title: Diuretic effect of extracts, fractions and two compounds 2α,3β,19α-trihydroxy-urs-12-en-28-oic acid and 5-hydroxy-3,6,7,8,4'-pentamethoxyflavone from Rubus rosaefolius Sm. (Rosaceae) leaves in rats.
abstract: Although diuretics have been widely used to treat hypertension along with others cardiovascular and renal disorders, no scientific data have been recorded to support the diuretic properties of Rubus rosaefolius Sm. (Rosaceae), a plant popularly used in Brazil to treat hypertension. Male Wistar rats were orally treated with: vehicle; hydrochlorothiazide; aqueous (AERR) and methanolic (MERR) extracts; dichloromethane (DCM), hexane (HEX) and ethyl acetate (EA) fractions; and the isolated compounds 2α,3β,19α-trihydroxy-urs-12-en-28-oic acid (TUA) and 5-hydroxy-3,6,7,8,4'-pentamethoxyflavone (PMF). At the end of the experiment (after 8 or 24 h), urine volume and other urine or plasma parameters were measured. AERR and MERR, at 100 and 30 mg/kg, respectively, induced diuretic, natriuretic and kaliuretic effect. Additionally, the DCM and HEX, but not EA, at 10 mg/kg, also increased urine volume and Na+and K+excretion. Both active constituents, TUA and PMF, at doses of 1 and 3 mg/kg, showed an augmented diuretic and natriuretic index. While TUA revealed a kaliuretic action, PMF did not interfere with potassium excretion. The compounds increased urinary creatinine, but not urea, levels. TUA was able to decrease calcium excretion, as well as HCTZ, while PMF effect was associated with increased urinary prostaglandin E2levels. The non-selective muscarinic receptor antagonist (atropine) prevented TUA-induced diuresis. In addition, indomethacin (a cyclooxygenase inhibitor) and atropine, exhibited the ability to block the diuretic effects prompted by PMF. Our study demonstrates the diuretic effect of extracts, fractions and two natural compounds obtained from R. rosaefolius leaves in rats.
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil. priscilasouza_rc@yahoo.com.br.
authors.forename: Priscila
authors.initials: P
authors.lastname: de Souza
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Thaise
authors.initials: T
authors.lastname: Boeing
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Lincon Bordignon
authors.initials: LB
authors.lastname: Somensi
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Camile Cecconi
authors.initials: CC
authors.lastname: Cechinel-Zanchett
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo (USP), Ribeirão Preto, SP, 14040-903, Brazil.
authors.forename: Jairo Kenupp
authors.initials: JK
authors.lastname: Bastos
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Marcel
authors.initials: M
authors.lastname: Petreanu
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Rivaldo
authors.initials: R
authors.lastname: Niero
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Valdir
authors.initials: V
authors.lastname: Cechinel-Filho
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Luisa Mota
authors.initials: LM
authors.lastname: da Silva
authors.affiliation: Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Rua Uruguai, 458, Itajaí, SC, 88302-901, Brazil.
authors.forename: Sérgio Faloni
authors.initials: SF
authors.lastname: de Andrade
mesh_terms: D000818:Animals; D001285:Atropine / Q000494:pharmacology; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D015232:Dinoprostone / Q000652:urine; D004232:Diuretics / Q000494:pharmacology*; D047309:Flavones / Q000494:pharmacology*; D007213:Indomethacin / Q000494:pharmacology; D008297:Male; D018727:Muscarinic Antagonists / Q000494:pharmacology; D019331:NG-Nitroarginine Methyl Ester / Q000494:pharmacology; D019001:Nitric Oxide Synthase / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves; D017208:Rats, Wistar; D065856:Rubus; D014315:Triterpenes / Q000494:pharmacology*
chemical_list: C435248:2,3,19-trihydroxy-12-oleanen-28-oic acid; C000617114:5-hydroxy-3,6,7,8,4'-pentamethoxyflavone; D016861:Cyclooxygenase Inhibitors; D004232:Diuretics; D047309:Flavones; D018727:Muscarinic Antagonists; D010936:Plant Extracts; D014315:Triterpenes; D001285:Atropine; D019001:Nitric Oxide Synthase; D015232:Dinoprostone; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin
keywords: Calcium; Creatinine; Flavonoids; Folk medicine; Prostaglandin; Triterpenes