title: [Determination of 1-hydroxy-2-butanone in urine by gas chromatography].
abstract: Objective:To establish a method for the determination of 1-hydroxy-2-butanone in urine by gas chromatography.Methods:Urine samples were acidified with hydrochloric acid, and then stored in the refrigerator . After thawing under natural conditions, urine volume of 2.0 ml was extracted by C18solid phase, using methanol volume of 2.0 ml elution, detected by gas chromatography with FID detector and quantified by external standard method.Results:The measurement range of method is 0.986 ~32.88 μg/ml, correlation coefficient (r) =0.999 7, detection limit is 0.13 μg/ml; The recovery is 88.1% ~92.2% ; The precision is 1.4% ~3.2%.Conclusion:This method has a high sensitivity and simple processing. All technical indicators can meet the requirements of the determination method.
authors.affiliation: Henan Provincial Institute of Occupational Health, Zhengzhou 450052, China.
authors.forename: C Y
authors.initials: CY
authors.lastname: Wang
authors.forename: Y Y
authors.initials: YY
authors.lastname: Lu
authors.forename: W H
authors.initials: WH
authors.lastname: Qin
mesh_terms: D002074:Butanones / Q000032:analysis*; D002849:Chromatography, Gas; D057230:Limit of Detection
chemical_list: D002074:Butanones; C487002:1-hydroxy-2-butanone