title: Implementation of a Rapid Genotypic Assay to Promote Targeted Ciprofloxacin Therapy of Neisseria gonorrhoeae in a Large Health System.
abstract: Multidrug-resistant Neisseria gonorrhoeae is a top threat to public health. In November 2015, UCLA Health introduced a rapid gyrase A (gyrA) genotypic assay for prediction of Neisseria gonorrhoeae susceptibility to ciprofloxacin. We found a significant reduction in ceftriaxone use with a concomitant increase in targeted therapy.
authors.affiliation: David Geffen School of Medicine, University of California Los Angeles, CA, USA.
authors.forename: Lao-Tzu
authors.initials: LT
authors.lastname: Allan-Blitz
authors.affiliation: Department of Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.
authors.forename: Romney M
authors.initials: RM
authors.lastname: Humphries
authors.affiliation: Department of Laboratory Medicine, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.
authors.forename: Peera
authors.initials: P
authors.lastname: Hemarajata
authors.affiliation: Fielding School of Public Health, University of California, Los Angeles (UCLA), USA.
authors.forename: Ashima
authors.initials: A
authors.lastname: Bhatti
authors.affiliation: Alameda County Department of Public Health, Oakland, California, USA.
authors.forename: Mark W
authors.initials: MW
authors.lastname: Pandori
authors.affiliation: Department of Medicine, Harvard Medical School and Massachusetts General Hospital, Boston, USA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Siedner
authors.affiliation: Division of Infectious Diseases, Department of Medicine, David Geffen School of Medicine, UCLA, Los Angeles, California, USA.|Department of Epidemiology, Fielding School of Public Health, UCLA, California, USA.
authors.forename: Jeffrey D
authors.initials: JD
authors.lastname: Klausner
mesh_terms: D000900:Anti-Bacterial Agents / Q000627:therapeutic use*; D002443:Ceftriaxone / Q000627:therapeutic use; D002939:Ciprofloxacin / Q000627:therapeutic use*; D027081:DNA Gyrase / Q000235:genetics; D004363:Drug Utilization; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D006069:Gonorrhea / Q000188:drug therapy* / Q000382:microbiology*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests / Q000379:methods*; D009344:Neisseria gonorrhoeae / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D002443:Ceftriaxone; D027081:DNA Gyrase
keywords: Neisseria gonorrhoeae; antibiotic resistance.; ciprofloxacin; gyrA