title: Cytotoxicity Evaluation of Two Bis-Acryl Composite Resins Using Human Gingival Fibroblasts.
abstract: Bis-acryl resins are used for temporary dental restorations and have shown advantages over other materials. The aim of this work was to evaluate the in vitro cytotoxicity of two bis-acryl composite resins (Protemp 4 and Luxatemp Star), obtained at 1, 7 and 40 days after mixing the resin components, using a standardized assay employing human primary cells closely related to oral tissues. Human gingival fibroblast cell cultures were exposed for 24 h to either bis-acryl composite resins, polystyrene beads (negative control) and latex (positive control) extracts obtained after incubation by the different periods, at 37 °C under 5% CO2. Cell viability was evaluated using a multiparametric procedure involving sequential assessment (using the same cells) of mitochondrial activity (XTT assay), membrane integrity (neutral red test) and total cell density (crystal violet dye exclusion test). The cells exposed to the resin extracts showed cell viability indexes exceeding 75% after 24 h. Even when cells were exposed to extracts prepared with longer conditioning times, the bis-acryl composite resins showed no significant cytotoxic effects (p>0.05), compared to the control group or in relation to the first 24 h of contact with the products. There were no differences among the results obtained for the bis-acryl composite resins evaluated 24 h, 7 days and 40 days after mixing. It may be concluded that the bis-acryl resins Protemp 4 and Luxatemp Star were cytocompatible with human gingival fibroblasts, suggesting that both materials are suitable for use in contact with human tissues.
authors.affiliation: Department of Clinical Dentistry, Laboratory of Experimental Cell Culture (LECCel), UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Fabiano Palmeira
authors.initials: FP
authors.lastname: Gonçalves
authors.affiliation: Hospital Antônio Pedro, Molecular and Cell Biology Department, UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Gutemberg
authors.initials: G
authors.lastname: Alves
authors.affiliation: Department of Clinical Dentistry, Laboratory of Experimental Cell Culture (LECCel), UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Vladi Oliveira
authors.initials: VO
authors.lastname: Guimarães
authors.affiliation: Department of Clinical Dentistry, Laboratory of Experimental Cell Culture (LECCel), UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Marco Antônio
authors.initials: MA
authors.lastname: Gallito
authors.affiliation: Department of Clinical Dentistry, Laboratory of Experimental Cell Culture (LECCel), UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Felipe
authors.initials: F
authors.lastname: Oliveira
authors.affiliation: Department of Clinical Dentistry, Laboratory of Experimental Cell Culture (LECCel), UFF - Universidade Federal Fluminense, Niterói, RJ, Brazil.
authors.forename: Míriam Zaccaro
authors.initials: MZ
authors.lastname: Scelza
mesh_terms: D003188:Composite Resins; D005347:Fibroblasts / Q000166:cytology; D005881:Gingiva / Q000166:cytology*; D006801:Humans
chemical_list: D003188:Composite Resins