title: Asymmetrical labeling of giant phospholipid vesicles.
abstract: The method for labeling of inner membrane leaflet in unilamellar giant POPC vesicles was developed and characterised. Symmetrically NBD-PC labeled vesicles were treated by sodium dithionite, which undergoes an irreversible chemical reaction with NBD-PC molecule making it non-fluorescent. After the addition of dithionite the fluorescence on single vesicles as well as on vesicle suspension showed a 50 % decrease of its initial value corresponding to marker quenching in the outer leaflet. Hence, flourimetry as well as flourescence microscopy prove that dithionite quenching is a suitable method to induce an asymmetrical labeling of the NBD-PC marked giant vesicles.
authors.affiliation: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva 2, 1104 Ljubljana, Slovenia, Slovenia.
authors.forename: Gregor
authors.initials: G
authors.lastname: Gomišček
authors.affiliation: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva 2, 1104 Ljubljana, Slovenia, Slovenia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Arrigler
authors.affiliation: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva 2, 1104 Ljubljana, Slovenia, Slovenia.
authors.forename: Marjan
authors.initials: M
authors.lastname: Gros
authors.affiliation: Pedriatric Clinics, Vrazov trg 1, 1104 Ljubljana, Slovenia, Slovenia.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Zupančič
authors.affiliation: Institute of Biophysics, Faculty of Medicine, University of Ljubljana, Lipičeva 2, 1104 Ljubljana, Slovenia, Slovenia.
authors.forename: Saša
authors.initials: S
authors.lastname: Svetina
keywords: Key words giant vesicles; asymmetrical labeling; fluorescence; sodium dithionite