title: Efficacy of a new implant-abutment connection to minimize microbial contamination: anin vitrostudy.
abstract: OBJECTIVE
  The aim of the present study is to evaluate the effectiveness of Ditron implants abutment connection (IAC) to sealing the gap between two pieces.
  
  
  METHODS
  To identify the efficacy of a new IAC, the passage of genetically modified bacteria across IAC was evaluated. A total of five Ditron Implants were used. All implants were immerged in a bacterial culture for forty-eight hours and then bacteria amount was measured inside and outside IAC with Real-time PCR. Bacterial quantification was performed by Real-Time Polymerase Chain Reaction using the absolute quantification with the standard curve method.
  
  
  RESULTS
  In all the tested implants, bacteria were found in the inner side, with a median percentage of 1.35%. The analysis revealed that, in untreated implants, bacteria grew (internally and externally). Moreover, the difference between outer and inner bacteria concentration was statistically significant at each time point.
  
  
  CONCLUSIONS
  Ditron Implant IAC (MPI, Ditron Dental, Israel) is efficacy in reducing bacterial leakage.
authors.affiliation: Maxillo-Facial and Dental Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.
authors.forename: G E
authors.initials: GE
authors.lastname: Mancini
authors.affiliation: Maxillo-Facial and Dental Unit, Fondazione Cà Granda IRCCS Ospedale Maggiore Policlinico, Milan, Italy.
authors.forename: A B
authors.initials: AB
authors.lastname: Gianni'
authors.affiliation: Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Cura
authors.affiliation: Department of Oral Rehabilitation, Tel-Aviv University, Tel-Aviv, Israel.
authors.forename: Z
authors.initials: Z
authors.lastname: Ormanier
authors.affiliation: Department of Morphology, Surgery and Experimental Medicine, University of Ferrara, Ferrara, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Carinci
keywords: bone resorption; implant-abutment connection; microbiological leakage; peri-implantitis