title: The pathogen-inducible promoter of defense-related LsGRP1 gene from Lilium functioning in phylogenetically distinct species of plants.
abstract: A suitable promoter greatly enhances the efficiency of target gene expression of plant molecular breeding and farming; however, only very few promoters are available for economically important non-graminaceous ornamental monocots. In this study, an 868-bp upstream region of defense-related LsGRP1 of Lilium, named PLsGRP1, was cloned by genome walking and proven to exhibit promoter activity in Nicotiana benthamiana and Lilium 'Stargazer' as assayed by agroinfiltration-based β-glucuronidase (GUS) expression system. Many putative biotic stress-, abiotic stress- and physiological regulation-related cis-acting elements were found in PLsGRP1. Serial deletion analysis of PLsGRP1performed in Nicotiana tabacum var. Wisconsin 38 accompanied with types of treatments indicated that 868-bp PLsGRP1was highly induced upon pathogen challenges and cold stress while the 131-bp 3'-end region of PLsGRP1could be dramatically induced by many kinds of abiotic stresses, biotic stresses and phytohormone treatments. Besides, transient GUS expression in a fern, gymnosperms, monocots and dicots revealed good promotor activity of PLsGRP1in many phylogenetically distinct plant species. Thus, pathogen-inducible PLsGRP1and its 131-bp 3'-end region are presumed potential as tools for plant molecular breeding and farming.
authors.affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan, ROC. Electronic address: d95633001@ntu.edu.tw.
authors.forename: Chia-Hua
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Plant Pathology and Microbiology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 10617, Taiwan, ROC. Electronic address: cychen@ntu.edu.tw.
authors.forename: Chao-Ying
authors.initials: CY
authors.lastname: Chen
mesh_terms: D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant; D005818:Genetic Engineering / Q000379:methods; D018745:Genome, Plant; D005966:Glucuronidase / Q000032:analysis; D027762:Lilium / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D011401:Promoter Regions, Genetic; D014026:Nicotiana / Q000235:genetics*
chemical_list: D010940:Plant Proteins; D005966:Glucuronidase
keywords: Agroinfiltration; Inducible promoter; LsGRP1; Non-graminaceous monocot