title: Assays for Identifying Inducers of the Antimicrobial Peptide LL-37.
abstract: One promising approach to meet the growing problem of antibiotic resistance is to modulate host defense mechanisms, i.e., host-directed therapy (HDT), in the fight against infections. Induction of endogenous antimicrobial peptides (AMPs) via small molecular compounds, such as 1,25-dihydroxyvitamin D3or phenylbutyrate, could provide one such HDT-based approach.We have developed a cell-based screening assay for the identification of novel compounds with the capacity to induce AMP expression and here follows the detailed protocol.
authors.affiliation: Department of Laboratory Medicine, Karolinska Institutet, F68, Karolinska Universitetssjukhuset, Huddinge, 141 86, Sweden. frank.nylen@ki.se.
authors.forename: Frank
authors.initials: F
authors.lastname: Nylén
authors.affiliation: Department of Laboratory Medicine, Karolinska Institutet, F68, Karolinska Universitetssjukhuset, Huddinge, 141 86, Sweden.
authors.forename: Peter
authors.initials: P
authors.lastname: Bergman
authors.affiliation: Institute of Biology and Biomedical Center, University of Iceland, Reykjavík, Iceland.
authors.forename: Gudmundur H
authors.initials: GH
authors.lastname: Gudmundsson
authors.affiliation: Department of Laboratory Medicine, Karolinska Institutet, F68, Karolinska Universitetssjukhuset, Huddinge, 141 86, Sweden.
authors.forename: Birgitta
authors.initials: B
authors.lastname: Agerberth
mesh_terms: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D001681:Biological Assay / Q000379:methods*; D054804:Cathelicidins / Q000235:genetics*; D002460:Cell Line; D055808:Drug Discovery / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005786:Gene Expression Regulation / Q000187:drug effects*; D023061:Gene Order; D017930:Genes, Reporter; D005822:Genetic Vectors / Q000235:genetics; D006801:Humans; D054852:Small Molecule Libraries; D014162:Transfection
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins; D054852:Small Molecule Libraries
keywords: CAMP gene; Cell-based reporter system; Chemical library screening; Z′-factor