title: Endothelin Regulates Porphyromonas gingivalis-Induced Production of Inflammatory Cytokines.
abstract: Periodontitis is a very common oral inflammatory disease that results in the destruction of supporting connective and osseous tissues of the teeth. Although the exact etiology is still unclear, Gram-negative bacteria, especially Porphyromonas gingivalis in subgingival pockets are thought to be one of the major etiologic agents of periodontitis. Endothelin (ET) is a family of three 21-amino acid peptides, ET-1, -2, and -3, that activate G protein-coupled receptors, ETA and ETB. Endothelin is involved in the occurrence and progression of various inflammatory diseases. Previous reports have shown that ET-1 and its receptors, ETA and ETB are expressed in the periodontal tissues and, that ET-1 levels in gingival crevicular fluid are increased in periodontitis patients. Moreover, P. gingivalis infection has been shown to induce the production of ET-1 along with other inflammatory cytokines. Despite these studies, however, the functional significance of endothelin in periodontitis is still largely unknown. In this study, we explored the cellular and molecular mechanisms of ET-1 action in periodontitis using human gingival epithelial cells (HGECs). ET-1 and ETA, but not ETB, were abundantly expressed in HGECs. Stimulation of HGECs with P. gingivalis or P. gingivalis lipopolysaccharide increased the expression of ET-1 and ETA suggesting the activation of the endothelin signaling pathway. Production of inflammatory cytokines, IL-1β, TNFα, and IL-6, was significantly enhanced by exogenous ET-1 treatment, and this effect depended on the mitogen-activated protein kinases via intracellular Ca2+ increase, which resulted from the activation of the phospholipase C/inositol 1,4,5-trisphosphate pathway. The inhibition of the endothelin receptor-mediated signaling pathway with the dual receptor inhibitor, bosentan, partially ameliorated alveolar bone loss and immune cell infiltration. These results suggest that endothelin plays an important role in P. gingivalis-mediated periodontitis. Thus, endothelin antagonism may be a potential therapeutic approach for periodontitis treatment.
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Ga-Yeon
authors.initials: GY
authors.lastname: Son
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Bak
authors.affiliation: Department of Dental Hygiene, Jeonju Kijeon College, Jeonju, Republic of Korea.
authors.forename: Ji-Hye
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Dong Eun
authors.initials: DE
authors.lastname: Lee
authors.affiliation: BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.|Department of Preventive Dentistry and Public Oral Health, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Si-Mook
authors.initials: SM
authors.lastname: Kang
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: So Yun
authors.initials: SY
authors.lastname: Lee
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Lin
authors.initials: L
authors.lastname: Choi
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Ji Su
authors.initials: JS
authors.lastname: Sun
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Kim
authors.affiliation: Department of Advanced General Dentistry, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Wonse
authors.initials: W
authors.lastname: Park
authors.affiliation: BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.|Department of Preventive Dentistry and Public Oral Health, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Baek Il
authors.initials: BI
authors.lastname: Kim
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Yun-Jung
authors.initials: YJ
authors.lastname: Yoo
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Inik
authors.initials: I
authors.lastname: Chang
authors.affiliation: Department of Oral Biology, Yonsei University College of Dentistry, Seoul, Republic of Korea.|BK21 PLUS Project, Yonsei University College of Dentistry, Seoul, Republic of Korea.
authors.forename: Dong Min
authors.initials: DM
authors.lastname: Shin
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D016207:Cytokines / Q000378:metabolism*; D018450:Disease Progression; D019332:Endothelin-1 / Q000096:biosynthesis / Q000378:metabolism*; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000382:microbiology; D006801:Humans; D007249:Inflammation / Q000378:metabolism; D008297:Male; D051379:Mice; D010518:Periodontitis / Q000473:pathology; D016966:Porphyromonas gingivalis / Q000502:physiology*; D015398:Signal Transduction
chemical_list: D016207:Cytokines; D019332:Endothelin-1; D002118:Calcium