title: The significant effect of chronic intermittent hypoxia on prostaglandin D2biosynthesis in rat brain.
abstract: Obstructive sleep apnea (OSA) is a common disorder characterized by chronic intermittent hypoxia (CIH). Excessive daytime sleepiness (EDS) is one of severe complications frequently associated with OSA. Lipocalin-type prostaglandin synthase (L-PGDS) is potentially responsible for the production of prostaglandin D2(PGD2) which is an endogenous sleep inducer. To date, whether the content of PGD2and PGDS is related to intermittent hypoxia has never been reported. The aim of this study was to compare the content of PGD2and L-PGDS in rats' brains with and without intermittent hypoxia. Adult male Wistar rats (n = 48; 8-10 weeks) were averagely divided into two groups. One was control group, and the other group was exposed to IH (12 h/day for 6 weeks). In each group there are four time-points including 0, 2, 4 and 6 weeks, and six rats were killed and studied at each time-point. At the end of 0, 2, 4 and 6 weeks, the concentrations of PGD2in brains were measured by LC-MS/MS. In addition, the expressions of L-PGDS protein and mRNA in brains were investigated by western blotting and real-time polymerase chain reaction (RT-PCR), respectively. The results showed the concentrations of PGD2in CIH rat brains were higher than those in control groups from the second week. At the end of 6 weeks, the concentrations of PGD2in CIH and control groups were 11.1 and 5.9 ng/g, respectively. The levels of L-PGDS protein and mRNA followed the same trend during the whole 6 weeks. The results will provide a new idea to explore that patients with OSA are always accompanied by excessive daytime sleepiness.
authors.affiliation: Department of Respiratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121001, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Shan
authors.affiliation: Department of Respiratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121001, China.
authors.forename: Wen-Shu
authors.initials: WS
authors.lastname: Chai
authors.affiliation: Department of Respiratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121001, China.
authors.forename: Si-Jing
authors.initials: SJ
authors.lastname: Lu
authors.affiliation: National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, 330004, China.
authors.forename: Yong-Gui
authors.initials: YG
authors.lastname: Song
authors.affiliation: National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, Jiangxi, 330004, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Su
authors.affiliation: Department of Internal Medicine, University of Manitoba, Winnipeg, Canada; Department of Community Health Sciences, University of Manitoba, Winnipeg, Canada.
authors.forename: Shuman
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou, Liaoning, 121001, China. Electronic address: sxbsln@163.com.
authors.forename: Xian-Bao
authors.initials: XB
authors.lastname: Shi
authors.affiliation: Institute of Respiratory Disease, The First Hospital of China Medical University, Shenyang, Liaoning, 110001, China. Electronic address: wwbycmu2@126.com.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D000860:Hypoxia / Q000503:physiopathology*; D019746:Intramolecular Oxidoreductases / Q000096:biosynthesis*; D054834:Lipocalins / Q000096:biosynthesis*; D008297:Male; D015230:Prostaglandin D2 / Q000096:biosynthesis*; D011451:Prostaglandin-Endoperoxide Synthases / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results; D012890:Sleep / Q000502:physiology; D053719:Tandem Mass Spectrometry
chemical_list: D054834:Lipocalins; D011451:Prostaglandin-Endoperoxide Synthases; D019746:Intramolecular Oxidoreductases; C022466:prostaglandin R2 D-isomerase; D015230:Prostaglandin D2
keywords: Chronic intermittent hypoxia; LC-MS/MS; Prostaglandin D(2); Prostaglandin D(2) synthase; Sleep apnea