title: Purification of a Human Prostate Specific Antigen.
abstract: Rabbit antiserum raised against the crude extract of normal human prostatic tissue contained antibodies to a prostatic tissue-specific antigen as shown by immunoprecipitation techniques. Using this antiserum a prostate antigen was detected in normal, benign hypertrophic, and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues and showed a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. This report thus presents the first demonstration of the purification of a prostate-specific antigen that does not represent prostatic acid phosphatase.
authors.affiliation: Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York.
authors.forename: M C
authors.initials: MC
authors.lastname: Wang
authors.affiliation: Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York.
authors.forename: L A
authors.initials: LA
authors.lastname: Valenzuela
authors.affiliation: Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York.
authors.forename: G P
authors.initials: GP
authors.lastname: Murphy
authors.affiliation: Department of Diagnostic Immunology Research and Biochemistry, Roswell Park Memorial Institute, Buffalo, New York.
authors.forename: T M
authors.initials: TM
authors.lastname: Chu
mesh_terms: D000818:Animals; D004591:Electrophoresis, Polyacrylamide Gel; D005260:Female; D006801:Humans; D007106:Immune Sera / Q000096:biosynthesis; D047468:Immunoprecipitation; D007525:Isoelectric Focusing; D007610:Kallikreins / Q000032:analysis / Q000302:isolation & purification*; D008297:Male; D011467:Prostate / Q000737:chemistry*; D017430:Prostate-Specific Antigen / Q000032:analysis / Q000302:isolation & purification*; D011471:Prostatic Neoplasms / Q000737:chemistry*; D011817:Rabbits
chemical_list: D007106:Immune Sera; C549870:KLK3 protein, human; D007610:Kallikreins; D017430:Prostate-Specific Antigen