title: Assessment of municipal wastewaters at various stages of treatment process as potential growth media for Chlorella sorokiniana under different modes of cultivation.
abstract: Wastewater utilization for microalgal biomass production is potentially the most economical route for its fuel and feed applications. In this study, suitability of various wastewater streams within a domestic wastewater treatment plant was evaluated for microalgal cultivation. Pre-treatment methods were evaluated to minimize bacterial load. Biomass, cell physiology, nutrient removal efficiencies and biochemical constituents of Chlorella sorokiniana were investigated in influent (INF) and anaerobic tank centrate (AC) under mixotrophic (Mixo) and heterotrophic (Hetero) cultivation. Promising biomass (77.14mgL-1d-1), lipid (24.91mgL-1d-1), protein (22.36mgL-1d-1) and carbohydrate (20.10mgL-1d-1) productivities were observed in Mixo AC with efficient ammonium (94.29%) and phosphate (83.30%) removal. Supplementation of urea at a concentration of 1500mgL-1further enhanced biomass (162.50mgL-1d-1), lipid (24.91mgL-1d-1), protein (22.36mgL-1d-1) and carbohydrate (20.10mgL-1d-1) productivities in Mixo AC. Urea supplemented mixotrophic cultivation of microalgae in AC is developed as a biomass production strategy.
authors.affiliation: Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.
authors.forename: Prathana
authors.initials: P
authors.lastname: Ramsundar
authors.affiliation: Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Guldhe
authors.affiliation: Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.
authors.forename: Poonam
authors.initials: P
authors.lastname: Singh
authors.affiliation: Institute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa. Electronic address: faizalb@dut.ac.za.
authors.forename: Faizal
authors.initials: F
authors.lastname: Bux
mesh_terms: D064751:Ammonium Compounds / Q000378:metabolism; D000693:Anaerobiosis; D018533:Biomass; D002241:Carbohydrates / Q000096:biosynthesis; D002708:Chlorella / Q000254:growth & development* / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry*; D052836:Heterotrophic Processes; D008055:Lipids / Q000096:biosynthesis; D058086:Microalgae / Q000254:growth & development* / Q000378:metabolism; D014865:Waste Disposal, Fluid / Q000379:methods*; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology
chemical_list: D064751:Ammonium Compounds; D002241:Carbohydrates; D003470:Culture Media; D008055:Lipids; D062065:Wastewater
keywords: Biomass; Heterotrophic; Microalgae; Mixotrophic; Wastewater