title: Expression profile of rrbp1 genes during embryonic development and in adult tissues of Xenopus laevis.
abstract: Recent studies suggest that ribosome-binding protein 1 (RRBP1) is involved in multiple diseases such as tumorigenesis and cardiomyopathies. However, its function during embryonic development remains largely unknown. We searched Xenopus laevis database with human RRBP1 protein sequence and identified two cDNA sequences encoding Xenopus orthologs of RRBP1 including rrbp1a (NM_001089623) and rrbp1b (NM_001092468). Both genes were firstly detected at blastula stage 8 with weak signals in animal hemisphere by whole mount in situ hybridization. Evident expression of rrbp1 was mainly detected in cement gland and notochord at neurula and tailbud stages. Heart expression of rrbp1 was detected at stage 36. RT-PCR results indicated that very weak expression of rrbp1a was firstly detected in oocytes, followed by increasing expression until stage 39. Differently, very weak expression of rrbp1b was firstly observed at stage 2, and then maintained at a lower level to stage 17 followed by an intense expression from stages 19-39. Moreover, both expression profiles were also different in adult tissues. This study reports Xenopus rrbp1 expression during early embryonic development and in adult tissues. Our study will facilitate the functional analysis of Rrbp1 family during embryonic development.
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou 510632, China.
authors.forename: Guang-Hui
authors.initials: GH
authors.lastname: Liu
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou 510632, China.
authors.forename: Cheng-Zhou
authors.initials: CZ
authors.lastname: Mao
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou 510632, China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Wu
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou 510632, China.
authors.forename: Deng-Cheng
authors.initials: DC
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou 510632, China.
authors.forename: Jing-Bo
authors.initials: JB
authors.lastname: Xia
authors.affiliation: Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 220-701, South Korea.
authors.forename: Soo-Ki
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou 510632, China. Electronic address: tdongbme@jnu.edu.cn.
authors.forename: Dong-Qing
authors.initials: DQ
authors.lastname: Cai
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China. Electronic address: zhaohui@cuhk.edu.hk.
authors.forename: Hui
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Regenerative Medicine, Ministry of Education, Department of Developmental & Regenerative Biology, Jinan University, Guangzhou 510632, China. Electronic address: qixufeng@jnu.edu.cn.
authors.forename: Xu-Feng
authors.initials: XF
authors.lastname: Qi
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D047108:Embryonic Development; D004721:Endoplasmic Reticulum / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D009206:Myocardium / Q000378:metabolism; D059467:Transcriptome; D029867:Xenopus Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014982:Xenopus laevis / Q000196:embryology* / Q000235:genetics / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; C000602809:RRBP1 protein, human; D029867:Xenopus Proteins; C000617331:ribosome-binding protein 1, Xenopus
keywords: Development; Expression; Xenopus laevis; rrbp1