title: Microspore Induced Doubled Haploids Production from Ethyl Methanesulfonate (EMS) Soaked Flower Buds Is an Efficient Strategy for Mutagenesis in Chinese Cabbage.
abstract: Chinese cabbage buds were soaked with Ethyl methanesulfonate (EMS) to induce mutagenesis. The influence of different EMS concentrations and treatment durations on microspore development, embryo production rate and seedling rate were evaluated in five Chinese cabbage genotypes. Mutations in four color-related genes were identified using high resolution melting (HRM) curves of their PCR products. The greatest embryo production and seedling rates were observed when buds were treated with 0.03 to 0.1% EMS for 5 to 10 min, while EMS concentrations greater than 0.1% were lethal to the microspores. In total, 142 mutants with distinct variations in leaf shape, leaf color, corolla size, flower color, bolting time and downy mildew resistance were identified from 475 microspore culture derived Doubled Haploids. Our results demonstrate that microspore derived Doubled Haploids from EMS soaked buds represents an efficient approach to rapidly generate homozygous Chinese cabbage mutants.
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Shuangyan
authors.initials: S
authors.lastname: Dai
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Aixia
authors.initials: A
authors.lastname: Gu
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Mengyang
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Shuangxia
authors.initials: S
authors.lastname: Luo
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Xuan
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Xueping
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Li
authors.affiliation: Plant Breeding, Wageningen University & Research Wageningen, Netherlands.
authors.forename: Guusje
authors.initials: G
authors.lastname: Bonnema
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Vegetable Germplasm and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Agricultural University of Hebei Baoding, China.
authors.forename: Shuxing
authors.initials: S
authors.lastname: Shen
keywords: Brassica rapa; Chinese cabbage; EMS mutagenesis; microspore culture; mutation