title: New silver nanoparticles induce apoptosis-like process inE. coliand interfere with mammalian copper metabolism.
abstract: Silver nanoparticles (SNPs) are new functional materials that are widely used in biomedical and industrial technologies. Two main features that make SNPs valuable are their strong antibacterial effects and low toxicity to eukaryotes. In this study, SNPs were synthesized using a modified method of reducing the metal ions to their atomic state followed by crystallization. SNPs were characterized by UV/vis spectroscopy, X-ray diffractometry, atomic force microscopy, and transmission electron microscopy (TEM). The SNPs were spherically shaped with an average linear dimension of 20 nm. In aqueous solution, the SNPs were beige-yellow in color, and they formed a black color in bacteria-rich growth media. The toxicity and bioavailability of the SNPs were tested usingEscherichia colicells and C57Bl/6 mice. Although the SNPs displayed bactericidal activity, anE. colicell strain transformed with an expression plasmid carrying a human CTR1 ectodomain with three motives that bind Cu(II), Cu(I), and Ag(I) demonstrated increased resistance to treatment with SNPs. TEM showed that the SNPs were absorbed by theE. colicell, and flow cytometry showed that the SNPs induced apoptosis-like death. In mice treated with SNPs (daily intraperitoneal injection of 10 μg SNPs/g body weight over 4 days), the ceruloplasmin (Cp) oxidase activity in the blood serum decreased. However, level ofCpgene expression, the relative contents of the Cp protein in the Golgi complex and in the serum did not change. Treatment with SNPs did not influence the activity of superoxide dismutase 1 in the liver and had no apparent toxic effects in mice. These findings expand the scope of application for the use of new SNPs. The data are discussed in a paradigm, in which the effects of SNPs are caused by the interference of silver ions with copper metabolism.
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Department of Biophysics, Peter the Great St Petersburg Polytechnic University.
authors.forename: Iurii A
authors.initials: IA
authors.lastname: Orlov
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Department of Biophysics, Peter the Great St Petersburg Polytechnic University.
authors.forename: Tatiana P
authors.initials: TP
authors.lastname: Sankova
authors.affiliation: Department of Zoology, Herzen State Pedagogical University of Russia, St Petersburg.
authors.forename: Polina S
authors.initials: PS
authors.lastname: Babich
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Nanocatalysts and Functional Materials Laboratory, Togliatti State University, Togliatti.
authors.forename: Ilya M
authors.initials: IM
authors.lastname: Sosnin
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Department of Molecular Genetics, Research Institute of Experimental Medicine.
authors.forename: Ekaterina Yu
authors.initials: EY
authors.lastname: Ilyechova
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Ioffe Institute, St Petersburg, Russia.
authors.forename: Demid A
authors.initials: DA
authors.lastname: Kirilenko
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Ioffe Institute, St Petersburg, Russia.
authors.forename: Pavel N
authors.initials: PN
authors.lastname: Brunkov
authors.affiliation: Department of Zoology, Herzen State Pedagogical University of Russia, St Petersburg.
authors.forename: Gennadii L
authors.initials: GL
authors.lastname: Ataev
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Nanocatalysts and Functional Materials Laboratory, Togliatti State University, Togliatti; Ioffe Institute, St Petersburg, Russia.
authors.forename: Alexey E
authors.initials: AE
authors.lastname: Romanov
authors.affiliation: International Research and Education Center "Functional materials and devices of optoelectronics and microelectronics", ITMO University; Department of Biophysics, Peter the Great St Petersburg Polytechnic University; Department of Molecular Genetics, Research Institute of Experimental Medicine.
authors.forename: Ludmila V
authors.initials: LV
authors.lastname: Puchkova
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D015153:Blotting, Western; D002570:Ceruloplasmin / Q000235:genetics / Q000378:metabolism; D003300:Copper / Q000378:metabolism*; D003460:Crystallization; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism*; D005434:Flow Cytometry; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D008297:Male; D053768:Metal Nanoparticles / Q000008:administration & dosage* / Q000737:chemistry; D051379:Mice; D008810:Mice, Inbred C57BL; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D010084:Oxidation-Reduction; D012333:RNA, Messenger / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012834:Silver / Q000737:chemistry*; D000072105:Superoxide Dismutase-1 / Q000235:genetics / Q000378:metabolism
chemical_list: D000900:Anti-Bacterial Agents; D012333:RNA, Messenger; D012834:Silver; D003300:Copper; D000072105:Superoxide Dismutase-1; D002570:Ceruloplasmin
keywords: apoptosis-like death; bioavailability; copper status; mammalian copper metabolism; silver nanoparticles