title: Inflammation Related MicroRNAs Are Modulated in Total Plasma and in Extracellular Vesicles from Rats with Chronic Ingestion of Sucrose.
abstract: Circulating microRNAs (miRNAs) and the functional implications of miRNAs contained in extracellular vesicles (EVs) have gained attention in the last decade. Little is known about the regulation of the abundance of plasma miRNAs in response to chronic ingestion of carbohydrates. Therefore, we explored the circulating levels of miR-21, miR-146a, miR-155, and miR-223 in rats consuming sucrose in drinking water. Weanling Wistar rats were 25 weeks with 30% sucrose in drinking water, and miRNAs expression was determined in total plasma and in microvesicles, by RT-qPCR with TaqMan probe based assays for miR-21, miR-146a, miR-155, and miR-223, using cel-miR-39 (as spike in control and reference). Endotoxemia was also measured. Sucrose-fed animals showed higher body weight and retroperitoneal adipose tissue as well as higher glucose and triglyceride plasma levels than controls. Plasma endotoxin levels were low and not different among groups. Plasma miR-21 and miR-223 were higher in the sucrose group (p< 0.05), whereas miR-155 tended to be lower (p= 0.0661), and miR-146a did not show significant differences. In the plasma EVs the same trend was found except for miR-146a that showed significantly higher levels (p< 0.05). Overall, our results show that high carbohydrate ingestion modulates circulating miRNAs levels related to an inflammatory response.
authors.affiliation: Posgrado en Biología Experimental, Universidad Autónoma Metropolitana-Iztapalapa, Av. San Rafael Atlixco No. 186, Col. Vicentina, Iztapalapa, 09340 Mexico City, Mexico.
authors.forename: Malinalli
authors.initials: M
authors.lastname: Brianza-Padilla
authors.affiliation: Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico.
authors.forename: Roxana
authors.initials: R
authors.lastname: Carbó
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico.
authors.forename: Julio C
authors.initials: JC
authors.lastname: Arana
authors.affiliation: Colegio de Ciencias y Humanidades, Universidad Autónoma de la Ciudad de México-San Lorenzo Tezonco, Av. Prolongación San Isidro No. 151, Col. San Lorenzo Tezonco, Iztapalapa, 09790 Mexico City, Mexico.
authors.forename: Gonzalo
authors.initials: G
authors.lastname: Vázquez-Palacios
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico.
authors.forename: Martha A
authors.initials: MA
authors.lastname: Ballinas-Verdugo
authors.affiliation: Departamento de Endocrinología, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico.
authors.forename: Guillermo C
authors.initials: GC
authors.lastname: Cardoso-Saldaña
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico.
authors.forename: Adán G
authors.initials: AG
authors.lastname: Palacio
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico.
authors.forename: Yaneli
authors.initials: Y
authors.lastname: Juárez-Vicuña
authors.affiliation: Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Xochimilco, Calzada del Hueso 1100, Villa Quietud, Coyoacan, 04960 Mexico City, Mexico.
authors.forename: Fausto
authors.initials: F
authors.lastname: Sánchez
authors.affiliation: Instituto Nacional de Medicina Genómica, Periférico Sur No. 4809, Col. Arenal Tepepan, Tlalpan, 14610 Mexico City, Mexico.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Martínez-Martínez
authors.affiliation: Laboratorio de Farmacología y Toxicología, Hospital Infantil de México Federico Gómez, Calle Dr. Márquez No. 162, Cuauhtemoc, Doctores, 06720 Ciudad de México, Mexico.
authors.forename: Fengyang
authors.initials: F
authors.lastname: Huang
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico.
authors.forename: Fausto
authors.identifier: 0000-0001-6556-1632
authors.initials: F
authors.lastname: Sánchez-Muñoz
authors.affiliation: Departamento de Inmunología, Instituto Nacional de Cardiología "Ignacio Chávez", Juan Badiano No. 1 Col. Sección XVI, Tlalpan, 14080 Mexico City, Mexico; Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana Xochimilco, Calzada del Hueso 1100, Villa Quietud, Coyoacan, 04960 Mexico City, Mexico.
authors.forename: Rafael
authors.identifier: 0000-0002-5806-3675
authors.initials: R
authors.lastname: Bojalil
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D055252:Cell-Derived Microparticles / Q000378:metabolism*; D018836:Inflammation Mediators / Q000097:blood*; D008297:Male; D035683:MicroRNAs / Q000097:blood*; D051381:Rats; D017208:Rats, Wistar; D013395:Sucrose / Q000494:pharmacology*; D014280:Triglycerides / Q000097:blood
chemical_list: D001786:Blood Glucose; D018836:Inflammation Mediators; D035683:MicroRNAs; D014280:Triglycerides; D013395:Sucrose