title: Association of the polymorphism 12109g>A from the REN gene as a risk factor for preterm birth.
abstract: BACKGROUND
  Preterm birth is the most important cause of neonatal mortality and morbidity. It is a multifactorial disease with different etiologies, including genetic factors. Genetic variability is represented by single nucleotide polymorphisms (SNPs) in genes of proteins involved in the contractile activity. We determine the association between SNP 12109G> A in REN associated with preterm birth and premature rupture of membrane.
  
  
  METHODS
  A study of cases ( N=112, 22-36 weeks of gestation; mean: 31, 95% confidence interval 30.7-32.2) and controls ( N=66; 38-40 weeks of gestation from the last menstrual period; mean: 39.8, 95% confidence interval 38.9-39.4) was performed. Genomic DNA was isolated in all patients from peripheral blood. The SNP 12109G> A ( Mbo I) in REN was typified by PCR-restriction fragment length polymorphism.
  
  
  RESULTS
  A significant difference in the case group for the SNP 12109G>A was observed. The A allele was increased in women with preterm birth (81% cases vs. 15% control, p<0.0000004). There was also a significant difference between genotypes, mainly an excess of G/A heterozygotes in women with preterm birth (60% cases vs. 23% controls). The phenotype 12109G> A has odds ratio 6.62 (95% confidence interval 3.14-14.15), which means a high risk of preterm birth/premature rupture of membrane in presence of allele A, both in homozygotes and in heterozygotes.
  
  
  CONCLUSIONS
  Allelic frequency of A of SNP 12109G>A was higher in women with preterm birth than in women with normal vaginal delivery and could be considered a risk factor.
authors.affiliation: 1 Genetics Department, 'Dr. José Eleuterio González' University Hospital, Universidad Autonoma de Nuevo León, Monterrey, Mexico.
authors.forename: Irám P
authors.initials: IP
authors.lastname: Rodríguez-Sánchez
authors.affiliation: 2 Faculty of Chemical Sciences, Universidad de Colima, Coquimatlán, Mexico.
authors.forename: Stephania
authors.initials: S
authors.lastname: Suárez-Caro
authors.affiliation: 3 Western General Hospital, Health Secretary of Jalisco, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Rivas-Solís
authors.affiliation: 4 Faculty of Medicine, Universidad de Colima, Mexico.|5 State Cancer Institute, Health Secretary de Colima, Mexico.
authors.forename: Iván
authors.initials: I
authors.lastname: Delgado-Enciso
authors.affiliation: 6 Developmental Biology Unit, Laboratory of Immunology and Virology, Faculty of Biological Sciences, Universidad Autónoma de Nuevo León, San Nicolas de los Garza, Mexico.
authors.forename: María M
authors.initials: MM
authors.lastname: Sánchez-Chaparro
authors.affiliation: 7 Faculty of Biological Sciences, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
authors.forename: Mayra A
authors.initials: MA
authors.lastname: Gómez-Govea
authors.affiliation: 1 Genetics Department, 'Dr. José Eleuterio González' University Hospital, Universidad Autonoma de Nuevo León, Monterrey, Mexico.
authors.forename: Laura E
authors.initials: LE
authors.lastname: Martínez-de-Villarreal
authors.affiliation: 2 Faculty of Chemical Sciences, Universidad de Colima, Coquimatlán, Mexico.|4 Faculty of Medicine, Universidad de Colima, Mexico.
authors.forename: Laura L
authors.initials: LL
authors.lastname: Valdez-Velazquez
mesh_terms: D000483:Alleles; D016022:Case-Control Studies; D004591:Electrophoresis, Polyacrylamide Gel; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006801:Humans; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D047928:Premature Birth / Q000235:genetics*; D012083:Renin / Q000235:genetics*; D012307:Risk Factors
chemical_list: D012083:Renin
keywords: Mbo I; PCR-RFLP; Preterm birth; REN gene; SNPs