title: MicroRNA-497 suppresses cell proliferation and induces apoptosis through targeting PBX3 in human multiple myeloma.
abstract: Aberrant expression of microRNA-497 (miRN-497) is implicated in development and progression of multiple types of cancers. However, the biological function and underlying mechanism of miR-497 in multiple myeloma (MM) remains unclear. Thus, we studied the potential biological roles of miR-497 in MM. The expression of miR-497 was examined in multiple myeloma and normal plasma cells by qRT-PCR. Biological functions of miR-497 were analyzed using cell proliferation, colony formation, cell cycle, apoptosis and luciferase assaysin vitro, as well as via tumorigenicityin vivoanalysis. Here, we observed reduced expression of miR-497 in MM plasma samples and cell lines. Ectopic expression of miR-497 dramatically suppressed cell proliferation and clonogenicity, as well as induced cell arrest at G0/G1 stage and apoptosisin vitro. Mechanistic investigation assays showed that Pre-B-cellleukemia transcription factor 3 (PBX3) was a novel and direct downstream target of miR-497. Interestingly, overexpression of PBX3 partially reverted the effect of miR-497 in MM cells. In xenograft model, overexpression of miR-497 inhibited tumorigenicity by repressing PBX3. These findings collectively suggested that miR-497 functioned as tumor suppressor in MM by directly targeting PBX3, supporting its utility as a novel and potential therapeutic agent for MM therapy.
authors.affiliation: Department of Blood Transfusion, China-Japan Union Hospital of Jilin University 126 Xiantai Street, Changchun 130033, China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Yu
authors.affiliation: Shihezi University 221 North Fourth Road, Shihezi 832003, China.
authors.forename: Xuanhe
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Pathology, China-Japan Union Hospital of Jilin University 126 Xiantai Street, Changchun 130033, China.
authors.forename: Lirong
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Blood Transfusion, China-Japan Union Hospital of Jilin University 126 Xiantai Street, Changchun 130033, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Blood Transfusion, China-Japan Union Hospital of Jilin University 126 Xiantai Street, Changchun 130033, China.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Pan
authors.affiliation: Department of The 3rd Neurology, China-Japan Union Hospital of Jilin University 126 Xiantai Street, Changchun 130033, China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Department of Clinical Laboratory, The First Hospital of Jilin University 71 Xinmin Street, Changchun 130021, China.
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Pang
keywords: PBX3; apoptosis; miR-497; multiple myeloma; proliferation