title: Differential gene expression and filamentation of Listeria monocytogenes 08-5923 exposed to sodium lactate and sodium diacetate.
abstract: This study reports the gene expression and filamentation in Listeria monocytogenes 08-5923 following exposure to food preservatives sodium lactate (NaL) and sodium diacetate (SD). L. monocytogenes 08-5923 was challenged with a mixture of NaL/SD, NaL or sodium acetate at 37 °C in tryptic soy broth. In the initial study, L. monocytogenes 08-5923 was exposed to NaL/SD for 24 h. The transcriptome was investigated by RNA sequencing. A stress response network was discovered in L. monocytogenes 08-5923, which is mediated by genes encoding two-component systems (hisJ, lisK, OmpR family gene, resE) and RNA polymerase factors (sigC, sigH). NaL/SD resulted in the down-regulation of genes in glycolysis (pykA, eno, fbaA, pgm) and up-regulation of genes in DNA repair (radC), cell division (ftsE) and cell structure synthesis (flagella synthesis: flgK, fliF, fliD). Filamentation was monitored by flow cytometry. NaL/SD mixture resulted in filamentation in L. monocytogenes 08-5923. Longer exposure was required to induce filamentation in L. monocytogenes for SD (24 h) than for NaL (8 h) when cells were exposed to individual salt. The quantitative real time PCR analysis revealed the down-regulation of ftsE in filamented cells of Listeria exposed to NaL or sodium acetate.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Xiaoji
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Urmila
authors.initials: U
authors.lastname: Basu
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada.
authors.forename: Petr
authors.initials: P
authors.lastname: Miller
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2P5, Canada. Electronic address: lynn.mcmullen@ualberta.ca.
authors.forename: Lynn M
authors.initials: LM
authors.lastname: McMullen
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D058491:Bacterial Load; D001426:Bacterial Proteins / Q000235:genetics; D004260:DNA Repair / Q000235:genetics; D004268:DNA-Binding Proteins / Q000235:genetics; D005407:Flagella / Q000235:genetics; D005516:Food Microbiology; D015870:Gene Expression; D020869:Gene Expression Profiling; D053263:Gene Regulatory Networks; D006019:Glycolysis / Q000235:genetics; D008089:Listeria monocytogenes / Q000187:drug effects* / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D060888:Real-Time Polymerase Chain Reaction; D017423:Sequence Analysis, RNA; D012808:Sigma Factor / Q000235:genetics; D019354:Sodium Lactate / Q000494:pharmacology*; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D000085:Acetates; D001426:Bacterial Proteins; D004268:DNA-Binding Proteins; C120328:SigH protein, bacteria; D012808:Sigma Factor; C081467:sigC protein, Bacteria; C548838:sodium diacetate; D019354:Sodium Lactate
keywords: Filamentation; Flow cytometry; Listeria; Preservatives; RNA sequencing