title: [Mechanisms of perioperative corneal abrasions: alterations in tear film proteome].
abstract: Perioperative corneal abrasion is an ophthalmic complication commonly found in patients underwent general anesthesia. In this study, correlations between development of corneal injury and proteomic changes in tear film during general anesthesia were examined using an animal (rabbit) model. Being started after 1-h anesthesia, the process of accumulation of pathological changes in the cornea unequivocally led clinically significant abrasions following 3-6 h of the narcosis. The corneal damage was associated with alterations in profiles of major proteins of the tear film. Analysis of the tear proteome pointed to depression of lachrymal glands function, and suggested serotransferrin, serum albumin and annexin A1 as potential tear markers of the complication. The tear film alterations included fast drop of total antioxidant activity and activity of superoxide dismutase, and decrease in interleukin-4 and increase in interleukin-6 content indicating development of oxidative and pro-inflammatory responses. These findings suggest antioxidant and anti-inflammatory therapy as prospective approach for prevention/treatment of perioperative corneal abrasions. The observed anesthesia-induced effects should be considered in any study of ocular surface diseases employing anesthetized animals.
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: E Yu
authors.initials: EY
authors.lastname: Zernii
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: O S
authors.initials: OS
authors.lastname: Gancharova
authors.affiliation: Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia.
authors.forename: I E
authors.initials: IE
authors.lastname: Ishutina
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: V E
authors.initials: VE
authors.lastname: Baksheeva
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: M O
authors.initials: MO
authors.lastname: Golovastova
authors.affiliation: Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia.
authors.forename: E I
authors.initials: EI
authors.lastname: Kabanova
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: M S
authors.initials: MS
authors.lastname: Savchenko
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: M V
authors.initials: MV
authors.lastname: Serebryakova
authors.affiliation: Skryabin Moscow State Academy of Veterinary Medicine and Biotechnology, Moscow, Russia.
authors.forename: L F
authors.initials: LF
authors.lastname: Sotnikova
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia; Sechenov First Moscow State Medical University, Institute of Molecular Medicine, Moscow, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Zamyatnin
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: P P
authors.initials: PP
authors.lastname: Philippov
authors.affiliation: Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: I I
authors.initials: II
authors.lastname: Senin
mesh_terms: D000818:Animals; D003315:Cornea / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D015352:Dry Eye Syndromes / Q000378:metabolism* / Q000473:pathology; D005136:Eye Proteins / Q000378:metabolism*; D005260:Female; D007765:Lacrimal Apparatus / Q000378:metabolism / Q000473:pathology; D008297:Male; D059035:Perioperative Period; D020543:Proteome / Q000378:metabolism*; D011817:Rabbits; D013666:Tears
chemical_list: D005136:Eye Proteins; D020543:Proteome; C042434:tear proteins
keywords: antioxidant activity; corneal abrasion; dry eye; general anesthesia; pro-inflammatory response; tear proteome