title: Using polyvinylpyrrolidone to enhance the enzymatic hydrolysis of lignocelluloses by reducing the cellulase non-productive adsorption on lignin.
abstract: Polyvinylpyrrolidone (PVP) is an antifouling polymer to resist the adsorption of protein on solid surface. Effects of PVP on the enzymatic hydrolysis of pretreated lignocelluloses and its mechanism were studied. Adding 1g/L of PVP8000, the enzymatic digestibility of eucalyptus pretreated by dilute acid (Eu-DA) was increased from 28.9% to 73.4%, which is stronger than the classic additives, such as PEG, Tween and bovine serum albumin. Compared with PEG4600, the adsorption of PVP8000 on lignin was larger, and the adsorption layer was more stable and hydrophilic. Therefore, PVP8000 reduced 73.1% of the cellulase non-productive adsorption on lignin and enhanced the enzymatic hydrolysis of lignocelluloses greatly.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Cai
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. Electronic address: xueqingqiu66@163.com.
authors.forename: Xueqing
authors.initials: X
authors.lastname: Qiu
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Meijun
authors.initials: M
authors.lastname: Zeng
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Meilu
authors.initials: M
authors.lastname: Lin
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Xuliang
authors.initials: X
authors.lastname: Lin
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China; State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China. Electronic address: cehmlou@scut.edu.cn.
authors.forename: Hongming
authors.initials: H
authors.lastname: Lou
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Xuejuan
authors.initials: X
authors.lastname: Zhan
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Yuxia
authors.initials: Y
authors.lastname: Pang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Jinhao
authors.initials: J
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China.
authors.forename: Lingshan
authors.initials: L
authors.lastname: Xie
mesh_terms: D000327:Adsorption; D002480:Cellulase / Q000737:chemistry; D005052:Eucalyptus / Q000737:chemistry / Q000378:metabolism; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D011205:Povidone / Q000737:chemistry*
chemical_list: C036909:lignocellulose; D008031:Lignin; D002480:Cellulase; D011205:Povidone
keywords: Cellulase; Enzymatic hydrolysis; Lignocellulose; Non-productive adsorption; Polyvinylpyrrolidone