title: Genetic structure and historical demography of the blue swimming crab (Portunus pelagicus) from southeastern sea of China based on mitochondrial COI gene.
abstract: In this study, the population genetic structure and historical demography of the blue swimming crab, Portunus pelagicus, from southeastern sea of China were investigated using cytochrome c oxidase subunit I (COI) gene of mitochondrion. A total of 889 bp segment of COI gene was sequenced, which showed a high haplotype diversity (0.6833-0.8142) and low nucleotide diversity (0.0021-0.0034). Among 30 haplotypes defined in this study, one (H1) was the most dominant (47.7%) and shared by each locality, while the majority (23) were rare and only existed in one individual. The AMOVA analysis revealed a limited population genetic structure, which suggested a high level of gene flow along the distribution areas of China. This conclusion was supported by the pairwise FSTcomparison values. The topology of the neighbour-joining tree constructed using 30 haplotypes from four localities presented two distinct clades (clade A and clade B). Meanwhile, three sequences of P. pelagicus downloaded from NCBI database showed a high-level divergence with the individuals collected in our study, which might form a new cryptical species. The individuals of clade B were cryptically embedded in the whole population, with a low frequency (7.7-24.2%), while clade A accounted for 75.8-92.3%. Neutrality tests and mismatch analyses suggested a late Pleistocene population expansion for both clade A (47,000-66,000 years ago) and clade B (74,000-100,000 years ago). This study should provide insight into phylogeny, population genetic structure, conservation genetics, and sustainable management of P. pelagicus.
authors.affiliation: a Guangdong Provincial Key Laboratory of Marine Biotechnology , Shantou University , Shantou , China.|b East China Sea Fisheries Research Institute , Chinese Academy of Fishery Sciences , Shanghai , China.
authors.forename: Guijing
authors.initials: G
authors.lastname: Ren
authors.affiliation: a Guangdong Provincial Key Laboratory of Marine Biotechnology , Shantou University , Shantou , China.|c College of Biology and Chemistry, Xingyi Normal University for Nationalities , Xingyi, China.
authors.forename: Guidong
authors.initials: G
authors.lastname: Miao
authors.affiliation: b East China Sea Fisheries Research Institute , Chinese Academy of Fishery Sciences , Shanghai , China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Ma
authors.affiliation: b East China Sea Fisheries Research Institute , Chinese Academy of Fishery Sciences , Shanghai , China.
authors.forename: Jianxue
authors.initials: J
authors.lastname: Lu
authors.affiliation: a Guangdong Provincial Key Laboratory of Marine Biotechnology , Shantou University , Shantou , China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Yang
authors.affiliation: a Guangdong Provincial Key Laboratory of Marine Biotechnology , Shantou University , Shantou , China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Ma
mesh_terms: D000818:Animals; D003386:Brachyura / Q000145:classification* / Q000235:genetics; D002681:China; D003576:Electron Transport Complex IV / Q000235:genetics*; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D010802:Phylogeny; D011157:Population Dynamics; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D003576:Electron Transport Complex IV
keywords: COI gene; Portunus pelagicus; historical demography; phylogeney; population genetic structure