title: Low expression of RASSF10 is associated with poor survival in patients with colorectal cancer.
abstract: The RASSF10 has been identified as a tumor suppressor in human colorectal cancer (CRC). However, the expression of RASSF10 in patients with CRC has not been evaluated for its potential use as a biomarker in the diagnosis and prognosis assessment of CRC. We analyzed the expression of RASSF10 mRNA (n=30) and protein (n=205) in CRC and matched noncancerous colon tissue samples to explore the relationships among RASSF10 expression, clinicopathological factors, and prognosis in patients with CRC. Our results showed that the expression of RASSF10 mRNA and protein in CRC-adjacent tissues was higher than that in CRC tissues. Low RASSF10 expression was associated with the T stage (P=.037, odds ratio, 0.664; 95% confidence interval, 0.452-0.975) and the N stage (P<.001, odds ratio, 0.318; 95% confidence interval, 0.184-0.549) of the tumors. In addition, univariate analysis revealed that patients with CRC with lower RASSF10 expression had poorer overall survival (OS; P<.001) and disease-free survival (DFS; P<.001). The 5-year OS and DFS rates were 48.2% and 28.3%, respectively, in patients with low RASSF10 expression and 82.2% and 62.6%, respectively, in patients with high RASSF10 expression. Multivariate Cox regression analysis revealed that the strongest predictors of OS and DFS were RASSF10 expression (P<.001 and P<.001, respectively), T stage (P=.003 and P=.009, respectively), and N stage (P=.005 and P=.026, respectively). These results demonstrate that low expression of RASSF10 in CRC tissues is significantly correlated with poor survival after curative resection and may serve as a useful biomarker predictive of CRC prognosis.
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China; Department of Gastrointestinal Surgery, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Yi-Lin
authors.initials: YL
authors.lastname: Hu
authors.affiliation: Department of Pathology, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Liu
authors.affiliation: Department of Surgical Comprehensive Laboratory, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China.
authors.forename: Yi-Bing
authors.initials: YB
authors.lastname: Guo
authors.affiliation: Department of General Surgery, Hospital of Traditional Chinese Medicine of Nantong City, Nantong, Jiangsu 226001, China.
authors.forename: Xin-Lin
authors.initials: XL
authors.lastname: Jiang
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China. Electronic address: maoqsh@sina.com.
authors.forename: Qin-Sheng
authors.initials: QS
authors.lastname: Mao
authors.affiliation: Department of General Surgery, Nantong University Affiliated Hospital, Nantong, Jiangsu 226001, China. Electronic address: 377209255@qq.com.
authors.forename: Wan-Jiang
authors.initials: WJ
authors.lastname: Xue
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000235:genetics; D001706:Biopsy; D016009:Chi-Square Distribution; D003082:Colectomy; D015179:Colorectal Neoplasms / Q000737:chemistry* / Q000235:genetics / Q000401:mortality / Q000473:pathology; D018572:Disease-Free Survival; D015536:Down-Regulation; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D053208:Kaplan-Meier Estimate; D008297:Male; D008875:Middle Aged; D015999:Multivariate Analysis; D009367:Neoplasm Staging; D016017:Odds Ratio; D016016:Proportional Hazards Models; D012333:RNA, Messenger / Q000235:genetics; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome; D025521:Tumor Suppressor Proteins / Q000032:analysis* / Q000235:genetics
chemical_list: D014408:Biomarkers, Tumor; C548440:RASSF10 protein, human; D012333:RNA, Messenger; D025521:Tumor Suppressor Proteins
keywords: CRC; Immunohistochemistry; Prognosis; RASSF10; Tumor-suppressor gene