title: Expression of miRNA-155, miRNA-223, miRNA-31, miRNA-21, miRNA-125b, and miRNA-146a in the Inflammatory Pathway of Hidradenitis Suppurativa.
abstract: Hidradenitis suppurativa (HS) has been associated with marked inflammatory perturbation. The mechanisms regulating the inflammatory network remain elusive. microRNAs (miRNAs) have been described as gene regulators of inflammation. We evaluated the messenger RNA (mRNA) expression levels of six selected inflammation-related miRNAs in lesional and perilesional skin samples of HS patients and in healthy controls. Samples of 15 HS patients and 10 healthy controls were included in this prospective study. Expression levels of the miRNAs miRNA-155-5p, miRNA-223-5p, miRNA-31-5p, miRNA-21-5p, miRNA-125b-5p, and miRNA-146a-5p were studied by quantitative real-time reverse transcription polymerase chain reaction. We observed a significant overexpression of miRNA-155-5p, miRNA-223-5p, miRNA-31-5p, miRNA-21-5p, and miRNA-146a-5p in lesional HS skin compared to healthy controls. Expression of these miRNAs was also significantly increased in lesional HS skin when compared to perilesional skin. Only miRNA-155-5p showed an increased expression in perilesional skin compared to healthy controls. In contrast, miRNA-125b-5p had a significantly lower expression in lesional HS skin compared to perilesional skin. We found that the studied inflammation-related miRNAs were significantly dysregulated in lesional HS skin and may have regulatory roles in the inflammatory process of HS. Given their predicted targets and functions, our findings point to these miRNAs as potential disease biomarkers, and manipulation might be used therapeutically to target the inflammatory pathway in HS.
authors.affiliation: Dermatologic Surgery Department, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany.
authors.forename: S
authors.identifier: http://orcid.org/0000-0001-6547-6577
authors.initials: S
authors.lastname: Hessam
authors.affiliation: Dermatologic Surgery Department, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Sand
authors.affiliation: Dermatologic Surgery Department, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Skrygan
authors.affiliation: Dermatologic Surgery Department, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Gambichler
authors.affiliation: Dermatologic Surgery Department, Department of Dermatology, Venereology and Allergology, Ruhr-University Bochum, Gudrunstr. 56, 44791, Bochum, Germany. f.bechara@klinikum-bochum.de.
authors.forename: F G
authors.initials: FG
authors.lastname: Bechara
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D005260:Female; D005786:Gene Expression Regulation; D017497:Hidradenitis Suppurativa / Q000235:genetics* / Q000473:pathology; D006801:Humans; D007249:Inflammation / Q000235:genetics*; D008297:Male; D053858:Metabolic Networks and Pathways / Q000235:genetics; D035683:MicroRNAs / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D011446:Prospective Studies; D012867:Skin / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C531888:MIRN125 microRNA, human; C542542:MIRN146 microRNA, human; C524942:MIRN155 microRNA, human; C524940:MIRN21 microRNA, human; C525067:MIR223, human; C534612:MIRN31 microRNA, human
keywords: hidradenitis suppurativa; inflammation-related microRNAs; inflammatory skin disease; microRNA