title: Optimization of desferrioxamine E production by Streptomyces parvulus.
abstract: Siderophores are produced by a number of microbes to capture iron with outstandingly high affinity, which property also generates biomedical and industrial interests. Desferrioxamine E (DFO-E) secreted by streptomycetes bacteria can be an ideal candidate for iron chelation therapy, which necessitates its cost-effective production for in vitro and animal studies. This study focused on the optimization of DFO-E production by Streptomyces parvulus CBS548.68. Different combinations of various carbon and nitrogen sources as well as the addition of 3-morpholinopropane-1-sulfonic acid (MOPS) markedly affected DFO-E yields, which were attributed, at least in part, to the higher biomass productions found in MOPS-supplemented cultures. In MOPS-supplemented glucose and sodium glutamate medium, DFO-E productions as high as 2,009 ± 90 mg/l of culture medium were reached. High-performance liquid chromatography analysis demonstrated that a simple two-step purification process yielded DFO-E preparations with purities of ∼97%. Matrix assisted laser desorption ionization-time of flight mass spectrometry analysis showed that purified DFO-E always contained traces of desferrioxamine D2.
authors.affiliation: 1 MTA-DE Vascular Biology, Thrombosis and Hemostasis Research Group, Hungarian Academy of Sciences , Debrecen, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Gáll
authors.affiliation: 2 Department of Inorganic and Analytical Chemistry, Faculty of Science and Technology, University of Debrecen , Debrecen, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Lehoczki
authors.affiliation: 2 Department of Inorganic and Analytical Chemistry, Faculty of Science and Technology, University of Debrecen , Debrecen, Hungary.
authors.forename: Gyöngyi
authors.initials: G
authors.lastname: Gyémánt
authors.affiliation: 3 Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen , Debrecen, Hungary.
authors.forename: Tamás
authors.initials: T
authors.lastname: Emri
authors.affiliation: 3 Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen , Debrecen, Hungary.
authors.forename: Zsuzsa M
authors.initials: ZM
authors.lastname: Szigeti
authors.affiliation: 1 MTA-DE Vascular Biology, Thrombosis and Hemostasis Research Group, Hungarian Academy of Sciences , Debrecen, Hungary.|4 Department of Pediatrics, Faculty of Medicine, University of Debrecen , Debrecen, Hungary.
authors.forename: György
authors.initials: G
authors.lastname: Balla
authors.affiliation: 5 Department of Internal Medicine, Faculty of Medicine, University of Debrecen , Debrecen, Hungary.
authors.forename: József
authors.initials: J
authors.lastname: Balla
authors.affiliation: 3 Department of Biotechnology and Microbiology, Faculty of Science and Technology, University of Debrecen , Debrecen, Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Pócsi
mesh_terms: D002851:Chromatography, High Pressure Liquid; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D006877:Hydroxamic Acids / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D007218:Industrial Microbiology; D007769:Lactams / Q000032:analysis / Q000302:isolation & purification / Q000378:metabolism*; D013302:Streptomyces / Q000737:chemistry / Q000378:metabolism*
chemical_list: D003470:Culture Media; D006877:Hydroxamic Acids; D007769:Lactams; C561576:desferrioxamine E
keywords: MOPS; Na-glutamate; Streptomyces parvulus; desferrioxamine E