title: Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol-Ene or Thiol-Yne Coupling Chemistry.
abstract: Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.
authors.affiliation: Interuniversitary Research Institute for Molecular Recognition and Technological Development (IDM), Chemistry Department, Universitat Politècnica de València , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Maria-Jose
authors.identifier: 0000-0002-2422-7731
authors.initials: MJ
authors.lastname: Bañuls
authors.affiliation: Interuniversitary Research Institute for Molecular Recognition and Technological Development (IDM), Chemistry Department, Universitat Politècnica de València , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Pilar
authors.initials: P
authors.lastname: Jiménez-Meneses
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université de Montpellier , ENSCM, place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
authors.forename: Albert
authors.initials: A
authors.lastname: Meyer
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université de Montpellier , ENSCM, place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
authors.forename: Jean-Jacques
authors.initials: JJ
authors.lastname: Vasseur
authors.affiliation: Institut des Biomolécules Max Mousseron (IBMM), UMR 5247 CNRS, Université de Montpellier , ENSCM, place Eugène Bataillon, CC1704, 34095 Montpellier Cedex 5, France.
authors.forename: François
authors.identifier: 0000-0001-5077-0028
authors.initials: F
authors.lastname: Morvan
authors.affiliation: Interuniversitary Research Institute for Molecular Recognition and Technological Development (IDM), Chemistry Department, Universitat Politècnica de València , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Jorge
authors.identifier: 0000-0001-6756-0991
authors.initials: J
authors.lastname: Escorihuela
authors.affiliation: Interuniversitary Research Institute for Molecular Recognition and Technological Development (IDM), Chemistry Department, Universitat Politècnica de València , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Rosa
authors.initials: R
authors.lastname: Puchades
authors.affiliation: Interuniversitary Research Institute for Molecular Recognition and Technological Development (IDM), Chemistry Department, Universitat Politècnica de València , Camino de Vera s/n, 46022 Valencia, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Maquieira
mesh_terms: D000475:Alkenes / Q000737:chemistry; D000480:Alkynes / Q000737:chemistry; D057930:Click Chemistry; D004269:DNA, Bacterial / Q000032:analysis* / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D015345:Oligonucleotide Probes / Q000737:chemistry* / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods; D012475:Salmonella / Q000737:chemistry / Q000235:genetics*; D013438:Sulfhydryl Compounds / Q000737:chemistry*
chemical_list: D000475:Alkenes; D000480:Alkynes; D004269:DNA, Bacterial; D015345:Oligonucleotide Probes; D013438:Sulfhydryl Compounds