title: Proteins that interact with calgranulin B in the human colon cancer cell line HCT-116.
abstract: Calgranulin B is released from immune cells and can be internalized into colon cancer cells to prevent proliferation. The present study aimed to identify proteins that interact with calgranulin B to suppress the proliferation of colon cancer cells, and to obtain information on the underlying anti-tumor mechanism(s) of calgranulin B. Calgranulin B expression was induced in colon cancer cell line HCT-116 by infection with calgranulin B-FLAG expressing lentivirus, and it led to a significant suppression of cell proliferation. Proteins that interacted with calgranulin B were obtained by immunoprecipitation using whole homogenate of lentivirus-infected HCT-116 cells which expressing calgranulin B-FLAG, and identified using liquid chromatography-mass spectrometry/mass spectrometry analysis. A total of 454 proteins were identified that potentially interact with calgranulin B, and most identified proteins were associated with RNA processing, post-transcriptional modifications and the EIF2 signaling pathway. Direct interaction of calgranulin B with flotillin-1, dynein intermediate chain 1, and CD59 glycoprotein has been confirmed, and the molecules N-myc proto-oncogene protein, rapamycin-insensitive companion of mTOR, and myc proto-oncogene protein were shown to regulate calgranulin B-interacting proteins. Our results provide new insight and useful information to explain the possible mechanism(s) underlying the role of calgranulin B as an anti-tumor effector in colon cancer cells.
authors.affiliation: Department of System Cancer Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang, Korea.
authors.forename: Jae Kyung
authors.initials: JK
authors.lastname: Myung
authors.affiliation: Department of Radiation Oncology, Soonchunhyang University College of Medicine, Cheonan, Korea.
authors.forename: Seung-Gu
authors.initials: SG
authors.lastname: Yeo
authors.affiliation: Colorectal Cancer Branch, Research Institute, National Cancer Center, Goyang, Korea.|Omics Core, Research Institute, National Cancer Center, Goyang, Korea.
authors.forename: Kyung Hee
authors.initials: KH
authors.lastname: Kim
authors.affiliation: Colorectal Cancer Branch, Research Institute, National Cancer Center, Goyang, Korea.|Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: Kwang-Soo
authors.initials: KS
authors.lastname: Baek
authors.affiliation: Department of System Cancer Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang, Korea.|Cancer Cell and Molecular Biology Branch, Research Institute, National Cancer Center, Goyang, Korea.
authors.forename: Daye
authors.initials: D
authors.lastname: Shin
authors.affiliation: Department of System Cancer Science, Graduate School of Cancer Science and Policy, National Cancer Center, Goyang, Korea.|Cancer Cell and Molecular Biology Branch, Research Institute, National Cancer Center, Goyang, Korea.
authors.forename: Jong Heon
authors.initials: JH
authors.lastname: Kim
authors.affiliation: Department of Genetic Engineering, Sungkyunkwan University, Suwon, Korea.
authors.forename: Jae Youl
authors.initials: JY
authors.lastname: Cho
authors.affiliation: Colorectal Cancer Branch, Research Institute, National Cancer Center, Goyang, Korea.
authors.forename: Byong Chul
authors.initials: BC
authors.lastname: Yoo
mesh_terms: D018957:CD59 Antigens / Q000378:metabolism; D040502:Calgranulin B / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D002851:Chromatography, High Pressure Liquid; D003110:Colonic Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D056445:Cytoplasmic Dyneins / Q000378:metabolism; D045325:HCT116 Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism; D000071447:N-Myc Proto-Oncogene Protein / Q000378:metabolism; D060066:Protein Interaction Maps; D040901:Proteomics / Q000379:methods; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc / Q000378:metabolism; D015398:Signal Transduction; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D013997:Time Factors; D014162:Transfection
chemical_list: D018957:CD59 Antigens; D040502:Calgranulin B; C000715470:MAS1 protein, human; C489427:MYC protein, human; C459801:MYCN protein, human; D008565:Membrane Proteins; D000071447:N-Myc Proto-Oncogene Protein; D000090063:Proto-Oncogene Mas; D016271:Proto-Oncogene Proteins c-myc; C106354:flotillins; C051590:CD59 protein, human; C546842:MTOR protein, human; D058570:TOR Serine-Threonine Kinases; D056445:Cytoplasmic Dyneins
keywords: HCT-116; S100A9; anti-tumor effect; calgranulin B; colon cancer