title: MicroRNA‑152 inhibits cell proliferation, migration and invasion by directly targeting MAFB in nasopharyngeal carcinoma.
abstract: Aberrant expression of microRNAs (miRs) has been reported to be involved in nasopharyngeal carcinoma (NPC) carcinogenesis and development. The expression and functions of miR‑152 have previously been studied in several types of cancer. However, to the best of our knowledge, no previous studies have investigated the effects of miR‑152 on NPC. The present study aimed to explore the expression, functions and molecular mechanisms of miR‑152 in NPC. The expression levels of miR‑152 were detected in NPC tissues and cell lines using quantitative polymerase chain reaction (qPCR). Cell proliferation, migration and invasion were measured by MTT, cell migration and invasion assays, respectively. Dual‑luciferase reporter assay was used to determine whether V‑maf avian musculoaponeurotic fibrosarcoma oncogene homolog B (MAFB) was a direct target gene of miR‑152. qPCR and western blotting were used to detect the mRNA and protein expression levels of MAFB. In addition, functional assays were performed to explore the effects of endogenous MAFB on NPC. The results of the present study demonstrated that miR‑152 was significantly downregulated in NPC tissues and cell lines. Furthermore, ectopic expression of miR‑152 suppressed cell proliferation, migration and invasion of NPC cells. Dual‑luciferase reporter assay demonstrated that MAFB was a direct target gene of miR‑152, and qPCR and western blotting indicated that miR‑152 negatively regulated MAFB expression at the mRNA and protein level. Knockdown of MAFB expression markedly suppressed NPC cell proliferation, migration and invasion. These findings suggested that miR‑152 may target MAFB to regulate NPC initiation and progression; therefore, it may be investigated as a target for the treatment of NPC.
authors.affiliation: Department of Otolaryngology, Affiliated Sixth People's Hospital of Shanghai Jiao Tong University, Shanghai 200233, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Otolaryngology Head Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Daliu
authors.initials: D
authors.lastname: Min
authors.affiliation: Department of Otolaryngology, Zhabei Central Hospital, Shanghai 200070, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Affiliated Sixth People's Hospital of Shanghai Jiao Tong University, Shanghai 200233, P.R. China.
authors.forename: Shankai
authors.initials: S
authors.lastname: Yin
authors.affiliation: Department of Otorhinolaryngology Head and Neck Surgery, Changhai Hospital, Second Military Medical University, Beijing 200433, P.R. China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Department of Otolaryngology Head Neck Surgery, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, P.R. China.
authors.forename: Liangfa
authors.initials: L
authors.lastname: Liu
mesh_terms: D000293:Adolescent; D000328:Adult; D002277:Carcinoma; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D051258:MafB Transcription Factor / Q000235:genetics*; D008297:Male; D035683:MicroRNAs / Q000235:genetics*; D008875:Middle Aged; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics* / Q000473:pathology*; D009305:Nasopharynx / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000235:genetics / Q000473:pathology; D055815:Young Adult
chemical_list: C490407:MAFB protein, human; C550856:MIRN152 microRNA, human; D051258:MafB Transcription Factor; D035683:MicroRNAs