title: Molecular characterization of Salmonella Typhimurium isolated in Brazil by CRISPR-MVLST.
abstract: CRISPR-multi-locus virulence sequence typing (CRISPR-MVLST) was performed to type 92 S. Typhimurium strains isolated from humans and food sources between 1983 and 2013 in Brazil and assess the suitability of this methodology comparing it with PFGE already used for subtyping the same strains. Among the 92 S. Typhimurium strains studied, we identified 25 CRISPR1 alleles, 27 CRISPR2 alleles, 2 fimH alleles and 3 sseL alleles showing that the genetic variability is much higher in the CRISPRs loci than in the virulence genes. The CRISPR-MVLST analysis provided similar results to the PFGE previously published used to type the same set of strains, demonstrating that CRISPR-MVLST is a very efficient approach for subtyping S. Typhimurium serovar and can be used to complement and validate results obtained by the PFGE methodology.
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Av. do Café s/n°, Ribeirão Preto, SP, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Almeida
authors.affiliation: Centro de Laboratório Regional de Ribeirão Preto, Instituto Adolfo Lutz, Rua Minas 877, Ribeirão Preto, SP, Brazil.
authors.forename: Marta Inês Cazentini
authors.initials: MIC
authors.lastname: Medeiros
authors.affiliation: Laboratório de Enterobactérias, FIOCRUZ/Fundação Instituto Oswaldo Cruz, Avenida Brasil, 4365, Pavilhão Rocha Lima, 3°andar, Manguinhos, 21040-360 Rio de Janeiro, RJ, Brazil.
authors.forename: Dália Dos Prazeres
authors.initials: DDP
authors.lastname: Rodrigues
authors.affiliation: Office of Regulatory Science, Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, MD, United States.
authors.forename: Marc W
authors.initials: MW
authors.lastname: Allard
authors.affiliation: Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Av. do Café s/n°, Ribeirão Preto, SP, Brazil. Electronic address: jufalcao@fcfrp.usp.br.
authors.forename: Juliana Pfrimer
authors.initials: JP
authors.lastname: Falcão
mesh_terms: D000483:Alleles; D015373:Bacterial Typing Techniques; D001938:Brazil; D064112:Clustered Regularly Interspaced Short Palindromic Repeats / Q000235:genetics*; D004269:DNA, Bacterial / Q000302:isolation & purification*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D056426:Genetic Loci; D006801:Humans; D058885:Multilocus Sequence Typing; D012486:Salmonella typhimurium / Q000235:genetics* / Q000302:isolation & purification*; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial
keywords: CRISPR-MVLST; PFGE; Salmonella Typhimurium