title: Amylopectin small chain glucans form structure fingerprint that determines botanical origin of starch.
abstract: Starch granule size, shape and structure of amylopectin are species specific and influence starch properties and end-use of starch. Amylopectin glucan chain structure was used to predict the starch botanical sources. Mathematical probability for accumulation of small glucan chains DP 6-10 reveal exponential fit curve with maximum R2in smallest granule size starches (Chlamydomonas, quinoa, buckwheat). Cereal and cassava showed R2of 0.81-0.96 while in pulses and tubers it was less than 0.7. The amylopectin small glucan chains form a unique 'finger print region' that identified starch botanical source. Differential amylopectin chain length distribution (APCLD) graphs between DP 6-80 of all species from Chlamydomonas starch distinguished five structural groups that clustered the 31 analyzed starches into four major patterns. APCLD analyses of amylopectin combined with characteristic pattern of small linear DP (6-9) glucan chains predicted the starch botanical source.
authors.affiliation: Department of Plant Sciences and Crop Developmental Centre, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N5A8, Canada. Electronic address: saj105@mail.usask.ca.
authors.forename: Sarita
authors.initials: S
authors.lastname: Jaiswal
authors.affiliation: Department of Plant Sciences and Crop Developmental Centre, College of Agriculture and Bioresources, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N5A8, Canada. Electronic address: ravi.chibbar@usask.ca.
authors.forename: Ravindra N
authors.initials: RN
authors.lastname: Chibbar
mesh_terms: D000687:Amylopectin / Q000737:chemistry*; D000688:Amylose; D027465:Chenopodium quinoa; D002696:Chlamydomonas; D002523:Edible Grain; D005936:Glucans; D002365:Manihot; D035281:Plant Tubers; D013213:Starch / Q000145:classification*
chemical_list: D005936:Glucans; D013213:Starch; D000688:Amylose; D000687:Amylopectin
keywords: Amylopectin chain length distribution; Amylopectin chain subtraction graph; Beans; Botanical starches; Cereals; Pulses; Small glucan chain fingerprint region; Tubers