title: The role of polymorphism ofinterleukin-2, -10,-13andTNF-α genes in cutaneous T-cell lymphoma pathogenesis.
abstract: BACKGROUND
  As the pathogenesis of cutaneous T-cell lymphomas (CTCL) is not fully understood, inherited gene polymorphisms are considered to play a role in the development of lymphomas.
  
  
  OBJECTIVE
  To investigate whether certain gene polymorphisms might be involved in the development of CTCL.
  
  
  METHODS
  In the case-control study we compared the frequency of nine selected single nucleotide polymorphisms (SNP) of seven genes (rs1800587/-889 C/T of interleukin (IL)-1α, rs2069762/-330G/T)andrs2069763/+166G/T of IL-2, rs1800925/-1112 C/T of IL-13, rs1800896/-1082 A/G of IL-10, rs4073/-251 A/T of IL-8, rs5370/K198N, rs180054/-1370T/G of endothelin-1andrs1800629/-308 G/A of TNF-α) in 43 CTCL and Polish cases using the amplification refractory mutation system polymerase chain reaction method.
  
  
  RESULTS
  We have found that two genotypes, -330GGofIL-2and -1112TTofIL-13both promoter variants associated with "hypertranscription phenotype", were over-represented in CTCL patients compared to healthy controls, and they increase the risk of malignancy development (OR = 5.82,p= 0.001 for IL-2 -330 GG, and OR = 5.67,p= 0.0024 for IL-13 -1112 TT). On the other hand, high transcription -308A allele of theTNF-α gene and -1082GG ofIL-10genotype is less frequent in lymphoma patients and has protective effects on the development of CTCL (OR = 0.45,p= 0.0466 for -308A ofTNF-α, and OR = 0.35,p= 0.0329 for -1082GG ofIL-10genes).
  
  
  CONCLUSIONS
  Our results indicate that hypertranscription promoter variants of IL-2 and IL-13 genes could be estimated as the risk factor for development of CTCL, whileTNF-α andIL-10variants have a protective effect.
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Boguslaw
authors.initials: B
authors.lastname: Nedoszytko
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Berenika
authors.initials: B
authors.lastname: Olszewska
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Jadwiga
authors.initials: J
authors.lastname: Roszkiewicz
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Jolanta
authors.initials: J
authors.lastname: Glen
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Zabłotna
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Hanna
authors.initials: H
authors.lastname: Ługowska-Umer
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Roman
authors.initials: R
authors.lastname: Nowicki
authors.affiliation: Department of Dermatology, Medical University of Gdansk, Gdansk, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Sokołowska-Wojdyło
keywords: cutaneous T-cell lymphoma; cytokine gene polymorphisms