title: EF24 Suppresses Invasion and Migration of Hepatocellular Carcinoma CellsIn Vitrovia Inhibiting the Phosphorylation of Src.
abstract: Diphenyl difluoroketone (EF24), a curcumin analog, is a promising anticancer compound that exerts its effects by inhibiting cell proliferation and inducing apoptosis. However, the efficacy of EF24 against cancer metastasis, particularly in hepatocellular carcinoma (HCC), remains elusive. In this study, the effect of EF24 on HCCLM-3 and HepG2 cell migration and invasion was detected by wound healing and transwell assay, respectively. The results revealed that EF24 suppressed the migration and invasion of both HCCLM-3 and HepG2 cells. Furthermore, EF24 treatment decreased the formation of filopodia on the cell surface and inhibited the phosphorylation of Src in both cell lines, which may help contribute towards understanding the mechanism underlying the suppressive effect of EF24 on HCC migration and invasion. Additionally, the expression of total- and phosphorylated-Src in primary HCC tissues and their paired lymph node metastatic tissues was detected, and phosphorylated-Src was found to be associated with HCC lymph node metastasis. The results of this study suggest that Src is a novel and promising therapeutic target in HCC and provide evidence to support the hypothesis that EF24 may be a useful therapeutic agent for the treatment of HCC.
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Ran
authors.identifier: 0000-0003-3196-9554
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Department of Hepatopancreatobiliary Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong 266071, China.
authors.forename: Lamtin
authors.initials: L
authors.lastname: Tin
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Yuhua
authors.identifier: 0000-0001-9007-617X
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Yiqi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Yinji
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Xiaoming
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Microbiology, Harbin Medical University, Harbin, Heilongjiang 150081, China.
authors.forename: Fengmin
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Harbin Medical University, Harbin, Heilongjiang 150081, China; Translation Medicine Center of Northern China, Harbin Medical University, Harbin, Heilongjiang 150081, China; Basic Medical Institute, Heilongjiang Medical Science Academy, Heilongjiang 150081, China.
authors.forename: Xiaobo
authors.identifier: 0000-0003-1037-3903
authors.initials: X
authors.lastname: Li
mesh_terms: D001597:Benzylidene Compounds / Q000494:pharmacology*; D006528:Carcinoma, Hepatocellular / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D002465:Cell Movement / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D009361:Neoplasm Invasiveness; D010766:Phosphorylation / Q000187:drug effects; D010881:Piperidones / Q000494:pharmacology*; D016392:Proto-Oncogene Proteins pp60(c-src) / Q000378:metabolism*
chemical_list: C524042:3,5-bis(2-fluorobenzylidene)piperidin-4-one; D001597:Benzylidene Compounds; D010881:Piperidones; D016392:Proto-Oncogene Proteins pp60(c-src)