title: High expression of the long non-coding RNA HEIRCC promotes Renal Cell Carcinoma metastasis by inducing epithelial-mesenchymal transition.
abstract: Increasing evidence indicates that long non-coding RNAs (lncRNAs) have been associated with cancer development. However, the contributions of lncRNAs to renal cell carcinoma (RCC) remain poorly characterized. Here, we identified a novel lncRNA, termed HEIRCC, which was up-regulated in RCC tissues through lncRNA microarray analysis and subsequent validation in 60 RCC clinical specimens and cell lines. The high expression of HEIRCC is associated closely with the clinical pathology features such as larger tumor size, poor differentiation, lymphatic metastasis. In vitro assays revealed that HEIRCC knockdown could inhibit cell proliferation, trigger late apoptosis, suppress cell migration and invasion. We further demonstrated that depletion of HEIRCC reduce the epithelial to mesenchymal transition (EMT) program by regulating expression levels of EMT-associated markers in RCC cells. Thus, HEIRCC might be act as an important regulator of EMT in RCC progression and might be a novel therapeutic target for the advanced RCC therapy.
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.|Department of Urology and Andrology, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei Province, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Preventive Medicine, School of Public Health and Management, Hubei University of Medicine, Shiyan, Hubei Province, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Shengjun
authors.initials: S
authors.lastname: Luo
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Li
authors.initials: L
authors.lastname: Jiang
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Zhixiong
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Xiaobo
authors.initials: X
authors.lastname: Shi
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Bufan
authors.initials: B
authors.lastname: Lv
authors.affiliation: Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing.
authors.forename: Wei
authors.initials: W
authors.lastname: Tang
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000378:metabolism* / Q000556:secondary; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D020411:Oligonucleotide Array Sequence Analysis; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D047368:Tumor Burden; D015854:Up-Regulation
chemical_list: D014408:Biomarkers, Tumor; D062085:RNA, Long Noncoding
keywords: EMT; HEIRCC; long non-coding RNAs; prognosis; renal cell carcinoma