title: Investigation of diverse bacteria in cloud water at Mt. Tai, China.
abstract: Bacteria are abundant in atmospheric water phase with the potential to influence atmospheric processes and human health, yet relatively little information is known about the bacterial characteristics at high altitudes. Here we investigated the bacterial community by high throughput sequencing in 24 cloud water samples collected from September 26 to October 31, at the summit of Mt. Tai (36°15' N, 117°06' E, 1534m a.s.l) in China. Diverse bacterial population were identified and the gram-negative bacteria contributed the majority of total bacteria including Proteobacteria (81.6%) and Bacteroidetes (3.9%), followed by gram-positive bacteria Firmicutes (7.1%) and Actinobacteria (2.3%). These gram-negative taxa mainly inhabited in leaf-surface and cold environments. Meanwhile bacteria involved in the cloud condensation nuclei and ice nuclei formation were observed such as Sphingomonas (6.7%), Pseudomonas (4.1%), and Bacillus (1.1%). In addition, Sphingmonas was more active than that in daytime and participated in the cloud chemistry process. Meanwhile O3and SO2critically contributed to the variation of bacterial community. It is the first report on the bacterial community structure of cloud water over Asian area. Our results can serve as an important reference for environmental scientists, and biologists.
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Caihong
authors.initials: C
authors.lastname: Xu
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Min
authors.initials: M
authors.lastname: Wei
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China; Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China. Electronic address: jmchen@sdu.edu.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Chen
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Sui
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhu
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Jiarong
authors.initials: J
authors.lastname: Li
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Sui
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Li
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Wang
authors.affiliation: Environment Research Institute, School of Environmental Science and Engineering, Shandong University, Ji'nan 250100, China.
authors.forename: Qingzhu
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention (LAP), Fudan Tyndall Centre, Department of Environmental Science & Engineering, Fudan University, Shanghai 200433, China; Institut de Combustion, Aérothermique, Réactivité et Environnement, CNRS, 45071 Orléans cedex 02, France.
authors.forename: Abdelwahid
authors.initials: A
authors.lastname: Mellouki
mesh_terms: D000391:Air Microbiology; D001272:Atmosphere; D001419:Bacteria / Q000145:classification / Q000302:isolation & purification*; D002681:China; D004269:DNA, Bacterial; D004784:Environmental Monitoring; D005618:Fresh Water / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S; D014887:Weather
chemical_list: D004269:DNA, Bacterial; D012336:RNA, Ribosomal, 16S
keywords: 16S rRNA sequencing; Bacterial community; Bioaerosol; Cloud water