title: SFPQ associates to LSD1 and regulates the migration of newborn pyramidal neurons in the developing cerebral cortex.
abstract: The development of the cerebral cortex requires the coordination of multiple processes ranging from the proliferation of progenitors to the migration and establishment of connectivity of the newborn neurons. Epigenetic regulation carried out by the COREST/LSD1 complex has been identified as a mechanism that regulates the development of pyramidal neurons of the cerebral cortex. We now identify the association of the multifunctional RNA-binding protein SFPQ to LSD1 during the development of the cerebral cortex. In vivo reduction of SFPQ dosage by in utero electroporation of a shRNA results in impaired radial migration of newborn pyramidal neurons, in a similar way to that observed when COREST or LSD1 expressions are decreased. Diminished SFPQ expression also associates to decreased proliferation of progenitor cells, while it does not affect the acquisition of neuronal fate. These results are compatible with the idea that SFPQ, plays an important role regulating proliferation and migration during the development of the cerebral cortex.
authors.affiliation: Program in Physiology and Biophysics, Institute for Biomedical Sciences, and Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors.forename: K
authors.initials: K
authors.lastname: Saud
authors.affiliation: Program in Physiology and Biophysics, Institute for Biomedical Sciences, and Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors.forename: J
authors.initials: J
authors.lastname: Cánovas
authors.affiliation: Program in Physiology and Biophysics, Institute for Biomedical Sciences, and Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors.forename: C I
authors.initials: CI
authors.lastname: Lopez
authors.affiliation: Program in Physiology and Biophysics, Institute for Biomedical Sciences, and Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors.forename: F A
authors.initials: FA
authors.lastname: Berndt
authors.affiliation: Program in Physiology and Biophysics, Institute for Biomedical Sciences, and Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile.
authors.forename: E
authors.initials: E
authors.lastname: López
authors.affiliation: Program in Physiology and Biophysics, Institute for Biomedical Sciences, and Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile; Department of Otolaryngology, Hospital Clínico Universidad de Chile, Santiago, Chile; Department of Surgery, Faculty of Medicine Clínica Alemana-Universidad del Desarrollo, Santiago, Chile.
authors.forename: J C
authors.initials: JC
authors.lastname: Maass
authors.affiliation: Mass Spectrometry Unit-Center of Studies for the Development of Chemistry (CEPEDEQ), Faculty of Chemical and Pharmaceutical Sciences, Universidad de Chile, Santiago, Chile.
authors.forename: A
authors.initials: A
authors.lastname: Barriga
authors.affiliation: Program in Physiology and Biophysics, Institute for Biomedical Sciences, and Biomedical Neuroscience Institute, Faculty of Medicine, Universidad de Chile, Santiago, Chile. Electronic address: kukuljan@med.uchile.cl.
authors.forename: M
authors.initials: M
authors.lastname: Kukuljan
mesh_terms: D000367:Age Factors; D000818:Animals; D000831:Animals, Newborn; D002460:Cell Line; D002465:Cell Movement / Q000235:genetics / Q000502:physiology*; D002540:Cerebral Cortex / Q000166:cytology / Q000196:embryology / Q000254:growth & development; D000089622:Doublecortin Domain Proteins; D018274:Electroporation; D004622:Embryo, Mammalian; D018507:Gene Expression Regulation, Developmental / Q000235:genetics / Q000502:physiology; D056466:Histone Demethylases / Q000235:genetics / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000378:metabolism; D019394:Ki-67 Antigen / Q000378:metabolism; D051379:Mice; D008869:Microtubule-Associated Proteins / Q000378:metabolism; D009479:Neuropeptides / Q000378:metabolism; D009687:Nuclear Proteins / Q000378:metabolism; D000072670:PTB-Associated Splicing Factor / Q000235:genetics / Q000378:metabolism*; D017966:Pyramidal Cells / Q000502:physiology*; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism; D055748:SOXB1 Transcription Factors / Q000378:metabolism
chemical_list: C000624101:Cux1 protein, mouse; D000089622:Doublecortin Domain Proteins; D018398:Homeodomain Proteins; D019394:Ki-67 Antigen; D008869:Microtubule-Associated Proteins; D009479:Neuropeptides; D009687:Nuclear Proteins; D000072670:PTB-Associated Splicing Factor; D034741:RNA, Small Interfering; D012097:Repressor Proteins; D055748:SOXB1 Transcription Factors; C527783:Sox2 protein, mouse; D056466:Histone Demethylases; C504076:KDM1a protein, mouse
keywords: Corticogenesis; Epigenetic regulation; Neuronal migration