title: Spatiotemporal Patterns of RING1 Expression after Rat Spinal Cord Injury.
abstract: Ring finger protein 1 (RING1) is a RING domain characterized protein belonging to the RING finger family. It is an E3 ubiquitin-protein ligase that mediated monoubiquitination of histone H2A and the core component of PRC1 complex, which is the repressive multiprotein complex of Polycomb group (PcG). Previous studies showed the important tumorigenic role of RING1 via promoting cell proliferation and the crucial function in maintaining transcriptional program stability during development. However, its mechanism for spinal cord injury (SCI) is still unknown. In our research, we established an acute SCI model in adult rats and studied the expression and function profiles of RING1. RING1 protein level detected by western blot peaked at day 3 after trauma and then decreased gradually. Immunohistochemistry showed the increase of RING1 expression displayed in the white matter more obviously than in the gray matter. Furthermore, increased co-expression of RING1 and GFAP confirmed activated astrocytes in injured spinal cord via double immunofluorescence staining. Meanwhile, we also found the co-localization of PCNA, a famous marker of proliferative cells, with RING1 and GFAP, which indicated RING1 might play a role in astrocyte proliferation after SCI. In vitro studies, RING1 protein level in C6 cells increased after LPS challenge and RING1 was required for astrocyte proliferation and activation induced by LPS. In summary, we took a new insight into the function of RING1 in the cellular and molecular mechanism underlying the pathophysiology of SCI.
authors.affiliation: Morphology Laboratory, Medical College of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Hanzhang
authors.initials: H
authors.lastname: Liu
authors.affiliation: Key Laboratory of Nerve Regeneration, Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ji
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Gong
authors.affiliation: Experimental Animal Center of Nantong University, Medical College of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Chun
authors.initials: C
authors.lastname: Liu
authors.affiliation: Morphology Laboratory, Medical College of Nantong University, Nantong, 226001, Jiangsu, China.|Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Neurosurgery, Affiliated Hospital of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Yilu
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Experimental Animal Center of Nantong University, Medical College of Nantong University, Nantong, 226001, Jiangsu, China. ntdxzsx@163.com.
authors.forename: Shunxing
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Nerve Regeneration, Nantong University, Nantong, 226001, Jiangsu, China. 13861980557@163.com.
authors.forename: Leilei
authors.initials: L
authors.lastname: Gong
mesh_terms: D000818:Animals; D015870:Gene Expression; D008297:Male; D009457:Neuroglia / Q000378:metabolism* / Q000473:pathology; D063150:Polycomb Repressive Complex 1 / Q000096:biosynthesis* / Q000235:genetics; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000166:cytology / Q000378:metabolism / Q000473:pathology; D013119:Spinal Cord Injuries / Q000235:genetics / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D063150:Polycomb Repressive Complex 1; C570469:Ring1 protein, rat; D044767:Ubiquitin-Protein Ligases
keywords: Activation; Astrocyte; Proliferation; RING1; SCI