title: Intraarticular and intravenous administration of99MTc-HMPAO-labeled human mesenchymal stem cells (99MTC-AH-MSCS): In vivo imaging and biodistribution.
abstract: BACKGROUND
  Therapeutic application of intravenous administered (IV) human bone marrow-derived mesenchymal stem cells (ahMSCs) appears to have as main drawback the massive retention of cells in the lung parenchyma, questioning the suitability of this via of administration. Intraarticular administration (IAR) could be considered as an alternative route for therapy in degenerative and traumatic joint lesions. Our work is outlined as a comparative study of biodistribution of99mTc-ahMSCs after IV and IAR administration, via scintigraphic study in an animal model.
  
  
  METHODS
  Isolated primary culture of adult human mesenchymal stem cells was labeled with99mTc-HMPAO for scintigraphic study of in vivo distribution after intravenous and intra-articular (knee) administration in rabbits.
  
  
  RESULTS
  IV administration of radiolabeled ahMSCs showed the bulk of radioactivity in the lung parenchyma while IAR images showed activity mainly in the injected cavity and complete absence of uptake in pulmonary bed.
  
  
  CONCLUSIONS
  Our study shows that IAR administration overcomes the limitations of IV injection, in particular, those related to cells destruction in the lung parenchyma. After IAR administration, cells remain within the joint cavity, as expected given its size and adhesion properties.
  
  
  CONCLUSIONS
  Intra-articular administration of adult human mesenchymal stem cells could be a suitable route for therapeutic effect in joint lesions.
  
  
  CONCLUSIONS
  Local administration of adult human mesenchymal stem cells could improve their therapeutic effects, minimizing side effects in patients.
authors.affiliation: Servicio de Ortopedia y Traumatología, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain; UCAM Universidad Católica San Antonio de Murcia, 30130, Murcia, Spain.
authors.forename: Luis
authors.initials: L
authors.lastname: Meseguer-Olmo
authors.affiliation: Servicio de Medicina Nuclear, Clínica Belén, 30008, Murcia, Spain.
authors.forename: Antonio Jesús
authors.initials: AJ
authors.lastname: Montellano
authors.affiliation: Unidad de Radiofarmacia, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain. Electronic address: tmmtnez@gmail.com.
authors.forename: Teresa
authors.initials: T
authors.lastname: Martínez
authors.affiliation: Unidad de Patología Experimental, IMIB-LAIB Arrixaca, CIBERehd, 30120, Murcia, Spain.
authors.forename: Carlos M
authors.initials: CM
authors.lastname: Martínez
authors.affiliation: Unidad de Genómica, IMIB-LAIB Arrixaca, CIBERehd, 30120, Murcia, Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: Revilla-Nuin
authors.affiliation: Unidad de Radiofarmacia, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Roldán
authors.affiliation: Department of Neurology, New York University School of Medicine, New York, NY, USA.
authors.forename: Cristina Fuente
authors.initials: CF
authors.lastname: Mora
authors.affiliation: Unidad de Terapia Celular, Hospital Universitario V. Arrixaca, 30120, Murcia, Spain.
authors.forename: Maria Dolores
authors.initials: MD
authors.lastname: López-Lucas
authors.affiliation: Unidad de Radiofarmacia, Hospital Clínico Universitario Virgen de la Arrixaca, Murcia, Spain.
authors.forename: Teodomiro
authors.initials: T
authors.lastname: Fuente
mesh_terms: D061605:Administration, Intravenous; D006801:Humans; D007553:Isotope Labeling; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D057054:Molecular Imaging / Q000379:methods*; D019690:Technetium Tc 99m Exametazime / Q000008:administration & dosage* / Q000378:metabolism / Q000493:pharmacokinetics*; D014018:Tissue Distribution
chemical_list: D019690:Technetium Tc 99m Exametazime
keywords: (99m)Tc-ahMSCs; Biodistribution; Intra-articular; Intravenous; Mesenchymal stem cells; Scintigraphy