title: [Relationship between urinary polycyclic aromatic hydrocarbon metabolite and cell cycle of lymphocyte in coke oven workers].
abstract: Objective:To investigate the relationship between urinary polycyclic aromatic hydrocarbon metabolite and cell cycle of lymphocyte in coke oven workers.Methods:437 coke oven workers and 163 work-ers in water treatment department were recruited in this study. Flow cytometry was used to detect the cell cycle of lymphocyte. For the measurement of urinary metabolites, urine samples were treated with β-glucuronidase and analyzed using HPLC with a fluorescence detector.Results:The concentrations of urinary 2-naphthol, 2-hydroxyfluorene, 9-phenanthrol and 1-hydroxypyrene l in coke oven workers were significantly higher than those in control group (P<0.01) . The distributions of cell cycle were analyzed in high exposure group (the content of urinary metabolites high thanP75) and low exposure group (the content of urinary metabolites low thanP25) . According to the content of 1-hydroxypyrene, the proportions of S phase in high exposure group were significant-ly higher than those of low exposure group (Z=-2.496,P=0.013) , but the proportions of G0/G1 phase were sig-nificantly lower than low exposure group (Z=-2.074,P=0.038) . The similar results were not been found in other hydroxylated metabolites as internal exposure group.Conclusion:Increasing levels of urinary 1-hydroxypyrene might resulting in cell cycle of lymphocyte disorders, mainly for G0/G1 phase shorten and S phase arrest.
authors.affiliation: Department of Occupational Health, School of Public Health, Shanxi Medical University, Taiyuan 030001, China.
authors.forename: B L
authors.initials: BL
authors.lastname: Pan
authors.forename: H T
authors.initials: HT
authors.lastname: Zhang
authors.forename: H J
authors.initials: HJ
authors.lastname: Zhang
authors.forename: W T
authors.initials: WT
authors.lastname: Chen
authors.forename: J
authors.initials: J
authors.lastname: Yang
mesh_terms: D000328:Adult; D002455:Cell Division; D003077:Coke; D005449:Fluorenes; D006801:Humans; D008214:Lymphocytes; D008297:Male; D009284:Naphthols; D016273:Occupational Exposure; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons / Q000378:metabolism*; D011721:Pyrenes
chemical_list: D003077:Coke; D005449:Fluorenes; D009284:Naphthols; D010616:Phenanthrenes; D011084:Polycyclic Aromatic Hydrocarbons; D011721:Pyrenes; C091375:9-phenanthrol; C477779:2-hydroxyfluorene; C033146:1-hydroxypyrene; C028405:2-naphthol