title: Losartan Improves Palmitate-Induced Insulin Resistance in 3T3-L1 Adipocytes Through Upregulation of Src Phosphorylation.
abstract: Angiotensin II type 1 receptor blocker losartan has shown strongly anti-insulin resistance propertiesin vivoandin vitro; however, the underlying mechanisms are poorly understood. In this study, we demonstrate that losartan administration increased phosphorylation of Akt and its downstream Akt substrate of 160 kDa (AS160), enhanced plasma membrane translocation of glucose transporter type 4 (GLUT4), and increased glucose uptake, along with increased Src phosphorylation as well as reduced expression of docking protein 1(DOK1) in palmitate-treated 3T3-L1 adipocytes. The beneficial impacts of losartan on insulin signaling were diminished in Src-deficient 3T3-L1 adipocytes. In addition, suppressed expression of DOK1 by losartan was abolished by Src knockdown. Our results suggest that anti-insulin resistance ability of losartan is mediated by Src/DOK1/Akt pathway.
authors.affiliation: Department of Critical Care Medicine, The Second People's Hospital of Jingzhou, Jingzhou City 434000, China.
authors.forename: X
authors.initials: X
authors.lastname: Tian
authors.affiliation: Department of Endocrinology, The Central Hospital of Enshi Autonomous Prefecture, Enshi 445000, China.
authors.forename: M
authors.initials: M
authors.lastname: Ye
authors.affiliation: Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences, Wuhan 430071, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Pathology and Pathophysiology, Wuhan University School of Basic Medical Sciences, Wuhan 430071, China.
authors.forename: C
authors.initials: C
authors.lastname: Wang
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000378:metabolism* / Q000473:pathology; D000818:Animals; D007333:Insulin Resistance; D019808:Losartan / Q000494:pharmacology*; D051379:Mice; D019308:Palmitic Acid / Q000633:toxicity*; D010766:Phosphorylation / Q000187:drug effects; D015854:Up-Regulation / Q000187:drug effects*; D019061:src-Family Kinases / Q000378:metabolism*
chemical_list: D019308:Palmitic Acid; D019061:src-Family Kinases; D019808:Losartan