title: CpG Oligodeoxynucleotides Induce Differential Cytokine and Chemokine Gene Expression Profiles in Dapulian and Landrace Pigs.
abstract: Oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODN) mimic the immunostimulatory activity of microbial DNA by interacting with Toll-like receptor 9 (TLR9) to activate both the innate and adaptive immune responses in different species. However, few studies have been published to compare the effects of CpG ODN on different pig breeds. Therefore, in this study, whole blood gene expression profiles of DPL and Landrace pigs treated with CpG ODN were studied using RNA-seq technology. Five Hundred differentially expressed genes (DEGs) were identified between the two breeds. DPL pigs had significantly higher number of immune-relevant DEGs than the Landrace pigs after CpG ODN treatment. Pathway analysis showed that cytokine-cytokine receptor interaction and chemokine signaling pathway were the major enriched pathways of the immune-relevant DEGs. Furtherin vitroexperiments showed that PBMCs of the DPL pigs had significantly higher levels of TLR9 mRNA than those of the Landrace pigs, both before and after CpG ODN stimulation. Cytokine and chemokine induction in the PBMCs of both breeds were also measured after CpG ODN stimulation. Our data showed that mRNA levels of cytokines (IFNα, IL8, IL12 p40) and chemokines (CXCL9, CXCL13) were significantly higher in the PBMCs of the DPL pigs than those of the Landrace pigs. Taken together, our data provide new information regarding the pig breed difference in response to CpG ODN stimulation and that higher levels of TLR9 mRNA in DPL pigs may be a major contributor for disease resistance.
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University Tai'an, China.
authors.forename: Jiaqing
authors.initials: J
authors.lastname: Hu
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University Tai'an, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yang
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University Tai'an, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University Tai'an, China.
authors.forename: Chuanhao
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University Tai'an, China.
authors.forename: Yongqing
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Animal Genetics and Breeding, College of Animal Science and Technology, Shandong Agricultural University Tai'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
keywords: CpG ODN; chemokines; cytokines; pig; transcriptome