title: Does Resveratrol Improve Insulin Signalling in HepG2 Cells?
abstract: OBJECTIVE
  Diabetes mellitus is a common metabolic disorder with high global prevalence. It is characterized by a decrease in insulin secretion or a decrease in insulin sensitivity or both. The aim of the present study was to investigate the effects of resveratrol treatment on the expression of the genes involved in insulin signalling cascade, such as Forkhead box protein O1 (FoxO1), 3-phosphoinositide-dependent protein kinase 1 (PDPK1) and mammalian target of rapamycin (mTOR).
  
  
  METHODS
  HepG2 cells were cultured in serum-free medium with high concentrations of glucose and insulin and then were treated with resveratrol (5, 10 and 20 µM) for 24 and 48 hours. Complementary deoxyribonucleic acids (cDNAs) were synthesized followed by RNA extraction. Real-time quantitative reverse transcription polymerase chain reaction was used to analyze the expression of FoxO1, PDPK1 and mTOR.
  
  
  RESULTS
  Resveratrol increased the expression of PDPK1, mTOR and FoxO1. No significant difference was seen among differing dosages of resveratrol, but treatments for 48 hours exerted the greatest effectiveness.
  
  
  CONCLUSIONS
  Our results were consistent with other studies showing the beneficial effects of resveratrol on diabetes. However, considering the effects of resveratrol in increasing FoxO1 and gluconeogenic gene expression, long-term usage of resveratrol should be investigated in greater depth in future studies.
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Marjan
authors.initials: M
authors.lastname: Norouzzadeh
authors.affiliation: Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Fatemehsadat
authors.initials: F
authors.lastname: Amiri
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ali Akbar
authors.initials: AA
authors.lastname: Saboor-Yaraghi
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Farnoosh
authors.initials: F
authors.lastname: Shemirani
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Yas
authors.initials: Y
authors.lastname: Kalikias
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Loghman
authors.initials: L
authors.lastname: Sharifi
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Monireh Sadat
authors.initials: MS
authors.lastname: Seyyedsalehi
authors.affiliation: Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetic, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: mahmoodi_maryam@yahoo.com.
authors.forename: Maryam
authors.initials: M
authors.lastname: Mahmoudi
mesh_terms: D064413:3-Phosphoinositide-Dependent Protein Kinases / Q000235:genetics / Q000378:metabolism; D000071161:Forkhead Box Protein O1 / Q000235:genetics / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D007328:Insulin / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D000077185:Resveratrol; D015398:Signal Transduction / Q000187:drug effects; D013267:Stilbenes / Q000494:pharmacology*; D058570:TOR Serine-Threonine Kinases / Q000235:genetics / Q000378:metabolism
chemical_list: C085126:FOXO1 protein, human; D000071161:Forkhead Box Protein O1; D007328:Insulin; D013267:Stilbenes; C546842:MTOR protein, human; D064413:3-Phosphoinositide-Dependent Protein Kinases; C578562:PDPK1 protein, human; D058570:TOR Serine-Threonine Kinases; D000077185:Resveratrol
keywords: FoxO1; HepG2; PDPK1; insulin resistance; insulinorésistance; mTOR; resveratrol; resvératrol