title: Subtercola lobariae sp. nov., an actinobacterium of the family Microbacteriaceae isolated from the lichen Lobaria retigera.
abstract: An actinobacterium, designated strain 9583bT, was isolated from the lichen Lobaria retigera collected from Jiaozi Snow Mountain, Yunnan Province, China. Cells of strain 9583bT were Gram-stain-positive, aerobic, catalase-positive and oxidase-negative. The strain have a short rod-shaped, irregular morphology, and could grow at the temperature range of 4 to 28 °C. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain 9583bT belonged to the genus Subtercola in the family Microbacteriaceae, and shared highest sequence similarity with the type strains of Subtercola frigoramans and Subtercola boreus (96.8 and 95.6 %, respectively). The peptidoglycan type was B2γ, with diaminobutyric acid as the diagnostic diamino acid. The polar lipids comprised of phosphatidylglycerol, diphosphatidylglycerol, five unidentified glycolipids and three unidentified phospholipids. The respiratory quinone was determined to be MK-10. While the major fatty acids (>5 %) of strain 9583bT were anteiso-C15 : 0, C14 : 0 2-OH and iso-C16 : 0, the 1,1-dimethoxy-alkanes included a-15 : 0 DMA, i-16 : 0 DMA, a-17 : 0 DMA and i-15 : 0 DMA. The genomic DNA G+C content of strain 9583bT was 66.8 mol%. On the basis of the phylogenetic, phenotypic and chemotaxonomic data in this study, strain 9583bT represents a novel species of the genus Subtercola, for which the name Subtercola lobariae sp. nov. is proposed. The type strain is 9583bT (=CGMCC 1.12976T=DSM 103962T).
authors.affiliation: College of Life Sciences, Shandong Normal Univeristy, Jinan 250014, PR China.
authors.forename: Hong-Li
authors.initials: HL
authors.lastname: Si
authors.affiliation: College of Life Sciences, Shandong Normal Univeristy, Jinan 250014, PR China.
authors.forename: Fei-Xiang
authors.initials: FX
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Shandong Normal Univeristy, Jinan 250014, PR China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Shandong Normal Univeristy, Jinan 250014, PR China.
authors.forename: Hong-Shan
authors.initials: HS
authors.lastname: Yue
authors.affiliation: College of Life Sciences, Shandong Normal Univeristy, Jinan 250014, PR China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Shandong Normal Univeristy, Jinan 250014, PR China.
authors.forename: Zun-Tian
authors.initials: ZT
authors.lastname: Zhao
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002473:Cell Wall / Q000737:chemistry; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D008011:Lichens / Q000382:microbiology*; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D014451:Ubiquinone / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C024989:coenzyme Q10