title: Incorporation of biguanide compounds into poly(GL)-b-poly(GL-co-TMC-co-CL)-b-poly(GL) monofilament surgical sutures.
abstract: A new biodegradable coating was developed for bioabsorbable monofilament sutures. Specifically, a random copolymer having 35wt-% and 65wt-% of lactide and trimethylene carbonate units showed appropriate flexibility, stickiness and degradation rate, as well as capability to produce a complete and uniform coating. Monofilament sutures of polyglycolide-b-poly(glycolide-co-trimethylene carbonate-co-ε-caprolactone)-b-polyglycolide were loaded with chlorhexidine (CHX) and poly(hexamethylene biguanide) (PHMB) to explore the possibility to achieve antimicrobial activity without adverse cytotoxic effects. To this end, two processes based on single drug adsorption onto the suture surface and incorporation into the coating copolymer were used and subsequently evaluated. Although the second process could be considered more complex, clear benefits were observed in terms of drug loading efficiency, antimicrobial effect and even lack of cytotoxicity. In general, drugs could be loaded in an amount leading to a clear bacteriostatic effect for both Gram-negative and Gram-positive bacteria without causing significant cytotoxicity. Release profiles of PHMB and CHX were clearly different. Specifically, adsorption of the drug onto the fiber surface which prevented complete release was detected for PHMB. This polymer had advantages derived from its high molecular size, which hindered penetration into cells, thus resulting in lower cytotoxicity. Furthermore, bacterial growth kinetics measurements and bacterial adhesion assays showed greater effectiveness of this polymer.
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Av. Diagonal 647, Barcelona E-08028, Spain; B. Braun Surgical S.A., Carretera de Terrasa 121, Rubí, Barcelona, 08191, Spain.
authors.forename: Yolanda
authors.initials: Y
authors.lastname: Márquez
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Av. Diagonal 647, Barcelona E-08028, Spain.
authors.forename: Tània
authors.initials: T
authors.lastname: Cabral
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Av. Diagonal 647, Barcelona E-08028, Spain.
authors.forename: Alice
authors.initials: A
authors.lastname: Lorenzetti
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Av. Diagonal 647, Barcelona E-08028, Spain.
authors.forename: Lourdes
authors.initials: L
authors.lastname: Franco
authors.affiliation: B. Braun Surgical S.A., Carretera de Terrasa 121, Rubí, Barcelona, 08191, Spain.
authors.forename: Pau
authors.initials: P
authors.lastname: Turon
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Av. Diagonal 647, Barcelona E-08028, Spain.
authors.forename: Luís J
authors.initials: LJ
authors.lastname: Del Valle
authors.affiliation: Departament d'Enginyeria Química, Universitat Politècnica de Catalunya, Av. Diagonal 647, Barcelona E-08028, Spain. Electronic address: Jordi.Puiggali@upc.edu.
authors.forename: Jordi
authors.initials: J
authors.lastname: Puiggalí
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D001645:Biguanides / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D019556:COS Cells; D002710:Chlorhexidine / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D002522:Chlorocebus aethiops; D004926:Escherichia coli / Q000254:growth & development*; D011100:Polyglycolic Acid / Q000737:chemistry / Q000493:pharmacokinetics / Q000494:pharmacology; D013212:Staphylococcus epidermidis / Q000254:growth & development*; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D001645:Biguanides; D011100:Polyglycolic Acid; D002710:Chlorhexidine
keywords: Antibacterial agent; Chlorhexidine; Cytotoxicity; Drug release; Monofilament sutures; Poly(hexamethylene biguanide); Polymer coating