title: Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor.
abstract: DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (UA1,UA6andUA7) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible,1H NMR and13C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 × 103, 2.4 × 103and 0.2 × 103M-1, forUA1,UA6andUA7, respectively. Upon binding with DNA, there was a change in colour (a blue shift in theλmaxvalue) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0 ng µl-1forUA1,UA6andUA7, respectively.
authors.affiliation: Department of Chemistry , University of Gujrat , Hafiz Hayat Campus, Gujrat 50700 , Pakistan.
authors.forename: Ataf Ali
authors.identifier: 0000-0001-8018-5890
authors.initials: AA
authors.lastname: Altaf
authors.affiliation: Department of Chemistry , Government College University , Faisalabad 38000 , Pakistan.
authors.forename: Uzma
authors.initials: U
authors.lastname: Hashmat
authors.affiliation: Department of Chemistry , Government College University , Faisalabad 38000 , Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Yousaf
authors.affiliation: Department of Energy Systems Engineering , Sukkur Institute of Business Administration , Sukkur , Pakistan.
authors.forename: Bhajan
authors.initials: B
authors.lastname: Lal
authors.affiliation: Department of Chemistry , Quaid-i-Azam University , Islamabad 45320 , Pakistan.
authors.forename: Shafiq
authors.initials: S
authors.lastname: Ullah
authors.affiliation: Department of Chemistry and Biochemistry , Old-Dominion University , Norfolk , USA.
authors.forename: Alvin A
authors.initials: AA
authors.lastname: Holder
authors.affiliation: Department of Chemistry , Quaid-i-Azam University , Islamabad 45320 , Pakistan.
authors.forename: Amin
authors.initials: A
authors.lastname: Badshah
keywords: DNA binding constant; DNA sensor; azo-guanidine; synthesis