title: Xanthohumol, a Prenylated Chalcone from Hops, Inhibits the Viability and Stemness of Doxorubicin-Resistant MCF-7/ADR Cells.
abstract: Xanthohumol is a unique prenylated flavonoid in hops (Humulus lupulusL.) and beer. Xanthohumol has been shown to possess a variety of pharmacological activities. There is little research on its effect on doxorubicin-resistant breast cancer cells (MCF-7/ADR) and the cancer stem-like cells exiting in this cell line. In the present study, we investigate the effect of xanthohumol on the viability and stemness of MCF-7/ADR cells. Xanthohumol inhibits viability, induces apoptosis, and arrests the cell cycle of MCF-7/ADR cells in a dose-dependent manner; in addition, xanthohumol sensitizes the inhibition effect of doxorubicin on MCF-7/ADR cells. Interestingly, we also find that xanthohumol can reduce the stemness of MCF-7/ADR cells evidenced by the xanthohumol-induced decrease in the colony formation, the migration, the percentage of side population cells, the sphere formation, and the down-regulation of stemness-related biomarkers. These results demonstrate that xanthohumol is a promising compound targeting the doxorubicin resistant breast cancer cells and regulating their stemness, which, therefore, will be applied as a potential candidate for the development of a doxorubicin-resistant breast cancer agent and combination therapy of breast cancer.
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China. lmouc@hotmail.com.
authors.forename: Ming
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer (In Preparation), Qingdao 266061, China. yinhua@tsingtao.com.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Yin
authors.affiliation: College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. azrsqxk97@163.com.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer (In Preparation), Qingdao 266061, China. 15133634098@163.com.
authors.forename: Jianjun
authors.initials: J
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer (In Preparation), Qingdao 266061, China. qianzh@tsingtao.com.cn.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Qian
authors.affiliation: State Key Laboratory of Biological Fermentation Engineering of Beer (In Preparation), Qingdao 266061, China. miaojinlai@163.com.|Key Laboratory of Marine Bioactive Substance, the First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China. miaojinlai@163.com.
authors.forename: Jinlai
authors.initials: J
authors.lastname: Miao
mesh_terms: D000903:Antibiotics, Antineoplastic / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D014408:Biomarkers, Tumor / Q000378:metabolism; D001943:Breast Neoplasms / Q000188:drug therapy*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002599:Chalcone / Q000494:pharmacology; D004317:Doxorubicin / Q000494:pharmacology*; D019008:Drug Resistance, Neoplasm / Q000187:drug effects*; D005419:Flavonoids / Q000494:pharmacology*; D006801:Humans; D027582:Humulus / Q000737:chemistry; D061986:MCF-7 Cells; D014411:Neoplastic Stem Cells / Q000187:drug effects* / Q000473:pathology; D011427:Propiophenones / Q000494:pharmacology*; D018874:Spheroids, Cellular / Q000187:drug effects; D014407:Tumor Cells, Cultured
chemical_list: D000903:Antibiotics, Antineoplastic; D014408:Biomarkers, Tumor; D005419:Flavonoids; D011427:Propiophenones; D002599:Chalcone; D004317:Doxorubicin; C104536:xanthohumol
keywords: doxorubicin resistance; stemness; viability; xanthohumol