title: Non-culture based diagnostics for intravascular catheter related bloodstream infections.
abstract: intravascular catheter related bloodstream infection (IVC-BSI) is a leading cause of nosocomial infections and associated with significant morbidity and mortality. Early detection and adequate treatment of causative pathogens is critical for a favourable outcome. However, it takes significant time to receive microbiological results due to the current reference diagnostic method's reliance on microbial growth. Areas covered: This review discusses culture and non-culture based techniques for the diagnosis of non IVC-BSI and IVC-BSI, including molecular methods and biomarkers. Different diagnostic strategies are evaluated and the potential of new generation of diagnostic assays highlighted. Expert commentary: The development of additional diagnostic methods has potential to beneficially supplement conventional culture diagnosis, and molecular techniques have particular potential to fulfil this need. They would also contribute significant new knowledge on the bacterial species present on catheters that are generally missed by diagnosis using traditionally culture-dependent methods. Advances in molecular strategies, together with new biomarkers, might lead to the development of faster, more sensitive and cheaper technologies and instruments. This review aims to provide a platform for the further development of IVCBSI diagnostic techniques.
authors.affiliation: a Griffith University Menzies Health Institute Queensland , Alliance for Vascular Access Teaching and Research (AVATAR) , Nathan , Australia.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: a Griffith University Menzies Health Institute Queensland , Alliance for Vascular Access Teaching and Research (AVATAR) , Nathan , Australia.
authors.forename: C M
authors.initials: CM
authors.lastname: Rickard
mesh_terms: D001424:Bacterial Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology*; D015373:Bacterial Typing Techniques / Q000295:instrumentation / Q000379:methods*; D015415:Biomarkers / Q000097:blood; D055499:Catheter-Related Infections / Q000097:blood / Q000175:diagnosis* / Q000382:microbiology*; D006801:Humans
chemical_list: D015415:Biomarkers
keywords: Intravascular catheter; blood culture; catheter related bloodstream infection; diagnosis; molecular methods