title: A Programmable DNA Double-Write Material: Synergy of Photolithography and Self-Assembly Nanofabrication.
abstract: We demonstrate a DNA double-write process that uses UV to pattern a uniquely designed DNA write material, which produces two distinct binding identities for hybridizing two different complementary DNA sequences. The process requires no modification to the DNA by chemical reagents and allows programmed DNA self-assembly and further UV patterning in the UV exposed and nonexposed areas. Multilayered DNA patterning with hybridization of fluorescently labeled complementary DNA sequences, biotin probe/fluorescent streptavidin complexes, and DNA patterns with 500 nm line widths were all demonstrated.
authors.affiliation: Department of Electrical and Computer Engineering, ‡Department of Mechanical Engineering, §Department of Materials Science and Engineering, ∥Department of Nanoengineering, and ⊥Department of Bioengineering, University of California San Diego , La Jolla, California 92093-0448, United States.
authors.forename: Youngjun
authors.initials: Y
authors.lastname: Song
authors.affiliation: Department of Electrical and Computer Engineering, ‡Department of Mechanical Engineering, §Department of Materials Science and Engineering, ∥Department of Nanoengineering, and ⊥Department of Bioengineering, University of California San Diego , La Jolla, California 92093-0448, United States.
authors.forename: Tsukasa
authors.initials: T
authors.lastname: Takahashi
authors.affiliation: Department of Electrical and Computer Engineering, ‡Department of Mechanical Engineering, §Department of Materials Science and Engineering, ∥Department of Nanoengineering, and ⊥Department of Bioengineering, University of California San Diego , La Jolla, California 92093-0448, United States.
authors.forename: Sejung
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Electrical and Computer Engineering, ‡Department of Mechanical Engineering, §Department of Materials Science and Engineering, ∥Department of Nanoengineering, and ⊥Department of Bioengineering, University of California San Diego , La Jolla, California 92093-0448, United States.
authors.forename: Yvonne C
authors.initials: YC
authors.lastname: Heaney
authors.affiliation: Department of Electrical and Computer Engineering, ‡Department of Mechanical Engineering, §Department of Materials Science and Engineering, ∥Department of Nanoengineering, and ⊥Department of Bioengineering, University of California San Diego , La Jolla, California 92093-0448, United States.
authors.forename: John
authors.initials: J
authors.lastname: Warner
authors.affiliation: Department of Electrical and Computer Engineering, ‡Department of Mechanical Engineering, §Department of Materials Science and Engineering, ∥Department of Nanoengineering, and ⊥Department of Bioengineering, University of California San Diego , La Jolla, California 92093-0448, United States.
authors.forename: Shaochen
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Electrical and Computer Engineering, ‡Department of Mechanical Engineering, §Department of Materials Science and Engineering, ∥Department of Nanoengineering, and ⊥Department of Bioengineering, University of California San Diego , La Jolla, California 92093-0448, United States.
authors.forename: Michael J
authors.identifier: 0000-0001-6436-0337
authors.initials: MJ
authors.lastname: Heller
mesh_terms: D004247:DNA; D018076:DNA, Complementary; D007202:Indicators and Reagents; D057139:Nanofibers / Q000737:chemistry*; D009693:Nucleic Acid Hybridization; D019809:Streptavidin
chemical_list: D018076:DNA, Complementary; D007202:Indicators and Reagents; D004247:DNA; D019809:Streptavidin
keywords: DNA; displacer effect; patterning; photolithography; self-assembly; thymine dimer