title: Emphysema induced by elastase enhances acute inflammatory pulmonary response to intraperitoneal LPS in rats.
abstract: Abnormalities in lungs caused by emphysema might alter their response to sepsis and the occurrence of acute lung injury (ALI). This study compared the extension of ALI in response to intraperitoneal lipopolysaccharide (LPS) injection in Wistar rats with and without emphysema induced by elastase. Adult male Wistar rats were randomized into four groups: control, emphysema without sepsis, normal lung with sepsis and emphysema with sepsis. Sepsis was induced, and 24 h later the rats were euthanised. The following analysis was performed: blood gas measurements, bronchoalveolar lavage (BAL), lung permeability and histology. Animals that received LPS showed significant increase in a lung injury scoring system, inflammatory cells in bronchoalveolar lavage (BAL) and IL-6, TNF-α and CXCL2 mRNA expression in lung tissue. Animals with emphysema and sepsis showed increased alveolocapillary membrane permeability, demonstrated by higher BAL/serum albumin ratio. In conclusion, the presence of emphysema induced by elastase increases the inflammatory response in the lungs to a systemic stimulus, represented in this model by the intraperitoneal injection of LPS.
authors.affiliation: Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.|Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
authors.forename: Lídia Maria Carneiro
authors.identifier: 0000-0002-9624-2359
authors.initials: LM
authors.lastname: da Fonseca
authors.affiliation: Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.|Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
authors.forename: Maycon Moura
authors.initials: MM
authors.lastname: Reboredo
authors.affiliation: Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.|Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.|Department of Morphology, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
authors.forename: Leda Marília Fonseca
authors.initials: LM
authors.lastname: Lucinda
authors.affiliation: Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.|Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
authors.forename: Thaís Fernanda
authors.initials: TF
authors.lastname: Fazza
authors.affiliation: Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.|Department of Morphology, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
authors.forename: Maria Aparecida Esteves
authors.initials: MA
authors.lastname: Rabelo
authors.affiliation: Department of Biophysics and Biometry, Institute of Biology Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Juiz de Fora, Brazil.
authors.forename: Adenilson Souza
authors.initials: AS
authors.lastname: Fonseca
authors.affiliation: Department of Morphology, Institute of Biological Sciences, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
authors.forename: Flavia
authors.initials: F
authors.lastname: de Paoli
authors.affiliation: Pulmonary Research Laboratory, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.|Center of Reproductive Biology, Universidade Federal de Juiz de Fora, Juiz de Fora, Brazil.
authors.forename: Bruno Valle
authors.initials: BV
authors.lastname: Pinheiro
mesh_terms: D055371:Acute Lung Injury / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid; D054426:Chemokine CXCL2 / Q000235:genetics / Q000378:metabolism; D004195:Disease Models, Animal; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D007274:Injections, Intraperitoneal; D015850:Interleukin-6 / Q000235:genetics / Q000378:metabolism; D008070:Lipopolysaccharides / Q000009:adverse effects; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008297:Male; D010196:Pancreatic Elastase / Q000009:adverse effects*; D011656:Pulmonary Emphysema / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017208:Rats, Wistar; D012128:Respiratory Distress Syndrome / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D018805:Sepsis / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics / Q000378:metabolism
chemical_list: C499706:CXCL2 protein, human; D054426:Chemokine CXCL2; D015850:Interleukin-6; D008070:Lipopolysaccharides; D014409:Tumor Necrosis Factor-alpha; D010196:Pancreatic Elastase
keywords: acute lung injury; acute respiratory distress syndrome; lipopolysaccharides; pancreatic elastase; pulmonary emphysema; sepsis