title: Methyl jasmonate induces the apoptosis of human colorectal cancer cells via downregulation of EZH2 expression by microRNA‑101.
abstract: Methyl jasmonate is found universally in the plant kingdom and functions to regulate plant growth and development, as well as in stress responses through signal transduction pathways. The present study aimed to investigate the anticancer effect of methyl jasmonate on SW620 human colorectal cancer cells and its potential underlying mechanism. SW620 cells were treated with 0, 0.5, 0.75, 1.5 and 2.0 mM methyl jasmonate for 12, 24 and 48 h. Methyl jasmonate was shown to be able to inhibit cell growth and induce apoptosis of SW620 cells in a concentration and time‑dependent manner, whilst promoting an increase in caspase‑3 protein expression. Compared with control, the anticancer effect of methyl jasmonate inhibited Enhancer of zeste homolog 2 (EZH2) protein expression and activated microRNA (miR)‑101 expression in SW620 cells. However, knockdown of miR‑101 suppresses methyl jasmonate‑induced cell growth inhibition, activation of caspase‑3 expression and inhibition of EZH2 expression in SW620 cells. These results demonstrate that methyl jasmonate induced the apoptosis of human colorectal cancer cells via downregulation of EZH2 expression by miR‑101.
authors.affiliation: Department of General Surgery, PLA General Hospital, PLA Postgraduate School of Medicine, Beijing 100853, P.R. China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of General Surgery, PLA General Hospital, PLA Postgraduate School of Medicine, Beijing 100853, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000085:Acetates / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D045744:Cell Line, Tumor; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D015179:Colorectal Neoplasms / Q000188:drug therapy* / Q000235:genetics / Q000473:pathology; D003517:Cyclopentanes / Q000494:pharmacology*; D015536:Down-Regulation / Q000187:drug effects; D000071221:Enhancer of Zeste Homolog 2 Protein / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D054883:Oxylipins / Q000494:pharmacology*; D010937:Plant Growth Regulators / Q000494:pharmacology; D012007:Rectum / Q000187:drug effects / Q000378:metabolism / Q000473:pathology
chemical_list: D000085:Acetates; D000972:Antineoplastic Agents, Phytogenic; D003517:Cyclopentanes; C534072:MIRN101 microRNA, human; D035683:MicroRNAs; D054883:Oxylipins; D010937:Plant Growth Regulators; C072239:methyl jasmonate; D000071221:Enhancer of Zeste Homolog 2 Protein