title: An improved approach for evaluating the semicrystalline lamellae of starch granules by synchrotron SAXS.
abstract: A fitting method combined with a linear correlation function was developed as an improved approach for the SAXS analysis of the semicrystalline lamellae of starch granules. Using a power-law function with two Gaussian plus Lorentz functions, the SAXS pattern was resolved into sub-patterns of the net lamellar peak and the power-law scattering plus scattering background (PL+B). The ratio of the net lamellar peak area (Apeak) to the total scattering area (Atotal) was proposed equal to the proportion of the lamellae within the starch granule (PSL). Along with this fitting method, we obtained a better profile of linear correlation function, with the elimination of the interference of non-lamellar amorphous starch (i.e., amorphous growth rings). Then, we could accurately calculate the lamellar parameters, e.g., PSL, the thicknesses of semicrystalline (d), crystalline (dc) and amorphous (da) lamellae, and the volume fraction (φc) of crystalline lamellae within semicrystalline lamellae. Quantitative analysis revealed that PSLwas positively correlated with the crystallinity (Xc) of starch. It was confirmed that the distribution of lamellar thickness was more important than the starch botanical origin in affecting the validity of the developed fitting method. We also proposed a criterion to test the validity of the proposed method. Specifically, the total SAXS pattern should be mostly tangent to the profile of PL+B at a high q tail (close to 0.2Å-1).
authors.affiliation: Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
authors.forename: Binjia
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Chemical Engineering, The University of Queensland, Brisbane, Qld 4072, Australia.
authors.forename: Fengwei
authors.initials: F
authors.lastname: Xie
authors.affiliation: School of Chemical Engineering, The University of Queensland, Brisbane, Qld 4072, Australia.
authors.forename: David K
authors.initials: DK
authors.lastname: Wang
authors.affiliation: Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Siming
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Niu
authors.affiliation: Glyn O. Philips Hydrocolloid Research Centre at HUT, Hubei University of Technology, Wuhan 430068, China. Electronic address: qdttkl@163.com.
authors.forename: Dongling
authors.initials: D
authors.lastname: Qiao
authors.affiliation: Laboratory of Environment Correlative Dietology (Ministry of Education), College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
authors.forename: Shanbai
authors.initials: S
authors.lastname: Xiong
authors.affiliation: Glyn O. Philips Hydrocolloid Research Centre at HUT, Hubei University of Technology, Wuhan 430068, China.
authors.forename: Fatang
authors.initials: F
authors.lastname: Jiang
authors.affiliation: College of Chemistry and Environmental Engineering, Dongguan University of Technology, Dongguan 523808, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, South China University of Technology, Guangzhou 510640, China.
authors.forename: Long
authors.initials: L
authors.lastname: Yu
mesh_terms: D053838:Scattering, Small Angle; D013213:Starch / Q000737:chemistry*; D017356:Synchrotrons; D014961:X-Ray Diffraction
chemical_list: D013213:Starch
keywords: Granule; Methodology; Semicrystalline lamellae; Starch; Starch (PubChem CID: 24836924); Water (PubChem CID: 962); X-ray scattering