title: Identification and genomic analysis of antifungal property of a tomato root endophyte Pseudomonas sp. p21.
abstract: Pseudomonas sp., which occupy a variety of ecological niches, have been widely studied for their versatile metabolic capacity to promote plant growth, suppress microbial pathogens, and induce systemic resistance in plants. In this study, a Pseudomonas sp. strain p21, which was isolated from tomato root endophytes, was identified as having antagonism against Aspergillus niger. Further analysis showed that this strain had the ability to biosynthesise siderophores and was less effective in inhibiting the growth of A. niger with the supplementation of Fe3+in the agar medium. Genomic sequencing and the secondary metabolite cluster analysis demonstrated that Pseudomonas sp. p21 harboured 2 pyoverdine biosynthetic gene clusters, which encode compounds with predicted core structures and two variable tetra-peptide or eleven-peptide chains. The results indicated that siderophore-mediated competition for iron might be an important mechanism in Pseudomonas suppression of the fungal pathogen A. niger and in microbe-pathogen-plant interactions.
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou, 350108, Fujian, China.
authors.forename: Rongqin
authors.initials: R
authors.lastname: Ma
authors.affiliation: Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Science, Guiyang, 550081, Guizhou, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou, 350108, Fujian, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou, 350108, Fujian, China.
authors.forename: Shaonan
authors.initials: S
authors.lastname: Lei
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou, 350108, Fujian, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Quality Standards and Testing Technology for Agro-Products, Fujian Academy of Agricultural Sciences, Fuzhou, 350003, Fujian, China.
authors.forename: Yongkang
authors.initials: Y
authors.lastname: Song
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, and College of Life Sciences, Fujian Normal University, Fuzhou, 350108, Fujian, China. tianby@fjnu.edu.cn.
authors.forename: Baoyu
authors.initials: B
authors.lastname: Tian
mesh_terms: D000898:Antibiosis / Q000235:genetics; D001234:Aspergillus niger / Q000502:physiology; D060026:Endophytes / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D023281:Genomics; D054884:Host-Pathogen Interactions; D018551:Solanum lycopersicum / Q000382:microbiology*; D009842:Oligopeptides / Q000096:biosynthesis / Q000737:chemistry / Q000235:genetics; D010935:Plant Diseases / Q000382:microbiology; D018517:Plant Roots / Q000382:microbiology; D011549:Pseudomonas / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017262:Siderophores / Q000096:biosynthesis / Q000494:pharmacology
chemical_list: D009842:Oligopeptides; D012336:RNA, Ribosomal, 16S; D017262:Siderophores; C042453:pyoverdin
keywords: Antifungal activity; Genomic analysis; Microbe-pathogen-plant interaction; Pseudomonas; Siderophore pyoverdine