title: Characterization of the CD4 and CD8 T-cell response in the QuantiFERON-TB Gold Plus kit.
abstract: OBJECTIVE
  QuantiFERON-TB Gold In-Tube (QFT-GIT, Qiagen, Hilden, Germany) is an interferon-γ (IFN-γ) release assay designed to detect latent tuberculosis infection (LTBI). Although QFT-GIT has several advantages (mainly that it is not affected by the Bacille Calmette-Guérin vaccination), it has a poor sensitivity in immune-compromised individuals as it involves an immune response-based detection. Recently, QuantiFERON-TB Gold Plus (QFT-Plus) assay has been proposed as a new generation of QFT-GIT. QFT-Plus includes two tubes, TB1 and TB2 with Mycobacterium tuberculosis antigens to elicit a specific immune response. TB1 contains peptides derived from the antigens 6kDa early secretory antigenic target (ESAT-6) and 10kDa culture filtrate protein (CFP-10) (TB-7.7, present in QFT-GIT, has been removed), and it is designed to induce a specific CD4 T-cell response. TB2 contains newly designed peptides stimulating IFN-γ production by both CD4 and CD8 T cells. The additional peptides for eliciting CD8 T-cell responses have been included to increase the sensitivity of the test for LTBI detection. The aim of the study was to evaluate specific CD4 and CD8 T-cell responses to the M. tuberculosis antigens contained within the QFT-Plus test by flow cytometry in individuals with active TB and LTBI.
  
  
  METHODS
  We enrolled 23 individuals with active TB and 30 individuals with LTBI. QFT-Plus assay and intracellular staining were performed. One million of peripheral blood mononuclear cells in 1ml of complete medium (RPMI 1640) were dispensed in QFT-Plus tubes. Following 16-24h stimulation, antigen-specific T cells were characterized by flow cytometry evaluating CD4, CD8, CD3 markers, and IFN-γ production. For statistical analysis, nonparametric tests were performed.
  
  
  RESULTS
  We found that CD4 T-cell responses were induced by both TB1 and TB2. Differently, the CD8 T-cell response was mainly induced by TB2 and was significantly higher than that induced by TB1 (p=0.01). The frequency of Mtb specific T-cells observed in individuals with active TB was significantly higher than in those with LTBI (p=0.04). Finally, TB2-specific CD8 T-cell responses in individuals with active TB were associated with high radiological severity of lung lesions and microbiological diagnosis (based on M. tuberculosis isolation in sputum culture).
  
  
  CONCLUSIONS
  This is the first characterization of CD4 and CD8 T-cell responses to QFT-Plus TB1 and TB2 tubes in individuals with active TB and LTBI enrolled in a low TB-endemic country such as Italy. We demonstrated that the increased sensitivity is a consequence of the ability of TB2 to induce a CD8 T-cell response which is mainly associated with active TB. This assay has the potential to be very useful in conditions of immune depression due to CD4 T-cell impairments.
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Elisa
authors.initials: E
authors.lastname: Petruccioli
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Teresa
authors.initials: T
authors.lastname: Chiacchio
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Ilaria
authors.initials: I
authors.lastname: Pepponi
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Vanini
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Rocco
authors.initials: R
authors.lastname: Urso
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Gilda
authors.initials: G
authors.lastname: Cuzzi
authors.affiliation: Emerging Bacterial Pathogens Unit, Division of Immunology and Infectious Diseases IRCCS, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Lucia
authors.initials: L
authors.lastname: Barcellini
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Fabrizio
authors.initials: F
authors.lastname: Palmieri
authors.affiliation: Emerging Bacterial Pathogens Unit, Division of Immunology and Infectious Diseases IRCCS, San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Daniela M
authors.initials: DM
authors.lastname: Cirillo
authors.affiliation: Scientific Direction, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy.
authors.forename: Giuseppe
authors.initials: G
authors.lastname: Ippolito
authors.affiliation: Translational Research Unit, Department of Epidemiology and Preclinical Research, National Institute for Infectious Diseases L Spallanzani (INMI), Rome, Italy. Electronic address: delia.goletti@inmi.it.
authors.forename: Delia
authors.initials: D
authors.lastname: Goletti
keywords: CD4; CD8; IGRA; QUANTIFERON PLUS; Tuberculosis