title: In vitro anti-MRSA activity of Couroupita guianensis extract and its component Tryptanthrin.
abstract: Couroupita guianensis is known in Brazil as 'Abricó-de-Macaco' and it has some attributes such as: antihypertensive, analgesic and anti-inflammatory activities. This study evaluated the antimicrobial activity of ethanolic extract and fractions of C. guianensis flowers and isolation of bioactive component. These extracts and fractions were subjected to agar diffusion, MIC, TLC and bioautography to bacteria, filamentous fungi and yeasts. Among the fractions of EtOH extract, the DCM fraction was the most active, particularly against Methicillin-resistant Staphylococcus aureus (MRSA) with MIC of 156 μg/mL. The active compound in this fraction was identified as Tryptanthrin, which showed promising antibacterial activity for MRSA showing MIC of 62.5 μg/mL. Ultrastructural analysis of MRSA incubated in the presence of Tryptanthrin by transmission electron microscope showed significant alterations in the cellular structure. Cytotoxicity tests demonstrated that DCM fraction and Tryptanthrin showed low toxicity, which makes it a promising candidate for alternative therapies to control and combat diseases.
authors.affiliation: a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.
authors.forename: Danielle Cristina Machado
authors.initials: DCM
authors.lastname: Costa
authors.affiliation: a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.|b Chemistry Institute , Federal University of Rio de Janeiro (UFRJ), CT, Ilha do Fundão , Rio de Janeiro , Brazil.
authors.forename: Mariana Maria Barros de
authors.identifier: http://orcid.org/0000-0001-8997-1206
authors.initials: MMB
authors.lastname: Azevedo
authors.affiliation: a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.
authors.forename: Davi Oliveira E
authors.initials: DOE
authors.lastname: Silva
authors.affiliation: a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.
authors.forename: Maria Teresa Villela
authors.initials: MTV
authors.lastname: Romanos
authors.affiliation: a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.
authors.forename: Thais Cristina Baeta Soares
authors.initials: TCBS
authors.lastname: Souto-Padrón
authors.affiliation: a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.
authors.forename: Celuta Sales
authors.initials: CS
authors.lastname: Alviano
authors.affiliation: a Institute of Microbiology Paulo de Góes , Federal University of Rio de Janeiro (IMPG-UFRJ), CCS, Ilha do Fundão , Rio de Janeiro , Brazil.
authors.forename: Daniela Sales
authors.initials: DS
authors.lastname: Alviano
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects; D001938:Brazil; D002522:Chlorocebus aethiops; D035264:Flowers / Q000737:chemistry; D005658:Fungi / Q000187:drug effects; D031381:Lecythidaceae / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D046529:Microscopy, Electron, Transmission; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D010946:Plants, Medicinal / Q000737:chemistry; D011799:Quinazolines / Q000494:pharmacology* / Q000633:toxicity; D014709:Vero Cells
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D011799:Quinazolines; C046243:tryptanthrine
keywords: Couroupita guianensis; MRSA; Tryptanthrin; antimicrobial activity