title: Lipopolysaccharide-induced α-catenin downregulation enhances the motility of human colorectal cancer cells in an NF-κB signaling-dependent manner.
abstract: α-Catenin is an important molecule involved in the maintenance of cell-cell adhesion and a prognostic marker in cancer since its expression is essential for preventing cancer metastasis. However, the mechanism that leads to the downregulation of α-catenin in cancer progression remains unclear. The present study revealed that lipopolysaccharide (LPS)-induced NF-κB signaling activation suppressed α-catenin expression and motility in SW620 colorectal cancer (CRC) cells, using real-time polymerase chain reaction, Western blotting, and transwell migration assays. LPS treatment reduced both the mRNA and protein expression of α-catenin and thereby enhanced cell motility. Conversely, incubating cells with an NF-κB inhibitor disrupted these effects. Furthermore, the ectopic expression of p65 alone mimicked the effects of LPS stimulation. In CRC tissues, the presence of enteric bacterial LPS-related neutrophil-enriched foci was correlated with α-catenin downregulation. Collectively, these findings suggest that LPS-induced NF-κB signaling is related to α-catenin suppression and enhanced cell motility in CRC. Therefore, NF-κB is a novel potential therapeutic target for CRC metastasis.
authors.affiliation: Department of Pathology, Zhejiang Cancer Hospital; Cancer Research Institute, Zhejiang Cancer Hospital and Key laboratory Diagnosis and Treatment Technology on Thoracic Oncology of Zhejiang Province.
authors.forename: Guoping
authors.initials: G
authors.lastname: Cheng
authors.affiliation: Department of Pathology, Zhejiang Cancer Hospital.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Pathology, Zhejiang Cancer Hospital.
authors.forename: Gu
authors.initials: G
authors.lastname: Zhang
authors.affiliation: School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Xu
authors.affiliation: School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Zhejiang Cancer Hospital; Cancer Research Institute, Zhejiang Cancer Hospital and Key laboratory Diagnosis and Treatment Technology on Thoracic Oncology of Zhejiang Province.
authors.forename: Wenyong
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Medicine, Hangzhou Normal University, Hangzhou, Zhejiang, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhu
keywords: colorectal neoplasms; lipopolysaccharide; neoplasm metastasis; α-catenin