title: Chemiluminescence cloth-based glucose test sensors (CCGTSs): A new class of chemiluminescence glucose sensors.
abstract: Chemiluminescence (CL) has been widely applied in many fields, but it is rarely used in a very simple, economical but effective way. In this work, for the first time, the CL cloth-based glucose test sensors (CCGTSs) are developed as a new class of CL glucose sensors, with no need for complicated, expensive device fabrication and peripheral equipment. When integrated with desirable hydrophobic barrier in the flow channel and gravity/capillary flow induced by a difference in height between the loading zone and the detection zone, a single cloth-based device can perform the whole CL process involving two steps of enzyme reactions. The wax screen-printing approach is used to fabricate ultra-cheap CCGTSs, the glucose detection involves the enzymatic oxidation of glucose to gluconic acid and H2O2followed by the horseradish peroxidase (HRP)-catalyzed oxidation of luminol by H2O2, and the emitted CL signals are heightened with p-iodophenol (PIP) and imaged using an inexpensive, portable CCD camera. Under optimized conditions, glucose can be determined over the range of 0.1-100mM, with a detection limit of 0.0948mM and an analysis time of less than 5.5min. Finally, the applicability and validity of the CCGTSs are demonstrated for the measurement of glucose in clinical urine and serum samples. Thus, the proposed sensors could provide great promise in applications in many areas, and may facilitate the achievement of point-of-care testing.
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Huijie
authors.initials: H
authors.lastname: Li
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Cuiling
authors.initials: C
authors.lastname: Liu
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. Electronic address: zhangcs@scnu.edu.cn.
authors.forename: Chunsun
authors.initials: C
authors.lastname: Zhang
mesh_terms: D001786:Blood Glucose / Q000032:analysis*; D004867:Equipment Design; D005949:Glucose Oxidase / Q000737:chemistry; D006029:Glycosuria / Q000652:urine*; D006735:Horseradish Peroxidase / Q000737:chemistry; D006801:Humans; D006861:Hydrogen Peroxide / Q000737:chemistry; D056656:Lab-On-A-Chip Devices; D057230:Limit of Detection; D049408:Luminescent Agents / Q000737:chemistry; D008163:Luminescent Measurements / Q000295:instrumentation*; D008165:Luminol / Q000737:chemistry; D000067716:Point-of-Care Testing; D013784:Textiles / Q000032:analysis
chemical_list: D001786:Blood Glucose; D049408:Luminescent Agents; D008165:Luminol; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase
keywords: Chemiluminescence; Glucose detection; Gravity/capillary flow; Microfluidic cloth-based analytical devices; Wax screen-printing