title: Clinical and subclinical bovine leukemia virus infection in a dairy cattle herd in Zambia.
abstract: Bovine leukemia virus (BLV) causes enzootic bovine leucosis (EBL) and is responsible for substantial economic losses in cattle globally. However, information in Africa on the disease is limited. Here, based on clinical, hematological, pathological and molecular analyses, two clinical cases of EBL were confirmed in a dairy cattle herd in Zambia. In contrast, proviral DNA was detected by PCR in five apparently healthy cows from the same herd, suggesting subclinical BLV infection. Phylogenetic analysis of the env gene showed that the identified BLV clustered with Eurasian genotype 4 strains. This is the first report of confirmed EBL in Zambia.
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia. pandeygs@gmail.com.
authors.forename: Girja S
authors.initials: GS
authors.lastname: Pandey
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Edgar
authors.initials: E
authors.lastname: Simulundu
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Danstan
authors.initials: D
authors.lastname: Mwiinga
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Kenny L
authors.initials: KL
authors.lastname: Samui
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Mweene
authors.affiliation: Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kajihara
authors.affiliation: Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Alfred
authors.initials: A
authors.lastname: Mangani
authors.affiliation: Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Racheal
authors.initials: R
authors.lastname: Mwenda
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.
authors.forename: Joseph
authors.initials: J
authors.lastname: Ndebe
authors.affiliation: Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Konnai
authors.affiliation: Department of Disease Control, School of Veterinary Medicine, University of Zambia, PO Box 32379, Lusaka, Zambia.|Division of Global Epidemiology, Hokkaido University Research Center for Zoonosis Control, Sapporo, Japan.|Global Institution for Collaborative Research and Education, Hokkaido University, Sapporo, Japan.
authors.forename: Ayato
authors.initials: A
authors.lastname: Takada
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D016583:Enzootic Bovine Leukosis / Q000821:virology*; D005260:Female; D005838:Genotype; D001909:Leukemia Virus, Bovine / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D008297:Male; D008969:Molecular Sequence Data; D010802:Phylogeny; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics; D015024:Zambia
chemical_list: D014764:Viral Proteins