title: Sequential delivery of chlorhexidine acetate and bFGF from PLGA-glycol chitosan core-shell microspheres.
abstract: Wound treatment should meet the challenge both of preventing infection and promoting wound healing. To design a sequential delivery system for wound healing, PLGA-glycol chitosan (GC) core-shell microspheres containing chlorhexidine acetate (CHA) at the GC shell and bFGF in the core of PLGA microspheres were fabricated using emulsion-solvent evaporation method. SEM showed that the microspheres were all spherical in shape with a smooth surface. The average size of PLGA-GC microspheres increased due to the GC coating on the surface. The results of release profiles and fluorescence images indicated that PLGA-GC microspheres had an ability to deliver drugs in sequence. The CHA was rapidly released, whereas the proteins presented a sustained release. The release behavior could be modulated by changing the GC amount. Antibacterial assay and cell proliferation tests suggested that the released CHA and bFGF retained their antimicrobial activity and bioactivity during preparation. The microspheres exhibited non-cytotoxicity against 3T3 cells and had a good biocompatibility. These results demonstrated that PLGA-GC core-shell microspheres could be a promising controlled release system of delivering drugs and proteins in sequence for wound healing.
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Ming-Mao
authors.initials: MM
authors.lastname: Chen
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Cao
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Liu
authors.affiliation: State Key Lab of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Fei-Fei
authors.initials: FF
authors.lastname: Song
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China.
authors.forename: Jing-Di
authors.initials: JD
authors.lastname: Chen
authors.affiliation: Institute of Biomedical and Pharmaceutical Technology, Fuzhou University, Fuzhou 350002, China; Key Laboratory of Biomedical Material of Tianjin, Institute of Biomedical Engineering, Chinese Academy of Medical Science & Peking Union Medical College, Tianjin 300192, China. Electronic address: zhangqiq@126.com.
authors.forename: Qi-Qing
authors.initials: QQ
authors.lastname: Zhang
authors.affiliation: College of Pharmacy & Key Laboratory of Pharmaceutical Quality Control of Hebei Province, Hebei University, Baoding 071002, China. Electronic address: wenzhi_yang@sina.com.
authors.forename: Wen-Zhi
authors.initials: WZ
authors.lastname: Yang
mesh_terms: D016475:3T3 Cells; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D048271:Chitosan / Q000737:chemistry*; D002710:Chlorhexidine / Q000008:administration & dosage* / Q000737:chemistry; D016503:Drug Delivery Systems; D016222:Fibroblast Growth Factor 2 / Q000008:administration & dosage* / Q000737:chemistry; D006018:Glycols / Q000737:chemistry*; D019344:Lactic Acid / Q000737:chemistry*; D051379:Mice; D008855:Microscopy, Electron, Scanning; D008863:Microspheres; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D014945:Wound Healing
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D006018:Glycols; D016222:Fibroblast Growth Factor 2; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D048271:Chitosan; D002710:Chlorhexidine
keywords: Chlorhexidine acetate; Controlled release; Core-shell microspheres; Glycol chitosan; Growth factor; PLGA