title: Initial development of Babesia ovata in the tick midgut.
abstract: The initial development of Babesia ovata in the midgut of the vector tick Haemaphysalis longicornis has been demonstrated through in vitro and in vivo studies. Although the research on the partial developmental cycles of B. ovata in the tick midgut was performed in our previous study by using ticks fed on experimentally B. ovata-infected cattle, detailed information on the developmental stages of B. ovata in H. longicornis was limited. This report describes the sequential development of stages of B. ovata in an in vitro study using B. ovata-infected erythrocytes and tick midgut contents. The in vivo study also confirmed the developmental stages in the midgut contents of artificially B. ovata-infected ticks. In this observation, we have recognized the distinct forms of B. ovata developmental stages in the tick midgut; the aggregation forms and ray bodies with shorter spikes and light-stained cytoplasm were shown by Giemsa staining. The similarities and differences of the stages as compared to previous reports have been discussed.
authors.affiliation: Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Maeda
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan. Electronic address: htakeshi@med.kitasato-u.ac.jp.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Hatta
authors.affiliation: Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh 2202, Bangladesh.
authors.forename: M Abdul
authors.initials: MA
authors.lastname: Alim
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Daigo
authors.initials: D
authors.lastname: Tsubokawa
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Fusako
authors.initials: F
authors.lastname: Mikami
authors.affiliation: Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan.
authors.forename: Kodai
authors.initials: K
authors.lastname: Kusakisako
authors.affiliation: Laboratory of International Prevention of Epidemics, Graduate School of Life and Environmental Sciences, Osaka Prefecture University, Gakuen-cho, Nakaku, Sakai, Osaka 599-8531, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Matsubayashi
authors.affiliation: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, Hokkaido 080-8555, Japan.
authors.forename: Rika
authors.initials: R
authors.lastname: Umemiya-Shirafuji
authors.affiliation: Department of Parasitology, Kitasato University School of Medicine, 1-15-1 Kitasato, Kitasato, Minami, Sagamihara, Kanagawa 252-0374, Japan.
authors.forename: Naotoshi
authors.initials: N
authors.lastname: Tsuji
authors.affiliation: Laboratory of Infectious Diseases, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, 1-21-24 Korimoto, Kagoshima 890-0065, Japan; Department of Pathological and Preventive Veterinary Science, The United Graduate School of Veterinary Science, Yamaguchi University, Yoshida, Yamaguchi 753-8515, Japan. Electronic address: k6199431@kadai.jp.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Tanaka
mesh_terms: D000818:Animals; D001403:Babesia / Q000166:cytology / Q000254:growth & development*; D002417:Cattle; D004064:Digestive System / Q000469:parasitology; D004912:Erythrocytes / Q000469:parasitology; D008018:Life Cycle Stages; D013987:Ticks / Q000469:parasitology*
keywords: Artificial induction; Babesia ovata; Midgut contents; Midgut stage