title: Wound-healing promoting effect of total tannins from Entada phaseoloides (L.) Merr. in rats.
abstract: The healing of wounds has always provided challenges for the medical community whether chronic or acute. Modern and traditional medicine has proved that herbal medicine shown superiority over chemical drugs. Herein, we report an Entada phaseoloides (L.) Merr. extract with a total tannin content of 76.18% showed wound-healing promoting effect in rat model. We found significantly accelerated wound closure already on day 7 in animals treated with total Entada phaseoloides (L.) Merr. tannins (TEPT) as compared to vaseline treated controls (p<0.05). At day 15, histologically, the wounds in animals treated with TEPT were completely closed as compared to controls. In vitro, TEPT promotes fibroblast proliferation and migration into wounds of NIH3T3 with concentration range of 9.38-37.50μg/ml. TEPT also had an inhibitory action against Staphylococcus aureus with MBC of 1.5mg/ml and the result was further proved by transmission electron microscope. Thus, TEPT could promote wound shrinkage, improve healing rate and promote healing of infectious wounds in rats. And this effect may due to antibacterial activities and NIH3T3 cell pro-proliferative effect of the tannins compounds, which indicating that TEPT can be used as efficient treatment in traumatic injury.
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian, PR China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Su
authors.affiliation: Institute/College of Integrative Medicine, Dalian Medical University, Dalian, PR China.
authors.forename: Xinguang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Institute/College of Integrative Medicine, Dalian Medical University, Dalian, PR China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: Institute/College of Integrative Medicine, Dalian Medical University, Dalian, PR China.
authors.forename: Taowen
authors.initials: T
authors.lastname: Pan
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian, PR China.
authors.forename: Dingrui
authors.initials: D
authors.lastname: Liu
authors.affiliation: Liaoning University of Traditional Chinese Medicine, Dalian, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Dalian Medical University, Dalian, PR China. Electronic address: dydiaodiao@126.com.com.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Diao
authors.affiliation: College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, PR China. Electronic address: lslikun@163.com.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects*; D003094:Collagen / Q000096:biosynthesis / Q000187:drug effects; D004643:Emollients / Q000494:pharmacology; D007887:Fabaceae; D008297:Male; D051379:Mice; D046529:Microscopy, Electron, Transmission; D016712:Mupirocin / Q000494:pharmacology; D041681:NIH 3T3 Cells; D010577:Petrolatum / Q000494:pharmacology; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012867:Skin / Q000187:drug effects* / Q000293:injuries / Q000473:pathology / Q000648:ultrastructure; D013203:Staphylococcal Infections; D013211:Staphylococcus aureus / Q000187:drug effects*; D013634:Tannins / Q000494:pharmacology*; D014945:Wound Healing / Q000187:drug effects*; D014946:Wound Infection
chemical_list: D000900:Anti-Bacterial Agents; D004643:Emollients; D010936:Plant Extracts; D013634:Tannins; D010577:Petrolatum; D003094:Collagen; D016712:Mupirocin
keywords: Antibacteria; Entada phaseoloides (L.) Merr.; Total tannin; Wound healing