title: Electrophoretic mobility of a monotopic membrane protein inserted into the top of supported lipid bilayers.
abstract: We have studied the translational migration of a monotopic membrane protein, the bacterial sulfide quinone reductase (SQR) in supported n-bilayers ([Formula: see text]) under the influence of an electric field parallel to the membrane plane. The direction of the migration changes when the charge of the protein changes its sign. Measuring mobilities at different pH enables us to gain experimental physico-chemical data on SQR as its isoelectric point and its estimated oligomeric state (at least trimeric) when inserted in a lipid membrane. Consequently, in addition to the migration study of membrane proteins in a lipid environment, this experimental system, previously used with a transmembrane protein, is thus suitable to define membrane protein properties in conditions approaching the native ones (in the absence of detergent).
authors.affiliation: Doctoral school for Science and Technology, platform for research in Nanosciences and Nanotechnology, Lebanese University, Campus Pierre Gemayel, BP 90239, Fanar-Metn, Lebanon.|Aix-Marseille Univ, CINaM-CNRS, UMR7325, 13288, Marseille, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Harb
authors.affiliation: Aix Marseille Univ, CNRS, BIP UMR 7281, 13402, Marseille, France.
authors.forename: Marie-Thérèse
authors.initials: MT
authors.lastname: Giudici-Orticoni
authors.affiliation: Aix Marseille Univ, CNRS, BIP UMR 7281, 13402, Marseille, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Guiral
authors.affiliation: Aix-Marseille Univ, CINaM-CNRS, UMR7325, 13288, Marseille, France. tinland@cinam.univ-mrs.fr.
authors.forename: Bernard
authors.initials: B
authors.lastname: Tinland
mesh_terms: D001419:Bacteria / Q000201:enzymology; D004560:Electricity; D004586:Electrophoresis; D006863:Hydrogen-Ion Concentration; D007526:Isoelectric Point; D008051:Lipid Bilayers / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry*; D020836:Protein Structure, Quaternary; D011808:Quinone Reductases / Q000737:chemistry
chemical_list: D008051:Lipid Bilayers; D008565:Membrane Proteins; D011808:Quinone Reductases; C073412:sulfide quinone reductase
keywords: Living systems: Biomimetic Systems