title: Characterization of a novel SGNH-type esterase from Lactobacillus plantarum.
abstract: Lactic acid bacteria (LAB) are sources of a large variety of microbial ester hydrolases because they can produce a wide range of short-chain esters, phenolic alcohols, and fatty acids. Here, a novel SGNH-type esterase (LpSGNH1) from Lactobacillus plantarum WCFS1 was identified, functionally characterized, and immobilized for biotechnological applications. Homologs of LpSGNH1 are also found in many lactic acid bacteria (LAB) species. Biochemical features of LpSGNH1 were investigated using mass spectrometry, gel filtration chromatography, enzyme kinetics, fluorescence, and circular dichroism (CD) spectroscopy. LpSGNH1 were retained its activity under conditions that would be encountered during fermentations. Interestingly, LpSGNH1 exhibited the ability to act on a broad range of substrates including ketoprofen acetate, cefotaxime (CTX), and 7-aminocephalosporanic acid (7-ACA) as well as glucose pentaacetate, acetylxylan, and acetylalginate, which make LpSGNH1 a great candidate for extensive industrial applications. Furthermore, cross-linked enzyme aggregates of LpSGNH1 (CLEA-LpSGNH1) displayed recycling ability and thermal stability compared to free LpSGNH1, which could be useful for industrial applications. This work highlights the importance of LpSGNH1 in the preparation of commercial compounds, and LpSGNH1 can be used as a model system of SGNH esterases in lactic acid bacteria.
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, South Korea.
authors.forename: Yonggyu
authors.initials: Y
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, South Korea.
authors.forename: Bum Han
authors.initials: BH
authors.lastname: Ryu
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea.
authors.forename: Jimin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, South Korea.
authors.forename: Wanki
authors.initials: W
authors.lastname: Yoo
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea; Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, South Korea.
authors.forename: Deu Rae
authors.initials: DR
authors.lastname: An
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea.
authors.forename: Boo-Young
authors.initials: BY
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea.
authors.forename: Sena
authors.initials: S
authors.lastname: Kwon
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea.
authors.forename: Sojeong
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Molecular Cell Biology, Samsung Biomedical Research Institute, Sungkyunkwan University School of Medicine, Suwon 16419, South Korea.
authors.forename: Kyeong Kyu
authors.initials: KK
authors.lastname: Kim
authors.affiliation: Department of Chemistry, College of Natural Science, Sookmyung Women's University, Seoul 04310, South Korea. Electronic address: doohunkim@sookmyung.ac.kr.
authors.forename: T Doohun
authors.initials: TD
authors.lastname: Kim
mesh_terms: D000595:Amino Acid Sequence; D004800:Enzymes, Immobilized / Q000737:chemistry / Q000378:metabolism; D004950:Esterases / Q000737:chemistry* / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000201:enzymology*; D017421:Sequence Analysis
chemical_list: D004800:Enzymes, Immobilized; D004950:Esterases
keywords: Lactobacillus plantarum; LpSGNH1; SGNH esterase