title: Identification of differentially methylated regions (DMRs) of neuronatin in mice.
abstract: BACKGROUND
  Neuronatin (NNAT) is a paternal-inherited imprinted gene, first discovered in the rat neonatal brain, where it plays vital roles for neuronal growth, brain development, and metabolic regulation. The maternal imprint ofNNAThas been identified in mice; however, the differentially methylated regions (DMRs) involved in the monoallelic expression ofNNAThave not yet been investigated.
  
  
  RESULTS
  In this study, we confirmed expression of two isoforms of theNNAT(α and β) in the mice brain via quantitative RT-PCR. Additionally, the methylation profile of the CpG island located in theNNATgene locus was determined in the mice liver, brain, sperm, and the MII oocyte via bisulfite sequencing PCR.
  
  
  CONCLUSIONS
  In summary, we provide the first evidence for tissue- and gamete-specific methylation patterns of CpG3 that are located on exon 1, to be putative DMR ofNNATin mice.
authors.affiliation: College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
authors.forename: Yuxin
authors.initials: Y
authors.lastname: Xu
authors.affiliation: College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
authors.forename: Tiedong
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
authors.forename: Xianju
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
authors.forename: Jichao
authors.initials: J
authors.lastname: Deng
authors.affiliation: College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
authors.forename: Mao
authors.initials: M
authors.lastname: Chen
authors.affiliation: College of Animal Science, Jilin University, 5333#, Xi'an Road, Changchun, 130062 China.
authors.forename: Zhanjun
authors.initials: Z
authors.lastname: Li
keywords: DMR; Isoform; Mice; NNAT