title: PER-8, a Novel Extended-Spectrum β-Lactamase PER Variant, from an Acinetobacter baumannii Clinical Isolate in Nepal.
abstract: A novel PER-type extended-spectrum β-lactamase, PER-8, was identified in anAcinetobacter baumanniiclinical isolate obtained in Nepal. The amino acid sequence of PER-8 has a substitution at position 39 (Gly to Glu) compared with that of PER-7. Thekcat/Kmratio of PER-8 for aztreonam was lower than that of PER-7, while thekcat/Kmratio of PER-8 for imipenem was higher than that of PER-7. The genomic environment surroundingblaPER-8wasintI1 blaPSE-1qacEDI sulIISCR1-blaPER-8gts sulI orfXon a 100-kb plasmid.
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Tada
authors.affiliation: Department of Medical Microbiology, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
authors.forename: Shovita
authors.initials: S
authors.lastname: Shrestha
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan.
authors.forename: Kayo
authors.initials: K
authors.lastname: Shimada
authors.affiliation: Department of International Medical-Cooperation, National Center for Global Health and Medicine, Tokyo, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ohara
authors.affiliation: Department of Medical Microbiology, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
authors.forename: Jeevan B
authors.initials: JB
authors.lastname: Sherchand
authors.affiliation: Department of Microbiology, Institute of Medicine, Tribhuvan University, Maharajgunj, Kathmandu, Nepal.
authors.forename: Bharat M
authors.initials: BM
authors.lastname: Pokhrel
authors.affiliation: Department of Infectious Diseases, Research Institute, National Center for Global Health and Medicine, Tokyo, Japan tkirikae@ri.ncgm.go.jp.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kirikae
mesh_terms: D000151:Acinetobacter Infections / Q000453:epidemiology / Q000382:microbiology; D040981:Acinetobacter baumannii / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000302:isolation & purification; D000595:Amino Acid Sequence; D019943:Amino Acid Substitution; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001398:Aztreonam / Q000494:pharmacology; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D006801:Humans; D015378:Imipenem / Q000494:pharmacology; D007700:Kinetics; D008826:Microbial Sensitivity Tests; D009390:Nepal / Q000453:epidemiology; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D015378:Imipenem; D001618:beta-Lactamases; D001398:Aztreonam
keywords: Acinetobacter baumannii; PER-type ESBLs; plasmid-mediated resistance