title: Structural characterization and immunomodulatory activity of a new polysaccharide from jellyfish.
abstract: A new polysaccharide (JSP-11) with a molecular weight of 1.25×106Da was extracted and purified from jellyfish. Monosaccharide analysis showed that JSP-11 was composed of mannose, galactose and glucuronic acid with a molar ratio of 2.18:1.00:1.94. According to the analysis of fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, JSP-11 was determined to contain a linear backbone which consisted of (1→3,6)-linked β-d-Manp and (1→6)-linked β-d-Galp. The branch of (1→)-linked α-d-GlcpA was attached to the C-3 position of (1→3,6)-linked β-d-Manp in the backbone. The immunomodulatory assay exhibited that JSP-11 could significantly enhance the viability of RAW 264.7 macrophage cells, and promote the release of NO, TNF-α, and IL-1β via activating NF-κB, MAPKs and PI3K/Akt signal pathways.
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, People's Republic of China.
authors.forename: Qiang-Ming
authors.initials: QM
authors.lastname: Li
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, People's Republic of China; Students' Affairs Division, Qilu University of Technology, Jinan 250300, Shandong Province, People's Republic of China.
authors.forename: Jing-Fei
authors.initials: JF
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, People's Republic of China; School of Biological and Medical Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, People's Republic of China. Electronic address: zhaxueqiang@hfut.edu.cn.
authors.forename: Xue-Qiang
authors.initials: XQ
authors.lastname: Zha
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, People's Republic of China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Pan
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, People's Republic of China.
authors.forename: Hai-Lin
authors.initials: HL
authors.lastname: Zhang
authors.affiliation: School of Food Science and Engineering, Hefei University of Technology, Hefei 230009, Anhui Province, People's Republic of China. Electronic address: jianpingluo@hfut.edu.cn.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Luo
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D005690:Galactose / Q000737:chemistry; D020723:Glucuronic Acid / Q000737:chemistry; D007155:Immunologic Factors / Q000737:chemistry / Q000494:pharmacology; D008358:Mannose / Q000737:chemistry; D051379:Mice; D009569:Nitric Oxide / Q000378:metabolism; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology*; D000067996:RAW 264.7 Cells; D037401:Scyphozoa / Q000737:chemistry*
chemical_list: D007155:Immunologic Factors; D011134:Polysaccharides; D009569:Nitric Oxide; D020723:Glucuronic Acid; D008358:Mannose; D005690:Galactose
keywords: Immunomodulatory activity; Jellyfish; Polysaccharide; Structural characterization