title: Simultaneous Determination and Pharmacokinetic Study of Six Components in Rat Plasma by HPLC-MS/MS after Oral Administration of Acanthopanax sessiliflorus Fruit Extract.
abstract: A specific and reliable HPLC-MS/MS method was developed and validated for the simultaneous determination of protocatechuic acid (PCA), scopolin, (-)-pinoresinol-4,4'-di-O-β-d-glucopyranoside (PDG), acanthoside D, acanthoside B and hyperin in rat plasma for the first time. The analytes were separated on a C18column (50 × 2.1 mm, 1.8 µm) and a triple-quadrupole mass spectrometer equipped with an electrospray ionization (ESI) source was used for detection. The rat plasma sample was prepared using the protein precipitation procedure. The calibration curves were linear over a concentration range of 1.2-1200.0 ng/mL for PCA, 0.96-960.0 ng/mL for scopolin, 1.12-1120.0 ng/mL for PDG, 1.32-1320.0 ng/mL for acanthoside D, 0.99-990.0 ng/mL for acanthoside B and 1.01-1010.0 ng/mL for hyperin. The intra-day and inter-day precision was less than 11.4% and the relative error (RE) was all within ±15%. The validated method was successfully applied to assess the pharmacokinetics characteristics after the extracts ofAcanthopanax sessiliflorusfruits were orally administered to the Sprague-Dawley rat.
authors.affiliation: College of Pharmacy, Soochow University, Suzhou 215123, China. Dupeng_gyyxy@hotmail.com.|Drug Clinical Trial Institution, the Affiliated Hospital of Guizhou Medical University, Guiyang 550004, China. Dupeng_gyyxy@hotmail.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.affiliation: Department of Pharmacy, Jiangxi Maternal and Child Health Hospital, No. 318 Bayi Road, Nanchang 330001, China. leimingdaojxnc@163.com.
authors.forename: Mingdao
authors.initials: M
authors.lastname: Lei
authors.affiliation: College of Pharmacy, Liaoning University, Shenyang 110036, China. liuyu1981@163.com.
authors.forename: Yu
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Pharmacy, Soochow University, Suzhou 215123, China. sdyangsl@sina.com.
authors.forename: Shilin
authors.initials: S
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid; D003374:Coumarins / Q000032:analysis / Q000097:blood; D028001:Eleutherococcus / Q000737:chemistry*; D005663:Furans / Q000032:analysis / Q000097:blood; D005960:Glucosides / Q000032:analysis / Q000097:blood; D017705:Lignans / Q000032:analysis / Q000097:blood; D008297:Male; D013058:Mass Spectrometry; D010936:Plant Extracts / Q000008:administration & dosage / Q000097:blood / Q000737:chemistry*; D011794:Quercetin / Q000031:analogs & derivatives / Q000032:analysis / Q000097:blood; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D003374:Coumarins; D005663:Furans; D005960:Glucosides; D017705:Lignans; D010936:Plant Extracts; C528660:acanthoside D; C417572:scopolin; C021304:hyperoside; D011794:Quercetin
keywords: Acanthopanax sessiliflorus fruits; HPLC-MS/MS; pharmacokinetics; rat plasma