title: Short-term Exposure to 50-Hz Electromagnetic Field and Alterations inNQO1andNQO2Expression in MCF-7 Cells.
abstract: OBJECTIVE
  Extremely low-frequency electromagnetic fields (ELF-EMFs) have some genotoxic effects and it may alter the mRNA levels of antioxidant genes. The NAD(P)H: quinone oxidoreductase-1 (NQO1) andNQO2are ubiquitously expressed. Considering that there is no published data on the effect(s) of ELF-EMF (50-Hz) exposure and expression levels ofNQO1andNQO2in the human MCF-7 cells, the present study was carried out.
  
  
  METHODS
  The ELF-EMF (0.25 and 0.50 mT) exposure patterns were: 5 min field-on/5 min filed-off, 15 min field-on/15 min field-off, and 30 min field-on continuously. In all exposure conditions, total exposure time were 30 minutes. The RNA extraction was done at two times; immediately post exposure and two hours post exposure. The effect of ELF-EMF on gene expression was assessed by real-time PCR.
  
  
  RESULTS
  TheNQO1mRNA level (at 0h) decreased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.25 mT EMF when compared with the unexposed cells. TheNQO2mRNA level (at 0h and 2h) increased in the cells exposed to 5 min field-on/5 min filed-off condition at 0.50 mT EMF when compared with the unexposed cells.
  
  
  CONCLUSIONS
  Alterations in theNQO1andNQO2mRNA levels seem at the "5 min field-on/5 min field-off" condition.
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran.
authors.forename: Hamideh
authors.initials: H
authors.lastname: Mahmoudinasab
authors.affiliation: Department of Biology, College of Sciences, Shiraz University, Shiraz 71467-13565, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Saadat
keywords: Electromagnetic field; Gene expression; MCF-7; NQO1; NQO2