title: Depth-resolved imaging of colon tumor using optical coherence tomography and fluorescence laminar optical tomography.
abstract: Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those of the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect the subsurface lesions. In this research, we investigated the feasibility of a novel multi-modal optical imaging approach including high-resolution optical coherence tomography (OCT) and high-sensitivity fluorescence laminar optical tomography (FLOT) for structural and molecular imaging. The C57BL/6J-ApcMin/J mice were imaged using OCT and FLOT, and the correlated histopathological diagnosis was obtained. Quantitative structural (scattering coefficient) and molecular (relative enzyme activity) parameters were obtained from OCT and FLOT images for multi-parametric analysis. This multi-modal imaging method has demonstrated the feasibility for more accurate diagnosis with 88.23% (82.35%) for sensitivity (specificity) compared to either modality alone. This study suggested that combining OCT and FLOT is promising for subsurface cancer detection, diagnosis, and characterization.
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
authors.forename: Qinggong
authors.initials: Q
authors.lastname: Tang
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
authors.forename: Jianting
authors.initials: J
authors.lastname: Wang
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
authors.forename: Aaron
authors.initials: A
authors.lastname: Frank
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Lin
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA; Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Zhifang
authors.initials: Z
authors.lastname: Li
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
authors.forename: Chao-Wei
authors.initials: CW
authors.lastname: Chen
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA.
authors.forename: Lily
authors.initials: L
authors.lastname: Jin
authors.affiliation: Department of Biostatistics and Computational Biology, University of Rochester, Rochester, NY 14642, USA.
authors.forename: Tongtong
authors.initials: T
authors.lastname: Wu
authors.affiliation: Division of Gastroenterology and Hepatology, University of Maryland School of Medicine, Baltimore, MD 21201, USA.
authors.forename: Bruce D
authors.initials: BD
authors.lastname: Greenwald
authors.affiliation: Department of Medicine, Veterans Affairs Boston Healthcare System, Harvard Medical School, West Roxbury, MA 02132, USA.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Mashimo
authors.affiliation: Fischell Department of Bioengineering, University of Maryland, College Park, MD 20742, USA; Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, College of Photonic and Electronic Engineering, Fujian Normal University, Fuzhou 350007, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
keywords: (110.4190) Multiple imaging; (170.2520) Fluorescence microscopy; (170.3880) Medical and biological imaging; (170.4500) Optical coherence tomography