title: Assessment of the-174G/C(rs1800795) and-572G/C(rs1800796)Interleukin 6Gene Polymorphisms in Egyptian Patients with Rheumatoid Arthritis.
abstract: OBJECTIVE
  This study aimed to investigate genotype and allele frequencies of -174 (rs1800795) and-572(rs1800796)IL-6promoter gene polymorphisms in Egyptian patients with rheumatoid arthritis (RA) in comparison to control group.
  
  
  METHODS
  The study was conducted on 198 Egyptian subjects (99 RA patients and 99 healthy control). The promoter region ofthe IL-6gene was amplified by PCR using DNAs from patients and the controls, and their PCR products were digested by suitable enzymes.
  
  
  RESULTS
  No statistical differences were found in-572G/Cgenotype (P = 0.177) or allele (P = 0.147) frequencies between RA patients and controls. Significant differences were observed in-174G/Cgenotype (P < 0.001) and allele (P < 0.001) frequencies between RA patients and controls.
  
  
  CONCLUSIONS
  A significant association ofIL-6 -174G/Cgene polymorphism and RA in Egyptian population was found with significantly higher frequencies ofGCandCCgenotypes andCallele in RA patients compared to controls. No association was found betweenIL-6 -572G/Cgene polymorphism and RA.
authors.affiliation: National Research Center, Internal Medicine, Cairo, Egypt.
authors.forename: Khalda
authors.initials: K
authors.lastname: Amr
authors.affiliation: Al-Azhar University Faculty of Pharmacy, Biochemistry, Cairo, Egypt.
authors.forename: Rehab
authors.initials: R
authors.lastname: El-Awady
authors.affiliation: National Research Center, Internal Medicine, Cairo, Egypt.
authors.forename: Hala
authors.initials: H
authors.lastname: Raslan
keywords: IL6–174G/C; IL6–572G/C; Interleukin 6; Rheumatoid arthritis; gene polymorphism