title: Uterine and placental expression of HPGD in cows during pregnancy and release of fetal membranes.
abstract: 15-Hydroxyprostaglandin dehydrogenase (HPGD) plays a key role in prostaglandins (PGs) catabolism. Its expression and activity appear to be regulated by progesterone (P4). We investigated the HPGD mRNA-expression and protein localization in placentomes and interplacental uterine sites throughout gestation (Study I), and after fetal membranes retention (RFM) compared with normally delivered fetal membranes (DFM) (Study II). Furthermore, we analyzed the influence of aglepristone (AP), dexamethasone (GC) or cloprostenol (CP), on HPGD expression in bovine placentomes (Study III). Tissues from late gestation (D272) and at normal term (NT) served as controls. HPGD was highest in all sites at the beginning of pregnancy and at (NT). Following induced parturition HPGD was lower after (AP) and (GC) compared with (NT), and was similar in RFM and DFM. Placentomes stained primarily in fetal compartments; interplacentomal signals were observed in endometrial glandular and luminal epithelium. Results indicate that HPGD may play a role during establishment and termination of gestation.
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland. Electronic address: jessica.vonhof@uzh.ch.
authors.forename: Jessica
authors.initials: J
authors.lastname: von Hof
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland. Electronic address: nelesprekeler@gmx.de.
authors.forename: Nele
authors.initials: N
authors.lastname: Sprekeler
authors.affiliation: Clinic for Obstetrics, Gynecology and Andrology of Large and Small Animals, Faculty of Veterinary Medicine, Justus-Liebig-University Giessen, 35392 Giessen, Germany. Electronic address: gerhard.schuler@vetmed.uni-giessen.de.
authors.forename: Gerhard
authors.initials: G
authors.lastname: Schuler
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland. Electronic address: boosa@vetanat.uzh.ch.
authors.forename: Alois
authors.initials: A
authors.lastname: Boos
authors.affiliation: Institute of Veterinary Anatomy, Vetsuisse Faculty, University of Zurich, Winterthurerstrasse 260, 8057 Zurich, Switzerland. Electronic address: kowalewskipl@yahoo.de.
authors.forename: Mariusz P
authors.initials: MP
authors.lastname: Kowalewski
mesh_terms: D000818:Animals; D002417:Cattle; D005321:Extraembryonic Membranes / Q000378:metabolism*; D005260:Female; D015971:Gene Expression Regulation, Enzymologic; D006911:Hydroxyprostaglandin Dehydrogenases / Q000235:genetics* / Q000378:metabolism*; D010920:Placenta / Q000378:metabolism*; D011247:Pregnancy; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014599:Uterus / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D006911:Hydroxyprostaglandin Dehydrogenases; C035859:15-hydroxyprostaglandin dehydrogenase
keywords: Bovine; Cattle; Gestation; HPGD; Placenta; Retained fetal membranes; Uterus