title: Liposomal Spherical Nucleic Acids for Regulating Long Noncoding RNAs in the Nucleus.
abstract: Emerging evidence indicates that long noncoding RNAs (lncRNAs) are actively involved in a number of developmental and tumorigenic processes. Here, the authors describe the first successful use of spherical nucleic acids as an effective nanoparticle platform for regulating lncRNAs in cells; specifically, for the targeted knockdown of the nuclear-retained metastasis associated lung adenocarcinoma transcript 1 (Malat1), a key oncogenic lncRNA involved in metastasis of several cancers. Utilizing the liposomal spherical nucleic acid (LSNA) constructs, the authors first explored the delivery of antisense oligonucleotides to the nucleus. A dose-dependent inhibition of Malat1 upon LSNA treatment as well as the consequent up-regulation of tumor suppressor messenger RNA associated with Malat1 knockdown are shown. These findings reveal the biologic and therapeutic potential of a LSNA-based antisense strategy in targeting disease-associated, nuclear-retained lncRNAs.
authors.affiliation: Department of Biomedical Engineering, International Institute for Nanotechnology, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Sprangers
authors.affiliation: Interdisciplinary Biological Sciences, International Institute for Nanotechnology, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Liangliang
authors.initials: L
authors.lastname: Hao
authors.affiliation: Department of Chemical and Biological Engineering, International Institute for Nanotechnology, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Resham J
authors.initials: RJ
authors.lastname: Banga
authors.affiliation: Department of Biomedical Engineering, International Institute for Nanotechnology, Northwestern University, Evanston, IL, 60208, USA.|Department of Chemical and Biological Engineering, International Institute for Nanotechnology, Northwestern University, Evanston, IL, 60208, USA.
authors.forename: Chad A
authors.initials: CA
authors.lastname: Mirkin
mesh_terms: D000072283:A549 Cells; D002467:Cell Nucleus; D006801:Humans; D008081:Liposomes / Q000378:metabolism*; D009696:Nucleic Acids / Q000378:metabolism*; D054735:Phosphorothioate Oligonucleotides / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism
chemical_list: D008081:Liposomes; C557041:MALAT1 long non-coding RNA, human; D009696:Nucleic Acids; D054735:Phosphorothioate Oligonucleotides; D062085:RNA, Long Noncoding
keywords: DNA nanoparticles; gene regulation; liposomal SNA; nuclear targeting; spherical nucleic acids