title: Virulence factors and antimicrobial susceptibility profile of extraintestinal Escherichia coli isolated from an avian colisepticemia outbreak.
abstract: In this study an avian colisepticemia outbreak was investigated. Two isolates from a chicken with colisepticemia were characterized for antimicrobial susceptibility and virulence factors profile. For this purpose 7 antimicrobial and 29 genes (fimH, hrlA/hek, iha, papC, sfa/focCD, tsh, mat, tia, gimB, ibeA, chuA, fyuA, ireA, iroN, irp2, iucD, sitD. chr., sitD. ep., iss, neuC, ompA, traT, astA, hlyA, sat, vat, pic, malX, cvi/cva) were tested. The outbreak happened in a hick chicken breeding located in the northwestern region of Rio Grande do Sul state in South of Brazil and caused 28.3% (102 deads of a total of 360 chickens) of mortality rate. Escherichia coli isolates obtained from the avian spleen and liver belong to the same phylogenetic group A and present resistance to all antimicrobials tested (ampicillin, tetracycline, gentamicin, neomycin, sulfa + trimethoprim, enrofloxacin, and norfloxacin). Both isolates harbor virulence factors related to adhesion (fimH, papC, mat), invasion (tia), iron acquisition system (iroN) and serum resistance (iss, ompA, traT), showing that these groups are important for Avian Pathogenic E. coli (APEC). However, they present different virulence profiles for some genes, whereas liver-isolate carries more hrlA/hek (adhesin), gimB (invasin), sitD ep. (iron acquisition system), sat (toxin) and hylA (toxin) genes, the spleen-isolate harbors fyuA (iron acquisition system) gene. Here, we highlight a coinfection by different strains of APEC in the same animal with colisepticemia, the great antimicrobial resistance of these bacterial isolates and the genetic traits that modulate the virulence for high mortality rate of chickens for human consumption.
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Jonas Fernandes
authors.initials: JF
authors.lastname: Maciel
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil; Departamento de Ciências da Saúde, Curso de Farmácia, Universidade Regional Integrada do Alto Uruguai e das Missões, campus Santo Ângelo, 98802-470, Rua Universidade das Missões, 464, Santo Ângelo, Rio Grande do Sul, Brazil. Electronic address: lmatter2006@yahoo.com.br.
authors.forename: Letícia Beatriz
authors.initials: LB
authors.lastname: Matter
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Michele Martins
authors.initials: MM
authors.lastname: Trindade
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Giovana
authors.initials: G
authors.lastname: Camillo
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Maristela
authors.initials: M
authors.lastname: Lovato
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil.
authors.forename: Sônia
authors.initials: S
authors.lastname: de Ávila Botton
authors.affiliation: Departamento de Medicina Veterinária Preventiva, Centro de Ciências Rurais, Universidade Federal de Santa Maria (UFSM), Prédio 44, Sala 5125, 97105-900, Av. Roraima, 1000, Santa Maria, Rio Grande do Sul, Brazil. Electronic address: agueda.vargas@gmail.com.
authors.forename: Agueda
authors.initials: A
authors.lastname: Castagna de Vargas
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002645:Chickens; D004196:Disease Outbreaks; D024881:Drug Resistance, Bacterial; D004926:Escherichia coli / Q000187:drug effects* / Q000472:pathogenicity; D004927:Escherichia coli Infections / Q000662:veterinary*; D005798:Genes, Bacterial; D008826:Microbial Sensitivity Tests; D011201:Poultry Diseases / Q000453:epidemiology* / Q000382:microbiology* / Q000401:mortality; D018805:Sepsis / Q000662:veterinary*; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D037521:Virulence Factors
keywords: Antimicrobial susceptibility; Avian colisepticemia; Escherichia coli; Virulence factors