title: Comparative Analysis of the Rats' Gut Microbiota Composition in Animals with Different Ginsenosides Metabolizing Activity.
abstract: Following oral intake of Panax ginseng, major ginsenosides are metabolized to deglycosylated ginsenosides by gut microbiota before absorption into the blood. As the composition of gut microbiota varies between individuals, metabolic activities are significantly different. We selected 6 rats with low efficiency metabolism (LEM) and 6 rats with high efficiency metabolism (HEM) from 60 rats following oral administration of Panax ginseng extract, and analyzed their gut microbiota composition using Illumina HiSeq sequencing of the 16S rRNA gene. The components of gut microbiota between the LEM and HEM groups were significantly different. Between the 2 groups, S24-7, Alcaligenaceae, and Erysipelotrichaceae occupied most OTUs of the HEM group, which was notably higher than the LEM group. Furthermore, we isolated Bifidobacterium animalis GM1 that could convert the ginsenoside Rb1 to Rd. The result implies that these specific intestinal bacteria may dominate the metabolism of Panax ginseng.
authors.affiliation: Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ( Yanbian University ), Ministry of Education, Park Road 977, Yanji City, Jilin Province 133002, China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Dong
authors.affiliation: Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ( Yanbian University ), Ministry of Education, Park Road 977, Yanji City, Jilin Province 133002, China.
authors.forename: Fang-Ling
authors.initials: FL
authors.lastname: Xuan
authors.affiliation: Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ( Yanbian University ), Ministry of Education, Park Road 977, Yanji City, Jilin Province 133002, China.
authors.forename: Fei-Liang
authors.initials: FL
authors.lastname: Zhong
authors.affiliation: Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ( Yanbian University ), Ministry of Education, Park Road 977, Yanji City, Jilin Province 133002, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ( Yanbian University ), Ministry of Education, Park Road 977, Yanji City, Jilin Province 133002, China.
authors.forename: Songquan
authors.identifier: http://orcid.org/0000-0002-7072-9011
authors.initials: S
authors.lastname: Wu
authors.affiliation: Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ( Yanbian University ), Ministry of Education, Park Road 977, Yanji City, Jilin Province 133002, China.
authors.forename: Donghao
authors.initials: D
authors.lastname: Li
authors.affiliation: Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular ( Yanbian University ), Ministry of Education, Park Road 977, Yanji City, Jilin Province 133002, China.
authors.forename: Lin-Hu
authors.identifier: http://orcid.org/0000-0002-7195-8078
authors.initials: LH
authors.lastname: Quan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D003106:Colon / Q000187:drug effects / Q000378:metabolism; D000069196:Gastrointestinal Microbiome / Q000187:drug effects / Q000235:genetics; D036145:Ginsenosides / Q000032:analysis / Q000378:metabolism / Q000493:pharmacokinetics*; D008658:Inactivation, Metabolic; D008297:Male; D005894:Panax; D010936:Plant Extracts / Q000008:administration & dosage / Q000493:pharmacokinetics; D012336:RNA, Ribosomal, 16S; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry
chemical_list: D036145:Ginsenosides; D010936:Plant Extracts; D012336:RNA, Ribosomal, 16S; C442759:ginsenoside Rb1
keywords: LC-MS/MS; Panax ginseng; ginsenoside; metabolism; rats gut microbiota