title: Antifungal Effect of MalaysianAloe veraLeaf Extract on Selected Fungal Species of Pathogenic Otomycosis Species in In Vitro Culture Medium.
abstract: OBJECTIVE
  Aloe barbadensis millerorAloe verahas been used for therapeutic purposes since ancient times with antifungal activity known to be amongst its medicinal properties. We conducted a pilot study to determine the antifungal properties of MalaysianAloe veraleaf extract on otomycosis species includingAspergillus nigerandCandida albicans.
  
  
  METHODS
  This laboratory-controlled prospective study was conducted at the Universiti Sains Malaysia. Extracts of MalaysianAloe veraleaf was prepared in ethanol and solutions via the Soxhlet extraction method. Sabouraud dextrose agar cultured with the two fungal isolates were inoculated with the five different concentrations of each extract (50 g/mL, 25 g/mL, 12.5 g/mL, 6.25 g/mL, and 3.125 g/mL) using the well-diffusion method. Zone of inhibition was measured followed by minimum inhibitory concentration (MIC).
  
  
  RESULTS
  ForA. niger,a zone of inhibition for alcohol and aqueous extract was seen for all concentrations except 3.125 g/mL. There was no zone of inhibition for both alcohol and aqueous extracts ofAloe veraleaf forC. albicans. The MIC values of aqueous and alcohol extracts were 5.1 g/mL and 4.4 g/mL forA. nigerand since no zone of inhibition was obtained forC. albicansthe MIC was not determined.
  
  
  CONCLUSIONS
  The antifungal effect of alcohol extracts of MalaysianAloe veraleaf is better than the aqueous extract forA. niger(p< 0.001). MalaysianAloe verahas a significant antifungal effect towardsA. niger.
authors.affiliation: Department of Otorhinolaryngology-Head & Neck Surgery, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kota Bharu, Kelantan, Malaysia.
authors.forename: Jeyasakthy
authors.initials: J
authors.lastname: Saniasiaya
authors.affiliation: Department of Otorhinolaryngology-Head & Neck Surgery, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kota Bharu, Kelantan, Malaysia.
authors.forename: Rosdan
authors.initials: R
authors.lastname: Salim
authors.affiliation: Department of Otorhinolaryngology-Head & Neck Surgery, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kota Bharu, Kelantan, Malaysia.
authors.forename: Irfan
authors.initials: I
authors.lastname: Mohamad
authors.affiliation: Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia Health Campus, 16150 Kota Bharu, Kelantan, Malaysia.
authors.forename: Azian
authors.initials: A
authors.lastname: Harun
keywords: Aloe vera; Aspergillus Niger; Candida Albicans; Otomycosis