title: Neuroprotective effect of hypobaric hypoxic postconditioning is accompanied by dna protection and lipid peroxidation changes in rat hippocampus.
abstract: The present study was performed to explore the effect of severe hypobaric hypoxia (180Torr, 3h) and severe hypoxia followed by hypoxic postconditioning (360Torr, 2h, 3 episodes) on DNA fragmentation and dynamics of lipid peroxidation products in rat hippocampus. The severe hypoxia induced intense DNA fragmentation in the hippocampus. A persistent decrease of thiobarbituric acid reactive substances in the hippocampus was also detected in response to severe hypoxia while the levels of Schiff bases did not significantly change. The postconditioning prevented severe hypoxia-induced DNA fragmentation, returned the levels of thiobarbituric acid reactive substances to the baseline and decreased the levels of Schiff bases. These findings indicate that the neuroprotective effect of hypoxic postconditioning on hippocampal neurons detected as suppression of hypoxia-induced DNA fragmentation is accompanied by the changes in lipid peroxidation processes.
authors.affiliation: Laboratory of Regulation of Brain Neuronal Functions, Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb. 6, 199034 Saint-Petersburg, Russian Federation; Department of Biochemistry, Faculty of Biology, Saint-Petersburg State University, Universitetskaya emb. 7-9, 199034 Saint- Petersburg, Russian Federation. Electronic address: vov210292@yandex.ru.
authors.forename: Oleg
authors.initials: O
authors.lastname: Vetrovoy
authors.affiliation: Laboratory of Regulation of Brain Neuronal Functions, Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb. 6, 199034 Saint-Petersburg, Russian Federation.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Tulkova
authors.affiliation: Laboratory of Regulation of Brain Neuronal Functions, Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb. 6, 199034 Saint-Petersburg, Russian Federation; Department of Biochemistry, Faculty of Biology, Saint-Petersburg State University, Universitetskaya emb. 7-9, 199034 Saint- Petersburg, Russian Federation.
authors.forename: Ksenia
authors.initials: K
authors.lastname: Sarieva
authors.affiliation: Department of Biochemistry, Faculty of Biology, Saint-Petersburg State University, Universitetskaya emb. 7-9, 199034 Saint- Petersburg, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Kotryahova
authors.affiliation: Laboratory of Regulation of Brain Neuronal Functions, Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb. 6, 199034 Saint-Petersburg, Russian Federation.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Zenko
authors.affiliation: Laboratory of Regulation of Brain Neuronal Functions, Pavlov Institute of Physiology, Russian Academy of Sciences, Makarova emb. 6, 199034 Saint-Petersburg, Russian Federation.
authors.forename: Elena
authors.initials: E
authors.lastname: Rybnikova
mesh_terms: D000818:Animals; D002450:Cell Communication / Q000187:drug effects; D004247:DNA / Q000235:genetics*; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D000860:Hypoxia / Q000378:metabolism*; D057775:Ischemic Postconditioning / Q000379:methods; D015227:Lipid Peroxidation / Q000187:drug effects*; D008297:Male; D019579:Neocortex / Q000187:drug effects; D009474:Neurons / Q000187:drug effects* / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D017208:Rats, Wistar
chemical_list: D018696:Neuroprotective Agents; D004247:DNA
keywords: DNA fragmentation; Hippocampus; Lipid peroxidation; Mild hypobaric hypoxia postconditioning; Neuroprotection.; Severe hypobaric hypoxi a