title: Gastroprotective effects of hydroethanolic root extract of Arrabidaea brachypoda: Evidences of cytoprotection and isolation of unusual glycosylated polyphenols.
abstract: The hydroethanolic root extract of Arrabidaea brachypoda, from Bignoniaceae family, a Brazilian medicinal plant, demonstrated significant in vivo gastroprotective effects using different in vivo assays. The activity was evaluated in several models of experimental gastric ulcer in rats (absolute ethanol, glutathione depletion, nitric oxide depletion, non-steroidal anti-inflammatory drugs, pylorus ligation and acetic acid). Using 300 mg/kg (p.o.) the extract significantly reduced gastric injury in all models. In depth phytochemical investigation of this extract led to the isolation of two previously undescribed phenylethanoid glycosides derivatives and seven unusual glycosylated dimeric flavonoids. The structures were elucidated using UV, NMR and HRMS analysis. Absolute configuration of the dimeric flavonoids was performed by electronic circular dichroism (ECD) spectroscopy.
authors.affiliation: Institute of Biosciences, Coastal Campus of São Vicente, Universidade Estadual Paulista-UNESP, 11330-900, São Vicente, SP, Brazil; School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Centre Medical Universitaire-CMU, Rue Michel-Servet 1, CH-1211, Geneva 4, Switzerland.
authors.forename: Claudia Quintino
authors.initials: CQ
authors.lastname: da Rocha
authors.affiliation: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil.
authors.forename: Felipe Meira
authors.initials: FM
authors.lastname: de-Faria
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Centre Medical Universitaire-CMU, Rue Michel-Servet 1, CH-1211, Geneva 4, Switzerland.
authors.forename: Laurence
authors.initials: L
authors.lastname: Marcourt
authors.affiliation: Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, G. C., Evin, Tehran, Iran.
authors.forename: Samad Nejad
authors.initials: SN
authors.lastname: Ebrahimi
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil.
authors.forename: Bruna Tiemi
authors.initials: BT
authors.lastname: Kitano
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil.
authors.forename: Amanda Franceschini
authors.initials: AF
authors.lastname: Ghilardi
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil; Nucleus of Biological Sciences, Institute of Biotechnology, Federal University of Goias, 75704-020, Catalão, GO, Brazil.
authors.forename: Anderson
authors.initials: A
authors.lastname: Luiz Ferreira
authors.affiliation: Department of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil.
authors.forename: Ana Cristina Alves
authors.initials: AC
authors.lastname: de Almeida
authors.affiliation: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil.
authors.forename: Ricardo José
authors.initials: RJ
authors.lastname: Dunder
authors.affiliation: Department of Pharmacology, Faculty of Medical Sciences, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil; Department of Structural and Functional Biology, Institute of Biology, State University of Campinas-UNICAMP, 13083-970, Campinas, SP, Brazil.
authors.forename: Alba Regina Monteiro
authors.initials: AR
authors.lastname: Souza-Brito
authors.affiliation: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Klingelbergstrasse 50, CH-4056 Basel, Switzerland.
authors.forename: Matthias
authors.initials: M
authors.lastname: Hamburger
authors.affiliation: Institute of Biosciences, Coastal Campus of São Vicente, Universidade Estadual Paulista-UNESP, 11330-900, São Vicente, SP, Brazil. Electronic address: vilegasw@gmail.com.
authors.forename: Wagner
authors.initials: W
authors.lastname: Vilegas
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Centre Medical Universitaire-CMU, Rue Michel-Servet 1, CH-1211, Geneva 4, Switzerland. Electronic address: emerson.ferreira@unige.ch.
authors.forename: Emerson Ferreira
authors.initials: EF
authors.lastname: Queiroz
authors.affiliation: School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Centre Medical Universitaire-CMU, Rue Michel-Servet 1, CH-1211, Geneva 4, Switzerland.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Wolfender
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D000897:Anti-Ulcer Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D029402:Bignoniaceae / Q000737:chemistry*; D001938:Brazil; D019610:Cytoprotection; D005419:Flavonoids / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D008297:Male; D018515:Plant Leaves / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry*; D059808:Polyphenols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D013276:Stomach Ulcer / Q000188:drug therapy
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000897:Anti-Ulcer Agents; D005419:Flavonoids; D006027:Glycosides; D059808:Polyphenols
keywords: Arrabidaea brachypoda; Bignoniaceae; Gastric ulcer; Gastroprotective effect; Glycosylated dimeric flavonoids; In vivo assays