title: Scout-MRM: Multiplexed Targeted Mass Spectrometry-Based Assay without Retention Time Scheduling Exemplified by Dickeya dadantii Proteomic Analysis during Plant Infection.
abstract: Targeted mass spectrometry of a surrogate peptide panel is a powerful method to study the dynamics of protein networks, but chromatographic time scheduling remains a major limitation for dissemination and implementation of robust and large multiplexed assays. We unveil a Multiple Reaction Monitoring method (Scout-MRM) where the use of spiked scout peptides triggers complex transition lists, regardless of the retention time of targeted surrogate peptides. The interest of Scout-MRM method regarding the retention time independency, multiplexing capability, reproducibility, and putative interest in facilitating method transfer was illustrated by a 782-peptide-plex relative assay targeting 445 proteins of the phytopathogen Dickeya dadantii during plant infection.
authors.affiliation: Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Blandine
authors.initials: B
authors.lastname: Rougemont
authors.affiliation: Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Bontemps Gallo
authors.affiliation: Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Sophie
authors.identifier: 0000-0003-4813-5900
authors.initials: S
authors.lastname: Ayciriex
authors.affiliation: Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Romain
authors.initials: R
authors.lastname: Carrière
authors.affiliation: School of Biomedical Sciences and Pharmacy and Hunter Medical Research Institute, University of Newcastle , Callaghan, NSW 2308, Australia.
authors.forename: Hubert
authors.initials: H
authors.lastname: Hondermarck
authors.affiliation: Université de Lille, CNRS, UMR 8576 , Unité de Glycobiologie Structurale et Fonctionnelle, F 59000 Lille, France.
authors.forename: Jean Marie
authors.initials: JM
authors.lastname: Lacroix
authors.affiliation: SCIEX, 71 Four Valley Drive, Concord, Ontario L4K 4 V8, Canada.
authors.forename: J C Yves
authors.initials: JC
authors.lastname: Le Blanc
authors.affiliation: Université de Lyon, Université Claude Bernard Lyon 1, Ecole Normale Supérieure de Lyon, Institut des Sciences Analytiques , CNRS UMR 5280, 5 rue de la Doua, F-69100 Villeurbanne, France.
authors.forename: Jérôme
authors.identifier: 0000-0002-5497-6880
authors.initials: J
authors.lastname: Lemoine
mesh_terms: D018651:Cichorium intybus / Q000378:metabolism* / Q000382:microbiology; D002851:Chromatography, High Pressure Liquid; D004755:Enterobacteriaceae / Q000472:pathogenicity*; D013058:Mass Spectrometry; D010455:Peptides / Q000032:analysis*; D018515:Plant Leaves / Q000378:metabolism / Q000382:microbiology; D040901:Proteomics / Q000379:methods*
chemical_list: D010455:Peptides