title: Proteomic Analysis of Differential Expression of Cellular Proteins in Response to Avian H9N2 Virus Infection of A549 Cells.
abstract: In this study, differentially expressed proteins in A549 cells (human lung adenocarcinoma epithelial cell line) infected with H9N2 avian influenza virus (AIV) were investigated by two-dimensional electrophoresis (2-DE). Sixteen different spots between the groups (ratio > 2,p< 0.05) were identified with mass spectrometry identification. Proteins located in the downstream of the NF-κB and IFN transcription factor pathways were identified, e.g., ISG15. Actin and keratin were also identified, suggesting that the cytoskeleton may plays an important role in the AIV infection of mammalian cells. These findings could provide insights into the interaction between host and influenza viruses and might provide valuable information for clarifying the pathogenesis of viral infections as well.
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province Tai'an, China.
authors.forename: Guanliu
authors.initials: G
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province Tai'an, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liang
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province Tai'an, China.
authors.forename: Jiyuan
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province Tai'an, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Meng
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province Tai'an, China.
authors.forename: Liangmeng
authors.initials: L
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province Tai'an, China.
authors.forename: Tongjie
authors.initials: T
authors.lastname: Chai
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Sino-German Cooperative Research Centre for Zoonosis of Animal Origin Shandong Province Tai'an, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Cai
keywords: A549 cells; H9N2 AIV; differentially expressed proteins; proteomics; two-dimensional electrophoresis