title: Effects of Fe-deficient conditions on Fe uptake and utilization in P-efficient soybean.
abstract: Phosphorus (P)-efficient soybean (Glycine max) plants absorb and utilize P with high efficiency. To investigate the effects of iron (Fe)-deficient conditions on the absorption and utilization of Fe in P-efficient soybean plants, two soybean cultivars with different P efficiency, the 03-3 (P-efficient variety) and Bd-2 (P-inefficient variety), were used in this study. The two soybean cultivars were grown in nutrient solution containing Fe concentrations of 0 (Fe0), 20 (Fe20), 40 (Fe40), or 80 (Fe80) μM for 7 days. The Fe reductase activity of roots was higher in 03-3 plants grown under the Fe0, Fe20, and Fe40 treatments than in Bd-2 plants and the total Fe uptake was greater in 03-3 plants under the Fe40 treatment. GmFRD3a was much more highly expressed in the stem of 03-3 than in that of Bd-2, and significantly more iron was transported to 03-3 plant shoots during Fe0 treatment. Chlorosis in young leaves caused by Fe deficiency under the Fe0 and Fe20 treatments was alleviated by increased Fe concentration in shoots. Increased levels of active Fe in young 03-3 leaves under Fe-deprivation conditions (Fe0) and maintenance of stable Fe concentrations in 03-3 shoots subjected to Fe20, Fe40, and Fe80 treatments suggested that the P-efficient 03-3 cultivar is also Fe-efficient. It is suggested that 03-3 soybean cultivar should be a good resource for application to farm field.
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Dai
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Nanqi
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Guo
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Plant-Soil Interactions, MOE, Centre for Resource, Environment and Food Security, China Agricultural University, Beijing 100193, China. Electronic address: zuoym@cau.edu.cn.
authors.forename: Yuanmei
authors.initials: Y
authors.lastname: Zuo
mesh_terms: D001692:Biological Transport; D018533:Biomass; D038181:FMN Reductase / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D007501:Iron / Q000378:metabolism* / Q000494:pharmacology; D010758:Phosphorus / Q000378:metabolism*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D018520:Plant Shoots / Q000378:metabolism; D012987:Soil; D013025:Glycine max / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*
chemical_list: D012987:Soil; D010758:Phosphorus; D007501:Iron; D038181:FMN Reductase; C023903:ferric citrate iron reductase
keywords: Chlorosis; Fe and P interaction; Hydroponic; Nutrient deficiency; Soybean