title: Evaluation of a commercial loop-mediated isothermal amplification assay for diagnosis of Bordetella pertussis infection.
abstract: We evaluated a commercial loop-mediated isothermal amplification (LAMP) assay kit for Bordetella pertussis detection. The LAMP primers were designed to target the ptxP1 allele of the pertussis toxin promoter, but the assay could detect B. pertussis ptxP3 and ptxP8 strains in addition to ptxP1 strains, with high analytical sensitivity.
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan. Electronic address: kamachi@nih.go.jp.
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kamachi
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan; Department of Pediatrics, St Marianna University School of Medicine, 2-16-1 Miyamae, Kawasaki 216-8511, Japan.
authors.forename: Takumi
authors.initials: T
authors.lastname: Moriuchi
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Hiramatsu
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: Nao
authors.initials: N
authors.lastname: Otsuka
authors.affiliation: Department of Bacteriology II, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayama, Tokyo 208-0011, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Shibayama
mesh_terms: D000483:Alleles; D001885:Bordetella Infections / Q000175:diagnosis* / Q000382:microbiology; D001886:Bordetella pertussis / Q000235:genetics / Q000302:isolation & purification*; D017931:DNA Primers; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005798:Genes, Bacterial; D021141:Nucleic Acid Amplification Techniques; D037342:Pertussis Toxin / Q000032:analysis; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D011933:Reagent Kits, Diagnostic; D012680:Sensitivity and Specificity
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial; D011933:Reagent Kits, Diagnostic; D037342:Pertussis Toxin
keywords: Bordetella pertussis; Loop-meditated isothermal amplification (LAMP); Pertussis toxin promoter; ptxP1; ptxP3