title: Stringent control of FLP recombinase in Escherichia coli.
abstract: Site specific recombinases are invaluable tools in molecular biology, and are emerging as powerful recorders of cellular events in synthetic biology. We have developed a stringently controlled FLP recombinase system in Escherichia coli using an arabinose inducible promoter combined with a weak ribosome binding site.
authors.affiliation: Biotechnology institute, 1500 Gortner Avenue, University of Minnesota, Saint Paul, MN 55108, United States. Electronic address: sbowden@umn.edu.
authors.forename: Steven D
authors.initials: SD
authors.lastname: Bowden
authors.affiliation: Department of Plant Biology, University of Minnesota, Saint Paul, MN 55108, United States.
authors.forename: Nagendra P
authors.initials: NP
authors.lastname: Palani
authors.affiliation: Biotechnology institute, 1500 Gortner Avenue, University of Minnesota, Saint Paul, MN 55108, United States; Department of Plant Biology, University of Minnesota, Saint Paul, MN 55108, United States. Electronic address: libourel@umn.edu.
authors.forename: Igor G L
authors.initials: IGL
authors.lastname: Libourel
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D004265:DNA Helicases / Q000235:genetics / Q000378:metabolism; D004254:DNA Nucleotidyltransferases / Q000235:genetics* / Q000378:metabolism; D004926:Escherichia coli / Q000201:enzymology* / Q000235:genetics*; D017853:Phosphotransferases (Alcohol Group Acceptor) / Q000235:genetics / Q000378:metabolism; D010957:Plasmids; D011401:Promoter Regions, Genetic; D011995:Recombination, Genetic; D015534:Trans-Activators / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D015534:Trans-Activators; C045264:replication initiator protein; D017853:Phosphotransferases (Alcohol Group Acceptor); C022550:ribulokinase; D004254:DNA Nucleotidyltransferases; C045119:FLP recombinase; C077570:Site-specific recombinase; D004265:DNA Helicases
keywords: Escherichia coli, arabinose; Flp recombinase; Gene expression; Repression