title: Bovine in vitro embryo production: the effects of fibroblast growth factor 10 (FGF10).
abstract: OBJECTIVE
  In an attempt to improve in vitro embryo production, we investigated the effect of fibroblast growth factor 10 (FGF10) during in vitro maturation on the developmental capacity of bovine oocytes.
  
  
  METHODS
  Cumulus-oocyte complexes (COCs) were aspirated from follicles of 3-8 mm diameter. After selection, the COCs were matured in medium with or without 0.5 ng/mL of FGF10. The effect of FGF10 during in vitro maturation (IVM) on nuclear maturation kinetics and expansion of the cumulus cells was investigated. Oocyte competence was assessed by the production and development speed of embryos and the relative expression of genes associated with embryo quality.
  
  
  RESULTS
  FGF10 delayed the resumption of meiosis from 8 h onwards, but did not affect the percentage of oocytes reaching metaphase II, nor did it increase cumulus expansion at 22 h of maturation. We found no difference between treatments regarding embryo production, developmental speed, and gene expression.
  
  
  CONCLUSIONS
  In conclusion, the presence of FGF10 during IVM had no effect on embryo production, developmental speed, and gene expression.
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.
authors.forename: Mateus Nunes
authors.initials: MN
authors.lastname: Diógenes
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.|Embrapa- Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Parque Estação Biológica, Final Av. W5/N, Prédio, PBI70770-900, Brasília, DF, Brazil.
authors.forename: Ana Luiza Silva
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil.|Embrapa- Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Parque Estação Biológica, Final Av. W5/N, Prédio, PBI70770-900, Brasília, DF, Brazil.
authors.forename: Ligiane Oliveira
authors.initials: LO
authors.lastname: Leme
authors.affiliation: School of Agriculture and Veterinary Medicine, University of Brasilia, Brasília, DF, Brazil. margot.dode@embrapa.br.|Embrapa- Genetic Resources and Biotechnology, Laboratory of Animal Reproduction, Parque Estação Biológica, Final Av. W5/N, Prédio, PBI70770-900, Brasília, DF, Brazil. margot.dode@embrapa.br.
authors.forename: Margot Alves Nunes
authors.initials: MA
authors.lastname: Dode
mesh_terms: D000818:Animals; D001755:Blastocyst / Q000378:metabolism; D002417:Cattle; D054885:Cumulus Cells / Q000378:metabolism; D046149:Embryo Culture Techniques; D047108:Embryonic Development / Q000235:genetics*; D005260:Female; D051526:Fibroblast Growth Factor 10 / Q000096:biosynthesis*; D018507:Gene Expression Regulation, Developmental; D059471:In Vitro Oocyte Maturation Techniques; D008540:Meiosis / Q000235:genetics; D009865:Oocytes / Q000254:growth & development* / Q000378:metabolism
chemical_list: D051526:Fibroblast Growth Factor 10
keywords: Blastocyst; Cattle; Cumulus expansion; Maturation; Oocyte