title: Ionic current modulation from DNA translocation through nanopores under high ionic strength and concentration gradients.
abstract: Ion transport through nanopores is an important process in nature and has important engineering applications. To date, most studies of nanopore ion transport have been carried out with electrolytes of relatively low concentrations. In this paper, we report on ionic current modulation from the translocation of dsDNA through a nanopore under high ionic strength and with an electrolyte concentration gradient across the nanopore. Results show that in this case, DNA translocation can induce either negative or positive ionic current modulation, even though usually only downward peaks are expected under this high ion concentration. Through a series of experiments and numerical simulations with nanopores of different diameters and concentration gradients, it is found that the positive pulse is due to extra ions outside the electric double layer of the DNA that are brought into the nanopore by the enhanced electroosmotic flow (EOF) with the negatively charged DNA inside the nanopore.
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn.
authors.forename: Yin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn.
authors.forename: Gensheng
authors.initials: G
authors.lastname: Wu
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Si
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn.
authors.forename: Jian
authors.initials: J
authors.lastname: Ma
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn.
authors.forename: Zhishan
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: China Education Council Key Laboratory of MEMS, Southeast University, Nanjing 210096, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Xie
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn.
authors.forename: Jingjie
authors.initials: J
authors.lastname: Sha
authors.affiliation: Department of Mechanical Engineering, Vanderbilt University, Nashville, TN 37235-1592, USA.
authors.forename: Deyu
authors.initials: D
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory for Design and Fabrication of Micro-Nano Biomedical Instruments, School of Mechanical Engineering, Southeast University, Nanjing 211189, China. yunfeichen@seu.edu.cn and State Key Laboratory of Bioelectronics, Southeast University, Nanjing 211189, China.
authors.forename: Yunfei
authors.initials: Y
authors.lastname: Chen
mesh_terms: D004247:DNA / Q000737:chemistry*; D017136:Ion Transport; D007477:Ions; D058608:Nanopores; D009994:Osmolar Concentration
chemical_list: D007477:Ions; D004247:DNA