title: Myeloperoxidase in blood neutrophils during normal and abnormal menstrual cycles in women of reproductive age.
abstract: BACKGROUND
  We previously reported that granulocyte colony-stimulating factor (G-CSF) plays a critical role in ovulation, suggesting that neutrophils may maintain ovulation. We assessed myeloperoxidase (MPO), a major and specific enzyme of neutrophils, in women with abnormal and normal menstrual cycles to clarify the relationship between MPO and ovulation.
  
  
  METHODS
  We analyzed MPO activity in blood neutrophils of women with abnormal menstrual cycles (indicative of anovulation, n = 12) and age- and body mass index-matched normal menstrual cycles (indicative of ovulation, n = 24) using two parameters as a marker of MPO, Neut X and mean peroxidase index (MPXI).
  
  
  RESULTS
  MPO of women with abnormal menstrual cycles was significantly lower than that of women with normal menstrual cycles [Neut X: 62.6 ± 1.1 (mean ± standard error of the mean) vs. 66.2 ± 0.3, P = 0.009; MPXI: -0.54 ± 1.66 vs. 4.91 ± 0.53, P = 0.008]. Among women with normal menstrual cycles, MPO was highest in the follicular phase (Neut X: 67.0 ± 0.3; P = 0.033).
  
  
  CONCLUSIONS
  The difference in MPO between women with abnormal and normal menstrual cycles and the upregulation of MPO before ovulation suggest that neutrophils and MPO are closely related to ovulation.
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: J
authors.initials: J
authors.lastname: Sakamoto
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Osaka
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: N
authors.initials: N
authors.lastname: Neyatani
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Fujita
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Oka
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Takagi
authors.affiliation: Department of Central Clinical Laboratory, Kanazawa Medical University Hospital, Uchinada, Ishikawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Mori
authors.affiliation: Department of Central Clinical Laboratory, Kanazawa Medical University Hospital, Uchinada, Ishikawa, Japan.
authors.forename: H
authors.initials: H
authors.lastname: Fujita
authors.affiliation: Department of Central Clinical Laboratory, Kanazawa Medical University Hospital, Uchinada, Ishikawa, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Tanaka
authors.affiliation: Department of Obstetrics and Gynecology, Kanazawa Medical University, Uchinada, Ishikawa, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Sasagawa
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005498:Follicular Phase; D006801:Humans; D008597:Menstrual Cycle / Q000097:blood*; D008599:Menstruation Disturbances / Q000097:blood / Q000201:enzymology; D009504:Neutrophils / Q000201:enzymology*; D010060:Ovulation; D009195:Peroxidase / Q000032:analysis*
chemical_list: D009195:Peroxidase
keywords: Anovulation; menstrual cycle; myeloperoxidase; neutrophil; ovulation