title: Lycopene pretreatment improves hepatotoxicity induced by acetaminophen in C57BL/6 mice.
abstract: Acetaminophen (APAP) is an antipyretic and analgesic drug that, in high doses, leads to severe liver injury and potentially death. Oxidative stress is an important event in APAP overdose. Researchers are looking for natural antioxidants with the potential to mitigate the harmful effects of reactive oxygen species in different models. Lycopene has been widely studied for its antioxidant properties. The aim of this study was to evaluate the antioxidant potential of lycopene pretreatment in APAP-induced liver injury in C57BL/6 mice. C57BL/6 male mice were divided into the following groups: control (C); sunflower oil (CO); acetaminophen 500mg/kg (APAP); acetaminophen 500mg/kg+lycopene 10mg/kg (APAP+L10), and acetaminophen 500mg/kg+lycopene 100mg/kg (APAP+L100). Mice were pretreated with lycopene for 14 consecutive days prior to APAP overdose. Analyses of blood serum and livers were performed. Lycopene was able to improve redox imbalance, decrease thiobarbituric acid reactive species level, and increase CAT and GSH levels. In addition, it decreased the IL-1β expression and the activity of MMP-2. This study revealed that preventive lycopene consumption in C57BL/6 mice can attenuate the effects of APAP-induced liver injury. Furthermore, by improving the redox state, and thus indicating its potential antioxidant effect, lycopene was also shown to have an influence on inflammatory events.
authors.affiliation: Program in Biological Sciences of the Research Center for Biological Sciences - NUPEB, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35.400-000, Brazil; Department of Biological Sciences, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Brazil.
authors.forename: Ana Carla Balthar
authors.initials: ACB
authors.lastname: Bandeira
authors.affiliation: Department of Biological Sciences, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Brazil.
authors.forename: Rafaella Cecília
authors.initials: RC
authors.lastname: da Silva
authors.affiliation: Program in Biological Sciences of the Research Center for Biological Sciences - NUPEB, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35.400-000, Brazil.
authors.forename: Joamyr Victor
authors.initials: JV
authors.lastname: Rossoni
authors.affiliation: Program in Biological Sciences of the Research Center for Biological Sciences - NUPEB, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35.400-000, Brazil.
authors.forename: Vivian Paulino
authors.initials: VP
authors.lastname: Figueiredo
authors.affiliation: Program in Biological Sciences of the Research Center for Biological Sciences - NUPEB, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35.400-000, Brazil.
authors.forename: André
authors.initials: A
authors.lastname: Talvani
authors.affiliation: Department of Biological Sciences, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Brazil.
authors.forename: Silvia Dantas
authors.initials: SD
authors.lastname: Cangussú
authors.affiliation: Program in Biological Sciences of the Research Center for Biological Sciences - NUPEB, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35.400-000, Brazil; Department of Biological Sciences, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Brazil.
authors.forename: Frank Silva
authors.initials: FS
authors.lastname: Bezerra
authors.affiliation: Program in Biological Sciences of the Research Center for Biological Sciences - NUPEB, Federal University of Ouro Preto (UFOP), Ouro Preto, MG 35.400-000, Brazil; Department of Biological Sciences, Universidade Federal de Ouro Preto (UFOP), Ouro Preto, Brazil. Electronic address: dani.caldeiracosta@gmail.com.
authors.forename: Daniela Caldeira
authors.initials: DC
authors.lastname: Costa
mesh_terms: D000082:Acetaminophen / Q000008:administration & dosage; D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D002338:Carotenoids / Q000008:administration & dosage / Q000737:chemistry / Q000494:pharmacology*; D056486:Chemical and Drug Induced Liver Injury / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D004305:Dose-Response Relationship, Drug; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D000077276:Lycopene; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D015394:Molecular Structure; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects; D013329:Structure-Activity Relationship
chemical_list: D000975:Antioxidants; D002338:Carotenoids; D000082:Acetaminophen; D000077276:Lycopene
keywords: Acetaminophen; C57BL/6 mice; Inflammation; Lycopene; Redox imbalance