title: A field evaluation of an isothermal DNA amplification assay for the detection of Theileria annulata infection in cattle.
abstract: A loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Theileria annulata infection in cattle. The results were compared with a real-time PCR used for the quantification of T. annulata parasitaemia. One hundred bovine blood samples from 16 cattle farms were tested with LAMP and real-time PCR, with T. annulata DNA being detected in 66% and 67% of the samples, respectively. The results showed that the LAMP assay detects a parasitaemia as low as 0.00025%, indicating a high analytical sensitivity of LAMP for clinical diagnosis of bovine theileriosis.
authors.affiliation: Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal, Instituto Nacional de Investigação Agrária e Veterinária, I.P., Lisbon, Portugal. Electronic address: jacinto.gomes@iniav.pt.
authors.forename: Jacinto
authors.initials: J
authors.lastname: Gomes
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: Marcos
authors.initials: M
authors.lastname: Santos
authors.affiliation: Unidade Estratégica de Investigação e Serviços em Produção e Saúde Animal, Instituto Nacional de Investigação Agrária e Veterinária, I.P., Lisbon, Portugal.
authors.forename: Ana
authors.initials: A
authors.lastname: Amaro
authors.affiliation: Centro de Investigação Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterinária, Universidade de Lisboa, Lisbon, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Pereira da Fonseca
authors.affiliation: Global Health and Tropical Medicine Centre (GHTM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Santos-Gomes
authors.affiliation: Global Health and Tropical Medicine Centre (GHTM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Lisbon, Portugal; School of Pharmacy and Biomolecular Sciences, University of Brighton, Brighton, United Kingdom.
authors.forename: João
authors.initials: J
authors.lastname: Inácio
mesh_terms: D000818:Animals; D002417:Cattle / Q000469:parasitology*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D012015:Reference Standards; D016796:Theileria annulata / Q000235:genetics* / Q000302:isolation & purification*; D013801:Theileriasis / Q000469:parasitology*
keywords: Bovine theileriosis; LAMP; Molecular diagnostics; Real-time PCR; Tams1; Theileria annulata