title: [Effect of secretin on the expression of cPLA2and mPGEs-1 in mouse endometrial stromal cell during early pregnancy].
abstract: Secretin, a gastrointestinal peptide, has been found to be expressed in mouse endometrial stromal cells (mESCs) during early pregnancy. In order to further investigate the function of secretin during embryo implantation, the expression levels of secretin, secretin receptor, cytosolic phospholipase A2(cPLA2) and membrane prostaglandin E synthase 1 (mPGEs-1) were detected in the mice uterus from day 4 to 8 of pregnancy by real-time PCR, ELISA and in situ hybridization. mESCs isolated and cultured from day 4 of pregnancy were transfected with secretin expression vectors or treated with H89, a PKA inhibitor. Then the expression levels of cPLA2, mPGEs-1 and cAMP responsive element-binding protein (CREB) were detected by real-time PCR and Western blot. The concentration of prostaglandin E2 (PGE2) in the supernatant was determined by ELISA. The result showed that secretin, cPLA2and mPGEs-1 mRNA expression increased gradually in implantation sites from day 5 to day 7 of pregnancy with the same tendency. The secretin levels in serum were significantly higher on days 6, 7 and 8 of pregnancy than that on day 5 of pregnancy. The concentration of secretin was significantly higher in implantation sites on days 6, 7 than that in non-implantation site on day 5. Transfection of secretin expression vector promoted cPLA2, p-cPLA2and mPGEs-1 expressions in mESCs, but not PGE2level in the supernatant. H89 could effectively inhibit the expression of CREB, p-CREB, p-cPLA2and cPLA2induced by secretin. The results showed that the increased secretin expression in mESCs during embryo implantation may promote p-cPLA2, cPLA2and mPGEs-1 expression, and the promotion may be through PKA signaling pathway.
authors.affiliation: The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, Life Science College of Anqing Normal University, Anqing 246011, China. huangzhu@xmu.edu.cn.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Reproductive Medicine Center of Nanjing General Hospital, Nanjing 210002, China.
authors.forename: Yi-Feng
authors.initials: YF
authors.lastname: Ge
authors.affiliation: Reproductive Medicine Center of Nanjing General Hospital, Nanjing 210002, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jing
authors.affiliation: The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, Life Science College of Anqing Normal University, Anqing 246011, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wu
authors.affiliation: The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, Life Science College of Anqing Normal University, Anqing 246011, China.
authors.forename: Zheng-Yu
authors.initials: ZY
authors.lastname: Zhou
authors.affiliation: The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, Life Science College of Anqing Normal University, Anqing 246011, China.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Zhu
authors.affiliation: The Province Key Laboratory of the Biodiversity Study and Ecology Conservation in Southwest Anhui, Life Science College of Anqing Normal University, Anqing 246011, China.
authors.forename: Ting-Zhe
authors.initials: TZ
authors.lastname: Sun
mesh_terms: D000818:Animals; D015153:Blotting, Western; D017362:Cyclic AMP Response Element-Binding Protein; D015232:Dinoprostone; D005260:Female; D051379:Mice; D054510:Phospholipases A2, Cytosolic; D011247:Pregnancy; D000071876:Prostaglandin-E Synthases; D060888:Real-Time Polymerase Chain Reaction; D012633:Secretin; D017154:Stromal Cells; D014599:Uterus
chemical_list: D017362:Cyclic AMP Response Element-Binding Protein; D012633:Secretin; D054510:Phospholipases A2, Cytosolic; D000071876:Prostaglandin-E Synthases; D015232:Dinoprostone