title: Nocardioides phosphati sp. nov., an actinomycete isolated from a phosphate mine.
abstract: A Gram-staining-positive, non-spore-forming and non-motile strain, designated WYH11-7T, was isolated from a phosphate mine in Yunnan Province, PR China. The taxonomic position of WYH11-7T was investigated by polyphasic approaches. Phylogenetic analyses based on 16S rRNA gene sequences indicated that WYH11-7T represents a member of the genus Nocardioides. WYH11-7T was closely related to Nocardioidesjensenii DSM 20641T, Nocardioidesdubius DSM 19084T and Marmoricolaterrae DSM 27141T, and had pairwise 16S rRNA gene sequence similarities of 97.4, 97.2 and 97.0 %, respectively. DNA-DNA relatedness values between WYH11-7T and related type strains N. jensenii DSM 20641T and N. dubius DSM 19084T were found to be 17.6±4.9 and 14.6±3.1 %, respectively. The respiratory menaquinone of WYH11-7T was MK-8 (H4) while the major fatty acids were C18 : 1ω9c, C16 : 0, C17 : 0, C17 : 1ω8c, C18 : 1 10-methyl and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unidentified phospholipids. Whole-cell hydrolysates contained mannose, ribose, glucose and galactose along with ll-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The DNA G+C content was 71.2 mol%. Phenotypic, phylogenetic and chemotaxonomic data indicated that strain WYH11-7T represents a novel species of the genus Nocardioides, for which the name Nocardioidesphosphatisp. nov. is proposed. The type strain is WYH11-7T (=CGMCC 4.7371T=DSM 104026T).
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Fuhong
authors.initials: F
authors.lastname: Xie
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Huan
authors.initials: H
authors.lastname: Ma
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Shujing
authors.initials: S
authors.lastname: Quan
authors.affiliation: Henan Key Laboratory of Microbial Engineering, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Yue
authors.affiliation: Henan Key Laboratory of Microbial Engineering, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Yufei
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Henan Key Laboratory of Microbial Engineering, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Feng
authors.affiliation: Henan Key Laboratory of Microbial Engineering, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhen
authors.affiliation: Henan Engineering Research Center of Industrial Enzymes, Biology Institute of Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Dehai
authors.initials: D
authors.lastname: Liu
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D008906:Mining; D010457:Peptidoglycan / Q000737:chemistry; D010710:Phosphates; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010710:Phosphates; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C024223:vitamin MK 8; D003960:Diaminopimelic Acid