title: An RNA-binding atypical tropomyosin recruits kinesin-1 dynamically to oskar mRNPs.
abstract: Localization and local translation of oskar mRNA at the posterior pole of the Drosophila oocyte directs abdominal patterning and germline formation in the embryo. The process requires recruitment and precise regulation of motor proteins to form transport-competent mRNPs. We show that the posterior-targeting kinesin-1 is loaded upon nuclear export of oskar mRNPs, prior to their dynein-dependent transport from the nurse cells into the oocyte. We demonstrate that kinesin-1 recruitment requires the DmTropomyosin1-I/C isoform, an atypical RNA-binding tropomyosin that binds directly to dimerizing oskar 3'UTRs. Finally, we show that a small but dynamically changing subset of oskar mRNPs gets loaded with inactive kinesin-1 and that the motor is activated during mid-oogenesis by the functionalized spliced oskar RNA localization element. This inefficient, dynamic recruitment of Khc decoupled from cargo-dependent motor activation constitutes an optimized, coordinated mechanism of mRNP transport, by minimizing interference with other cargo-transport processes and between the cargo-associated dynein and kinesin-1.
authors.affiliation: Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany gaspar@embl.de ephrussi@embl.de.
authors.forename: Imre
authors.identifier: 0000-0002-6527-637X
authors.initials: I
authors.lastname: Gáspár
authors.affiliation: Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Vasiliy
authors.identifier: 0000-0002-8375-9105
authors.initials: V
authors.lastname: Sysoev
authors.affiliation: Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
authors.forename: Artem
authors.identifier: 0000-0003-0278-8308
authors.initials: A
authors.lastname: Komissarov
authors.affiliation: Developmental Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany gaspar@embl.de ephrussi@embl.de.
authors.forename: Anne
authors.identifier: 0000-0002-5061-4620
authors.initials: A
authors.lastname: Ephrussi
mesh_terms: D000818:Animals; D004330:Drosophila / Q000502:physiology*; D029721:Drosophila Proteins / Q000378:metabolism*; D016547:Kinesins / Q000378:metabolism*; D011485:Protein Binding; D021381:Protein Transport; D012261:Ribonucleoproteins / Q000378:metabolism*; D014335:Tropomyosin / Q000378:metabolism*
chemical_list: D029721:Drosophila Proteins; D012261:Ribonucleoproteins; C490776:Tm1 protein, Drosophila; D014335:Tropomyosin; C026452:messenger ribonucleoprotein; C091675:osk protein, Drosophila; C515209:Khc protein, Drosophila; D016547:Kinesins
keywords: RNA binding protein; active transport; atypical tropomyosin isoform; molecular motor; oocyte