title: Zoonotic potential of Enterocytozoon genotypes in humans and pigs in Thailand.
abstract: Enterocytozoon bieneusi is an opportunistic intestinal pathogen infecting humans and a variety of animals. Its mode of transmission and zoonotic potential are not completely understood. E. bieneusi has been frequently identified in pigs. The objective of our study was to investigate E. bieneusi in pigs and humans in Western and Central Thailand to determine its presence, genetic diversity, and zoonotic potential. A total of 277 human and 210 pig faecal samples were collected and analysed. E. bieneusi was found in 5.4% and 28.1% of human and pig samples, respectively, by nested PCR. Genotyping based on the internal transcribed spacer regions of the small subunit ribosomal RNA demonstrated three known genotypes (D, H, PigEb10) and eight novel genotypes (TMH1-8) in humans, and five known genotypes (D, EbpA, EbpC, H, O) and 11 novel genotypes (TMP1-11) in pigs. All known genotypes identified in humans and pigs had zoonotic potential. Further studies are needed to evaluate zoonotic risk of novel genotypes, as pigs may play an important role in the transmission of E. bieneusi.
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Rapeepun
authors.initials: R
authors.lastname: Prasertbun
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Hirotake
authors.initials: H
authors.lastname: Mori
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Ai-Rada
authors.initials: AR
authors.lastname: Pintong
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Suparut
authors.initials: S
authors.lastname: Sanyanusin
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Supaluk
authors.initials: S
authors.lastname: Popruk
authors.affiliation: Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Chalit
authors.initials: C
authors.lastname: Komalamisra
authors.affiliation: Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand; The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Tanasak
authors.initials: T
authors.lastname: Changbunjong
authors.affiliation: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand; Department of Clinical Sciences and Public Health Medicine, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom, Thailand.
authors.forename: Ruangrat
authors.initials: R
authors.lastname: Buddhirongawatr
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.
authors.forename: Yaowalark
authors.initials: Y
authors.lastname: Sukthana
authors.affiliation: Department of Protozoology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand. Electronic address: aongart.mah@mahidol.ac.th.
authors.forename: Aongart
authors.initials: A
authors.lastname: Mahittikorn
mesh_terms: D000818:Animals; D021903:DNA, Ribosomal Spacer / Q000235:genetics; D021862:Enterocytozoon / Q000235:genetics* / Q000472:pathogenicity; D005243:Feces / Q000469:parasitology; D014644:Genetic Variation; D005838:Genotype; D006801:Humans; D016881:Microsporidiosis / Q000469:parasitology* / Q000635:transmission; D013552:Swine; D013553:Swine Diseases / Q000469:parasitology* / Q000635:transmission; D013785:Thailand; D015047:Zoonoses / Q000469:parasitology* / Q000635:transmission
chemical_list: D021903:DNA, Ribosomal Spacer
keywords: Enterocytozoon; Human; Pig; Thailand; Zoonosis