title: Temperature-responsive PLLA/PNIPAM nanofibers for switchable release.
abstract: Smart antimicrobial materials with on-demand drug release are highly desired for biomedical applications. Herein, we report about temperature-responsive poly(N-isopropylacrylamide) (PNIPAM) nanospheres doped with crystal violet (CV) and incorporated into the poly-l-lactide (PLLA) nanofibers. The nanofibers were prepared by electrospinning, using different initial polymers ratios. The morphology of the nanofibers and polymers distribution in the nanofibers were characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The interaction between PNIPAM and PLLA in the nanofibers was studied by Fourier transform infrared spectroscopy (FTIR) and its effect on the PNIPAM phase transition was also investigated. It was shown that by the changing of the environmental temperature across the lower critical solution temperature (LCST) of PNIPAM, the switchable wettability and controlled CV release can be achieved. The temperature-dependent release kinetics of CV from polymer nanofibers was investigated by ultraviolet-visible spectroscopy (UV-Vis). The temperature-responsive release of antibacterial CV was also tested for triggering of antibacterial activity, which was examined on Staphylococcus epidermidis (S. epidermidis) and Escherichia coli (E. coli). Thus, the proposed material is promising value for controllable drug-release.
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, Prague 166 28, Czech Republic.
authors.forename: Roman
authors.initials: R
authors.lastname: Elashnikov
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, Prague 166 28, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Slepička
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology, 16628 Prague, Czech Republic.
authors.forename: Silvie
authors.initials: S
authors.lastname: Rimpelova
authors.affiliation: Department of Biochemistry and Microbiology, University of Chemistry and Technology, 16628 Prague, Czech Republic.
authors.forename: Pavel
authors.initials: P
authors.lastname: Ulbrich
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, Prague 166 28, Czech Republic.
authors.forename: Vaclav
authors.initials: V
authors.lastname: Švorčík
authors.affiliation: Department of Solid State Engineering, University of Chemistry and Technology, Prague 166 28, Czech Republic. Electronic address: lyutakoo@vscht.cz.
authors.forename: Oleksiy
authors.initials: O
authors.lastname: Lyutakov
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000891:Anti-Infective Agents, Local / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry; D065546:Drug Liberation; D004926:Escherichia coli / Q000187:drug effects; D005840:Gentian Violet / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D057139:Nanofibers / Q000737:chemistry*; D044367:Phase Transition; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013212:Staphylococcus epidermidis / Q000187:drug effects; D013696:Temperature; D017155:Wettability
chemical_list: D000180:Acrylic Resins; D000891:Anti-Infective Agents, Local; D004337:Drug Carriers; D011091:Polyesters; C052970:poly-N-isopropylacrylamide; C033616:poly(lactide); D005840:Gentian Violet
keywords: Antibacterial; Electrospinning; Nanofibers; Polymer blends; Responsive release; Stimuli-responsive; Switchable wettability