title: Mechanical Criterion for the Rupture of a Cell Membrane under Compression.
abstract: We investigate the mechanical conditions leading to the rupture of the plasma membrane of an endothelial cell subjected to a local, compressive force. Membrane rupture is induced by tilted microindentation, a technique used to perform mechanical measurements on adherent cells. In this technique, the applied force can be deduced from the measured horizontal displacement of a microindenter's tip, as imaged with an inverted microscope and without the need for optical sensors to measure the microindenter's deflection. We show that plasma membrane rupture of endothelial cells occurs at a well-defined value of the applied compressive stress. As a point of reference, we use numerical simulations to estimate the magnitude of the compressive stresses exerted on endothelial cells during the deployment of a stent.
authors.affiliation: Laboratoire de Chimie et Physique - Approche Multi-échelles des Milieux Complexes, Université de Lorraine, Metz, France.
authors.forename: David
authors.initials: D
authors.lastname: Gonzalez-Rodriguez
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR 7646, Department of Mechanics, École Polytechnique, Palaiseau, France.
authors.forename: Lionel
authors.initials: L
authors.lastname: Guillou
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR 7646, Department of Mechanics, École Polytechnique, Palaiseau, France.
authors.forename: François
authors.initials: F
authors.lastname: Cornat
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR 7646, Department of Mechanics, École Polytechnique, Palaiseau, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Lafaurie-Janvore
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR 7646, Department of Mechanics, École Polytechnique, Palaiseau, France.
authors.forename: Avin
authors.initials: A
authors.lastname: Babataheri
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR 7646, Department of Mechanics, École Polytechnique, Palaiseau, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: de Langre
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR 7646, Department of Mechanics, École Polytechnique, Palaiseau, France.
authors.forename: Abdul I
authors.initials: AI
authors.lastname: Barakat
authors.affiliation: Hydrodynamics Laboratory, CNRS UMR 7646, Department of Mechanics, École Polytechnique, Palaiseau, France. Electronic address: julien.husson@ladhyx.polytechnique.fr.
authors.forename: Julien
authors.initials: J
authors.lastname: Husson
mesh_terms: D008841:Actin Cytoskeleton / Q000378:metabolism; D000818:Animals; D001696:Biomechanical Phenomena; D002417:Cattle; D002462:Cell Membrane / Q000378:metabolism*; D019245:Compressive Strength; D042783:Endothelial Cells / Q000166:cytology; D017276:Friction; D055616:Microtechnology; D013314:Stress, Mechanical