title: Expanding the genetic code of Salmonella with non-canonical amino acids.
abstract: The diversity of non-canonical amino acids (ncAAs) endows proteins with new features for a variety of biological studies and biotechnological applications. The genetic code expansion strategy, which co-translationally incorporates ncAAs into specific sites of target proteins, has been applied in many organisms. However, there have been only few studies on pathogens using genetic code expansion. Here, we introduce this technique into the human pathogen Salmonella by incorporating p-azido-phenylalanine, benzoyl-phenylalanine, acetyl-lysine, and phosphoserine into selected Salmonella proteins including a microcompartment shell protein (PduA), a type III secretion effector protein (SteA), and a metabolic enzyme (malate dehydrogenase), and demonstrate practical applications of genetic code expansion in protein labeling, photocrosslinking, and post-translational modification studies in Salmonella. This work will provide powerful tools for a wide range of studies on Salmonella.
authors.affiliation: Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
authors.forename: Qinglei
authors.initials: Q
authors.lastname: Gan
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Brent P
authors.initials: BP
authors.lastname: Lehman
authors.affiliation: Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, IA, 50011, USA.
authors.forename: Thomas A
authors.initials: TA
authors.lastname: Bobik
authors.affiliation: Department of Chemistry and Biochemistry, University of Arkansas, Fayetteville, AR, 72701, USA.
authors.forename: Chenguang
authors.initials: C
authors.lastname: Fan
mesh_terms: D000596:Amino Acids / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D005815:Genetic Code; D012475:Salmonella / Q000235:genetics / Q000378:metabolism
chemical_list: D000596:Amino Acids; D001426:Bacterial Proteins