title: Differentiation of mesenchymal stem cells from human amniotic fluid to vascular endothelial cells.
abstract: Endothelial dysfunction is a principle feature of vascular-related disease. Endothelial cells have been acquired for the purposes of the restoration of damaged tissue in therapeutic angiogenesis. However, their use is limited by expansion capacity and the small amount of cells that are obtained. Human amniotic fluid mesenchymal stem cells (hAF-MSCs) are considered an important source for vascular tissue engineering. In this study, hAF-MSCs were characterized and then induced in order to differentiate into the endothelial-like cells. Human amniotic fluid cells (hAFCs) were obtained from amniocentesis at the second trimester of gestation. The cells were characterized as mesenchymal stem cells by flow cytometry. The results showed that the cells were positive for mesenchymal stem cell markers CD44, CD73, CD90 and HLA-ABC, and negative for CD31, Amniotic fluid stem cells marker: CD117, anti-human fibroblasts, HLA-DR and hematopoietic differentiation markers CD34 and CD45. The hAF-MSCs were differentiated into endothelial cells under the induction of vascular endothelial growth factor (VEGF) and analyzed for the expression of the endothelial-specific markers and function. The expression of the endothelial-specific markers was determined by reverse transcriptase-quantitative PCR (RT-qPCR), while immunofluorescent analysis demonstrated that the induced hAF-MSCs expressed von Willebrand factor (vWF), vascular endothelial growth factor receptor 2 (VEGFR2), CD31 and endothelial nitric oxide synthase (eNOS). The network formation assay showed that the induced hAF-MSCs formed partial networks. All results indicated that hAF-MSCs have the potential to be differentiated into endothelial-like cells, while human amniotic fluid might be a suitable source of MSCs for vascularized tissue engineering.
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Waleephan
authors.initials: W
authors.lastname: Tancharoen
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand. Electronic address: sirinda001@hotmail.com.
authors.forename: Sirinda
authors.initials: S
authors.lastname: Aungsuchawan
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Peraphan
authors.initials: P
authors.lastname: Pothacharoen
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Runchana
authors.initials: R
authors.lastname: Markmee
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Suteera
authors.initials: S
authors.lastname: Narakornsak
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Junjira
authors.initials: J
authors.lastname: Kieodee
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Nonglak
authors.initials: N
authors.lastname: Boonma
authors.affiliation: Department of Anatomy, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand.
authors.forename: Witoon
authors.initials: W
authors.lastname: Tasuya
mesh_terms: D000653:Amniotic Fluid / Q000166:cytology; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology; D061307:Human Umbilical Vein Endothelial Cells / Q000502:physiology*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D042461:Vascular Endothelial Growth Factor A / Q000502:physiology
chemical_list: C467484:VEGFA protein, human; D042461:Vascular Endothelial Growth Factor A
keywords: Endothelial differentiation; Human amniotic fluid; Human amniotic fluid derived-mesenchymal stem cells (hAF-MSCs); Mesenchymal stem cells