title: Grape seed procyanidin B2 inhibits adipogenesis of 3T3-L1 cells by targeting peroxisome proliferator-activated receptor γ with miR-483-5p involved mechanism.
abstract: Procyanidins have lipolysis effect on adipose metabolism, but the underlying mechanism is not fully understood. The aim of present study was to examine the effect of grape seed procyanidin B2 (GSP) on the adipogenic differentiation of 3T3-L1 preadipocyte cell line and investigate the underlying mechanism. The results showed that GSP treatment significantly reduced the intracellular lipid accumulation in induced 3T3-L1 cells by targeting miR-483-5p as well as peroxisome proliferator-activated receptor γ (PPARγ). In addition, our results revealed that overexpression of miR-483-5p increased adipogenic differentiation, while inhibition of miR-483-5p reduced the lipid accumulation by suppressing the adipogenic differentiation. Moreover, overexpression of miR-483-5p could reverse GSP's inhibition of adipocyte differentiation as well as increase the level of PPARγ. These results demonstrate that GSP inhibits adipogenesis by targeting PPARγ and suggest this effect might be mediated by miR-483-5p.
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China; Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
authors.forename: Yazeng
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
authors.forename: Haiyu
authors.initials: H
authors.lastname: Shao
authors.affiliation: Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Bi
authors.affiliation: Department of Orthopedics, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang, China.
authors.forename: Jinping
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, The Second Affiliated Hospital of Zhejiang University School of Medicine, Hangzhou, Zhejiang, China. Electronic address: yezhaoming85@163.com.
authors.forename: Zhaoming
authors.initials: Z
authors.lastname: Ye
mesh_terms: D041721:3T3-L1 Cells; D050156:Adipogenesis / Q000187:drug effects* / Q000502:physiology; D000818:Animals; D044946:Biflavonoids / Q000302:isolation & purification / Q000494:pharmacology*; D002392:Catechin / Q000302:isolation & purification / Q000494:pharmacology*; D018390:Gene Targeting / Q000379:methods; D056604:Grape Seed Extract / Q000302:isolation & purification / Q000494:pharmacology*; D051379:Mice; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics; D047495:PPAR gamma / Q000096:biosynthesis* / Q000235:genetics; D044945:Proanthocyanidins / Q000302:isolation & purification / Q000494:pharmacology*; D027843:Vitis
chemical_list: D044946:Biflavonoids; D056604:Grape Seed Extract; C548912:MIRN483 microRNA, human; D035683:MicroRNAs; D047495:PPAR gamma; D044945:Proanthocyanidins; C017674:procyanidin; D002392:Catechin
keywords: 3T3-L1 adipocytes; Adipogenesis; PPARγ; Procyanidin B2; microRNAs