title: MicroRNA-335-5p and -3p synergize to inhibit estrogen receptor alpha expression and promote tamoxifen resistance.
abstract: microRNAs (miRNAs) are small noncoding RNA molecules involved in the regulation of gene expression and play critical roles in human malignancies. Next-generation sequencing analysis of the MCF-7 breast cancer cell line overexpressing miR-335-5p and miR-335-3p demonstrated that the miRNA duplex repressed genes involved in the ERα signaling pathway, and enhanced resistance of MCF-7 cells to the growth inhibitory effects of tamoxifen. These data suggest that despite its conventional role in tumor suppression, the miR-335 transcript can also play an oncogenic role in promoting agonistic estrogen signaling in a cancerous setting.
authors.affiliation: Department of Biological and Agricultural Engineering, Louisiana State University and LSU Agricultural Center, Baton Rouge, LA, USA.
authors.forename: Elizabeth C
authors.initials: EC
authors.lastname: Martin
authors.affiliation: Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, USA.
authors.forename: Adrienne K
authors.initials: AK
authors.lastname: Conger
authors.affiliation: Department of Medicine-Section of Hematology and Medical Oncology, Tulane University, New Orleans, LA, USA.
authors.forename: Thomas J
authors.initials: TJ
authors.lastname: Yan
authors.affiliation: Department of Medicine-Section of Hematology and Medical Oncology, Tulane University, New Orleans, LA, USA.
authors.forename: Van T
authors.initials: VT
authors.lastname: Hoang
authors.affiliation: Medical Sciences and Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Bloomington, IN, USA.
authors.forename: David F B
authors.initials: DF
authors.lastname: Miller
authors.affiliation: Indiana University Center for Genomics and Bioinformatics, Bloomington, IN, USA.
authors.forename: Aaron
authors.initials: A
authors.lastname: Buechlein
authors.affiliation: Indiana University Center for Genomics and Bioinformatics, Bloomington, IN, USA.
authors.forename: Douglas B
authors.initials: DB
authors.lastname: Rusch
authors.affiliation: Medical Sciences and Department of Cellular and Integrative Physiology, Indiana University School of Medicine, Bloomington, IN, USA.
authors.forename: Kenneth P
authors.initials: KP
authors.lastname: Nephew
authors.affiliation: Department of Medicine-Section of Hematology and Medical Oncology, Tulane University, New Orleans, LA, USA.
authors.forename: Bridgette M
authors.initials: BM
authors.lastname: Collins-Burow
authors.affiliation: Department of Medicine-Section of Hematology and Medical Oncology, Tulane University, New Orleans, LA, USA.|Department of Pharmacology, Tulane University, New Orleans, LA, USA.|Tulane Cancer Center, Tulane University, New Orleans, LA, USA.
authors.forename: Matthew E
authors.initials: ME
authors.lastname: Burow
mesh_terms: D001943:Breast Neoplasms / Q000235:genetics; D019008:Drug Resistance, Neoplasm / Q000187:drug effects / Q000235:genetics*; D047628:Estrogen Receptor alpha / Q000235:genetics* / Q000378:metabolism; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D020935:MAP Kinase Signaling System / Q000187:drug effects; D061986:MCF-7 Cells; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013629:Tamoxifen / Q000494:pharmacology*
chemical_list: C506487:ESR1 protein, human; D047628:Estrogen Receptor alpha; C556347:MIRN335 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; D013629:Tamoxifen
keywords: breast cancer; endocrine resistance; estrogen receptor; isoform; miRNA-335; microRNA