title: Kanamycin detection based on the catalytic ability enhancement of gold nanoparticles.
abstract: In this paper, we demonstrated that kanamycin could enhance the peroxidase-like activity of citrate-capped gold nanoparticles (AuNPs) through two steps: the attachment of kanamycin onto AuNPs through -NH2(on kanamycin) and -COOH (on AuNPs) interactions; and the specifically interaction between glucoside on kanamycin and AuNPs which changes the surface property of AuNPs, and produced •OH radicals and Au3+in the solution, and catalyzed the chromogenic reactions between 3, 3', 5, 5'-tetramethylbenzidine (TMB) and H2O2. Based on this principle, a novel method for kanamycin detection has been developed. This method exhibited high sensitivity and selectivity, as low as 0.1nM kanamycin could be detected with a linear range from 0.1nM to 20nM and 20nM to 300nM, respectively. This method was also successfully applied for the detection of kanamycin content in milk and meat samples.
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China. Electronic address: wangck@ujs.edu.cn.
authors.forename: Chengke
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Qingqing
authors.initials: Q
authors.lastname: Wang
authors.affiliation: College of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, PR China.
authors.forename: Rong
authors.initials: R
authors.lastname: Tan
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis*; D001560:Benzidines / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D002384:Catalysis; D002645:Chickens; D002863:Chromogenic Compounds / Q000737:chemistry; D019343:Citric Acid / Q000737:chemistry; D003124:Colorimetry / Q000379:methods*; D006046:Gold / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000737:chemistry; D007612:Kanamycin / Q000032:analysis*; D057230:Limit of Detection; D008460:Meat / Q000032:analysis*; D053768:Metal Nanoparticles / Q000737:chemistry*; D008892:Milk / Q000737:chemistry*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D001560:Benzidines; D002863:Chromogenic Compounds; D019343:Citric Acid; C021758:3,3',5,5'-tetramethylbenzidine; D007612:Kanamycin; D006046:Gold; D006861:Hydrogen Peroxide
keywords: Biosensor; Catalytic ability; Colorimetric analysis; Gold nanoparticle; Kanamycin