title: RELM-β promotes human pulmonary artery smooth muscle cell proliferation via FAK-stimulated surviving.
abstract: Resistin-like molecule-β (RELM-β), focal adhesion kinase (FAK), and survivin may be involved in the proliferation of cultured human pulmonary artery smooth muscle cells (HPAMSCs), which is involved in pulmonary hypertension. HPAMSCs were treated with human recombinant RELM-β (rhRELM-β). siRNAs against FAK and survivin were transfected into cultured HPASMCs. Expression of FAK and survivin were examined by RT-PCR and western blot. Immunofluorescence was used to localize FAK. Flow cytometry was used to examine cell cycle distribution and cell death. Compared to the control group, all rhRELM-β-treated groups demonstrated significant increases in the expression of FAK and survivin (P<0.05). rhRELM-β significantly increased the proportion of HPASMCs in the S phase and decreased the proportion in G0/G1. FAK siRNA down-regulated survivin expression while survivin siRNA did not affect FAK expression. FAK siRNA effectively inhibited FAK and survivin expression in RELM-β-treated HPASMCs and partially suppressed cell proliferation. RELM-β promoted HPASMC proliferation and upregulated FAK and survivin expression. In conclusion, results suggested that FAK is upstream of survivin in the signaling pathway mediating cell proliferation. FAK seems to be important in RELM-β-induced HPASMC proliferation, partially by upregulating survivin expression.
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China. Electronic address: lclmd@sina.com.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Lin
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Li
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Lun
authors.initials: L
authors.lastname: Li
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Li
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Tang
authors.affiliation: Respiratory Department of the Second Hospital of Yueyang, Hunan Normal University, Yueyang, Hunan 414000, China.
authors.forename: Lifu
authors.initials: L
authors.lastname: Wu
mesh_terms: D002453:Cell Cycle; D049109:Cell Proliferation; D002478:Cells, Cultured; D051416:Focal Adhesion Protein-Tyrosine Kinases / Q000378:metabolism*; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000235:genetics / Q000378:metabolism; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology*; D009131:Muscle, Smooth, Vascular / Q000166:cytology*; D032389:Myocytes, Smooth Muscle / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D011651:Pulmonary Artery / Q000166:cytology; D000077022:Survivin
chemical_list: C107489:BIRC5 protein, human; D051034:Inhibitor of Apoptosis Proteins; D036341:Intercellular Signaling Peptides and Proteins; C486574:RETNLB protein, human; D000077022:Survivin; D051416:Focal Adhesion Protein-Tyrosine Kinases
keywords: FAK; Proliferation; Pulmonary artery smooth muscle cells; Resistin-like molecule-β; Survivin