title: Induced oxidative stress management in wounds through phenolic acids engineered fibrous protein: An in vitro assessment using polymorphonuclear (PMN) cells.
abstract: The present study explores the preparation, characterization and the role of phenolic acid tethered fibrous protein in the management of induced oxidative stress studied under in vitro conditions. In brief, the biomaterial is prepared by engineering the fibrous protein with dihydroxy and trihydroxy phenolic acid moieties and subjected to characterization to ensure the tethering. The resultant biomaterial studied for its efficacy as a free radical scavenger using polymorphonuclear (PMN) cells with induced oxidative stress and also as an agent for cell migration using fibroblasts cells. Results revealed that induced oxidative stress in PMN cells after exposure to UVB radiation managed well with the prepared biomaterial by reducing the levels of superoxide anion, oxygen and hydroxyl radicals. Further, the protein and the phenolic acid interaction supports the cell migration as evidenced from the scratch assay. In conclusion, though phenolic acids are well known for their antimicrobial and antioxidant potential, indenting these acids directly to the wounds is not sensible, but tethering to protein explored the scavenging activity as expected. The present study infers that phenolic acid engineered protein has a significant role in managing the imbalance in the redox state prevailing in wounds and supports the healing at appreciable level.
authors.affiliation: CSIR-Central Leather Research Institute, Adyar, Chennai 20, Tamil Nadu, India.
authors.forename: T
authors.initials: T
authors.lastname: Thiruselvi
authors.affiliation: CSIR-Central Leather Research Institute, Adyar, Chennai 20, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Thirupathi Kumara Raja
authors.affiliation: CSIR-Central Leather Research Institute, Adyar, Chennai 20, Tamil Nadu, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Shanuja
authors.affiliation: CSIR-Central Leather Research Institute, Adyar, Chennai 20, Tamil Nadu, India.
authors.forename: S
authors.initials: S
authors.lastname: Iswarya
authors.affiliation: CSIR-Central Leather Research Institute, Adyar, Chennai 20, Tamil Nadu, India. Electronic address: gnanamani3@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Gnanamani
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000378:metabolism; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D005110:Extracellular Space / Q000187:drug effects / Q000378:metabolism / Q000528:radiation effects; D062385:Hydroxybenzoates / Q000737:chemistry* / Q000494:pharmacology*; D017665:Hydroxyl Radical / Q000378:metabolism; D020782:Matrix Metalloproteinases / Q000378:metabolism; D009504:Neutrophils / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D018384:Oxidative Stress / Q000187:drug effects* / Q000528:radiation effects; D010851:Picrates / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D012596:Scleroproteins / Q000737:chemistry*; D013481:Superoxides / Q000378:metabolism; D013552:Swine; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D062385:Hydroxybenzoates; D010851:Picrates; D017382:Reactive Oxygen Species; D012596:Scleroproteins; D013481:Superoxides; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D020782:Matrix Metalloproteinases; C017616:phenolic acid
keywords: Cell migration; Fibrous protein; Induced oxidative stress; PMN cells; Phenolic acids