title: A Mouse Model of Diet-Induced Obesity Resembling Most Features of Human Metabolic Syndrome.
abstract: Increased chicken-derived fat and fructose consumption in the human diet is paralleled by an increasing prevalence of obesity and metabolic syndrome (MS). Herein, we aimed at developing and characterizing a mouse model of diet-induced obesity (DIO) resembling most of the key features of the human MS. To accomplish this, we fed male C57BL/6J mice for 4, 8, 12, and 16 weeks with either a low-fat diet (LFD) or a high-chicken-fat diet (HFD) and tap water with or without 10% fructose (F). This experimental design resulted in the following four experimental groups: LFD, LFD + F, HFD, and HFD + F. Over the feeding period, and on a weekly basis, the HFD + F group had more caloric intake and gained more weight than the other experimental groups. Compared to the other groups, and at the end of the feeding period, the HFD + F group had a higher adipogenic index, total cholesterol, low-density lipoprotein cholesterol, fasting basal glycemia, insulin resistance, hypertension, and atherogenic index and showed steatohepatitis and systemic oxidative stress/inflammation. A mouse model of DIO that will allow us to study the effect of MS in different organs and systems has been developed and characterized.
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.; Laboratory of Experimental Therapeutics, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Maria C
authors.initials: MC
authors.lastname: Della Vedova
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Marcos D
authors.initials: MD
authors.lastname: Muñoz
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.; Laboratory of Experimental Therapeutics, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.; Institute for Biochemical Research (INBIO), San Luis, Argentina.
authors.forename: Lucas D
authors.initials: LD
authors.lastname: Santillan
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Maria G
authors.initials: MG
authors.lastname: Plateo-Pignatari
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Maria J
authors.initials: MJ
authors.lastname: Germanó
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Martín E
authors.initials: ME
authors.lastname: Rinaldi Tosi
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Silvina
authors.initials: S
authors.lastname: Garcia
authors.affiliation: Laboratory of Morphophysiology, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Nidia N
authors.initials: NN
authors.lastname: Gomez
authors.affiliation: LIAM, Andrology Research Laboratory from Mendoza, IHEM-CCT-Mendoza-National University of Cuyo, Mendoza, Argentina.
authors.forename: Miguel W
authors.initials: MW
authors.lastname: Fornes
authors.affiliation: Laboratory of Experimental Therapeutics, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Sandra E
authors.initials: SE
authors.lastname: Gomez Mejiba
authors.affiliation: Laboratory of Experimental and Translational Medicine, IMIBIO-SL, CONICET, National University of San Luis, San Luis, Argentina.
authors.forename: Dario C
authors.initials: DC
authors.lastname: Ramirez
keywords: adiposity; diet-induced obesity; inflammation; metabolic syndrome; mouse model; redox change