title: Sperm DNA Fragmentation Index and Hyaluronan Binding Ability in Men from Infertile Couples and Men with Testicular Germ Cell Tumor.
abstract: Objective. To investigate sperm DNA fragmentation and sperm functional maturity in men from infertile couples (IC) and men with testicular germ cell tumor (TGCT).Materials and Methods. Semen samples were collected from 312 IC men and 23 men with TGCT before unilateral orchiectomy and oncological treatment. The sperm chromatin dispersion test was performed to determine DNA fragmentation index (DFI) and the ability of sperm to bind with hyaluronan (HA) was assessed.Results. In comparison with the IC men, the men with TGCT had a higher percentage of sperm with fragmented DNA (median 28% versus 21%;p< 0.01) and a lower percentage of HA-bound sperm (24% versus 66%;p< 0.001). Normal results of both analyses were observed in 24% of IC men and 4% of men with TGCT. Negative Spearman's correlations were found between DFI and the percentage of HA-bound sperm in the whole group and in IC subjects and those with TGCT analyzed separately.Conclusions. Approximately 76% of IC men and 96% with TGCT awaiting orchiectomy demonstrated DNA fragmentation and/or sperm immaturity. We therefore recommend sperm banking after unilateral orchiectomy, but before irradiation and chemotherapy; the use of such a deposit appears to be a better strategy to obtain functionally efficient sperms.
authors.affiliation: Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland.
authors.forename: Katarzyna
authors.identifier: 0000-0002-1533-4666
authors.initials: K
authors.lastname: Marchlewska
authors.affiliation: Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland.
authors.forename: Eliza
authors.initials: E
authors.lastname: Filipiak
authors.affiliation: Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Walczak-Jedrzejowska
authors.affiliation: II Clinic of Urology, Medical University of Lodz, Lodz, Poland.
authors.forename: Elzbieta
authors.initials: E
authors.lastname: Oszukowska
authors.affiliation: Infertility Clinic, Salve Medica, Lodz, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Sobkiewicz
authors.affiliation: Infertility Clinic, Salve Medica, Lodz, Poland.
authors.forename: Malgorzata
authors.initials: M
authors.lastname: Wojt
authors.affiliation: Infertility Clinic, Salve Medica, Lodz, Poland.
authors.forename: Jacek
authors.initials: J
authors.lastname: Chmiel
authors.affiliation: Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kula
authors.affiliation: Department of Andrology and Reproductive Endocrinology, Medical University of Lodz, Lodz, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Slowikowska-Hilczer
mesh_terms: D000328:Adult; D053938:DNA Fragmentation; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009373:Neoplasms, Germ Cell and Embryonal / Q000378:metabolism* / Q000473:pathology; D013094:Spermatozoa / Q000378:metabolism* / Q000473:pathology; D013736:Testicular Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: D006820:Hyaluronic Acid