title: Isolation and characterization of 2-pyridone alkaloids and alloxazines from Beauveria bassiana.
abstract: Two novel compounds bearing heterocyclic nitrogen, 2-pyridone alkaloid (1) and alloxazine derivative (2), along with the known pretenellin B (3), pyridovericin (4) and lumichrome (5) were isolated from a culture of the entomopathogenic fungal strain Beauveria bassiana. The chemical structures of 2-pyridone alkaloid and alloxazine derivative were established on the basis of the interpretation of spectroscopic data. The isolated compounds were evaluated in a panel of five cancer cell lines and pyridovericin exhibited cytotoxicity (IC50, μM) against cancer cell lines: HL-60 (25.9 ± 0.3), HCT8 (34.6 ± 3.6), MDA-MB435 (34.8 ± 3.8) and SF295 (31.1 ± 0.6). Considering that other pyridone compounds display good cytotoxic activity, it would be suggested to obtain new semi synthetic derivatives of pyridovericin, for the development of new cytotoxic chemical entities.
authors.affiliation: a Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto , Brazil.|d Laboratório de Produtos Bioativos , Universidade Federal do Rio de Janeiro , Macaé , Brazil.
authors.forename: W J
authors.initials: WJ
authors.lastname: Andrioli
authors.affiliation: a Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto , Brazil.|e Unidade de Biotecnologia , Universidade de Ribeirão Preto , Ribeirão Preto , Brazil.
authors.forename: A A
authors.initials: AA
authors.lastname: Lopes
authors.affiliation: b Departamento de Fisiologia e de Farmacologia , Universidade Federal do Ceará , Fortaleza , Brazil.
authors.forename: B C
authors.initials: BC
authors.lastname: Cavalcanti
authors.affiliation: b Departamento de Fisiologia e de Farmacologia , Universidade Federal do Ceará , Fortaleza , Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Pessoa
authors.affiliation: c National Center for Natural Products Research , University of Mississippi , Oxford , MS , USA.
authors.forename: N P D
authors.initials: NPD
authors.lastname: Nanayakkara
authors.affiliation: a Faculdade de Ciências Farmacêuticas de Ribeirão Preto , Universidade de São Paulo , Ribeirão Preto , Brazil.
authors.forename: J K
authors.initials: JK
authors.lastname: Bastos
mesh_terms: D000470:Alkaloids / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology; D000970:Antineoplastic Agents / Q000737:chemistry / Q000494:pharmacology*; D052982:Beauveria / Q000737:chemistry* / Q000378:metabolism; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005415:Flavins / Q000737:chemistry / Q000302:isolation & purification; D006801:Humans; D015394:Molecular Structure; D009005:Monosaccharides / Q000737:chemistry; D011728:Pyridones / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D064210:Secondary Metabolism
chemical_list: D000470:Alkaloids; D000970:Antineoplastic Agents; D005415:Flavins; D009005:Monosaccharides; D011728:Pyridones; C114116:pyridovericin; C001559:7,8-dimethylalloxazine; C008173:isoalloxazine; C017679:2-hydroxypyridine
keywords: Alloxazines; Beauveria bassiana; entomopathogenic fungus; pyridone alkaloids