title: Exhaled breath condensate analysis from intubated newborns by nano-HPLC coupled to high resolution MS.
abstract: Invasiveness of examination and therapy methods is a serious problem for intensive care and nursing of premature infants. Exhaled breath condensate (EBC) is the most attractive biofluid for non-invasive methods development in neonatology for monitoring the status of intubated infants. The aim of the study was to propose an approach for EBC sampling and analysis from mechanically ventilated neonates. EBC collection system with good reproducibility of sampling was demonstrated. Discovery-based proteomic and metabolomic studies were performed using nano-HPLC coupled to high resolution MS. Label-free semi-quantitative data were compared for intubated neonates with congenital pneumonia (12 infants) and left-sided congenital diaphragmatic hernia (12 infants) in order to define disease-specific features. Totally 119 proteins and 164 metabolites were found. A number of proteins and metabolites that can act as potential biomarkers of respiratory diseases were proposed and require further validation.
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia; Moscow Institute of Physics and Technology, Moscow, Russia. Electronic address: alex.kononikhin@gmail.com.
authors.forename: A S
authors.initials: AS
authors.lastname: Kononikhin
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; Moscow Institute of Physics and Technology, Moscow, Russia.
authors.forename: N L
authors.initials: NL
authors.lastname: Starodubtseva
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: V V
authors.initials: VV
authors.lastname: Chagovets
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: A Y
authors.initials: AY
authors.lastname: Ryndin
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Burov
authors.affiliation: Moscow Institute of Physics and Technology, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: I A
authors.initials: IA
authors.lastname: Popov
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Bugrova
authors.affiliation: Moscow Institute of Physics and Technology, Moscow, Russia.
authors.forename: R A
authors.initials: RA
authors.lastname: Dautov
authors.affiliation: Moscow Institute of Physics and Technology, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia.
authors.forename: A O
authors.initials: AO
authors.lastname: Tokareva
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: Y L
authors.initials: YL
authors.lastname: Podurovskaya
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Ionov
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Frankevich
authors.affiliation: Moscow Institute of Physics and Technology, Moscow, Russia; Emanuel Institute for Biochemical Physics, Russian Academy of Sciences, Moscow, Russia; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia; Skolkovo Institute of Science and Technology, Skolkovo, Russia. Electronic address: ennikolaev@rambler.ru.
authors.forename: E N
authors.initials: EN
authors.lastname: Nikolaev
authors.affiliation: V. I. Kulakov Research Center for Obstetrics, Gynecology and Perinatology, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
authors.forename: G T
authors.initials: GT
authors.lastname: Sukhikh
mesh_terms: D015415:Biomarkers / Q000032:analysis; D001944:Breath Tests / Q000295:instrumentation*; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation*; D004867:Equipment Design; D005260:Female; D065630:Hernias, Diaphragmatic, Congenital / Q000175:diagnosis* / Q000378:metabolism; D006801:Humans; D007231:Infant, Newborn; D008297:Male; D013058:Mass Spectrometry / Q000295:instrumentation*; D055442:Metabolome; D055432:Metabolomics / Q000295:instrumentation; D011014:Pneumonia / Q000175:diagnosis* / Q000378:metabolism; D011506:Proteins / Q000032:analysis; D040901:Proteomics / Q000295:instrumentation; D015203:Reproducibility of Results
chemical_list: D015415:Biomarkers; D011506:Proteins
keywords: Exhaled breath condensate (EBC); HPLC; Mass spectrometry; Metabolomics; Newborns; Pneumonia; Proteomic; Respiratory diseases