title: Biodiversity of Trichoderma Community in the Tidal Flats and Wetland of Southeastern China.
abstract: To investigate the biodiversity of Trichoderma (Hypocreaceae) and their relation to sediment physical and chemical properties, we collected a total of 491 sediment samples from coastal wetlands (tidal flat and wetland) in Southeast China. Further, we applied two types of molecular approaches such as culture dependent and independent methods for identification of Trichoderma spp. A total of 254 isolates were obtained and identified to 13 species such as T. aureoviride, T. asperellum, T. harzianum, T. atroviride, T. koningiopsis, T. longibrachiatum, T. koningii. T. tawa, T. viridescens, T. virens, T. hamatum, T. viride, and T. velutinum by the culture-dependent (CD) method of these, T. tawa was newly described in China. Subsequently, the culture indepented method of 454 pyrosequencing analysis revealed a total of six species such as T. citrinoviride, T. virens, T. polysporum, T. harzianum/Hypocrea lixii and two unknown species. Notably, T. citrinoviride and T. polysporum were not found by the CD method. Therefore, this work revealed that the combination of these two methods could show the higher biodiversity of Trichoderma spp., than either of this method alone. Among the sampling sites, Hangzhou, Zhejiang Province, exhibited rich biodiversity and low in Fengxian. Correlation and Redundancy discriminant analysis (RDA) revealed that sediment properties of temperature, redox potential (Eh) and pH significantly influenced the biodiversity of Trichoderma spp.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P.R. China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, P.R. China.
authors.forename: Kandasamy
authors.initials: K
authors.lastname: Saravanakumar
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P.R. China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, P.R. China.
authors.forename: Chuanjin
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P.R. China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, P.R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Dou
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P.R. China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, P.R. China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P.R. China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, P.R. China.
authors.forename: Yaqian
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, P.R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai, P.R. China.|Key Laboratory of Urban Agriculture (South), Ministry of Agriculture, Shanghai, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D044822:Biodiversity; D002681:China; D004271:DNA, Fungal / Q000737:chemistry / Q000378:metabolism; D016002:Discriminant Analysis; D019015:Geologic Sediments / Q000382:microbiology; D010802:Phylogeny; D012621:Seasons; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D014242:Trichoderma / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D053833:Wetlands
chemical_list: D004271:DNA, Fungal