*
chemical_list: D012333:RNA, Messenger
keywords: differentially expressed genes; integrated analysis; pathways; uterine leiomyomas