title: Preliminary study on the corrosion resistance, antibacterial activity and cytotoxicity of selective-laser-melted Ti6Al4V-xCu alloys.
abstract: In this study, a series of Cu-bearing Ti6Al4V-xCu (x=0, 2, 4, 6wt%) alloys (shorten by Ti6Al4V, 2C, 4C, and 6C, respectively.) with antibacterial function were successfully fabricated by selective laser melting (SLM) technology with mixed spherical powders of Cu and Ti6Al4V for the first time. In order to systematically investigate the effects of Cu content on the microstructure, phase constitution, corrosion resistance, antibacterial properties and cytotoxicity of SLMed Ti6Al4V-xCu alloys, experiments including XRD, SEM-EDS, electrochemical measurements, antibacterial tests and cytotoxicity tests were conducted with comparison to SLMed Ti6Al4V alloy (Ti6Al4V). Microstructural observations revealed that Cu had completely fused into the Ti6Al4V alloy, and presented in the form of Ti2Cu phase at ambient temperature. With Cu content increase, the density of the alloy gradually decreased, and micropores were obviously found in the alloy. Electrochemical measurements showed that corrosion resistance of Cu-bearing alloys were stronger than Cu-free alloy. Antibacterial tests demonstrated that 4C and 6C alloys presented strong and stable antibacterial property against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) compared to the Ti6Al4V and 2C alloy. In addition, similar to the Ti6Al4V alloy, the Cu-bearing alloys also exerted good cytocompatibility to the Bone Marrow Stromal Cells (BMSCs) from Sprague Dawley (SD) rats. Based on those results, the preliminary study verified that it was feasible to fabricated antibacterial Ti6Al4V-xCu alloys direct by SLM processing mixed commercial Ti6Al4V and Cu powder.
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Sai
authors.initials: S
authors.lastname: Guo
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China.
authors.forename: Yanjin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China.
authors.forename: Songquan
authors.initials: S
authors.lastname: Wu
authors.affiliation: Key Laboratory of Stomatology (Fujian Medical University), Fujian Province University, Fuzhou, Fujian, China.
authors.forename: Lingling
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Stomatology (Fujian Medical University), Fujian Province University, Fuzhou, Fujian, China.
authors.forename: Mengjiao
authors.initials: M
authors.lastname: He
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China.
authors.forename: Chaoqian
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China.
authors.forename: Yiliang
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Lin
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jiasi
authors.initials: J
authors.lastname: Luo
authors.affiliation: Key Laboratory of Stomatology (Fujian Medical University), Fujian Province University, Fuzhou, Fujian, China.
authors.forename: Xiongcheng
authors.initials: X
authors.lastname: Xu
authors.affiliation: Key Laboratory of Optoelectronic Materials Chemistry and Physics, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: franklin@fjirsm.ac.cn.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Lin
mesh_terms: D000497:Alloys; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D001854:Bone Marrow Cells / Q000166:cytology; D002470:Cell Survival / Q000187:drug effects; D003300:Copper / Q000737:chemistry; D003343:Corrosion; D058266:Dielectric Spectroscopy; D004926:Escherichia coli / Q000187:drug effects; D007834:Lasers; D051381:Rats; D017207:Rats, Sprague-Dawley; D013211:Staphylococcus aureus / Q000187:drug effects; D017154:Stromal Cells / Q000166:cytology / Q000187:drug effects / Q000378:metabolism; D014025:Titanium / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D014961:X-Ray Diffraction
chemical_list: D000497:Alloys; D000900:Anti-Bacterial Agents; C031462:titanium alloy (TiAl6V4); D003300:Copper; D014025:Titanium
keywords: Antibacterial property; Corrosion resistance; Cytotoxicity; Selective laser melting; Titanium alloy