title: Hydroxypropyl cellulose supplementation in vitrification solutions: a prospective study with donor oocytes.
abstract: OBJECTIVE
  Hydroxypropyl cellulose (HPC), a polysaccharide that forms a viscous gel under low temperatures, is a promising substitute of the blood-derived macromolecules traditionally used in cryopreservation solutions. The performance of a protein-free, fully synthetic set of vitrification and warming solutions was assessed in a matched pair analysis with donor oocytes.
  
  
  METHODS
  A prospective study including 219 donor MII oocytes was carried out, comparing the laboratory outcomes of oocytes vitrified with HPC-based solutions and their fresh counterparts. The primary performance endpoint was the fertilization rate. Secondary parameters assessed were embryo quality on days 2 and 3.
  
  
  RESULTS
  70/73 (95.9%) vitrified MII oocytes exhibited morphologic survival 2 h post-warming, with 49 (70.0%) presented normal fertilization, compared to 105 of 146 (71.9%) MII fresh oocytes. Similar embryo quality was observed in both groups. A total of 18 embryos implanted, out of 38 embryos transferred (47.3%), resulting in 13 newborns.
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.|Seville Engineering School, University of Seville, Camino de los Descubrimientos s/n, 41092, Seville, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Gallardo
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: María
authors.initials: M
authors.lastname: Hebles
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Migueles
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Dorado
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Aguilera
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Mercedes
authors.initials: M
authors.lastname: González
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Paloma
authors.initials: P
authors.lastname: Piqueras
authors.affiliation: Seville Engineering School, University of Seville, Camino de los Descubrimientos s/n, 41092, Seville, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Lucas
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Montero
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Pascual
authors.initials: P
authors.lastname: Sánchez-Martín
authors.affiliation: Clínica Ginemed, C/ Farmacéutico Murillo Herrera n 3-5, 41010, Seville, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Sánchez-Martín
authors.affiliation: Seville Engineering School, University of Seville, Camino de los Descubrimientos s/n, 41092, Seville, Spain. risco@us.es.|National Accelerators Centre, Calle Thomas Alva Edison, 7, 41092, Seville, Spain. risco@us.es.
authors.forename: Ramón
authors.initials: R
authors.lastname: Risco
mesh_terms: D002482:Cellulose / Q000008:administration & dosage / Q000031:analogs & derivatives; D015925:Cryopreservation / Q000379:methods; D004624:Embryo Transfer / Q000379:methods*; D047108:Embryonic Development / Q000187:drug effects; D005260:Female; D005307:Fertilization in Vitro / Q000379:methods*; D006801:Humans; D009865:Oocytes / Q000187:drug effects* / Q000254:growth & development; D011247:Pregnancy; D018873:Pregnancy Rate; D014019:Tissue Donors; D058989:Vitrification / Q000187:drug effects*
chemical_list: D002482:Cellulose; C008079:hydroxypropylcellulose
keywords: Hydroxypropyl cellulose; Oocyte; Protein supplementation; Serum free; Vitrification