title: iTRAQ-Based Proteomics Identification of Serum Biomarkers of Two Chronic Hepatitis B Subtypes Diagnosed by Traditional Chinese Medicine.
abstract: Background.Chronic infection with hepatitis B virus (HBV) is a leading cause of cirrhosis and hepatocellular carcinoma. By traditional Chinese medicine (TCM) pattern classification, damp heat stasis in the middle-jiao (DHSM) and liver Qi stagnation and spleen deficiency (LSSD) are two most common subtypes of CHB.Results.In this study, we employed iTRAQ proteomics technology to identify potential serum protein biomarkers in 30 LSSD-CHB and 30 DHSM-CHB patients. Of the total 842 detected proteins, 273 and 345 were differentially expressed in LSSD-CHB and DHSM-CHB patients compared to healthy controls, respectively. LSSD-CHB and DHSM-CHB shared 142 upregulated and 84 downregulated proteins, of which several proteins have been reported to be candidate biomarkers, including immunoglobulin (Ig) related proteins, complement components, apolipoproteins, heat shock proteins, insulin-like growth factor binding protein, and alpha-2-macroglobulin. In addition, we identified that proteins might be potential biomarkers to distinguish LSSD-CHB from DHSM-CHB, such as A0A0A0MS51_HUMAN (gelsolin), PON3_HUMAN, Q96K68_HUMAN, and TRPM8_HUMAN that were differentially expressed exclusively in LSSD-CHB patients and A0A087WT59_HUMAN (transthyretin), ITIH1_HUMAN, TSP1_HUMAN, CO5_HUMAN, and ALBU_HUMAN that were differentially expressed specifically in DHSM-CHB patients.Conclusion.This is the first time to report serum proteins in CHB subtype patients. Our findings provide potential biomarkers can be used for LSSD-CHB and DHSM-CHB.
authors.affiliation: Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
authors.forename: Jiankun
authors.identifier: 0000-0003-3536-4077
authors.initials: J
authors.lastname: Yang
authors.affiliation: Handan Chinese Medicine Hospital, Handan 056001, China.
authors.forename: Lichao
authors.identifier: 0000-0001-5309-3288
authors.initials: L
authors.lastname: Yang
authors.affiliation: Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
authors.forename: Baixue
authors.identifier: 0000-0002-1459-8803
authors.initials: B
authors.lastname: Li
authors.affiliation: West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Weilong
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Affiliated Hospital of Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Chengdu Life Baseline Technology, Chengdu 610041, China.
authors.forename: Zhenhua
authors.identifier: 0000-0001-5133-9539
authors.initials: Z
authors.lastname: Zhuang
authors.affiliation: Chengdu Life Baseline Technology, Chengdu 610041, China.
authors.forename: Bin
authors.identifier: 0000-0001-9907-4744
authors.initials: B
authors.lastname: Yang
authors.affiliation: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science (LIMS), La Trobe University, Melbourne, VIC 3086, Australia.
authors.forename: Maoshan
authors.identifier: 0000-0002-3827-4969
authors.initials: M
authors.lastname: Chen
authors.affiliation: Chengdu University of Traditional Chinese Medicine, Chengdu 610075, China.
authors.forename: Quansheng
authors.identifier: 0000-0002-5037-5383
authors.initials: Q
authors.lastname: Feng
mesh_terms: D000293:Adolescent; D000328:Adult; D015415:Biomarkers / Q000097:blood; D001798:Blood Proteins / Q000378:metabolism*; D005260:Female; D019694:Hepatitis B, Chronic / Q000097:blood* / Q000175:diagnosis*; D006801:Humans; D008297:Male; D008516:Medicine, Chinese Traditional; D008875:Middle Aged; D040901:Proteomics
chemical_list: D015415:Biomarkers; D001798:Blood Proteins