title: Quantitative assessment of the diagnostic role of FHIT promoter methylation in non-small cell lung cancer.
abstract: Aberrant methylation of CpG islands acquired in promoter regions plays an important role in carcinogenesis. Accumulated evidence demonstrates FHIT gene promoter hyper-methylation is involved in non-small cell lung cancer (NSCLC). To test the diagnostic ability of FHIT methylation status on NSCLC, thirteen studies, including 2,119 samples were included in our meta-analysis. Simultaneously, four independent DNA methylation datasets from TCGA and GEO database were analyzed for validation. The pooled odds ratio of FHIT promoter methylation in cancer samples was 3.43 (95% CI: 1.85 to 6.36) compared with that in controls. In subgroup analysis, significant difference of FHIT gene promoter methylation status in NSCLC and controls was found in Asians but not in Caucasian population. In validation stage, 950 Caucasian samples, including 126 paired samples from TCGA, 568 cancer tissues and 256 normal controls from GEO database were analyzed, and all 8 CpG sites near the promoter region of FHIT gene were not significantly differentially methylated. Thus the diagnostic role of FHIT gene in the lung cancer may be relatively limited in the Caucasian population but useful in the Asians.
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Geng
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Weilin
authors.initials: W
authors.lastname: Pu
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Chest Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China.
authors.forename: Zhouyi
authors.initials: Z
authors.lastname: Lu
authors.affiliation: Department of Chest Surgery, Shanghai Pulmonary Hospital, Shanghai 200433, China.
authors.forename: An
authors.initials: A
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Lixing
authors.initials: L
authors.lastname: Tan
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Sidi
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Bioengineering, University of California at San Diego, La Jolla, CA 92093, USA.
authors.forename: Shicheng
authors.initials: S
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Genetic Engineering, Collaborative Innovation Center for Genetics and Development, School of Life Sciences, Fudan University, Shanghai 200433, China.
authors.forename: Jiucun
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Cardiothoracic Surgery, Huashan Hospital, Fudan University, Shanghai 200032, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Chen
mesh_terms: D017766:Acid Anhydride Hydrolases / Q000235:genetics*; D000368:Aged; D044466:Asian People / Q000235:genetics; D014408:Biomarkers, Tumor / Q000235:genetics*; D002289:Carcinoma, Non-Small-Cell Lung / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D016022:Case-Control Studies; D018899:CpG Islands; D019175:DNA Methylation; D030541:Databases, Genetic; D005260:Female; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000208:ethnology / Q000235:genetics* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000235:genetics*; D016017:Odds Ratio; D010641:Phenotype; D011237:Predictive Value of Tests; D011401:Promoter Regions, Genetic; D015203:Reproducibility of Results; D018570:Risk Assessment; D012307:Risk Factors; D044465:White People / Q000235:genetics; D000099170:Bis(5'-adenosyl)triphosphatase
chemical_list: D017766:Acid Anhydride Hydrolases; D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins; D000099170:Bis(5'-adenosyl)triphosphatase
keywords: DNA methylation; FHIT; NSCLC; diagnosis; non-small cell lung cancer