title: Overexpression of teneurin transmembrane protein 1 is a potential marker of disease progression in papillary thyroid carcinoma.
abstract: Although papillary thyroid cancer is a relatively indolent malignancy, its progression may be associated with dedifferentiation and resistance to radioactive iodine treatment. In this study, patterns of differentially expressed genes in association with disease progression were systemically evaluated. We firstly performed transcriptome analyses for four matched cancerous and noncancerous tissue pairs of the classical subtype of papillary thyroid cancer. Among the upregulated and downregulated genes, the expression of 164 and 183 genes increased and decreased, respectively, from stage I to stage IV. Functional enrichment and pathway analysis showed that angiogenesis pathway was upregulated, whereas oxidation-reduction and metabolism of reactive oxygen species were downregulated. Teneurin transmembrane protein 1 (TENM1) expression was highly upregulated in cancerous tissues and negative in benign thyroid tissues. By immunohistochemistry, TENM1 expression in papillary thyroid cancer was associated with the classical subtype (p = 0.018), extrathyroidal invasion (p = 0.001), BRAF V600E mutation (p < 0.001), and an advanced stage (p = 0.019). Taken together, our results indicate that distinct pathways are involved in papillary thyroid cancer progression, and TENM1 is a potential marker of cancer progression.
authors.affiliation: Department of Surgery, MacKay Memorial Hospital, Mackay Medical College, 92, Chung-Shan North Road, Section 2, Taipei, 10449, Taiwan.|Department of Pharmacology, Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.
authors.forename: Shih-Ping
authors.initials: SP
authors.lastname: Cheng
authors.affiliation: Department of Surgery, MacKay Memorial Hospital, Mackay Medical College, 92, Chung-Shan North Road, Section 2, Taipei, 10449, Taiwan.|Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan.|Department of Pharmacology, Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.
authors.forename: Ming-Jen
authors.initials: MJ
authors.lastname: Chen
authors.affiliation: Division of Endocrinology and Metabolism, Department of Internal Medicine, MacKay Memorial Hospital, Mackay Medical College, Taipei, Taiwan.
authors.forename: Ming-Nan
authors.initials: MN
authors.lastname: Chien
authors.affiliation: Department of Medical Research, MacKay Memorial Hospital, Taipei, Taiwan.
authors.forename: Chi-Hsin
authors.initials: CH
authors.lastname: Lin
authors.affiliation: Department of Surgery, MacKay Memorial Hospital, Mackay Medical College, 92, Chung-Shan North Road, Section 2, Taipei, 10449, Taiwan.|Department of Pharmacology, Graduate Institute of Medical Sciences, Taipei Medical University, Taipei, Taiwan.
authors.forename: Jie-Jen
authors.initials: JJ
authors.lastname: Lee
authors.affiliation: Department of Surgery, MacKay Memorial Hospital, Mackay Medical College, 92, Chung-Shan North Road, Section 2, Taipei, 10449, Taiwan. surg.mmh@gmail.com.
authors.forename: Chien-Liang
authors.initials: CL
authors.lastname: Liu
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015415:Biomarkers / Q000032:analysis*; D002291:Carcinoma, Papillary / Q000175:diagnosis* / Q000473:pathology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009419:Nerve Tissue Proteins / Q000032:analysis*; D011379:Prognosis; D019063:Tenascin / Q000032:analysis*; D000077273:Thyroid Cancer, Papillary; D013964:Thyroid Neoplasms / Q000175:diagnosis* / Q000473:pathology*
chemical_list: D015415:Biomarkers; D009419:Nerve Tissue Proteins; D019063:Tenascin; C120530:teneurin-1
keywords: Disease progression; Next-generation sequencing; Papillary thyroid cancer; RNA-Seq; TENM1