title: Quantitative Analysis of Contrast-Enhanced Ultrasound Imaging in Invasive Breast Cancer: A Novel Technique to Obtain Histopathologic Information of Microvessel Density.
abstract: We examined whether enhancement area ratios obtained by the new bubble detection method correlate with histologic microvessel density in invasive breast cancer. Forty consecutive patients with invasive breast cancer lesions underwent contrast-enhanced ultrasound. The ratio of enhanced area to manually segmented tumor area (enhancement area ratio) was obtained with the new method at peak and delayed phases (50-54, 55-59, 60-64 and 65-69 s). We also analyzed time-intensity curves to obtain peak intensity and area under curve. Enhancement area ratios in both peak and delayed phases (50-54, 55-59, 60-64 and 65-69 s) were significantly correlated with microvessel density (r = 0.57, 0.62, 0.68, 0.61 and 0.58; p = 0.0001, <0.0001, <.0001, <.0001 and 0.0001, respectively). In time-intensity curve analysis, peak intensity was significantly correlated (r = 0.43, p = 0.0073), whereas area under the curve was not (r = 0.29, p = 0.0769). Enhancement area ratios obtained by the new method were correlated with microvessel density in invasive breast cancer.
authors.affiliation: Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine, Sendai, Japan. Electronic address: naokomori7127@gmail.com.
authors.forename: Naoko
authors.initials: N
authors.lastname: Mori
authors.affiliation: Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Mugikura
authors.affiliation: Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Shoki
authors.initials: S
authors.lastname: Takahashi
authors.affiliation: Department of Information Science, Tohoku University Graduate School, Sendai, Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Ito
authors.affiliation: Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Chiaki
authors.initials: C
authors.lastname: Takasawa
authors.affiliation: Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Li
authors.initials: L
authors.lastname: Li
authors.affiliation: Department of Surgical Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Minoru
authors.initials: M
authors.lastname: Miyashita
authors.affiliation: Department of Pathology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kasajima
authors.affiliation: Department of Orthopedic Surgery, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Yu
authors.initials: Y
authors.lastname: Mori
authors.affiliation: Department of Surgical Oncology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Ishida
authors.affiliation: Department of Biomedical Engineering, Tohoku University Graduate School, Sendai, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kodama
authors.affiliation: Department of Diagnostic Radiology, Tohoku University Graduate School of Medicine, Sendai, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Takase
mesh_terms: D000328:Adult; D000368:Aged; D001940:Breast / Q000098:blood supply / Q000000981:diagnostic imaging / Q000473:pathology; D001943:Breast Neoplasms / Q000098:blood supply / Q000000981:diagnostic imaging* / Q000473:pathology; D003287:Contrast Media; D005069:Evaluation Studies as Topic; D005260:Female; D006801:Humans; D007089:Image Enhancement / Q000379:methods*; D055806:Microvessels; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000000981:diagnostic imaging* / Q000473:pathology; D015203:Reproducibility of Results; D016217:Ultrasonography, Mammary / Q000379:methods*
chemical_list: D003287:Contrast Media
keywords: Breast cancer; Contrast medium; Microvessels; Quantitative evaluation; Ultrasonography