title: Long non-coding RNA AK058003, as a precursor of miR-15a, interacts with HuR to inhibit the expression of γ-synuclein in hepatocellular carcinoma cells.
abstract: Long non-coding RNAs (lncRNAs) have been identified as critical players in multiple cancers and lncRNAs are tightly linked to cancer progression. However, only little amount of lncRNAs have been identified to participate in the molecular mechanisms of the progression of hepatocellular carcinoma. In this study, we found that lncRNA-AK058003 is down-regulated in hepatocellular carcinoma tissues and it is associated with the relapse and metastasis of the cancer. Furthermore, lncRNA-AK058003 acts as a tumor suppressor, suppressing hepatocellular carcinoma cell proliferation and metastasis in vitro and in vivo. lncRNA-AK058003 can reduce mRNA stabilizing protein HuR, which results in the inhibition of the expression of γ-synuclein. In addition, a bioinformatics study indicated that γ-synuclein is a target of miR-15a. To verify whether lncRNA-AK058003 plays a role in miR-15a-mediated inhibition of γ-synuclein, we demonstrated that lncRNA-AK058003 is very likely to be a precursor of miR-15a. Collectively, lncRNA-AK058003 can reduce the expression of mRNA stabilizing protein HuR and act as a precursor of miR-15a to suppress γ-synuclein-mediated cell proliferation and the metastasis of hepatocellular carcinoma.
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Xiaoqin
authors.initials: X
authors.lastname: He
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Yongfa
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Surgery, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Yuefeng
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Huilin
authors.initials: H
authors.lastname: Liang
authors.affiliation: Department of Biochemistry and Molecular Chemistry, School of Basic Medicial Sciences, Wuhan University, Wuhan, China.
authors.forename: Dongcheng
authors.initials: D
authors.lastname: Wu
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ge
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Junjian
authors.initials: J
authors.lastname: Deng
authors.affiliation: Cancer Center, Renmin Hospital of Wuhan University, Wuhan, China.
authors.forename: Ximing
authors.initials: X
authors.lastname: Xu
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000556:secondary; D002465:Cell Movement; D049109:Cell Proliferation; D019295:Computational Biology; D030541:Databases, Genetic; D015536:Down-Regulation; D000067780:ELAV-Like Protein 1 / Q000235:genetics / Q000378:metabolism*; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056945:Hep G2 Cells; D006801:Humans; D053208:Kaplan-Meier Estimate; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000401:mortality / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009363:Neoplasm Proteins / Q000235:genetics / Q000378:metabolism*; D016016:Proportional Hazards Models; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D051845:gamma-Synuclein / Q000235:genetics / Q000378:metabolism*
chemical_list: D000067780:ELAV-Like Protein 1; C098770:ELAVL1 protein, human; C505361:MIRN15 microRNA, human; D035683:MicroRNAs; D009363:Neoplasm Proteins; D062085:RNA, Long Noncoding; C104249:SNCG protein, human; D051845:gamma-Synuclein
keywords: liver cancer; lncRNA; mRNA stabilizing protein; tumor suppressor