title: Long-Term Quality Control Program Plan for Cord Blood Banks in Korea: A Pilot Study for Cryopreservation Stability.
abstract: BACKGROUND
  Maintaining the quality of cryopreserved cord blood is crucial. In this pilot study, we describe the results of the internal quality control program for a cord blood bank thus far.
  
  
  METHODS
  Donated cord blood units unsuitable for transplantation were selected for internal quality control once a month. One unit of cord blood, aliquoted into 21 capillaries, was cryopreserved and thawed annually to analyze the total nucleated cell count, CD34⁺ cell count, cell viability test, and colony-forming units assay.
  
  
  RESULTS
  No significant differences in the variables (total nucleated cell count, cell viability, CD34⁺ cell count) were observed between samples cryopreserved for one and two years. Upon comparing the variables before cryopreservation and post thawing with the capillaries of one year of storage, cell viability and CD34⁺ cell counts decreased significantly. The use of cord blood samples in capillaries, which can be easily stored for a long period, was similar to the methods used for testing segments attached to the cord blood unit.
  
  
  CONCLUSIONS
  The results of this study may be useful for determining the period during which the quality of cryopreserved cord blood units used for transplantation is maintained.
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.|Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.
authors.forename: Soo Hyun
authors.initials: SH
authors.lastname: Seo
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.|Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.
authors.forename: Sue
authors.initials: S
authors.lastname: Shin
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.|Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea.
authors.forename: Eun Youn
authors.initials: EY
authors.lastname: Roh
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Eun Young
authors.initials: EY
authors.lastname: Song
authors.affiliation: Department of Biostatics, Boramae Hospital, Seoul, Korea.
authors.forename: Sohee
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Obstetrics and Gynecology, Boramae Hospital, Seoul, Korea.
authors.forename: Byoung Jae
authors.initials: BJ
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul, Korea.|Department of Laboratory Medicine, Boramae Hospital, Seoul, Korea.|Seoul Metropolitan Government Public Cord Blood Bank (Allcord), Seoul, Korea. slice@snu.ac.kr.
authors.forename: Jong Hyun
authors.initials: JH
authors.lastname: Yoon
mesh_terms: D018952:Antigens, CD34 / Q000378:metabolism; D001771:Blood Banks; D002452:Cell Count; D002470:Cell Survival; D015925:Cryopreservation / Q000592:standards*; D005312:Fetal Blood / Q000166:cytology*; D006801:Humans; D010865:Pilot Projects; D011786:Quality Control; D056910:Republic of Korea; D013997:Time Factors
chemical_list: D018952:Antigens, CD34
keywords: Capillaries; Cryopreservation; Internal quality control; Umbilical cord blood