title: Antibiotic Spider Silk: Site-Specific Functionalization of Recombinant Spider Silk Using "Click" Chemistry.
abstract: In a new, versatile approach to fun-ction-alizing recombinant spider silk, L-azidohomoalanine is introduced residue-specifically in the minispidroin protein 4RepCT through expression in an E. coli methionine auxotroph. Both fluorophores and the antibiotic levofloxacin are attached to this bio-orthogonal amino acid using copper-catalyzed click chemistry, either before or after the silk fibers are self-assembled.
authors.affiliation: School of Chemistry, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: David
authors.initials: D
authors.lastname: Harvey
authors.affiliation: School of Chemistry, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Philip
authors.initials: P
authors.lastname: Bardelang
authors.affiliation: School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Sara L
authors.initials: SL
authors.lastname: Goodacre
authors.affiliation: School of Life Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Alan
authors.initials: A
authors.lastname: Cockayne
authors.affiliation: School of Chemistry, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK.
authors.forename: Neil R
authors.initials: NR
authors.lastname: Thomas
keywords: antibiotic; click chemistry; self-assembly; spider silk; unnatural (noncanonical) amino acid mutagenesis