title: Exosomal miR-486 regulates hypoxia-induced erythroid differentiation of erythroleukemia cells through targeting Sirt1.
abstract: MicroRNAs (miRNAs) regulate the hypoxia-induced erythroid differentiation of hematopoietic cells. In this study, we identified that miR-486 was a rapid response miRNA to hypoxia in erythroleukemia TF-1 cells. Hypoxia exposure increased both intracellular and miR-486 levels of TF-1 cells. Ectopic miR-486 expression enhanced the growth and erythroid differentiation of TF-1 cells, whereas miR-486 inhibition suppressed their growth and erythroid differentiation. Treatment of TF-1 and cord blood CD34+ cells with exogenous containing miR-486 resulted in an increase of intracellular miR-486 level and enhanced erythroid differentiation. Furthermore, we identified that Sirt1 is a miR-486 target gene which modulates hypoxia-induced erythroid differentiation of TF-1 cells. Thus we identified a novel miRNA regulatory network that contributes to hypoxia-induced erythroid differentiation of hematopoietic cells.
authors.affiliation: High Altitude Medicine of Ministry of Chinese Education and Research Center for High Altitude Medicine, Qinghai University, Xining 810001, PR China; Qinghai Provincial People's Hospital, Xining 810007, PR China.
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Shi
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
authors.forename: Fan-Xuan
authors.initials: FX
authors.lastname: Kong
authors.affiliation: High Altitude Medicine of Ministry of Chinese Education and Research Center for High Altitude Medicine, Qinghai University, Xining 810001, PR China; Qinghai Provincial People's Hospital, Xining 810007, PR China.
authors.forename: Qin-Qin
authors.initials: QQ
authors.lastname: Xu
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
authors.forename: Feng-Jun
authors.initials: FJ
authors.lastname: Xiao
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China.
authors.forename: Yue-Feng
authors.initials: YF
authors.lastname: Yang
authors.affiliation: High Altitude Medicine of Ministry of Chinese Education and Research Center for High Altitude Medicine, Qinghai University, Xining 810001, PR China. Electronic address: geriligao@hotmail.com.
authors.forename: Ri-Li
authors.initials: RL
authors.lastname: Ge
authors.affiliation: Department of Experimental Hematology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China; Beijing Key Laboratory for Radiobiology, Beijing Institute of Radiation Medicine, Beijing 100850, PR China; School of Nursing, Jilin University, Changchun, Jilin 130021, PR China. Electronic address: wangls@bmi.ac.cn.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
mesh_terms: D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D002478:Cells, Cultured; D015672:Erythroid Precursor Cells / Q000166:cytology* / Q000378:metabolism; D004920:Erythropoiesis; D006801:Humans; D004915:Leukemia, Erythroblastic, Acute / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D010100:Oxygen / Q000378:metabolism; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism
chemical_list: C560945:MIRN486 microRNA, human; D035683:MicroRNAs; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D010100:Oxygen
keywords: Erythroid differentiation; Exosomes; Hypoxia; TF-1 cells; miR-486