title: [Development of blood-brain barrier under the modulation of HIF activity in astroglialand neuronal cells in vitro].
abstract: Barriergenesis is the process of maturation of the primary vascular network of the brain responsible for the establishment of the blood-brain barrier. It represents a combination of factors that, on the one hand, contribute to the process of migration and tubulogenesis of endothelial cells (angiogenesis), on the other hand, contribute to the formation of new connections between endothelial cells and other elements of the neurovascular unit. Astrocytes play a key role in barriergenesis, however, mechanisms of their action are still poorly examined. We have studied the effects of HIF-1 modulators acting on the cells of non-endothelial origin (neurons and astrocytes) on the development of the blood-brain barrier in vitro. Application of FM19G11 regulating expression of HIF-1 activity and GSI-1 suppressing gamma-secretase and/or proteasomal activity resulted in the elevated expression of thrombospondins and matrix metalloproteinases in the developing blood-brain barrier. However, it caused the opposite effect on VEGF expression thus promoting barrier maturation in vitro.
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: V A
authors.initials: VA
authors.lastname: Ruzaeva
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Morgun
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: E D
authors.initials: ED
authors.lastname: Khilazheva
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Kuvacheva
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Pozhilenkova
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: E B
authors.initials: EB
authors.lastname: Boitsova
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: G P
authors.initials: GP
authors.lastname: Martynova
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: T E
authors.initials: TE
authors.lastname: Taranushenko
authors.affiliation: Voino-Yasenetsky Krasnoyarsk State Medical University, Krasnoyarsk, Russia.
authors.forename: A B
authors.initials: AB
authors.lastname: Salmina
mesh_terms: D000818:Animals; D001253:Astrocytes / Q000166:cytology / Q000378:metabolism*; D001549:Benzamides / Q000494:pharmacology; D001812:Blood-Brain Barrier / Q000166:cytology / Q000378:metabolism*; D002478:Cells, Cultured; D017364:Collagenases / Q000378:metabolism; D051793:Hypoxia-Inducible Factor 1 / Q000378:metabolism*; D009474:Neurons / Q000166:cytology / Q000378:metabolism*; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar
chemical_list: D001549:Benzamides; C555528:FM19G11; D051793:Hypoxia-Inducible Factor 1; D017364:Collagenases; D046988:Proteasome Endopeptidase Complex
keywords: HIF-1 modulators; angiogenesis; barriergenesis; blood-brain barrier; neurovascular unit