title: Multi-InDel Analysis for Ancestry Inference of Sub-Populations in China.
abstract: Ancestry inference is of great interest in diverse areas of scientific researches, including the forensic biology, medical genetics and anthropology. Various methods have been published for distinguishing populations. However, few reports refer to sub-populations (like ethnic groups) within Asian populations for the limitation of markers. Several InDel loci located very tightly in physical positions were treated as one marker by us, which is multi-InDel. The multi-InDel shows potential as Ancestry Inference Marker (AIM). In this study, we performed a genome-wide scan for multi-InDels as AIM. After examining the FSTdistributions in the 1000 Genomes Database, 12 candidates were selected and validated for eastern Asian populations. A multiplexed assay was developed as a panel to genotype 12 multi-InDel markers simultaneously. Ancestry component analysis with STRUCTURE and principal component analysis (PCA) were employed to estimate its capability for ancestry inference. Furthermore, ancestry assignments of trial individuals were conducted. It proved to be very effective when 210 samples from Han and Tibetan individuals in China were tested. The panel consisting of multi-InDel markers exhibited considerable potency in ancestry inference, and was suggested to be applied in forensic practices and genetic population studies.
authors.affiliation: Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, P.R. China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Sun
authors.affiliation: Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Ye
authors.affiliation: Laboratory of Infection and Immunity, School of Basic Medical Sciences, West China Center of Medical Science, Sichuan University, Chengdu P.R. China.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: Institute of Forensic Medicine, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, P.R. China.
authors.forename: Yiping
authors.initials: Y
authors.lastname: Hou
mesh_terms: D044466:Asian People / Q000208:ethnology* / Q000235:genetics*; D002681:China / Q000208:ethnology; D030561:Databases, Nucleic Acid; D005260:Female; D015894:Genome, Human; D006801:Humans; D054643:INDEL Mutation; D008297:Male