title: Bioengineered Osteoinductive Broussonetia kazinoki/Silk Fibroin Composite Scaffolds for Bone Tissue Regeneration.
abstract: In this article, Broussonetia kazinoki (BK) powdery extract is utilized to modify the silk fibroin (SF) scaffold and applied to the bone defect area. The BK/SF scaffold is an efficient cell carrier which promotes cell proliferation and osteogenic differentiation of rBMSCs (bone marrow derived mesenchymal stem cells). We confirmed biocompatibility and osteogenic differentiation capacity of BK/SF scaffolds compared to pristine SF scaffold in both in vitro and in vivo evaluation. Gene expression related to osteogenic differentiation and bone regeneration significantly upregulated in the BK/SF scaffold group. The implanted scaffolds were attached well to the surface of the bone defect region and integrated with surrounding tissues without significant inflammatory reaction. Furthermore, almost 45% of bone volume has been recovered at 8 weeks postsurgery, while the SF and control group showed 20% recovery. These results suggest that BK powdery extract incorporated with an SF scaffold might be a suitable substitute for an alternative bone graft for bone regeneration.
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University , Deokjin-gu, Jeonju 561-756, Republic of Korea.
authors.forename: Do Kyung
authors.initials: DK
authors.lastname: Kim
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University , Jeonju 561-756, Republic of Korea.
authors.forename: Jeong In
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Department of Bionanosystem Engineering, Graduate School, Chonbuk National University , Jeonju 561-756, Republic of Korea.
authors.forename: Tae In
authors.initials: TI
authors.lastname: Hwang
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University , Deokjin-gu, Jeonju 561-756, Republic of Korea.
authors.forename: Bo Ra
authors.initials: BR
authors.lastname: Sim
authors.affiliation: Department of BIN Convergence Technology, Department of Polymer Nano Science & Technology and Polymer BIN Research Center, Chonbuk National University , Deokjin-gu, Jeonju 561-756, Republic of Korea.
authors.forename: Gilson
authors.identifier: http://orcid.org/0000-0002-6452-5653
authors.initials: G
authors.lastname: Khang
mesh_terms: D001861:Bone Regeneration; D031623:Broussonetia / Q000737:chemistry*; D005349:Fibroins / Q000737:chemistry*; D059630:Mesenchymal Stem Cells; D010012:Osteogenesis; D023822:Tissue Engineering; D054457:Tissue Scaffolds
chemical_list: D005349:Fibroins
keywords: Broussonetia kazinoki; bone regeneration; plant extract; scaffold; silk fibroin