title: One-step isolation of carnosic acid and carnosol from rosemary by centrifugal partition chromatography.
abstract: Carnosic acid and carnosol are the main bioactive components responsible for the significant antioxidant activity of Rosmarinus officinalis. Nevertheless, they are known for their instability in solutions. Separation of both compounds from crude rosemary extract was successfully achieved by one-step centrifugal partition chromatography without any degradation. A two-phase solvent system, hexane/ethyl acetate/methanol/water (3:2:3:2 v/v) was run on a preparative scale applying the elution-extrusion technique in descending mode. A 900 mg quantity of the crude extract containing 39.7% carnosic acid and 12.3% carnosol was loaded onto a 500 mL column, rotating at 1800 rpm. Carnosic acid and carnosol were obtained at purities of 96.1 ± 1% and 94.4 ± 0.9%, with recoveries of 94.3 ± 4.4% and 94.8 ± 2.3%, respectively. The compounds were identified by mass spectrometry, tandem mass spectrometry, and comparison with authentic standards.
authors.affiliation: Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA.
authors.forename: Mary H
authors.initials: MH
authors.lastname: Grace
authors.affiliation: Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA.|School of Pharmacy, Lanzhou University, Lanzhou, P.R. China.
authors.forename: Yin
authors.initials: Y
authors.lastname: Qiang
authors.affiliation: Center for Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, North Carolina Research Campus, Kannapolis, NC, USA.
authors.forename: Shengmin
authors.initials: S
authors.lastname: Sang
authors.affiliation: Plants for Human Health Institute, Food Bioprocessing and Nutrition Sciences Department, North Carolina State University, North Carolina Research Campus, NC, USA.
authors.forename: Mary Ann
authors.initials: MA
authors.lastname: Lila
mesh_terms: D045784:Abietanes / Q000302:isolation & purification*; D002845:Chromatography; D010936:Plant Extracts / Q000737:chemistry; D027542:Rosmarinus / Q000737:chemistry*
chemical_list: D045784:Abietanes; D010936:Plant Extracts; C068623:carnosol; C018381:salvin
keywords: Rosmarinus officinalis; carnosic acid; carnosol; centrifugal partition chromatography