title: MMP-1 and MMP-8 expression in giant-cell fibroma and inflammatory fibrous hyperplasia.
abstract: The aim of this study is to compare the immunoexpression of metalloproteinases 1 and 8 in giant-cell fibroma, inflammatory fibrous hyperplasia and normal mucosa. Twenty-two cases of giant-cell fibroma, inflammatory fibrous hyperplasia and oral mucosa (control) each were subjected to immunohistochemistry using anti-metalloproteinase-1 and anti-metalloproteinase-8 antibodies. Eight images of each case were captured and analysed through the a) application of a count grid to count the number of positive neutrophils, macrophages, lymphocytes, plasma cells, fibroblasts and blood vessels to obtain the percentage of staining and b) semi-automated segmentation quantifying the stained area in square micrometres. Statistical tests included ANOVA Two-way, Kruskal Wallis and Games-Howell, with a significance level of 5%. An increased percentage of metalloproteinase-1-immunopositive blood vessels were observed in giant-cell fibroma (26.6±22.4; p=0.02) and inflammatory fibrous hyperplasia (34.3±31.5; p=0.01) compared with the control group (19.6±9.2). No significant differences in inflammatory cells, fibroblasts and total area of metalloproteinase-1 and -8 were noted among the three groups. Metalloproteinase-1 apparently acts within the pathogenesis of giant-cell fibroma and inflammatory fibrous hyperplasia.
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: climeck@live.com.
authors.forename: Henrique Climeck
authors.initials: HC
authors.lastname: de Oliveira
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: andre.tschoeke@hotmail.com.
authors.forename: André
authors.initials: A
authors.lastname: Tschoeke
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: gabe-cruz@hotmail.com.
authors.forename: Gabriele Claudino
authors.initials: GC
authors.lastname: da Cruz
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: lnno@terra.com.br.
authors.forename: Lúcia
authors.initials: L
authors.lastname: Noronha
authors.affiliation: Universidade Positivo, Department of Anatomy, Surgery and Bucomaxillofacial Traumatology, Prof. Pedro Viriato Parigot de Souza, 5300 - Cidade Industrial, Curitiba, PR, 81280-330, Brazil. Electronic address: rafaela_scariot@yahoo.com.br.
authors.forename: Rafaela Scariot
authors.initials: RS
authors.lastname: de Moraes
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6667 sala 3202-D, Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: ramesquita@ufmg.br.
authors.forename: Ricardo Alves
authors.initials: RA
authors.lastname: Mesquita
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6667 sala 3202-D, Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: cassiafaster@gmail.com.
authors.forename: Maria Cássia Ferreira
authors.initials: MC
authors.lastname: de Aguiar
authors.affiliation: Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6667 sala 3202-D, Pampulha, 31.270-901, Belo Horizonte, MG, Brazil. Electronic address: pat_caldeira@yahoo.com.br.
authors.forename: Patrícia Carlos
authors.initials: PC
authors.lastname: Caldeira
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: marina.ribas@pucpr.br.
authors.forename: Marina
authors.initials: M
authors.lastname: de Oliveira Ribas
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: ana.gregio@pucpr.br.
authors.forename: Ana Maria Trindade
authors.initials: AM
authors.lastname: Grégio
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: lrazevedo@yahoo.com.
authors.forename: Luciana Reis Azevedo
authors.initials: LR
authors.lastname: Alanis
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: s.ignacio@pucpr.br.
authors.forename: Sérgio Aparecido
authors.initials: SA
authors.lastname: Ignácio
authors.affiliation: Department of Oral Pathology, School of Dentistry, Universidade Federal da Bahia, Av. Araújo Pinho, 62, Canela, 40110-150, Salvador, BA, Brazil. Electronic address: jeanpatol@gmail.com.
authors.forename: Jean Nunes
authors.initials: JN
authors.lastname: Dos Santos
authors.affiliation: Department of Stomatology, School of Dentistry, Universidade Federal do Paraná, Rua Prefeito Lothário Meissner 632 Campus II, Jardim Botânico, 80210170 - Curitiba, PR, Brazil. Electronic address: aas.lima@ufpr.br.
authors.forename: Antonio Adilson Soares
authors.initials: AA
authors.lastname: de Lima
authors.affiliation: Pontifícia Universidade Católica do Paraná, School of Health and Biosciences, Rua Imaculada Conceição, 1155, Prado Velho 80.215-901, Curitiba, PR, Brazil. Electronic address: aline.johann@pucpr.br.
authors.forename: Aline Cristina Batista Rodrigues
authors.initials: AC
authors.lastname: Johann
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis; D005350:Fibroma / Q000175:diagnosis*; D015726:Giant Cells / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000175:diagnosis; D007150:Immunohistochemistry; D020781:Matrix Metalloproteinase 1 / Q000032:analysis / Q000096:biosynthesis*; D020784:Matrix Metalloproteinase 8 / Q000032:analysis / Q000096:biosynthesis*
chemical_list: D014408:Biomarkers, Tumor; C488762:MMP8 protein, human; D020784:Matrix Metalloproteinase 8; C522360:MMP1 protein, human; D020781:Matrix Metalloproteinase 1
keywords: Collagen; Metalloproteinase-1; Metalloproteinase-8