title: Adipose-derived mesenchymal stem cells ameliorate hyperglycemia through regulating hepatic glucose metabolism in type 2 diabetic rats.
abstract: Infusion of mesenchymal stem cells (MSCs) has been identified in the rapid alleviation in hyperglycemia of diabetic individuals, but the mechanism involved has not been adequately explained by these cells' potential role in modulating system insulin sensitivity and islet regeneration. In this study, we demonstrated adipose-derived mesenchymal stem cells (ASCs) produced significantly lower blood glucose via promoting hepatic glycogen synthesis and inhibiting hepatic glucose production within 24 h after infusion in T2DM rats. In vitro, HepG2 cells treated with palmitate (PA) were used as a model of hepatic glucose metabolism disorder to confirm that ASCs stimulates the phosphorylation of hepatic AMP-activated protein kinase (AMPK) to restores hepatic glucose metabolism in type 2 diabetes. In summary, this study indicated that ASCs improve hyperglycemia via regulating hepatic glucose metabolism. Additionally, the effect of ASCs on hepatic glucose metabolism depended on the AMPK signaling pathway. Thus, this is the new research of the molecular mechanisms of MSCs administration to improve glucose metabolism, and it may indicate a new treatment target of MSCs in T2DM.
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xie
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hao Jie
authors.initials: HJ
authors.lastname: Hao
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Cheng
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Zong Yan
authors.initials: ZY
authors.lastname: Xie
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Ya Qi
authors.initials: YQ
authors.lastname: Yin
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Jie Qing
authors.initials: JQ
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Chinese PLA General Hospital, Beijing 100853, China.
authors.forename: Hong Yu
authors.initials: HY
authors.lastname: Liu
authors.affiliation: Department of Endocrinology, Chinese PLA General Hospital, Beijing 100853, China. Electronic address: muyiming@301hospital.com.cn.
authors.forename: Yi Ming
authors.initials: YM
authors.lastname: Mu
authors.affiliation: Institute of Basic Medicine Science, College of Life Science, Chinese PLA General Hospital, Beijing 100853, China. Electronic address: hanwdrsw69@yahoo.com.
authors.forename: Wei Dong
authors.initials: WD
authors.lastname: Han
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology; D003924:Diabetes Mellitus, Type 2 / Q000378:metabolism* / Q000473:pathology / Q000628:therapy; D004798:Enzymes / Q000378:metabolism; D005947:Glucose / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D056945:Hep G2 Cells / Q000187:drug effects; D006801:Humans; D006943:Hyperglycemia / Q000628:therapy*; D007262:Infusions, Intravenous; D008099:Liver / Q000378:metabolism; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D010168:Palmitates / Q000494:pharmacology; D017207:Rats, Sprague-Dawley
chemical_list: D004798:Enzymes; D010168:Palmitates; D006003:Glycogen; D005947:Glucose
keywords: Hyperglycemia; Insulin resistance; Liver; Mesenchymal stem cells; Type 2 diabetes mellitus