title: Immunological Roles of Elevated Plasma Levels of Matricellular Proteins in Japanese Patients with Pulmonary Tuberculosis.
abstract: Elevated matricellular proteins (MCPs), including osteopontin (OPN) and galectin-9 (Gal-9), were observed in the plasma of patients with Manila-type tuberculosis (TB) previously. Here, we quantified plasma OPN, Gal-9, and soluble CD44 (sCD44) by enzyme-linked immunosorbent assay (ELISA), and another 29 cytokines by Luminex assay in 36 patients with pulmonary TB, six subjects with latent tuberculosis (LTBI), and 19 healthy controls (HCs) from Japan for a better understanding of the roles of MCPs in TB. All TB subjects showed positive results of enzyme-linked immunospot assays (ELISPOTs). Spoligotyping showed that 20 out of 36Mycobacterium tuberculosis(MTB) strains belong to the Beijing type. The levels of OPN, Gal-9, and sCD44 were higher in TB (positivity of 61.1%, 66.7%, and 63.9%, respectively) than in the HCs. Positive correlations between OPN and Gal-9, between OPN and sCD44, and negative correlation between OPN and ESAT-6-ELISPOT response, between chest X-ray severity score of cavitary TB and ESAT-6-ELISPOT response were observed. Instead of OPN, Gal-9, and sCD44, cytokines G-CSF, GM-CSF, IFN-α, IFN-γ, IL-12p70, and IL-1RA levels were higher in Beijing MTB-infected patients. These findings suggest immunoregulatory, rather than inflammatory, effect of MCPs and can advance the understanding of the roles of MCPs in the context of TB pathology.
authors.affiliation: Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan. beatabucekova@gmail.com.
authors.forename: Beata
authors.initials: B
authors.lastname: Shiratori
authors.affiliation: Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan. zhaojingge1987@gmail.com.
authors.forename: Jingge
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Respiratory Medicine, Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. okumuram@fukujuji.org.
authors.forename: Masao
authors.initials: M
authors.lastname: Okumura
authors.affiliation: Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan. haorile@gmail.com.
authors.forename: Haorile
authors.initials: H
authors.lastname: Chagan-Yasutan
authors.affiliation: Department of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-2 4 Matsuyama, Kiyose, Tokyo 204-8533, Japan. yanaih@fukujuji.org.
authors.forename: Hideki
authors.initials: H
authors.lastname: Yanai
authors.affiliation: Department of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-2 4 Matsuyama, Kiyose, Tokyo 204-8533, Japan. hiralin99@yahoo.co.jp.
authors.forename: Kazue
authors.initials: K
authors.lastname: Mizuno
authors.affiliation: Department of Respiratory Medicine, Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan. yoshiyamat@fukujuji.org.
authors.forename: Takashi
authors.initials: T
authors.lastname: Yoshiyama
authors.affiliation: Department of Clinical Laboratory, Fukujuji Hospital, Japan Anti-Tuberculosis Association, 3-1-2 4 Matsuyama, Kiyose, Tokyo 204-8533, Japan. ideit@fukujuji.org.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Idei
authors.affiliation: Division of Disaster-Related Infectious Diseases, International Research Institute of Disaster Science, Tohoku University, 2-1 Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8575, Japan. ya82@yahoo.co.jp.
authors.forename: Yugo
authors.initials: Y
authors.lastname: Ashino
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan. cnakajim@czc.hokudai.ac.jp.
authors.forename: Chie
authors.initials: C
authors.lastname: Nakajima
authors.affiliation: Division of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, North 20, West 10, Kita-ku, Sapporo, Hokkaido 001-0020, Japan. suzuki@czc.hokudai.ac.jp.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Health Science and Social Welfare, Kibi International University, 8 Igamachi, Takahashi 716-8508, Japan. hattorit@kiui.ac.jp.
authors.forename: Toshio
authors.initials: T
authors.lastname: Hattori
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D037161:Galectins / Q000097:blood*; D006801:Humans; D018960:Hyaluronan Receptors / Q000097:blood*; D007564:Japan; D008297:Male; D008875:Middle Aged; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics / Q000302:isolation & purification; D053495:Osteopontin / Q000097:blood*; D014397:Tuberculosis, Pulmonary / Q000097:blood* / Q000276:immunology / Q000382:microbiology
chemical_list: D037161:Galectins; D018960:Hyaluronan Receptors; D053495:Osteopontin
keywords: Beijing genotype MTB; CD44; galectin-9; osteopontin; tuberculosis