title: Identifying the cellular location of brain cytoplasmic 200 RNA using an RNA-recognizing antibody.
abstract: Brain cytoplasmic 200 RNA (BC200 RNA) is a neuron-specific non-coding RNA, implicated in the inhibition of local synaptodendritic protein synthesis, and is highly expressed in some cancer cells. Although BC200 RNA has been shown to inhibit translation in vitro, the cellular location of this inhibition is unknown. In this study, we used a BC200 RNA-recognizing antibody to identify the cellular locations of BC200 RNA in HeLa cervical carcinoma cells. We observed punctate signals in both the cytoplasm and nucleus, and further discovered that BC200 RNA co-localized with the p-body decapping enzyme, DCP1A, and the heterogeneous nuclear ribonucleoprotein E2 (hnRNP E2). The latter is a known BC200 RNA-binding partner protein and a constituent of p-bodies. This suggests that BC200 RNA is localized to p-bodies via hnRNP E2. [BMB Reports 2017; 50(6): 318-322].
authors.affiliation: Department of Chemistry, KAIST, Daejeon 34141, Korea.
authors.forename: Heegwon
authors.initials: H
authors.lastname: Shin
authors.affiliation: Department of Chemistry, KAIST, Daejeon 34141, Korea.
authors.forename: Jungmin
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Chemistry, KAIST, Daejeon 34141, Korea.
authors.forename: Youngmi
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Chemistry, KAIST, Daejeon 34141, Korea.
authors.forename: Seonghui
authors.initials: S
authors.lastname: Jang
authors.affiliation: Department of Cellular and Molecular Medicine, Chosun University School of Medicine, Gwangju 61452, Korea.
authors.forename: Takbum
authors.initials: T
authors.lastname: Ohn
authors.affiliation: Department of Chemistry, KAIST, Daejeon 34141, Korea.
authors.forename: Younghoon
authors.initials: Y
authors.lastname: Lee
mesh_terms: D001921:Brain / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism*; D003593:Cytoplasm / Q000378:metabolism*; D004722:Endoribonucleases / Q000235:genetics / Q000378:metabolism; D005260:Female; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000235:genetics; D012313:RNA / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism; D002583:Uterine Cervical Neoplasms / Q000235:genetics / Q000378:metabolism
chemical_list: D062085:RNA, Long Noncoding; D016601:RNA-Binding Proteins; D015534:Trans-Activators; C000609640:long non-coding RNA BC200, human; D012313:RNA; D004722:Endoribonucleases; C466279:DCP1A protein, human