title: Cell morphology, viability, osteocalcin activity, and alkaline phosphatase activity in milled versus unmilled surface of the femoral head.
abstract: To evaluate cellular activity in milled versus unmilled surface of the femoral head in 21 patients who underwent robot-assisted total hip arthroplasty(THA).
  
  
  
  The femoral head of 21 consecutive patients who underwent robot-assisted THA for osteonecrosis was used. 10 cc of trabecular bone from the entire milled surface was obtained using a curette. The same amount of trabecular bone was obtained at least 1 cm away from the milled surface and served as a matched control. Cell morphology, viability, osteocalcin activity, and alkaline phosphatase activity in milled versus unmilled surface were assessed.
  
  
  
  Cell morphology of the milled or unmilled surface was comparable; cells were smaller in the milled surface. Cell viability was a mean of 40% higher in the milled surface (107.4% vs. 67.2%, p<0.001); cell viability at 5 time points was comparable in each group. Osteocalcin activity of cells was slightly higher in the milled surface (1.43 vs. 1.24 ng/ml, p=0.69). Alkaline phosphatase activity of cells was slightly higher in the unmilled surface (150 105 vs. 141 789 U/L, p=0.078).
  
  
  
  The milled and unmilled surfaces of the femoral head were comparable in terms of cell morphology, viability, osteocalcin activity, and alkaline phosphatase activity.
authors.affiliation: Department of Orthopaedic Surgery, College of Medicine, Kyung Hee University, Kyung Hee University Hospital at Gangdong, Seoul, Korea.
authors.forename: Kee Hyung
authors.initials: KH
authors.lastname: Rhyu
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University, Kyunghee-daero, Dongdaemun-gu, Seoul, Korea & Department of Biochemistry and Molecular Biology (BK21 project); Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Kyunghee-daero, Dongdaemun-gu, Seoul, Korea.
authors.forename: Chang Hoon
authors.initials: CH
authors.lastname: Cho
authors.affiliation: Department of Biochemistry and Molecular Biology, School of Medicine, Kyung Hee University, Kyunghee-daero, Dongdaemun-gu, Seoul, Korea & Department of Biochemistry and Molecular Biology (BK21 project); Medical Research Center for Bioreaction to Reactive Oxygen Species and Biomedical Science Institute, School of Medicine, Kyung Hee University, Kyunghee-daero, Dongdaemun-gu, Seoul, Korea.
authors.forename: Kyung Sik
authors.initials: KS
authors.lastname: Yoon
authors.affiliation: Department of Orthopaedic Surgery, College of Medicine, Kyung Hee University, Kyung Hee University Hospital at Gangdong, Seoul, Korea.
authors.forename: Young Soo
authors.initials: YS
authors.lastname: Chun
mesh_terms: D000328:Adult; D000469:Alkaline Phosphatase / Q000378:metabolism*; D019644:Arthroplasty, Replacement, Hip; D002452:Cell Count; D002470:Cell Survival; D005260:Female; D005270:Femur Head / Q000378:metabolism* / Q000473:pathology* / Q000601:surgery; D006801:Humans; D008297:Male; D008875:Middle Aged; D010006:Osteoblasts; D015675:Osteocalcin / Q000378:metabolism*; D010020:Osteonecrosis / Q000601:surgery*
chemical_list: D015675:Osteocalcin; D000469:Alkaline Phosphatase
keywords: alkaline phosphatase; osteoblasts; osteocalcin