title: Cationic Amphiphilic Polymers with Antimicrobial Activity for Oral Care Applications: Eradication of S. mutans Biofilm.
abstract: The antibacterial and antibiofilm activities of cationic amphiphilic methacrylate polymers against cariogenic bacterium S. mutans were investigated. Cationic homopolymer PE0and copolymer PE31containing 31 mol % of ethyl methacrylate were synthesized by reversible addition-fragmentation chain transfer polymerization. These polymers displayed bactericidal activity toward S. mutans and prevented biofilm formation by killing the planktonic bacteria. At a concentration of 1000 μg/mL when incubated for 2 h the polymers reduced >80% of biofilm biomass. When the polymer assay solution with the biofilm was vigorously mixed using a pipet for 30 s, >50% of biofilm mass was removed at a polymer concentration of 250 μg/mL. Chlorhexidine and a cationic surfactant failed to reduce the biofilm mass at the same concentration. PE0was the most effective in removing biofilm and did not show any significant cytotoxicity to human gingival fibroblast and periodontal ligament stem cells when incubated for 10 min.
authors.affiliation: Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Haruko
authors.initials: H
authors.lastname: Takahashi
authors.affiliation: Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Enrico T
authors.initials: ET
authors.lastname: Nadres
authors.affiliation: Department of Biologic and Materials Sciences, School of Dentistry, University of Michigan , Ann Arbor, Michigan 48109, United States.
authors.forename: Kenichi
authors.identifier: 0000-0002-2935-8351
authors.initials: K
authors.lastname: Kuroda
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects* / Q000254:growth & development; D002412:Cations / Q000737:chemistry*; D006801:Humans; D008826:Microbial Sensitivity Tests; D009910:Oral Hygiene / Q000379:methods*; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology*; D013295:Streptococcus mutans / Q000187:drug effects* / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D002412:Cations; D011108:Polymers