title: MicroRNA-182 targets FOXF2 to promote the development of triple-negative breast cancer.
abstract: To explore the function of microRNA-182 (miR-182) on MCF7 and MDA-MB-231 cells behaviors, and possible mechanisms of triple-negative breast cancer (TNBC) development. Totally, 30 TNBC patients were enrolled to investigate the correlation between miR-182 expression and TNBC clinical indicators. miR-182 expression in TNBC tissues was measured by qRT-PCR, followed by bioinformatics methods and luciferase reporter assay to investigate whether FOXF2 was a direct target of miR-182. Besides, miR-182 mimics were transfected into MCF7 cells while miR-182 inhibitor into MDA-MB-231 cells, followed by cell proliferation and migration detection. miR-182 expression was significantly correlated with TNBC clinical indicators, such as lymph node metastasis TNM (stage III), intravascular cancer emboli and TNBC recurrence and metastasis. miR-182 expression was significantly higher in TNBC tissues than that in matched normal tissues, and was significantly higher in MDA-MB-231 cells than that in MCF7 cells. miR-182 knockdown inhibited the proliferation and migration of MDA-MB-231 cells while miR-182 overexpression markedly promoted the proliferation and migration of MCF7 cells. Besides, FOXF2 was identified as a direct target of miR-182. Our findings indicate that miR-182 may promote cell proliferation and migration in TNBC possible via down-regulation of FOXF2. miR-182 may serve as a potential target in TNBC treatment.
authors.forename: J
authors.initials: J
authors.lastname: Yu
authors.forename: W
authors.initials: W
authors.lastname: Shen
authors.forename: B
authors.initials: B
authors.lastname: Gao
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: J
authors.initials: J
authors.lastname: Xu
authors.forename: B
authors.initials: B
authors.lastname: Gong
mesh_terms: D045744:Cell Line, Tumor; D049109:Cell Proliferation; D015536:Down-Regulation; D051858:Forkhead Transcription Factors / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D009364:Neoplasm Recurrence, Local; D064726:Triple Negative Breast Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C486413:FOXF2 protein, human; D051858:Forkhead Transcription Factors; D035683:MicroRNAs; C538984:Mirn182 microRNA, human
keywords: FOXF2; microRNA-182; proliferation migration.; triple-negative breast cancer