title: Development of an immunoassay using an anti-wogonin glucuronide monoclonal antibody.
abstract: Wogonin 7-O-β-D-glucuronide (Wgn) is a bioactive flavone present in the dried root of Scutellaria baicalensis Georgi. To generate a monoclonal antibody (MAb) against Wgn, BALB/c mice injected with Wgn-bovine serum albumin yielded splenocytes that we fused with SP2/0 myeloma cells using the polyethylene glycol method. We obtained a hybridoma designated 315A that produced a MAb reactive to Wgn. The anti-Wgn MAb 315A was applied to an indirect competitive enzyme-linked immunosorbent assay (icELISA) to quantify Wgn. Subsequent validation revealed that icELISA using the 315A anti-Wgn MAb is an accurate and reliable method for the quantification of Wgn in S. baicalensis.
authors.affiliation: a Department of Pharmacognosy , Graduate School of Pharmaceutical Sciences, Kyushu University , Fukuoka , Japan.
authors.forename: Madan Kumar
authors.initials: MK
authors.lastname: Paudel
authors.affiliation: a Department of Pharmacognosy , Graduate School of Pharmaceutical Sciences, Kyushu University , Fukuoka , Japan.
authors.forename: Seiichi
authors.initials: S
authors.lastname: Sakamoto
authors.affiliation: b Department of Natural Product Chemistry , Graduate School of Pharmaceutical Sciences, Kyushu University , Fukuoka , Japan.
authors.forename: Le Van
authors.initials: LV
authors.lastname: Huy
authors.affiliation: a Department of Pharmacognosy , Graduate School of Pharmaceutical Sciences, Kyushu University , Fukuoka , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Tanaka
authors.affiliation: b Department of Natural Product Chemistry , Graduate School of Pharmaceutical Sciences, Kyushu University , Fukuoka , Japan.
authors.forename: Tomofumi
authors.initials: T
authors.lastname: Miyamoto
authors.affiliation: c Department of Plant Resources for Medicine , Graduate School of Pharmaceutical Sciences, Showa Pharmaceutical University , Tokyo , Japan.
authors.forename: Akihito
authors.initials: A
authors.lastname: Takano
authors.affiliation: a Department of Pharmacognosy , Graduate School of Pharmaceutical Sciences, Kyushu University , Fukuoka , Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Morimoto
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000937:Antigen-Antibody Reactions; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D044950:Flavanones / Q000032:analysis / Q000276:immunology*; D020719:Glucuronides / Q000032:analysis / Q000276:immunology*; D051379:Mice; D008807:Mice, Inbred BALB C
chemical_list: D000911:Antibodies, Monoclonal; D044950:Flavanones; D020719:Glucuronides; C465289:wogonin-7-glucuronide
keywords: Scutellaria baicalensis; Wogonin 7-O-β-D-glucuronide; indirect competitive enzyme-linked immunosorbent assay; monoclonal antibody