title: Alkaline Technosol contaminated by former mining activity and its culturable autochthonous microbiota.
abstract: Technosols or technogenic substrates contaminated by potentially toxic elements as a result of iron mining causes not only contamination of the surrounding ecosystem but may also lead to changes of the extent, abundance, structure and activity of soil microbial community. Microbial biomass were significantly inhibited mainly by exceeding limits of potentially toxic metals as arsenic (in the range of 343-511 mg/kg), copper (in the range of 7980-9227 mg/kg), manganese (in the range of 2417-2670 mg/kg), alkaline and strong alkaline pH conditions and minimal contents of organic nutrients. All of the 14 bacterial isolates, belonged to 4 bacterial phyla, Actinobacteria, Firmicutes; β- and γ-Proteobacteria. Thirteen genera and 20 species of microscopic filamentous fungi were recovered. The most frequently found species belonged to genera Aspergillus (A. clavatus, A. niger, A. flavus, A. versicolor, Aspergillus sp.) with the dominating A. niger in all samples, and Penicillium (P. canescens, P. chrysogenum, P. spinulosum, Penicillium sp.). Fungal plant pathogens occurred in all surface samples. These included Bjerkandera adustata, Bionectria ochloleuca with anamorph state Clonostachys pseudochloleuca, Lewia infectoria, Phoma macrostoma and Rhizoctonia sp.
authors.affiliation: Department of Soil Science, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic. Electronic address: asimonovicova@fns.uniba.sk.
authors.forename: A
authors.initials: A
authors.lastname: Šimonovičová
authors.affiliation: Institut of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors.forename: P
authors.initials: P
authors.lastname: Ferianc
authors.affiliation: Institute of Environmental Engineering, Faculty of Mining and Geology, VŠB - Technical University of Ostrava, Czech Republic.
authors.forename: H
authors.initials: H
authors.lastname: Vojtková
authors.affiliation: Institut of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors.forename: D
authors.initials: D
authors.lastname: Pangallo
authors.affiliation: Department of Soil Science, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
authors.forename: P
authors.initials: P
authors.lastname: Hanajík
authors.affiliation: Institut of Molecular Biology, Slovak Academy of Sciences, 845 51 Bratislava, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Kraková
authors.affiliation: Department of Soil Science, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Feketeová
authors.affiliation: Department of Environmental Ecology, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
authors.forename: S
authors.initials: S
authors.lastname: Čerňanský
authors.affiliation: Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
authors.forename: L
authors.initials: L
authors.lastname: Okenicová
authors.affiliation: Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Žemberyová
authors.affiliation: Institute of Laboratory Research on Geomaterials, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
authors.forename: M
authors.initials: M
authors.lastname: Bujdoš
authors.affiliation: Department of Landscape Ecology, Faculty of Natural Sciences, Comenius University, 842 15 Bratislava, Slovak Republic.
authors.forename: E
authors.initials: E
authors.lastname: Pauditšová
mesh_terms: D001151:Arsenic / Q000633:toxicity; D001419:Bacteria / Q000302:isolation & purification; D018533:Biomass; D003300:Copper / Q000633:toxicity; D005658:Fungi / Q000302:isolation & purification; D008345:Manganese / Q000633:toxicity; D064307:Microbiota; D008906:Mining; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000633:toxicity*
chemical_list: D012989:Soil Pollutants; D008345:Manganese; D003300:Copper; D001151:Arsenic
keywords: Alkaline Technosols; Autochthonous isolates; Microbial biomass; Potentially toxic elements