title: Cell adhesive peptides functionalized on CoCr alloy stimulate endothelialization and prevent thrombogenesis and restenosis.
abstract: Immobilization of bioactive peptide sequences on CoCr surfaces is an effective route to improve endothelialization, which is of great interest for cardiovascular stents. In this work, we explored the effect of physical and covalent immoblization of RGDS, YIGSR and their equimolar combination peptides on endothelial cells (EC) and smooth muscle cell (SMC) adhesion and on thrombogenicity. We extensively investigated using RT-qPCR, the expression by ECs cultured on functionalised CoCr surfaces of different genes. Genes relevant for adhesion (ICAM-1 and VCAM-1), vascularization (VEGFA, VEGFR-1 and VEGFR-2) and anti-thrombogenicity (tPA and eNOS) were over-expressed in the ECs grown to covalently functionalized CoCr surfaces compared to physisorbed and control surfaces. Pro-thrombogenic genes expression (PAI-1 and vWF) decreased over time. Cell co-cultures of ECs/SMCs found that functionalization increased the amount of adhered ECs onto modified surfaces compared to plain CoCr, independently of the used peptide and the strategy of immobilization. SMCs adhered less compared to ECs in all surfaces. All studied peptides showed a lower platelet cell adhesion compared to TCPS. Covalent functionalization of CoCr surfaces with an equimolar combination of RGDS and YIGSR represented prevailing strategy to enhance the early stages of ECs adhesion and proliferation, while preventing SMCs and platelet adhesion. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 973-983, 2017.
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, ETSEIB, Technical University of Catalonia (UPC), Barcelona, 08028, Spain.|Centre for Research in NanoEngineering (CRNE), UPC, Barcelona, 08028, Spain.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Castellanos
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, ETSEIB, Technical University of Catalonia (UPC), Barcelona, 08028, Spain.|Centre for Research in NanoEngineering (CRNE), UPC, Barcelona, 08028, Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Guillem-Marti
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, ETSEIB, Technical University of Catalonia (UPC), Barcelona, 08028, Spain.|Centre for Research in NanoEngineering (CRNE), UPC, Barcelona, 08028, Spain.
authors.forename: Carlos
authors.initials: C
authors.lastname: Mas-Moruno
authors.affiliation: Hemotherapy-Hemostasis Department, Centre de Diagnòstic Biomèdic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clinic, Universitat de Barcelona, Barcelona, 08036, Spain.
authors.forename: Maribel
authors.initials: M
authors.lastname: Díaz-Ricart
authors.affiliation: Hemotherapy-Hemostasis Department, Centre de Diagnòstic Biomèdic, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clinic, Universitat de Barcelona, Barcelona, 08036, Spain.
authors.forename: Ginés
authors.initials: G
authors.lastname: Escolar
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, ETSEIB, Technical University of Catalonia (UPC), Barcelona, 08028, Spain.|Centre for Research in NanoEngineering (CRNE), UPC, Barcelona, 08028, Spain.|Institute for Bioengineering of Catalonia (IBEC), Barcelona, 08028, Spain.
authors.forename: Maria Pau
authors.initials: MP
authors.lastname: Ginebra
authors.affiliation: Universitat Internacional de Catalunya (UIC), Barcelona, 08017, Spain.
authors.forename: Francisco Javier
authors.initials: FJ
authors.lastname: Gil
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, ETSEIB, Technical University of Catalonia (UPC), Barcelona, 08028, Spain.|Centre for Research in NanoEngineering (CRNE), UPC, Barcelona, 08028, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Pegueroles
authors.affiliation: Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, ETSEIB, Technical University of Catalonia (UPC), Barcelona, 08028, Spain.|Centre for Research in NanoEngineering (CRNE), UPC, Barcelona, 08028, Spain.
authors.forename: Jose María
authors.initials: JM
authors.lastname: Manero
mesh_terms: D001792:Blood Platelets / Q000378:metabolism; D002858:Chromium Alloys / Q000737:chemistry*; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D006083:Graft Occlusion, Vascular / Q000378:metabolism / Q000517:prevention & control*; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism*; D006801:Humans; D009842:Oligopeptides / Q000737:chemistry*; D013927:Thrombosis / Q000378:metabolism / Q000517:prevention & control*
chemical_list: D002858:Chromium Alloys; D020099:Coated Materials, Biocompatible; D009842:Oligopeptides; C046617:arginyl-glycyl-aspartyl-serine
keywords: CoCr alloy; cell coculture; functionalization; gene expression; platelet adhesion