title: Differential expression of Toll-like receptors in goat dominant and nondominant follicles.
abstract: The mechanism of dominant follicle selection is unclear because of its physiological complexity. However, some studies have reported that the immune system plays an important role in reproductive physiology. The objective of the current study was to investigate the differential expression of Toll-like receptors (TLRs) in the dominant (DFs) and nondominant follicles (NFs), and to determine the correlation between the expression of TLRs and the related genes, such as WNT4 and FOXL2. In this comparative study, the expression levels of TLRs, WNT4, and FOXL2 genes of DFs and NFs were obtained from three Dazu black goats were estimated using the real-time PCR. Our results showed no significant difference in the expression of seven TLRs (excluding TLR2, TLR5, and TLR8), WNT4, and FOXL2 between the DFs and NFs. In addition, the mRNA expression levels of WNT4 significantly correlated with the relative expression of TLR6 (r = 0.949739, P < 0.01); however, no significant expression of the TLR genes was found to be associated with FOXL2 mRNA expression. Our results support the fact that TLRs are not involved in the process of dominant follicle selection; however, TLR6 might play a role in the development of follicles by interacting with WNT4.
authors.affiliation: College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China.
authors.forename: G X
authors.initials: GX
authors.lastname: E
authors.affiliation: College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China.
authors.forename: Y J
authors.initials: YJ
authors.lastname: Zhao
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Y H
authors.initials: YH
authors.lastname: Ma
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: M X
authors.initials: MX
authors.lastname: Chu
authors.affiliation: Yunnna Animal Science and Veterinary Institute, Kunming, China.
authors.forename: Q H
authors.initials: QH
authors.lastname: Hong
authors.affiliation: College of Life Sciences, Linyi University, Linyi, Shandong, China.
authors.forename: S J
authors.initials: SJ
authors.lastname: Lv
authors.affiliation: Yunnna Animal Science and Veterinary Institute, Kunming, China.
authors.forename: L
authors.initials: L
authors.lastname: Zhu
authors.affiliation: College of Life Sciences, Linyi University, Linyi, Shandong, China.
authors.forename: F K
authors.initials: FK
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China.
authors.forename: Y G
authors.initials: YG
authors.lastname: Han
authors.affiliation: Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: X
authors.initials: X
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Chongqing Key Laboratory of Forage & Herbivore, Chongqing Engineering Research Centre for Herbivores Resource Protection and Utilization, Southwest University, Chongqing, China H67738337@swu.edu.cn.
authors.forename: Y F
authors.initials: YF
authors.lastname: Huang
mesh_terms: D000818:Animals; D005260:Female; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors / Q000235:genetics*; D015870:Gene Expression; D006041:Goats / Q000235:genetics*; D018859:Hair Follicle / Q000276:immunology*; D040641:Quantitative Trait Loci; D060888:Real-Time Polymerase Chain Reaction; D051193:Toll-Like Receptors / Q000235:genetics*; D060528:Wnt4 Protein / Q000235:genetics*
chemical_list: C421604:FOXL2 protein, human; D000074842:Forkhead Box Protein L2; D051858:Forkhead Transcription Factors; D051193:Toll-Like Receptors; D060528:Wnt4 Protein