title: Corning HYPERFlask®for viral amplification and production of diagnostic reagents.
abstract: Viral preparations are essential components in diagnostic research and development. The production of large quantities of virus traditionally is done by infecting numerous tissue culture flasks or roller bottles, which require large incubators and/or roller bottle racks. The Corning HYPERFlask®is a multilayer flask that uses a gas permeable film to provide gas exchange between the cells and culture medium and the atmospheric environment. This study evaluated the suitability of the HYPERFlask for production of Lassa, Ebola, Bundibugyo, Reston, and Marburg viruses and compared it to more traditional methods using tissue culture flasks and roller bottles. The HYPERFlask produced cultures were equivalent in virus titer and indistinguishable in immunodiagnostic assays. The use of the Corning HYPERFlask for viral production is a viable alternative to traditional tissue culture flasks and roller bottles. HYPERFlasks allow for large volumes of virus to be produced in a small space without specialized equipment.
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD, 21702, United States.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Kearney
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD, 21702, United States.
authors.forename: Matthew A
authors.initials: MA
authors.lastname: Voorhees
authors.affiliation: Ke'aki Technologies, LLC, 1425 Porter Street, Fort Detrick, MD, 21702, United States.
authors.forename: Priscilla L
authors.initials: PL
authors.lastname: Williams
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD, 21702, United States.
authors.forename: Scott P
authors.initials: SP
authors.lastname: Olschner
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD, 21702, United States.
authors.forename: Cynthia A
authors.initials: CA
authors.lastname: Rossi
authors.affiliation: Diagnostic Systems Division, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), 1425 Porter Street, Fort Detrick, MD, 21702, United States. Electronic address: randal.j.schoepp.civ@mail.mil.
authors.forename: Randal J
authors.initials: RJ
authors.lastname: Schoepp
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003470:Culture Media; D029043:Ebolavirus / Q000254:growth & development* / Q000302:isolation & purification; D007836:Lassa virus / Q000254:growth & development* / Q000302:isolation & purification; D029024:Marburgvirus / Q000254:growth & development* / Q000302:isolation & purification; D014709:Vero Cells; D014776:Virus Cultivation / Q000295:instrumentation* / Q000379:methods; D014779:Virus Replication
chemical_list: D003470:Culture Media
keywords: Bundibugyo; ELISA; Ebola; HYPERFlask; Immunodiagnostic; Lassa; Marburg; Reston; Virus amplification