title: Serum Cytokine Signature That DiscriminatesHelicobacter pyloriPositive and Negative Juvenile Gastroduodenitis.
abstract: Gastroduodenitis caused byH. pylori, often acquired in early childhood, is found in about 50% of the adult population. AlthoughH. pyloriinfections can remain asymptomatic, its virulence factors usually trigger epithelial vacuolization and degeneration, loss of microvilli, disintegration of cytoplasm, and leukocyte accumulation. It is believed that leukocyte infiltration is driven by cytokines produced locally in infected tissue. However, so far little is known about changes in serum cytokines in juvenile patients infected withH. pylori. Serum cytokine profiles were analyzed in 62 juvenile patients diagnosed with gastroduodenitis using the Bio-Plex multiplex assay.H. pyloriinfection was confirmed in 32 patients, while 30 patients wereH. pylori-free. Cytokines CXCL5 and CXCL6, potent neutrophil chemoattractants, were upregulated in all patients diagnosed with gastroduodenitis. Serum levels of IL8, a prototype neutrophil attractant, remained unchanged in subjects with gastroduodenitis relative to controls. Therefore, our data suggest that CXCL5 and CXCL6 play a role in directing neutrophil trafficking into inflamed gastroduodenal tissue. In addition, the CCL25/GM-CSF ratio differed significantly betweenH. pylori-positive and -negative juveniles. Further, study is needed to evaluate the role of CCL25 and GM-CSF in the pathogenesis of the different etiologies of gastroduodenitis.
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University Kazan, Russia.
authors.forename: Svetlana F
authors.initials: SF
authors.lastname: Khaiboullina
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal UniversityKazan, Russia; Kazan State Medical UniversityKazan, Russia.
authors.forename: Sayar
authors.initials: S
authors.lastname: Abdulkhakov
authors.affiliation: Kazan State Medical University Kazan, Russia.
authors.forename: Alsu
authors.initials: A
authors.lastname: Khalikova
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University Kazan, Russia.
authors.forename: Dilyara
authors.initials: D
authors.lastname: Safina
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University Kazan, Russia.
authors.forename: Ekaterina V
authors.initials: EV
authors.lastname: Martynova
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University Kazan, Russia.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Davidyuk
authors.affiliation: Kazan State Medical University Kazan, Russia.
authors.forename: Felix
authors.initials: F
authors.lastname: Khuzin
authors.affiliation: Kazan State Medical University Kazan, Russia.
authors.forename: Rezeda
authors.initials: R
authors.lastname: Faizullina
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal UniversityKazan, Russia; Nevada Center for Biomedical ResearchReno, NV, USA.
authors.forename: Vincent C
authors.initials: VC
authors.lastname: Lombardi
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal UniversityKazan, Russia; Department of Clinical Laboratory Diagnostics, Kazan State Medical AcademyKazan, Russia.
authors.forename: Georgi V
authors.initials: GV
authors.lastname: Cherepnev
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University Kazan, Russia.
authors.forename: Albert A
authors.initials: AA
authors.lastname: Rizvanov
keywords: H. pylori; cytokines; gastroduodenitis; virulence factors