title: [Mechanism of Regulating BAFF Signaling Pathway by Act1 in B Cell Lymphoma].
abstract: OBJECTIVE
  To explore the mechanism of regulating B cell-activating factor (BAFF) signalling pathway by NF-κB activator 1 (Act1) in B cell lymphoma so as to provide a new thinking for treatment of B cell lymphoma.
  
  
  METHODS
  The human B cell lymphoma cell lines including Raji, Daudi and BALL-1 were cultured, when the cells were in logarithmic phase, the RNA was extracted, and the Act1 was amplified by RT-PCR; and pTT5-Act1 expression plasmid was constructed and was transfected into cells; the Act1 was silenced by using Act1 mRNA; the NF-κB signaling pathway protein was detected by Western blot.
  
  
  RESULTS
  After silence or overexpression of Act1, the proliferation levels of Raji, Daudi and BALL-1 cells were up- or down-regulated, respectively. Overexpression and silence of Act1 could down-or up-regulate BAFF-R expression level, furthermore could inhibit or activate of NF-κB signalling pahway, respectively.
  
  
  CONCLUSIONS
  Among 3 above-mentioned B cell lymphoma cell lines, Act1 plays negative regulating role, indicating that the Act1 may be a promising therapeutic target for treating B cell lymphoma.
authors.affiliation: Department of Laboratorial Examination, Affliated Hospital of Zun Yi Medical College, Zunyi, 563000, Guizhou Province, China.
authors.forename: Yong-Lun
authors.initials: YL
authors.lastname: Wang
authors.affiliation: Department of Laboratorial Examination, Affliated Hospital of Zun Yi Medical College, Zunyi, 563000, Guizhou Province, China.
authors.forename: Xun
authors.initials: X
authors.lastname: Min
authors.affiliation: Department of Laboratorial Examination, Affliated Hospital of Zun Yi Medical College, Zunyi, 563000, Guizhou Province, China. E-mail:gxj_199421@163.com.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Ge
mesh_terms: D053264:B-Cell Activating Factor; D053265:B-Cell Activation Factor Receptor; D006801:Humans; D016393:Lymphoma, B-Cell; D016328:NF-kappa B; D012333:RNA, Messenger; D015398:Signal Transduction; D015854:Up-Regulation
chemical_list: D053264:B-Cell Activating Factor; D053265:B-Cell Activation Factor Receptor; D016328:NF-kappa B; D012333:RNA, Messenger; C435926:TNFRSF13C protein, human; C505719:TNFSF13B protein, human