title: Investigation of the crosstalk between the flg22 and the UV-B-induced flavonol pathway in Arabidopsis thaliana seedlings.
abstract: In Arabidopsis cell culture, we have shown that flavonol metabolite accumulation depends on expression of the flavonol pathway genes (FPGs), which are up-regulated by UV-B irradiation but repressed during microbe-associated molecular pattern (MAMP) -triggered immunity (MTI) induced by the bacterial elicitor flg22. The suppression of flavonoid production during MTI is believed to allow the plant focusing its metabolism on the pathogen defense by directing phenylalanine resources from UV-B protective flavonol production towards production of phytoalexins and cell wall fortification by lignin incorporation. Here, we show that UV-B-induced FPGs are also suppressed by flg22 in Arabidopsis seedlings, demonstrating that this kind of 'crosstalk' is fully functional in planta. We believe that this system based on seedlings of the model plant Arabidopsis thaliana constitutes a valuable tool for further dissection of the underlying molecular mechanism, for example, by deploying gain/loss-of-function mutants. Furthermore, we observed some differences in the expression patterns of MYB transcription factors (TFs) as compared to data from the cell culture system. The new data suggest that in planta the TF MYB111 might play a more dominant role than the TF MYB12, which was strongly regulated in cell cultures. Thus, we can present an updated working model how this crosstalk might function.
authors.affiliation: Department of Molecular Phytopathology and Biotechnology, Christian-Albrechts University Kiel, Hermann-Rodewald Strasse 9, 24118, Kiel, Germany.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Molecular Phytopathology and Biotechnology, Christian-Albrechts University Kiel, Hermann-Rodewald Strasse 9, 24118, Kiel, Germany.
authors.forename: Dirk
authors.initials: D
authors.lastname: Schenke
authors.affiliation: Center for Molecular Cell Systems Biology, College of Life Sciences, Fujian Agriculture Forestry University, Fujian Fuzhou Jinshang Shangxiadian Road 15, Fuzhou, 350002, China.
authors.forename: Ying
authors.identifier: 0000-0003-2220-455X
authors.initials: Y
authors.lastname: Miao
authors.affiliation: Department of Molecular Phytopathology and Biotechnology, Christian-Albrechts University Kiel, Hermann-Rodewald Strasse 9, 24118, Kiel, Germany.
authors.forename: Daguang
authors.initials: D
authors.lastname: Cai
mesh_terms: D017360:Arabidopsis / Q000187:drug effects / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000378:metabolism; D001483:Base Sequence; D060146:Ecotype; D005408:Flagellin / Q000494:pharmacology*; D044948:Flavonols / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects / Q000528:radiation effects; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D008954:Models, Biological; D009154:Mutation / Q000235:genetics; D036226:Seedlings / Q000187:drug effects / Q000378:metabolism* / Q000528:radiation effects*; D014157:Transcription Factors / Q000378:metabolism; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; D044948:Flavonols; D035683:MicroRNAs; D014157:Transcription Factors; D005408:Flagellin
keywords: stress crosstalk