title: Expression Profiling of a Human Thyroid Cell Line Stably Expressing the BRAFV600E Mutation.
abstract: The BRAFV600Emutation acts as an initiator of cancer development in papillary thyroid carcinoma (PTC). Gene expression changes caused by the BRAFV600Emutation may have an important role in thyroid cancer development.
  
  
  
  To study genomic alterations caused by the BRAFV600Emutation, we made human thyroid cell lines that harbor the wild-type BRAF gene (Nthy/WT) and the V600E mutant-type BRAF gene (Nthy/V600E).
  
  
  
  Flow cytometry and western blotting showed stable transfection of the BRAF gene. In functional experiments, Nthy/V600E showed increased anchorage-independent growth and invasion through Matrigel, compared to Nthy/WT. Microarray analysis revealed that 2,441 genes were up-regulated in Nthy/V600E compared to Nthy/WT. Gene ontology analysis showed that the up-regulated genes were associated with cell adhesion, migration, and the ERK and MAPK cascade, and pathway analysis showed enrichment in cancer-related pathways.
  
  
  
  Our Nthy/WT and Nthy/V600E cell line pair could be a suitable model to study the molecular characteristics of BRAFV600EPTC.
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Byoung-Ae
authors.initials: BA
authors.lastname: Kim
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyeon-Gun
authors.initials: HG
authors.lastname: Jee
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.|Department of Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Jin Wook
authors.initials: JW
authors.lastname: Yi
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.|Department of Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Su-Jin
authors.initials: SJ
authors.lastname: Kim
authors.affiliation: Department of Surgery, Seoul National University Boramae Medical Center, Seoul, Republic of Korea.
authors.forename: Young Jun
authors.initials: YJ
authors.lastname: Chai
authors.affiliation: Department of Surgery, Seoul National University Bundang Hospital, Seoul National University College of Medicine, Seongnam, Republic of Korea.
authors.forename: June Young
authors.initials: JY
authors.lastname: Choi
authors.affiliation: Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea kyueunlee@snu.ac.kr.|Department of Surgery, Seoul National University Hospital, Seoul National University College of Medicine, Seoul, Republic of Korea.
authors.forename: Kyu Eun
authors.initials: KE
authors.lastname: Lee
mesh_terms: D002277:Carcinoma / Q000235:genetics / Q000378:metabolism; D002291:Carcinoma, Papillary; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics; D003062:Codon; D005260:Female; D005434:Flow Cytometry; D015870:Gene Expression; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006801:Humans; D008297:Male; D009154:Mutation; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics* / Q000378:metabolism; D017422:Sequence Analysis, DNA; D000077273:Thyroid Cancer, Papillary; D013961:Thyroid Gland / Q000378:metabolism*; D013964:Thyroid Neoplasms / Q000235:genetics / Q000378:metabolism; D014161:Transduction, Genetic
chemical_list: D003062:Codon; D048493:Proto-Oncogene Proteins B-raf
keywords: BRAFV600E; Nthy-ori 3-1 cell; Thyroid cancer; microarrays; signal transduction