title: Design of antibacterial biointerfaces by surface modification of poly (ε-caprolactone) with fusion protein containing hydrophobin and PA-1.
abstract: Class IIa bacteriocin pediocin PA-1 has broad-spectrum activity and is a well-characterized candidate food biopreservative. Here, a simple approach is designed to extend the application of pediocin PA-1 in improving the antibacterial activity of electrospun poly(caprolactone) (PCL) grafts through combining PA-1 with HGFI, which is a self-assembled protein with characteristics allowing the modulation of surface properties of other materials originated from Grifola frondosa. Saccharomyces cerevisiae was used as the host for expression of fusion protein PA-1-linker-HGFI (pH) and his-tag purification was used to purify recombinant protein pH. An antibacterial activity assay showed the fusion protein pH retained the biological property of native PA-1. Water contact angle, X-ray photoelectron spectroscopy, immunofluorescence assay and atomic force microscopy indicated the surface properties of HGFI were greatly preserved by the fusion protein pH. Finally, antibacterial activity of pH-modified PCL substrate measurements implied the fusion protein significantly improved the bacterial-resistance of the PCL film through dressing the PCL fibers with the recombinant pH protein. This work presents a new perspective on the application of hydrophobin and pediocin PA-1 in antibacterial medical devices.
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
authors.forename: Xiangxiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, No. 94 Weijin Road, Tianjin 300071, PR China.
authors.forename: Jiwei
authors.initials: J
authors.lastname: Mao
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, No. 94 Weijin Road, Tianjin 300071, PR China.
authors.forename: Yiming
authors.initials: Y
authors.lastname: Chen
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
authors.forename: Dongmin
authors.initials: D
authors.lastname: Song
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Gao
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
authors.forename: Xiuming
authors.initials: X
authors.lastname: Zhang
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Bai
authors.affiliation: Department of Food and Environmental Sciences, University of Helsinki, Finland.
authors.forename: Per E J
authors.initials: PEJ
authors.lastname: Saris
authors.affiliation: Environmental Protection Technical Development Center, Tianjin, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Feng
authors.affiliation: The Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, No. 94 Weijin Road, Tianjin 300071, PR China. Electronic address: xuhaijin@aliyun.com.
authors.forename: Haijin
authors.initials: H
authors.lastname: Xu
authors.affiliation: The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, PR China. Electronic address: qiaomq@nankai.edu.cn.
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Qiao
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D000906:Antibodies / Q000737:chemistry; D001430:Bacteriocins / Q000737:chemistry; D018441:Biofilms; D002208:Caproates / Q000737:chemistry*; D004864:Equipment and Supplies; D005520:Food Preservatives; D048691:Grifola / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D007783:Lactones / Q000737:chemistry*; D051379:Mice; D018625:Microscopy, Atomic Force; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D000070017:Pediocins / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D011993:Recombinant Fusion Proteins / Q000737:chemistry; D012441:Saccharomyces cerevisiae; D013499:Surface Properties; D017155:Wettability
chemical_list: D000900:Anti-Bacterial Agents; D000906:Antibodies; D001430:Bacteriocins; D002208:Caproates; D005520:Food Preservatives; D007783:Lactones; D000070017:Pediocins; D011993:Recombinant Fusion Proteins; C074288:pediocin PA-1; C121056:caprolactone
keywords: Antibacterial; Hydrophobin; Medical device; Pediocin PA-1; Poly(ε-caprolactone)