title: Interaction of Prions Causes Heritable Traits in Saccharomyces cerevisiae.
abstract: The concept of "protein-based inheritance" defines prions as epigenetic determinants that cause several heritable traits in eukaryotic microorganisms, such as Saccharomyces cerevisiae and Podospora anserina. Previously, we discovered a non-chromosomal factor, [NSI+], which possesses the main features of yeast prions, including cytoplasmic infectivity, reversible curability, dominance, and non-Mendelian inheritance in meiosis. This factor causes omnipotent suppression of nonsense mutations in strains of S. cerevisiae bearing a deleted or modified Sup35 N-terminal domain. In this work, we identified protein determinants of [NSI+] using an original method of proteomic screening for prions. The suppression of nonsense mutations in [NSI+] strains is determined by the interaction between [SWI+] and [PIN+] prions. Using genetic and biochemical methods, we showed that [SWI+] is the key determinant of this nonsense suppression, whereas [PIN+] does not cause nonsense suppression by itself but strongly enhances the effect of [SWI+]. We demonstrated that interaction of [SWI+] and [PIN+] causes inactivation of SUP45 gene that leads to nonsense suppression. Our data show that prion interactions may cause heritable traits in Saccharomyces cerevisiae.
authors.affiliation: St. Petersburg State University, Department of Genetics and Biotechnology, 199034 St. Petersburg, Russian Federation.|Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences, 199034 St. Petersburg, Russian Federation.
authors.forename: Anton A
authors.identifier: http://orcid.org/0000-0002-8338-3494
authors.initials: AA
authors.lastname: Nizhnikov
authors.affiliation: St. Petersburg State University, Department of Genetics and Biotechnology, 199034 St. Petersburg, Russian Federation.|Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences, 199034 St. Petersburg, Russian Federation.
authors.forename: Tatyana A
authors.initials: TA
authors.lastname: Ryzhova
authors.affiliation: St. Petersburg State University, Research Park, Research Resource Center "Molecular and Cell Technologies", St. Petersburg, Russian Federation.
authors.forename: Kirill V
authors.identifier: http://orcid.org/0000-0002-8027-5930
authors.initials: KV
authors.lastname: Volkov
authors.affiliation: St. Petersburg State University, Department of Genetics and Biotechnology, 199034 St. Petersburg, Russian Federation.|Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences, 199034 St. Petersburg, Russian Federation.
authors.forename: Sergey P
authors.identifier: http://orcid.org/0000-0001-8859-164X
authors.initials: SP
authors.lastname: Zadorsky
authors.affiliation: St. Petersburg State University, Department of Genetics and Biotechnology, 199034 St. Petersburg, Russian Federation.|Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences, 199034 St. Petersburg, Russian Federation.
authors.forename: Julia V
authors.identifier: http://orcid.org/0000-0002-7825-273X
authors.initials: JV
authors.lastname: Sopova
authors.affiliation: St. Petersburg State University, Department of Genetics and Biotechnology, 199034 St. Petersburg, Russian Federation.|Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences, 199034 St. Petersburg, Russian Federation.
authors.forename: Sergey G
authors.initials: SG
authors.lastname: Inge-Vechtomov
authors.affiliation: St. Petersburg State University, Department of Genetics and Biotechnology, 199034 St. Petersburg, Russian Federation.|Vavilov Institute of General Genetics, St. Petersburg Branch, Russian Academy of Sciences, 199034 St. Petersburg, Russian Federation.
authors.forename: Alexey P
authors.initials: AP
authors.lastname: Galkin
mesh_terms: D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics; D018389:Codon, Nonsense; D004268:DNA-Binding Proteins / Q000235:genetics; D005690:Galactose / Q000235:genetics; D008540:Meiosis / Q000235:genetics*; D008856:Microscopy, Fluorescence; D010454:Peptide Termination Factors / Q000235:genetics* / Q000378:metabolism; D010957:Plasmids / Q000235:genetics; D011328:Prions / Q000235:genetics*; D040901:Proteomics; D012441:Saccharomyces cerevisiae / Q000235:genetics; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics* / Q000378:metabolism; D017384:Sequence Deletion; D014157:Transcription Factors / Q000235:genetics
chemical_list: D002868:Chromosomal Proteins, Non-Histone; D018389:Codon, Nonsense; D004268:DNA-Binding Proteins; C574300:Nsi1 protein, S cerevisiae; D010454:Peptide Termination Factors; D011328:Prions; C068068:SUP35 protein, S cerevisiae; C080775:SUP45 protein, S cerevisiae; C078562:SWI1 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins; D014157:Transcription Factors; D005690:Galactose