title: Effect of Fu-Zheng-Xiao-Liu Granules on Expression of Human Epidermal Growth Factor Receptor 2 (HER-2) and Proliferation and Apoptosis of Breast Cancer Cell Line SKBR-3.
abstract: BACKGROUND Previous research showed that granulized Fu-Zheng-Xiao-Liu has a significant effect on breast cancer. However, it remains unclear whether HER-2 plays a role in this anti-cancer effect. MATERIAL AND METHODS Serum of male SD rats administered Fu-Zheng-Xiao-Liu granules (SF) was prepared and used to treat HER-2 positive breast cancer cell line SKBR-3. PBS and herceptin were used as negative and positive controls, respectively. MTT was used to detect the proliferation of SKBR-3 cells. Flow cytometry was used to measure the apoptosis of SKBR-3 cells. Western blot and immunofluorescence were used to measure the expression change of HER-2. RESULTS Serum of male SD rats administered Fu-Zheng-Xiao-Liu granules had significantly reduced HER-2 expression at both mRNA level and protein level, significantly inhibited proliferation of SKBR-3 cells, and significantly increased apoptosis of SKBR-3 cells, compared to that of the blank control group or serum control group. CONCLUSIONS Fu-Zheng-Xiao-Liu granules affect proliferation and apoptosis through inhibition of HER-2 transcription and translation, providing an experimental basis for further study of the mechanism by which Fu-Zheng-Xiao-Liu granules affect breast cancer.
authors.affiliation: Department of Integrated Chinese and Western Medicine, Shenzhen Second People's Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland).
authors.forename: Ting
authors.initials: T
authors.lastname: Mo
authors.affiliation: Department of Integrated Chinese and Western Medicine, Shenzhen Second People's Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland).
authors.forename: Shuangbing
authors.initials: S
authors.lastname: Yue
authors.affiliation: Department of Integrated Chinese and Western Medicine, Shenzhen Second People's Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland).
authors.forename: Huan
authors.initials: H
authors.lastname: Tian
authors.affiliation: Department of Integrated Chinese and Western Medicine, Shenzhen Second People's Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland).
authors.forename: Hong
authors.initials: H
authors.lastname: Lin
authors.affiliation: Department of Integrated Chinese and Western Medicine, Shenzhen Second People's Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland).
authors.forename: Guanglu
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Integrated Chinese and Western Medicine, Shenzhen Second People's Hospital (Shenzhen University First Affiliated Hospital), Shenzhen, Guangdong, China (mainland).
authors.forename: Zili
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D018719:Erb-b2 Receptor Tyrosine Kinases / Q000235:genetics* / Q000378:metabolism; D012307:Risk Factors
chemical_list: D004365:Drugs, Chinese Herbal; D012333:RNA, Messenger; D018719:Erb-b2 Receptor Tyrosine Kinases; C000617852:fu-zheng-xiao-liu; C508053:ERBB2 protein, human