title: Chemical Fingerprint Analysis and Quantitative Analysis of Rosa rugosa by UPLC-DAD.
abstract: A method based on ultra performance liquid chromatography with a diode array detector (UPLC-DAD) was developed for quantitative analysis of five active compounds and chemical fingerprint analysis ofRosa rugosa. Ten batches ofR. rugosacollected from different plantations in the Xinjiang region of China were used to establish the fingerprint. The feasibility and advantages of the used UPLC fingerprint were verified for its similarity evaluation by systematically comparing chromatograms with professional analytical software recommended by State Food and Drug Administration (SFDA) of China. In quantitative analysis, the five compounds showed good regression (R² = 0.9995) within the test ranges, and the recovery of the method was in the range of 94.2%-103.8%. The similarities of liquid chromatography fingerprints of 10 batches ofR. rugosawere more than 0.981. The developed UPLC fingerprint method is simple, reliable, and validated for the quality control and identification ofR. rugosa. Additionally, simultaneous quantification of five major bioactive ingredients in theR. rugosasamples was conducted to interpret the consistency of the quality test. The results indicated that the UPLC fingerprint, as a characteristic distinguishing method combining similarity evaluation and quantification analysis, can be successfully used to assess the quality and to identify the authenticity ofR. rugosa.
authors.affiliation: Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. sanam0405@163.com.|Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. sanam0405@163.com.|University of Chinese Academy of Sciences, Beijing 100049, China. sanam0405@163.com.
authors.forename: Sanawar
authors.initials: S
authors.lastname: Mansur
authors.affiliation: Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. rahima@ms.xjb.ac.cn.|University of Chinese Academy of Sciences, Beijing 100049, China. rahima@ms.xjb.ac.cn.
authors.forename: Rahima
authors.initials: R
authors.lastname: Abdulla
authors.affiliation: Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. amatjanayupbig@hotmail.com.|Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. amatjanayupbig@hotmail.com.
authors.forename: Amatjan
authors.initials: A
authors.lastname: Ayupbec
authors.affiliation: Key Laboratory of Xinjiang Indigenous Medicinal Plants Resource Utilization, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. haji@ms.xjb.ac.cn.|Key Laboratory of Plant Resources and Chemistry in Arid Regions, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China. haji@ms.xjb.ac.cn.
authors.forename: Haji Akbar
authors.initials: HA
authors.lastname: Aisa
mesh_terms: D002681:China; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods / Q000592:standards*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000592:standards*; D004610:Ellagic Acid / Q000302:isolation & purification / Q000592:standards; D005707:Gallic Acid / Q000302:isolation & purification / Q000592:standards; D006801:Humans; D044949:Kaempferols / Q000302:isolation & purification / Q000592:standards; D011786:Quality Control; D011794:Quercetin / Q000031:analogs & derivatives / Q000302:isolation & purification / Q000592:standards; D015203:Reproducibility of Results; D027826:Rosa / Q000737:chemistry*; D012680:Sensitivity and Specificity
chemical_list: D004365:Drugs, Chinese Herbal; D044949:Kaempferols; D004610:Ellagic Acid; C064309:kaempferol 3-O-sophoroside; D005707:Gallic Acid; C021304:hyperoside; D011794:Quercetin; C001579:astragalin
keywords: Rosa rugosa; UPLC-DAD; fingerprint; quality control