title: Study the therapeutic mechanism of Amomum compactum in gentamicin-induced acute kidney injury rat based on a back propagation neural network algorithm.
abstract: Acute kidney injury (AKI) is a major global public health problems, as it causes high morbidity and serious injury to renal function. However, the etiology for AKI is not very clear. In this study, a serum metabolite profile analysis was performed to identify potential biomarkers for gentamicin-induced AKI and to investigate the mechanism of action of Amomum compactum (AC) used for treatment. A metabonomics approach by ultra-performance liquid chromatography together with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was applied to perform the analysis. Back propagation (BP) neural network models were established for classifying data from the control, model, and AC-treated groups. Accuracy rate for classification was 91.7% in positive ion mode and 87.5% in negative ion mode. By orthogonal partial least squares discriminant analysis (OPLS-DA), 29 metabolites were identified as potential biomarkers of gentamicin-induced AKI. Most of them are related to phospholipid metabolism. After treatment with AC, the levels of sphingomyelin, sphingosine, phytosphingosine, and arachidonic acid were restored to normal. The results indicate that AC plays a protective role in rats with gentamicin-induced AKI via regulation of the phospholipid metabolic pathway. In this work, early biomarkers of AKI has been identified and underlying therapeutic mechanism of AC has been understood, therefore, AC can be further investigated and tested for clinical application.
authors.affiliation: Inner Mongolia Key laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, College of Chemistry and Chemical engineering, Inner Mongolia University for the Nationalities, Tongliao, 028000, China.
authors.forename: Xiye
authors.initials: X
authors.lastname: Wang
authors.affiliation: Mongolian Medicine Engineering Technology Research Institute of Inner Mongolia, Tongliao, 028000, China; Mongolia Medical Department of Inner Mongolia University for the Nationalities, Tongliao, 028000, China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Chen
authors.affiliation: Mongolian Medicine Engineering Technology Research Institute of Inner Mongolia, Tongliao, 028000, China; Mongolia Medical Department of Inner Mongolia University for the Nationalities, Tongliao, 028000, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Chang
authors.affiliation: Key Laboratory for Symbol Computation and Knowledge Engineering of National Education Ministry, College of Computer Science and Technology, Jilin University, Changchun, 130012, China; College of Computer Science and Technology, Inner Mongolia University for the Nationalities, Tongliao, 028000, China.
authors.forename: Mingyang
authors.initials: M
authors.lastname: Jiang
authors.affiliation: Mongolian Medicine Engineering Technology Research Institute of Inner Mongolia, Tongliao, 028000, China; Mongolia Medical Department of Inner Mongolia University for the Nationalities, Tongliao, 028000, China. Electronic address: ld.312@163.com.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Inner Mongolia Key laboratory for the Natural Products Chemistry and Functional Molecular Synthesis, College of Chemistry and Chemical engineering, Inner Mongolia University for the Nationalities, Tongliao, 028000, China. Electronic address: mdxuliang@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Xu
mesh_terms: D058186:Acute Kidney Injury / Q000139:chemically induced / Q000188:drug therapy* / Q000378:metabolism* / Q000473:pathology; D035801:Amomum / Q000737:chemistry; D000818:Animals; D000900:Anti-Bacterial Agents; D015415:Biomarkers / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D016002:Discriminant Analysis; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000627:therapeutic use*; D005839:Gentamicins; D007668:Kidney / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008297:Male; D053858:Metabolic Networks and Pathways; D055432:Metabolomics / Q000379:methods*; D016571:Neural Networks, Computer; D010743:Phospholipids / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000900:Anti-Bacterial Agents; D015415:Biomarkers; D004365:Drugs, Chinese Herbal; D005839:Gentamicins; D010743:Phospholipids
keywords: Acute kidney injury; Amomum compactum; BP neural network algorithm; Gentamicin; Metabonomics; UPLC–MS