title: Vitamin C Protects Chondrocytes against Monosodium Iodoacetate-Induced Osteoarthritis by Multiple Pathways.
abstract: Osteoarthritis (OA) is the most prevalent joint disease. Dietary intake of vitamin C relates to a reduction in cartilage loss and OA. This study examined the efficacy of vitamin C to prevent OA with the in vitro chondrosarcoma cell line (SW1353) and the in vivo monosodium iodoacetate (MIA)-induced OA rat. Results demonstrated that, in SW1353 cells, treatment with 5 μM MIA inhibited cell growth and increased oxidative stress, apoptosis, and proteoglycan loss. In addition, the expression levels of the pro-inflammatory cytokines IL-6, IL-17A, and TNF-α and matrix metalloproteinases (MMPs) MMP-1, MMP-3, and MMP-13 were increased. All of these MIA-induced changes could be prevented with treatment of 100 μM vitamin C. In an animal model, intra-articular injection of MIA-induced cartilage degradation resembled the pathological changes of OA, and treatment of vitamin C could lessen these changes. Unexpectedly, vitamin C's effects did not strengthen with the increasing dosage, while the 100 mg/kg dosage was more efficient than the 200 or 300 mg/kg dosages. Vitamin C possessed multiple capacities for prevention of OA progress, including a decrease in apoptosis and in the expression of pro-inflammatory cytokines and MMPs in addition to the well-known antioxidation.
authors.affiliation: Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. cprong@gmail.com.|Department of Biochemistry, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. cprong@gmail.com.
authors.forename: Pu-Rong
authors.initials: PR
authors.lastname: Chiu
authors.affiliation: Department of Biochemistry, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. chingshouhu@gmail.com.
authors.forename: Yu-Chen
authors.initials: YC
authors.lastname: Hu
authors.affiliation: Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. huangtavia@gmail.com.|Department of Biochemistry, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. huangtavia@gmail.com.
authors.forename: Tzu-Ching
authors.initials: TC
authors.lastname: Huang
authors.affiliation: Department of Biochemistry, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. hsiehbs@gmail.com.
authors.forename: Bau-Shan
authors.initials: BS
authors.lastname: Hsieh
authors.affiliation: Department of Biochemistry, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. masakykaou@hotmail.com.
authors.forename: Jou-Pei
authors.initials: JP
authors.lastname: Yeh
authors.affiliation: Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan. Chenghl.tanya@gmail.com.
authors.forename: Hsiao-Ling
authors.initials: HL
authors.lastname: Cheng
authors.affiliation: Department of Medical Laboratory Science and Biotechnology, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. lewehu@cc.kmu.edu.tw.
authors.forename: Li-Wen
authors.initials: LW
authors.lastname: Huang
authors.affiliation: Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Chang.KeeLung@msa.hinet.net.|Department of Biochemistry, School of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 80708, Taiwan. Chang.KeeLung@msa.hinet.net.|Institute of Medical Science and Technology, College of Sciences, National Sun Yat-sen University, Kaohsiung 80424, Taiwan. Chang.KeeLung@msa.hinet.net.|Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung 80756, Taiwan. Chang.KeeLung@msa.hinet.net.
authors.forename: Kee-Lung
authors.initials: KL
authors.lastname: Chang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001205:Ascorbic Acid / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D045744:Cell Line, Tumor; D019902:Chondrocytes / Q000187:drug effects*; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D019807:Iodoacetic Acid / Q000633:toxicity; D008297:Male; D020782:Matrix Metalloproteinases / Q000378:metabolism; D010003:Osteoarthritis / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D018384:Oxidative Stress; D011509:Proteoglycans / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D014815:Vitamins / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D016207:Cytokines; D011509:Proteoglycans; D014815:Vitamins; D020782:Matrix Metalloproteinases; D001205:Ascorbic Acid; D019807:Iodoacetic Acid
keywords: chondrocyte; interleukin; matrix metalloproteinase; osteoarthritis; vitamin C