title: Decreased eicosapentaenoic acid levels in acne vulgaris reveals the presence of a proinflammatory state.
abstract: This study aimed to determine circulating levels of polyunsaturated fatty acids (PUFAs), secretory phospholipase A2 (sPLA2), lipoprotein lipase (LPL) and measure circulating protein levels of angiopoietin-like protein 3 (ANGPTL3), ANGPTL4, cyclooxygenase-2 (COX-2) and prostaglandin E2 (PGE2) in patients with acne vulgaris. Serum from 21 control subjects and 31 acne vulgaris patients were evaluated for levels of arachidonic acid (AA, C20:4n- 6), dihomo-gamma-linolenic acid (DGLA, C20:3n-6), eicosapentaenoic acid (EPA, C20:5n-3) and docosahexaenoic acid (DHA, C22:6n-3). PUFA levels were determined by an optimized multiple reaction monitoring (MRM) method using ultra fast-liquid chromatography (UFLC) coupled with tandem mass spectrometry (MS/MS). Lipid profile, routine biochemical and hormone parameters were assayed by standard kit methods Serum EPA levels were significantly decreased while AA/EPA and DGLA/EPA ratio were significantly increased in acne vulgaris patients compared to controls. Serum levels of AA, DGLA and DHA showed no significant difference while activity of sPLA2 and LPL were significantly increased in acne vulgaris compared to controls. Results of this study reveal the presence of a proinflammatory state in acne vulgaris as shown by significantly decreased serum EPA levels and increased activity of sPLA2, AA/EPA and DGLA/EPA ratio. Increased LPL activity in the serum of acne vulgaris patients can be protective through its anti-dyslipidemic actions. This is the first study reporting altered EPA levels and increased sPLA2 activity in acne vulgaris and supports the use of omega-3 fatty acids as adjuvant treatment for acne patients.
authors.affiliation: Endocrinology Clinic, Antalya Training and Research Hospital, University of Health Sciences, Antalya, Turkey.
authors.forename: İbrahim
authors.initials: İ
authors.lastname: Aslan
authors.affiliation: Department of Medical Biochemistry, Akdeniz University Medical Faculty, Antalya, Turkey.
authors.forename: Filiz
authors.initials: F
authors.lastname: Özcan
authors.affiliation: Dermatology Clinic, Antalya Training and Research Hospital, University of Health Sciences, Antalya, Turkey.
authors.forename: Taner
authors.initials: T
authors.lastname: Karaarslan
authors.affiliation: Department of Medical Biochemistry, Akdeniz University Medical Faculty, Antalya, Turkey.
authors.forename: Ebru
authors.initials: E
authors.lastname: Kıraç
authors.affiliation: Department of Medical Biochemistry, Akdeniz University Medical Faculty, Antalya, Turkey. Electronic address: mutayaslan@akdeniz.edu.tr.
authors.forename: Mutay
authors.initials: M
authors.lastname: Aslan
mesh_terms: D000152:Acne Vulgaris / Q000097:blood* / Q000201:enzymology; D000293:Adolescent; D000328:Adult; D000091303:Angiopoietin-Like Protein 3; D000075803:Angiopoietin-Like Protein 4 / Q000097:blood; D000075802:Angiopoietin-like Proteins / Q000097:blood; D002648:Child; D051546:Cyclooxygenase 2 / Q000097:blood; D015232:Dinoprostone / Q000097:blood; D015118:Eicosapentaenoic Acid / Q000097:blood*; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood; D008071:Lipoprotein Lipase / Q000097:blood; D008297:Male; D055815:Young Adult
chemical_list: C479896:ANGPTL3 protein, human; D000091303:Angiopoietin-Like Protein 3; D000075803:Angiopoietin-Like Protein 4; D000075802:Angiopoietin-like Proteins; D015118:Eicosapentaenoic Acid; D051546:Cyclooxygenase 2; D008071:Lipoprotein Lipase; D015232:Dinoprostone
keywords: Acne vulgaris; Lipoprotein lipase; Polyunsaturated fatty acid; Secretory phospholipase A2