title: Antileukemic activity of an arsenomolybdate in the human HL-60 and U937 leukemia cells.
abstract: The antileukemic activity, mechanisms and serum albumin interactions of an arsenomolybdate, K2Na[AsMo6O21(O2CCH2NH3)3]·6H2O (1), was evaluated in the human leukemia HL-60 and U937 cells. The results indicated that 1 could inhibit the proliferation of both leukemia cell lines in a dose-dependent manner with the 50% lethal concentration (IC50) value of 8.61μM for HL-60 and 14.50μM for U937 at 24h, compare to the positive controls, all-trans retinoic acid (ATRA) with IC50value of 20.76μM and 14.85μM,and As2O3with IC50value of 6.40μM and 8.75μM at 24h, respectively (P<0.05). Furthermore, the anti-leukemia activity of compound 1 might be medicated by arresting the leukemic cells in the G1phase and inducing apoptosis via caspase-3 and bcl-2 regulatory proteins. Spectroscopic techniques results showed that the fluorescence of human serum albumin was quenched by compound 1, and the quenching mechanism was mainly static quenching. Compound 1 might be a potential medicinal candidate against acute promyelocytic leukemia.
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Hongqian
authors.initials: H
authors.lastname: Cao
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Jiaheng
authors.initials: J
authors.lastname: Sun
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Rui
authors.initials: R
authors.lastname: Tian
authors.affiliation: College of Basic Medical Science, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Dongbei
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China. Electronic address: qiyanfei@jlu.edu.cn.
authors.forename: Yanfei
authors.initials: Y
authors.lastname: Qi
authors.affiliation: College of Basic Medical Science, Jilin University, Changchun, Jilin 130021, PR China. Electronic address: ywei@jlu.edu.cn.
authors.forename: Wei
authors.initials: W
authors.lastname: Yang
authors.affiliation: School of Public Health, Jilin University, Changchun, Jilin 130021, PR China.
authors.forename: Juan
authors.initials: J
authors.lastname: Li
mesh_terms: D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001152:Arsenicals / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D015153:Blotting, Western; D053148:Caspase 3 / Q000187:drug effects*; D002453:Cell Cycle / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D004355:Drug Stability; D005434:Flow Cytometry; D005453:Fluorescence; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D015473:Leukemia, Promyelocytic, Acute / Q000188:drug therapy*; D008982:Molybdenum / Q000737:chemistry / Q000494:pharmacology*; D020298:U937 Cells
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D008982:Molybdenum; D053148:Caspase 3
keywords: Acute promyelocytic leukemia; Apoptosis; Arsenomolybdate; Polyoxometalates