title: Visualization of silver-decorated poly (DL-lactide-co-glycolide) nanoparticles and their efficacy against Staphylococcus epidermidis.
abstract: Understanding of self-protection activity of the bacteria and interaction with drug substances has significant importance for designing of effective drug delivery system for treatment of biofilm infections. Recently silver nanoparticle has attracted attention as antibacterial substance for drug delivery system because of its high antibacterial activity. Here, efflux of silver nanoparticles obtained from within the prepared silver-decorated poly (DL-lactide-co-glycolide) (Ag PLGA) nanoparticles derived from Staphylococcus epidermidis bacterial cell was successfully visualized using scanning transmission electron microscopy (STEM). We also revealed the interaction between prepared Ag PLGA nanoparticles and the bacterial cells at the nanoscale level using field emission scanning electron microscopy and STEM, after a pretreatment process by an ionic liquid. This finding is significant to understand a fundamental function of S. epidermidis bacterial cells, which is not explored previously. The results suggest that Ag PLGA nanoparticles could demonstrate high efficacy against biofilm infections.
authors.affiliation: Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan. Electronic address: chisato@dpc.agu.ac.jp.
authors.forename: Chisato
authors.initials: C
authors.lastname: Takahashi
authors.affiliation: Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Matsubara
authors.affiliation: Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Akachi
authors.affiliation: Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Ogawa
authors.affiliation: Department of Frontier Materials, Nagoya Institute of Technology, Gokisocho, Showa-ku, Nagoya 466-8555, Japan.
authors.forename: Golap
authors.initials: G
authors.lastname: Kalita
authors.affiliation: Department of Materials Science and Engineering, Nagoya Institute of Technology, Gokisocho, Showa-ku, Nagoya 466-8555, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Asaka
authors.affiliation: Department of Frontier Materials, Nagoya Institute of Technology, Gokisocho, Showa-ku, Nagoya 466-8555, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Tanemura
authors.affiliation: Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
authors.forename: Yoshiaki
authors.initials: Y
authors.lastname: Kawashima
authors.affiliation: Pharmaceutical Engineering, School of Pharmacy, Aichi Gakuin University, 1-100, Kusumoto-cho, Chikusa-ku, Nagoya 464-8650, Japan.
authors.forename: Hiromitsu
authors.initials: H
authors.lastname: Yamamoto
mesh_terms: D018441:Biofilms / Q000187:drug effects; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000633:toxicity; D008855:Microscopy, Electron, Scanning; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D012834:Silver / Q000737:chemistry*; D013212:Staphylococcus epidermidis / Q000502:physiology*
chemical_list: D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D012834:Silver
keywords: Biofilm; Drug delivery system; Electron microscopy; Polymeric nanoparticle; Self-protection; Silver