title: Casein Kinase 1 Coordinates Cohesin Cleavage, Gametogenesis, and Exit from M Phase in Meiosis II.
abstract: Meiosis consists of DNA replication followed by two consecutive nuclear divisions and gametogenesis or spore formation. While meiosis I has been studied extensively, less is known about the regulation of meiosis II. Here we show that Hrr25, the conserved casein kinase 1δ of budding yeast, links three mutually independent key processes of meiosis II. First, Hrr25 induces nuclear division by priming centromeric cohesin for cleavage by separase. Hrr25 simultaneously phosphorylates Rec8, the cleavable subunit of cohesin, and removes from centromeres the cohesin protector composed of shugoshin and the phosphatase PP2A. Second, Hrr25 initiates the sporulation program by inducing the synthesis of membranes that engulf the emerging nuclei at anaphase II. Third, Hrr25 mediates exit from meiosis II by activating pathways that trigger the destruction of M-phase-promoting kinases. Thus, Hrr25 synchronizes formation of the single-copy genome with gamete differentiation and termination of meiosis.
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Orlando
authors.initials: O
authors.lastname: Argüello-Miranda
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Ievgeniia
authors.initials: I
authors.lastname: Zagoriy
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Valentina
authors.initials: V
authors.lastname: Mengoli
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Julie
authors.initials: J
authors.lastname: Rojas
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Jonak
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Tugce
authors.initials: T
authors.lastname: Oz
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Graf
authors.affiliation: Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany. Electronic address: zachar@biochem.mpg.de.
authors.forename: Wolfgang
authors.initials: W
authors.lastname: Zachariae
mesh_terms: D000705:Anaphase; D047389:Casein Kinase I / Q000378:metabolism*; D018797:Cell Cycle Proteins / Q000378:metabolism*; D002467:Cell Nucleus / Q000378:metabolism; D002503:Centromere / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism*; D005718:Gametogenesis; D008540:Meiosis; D010766:Phosphorylation; D054648:Protein Phosphatase 2 / Q000378:metabolism; D059748:Proteolysis; D012441:Saccharomyces cerevisiae / Q000166:cytology* / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D064247:Separase / Q000378:metabolism; D008941:Spindle Apparatus / Q000378:metabolism; D000097722:Cohesins
chemical_list: D047389:Casein Kinase I; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D054648:Protein Phosphatase 2; D029701:Saccharomyces cerevisiae Proteins; D064247:Separase; D000097722:Cohesins; C070775:HRR25 protein, S cerevisiae
keywords: PP2A; Rec8 cleavage by separase; casein kinase 1; exit from meiosis; gametogenesis; meiosis II; protection of centromeric cohesin; shugoshin; spindle disassembly; sporulation