title: Overexpression of Mitofusin2 decreased the reactive astrocytes proliferation in vitro induced by oxygen-glucose deprivation/reoxygenation.
abstract: Glia scar is a hallmark in late-stage of brain stroke disease, which hinder axonal regeneration and neuronal repair. Mitofusin2 (Mfn2) is a newly found cellular proliferation inhibitor. This study is to elucidate the role of Mfn2 in reactive astrocytes induced by oxygen-glucose deprivation/reoxygenation(OGD/R) model in vitro. Up-expression in EdU staining and protein level of GFAP, PCNA and CyclinD1, demonstrates the distinct activation and proliferation of astrocytes after the stimulation of OGD/R. Meanwhile, Mfn2 was proved to be down-regulated both in gene and protein levels. Pretreatment of cells with adenoviral vector encoding Mfn2 gene increased Mfn2 expression and subsequently attenuated OGD-induced astrocyte proliferation. Down-regulation of Ras-p-Raf1-p-ERK1/2 pathway and cell cycle arrest were found to be relevant. Together, these results suggested that overexpression of Mfn2 can effectively inhibit the proliferation of reactive astrogliosis, which might contribute to a promising therapeutic intervention in cerebral ischemic injury.
authors.affiliation: Department of Traumatic Surgery, Tong-ji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Jie Fang Avenue 1095, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Department of Traumatic Surgery, Tong-ji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Jie Fang Avenue 1095, China. Electronic address: chenglayi@163.com.
authors.forename: Chengla
authors.initials: C
authors.lastname: Yi
authors.affiliation: Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Traumatic Surgery, Tong-ji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Jie Fang Avenue 1095, China.
authors.forename: Jiangpeng
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Traumatic Surgery, Tong-ji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Jie Fang Avenue 1095, China.
authors.forename: Fangyuan
authors.initials: F
authors.lastname: Zhou
authors.affiliation: Department of Pathophysiology, School of Basic Medicine, Key Laboratory of Neurological Diseases, Ministry of Education and Hubei Provincial Key Laboratory of Neurological Diseases, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Chen
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001253:Astrocytes / Q000378:metabolism*; D015687:Cell Hypoxia / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D020558:GTP Phosphohydrolases; D005947:Glucose / Q000378:metabolism*; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism*; D024101:Mitochondrial Proteins / Q000235:genetics / Q000378:metabolism*; D010100:Oxygen / Q000378:metabolism*; D017207:Rats, Sprague-Dawley
chemical_list: D008565:Membrane Proteins; D024101:Mitochondrial Proteins; D020558:GTP Phosphohydrolases; C489710:Mfn2 protein, rat; D005947:Glucose; D010100:Oxygen
keywords: Astrocyte; Cell-cycle; Ischemia；Proliferation; Mitofusin2; Ras-p-Raf1-p-ERK1/2