title: Decoupling Electronic versus Nuclear Photoresponse of Isolated Green Fluorescent Protein Chromophores Using Short Laser Pulses.
abstract: The photophysics of a deprotonated model chromophore for the green fluorescent protein is studied by femtosecond laser pulses in an electrostatic ion-storage ring. The laser-pulse duration is much shorter than the time for internal conversion, and, hence, contributions from sequential multiphoton absorption, typically encountered with ns-laser pulses, are avoided. Following single-photon excitation, the action-absorption maximum is shown to be shifted within the S_{0} to S_{1} band from its origin at about 490 to 450 nm, which is explained by the different photophysics involved in the detected action.
authors.affiliation: Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark.
authors.forename: Hjalte V
authors.initials: HV
authors.lastname: Kiefer
authors.affiliation: Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark.
authors.forename: Henrik B
authors.initials: HB
authors.lastname: Pedersen
authors.affiliation: Chemistry Department, Lomonosov Moscow State University, 119991 Moscow, Russia.
authors.forename: Anastasia V
authors.initials: AV
authors.lastname: Bochenkova
authors.affiliation: Department of Physics and Astronomy, Aarhus University, DK-8000 Aarhus C, Denmark.
authors.forename: Lars H
authors.initials: LH
authors.lastname: Andersen
mesh_terms: D049452:Green Fluorescent Proteins / Q000737:chemistry*; D007834:Lasers; D008027:Light; D017785:Photons; D055672:Static Electricity
chemical_list: D049452:Green Fluorescent Proteins