title: Translocation of an Intracellular Protein via Peptide-Directed Ligation.
abstract: Ligand-directed reactions allow chemical transformations at very low reactant concentrations and can thus provide access to efficient approaches for the post-translational modification of proteins. The development of these proximity-induced reactions is hampered by the number of appropriate ligands and the lack of design principles. Addressing these limitations, we report a proximity-induced labeling system which applies a moderate affinity peptide ligand. The design process was structure-guided and supported by molecular dynamics simulations. We show that selective protein labeling can be performed inside living cells enabling the subcellular translocation of a protein via ligand-directed chemistry for the first time.
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany.|Technical University Dortmund , Department of Chemistry and Chemical Biology, Otto-Hahn-Str. 6, 44227 Dortmund, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Stiller
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany.|Technical University Dortmund , Department of Chemistry and Chemical Biology, Otto-Hahn-Str. 6, 44227 Dortmund, Germany.
authors.forename: Dennis M
authors.initials: DM
authors.lastname: Krüger
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Brauckhoff
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany.
authors.forename: Marcel
authors.identifier: 0000-0001-9618-1376
authors.initials: M
authors.lastname: Schmidt
authors.affiliation: Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Janning
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany.
authors.forename: Hazem
authors.initials: H
authors.lastname: Salamon
authors.affiliation: Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany.|Technical University Dortmund , Department of Chemistry and Chemical Biology, Otto-Hahn-Str. 6, 44227 Dortmund, Germany.|Max Planck Institute of Molecular Physiology, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.|VU University Amsterdam , Department of Chemistry and Pharmaceutical Sciences, De Boelelaan 1108, 1081 HZ, Amsterdam, The Netherlands.
authors.forename: Tom N
authors.identifier: 0000-0003-0179-4116
authors.initials: TN
authors.lastname: Grossmann
mesh_terms: D006367:HeLa Cells; D006801:Humans; D008024:Ligands; D056004:Molecular Dynamics Simulation; D010455:Peptides / Q000737:chemistry*; D021381:Protein Transport; D011506:Proteins / Q000737:chemistry*
chemical_list: D008024:Ligands; D010455:Peptides; D011506:Proteins