title: Deinococcus knuensis sp. nov., a bacterium isolated from river water.
abstract: Strain 16F3HT, a Gram-negative, aerobic, non-motile, and oval-shaped bacterium, was isolated from river water collected from the Han River in South Korea. Growth of strain 16F3HTwas observed at 10-42 °C (optimum at 25-30 °C), but no growth occurred at 4 °C. The strain is able to grow at pH 4-10 (optimum at pH 7-8) and tolerates up to 4% NaCl (w/v), with optimum growth at 0.5% NaCl. The isolate was found to be resistant to UV irradiation. Based on 16S rRNA gene sequence analysis, it is closely related to 'Deinococcus seoulensis' 16F1E (98.8%), Deinococcus aquaticus PB314T(98.1%) and Deinococcus caeni Ho-08T(98.0%). The level of DNA-DNA homology between the novel strain and the three related strains was 57.4, 41.2, and 35.8%, respectively. Chemotaxonomic data revealed that strain 16F3HTpossesses MK-8 as the predominant respiratory quinone, an unidentified phosphoglycolipid as the major polar lipid, and C15:1ω6c and C16:1ω7c as the major fatty acids. The DNA G + C content was determined to be 65.7 mol%. Based on polyphasic evidence, strain 16F3HT(=KCTC 33794T= JCM 31406T) should be classified as the type strain of a novel Deinococcus species, for which the name Deinococcus knuensis sp. nov. is proposed.
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Jae-Jin
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Yeon-Hee
authors.initials: YH
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Park
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Seung-Yeol
authors.initials: SY
authors.lastname: Lee
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Sangkyu
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Environmental Engineering, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Dae Sung
authors.initials: DS
authors.lastname: Lee
authors.affiliation: Department of Horticultural Science, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: In-Kyu
authors.initials: IK
authors.lastname: Kang
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea.
authors.forename: Leonid N
authors.initials: LN
authors.lastname: Ten
authors.affiliation: School of Applied Biosciences, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.|Institute of Plant Medicine, Kyungpook National University, Daegu, 41566, Republic of Korea. heeyoung@knu.ac.kr.
authors.forename: Hee-Young
authors.initials: HY
authors.lastname: Jung
mesh_terms: D016227:Benzoquinones / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D034301:Deinococcus / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification* / Q000528:radiation effects; D005227:Fatty Acids / Q000032:analysis; D005618:Fresh Water / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D010641:Phenotype; D010743:Phospholipids / Q000032:analysis; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D045483:Rivers / Q000382:microbiology*; D012965:Sodium Chloride / Q000378:metabolism; D013045:Species Specificity; D013696:Temperature; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000032:analysis
chemical_list: D016227:Benzoquinones; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C004532:quinone; D012965:Sodium Chloride; C024223:vitamin MK 8
keywords: 16S rRNA gene; Deinococcus; Polyphasic taxonomy; River water; UV resistance