title: iTRAQ-based Quantitative Proteomics Study in Patients with Refractory Mycoplasma pneumoniae Pneumonia.
abstract: Mycoplasma pneumoniae (MP) is a leading cause of community-acquired pneumonia in children and young adults. Although MP pneumonia is usually benign and self-limited, in some cases it can develop into life-threating refractory MP pneumonia (RMPP). However, the pathogenesis of RMPP is poorly understood. The identification and characterization of proteins related to RMPP could provide a proof of principle to facilitate appropriate diagnostic and therapeutic strategies for treating paients with MP. In this study, we used a quantitative proteomic technique (iTRAQ) to analyze MP-related proteins in serum samples from 5 patients with RMPP, 5 patients with non-refractory MP pneumonia (NRMPP), and 5 healthy children. Functional classification, sub-cellular localization, and protein interaction network analysis were carried out based on protein annotation through evolutionary relationship (PANTHER) and Cytoscape analysis. A total of 260 differentially expressed proteins were identified in the RMPP and NRMPP groups. Compared to the control group, the NRMPP and RMPP groups showed 134 (70 up-regulated and 64 down-regulated) and 126 (63 up-regulated and 63 down-regulated) differentially expressed proteins, respectively. The complex functional classification and protein interaction network of the identified proteins reflected the complex pathogenesis of RMPP. Our study provides the first comprehensive proteome map of RMPP-related proteins from MP pneumonia. These profiles may be useful as part of a diagnostic panel, and the identified proteins provide new insights into the pathological mechanisms underlying RMPP.
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
authors.forename: Jia-Lu
authors.initials: JL
authors.lastname: Yu
authors.affiliation: Department of Bioengineering, Guangdong Province Key Laboratory of Molecular Immunology and Antibody Engineering, Jinan University.
authors.forename: Qi-Fang
authors.initials: QF
authors.lastname: Song
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: Xie
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
authors.forename: Wen-Hui
authors.initials: WH
authors.lastname: Jiang
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
authors.forename: Jia-Hui
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
authors.forename: Hui-Feng
authors.initials: HF
authors.lastname: Fan
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
authors.forename: Ya-Ping
authors.initials: YP
authors.lastname: Xie
authors.affiliation: Guangzhou Women and Children's Medical Center, Guangzhou Medical University.
authors.forename: Gen
authors.initials: G
authors.lastname: Lu
mesh_terms: D002648:Child; D002675:Child, Preschool; D005260:Female; D054884:Host-Pathogen Interactions; D006801:Humans; D007223:Infant; D008297:Male; D009177:Mycoplasma pneumoniae / Q000254:growth & development*; D011019:Pneumonia, Mycoplasma / Q000473:pathology*; D060066:Protein Interaction Maps; D020543:Proteome / Q000032:analysis*; D040901:Proteomics / Q000379:methods; D044967:Serum / Q000737:chemistry*
chemical_list: D020543:Proteome
keywords: Differentially expressed proteins; Quantitative proteomics; Refractory Mycoplasma pneumoniae pneumonia