title: Murine monoclonal antibodies directed against human recombinant Macrophage Migration Inhibitory Factor.
abstract: Macrophage Migration Inhibitory Factor (MIF) is a crucial component of the immune system acting together with glucocorticosteroids to regulate immunity and inflammation. Understanding of its many putative functions and action mechanisms is still ambiguous. Due to the newest findings that a local MIF expression is up regulated in allograft rejection and in glomerulonephritis, an interest in MIF research is increasing and is focused on possibilities of anti-MIF treatment.In the present work new murine monoclonal antibodies (MAbs) directed against human recombinant MIF (hrMIF) are described. hrMIF protein used for the immunisation was tested for its biological activity and has evident macrophage migration inhibitory activity. The selected MAbs were purified and further characterised. They recognised MIF in a Western blot experiment after a native IEF. Anti-MIF MAb designated as Ml inhibited MIF activity in the test, which was performed in the 48 well Boyden chamber system. It is presumed that Ml MAb could be used as a potential therapeutic agent.
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Ruth Rebeka
authors.initials: RR
authors.lastname: Rupreht
authors.affiliation: BIOHIT, d.o.o., Gorazdova 7, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Bojana
authors.initials: B
authors.lastname: Mozetič-Francky
authors.affiliation: BIOHIT, d.o.o., Gorazdova 7, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Andrej
authors.initials: A
authors.lastname: Francky
authors.affiliation: Institute of Microbiology and Immunology, Korytkova 2, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Maja
authors.initials: M
authors.lastname: Matis
authors.affiliation: Institute of Microbiology and Immunology, Korytkova 2, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Mojca
authors.initials: M
authors.lastname: Škoberne
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Galvani
authors.affiliation: Institute of Microbiology and Immunology, Korytkova 2, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Tadej
authors.initials: T
authors.lastname: Malovrh
authors.affiliation: Institute of Microbiology and Immunology, Korytkova 2, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Vladimir
authors.initials: V
authors.lastname: Kotnik
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Vladka Čurin
authors.initials: VČ
authors.lastname: Šerbec
keywords: Key words MIF; anti-MIF MAb; anti-MIF therapy; immune response