title: Hollow fiber liquid-phase microextraction-gas chromatography-mass spectrometry method to analyze bisphenol A and other plasticizer metabolites.
abstract: Phthalates and bisphenol A are important environmental pollutants due to their toxicity for humans and animals, including actions in the endocrine system. Their metabolites in urine can be used as biomarkers to assess human exposure. This paper describes the development of a new method to determine bisphenol A and eight phthalate metabolites in urine samples using hollow fiber liquid phase microextraction (HF-LPME) and gas chromatography-mass spectrometry (GC-MS). This method showed linearity, precision, limits of detection, and quantification suitable to analyze these compounds at low concentration levels in urine. Limits of detection ranged from 0.777 to 23.3μgL-1, showing sensitivity for evaluating environmental exposure. Relative standard deviation (RSD) ranged from 11.7 to 19.7%. The developed method presented a good biomarker alternative for evaluating environmental exposure to bisphenol A and phthalates.
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627-Belo Horizonte/MG, 31270901, Brazil.
authors.forename: Miriany A Moreira
authors.initials: MAM
authors.lastname: Fernandez
authors.affiliation: Departamento de Análises Clínicas e Toxicológicas, Universidade Federal de Minas Gerais, Av. Antônio Carlos, Pampulha 6627-Belo Horizonte/MG, 31270901, Brazil.
authors.forename: Leiliane Coelho
authors.initials: LC
authors.lastname: André
authors.affiliation: Departamento de Química, Universidade Federal de Minas Gerais, Av. Antônio Carlos, 6627-Belo Horizonte/MG, 31270901, Brazil. Electronic address: zenilda@ufmg.br.
authors.forename: Zenilda de Lourdes
authors.initials: ZL
authors.lastname: Cardeal
mesh_terms: D000293:Adolescent; D000328:Adult; D001559:Benzhydryl Compounds / Q000032:analysis* / Q000652:urine; D003404:Creatinine / Q000652:urine; D004785:Environmental Pollutants / Q000032:analysis; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D006801:Humans; D059627:Liquid Phase Microextraction / Q000379:methods*; D055442:Metabolome; D008875:Middle Aged; D010636:Phenols / Q000032:analysis* / Q000652:urine; D010968:Plasticizers / Q000032:analysis*; D012997:Solvents; D055815:Young Adult; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D003404:Creatinine; D004785:Environmental Pollutants; D010636:Phenols; D010968:Plasticizers; D012997:Solvents; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: Bisphenol A; Gas chromatography-mass spectrometry; Hollow fiber liquid phase microextraction; Phthalate metabolites; Urine samples