title: Sodium Butyrate Ameliorates High-Concentrate Diet-Induced Inflammation in the Rumen Epithelium of Dairy Goats.
abstract: To investigate the effect of sodium butyrate on high-concentrate diet-induced local inflammation of the rumen epithelium, 18 midlactating dairy goats were randomly assigned to 3 groups: a low-concentrate diet group as the control (concentrate:forage = 4:6), a high-concentrate (HC) diet group (concentrate:forage = 6:4), and a sodium butyrate (SB) group (concentrate:forage = 6:4, with 1% SB by weight). The results showed that, with the addition of sodium butyrate, the concentration of lipopolysaccharide (LPS) in rumen fluid (2.62 × 104± 2.90 × 103EU/mL) was significantly lower than that in the HC group (4.03 × 104± 2.77 × 103EU/mL). The protein abundance of pp65, gene expression of proinflammatory cytokines, and activity of myeloperoxidase (MPO) and matrix metalloproteinase (MMP)-2,9 in the rumen epithelium were significantly down-regulated by SB compared with those in the HC group. With sodium butyrate administration, the concentration of NH3-N (19.2 ± 0.890 mM) in the rumen fluid was significantly higher than that for the HC group (12.7 ± 1.38 mM). Severe disruption of the rumen epithelium induced by HC was also ameliorated by dietary SB. Therefore, local inflammation and disruption of the rumen epithelium induced by HC were alleviated with SB administration.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Dai
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Xinxin
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Jinyu
authors.initials: J
authors.lastname: Yan
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Zain Ul
authors.initials: ZU
authors.lastname: Aabdin
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Muhammad Shahid
authors.initials: MS
authors.lastname: Bilal
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University , Nanjing 210095, P. R. China.
authors.forename: Xiangzhen
authors.identifier: http://orcid.org/0000-0002-3020-5010
authors.initials: X
authors.lastname: Shen
mesh_terms: D000821:Animal Feed / Q000009:adverse effects*; D000818:Animals; D020148:Butyric Acid / Q000008:administration & dosage*; D016207:Cytokines / Q000235:genetics / Q000276:immunology; D004032:Diet / Q000009:adverse effects*; D004848:Epithelium / Q000187:drug effects / Q000276:immunology*; D005260:Female; D015511:Goat Diseases / Q000188:drug therapy* / Q000276:immunology / Q000378:metabolism; D006041:Goats / Q000254:growth & development / Q000276:immunology / Q000378:metabolism; D007774:Lactation; D012417:Rumen / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism
chemical_list: D016207:Cytokines; D020148:Butyric Acid
keywords: dairy goat; high-concentrate diet; inflammation; rumen epithelium; sodium butyrate