title: Preparation of PEGylated polymeric nanoprobes with aggregation-induced emission feature through the combination of chain transfer free radical polymerization and multicomponent reaction: Self-assembly, characterization and biological imaging applications.
abstract: Self-assembly of amphiphilic luminescent copolymers is a general route to fabricate fluorescent polymeric microparticles (FPMs). In this work, the FPMs with aggregation-induced emission (AIE) feature were fabricated via the combination of the chain transfer free radical polymerization and "one-pot" multicomponent reaction, which conjugated the aldehyde-containing AIE active dye AIE (CHO-An-CHO) and amino-terminated hydrophilic polymer (ATPPEGMA) using mercaptoacetic acid (MTA) as the "lock" molecule. The structure, chemical compositions, optical properties as well as biological properties of the PPEGMA-An-PPEGMA FPMs were characterized and investigated by means of a series of techniques and experiments in detail. We demonstrated the final copolymers showed amphiphilic properties, strong yellow fluorescence and high water dispersibility. Biological evaluation suggested that PPEGMA-An-PPEGMA FPMs possess low cytotoxicity and can be used for cell imaging. More importantly, many other AIE active FPMs are expected to be fabricated using the similar strategy because of the good substrate and monomer applicability of the multicomponent reaction and chain transfer living radical polymerization. Therefore, we could conclude that the strategy described in this work should be of great interest for fabrication of multifunctional AIE active nanoprobes for biomedical applications.
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Wan
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Meiying
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Liucheng
authors.initials: L
authors.lastname: Mao
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Ruming
authors.initials: R
authors.lastname: Jiang
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Dazhuang
authors.initials: D
authors.lastname: Xu
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Hongye
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Yanfeng
authors.initials: Y
authors.lastname: Dai
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China.
authors.forename: Fengjie
authors.initials: F
authors.lastname: Deng
authors.affiliation: Department of Chemistry and Jiangxi Provincial Key Laboratory of New Energy Chemistry, Nanchang University, 999 Xuefu Avenue, Nanchang 330031, China. Electronic address: xiaoyongzhang1980@gmail.com.
authors.forename: Xiaoyong
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and the Tsinghua Center for Frontier Polymer Research, Tsinghua University, Beijing 100084, China.
authors.forename: Yen
authors.initials: Y
authors.lastname: Wei
mesh_terms: D002470:Cell Survival / Q000187:drug effects; D005609:Free Radicals / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008689:Methacrylates / Q000737:chemistry; D018613:Microscopy, Confocal; D046529:Microscopy, Electron, Transmission; D053758:Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry* / Q000633:toxicity; D011109:Polymethacrylic Acids; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D005609:Free Radicals; D008689:Methacrylates; D011108:Polymers; D011109:Polymethacrylic Acids; C528061:poly(poly(ethylene glycol)methacrylate); D011092:Polyethylene Glycols
keywords: Aggregation-induced emission; Biomedical imaging; Chain transfer free radical polymerization; Fluorescent polymeric nanoparticles; Multicomponent reaction