title: Peptaibol profiles of Iranian Trichoderma isolates.
abstract: Five Iranian Trichoderma isolates from species T. viride, T. viridescens, T. asperellum, T. longibrachiatum and T. citrinoviride - selected from the Fungal Collection of the Bu Ali Sina University, Hamedan, Iran - were investigated for their peptaibol production. All examined isolates showed remarkable antibacterial activities during the screening of their extracts for peptaibol content with a Micrococcus luteus test culture. HPLC-ESI-IT MS was used for identification and elucidation of the amino acid sequences of peptaibols. The detected peptaibol compounds contain 20 or 18 amino acid residues and belong to the trichobrachin and trichotoxin groups of peptaibols, respectively. T. longibrachiatum and T. citrinoviride produced trichobrachins, while trichotoxins could be detected in T. viride, T. viridescens and T. asperellum. Out of 37 sequences detetermined, 26 proved to be new, yet undescribed compounds, while others were identified as previously reported trichotoxins (trichotoxin A-50s and T5D2) and trichobrachins (longibrachins AI, AII, AIII, BII and BIII). Compounds within the two groups of detected peptaibols differed from each other only by a single or just a few amino acid changes.
authors.affiliation: Department of Plant Protection, Bu Ali Sina University , Hamedan , Iran.
authors.forename: Parisa Rahimi
authors.initials: PR
authors.lastname: Tamandegani
authors.affiliation: Department of Plant Protection, Bu Ali Sina University , Hamedan , Iran.
authors.forename: Doustmorad
authors.initials: D
authors.lastname: Zafari
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged , Szeged , Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Marik
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged , Szeged , Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Szekeres
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged , Szeged , Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Vágvölgyi
authors.affiliation: Department of Microbiology, Faculty of Science and Informatics, University of Szeged , Szeged , Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Kredics
mesh_terms: D000890:Anti-Infective Agents / Q000378:metabolism / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides; D002851:Chromatography, High Pressure Liquid; D007492:Iran; D016982:Micrococcus luteus / Q000187:drug effects; D054848:Peptaibols / Q000378:metabolism* / Q000494:pharmacology; D010455:Peptides / Q000378:metabolism / Q000494:pharmacology; D021241:Spectrometry, Mass, Electrospray Ionization; D014242:Trichoderma / Q000378:metabolism*
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D054848:Peptaibols; D010455:Peptides; C402113:longibrachin LGA I; C433572:longibrachin LGB II; C433573:longibrachin LGB III; C016161:trichotoxin
keywords: Trichoderma; antimicrobial activity; electrospray ionization mass spectrometry; high performance liquid chromatography; peptaibol