title: Cooperative antioxidative effects of zein hydrolysates with sage (Salvia officinalis) extract in a liposome system.
abstract: This study investigated the cooperative antioxidative effects of sage extract (SE) and zein hydrolysates (ZH). The combination of 3mg/ml ZH and 10μg/ml SE exhibited a significant synergism in inhibition of the formation of thiobarbituric acid-reactive substances and provided superior protection of liposomes against oxidation. Zeta-potential results revealed that the interactions between liposomes and ZH were electrostatic interactions. Particle size determination further proved that ZH and SE added to oxidized liposomes significantly decreased the mean particle size. Confocal laser scanning microscopy revealed that when ZH was present in the liposome oxidizing system, the droplet sizes were obviously decreased compared to oxidized samples. ZH dispersed more uniformly and the interfacial membrane was more compact in the ZH-SE liposome. Transmission electron microscopy conveyed that the ZH-SE complex around the liposome particles could form a denser network structure, preventing radicals and oxidants from the approach of the liposomes.
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Haotian
authors.initials: H
authors.lastname: Liu
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Han
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address: kongbh63@hotmail.com.
authors.forename: Baohua
authors.initials: B
authors.lastname: Kong
authors.affiliation: College of Food Science, Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic address: liuqian_neau@hotmail.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry* / Q000648:ultrastructure; D010936:Plant Extracts / Q000494:pharmacology*; D027543:Salvia officinalis; D015028:Zein / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D008055:Lipids; D008081:Liposomes; D010936:Plant Extracts; D015028:Zein
keywords: Cooperative effects; Lipid oxidation; Liposome system; Sage extract; Zein hydrolysate