title: WIND1 Promotes Shoot Regeneration through Transcriptional Activation ofENHANCER OF SHOOT REGENERATION1in Arabidopsis.
abstract: Many plant species display remarkable developmental plasticity and regenerate new organs after injury. Local signals produced by wounding are thought to trigger organ regeneration but molecular mechanisms underlying this control remain largely unknown. We previously identified an AP2/ERF transcription factor WOUND INDUCED DEDIFFERENTIATION1 (WIND1) as a central regulator of wound-induced cellular reprogramming in plants. In this study, we demonstrate that WIND1 promotes callus formation and shoot regeneration by upregulating the expression of theENHANCER OF SHOOT REGENERATION1(ESR1) gene, which encodes another AP2/ERF transcription factor inArabidopsis thalianaTheesr1mutants are defective in callus formation and shoot regeneration; conversely, its overexpression promotes both of these processes, indicating that ESR1 functions as a critical driver of cellular reprogramming. Our data show that WIND1 directly binds the vascular system-specific and wound-responsivecis-element-like motifs within theESR1promoter and activates its expression. The expression ofESR1is strongly reduced inWIND1-SRDXdominant repressors, and ectopic overexpression ofESR1bypasses defects in callus formation and shoot regeneration inWIND1-SRDXplants, supporting the notion that ESR1 acts downstream of WIND1. Together, our findings uncover a key molecular pathway that links wound signaling to shoot regeneration in plants.
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
authors.forename: Akira
authors.identifier: 0000-0003-3294-7939
authors.initials: A
authors.lastname: Iwase
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
authors.forename: Hirofumi
authors.identifier: 0000-0003-3370-4111
authors.initials: H
authors.lastname: Harashima
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
authors.forename: Momoko
authors.identifier: 0000-0001-9474-5131
authors.initials: M
authors.lastname: Ikeuchi
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
authors.forename: Bart
authors.identifier: 0000-0003-3651-9579
authors.initials: B
authors.lastname: Rymen
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Ohnuma
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan.
authors.forename: Shinichiro
authors.identifier: 0000-0002-1189-288X
authors.initials: S
authors.lastname: Komaki
authors.affiliation: Center for Applied Plant Sciences and Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210.
authors.forename: Kengo
authors.identifier: 0000-0003-1200-8432
authors.initials: K
authors.lastname: Morohashi
authors.affiliation: Graduate School of Biological Sciences, Nara Institute of Science and Technology, Ikoma 630-0192, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Kurata
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Nakata
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Tsukuba 305-8562, Japan.|Graduate School of Science and Engineering, Saitama University, Saitama 338-8570, Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Ohme-Takagi
authors.affiliation: Center for Applied Plant Sciences and Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210.
authors.forename: Erich
authors.identifier: 0000-0002-4720-7290
authors.initials: E
authors.lastname: Grotewold
authors.affiliation: RIKEN Center for Sustainable Resource Science, Yokohama 230-0045, Japan keiko.sugimoto@riken.jp.
authors.forename: Keiko
authors.identifier: 0000-0002-9209-8230
authors.initials: K
authors.lastname: Sugimoto
mesh_terms: D017360:Arabidopsis / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D029681:Arabidopsis Proteins / Q000235:genetics* / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018613:Microscopy, Confocal; D018520:Plant Shoots / Q000235:genetics* / Q000378:metabolism / Q000502:physiology; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D012038:Regeneration / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000235:genetics; D046509:Tissue Culture Techniques; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D015533:Transcriptional Activation
chemical_list: D029681:Arabidopsis Proteins; C450147:ESR1 protein, Arabidopsis; D014157:Transcription Factors; C559637:WIND1 protein, Arabidopsis