title: Common SNP rs6564851 in the BCO1 Gene Affects the Circulating Levels of β-Carotene and the Daily Intake of Carotenoids in Healthy Japanese Women.
abstract: The circulating levels of β-carotene are modulated not only by sex, but also by autosomal gene variations and fruit intake. The aim of this study was to investigate the interactions between β-carotene metabolism-related gene single nucleotide polymorphisms (SNPs; genetic factors) and nutrient intake (environmental factors) relating to their effects on circulating β-carotene. The serum concentrations of β-carotene and the habitual food intake of 92 healthy Japanese adults were examined. All subjects were genotyped for three common SNPs: rs6564851 in the β-carotene 15,15'-oxygenase 1 (BCO1) gene, rs2278986 in the scavenger receptor class B member 1 (SCARB1) gene and rs362090 in the intestine-specific homeobox (ISX) gene. Univariate analysis revealed that the circulating β-carotene levels were significantly higher in rs6564851 GG homozygotes (p = 0.003). Additionally, the daily intake of β-cryptoxanthin was positively associated with the circulating β-carotene levels in female GG homozygotes of rs6564851 (p = 0.023), and the daily intake of α- and β-carotenes, and β-cryptoxanthin was significantly lower in female rs6564851 T allele carries than in female GG homozygotes (p = 0.009, 0.008, 0.009, respectively). The present study apparently indicates that higher circulating β-carotene levels in female rs6564851 GG homozygotes depend on carotenoid intake.
authors.affiliation: Molecular and Cellular Biology, Graduate School of Human Health Science, University of Nagasaki, Nagasaki, Japan.
authors.forename: Suemi
authors.initials: S
authors.lastname: Yabuta
authors.affiliation: Molecular and Cellular Biology, Graduate School of Human Health Science, University of Nagasaki, Nagasaki, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Urata
authors.affiliation: Nutritional Epidemiology, Graduate School of Human Health Science, University of Nagasaki, Nagasaki, Japan.
authors.forename: Roseline Yap
authors.initials: RY
authors.lastname: Wai Kun
authors.affiliation: Nutritional Epidemiology, Graduate School of Human Health Science, University of Nagasaki, Nagasaki, Japan.
authors.forename: Motofumi
authors.initials: M
authors.lastname: Masaki
authors.affiliation: Molecular and Cellular Biology, Graduate School of Human Health Science, University of Nagasaki, Nagasaki, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Shidoji
mesh_terms: D002338:Carotenoids / Q000097:blood* / Q000378:metabolism; D004032:Diet; D004435:Eating; D005260:Female; D005638:Fruit; D005787:Gene Frequency; D005838:Genotype; D018398:Homeodomain Proteins / Q000235:genetics; D006801:Humans; D007564:Japan; D020641:Polymorphism, Single Nucleotide; D014157:Transcription Factors / Q000235:genetics; D019207:beta Carotene / Q000097:blood* / Q000378:metabolism; D050559:beta-Carotene 15,15'-Monooxygenase / Q000235:genetics
chemical_list: D018398:Homeodomain Proteins; C579132:Isx protein, human; D014157:Transcription Factors; D019207:beta Carotene; D002338:Carotenoids; C493358:BCO1 protein, human; D050559:beta-Carotene 15,15'-Monooxygenase