title: Light-sheet-based 2D light scattering cytometry for label-free characterization of senescent cells.
abstract: A light-sheet-based 2D light scattering cytometer is developed for label-free characterization of senescent cells. The light-sheet provides an illumination beam with controlled thickness for single cell excitation, and 2D light scattering patterns are obtained by using a defocused imaging method. The principle of this cytometer is validated by distinguishing microspheres with submicron resolution. Automatic classification of senescent and normal cells is achieved at single cell level by using the support vector machine (SVM) algorithm, where a sensitivity of 89.1% and a specificity of 96.4% are obtained. Our results suggest that the light-sheet-based 2D light scattering label-free cytometry has the capability to perform size differentiation of beads with submicron resolution and to classify different groups of cells without fluorescent labeling, showing the potential for clinical diagnosis of senescence-related diseases.
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Meiai
authors.initials: M
authors.lastname: Lin
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Qiao
authors.affiliation: Key Laboratory of Experimental Teratology (Ministry of Education); Department of Molecular Medicine and Genetics, School of Medicine, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Key Laboratory of Experimental Teratology (Ministry of Education); Department of Molecular Medicine and Genetics, School of Medicine, Shandong University, Jinan, Shandong, 250012, China.
authors.forename: Changshun
authors.initials: C
authors.lastname: Shao
authors.affiliation: Institute of Biomedical Engineering, School of Control Science and Engineering, Shandong University, Jinan, Shandong, 250061, China.
authors.forename: Xuantao
authors.initials: X
authors.lastname: Su
keywords: (120.3890) Medical optics instrumentation; (120.5820) Scattering measurements; (170.1530) Cell analysis; (170.4580) Optical diagnostics for medicine