title: Virus Infections on Prion Diseased Mice Exacerbate Inflammatory Microglial Response.
abstract: We investigated possible interaction between an arbovirus infection and the ME7 induced mice prion disease. C57BL/6, females, 6-week-old, were submitted to a bilateral intrahippocampal injection of ME7 prion strain (ME7) or normal brain homogenate (NBH). After injections, animals were organized into two groups: NBH (n= 26) and ME7 (n= 29). At 15th week after injections (wpi), animals were challenged intranasally with a suspension of Piry arbovirus 0.001% or with NBH. Behavioral changes in ME7 animals appeared in burrowing activity at 14 wpi. Hyperactivity on open field test, errors on rod bridge, and time reduction in inverted screen were detected at 15th, 19th, and 20th wpi respectively. Burrowing was more sensitive to earlier hippocampus dysfunction. However, Piry-infection did not significantly affect the already ongoing burrowing decline in the ME7-treated mice. After behavioral tests, brains were processed for IBA1, protease-resistant form of PrP, and Piry virus antigens. Although virus infection in isolation did not change the number of microglia in CA1, virus infection in prion diseased mice (at 17th wpi) induced changes in number and morphology of microglia in a laminar-dependent way. We suggest that virus infection exacerbates microglial inflammatory response to a greater degree in prion-infected mice, and this is not necessarily correlated with hippocampal-dependent behavioral deficits.
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Nara
authors.initials: N
authors.lastname: Lins
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Luiz
authors.initials: L
authors.lastname: Mourão
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Nonata
authors.initials: N
authors.lastname: Trévia
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Aline
authors.initials: A
authors.lastname: Passos
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: José Augusto
authors.initials: JA
authors.lastname: Farias
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Jarila
authors.initials: J
authors.lastname: Assunção
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Amanda
authors.identifier: 0000-0002-9567-0829
authors.initials: A
authors.lastname: Quintairos
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: João
authors.initials: J
authors.lastname: Bento-Torres
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Marcia
authors.initials: M
authors.lastname: Consentino Kronka Sosthenes
authors.affiliation: Instituto Evandro Chagas, Laboratório de Microscopia Eletrônica and Departamento de Arbovirologia e Febres Hemorrágicas Virais, Belém, Brazil.
authors.forename: José Antonio Picanço
authors.initials: JA
authors.lastname: Diniz
authors.affiliation: Instituto Evandro Chagas, Laboratório de Microscopia Eletrônica and Departamento de Arbovirologia e Febres Hemorrágicas Virais, Belém, Brazil.
authors.forename: Pedro Fernando da Costa
authors.initials: PF
authors.lastname: Vasconcelos
authors.affiliation: Universidade Federal do Pará, Instituto de Ciências Biológicas, Laboratório de Investigações em Neurodegeneração e Infecção no Hospital Universitário João de Barros Barreto, Belém, Brazil.
authors.forename: Cristovam Wanderley
authors.identifier: 0000-0001-6611-6880
authors.initials: CW
authors.lastname: Picanço-Diniz
mesh_terms: D000818:Animals; D000956:Antigens, Viral / Q000276:immunology; D001103:Arboviruses / Q000276:immunology / Q000472:pathogenicity*; D001522:Behavior, Animal; D056547:CA1 Region, Hippocampal / Q000276:immunology / Q000473:pathology / Q000503:physiopathology / Q000821:virology*; D002135:Calcium-Binding Proteins / Q000378:metabolism; D060085:Coinfection; D004195:Disease Models, Animal; D018450:Disease Progression; D004671:Encephalitis, Arbovirus / Q000150:complications* / Q000276:immunology / Q000473:pathology / Q000523:psychology; D005260:Female; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism; D017628:Microglia / Q000276:immunology / Q000473:pathology / Q000821:virology*; D009043:Motor Activity; D009410:Nerve Degeneration; D017096:Prion Diseases / Q000150:complications* / Q000473:pathology / Q000523:psychology; D013997:Time Factors
chemical_list: C491049:Aif1 protein, mouse; D000956:Antigens, Viral; D002135:Calcium-Binding Proteins; D008840:Microfilament Proteins