title: Thin-Film Transistor-Based Biosensors for Determining Stoichiometry of Biochemical Reactions.
abstract: The enzyme kinetic in a biochemical reaction is critical to scientific research and drug discovery but can hardly be determined experimentally from enzyme assays. In this work, a charge-current transducer (a transistor) is proposed to evaluate the status of biochemical reaction by monitoring the electrical charge changes. Using the malate-aspartate shuttle as an example, a thin-film transistor (TFT)-based biosensor with an extended gold pad is demonstrated to detect the biochemical reaction between NADH and NAD+. The drain current change indicates the status of chemical equilibrium and stoichiometry.
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, Taiwan.
authors.forename: Yi-Wen
authors.initials: YW
authors.lastname: Wang
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, Taiwan.
authors.forename: Ting-Yang
authors.initials: TY
authors.lastname: Chen
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, Taiwan.
authors.forename: Tsung-Han
authors.initials: TH
authors.lastname: Yang
authors.affiliation: Graduate Institute of Biomedical Engineering, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Cheng-Chung
authors.initials: CC
authors.lastname: Chang
authors.affiliation: Department of Otolaryngology, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan.|Research Center for Developmental Biology and Regenerative Medicine, National Taiwan UniversityTaipei, Taiwan.
authors.forename: Tsung-Lin
authors.initials: TL
authors.lastname: Yang
authors.affiliation: Materials Science and Engineering Program, University of California San Diego, La Jolla, California, United States of America.|Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, California, United States of America.
authors.forename: Yu-Hwa
authors.initials: YH
authors.lastname: Lo
authors.affiliation: Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, Taiwan.|Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan.
authors.forename: Jian-Jang
authors.identifier: http://orcid.org/0000-0002-5761-2177
authors.initials: JJ
authors.lastname: Huang
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation*; D004553:Electric Conductivity; D007700:Kinetics; D009243:NAD / Q000378:metabolism; D062907:Oxaloacetic Acid / Q000378:metabolism; D014173:Transistors, Electronic
chemical_list: D009243:NAD; D062907:Oxaloacetic Acid