title: Quercetin Confers Tumoricidal Activity Through Multipathway Mechanisms in A N-Methylnitrosourea Rat Model of Colon Cancer
abstract: Objective: This research was conducted to explore mechanisms behind the potency of quercetin in inhibiting
  colon cancer induced in an experimental model. Materials and Methods: Forty adult male rats of Wistar strain were
  distributed into 4 groups; a negative control group, a colon cancer bearing group, a quercetin-treated group and a
  5-fluorouracil (5-FU)-treated group. Serum TAG72 and GAL3 levels were quantified by ELISA. Colonic Wnt5a and
  Axin-1 gene expression was estimated by PCR. In addition, colonic tissues were subjected to immunohistochemical
  examination of Bax expression and histological investigation of histopathological alterations. Results: Quercetin elicited
  significant reduction in serum TAG72 and GAL3 levels, in addition to significant suppression of colonic Wnt5a gene
  expression and amplification of colonic Axin-1 gene expression. Also, it caused moderate positive reaction for Bax in
  mucosal epithelium. Conclusion: The present research provides experimental evidence about the activity of quercetin
  in the colon cancer of rats. Inhibitory effects on cancer development might be ascribable to regulatory action on Wnt
  signaling and induction of apoptosis.
authors.affiliation: Hormones Department, National Research Centre, Giza, Egypt. Email: hanaaomr@yahoo.com
authors.forename: Hanaa H
authors.initials: HH
authors.lastname: Ahmed
authors.forename: Hadeer A
authors.initials: HA
authors.lastname: Aglan
authors.forename: Asmaa M
authors.initials: AM
authors.lastname: Zaazaa
authors.forename: Aziza B
authors.initials: AB
authors.lastname: Shalby
authors.forename: Sayed A
authors.initials: SA
authors.lastname: El Toumy
keywords: Colon cancer; quercetin; 5-fluorouracil; Wnt signaling; rats