title: Regulation of BC200 RNA-mediated translation inhibition by hnRNP E1 and E2.
abstract: The long noncoding RNA BC200 (brain cytoplasmic RNA, 200 nucleotides) acts as a translational modulator of local protein synthesis at dendrites. BC200 RNA has been shown to inhibit translation in vitro, but it remains unknown how this translation inhibition might be controlled in a cell. Here, we performed yeast three-hybrid screening and identified hnRNP E1 and hnRNP E2 as BC200 RNA-interacting proteins. We found that: these hnRNA proteins could restore BC200 RNA-inhibited translation; BC200 RNA interacts with hnRNP E1 and E2 mainly through its unique 3' C-rich domain; and the RNA binding specificities and modes of the two proteins differed somewhat. Our results offer new insights into the regulation of BC200 RNA-mediated translation inhibition.
authors.affiliation: Department of Chemistry, KAIST, Daejeon, Korea.
authors.forename: Seonghui
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Chemistry, KAIST, Daejeon, Korea.
authors.forename: Heegwon
authors.initials: H
authors.lastname: Shin
authors.affiliation: Department of Chemistry, KAIST, Daejeon, Korea.
authors.forename: Jungmin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Chemistry, KAIST, Daejeon, Korea.
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Chemistry, KAIST, Daejeon, Korea.
authors.forename: Geunu
authors.initials: G
authors.lastname: Bak
authors.affiliation: Department of Chemistry, KAIST, Daejeon, Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Lee
mesh_terms: D001483:Base Sequence; D004268:DNA-Binding Proteins; D039601:Eukaryotic Initiation Factor-4A / Q000378:metabolism; D006367:HeLa Cells; D034441:Heterogeneous-Nuclear Ribonucleoproteins / Q000378:metabolism*; D006801:Humans; D009690:Nucleic Acid Conformation; D011485:Protein Binding; D014176:Protein Biosynthesis; D062085:RNA, Long Noncoding / Q000378:metabolism*; D016601:RNA-Binding Proteins / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D034441:Heterogeneous-Nuclear Ribonucleoproteins; C094604:PCBP1 protein, human; C501903:PCBP2 protein, human; D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; C000609640:long non-coding RNA BC200, human; D039601:Eukaryotic Initiation Factor-4A
keywords: BC200 RNA; RNA-protein interaction; hnRNP E1; hnRNP E2; regulation of translation