title: Customized Array Comparative Genomic Hybridization Analysis of 25 Phosphatase-encoding Genes in Colorectal Cancer Tissues.
abstract: Molecular mechanisms of alterations in protein tyrosine phosphatases (PTPs) genes in cancer have been previously described and include chromosomal aberrations, gene mutations, and epigenetic silencing. However, little is known about small intragenic gains and losses that may lead to either changes in expression or enzyme activity and even loss of protein function.
  
  
  
  The aim of this study was to investigate 25 phosphatase genes using customized array comparative genomic hybridization in 16 sporadic colorectal cancer tissues.
  
  
  
  The analysis revealed two unique small alterations: of 2 kb in PTPN14 intron 1 and of 1 kb in PTPRJ intron 1. We also found gains and losses of whole PTPs gene sequences covered by large chromosome aberrations.
  
  
  
  In our preliminary studies using high-resolution custom microarray we confirmed that PTPs are frequently subjected to whole-gene rearrangements in colorectal cancer, and we revealed that non-polymorphic intragenic changes are rare.
authors.affiliation: Genetics Department, Wroclaw Medical University, Wroclaw, Poland iza.laczmanska@gmail.com.
authors.forename: Izabela
authors.initials: I
authors.lastname: Laczmanska
authors.affiliation: Genetics Department, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Skiba
authors.affiliation: Genetics Department, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Karpinski
authors.affiliation: 1st Department of Surgical Oncology, Lower Silesian Oncology Center, Wroclaw, Poland.
authors.forename: Marek
authors.initials: M
authors.lastname: Bebenek
authors.affiliation: Genetics Department, Wroclaw Medical University, Wroclaw, Poland.
authors.forename: Maria M
authors.initials: MM
authors.lastname: Sasiadek
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D002869:Chromosome Aberrations; D002874:Chromosome Mapping; D015179:Colorectal Neoplasms / Q000235:genetics* / Q000378:metabolism; D055028:Comparative Genomic Hybridization; D044127:Epigenesis, Genetic; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D005810:Multigene Family; D010744:Phosphoric Monoester Hydrolases / Q000235:genetics* / Q000378:metabolism; D017027:Protein Tyrosine Phosphatases / Q000235:genetics; D048493:Proto-Oncogene Proteins B-raf / Q000235:genetics / Q000378:metabolism; D016283:Proto-Oncogene Proteins p21(ras) / Q000235:genetics
chemical_list: C117307:KRAS protein, human; D048493:Proto-Oncogene Proteins B-raf; D010744:Phosphoric Monoester Hydrolases; D017027:Protein Tyrosine Phosphatases; D016283:Proto-Oncogene Proteins p21(ras)
keywords: aCGH; colorectal cancer; protein tyrosine phosphatases