title: Measurement of pyridoxal 5'-phosphate, pyridoxal, and 4-pyridoxic acid in the cerebrospinal fluid of children.
abstract: BACKGROUND
  We quantified pyridoxal 5'-phosphate (PLP), pyridoxal (PL), and 4-pyridoxic acid (PA) in the cerebrospinal fluid (CSF) of children and to investigate the effect of age, sex, epilepsy, and anti-epileptic drug (AED) therapy on these vitamers.
  
  
  METHODS
  CSF samples prospectively collected from 116 pediatric patients were analyzed. PLP, PL, and PA were measured using high-performance liquid chromatography with fluorescence detection, using pre-column derivatization by semicarbazide. Effects of age, sex, epilepsy, and AEDs on these vitamers and the PLP/PL ratio were evaluated using multiple linear regression models.
  
  
  RESULTS
  The PLP, PL, and PA concentrations were correlated negatively with age and the PLP/PL ratio was correlated positively with age. Multiple regression analysis revealed that the presence of epilepsy was associated with lower PLP concentrations and PLP/PL ratios but sex and AED therapy had no influence on these values. The observed ranges of these vitamers in epileptic and non-epileptic patients were demonstrated.
  
  
  CONCLUSIONS
  We showed the age dependence of PLP and PL in CSF from pediatric patients. Epileptic patients had lower PLP concentrations and PLP/PL ratios than non-epileptic patients, but it is unknown whether this is the cause, or a result, of epilepsy.
authors.affiliation: Department of Child Neurology, Okayama University Hospital, Okayama, Japan. Electronic address: takiyama@okayama-u.ac.jp.
authors.forename: Tomoyuki
authors.initials: T
authors.lastname: Akiyama
authors.affiliation: Department of Child Neurology, Okayama University Hospital, Okayama, Japan.
authors.forename: Mari
authors.initials: M
authors.lastname: Akiyama
authors.affiliation: Department of Child Neurology, Okayama University Hospital, Okayama, Japan.
authors.forename: Yumiko
authors.initials: Y
authors.lastname: Hayashi
authors.affiliation: Department of Child Neurology, Okayama University Hospital, Okayama, Japan; Department of Child Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Shibata
authors.affiliation: Department of Child Neurology, Okayama University Hospital, Okayama, Japan; Department of Child Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Yoshiyuki
authors.initials: Y
authors.lastname: Hanaoka
authors.affiliation: Department of Pediatrics, Kameda Medical Center, Chiba, Japan.
authors.forename: Soichiro
authors.initials: S
authors.lastname: Toda
authors.affiliation: Department of Pediatrics, NHO Shizuoka Institute of Epilepsy and Neurological Disorders, Shizuoka, Japan.
authors.forename: Katsumi
authors.initials: K
authors.lastname: Imai
authors.affiliation: Division of Neurology, Saitama Children's Medical Center, Saitama, Japan.
authors.forename: Shin-Ichiro
authors.initials: SI
authors.lastname: Hamano
authors.affiliation: Department of Child Neurology, Seirei Hamamatsu General Hospital, Hamamatsu, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Okanishi
authors.affiliation: Department of Child Neurology, Okayama University Hospital, Okayama, Japan; Department of Child Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Harumi
authors.initials: H
authors.lastname: Yoshinaga
authors.affiliation: Department of Child Neurology, Okayama University Hospital, Okayama, Japan; Department of Child Neurology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Katsuhiro
authors.initials: K
authors.lastname: Kobayashi
mesh_terms: D000367:Age Factors; D002648:Child; D002851:Chromatography, High Pressure Liquid; D004827:Epilepsy / Q000134:cerebrospinal fluid* / Q000209:etiology; D005260:Female; D006801:Humans; D016014:Linear Models; D008297:Male; D011446:Prospective Studies; D011730:Pyridoxal / Q000134:cerebrospinal fluid*; D011732:Pyridoxal Phosphate / Q000134:cerebrospinal fluid*; D011735:Pyridoxic Acid / Q000134:cerebrospinal fluid*; D012737:Sex Factors
chemical_list: D011730:Pyridoxal; D011735:Pyridoxic Acid; D011732:Pyridoxal Phosphate
keywords: 4-pyridoxic acid; Epilepsy; Fluorescence detection; Liquid chromatography; Pyridoxal phosphate; Vitamin B6