title: Preparation and evaluation of a hair wax containing propolis andEruca sativaseed oil for hair growth.
abstract: BACKGROUND
  Hair growth as a key consumer objective has important role in the hair care products researches. This study was aimed to investigate the effect of a hair wax containing propolis, a resinous mixture produced by honeybees inEruca sativaseed oil base on hair growth.
  
  
  METHODS
  The hair wax was designed and formulated compared with marketed brand hair wax and evaluated for pharmaceutical parameters including pH, homogeneity, consistency, spread ability,in vitrodrug release, and stability. After selection of the best formulation containing 10% ethanolic extract of propolis and 10%E. sativaseed oil, the hair growth potential was evaluated by application of 1 g hair wax daily on 4 cm2area of dorsal side of Wistar rats and compared with controls and standard medication (1 ml of 2% minoxidil). After 30 days treatment, the length and weight of hairs and percentage of hair follicles in different phases of growth in skin biopsies were assessed.
  
  
  RESULTS
  The selected hair wax formulation was stable and easy to wash. The formulation significantly increased hair length on 10th, 20th, and 30thday compared control group (5.8 ± 0.3 vs. 2.6 ± 0.4, 11.4 ± 0.6 vs. 5.8 ± 0.4, and 17.5 ± 0.5 vs. 12.7 ± 0.4 mm, respectively) and also the weight of newly grown hairs on 30thday (0.056 ± 0.006 vs. 0.043 ± 0.005). It improved hair follicles percentages in anagen phase without any sensitivity reaction.
  
  
  CONCLUSIONS
  The results of this study suggest that the formulated hair wax containing of propolis andE. sativaseed oil could have significant effect on promoting hair growth.
authors.affiliation: Department of Pharmaceutics, Novel Drug Delivery Systems Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mohammad-Ali
authors.initials: MA
authors.lastname: Shatalebi
authors.affiliation: Department of Pharmacology and Toxicology, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Safaeian
authors.affiliation: Department of Clinical Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Azar
authors.initials: A
authors.lastname: Baradaran
authors.affiliation: Department of Pharmaceutics, Novel Drug Delivery Systems Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
authors.forename: Mozhde
authors.initials: M
authors.lastname: Alamdarian
keywords: Eruca sativa Mill.; hair growth; hair wax; propolis