title: Differential modulation of the chaperone-like activity of HSP-1/2, a major protein of horse seminal plasma by anionic and cationic surfactants.
abstract: The major protein of equine seminal plasma, HSP-1/2 exhibits chaperone-like activity (CLA) by protecting various target proteins against thermal, chemical and oxidative stress. Polydispersity and surface hydrophobicity of HSP-1/2 were found to be important for its CLA. Surfactants are known to alter certain properties of proteins, e.g. hydrophobicity, charge and conformation either by altering properties of the medium or by direct binding. In the current study, thermal aggregation of alcohol dehydrogenase (ADH) and enolase has been studied in the presence of HSP-1/2, different surfactants and their combinations. The results obtained show that anionic surfactants (SDS, sodium dodecyl benzene sulfate) and neutral surfactants (tween-20, triton X-100) increase the CLA of HSP-1/2 and also inhibit aggregation of the target proteins independently. On the other hand, cationic surfactants (CTAB, alanine palmityl ester) increased the thermal aggregation of ADH and enolase and also decreased the CLA of HSP-1/2. These results are of significant interest as they show that surfactants such as SDS and tween-20 can potentially be used as anti-aggregation agents to prevent thermal aggregation of target proteins.
authors.affiliation: School of Chemistry, University of Hyderabad, Hyderabad 500046, India.
authors.forename: C Sudheer
authors.initials: CS
authors.lastname: Kumar
authors.affiliation: School of Chemistry, University of Hyderabad, Hyderabad 500046, India. Electronic address: mjswamy@uohyd.ac.in.
authors.forename: Musti J
authors.initials: MJ
authors.lastname: Swamy
mesh_terms: D000426:Alcohol Dehydrogenase / Q000737:chemistry; D000818:Animals; D002352:Carrier Proteins / Q000737:chemistry / Q000378:metabolism*; D006023:Glycoproteins / Q000737:chemistry / Q000378:metabolism*; D006736:Horses; D057927:Hydrophobic and Hydrophilic Interactions; D010751:Phosphopyruvate Hydratase / Q000737:chemistry; D066329:Protein Aggregates; D017433:Protein Structure, Secondary / Q000187:drug effects; D012661:Semen / Q000737:chemistry*; D029589:Seminal Plasma Proteins / Q000737:chemistry / Q000378:metabolism*; D013501:Surface-Active Agents / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: D002352:Carrier Proteins; D006023:Glycoproteins; C095321:HSP-1 protein, Equus caballus; C106240:HSP-2 protein, Equus caballus; D066329:Protein Aggregates; D029589:Seminal Plasma Proteins; D013501:Surface-Active Agents; D000426:Alcohol Dehydrogenase; D010751:Phosphopyruvate Hydratase
keywords: Alcohol dehydrogenase; Anti-aggregation agent; Ionic surfactant