title: Angiogenesis in CD5-positive Diffuse Large B Cell Lymphoma: A Morphometric Analysis.
abstract: BACKGROUND
  CD5-positive diffuse large B cell lymphoma (DLBCL) is the least frequent immunohistochemical subgroup of DLBCL. The relatively little available data suggests a worse outcome in this population, resulting from a resistance to chemotherapy.
  
  
  OBJECTIVE
  The aim was the comparative assessment of angiogenesis in both CD5-positive and CD5-negative DLBCL, as well as in lymphatic tissues without lymphoproliferative diseases.
  
  
  METHODS
  The analysis included 36 cases of CD5-positive DLBCL (19 females and 17 males) aged 29-87 years (mean age 69), diagnosed and treated in the Maria Sklodowska-Curie Institute and Oncology Center and Medical University of Warsaw in 2002-2013. The control group comprised 28 cases of CD5-negative DLBCL (14 females and 14 males) aged 24-82 years (mean age 58.5). The secondary control group (13 cases) consisted of normal lymphatic tissue obtained from patients without lymphoproliferative diseases. The level of angiogenesis was assessed on the basis of immunohistochemical CD34, vWF and HIF1α expression measured using morphometric methods.
  
  
  RESULTS
  CD5-positive DLBCL, in comparison to CD5-negative DLBCL, was characterized by: (1) higher mean of total blood vessel area, (2) higher mean total ratio of blood vessel area and staining intensity, (3) higher mean of total blood vessel area in regions defined as hot spots, (4) higher mean of total ratio of blood vessel area and staining intensity in hot spots. The measurements in lymph nodes without lymphoproliferative diseases lay between the values obtained in both DLBCL subgroups.
  
  
  CONCLUSIONS
  We observed a significant exacerbation of angiogenesis in CD5-positive DLBCL in comparison to the CD5-negative subgroup, possibly explaining its more aggressive clinical course. Our data does not substantiate the hypothesis that angiogenesis is more pronounced in frequent CD5-negative DLBCL subgroup in comparison to benign lymphatic tissue.
authors.affiliation: Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie Institute and Oncology Center, Warszawa, Poland.
authors.forename: Nina
authors.initials: N
authors.lastname: Woźnialis
authors.affiliation: Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie Institute and Oncology Center, Warszawa, Poland.|Department of Pathology, Center for Biostructure Research, Medical University of Warsaw, Poland.
authors.forename: Beata
authors.initials: B
authors.lastname: Gierej
authors.affiliation: Department of Lymphoid Malignancies, Maria Sklodowska-Curie Institute and Oncology Center, Poland.
authors.forename: Lidia
authors.initials: L
authors.lastname: Popławska
authors.affiliation: Department of Hematology, Oncology and Internal Medicine, Medical University of Warsaw, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Ziarkiewicz
authors.affiliation: Department of Pathology, Center for Biostructure Research, Medical University of Warsaw, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Wolińska
authors.affiliation: Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie Institute and Oncology Center, Warszawa, Poland.
authors.forename: Elżbieta
authors.initials: E
authors.lastname: Kulczycka
authors.affiliation: Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie Institute and Oncology Center, Warszawa, Poland.|Department of Pathology, Center for Biostructure Research, Medical University of Warsaw, Poland.
authors.forename: Bogna
authors.initials: B
authors.lastname: Ziarkiewicz-Wróblewska
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000704:Analysis of Variance; D001808:Blood Vessels / Q000473:pathology; D018956:CD5 Antigens / Q000378:metabolism*; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D008198:Lymph Nodes / Q000473:pathology; D016403:Lymphoma, Large B-Cell, Diffuse / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009389:Neovascularization, Pathologic / Q000473:pathology*
chemical_list: D018956:CD5 Antigens
keywords: CD34; CD5-positive DLBCL; HIF1α; angiogenesis; vWF