title: Longitudinal Analysis of Serum Autoantibody-Reactivities in Patients with Primary Open Angle Glaucoma and Optic Disc Hemorrhage.
abstract: BACKGROUND
  The aim of our current investigation was to analyze the autoantibody-reactivities of primary open angle glaucoma patients with optic disc hemorrhage as possibly correlated to disease progression by means of a protein microarray approach.
  
  
  METHODS
  Sera of patients with primary open angle glaucoma and optic disc hemorrhage (n = 16) were collected directly after study inclusion (0 weeks) and after 2 weeks, 4 weeks and 12 weeks. As a control group patients with primary open angle glaucoma (n = 18) were used (0 weeks and 12 weeks). Microarrays were incubated and occurring antibody-antigen-reactions were visualized with fluorescence labeled anti-human-IgG secondary antibodies. To detect changes in autoantibodies spot intensities were digitized and compared.
  
  
  RESULTS
  With respect to the immunoreactivity at 0 weeks level increment of anti-adaptor protein 1 complex subunit mu-1 antibodies and anti-SPRY domain-containing SOCS box protein 3 antibodies in sera of primary open angle patients with optic disc hemorrhage was detected. Linear trend analysis revealed a positive correlation with r ≥ 0.8 between antibody-level and time course. Control group show no relevant changes in the same period. Significant changes were found in time point 4 comparison between patient groups in anti-adaptor protein 1 complex subunit mu-1-level (p = 0.01). No significant changes in visual acuity were found.
  
  
  CONCLUSIONS
  With this approach we were able to detect autoimmune reactivities in sera of patients with primary open angle glaucoma and optic disc hemorrhage compared to patients without optic disc hemorrhage. These antibodies could give further insights into the pathogenesis and the autoimmune component of glaucomatous optic neuropathy.
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Katrin
authors.initials: K
authors.lastname: Lorenz
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.|Department of Pharmacy and Biochemistry, Johannes Gutenberg-University Mainz, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Beck
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.|Vision 100 Die Augenärzte, Gemeinschaftspraxis Mönchengladbach, Germany.
authors.forename: Munir M
authors.initials: MM
authors.lastname: Keilani
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Joanna
authors.initials: J
authors.lastname: Wasielica-Poslednik
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Pfeiffer
authors.affiliation: Department of Experimental Ophthalmology, University Medical Center, Johannes Gutenberg-University, Mainz, Germany.
authors.forename: Franz H
authors.initials: FH
authors.lastname: Grus
mesh_terms: D000368:Aged; D001323:Autoantibodies / Q000097:blood*; D018450:Disease Progression; D005260:Female; D005902:Glaucoma, Open-Angle / Q000097:blood / Q000150:complications* / Q000276:immunology / Q000378:metabolism; D006801:Humans; D008137:Longitudinal Studies; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis
chemical_list: D001323:Autoantibodies