title: Mycobacterium bovis Requires P27 (LprG) To Arrest Phagosome Maturation and Replicate within Bovine Macrophages.
abstract: Mycobacterium boviscauses tuberculosis in a wide variety of mammals, with strong tropism for cattle and eventually humans. P27, also called LprG, is among the proteins involved in the mechanisms of the virulence and persistence ofM. bovisandMycobacterium tuberculosisHere, we describe a novel function of P27 in the interaction ofM. boviswith its natural host cell, the bovine macrophage. We found that a deletion in thep27-p55operon impairs the replication ofM. bovisin bovine macrophages. Importantly, we show for the first time thatM. bovisarrests phagosome maturation in a process that depends on P27. This effect is P27 specific since complementation with wild-typep27but notp55fully restored the wild-type phenotype of the mutant strain; this indicates that P55 plays no important role during the early events ofM. bovisinfection. In addition, we also showed that the presence of P27 fromM. smegmatisdecreases the association of LAMP-3 with bead phagosomes, indicating that P27 itself blocks phagosome-lysosome fusion by modulating the traffic machinery in the cell host.
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Nicolás Repetto y De Los Reseros, Buenos Aires, Argentina.
authors.forename: Cristina Lourdes
authors.initials: CL
authors.lastname: Vázquez
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Nicolás Repetto y De Los Reseros, Buenos Aires, Argentina.
authors.forename: María Verónica
authors.initials: MV
authors.lastname: Bianco
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Nicolás Repetto y De Los Reseros, Buenos Aires, Argentina.
authors.forename: Federico Carlos
authors.initials: FC
authors.lastname: Blanco
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Nicolás Repetto y De Los Reseros, Buenos Aires, Argentina.
authors.forename: Marina Andrea
authors.initials: MA
authors.lastname: Forrellad
authors.affiliation: Host-Pathogen Interactions in Tuberculosis Laboratory, The Francis Crick Institute, London, United Kingdom.
authors.forename: Maximiliano Gabriel
authors.initials: MG
authors.lastname: Gutierrez
authors.affiliation: Instituto de Biotecnología, CICVyA-INTA, Nicolás Repetto y De Los Reseros, Buenos Aires, Argentina bigi.fabiana@inta.gob.ar.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Bigi
mesh_terms: D000818:Animals; D002417:Cattle; D059447:Cell Cycle Checkpoints; D015870:Gene Expression; D006367:HeLa Cells; D006801:Humans; D008074:Lipoproteins / Q000235:genetics / Q000378:metabolism*; D008264:Macrophages / Q000382:microbiology* / Q000502:physiology*; D050296:Microbial Viability; D009154:Mutation; D009163:Mycobacterium bovis / Q000502:physiology*; D052247:Nitric Oxide Synthase Type II / Q000235:genetics / Q000378:metabolism; D009876:Operon; D010588:Phagosomes / Q000378:metabolism* / Q000382:microbiology*
chemical_list: D008074:Lipoproteins; C110121:P27 protein, Mycobacterium; D052247:Nitric Oxide Synthase Type II
keywords: LprG; Mycobacterium bovis; P27