title: Identification of Stevioside Using Tissue Culture-Derived Stevia (Stevia rebaudiana) Leaves.
abstract: Stevioside is a natural sweetener from Stevia leaf, which is 300 times sweeter than sugar. It helps to reduce blood sugar levels dramatically and thus can be of benefit to diabetic people. Tissue culture is a very potential modern technology that can be used in large-scale disease-free stevia production throughout the year. We successfully produced stevia plant through in vitro culture for identification of stevioside in this experiment. The present study describes a potential method for identification of stevioside from tissue culture-derived stevia leaf. Stevioside in the sample was identified using HPLC by measuring the retention time. The percentage of stevioside content in the leaf samples was found to be 9.6%. This identification method can be used for commercial production and industrialization of stevia through in vitro culture across the world.
authors.affiliation: Laboratory of Food Preventive Medicine, Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan.
authors.forename: Md Ziaul
authors.initials: MZ
authors.lastname: Karim
authors.affiliation: Laboratory of Food Preventive Medicine, Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Uesugi
authors.affiliation: Laboratory of Food Preventive Medicine, Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan.
authors.forename: Noriyuki
authors.initials: N
authors.lastname: Nakayama
authors.affiliation: Professor and Director, Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh.
authors.forename: M Monzur
authors.initials: MM
authors.lastname: Hossain
authors.affiliation: Laboratory of Food Preventive Medicine, Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan.
authors.forename: Kohji
authors.initials: K
authors.lastname: Ishihara
authors.affiliation: Laboratory of Food Preventive Medicine, Department of Life Science, Faculty of Science, Okayama University of Science, Okayama, Japan.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Hamada
keywords: HPLC; in vitro; stevioside; sweetener; tissue culture