title: Three-Dimensional Ordered Antibody Arrays Through Self-Assembly of Antibody-Polymer Conjugates.
abstract: Three-dimensional (3D) ordered arrays of human immunoglobulin G (IgG) were fabricated using well-defined full-length antibody-polymer conjugates (APCs). The conjugates were prepared through a two-step sequential click approach with a combination of oxime ligation and strain promoted alkyne-azide cycloaddition. They were able to self-assemble into lamellar nanostructures with alternating IgG and poly(N-isopropylacrylamide) (PNIPAM) nanodomains. As a proof-of-concept, these materials were fabricated into thin films and their specific binding ability was tested. The nanostructure not only improves the packing density and the proper orientation of the IgG, but also provides nanochannels to facilitate substrate transport.
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Xue-Hui
authors.initials: XH
authors.lastname: Dong
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Allie C
authors.initials: AC
authors.lastname: Obermeyer
authors.affiliation: Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Bradley D
authors.identifier: 0000-0002-7272-7140
authors.initials: BD
authors.lastname: Olsen
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000480:Alkynes / Q000737:chemistry; D001386:Azides / Q000737:chemistry; D002384:Catalysis; D003300:Copper / Q000737:chemistry; D061565:Cycloaddition Reaction; D006801:Humans; D007074:Immunoglobulin G / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D049329:Nanostructures / Q000737:chemistry; D053838:Scattering, Small Angle; D014961:X-Ray Diffraction
chemical_list: D000180:Acrylic Resins; D000480:Alkynes; D001386:Azides; D007074:Immunoglobulin G; C052970:poly-N-isopropylacrylamide; D003300:Copper
keywords: antibodies; click chemistry; conjugates; nanostructures; self-assembly