title: Synergistic effect of olfactory ensheathing cells and alpha-crystallin on restoration of adult rat optic nerve injury.
abstract: Olfactory enshesathing cells (OECs) and α-crystallin all can promote axonal regeneration after optic nerve injury. However, their mechanisms were different. Here, we study the synergistic effect of OECs and α-crystallin on the optic nerve regeneration. α-Crystallin was injected into vitreous cavity, and OECs were transplanted to the optic nerve injury area. The regeneration length of optic nerve were measured by anterograde tracing using cholera toxin subunit B (CTB). The survival of RGCs were assessed by counting the numbers of βIII-tubulin-labeled RGCs in a retinal whole mount. The results that OECs and α-crystallin all could promoted RGCs survival and axonal regeneration (P<0.01). Especially in the combination group, the length of axonal regeneration was 5.6mm after optic nerve injury for 3 months. These findings indicated that compared to OECs and α-crystallin alone, the combination of OECs and α-crystallin could promote axonal regeneration more effectively.
authors.affiliation: Department of Ophthalmology, General Hospital of Chinese People's Liberation Army, Beijing 100853, PR China; Taiyuan Aier Eye Hospital, Aier Eye Hospital Group, Taiyuan 030000, PR China.
authors.forename: Yan Hua
authors.initials: YH
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, General Hospital of Chinese People's Liberation Army, Beijing 100853, PR China; Southwest Hospital, Southwest Eye Hospital, Third Military Medical University, Chongqing 400038, PR China. Electronic address: yzhengqin@163.com.
authors.forename: Zheng Qin
authors.initials: ZQ
authors.lastname: Yin
authors.affiliation: Southwest Hospital, Southwest Eye Hospital, Third Military Medical University, Chongqing 400038, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D001369:Axons / Q000502:physiology; D002470:Cell Survival; D002478:Cells, Cultured; D005347:Fibroblasts / Q000637:transplantation; D009409:Nerve Crush; D009416:Nerve Regeneration; D009457:Neuroglia / Q000637:transplantation*; D009830:Olfactory Bulb / Q000166:cytology*; D020221:Optic Nerve Injuries / Q000473:pathology / Q000503:physiopathology / Q000628:therapy*; D020318:Rats, Long-Evans; D012165:Retinal Ganglion Cells / Q000473:pathology; D038201:alpha-Crystallins / Q000627:therapeutic use*
chemical_list: D038201:alpha-Crystallins
keywords: Axonal regeneration; Olfactory ensheathing cells; α-crystallin