title: Comparison of 3D-Printed Poly-ɛ-Caprolactone Scaffolds Functionalized with Tricalcium Phosphate, Hydroxyapatite, Bio-Oss, or Decellularized Bone Matrix<sup/>.
abstract: Three-dimensional (3D)-printing facilitates rapid, custom manufacturing of bone scaffolds with a wide range of material choices. Recent studies have demonstrated the potential for 3D-printing bioactive (i.e., osteo-inductive) scaffolds for use in bone regeneration applications. In this study, we 3D-printed porous poly-ɛ-caprolactone (PCL) scaffolds using a fused deposition modeling (FDM) process and functionalized them with mineral additives that have been widely used commercially and clinically: tricalcium phosphate (TCP), hydroxyapatite (HA), Bio-Oss (BO), or decellularized bone matrix (DCB). We assessed the "print quality" of the composite scaffolds and found that the print quality of PCL-TCP, PCL-BO, and PCL-DCB measured ∼0.7 and was statistically lower than PCL and PCL-HA scaffolds (∼0.8). We found that the incorporation of mineral particles did not significantly decrease the compressive modulus of the graft, which was on the order of 260 MPa for solid blocks and ranged from 32 to 83 MPa for porous scaffolds. Raman spectroscopy revealed the surfaces of the scaffolds maintained the chemical profile of their dopants following the printing process. We evaluated the osteo-inductive properties of each scaffold composite by culturing adipose-derived stromal/stem cells in vitro and assessing their differentiation into osteoblasts. The calcium content (normalized to DNA) increased significantly in PCL-TCP (p < 0.05), PCL-BO (p < 0.001), and PCL-DCB (p < 0.0001) groups relative to PCL only. The calcium content also increased in PCL-HA but was not statistically significant (p > 0.05). Collagen 1 expression was 10-fold greater than PCL in PCL-BO and PCL-DCB (p < 0.05) and osteocalcin expression was 10-fold greater in PCL-BO and PCL-DCB (p < 0.05) as measured by quantitative-real time-polymerase chain reaction. This study suggests that PCL-BO and PCL-DCB hybrid material may be advantageous for bone healing applications over PCL-HA or PCL-TCP blends.
authors.affiliation: 1 Translational Tissue Engineering Center, Johns Hopkins University School of Medicine , Baltimore, Maryland.|2 Department of Biomedical Engineering, Johns Hopkins University School of Medicine , Baltimore, Maryland.
authors.forename: Ethan
authors.initials: E
authors.lastname: Nyberg
authors.affiliation: 1 Translational Tissue Engineering Center, Johns Hopkins University School of Medicine , Baltimore, Maryland.|2 Department of Biomedical Engineering, Johns Hopkins University School of Medicine , Baltimore, Maryland.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rindone
authors.affiliation: 3 Department of Plastic and Reconstructive Surgery, Johns Hopkins University School of Medicine , Baltimore, Maryland.
authors.forename: Amir
authors.initials: A
authors.lastname: Dorafshar
authors.affiliation: 1 Translational Tissue Engineering Center, Johns Hopkins University School of Medicine , Baltimore, Maryland.|2 Department of Biomedical Engineering, Johns Hopkins University School of Medicine , Baltimore, Maryland.|4 Department of Materials Science and Engineering, Johns Hopkins University , Baltimore, Maryland.|5 Institute for NanoBioTechnology, Johns Hopkins University , Baltimore, Maryland.
authors.forename: Warren L
authors.initials: WL
authors.lastname: Grayson
mesh_terms: D001857:Bone Matrix / Q000737:chemistry*; D002130:Calcium Phosphates / Q000737:chemistry*; D002454:Cell Differentiation; D002478:Cells, Cultured; D017886:Durapatite / Q000737:chemistry*; D006801:Humans; D008903:Minerals / Q000737:chemistry*; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D011091:Polyesters / Q000737:chemistry*; D066330:Printing, Three-Dimensional; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: C077540:Bio-Oss; D002130:Calcium Phosphates; D008903:Minerals; D011091:Polyesters; C016240:polycaprolactone; D017886:Durapatite; C018392:tricalcium phosphate
keywords: BioOss; additive manufacturing; adipose-derived stem cells; bone tissue engineering; decellularized bone matrix; hydroxyapatite; tricalcium phosphate