title: Experimental immunological demyelination enhances regeneration in autograft-repaired long peripheral nerve gaps.
abstract: Peripheral nerve long gap defects are a clinical challenge in the regeneration field. Despite the wide variety of surgical techniques and therapies, autografting is the "gold standard" for peripheral nerve gap reconstruction. The pathological process of Wallerian degeneration from the time of acute injury to efficient regeneration requires several weeks. Regeneration time is critical for nerve reconstruction. Immunological demyelination induced by anti-galactocerebroside antibodies plus guinea pig complement was used to shorten the treatment time. Based on an antigen-antibody complex reaction, the demyelinating agent induced an acute and severe demyelination, leading to the pathological process of Wallerian degeneration during the demyelinating period. This method was used to treat a 12 mm-long sciatic nerve defect in rats. The control groups were injected with one of the demyelinating agent components. The results indicated that anti-galactocerebroside antibodies plus guinea pig complement can significantly shorten treatment time and promote nerve regeneration and functional recovery. In addition, the demyelinating agent can increase the mRNA levels of nerve growth factors and can regulate inflammation. In conclusion, treatment with anti-galactocerebroside antibodies plus guinea pig complement can promote axonal regeneration. This therapy provides a novel method to improve functional recovery in the treatment of long nerve defects.
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.|The department of anatomy, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Ge
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Yafeng
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Zhongyang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Xueyu
authors.initials: X
authors.lastname: Hu
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Quan
authors.affiliation: General Political Department Hospital of PLA, Beijing 100120, PR China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Jinghui
authors.initials: J
authors.lastname: Huang
authors.affiliation: The department of anatomy, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Yunqing
authors.initials: Y
authors.lastname: Li
authors.affiliation: Institute of Orthopedics, Xijing Hospital, the Fourth Military Medical University, Xi'an 710032, PR China.
authors.forename: Zhuojing
authors.initials: Z
authors.lastname: Luo
mesh_terms: D000818:Animals; D020278:Demyelinating Autoimmune Diseases, CNS / Q000276:immunology*; D005260:Female; D006168:Guinea Pigs; D009416:Nerve Regeneration / Q000276:immunology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology*; D014855:Wallerian Degeneration / Q000276:immunology*