title: Comparison and evaluation of antimuscarinic and anti-inflammatory effects of five Bulbus fritillariae species based on UPLC-Q/TOF integrated dual-luciferase reporter assay, PCA and ANN analysis.
abstract: Many species of Bulbus fritillariae are used as traditional medicines for thousands of years; however, their application is not standardized. To clarify the differences and homologies, the antimuscarinic and anti-inflammatory effects of five BM species were firstly tested and compared at cellular level. With an integrated strategy combining UPLC-Q/TOF MS, PCA and ANN analysis, the active ingredients among 28 different chemical markers were predicted and identified. SB and QB extracts showed the best antimuscarinic effects and several steroidal alkaloids, such as solanidine, contributed to this effects. However, ZB was superior to reduce the inflammatory response. Another five components were responsible by decreasing the expression of NF-κB, including puqiedine, zhepeiresinol, 2-monopalmitin, N-demethylpuqietinone, and isoverticine. More novelty, a new cluster of five BM species based on active ingredients as potential quality markers was depicted to illustrate their functions. These results of the study could make a reference for the medicinal application of BM species in clinic; and the integrated strategy provided an effective method to obtain the quality markers from medical herbs, which was helpful for the quality control of traditional medicinal products.
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
authors.forename: Mengge
authors.initials: M
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
authors.forename: Xiaoyao
authors.initials: X
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
authors.forename: Zengyong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: No. 6 TCM Factory, Zhongxin Pharmaceuticals, Tianjin 300193, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: No. 6 TCM Factory, Zhongxin Pharmaceuticals, Tianjin 300193, People's Republic of China.
authors.forename: Yongling
authors.initials: Y
authors.lastname: Tong
authors.affiliation: No. 6 TCM Factory, Zhongxin Pharmaceuticals, Tianjin 300193, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Hou
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address: minjiang@nankai.edu.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Medicinal Chemical Biology and College of Pharmacy, Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin 300350, People's Republic of China. Electronic address: gangbai@nankai.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Bai
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004144:Diosgenin; D031410:Fritillaria / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008156:Luciferases / Q000378:metabolism; D013058:Mass Spectrometry; D062105:Molecular Docking Simulation; D018727:Muscarinic Antagonists / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D016328:NF-kappa B / Q000032:analysis / Q000037:antagonists & inhibitors* / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D025341:Principal Component Analysis; D043585:Receptor, Muscarinic M2 / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism; D015203:Reproducibility of Results
chemical_list: D000893:Anti-Inflammatory Agents; D015415:Biomarkers; D018727:Muscarinic Antagonists; D016328:NF-kappa B; D010936:Plant Extracts; D043585:Receptor, Muscarinic M2; D008156:Luciferases; D004144:Diosgenin; C013255:solanidine
keywords: Bulbus fritillariae; M(2)R antagonists; NF-κB inhibitors; Quality markers; UPLC-Q/TOF MS