title: IL17-Producing γδ T Cells May Enhance Humoral Immunity during PulmonaryPseudomonas aeruginosaInfection in Mice.
abstract: The host acquired immune response, especially the humoral immunity, plays key roles in preventing bacterial pneumonia in the lung. Our previous research demonstrated that interleukin 17-producing γδ T cells (IL17-γδ T cells) have a protective effect on the early innate immune response during acute pulmonaryPseudomonas aeruginosainfection. However, whether IL17-γδ T cells also play a role in humoral immunity is unknown. In this study, an acute pulmonaryP. aeruginosainfection model was established in wild-type and γδ TCR-/-C57BL/6 mice. The expression of IL-17 on γδ T cells isolated from infected lung tissues increased rapidly and peaked at day 7 after acute infection withP. aeruginosa. Compared with wild-type infected mice, the levels of total immunoglobulins including IgA, IgG, and IgM in the serum and BALF were significantly decreased in γδ TCR-/-mice, with the exception of IgM in the BALF. Moreover, CD69 expression in B cells from the lungs and spleen and the level of BAFF in the plasma were also decreased in γδ TCR-/-mice. IL17-γδ T cell transfusion significantly improved the production of immunoglobulins, B cell activation and BAFF levels in γδ TCR-/-mice compared with γδ TCR-/-mice without transfusion; this effect was blocked when cells were pretreated with an IL-17 antibody. Together, these data demonstrate that IL17-γδ T cells are involved in CD19+B cell activation and the production of immunoglobulins during acute pulmonaryP. aeruginosainfection. Thus, we conclude that IL17-γδ T cells may facilitate the elimination of bacteria and improve survival through not only innate immunity but also humoral immunity.
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Pan
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Ruoming
authors.initials: R
authors.lastname: Tan
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Meiling
authors.initials: M
authors.lastname: Li
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Zhaojun
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Critical Care Medicine, Ruijin Hospital, Shanghai Jiaotong University School of Medicine Shanghai, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Qu
mesh_terms: D000818:Animals; D000907:Antibodies, Bacterial / Q000097:blood*; D015703:Antigens, CD / Q000032:analysis; D000945:Antigens, Differentiation, T-Lymphocyte / Q000032:analysis; D053264:B-Cell Activating Factor / Q000097:blood; D016175:B-Lymphocyte Subsets / Q000737:chemistry / Q000276:immunology*; D001774:Blood Chemical Analysis; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D007070:Immunoglobulin A / Q000032:analysis / Q000097:blood; D007074:Immunoglobulin G / Q000032:analysis / Q000097:blood; D007075:Immunoglobulin M / Q000032:analysis / Q000097:blood; D020381:Interleukin-17 / Q000378:metabolism; D037181:Lectins, C-Type / Q000032:analysis; D008168:Lung / Q000276:immunology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D018410:Pneumonia, Bacterial / Q000276:immunology*; D011552:Pseudomonas Infections / Q000276:immunology*; D011550:Pseudomonas aeruginosa / Q000276:immunology*; D013154:Spleen / Q000276:immunology; D016176:T-Lymphocyte Subsets / Q000276:immunology*; D000099192:CD69 Antigens
chemical_list: D000907:Antibodies, Bacterial; D015703:Antigens, CD; D000945:Antigens, Differentiation, T-Lymphocyte; D053264:B-Cell Activating Factor; D007070:Immunoglobulin A; D007074:Immunoglobulin G; D007075:Immunoglobulin M; D020381:Interleukin-17; D037181:Lectins, C-Type; D000099192:CD69 Antigens; C505720:Tnfsf13b protein, mouse
keywords: B cell activating factor; B cells; IL17-γδ T cells; immunoglobulin(s)