title: Enhanced yield of ethylene glycol production from d-xylose by pathway optimization in Escherichia coli.
abstract: The microbial production of renewable ethylene glycol (EG) has been gaining attention recently due to its growing importance in chemical and polymer industries. EG has been successfully produced biosynthetically from d-xylose through several novel pathways. The first report on EG biosynthesis employed the Dahms pathway in Escherichia coli wherein 71% of the theoretical yield was achieved. This report further improved the EG yield by implementing metabolic engineering strategies. First, d-xylonic acid accumulation was reduced by employing a weak promoter which provided a tighter control over Xdh expression. Second, EG yield was further improved by expressing the YjgB, which was identified as the most suitable aldehyde reductase endogenous to E. coli. Finally, cellular growth, d-xylose consumption, and EG yield were further increased by blocking a competing reaction. The final strain (WTXB) was able to reach up to 98% of the theoretical yield (25% higher as compared to the first study), the highest reported value for EG production from d-xylose.
authors.affiliation: Department of Energy Science and Technology (DEST), Energy and Environment Fusion Technology Center (E(2)FTC), Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, 170-58, South Korea.
authors.forename: Rhudith B
authors.initials: RB
authors.lastname: Cabulong
authors.affiliation: Department of Energy Science and Technology (DEST), Energy and Environment Fusion Technology Center (E(2)FTC), Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, 170-58, South Korea.
authors.forename: Kris Niño G
authors.initials: KN
authors.lastname: Valdehuesa
authors.affiliation: Department of Energy Science and Technology (DEST), Energy and Environment Fusion Technology Center (E(2)FTC), Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, 170-58, South Korea.
authors.forename: Kristine Rose M
authors.initials: KR
authors.lastname: Ramos
authors.affiliation: Department of Energy Science and Technology (DEST), Energy and Environment Fusion Technology Center (E(2)FTC), Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, 170-58, South Korea.
authors.forename: Grace M
authors.initials: GM
authors.lastname: Nisola
authors.affiliation: Division of Bioscience and Bioinformatics, Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, 170-58, South Korea.
authors.forename: Won-Keun
authors.initials: WK
authors.lastname: Lee
authors.affiliation: Division of Bioscience and Bioinformatics, Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, 170-58, South Korea.
authors.forename: Chang Ro
authors.initials: CR
authors.lastname: Lee
authors.affiliation: Department of Energy Science and Technology (DEST), Energy and Environment Fusion Technology Center (E(2)FTC), Myongji University, Myongji-ro 116, Cheoin-gu, Yongin, Gyeonggi-do, 170-58, South Korea. Electronic address: wjc0828@gmail.com.
authors.forename: Wook-Jin
authors.initials: WJ
authors.lastname: Chung
mesh_terms: D000429:Alcohol Oxidoreductases / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism*; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D019855:Ethylene Glycol / Q000378:metabolism*; D005285:Fermentation; D007218:Industrial Microbiology; D060847:Metabolic Engineering / Q000379:methods; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014994:Xylose / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D011994:Recombinant Proteins; D014994:Xylose; D000429:Alcohol Oxidoreductases; C585286:YjgB protein, E coli; C035795:D-xylose dehydrogenase; D019855:Ethylene Glycol
keywords: Aldehyde reductase; Dahms pathway; Ethylene glycol; Metabolic engineering; YjgB; d-xylose