title: FGF2, FGF3 and FGF4 expression pattern during molars odontogenesis in Didelphis albiventris.
abstract: Odontogenesis is guided by a complex signaling cascade in which several molecules, including FGF2-4, ensure all dental groups development and specificity. Most of the data on odontogenesis derives from rodents, which does not have all dental groups. Didelphis albiventris is an opossum with the closest dentition to humans, and the main odontogenesis stages occur when the newborns are in the pouch. In this study, D. albiventris postnatals were used to characterize the main stages of their molars development; and also to establish FGF2, FGF3 and FGF4 expression pattern. D. albiventris postnatals were processed for histological and indirect immunoperoxidase analysis of the tooth germs. Our results revealed similar dental structures between D. albiventris and mice. However, FGF2, FGF3 and FGF4 expression patterns were observed in a larger number of dental structures, suggesting broader functions for these molecules in this opossum species. The knowledge of the signaling that determinates odontogenesis in an animal model with complete dentition may contribute to the development of therapies for the replacement of lost teeth in humans. This study may also contribute to the implementation of D. albiventris as model for Developmental Biology studies.
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Morfologia, Avenida Presidente Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: iriagds@ufmg.br.
authors.forename: Íria Gabriela Dias
authors.initials: ÍG
authors.lastname: Dos Santos
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Morfologia, Avenida Presidente Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: erika.cris.jorge@gmail.com.
authors.forename: Erika Cristina
authors.initials: EC
authors.lastname: Jorge
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Morfologia, Avenida Presidente Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: aline.copola@gmail.com.
authors.forename: Aline Gonçalves Lio
authors.initials: AG
authors.lastname: Copola
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Morfologia, Avenida Presidente Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: brunobertassoli@gmail.com.
authors.forename: Bruno Machado
authors.initials: BM
authors.lastname: Bertassoli
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Morfologia, Avenida Presidente Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: alfredomgoes@gmail.com.
authors.forename: Alfredo Miranda de
authors.initials: AM
authors.lastname: Goes
authors.affiliation: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas, Departamento de Morfologia, Avenida Presidente Antônio Carlos 6627, CEP 31270-901 Belo Horizonte, Minas Gerais, Brazil. Electronic address: gerluza@icb.ufmg.br.
authors.forename: Gerluza Aparecida Borges
authors.initials: GA
authors.lastname: Silva
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D017124:Conserved Sequence; D048293:Didelphis / Q000254:growth & development / Q000378:metabolism*; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000235:genetics / Q000378:metabolism*; D051518:Fibroblast Growth Factor 3 / Q000235:genetics / Q000378:metabolism*; D051519:Fibroblast Growth Factor 4 / Q000235:genetics / Q000378:metabolism*; D051379:Mice; D008963:Molar / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism; D009805:Odontogenesis
chemical_list: D051518:Fibroblast Growth Factor 3; D051519:Fibroblast Growth Factor 4; D016222:Fibroblast Growth Factor 2
keywords: Didelphis albiventris; FGF2; FGF3; FGF4; Immunohistochemistry; Tooth development