title: Development and Evaluation of Event-Specific Quantitative PCR Method for Genetically Modified Soybean MON87701.
abstract: A real-time PCR-based analytical method was developed for the event-specific quantification of a genetically modified (GM) soybean event, MON87701. First, a standard plasmid for MON87701 quantification was constructed. The conversion factor (Cf) required to calculate the amount of genetically modified organism (GMO) was experimentally determined for a real-time PCR instrument. The determined Cffor the real-time PCR instrument was 1.24. For the evaluation of the developed method, a blind test was carried out in an inter-laboratory trial. The trueness and precision were evaluated as the bias and reproducibility of relative standard deviation (RSDr), respectively. The determined biases and the RSDr values were less than 30 and 13%, respectively, at all evaluated concentrations. The limit of quantitation of the method was 0.5%, and the developed method would thus be applicable for practical analyses for the detection and quantification of MON87701.
authors.affiliation: Division of Analytical Science, Food Research Institute, National Agriculture and Food Research Organization.
authors.forename: Keita
authors.initials: K
authors.lastname: Tsukahara
authors.forename: Reona
authors.initials: R
authors.lastname: Takabatake
authors.forename: Tomoko
authors.initials: T
authors.lastname: Masubuchi
authors.forename: Satoshi
authors.initials: S
authors.lastname: Futo
authors.forename: Yasutaka
authors.initials: Y
authors.lastname: Minegishi
authors.forename: Akio
authors.initials: A
authors.lastname: Noguchi
authors.forename: Kazunari
authors.initials: K
authors.lastname: Kondo
authors.forename: Tomoko
authors.initials: T
authors.lastname: Nishimaki-Mogami
authors.forename: Takeyo
authors.initials: T
authors.lastname: Kurashima
authors.forename: Junichi
authors.initials: J
authors.lastname: Mano
authors.forename: Kazumi
authors.initials: K
authors.lastname: Kitta
mesh_terms: D001483:Base Sequence; D005504:Food Analysis / Q000379:methods*; D030841:Food, Genetically Modified; D030781:Organisms, Genetically Modified; D016133:Polymerase Chain Reaction / Q000379:methods*; D015203:Reproducibility of Results; D013025:Glycine max / Q000235:genetics