title: Circulating miRNAs as biomarkers for oral squamous cell carcinoma recurrence in operated patients.
abstract: MicroRNAs (miRNAs) are small regulatory non-coding RNAs for which altered expression in cancers can serve as potential biomarkers for diseases. We here investigated whether circulating miRNAs can serve as biomarkers for predicting post-operational recurrence of oral squamous cell carcinoma (OSCC) in patients. Plasma samples from 8 Danish OSCC patients were collected before, and one year after surgical operation, as well as from 3 Danish healthy controls and subjected to miRNA profiling by next generation sequencing. Disease recurrence did not occur in the 8 patients when the post-operative plasma samples were collected. Based on the sequencing data, three up-regulated miRNAs (miR-148a-3p, miR-26a-5p and miR-21-5p) and three down-regulated miRNAs (miR-375, miR-92b-3p and miR-486-5p) in the OSCC samples compared to healthy controls were selected for qRT-PCR validation in a Chinese cohort of 20 plasma samples collected before, and 9-12 months after surgical operation, and 18 healthy controls. Disease recurrence had occurred in 8 out of the 20 Chinese patients at the time their post-operative plasma samples were collected. The results of qRT-PCR showed that down-regulation of miR-486-5p, miR-375 and miR-92b-3p were highly associated with OSCC recurrence. This study indicates that the plasma miRNA profile is altered in OSCC during its progression and can be used to monitor the likelihood of OSCC recurrence in patients after surgery.
authors.affiliation: Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Department of Oral Medicine, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.|Department of Stomatology, Beijing Tongren Hospital, Capital Medical University, Beijing, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.|Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
authors.forename: Morten T
authors.initials: MT
authors.lastname: Venø
authors.affiliation: Department of Plastic Surgery, Odense University Hospital, Odense, Denmark.
authors.forename: Vivi
authors.initials: V
authors.lastname: Bakholdt
authors.affiliation: Department of Plastic Surgery, Odense University Hospital, Odense, Denmark.
authors.forename: Jens A
authors.initials: JA
authors.lastname: Sørensen
authors.affiliation: Department of Pathology, Odense University Hospital, Odense, Denmark.
authors.forename: Annelise
authors.initials: A
authors.lastname: Krogdahl
authors.affiliation: Department of Oral Medicine, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Sun
authors.affiliation: Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.|R&D Center, Suzhou Ribo Life Science Co., Ltd, Suzhou, China.
authors.forename: Shan
authors.initials: S
authors.lastname: Gao
authors.affiliation: Interdisciplinary Nanoscience Center, Aarhus University, Aarhus, Denmark.|Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.
authors.forename: Jørgen
authors.initials: J
authors.lastname: Kjems
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D002294:Carcinoma, Squamous Cell / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000601:surgery*; D016022:Case-Control Studies; D002681:China; D000074166:Circulating MicroRNA / Q000097:blood / Q000235:genetics*; D003718:Denmark; D018572:Disease-Free Survival; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods; D006258:Head and Neck Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000601:surgery*; D059014:High-Throughput Nucleotide Sequencing; D006801:Humans; D008297:Male; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000097:blood / Q000235:genetics* / Q000473:pathology / Q000601:surgery*; D009364:Neoplasm Recurrence, Local; D016133:Polymerase Chain Reaction; D011237:Predictive Value of Tests; D012334:RNA, Neoplasm / Q000097:blood / Q000235:genetics*; D015203:Reproducibility of Results; D012307:Risk Factors; D000077195:Squamous Cell Carcinoma of Head and Neck; D013997:Time Factors; D059467:Transcriptome; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D000074166:Circulating MicroRNA; D012334:RNA, Neoplasm
keywords: NGS; OSCC; circulating miRNA; qRT–PCR; recurrence