title: IL-17B is elevated in patients with pneumonia and mediates IL-8 production in bronchial epithelial cells.
abstract: The role of IL-17B in regulating pulmonary immunity and inflammation is unknown. In this study, we found that IL-17B concentrations were significantly elevated in adult and paediatric patients with community-acquired pneumonia relative to their corresponding healthy adult and paediatric controls. The increased concentrations of IL-17B significantly and positively correlated with chemokine IL-8 concentrations in clinical pneumonia. In vitro studies demonstrated that IL-17B could induce gene and protein expression of IL-8 in human bronchial epithelial cells, but not lung fibroblasts, which was regulated by the activation of Akt, p38 mitogen-activated protein kinase (MAPK), extracellular signal-regulated kinase (ERK) and nuclear factor-kappaB (NF-κB) signaling pathways. In vivo studies further showed that increased IL-17B levels significantly and positively correlated with IL-8 concentrations in experimental pneumonia. In conclusion, human pneumonia was associated with enhanced release of IL-17B, which might regulate pulmonary immunity and inflammation through the induction of IL-8 in bronchial epithelial cells.
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China; Key Laboratory of Diagnostic Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Medical Examination Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ren
authors.affiliation: Clinical Laboratories Center, Children's Hospital, Chongqing Medical University, Chongqing, China.
authors.forename: Dapeng
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Lin
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
authors.forename: Shifeng
authors.initials: S
authors.lastname: Huang
authors.affiliation: Key Laboratory of Diagnostic Medicine designated by the Ministry of Education, Chongqing Medical University, Chongqing, China. Electronic address: yibingyin723@163.com.
authors.forename: Yibing
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Department of Laboratory Medicine, The First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Electronic address: 870049585@qq.com.
authors.forename: Ju
authors.initials: J
authors.lastname: Cao
mesh_terms: D000328:Adult; D000368:Aged; D000818:Animals; D001980:Bronchi / Q000378:metabolism*; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000378:metabolism*; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005260:Female; D005347:Fibroblasts / Q000378:metabolism; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D016209:Interleukin-8 / Q000378:metabolism*; D008168:Lung / Q000378:metabolism; D020935:MAP Kinase Signaling System / Q000502:physiology; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D008875:Middle Aged; D016328:NF-kappa B / Q000378:metabolism; D011014:Pneumonia / Q000378:metabolism*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism
chemical_list: D020381:Interleukin-17; D016209:Interleukin-8; D016328:NF-kappa B; D048049:Extracellular Signal-Regulated MAP Kinases; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Human lung tissue cells; Il-17B; Il-8; Infection; Pulmonary immunity