title: Characterization of RNA editome in primary and metastatic lung adenocarcinomas.
abstract: RNA editing results in post-transcriptional modification and could potentially contribute to carcinogenesis. However, RNA editing in advanced lung adenocarcinomas has not yet been studied. Based on whole genome and transcriptome sequencing data, we identified 1,071,296 RNA editing events from matched normal, primary and metastatic samples contributed by 24 lung adenocarcinoma patients, with 91.3% A-to-G editing on average, and found significantly more RNA editing sites in tumors than in normal samples. To investigate cancer relevant editing events, we detected 67,851 hyper-editing sites in primary and 50,480 hyper-editing sites in metastatic samples. 46 genes with hyper-editing in coding regions were found to result in amino acid alterations, while hundreds of hyper-editing events in non-coding regions could modulate splicing or gene expression, including genes related to tumor stage or clinic prognosis. Comparing RNA editome of primary and metastatic samples, we also discovered hyper-edited genes that may promote metastasis development. These findings showed a landscape of RNA editing in matched normal, primary and metastatic tissues of lung adenocarcinomas for the first time and provided new insights to understand the molecular characterization of this disease.
authors.affiliation: BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China.|BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Peng
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.|Department of Electrical and Computer Engineering, Donnelly Centre for Cellular and Biomolecular Research, University of Toronto, Toronto, Ontario M5S 3G4, Canada.
authors.forename: Leo J
authors.initials: LJ
authors.lastname: Lee
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Xiong
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Su
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Junhua
authors.initials: J
authors.lastname: Rao
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.|Guangzhou Institute of Respiratory Disease & State Key Laboratory of Respiratory Disease, Guangzhou 510120, China.|Research Center for Translational Medicine, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.
authors.forename: Dakai
authors.initials: D
authors.lastname: Xiao
authors.affiliation: Department of Thoracic Surgery, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 510120, China.|Guangzhou Institute of Respiratory Disease & State Key Laboratory of Respiratory Disease, Guangzhou 510120, China.|National Clinical Research Center for Respiratory Disease, Guangzhou 510120, China.
authors.forename: Jianxing
authors.initials: J
authors.lastname: He
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.|Department of Biology, University of Copenhagen, Copenhagen N DK-2200, Denmark.
authors.forename: Kui
authors.initials: K
authors.lastname: Wu
authors.affiliation: BGI-Shenzhen, Shenzhen 518083, China.
authors.forename: Dongbing
authors.initials: D
authors.lastname: Liu
mesh_terms: D000230:Adenocarcinoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D000077192:Adenocarcinoma of Lung; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling / Q000379:methods; D006801:Humans; D053208:Kaplan-Meier Estimate; D008175:Lung Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology*; D017393:RNA Editing / Q000235:genetics*; D017423:Sequence Analysis, RNA / Q000379:methods; D059467:Transcriptome
keywords: RNA editing; hyper-editing; lung adenocarcinoma; metastatic; primary