title: Roles of afadin in the formation of the cellular architecture of the mouse hippocampus and dentate gyrus.
abstract: The hippocampal formation with tightly packed neurons, mainly at the dentate gyrus, CA3, CA2, and CA1 regions, constitutes a one-way neural circuit, which is associated with learning and memory. We previously showed that the cell adhesion molecules nectins and its binding protein afadin play roles in the formation of the mossy fiber synapses which are formed between the mossy fibers of the dentate gyrus granule cells and the dendrites of the CA3 pyramidal cells. We showed here that in the afadin-deficient hippocampal formation, the dentate gyrus granules cells and the CA3, CA2, and CA1 pyramidal cells were abnormally located; the mossy fiber trajectory was abnormally elongated; the CA3 pyramidal cells were abnormally differentiated; and the densities of the presynaptic boutons on the mossy fibers and the apical dendrites of the CA3 pyramidal cells were decreased. These results indicate that afadin plays roles not only in the formation of the mossy fiber synapses but also in the formation of the cellular architecture of the hippocampus and the dentate gyrus.
authors.affiliation: Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Muneaki
authors.initials: M
authors.lastname: Miyata
authors.affiliation: Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan; CREST, Japan Science and Technology Agency, Kobe, Japan.
authors.forename: Tomohiko
authors.initials: T
authors.lastname: Maruo
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan; Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Aika
authors.initials: A
authors.lastname: Kaito
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan; Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Shujie
authors.initials: S
authors.lastname: Wang
authors.affiliation: Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Yamamoto
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan; Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Fujiwara
authors.affiliation: CREST, Japan Science and Technology Agency, Kobe, Japan; Department of Neural Regeneration and Cell Communication, Mie University Graduate School of Medicine, Tsu, Mie, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Mizoguchi
authors.affiliation: Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan; CREST, Japan Science and Technology Agency, Kobe, Japan. Electronic address: mandai@med.kobe-u.ac.jp.
authors.forename: Kenji
authors.initials: K
authors.lastname: Mandai
authors.affiliation: Division of Pathogenetic Signaling, Department of Biochemistry and Molecular Biology, Kobe University Graduate School of Medicine, Kobe, Japan; CREST, Japan Science and Technology Agency, Kobe, Japan. Electronic address: ytakai@med.kobe-u.ac.jp.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Takai
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D002478:Cells, Cultured; D018891:Dentate Gyrus / Q000166:cytology* / Q000254:growth & development / Q000378:metabolism; D051379:Mice; D008840:Microfilament Proteins / Q000235:genetics / Q000378:metabolism*; D019599:Mossy Fibers, Hippocampal / Q000378:metabolism; D055495:Neurogenesis; D017966:Pyramidal Cells / Q000166:cytology* / Q000378:metabolism; D013569:Synapses / Q000378:metabolism
chemical_list: D008840:Microfilament Proteins; C108835:afadin
keywords: Afadin; Cellular architecture; Dentate gyrus; Hippocampus; Mossy fiber; Pyramidal cells