title: Structural, Evolutionary, and Functional Analysis of the Class III Peroxidase Gene Family in Chinese Pear (Pyrus bretschneideri).
abstract: Peroxidases (PRXs) are widely existed in various organisms and could be divided into different types according to their structures and functions. Specifically, the Class III Peroxidase, a plant-specific multi-gene family, involves in many physiological processes, such as the metabolism of auxin, the extension and thickening of cell wall, as well as the formation of lignin. By searching the pear genome database, 94 non-redundantPRXsfromPyrus bretschneideri(PbPRXs) were identified. Subsequently, analysis of phylogenetic relationships, gene structures, conserved motifs, and microsynteny was performed. ThesePbPRXswere unevenly distributed among 17 chromosomes of pear. In addition, 26 segmental duplication events but only one tandem duplication were occurred in thesePbPRXs, implying segmental duplication was the main contributor to the expansion of thePbPRXfamily. By the Ka/Ks analysis, 26 out of 27 duplicatedPbPRXshas experienced purifying selection. Twenty motifs were identified in PbPRXs based on the MEME analysis, 11 of which were enriched in pear. A total of 41 expressed genes were identified from ESTs of pear fruit. According to qRT-PCR, the expression trends of fivePbPRXsin subgroup C were consistent with the change of lignin content during pear fruit development. So we inferred that the fivePbPRXswere candidate genes involved in the lignin synthesis pathway. These results provided useful information for further researches ofPRXgenes in pear.
authors.affiliation: School of Life Sciences, Anhui Agricultural University Hefei, China.
authors.forename: Yunpeng
authors.initials: Y
authors.lastname: Cao
authors.affiliation: State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University Hefei, China.
authors.forename: Yahui
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Life Sciences, Anhui Agricultural University Hefei, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Meng
authors.affiliation: School of Life Sciences, Anhui Agricultural University Hefei, China.
authors.forename: Dahui
authors.initials: D
authors.lastname: Li
authors.affiliation: School of Life Sciences, Anhui Agricultural University Hefei, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Jin
authors.affiliation: School of Life Sciences, Anhui Agricultural University Hefei, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Lin
authors.affiliation: School of Life Sciences, Anhui Agricultural University Hefei, China.
authors.forename: Yongping
authors.initials: Y
authors.lastname: Cai
keywords: class III peroxidase; expression; gene duplication; microsynteny; pear