title: High-speed phase-shifting common-path quantitative phase imaging with a piezoelectric actuator.
abstract: We present a phase-shifting quantitative phase imaging technique providing high temporal and spatial phase stability and high acquisition speed. A piezoelectric microfabricated phase modulator allows tunable modulation frequencies up to the kHz range. After assessing the quantitative phase accuracy with technical samples, we demonstrate the high acquisition rate while monitoring cellular processes at temporal scales ranging from milliseconds to hours.
authors.affiliation: Laboratoire d'Optique Biomédicale, École Polytechnique Fédérale de Lausanne, Station 17, 1015 Lausanne, Switzerland.
authors.forename: Séverine
authors.initials: S
authors.lastname: Coquoz
authors.affiliation: Laboratoire d'Optique Biomédicale, École Polytechnique Fédérale de Lausanne, Station 17, 1015 Lausanne, Switzerland.
authors.forename: Amir
authors.initials: A
authors.lastname: Nahas
authors.affiliation: Laboratoire d'Optique Biomédicale, École Polytechnique Fédérale de Lausanne, Station 17, 1015 Lausanne, Switzerland.
authors.forename: Miguel
authors.initials: M
authors.lastname: Sison
authors.affiliation: Laboratoire d'Optique Biomédicale, École Polytechnique Fédérale de Lausanne, Station 17, 1015 Lausanne, Switzerland.
authors.forename: Antonio
authors.initials: A
authors.lastname: Lopez
authors.affiliation: Laboratoire d'Optique Biomédicale, École Polytechnique Fédérale de Lausanne, Station 17, 1015 Lausanne, Switzerland.
authors.forename: Theo
authors.initials: T
authors.lastname: Lasser
mesh_terms: D003584:Cytological Techniques / Q000379:methods*; D004023:Dictyostelium; D004867:Equipment Design; D004926:Escherichia coli; D006367:HeLa Cells; D006801:Humans; D007368:Interferometry / Q000295:instrumentation / Q000379:methods*; D018715:Microscopy, Video; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods*