title: The Effects of Destruxin A on Relish and Rel Gene Regulation to the Suspected Immune-Related Genes of Silkworm.
abstract: Destruxin A (DA), a cyclodepsipeptidic mycotoxin of entomopathogenic fungus,Metarhizium anisopliae, has anti-immunity activity against insects, but the mechanism of immune regulation is not clear yet. In our previous experiment, the significant expression changes ofBm_nscaf2838_045,Bm_nscaf2674_066, andBm_nscaf2767_133genes in a silkworm's hemocytes were found, which suggested that these genes might be involved in insect's innate immunity. In the current experiment, the silkworm cell line Bm12 was used to survey the expression levels of these genes after the cells were treated with DA and the transcription factorsBmRel,BmRelish1andBmRelish2were silenced by specific siRNA. The results indicated that, after the cells were treated by DA, the gene expression level ofBmRelish2was significantly downregulated, butBmRelandBmRelish1were not changed. The results also showed that the gene expression levels ofBm_nscaf2838_045andBm_nscaf2674_066had similar phenomena, i.e., downregulation with individualBmRelish1gene silence or DA treatment, upregulation with combination ofBmRelish1gene silence and DA treatment, upregulation with individualBmRelish2gene silence, and downregulation with combination ofBmRelish2gene silence plus DA treatment, but no changes in theBmRelgene silence combined with DA treatment. For theBm_nscaf2767_133gene, the downregulated expressions were found in individualBmRelish2gene silence or DA treatment, upregulation in the combination treatment ofBmRelish2gene silence plus DA, and the individual treatment ofBmRelorBmRelish1silence. It is suggested that expressions of theBm_nscaf2838_045andBm_nscaf2674_066genes are closely related to the Imd signal pathway, butBm_nscaf2767_133genes might involve in both Toll and Imd pathways. Furthermore, theBmRelish1gene acts as an activator and theBmRelish2gene acts as a repressor for bothBm_nscaf2838_045andBm_nscaf2674_066gene expressions. It also implies that DA may participate in the splicing process ofBmRelishwhereBmRelish2was promoted. Our research will provide new insights on the understanding of the activity mechanisms of destruxins.
authors.affiliation: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. hwn688094@163.com.
authors.forename: Weina
authors.initials: W
authors.lastname: Hu
authors.affiliation: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. a1020574494@126.com.
authors.forename: Guangwei
authors.initials: G
authors.lastname: He
authors.affiliation: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. wangjingjing968@126.com.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province, College of Agriculture, South China Agricultural University, Guangzhou 510642, China. hqbscau@scau.edu.cn.
authors.forename: Qiongbo
authors.initials: Q
authors.lastname: Hu
mesh_terms: D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000276:immunology; D002460:Cell Line; D047630:Depsipeptides / Q000494:pharmacology*; D005786:Gene Expression Regulation / Q000187:drug effects*; D007113:Immunity, Innate / Q000187:drug effects* / Q000235:genetics; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D009183:Mycotoxins / Q000494:pharmacology*; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000235:genetics; D015398:Signal Transduction / Q000235:genetics / Q000276:immunology; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation / Q000187:drug effects*
chemical_list: D047630:Depsipeptides; D019476:Insect Proteins; D009183:Mycotoxins; D034741:RNA, Small Interfering; D014157:Transcription Factors; C040423:destruxin A
keywords: Bm12 cell; Imd; Rel; Relish; TollL; destruxin