title: Single-cell analysis of differences in transcriptomic profiles of oocytes and cumulus cells at GV, MI, MII stages from PCOS patients.
abstract: Polycystic ovary syndrome (PCOS) is a common frequent endocrine disorder among women of reproductive age. Although assisted reproductive techniques (ARTs) are used to address subfertility in PCOS women, their effectiveness is not clear. Our aim was to compare transcriptomic profiles of oocytes and cumulus cells (CCs) between women with and without PCOS, and assess the effectiveness of ARTs in treating PCOS patients. We collected oocytes and CCs from 16 patients with and without PCOS patients to categorize them into 6 groups according to oocyte nuclear maturation. Transcriptional gene expression of oocyte and CCs was determined via single-cell RNA sequencing. The ratio of fertilization and cleavage was higher in PCOS patients than in non-PCOS patients undergoing ARTs, and there was no difference in the number of high-quality embryos between the groups. Differentially expressed genes including PPP2R1A, PDGFRA, EGFR, GJA1, PTGS2, TNFAIP6, TGF-β1, CAV1, INHBB et al. were investigated as potential causes of PCOS oocytes and CCs disorder at early stages, but their expression returned to the normal level at the metaphase II (MII) stage via ARTs. In conclusion, ARTs can improve the quality of cumulus-oocyte complex (COC) and increase the ratio of fertilization and cleavage in PCOS women.
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Tongji Hospital, Tongji University, Shanghai, China.
authors.forename: Qiwei
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Reproductive Medical Center of XiangYa Hospital, Affiliated Central South University, Changsha, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Translational Center for Stem Cell Research, Tongji Hospital, Department of Regenerative Medicine, Tongji University, Shanghai, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Translational Center for Stem Cell Research, Tongji Hospital, Department of Regenerative Medicine, Tongji University, Shanghai, China.
authors.forename: Chaojie
authors.initials: C
authors.lastname: Liu
authors.affiliation: Translational Center for Stem Cell Research, Tongji Hospital, Department of Regenerative Medicine, Tongji University, Shanghai, China.
authors.forename: Jieliang
authors.initials: J
authors.lastname: Ma
authors.affiliation: Translational Center for Stem Cell Research, Tongji Hospital, Department of Regenerative Medicine, Tongji University, Shanghai, China.
authors.forename: Yifei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Xiang
authors.affiliation: Reproductive Medicine Center, Affiliated Tongji Hospital, Tongji University, Shanghai, China.
authors.forename: Yazhong
authors.initials: Y
authors.lastname: Ji
authors.affiliation: Reproductive Medicine Center, The First Affiliated Hospital of Anhui Medical University, Hefei, China.
authors.forename: Yunxia
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Gynecology and Obstetrics, Affiliated Tongji Hospital, Tongji University, Shanghai, China.
authors.forename: Xiaowen
authors.initials: X
authors.lastname: Tong
authors.affiliation: Translational Center for Stem Cell Research, Tongji Hospital, Department of Regenerative Medicine, Tongji University, Shanghai, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Xue
mesh_terms: D000328:Adult; D015992:Body Mass Index; D054885:Cumulus Cells / Q000166:cytology / Q000378:metabolism*; D005260:Female; D017629:Gap Junctions / Q000473:pathology; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008677:Metaphase; D009865:Oocytes / Q000166:cytology / Q000378:metabolism*; D009866:Oogenesis; D018384:Oxidative Stress; D011085:Polycystic Ovary Syndrome / Q000378:metabolism*; D027724:Reproductive Techniques, Assisted; D017423:Sequence Analysis, RNA; D015398:Signal Transduction; D059010:Single-Cell Analysis; D059467:Transcriptome