title: Proteomic characterization of the outer membrane vesicle of the halophilic marine bacterium Novosphingobium pentaromativorans US6-1.
abstract: Novosphingobium pentaromativorans US6-1 is a Gram-negative halophilic marine bacterium able to utilize several polycyclic aromatic hydrocarbons such as phenanthrene, pyrene, and benzo[a]pyrene. In this study, using transmission electron microscopy, we confirmed that N. pentaromativorans US6-1 produces outer membrane vesicles (OMVs). N. pentaromativorans OMVs (hereafter OMVNovo) are spherical in shape, and the average diameter of OMVNovois 25-70 nm. Proteomic analysis revealed that outer membrane proteins and periplasmic proteins of N. pentaromativorans are the major protein components of OMVNovo. Comparative proteomic analysis with the membrane-associated protein fraction and correlation analysis demonstrated that the outer membrane proteins of OMVNovooriginated from the membrane- associated protein fraction. To the best of our knowledge, this study is the first to characterize OMV purified from halophilic marine bacteria.
authors.affiliation: Drug and Disease Target Team, Korea Basic Science Institute, Daejeon, 34133, Republic of Korea.
authors.forename: Sung Ho
authors.initials: SH
authors.lastname: Yun
authors.affiliation: Drug and Disease Target Team, Korea Basic Science Institute, Daejeon, 34133, Republic of Korea.
authors.forename: Sang-Yeop
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Tunneling Nanotube Research Center, Division of Life Science, Korea University, Seoul, 02841, Republic of Korea.
authors.forename: Chi-Won
authors.initials: CW
authors.lastname: Choi
authors.affiliation: Department of Bio-Analytical Science, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea.
authors.forename: Hayoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Drug and Disease Target Team, Korea Basic Science Institute, Daejeon, 34133, Republic of Korea.
authors.forename: Hyun-Joo
authors.initials: HJ
authors.lastname: Ro
authors.affiliation: Drug and Disease Target Team, Korea Basic Science Institute, Daejeon, 34133, Republic of Korea.
authors.forename: Sangmi
authors.initials: S
authors.lastname: Jun
authors.affiliation: National Marine Biodiversity Institute of Korea, Seocheon, 33662, Republic of Korea.
authors.forename: Yong Min
authors.initials: YM
authors.lastname: Kwon
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, 15627, Republic of Korea.
authors.forename: Kae Kyoung
authors.initials: KK
authors.lastname: Kwon
authors.affiliation: Korea Institute of Ocean Science and Technology, Ansan, 15627, Republic of Korea.|National Marine Biodiversity Institute of Korea, Seocheon, 33662, Republic of Korea.
authors.forename: Sang-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Drug and Disease Target Team, Korea Basic Science Institute, Daejeon, 34133, Republic of Korea.
authors.forename: Gun-Hwa
authors.initials: GH
authors.lastname: Kim
authors.affiliation: Drug and Disease Target Team, Korea Basic Science Institute, Daejeon, 34133, Republic of Korea. ksi@kbsi.re.kr.|Department of Bio-Analytical Science, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea. ksi@kbsi.re.kr.
authors.forename: Seung Il
authors.initials: SI
authors.lastname: Kim
mesh_terms: D001425:Bacterial Outer Membrane Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism*; D000067128:Extracellular Vesicles / Q000737:chemistry / Q000502:physiology* / Q000648:ultrastructure; D033903:Periplasmic Proteins / Q000378:metabolism; D021381:Protein Transport; D020543:Proteome; D040901:Proteomics; D012623:Seawater / Q000382:microbiology; D042301:Sphingomonadaceae / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D001425:Bacterial Outer Membrane Proteins; D033903:Periplasmic Proteins; D020543:Proteome
keywords: Novosphingobium pentaromativorans; outer membrane vesicle; proteomics