title: Preparation of Polymer@AuNPs with Droplets Approach for Sensing Serum Copper Ions.
abstract: A microfluidic droplet synthesis approach for the preparation of poly N-isopropylacrylamide protected gold nanoparticles (PNIPAm@AuNPs) was presented here. Well-dispersed PNIPAm@AuNPs could be generated within 8 min. On the basis of the aggregation-induced UV-vis adsorption intensity increasing mechanism, the PNIPAm@AuNPs-based colorimetric probe displayed high sensitivity and good selectivity for sensing copper ions. A linear calibration of relative UV-vis adsorption intensity increasing versus copper ions concentration was obtained within 5.0-750.0 μM, and the limit of detection was 2.5 μM. Furthermore, after copper ions were injected in rat, a metabolic assay was developed with the proposed probe. The results indicated that the droplet microfluidic synthesis system could provide a new way for preparation of polymer@AuNPs with good polydispersity index and showed great potential of polymer@AuNPs-based sensing probe for application in biological and clinical analysis.
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Lab of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.|University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Juan
authors.initials: J
authors.lastname: Qiao
authors.affiliation: Beijing Institute of Petro-chemical Technology , Beijing 102617, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Ding
authors.affiliation: Xi'an University of Science and Technology , Shaanxi 710054, People's Republic of China.
authors.forename: Qianrong
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Beijing Institute of Petro-chemical Technology , Beijing 102617, People's Republic of China.
authors.forename: Rongyue
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Beijing National Laboratory for Molecular Sciences; Key Lab of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences , Beijing 100190, People's Republic of China.|University of Chinese Academy of Sciences , Beijing 100049, People's Republic of China.
authors.forename: Li
authors.identifier: 0000-0001-8549-7287
authors.initials: L
authors.lastname: Qi
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D015374:Biosensing Techniques; D002412:Cations / Q000097:blood; D003124:Colorimetry / Q000379:methods; D003300:Copper / Q000097:blood*; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D008297:Male; D053768:Metal Nanoparticles / Q000737:chemistry*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation*; D017207:Rats, Sprague-Dawley; D013056:Spectrophotometry, Ultraviolet / Q000379:methods
chemical_list: D000180:Acrylic Resins; D002412:Cations; C052970:poly-N-isopropylacrylamide; D006046:Gold; D003300:Copper