title: Quantifying sperm egg interaction to assess the breeding efficiency through artificial insemination in guinea fowls.
abstract: 1. Guinea fowl hens were inseminated weekly once with two doses of spermatozoa (75 million and 100 million) in two different diluents, Beltsville poultry semen extender (BPSE), and Instruments for Veterinary Medicine (IMV), each with and without pre-insemination vaginal douching. Per cent fertility, hatchability, dead germ, dead in shells along with data on sperm egg interaction and vaginal microbial counts were recorded. 2. Artificial insemination had significantly improved the per cent fertility and hatchability compared to natural mating. Dose dependent improvement in fertility was noticed with both diluents. 3. There was a beneficial effect of vaginal douching, which was more pronounced at lower insemination doses. 4. For optimum fertility and hatchability in guinea fowl, insemination of 75 million spermatozoa diluted in BPSE once in 4 d and 100 million spermatozoa diluted in BPSE or IMV once in 5 d coupled with vaginal douching is recommended.
authors.affiliation: a Department of Poultry Science, Madras Veterinary College , Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.
authors.forename: G H
authors.initials: GH
authors.lastname: Hudson
authors.affiliation: b Poultry Research Station , Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.
authors.forename: A V
authors.initials: AV
authors.lastname: Omprakash
authors.affiliation: b Poultry Research Station , Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.
authors.forename: K
authors.initials: K
authors.lastname: Premavalli
authors.affiliation: c TRPVB, Tamil Nadu Veterinary and Animal Sciences University , Chennai , India.
authors.forename: G
authors.initials: G
authors.lastname: Dhinakar Raj
mesh_terms: D000818:Animals; D005260:Female; D005298:Fertility; D046094:Galliformes / Q000502:physiology*; D007315:Insemination, Artificial / Q000592:standards / Q000662:veterinary*; D012662:Semen Preservation / Q000662:veterinary; D013084:Sperm-Ovum Interactions
keywords: Hatchability, dilution, AI; fertility; sperm penetration; vaginal douching