title: Molecularly Imprinted Membrane Electrospray Ionization for Direct Sample Analyses.
abstract: Typically dealing with practical samples with very complex matrices, ambient ionization mass spectrometry suffers from low detection sensitivity. In this study, molecular imprinting technology was explored and integrated with the membrane electrospray ionization (MESI) method for direct sample analyses. By enriching targeted analytes on molecularly imprinted membranes (MIMs), improvement (by 10- to 50-fold) in the limit of quantitation could be achieved, compared to conventional nanoelectrospray ionization methods or other ambient ionization methods. MIMs were prepared by cross-linking a synthesized molecularly imprinted polymer layer onto a polyvinylidene difluoride (PVDF) membrane. The characteristics of MIM in recognizing target analytes were investigated and verified. Experiments showed that MIM-ESI could provide satisfactory performances for direct quantification of targeted analytes in complex samples using mass spectroscopy (MS), and the quantitative performance of this methodology was validated. With the capability of target enrichment, the uses of MIM-ESI MS in different application fields were also demonstrated, including food safety, quantification of drug concentrations in blood, pesticide residues in soil, and antibiotic residues in milk.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China , 102206.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China , 310003.
authors.forename: Tianyi
authors.initials: T
authors.lastname: Li
authors.affiliation: School of Life Science, Beijing Institute of Technology , Beijing, China , 100081.
authors.forename: Liusheng
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Chemistry, Capital Normal University , Beijing, China , 100048.
authors.forename: Yingfeng
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Chemistry, Beijing Institute of Technology , Beijing, China , 100081.
authors.forename: Xuebin
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China , 102206.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China , 310003.
authors.forename: Jianguo
authors.initials: J
authors.lastname: Xu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China , 102206.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China , 310003.
authors.forename: Jinxing
authors.initials: J
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention , Beijing, China , 102206.|Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, China , 310003.
authors.forename: Mei
authors.identifier: 0000-0002-3877-0209
authors.initials: M
authors.lastname: Zhang
authors.affiliation: School of Life Science, Beijing Institute of Technology , Beijing, China , 100081.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000032:analysis; D002976:Clenbuterol / Q000652:urine; D006801:Humans; D057230:Limit of Detection; D008892:Milk / Q000737:chemistry; D054802:Molecular Imprinting; D036103:Nanotechnology; D010573:Pesticide Residues / Q000032:analysis; D004364:Pharmaceutical Preparations / Q000097:blood; D011145:Polyvinyls / Q000737:chemistry; D012987:Soil / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D005465:Fluorocarbon Polymers
chemical_list: D000900:Anti-Bacterial Agents; D002976:Clenbuterol; D010573:Pesticide Residues; D004364:Pharmaceutical Preparations; D011145:Polyvinyls; D012987:Soil; C024865:polyvinylidene fluoride; D005465:Fluorocarbon Polymers