title: Direct detection of cysteine using functionalized BaTiO3nanoparticles film based self-powered biosensor.
abstract: Simple, novel, and direct detection of clinically important biomolecules have continuous demand among scientific community as well as in market. Here, we report the first direct detection and facile fabrication of a cysteine-responsive, film-based, self-powered device. NH2functionalized BaTiO3nanoparticles (BT-NH2NPs) suspended in a three-dimensional matrix of an agarose (Ag) film, were used for cysteine detection. BaTiO3nanoparticles (BT NPs) semiconducting as well as piezoelectric properties were harnessed in this study. The changes in surface charge properties of the film with respect to cysteine concentrations were determined using a current-voltage (I-V) technique. The current response increased with cysteine concentration (linear concentration range=10µM-1mM). Based on the properties of the composite (BT/Ag), we created a self-powered cysteine sensor in which the output voltage from a piezoelectric nanogenerator was used to drive the sensor. The potential drop across the sensor was measured as a function of cysteine concentrations. Real-time analysis of sensor performance was carried out on urine samples by non-invasive method. This novel sensor demonstrated good selectivity, linear concentration range and detection limit of 10µM; acceptable for routine analysis.
authors.affiliation: Nanomaterials and System Lab, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Sophia
authors.initials: S
authors.lastname: Selvarajan
authors.affiliation: Nanomaterials and System Lab, Department of Mechanical Engineering, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Nagamalleswara Rao
authors.initials: NR
authors.lastname: Alluri
authors.affiliation: Nanomaterials and System Lab, Department of Mechatronics Engineering, Jeju National University, Jeju 690-756, Republic of Korea.
authors.forename: Arunkumar
authors.initials: A
authors.lastname: Chandrasekhar
authors.affiliation: Nanomaterials and System Lab, Department of Advanced Convergence Technology and Science, Jeju National University, Jeju 690-756, Republic of Korea; Nanomaterials and System Lab, Department of Mechatronics Engineering, Jeju National University, Jeju 690-756, Republic of Korea. Electronic address: kimsangj@jejunu.ac.kr.
authors.forename: Sang-Jae
authors.initials: SJ
authors.lastname: Kim
mesh_terms: D017609:Barium Compounds / Q000737:chemistry*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D003545:Cysteine / Q000652:urine*; D004560:Electricity; D004867:Equipment Design; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D012685:Sepharose / Q000737:chemistry*; D014025:Titanium / Q000737:chemistry*
chemical_list: D017609:Barium Compounds; C024547:barium titanate(IV); D012685:Sepharose; D014025:Titanium; D003545:Cysteine
keywords: Agarose 3D matrix; I–V technique; NH(2)-functionalized BaTiO(3) NPs; Non-invasive (urine); Piezoelectric nanogenerator; Self-powered cysteine bio-sensor