title: Design and characterization of a magnetite/PEI multifunctional nanohybrid as non-viral vector and cell isolation system.
abstract: It is described the reproducible formulation and complete physicochemical characterization of nanohybrids based on magnetite (Fe3O4) cores embedded within a polyethylenimine (PEI) matrix. Particle size, surface electrical charge, X-ray diffraction and Fourier transform infrared spectroscopy (FTIR) analyses, and magnetic field-responsive behaviour characterizations defined that the 4:3 (Fe3O4:PEI) weight proportion led to the best production performances of magnetically responsive nanocomposites in which the magnetic nuclei are completely covered by the polymeric shell. Agarose gel electrophoresis assays demonstrated the capacity of the Fe3O4/PEI particles to condense, release, and protect the DNA against enzymatic degradation. In vitro assays were performed to evaluate the transfection efficiency (up to 4.5% of transfected HEK-293 cells at a 10/1 PEI/DNA ratio), iron absorption by D1-mesenchymal stem cells (D1-MSCs, high values after only 15min of magnetic incubation), influence on metabolic activity (negligible effect up to 44μg nanocomposites/105cells), and cell isolation capacity of the core/shell particles (significant increase in the retention of D1-MSCs transduced with green fluorescent protein). The Fe3O4/PEI nanohybrids hold promising characteristics suggestive of their capacity for transfection and cell isolation applications.
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain.
authors.forename: Raúl
authors.initials: R
authors.lastname: Megías
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Maialen
authors.initials: M
authors.lastname: Arco
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Jesús
authors.initials: J
authors.lastname: Ciriza
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Laura
authors.initials: L
authors.lastname: Saenz Del Burgo
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Puras
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain.
authors.forename: Margarita
authors.initials: M
authors.lastname: López-Viota
authors.affiliation: Department of Applied Physics, Faculty of Sciences, University of Granada, Granada, Spain.
authors.forename: Ángel V
authors.initials: ÁV
authors.lastname: Delgado
authors.affiliation: Department of Biomedical Engineering and Medical Physics, Institute for Science & Technology in Medicine, Keele University, Staffordshire, United Kingdom.
authors.forename: Jon P
authors.initials: JP
authors.lastname: Dobson
authors.affiliation: Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Granada, Granada, Spain; Institute of Biopathology and Regenerative Medicine (IBIMER), University of Granada, Granada, Spain; Biosanitary Institute of Granada (ibs.GRANADA), Andalusian Health Service (SAS), University of Granada, Granada, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Arias
authors.affiliation: NanoBioCel Group, University of the Basque Country (UPV/EHU), Vitoria-Gasteiz, Spain; Biomedical Research Networking Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Vitoria-Gasteiz, Spain. Electronic address: joseluis.pedraz@ehu.es.
authors.forename: José L
authors.initials: JL
authors.lastname: Pedraz
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D004247:DNA / Q000008:administration & dosage / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000008:administration & dosage / Q000737:chemistry*; D049452:Green Fluorescent Proteins / Q000235:genetics; D057809:HEK293 Cells; D006801:Humans; D059630:Mesenchymal Stem Cells; D051379:Mice; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D010316:Particle Size; D011094:Polyethyleneimine / Q000008:administration & dosage / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014162:Transfection; D014961:X-Ray Diffraction
chemical_list: D049452:Green Fluorescent Proteins; D011094:Polyethyleneimine; D004247:DNA; D052203:Ferrosoferric Oxide
keywords: Cell enrichment; Gene therapy; Magnetic composite; Magnetite; Polyethylenimine; Transfection