title: Prostanoids counterbalance the synergism between endothelin-1 and angiotensin II in mesenteric veins of trained rats.
abstract: Exercise-induced adaptations of the modulating mechanisms that influence the angiotensin (Ang II) responses assume different features depending on the venous bed. In femoral veins, exercise mobilizes vasodilator prostanoids to cooperate with NO in order to maintain reduced Ang II responses. On the other hand, exercise's influence on the Ang II responses in veins that drain blood from the mesenteric region has been poorly described. Therefore, the present study aimed to identify the effects of a single bout of exercise, as well as exercise training, on the Ang II responses in mesenteric veins. The present study also aimed to investigate the involvement of prostanoids, NO and ET-1 in eventual exercise-induced modifications in these veins. To this end, mesenteric veins taken from resting-sedentary, exercised-sedentary, resting-trained and exercised-trained animals were studied in organ baths. In addition, the mRNA expression of prepro-endothelin-1 (ppET-1), as well as that of the ETAand ETBreceptors, were quantified by real-time PCR in these veins. The results show that, either in absence or in presence of L-NAME, the Ang II responses were not different between groups. In the presence of indomethacin, higher Ang II responses were observed in the resting-trained animals than in the resting-sedentary animals. This difference, however, disappeared when L-NAME, BQ-123 or BQ-788 were added during incubation. In addition, no differences in ppET-1, ETAor ETBmRNA expression were observed between groups. Furthermore, in the presence of PD123,319, the Ang II responses in the exercised-sedentary animals were higher than those in the resting-sedentary animals. In conclusion, exercise training mobilizes endothelin-1 (ET-1) to reinforce the Ang II-induced responses mainly through ETAactivation. On the other hand, vasodilator prostanoids are mobilized to act in parallel with NO in order to counterbalance the Ang II responses that have been potentiated by ET-1 in these trained animals.
authors.affiliation: Laboratory of Pharmacology, Marília Medical School, SP, Brazil. Electronic address: agnaldochies@hotmail.com.
authors.forename: Agnaldo Bruno
authors.initials: AB
authors.lastname: Chies
authors.affiliation: Laboratory of Pharmacology, Marília Medical School, SP, Brazil.
authors.forename: Priscilla Bianca
authors.initials: PB
authors.lastname: de Oliveira
authors.affiliation: Department of Physiotherapy and Occupational Therapy, São Paulo State University (UNESP), Marília, SP, Brazil.
authors.forename: Patrícia de Souza
authors.initials: PS
authors.lastname: Rossignoli
authors.affiliation: Faculty Esefap of Tupã, SP, Brazil; Faculty of Alta Paulista (FAP), Tupã, SP, Brazil.
authors.forename: Rafaela de Fátima Ferreira
authors.initials: RFF
authors.lastname: Baptista
authors.affiliation: Laboratory of Genetic, Marília Medical School, São Paulo, Brazil.
authors.forename: Roger William
authors.initials: RW
authors.lastname: de Lábio
authors.affiliation: Laboratory of Genetic, Marília Medical School, São Paulo, Brazil.
authors.forename: Spencer Luiz Marques
authors.initials: SLM
authors.lastname: Payão
mesh_terms: D000804:Angiotensin II / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D019332:Endothelin-1 / Q000235:genetics* / Q000378:metabolism; D005268:Femoral Vein / Q000187:drug effects / Q000378:metabolism; D005786:Gene Expression Regulation; D007093:Imidazoles / Q000008:administration & dosage; D007213:Indomethacin / Q000008:administration & dosage; D008642:Mesenteric Veins / Q000187:drug effects / Q000378:metabolism*; D019331:NG-Nitroarginine Methyl Ester / Q000008:administration & dosage; D009569:Nitric Oxide / Q000378:metabolism; D009924:Organ Culture Techniques; D010805:Physical Conditioning, Animal; D011453:Prostaglandins / Q000008:administration & dosage; D011725:Pyridines / Q000008:administration & dosage; D012333:RNA, Messenger / Q000235:genetics; D051381:Rats; D044022:Receptor, Endothelin A / Q000235:genetics*; D044023:Receptor, Endothelin B / Q000235:genetics*; D014661:Vasoconstriction / Q000187:drug effects
chemical_list: D019332:Endothelin-1; D007093:Imidazoles; D011453:Prostaglandins; D011725:Pyridines; D012333:RNA, Messenger; D044022:Receptor, Endothelin A; D044023:Receptor, Endothelin B; C000622698:ednrb protein, rat; D000804:Angiotensin II; C073402:PD 123319; D009569:Nitric Oxide; D019331:NG-Nitroarginine Methyl Ester; D007213:Indomethacin
keywords: Angiotensin II; Endothelin-1; Exercise; Nitric oxide; Prostanoids; Vein