title: Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa2EDTA chelation therapy.
abstract: The CaNa2EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa2EDTA chelation therapy. DOLE demonstrated pronounced radical scavenging activity in concentrations ≥1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 ± 14.26) compared to controls (6.0 ± 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 ± 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 ± 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 ± 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.
authors.affiliation: Faculty of Pharmacy, Department of Physiology, University of Belgrade, Serbia. Electronic address: andrea@pharmacy.bg.ac.rs.
authors.forename: Andrea
authors.initials: A
authors.lastname: Čabarkapa
authors.affiliation: Biomedical Research, R&D Institute, Galenika a.d., Belgrade, Serbia. Electronic address: ddekan@sezampro.rs.
authors.forename: Dragana
authors.initials: D
authors.lastname: Dekanski
authors.affiliation: Faculty of Pharmacy, Department of Physiology, University of Belgrade, Serbia. Electronic address: lada@pharmacy.bg.ac.rs.
authors.forename: Lada
authors.initials: L
authors.lastname: Živković
authors.affiliation: Institute of Occupational Health of Serbia, Belgrade, Serbia. Electronic address: mirmilcab@gmail.com.
authors.forename: Mirjana
authors.initials: M
authors.lastname: Milanović-Čabarkapa
authors.affiliation: Institute for Nuclear Research "Vinca", Laboratory for Radiobiology and Molecular Genetics, University of Belgrade, Serbia. Electronic address: vladanbajic@gmail.com.
authors.forename: Vladan
authors.initials: V
authors.lastname: Bajić
authors.affiliation: Faculty of Pharmacy, Department of Physiology, University of Belgrade, Serbia. Electronic address: dijana@pharmacy.bg.ac.rs.
authors.forename: Dijana
authors.initials: D
authors.lastname: Topalović
authors.affiliation: Department of Odontostomatologic and Specialized Clinical Science, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy. Electronic address: f.giampieri@univpm.it.
authors.forename: Francesca
authors.initials: F
authors.lastname: Giampieri
authors.affiliation: Department of Odontostomatologic and Specialized Clinical Science, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy. Electronic address: m.gasparrini@univpm.it.
authors.forename: Massimiliano
authors.initials: M
authors.lastname: Gasparrini
authors.affiliation: Department of Odontostomatologic and Specialized Clinical Science, Faculty of Medicine, Polytechnic University of Marche, Ancona, Italy. Electronic address: m.a.battino@univpm.it.
authors.forename: Maurizio
authors.initials: M
authors.lastname: Battino
authors.affiliation: Faculty of Pharmacy, Department of Physiology, University of Belgrade, Serbia. Electronic address: bilja22@pharmacy.bg.ac.rs.
authors.forename: Biljana
authors.initials: B
authors.lastname: Spremo-Potparević
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000008:administration & dosage; D002614:Chelating Agents / Q000008:administration & dosage*; D015913:Chelation Therapy; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D006801:Humans; D007854:Lead / Q000633:toxicity*; D007855:Lead Poisoning / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects* / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D009784:Occupational Diseases / Q000097:blood / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D016273:Occupational Exposure / Q000009:adverse effects; D031658:Olea / Q000737:chemistry*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000008:administration & dosage*; D018515:Plant Leaves / Q000737:chemistry
chemical_list: D000975:Antioxidants; D002614:Chelating Agents; D010936:Plant Extracts; D007854:Lead
keywords: Chelation; DNA damage; Dry olive leaf extract (DOLE); Lead (Pb); Oxidative stress