title: The effects of cadmium on the biochemical and physiological parameters of Eruca sativa.
abstract: In this study, Eruca sativa (Rocket) seedlings were treated with different cadmium (Cd) concentrations (0, 150, 300 and 450 μg ∙ g-1). The effects of Cd on lipid peroxidation, enzymatic (APx, CAT, GPX, SOD) and non-enzymatic antioxidants (total ascorbate, dehydroascorbate, ascorbate, non-protein thiol), fresh and dry masses, water content were determined. Also, Cd content of the leaves and the roots were analysed. The highest cadmium accumulation of leaves was at 450 μg ∙ g-1Cd treatment and the accumulation was 2.62 times greater than those in the roots. The translocation factor was 3.89 at 300 μg ∙ g-1Cd treatment. Cd treatments caused decreases of fresh, dry mass and water content of leaves and roots. Malondialdehyde content, which is an index of lipid peroxidation, was increased in proportion with the increase in Cd. While there was not change in the activity of GPX according to control, a decrease in activities of SOD, CAT and APX were observed with the increase of cadmium concentration. Although a significant increase in the amounts of non-protein thiol groups and proline were observed in 450 μg ∙ g-1Cd treated plants, Cd did not lead to a significant change in AsA, DHA and total AsA contents. According to the results of the research, E. sativa may be a Cd hyperaccumulator plant and we suggest that the plant may be a candidate plant for remediation of Cd-contaminated soil.
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, University of Ondokuz mayis , Samsun , Turkey.
authors.forename: Yasemin Ozdener
authors.initials: YO
authors.lastname: Kompe
authors.affiliation: Department of Biology, Faculty of Arts and Sciences, University of Ondokuz mayis , Samsun , Turkey.
authors.forename: Ahmet
authors.initials: A
authors.lastname: Sagiroglu
mesh_terms: D000975:Antioxidants / Q000378:metabolism; D060387:Ascorbate Peroxidases / Q000187:drug effects / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D019607:Brassicaceae / Q000187:drug effects* / Q000201:enzymology / Q000378:metabolism; D002104:Cadmium / Q000494:pharmacology*; D002374:Catalase / Q000187:drug effects / Q000378:metabolism; D003683:Dehydroascorbic Acid / Q000378:metabolism; D015227:Lipid Peroxidation / Q000187:drug effects; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D009195:Peroxidase / Q000187:drug effects / Q000378:metabolism; D018515:Plant Leaves / Q000187:drug effects* / Q000378:metabolism; D018517:Plant Roots / Q000187:drug effects* / Q000378:metabolism; D011392:Proline / Q000187:drug effects / Q000378:metabolism; D013438:Sulfhydryl Compounds / Q000378:metabolism; D013482:Superoxide Dismutase / Q000187:drug effects / Q000378:metabolism; D014867:Water / Q000378:metabolism
chemical_list: D000975:Antioxidants; D013438:Sulfhydryl Compounds; D002104:Cadmium; D014867:Water; D008315:Malondialdehyde; D011392:Proline; C121048:guaiacol peroxidase; D060387:Ascorbate Peroxidases; D002374:Catalase; D009195:Peroxidase; D013482:Superoxide Dismutase; D001205:Ascorbic Acid; D003683:Dehydroascorbic Acid
keywords: Eruca sativa; cadmium; hyperaccumulator; oxidative stress