title: Co-immobilized Phosphorylated Cofactors and Enzymes as Self-Sufficient Heterogeneous Biocatalysts for Chemical Processes.
abstract: Enzyme cofactors play a major role in biocatalysis, as many enzymes require them to catalyze highly valuable reactions in organic synthesis. However, the cofactor recycling is often a hurdle to implement enzymes at the industrial level. The fabrication of heterogeneous biocatalysts co-immobilizing phosphorylated cofactors (PLP, FAD+, and NAD+) and enzymes onto the same solid material is reported to perform chemical reactions without exogeneous addition of cofactors in aqueous media. In these self-sufficient heterogeneous biocatalysts, the immobilized enzymes are catalytically active and the immobilized cofactors catalytically available and retained into the solid phase for several reaction cycles. Finally, we have applied a NAD+-dependent heterogeneous biocatalyst to continuous flow asymmetric reduction of prochiral ketones, thus demonstrating the robustness of this approach for large scale biotransformations.
authors.affiliation: Heterogeneous biocatalysis group, CIC biomaGUNE, Edificio Empresarial "C", Paseo de Miramón 182, 20009, Donostia, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Velasco-Lozano
authors.affiliation: Heterogeneous biocatalysis group, CIC biomaGUNE, Edificio Empresarial "C", Paseo de Miramón 182, 20009, Donostia, Spain.
authors.forename: Ana I
authors.initials: AI
authors.lastname: Benítez-Mateos
authors.affiliation: Heterogeneous biocatalysis group, CIC biomaGUNE, Edificio Empresarial "C", Paseo de Miramón 182, 20009, Donostia, Spain.|IKERBASQUE, Basque Foundation for Science, Bilbao, Spain.
authors.forename: Fernando
authors.identifier: 0000-0003-0031-1880
authors.initials: F
authors.lastname: López-Gallego
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D001711:Biotransformation; D003067:Coenzymes / Q000737:chemistry / Q000378:metabolism*; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism*; D005560:Formate Dehydrogenases / Q000737:chemistry / Q000378:metabolism*; D010766:Phosphorylation
chemical_list: D003067:Coenzymes; D004800:Enzymes, Immobilized; D000426:Alcohol Dehydrogenase; D005560:Formate Dehydrogenases
keywords: biocatalysis; cofactor regeneration; heterogenous catalysis; porous materials; protein immobilization