title: Effect of Wenhua Juanbi Recipe () on expression of receptor activator of nuclear factor kappa B ligand, osteoprotegerin, and tumor necrosis factor receptor superfamily member 14 in rats with collagen-induced arthritis.
abstract: OBJECTIVE
  To study the effect of Wenhua Juanbi Recipe (, WJR) on expression of receptor activator of nuclear factor kappa B ligand (RANKL), osteoprotegerin (OPG), and tumor necrosis factor receptor superfamily member 14 (TNFRSF14, also known as LIGHT) in rats with collagen-induced arthritis (CIA).
  
  
  METHODS
  CIA rats were generated by subcutaneous injection of bovine collagen type-II at the tail base. Sixty CIA rats were randomly assigned (10 animals/group) to: model, methotrexate (MTX)-treated (0.78 mg/kg body weight), and WJR-treated (22.9 g/kg) groups. Healthy normal rats (n=10) were used as the normal control. Treatments or saline were administered once daily by oral gavage. Rats were sacrifificed at day 28 post-treatment and knee synovium and peripheral blood serum were collected. Toe swelling degree and expression of RANKL, OPG, and LIGHT were determined by Western blot and immunohistochemistry.
  
  
  RESULTS
  Compared with the normal group, toe swelling degree was signifificantly increased in the model group (P<0.01). After treatment, toe swelling degree decreased signifificantly in the WJR and MTX groups compared with the model group (P<0.01). Compared with the normal group, expression of RANKL and LIGHT were signifificantly increased and OPG signifificantly decreased in peripheral blood and synovium of the model group (P<0.01). Conversely, RANKL and LIGHT expression were signifificantly reduced and OPG increased in the WJR and MTX groups compared with the model group (P<0.01). No statistically significant difference existed between WJR and MTX groups.
  
  
  CONCLUSIONS
  WJR likely acts by reducing RANKL expression and increasing OPG expression, thus inhibiting RANKL/RANK interaction and reducing LIGHT expression, thereby inhibiting osteoclast formation/activation to block bone erosion.
authors.affiliation: Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine, Hangzhou, 310003, China. liuxide2001@sohu.com.
authors.forename: Xi-de
authors.initials: XD
authors.lastname: Liu
authors.affiliation: Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310003, China.
authors.forename: Yun-Qing
authors.initials: YQ
authors.lastname: Wang
authors.affiliation: Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine, Hangzhou, 310003, China.
authors.forename: Long
authors.initials: L
authors.lastname: Cai
authors.affiliation: Department of Arthropathy, Zhejiang Provincial Hospital of Integrated Traditional and Western Medicine, Hangzhou, 310003, China.
authors.forename: Li-Hong
authors.initials: LH
authors.lastname: Ye
authors.affiliation: Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310003, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Zhejiang University of Traditional Chinese Medicine, Hangzhou, 310003, China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Feng
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy* / Q000378:metabolism*; D015153:Blotting, Western; D002417:Cattle; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D007150:Immunohistochemistry; D008297:Male; D053244:Osteoprotegerin / Q000378:metabolism*; D053245:RANK Ligand / Q000378:metabolism*; D017208:Rats, Wistar; D053240:Receptors, Tumor Necrosis Factor, Member 14 / Q000378:metabolism*; D013583:Synovial Membrane / Q000187:drug effects / Q000473:pathology
chemical_list: D004365:Drugs, Chinese Herbal; D053244:Osteoprotegerin; D053245:RANK Ligand; D053240:Receptors, Tumor Necrosis Factor, Member 14; C588019:wenhua juanbi
keywords: Chinese medicine; Wenhua Juanbi Recipe; collagen-induced arthritis; osteoprotegerin; peripheral blood; receptor activator of nuclear factor kappa B ligand; synovium; tumor necrosis factor receptor superfamily member 14