title: β-Amyrin synthase (EsBAS) and β-amyrin 28-oxidase (CYP716A244) in oleanane-type triterpene saponin biosynthesis in Eleutherococcus senticosus.
abstract: Siberian ginseng (Eleutherococcus senticosus) is a woody medical shrub belonging to the Araliaceae family. E. senticosus contains various types of saponins, including oleanane, noroleanane, lupane, and 3,4-secolupane types, depending on the aglycone structure. Oleanane-type triterpenes are the major saponin components in E. senticosus. Two enzymes (β-amyrin synthase and β-amyrin 28-oxidase) are essential for oleanane-type saponin biosynthesis from 2,3-oxidosqualene. In the present study, two full-length cDNAs encoding EsBAS and CYP716A244 were isolated based on transcriptomics analysis of plant leaves. Both β-amyrin synthase (EsBAS) and β-amyrin 28-oxidase (CYP716A244), isolated from E. senticosus, were functionally characterised. β-amyrin production was confirmed by heterologous expression of the EsBAS gene in yeast and tobacco. Oleanolic acid production was confirmed by co-expression of both EsBAS and CYP716A244 in engineered yeast and transgenic tobacco.
authors.affiliation: Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Hye-Jeong
authors.initials: HJ
authors.lastname: Jo
authors.affiliation: Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Jung Yeon
authors.initials: JY
authors.lastname: Han
authors.affiliation: Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea.
authors.forename: Hwan-Su
authors.initials: HS
authors.lastname: Hwang
authors.affiliation: Department of Forest Resources, College of Forest and Environmental Sciences, Kangwon National University, Chuncheon 200-701, Republic of Korea. Electronic address: yechoi@kangwon.ac.kr.
authors.forename: Yong Eui
authors.initials: YE
authors.lastname: Choi
mesh_terms: D000595:Amino Acid Sequence; D003577:Cytochrome P-450 Enzyme System / Q000378:metabolism*; D018076:DNA, Complementary / Q000378:metabolism; D028001:Eleutherococcus / Q000737:chemistry* / Q000201:enzymology; D019751:Intramolecular Transferases / Q000378:metabolism*; D015394:Molecular Structure; D009828:Oleanolic Acid / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D005894:Panax / Q000235:genetics; D010946:Plants, Medicinal / Q000737:chemistry; D012503:Saponins / Q000378:metabolism; D013185:Squalene / Q000031:analogs & derivatives / Q000378:metabolism; D014315:Triterpenes / Q000737:chemistry* / Q000378:metabolism; D053978:Pentacyclic Triterpenes
chemical_list: D003577:Cytochrome P-450 Enzyme System; D018076:DNA, Complementary; D019751:Intramolecular Transferases; D009828:Oleanolic Acid; D010088:Oxidoreductases; D012503:Saponins; D013185:Squalene; D014315:Triterpenes; C520393:oxidosqualene; C480546:lupane; D053978:Pentacyclic Triterpenes; C039924:2,3-oxidosqualene-beta-amyrin-cyclase
keywords: Araliaceae; Eleutherococcus senticosus; Oleanolic acid synthase; Saponin biosynthesis; β-amyrin synthase