title: HYSYS: have you swapped your samples?
abstract: The application of a genomics assay to samples from a cohort is a frequently applied experimental design in cancer genomics studies. The collection and analysis of cancer sequencing data in the clinical setting is an elaborate process that may involve consenting patients, obtaining possibly-multiple DNA samples, sequencing and analysis. Many of these steps are manual. At any stage mistakes can occur that cause a DNA sample to be labelled incorrectly. However, there is a paucity of methods in the literature to identify such swaps specifically in cancer studies.
  
  
  
  Here, we introduce a simple method, HYSYS, to estimate the relatedness of samples and test for sample swaps and contamination. The test uses the concordance of homozygous SNPs between samples. The method is motivated by the observation that homozygous germline population variants rarely change in the disease and are not affected by loss of heterozygosity. Our tools include visualization and a testing framework to flag possible sample swaps. We demonstrate the utility of this approach on a small cohort.
  
  
  
  http://github.com/PapenfussLab/HaveYouSwappedYourSamples.
  
  
  
  papenfuss@wehi.edu.au.
  
  
  
  Supplementary data are available at Bioinformatics online.
authors.affiliation: Bioinformatics Division, The Walter & Eliza Hall Institute, Parkville 3052, Australia.|Computing and Information Systems.|Peter MacCallum Cancer Centre, Melbourne 3000, Australia.
authors.forename: Jan
authors.initials: J
authors.lastname: Schröder
authors.affiliation: Bioinformatics Division, The Walter & Eliza Hall Institute, Parkville 3052, Australia.|Department of Medical Biology, The University of Melbourne, Melbourne 3010, Australia.
authors.forename: Vincent
authors.initials: V
authors.lastname: Corbin
authors.affiliation: Bioinformatics Division, The Walter & Eliza Hall Institute, Parkville 3052, Australia.|Department of Medical Biology, The University of Melbourne, Melbourne 3010, Australia.|Peter MacCallum Cancer Centre, Melbourne 3000, Australia.|Sir Peter MacCallum Department of Oncology, University of Melbourne, Melbourne 3010, Australia.
authors.forename: Anthony T
authors.initials: AT
authors.lastname: Papenfuss
mesh_terms: D059018:DNA Contamination; D023281:Genomics / Q000379:methods*; D006801:Humans; D009369:Neoplasms / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide; D011786:Quality Control; D017422:Sequence Analysis, DNA / Q000379:methods; D012984:Software