title: Expression of Bruton's tyrosine kinase in B-cell neoplasms evaluated by flow cytometry.
abstract: Bruton's tyrosine kinase (BTK) is a cytoplasmatic protein that is part of the B-cell antigen receptor signaling pathway. Our aim was to evaluate the expression of BTK in B-cell neoplasms and compare it to normal B-cell lymphocytes. After surface staining with CD19 and CD45, flow cytometry staining for intracellular BTK was performed in leukemic or mature B-cells from bone marrow or peripheral blood samples. No differences in BTK expression were identified between groups, or in comparison to control samples, there was no association between BTK expression and the clinical variables evaluated. BTK expression in B-cell neoplasms was similar to that of normal B-cell lymphocytes.
authors.affiliation: Programa de Pós Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2400, 2° andar, Porto Alegre, 90035-903, Brazil. nam_nati@yahoo.com.br.|Laboratório Zanol, Rua Mostardeiro 333 sala 120, Porto Alegre, 90430-001, Brazil. nam_nati@yahoo.com.br.
authors.forename: Natália Aydos
authors.initials: NA
authors.lastname: Marcondes
authors.affiliation: Laboratório Zanol, Rua Mostardeiro 333 sala 120, Porto Alegre, 90430-001, Brazil.
authors.forename: Flavo Beno
authors.initials: FB
authors.lastname: Fernandes
authors.affiliation: Unidade de Diagnóstico Personalizado, Serviço de Patologia Clínica, Hospital de Clínicas de Porto Alegre, Rua Ramiro Barcelos 2350, Porto Alegre, 90035-903, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Alegretti
authors.affiliation: Programa de Pós Graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos 2400, 2° andar, Porto Alegre, 90035-903, Brazil.|Laboratório Zanol, Rua Mostardeiro 333 sala 120, Porto Alegre, 90430-001, Brazil.|Departamento de Medicina Interna, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcelos, 2350, Porto Alegre, 90035-903, Brazil.
authors.forename: Gustavo Adolpho Moreira
authors.initials: GAM
authors.lastname: Faulhaber
mesh_terms: D000293:Adolescent; D000328:Adult; D000077329:Agammaglobulinaemia Tyrosine Kinase; D000368:Aged; D000369:Aged, 80 and over; D005260:Female; D005434:Flow Cytometry; D006801:Humans; D016393:Lymphoma, B-Cell / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D011505:Protein-Tyrosine Kinases / Q000032:analysis*; D055815:Young Adult
chemical_list: D011505:Protein-Tyrosine Kinases; D000077329:Agammaglobulinaemia Tyrosine Kinase; C000625949:BTK protein, human
keywords: B-cell neoplasm; BTK; Bruton’s tyrosine kinase; Flow cytometry