title: The genome sequence of Brassica campestris chrysovirus 1, a novel putative plant-infecting tripartite chrysovirus.
abstract: A putative chrysovirus recovered from Brassica campestris var. purpurea and provisionally named "Brassica campestris chrysovirus 1" (BrcCV1) was sequenced. The genome of the putative BrcCV1 consists of three double-stranded RNAs (dsRNAs) comprising 3,639 (dsRNA 1), 3,567 (dsRNA 2) and 3,337 (dsRNA 3) base pairs, respectively, each containing a single open reading frame (ORF 1-3). The putative proteins encoded by ORF 1-3 show homologies to RdRp, CP and chryso-P3 of approved or tentative chrysoviruses. In addition, the three dsRNAs of BrcCV1 contain highly conserved 5' and 3' untranslated regions (UTRs) in a way similar to known chrysoviruses. In a phylogenetic tree based on the conserved amino acid sequences of the RdRps of chrysoviruses, totiviruses and partitiviruses, the putative BrcCV1 formed a separate clade with Raphanus sativus chrysovirus 1 (RasCV1), a putative trisegmented, plant-infecting chrysovirus, in the family Chrysoviridae.
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.|Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.|Hunan Plant Protection Institute, Changsha, 410125, Hunan, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Zhao
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Hu
authors.affiliation: College of Chemistry and environmental Engineering, Yangtze University, Jingzhou, 434023, Hubei, China.
authors.forename: Lingfang
authors.initials: L
authors.lastname: Guo
authors.affiliation: Hunan Plant Protection Institute, Changsha, 410125, Hunan, China.|Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Limin
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Zhenguo
authors.initials: Z
authors.lastname: Du
authors.affiliation: Fujian Province Key Laboratory of Plant Virology, Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, Fujian, China.
authors.forename: Zujian
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China.|Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China.
authors.forename: Shouguo
authors.initials: S
authors.lastname: Fang
authors.affiliation: Engineering Research Centre of Ecology and Agricultural Use of Wetland, Ministry of Education, Yangtze University, Jingzhou, 434025, Hubei, China. yangtze2008@126.com.|Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, China. yangtze2008@126.com.
authors.forename: Songbai
authors.identifier: http://orcid.org/0000-0002-8425-1434
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Hunan Plant Protection Institute, Changsha, 410125, Hunan, China. haoasliu@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D001483:Base Sequence; D001937:Brassica / Q000821:virology*; D016679:Genome, Viral; D016366:Open Reading Frames; D010802:Phylogeny; D010935:Plant Diseases / Q000821:virology*; D012328:RNA Viruses / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D012367:RNA, Viral / Q000235:genetics; D014764:Viral Proteins / Q000235:genetics
chemical_list: D020413:3' Untranslated Regions; D012367:RNA, Viral; D014764:Viral Proteins