title: 5 nm Silver Nanoparticles Amplify Clinical Features of Atopic Dermatitis in Mice by Activating Mast Cells.
abstract: The triggering effect of silver nanoparticles (NPs) on the induction of allergic reactions is evaluated, by studying the activation of mast cells and the clinical features of atopic dermatitis in a mouse model. Granule release is induced in RBL-2H3 mast cells by 5 nm, but not 100 nm silver NPs. Increases in the levels of reactive oxygen species (hydrogen peroxide and mitochondrial superoxide) and intracellular Ca++in mast cells are induced by 5 nm silver NPs. In a mouse model of atopic dermatitis induced by a mite allergen, the skin lesions are more severe and appear earlier in mice treated simultaneously with 5 nm silver NPs and allergen compared with mice treated with allergen alone or 100 nm silver NPs and allergen. The histological findings reveal that number of tryptase-positive mast cells and total IgE levels in the serum increase in mice treated with 5 nm silver NPs and allergen. The results in this study indicate that cotreatment with 5 nm silver NPs stimulates mast cell degranulation and induces earlier and more severe clinical alterations in allergy-prone individuals.
authors.affiliation: Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: HanGoo
authors.initials: H
authors.lastname: Kang
authors.affiliation: Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Seungjae
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei University, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Kwang Hoon
authors.initials: KH
authors.lastname: Lee
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei University, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Shan
authors.initials: S
authors.lastname: Jin
authors.affiliation: Department of Dermatology and Cutaneous Biology Research Institute, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei University, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Seo Hyeong
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Kangtaek
authors.initials: K
authors.lastname: Lee
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Hyungjoon
authors.initials: H
authors.lastname: Jeon
authors.affiliation: Department of Chemical and Biomolecular Engineering, Yonsei University, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Young-Geon
authors.initials: YG
authors.lastname: Song
authors.affiliation: Center for Nano-Bio Measurement, Korea Research Institute of Standards and Science, Department of Nano Science, University of Science and Technology, Gajeong-ro 217, Yuseong-gu, Daejeon, 34113, Korea.
authors.forename: Sang-Won
authors.initials: SW
authors.lastname: Lee
authors.affiliation: Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Jinwon
authors.initials: J
authors.lastname: Seo
authors.affiliation: Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: Soomin
authors.initials: S
authors.lastname: Park
authors.affiliation: Department of Microbiology, Institute for Immunology and Immunological Diseases, Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Yonsei-ro 50, Seodaemun-gu, Seoul, 03722, Korea.
authors.forename: In-Hong
authors.initials: IH
authors.lastname: Choi
mesh_terms: D000818:Animals; D016923:Cell Death / Q000187:drug effects; D002460:Cell Line; D003876:Dermatitis, Atopic / Q000097:blood / Q000473:pathology*; D007073:Immunoglobulin E / Q000097:blood; D008407:Mast Cells / Q000187:drug effects / Q000473:pathology*; D053768:Metal Nanoparticles / Q000737:chemistry*; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000187:drug effects / Q000378:metabolism; D010316:Particle Size; D051381:Rats; D017382:Reactive Oxygen Species / Q000378:metabolism; D012834:Silver / Q000737:chemistry* / Q000494:pharmacology; D012867:Skin / Q000187:drug effects / Q000473:pathology; D013481:Superoxides / Q000378:metabolism
chemical_list: D017382:Reactive Oxygen Species; D013481:Superoxides; D007073:Immunoglobulin E; D012834:Silver
keywords: allergic reactions; atopic dermatitis; mast cells; reactive oxygen species; silver nanoparticles