title: Gut content microbiota of introduced bigheaded carps (Hypophthalmichthys spp.) inhabiting the largest shallow lake in Central Europe.
abstract: Studying the microbiota in the alimentary tract of bigheaded carps (Hypophthalmichthys spp.) gained special interest recently, as these types of investigations on non-native fish species may lead to a better understanding of their ecological role and feeding habits in an invaded habitat. For microbiological examinations, bigheaded carp gut contents and water column samples from Lake Balaton (Hungary) were collected from spring to autumn in 2013. Denaturing Gradient Gel Electrophoresis (DGGE) and pyrosequencing of the 16S rRNA gene were performed to reveal the composition. According to the DGGE patterns, bacterial communities of water samples separated clearly from that of the intestines. Moreover, the bacterial communities in the foreguts and hindguts were also strikingly dissimilar. Based on pyrosequencing, both foregut and hindgut samples were predominated by the fermentative genus Cetobacterium (Fusobacteria). The presence of some phytoplankton taxa and the high relative abundance of cellulose-degrading bacteria in the guts suggest that intestinal microbes may have an important role in digesting algae and making them utilizable for bigheaded carps that lack cellulase enzyme. In turn, the complete absence of typical heterotrophic freshwater bacteria in all studied sections of the intestines indicated that bacterioplankton probably has a negligible role in the nutrition of bigheaded carps.
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary. Electronic address: borsodi.andrea@ttk.elte.hu.
authors.forename: Andrea K
authors.initials: AK
authors.lastname: Borsodi
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: Attila
authors.initials: A
authors.lastname: Szabó
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Krett
authors.affiliation: Department of Microbiology, Eötvös Loránd University, Pázmány P. sétány 1/C, H-1117 Budapest, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Felföldi
authors.affiliation: Balaton Limnological Institute, MTA Centre for Ecological Research, Klebelsberg K. u. 3, H-8237 Tihany, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Specziár
authors.affiliation: Balaton Limnological Institute, MTA Centre for Ecological Research, Klebelsberg K. u. 3, H-8237 Tihany, Hungary.
authors.forename: Gergely
authors.initials: G
authors.lastname: Boros
mesh_terms: D000818:Animals; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D002347:Carps / Q000382:microbiology*; D016000:Cluster Analysis; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D058645:Denaturing Gradient Gel Electrophoresis; D056890:Eukaryota / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D000069196:Gastrointestinal Microbiome; D041981:Gastrointestinal Tract / Q000382:microbiology; D006814:Hungary; D060106:Lakes; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004275:DNA, Ribosomal; D012336:RNA, Ribosomal, 16S
keywords: Bigheaded carp; Fusobacteria; Hypophthalmichthys spp.; Intestinal microbiota; Lake Balaton