title: Untypeable hepatitis C virus subtypes in Pakistan: A neglected section.
abstract: Diagnostically untypeable subtypes contribute a considerable percent of hepatitis C virus (HCV) subtypes in Pakistan. In the present study, chronically infected HCV patients with known viremia were subjected to HCV genotyping. Among the total retrieved samples, 92.7% (64/69) were found typeable while 7.24% (5/69) were diagnostically untypeable. In conclusion, the presence of large number of untypeable HCV subtypes emphasizes the need of an updated type-specific genotyping assay and consideration of primers for proportionally rare subtypes to minimize the number of untypeable HCV subtypes.
authors.affiliation: 1 Institute of Biotechnology and Genetic Engineering, The University of Agriculture , Peshawar, Pakistan.|2 Center for Biotechnology and Microbiology, University of Swat , Odigram, Pakistan.
authors.forename: Abdul Waheed
authors.initials: AW
authors.lastname: Khan
authors.affiliation: 1 Institute of Biotechnology and Genetic Engineering, The University of Agriculture , Peshawar, Pakistan.
authors.forename: Zeeshan
authors.initials: Z
authors.lastname: Nasim
authors.affiliation: 1 Institute of Biotechnology and Genetic Engineering, The University of Agriculture , Peshawar, Pakistan.
authors.forename: Fazli
authors.initials: F
authors.lastname: Zahir
authors.affiliation: 2 Center for Biotechnology and Microbiology, University of Swat , Odigram, Pakistan.
authors.forename: Shahid
authors.initials: S
authors.lastname: Ali
authors.affiliation: 1 Institute of Biotechnology and Genetic Engineering, The University of Agriculture , Peshawar, Pakistan.
authors.forename: Abid
authors.initials: A
authors.lastname: Ali
authors.affiliation: 1 Institute of Biotechnology and Genetic Engineering, The University of Agriculture , Peshawar, Pakistan.
authors.forename: Aqib
authors.initials: A
authors.lastname: Iqbal
authors.affiliation: 1 Institute of Biotechnology and Genetic Engineering, The University of Agriculture , Peshawar, Pakistan.
authors.forename: Iqbal
authors.initials: I
authors.lastname: Munir
mesh_terms: D005838:Genotype; D016174:Hepacivirus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D006526:Hepatitis C / Q000821:virology*; D006801:Humans; D010154:Pakistan; D012367:RNA, Viral / Q000235:genetics
chemical_list: D012367:RNA, Viral
keywords: Pakistan; genotyping; hepatitis C; untypeable subtypes