title: Variation in Mitochondrial Cytochrome c Oxidase I DNA Can Successfully Identify Culex (Melanoconion) pedroi (Diptera: Culicidae) and Culex (Melanoconion) ribeirensis (Diptera: Culicidae).
abstract: Culex (Melanoconion) pedroiSirivanakarn & Belkin 1980 and Culex (Melanoconion) ribeirensisForattini & Sallum 1985 are two morphologically very similar species of the Pedroi subgroup of mosquitoes in the Spissipes section of the subgenus Melanoconion of the genus Culex L. 1758. We carried out an analysis of the mitochondrial cytochrome c oxidase I (COI) DNA variation between these two species. The recent observation of sympatric coexistence in a forested area of Rio de Janeiro State (Brazil) triggered the need to validate these two species previously identified based on morphology. We concluded that the COI is a useful tool for identification of Cx. pedroi and Cx. ribeirensis.
authors.affiliation: Laboratório de Biologia Molecular de Insetos, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil (saoriaraki13@gmail.com).
authors.forename: A S
authors.initials: AS
authors.lastname: Araki
authors.affiliation: Laboratório de Diptera, Instituto Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ, 21040-360, Brazil (dani.aguiar.maia@gmail.com; helciogil@ioc.fiocruz.br; ceciliafmello@gmail.com; jalencar@ioc.fiocruz.br).
authors.forename: D A
authors.initials: DA
authors.lastname: Maia
authors.affiliation: Laboratório de Diptera, Instituto Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ, 21040-360, Brazil (dani.aguiar.maia@gmail.com; helciogil@ioc.fiocruz.br; ceciliafmello@gmail.com; jalencar@ioc.fiocruz.br).
authors.forename: H R
authors.initials: HR
authors.lastname: Gil-Santana
authors.affiliation: Laboratório de Diptera, Instituto Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ, 21040-360, Brazil (dani.aguiar.maia@gmail.com; helciogil@ioc.fiocruz.br; ceciliafmello@gmail.com; jalencar@ioc.fiocruz.br ).|Programa de Pós-graduação em Biologia Animal, Universidade Federal Rural do Rio de Janeiro, Brazil.
authors.forename: C F
authors.initials: CF
authors.lastname: de Mello
authors.affiliation: Laboratório de Fisiologia e Controle de Artrópodes Vetores, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil (ademirjr@ioc.fiocruz.br).
authors.forename: A de J
authors.initials: AJ
authors.lastname: Martins
authors.affiliation: Laboratório de Diptera, Instituto Oswaldo Cruz, Av. Brasil, 4365, Rio de Janeiro, RJ, 21040-360, Brazil (dani.aguiar.maia@gmail.com; helciogil@ioc.fiocruz.br; ceciliafmello@gmail.com; jalencar@ioc.fiocruz.br).
authors.forename: J
authors.initials: J
authors.lastname: Alencar
mesh_terms: D000818:Animals; D001938:Brazil; D003465:Culex / Q000145:classification* / Q000201:enzymology / Q000235:genetics; D004272:DNA, Mitochondrial / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D019476:Insect Proteins / Q000235:genetics*; D008928:Mitochondria / Q000201:enzymology / Q000235:genetics*
chemical_list: D004272:DNA, Mitochondrial; D019476:Insect Proteins; D003576:Electron Transport Complex IV
keywords: Culex pedroi, Culex ribeirensis; cytochrome c oxidase I; phylogeny; taxonomy