title: Characterization of new exopolysaccharide production by Rhizobium tropici during growth on hydrocarbon substrate.
abstract: Exopolysaccharide (EPS) are produced by a diverse of rhizobia species and has been demonstrated to be a bioemulsifier with potential applications in the degradation of hydrocarbons. In the present study, attempts were made to obtain the new exopolysaccharide production by Rhizobium tropici (SEMIA 4080 and MUTZC3) strains during growth on hydrocarbon substrate. Under the different cultivation conditions, the high molecular weight exopolysaccharides from Rhizobium tropici strains cultivated for 96h mainly consisted of carbohydrates (79-85%) and a low percentage of protein. The EPSC3-D differed from the others, with only 60% of carbohydrate. However, all strains produced polymers with distinct rheology properties, such as viscosity of each EPS sample, suitable for different applications. In addition, RP-HPLC, FTIR and NMR studies revealed EPS produced by rhizobia strains were similar indicating minimal difference between EPS compositions.
authors.affiliation: São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal. Electronic address: teluque@yahoo.com.br.
authors.forename: Tereza Cristina Luque
authors.initials: TCL
authors.lastname: Castellane
authors.affiliation: São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal.
authors.forename: João Carlos
authors.initials: JC
authors.lastname: Campanharo
authors.affiliation: Embrapa Instrumentação, Rua XV de Novembro 1452, São Carlos, São Paulo, 13560-970, Brazil.
authors.forename: Luiz Alberto
authors.initials: LA
authors.lastname: Colnago
authors.affiliation: Embrapa Instrumentação, Rua XV de Novembro 1452, São Carlos, São Paulo, 13560-970, Brazil.
authors.forename: Isabel Duarte
authors.initials: ID
authors.lastname: Coutinho
authors.affiliation: São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal.
authors.forename: Érica Mendes
authors.initials: ÉM
authors.lastname: Lopes
authors.affiliation: São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal.
authors.forename: Manoel Victor Franco
authors.initials: MVF
authors.lastname: Lemos
authors.affiliation: São Paulo State University (Unesp), School of Agricultural and Veterinarian Sciences, Jaboticabal. Electronic address: egerle@fcav.unesp.br.
authors.forename: Eliana Gertrudes
authors.initials: EG
authors.lastname: de Macedo Lemos
mesh_terms: D055598:Chemical Phenomena; D006838:Hydrocarbons / Q000378:metabolism*; D008970:Molecular Weight; D011135:Polysaccharides, Bacterial / Q000096:biosynthesis* / Q000737:chemistry*; D012212:Rheology; D042162:Rhizobium tropici / Q000254:growth & development* / Q000378:metabolism*; D014783:Viscosity
chemical_list: D006838:Hydrocarbons; D011135:Polysaccharides, Bacterial
keywords: Bioemulsifier; Exopolysaccharides; Rhizobium tropici