title: Peripheral and central antinociceptive effects of the butanolic fraction of Byrsonima verbascifolia leaves on nociception-induced models in mice.
abstract: Byrsonima verbascifolia (Malpighiaceae), commonly known as 'murici', is used in folk medicine, for example, in the treatment of inflammation. The anti-inflammatory activity of the butanolic fraction of B. verbascifolia leaves (BvBF) was previously reported by our group, and the present study was designed to evaluate their antinociceptive effects. BvBF (25, 50, and 100 mg/kg) administered intraperitoneally (i.p.) inhibited acetic acid induced abdominal writhing. In the formalin test, BvBF (10, 30 and 100 mg/kg, i.p.) caused a reduction in licking time in both the neurogenic and inflammatory phases. Moreover, we demonstrated that BvBF (30 and 100 mg/kg, i.p.) caused an increase in the latency to response in the hot-plate test. These results demonstrate that BvBF possesses marked peripheral and central antinociceptive activities. Pre-treatment with the non-selective receptor antagonist naloxone (5 mg/kg, i.p.) abolished the antinociceptive effects of BvBF (100 mg/kg, i.p.) in the neurogenic phase of the formalin and hot-plate tests. The anti-inflammatory activity of BvBF (previously reported) as well as the participation of the opioidergic system seems to be responsible, at least in part, for these antinociceptive effects. Finally, BvBF at the doses investigated (25, 50 and 100 mg/Kg) did not cause any toxicity signals, showing that the antinociceptive activity is devoid of sedative and hypomotility effects.
authors.affiliation: Laboratório de Farmacologia, Universidade Federal de São João del-Rei, Divinópolis, 35501-296, Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Saldanha
authors.affiliation: Laboratório de Farmacognosia, Universidade Federal de São João del-Rei, Divinópolis, Brazil, 35501-296.
authors.forename: J M
authors.initials: JM
authors.lastname: Siqueira
authors.affiliation: Laboratório de Farmacobotânica e Plantas Medicinais, Universidade Federal de São João del-Rei, Divinópolis, Brazil, 35501-296.
authors.forename: A H F
authors.initials: AHF
authors.lastname: Castro
authors.affiliation: Departamento de Farmacologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil, 31270-100.
authors.forename: N A
authors.initials: NA
authors.lastname: Matos
authors.affiliation: Departamento de Farmacologia, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil, 31270-100.
authors.forename: A
authors.initials: A
authors.lastname: Klein
authors.affiliation: Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Universidade Federal do Mato Grosso do Sul, Campo Grande, Brazil, 79070-900.
authors.forename: D B
authors.initials: DB
authors.lastname: Silva
authors.affiliation: Laboratório de Produtos Naturais e Espectrometria de Massas (LAPNEM), Universidade Federal do Mato Grosso do Sul, Campo Grande, Brazil, 79070-900.
authors.forename: C A
authors.initials: CA
authors.lastname: Carollo
authors.affiliation: Laboratório de Farmacologia, Universidade Federal de São João del-Rei, Divinópolis, 35501-296, Brazil. adrianasouza@ufsj.edu.br.
authors.forename: A C
authors.initials: AC
authors.lastname: Soares
mesh_terms: D000700:Analgesics / Q000302:isolation & purification / Q000494:pharmacology*; D000818:Animals; D000440:Butanols / Q000494:pharmacology; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D008297:Male; D029581:Malpighiaceae; D051379:Mice; D059225:Nociception / Q000187:drug effects* / Q000502:physiology; D010147:Pain Measurement / Q000187:drug effects* / Q000379:methods; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves
chemical_list: D000700:Analgesics; D000440:Butanols; D010936:Plant Extracts
keywords: Analgesics; Inflammation; Natural products; Nociception; Opioid; Pain