title: Chloroplast Genome Sequence of Pigeonpea (Cajanus cajan(L.) Millspaugh) andCajanus scarabaeoides(L.) Thouars: Genome Organization and Comparison with Other Legumes.
abstract: Pigeonpea (Cajanus cajan(L.) Millspaugh), a diploid (2n = 22) legume crop with a genome size of 852 Mbp, serves as an important source of human dietary protein especially in South East Asian and African regions. In this study, the draft chloroplast genomes ofCajanus cajanandCajanus scarabaeoides(L.) Thouars were generated.Cajanus scarabaeoidesis an important species of theCajanusgene pool and has also been used for developing promising CMS system by different groups. A male sterile genotype harboring theC. scarabaeoidescytoplasm was used for sequencing the plastid genome. The cp genome ofC. cajanis 152,242bp long, having a quadripartite structure with LSC of 83,455 bp and SSC of 17,871 bp separated by IRs of 25,398 bp. Similarly, the cp genome ofC. scarabaeoidesis 152,201bp long, having a quadripartite structure in which IRs of 25,402 bp length separates 83,423 bp of LSC and 17,854 bp of SSC. The pigeonpea cp genome contains 116 unique genes, including 30 tRNA, 4 rRNA, 78 predicted protein coding genes and 5 pseudogenes. A 50 kb inversion was observed in the LSC region of pigeonpea cp genome, consistent with other legumes. Comparison of cp genome with other legumes revealed the contraction of IR boundaries due to the absence ofrps19gene in the IR region. Chloroplast SSRs were mined and a total of 280 and 292 cpSSRs were identified inC. scarabaeoidesandC. cajanrespectively. RNA editing was observed at 37 sites in bothC. scarabaeoidesandC. cajan, with maximum occurrence in thendhgenes. The pigeonpea cp genome sequence would be beneficial in providing informative molecular markers which can be utilized for genetic diversity analysis and aid in understanding the plant systematics studies among major grain legumes.
authors.affiliation: ICAR-National Research Centre on Plant BiotechnologyNew Delhi, India; Department of Bio & Nanotechnology, Guru Jambheshwar University of Science & TechnologyHisar, India.
authors.forename: Tanvi
authors.initials: T
authors.lastname: Kaila
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: Pavan K
authors.initials: PK
authors.lastname: Chaduvla
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: Swati
authors.initials: S
authors.lastname: Saxena
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: Kaushlendra
authors.initials: K
authors.lastname: Bahadur
authors.affiliation: Biotechnology Department, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth Akola, India.
authors.forename: Santosh J
authors.initials: SJ
authors.lastname: Gahukar
authors.affiliation: Department of Bio & Nanotechnology, Guru Jambheshwar University of Science & Technology Hisar, India.
authors.forename: Ashok
authors.initials: A
authors.lastname: Chaudhury
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: T R
authors.initials: TR
authors.lastname: Sharma
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: N K
authors.initials: NK
authors.lastname: Singh
authors.affiliation: ICAR-National Research Centre on Plant Biotechnology New Delhi, India.
authors.forename: Kishor
authors.initials: K
authors.lastname: Gaikwad
keywords: Cajanus cajan; Cajanus scarabaeoides; RNA editing; Roche 454 sequencing; chloroplast genome