title: Dengue Virus Serotypes Circulating in Khyber Pakhtunkhwa Province, Pakistan, 2013-2015.
abstract: From 2013 to 2015, the National Institute of Health, Pakistan, received 1,270 blood samples of suspected dengue cases reported from inpatient and outpatient departments of various hospitals in Khyber Pakhtunkhwa (KPK) province. In this study, we determined the circulating dengue virus (DENV) serotypes using real-time reverse transcriptase (RT)-PCR to understand the serotype-based epidemiology of DENV. All four serotypes (DENV-1 [6%], DENV-2 [33%], DENV-3 [47%], and DENV-4 [0.1%]) were found circulating during the study period. Our findings suggest the need for an active surveillance system coupled with the laboratory diagnosis, especially in the chronic endemic areas of the country. Public awareness programs are needed for effective control and prevention of outbreaks in the future.
authors.affiliation: Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.|Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Suleman
authors.affiliation: Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.
authors.forename: Rani
authors.initials: R
authors.lastname: Faryal
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Muhammad Masroor
authors.initials: MM
authors.lastname: Alam
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Salmaan
authors.initials: S
authors.lastname: Sharif
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Shahzad
authors.initials: S
authors.lastname: Shaukat
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Uzma Bashir
authors.initials: UB
authors.lastname: Aamir
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Adnan
authors.initials: A
authors.lastname: Khurshid
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Mehar
authors.initials: M
authors.lastname: Angez
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Massab
authors.initials: M
authors.lastname: Umair
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Mian Muhammad
authors.initials: MM
authors.lastname: Sufian
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan.
authors.forename: Yasir
authors.initials: Y
authors.lastname: Arshad
authors.affiliation: Department of Virology, National Institute of Health, Park Road, Chak Shahzad, Islamabad, Pakistan. sohailz@live.com.
authors.forename: Syed Sohail Zahoor
authors.initials: SS
authors.lastname: Zaidi
mesh_terms: D000293:Adolescent; D000328:Adult; D003715:Dengue / Q000175:diagnosis / Q000453:epidemiology* / Q000821:virology; D003716:Dengue Virus / Q000235:genetics / Q000302:isolation & purification*; D004196:Disease Outbreaks; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010154:Pakistan / Q000453:epidemiology; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D065288:Serogroup; D055815:Young Adult
chemical_list: D012367:RNA, Viral
keywords: Dengue; Epidemiology; Pakistan; Real-time RT-PCR; Serotypes