title: Hypersensitive antibiotic susceptibility test based on a β-lactamase assay with a parylene-matrix chip.
abstract: Many kinds of susceptibility test for β-lactam antibiotics have been used to determine the antibiotic resistance of bacterial strains. Here, a sensitive antibiotic susceptibility test was presented by using a specialized reaction tool for laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) based on parylene-matrix chip. The β-lactamase assay was carried out in a specialized reaction tool by (1) concentrating the bacterial strain and (2) incubating the bacteria with penicillin-G. The parylene-matrix chip was produced by deposition of a partially porous parylene-N thin film on a dried organic matrix array, and the products of β-lactamase reaction in the low range of mass-to-charge ratio (m/z<500) could be effectively analyzed by using a parylene-matrix chip. The sensing parameters were compared with conventional chromogenic antibiotic susceptibility test for β-lactam antibiotics. Finally, LDI-TOF MS with a specialized reaction tool and parylene-matrix chip could achieve a limit of detection as low as 600 cells/spot for penicillin-G.
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Park
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Jo-Il
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Joo-Yoon
authors.initials: JY
authors.lastname: Noh
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea.
authors.forename: Mira
authors.initials: M
authors.lastname: Kim
authors.affiliation: Korea Institute of Science and Technology (KIST), Seoul, Republic of Korea.
authors.forename: Min-Jung
authors.initials: MJ
authors.lastname: Kang
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50 Yonsei-Ro, Seodaemun-Gu, Seoul, 03722, Republic of Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Pyun
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D001709:Biotechnology; D004926:Escherichia coli / Q000187:drug effects / Q000201:enzymology; D057230:Limit of Detection; D008826:Microbial Sensitivity Tests / Q000295:instrumentation / Q000379:methods* / Q000706:statistics & numerical data; D011108:Polymers; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014992:Xylenes; D065093:beta-Lactamase Inhibitors / Q000494:pharmacology*; D001618:beta-Lactamases / Q000032:analysis*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D011108:Polymers; D014992:Xylenes; D065093:beta-Lactamase Inhibitors; D047090:beta-Lactams; C011055:parylene; D001618:beta-Lactamases
keywords: Antibiotic susceptibility test; Laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS); Parylene-matrix chip; β-lactamase