title: Effects of 17β-estradiol on leptin signaling in anterior pituitary of ovariectomized rats.
abstract: Leptin is secreted predominantly by adipocytes and exerts its role mainly by interaction with the long form of leptin receptor (LEPR_V2). It has been identified that LEPR_V2 is widely distributed in various tissues, including the anterior pituitary. Cross-talk between leptin and estrogens has been indentified. Estrogen is known to modulate the tissue-specific expression of LEPR_V2 and leptin in ovariectomized (OVX) rats, a model of postmenopausal condition. Our previous data showed that 17β-estradiol (E2) up-regulated the expression of LEPR_V2 protein and mRNA in rat dorsal root ganglion (DRG) in an estrogen receptor alpha (ERα)-dependent manner. But it is still unclear whether estrogen can regulate leptin signalling in the pituitary of OVX rats. In the present study, we found that ovariectomy decreased the expressions of LEPR_V2. Administration of E2increased the expressions of LEPR_V2 in a dose-dependent manner. In addition, E2improved LEPR_V2, STAT3, and SOCS3 protein levels in OVX rats. The effects of exogenous E2were attenuated by ICI 182,780, a specific estrogen receptors antagonist. However, E2did not change the Lepr_v1, a type of short form of leptin receptor (LEPR), or leptin mRNA levels. Thus, E2plays a crucial role in regulating pituitary sensitivity to leptin in OVX rats. Our findings implied that exogenous E2had potential roles in modification of the function of pituitary in postmenopausal women.
authors.affiliation: Department of Gynecology and Obstetric, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Chunhua
authors.initials: C
authors.lastname: Yin
authors.affiliation: Department of Animal Science, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Lumei
authors.initials: L
authors.lastname: Kang
authors.affiliation: Department of Histology and Embryology, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Cong
authors.initials: C
authors.lastname: Lai
authors.affiliation: Department of Histology and Embryology, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Department of Histology and Embryology, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Bin
authors.initials: B
authors.lastname: Shi
authors.affiliation: Department of Histology and Embryology, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Histology and Embryology, Medical College, Nanchang University, Nanchang, Jiangxi 330006, P.R. China.|Jiangxi Province Key Laboratory of Tumor Pathogen's and Molecular Pathology, Nanchang 330006, P.R. China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Chen
mesh_terms: D017667:Adipocytes / Q000378:metabolism; D000818:Animals; D004305:Dose-Response Relationship, Drug; D004958:Estradiol / Q000494:pharmacology* / Q000502:physiology*; D004967:Estrogens / Q000502:physiology; D005260:Female; D020738:Leptin / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D008593:Menopause; D023421:Models, Animal; D010052:Ovariectomy; D010903:Pituitary Gland, Anterior / Q000378:metabolism* / Q000502:physiology*; D012333:RNA, Messenger / Q000378:metabolism; D017207:Rats, Sprague-Dawley; D020239:Receptor Cross-Talk / Q000502:physiology; D054411:Receptors, Leptin / Q000235:genetics / Q000378:metabolism; D050796:STAT3 Transcription Factor / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects* / Q000502:physiology*; D000071223:Suppressor of Cytokine Signaling 3 Protein / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D004967:Estrogens; D020738:Leptin; D012333:RNA, Messenger; D054411:Receptors, Leptin; D050796:STAT3 Transcription Factor; C491410:Socs3 protein, rat; C494088:Stat3 protein, rat; D000071223:Suppressor of Cytokine Signaling 3 Protein; D004958:Estradiol
keywords: 17β-estradiol; anterior pituitary; leptin; leptin receptor; ovariectomized rat