title: Decreased formation of branched-chain short fatty acids in Bacillus amyloliquefaciens by metabolic engineering.
abstract: OBJECTIVE
  To reduce the unpleasant odor during 1-deoxynojirimycin (DNJ) production, the genes of leucine dehydrogenase (bcd) and phosphate butryltransferase (ptb) were deleted from Bacillus amyloliquefaciens HZ-12, and the concentrations of branched-chain short fatty acids (BCFAs) and DNJ were compared.
  
  
  RESULTS
  By knockout of the ptb gene, 1.01 g BCFAs kg-1was produced from fermented soybean by HZ-12Δptb. This was a 56% decrease compared with that of HZ-12 (2.27 g BCFAs kg-1). Moreover, no significant difference was found in the DNJ concentration (0.7 g kg-1). After further deletion of the bcd gene from HZ-12Δptb, no BCFAs was detected in fermented soybeans with HZ-12ΔptbΔbcd, while the DNJ yield decreased by 26% compared with HZ-12.
  
  
  CONCLUSIONS
  HZ-12Δptb had decreased BCFAs formation but also maintained the stable DNJ yield, which contributed to producing DNJ-rich products with decreased unpleasant smell.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.|College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Mengjie
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China.
authors.forename: Shouwen
authors.initials: S
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, China. weixuetuan@mail.hzau.edu.cn.|College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. weixuetuan@mail.hzau.edu.cn.
authors.forename: Xuetuan
authors.identifier: http://orcid.org/0000-0001-5731-7824
authors.initials: X
authors.lastname: Wei
mesh_terms: D017485:1-Deoxynojirimycin / Q000378:metabolism*; D000069977:Bacillus amyloliquefaciens / Q000235:genetics / Q000378:metabolism*; D002849:Chromatography, Gas; D002851:Chromatography, High Pressure Liquid; D015536:Down-Regulation; D005227:Fatty Acids / Q000096:biosynthesis*; D005285:Fermentation; D005516:Food Microbiology; D015870:Gene Expression; D055786:Gene Knockout Techniques; D005798:Genes, Bacterial; D050803:Leucine Dehydrogenase / Q000378:metabolism; D060847:Metabolic Engineering; D009812:Odorants / Q000517:prevention & control; D010709:Phosphate Acetyltransferase / Q000378:metabolism; D013025:Glycine max / Q000378:metabolism
chemical_list: D005227:Fatty Acids; D017485:1-Deoxynojirimycin; D050803:Leucine Dehydrogenase; D010709:Phosphate Acetyltransferase
keywords: Bacillus amyloliquefaciens; Branched-chain short fatty acids; Leucine dehydrogenase; Metabolic engineering; Phosphotransbutyrylase