title: Cysteamine supplementation revealed detrimental effect on cryosurvival of buffalo sperm based on computer-assisted semen analysis and oxidative parameters.
abstract: The aim of this study was to investigate the effect of addition of cysteamine to the semen extender on post-thaw semen quality. A total of 30 ejaculates were collected from six bulls. Each ejaculate was divided into five equal parts and diluted to final concentration of 80 million sperms/mL using Optixcell®(IMV, France) semen extender supplemented with different concentrations of cysteamine (0, 0.75, 1.25, 2.5 and 5mM) and cryopreserved. In the frozen-thawed samples, the VAP, VSL, VCL ALH and sperm motility of control samples was greater (P<0.05) than cysteamine treated samples. The sperm abnormality and malondialdehyde (MDA) concentration were found highest in 5mM cysteamine treated samples. The cysteamine treated samples travelled significantly less distance in cervical mucus as compared to control. Further, cysteamine decreased acrosomal integrity of sperm. In incubation test, control samples showed better sperm motility as compared to treatment groups. Further, cysteamine supplementation decreased the total antioxidants and increased the MDA concentration of sperm. From the study, we hypothesized that cysteamine cannot stimulate synthesis of glutathione (GSH) intracellularly in sperm to combat free radicals because during the maturation, sperm lost its cytoplasm which is necessary for biochemical reaction in which cysteamine reacts with cystine to form a mixed disulfide which taken up by cells and split into cysteine in the cytoplasm. Synthesis of GSH depends on the availability of cysteine. In conclusion, the results of our study strongly emphasize that cysteamine would not be a suitable additive in extender for freezing buffalo bull semen.
authors.affiliation: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125001, India; Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
authors.forename: Dheer Singh
authors.initials: DS
authors.lastname: Swami
authors.affiliation: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125001, India. Electronic address: drpradeepkrvet@gmail.com.
authors.forename: Pradeep
authors.initials: P
authors.lastname: Kumar
authors.affiliation: Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Malik
authors.affiliation: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125001, India.
authors.forename: Monika
authors.initials: M
authors.lastname: Saini
authors.affiliation: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125001, India.
authors.forename: Dharmendra
authors.initials: D
authors.lastname: Kumar
authors.affiliation: Animal Physiology and Reproduction Division, ICAR-Central Institute for Research on Buffaloes, Hisar, Haryana 125001, India.
authors.forename: M H
authors.initials: MH
authors.lastname: Jan
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D002020:Buffaloes; D002470:Cell Survival / Q000187:drug effects; D015925:Cryopreservation / Q000379:methods / Q000662:veterinary; D003543:Cysteamine / Q000009:adverse effects* / Q000494:pharmacology; D005978:Glutathione / Q000378:metabolism; D007091:Image Processing, Computer-Assisted; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects* / Q000502:physiology; D055101:Semen Analysis / Q000379:methods / Q000662:veterinary; D012662:Semen Preservation / Q000009:adverse effects / Q000379:methods / Q000662:veterinary; D013081:Sperm Motility / Q000187:drug effects; D013094:Spermatozoa / Q000187:drug effects* / Q000502:physiology
chemical_list: D000975:Antioxidants; D008315:Malondialdehyde; D003543:Cysteamine; D005978:Glutathione
keywords: Buffalo; CASA; Cryopreservation; Cysteamine; Semen quality