title: Resistance of green lacewing, Chrysoperla carnea Stephens to nitenpyram: Cross-resistance patterns, mechanism, stability, and realized heritability.
abstract: The green lacewing, Chrysoperla carnea Stephens (Neuroptera: Chrysopidae) is a major generalist predator employed in integrated pest management (IPM) plans for pest control on many crops. Nitenpyram, a neonicotinoid insecticide has widely been used against the sucking pests of cotton in Pakistan. Therefore, a field green lacewing strain was exposed to nitenpyram for five generations to investigate resistance evolution, cross-resistance pattern, stability, realized heritability, and mechanisms of resistance. Before starting the selection with nitenpyram, a field collected strain showed 22.08-, 23.09-, 484.69- and 602.90-fold resistance to nitenpyram, buprofezin, spinosad and acetamiprid, respectively compared with the Susceptible strain. After continuous selection for five generations (G1-G5) with nitenpyram in the laboratory, the Field strain (Niten-SEL) developed a resistance ratio of 423.95 at G6. The Niten-SEL strain at G6 showed no cross-resistance to buprofezin and acetamiprid and negative cross-resistance to spinosad compared with the Field strain (G1). For resistance stability, the Niten-SEL strain was left unexposed to any insecticide for four generations (G6-G9) and bioassay results at G10 showed that resistance to nitenpyram, buprofezin and spinosad was stable, while resistance to acetamiprid was unstable. The realized heritability values were 0.97, 0.16, 0.03, and -0.16 to nitenpyram, buprofezin, acetamiprid and spinosad, respectively, after five generations of selection. Moreover, the enzyme inhibitors (PBO or DEF) significantly decreased the nitenpyram resistance in the resistant strain, suggesting that resistance was due to microsomal oxidases and esterases. These results are very helpful for integration of green lacewings in IPM programs.
authors.affiliation: Department of Entomology, University College of Agriculture, University of Sargodha, Sargodha, Pakistan. Electronic address: honeybeepak@gmail.com.
authors.forename: Muhammad Mudassir
authors.initials: MM
authors.lastname: Mansoor
authors.affiliation: Department of Entomology, University College of Agriculture, University of Sargodha, Sargodha, Pakistan.
authors.forename: Abu Bakar Muhammad
authors.initials: AB
authors.lastname: Raza
authors.affiliation: Department of Entomology, University College of Agriculture and Environmental Sciences, Islamia University of Bahawalpur, Bahawalpur, Pakistan.
authors.forename: Naeem
authors.initials: N
authors.lastname: Abbas
authors.affiliation: Department of Entomology, University College of Agriculture, University of Sargodha, Sargodha, Pakistan.
authors.forename: Muhammad Anjum
authors.initials: MA
authors.lastname: Aqueel
authors.affiliation: Department of Entomology, University College of Agriculture, University of Sargodha, Sargodha, Pakistan.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Afzal
mesh_terms: D000818:Animals; D065607:Cytochrome P-450 Enzyme Inhibitors / Q000633:toxicity; D004950:Esterases / Q000037:antagonists & inhibitors; D040582:Inheritance Patterns; D007313:Insecta / Q000187:drug effects* / Q000235:genetics; D007305:Insecticide Resistance / Q000235:genetics; D007306:Insecticides / Q000633:toxicity*; D007928:Lethal Dose 50; D000073943:Neonicotinoids; D063086:Organothiophosphates / Q000633:toxicity; D010882:Piperonyl Butoxide / Q000633:toxicity; D011725:Pyridines / Q000633:toxicity*
chemical_list: D065607:Cytochrome P-450 Enzyme Inhibitors; D007306:Insecticides; D000073943:Neonicotinoids; D063086:Organothiophosphates; D011725:Pyridines; C464843:nitenpyram; C006863:butyl phosphorotrithioate; D004950:Esterases; D010882:Piperonyl Butoxide
keywords: Biochemical mechanism; Cross-resistance; Indigenous predator; Neonicotinoids; Resistance evolution