title: A new saponin from Acanthopanax koreanum with anti-inflammatory activity.
abstract: Twelve saponins were isolated from the leaves of Acanthopanax koreanum, including one new lupane-type triterpene glycoside, named acankoreoside R (1), together with 11 known triterpenoid saponins (2-12). Their structures were elucidated by 1D and 2D nuclear magnetic resonance (NMR), mass spectroscopic data (MS). All of the fractions and isolated saponins were evaluated for anti-inflammatory activities in lipopolysaccharide (LPS)-stimulated bone marrow-derived dendritic cells (BMDCs) by ELISA. Among them, compounds 1-5, 7, 10, and 12 showed strong inhibitions towards interleukin-12 (IL-12) production with IC50values ranging from 1.59 to 5.46 µM. Other compounds were weak or inactive toward IL-12 p40 production.
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea.
authors.forename: Le Duc
authors.initials: LD
authors.lastname: Dat
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.|Institute of Marine Biochemistry (IMBC), Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Viet Nam.
authors.forename: Nguyen Phuong
authors.initials: NP
authors.lastname: Thao
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.
authors.forename: Bui Thi Thuy
authors.initials: BT
authors.lastname: Luyen
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea.
authors.forename: Bui Huu
authors.initials: BH
authors.lastname: Tai
authors.affiliation: College of Pharmacy, Catholic University of Daegu, Gyeongsan, 38430, Republic of Korea.
authors.forename: Mi Hee
authors.initials: MH
authors.lastname: Woo
authors.affiliation: School of Medicine, Brain Korea 21 PLUS Programand Institute of Medical Science, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Zahid
authors.initials: Z
authors.lastname: Manzoor
authors.affiliation: School of Medicine, Brain Korea 21 PLUS Programand Institute of Medical Science, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Irshad
authors.initials: I
authors.lastname: Ali
authors.affiliation: School of Medicine, Brain Korea 21 PLUS Programand Institute of Medical Science, Jeju National University, Jeju, 690-756, Republic of Korea.
authors.forename: Young Sang
authors.initials: YS
authors.lastname: Koh
authors.affiliation: College of Pharmacy, Chungnam National University, Daejeon, 34134, Republic of Korea. yhk@cnu.ac.kr.
authors.forename: Young Ho
authors.initials: YH
authors.lastname: Kim
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D001854:Bone Marrow Cells / Q000187:drug effects; D003713:Dendritic Cells / Q000187:drug effects; D028001:Eleutherococcus / Q000737:chemistry*; D018664:Interleukin-12 / Q000096:biosynthesis; D008070:Lipopolysaccharides / Q000494:pharmacology; D009682:Magnetic Resonance Spectroscopy; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D008958:Models, Molecular; D018515:Plant Leaves / Q000737:chemistry; D012503:Saponins / Q000302:isolation & purification* / Q000494:pharmacology*; D013329:Structure-Activity Relationship
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D008070:Lipopolysaccharides; D012503:Saponins; C000616596:acankoreoside R; D018664:Interleukin-12
keywords: Acanthopanaxkoreanum; IL-12; Lupane saponin