title: Arctigenin protects against neuronal hearing loss by promoting neural stem cell survival and differentiation.
abstract: Neuronal hearing loss has become a prevalent health problem. This study focused on the function of arctigenin (ARC) in promoting survival and neuronal differentiation of mouse cochlear neural stem cells (NSCs), and its protection against gentamicin (GMC) induced neuronal hearing loss. Mouse cochlea was used to isolate NSCs, which were subsequently cultured in vitro. The effects of ARC on NSC survival, neurosphere formation, differentiation of NSCs, neurite outgrowth, and neural excitability in neuronal network in vitro were examined. Mechanotransduction ability demonstrated by intact cochlea, auditory brainstem response (ABR), and distortion product optoacoustic emissions (DPOAE) amplitude in mice were measured to evaluate effects of ARC on GMC-induced neuronal hearing loss. ARC increased survival, neurosphere formation, neuron differentiation of NSCs in mouse cochlear in vitro. ARC also promoted the outgrowth of neurites, as well as neural excitability of the NSC-differentiated neuron culture. Additionally, ARC rescued mechanotransduction capacity, restored the threshold shifts of ABR and DPOAE in our GMC ototoxicity murine model. This study supports the potential therapeutic role of ARC in promoting both NSCs proliferation and differentiation in vitro to functional neurons, thus supporting its protective function in the therapeutic treatment of neuropathic hearing loss in vivo.
authors.affiliation: Department of Ultrasound Diagnosis, the Second Xiangya Hospital, Central South University, 139 Renmin Road, Changsha, Hunan, 410011, China.
authors.forename: Xinghua
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the Second Xiangya Hospital, Central South University, 139 Renmin Road, Changsha, Hunan, 410011, China.
authors.forename: Mo
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the Second Xiangya Hospital, Central South University, 139 Renmin Road, Changsha, Hunan, 410011, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Department of Otolaryngology-Head and Neck Surgery, the Second Xiangya Hospital, Central South University, 139 Renmin Road, Changsha, Hunan, 410011, China.
authors.forename: Qin
authors.identifier: 0000-0003-1115-4905
authors.initials: Q
authors.lastname: Wang
mesh_terms: D000818:Animals; D002470:Cell Survival; D002478:Cells, Cultured; D003051:Cochlea / Q000166:cytology / Q000187:drug effects; D005072:Evoked Potentials, Auditory; D005663:Furans / Q000494:pharmacology* / Q000627:therapeutic use; D005839:Gentamicins / Q000633:toxicity; D034381:Hearing Loss / Q000188:drug therapy* / Q000209:etiology; D017705:Lignans / Q000494:pharmacology* / Q000627:therapeutic use; D040542:Mechanotransduction, Cellular; D051379:Mice; D008807:Mice, Inbred BALB C; D058953:Neural Stem Cells / Q000166:cytology / Q000187:drug effects*; D055495:Neurogenesis; D009474:Neurons / Q000187:drug effects / Q000502:physiology
chemical_list: D005663:Furans; D005839:Gentamicins; D017705:Lignans; C071942:arctigenin
keywords: arctigenin; cochlea; differentiation; hearing loss; neural stem cell