title: Detection of Fusobacterium nucleatum in two cases of empyema and lung abscess using paromomycin-vancomycin supplemented Brucella HK agar.
abstract: Fusobacterium nucleatum was found in patients with empyema or pulmonary abscess, using paromomycin-vancomycin Brucella HK agar. In vitro examination revealed that growth of the strains differed significantly in different media. Clinicians should be aware that suboptimal F. nucleatum cultivation methods may result in an underestimation of its frequency.
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; First Department of Internal Medicine, Hokkaido University Hospital, Hokkaido, Japan.
authors.forename: Kentaro
authors.initials: K
authors.lastname: Nagaoka
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. Electronic address: k-yanagi@net.nagasaki-u.ac.jp.
authors.forename: Katsunori
authors.initials: K
authors.lastname: Yanagihara
authors.affiliation: Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
authors.forename: Yoshitomo
authors.initials: Y
authors.lastname: Morinaga
authors.affiliation: Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan; Global COE Program, Nagasaki University, Nagasaki, Japan.
authors.forename: Shigeru
authors.initials: S
authors.lastname: Kohno
mesh_terms: D000362:Agar; D000900:Anti-Bacterial Agents; D001431:Bacteriological Techniques; D002002:Brucella; D003470:Culture Media; D004653:Empyema / Q000175:diagnosis / Q000382:microbiology*; D005674:Fusobacterium Infections / Q000175:diagnosis / Q000382:microbiology*; D016967:Fusobacterium nucleatum / Q000235:genetics / Q000254:growth & development / Q000302:isolation & purification*; D006801:Humans; D008169:Lung Abscess / Q000175:diagnosis / Q000382:microbiology*; D008297:Male; D008875:Middle Aged; D010303:Paromomycin; D017422:Sequence Analysis, DNA; D014640:Vancomycin
chemical_list: D000900:Anti-Bacterial Agents; D003470:Culture Media; D010303:Paromomycin; D014640:Vancomycin; D000362:Agar
keywords: Anaerobes; Empyema; Fusobacterium nucleatum; Lung abscess