title: Selenite modulates the level of phenolics and nutrient element to alleviate the toxicity of arsenite in rice (Oryza sativa L.).
abstract: Arsenic (As) contamination of paddy rice is a serious threat all over the world particularly in South East Asia. Selenium (Se) plays important role in protection of plants against various abiotic stresses including heavy metals. Moreover, arsenite (AsIII) and selenite (SeIV) can be biologically antagonistic due to similar electronic configuration and sharing the common transporter for their uptake in plant. In the present study, the response of oxidative stress, phenolic compounds and nutrient elements was analyzed to investigate Se mediated As tolerance in rice seedlings during AsIII and SeIV exposure in hydroponics. Selenite (25µM) significantly decreased As accumulation in plant than As (25µM) alone treated plants. Level of oxidative stress related parameters viz., reactive oxygen species (ROS), lipid peroxidation, electrical conductivity, nitric oxide and pro-oxidant enzyme (NADPH oxidase), were in the order of As>As+Se>control>Se. Selenium ameliorated As phytotoxicity by increased level of phenolic compounds particularly gallic acid, protocatechuic acid, ferulic acid and rutin and thiol metabolism related enzymes viz., serine acetyl transferase (SAT) and cysteine synthase (CS). Selenium supplementation enhanced the uptake of nutrient elements viz., Fe, Mn, Co, Cu, Zn, Mo, and improved plant growth. The results concluded that Se addition in As contaminated environment might be an important strategy to reduce As uptake and associated phytotoxicity in rice plant by modulation of phenolic compounds and increased uptake of nutrient elements.
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India; Department of Biotechnology, Kumaun University, Bhimtal, Nainital, Uttarakhand.
authors.forename: Reshu
authors.initials: R
authors.lastname: Chauhan
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India.
authors.forename: Surabhi
authors.initials: S
authors.lastname: Awasthi
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Tripathi
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India.
authors.forename: Seema
authors.initials: S
authors.lastname: Mishra
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India.
authors.forename: Sanjay
authors.initials: S
authors.lastname: Dwivedi
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Niranjan
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India.
authors.forename: Shekhar
authors.initials: S
authors.lastname: Mallick
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India.
authors.forename: Pratibha
authors.initials: P
authors.lastname: Tripathi
authors.affiliation: Department of Biotechnology, Kumaun University, Bhimtal, Nainital, Uttarakhand.
authors.forename: Veena
authors.initials: V
authors.lastname: Pande
authors.affiliation: C.S.I.R.-National Botanical Research Institute, Council of Scientific and Industrial Research, Lucknow 226001, India. Electronic address: tripathird@gmail.com.
authors.forename: Rudra Deo
authors.initials: RD
authors.lastname: Tripathi
mesh_terms: D001151:Arsenic / Q000378:metabolism / Q000633:toxicity*; D018053:Arsenites / Q000633:toxicity; D015227:Lipid Peroxidation / Q000187:drug effects; D019216:Metals, Heavy / Q000378:metabolism; D019255:NADPH Oxidases / Q000378:metabolism; D012275:Oryza / Q000187:drug effects* / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000378:metabolism; D018517:Plant Roots / Q000378:metabolism; D017382:Reactive Oxygen Species / Q000378:metabolism; D036226:Seedlings / Q000378:metabolism; D018038:Sodium Selenite / Q000494:pharmacology*; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013723:Teratogens / Q000633:toxicity*; D014874:Water Pollutants, Chemical / Q000378:metabolism / Q000633:toxicity*
chemical_list: D018053:Arsenites; D019216:Metals, Heavy; D010636:Phenols; D017382:Reactive Oxygen Species; D013438:Sulfhydryl Compounds; D013723:Teratogens; D014874:Water Pollutants, Chemical; D019255:NADPH Oxidases; D018038:Sodium Selenite; D001151:Arsenic
keywords: Arsenic; Nutrient elements; Phenolics; ROS staining; Selenite