title: Placental Proteomics Provides Insights into Pathophysiology of Pre-Eclampsia and Predicts Possible Markers in Plasma.
abstract: Pre-eclampsia is a hypertensive disorder characterized by the new onset of hypertension >140/90 mmHg and proteinuria after the 20th week of gestation. The disorder is multifactorial and originates with abnormal placentation. Comparison of the placental proteome of normotensive (n = 25) and pre-eclamptic (n = 25) patients by gel-free proteomic techniques identified a total of 2145 proteins in the placenta of which 180 were differentially expressed (>1.3 fold, p < 0.05). Gene ontology enrichment analysis of biological process suggested that the differentially expressed proteins belonged to various physiological processes such as angiogenesis, apoptosis, oxidative stress, hypoxia, and placental development, which are implicated in the pathophysiology of pre-eclampsia. Some of the differentially expressed proteins were monitored in the plasma by multiple reaction monitoring analysis, which showed an increase in apolipoproteins A-I and A-II in gestational weeks 26-30 (2-fold, p < 0.01), while haptoglobin and hemopexin decreased in gestational weeks 26-30 and week 40/at delivery (1.8 fold, p < 0.01) in pre-eclamptic patients. This study provides a proteomic insight into the pathophysiology of pre-eclampsia. Identified candidate proteins can be evaluated further for the development of potential biomarkers associated with pre-eclampsia pathogenesis.
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory , Homi Bhabha Road, Pune, Maharashtra 411008, India.
authors.forename: Sheon
authors.initials: S
authors.lastname: Mary
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory , Homi Bhabha Road, Pune, Maharashtra 411008, India.
authors.forename: Mahesh J
authors.identifier: 0000-0003-3932-9092
authors.initials: MJ
authors.lastname: Kulkarni
authors.affiliation: Sciex , 121 Udyog Vihar, Gurgaon, Haryana 122015, India.
authors.forename: Dipankar
authors.initials: D
authors.lastname: Malakar
authors.affiliation: Department of Nutritional Medicine, Interactive Research School for Health Affairs , Pune, Dhankawadi 411043, India.
authors.forename: Sadhana R
authors.initials: SR
authors.lastname: Joshi
authors.affiliation: Department of ObGy, Bharati Hospital , Pune, Maharashtra 411043, India.
authors.forename: Savita S
authors.initials: SS
authors.lastname: Mehendale
authors.affiliation: Division of Biochemical Sciences, CSIR-National Chemical Laboratory , Homi Bhabha Road, Pune, Maharashtra 411008, India.
authors.forename: Ashok P
authors.initials: AP
authors.lastname: Giri
mesh_terms: D000328:Adult; D016632:Apolipoprotein A-I / Q000097:blood / Q000235:genetics; D016633:Apolipoprotein A-II / Q000097:blood / Q000235:genetics; D017209:Apoptosis / Q000235:genetics; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D005865:Gestational Age; D006242:Haptoglobins / Q000235:genetics / Q000378:metabolism; D006466:Hemopexin / Q000235:genetics / Q000378:metabolism; D006801:Humans; D000860:Hypoxia / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D058977:Molecular Sequence Annotation; D009389:Neovascularization, Pathologic / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D018384:Oxidative Stress; D010920:Placenta / Q000098:blood supply / Q000378:metabolism* / Q000473:pathology; D011225:Pre-Eclampsia / Q000097:blood* / Q000175:diagnosis / Q000235:genetics / Q000473:pathology; D011247:Pregnancy; D025941:Protein Interaction Mapping; D020543:Proteome / Q000235:genetics* / Q000378:metabolism; D040901:Proteomics / Q000379:methods*
chemical_list: D016632:Apolipoprotein A-I; D016633:Apolipoprotein A-II; D015415:Biomarkers; D006242:Haptoglobins; D020543:Proteome; D006466:Hemopexin
keywords: hypertension; label-free proteomics; multiple reaction monitoring; placenta; pregnancy