title: Serum stearic acid/palmitic acid ratio as a potential predictor of diabetes remission after Roux-en-Y gastric bypass in obesity.
abstract: Endogenous fatty acid metabolism that results in elongation and desaturation lipid products is thought to play a role in the development of type 2 diabetes mellitus (T2DM). In this study, we evaluated the potential of estimated elongase and desaturase activities for use as predictive markers for T2DM remission after Roux-en-Y gastric bypass (RYGB). The results of a targeted metabolomics approach from 2 independent studies were used to calculate 24 serum FA concentration ratios (product/precursor). Gene expression data from an open public data set was also analyzed. In a longitudinal study of 38 obese diabetic patients with RYGB, we found higher baseline stearic acid/palmitic acid (S/P) ratio. This ratio reflects anelovl6-encoded elongase enzyme activity that has been found to be associated with greater possibility for diabetes remission after RYGB [odds ratio, 2.16 (95% CI 1.10-4.26)], after adjustment for age, gender, body mass index, diabetes duration, glycosylated hemoglobin A1c, and fasting C-peptide. Our results were validated by examination of postsurgicalelovl6gene expression in morbidly obese patients. The association of S/P with the metabolic status of obese individuals was further validated in a cross-sectional cohort of 381 participants. In summary, higher baseline S/P was associated with greater probability of diabetes remission after RYGB and may serve as a diagnostic marker in preoperative patient assessment. - Zhao, L., Ni, Y., Yu, H., Zhang, P., Zhao, A., Bao, Y., Liu, J., Chen, T., Xie, G., Panee, J., Chen, W., Rajani, C., Wei, R., Su, M., Jia, W., Jia, W. Serum stearic acid/palmitic acid ratio as a potential predictor of diabetes remission after Roux-en-Y gastric bypass in obesity.
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.|College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai, China.
authors.forename: Linjing
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.|University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ni
authors.affiliation: Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Haoyong
authors.initials: H
authors.lastname: Yu
authors.affiliation: Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; and.
authors.forename: Pin
authors.initials: P
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Aihua
authors.initials: A
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.|Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Yuqian
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Jiajian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Tianlu
authors.initials: T
authors.lastname: Chen
authors.affiliation: University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors.forename: Guoxiang
authors.initials: G
authors.lastname: Xie
authors.affiliation: Department of Cell and Molecular Biology, John A. Burns School of Medicine, University of Hawaii, Manoa, Hawaii, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Panee
authors.affiliation: University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors.forename: Wenlian
authors.initials: W
authors.lastname: Chen
authors.affiliation: University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors.forename: Cynthia
authors.initials: C
authors.lastname: Rajani
authors.affiliation: University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors.forename: Runmin
authors.initials: R
authors.lastname: Wei
authors.affiliation: University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors.forename: Mingming
authors.initials: M
authors.lastname: Su
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; wpjia@sjtu.edu.cn.|Department of Endocrinology and Metabolism, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China.
authors.forename: Weiping
authors.initials: W
authors.lastname: Jia
authors.affiliation: Shanghai Key Laboratory of Diabetes Mellitus and Center for Translational Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, China; wjia@cc.hawaii.edu.|University of Hawaii Cancer Center, Honolulu, Hawaii, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Jia
mesh_terms: D000123:Acetyltransferases / Q000235:genetics / Q000378:metabolism; D000328:Adult; D000368:Aged; D015415:Biomarkers / Q000097:blood; D003920:Diabetes Mellitus / Q000097:blood* / Q000453:epidemiology; D000081031:Fatty Acid Elongases; D005260:Female; D015390:Gastric Bypass; D006801:Humans; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000150:complications / Q000601:surgery*; D019308:Palmitic Acid / Q000097:blood*; D013229:Stearic Acids / Q000097:blood*
chemical_list: D015415:Biomarkers; C000630955:ELOVL6 protein, human; D013229:Stearic Acids; D019308:Palmitic Acid; C031183:stearic acid; D000123:Acetyltransferases; D000081031:Fatty Acid Elongases
keywords: UPLC/QTOFMS; bariatric surgery; elovl6; fatty acid metabolism; targeted metabolomics