title: Electrospun polyurethane membranes for Tissue Engineering applications.
abstract: Tissue Engineering proposes, among other things, tissue regeneration using scaffolds integrated with biological molecules, growth factors or cells for such regeneration. In this research, polyurethane membranes were prepared using the electrospinning technique in order to obtain membranes to be applied in Tissue Engineering, such as epithelial, drug delivery or cardiac applications. The influence of fibers on the structure and morphology of the membranes was studied using scanning electron microscopy (SEM), the structure was evaluated by Fourier transform infrared spectroscopy (FT-IR), and the thermal stability was analyzed by thermogravimetry analysis (TGA). In vitro cells attachment and proliferation was investigated by SEM, and in vitro cell viability was studied by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assays and Live/Dead® assays. It was found that the membranes present an homogeneous morphology, high porosity, high surface area/volume ratio, it was also observed a random fiber network. The thermal analysis showed that the membrane degradation started at 254°C. In vitro evaluation of fibroblasts cells showed that fibroblasts spread over the membrane surface after 24, 48 and 72h of culture. This study supports the investigation of electrospun polyurethane membranes as biocompatible scaffolds for Tissue Engineering applications and provides some guidelines for improved biomaterials with desired properties.
authors.affiliation: National Institute of Biofabrication, Campinas, Brazil; Department of Chemical Engineering, University of Campinas, Campinas, Brazil. Electronic address: lagabriel@gmail.com.
authors.forename: Laís P
authors.initials: LP
authors.lastname: Gabriel
authors.affiliation: National Institute of Biofabrication, Campinas, Brazil; Department of Medical Sciences, University of Campinas, Campinas, Brazil.
authors.forename: Ana Amélia
authors.initials: AA
authors.lastname: Rodrigues
authors.affiliation: National Institute of Biofabrication, Campinas, Brazil; Department of Chemical Engineering, University of Campinas, Campinas, Brazil.
authors.forename: Milton
authors.initials: M
authors.lastname: Macedo
authors.affiliation: National Institute of Biofabrication, Campinas, Brazil; Department of Chemical Engineering, University of Campinas, Campinas, Brazil.
authors.forename: André L
authors.initials: AL
authors.lastname: Jardini
authors.affiliation: National Institute of Biofabrication, Campinas, Brazil; Department of Chemical Engineering, University of Campinas, Campinas, Brazil.
authors.forename: Rubens
authors.initials: R
authors.lastname: Maciel Filho
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D002522:Chlorocebus aethiops; D008855:Microscopy, Electron, Scanning; D011140:Polyurethanes / Q000737:chemistry*; D016062:Porosity; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D013818:Thermogravimetry; D023822:Tissue Engineering; D054457:Tissue Scaffolds; D014709:Vero Cells
chemical_list: D001672:Biocompatible Materials; D011140:Polyurethanes
keywords: Electrospinning; Fibroblast proliferation; Implants; Polyurethane