title: Comparative Aroma Extract Dilution Analysis (cAEDA) of Fat from Tainted Boars, Castrated Male Pigs, and Female Pigs.
abstract: The aroma profile of porcine fat from tainted boars, female pigs, and castrated male pigs was investigated by application of comparative aroma extract dilution analysis (cAEDA) on a SAFE distillate of volatiles prepared from porcine back fat samples. The AEDA resulted in a total of 16 aroma active compounds for boar fat with flavor dilution (FD) factors ranging from 2 to 2048, whereas 12 aroma active compounds were found in fat of female pigs and 14 in fat of castrated male pigs, both with FD factors ranging from 2 to 32. Odor activity values (OAVs) of key components for each fat were identified: In boar fat androstenone, skatole, indole, and 2-aminoacetophenone showed highest OAVs, whereas 2,5-dimethylpyrazine, 2,4-decadienal, and δ-decalactone showed highest OAVs in fat of female pigs. Fat of castrated male pigs showed highest OAVs for skatole, indole, 1-octen-3-ol and methional. Finally, the off-flavor attributes of boar fat were successfully simulated by a recombinant of all odorants at their natural concentration level in deodorized sunflower oil.
authors.affiliation: Institute of Nutritional and Food Sciences, Chair of Bioanalytics , University of Bonn , Endenicher Allee 11-13 , D-53115 Bonn , Germany.|ELFI Analytik GbR , Massenhausener Straße 18a , D-85375 Neufahrn , Germany.
authors.forename: Christoph
authors.initials: C
authors.lastname: Gerlach
authors.affiliation: Institute of Agricultural Engineering , University of Bonn , Nußallee 5 , D-53115 Bonn , Germany.
authors.forename: Jan
authors.initials: J
authors.lastname: Leppert
authors.affiliation: Institute of Nutritional and Food Sciences, Chair of Bioanalytics , University of Bonn , Endenicher Allee 11-13 , D-53115 Bonn , Germany.
authors.forename: Alicia Chamarro
authors.initials: AC
authors.lastname: Santiuste
authors.affiliation: Institute of Nutritional and Food Sciences, Chair of Bioanalytics , University of Bonn , Endenicher Allee 11-13 , D-53115 Bonn , Germany.
authors.forename: Anne
authors.initials: A
authors.lastname: Pfeiffer
authors.affiliation: Institute of Agricultural Engineering , University of Bonn , Nußallee 5 , D-53115 Bonn , Germany.
authors.forename: Peter
authors.initials: P
authors.lastname: Boeker
authors.affiliation: Institute of Nutritional and Food Sciences, Chair of Bioanalytics , University of Bonn , Endenicher Allee 11-13 , D-53115 Bonn , Germany.
authors.forename: Matthias
authors.identifier: http://orcid.org/0000-0001-6808-5555
authors.initials: M
authors.lastname: Wüst
mesh_terms: D000818:Animals; D005223:Fats / Q000737:chemistry*; D005260:Female; D008401:Gas Chromatography-Mass Spectrometry; D007201:Indicator Dilution Techniques; D008297:Male; D009812:Odorants / Q000032:analysis; D012903:Smell; D034421:Sus scrofa; D013552:Swine; D055549:Volatile Organic Compounds / Q000737:chemistry*
chemical_list: D005223:Fats; D055549:Volatile Organic Compounds
keywords: aroma extract dilution analysis; aroma recombination; boar taint; odor activity values