title: Genome sequence and overview ofOligoflexus tunisiensisShr3Tin the eighth classOligoflexiaof the phylumProteobacteria.
abstract: Oligoflexus tunisiensisShr3Tis the first strain described in the newest (eighth) classOligoflexiaof the phylumProteobacteria. This strain was isolated from the 0.2-μm filtrate of a suspension of sand gravels collected in the Sahara Desert in the Republic of Tunisia. The genome ofO. tunisiensisShr3Tis 7,569,109 bp long and consists of one scaffold with a 54.3% G + C content. A total of 6,463 genes were predicted, comprising 6,406 protein-coding and 57 RNA genes. Genome sequence analysis suggested that strain Shr3Thad multiple terminal oxidases for aerobic respiration and various transporters, including the resistance-nodulation-cell division-type efflux pumps. Additionally, gene sequences related to the incomplete denitrification pathway lacking the final step to reduce nitrous oxide (N2O) to nitrogen gas (N2) were found in theO. tunisiensisShr3Tgenome. The results presented herein provide insight into the metabolic versatility and N2O-producing activity ofOligoflexusspecies.
authors.affiliation: Genetic Strains Research Center, National Institute of Genetics, 1111 Yata, Mishima, 411-8540 Japan.
authors.forename: Ryosuke
authors.initials: R
authors.lastname: Nakai
authors.affiliation: Center for Information Biology, National Institute of Genetics, 1111 Yata, Mishima, 411-8540 Japan.
authors.forename: Takatomo
authors.initials: T
authors.lastname: Fujisawa
authors.affiliation: Center for Information Biology, National Institute of Genetics, 1111 Yata, Mishima, 411-8540 Japan ; Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, 411-8540 Japan.
authors.forename: Yasukazu
authors.initials: Y
authors.lastname: Nakamura
authors.affiliation: Genetic Strains Research Center, National Institute of Genetics, 1111 Yata, Mishima, 411-8540 Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Baba
authors.affiliation: Technical Department, TechnoSuruga Laboratory Co., Ltd., 330 Nagasaki, Shimizu-ku, Shizuoka, 424-0065 Japan.
authors.forename: Miyuki
authors.initials: M
authors.lastname: Nishijima
authors.affiliation: Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018 Sfax, Tunisia.
authors.forename: Fatma
authors.initials: F
authors.lastname: Karray
authors.affiliation: Centre of Biotechnology of Sfax, University of Sfax, Route Sidi Mansour, km 6, BP 1177, 3018 Sfax, Tunisia.
authors.forename: Sami
authors.initials: S
authors.lastname: Sayadi
authors.affiliation: Alliance for Research on North Africa (ARENA), University of Tsukuba, 1-1-1 Tennoudai, Tsukuba, 305-8572 Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Isoda
authors.affiliation: Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-hiroshima, 739-8528 Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Naganuma
authors.affiliation: Genetic Strains Research Center, National Institute of Genetics, 1111 Yata, Mishima, 411-8540 Japan ; Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), 1111 Yata, Mishima, 411-8540 Japan.
authors.forename: Hironori
authors.initials: H
authors.lastname: Niki
keywords: Denitrification; Nitrous oxide (N2O); Oligoflexia; Proteobacteria; RND-type efflux pump