title: Tricholoma matsutakeAqueous Extract Induces Hepatocellular Carcinoma Cell Apoptosis via Caspase-Dependent Mitochondrial Pathway.
abstract: Tricholoma matsutake, one of widely accepted functional mushrooms, possesses various pharmacological activities, and its antitumor effect has become an important research point. Our study aims to evaluate the cytotoxicity activities ofT. matsutakeaqueous extract (TM) in HepG2 and SMMC-7721 cells. Inin vitroexperiments, TM strikingly reduced cell viability, promoted cell apoptosis, inhibited cell migration ability, induced excessive generation of ROS, and caused caspases cascade and mitochondrial membrane potential dissipation in hepatocellular carcinoma cells. Inin vivoexperiments, 14-day TM treatment strongly suppressed tumor growth in HepG2 and SMMC-7721-xenografted nude mice without influence on their body weights and liver function. Furthermore, TM increased the levels of cleaved poly-ADP-ribose polymerase (PARP), Bad, and Bax and reduced the expressions of B-cell lymphoma 2 (Bcl-2) in treated cells and tumor tissues. All aforementioned results suggest that caspase-dependent mitochondrial apoptotic pathways are involved in TM-mediated antihepatocellular carcinoma.
authors.affiliation: Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China; School of Life Sciences, Jilin University, Jilin 130012, China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China; Southern Research Institute, Jilin University, Zhuhai 519000, China.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Jilin University, Jilin 130012, China.
authors.forename: Xinrui
authors.initials: X
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Jilin University, Jilin 130012, China.
authors.forename: Guangsheng
authors.initials: G
authors.lastname: Cai
authors.affiliation: School of Life Sciences, Jilin University, Jilin 130012, China.
authors.forename: Shengshu
authors.initials: S
authors.lastname: An
authors.affiliation: School of Life Sciences, Jilin University, Jilin 130012, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China; School of Life Sciences, Jilin University, Jilin 130012, China.
authors.forename: Lirong
authors.identifier: 0000-0002-6098-1504
authors.initials: L
authors.lastname: Teng
authors.affiliation: Zhuhai College of Jilin University, Jilin University, Zhuhai 519000, China; School of Life Sciences, Jilin University, Jilin 130012, China.
authors.forename: Di
authors.identifier: 0000-0002-7322-4174
authors.initials: D
authors.lastname: Wang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy* / Q000473:pathology; D020169:Caspases / Q000096:biosynthesis; D049109:Cell Proliferation / Q000187:drug effects; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy* / Q000473:pathology; D051379:Mice; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism; D009363:Neoplasm Proteins / Q000096:biosynthesis; D010936:Plant Extracts / Q000008:administration & dosage / Q000737:chemistry; D015398:Signal Transduction / Q000187:drug effects; D055439:Tricholoma / Q000737:chemistry*; D023041:Xenograft Model Antitumor Assays
chemical_list: D009363:Neoplasm Proteins; D010936:Plant Extracts; D020169:Caspases