title: A discretized model for enzymatic hydrolysis of cellulose in a fed-batch process.
abstract: In the enzymatic hydrolysis of cellulose, several phenomena have been proposed to cause a decrease in the reaction rate with increasing conversion. The importance of each phenomenon is difficult to distinguish from batch hydrolysis data. Thus, kinetic models for the enzymatic hydrolysis of cellulose often suffer from poor parameter identifiability. This work presents a model that is applicable to fed-batch hydrolysis by discretizing the substrate based on the feeding time. Different scenarios are tested to explain the observed decrease in reaction rate with increasing conversion, and comprehensive assessment of the parameter sensitivities is carried out. The proposed model performed well in the broad range of experimental conditions used in this study and when compared to literature data. Furthermore, the use of data from fed-batch experiments and discretization of the model substrate to populations was found to be very informative when assessing the importance of the rate-decreasing phenomena in the model.
authors.affiliation: Chemical Process Engineering, Faculty of Technology, University of Oulu, P.O. Box 4300, FI-90014 Oulun yliopisto, Finland. Electronic address: petri.tervasmaki@oulu.fi.
authors.forename: Petri
authors.initials: P
authors.lastname: Tervasmäki
authors.affiliation: Chemical Process Engineering, Faculty of Technology, University of Oulu, P.O. Box 4300, FI-90014 Oulun yliopisto, Finland.
authors.forename: Ville
authors.initials: V
authors.lastname: Sotaniemi
authors.affiliation: Chemical Process Engineering, Faculty of Technology, University of Oulu, P.O. Box 4300, FI-90014 Oulun yliopisto, Finland.
authors.forename: Jani
authors.initials: J
authors.lastname: Kangas
authors.affiliation: Chemical Process Engineering, Faculty of Technology, University of Oulu, P.O. Box 4300, FI-90014 Oulun yliopisto, Finland.
authors.forename: Sanna
authors.initials: S
authors.lastname: Taskila
authors.affiliation: Department of Biotechnology and Chemical Technology, School of Chemical Technology, Aalto University, P.O. Box 16100, 00076, Aalto, Finland.
authors.forename: Heikki
authors.initials: H
authors.lastname: Ojamo
authors.affiliation: Chemical Process Engineering, Faculty of Technology, University of Oulu, P.O. Box 4300, FI-90014 Oulun yliopisto, Finland.
authors.forename: Juha
authors.initials: J
authors.lastname: Tanskanen
mesh_terms: D061249:Batch Cell Culture Techniques; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D006868:Hydrolysis; D007700:Kinetics; D008962:Models, Theoretical
chemical_list: D002482:Cellulose; D002480:Cellulase
keywords: Cellulose; Enzymatic hydrolysis; Fed-batch; Kinetic model