title: The Effects of the Combination of a Refined Carbohydrate Diet and Exposure to Hyperoxia in Mice.
abstract: Obesity is a multifactorial disease with genetic, social, and environmental influences. This study aims at analyzing the effects of the combination of a refined carbohydrate diet and exposure to hyperoxia on the pulmonary oxidative and inflammatory response in mice. Twenty-four mice were divided into four groups: control group (CG), hyperoxia group (HG), refined carbohydrate diet group (RCDG), and refined carbohydrate diet + hyperoxia group (RCDHG). The experimental diet was composed of 10% sugar, 45% standard diet, and 45% sweet condensed milk. For 24 hours, the HG and RCDHG were exposed to hyperoxia and the CG and RCDG to ambient air. After the exposures were completed, the animals were euthanized, and blood, bronchoalveolar lavage fluid, and lungs were collected for analyses. The HG showed higher levels of interferon-γin adipose tissue as compared to other groups and higher levels of interleukin-10 and tumor necrosis factor-αcompared to the CG and RCDHG. SOD and CAT activities in the pulmonary parenchyma decreased in the RCDHG as compared to the CG. There was an increase of lipid peroxidation in the HG, RCDG, and RCDHG as compared to the CG. A refined carbohydrate diet combined with hyperoxia promoted inflammation and redox imbalance in adult mice.
authors.affiliation: Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
authors.forename: Nicia Pedreira
authors.initials: NP
authors.lastname: Soares
authors.affiliation: Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
authors.forename: Keila Karine Duarte
authors.initials: KK
authors.lastname: Campos
authors.affiliation: Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
authors.forename: Karina Braga
authors.initials: KB
authors.lastname: Pena
authors.affiliation: Laboratory of Metabolic Biochemistry (LBM), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
authors.forename: Ana Carla Balthar
authors.initials: AC
authors.lastname: Bandeira
authors.affiliation: Laboratory of Immunobiology of Inflammation (LABIIN), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
authors.forename: André
authors.identifier: 0000-0002-6685-6229
authors.initials: A
authors.lastname: Talvani
authors.affiliation: Laboratory of Experimental Nutrition (LABNEX), School of Nutrition, Ouro Preto, MG, Brazil.
authors.forename: Marcelo Eustáquio
authors.identifier: 0000-0001-5807-6778
authors.initials: ME
authors.lastname: Silva
authors.affiliation: Laboratory of Experimental Pathophysiology (LAFEx), Department of Biological Sciences (DECBI), Center of Research in Biological Sciences (NUPEB), Federal University of Ouro Preto (UFOP), Ouro Preto, MG, Brazil.
authors.forename: Frank Silva
authors.identifier: 0000-0002-0804-5196
authors.initials: FS
authors.lastname: Bezerra
mesh_terms: D017667:Adipocytes / Q000473:pathology; D050154:Adiposity / Q000187:drug effects; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D001786:Blood Glucose / Q000378:metabolism; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology; D002452:Cell Count; D002784:Cholesterol / Q000378:metabolism; D004040:Dietary Carbohydrates / Q000009:adverse effects*; D004822:Epididymis / Q000187:drug effects / Q000473:pathology; D005247:Feeding Behavior; D018496:Hyperoxia / Q000097:blood / Q000473:pathology*; D007118:Immunoassay; D007249:Inflammation / Q000473:pathology; D020738:Leptin / Q000097:blood; D008168:Lung / Q000187:drug effects / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D015430:Weight Gain / Q000187:drug effects
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D001786:Blood Glucose; D004040:Dietary Carbohydrates; D020738:Leptin; D002784:Cholesterol