title: Effect of fulvic acid induction on the physiology, metabolism, and lipid biosynthesis-related gene transcription of Monoraphidium sp. FXY-10.
abstract: Fulvic acid (FA) triggers lipid accumulation in Monoraphidium sp. FXY-10, which can produce biofuels. Therefore, the metabolism shift and gene expression changes influenced by fulvic acid should be investigated. In this study, lipid and protein contents increased rapidly from 44.6% to 54.3% and from 31.4% to 39.7% under FA treatment, respectively. By contrast, carbohydrate content sharply declined from 49.5% to 32.5%. The correlation between lipid content and gene expression was also analyzed. Results revealed that accD, ME, and GPAT genes were significantly correlated with lipid accumulation. These genes could likely influence lipid accumulation and could be selected as modification candidates. These results demonstrated that FA significantly increased microalgal lipid accumulation by changing the intracellular reactive oxygen species, gene expression, and enzyme activities of acetyl-CoA carboxylase, malic enzyme, and phosphoenolpyruvate carboxylase.
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
authors.forename: Raoqiong
authors.initials: R
authors.lastname: Che
authors.affiliation: Institute of Chemical Industry, Kunming Metallurgy College, Kunming, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
authors.forename: Jun-Wei
authors.initials: JW
authors.lastname: Xu
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Foreign Languages, Kunming University, Kunming 650200, China.
authors.forename: Huixian
authors.initials: H
authors.lastname: Ma
authors.affiliation: Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, China. Electronic address: xuya_yu@163.com.
authors.forename: Xuya
authors.initials: X
authors.lastname: Yu
mesh_terms: D001578:Benzopyrans / Q000494:pharmacology*; D056804:Biofuels / Q000382:microbiology; D000460:Chlorophyta / Q000502:physiology*; D050356:Lipid Metabolism / Q000187:drug effects*; D008055:Lipids / Q000096:biosynthesis; D014158:Transcription, Genetic / Q000187:drug effects*
chemical_list: D001578:Benzopyrans; D056804:Biofuels; D008055:Lipids; C005023:fulvic acid
keywords: Enzyme activity; Fulvic acid; Gene expression; Lipid; Monoraphidium sp. FXY-10