title: Wastes valorization from Rhodosporidium toruloides NCYC 921 production and biorefinery by anaerobic digestion.
abstract: Yeast production and biomass biorefinery processes for lipid and carotenoid extraction generate residues that can be used as substrates for anaerobic digestion. Glucose and carob pulp syrups were used as carbon sources to produce the yeast biomass. The yeast cultivation broth, yeast biomass residues (after carotenoid and lipid extraction) and the carob pulp solid residues obtained from the extraction of sugars were used to produce biogas by applying different Substrate/Inoculum ratios (S/I of 0.5 and 0.75). For all the residues studied, the digestions at the S/I ratio of 0.75 provided higher biogas yields than those carried out at the S/I ratio of 0.5. The best results in terms of biogas production and methane yield were observed for the yeast residue digestion at S/I of 0.75 (65.9mL, 333.7mLg-1VS-1substrate). As monitored through flow cytometry, its bacterial consortium showed the lowest proportion of injured cells.
authors.affiliation: Laboratório Nacional de Energia e Geologia, Unidade de Bioenergia, Estrada do Paço do Lumiar, 1649-038 Lisboa, Portugal; LEAF-Linking Landscape, Environment, Agriculture and Food, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada da Ajuda, 1349-017 Lisboa, Portugal.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Batista
authors.affiliation: Laboratório Nacional de Energia e Geologia, Unidade de Bioenergia, Estrada do Paço do Lumiar, 1649-038 Lisboa, Portugal.
authors.forename: Emílio Palomo
authors.initials: EP
authors.lastname: López
authors.affiliation: Laboratório Nacional de Energia e Geologia, Unidade de Bioenergia, Estrada do Paço do Lumiar, 1649-038 Lisboa, Portugal.
authors.forename: Carla
authors.initials: C
authors.lastname: Dias
authors.affiliation: Laboratório Nacional de Energia e Geologia, Unidade de Bioenergia, Estrada do Paço do Lumiar, 1649-038 Lisboa, Portugal. Electronic address: teresa.lopessilva@lneg.pt.
authors.forename: Teresa
authors.initials: T
authors.lastname: Lopes da Silva
authors.affiliation: Laboratório Nacional de Energia e Geologia, Unidade de Bioenergia, Estrada do Paço do Lumiar, 1649-038 Lisboa, Portugal.
authors.forename: Isabel Paula
authors.initials: IP
authors.lastname: Marques
mesh_terms: D000693:Anaerobiosis; D001487:Basidiomycota / Q000254:growth & development* / Q000378:metabolism; D061249:Batch Cell Culture Techniques; D056804:Biofuels; D057919:Biological Oxygen Demand Analysis; D018533:Biomass; D019149:Bioreactors; D001709:Biotechnology / Q000295:instrumentation / Q000379:methods*; D002244:Carbon / Q000378:metabolism; D002338:Carotenoids / Q000096:biosynthesis / Q000302:isolation & purification; D005685:Galactans / Q000737:chemistry; D008055:Lipids / Q000096:biosynthesis / Q000302:isolation & purification; D008351:Mannans / Q000737:chemistry; D008697:Methane / Q000096:biosynthesis*; D053149:Plant Gums / Q000737:chemistry
chemical_list: D056804:Biofuels; D005685:Galactans; D008055:Lipids; D008351:Mannans; D053149:Plant Gums; D002338:Carotenoids; D002244:Carbon; D008697:Methane; C017471:locust bean gum
keywords: Biogas production; Flow cytometry; Yeast biorefinery; Yeast production