title: In vivo Assessment of Antioxidant and Wound Healing Improvement of a New Schiff Base Derived Co (II) Complex in Rats.
abstract: Co (II) complex (CMLA) was investigated to evaluate the rate of wound healing in rats. Animals were placed into four groups: gum acacia, Intrasite gel, 10 and 20 mg/ml of CMLA. Wounds were made on the dorsal neck area, then treated with Intrasite gel or CMLA; both of these treatments led to faster healing than with gum acacia. Histology of the wounds dressed with CMLA or Intrasite gel displayed a smaller scar width, required less time to heal and showed more collagen staining and fewer inflammatory cells in comparison to wounds dressed with the vehicle. Immunohistochemistry for Hsp70 and TGF-β showed greater staining intensity in the treated groups compared to the vehicle group. Bax staining was less intense in treated groups compared to the vehicle group, suggesting that CMLA and Intrasite gel provoked apoptosis, responsible for the development of granulation tissue into a scar. CD31 protein analysis showed that the treated groups enhanced angiogenesis and increased vascularization compared to the control group. Furthermore, a significant increase in the levels of GPx and SOD and a decrease in MDA were also observed in the treated groups. This results suggest that CMLA is a potentially promising agent for the wounds treatment.
authors.affiliation: Faculty of Science and Technology, Universiti Sains Islam Malaysia, 71800, Nilai, Negeri Sembilan DK, Malaysia.
authors.forename: Rashd M
authors.initials: RM
authors.lastname: El-Ferjani
authors.affiliation: Faculty of Science and Technology, Universiti Sains Islam Malaysia, 71800, Nilai, Negeri Sembilan DK, Malaysia.
authors.forename: Musa
authors.initials: M
authors.lastname: Ahmad
authors.affiliation: Department of Microbiology, Faculty of Medicine, University of Duhok, 78 Kurdistan, Iraq.
authors.forename: Summaya M
authors.initials: SM
authors.lastname: Dhiyaaldeen
authors.affiliation: Faculty of Science and Technology, Universiti Sains Islam Malaysia, 71800, Nilai, Negeri Sembilan DK, Malaysia.
authors.forename: Farah Wahida
authors.initials: FW
authors.lastname: Harun
authors.affiliation: Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.|Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mohamed Yousif
authors.initials: MY
authors.lastname: Ibrahim
authors.affiliation: School of Pharmacy, Ahfad University for Women (AUW), 167 Omdurman, Sudan.
authors.forename: Hoyam
authors.initials: H
authors.lastname: Adam
authors.affiliation: School of Chemical Sciences and Food Technology, Universiti Kebangsaan Malaysia, 43600, Bangi, Selangor D.E., Malaysia.
authors.forename: Bohari
authors.initials: B
authors.lastname: Mohd Yamin
authors.affiliation: Medical Microbiology Department, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Mazen M Jamil
authors.initials: MM
authors.lastname: Al-Obaidi
authors.affiliation: Medical Microbiology Department, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia.|Institute of Research Management &Monitoring, Deputy Vice Chancellor (Research and Innovation), University of Malaya, 50603 Kuala Lumpur, Malaysia.
authors.forename: Rami Al
authors.initials: RA
authors.lastname: Batran
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D003035:Cobalt / Q000737:chemistry / Q000494:pharmacology; D056831:Coordination Complexes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D051381:Rats; D017207:Rats, Sprague-Dawley; D012545:Schiff Bases / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D000975:Antioxidants; D056831:Coordination Complexes; D012545:Schiff Bases; D003035:Cobalt