title: Genetic variations of 21 STR markers on chromosomes 13, 18, 21, X, and Y in the south Iranian population.
abstract: Quantitative fluorescent polymerase chain reaction (QF-PCR), in recent years, has been accepted as a rapid, high throughput, and sensitive method for prenatal diagnosis of common chromosomal aneuploidies. Since short tandem repeats (STRs) are the cornerstone of QF-PCR technique, selection of the most polymorphic STR markers is an essential step for a successful QF-PCR assay. The genetic variation parameters of each STR marker differ among different populations. In this study, we investigated the size, frequency, heterozygosity, polymorphism information content, power of discrimination, and other genetic polymorphism data for 21 STR markers on chromosomes 13, 18, 21, X, and Y in 1000 amniotic fluid samples obtained from south Iranian women. Our results showed that all the 21 STR markers are highly polymorphic and informative in our population. The heterozygosity, polymorphism information content, and power of discrimination of the markers were 62-91.1%, 0.61-0.91, and 0.830-0.976, respectively. The locus D18S386 was the most polymorphic STR, while the locus DXYS218 was the least polymorphic STR among all the studied STRs. The present study has provided extensive data regarding the efficiency of the 21 STR markers for diagnosis of chromosomes 13, 18, 21, X, and Y aneuploidies in the south Iranian population.
authors.affiliation: Medical Biotechnology Department, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: J
authors.initials: J
authors.lastname: Saberzadeh
authors.affiliation: Medical Biotechnology Department, School of Advanced Medical Sciences and Technology, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: M R
authors.initials: MR
authors.lastname: Miri
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.|Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.|Comprehensive Medical Genetic Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: M B
authors.initials: MB
authors.lastname: Tabei
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran.|Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran.|Comprehensive Medical Genetic Center, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Dianatpour
authors.affiliation: Department of Medical Genetics, School of Medical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran mfardaei@sums.ac.ir.|Transgenic Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran mfardaei@sums.ac.ir.|Comprehensive Medical Genetic Center, Shiraz University of Medical Sciences, Shiraz, Iran mfardaei@sums.ac.ir.
authors.forename: M
authors.initials: M
authors.lastname: Fardaei
mesh_terms: D000653:Amniotic Fluid / Q000737:chemistry; D002877:Chromosomes, Human / Q000235:genetics*; D002882:Chromosomes, Human, Pair 13 / Q000235:genetics; D002887:Chromosomes, Human, Pair 18 / Q000235:genetics; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics; D041321:Chromosomes, Human, X / Q000235:genetics; D041322:Chromosomes, Human, Y / Q000235:genetics; D005260:Female; D005787:Gene Frequency; D014644:Genetic Variation; D006801:Humans; D007492:Iran; D008297:Male; D018895:Microsatellite Repeats; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D044465:White People / Q000235:genetics*