title: Vibrio injenensis sp. nov., isolated from human clinical specimens.
abstract: Vibrio species are well known as motile, mostly oxidase-positive, facultative anaerobic Gram-negative bacteria. They are abundant in aquatic environments and are a common cause of human infections including diarrhea, soft tissue diseases, and bacteremia. Here, two Gram-negative bacteria, designated M12-1144Tand M12-1181, were isolated from human clinical specimens and identified using a polyphasic taxonomic approach. Phylogenetic study based on 16S rRNA gene sequence analysis revealed that the isolates belong to the genus Vibrio, and are closely related to Vibrio metschnikovii KCTC 32284T(98.3%) and Vibrio cincinnatiensis KCTC 2733T(97.8%). The major fatty acids were summed feature 3 (C16:1ω7c/C16:1ω6c, 38.0%), C16:0(23.0%), and summed feature 8 (C18:1ω7c or C18:1ω6c, 19.3%) and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, and phosphatidylethanolamine. The G + C content of the genomic DNA was determined to be 44.1 mol%. DNA-DNA relatedness between the two newly isolated strains and V. metschnikovii KCTC 32284Tand V. cincinnatiensis KCTC 2733Twas between 42.6 to 47.5%. The similarities of genome-to-genome distance between M12-1144Tand related species ranged from 18.4-54.8%. Based on these results, a new species of the genus Vibrio, Vibrio injenensis is proposed. The type strain is M12-1144T(=KCTC 32233T=JCM 30011T).
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: Jayoung
authors.initials: J
authors.lastname: Paek
authors.affiliation: Department of Laboratory Medicine, Inje University College of Medicine, Busan, 614-735, Republic of Korea.
authors.forename: Jeong Hwan
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: Yeseul
authors.initials: Y
authors.lastname: Shin
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea.
authors.forename: In-Soon
authors.initials: IS
authors.lastname: Park
authors.affiliation: Vitabio, Inc., Daejeon, 305-500, Republic of Korea.
authors.forename: Hongik
authors.initials: H
authors.lastname: Kim
authors.affiliation: Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju, 501-759, Republic of Korea.
authors.forename: Joong-Ki
authors.initials: JK
authors.lastname: Kook
authors.affiliation: Department of Food Science and Biotechnology, Dongguk University-Seoul, Goyang, 10326, Republic of Korea.
authors.forename: Seok-Seong
authors.initials: SS
authors.lastname: Kang
authors.affiliation: Human Derived Material Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-333, Republic of Korea.
authors.forename: Dae-Soo
authors.initials: DS
authors.lastname: Kim
authors.affiliation: Human Derived Material Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-333, Republic of Korea.
authors.forename: Kun-Hyang
authors.initials: KH
authors.lastname: Park
authors.affiliation: Korean Collection for Type Cultures/ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon, 305-806, Republic of Korea. yhchang@kribb.re.kr.
authors.forename: Young-Hyo
authors.initials: YH
authors.lastname: Chang
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D014733:Vibrio / Q000145:classification / Q000302:isolation & purification* / Q000378:metabolism; D014735:Vibrio Infections / Q000382:microbiology*
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S
keywords: Human clinical specimens; Polyphasic taxonomy; Vibrio injenensis sp. nov