title: FAM98A associates with DDX1-C14orf166-FAM98B in a novel complex involved in colorectal cancer progression.
abstract: Protein Arginine Methyl Transferase 1 (PRMT1) is deemed to be a potential oncogenic protein considering its overexpression in several malignancies including colorectal cancer. However, the molecular pathogenesis regarding PRMT1 overexpression and overall poor patient survival involved in this devastating and life threatening cancer remains obscured. In our previous study, we have identified FAM98A as a novel substrate of PRMT1 and also identified its role in ovarian cancer progression. Here, we showed that the two structural homologs FAM98A and FAM98B included in a novel complex with DDX1 and C14orf166 are required for PRMT1 expression. Analysis of the data from The Cancer Genome Atlas (TCGA) database and clinical colorectal cancer specimens also demonstrated a strong positive correlation and co-occurrence of PRMT1, FAM98A and FAM98B. These findings provide a mechanistic insight into how knockdown of FAM98A or FAM98B can suppress the malignant characteristics of cancer cells. Besides, we showed that FAM98A and FAM98B are working in the same axis as knockdown of both proteins together does not cause additional reduction in the cellular proliferation and colony formation of colorectal cancer cells.
authors.affiliation: Department of Pharmaceutical Sciences, North South University Bangladesh, Dhaka 1229, Bangladesh. Electronic address: ayash_8@yahoo.com.
authors.forename: Khondker Ayesha
authors.initials: KA
authors.lastname: Akter
authors.affiliation: Biochemistry Division, Department of Chemistry, Faculty of Science, Tanta University, Tanta 31527, Egypt. Electronic address: biomansour@hotmail.com.
authors.forename: Mohammed A
authors.initials: MA
authors.lastname: Mansour
authors.affiliation: Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya 466-8550, Japan.
authors.forename: Toshinori
authors.initials: T
authors.lastname: Hyodo
authors.affiliation: Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai, Showa, Nagoya 466-8550, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Senga
mesh_terms: D002352:Carrier Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000209:etiology / Q000235:genetics / Q000378:metabolism*; D053487:DEAD-box RNA Helicases / Q000378:metabolism*; D018450:Disease Progression; D055785:Gene Knockdown Techniques; D045325:HCT116 Cells; D019073:HT29 Cells; D006801:Humans; D046912:Multiprotein Complexes / Q000378:metabolism; D011484:Protein-Arginine N-Methyltransferases / Q000235:genetics / Q000378:metabolism*; D011506:Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012334:RNA, Neoplasm / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000235:genetics / Q000378:metabolism*; D015534:Trans-Activators / Q000378:metabolism*; D014410:Tumor Stem Cell Assay
chemical_list: D002352:Carrier Proteins; C000613973:FAM98A protein, human; C000595051:FAM98B protein, human; D046912:Multiprotein Complexes; D011506:Proteins; D012333:RNA, Messenger; D012334:RNA, Neoplasm; C435504:RTRAF protein, human; D012097:Repressor Proteins; D015534:Trans-Activators; C099988:PRMT1 protein, human; D011484:Protein-Arginine N-Methyltransferases; C086252:DDX1 protein, human; D053487:DEAD-box RNA Helicases
keywords: Colorectal cancer; FAM98A; FAM98B; PRMT1