title: Preparation and Identification of Monoclonal Antibody Against C1q/TNF-Related Protein 4.
abstract: CTRP4 (C1q/TNF-related protein 4) is a secreted cytokine homologous to adiponectin, which plays an important role in immunity and metabolism. This study was designed to generate CTRP4 monoclonal antibodies (MAbs). Splenocytes extracted from mice immunized with prokaryotic protein were fused with myeloma cells Sp2/0 to generate hybridoma cells. Three hybridoma strains (16, 33, and 35) were chosen and their MAbs were purified. The specificity and affinity were identified by Western blot and enzyme-linked immunosorbent assay, whereas CTRP4 endogenous expression was identified by immunofluorescence staining. These MAbs could be useful tools for basic research and potential clinical application.
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center , Beijing, China .
authors.forename: He
authors.initials: H
authors.lastname: Huang
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center , Beijing, China .
authors.forename: Xiaotong
authors.initials: X
authors.lastname: Wu
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center , Beijing, China .
authors.forename: Lulu
authors.initials: L
authors.lastname: Cao
authors.affiliation: Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center , Beijing, China .
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000737:chemistry / Q000302:isolation & purification*; D000918:Antibody Specificity; D015153:Blotting, Western; D003001:Cloning, Molecular; D016207:Cytokines / Q000008:administration & dosage / Q000032:analysis* / Q000235:genetics / Q000276:immunology; D004797:Enzyme-Linked Immunosorbent Assay / Q000379:methods*; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D005260:Female; D015870:Gene Expression; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D006825:Hybridomas / Q000276:immunology; D007114:Immunization; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D012680:Sensitivity and Specificity; D013154:Spleen / Q000166:cytology / Q000187:drug effects / Q000276:immunology
chemical_list: D000911:Antibodies, Monoclonal; C560750:C1QTNF4 protein, human; D016207:Cytokines
keywords: CTRP4; affinity; subcellular location