title: Catecholamine-Stimulated Growth ofAeromonas hydrophilaRequires the TonB2 Energy Transduction System but Is Independent of the Amonabactin Siderophore.
abstract: The growth-stimulating effects of catecholamine stress hormones have been demonstrated in many pathogens. However, catecholamine-induced growth and its underlying mechanisms remain poorly understood inAeromonas hydrophila. The present study sought to demonstrate that norepinephrine (NE), epinephrine (Epi), dopamine (Dopa), and L-dopa stimulate the growth ofA. hydrophilain iron-restricted media containing serum. NE exhibited the strongest growth stimulation, which could be blocked by adrenergic antagonists. Furthermore, it was demonstrated that NE could sequester iron from transferrin, thereby providing a more accessible iron source for utilization byA. hydrophila. The deletion of theamoAgene associated with amonabactin synthesis revealed that the amonabactin siderophore is not required for NE-stimulated growth. However, the deletion of the TonB2 energy transduction system resulted in the loss of growth promotion by NE, indicating that a specific TonB-dependent outer membrane receptor might be involved in the transport of iron from transferrin. Collectively, our data show that catecholamine sensing promotes the growth ofA. hydrophilain a manner that is dependent on the TonB2 energy transduction system.
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors.forename: Yuhao
authors.initials: Y
authors.lastname: Dong
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural UniversityNanjing, China; Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base, Institute of Food Safety, Jiangsu Academy of Agricultural SciencesNanjing, China.
authors.forename: Maoda
authors.initials: M
authors.lastname: Pang
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors.forename: Hechao
authors.initials: H
authors.lastname: Du
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors.forename: Nannan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors.forename: Furqan
authors.initials: F
authors.lastname: Awan
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors.forename: Chengping
authors.initials: C
authors.lastname: Lu
authors.affiliation: Department of Preventive Veterinary, College of Veterinary Medicine, Nanjing Agricultural University Nanjing, China.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Liu
mesh_terms: D018674:Adrenergic Antagonists; D016980:Aeromonas hydrophila / Q000187:drug effects* / Q000254:growth & development* / Q000378:metabolism; D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism; D018441:Biofilms / Q000254:growth & development; D002352:Carrier Proteins; D002395:Catecholamines / Q000494:pharmacology*; D002460:Cell Line; D004269:DNA, Bacterial / Q000235:genetics; D004298:Dopamine / Q000494:pharmacology; D004837:Epinephrine / Q000494:pharmacology; D004926:Escherichia coli / Q000235:genetics; D005260:Female; D005798:Genes, Bacterial; D007501:Iron / Q000378:metabolism; D007980:Levodopa / Q000494:pharmacology; D051379:Mice; D008813:Mice, Inbred ICR; D009638:Norepinephrine / Q000494:pharmacology; D009842:Oligopeptides / Q000378:metabolism; D017384:Sequence Deletion; D017262:Siderophores / Q000378:metabolism*; D015398:Signal Transduction; D013315:Stress, Psychological; D014168:Transferrin / Q000737:chemistry / Q000378:metabolism
chemical_list: D018674:Adrenergic Antagonists; D001425:Bacterial Outer Membrane Proteins; D002352:Carrier Proteins; D002395:Catecholamines; D004269:DNA, Bacterial; D009842:Oligopeptides; D017262:Siderophores; D014168:Transferrin; D007980:Levodopa; D007501:Iron; D004298:Dopamine; D009638:Norepinephrine; D004837:Epinephrine
keywords: Aeromonas hydrophila; TonB2 energy transduction system; amonabactin; catecholamine; stress