title: Immunoglobulin heavy and light chains and T-cell receptor beta and gamma chains PCR assessment on cytological samples. A study comparing FTA cards and cryopreserved lymph node fine-needle cytology.
abstract: OBJECTIVE
  To evaluate and compare the DNA yield and quality extracted from lymph node fine needle cytology (FNC) samples stored on FTA cards to those cryopreserved, and to assess the immunoglobulin heavy and light chains (IGHK) and T-Cell receptor beta and gamma chains (TCRBG) PCR tests.
  
  
  METHODS
  DNA extractions were performed on FNC of 80 non-Hodgkin lymphomas (NHL), four myelomas and 56 benign reactive hyperplasias (BRH) cryopreserved and stored on FTA cards. The JAK2 gene was amplified to assess the DNA integrity and the IGHK/TCRBG clonality status was tested.
  
  
  RESULTS
  IGHK monoclonality was found in 99% of B-cell NHL and 100% of myeloma. TCRBG monoclonality was found in 100% of T-cell NHL. TCRBG polyclonality was detected in 97% of B-cell NHL, 100% of myeloma and 96% of BRH. IGHK/TCRBG PCR data were confirmed by histological and/or follow-up controls. No differences were found in the DNA quality between cryopreservation and FTA cards storage methods.
  
  
  CONCLUSIONS
  IGHK/TCRBG PCR of the lymphoproliferative process on FTA cards is comparable to those cryopreserved. FTA cards can be used to store lymph node FNC for further molecular investigations.
authors.affiliation: Anatomia Patologica, University of Salerno, Salerno, Italy.|DEA, University "G. Marconi" of Rome, Rome.
authors.forename: A L
authors.initials: AL
authors.lastname: Peluso
authors.affiliation: Department of Public Health, University of Naples 'Federico II', Naples, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Cozzolino
authors.affiliation: Anatomia Patologica, University of Salerno, Salerno, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Bottiglieri
authors.affiliation: Anatomia Patologica, University of Salerno, Salerno, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Lucchese
authors.affiliation: Department of Public Health, University of Naples 'Federico II', Naples, Italy.
authors.forename: R M
authors.initials: RM
authors.lastname: Di Crescenzo
authors.affiliation: UOC Haematology and Transplantation, AOU San Giovanni di Dio e Ruggi D'Aragona, Salerno, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Langella
authors.affiliation: Department of Medicine and Surgery, University of Salerno, Salerno, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Selleri
authors.affiliation: Anatomia Patologica, University of Salerno, Salerno, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Zeppa
mesh_terms: D000328:Adult; D000368:Aged; D044963:Biopsy, Fine-Needle / Q000379:methods; D003581:Cytodiagnosis / Q000379:methods; D005260:Female; D006801:Humans; D007143:Immunoglobulin Heavy Chains / Q000235:genetics*; D007147:Immunoglobulin Light Chains / Q000235:genetics*; D008198:Lymph Nodes / Q000473:pathology*; D008228:Lymphoma, Non-Hodgkin / Q000235:genetics / Q000473:pathology; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction / Q000379:methods; D016693:Receptors, Antigen, T-Cell, alpha-beta / Q000235:genetics*; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics*; D013048:Specimen Handling / Q000379:methods
chemical_list: D007143:Immunoglobulin Heavy Chains; D007147:Immunoglobulin Light Chains; D016693:Receptors, Antigen, T-Cell, alpha-beta; D016692:Receptors, Antigen, T-Cell, gamma-delta
keywords: 
  IGH
  ; 
  IGK
  ; 
  TCRB
  ; 
  TCRG
  ; FTA cards; clonality testing; fine needle cytology; non-Hodgkin lymphoma