title: Mapping of Molecular Structure of the Nanoscale Surface in Bionanoparticles.
abstract: Characterizing the orientation of covalently conjugated proteins on nanoparticles, produced for in vitro and in vivo targeting, though an important feature of such a system, has proved challenging. Although extensive physicochemical characterization of targeting nanoparticles can be addressed in detail, relevant biological characterization of the nanointerface is crucial in order to select suitable nanomaterials for further in vitro or in vivo experiments. In this work, we adopt a methodology using antibody fragments (Fab) conjugated to gold nanoparticles (immunogold) to map the available epitopes on a transferrin grafted silica particle (SiO2-PEG8-Tf) as a proxy methodology to predict nanoparticle biological function, and therefore cellular receptor engagement. Data from the adopted method suggest that, on average, only ∼3.5% of proteins grafted on the SiO2-PEG8-Tf nanoparticle surface have a favorable orientation for recognition by the cellular receptor.
authors.affiliation: Centre for BioNano Interactions, School of Chemistry, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Luciana M
authors.initials: LM
authors.lastname: Herda
authors.affiliation: Centre for BioNano Interactions, School of Chemistry, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Delyan R
authors.initials: DR
authors.lastname: Hristov
authors.affiliation: Centre for BioNano Interactions, School of Chemistry, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Maria Cristina
authors.initials: MC
authors.lastname: Lo Giudice
authors.affiliation: Centre for BioNano Interactions, School of Chemistry, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Ester
authors.identifier: 0000-0001-8870-5280
authors.initials: E
authors.lastname: Polo
authors.affiliation: Centre for BioNano Interactions, School of Chemistry, University College Dublin , Belfield, Dublin 4, Ireland.
authors.forename: Kenneth A
authors.initials: KA
authors.lastname: Dawson
mesh_terms: D000906:Antibodies / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D015394:Molecular Structure; D010316:Particle Size; D012822:Silicon Dioxide / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D000906:Antibodies; D006046:Gold; D012822:Silicon Dioxide