title: A conserved region in the Closterovirus 1a polyprotein drives extensive remodeling of endoplasmic reticulum membranes and induces motile globules in Nicotiana benthamiana cells.
abstract: In infected plant cells, closterovirus replicative polyproteins 1a and 1ab drive membrane remodeling and formation of multivesicular replication platforms. Polyprotein 1a contains a variable Central Region (CR) between the methyltransferase and helicase domains. In a previous study, we have found that transient expression of the Beet yellows virus CR-2 segment (aa 1305-1494) in Nicotiana benthamiana induces the formation of ~1µm mobile globules originating from the ER membranes. In the present study, sequence analysis has shown that a part of the CR named the "Zemlya region" (overlapping the CR-2), is conserved in all members of the Closterovirus genus and contains a predicted amphipathic helix (aa 1368-1385). By deletion analysis, the CR-2 region responsible for the induction of 1-μm globules has been mapped to aa 1368-1432. We suggest that the conserved membrane-modifying region of the BYV 1a may be involved in the biogenesis of closterovirus replication platforms.
authors.affiliation: Faculty of Biology, Moscow State University, Moscow 119991, Russia; N.F. Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Gushchin
authors.affiliation: 25, rue de Cassis, 13008 Marseille, France.
authors.forename: D G
authors.initials: DG
authors.lastname: Karlin
authors.affiliation: Faculty of Biology, Moscow State University, Moscow 119991, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Makhotenko
authors.affiliation: Faculty of Biology, Moscow State University, Moscow 119991, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Khromov
authors.affiliation: M.M. Shemyakin and Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, Russia.
authors.forename: T N
authors.initials: TN
authors.lastname: Erokhina
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow 119991, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Solovyev
authors.affiliation: A.N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia.
authors.forename: S Yu
authors.initials: SY
authors.lastname: Morozov
authors.affiliation: Faculty of Biology, Moscow State University, Moscow 119991, Russia; Center of Bioengineering, Russian Academy of Sciences, Moscow, Russia. Electronic address: aaa@genebee.msu.su.
authors.forename: A A
authors.initials: AA
authors.lastname: Agranovsky
mesh_terms: D000595:Amino Acid Sequence; D017790:Closterovirus / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D017124:Conserved Sequence; D004721:Endoplasmic Reticulum / Q000378:metabolism / Q000821:virology*; D008969:Molecular Sequence Data; D010935:Plant Diseases / Q000821:virology*; D020815:Polyproteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D016415:Sequence Alignment; D014026:Nicotiana / Q000821:virology*; D014764:Viral Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*
chemical_list: D020815:Polyproteins; D014764:Viral Proteins
keywords: Endoplasmic reticulum modification; Membrane vesicles; RNA virus replication; Virus replication factory