title: Mesenchymal stem cells ameliorate B-cell-mediated immune responses and increase IL-10-expressing regulatory B cells in an EBI3-dependent manner.
abstract: Effector B cells are central contributors to the development of autoimmune disease by activating autoreactive T cells, producing pro-inflammatory cytokines and organizing ectopic lymphoid tissue. Conversely, IL-10-producing regulatory B (Breg) cells have pivotal roles in maintaining immunological tolerance and restraining excessive inflammation in autoinflammatory disease. Thus, regulating the equilibrium between antibody-producing effector B cells and Bregcells is critical for the treatment of autoimmune disease. In this study, we investigated the effect of human palatine tonsil-derived mesenchymal stem cells (T-MSCs) on estradiol (E2)-induced B-cell responses in vivo and in vitro. Transplantation of T-MSC into E2-treated mice alleviated B-cell-mediated immune responses and increased the population of IL-10-producing Bregcells. T-MSCs regulated the B-cell populations by producing Epstein-Barr virus (EBV)-induced 3 (EBI3), one of the two subunits of IL-35 that is the well-known inducer of Bregcells. We demonstrate a critical role of EBI3 (IL-35) in vitro by depleting EBI3 in T-MSCs and by adding exogenous IL-35 to the culture system. Taken together, our data suggest that IL-35-secreting MSCs may become an attractive therapeutic to treat B-cell-mediated autoimmune diseases via expanding Bregcells.Cellular & Molecular Immunology advance online publication, 2 January 2017; doi:10.1038/cmi.2016.59.
authors.affiliation: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
authors.forename: Kyung-Ah
authors.initials: KA
authors.lastname: Cho
authors.affiliation: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
authors.forename: Jun-Kyu
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
authors.forename: Yu-Hee
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
authors.forename: Minhwa
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Microbiology, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
authors.forename: So-Youn
authors.initials: SY
authors.lastname: Woo
authors.affiliation: Department of Pediatrics, School of Medicine, Ewha Womans University, Seoul 07985, Republic of Korea.
authors.forename: Kyung-Ha
authors.initials: KH
authors.lastname: Ryu