title: Rapid and sensitive method to assess human viral pollution in shellfish using infectious F-specific RNA bacteriophages: Application to marketed products.
abstract: F-specific RNA bacteriophages (FRNAPH) have been used as indicators of environmental fecal pollution for many years. While FRNAPH subgroup I (FRNAPH-I) are not host specific, some FRNAPH-II and -III strains appear specific to human pollution. Because a close relationship has been observed between FRNAPH-II genome and human norovirus (NoV) in shellfish, and because FRNAPH infectivity can easily be investigated unlike that of NoV, the detection of human infectious FRNAPH could therefore provide a valuable tool for assessing viral risk. In this study, an integrated cell culture real-time RT-PCR method has been developed to investigate infectious FRNAPH subgroup prevalence in oysters. This rapid screening method appears more sensitive than E. coli or NoV genome detection, and allows an FRNAPH subgroup present in low concentrations (0.05 PFU/g of oyster) to be detected in the presence of another 1000 times more concentrated, without any dissection step. Its application to marketed oysters (n = 135) over a 1-year period has allowed to identify the winter peak classically described for NoV or FRNAPH accumulation. Infectious FRNAPH were detected in 34% of batches, and 7% were suspected of having a human origin. This approach may be helpful to evaluate oyster's depuration processes, based on an infectious viral parameter.
authors.affiliation: Université de Lorraine, LCPME (Laboratoire de Chimie Physique et Microbiologie pour l'Environnement), UMR 7564, Faculté de Pharmacie, Nancy F-54000, France; CNRS, LCPME, UMR 7564, Nancy F-54000, France; Institut Jean Barriol, Université de Lorraine, Faculté des Sciences et Technologies, Vandœuvre-lès-Nancy F-54506, France.
authors.forename: Cédric
authors.initials: C
authors.lastname: Hartard
authors.affiliation: Université de Lorraine, LCPME (Laboratoire de Chimie Physique et Microbiologie pour l'Environnement), UMR 7564, Faculté de Pharmacie, Nancy F-54000, France; CNRS, LCPME, UMR 7564, Nancy F-54000, France; Institut Jean Barriol, Université de Lorraine, Faculté des Sciences et Technologies, Vandœuvre-lès-Nancy F-54506, France.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Banas
authors.affiliation: Université de Lorraine, LCPME (Laboratoire de Chimie Physique et Microbiologie pour l'Environnement), UMR 7564, Faculté de Pharmacie, Nancy F-54000, France; CNRS, LCPME, UMR 7564, Nancy F-54000, France; Institut Jean Barriol, Université de Lorraine, Faculté des Sciences et Technologies, Vandœuvre-lès-Nancy F-54506, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Rivet
authors.affiliation: Actalia, Food Safety Department, Saint-Lô F-50000, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Boudaud
authors.affiliation: Université de Lorraine, LCPME (Laboratoire de Chimie Physique et Microbiologie pour l'Environnement), UMR 7564, Faculté de Pharmacie, Nancy F-54000, France; CNRS, LCPME, UMR 7564, Nancy F-54000, France; Institut Jean Barriol, Université de Lorraine, Faculté des Sciences et Technologies, Vandœuvre-lès-Nancy F-54506, France. Electronic address: christophe.gantzer@univ-lorraine.fr.
authors.forename: Christophe
authors.initials: C
authors.lastname: Gantzer
mesh_terms: D000818:Animals; D003257:Consumer Product Safety; D004787:Environmental Pollution; D004926:Escherichia coli / Q000235:genetics; D005243:Feces / Q000821:virology; D006801:Humans; D057230:Limit of Detection; D029322:Norovirus / Q000235:genetics; D010125:Ostreidae / Q000821:virology*; D012317:RNA Phages / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D012621:Seasons; D012680:Sensitivity and Specificity; D012758:Shellfish / Q000821:virology*; D010948:Viral Plaque Assay; D014871:Water Microbiology; D014876:Water Pollution
keywords: F-specific RNA phages; Fecal pollution; ICC-PCR; Oysters; Prevalence