title: SDF-1/CXCL12 and CXCR4 gene variants, and elevated serum SDF-1 levels are associated with preeclampsia.
abstract: OBJECTIVE
  We aimed to compare the frequencies of stromal cell-derived factor-1 (SDF-1) 3'A and CXCR4 single-nucleotide polymorphisms (SNPs) and serum SDF-1 levels in patients with preeclampsia (PE).
  
  
  METHODS
  In total, 89 women with PE and 89 control women were included in the study. Genotyping was done by polymerase chain reaction-restriction fragment length polymorphism method. Enzyme-linked immunosorbent assay method was used to measure serum SDF-1 level.
  
  
  RESULTS
  For SDF-1 3'A SNP, the frequency of GA genotype, total number of GA and AA genotypes, and the A allele frequency was higher in PE patients than controls (p = 0.04, 0.023, and 0.029, respectively). For CXCR4 SNP, the frequency of CT genotype, total number of CT and TT genotypes, and the T allele frequency were higher in PE patients than controls (p = 0.04, 0.006, and 0.005, respectively). SDF-1 serum level was detected higher in preeclamptic women compared with controls (p = 0.001). In PE patients, there was no significant association between serum SDF-1 levels and genotypes of SDF-1 3'A SNP. SDF-1 level was significantly higher in patients bearing CXCR4 CT genotype than CC genotype (p = 0.001). Furthermore, SDF-1 levels in patients bearing CT+TT genotype were found higher than that of patients with CC genotypes (p = 0.001).
  
  
  CONCLUSIONS
  Results of our study suggest that SDF-1 3'A and CXCR4 polymorphisms and elevated serum SDF-1 levels may have a role in the development of PE.
authors.affiliation: a Department of Obstetrics and Gynecology , Cumhuriyet University School of Medicine , Sivas , Turkey.
authors.forename: Savas
authors.initials: S
authors.lastname: Karakus
authors.affiliation: b Department of Nutrition and Dietetics , Cumhuriyet University School of Health Sciences , Sivas , Turkey.
authors.forename: Binnur
authors.initials: B
authors.lastname: Bagci
authors.affiliation: c Department of Medical Genetics , Cumhuriyet University School of Medicine , Sivas , Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Bagci
authors.affiliation: d Department of Biochemistry , Cumhuriyet University School of Medicine , Sivas , Turkey.
authors.forename: Enver
authors.initials: E
authors.lastname: Sancakdar
authors.affiliation: a Department of Obstetrics and Gynecology , Cumhuriyet University School of Medicine , Sivas , Turkey.
authors.forename: Caglar
authors.initials: C
authors.lastname: Yildiz
authors.affiliation: a Department of Obstetrics and Gynecology , Cumhuriyet University School of Medicine , Sivas , Turkey.
authors.forename: Ozlem
authors.initials: O
authors.lastname: Akkar
authors.affiliation: a Department of Obstetrics and Gynecology , Cumhuriyet University School of Medicine , Sivas , Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Cetin
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D054377:Chemokine CXCL12 / Q000097:blood / Q000235:genetics*; D005260:Female; D006801:Humans; D020641:Polymorphism, Single Nucleotide; D011225:Pre-Eclampsia / Q000097:blood / Q000235:genetics*; D011247:Pregnancy; D019718:Receptors, CXCR4 / Q000235:genetics*; D055815:Young Adult
chemical_list: D015415:Biomarkers; C515255:CXCL12 protein, human; C527276:CXCR4 protein, human; D054377:Chemokine CXCL12; D019718:Receptors, CXCR4
keywords: CXCL12 gene; CXCR4 gene; SDF-1; polymorphism; preeclampsia