title: miR-383 inhibits ovarian cancer cell proliferation, invasion and aerobic glycolysis by targeting LDHA.
abstract: MicroRNAs (miRNAs) are differentially expressed in various cancers and act as oncogenes or tumor suppressors. MiR-383 has been characterized as a cancer suppressor in several cancers. However, the exact expression patterns of miR-383 and the precise molecular mechanisms underlying its role in ovarian cancer have not been investigated thoroughly. In this study, we found that the expression of miR-383 was significantly downregulated in ovarian cancer tissues and ovarian cancer cell lines. Ectopic expression of miR-383 remarkably suppressed the ovarian cancer cell proliferation by enhancing cell apoptosis and significantly inhibited the invasion of ovarian cancer cells, while low expression of miR-383 exhibited the opposite effect. Bioinformatics analysis suggested LDHA as a novel target of miR-383, and miR-383 suppressed the expression level of LDHA mRNA by direct binding to its 3'-untranslated region (3'UTR). Expression of miR-383 was negatively correlated with LDHA in ovarian cancer tissues. In addition, modulation of miR-383 expression could affect the aerobic glycolysis in the ovarian cancer cells. Furthermore, Silencing of LDHA counteracted the effects of miR-383 suppression, while its overexpression reversed tumor inhibitory effects of miR-383. In conclusion, our study demonstrated that miR-383 regulated LDHA expression in ovarian cancer cells, thereby stunting glycolysis, cell proliferation and invasion.
authors.forename: R L
authors.initials: RL
authors.lastname: Han
authors.forename: F P
authors.initials: FP
authors.lastname: Wang
authors.forename: P A
authors.initials: PA
authors.lastname: Zhang
authors.forename: X Y
authors.initials: XY
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Li
mesh_terms: D020413:3' Untranslated Regions; D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006019:Glycolysis; D006801:Humans; D007770:L-Lactate Dehydrogenase / Q000235:genetics / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics*; D010051:Ovarian Neoplasms / Q000235:genetics / Q000473:pathology*
chemical_list: D020413:3' Untranslated Regions; C558685:MIRN383 microRNA, human; D035683:MicroRNAs; D007770:L-Lactate Dehydrogenase
keywords: LDHA; miR-383 aerobic glycolysis.; ovarian cancer