title: Comparisons of the effects of the sevoflurane and propofol on acute ischemia reperfusion and DNA damages in rabbits.
abstract: OBJECTIVE
  The aim of this study was to compare the effects of sevoflurane and propofol anesthesia on oxidative DNA damage that occurs in low-extremity ischemia and is caused by tourniquet application.
  
  
  METHODS
  Fourteen New Zealand rabbits were randomly allocated into two equal groups. Group S (n=7) received sevoflurane (2.5-4 percent) inhalation and Group P (n=7) received a propofol infusion (1-2mg·kg-1·min-1), after which a pneumatic tourniquet was placed on the right lower extremity. Blood samples were collected prior to tourniquet placement (baseline), 120min after ischemia, 15min after ischemia and 120minutes (min) after ischemia. Malondialdehyde (MDA) levels were analyzed to determine lipid peroxidation, and single cell gel electrophoresis (SCGE) was used to determine DNA damage.
  
  
  RESULTS
  At 15min after ischemia, the MDA levels in Group P (8.15±2.61μM) were higher than baseline (6.26±3.19μM, p=0.026) and Group S (4.98±0.77μM, p=0.01). DNA damage was similar in both groups, although DNA damage was higher than baseline (tail moment 0.63±0.27, tail intensity 3.76±1.26) in Group P at the 15th minute of reperfusion (tail moment 1.05±0.45, p=0.06; tail intensity 5.33±1.56, p=0.01). The increase in tail moment and tail intensity returned to normal levels in both groups 2hours after the termination of ischemia.
  
  
  CONCLUSIONS
  Given that oxidative stress and genotoxic effect disappear in the late stages of reperfusion, we conclude that neither sevoflurane nor propofol can be considered superior to the other in anesthesia practices for extremity surgeries involving the use of a tourniquet.
authors.affiliation: Gazi University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Ankara, Turkey. Electronic address: serdenish@yahoo.com.
authors.forename: Sema
authors.initials: S
authors.lastname: Oncul
authors.affiliation: Gazi University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Ankara, Turkey.
authors.forename: Lale
authors.initials: L
authors.lastname: Karabiyik
authors.affiliation: Gazi University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Ankara, Turkey.
authors.forename: Erdem
authors.initials: E
authors.lastname: Coskun
authors.affiliation: Gazi University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Ankara, Turkey.
authors.forename: Ela
authors.initials: E
authors.lastname: Kadioglu
authors.affiliation: Gazi University Faculty of Medicine, Department of Anesthesiology and Intensive Care, Ankara, Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Gulbahar
mesh_terms: D000208:Acute Disease; D018685:Anesthetics, Inhalation / Q000494:pharmacology*; D018686:Anesthetics, Intravenous / Q000494:pharmacology*; D000818:Animals; D020552:Comet Assay; D004249:DNA Damage / Q000187:drug effects*; D008315:Malondialdehyde / Q000378:metabolism; D008738:Methyl Ethers / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D015742:Propofol / Q000494:pharmacology*; D011817:Rabbits; D011897:Random Allocation; D015427:Reperfusion Injury; D000077149:Sevoflurane; D014111:Tourniquets / Q000009:adverse effects
chemical_list: D018685:Anesthetics, Inhalation; D018686:Anesthetics, Intravenous; D008738:Methyl Ethers; D000077149:Sevoflurane; D008315:Malondialdehyde; D015742:Propofol
keywords: Estresse oxidativo; Genotoxicidade; Genotoxicity; Ischemia reperfusion injury; Lesão de isquemia reperfusão; Oxidative stress; Propofol; Sevoflurane; Sevoflurano