title: Characterization of transfer function, resolution and depth of field of a soft X-ray microscope applied to tomography enhancement by Wiener deconvolution.
abstract: Full field soft X-ray microscopy is becoming a powerful imaging technique to analyze whole cells preserved under cryo conditions. Images obtained in these X-ray microscopes can be combined by tomographic reconstruction to quantitatively estimate the three-dimensional (3D) distribution of absorption coefficients inside the cell. The impulse response of an imaging system is one of the factors that limits the quality of the X-ray microscope reconstructions. The main goal of this work is to experimentally measure the 3D impulse response and to assess the optical resolution and depth of field of the Mistral microscope at ALBA synchrotron (Barcelona, Spain). To this end we measure the microscope apparent transfer function (ATF) and we use it to design a deblurring Wiener filter, obtaining an increase in the image quality when applied to experimental datasets collected at ALBA.
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049, Madrid, Spain.
authors.forename: Joaquín
authors.initials: J
authors.lastname: Otón
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, 08290, Barcelona, Spain.
authors.forename: Eva
authors.initials: E
authors.lastname: Pereiro
authors.affiliation: ALBA Synchrotron Light Source, Cerdanyola del Vallès, 08290, Barcelona, Spain.
authors.forename: Ana J
authors.initials: AJ
authors.lastname: Pérez-Berná
authors.affiliation: Facultat de Biociències. Departament de Genètica i Microbiologia. UAB. Cerdanyola del Vallès, 08193, Barcelona, Spain.
authors.forename: Laia
authors.initials: L
authors.lastname: Millach
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049, Madrid, Spain.
authors.forename: Carlos Oscar S
authors.initials: CO
authors.lastname: Sorzano
authors.affiliation: Escuela Politecnica Superior, Univ. Autonoma de Madrid, Cantoblanco, 28049, Madrid, Spain.
authors.forename: Roberto
authors.initials: R
authors.lastname: Marabini
authors.affiliation: Centro Nacional de Biotecnología (CNB-CSIC), Cantoblanco, 28049, Madrid, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Carazo
keywords: (040.7480) X-rays, soft x-rays, extreme ultraviolet (EUV); (100.1830) Deconvolution; (110.4100) Modulation transfer function; (110.6960) Tomography; (110.7440) X-ray imaging; (180.7460) X-ray microscopy; (340.0340) X-ray optics; (350.5730) Resolution