title: Triterpenes suppress octanoylated ghrelin production in ghrelin-expressing human gastric carcinoma cells.
abstract: Ghrelin is an appetite-stimulating peptide hormone with an octanoyl modification at serine 3 that is essential for its orexigenic effect. Ghrelin O-acyltransferase (GOAT) is the enzyme that catalyzes ghrelin acylation using fatty acyl-coenzyme A as a substrate. We previously developed an assay system based on the AGS-GHRL8 cell line that produces octanoylated ghrelin in the presence of octanoic acid, and demonstrated that some fatty acids suppressed octanoylated ghrelin production. Recent studies have reported that triterpenes have anti-obesity effect. Since such triterpenes, like fatty acids, have a carboxyl group, we speculated that they can suppress octanoylated ghrelin production. To test this hypothesis, we investigated the effect of triterpenes on octanoylated ghrelin production. Asiatic acid, corosolic acid, glycyrrhetinic acid, oleanolic acid and ursolic acid suppressed octanoylated ghrelin levels in AGS-GHRL8 cells without decreasing transcript expression of GOAT or furin, a protease required for ghrelin maturation. β-amyrin had no effect on octanoylated ghrelin level, which was only slightly inhibited by uvaol; the fact that both these triterpenes lack a carboxyl group indicates that this group is important for suppressing octanoylated ghrelin production. These results suggest that triterpenes may have the potential as obesity-preventing agents with suppressive effect on octanoylated ghrelin production.
authors.affiliation: Graduate School of Pharmaceutical Sciences, Nagasaki University.
authors.forename: Kensuke
authors.initials: K
authors.lastname: Nakajima
authors.forename: Shigeru
authors.initials: S
authors.lastname: Oiso
authors.forename: Takuhiro
authors.initials: T
authors.lastname: Uto
authors.forename: Osamu
authors.initials: O
authors.lastname: Morinaga
authors.forename: Yukihiro
authors.initials: Y
authors.lastname: Shoyama
authors.forename: Hiroko
authors.initials: H
authors.lastname: Kariyazono
mesh_terms: D000217:Acyltransferases / Q000235:genetics / Q000378:metabolism; D000230:Adenocarcinoma / Q000235:genetics* / Q000378:metabolism*; D002210:Caprylates; D045744:Cell Line, Tumor; D045683:Furin / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D054439:Ghrelin / Q000235:genetics* / Q000378:metabolism*; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000378:metabolism*; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D002210:Caprylates; D054439:Ghrelin; D012333:RNA, Messenger; D014315:Triterpenes; D000217:Acyltransferases; C542776:MBOAT4 protein, human; C468731:FURIN protein, human; D045683:Furin; C031492:octanoic acid