title: Pulsed electromagnetic field at different stages of knee osteoarthritis in rats induced by low-dose monosodium iodoacetate: Effect on subchondral trabecular bone microarchitecture and cartilage degradation.
abstract: The aim of the study was to investigate the efficacy of pre-emptive, early, and delayed pulsed electromagnetic field (PEMF) treatment on cartilage and subchondral trabecular bone in knee osteoarthritis (OA) rats induced by low-dose monosodium iodoacetate (MIA). Seventy-five 12-week-old male Sprague-Dawley rats were assigned to five groups: OA (n = 30), pre-emptive PEMF (n = 10), early PEMF (n = 10), delayed PEMF (n = 10), and control (n = 15). Osteoarthritis was induced by injecting 0.2 mg MIA in rat's right knee joint. Control rats received a single sterile saline injection in the right knee. Male rats received pre-emptive (n = 10, day 0-end of week 4), early (n = 10, end of week 4-end of week 8), or delayed (n = 10, end of week 8-end of week 12) PEMF treatment (75 Hz, 1.6 mT). After 4, 8, and 12 weeks, rats were sacrificed at each time point and right knees were harvested. After sacrifice, micro-computed tomography, histology, and biomarker analyses were performed. We found pre-emptive PEMF treatment preserved subchondral trabecular bone microarchitecture and prevented subchondral bone loss in MIA-induced OA rat model. Early and delayed PEMF treatment maintained subchondral trabeculae. PEMF treatment increased bone and cartilage formation, and decreased bone and cartilage resorption. Pre-emptive and early PEMF treatment had moderate effects on cartilage degradation. Time point of treatment initiation is crucial for treating OA. PEMF might become a potential biophysical treatment modality for osteoarthritis. Bioelectromagnetics. 38:227-238, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.|Key Laboratory of Rehabilitation Medicine in Sichuan, Chengdu, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.|Key Laboratory of Rehabilitation Medicine in Sichuan, Chengdu, China.
authors.forename: Hongchen
authors.initials: H
authors.lastname: He
authors.affiliation: Institute for Disaster Management and Reconstruction of Sichuan University and Hong Kong Polytechnic University, Chengdu, China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.|Key Laboratory of Rehabilitation Medicine in Sichuan, Chengdu, China.
authors.forename: Yujing
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.|Key Laboratory of Rehabilitation Medicine in Sichuan, Chengdu, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Gao
authors.affiliation: Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.|Key Laboratory of Rehabilitation Medicine in Sichuan, Chengdu, China.
authors.forename: Pu
authors.initials: P
authors.lastname: Wang
authors.affiliation: Department of Rehabilitation Medicine, West China Hospital, Sichuan University, Chengdu, China.|Key Laboratory of Rehabilitation Medicine in Sichuan, Chengdu, China.|Institute for Disaster Management and Reconstruction of Sichuan University and Hong Kong Polytechnic University, Chengdu, China.
authors.forename: Chengqi
authors.initials: C
authors.lastname: He
mesh_terms: D000818:Animals; D000071556:Cancellous Bone / Q000648:ultrastructure*; D002358:Cartilage, Articular / Q000503:physiopathology*; D024042:Collagen Type I / Q000652:urine; D024043:Collagen Type II / Q000097:blood / Q000652:urine; D004195:Disease Models, Animal; D004574:Electromagnetic Fields; D019807:Iodoacetic Acid / Q000008:administration & dosage / Q000633:toxicity; D055909:Magnetic Field Therapy / Q000379:methods*; D008297:Male; D020370:Osteoarthritis, Knee / Q000139:chemically induced / Q000503:physiopathology / Q000628:therapy*; D015675:Osteocalcin / Q000097:blood; D010446:Peptide Fragments / Q000097:blood / Q000652:urine; D011347:Procollagen / Q000097:blood; D017207:Rats, Sprague-Dawley; D055114:X-Ray Microtomography
chemical_list: C000615613:CTX-I peptide, rat; C000615612:CTX-II peptide, rat; D024042:Collagen Type I; D024043:Collagen Type II; D010446:Peptide Fragments; D011347:Procollagen; C000615614:type II collagen N-propeptide, rat; D015675:Osteocalcin; D019807:Iodoacetic Acid
keywords: X-ray microtomography; cartilage; osteoarthritis; pulsed electromagnetic field; subchondral bone