title: Small GTPase Arl6 controls RH30 rhabdomyosarcoma cell growth through ciliogenesis and Hedgehog signaling.
abstract: BACKGROUND
  Rhabdomyosarcoma (RMS) originates from skeletal muscle precursors that fail to differentiate. Hedgehog (Hh) signaling and primary cilia contribute to the pathobiology of RMS.
  
  
  RESULTS
  Here we showed ADP ribosylation factor like GTPase 6 (ARL6) localizes at the base of primary cilium, controls ciliogenesis and Hh signaling. The transcription of Arl6 is dynamic during the differentiation of myoblasts, companying with the growth and elimination of primary cilia. Arl6 expression is significantly up regulated in cilia-dependent RMS cells and tissues. Knockdown of Arl6 inhibits proliferation and promotes apoptosis of RMS RH30 cells through defected ciliogenesis and reduced Hh activity.
  
  
  CONCLUSIONS
  Taken together, the functions of Arl6 in ciliogenesis and Hh signaling suggest it as a potential RMS drug target.
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Shen
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Yu
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Ziyu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ding
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.|Central Laboratory, The First People's Hospital of Wujiang District, Suzhou, 215200 People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Ning
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Developmental Genetics, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, 211166 Jiangsu People's Republic of China.
authors.forename: Shen
authors.initials: S
authors.lastname: Yue
keywords: Hedgehog; Primary cilia; Rhabdomyosarcoma