title: Nervous necrosis virus capsid protein exploits nucleolar phosphoprotein Nucleophosmin (B23) function for viral replication.
abstract: Nucleolar proteins facilitate the replication of certain human and animal viruses through interaction with viral proteins. In this study, an interaction between nervous necrosis virus capsid protein and nucleolar phosphoprotein B23 was identified using in vitro experimental approaches. The capsid protein binds to B23 early during the viral infection and accumulates in the nucleus, particularly in the nucleolus. However, over the course of the infection B23 is redistributed from the nucleoli to the nucleoplasm. siRNA-mediated knockdown of B23 reduced viral replication and cytopathic effect. Thus, B23 targets capsid protein to the nucleus and facilitates NNV replication. The results provide the first demonstration that nucleolar protein B23 has a direct role in the nodavirus replication process.
authors.affiliation: The Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China. Electronic address: mwj9876@mail.ujs.edu.cn.
authors.forename: Weijun
authors.initials: W
authors.lastname: Mai
authors.affiliation: The Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Huang
authors.affiliation: The Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Huiqing
authors.initials: H
authors.lastname: Chen
authors.affiliation: The Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: The Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D036022:Capsid Proteins / Q000235:genetics* / Q000378:metabolism; D002466:Cell Nucleolus / Q000378:metabolism / Q000648:ultrastructure / Q000821:virology; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D029941:Fish Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D005786:Gene Expression Regulation; D005822:Genetic Vectors / Q000737:chemistry / Q000378:metabolism; D054884:Host-Pathogen Interactions; D030381:Nodaviridae / Q000235:genetics* / Q000378:metabolism; D009687:Nuclear Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D000090243:Nucleophosmin; D010473:Perciformes / Q000821:virology; D010750:Phosphoproteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D011485:Protein Binding; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D014779:Virus Replication
chemical_list: D036022:Capsid Proteins; D029941:Fish Proteins; C000716967:NPM1 protein, human; D009687:Nuclear Proteins; D010750:Phosphoproteins; D034741:RNA, Small Interfering; D011994:Recombinant Proteins; D000090243:Nucleophosmin
keywords: Capsid protein; Interaction; Nervous necrosis virus; Nucleolar protein B23; Viral replication