title: Functional analysis of HSPA1A and HSPA8 in parturition.
abstract: Many factors are involved in parturition, such as apoptosis, inflammatory mediators, and hormones. Previous studies indicated that HSP70 directly or indirectly regulates apoptosis, inflammatory immune response and hormone stimulus. To gain new insights into molecular mechanism underlying HSP70 for regulating parturition, we overexpressed and knocked down two representative members of HSP70 (HSPA1A and HSPA8) through transfection of their recombinant plasmid and si-RNA separately in WISH (human amniotic epithelial) cells. The expression changes of several pathways' marker genes were investigated by Western blotting and quantitative real-time PCR (qRT-PCR). Results showed extreme expression changes in the genes of IL-8 and ESR2. HSP70 was found to stimulate estrogen response by regulating ESR2 through ERK1/2 after treating WISH cells with the special phosphorylation inhibitor of ERK1/2 and analyzing the changes of E2 concentration by ELISA. HSP70 was also observed to contribute to preterm birth after administering the special inhibitor of HSP70-PFT-μ with LPS-induced preterm birth mouse model. Overall, HSP70 induces parturition through stimulating immune inflammatory and estrogen response. The balanced HSP70 expression could ensure a smooth parturition, while the imbalanced expression may cause a pathological state like preterm.
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
authors.forename: Junnan
authors.initials: J
authors.lastname: Geng
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
authors.forename: Huanan
authors.initials: H
authors.lastname: Li
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Huang
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chai
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Fenge
authors.initials: F
authors.lastname: Li
authors.affiliation: Key Laboratory of Swine Genetics and Breeding of Agricultural Ministry, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, China; The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, China. Electronic address: jiangsiwen@mail.hzau.edu.cn.
authors.forename: Siwen
authors.initials: S
authors.lastname: Jiang
mesh_terms: D000650:Amnion / Q000378:metabolism; D000818:Animals; D017209:Apoptosis; D004847:Epithelial Cells / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism*; D005260:Female; D050883:HSC70 Heat-Shock Proteins / Q000502:physiology*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism / Q000502:physiology*; D006801:Humans; D007107:Immune System; D007249:Inflammation; D016209:Interleukin-8 / Q000378:metabolism; D020935:MAP Kinase Signaling System; D051379:Mice; D008807:Mice, Inbred BALB C; D036801:Parturition / Q000378:metabolism*; D010766:Phosphorylation; D011487:Protein Conformation; D011897:Random Allocation; D011994:Recombinant Proteins / Q000378:metabolism
chemical_list: C508606:CXCL8 protein, human; C000600034:ESR2 protein, human; D047629:Estrogen Receptor beta; D050883:HSC70 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C490786:HSPA1A protein, human; C494371:HSPA8 protein, human; C498550:Hsp70.3 protein, mouse; C494372:Hspa8 protein, mouse; D016209:Interleukin-8; D011994:Recombinant Proteins
keywords: ERK1/2; ESR2; HSP70; IL-8; PFT-μ; Parturition