title: Optimized procedures for manganese-52: Production, separation and radiolabeling.
abstract: Pressed chromium-powder cyclotron targets were irradiated with 16MeV protons, producing52Mn with average yields of 6.2±0.8MBq/µAh. Separation by solid-phase anion exchange from ethanol-HCl mixtures recovered 94.3±1.7% of52Mn and reduced the chromium content by a factor of 2.2±0.4×105. An additional AG 1-X8 column was used to remove copper, iron, cobalt and zinc impurities from the prepared52Mn in 8M HCl. The macrocyclic chelator DOTA was rapidly radiolabeled with52Mn in aq. ammonium acetate (pH 7.5R.T.) with a radiochemical yield >99% within 1min and was stable for >2 days in bovine serum. The improved separation and purification methodology facilitates the use of52Mn in basic science and preclinical investigations.
authors.affiliation: Hevesy Laboratory, Center for Nuclear Technologies, Technical University of Denmark, Frederiksborgvej 399, 4000 Roskilde, Denmark.
authors.forename: Jesper
authors.initials: J
authors.lastname: Fonslet
authors.affiliation: Hevesy Laboratory, Center for Nuclear Technologies, Technical University of Denmark, Frederiksborgvej 399, 4000 Roskilde, Denmark.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Tietze
authors.affiliation: Hevesy Laboratory, Center for Nuclear Technologies, Technical University of Denmark, Frederiksborgvej 399, 4000 Roskilde, Denmark.
authors.forename: Andreas I
authors.initials: AI
authors.lastname: Jensen
authors.affiliation: Department of Medical Physics, University of Wisconsin - Madison, Madison, WI 53706, United States.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Graves
authors.affiliation: Hevesy Laboratory, Center for Nuclear Technologies, Technical University of Denmark, Frederiksborgvej 399, 4000 Roskilde, Denmark; Department of Chemistry, Michigan State University, East Lansing, MI 48824, United States; Facility for Rare Isotope Beams, Michigan State University, East Lansing, MI 48824, United States. Electronic address: gwseverin@chemistry.msu.edu.
authors.forename: Gregory W
authors.initials: GW
authors.lastname: Severin
keywords: (52)Mn; Cyclotron; DOTA; Isotope production; Manganese; Mn-52; PET; Serum stability