title: Magnetically Oriented Bicelles with Monoalkylphosphocholines: Versatile Membrane Mimetics for Nuclear Magnetic Resonance Applications.
abstract: Bicelles (bilayered micelles) are model membranes used in the study of peptide structure and membrane interactions. They are traditionally made of long- and short-chain phospholipids, usually dimyristoylphosphatidylcholine (D14PC) and dihexanoyl-PC (D6PC). They are attractive membrane mimetics because their composition and planar surface are similar to the native membrane environment. In this work, to improve the solubilization of membrane proteins and allow their study in bicellar systems, D6PC was replaced by detergents from the monoalkylphosphocholine (MAPCHO) family, of which dodecylphosphocholine (12PC) is known for its ability to solubilize membrane proteins. More specifically 12PC, tetradecyl- (14PC), and hexadecyl-PC (16PC) have been employed. To verify the possibility of making bicelles with different hydrophobic thicknesses to better accommodate membrane proteins, D14PC was also replaced by phospholipids with different alkyl chain lengths: dilauroyl-PC (D12PC), dipalmitoyl-PC (D16PC), distearoyl-PC (D18PC), and diarachidoyl-PC (D20PC). Results obtained by31P solid-state nuclear magnetic resonance (NMR) and isothermal titration calorimetry (ITC) at several lipid-to-detergent molar ratios (q) and temperatures indicate that these new MAPCHO bicelles can be formed under a variety of conditions. The quality of their alignment is similar to that of classical bicelles, and the low critical micelle concentration (CMC) of the surfactants and their miscibility with phospholipids are likely to be advantageous for the reconstitution of membrane proteins.
authors.affiliation: Département de Chimie, Université du Québec à Montréal , P.O. Box 8888, Downtown Station, Montreal H3C 3P8, Canada.
authors.forename: Maïwenn
authors.initials: M
authors.lastname: Beaugrand
authors.affiliation: Département de Chimie, Université du Québec à Montréal , P.O. Box 8888, Downtown Station, Montreal H3C 3P8, Canada.
authors.forename: Alexandre A
authors.initials: AA
authors.lastname: Arnold
authors.affiliation: Département de Chimie, Université du Québec à Montréal , P.O. Box 8888, Downtown Station, Montreal H3C 3P8, Canada.
authors.forename: Antoine
authors.initials: A
authors.lastname: Juneau
authors.affiliation: Département de Chimie, Université du Québec à Montréal , P.O. Box 8888, Downtown Station, Montreal H3C 3P8, Canada.
authors.forename: Aline Balieiro
authors.initials: AB
authors.lastname: Gambaro
authors.affiliation: Département de Chimie, Université du Québec à Montréal , P.O. Box 8888, Downtown Station, Montreal H3C 3P8, Canada.|UMR 7099, CNRS - Université Paris Diderot, IBPC, 13 rue Pierre et Marie Curie, F-75005 Paris, France.
authors.forename: Dror E
authors.initials: DE
authors.lastname: Warschawski
authors.affiliation: Centre for Biological Sciences/Institute of Life Sciences, Highfield Campus, University of Southampton , Southampton SO17 1BJ, United Kingdom.
authors.forename: Philip T F
authors.initials: PT
authors.lastname: Williamson
authors.affiliation: Département de Chimie, Université du Québec à Montréal , P.O. Box 8888, Downtown Station, Montreal H3C 3P8, Canada.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Marcotte