title: Effects of Raloxifene on the Proliferation and Apoptosis of Human Aortic Valve Interstitial Cells.
abstract: We aimed to explore the effects of raloxifene (RAL) on the proliferation and apoptosis of human aortic valve interstitial cells (AVICs). Different concentrations of RAL were used to act on AVICs. MTS kit is used to test the effects of different concentrations of RAL on the proliferation of AVICs. Cell cycle and apoptosis test used flow cytometry after seven-day treatment. The relative expression levels of caspase-3 and caspase-8 are tested with RT-qPCR and Western blot. The results of MTS testing revealed that the absorbance value (OD value) of the cells in the concentration groups of 10 and 100 nmol/L RAL at a wavelength of 490 nm at five, seven, and nine days significantly decreased compared with that in the control group. Meanwhile, the results of flow cytometry of the cells collected after seven days showed that the ratio of the S stage and the cell apoptosis rate of AVICs can be significantly reduced by RAL in the concentration groups of 10 and 100 nmol/L. The mRNA and protein expressions of caspase-3 and caspase-8 were significantly decreased compared with those in the control group. This study laid the foundation for further treatment of aortic valve disease by using RAL.
authors.affiliation: Department of Cardiothoracic Surgery, The First Affiliated Hospital of Jinan University, Guangzhou Shi, China; Department of Cardiothoracic Surgery, Chenzhou First People's Hospital of Hunan Province, Chenzhou 423000, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Department of Cardiac Surgery, Shenzhen People's Hospital, Shenzhen 518020, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Luo
authors.affiliation: Cardiovascular Department, Chenzhou First People's Hospital of Hunan Province, Chenzhou 423000, China.
authors.forename: Mingpeng
authors.initials: M
authors.lastname: Li
authors.affiliation: Cardiovascular Department, Chenzhou First People's Hospital of Hunan Province, Chenzhou 423000, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Peng
authors.affiliation: Department of Cardiothoracic Surgery, The 2nd Clinical Medical College of Jinan University, Shenzhen 518020, China; Department of Cardiothoracic Surgery, Shenzhen People's Hospital, Shenzhen 518020, China.
authors.forename: Zheng
authors.identifier: 0000-0001-9252-2129
authors.initials: Z
authors.lastname: Wang
mesh_terms: D001021:Aortic Valve / Q000166:cytology / Q000201:enzymology*; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000096:biosynthesis*; D053181:Caspase 8 / Q000096:biosynthesis*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D005260:Female; D015971:Gene Expression Regulation, Enzymologic / Q000187:drug effects*; D006801:Humans; D008875:Middle Aged; D020849:Raloxifene Hydrochloride / Q000494:pharmacology*; D013997:Time Factors
chemical_list: D020849:Raloxifene Hydrochloride; C505407:CASP3 protein, human; C505530:CASP8 protein, human; D053148:Caspase 3; D053181:Caspase 8