title: Inhibition by Multifunctional Magnetic Nanoparticles Loaded with Alpha-Synuclein RNAi Plasmid in a Parkinson's Disease Model.
abstract: Lewy bodies are considered as the main pathological characteristics of Parkinson's disease (PD). The major component of Lewy bodies is α-synuclein (α-syn). The use of gene therapy that targeting and effectively interfere with the expression of α-syn in neurons has received tremendous attention. In this study, we used magnetic Fe3O4nanoparticles coated with oleic acid molecules as a nano-carrier.N-isopropylacrylamide derivative (NIPAm-AA) was photo-immobilized onto the oleic acid molecules, and shRNA (short hairpin RNA) was absorbed. The same method was used to absorb nerve growth factor (NGF) to NIPAm-AA to specifically promote neuronal uptake via NGF receptor-mediated endocytosis. Additionally, shRNA plasmid could be released into neurons because of the temperature and pH sensitivity of NIPAm-AA interference with α-syn synthesis. We investigated apoptosis in neurons with abrogated α-syn expressionin vitroandin vivo. The results demonstrated that multifunctional superparamagnetic nanoparticles carrying shRNA for α-syn could provide effective repair in a PD model.
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
authors.forename: Shuiqin
authors.initials: S
authors.lastname: Niu
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Ling-Kun
authors.initials: LK
authors.lastname: Zhang
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Siyi
authors.initials: S
authors.lastname: Zhuang
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Xiuyu
authors.initials: X
authors.lastname: Zhan
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Wu-Ya
authors.initials: WY
authors.lastname: Chen
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Shiwei
authors.initials: S
authors.lastname: Du
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yin
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Rong
authors.initials: R
authors.lastname: You
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.
authors.forename: Chu-Hua
authors.initials: CH
authors.lastname: Li
authors.affiliation: School of Life Science, South China Normal University, Guangzhou 510631, China.; MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, 510631, China.
authors.forename: Yan-Qing
authors.initials: YQ
authors.lastname: Guan
mesh_terms: D000178:Acrylamides / Q000008:administration & dosage; D000818:Animals; D001688:Biological Products / Q000008:administration & dosage*; D004195:Disease Models, Animal; D004337:Drug Carriers / Q000008:administration & dosage; D004705:Endocytosis; D015316:Genetic Therapy / Q000379:methods*; D058185:Magnetite Nanoparticles / Q000008:administration & dosage*; D008297:Male; D008810:Mice, Inbred C57BL; D020932:Nerve Growth Factor / Q000008:administration & dosage; D009474:Neurons / Q000502:physiology; D010300:Parkinson Disease / Q000628:therapy*; D010957:Plasmids / Q000008:administration & dosage*; D034741:RNA, Small Interfering / Q000008:administration & dosage* / Q000235:genetics; D051844:alpha-Synuclein / Q000037:antagonists & inhibitors* / Q000235:genetics
chemical_list: D000178:Acrylamides; D001688:Biological Products; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D034741:RNA, Small Interfering; D051844:alpha-Synuclein; D020932:Nerve Growth Factor; C067295:N-isopropylacrylamide
keywords: Fe3O4 nanoparticles; Parkinson's disease.; RNAi; shRNA; α-synuclein