title: Microcontact imprinted quartz crystal microbalance nanosensor for protein C recognition.
abstract: Detection of protein C (PC) in human serum was performed by quartz crystal microbalance (QCM) based on molecular imprinting technique (MIP). The high-resolution and mass-sensitive QCM based sensor was integrated with high sensitivity and selectivity of the MIP technique. The PC microcontact imprinted (PC-μCIP) nanofilm was prepared on the glass surface. Then, the PC-μCIP/QCM sensor was prepared with 2-hydroxyethyl methacrylate (HEMA), ethylene glycol dimethacrylate (EGDMA) and N-methacryloyl l-histidine methylester (MAH) as the functional monomer with copper(II) ions. The polymerization was performed under UV light (100W and 365nm) for 20-25min under nitrogen atmosphere. The characterization studies of QCM sensor were done by observation using atomic force microscopy (AFM), contact angle measurements, ellipsometry and fourier transform infrared spectroscopy (FTIR). Detection of PC was investigated in a concentration range of 0.1-30μg/mL. Selectivity of PC-μCIP and PC non-imprinted/QCM (PC-non-μCIP) sensors for PC determination was investigated by using proteins namely hemoglobin (Hb), human serum albumin (HSA) and fibrinogen solutions. QCM sensor was also used for detection of PC molecules in aqueous solutions and human plasma. The detection limit was determined as 0.01μg/mL for PC analysis. The PC-μCIP/QCM sensor was used for five consecutive adsorption-desorption cycles. According to the results, the PC-μCIP/QCM sensor had obtained high selectivity and sensitivity for detection of PC molecules.
authors.affiliation: Hacettepe University, Department of Chemistry, Beytepe, Ankara, Turkey.
authors.forename: Monireh
authors.initials: M
authors.lastname: Bakhshpour
authors.affiliation: Hacettepe University, Department of Chemistry, Beytepe, Ankara, Turkey.
authors.forename: Erdoğan
authors.initials: E
authors.lastname: Özgür
authors.affiliation: Hacettepe University, Department of Chemistry, Beytepe, Ankara, Turkey.
authors.forename: Nilay
authors.initials: N
authors.lastname: Bereli
authors.affiliation: Hacettepe University, Department of Chemistry, Beytepe, Ankara, Turkey. Electronic address: denizli@hacettepe.edu.tr.
authors.forename: Adil
authors.initials: A
authors.lastname: Denizli
mesh_terms: D005340:Fibrinogen / Q000032:analysis; D005898:Glass; D006454:Hemoglobins / Q000032:analysis; D006801:Humans; D007700:Kinetics; D057230:Limit of Detection; D008689:Methacrylates / Q000737:chemistry; D018625:Microscopy, Atomic Force; D008962:Models, Theoretical; D054802:Molecular Imprinting; D036103:Nanotechnology / Q000295:instrumentation*; D009584:Nitrogen / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D011486:Protein C / Q000032:analysis*; D058525:Quartz Crystal Microbalance Techniques; D015203:Reproducibility of Results; D012709:Serum Albumin / Q000032:analysis; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D013696:Temperature
chemical_list: D006454:Hemoglobins; D008689:Methacrylates; D011108:Polymers; D011486:Protein C; D012709:Serum Albumin; C004919:ethylene dimethacrylate; D005340:Fibrinogen; D009584:Nitrogen
keywords: Microcontact imprinting; Protein C; Protein detection; Quartz crystal microbalance