title: Functional characterization of the cutI gene for the transcription of carbon monoxide dehydrogenase genes in Mycobacterium sp. strain JC1 DSM 3803.
abstract: Carbon monoxide dehydrogenase (CO-DH) in Mycobacterium sp. strain JC1 is a key enzyme for the carboxydotrophic growth, when carbon monoxide (CO) is supplied as a sole source of carbon and energy. This enzyme is also known to act as nitric oxide dehydrogenase (NO-DH) for the detoxification of NO. Several accessory genes such as cutD, cutE, cutF, cutG, cutH, and cutI, are clustered together with two copies of the CO-DH structural genes (cutB1C1A1 and cutB2C2A2) in Mycobacterium sp. strain JC1 and are well conserved in carboxydotrophic mycobacteria. Transcription of the CO-DH structural and accessory genes was demonstrated to be increased significantly by acidified sodium nitrate as a source of NO. A cutI deletion (ΔcutI) mutant of Mycobacterium sp. strain JC1 was generated to identity the function of CutI. Lithoautotrophic growth of the ΔcutI mutant was severely affected in mineral medium supplemented with CO, while the mutant grew normally with glucose. Western blotting, CO-DH activity staining, and CO-DH-specific enzyme assay revealed a significant decrease in the cellular level of CO-DH in the ΔcutI mutant. Northern blot analysis and promoter assay showed that expression of the cutB1 and cutB2 genes was significantly reduced at the transcriptional level in the ΔcutI mutant, compared to that of the wildtype strain. The ΔcutI mutant was much more susceptible to NO than was the wild type.
authors.affiliation: Department of Systems Biology, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Jae Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Systems Biology, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Sae Woong
authors.initials: SW
authors.lastname: Park
authors.affiliation: Department of Systems Biology, Yonsei University, Seoul, 03722, Republic of Korea.
authors.forename: Young Min
authors.initials: YM
authors.lastname: Kim
authors.affiliation: Department of Microbiology, Pusan National University, Busan, 46241, Republic of Korea. joh@pusan.ac.kr.
authors.forename: Jeong-Il
authors.initials: JI
authors.lastname: Oh
mesh_terms: D000445:Aldehyde Oxidoreductases / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D001426:Bacterial Proteins / Q000737:chemistry / Q000235:genetics*; D002248:Carbon Monoxide / Q000378:metabolism; D003001:Cloning, Molecular; D003470:Culture Media; D017353:Gene Deletion; D005798:Genes, Bacterial; D005947:Glucose / Q000378:metabolism; D009097:Multienzyme Complexes / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D009161:Mycobacterium / Q000187:drug effects / Q000201:enzymology / Q000235:genetics* / Q000254:growth & development; D009566:Nitrates / Q000378:metabolism; D009569:Nitric Oxide / Q000378:metabolism / Q000494:pharmacology; D014158:Transcription, Genetic
chemical_list: D001426:Bacterial Proteins; D003470:Culture Media; D009097:Multienzyme Complexes; D009566:Nitrates; D009569:Nitric Oxide; D002248:Carbon Monoxide; C031618:sodium nitrate; D000445:Aldehyde Oxidoreductases; C019823:carbon monoxide dehydrogenase; D005947:Glucose
keywords: CO dehydrogenase; CutI; Mycobacterium sp. strain JC1; carboxydobacteria; nitric oxide