title: MicroRNA-567 dysregulation contributes to carcinogenesis of breast cancer, targeting tumor cell proliferation, and migration.
abstract: We demonstrated that Hsa-miR-567 expression is significantly downregulated in poor prognosis breast cancer, compared to better prognosis breast cancer, having a role in the control of cell proliferation and migration by regulating KPNA4 gene.
  
  
  
  In this study, based on our previously published in silico results, we proved both in vitro (cell line studies) and ex vivo (clinical studies), that Hsa-miR-567 expression is significantly downregulated in breast cancer with poor prognosis when compared to breast cancer with better prognosis. More intriguingly, we demonstrated that the ectopic expression of Hsa-miR-567 in poor prognosis breast cancer cell line strongly inhibits in vitro cell proliferation and migration. Furthermore, we showed in vivo that breast cancer cells, stably expressing Hsa-miR-567, xenografted in mouse, reduce tumor growth ability. Consistently, we found that karyopherin 4 (KPNA4), predicted target gene of Hsa-miR-567 as identified by our in silico analysis, is upregulated in highly aggressive MDA-MB-231 breast cancer cell line and patient tissues with poor prognosis with respect to good prognosis.
  
  
  
  Our results suggest a potential role of Hsa-miR-567 as a novel prognostic biomarker for BC and as regulator of KPNA4.
authors.affiliation: Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Via F.Cervi 93, 20090, Segrate-Milan, Milan, Italy. gloria.bertoli@ibfm.cnr.it.
authors.forename: Gloria
authors.identifier: 0000-0002-5901-4269
authors.initials: G
authors.lastname: Bertoli
authors.affiliation: Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Via F.Cervi 93, 20090, Segrate-Milan, Milan, Italy.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cava
authors.affiliation: Tecnomed Foundation, University of Milano-Bicocca, Monza, Italy.|Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy.
authors.forename: Cecilia
authors.initials: C
authors.lastname: Diceglie
authors.affiliation: Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy.
authors.forename: Cristina
authors.initials: C
authors.lastname: Martelli
authors.affiliation: Department of Medical Oncology, IRCCS Fondazione Maugeri, Pavia, Italy.
authors.forename: Giampiero
authors.initials: G
authors.lastname: Rizzo
authors.affiliation: Department of Medical Oncology, IRCCS Fondazione Maugeri, Pavia, Italy.|Laboratory of Experimental Oncology and Pharmacogenomics, Institutional Oncologic Bio-Bank "Bruno Boerci", IRCCS Fondazione Salvatore Maugeri, Pavia, Italy.
authors.forename: Francesca
authors.initials: F
authors.lastname: Piccotti
authors.affiliation: Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Via F.Cervi 93, 20090, Segrate-Milan, Milan, Italy.|Department of Pathophysiology and Transplantation, University of Milan, Milan, Italy.
authors.forename: Luisa
authors.initials: L
authors.lastname: Ottobrini
authors.affiliation: Institute of Molecular Bioimaging and Physiology, National Research Council (IBFM-CNR), Via F.Cervi 93, 20090, Segrate-Milan, Milan, Italy.
authors.forename: Isabella
authors.initials: I
authors.lastname: Castiglioni
mesh_terms: D000818:Animals; D001943:Breast Neoplasms / Q000235:genetics* / Q000473:pathology; D063646:Carcinogenesis / Q000235:genetics*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D004195:Disease Models, Animal; D015536:Down-Regulation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D020022:Genetic Predisposition to Disease; D064593:Heterografts; D006801:Humans; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D034622:RNA Interference; D015203:Reproducibility of Results; D047368:Tumor Burden; D028901:alpha Karyopherins / Q000235:genetics
chemical_list: C106259:KPNA4 protein, human; D035683:MicroRNAs; D028901:alpha Karyopherins
keywords: Biomarker; Breast cancer; MicroRNA/miRNA; Prognosis; Proliferation