title: Higher production of C-phycocyanin by nitrogen-free (diazotrophic) cultivation of Nostoc sp. NK and simplified extraction by dark-cold shock.
abstract: Nostoc sp. NK (KCTC 12772BP) was isolated and cultivated in a BG11 medium and a nitrate-free BG11 medium (BG110). To enhance C-phycocyanin (C-PC) content in the cells, different fluorescent lamps (white, plant, and red) were used as light sources for complementary chromatic adaptation (CCA). The maximum biomass productivity was 0.42g/L/d and 0.32g/L/d under BG11 and BG110conditions, respectively. The maximum C-PC contents were 8.4% (w/w) under white lamps, 13.6% (w/w) under plant lamps, and 18% (w/w) under BG110and the red light condition. The maximum C-PC productivity was 57.4mg/L/d in BG110under the red lamp condition. These results indicate that a higher C-PC content could be obtained under a diazotrophic condition and a CCA reaction. The C-PC could be released naturally from cells without any extraction processes, when Nostoc sp. NK was cultivated in the BG110medium with CO2aeration and put in dark conditions at 5°C.
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Green Chemistry and Environmental Biotechnology, University of Science & Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
authors.forename: Na Kyeong
authors.initials: NK
authors.lastname: Lee
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Green Chemistry and Environmental Biotechnology, University of Science & Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Green Chemistry and Environmental Biotechnology, University of Science & Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea.
authors.forename: Hee-Sik
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Cell Factory Research Center, Korea Research Institute of Bioscience & Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea; Green Chemistry and Environmental Biotechnology, University of Science & Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, Republic of Korea. Electronic address: cyahn@kribb.re.kr.
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Ahn
mesh_terms: D000222:Adaptation, Physiological; D018533:Biomass; D005591:Chemical Fractionation / Q000379:methods*; D003470:Culture Media; D007218:Industrial Microbiology / Q000379:methods*; D008029:Lighting; D009584:Nitrogen / Q000378:metabolism; D009586:Nitrogen Fixation; D046937:Nostoc / Q000378:metabolism*; D010798:Phycocyanin / Q000096:biosynthesis* / Q000302:isolation & purification
chemical_list: D003470:Culture Media; D010798:Phycocyanin; D009584:Nitrogen
keywords: Complementary chromatic adaptation (CCA); Cyanobacteria; Nitrogen fixation; Nostoc sp.; Phycocyanin