title: Differential expression of survival proteins during decreased intracellular oxygen tension in brain endothelial cells of grey mullets.
abstract: The brain requires constant oxygen supply to perform its biological functions essential for survival. Because of low oxygen capacity and poor oxygen diffusibility of water, many fish species have evolved various adaptive mechanisms to cope with depleted oxygen. Endothelial cells (EC) are the primary components responsible for controlled environment of brain. Brain homeostasis largely depends on integrity of the EC. To elucidate their adaptive strategy, EC were isolated from the fish brain of Kovalam-control site and Ennore estuary-test/field hypoxic site and were subjected to low oxygen tension in laboratory. Cell viability, 4-hydroxynonenal (4HNE) and total antioxidant capacity (TAC) were analyzed to ascertain stress. Hypoxic insult, cytoprotective role of HSPs and apoptotic effect were analyzed by assessing hypoxia-inducible-factor-α (HIF1α), heat-shock-protein-70 (HSP70), heme-oxygenase 1 (HO-1), and apoptosis signal regulating kinase-1 (ASK1). This study evidenced that HSP70 and HO-1 are the key stress proteins, confer high tolerance to decreased oxygen tension mediated stress.
authors.affiliation: P.G. Department of Biochemistry, Bharathi Women's College (A), Affiliated to University of Madras, Tamil Nadu, India. Electronic address: dstpadmini@rediffmail.com.
authors.forename: Padmini
authors.initials: P
authors.lastname: Ekambaram
authors.affiliation: P.G. Department of Biochemistry, Bharathi Women's College (A), Affiliated to University of Madras, Tamil Nadu, India.
authors.forename: Meenakshi
authors.initials: M
authors.lastname: Narayanan
authors.affiliation: P.G. Department of Biochemistry, Bharathi Women's College (A), Affiliated to University of Madras, Tamil Nadu, India.
authors.forename: Parimala
authors.initials: P
authors.lastname: Parasuraman
mesh_terms: D000818:Animals; D001921:Brain / Q000166:cytology; D042783:Endothelial Cells / Q000378:metabolism*; D029941:Fish Proteins / Q000378:metabolism*; D018384:Oxidative Stress; D010100:Oxygen / Q000378:metabolism*; D023701:Smegmamorpha / Q000378:metabolism*
chemical_list: D029941:Fish Proteins; D010100:Oxygen
keywords: ASK1; HO-1; HSP70; Low oxygen tension; Neurovascular endothelial cell