title: Nanocellulose based asymmetric composite membrane for the multiple functions in cell encapsulation.
abstract: We describe the nanocomposite membrane for cell encapsulation using nanocelluose hydrogels. One of the surfaces of bacterial cellulose (BC) pellicles was coated with collagen to enhance cell adhesion and the opposite side of the BC pellicles was coated with alginate to protect transplanted cells from immune rejection by the reduced pore size of the composite membrane. The morphology of nanocomposite membrane was observed by scanning electron microscopy and the permeability of the membrane was estimated by the release test using different molecular weights of polymer solution. The nanocomposite membrane was permeable to small molecules but impermeable to large molecules such as IgG antibodies inferring the potential use in cell implantation. In addition, the BC-based nanocomposite membrane showed a superior mechanical property due to the incorporation of compared with alginate membranes. The cells attached efficiently to the surface of BC composite membranes with a high level of cell viability as well as bioactivity. Cells grown on the BC composite membrane kit released dopamine freely to the medium through the membrane, which showed that the BC composite membrane would be a promising cell encapsulation material in implantation.
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Republic of Korea.
authors.forename: Minsung
authors.initials: M
authors.lastname: Park
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Republic of Korea.
authors.forename: Sungchul
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Republic of Korea.
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Biosystems and Biomaterials Science and Engineering, Seoul National University, Republic of Korea; Research Institute of Agriculture and Life Sciences, Seoul National University, Republic of Korea; Center for Food and Bioconvergence, Seoul National University, Republic of Korea. Electronic address: jhyun@snu.ac.kr.
authors.forename: Jinho
authors.initials: J
authors.lastname: Hyun
mesh_terms: D000818:Animals; D002482:Cellulose / Q000737:chemistry*; D003094:Collagen / Q000737:chemistry*; D020100:Hydrogels; D008855:Microscopy, Electron, Scanning; D053761:Nanocomposites; D016716:PC12 Cells; D011135:Polysaccharides, Bacterial / Q000737:chemistry*; D051381:Rats
chemical_list: D020100:Hydrogels; D011135:Polysaccharides, Bacterial; D002482:Cellulose; D003094:Collagen
keywords: Bacterial cellulose; Cell encapsulation; Nanocomposite; Permeability