title: Polymorphism variant of MnSOD A16V and risk of female infertility in northern Iran.
abstract: OBJECTIVE
  Infertility is a disease of the reproductive system defined by inability to conceive after having regular unprotected intercourse. Both environmental and genetic factors can be involved in female infertility. Manganese superoxide dismutase (MnSOD) is a crucial mitochondrial antioxidant enzyme that has a key role in cellular defense against agents that induce oxidative stress. The present study was aimed to evaluate the MnSOD A16V gene polymorphism in female infertility in northern Iran.
  
  
  METHODS
  Samples were obtained from 150 patients diagnosed with female infertility and 150 controls and genotyped by the polymerase chain reaction-restriction fragment length polymorphism method.
  
  
  RESULTS
  The MnSOD genotype frequencies amongst the 150 cases were A/A = 27.3%, A/V = 69.4%, and V/V = 3.3%; the A and V allele frequencies were 62% and 38%, respectively. The MnSOD genotype frequencies amongst the 150 controls were A/A = 33.3%, A/V = 48.0%, and V/V = 18.7%; the A and V allele were 57% and 43%, respectively. We observed a significant difference in genotype distributions of MnSOD A16V polymorphism between patients and controls (p = 0.0001).
  
  
  CONCLUSIONS
  It is suggested that the MnSOD A16V polymorphism may be associated with a risk of female infertility in northern Iran. More studies should be considered with a larger number of patients and controls to confirm our results.
authors.affiliation: Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. Electronic address: mostafapournourali@yahoo.com.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Pournourali
authors.affiliation: Agriculture Biotechnology Research Institute of Iran (ABRII), Branches of North Region of Iran, Department of Genomics and Animal, Rasht, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Tarang
authors.affiliation: Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
authors.forename: Sarah Farzadi
authors.initials: SF
authors.lastname: Haghighi
authors.affiliation: Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Yousefi
authors.affiliation: Cellular and Molecular Research Center, Faculty of Medical Sciences, Guilan University of Medical Sciences, Rasht, Iran.
authors.forename: Mohammad Hadi
authors.initials: MH
authors.lastname: Bahadori
mesh_terms: D000328:Adult; D000483:Alleles; D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency; D006801:Humans; D007247:Infertility, Female / Q000235:genetics*; D007492:Iran; D016133:Polymerase Chain Reaction; D020641:Polymorphism, Single Nucleotide; D012306:Risk; D013482:Superoxide Dismutase / Q000097:blood / Q000235:genetics*
chemical_list: D013482:Superoxide Dismutase
keywords: MnSOD; female infertility; gene polymorphism