title: Polyaspartamide-based graft copolymers encapsulating iron oxide nanoparticles for imaging and fluorescence labelling of immune cells.
abstract: Iron oxide nanoparticles (NPs) were encapsulated with polyaspartamide-based graft copolymers to bind and track the immune cells as imaging probes. Mono-disperse iron oxide NPs with a mean diameter of 10.7 nm were synthesized by the thermal decomposition method, and their shape and distribution were measured by electrophoretic light scattering and transmission electron microscopy. To enhance their biocompatibility, interfacial and hydrodynamic stability, and fluorescence detection, biodegradable polysuccinimide (PSI) grafted with several functional groups of octadecylamine (C18), ethanolamine (EA), ethylenediamine (EDA), 4-(N-maleimidomethyl) cyclohexane carboxylic acid N-hydroxysuccinimide ester (SMCC), and fluorescein isothiocyanate (FITC) was coated on the iron oxide NPs. The structure of the C18/EA/SMCC/FITC-g-PSI copolymer was confirmed using1H-NMR and FTIR spectroscopy, and its cell binding ability was investigated by flow cytometry and confocal laser scanning microscopy. The synthesized C18/EA/SMCC/FITC-g-PSI copolymer showed an excellent binding affinity to CD4+ T cells, and was highly biocompatible as the cell viability at the highest polymer concentration of 0.4 mg mL-1was greater than 85 and 75% after 24 and 48 h, respectively, from MTT assay.
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Kyunggi 440-746, Republic of Korea. djkim@skku.edu.
authors.forename: Minsun
authors.identifier: http://orcid.org/0000-0003-2654-4201
authors.initials: M
authors.lastname: Kim
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Kyunggi 440-746, Republic of Korea. djkim@skku.edu.
authors.forename: Seung Won
authors.initials: SW
authors.lastname: Shin
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Kyunggi 440-746, Republic of Korea. djkim@skku.edu.
authors.forename: Cheol Won
authors.initials: CW
authors.lastname: Lim
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Kyunggi 440-746, Republic of Korea. djkim@skku.edu.
authors.forename: Jaeyun
authors.initials: J
authors.lastname: Kim
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Kyunggi 440-746, Republic of Korea. djkim@skku.edu.
authors.forename: Soong Ho
authors.initials: SH
authors.lastname: Um
authors.affiliation: School of Chemical Engineering, Sungkyunkwan University, Suwon, Kyunggi 440-746, Republic of Korea. djkim@skku.edu.
authors.forename: Dukjoon
authors.identifier: http://orcid.org/0000-0001-6187-0737
authors.initials: D
authors.lastname: Kim
mesh_terms: D001224:Aspartic Acid; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D005290:Ferric Compounds / Q000138:chemical synthesis / Q000737:chemistry* / Q000494:pharmacology*; D005453:Fluorescence; D006801:Humans; D018613:Microscopy, Confocal; D053758:Nanoparticles / Q000737:chemistry*; D061848:Optical Imaging; D010316:Particle Size
chemical_list: D005290:Ferric Compounds; C000499:ferric oxide; D001224:Aspartic Acid