title: Hypoxia induced Bcl-2/Twist1 complex promotes tumor cell invasion in oral squamous cell carcinoma.
abstract: Bcl-2 and Twist1 can be coactivated by hypoxia in hepatocellular carcinoma to promote tumor cell metastasis and vasculogenic mimicry, but their function in oral squamous cell carcinoma (OSCC) remains undefined. We employed a cohort of 82 cases of OSCC samples to examine the coexpression of Bcl-2 and Twist1 by immunohistochemistry and demonstrate the interaction between Bcl-2 and Twist1 by coimmunoprecipitation. Bcl-2 and Twist1 overexpression was associated with a poor pathological grade and tumor prognosis, and the two factors functions as a complex. Knocking down Bcl-2/Twist1 inhibited cell migration, decreased cell invasion and inversed cell epithelial-mesenchymal transition (EMT) procession. An animal model derived from the Tca8113 cell line was used to further validate the role of Bcl-2/Twist1 depletion in suppressing tumor EMT and growth. In conclusion, Bcl-2/Twist1 complex can be treated as a potential therapeutic target for OSCC.
authors.affiliation: Department of Maxillofacial and E.N.T Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center for Cancer, Key Laboratory of Cancer Preventionand Therapy, Tianjin 300060, China.
authors.forename: Yuansheng
authors.initials: Y
authors.lastname: Duan
authors.affiliation: Department of Maxillofacial and E.N.T Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center for Cancer, Key Laboratory of Cancer Preventionand Therapy, Tianjin 300060, China.
authors.forename: Qinghua
authors.initials: Q
authors.lastname: He
authors.affiliation: Department of Maxillofacial and E.N.T Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center for Cancer, Key Laboratory of Cancer Preventionand Therapy, Tianjin 300060, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Yue
authors.affiliation: Department of Maxillofacial and E.N.T Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center for Cancer, Key Laboratory of Cancer Preventionand Therapy, Tianjin 300060, China.
authors.forename: Haishan
authors.initials: H
authors.lastname: Si
authors.affiliation: Department of Maxillofacial and E.N.T Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center for Cancer, Key Laboratory of Cancer Preventionand Therapy, Tianjin 300060, China.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Maxillofacial and E.N.T Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center for Cancer, Key Laboratory of Cancer Preventionand Therapy, Tianjin 300060, China.
authors.forename: Xuan
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Maxillofacial and E.N.T Oncology, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, China.|National Clinical Research Center for Cancer, Key Laboratory of Cancer Preventionand Therapy, Tianjin 300060, China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement; D058750:Epithelial-Mesenchymal Transition; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006258:Head and Neck Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008297:Male; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D008875:Middle Aged; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D060787:Neoplasm Grading; D009361:Neoplasm Invasiveness; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism*; D019253:Proto-Oncogene Proteins c-bcl-2 / Q000235:genetics / Q000378:metabolism*; D034622:RNA Interference; D015398:Signal Transduction; D000077195:Squamous Cell Carcinoma of Head and Neck; D014162:Transfection; D000072258:Tumor Hypoxia; D059016:Tumor Microenvironment; D051879:Twist-Related Protein 1 / Q000235:genetics / Q000378:metabolism*
chemical_list: C000595707:BCL2 protein, human; D009687:Nuclear Proteins; D019253:Proto-Oncogene Proteins c-bcl-2; C103173:TWIST1 protein, human; D051879:Twist-Related Protein 1
keywords: Bcl-2; EMT; Twist1; oral squamous cell carcinoma; tumor invasion