title: Photodynamic therapy and imaging based on tumor-targeted nanoprobe, polymer-conjugated zinc protoporphyrin.
abstract: OBJECTIVE
  To evaluate the potential of tumor-targeted nanoprobe,N-(2-hydroxypropyl)methacrylamide copolymer-conjugated zinc protoporphyrin (PZP) for photodynamic therapy (PDT) and tumor imaging.
  
  
  METHODS
  Different tumor models including carcinogen-induced cancer were used, PZP was intravenously injected followed by irradiation with xenon or blue fluorescent light on tumor.
  
  
  RESULTS
  One PZP 20 mg/kg (ZnPP equivalent) dose with two or three treatments of light at an intensity of ≥20 J/cm2caused necrosis and disappearance of most tumors (>70%) in different tumor models. We also confirmed PZP-based tumor imaging in carcinogen-induced breast tumor and colon cancer models.
  
  
  CONCLUSIONS
  These findings support the potential application of PZP as a tumor-selective nanoprobe for PDT as well as tumor imaging, by virtue of the enhanced permeability and retention effect.
authors.affiliation: Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Laboratory of Microbiology & Oncology, Faculty of Pharmaceutical Science, Sojo University, Kumamoto, Japan; Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Laboratory of Microbiology & Oncology, Faculty of Pharmaceutical Science, Sojo University, Kumamoto, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Fang
authors.affiliation: Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan.
authors.forename: Long
authors.initials: L
authors.lastname: Liao
authors.affiliation: Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Department of General Surgery, Sheng Jing Hospital, China Medical University, Shenyang City, Liaoning Province, 110004, P. R. China; Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Department of General Surgery, Sheng Jing Hospital, China Medical University, Shenyang City, Liaoning Province, 110004, P. R. China.
authors.forename: Hongzhuan
authors.initials: H
authors.lastname: Yin
authors.affiliation: Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Laboratory of Microbiology & Oncology, Faculty of Pharmaceutical Science, Sojo University, Kumamoto, Japan; Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Laboratory of Microbiology & Oncology, Faculty of Pharmaceutical Science, Sojo University, Kumamoto, Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Nakamura
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Subr
authors.affiliation: Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic; Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
authors.forename: Karel
authors.initials: K
authors.lastname: Ulbrich
authors.affiliation: Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan; Research Institute for Drug Delivery Science, Sojo University, Ikeda 4-22-1, Kumamoto 860-0082, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maeda
keywords: fluorescent nanoprobe; photodynamic therapy; theranostic nanomedicine; tumor imaging; zinc protoporphyrin