title: Development and application of micro-polysaccharide drug carriers incorporating doxorubicin and superparamagnetic iron oxide for bimodality treatment of hepatocellular carcinoma.
abstract: In this study, we demonstrated a novel polyelectrolyte microparticle, doxorubicin(DOX)-superparamagnetic iron oxide (SPIO)-chondroitin sulfate (CS)/chitosan (CHI)microparticles (MPs), as a drug delivery system for hepatic cancer treatment. We also investigated the properties of these microparticles through composition determination, formulation tests, in vitro study, and in vivo study. The results showed that our DOX-SPIO-CS/CHI MPs had an average diameter of 1.43±0.54μm and exhibited a spherical shape. The encapsulation efficiency of this drug carrier was approximately 31±8.07%, according to our spectroscopic determination. The results of release profile test revealed the sustained-release behavior of DOX-SPIO-CS/CHI MPs, which released 51.5% of DOX within 48h of the testing period. According to the results of a cell viability assay and an animal study, the DOX-SPIO-CS/CHI MPs exhibited stronger cytotoxicity than did free DOX when it was administered to Hep G2 and Huh-6 human liver cancer cell lines in vitro and to nude mice of Hep G2/Huh-6-bearing mice model in vivo.
authors.affiliation: Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC.
authors.forename: Sung-Ling
authors.initials: SL
authors.lastname: Tang
authors.affiliation: Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC; Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC; Adjunct Appointment to the Department of Biomedical Engineering, National Defense Medical Center, Taipei, Taiwan, ROC. Electronic address: mybai@mail.ntust.edu.tw.
authors.forename: Meng-Yi
authors.initials: MY
authors.lastname: Bai
authors.affiliation: Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC.
authors.forename: Jyun-Yi
authors.initials: JY
authors.lastname: Wang
authors.affiliation: Biomedical Engineering Program, Graduate Institute of Applied Science and Technology, National Taiwan University of Science and Technology, Taipei, Taiwan, ROC.
authors.forename: Po-Da
authors.initials: PD
authors.lastname: Hong
mesh_terms: D000818:Animals; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy*; D045744:Cell Line, Tumor; D002470:Cell Survival; D048271:Chitosan / Q000737:chemistry; D002809:Chondroitin Sulfates / Q000737:chemistry; D004317:Doxorubicin / Q000737:chemistry*; D004337:Drug Carriers; D005260:Female; D005290:Ferric Compounds / Q000737:chemistry*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy*; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D009368:Neoplasm Transplantation; D010316:Particle Size; D011134:Polysaccharides / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D004337:Drug Carriers; D005290:Ferric Compounds; D011134:Polysaccharides; C000499:ferric oxide; D004317:Doxorubicin; D002809:Chondroitin Sulfates; D048271:Chitosan
keywords: Chitosan; Doxorubicin; Electrospray; Microparticles; Nanotechnology; SPIO