title: Antioxidant properties of mesenchymal stem cells against oxidative stress in a murine model of colitis.
abstract: OBJECTIVE
  To investigate the effects of oxidative stress injury in dextran sulfate sodium (DSS)-induced colitis in mice treated with mesenchymal stem cells (MSC).
  
  
  RESULTS
  Mice exposed to oral administration of 2% DSS over 7 days presented a high disease activity index and an intense colonic inflammation. Systemic infusion of MSC protected from severe colitis, reducing weight loss and diarrhea while lowering the infiltration of inflammatory cells. Moreover, toxic colitis injury increased oxidative stress. Administration of DSS decreased reduced glutathione (GSH) and superoxide dismutase (SOD) activity, and increased thiobarbituric acid-reactive substances levels in the colon. No alteration was found in catalase (CAT) and glutathione peroxidase (GPx) activity. Otherwise, MSC transplantation was able to prevent the decrease of GSH levels and SOD activity suggestive of an antioxidant property of MSC.
  
  
  CONCLUSIONS
  The oxidative stress is a pathomechanism underlying the pathophysiology of colitis and MSC play an important role in preventing the impairment of antioxidants defenses in inflamed colon.
authors.affiliation: Experimental Research Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, Porto Alegre, RS, CEP 90035-903, Brazil. fabygon85@gmail.com.|Graduate Program in Gastroenterology and Hepatology Sciences, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2400, Porto Alegre, RS, CEP 90035-903, Brazil. fabygon85@gmail.com.
authors.forename: Fabiany
authors.initials: F
authors.lastname: da Costa Gonçalves
authors.affiliation: Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90035-003, Brazil.
authors.forename: Mateus
authors.initials: M
authors.lastname: Grings
authors.affiliation: Experimental Research Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, Porto Alegre, RS, CEP 90035-903, Brazil.|Graduate Program in Gastroenterology and Hepatology Sciences, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2400, Porto Alegre, RS, CEP 90035-903, Brazil.
authors.forename: Natália Schneider
authors.initials: NS
authors.lastname: Nunes
authors.affiliation: Experimental Research Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
authors.forename: Fernanda Otesbelgue
authors.initials: FO
authors.lastname: Pinto
authors.affiliation: Experimental Research Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, Porto Alegre, RS, CEP 90035-903, Brazil.
authors.forename: Tuane Nerissa Alves
authors.initials: TN
authors.lastname: Garcez
authors.affiliation: Oral Pathology Department, School of Dentistry, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2492, Porto Alegre, RS, CEP 90035-007, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Visioli
authors.affiliation: Department of Biochemistry, Instituto de Ciências Básicas da Saúde, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2600, Porto Alegre, RS, CEP 90035-003, Brazil.
authors.forename: Guilhian
authors.initials: G
authors.lastname: Leipnitz
authors.affiliation: Experimental Research Center, Hospital de Clínicas de Porto Alegre, Ramiro Barcelos 2350, Porto Alegre, RS, CEP 90035-903, Brazil.|Graduate Program in Gastroenterology and Hepatology Sciences, Universidade Federal do Rio Grande do Sul, Ramiro Barcelos 2400, Porto Alegre, RS, CEP 90035-903, Brazil.
authors.forename: Ana Helena
authors.initials: AH
authors.lastname: Paz
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000502:physiology*; D002374:Catalase / Q000378:metabolism; D003092:Colitis / Q000139:chemically induced / Q000628:therapy*; D003106:Colon / Q000473:pathology; D016264:Dextran Sulfate; D005978:Glutathione / Q000378:metabolism; D015227:Lipid Peroxidation; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D008810:Mice, Inbred C57BL; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism
chemical_list: D000975:Antioxidants; D017392:Thiobarbituric Acid Reactive Substances; D016264:Dextran Sulfate; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione
keywords: Cell therapy; Colitis; Dextran sulfate sodium; Mesenchymal stem cell; Oxidative stress