title: Transcriptional abundance of antioxidant enzymes in endometrium and their circulating levels in Zebu cows with and without uterine infection.
abstract: Oxidative stress during peripartum period may compromise the uterine immunity. In the present study, we assessed the oxidative stress and antioxidant status during peripartum period and studied their relationship with postpartum uterine infection in dairy cows. Peripheral blood concentrations of total antioxidant capacity (TAC), malondialdehyde (MDA) and nitric oxide (NO) were determined (day -21, -7, on the day of calving and day +7, +21, +35) in normal (n=11), puerperal metritic (n=7) and clinical endometritic (n=6) cows. Endometrial biopsy was performed on the day of calving and expression of CAT, GPx4 and SOD2 genes was studied using qRT-PCR. Puerperal metritic cows had significantly (P<0.05) lower TAC (on day -7, day 0, day +7, +21 & +35), higher MDA (on day -21, -7 & on the day of calving) and NO (on day 0, +7 & day +35) concentrations compared to normal cows. Similarly, clinical endometritic cows had significantly (P<0.05) lower TAC (on day -7, 0, +7 & +21), higher MDA (on day -21, -7, +7 and +35) and NO (on day +7, +21 & +35) concentrations compared to normal cows. The expression of CAT and GPx4 genes was lower (P<0.05) and SOD2 gene was higher (P<0.05) in endometrial tissue of cows that developed uterine infection compared to normal cows. The relationship of peripheral levels of MDA and NO with antioxidant enzymes expression in endometrial tissue was found significant. Receiver operator characteristic analysis revealed that the concentrations of TAC on day -7 to day +35, MDA on day -21 to day +7 and NO on the day of calving to day +35 were highly correlated to the development of postpartum uterine infection in cows. It may be inferred that the low serum TAC level and high level of lipid peroxidation and NO during peripartum period influenced the endometrial expression of anitioxidative genes that compromised the uterine health during postpartum period.
authors.affiliation: Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal 132001, Haryana, India. Electronic address: rbaithalu@gmail.com.
authors.forename: R K
authors.initials: RK
authors.lastname: Baithalu
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, UP, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Singh
authors.affiliation: Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal 132001, Haryana, India. Electronic address: ogkumaresan@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Kumaresan
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: A K
authors.initials: AK
authors.lastname: Mohanty
authors.affiliation: Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: T K
authors.initials: TK
authors.lastname: Mohanty
authors.affiliation: Animal Biotechnology Center, National Dairy Research Institute, Karnal 132001, Haryana, India.
authors.forename: S
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Livestock Production Management, National Dairy Research Institute, Karnal 132 001, Haryana, India.
authors.forename: S
authors.initials: S
authors.lastname: Kerketta
authors.affiliation: Regional Research Center (LUVAS), Veterinary Subunit, Uchani, Karnal, Haryana, 132 001, India.
authors.forename: B R
authors.initials: BR
authors.lastname: Maharana
authors.affiliation: Livestock Production Management, National Dairy Research Institute, Karnal 132 001, Haryana, India.
authors.forename: T K
authors.initials: TK
authors.lastname: Patbandha
authors.affiliation: Livestock Production Management, National Dairy Research Institute, Karnal 132 001, Haryana, India.
authors.forename: N
authors.initials: N
authors.lastname: Attupuram
authors.affiliation: Division of Animal Reproduction, Indian Veterinary Research Institute, Izatnagar, UP, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Agarwal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D001774:Blood Chemical Analysis / Q000662:veterinary; D002417:Cattle; D002418:Cattle Diseases / Q000097:blood / Q000235:genetics; D004716:Endometritis / Q000097:blood / Q000235:genetics; D004717:Endometrium / Q000737:chemistry* / Q000378:metabolism; D004798:Enzymes / Q000097:blood / Q000235:genetics; D005260:Female; D015227:Lipid Peroxidation / Q000235:genetics; D008315:Malondialdehyde / Q000097:blood; D009569:Nitric Oxide / Q000097:blood; D018384:Oxidative Stress / Q000235:genetics*; D058725:Peripartum Period / Q000097:blood / Q000235:genetics; D049590:Postpartum Period / Q000097:blood / Q000235:genetics; D011247:Pregnancy; D011251:Pregnancy Complications, Infectious / Q000097:blood / Q000235:genetics; D011644:Puerperal Disorders / Q000097:blood / Q000235:genetics / Q000662:veterinary; D012333:RNA, Messenger / Q000032:analysis; D014591:Uterine Diseases / Q000097:blood / Q000235:genetics
chemical_list: D000975:Antioxidants; D004798:Enzymes; D012333:RNA, Messenger; D009569:Nitric Oxide; D008315:Malondialdehyde
keywords: Antioxidative molecules; Catalase; Cows; Glutathione peroxidase; Nitric oxide; Oxidative molecules; Peripartum; Superoxide dismutase; Uterine health