title: DNA biosensing with 3D printing technology.
abstract: 3D printing, an upcoming technology, has vast potential to transform conventional fabrication processes due to the numerous improvements it can offer to the current methods. To date, the employment of 3D printing technology has been examined for applications in the fields of engineering, manufacturing and biological sciences. In this study, we examined the potential of adopting 3D printing technology for a novel application, electrochemical DNA biosensing. Metal 3D printing was utilized to construct helical-shaped stainless steel electrodes which functioned as a transducing platform for the detection of DNA hybridization. The ability of electroactive methylene blue to intercalate into the double helix structure of double-stranded DNA was then exploited to monitor the DNA hybridization process, with its inherent reduction peak serving as an analytical signal. The designed biosensing approach was found to demonstrate superior selectivity against a non-complementary DNA target, with a detection range of 1-1000 nM.
authors.affiliation: Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore. pumera.research@gmail.com.
authors.forename: Adeline Huiling
authors.initials: AH
authors.lastname: Loo
authors.affiliation: Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore. pumera.research@gmail.com.
authors.forename: Chun Kiang
authors.initials: CK
authors.lastname: Chua
authors.affiliation: Division of Chemistry & Biological Chemistry, School of Physical and Mathematical Sciences, Nanyang Technological University, 21 Nanyang Link, Singapore 637371, Singapore. pumera.research@gmail.com.
authors.forename: Martin
authors.identifier: 0000-0001-5846-2951
authors.initials: M
authors.lastname: Pumera
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques / Q000379:methods; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D007364:Intercalating Agents / Q000737:chemistry; D057230:Limit of Detection; D008751:Methylene Blue / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D066330:Printing, Three-Dimensional
chemical_list: D007364:Intercalating Agents; D006046:Gold; D004247:DNA; D008751:Methylene Blue