title: Periostin is required for matricellular localization of CCN3 in periodontal ligament of mice.
abstract: CCN3 is a matricellular protein that belongs to the CCN family. CCN3 consists of 4 domains: insulin-like growth factor-binding protein-like domain (IGFBP), von Willebrand type C-like domain (VWC), thrombospondin type 1-like domain (TSP1), and the C-terminal domain (CT) having a cysteine knot motif. Periostin is a secretory protein that binds to extracellular matrix proteins such as fibronectin and collagen. In this study, we found that CCN3 interacted with periostin. Immunoprecipitation analysis revealed that the TSP1-CT interacted with the 4 repeats of the Fas 1 domain of periostin. Immunofluorescence analysis showed co-localization of CCN3 and periostin in the periodontal ligament of mice. In addition, targeted disruption of the periostin gene in mice decreased the matricellular localization of CCN3 in the periodontal ligament. Thus, these results indicate that periostin was required for the matricellular localization of CCN3 in the periodontal ligament, suggesting that periostin mediated an interaction between CCN3 and the extracellular matrix.
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-33, Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.
authors.forename: Issei
authors.initials: I
authors.lastname: Takayama
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-33, Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.|Division of Molecular and Developmental Biology, National Institute of Genetics, Mishima, Shizuoka, 411-8540, Japan.
authors.forename: Hideyuki
authors.initials: H
authors.lastname: Tanabe
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-33, Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan.|Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Shimotsuke-Shi, Tochigi, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Nishiyama
authors.affiliation: Pathophysiological and Health Science Team, Imaging Platform and Innovation Group, Division of Bio-Function Dynamics Imaging, RIKEN Center for Life Science Technologies, 6-7-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Ito
authors.affiliation: Division of Oral Health Science, Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, 060-8586, Japan.
authors.forename: Norio
authors.initials: N
authors.lastname: Amizuka
authors.affiliation: Division of Oral Health Science, Department of Developmental Biology of Hard Tissue, Graduate School of Dental Medicine, Hokkaido University, Sapporo, 060-8586, Japan.|Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Department of Bone Metabolism, School of Stomatology, Shandong University, Wenhua West Road 44-1, Jinan, 250012, China.
authors.forename: Minqi
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Molecular Pathology, Graduate School of Tokyo Medical and Dental University, Tokyo, 113-8519, Japan.|Department of Nursing Science, Faculty of Human Care, Tohto College of Health Sciences, Saitama, Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Katsube
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-33, Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan. isao.kii@riken.jp.|Pathophysiological and Health Science Team, Imaging Platform and Innovation Group, Division of Bio-Function Dynamics Imaging, RIKEN Center for Life Science Technologies, 6-7-3 Minatojima-minamimachi, Chuo-ku, Kobe, Hyogo, 650-0047, Japan. isao.kii@riken.jp.
authors.forename: Isao
authors.initials: I
authors.lastname: Kii
authors.affiliation: Department of Biological Information, Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259-B-33, Nagatsuta-cho, Midori-ku, Yokohama, 226-8501, Japan. akudo@bio.titech.ac.jp.
authors.forename: Akira
authors.initials: A
authors.lastname: Kudo
keywords: CCN3; Periodontal ligament; Periostin