title: Multiparametric photoacoustic microscopy of the mouse brain with 300-kHz A-line rate.
abstract: Enabling simultaneous high-resolution imaging of the total concentration of hemoglobin ([Formula: see text]), oxygen saturation of hemoglobin ([Formula: see text]), and cerebral blood flow (CBF), multiparametric photoacoustic microscopy (PAM) holds the potential to quantify the cerebral metabolic rate of oxygen at the microscopic level. However, its imaging speed has been severely limited by the pulse repetition rate of the dual-wavelength photoacoustic excitation and the scanning mechanism. To address these limitations, we have developed a new generation of multiparametric PAM. Capitalizing on a self-developed high-repetition dual-wavelength pulsed laser and an optical-mechanical hybrid-scan configuration, this innovative technique has achieved an unprecedented A-line rate of 300 kHz, leading to a 20-fold increase in the imaging speed over our previously reported multiparametric PAM that is based on pure mechanical scanning. The performance of the high-speed multiparametric PAM has been examined bothin vitroandin vivo. Simultaneous PAM of microvascular [Formula: see text], [Formula: see text], and CBF in absolute values over a [Formula: see text]-mm-diameter brain region of interest can be accomplished within 10 min.
authors.affiliation: University of Virginia , Department of Biomedical Engineering, 415 Lane Road, Charlottesville, Virginia 22908, United States.
authors.forename: Tianxiong
authors.initials: T
authors.lastname: Wang
authors.affiliation: University of Virginia , Department of Biomedical Engineering, 415 Lane Road, Charlottesville, Virginia 22908, United States.
authors.forename: Naidi
authors.initials: N
authors.lastname: Sun
authors.affiliation: University of Virginia , Department of Biomedical Engineering, 415 Lane Road, Charlottesville, Virginia 22908, United States.
authors.forename: Rui
authors.initials: R
authors.lastname: Cao
authors.affiliation: University of Virginia , Department of Biomedical Engineering, 415 Lane Road, Charlottesville, Virginia 22908, United States.
authors.forename: Bo
authors.initials: B
authors.lastname: Ning
authors.affiliation: University of Southern California , Department of Biomedical Engineering, 1042 Downey Way, Los Angeles, California 90089, United States.
authors.forename: Ruimin
authors.initials: R
authors.lastname: Chen
authors.affiliation: University of Southern California , Department of Biomedical Engineering, 1042 Downey Way, Los Angeles, California 90089, United States.
authors.forename: Qifa
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: University of Virginia , Department of Biomedical Engineering, 415 Lane Road, Charlottesville, Virginia 22908, United States.
authors.forename: Song
authors.initials: S
authors.lastname: Hu
keywords: cerebral hemodynamics; high-speed imaging; photoacoustic microscopy