title: A comparison report of three advanced methods for drug-cyclodextrin interaction measurements.
abstract: Three advanced methods, high performance affinity chromatography (HPAC), surface plasmon resonance (SPR) and surface plasmon resonance imaging (SPRi) were compared and evaluated for determining the drug-cyclodextrin (CD) interactions herein. In total, 18 sparingly soluble drugs were selected for this comparative study. The three methods share a unique connection in the working principles and strategies. The same strategies of CD fixation onto solid phase were used in HPAC and SPR for the measurements, whereas, the SPR and SPRi share identical working principles. However, whilst these relationships are evident, no strong correlation was found between kinetic constants obtained from the three methods: Four drugs, namely, prednisolone, pseudolaric acid B, diazepam and gramisetron failed to show any response on SPR, whereas, the kinetics parameters from SPRi and HPAC were successfully measured. From a comparative review of all the kinetic data, random results without any trends were observed (ka, kd and KA) regardless of the relationships between the three methods: It is apparent that the measurement conditions (volume, flow rate, buffers), non-specific adsorption and experimental procedures had a strong impact on the generated data. The relative advantages and limitations of each method are critically presented on the basis of generated data. This comparative study provides a basis to further upgrade these techniques for confident measurement of drug-CDs interactions.
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Vikramjeet
authors.initials: V
authors.lastname: Singh
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Yaping
authors.initials: Y
authors.lastname: He
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Caifen
authors.initials: C
authors.lastname: Wang
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Jianghui
authors.initials: J
authors.lastname: Xu
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Xu
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Li
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.
authors.forename: Parbeen
authors.initials: P
authors.lastname: Singh
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Institute of Pharmaceutical Innovation, University of Bradford, Bradford, West Yorkshire BD71DP, United Kingdom.
authors.forename: Peter
authors.initials: P
authors.lastname: York
authors.affiliation: Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China. Electronic address: slx04@163.com.
authors.forename: Lixin
authors.initials: L
authors.lastname: Sun
authors.affiliation: Center for Drug Delivery Systems, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: jwzhang@simm.ac.cn.
authors.forename: Jiwen
authors.initials: J
authors.lastname: Zhang
mesh_terms: D003505:Cyclodextrins / Q000032:analysis* / Q000378:metabolism*; D004347:Drug Interactions; D020349:Surface Plasmon Resonance / Q000379:methods* / Q000592:standards; D053719:Tandem Mass Spectrometry / Q000379:methods* / Q000592:standards
chemical_list: D003505:Cyclodextrins
keywords: Drug-cyclodextrins interactions; High performance affinity chromatography; Kinetic constants; Sparingly soluble drugs; Surface plasmon resonance; Surface plasmon resonance imaging