title: Rapid and sensitive detection of bisphenol a from serum matrix.
abstract: Bisphenol A (BPA) is an endocrine disrupting compound that may have adverse developmental, reproductive, neurological, and immune system effects. Low-level exposure to BPA is ubiquitous in human populations due to its widespread use in consumer products. Therefore, highly sensitive methods are needed to quantify BPA in various matrices including water, serum, and food products. In this study, we developed a simple, rapid, highly sensitive and specific sensor based on an aptamer probe and AC electrokinetics capacitive sensing method that successfully detected BPA at femto molar (fM) levels, which is an improvement over prior work by a factor of 10. We were able to detect BPA spiked in human serum as well as in maternal and cord blood within 30s. The sensor is responsive to BPA down to femto molar levels, but not to structurally similar compounds including bisphenol F (BPF) or bisphenol S (BPS) even at much higher concentration. Further development of this platform may prove useful in monitoring exposure to BPA and other small molecules in various matrices.
authors.affiliation: Key Laboratory of Optoelectronic Technology and System of the Education Ministry of China, Chongqing University, Chongqing 400044, China; Department of Electrical Engineering and Computer Science, the University of Tennessee, Knoxville, TN 37996, USA.
authors.forename: Xiaogang
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Electrical Engineering and Computer Science, the University of Tennessee, Knoxville, TN 37996, USA.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cheng
authors.affiliation: Department of Medicine, Graduate School of Medicine, the University of Tennessee Medical Center, USA.
authors.forename: Paul
authors.initials: P
authors.lastname: Terry
authors.affiliation: Department of Public Health, the University of Tennessee, Knoxville, TN 37996, USA. Electronic address: jchen38@utk.edu.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Electrical Engineering and Computer Science, the University of Tennessee, Knoxville, TN 37996, USA.
authors.forename: Haochen
authors.initials: H
authors.lastname: Cui
authors.affiliation: Department of Electrical Engineering and Computer Science, the University of Tennessee, Knoxville, TN 37996, USA. Electronic address: jwu10@utk.edu.
authors.forename: Jayne
authors.initials: J
authors.lastname: Wu
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D001559:Benzhydryl Compounds / Q000097:blood*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D025802:Electric Capacitance; D055664:Electrochemical Techniques / Q000295:instrumentation; D052244:Endocrine Disruptors / Q000097:blood*; D004867:Equipment Design; D005260:Female; D005312:Fetal Blood / Q000737:chemistry; D006801:Humans; D057230:Limit of Detection; D008839:Microelectrodes; D010636:Phenols / Q000097:blood*; D011247:Pregnancy; D013997:Time Factors; D000098826:Bisphenol A Compounds
chemical_list: D052157:Aptamers, Nucleotide; D001559:Benzhydryl Compounds; D052244:Endocrine Disruptors; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: AC electrokinetics; Bisphenol A, Affinity sensor; Capacitive biosensor