title: Functionalized silk fibroin dressing with topical bioactive insulin release for accelerated chronic wound healing.
abstract: The healing of chronic wounds remains a key challenge in regenerative medicine. To promote wound healing, a bioactive wound dressing is required. In this study, a functionalized silk fibroin dressing with topical bioactive insulin release was prepared for the treatment of chronic wounds. For this purpose, insulin-encapsulated silk fibroin (SF) microparticles were prepared by coaxial electrospraying of aqueous SF solution under mild processing conditions. Insulin was successfully encapsulated in the inner layer of SF microparticles, providing a sustained insulin release for up to 28days. It was found that the insulin released from the microparticles could maintain original molecular conformation. Moreover, the cell migration assay based on human keratinocyte and endothelial cells confirmed that the insulin released from SF microparticles retained its native bioactivity. Furthermore, the insulin-encapsulated microparticles were loaded into a SF sponge, functioned as a bioactive wound dressing, and the in vivo therapeutic effect of the sponge dressing was evaluated on dorsal full thickness wounds of diabetic Sprague-Dawley rats. The results showed that an insulin-functionalized SF dressing accelerated wound closure, collagen deposition and vascularization, thus, significantly promoting wound healing. The insulin-functionalized SF dressing provides new treatment options for chronic wounds.
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Burn and Plastic Surgery, School of Medicine Affiliated Ruijin Hospital, Shanghai JiaoTong University, Shanghai 200025, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Burn and Plastic Surgery, School of Medicine Affiliated Ruijin Hospital, Shanghai JiaoTong University, Shanghai 200025, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Textile Science and Engineering, Wuhan Textile University, Wuhan 430200, China.
authors.forename: Renchuan
authors.initials: R
authors.lastname: You
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Qu
authors.affiliation: National Engineering Laboratory for Modern Silk, College of Textile and Clothing Engineering, Soochow University, No. 199 Ren'ai Road, Industrial Park, Suzhou 215123, China. Electronic address: mzli@suda.edu.cn.
authors.forename: Mingzhong
authors.initials: M
authors.lastname: Li
mesh_terms: D000818:Animals; D001458:Bandages; D002460:Cell Line; D002465:Cell Movement / Q000187:drug effects; D002942:Circular Dichroism; D003921:Diabetes Mellitus, Experimental / Q000139:chemically induced / Q000188:drug therapy; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D005349:Fibroins / Q000737:chemistry*; D016650:Fluorescein-5-isothiocyanate / Q000737:chemistry; D006801:Humans; D007150:Immunohistochemistry; D007328:Insulin / Q000737:chemistry* / Q000494:pharmacology / Q000627:therapeutic use; D008297:Male; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D019408:Platelet Endothelial Cell Adhesion Molecule-1 / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D017550:Spectroscopy, Fourier Transform Infrared; D014945:Wound Healing / Q000187:drug effects
chemical_list: D004337:Drug Carriers; D007328:Insulin; D019408:Platelet Endothelial Cell Adhesion Molecule-1; D005349:Fibroins; D016650:Fluorescein-5-isothiocyanate
keywords: Dressings; Insulin release; Microparticles; Silk fibroin; Wound healing