title: High-throughput SRCD using multi-well plates and its applications.
abstract: The sample compartment for high-throughput synchrotron radiation circular dichroism (HT-SRCD) has been developed to satisfy an increased demand of protein characterisation in terms of folding and binding interaction properties not only in the traditional field of structural biology but also in the growing research area of material science with the potential to save time by 80%. As the understanding of protein behaviour in different solvent environments has increased dramatically the development of novel functions such as recombinant proteins modified to have different functions from harvesting solar energy to metabolonics for cleaning heavy and metal and organic molecule pollutions, there is a need to characterise speedily these system.
authors.affiliation: Diamond Light Source, Diamond House, Chilton, Didcot OX11 0DE, United Kingdom.
authors.forename: Rohanah
authors.initials: R
authors.lastname: Hussain
authors.affiliation: Diamond Light Source, Diamond House, Chilton, Didcot OX11 0DE, United Kingdom.
authors.forename: Tamás
authors.initials: T
authors.lastname: Jávorfi
authors.affiliation: Diamond Light Source, Diamond House, Chilton, Didcot OX11 0DE, United Kingdom.
authors.forename: Timothy R
authors.initials: TR
authors.lastname: Rudd
authors.affiliation: Diamond Light Source, Diamond House, Chilton, Didcot OX11 0DE, United Kingdom.
authors.forename: Giuliano
authors.initials: G
authors.lastname: Siligardi
mesh_terms: D002942:Circular Dichroism / Q000295:instrumentation*; D006801:Humans; D017510:Protein Folding; D011506:Proteins / Q000737:chemistry*; D017356:Synchrotrons
chemical_list: D011506:Proteins