title: In vivo Confocal Raman Spectroscopic Analysis of the Effects of Infrared Radiation in the Human Skin Dermis.
abstract: Human skin is the outer covering of the body, and its composition changes with overexposure to environmental pollution and solar radiation. Infrared (IR) radiation is capable of penetrating more deeply into the skin producing free radicals causing irreversible damage. Confocal Raman spectroscopy was considered as a potential tool for the in vivo analysis of the different metabolic conditions with respect to different depths of the skin. In this regard, this work verifies the influence of infrared radiation on the skin dermis after having been exposed to 432 J cm-2which corresponds to the dose received in a day in the summer time in a tropical region. This study was performed with 17 female volunteers who were divided into two groups. The marked skin area was exposed twice to IR radiation for a duration of 30 min each with an interval of 30 min. The spectral signatures were collected in the fingerprint region before (T0) and after 60 min (T60) of IR irradiation. The analysis shows that, on average, no significant variations occurred in group I and decreased collagen was observed in group II. However, when considering the effect seen in each individual, collagen degradation was detected in 60% of volunteers.
authors.affiliation: Laboratory of Biomedical Vibrational Spectroscopy, Institute of Research and Development, University of Paraiba Valley (UNIVAP), São José dos Campos, Brazil.
authors.forename: Monica Bergamo
authors.initials: MB
authors.lastname: Lopes
authors.affiliation: Laboratory of Biomedical Vibrational Spectroscopy, Institute of Research and Development, University of Paraiba Valley (UNIVAP), São José dos Campos, Brazil.|Department of Physics, Bannari Amman Institute of Technology, Sathyamangalam, India.
authors.forename: Ramu
authors.initials: R
authors.lastname: Rajasekaran
authors.affiliation: Laboratory of Biomedical Vibrational Spectroscopy, Institute of Research and Development, University of Paraiba Valley (UNIVAP), São José dos Campos, Brazil.
authors.forename: Ana Clara Figueira
authors.initials: AC
authors.lastname: Lopes Cançado
authors.affiliation: Biomedical Engineering Innovation Center, Universidade Brazil, Itaquera, Sao Paulo, Brazil.|Universidade Federal do Piaui, Departamento de Fisica, Teresina, Brazil.
authors.forename: Airton Abrahão
authors.initials: AA
authors.lastname: Martin
mesh_terms: D000328:Adult; D020405:Dermis / Q000528:radiation effects*; D005260:Female; D006801:Humans; D007259:Infrared Rays; D008875:Middle Aged; D013059:Spectrum Analysis, Raman / Q000379:methods*; D055815:Young Adult