title: Attraction of Coffee Bean Weevil, Araecerus fasciculatus, to Volatiles from the Industrial Yeast Kluyveromyces lactis.
abstract: The coffee bean weevil (CBW), Araecerus fasciculatus (De Geer, 1775) (Coleoptera: Anthribidae) is an important pest of stored products such as grains, coffee beans, cassava, and traditional Chinese medicine materials. In China, CBW causes large losses of Daqu, a traditional Chinese liquor fermentation starter, and, unfortunately, the use of conventional insecticides against CBW is not suitable in Daqu storage. We found CBW to be highly attracted to fermenting yeast cultures, such as Kluyveromyces lactis. Eight volatile compounds, produced by fermenting cultures and not by sterile samples, were identified by gas chromatography coupled with mass spectrometry. Five of these substances elicited significant responses in Y-tube behavioral bioassays. Field trapping experiments revealed 2-phenylethanol and 2-phenylethyl acetate to be crucial for attraction of CBW. Results show that yeast volatiles play an important role in host location, and that 2-phenylethanol and 2-phenylethyl acetate could be utilized as potential attractants in monitoring and control systems against this important pest.
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No 2 West Yuan Ming Yuan Road, Beijing, 100193, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Yang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No 2 West Yuan Ming Yuan Road, Beijing, 100193, China.
authors.forename: Xiang-Dong
authors.initials: XD
authors.lastname: Mei
authors.affiliation: Institute of Plant Protection, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Hebei Academy of Agricultural and Forestry Sciences, 437 Dongguan Street, Baoding City, Hebei Province, 071000, China.
authors.forename: Xiao-Fang
authors.initials: XF
authors.lastname: Zhang
authors.affiliation: Institute of Plant Protection, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Hebei Academy of Agricultural and Forestry Sciences, 437 Dongguan Street, Baoding City, Hebei Province, 071000, China.
authors.forename: Yao-Fa
authors.initials: YF
authors.lastname: Li
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No 2 West Yuan Ming Yuan Road, Beijing, 100193, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: She
authors.affiliation: Institute of Plant Protection, Integrated Pest Management Center of Hebei Province, Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture, Hebei Academy of Agricultural and Forestry Sciences, 437 Dongguan Street, Baoding City, Hebei Province, 071000, China. cauzht@163.com.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No 2 West Yuan Ming Yuan Road, Beijing, 100193, China. jning@ippcaas.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Ning
mesh_terms: D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects*; D003069:Coffee / Q000469:parasitology*; D008401:Gas Chromatography-Mass Spectrometry; D007300:Insect Control / Q000379:methods*; D007716:Kluyveromyces / Q000378:metabolism*; D012639:Seeds / Q000469:parasitology; D055549:Volatile Organic Compounds / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D048889:Weevils / Q000502:physiology*
chemical_list: D003069:Coffee; D055549:Volatile Organic Compounds
keywords: Araecerus fasciculatus; Behavioral bioassay; Coffee bean weevil; Kluyveromyces lactis; Trapping experiment; Volatile; Yeast