title: Autophagy Protects from Raddeanin A-Induced Apoptosis in SGC-7901 Human Gastric Cancer Cells.
abstract: Raddeanin A (RA) is an extractive fromAnemone raddeana Regel, a traditional Chinese medicine. The aim of this study is to assess the efficacy of RA against human gastric cancer (GC) cells (SGC-7901) and explore its mechanism. MTT assay showed that RA inhibition of proliferation of SGC-7901 cells increased in a dose-dependent manner. Flow cytometry analysis and Hoechst 33258 staining showed that RA induced apoptosis on SGC-7901 cells. Meanwhile, it induced autophagy. Western blotting analysis showed that the RA induces apoptosis and autophagy by activating p38 MAPK pathway and inhibiting mTOR pathway. Further studies showed that autophagy inhibition could protect from RA-induced apoptosis in SGC-7901 cells. In conclusion, RA can induce SGC-7901 cell apoptosis and autophagy by activating p38 MAPK pathway. And autophagy can protect SGC-7901 cells from apoptosis induced by RA.
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Yu-Hao
authors.identifier: 0000-0002-1475-1599
authors.initials: YH
authors.lastname: Teng
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Jie-Pin
authors.identifier: 0000-0002-3060-3625
authors.initials: JP
authors.lastname: Li
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Shen-Lin
authors.initials: SL
authors.lastname: Liu
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Xi
authors.identifier: 0000-0003-2893-3526
authors.initials: X
authors.lastname: Zou
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Liang-Hua
authors.initials: LH
authors.lastname: Fang
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Jin-Yong
authors.initials: JY
authors.lastname: Zhou
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Wu
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Song-Yang
authors.initials: SY
authors.lastname: Xi
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China; Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China.
authors.forename: Song
authors.initials: S
authors.lastname: Xu
authors.affiliation: Oncologic Department, Jiangsu Province Hospital of TCM, Nanjing, Jiangsu 210029, China.
authors.forename: Rui-Ping
authors.identifier: 0000-0001-8065-4028
authors.initials: RP
authors.lastname: Wang