title: Association betweenTLR-9polymorphisms and colon cancer susceptibility in Saudi Arabian female patients.
abstract: OBJECTIVE
  The authors aimed to explore the relationship between the expression/polymorphisms ofTLR-9and susceptibility to colon cancer development in the Saudi Arabian population.
  
  
  METHODS
  In total, blood samples from 115 patients with colon cancer and 102 participants without colon cancer were analyzed in this study. Three single-nucleotide polymorphisms (SNPs) were selected from theTLR-9gene, including two sites within theTLR-9gene's promoter region (rs352144 and rs187084) and one site in aTLR-9intron region (rs5743839). Odds ratios (ORs) and 95% confidence intervals (CIs) were computed from logistic regression models after adjusting for age, gender, and tumor localization. To investigate the differential expression ofTLR-9in colon cancer,TLR-9expression was evaluated using quantitative real-time reverse transcription polymerase chain reaction on 40 matched normal and colon tissues.
  
  
  RESULTS
  The authors found thatTLR-9expression was decreased in colon cancer tissues as compared with that in normal tissues. Moreover, significant associations between theTLR-9rs187084 SNP and colon cancer risk were observed in female patients only. In rs187084, the T allele had a significantly lower frequency (2.8 times) in female cancer patients than in controls (0.27 vs 0.41). TheTLR-9rs352139 and rs352144 SNPs were significantly associated with colon cancer development when the tumor was located in the rectal area.
  
  
  CONCLUSIONS
  The findings support the hypothesis thatTLR-9has an anticancer role in colon cancer development. Furthermore, genetic variation may influence colon cancer development, and SNPs inTLR-9could serve as biomarkers for decision making in the treatment of females with rectal cancer.
authors.affiliation: Genome Research, Department of Biochemistry, College of Sciences, King Saud University.
authors.forename: Abdelhabib
authors.initials: A
authors.lastname: Semlali
authors.affiliation: Genome Research, Department of Biochemistry, College of Sciences, King Saud University.
authors.forename: Narasimha Reddy
authors.initials: NR
authors.lastname: Parine
authors.affiliation: Genome Research, Department of Biochemistry, College of Sciences, King Saud University.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Al Amri
authors.affiliation: College of Medicine, Al Imam Muhammad Ibn Saud Islamic University.
authors.forename: Arezki
authors.initials: A
authors.lastname: Azzi
authors.affiliation: College of Medicine, King Saud University.
authors.forename: Maha
authors.initials: M
authors.lastname: Arafah
authors.affiliation: Genome Research, Department of Biochemistry, College of Sciences, King Saud University.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Kohailan
authors.affiliation: Genome Research, Department of Biochemistry, College of Sciences, King Saud University.
authors.forename: Jilani P
authors.initials: JP
authors.lastname: Shaik
authors.affiliation: Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Kingdom of Saudi Arabia; Division of Gastroenterology, McGill University Health Center, Montreal General Hospital, Montreal.
authors.forename: Majid Abdulrahman
authors.initials: MA
authors.lastname: Almadi
authors.affiliation: College of Medicine, King Saud University; Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdulrahman M
authors.initials: AM
authors.lastname: Aljebreen
authors.affiliation: College of Medicine, King Saud University; Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Othman
authors.initials: O
authors.lastname: Alharbi
authors.affiliation: College of Medicine, King Saud University; Division of Gastroenterology, King Khalid University Hospital, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Nahla
authors.initials: N
authors.lastname: Ali Azzam
authors.affiliation: Groupe de Recherche en Écologie Buccale, Département de Stomatologie, Faculté de Médecine Dentaire, Université Laval, Québec City, QC, Canada.
authors.forename: Mahmoud
authors.initials: M
authors.lastname: Rouabhia
authors.affiliation: Genome Research, Department of Biochemistry, College of Sciences, King Saud University.
authors.forename: Mohammad Saud
authors.initials: MS
authors.lastname: Alanazi
keywords: Innate; TLR polymorphisms; immunity; rs187084; rs352139; rs352144