title: Determination and speciation of cadmium in microcosms with Bunodosoma caissarum and Perna perna using isotopically enriched116Cd.
abstract: The study of the uptake and distribution of elements in marine environments is of great interest for understanding their pathways and accumulation. Here, we investigated in laboratory experiments the accumulation behavior of Cd in the sea anemone Bunodosoma caissarum and the mussel Perna perna. Specimens were incubated with isotopically enriched116Cd in aquariums. Cd concentrations in the seawater and in the tissues of B. caissarum and P. perna were followed by inductively coupled plasma-mass spectrometry (ICP-MS) by means of isotope dilution analysis. Bioconcentration factors for B. caissarum and P. perna exposed to 0.9μg·L-1of116Cd were determined to be 80.5 and 850, respectively. P. perna specimens exposed to 4.5μg·L-1of116Cd reached 530. Cytosolic proteins associated with Cd from the tissues were extracted and further analyzed by size-exclusion chromatography coupled to ICP-MS. Cd accumulation could be detected in both organisms ranging from high-molecular to low-molecular species.
authors.affiliation: Departamento de Geoquímica, Universidade Federal Fluminense, Outeiro de São João Batista s/n, Instituto de Química, 5° andar, Centro, Niterói, RJ 24020-141, Brazil. Electronic address: nafisarizzini@yahoo.com.
authors.forename: Nafisa
authors.initials: N
authors.lastname: Rizzini Ansari
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, C/Julian Claveria 8, 33006 Oviedo, Spain.
authors.forename: Nerea
authors.initials: N
authors.lastname: Fernández Iglesias
authors.affiliation: Departamento de Geoquímica, Universidade Federal Fluminense, Outeiro de São João Batista s/n, Instituto de Química, 5° andar, Centro, Niterói, RJ 24020-141, Brazil.
authors.forename: Renato Campello
authors.initials: RC
authors.lastname: Cordeiro
authors.affiliation: Faculdade de Oceanografia, Universidade do Estado do Rio de Janeiro, São Francisco Xavier St. 524, 4018E, 20550-013 Maracanã, RJ, Brazil.
authors.forename: Marcos Antônio
authors.initials: MA
authors.lastname: Fernandez
authors.affiliation: Department of Physical and Analytical Chemistry, University of Oviedo, C/Julian Claveria 8, 33006 Oviedo, Spain. Electronic address: bettmerjorg@uniovi.es.
authors.forename: Jörg
authors.initials: J
authors.lastname: Bettmer
mesh_terms: D000818:Animals; D002104:Cadmium / Q000378:metabolism*; D013058:Mass Spectrometry; D049883:Perna / Q000378:metabolism*; D012615:Sea Anemones / Q000378:metabolism*; D012623:Seawater
chemical_list: D002104:Cadmium
keywords: Bunodosoma caissarum; Cadmium; Elemental speciation; Inductively coupled plasma-mass spectrometry (ICP-MS); Isotope dilution analysis (IDA); Isotope pattern deconvolution (IPD); Perna perna