title: Effect of rosmarinic acid and carnosic acid on AGEs formation in vitro.
abstract: This work aimed to investigate the effect of the two main components of rosemary extracts, namely rosmarinic acid (RA) and carnosic acid (CA), on the formation of advanced glycation end-products (AGEs) in vitro. In the bovine serum albumin (BSA)/glucose model, addition of RA and CA at 400μg/mL inhibited fluorescent AGEs by more than 90%, and carboxymethyl lysine (CML) and carboxyethyl lysine (CEL) by 82.7% and 75.2%, and 71.4% and 64.2%, respectively. Moreover, the addition of RA and CA at 400μg/mL inhibited fluorescent AGEs by more than 90% both in the BSA/glyoxal (GO) and BSA/methylglyoxal (MGO) models, the formation of CML by 64.9% and 53.9% in BSA/GO model, and CEL by 28.9% and 24.3% in BSA/MGO model, respectively. RA and CA also significantly decreased the concentration of MGO and protein carbonylation.
authors.affiliation: Food and Nutritional Science Program, School of Biological Sciences, The University of Hong Kong, Hong Kong, China.
authors.forename: Juanying
authors.initials: J
authors.lastname: Ou
authors.affiliation: Institute of Integrated Chinese and Western Medicine, Medical College of Jinan University, Guangzhou 510632, China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Huang
authors.affiliation: Food and Nutritional Science Program, School of Biological Sciences, The University of Hong Kong, Hong Kong, China. Electronic address: mfwang@hku.hk.
authors.forename: Mingfu
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China. Electronic address: tosy@jnu.edu.cn.
authors.forename: Shiyi
authors.initials: S
authors.lastname: Ou
mesh_terms: D045784:Abietanes / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology*; D000818:Animals; D002417:Cattle; D002934:Cinnamates / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology*; D053630:Depsides / Q000032:analysis / Q000378:metabolism* / Q000494:pharmacology*; D005947:Glucose / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000037:antagonists & inhibitors* / Q000378:metabolism*; D006037:Glyoxal / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D050050:Protein Carbonylation / Q000187:drug effects / Q000502:physiology; D011765:Pyruvaldehyde / Q000378:metabolism; D012710:Serum Albumin, Bovine / Q000378:metabolism; D000097123:Rosmarinic Acid
chemical_list: D045784:Abietanes; D002934:Cinnamates; D053630:Depsides; D005947:Glucose; D017127:Glycation End Products, Advanced; D006037:Glyoxal; D008239:Lysine; D011765:Pyruvaldehyde; D012710:Serum Albumin, Bovine; D000097123:Rosmarinic Acid; C018381:salvin
keywords: Advanced glycation end-products; Carboxyethyl lysine; Carboxymethyl lysine; Dicarbonyl compounds; Rosemary extracts