title: Characterization and function analysis of interleukin-1 receptor-associated kinase-1 (IRAK-1) from Fenneropenaeus penicillatus.
abstract: The interleukin-1 receptor-associated kinase-1 (IRAK-1) is an important adapter protein which links downstream of MyD88, and involved in the complex composed of MyD88 and TRAF6 to activate TLRs signaling pathway. In this study, an IRAK-1 homolog (FpIRAK-1) was cloned from the red tail shrimp Fenneropenaeus penicillatus. The ORF of FpIRAK-1 consisted of 2874 bp encoding a protein of 957 amino acids which contains a death domain (DD) and a catalytic domain of serine/threonine kinases (STKc). Homology analysis revealed that the predicted amino acid sequence of FpIRAK-1 shared 71% similarities with IRAK-1 of Litopenaeus vannamei. Real-time RT-PCR indicated that FpIRAK-1 was constitutively expressed in various tissues of F. penicillatus. The expression level of FpIRAK-1 mRNA was significantly up-regulated and then decreased gradually after white spot syndrome virus (WSSV) and Vibrio alginolyticus challenge. Gene knockdown of FpIRAK-1 enhanced the sensitivity of shrimps to WSSV and V. alginolyticus challenge, suggesting FpIRAK-1 could play a positive role against bacterial and viral pathogens. In conclusion, the results of this study provide some insights into the function of FpIRAK-1 in activating Toll signaling pathway and the host defense against invading pathogens.
authors.affiliation: Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University, Qinzhou, China.
authors.forename: Youhou
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang, China.
authors.forename: Yucong
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Fisheries College of Guangdong Ocean University, Zhanjiang, China. Electronic address: shuanghucai@gmail.com.
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Cai
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation; D007113:Immunity, Innate; D053592:Interleukin-1 Receptor-Associated Kinases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D033561:Penaeidae / Q000235:genetics* / Q000276:immunology / Q000382:microbiology / Q000821:virology; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D011897:Random Allocation; D060888:Real-Time Polymerase Chain Reaction; D016415:Sequence Alignment; D015398:Signal Transduction; D044149:Vibrio alginolyticus / Q000502:physiology*; D046848:White spot syndrome virus 1 / Q000502:physiology*
chemical_list: D060829:Arthropod Proteins; D018076:DNA, Complementary; D012333:RNA, Messenger; D053592:Interleukin-1 Receptor-Associated Kinases
keywords: Expression analysis; Fenneropenaeus penicillatus; FpIRAK-1; Gene knockdown; TLRs signaling pathway