title: Ratoon rice generated from primed parent plants exhibit enhanced herbivore resistance.
abstract: Rice ratooning is practiced in many rice-growing countries for achieving increased rice production with limited labour input. Here, we report that attack by insect herbivores, or treatment with a defense signaling compound in parent plants, can prime anti-herbivore defense responses in subsequent ratoon plants. We compared the defense responses of rice ratoons generated from parent plants that had been either infested by Cnaphalocrocis medinalis (rice leaffolder, LF) caterpillars or treated with methyl jasmonate (MeJA) during vegetative growth, with ratoons generated from control parent plants. Ratoon plants generated from parents receiving prior LF infestation or MeJA treatment exhibited higher jasmonic acid (JA) levels, as well as elevated levels of transcripts of defense-related genes associated with JA signaling. In addition, elevated activities of peroxidase, polyphenol oxidase and trypsin protease inhibitor were observed, as well as enhanced resistance towards subsequent LF infestation. Pre-priming of ratoon defense responses was significantly reduced in plants where expression of OsAOS (allene oxide synthase, involved in JA biosynthesis) or OsCOI1 (CORONATINE INSENSITIVE1, involved in JA perception) was inhibited by RNA interference. Our results indicate that herbivore exposure or MeJA treatment in rice parent plants enhances anti-herbivore resistance in subsequently generated ratoons through priming of JA-mediated defenses.
authors.affiliation: State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Wushan, Guangzhou, China.|Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China.|Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Institute of Entomology, College of Agriculture, Guizhou University, Guiyang, Guizhou, 550025, China.
authors.forename: Mao
authors.initials: M
authors.lastname: Ye
authors.affiliation: Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China.
authors.forename: Yuan-Yuan
authors.initials: YY
authors.lastname: Song
authors.affiliation: Natural Products Utilization Research Unit, Agricultural Research Service, US Department of Agriculture, University, MS, 38677, USA.
authors.forename: Scott R
authors.initials: SR
authors.lastname: Baerson
authors.affiliation: State Key Laboratory of Conservation and Utilization of Subtropical Agro-bioresources, College of Natural Resources and Environment, South China Agricultural University, Wushan, Guangzhou, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Long
authors.affiliation: Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: Natural Products Utilization Research Unit, Agricultural Research Service, US Department of Agriculture, University, MS, 38677, USA.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China.
authors.forename: Wen-Xiong
authors.initials: WX
authors.lastname: Lin
authors.affiliation: Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, China.
authors.forename: Ren-Sen
authors.identifier: http://orcid.org/0000-0002-6435-3054
authors.initials: RS
authors.lastname: Zeng
mesh_terms: D000085:Acetates / Q000494:pharmacology; D000818:Animals; D003517:Cyclopentanes / Q000378:metabolism / Q000494:pharmacology; D060467:Disease Resistance; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D060434:Herbivory / Q000502:physiology*; D007814:Larva / Q000187:drug effects / Q000502:physiology; D007915:Lepidoptera / Q000502:physiology; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000469:parasitology* / Q000502:physiology*; D054883:Oxylipins / Q000378:metabolism / Q000494:pharmacology; D010935:Plant Diseases / Q000469:parasitology*; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011480:Protease Inhibitors / Q000494:pharmacology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D000085:Acetates; D003517:Cyclopentanes; D054883:Oxylipins; D010940:Plant Proteins; D011480:Protease Inhibitors; D012333:RNA, Messenger; C011006:jasmonic acid; C072239:methyl jasmonate
keywords: JA signaling pathway; anti-herbivore resistance; defense priming; leaffolder; ratoon rice; rice (Oryza sativa)