title: Design and characterization of 3D hybrid collagen matrixes as a dermal substitute in skin tissue engineering.
abstract: The highly interconnected porous dressing material was fabricated with the utilization of novel collagen (COL-SPG) for the efficient healing of the wound. Herein, we report the fabrication of 3D collagen impregnated with bioactive extract (COL-SPG-CPE) to get rid of infection at the wound site. The resultant 3D collagen matrix was characterized physiochemically using Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and mechanical property. The dressing substrate possesses the high swelling ability, increase in the porosity, in vitro enzymatic degradability and antibacterial property. The in vitro biocompatibility and fluorescence activity of the collagen scaffold against both NIH 3T3 fibroblast and Human keratinocyte (HaCaT) cell lines assisted in excellent cell adhesion and proliferation over the collagen matrix. Furthermore, the in vivo evaluation of the COL-SPG-CPE 3D sponge exhibited with enhanced collagen synthesis and aids in faster reepithelialization. However, the rate of wound healing was influenced by the expression of vascular endothelial growth factor (VEGF), epidermal growth factor (EGF) and transforming growth factor (TGF-β) growth factors promotes the collagen synthesis, thereby increases the healing efficiency. Based on the results, COL-SPG-CPE has a potential ability in the remodeling of the wound with the 3D collagen as wound dressing material.
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai 600020, Tamilnadu, India.
authors.forename: Giriprasath
authors.initials: G
authors.lastname: Ramanathan
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai 600020, Tamilnadu, India.
authors.forename: Sivakumar
authors.initials: S
authors.lastname: Singaravelu
authors.affiliation: Department of Physiology, College of Veterinary Medicine, Chonbuk National University, 79 Gobong-ro, Iksan-city, Jeollabuk-Do 570-752, Republic of Korea.
authors.forename: Thangavelu
authors.initials: T
authors.lastname: Muthukumar
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai 600020, Tamilnadu, India.
authors.forename: Sitalakshmi
authors.initials: S
authors.lastname: Thyagarajan
authors.affiliation: Organic Chemistry Division, CSIR-Central Leather Research Institute, Adyar, Chennai, 600020, Tamilnadu, India.
authors.forename: Paramasivan Thirumalai
authors.initials: PT
authors.lastname: Perumal
authors.affiliation: Bioproducts Lab, CSIR-Central Leather Research Institute, Chennai 600020, Tamilnadu, India. Electronic address: suma67@gmail.com.
authors.forename: Uma Tiruchirapalli
authors.initials: UT
authors.lastname: Sivagnanam
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D018929:Cell Culture Techniques; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D003094:Collagen / Q000737:chemistry*; D019662:Cucurbitaceae / Q000737:chemistry / Q000378:metabolism; D020405:Dermis / Q000378:metabolism / Q000473:pathology / Q000502:physiology; D004815:Epidermal Growth Factor / Q000378:metabolism; D006801:Humans; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D041681:NIH 3T3 Cells; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D012038:Regeneration; D017768:Skin, Artificial; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D010936:Plant Extracts; D016212:Transforming Growth Factor beta; D042461:Vascular Endothelial Growth Factor A; D004815:Epidermal Growth Factor; D003094:Collagen
keywords: 3D collagen; Arothron stellatus; Dermal substitute; Tissue engineering