title: Synchronized Fast SPE and UFLC Methods for the Analyses of Eight Antidiabetic Drugs in Human Plasma.
abstract: OBJECTIVE
  The population of diabetic patients is rapidly increasing globally. The treatment of these patients is a complex phenomenon due to the use of the different drugs. The present article reports a synchronized fast SPE-UFLC separation of eight antidiabetic drugs in human plasma. Inexpensive solid phase extraction (SPE) and ultra fast liquid chromatography (UFLC) methods were presented for monitoring of eight antidiabetic drugs in human plasma. The separated drugs include metformin HCl, vildagliptin, gliclazide, linagliptin, sitagliptin, pioglitazone, glimepiride and repaglinide plasma sample.
  
  
  METHODS
  The column used was a Sunshell C18 (150 x 4.6 mm, 2.6 μ) with eluent of acetate buffer (0.05% TEA in 0.05 M NH4Ac of pH 7.0 with H 3PO4) - ACN (60 : 40, v/v). The flow rate was 1.0 mL/min with a detection wavelength of 210 nm and column temp. of 45±1ºC. These drugs were extracted from human plasma using Sep-Pac C18 cartridge. Phosphate buffer (25 mM; pH 7.0) containing these drugs were allowed to pass through cartridge at 0.1 mL/min flow rate. The adsorbed drugs on C18 cartridge were eluted by methanol at 1.0 mL/min flow rate.
  
  
  RESULTS
  The values for the retention, separation and resolution factors were ranged from 0.072 to 9.140, 1.443 to 4.208 and 2.147 to 18.652, correspondingly. The percent recoveries for these drugs in the standard laboratory samples prepared in water ranged from 77 to 88%. These values in plasma samples ranged from 10 to 22%.
  
  
  CONCLUSIONS
  The validated method was fruitfully adopted to analyze these drugs in human plasma for the clinical monitoring of these drugs.
authors.affiliation: Department of Chemistry, Jamia Millia Islamia (Central University), New Delhi-110025, India.
authors.forename: Imran
authors.initials: I
authors.lastname: Ali
authors.affiliation: Quest Pharmaceuticals Pvt. Ltd., Birgunj. Nepal.|School of Basic and Applied Sciences, Galgotias University, Gautam Budh Nagar - 203201, India.
authors.forename: Kamlesh K
authors.initials: KK
authors.lastname: Dutta
authors.affiliation: School of Basic and Applied Sciences, Galgotias University, Gautam Budh Nagar - 203201, India.
authors.forename: Arvind K
authors.initials: AK
authors.lastname: Jain
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451. Saudi Arabia.
authors.forename: Zeid A
authors.initials: ZA
authors.lastname: Alothman
authors.affiliation: Department of Chemistry, College of Science, King Saud University, Riyadh 11451. Saudi Arabia.
authors.forename: Abdulrahman
authors.initials: A
authors.lastname: Alwarthan
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D016903:Drug Monitoring / Q000379:methods; D004867:Equipment Design; D006801:Humans; D007004:Hypoglycemic Agents / Q000097:blood*; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D007004:Hypoglycemic Agents
keywords: SPE; UFLC; antidiabetic drugs; human plasma; sunshell