title: SNEVhPrp19/hPso4Regulates Adipogenesis of Human Adipose Stromal Cells.
abstract: Aging is accompanied by loss of subcutaneous adipose tissue. This may be due to reduced differentiation capacity or deficiency in DNA damage repair (DDR) factors. Here we investigated the role of SNEVhPrp19/hPso4, which was implicated in DDR and senescence evasion, in adipogenic differentiation of human adipose stromal cells (hASCs). We showed that SNEV is induced during adipogenesis and localized both in the nucleus and in the cytoplasm. Knockdown of SNEV perturbed adipogenic differentiation and led to accumulation of DNA damage in hASCs upon oxidative stress. In addition, we demonstrated that SNEV is required for fat deposition in Caenorhabditis elegans. Consequently, we tested other DDR factors and found that WRN is also required for adipogenesis in both models. These results demonstrate that SNEV regulates adipogenesis in hASCs and indicate that DDR capacity in general might be a pre-requisite for this process.
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Department of Bioinformatics and Biotechnology of Faculty of Basic and Applied Sciences, International Islamic University, 44000 Islamabad, Pakistan.
authors.forename: Abdulhameed
authors.initials: A
authors.lastname: Khan
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Christian Doppler Laboratory on Biotechnology of Skin Aging, 1190 Vienna, Austria.
authors.forename: Hanna
authors.initials: H
authors.lastname: Dellago
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Christian Doppler Laboratory on Biotechnology of Skin Aging, 1190 Vienna, Austria.
authors.forename: Lucia
authors.initials: L
authors.lastname: Terlecki-Zaniewicz
authors.affiliation: Department of Phoniatrics, ENT University Hospital, Medical University of Graz, 8036 Graz, Austria.
authors.forename: Michael
authors.initials: M
authors.lastname: Karbiener
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Weilner
authors.affiliation: Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; Red Cross Blood Transfusion Service of Upper Austria, 4017 Linz, Austria.
authors.forename: Florian
authors.initials: F
authors.lastname: Hildner
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria.
authors.forename: Viktoria
authors.initials: V
authors.lastname: Steininger
authors.affiliation: Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; Red Cross Blood Transfusion Service of Upper Austria, 4017 Linz, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Gabriel
authors.affiliation: Research Institute for Biomedical Aging Research, Center for Molecular Biosciences (CMBI), University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Christoph
authors.initials: C
authors.lastname: Mück
authors.affiliation: Research Institute for Biomedical Aging Research, Center for Molecular Biosciences (CMBI), University of Innsbruck, 6020 Innsbruck, Austria.
authors.forename: Pidder
authors.initials: P
authors.lastname: Jansen-Dürr
authors.affiliation: Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria; Ludwig Boltzmann Institute for Experimental and Clinical Traumatology, AUVA Research Center, 1200 Vienna, Austria.
authors.forename: Ara
authors.initials: A
authors.lastname: Hacobian
authors.affiliation: Institute for Diabetes and Cancer (IDC), Helmholtz Zentrum München, German Research Center for Environmental Health, 85764 Neuherberg, Germany; Joint Heidelberg-IDC Translational Diabetes Program, Heidelberg University Hospital, 69120 Heidelberg, Germany; Molecular Metabolic Control, Medical Faculty, Technical University Munich, 80333 Munich, Germany; German Center for Diabetes Research (DZD), 85764 Neuherberg, Germany.
authors.forename: Marcel
authors.initials: M
authors.lastname: Scheideler
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria.
authors.forename: Regina
authors.initials: R
authors.lastname: Grillari-Voglauer
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria. Electronic address: markus.schosserer@boku.ac.at.
authors.forename: Markus
authors.initials: M
authors.lastname: Schosserer
authors.affiliation: Department of Biotechnology, BOKU -University of Natural Resources and Life Sciences, Vienna, 1190 Vienna, Austria; Christian Doppler Laboratory on Biotechnology of Skin Aging, 1190 Vienna, Austria; Austrian Cluster for Tissue Regeneration, 1200 Vienna, Austria. Electronic address: johannes.grillari@boku.ac.at.
authors.forename: Johannes
authors.initials: J
authors.lastname: Grillari
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D017173:Caenorhabditis elegans; D002454:Cell Differentiation / Q000235:genetics*; D004249:DNA Damage; D045643:DNA Repair Enzymes / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D055785:Gene Knockdown Techniques; D006801:Humans; D007328:Insulin / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics* / Q000378:metabolism; D018384:Oxidative Stress; D047495:PPAR gamma / Q000378:metabolism; D000072260:RNA Splicing Factors / Q000235:genetics* / Q000378:metabolism; D017154:Stromal Cells / Q000166:cytology* / Q000378:metabolism*
chemical_list: D007328:Insulin; D009687:Nuclear Proteins; D047495:PPAR gamma; D000072260:RNA Splicing Factors; D045643:DNA Repair Enzymes; C417041:PRPF19 protein, human
keywords: C. elegans; DNA damage repair; Prp19; Pso4; SNEV; WRN; adipogenesis; human adipose stromal cells