title: Enhanced adhesion and proliferation of human umbilical vein endothelial cells on conductive PANI-PCL fiber scaffold by electrical stimulation.
abstract: Recently, electrically conductive biomaterial scaffolds have shown great potential in tissue regeneration. Herein, we reported an electrically conductive polyaniline (PANI) coated poly(ε-caprolactone) (PCL) electrospun micron-fiber scaffold for the enhanced attachment and proliferation of human umbilical vein endothelial cells (HUVECs) under electrical stimulation conditions. After the O2plasma treatment toward PCL electrospun fiber, PANI could be polymerized onto their surfaces successfully. The obtained PANI-PCL fibers were characterized by SEM observations, FT-IR spectra, XPS analysis, and water contact angle measurement. The mechanical tests indicated that the fibers could satisfy the practical vascular scaffold requirements. The conductivity of the PANI-PCL fibers was 6.71×10-3S/cm which could provide a conductive in-vitro platform to study the effect of electrical stimulation on HUVECs proliferation. When PANI-coated PCL fibers were compared with PCL fibers, HUVECs exhibited highly enhanced adhesion and viability, especially under electrical stimulation (ES) of 200, 300, and 400mV/cm. Proliferation of HUVECs on PANI-PCL fibers was strongly dependent on electrical stimulation intensity. The results showed new insights into conductive scaffolds for vascular tissue engineering.
authors.affiliation: Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China; Department of Clinical Pharmacy and Traditional Chinese Medicine Pharmacology, School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, People's Republic of China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Li
authors.affiliation: Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Rui
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Clinical Pharmacy and Traditional Chinese Medicine Pharmacology, School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, Changchun 130117, People's Republic of China.
authors.forename: Chuying
authors.initials: C
authors.lastname: Wang
authors.affiliation: Chemistry and Biology Science College, Changchun University of Technology, Changchun 130012, People's Republic of China. Electronic address: qfp2004@126.com.
authors.forename: Fangping
authors.initials: F
authors.lastname: Qiu
authors.affiliation: Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Bolun
authors.initials: B
authors.lastname: Sun
authors.affiliation: Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: He
authors.initials: H
authors.lastname: Ji
authors.affiliation: Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China.
authors.forename: Ju
authors.initials: J
authors.lastname: Qiu
authors.affiliation: Alan G. MacDiarmid Institute, Jilin University, Changchun 130012, People's Republic of China. Electronic address: cwang@jlu.edu.cn.
authors.forename: Ce
authors.initials: C
authors.lastname: Wang
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004558:Electric Stimulation; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008855:Microscopy, Electron, Scanning; D056951:Photoelectron Spectroscopy; D058626:Plasma Gases; D011091:Polyesters / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D023822:Tissue Engineering; D017155:Wettability
chemical_list: D000814:Aniline Compounds; D001672:Biocompatible Materials; D058626:Plasma Gases; D011091:Polyesters; C416807:polyaniline; C016240:polycaprolactone
keywords: Conductive; Electrical stimulation; Electrospinning; PANI-PCL; Vascular tissue engineering