title: A probiotic combination attenuates experimental colitis through inhibition of innate cytokine production.
abstract: Inflammatory bowel disease (IBD) is a severe immune cell-mediated syndrome characterised by extensive inflammatory and effector mucosal responses leading to tissue destruction in the colon and small intestine. The leading hypothesis is that dysbiosis of the gut flora causes an excessive immune response and inflammation in the gastrointestinal track. Lactic acid bacteria (LAB) can correct dysbiosis of the normal microbiota. In the current study, the therapeutic potential of seven LAB strains in combination to treat IBD was evaluated using experimental colitis model. This LAB cocktail, designated GI7, includes four strains of Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus rhamnosus, Lactococcus lactis, two strains of Bifidobacterium bifidum, Bifidobacterium breve, and one strain of Streptococcus thermophilus. We confirmed that GI7 suppressed pro-inflammatory cytokines in Raw264.7 macrophages. When dextran sulphate sodium-induced colitic mice were treated with GI7, their symptoms of colitis, as assessed by body weight, colon length, myeloperoxidase activity, intestinal bleeding, and histological damage, were reduced compared to untreated mice. In addition, GI7 treatment significantly inhibited the production of innate pro-inflammatory cytokines during colitic progression. Therefore, we suggest that GI7, a combination of seven LAB, has a potential role in the treatment of IBD.
authors.affiliation: 1 R&D Center, Cell Biotech Co. Ltd., 134 Gaegok-Ri, Wolgot-Myeon Gimpo-Si, Gyeonggi-Do 10003, Republic of Korea.
authors.forename: M S
authors.initials: MS
authors.lastname: Kim
authors.affiliation: 1 R&D Center, Cell Biotech Co. Ltd., 134 Gaegok-Ri, Wolgot-Myeon Gimpo-Si, Gyeonggi-Do 10003, Republic of Korea.
authors.forename: J S
authors.initials: JS
authors.lastname: Byun
authors.affiliation: 1 R&D Center, Cell Biotech Co. Ltd., 134 Gaegok-Ri, Wolgot-Myeon Gimpo-Si, Gyeonggi-Do 10003, Republic of Korea.
authors.forename: Y S
authors.initials: YS
authors.lastname: Yoon
authors.affiliation: 1 R&D Center, Cell Biotech Co. Ltd., 134 Gaegok-Ri, Wolgot-Myeon Gimpo-Si, Gyeonggi-Do 10003, Republic of Korea.
authors.forename: D Y
authors.initials: DY
authors.lastname: Yum
authors.affiliation: 1 R&D Center, Cell Biotech Co. Ltd., 134 Gaegok-Ri, Wolgot-Myeon Gimpo-Si, Gyeonggi-Do 10003, Republic of Korea.
authors.forename: M J
authors.initials: MJ
authors.lastname: Chung
authors.affiliation: 1 R&D Center, Cell Biotech Co. Ltd., 134 Gaegok-Ri, Wolgot-Myeon Gimpo-Si, Gyeonggi-Do 10003, Republic of Korea.
authors.forename: J C
authors.initials: JC
authors.lastname: Lee
mesh_terms: D000818:Animals; D001644:Bifidobacterium / Q000145:classification*; D002460:Cell Line; D003092:Colitis / Q000139:chemically induced / Q000628:therapy*; D016207:Cytokines / Q000378:metabolism; D016264:Dextran Sulfate; D004195:Disease Models, Animal; D064806:Dysbiosis / Q000628:therapy*; D000069196:Gastrointestinal Microbiome / Q000276:immunology; D007249:Inflammation / Q000628:therapy; D007778:Lactobacillus / Q000145:classification*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D019936:Probiotics / Q000627:therapeutic use*; D000067996:RAW 264.7 Cells; D048249:Streptococcus thermophilus
chemical_list: D016207:Cytokines; D016264:Dextran Sulfate
keywords: cytokines; experimental colitis; inflammatory bowel disease; innate pro-inflammatory; lactic acid bacteria; probiotics