title: DNA damage and repair capacity in lymphocyte of chronic obstructive pulmonary diseases patients during physical exercise with oxygen supplementation.
abstract: BACKGROUND
  We hypothesized that the use of oxygen supplementation during aerobic exercise induces less DNA damage than exercise alone. The aim of this study is to assess the level of DNA damage induced by physical exercise with and without oxygen supplementation in chronic obstructive pulmonary diseases (COPD) patients.
  
  
  METHODS
  Peripheral blood was collected before and after aerobic exercise in two conditions: (I) aerobic exercise without oxygen supplementation (AE group) and (II) with oxygen supplementation (AE-O2group). Lymphocytes were collected to perform the alkaline version of the Comet Assay. To assess the susceptibility to exogenous DNA damage, the lymphocytes were treated with methyl methanesulphonate (MMS) for 1-h or 3-h. After 3-h treatment, the percentage of residual damage was calculated assuming the value of 1-h MMS treatment as 100%.
  
  
  RESULTS
  AE group showed lower induced damage (1 h of MMS treatment) and consequently less DNA repair compared to AE-O2group. AE-O2group showed an increase in the induced DNA damage (1 h of MMS treatment) and an increased DNA repair capacity. Within the AE-O2group, in the post-exercise situation the induced DNA damage after 1 h of MMS treatment was higher (p= 0.01) than in the pre-exercise.
  
  
  CONCLUSIONS
  COPD patients who performed physical exercise associated with oxygen supplementation had a better response to DNA damage induced by MMS and a better DNA repair when compared to the condition of physical exercise without oxygen supplementation.
  
  
  BACKGROUND
  UNISC N374.298. Registered 04 JUN 2013 (retrospectively registered).
authors.affiliation: Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul - UNISC, Avenida Independência, 2293, Bairro Universitário, Santa Cruz do Sul, RS CEP 96815-900 Brazil ; Hospital Santa Cruz, Santa Cruz do Sul, RS Brazil.
authors.forename: Andréa Lúcia Gonçalves
authors.identifier: 0000-0001-8731-689X
authors.initials: AL
authors.lastname: da Silva
authors.affiliation: Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS Brazil.
authors.forename: Thaís Evelyn
authors.initials: TE
authors.lastname: Karnopp
authors.affiliation: Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS Brazil.
authors.forename: Augusto Ferreira
authors.initials: AF
authors.lastname: Weber
authors.affiliation: Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul - UNISC, Avenida Independência, 2293, Bairro Universitário, Santa Cruz do Sul, RS CEP 96815-900 Brazil.
authors.forename: Cassia da Luz
authors.initials: CD
authors.lastname: Goulart
authors.affiliation: Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul - UNISC, Avenida Independência, 2293, Bairro Universitário, Santa Cruz do Sul, RS CEP 96815-900 Brazil.
authors.forename: Paloma de Borba
authors.initials: PB
authors.lastname: Scheneiders
authors.affiliation: Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul - UNISC, Avenida Independência, 2293, Bairro Universitário, Santa Cruz do Sul, RS CEP 96815-900 Brazil.
authors.forename: Dannuey Machado
authors.initials: DM
authors.lastname: Cardoso
authors.affiliation: Departamento de Educação Física e Saúde, Universidade de Santa Cruz do Sul - UNISC, Avenida Independência, 2293, Bairro Universitário, Santa Cruz do Sul, RS CEP 96815-900 Brazil ; Hospital Santa Cruz, Santa Cruz do Sul, RS Brazil.
authors.forename: Lisiane Lisboa
authors.initials: LL
authors.lastname: Carvalho
authors.affiliation: Programa de Pós-Graduação em Genética e Biologia Molecular, Departamento de Genética, Universidade Federal do Rio Grande do Sul - UFRGS, Porto Alegre, RS Brazil.
authors.forename: Joel Henrique
authors.initials: JH
authors.lastname: Ellwanger
authors.affiliation: Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS Brazil ; Programa de Pós-Graduação em Promoção da Saúde, Universidade de Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS Brazil.
authors.forename: Lia Gonçalves
authors.initials: LG
authors.lastname: Possuelo
authors.affiliation: Departamento de Biologia e Farmácia, Universidade de Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS Brazil ; Programa de Pós-Graduação em Promoção da Saúde, Universidade de Santa Cruz do Sul - UNISC, Santa Cruz do Sul, RS Brazil.
authors.forename: Andréia Rosane de Moura
authors.initials: AR
authors.lastname: Valim
keywords: Aerobic exercise; COPD; Comet assay; DNA repair; Induced DNA damage