title: Novel Peptide with Specific Calcium-Binding Capacity from Schizochytrium sp. Protein Hydrolysates and Calcium Bioavailability in Caco-2 Cells.
abstract: Peptide-calcium can probably be a suitable supplement to improve calcium absorption in the human body. In this study, a specific peptide Phe-Tyr (FY) with calcium-binding capacity was purified fromSchizochytriumsp. protein hydrolysates through gel filtration chromatography and reversed phase HPLC. The calcium-binding capacity of FY reached 128.77 ± 2.57 μg/mg. Results of ultraviolet spectroscopy, fluorescence spectroscopy, and infrared spectroscopy showed that carboxyl groups, amino groups, and amido groups were the major chelating sites. FY-Ca exhibited excellent thermal stability and solubility, which were beneficial to be absorbed and transported in the basic intestinal tract of the human body. Moreover, the calcium bioavailability in Caco-2 cells showed that FY-Ca could enhance calcium uptake efficiency by more than three times when compared with CaCl₂, and protect calcium ions against dietary inhibitors, such as tannic acid, oxalate, phytate, and Zn2+. Our findings further the progress of algae-based peptide-calcium, suggesting that FY-Ca has the potential to be developed as functionally nutraceutical additives.
authors.affiliation: The Key Lab of Analysis and Detection Technology for Food Safety of the MOE, College of Chemistry, Fuzhou University, Fuzhou 350108, China. caixx_0123@163.com.|College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China. caixx_0123@163.com.
authors.forename: Xixi
authors.initials: X
authors.lastname: Cai
authors.affiliation: College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China. kathleen369@163.com.
authors.forename: Jiaping
authors.initials: J
authors.lastname: Lin
authors.affiliation: College of Biological Science and Technology, Fuzhou University, Fuzhou 350108, China. shywang@fzu.edu.cn.
authors.forename: Shaoyun
authors.initials: S
authors.lastname: Wang
mesh_terms: D001682:Biological Availability; D001692:Biological Transport / Q000502:physiology; D018938:Caco-2 Cells; D002118:Calcium / Q000378:metabolism*; D045744:Cell Line, Tumor; D002614:Chelating Agents / Q000737:chemistry / Q000494:pharmacology; D019587:Dietary Supplements; D006801:Humans; D007477:Ions / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology*; D011492:Protein Hydrolysates / Q000737:chemistry*; D012995:Solubility; D058009:Stramenopiles / Q000737:chemistry*
chemical_list: D002614:Chelating Agents; D007477:Ions; D010455:Peptides; D011492:Protein Hydrolysates; D002118:Calcium
keywords: Schizochytrium sp.; bioavailability; calcium-binding peptide; protein hydrolysate; structure