title: Chaetomium globosum CDW7, a potential biological control strain and its antifungal metabolites.
abstract: Screening for endophytic fungi with antifungal activity is an effective strategy for the discovery of novel biopesticides. Our previous work indicated that Chaetomium globosum CDW7, an endophyte from Ginkgo biloba, exhibited strong inhibitory activity against plant pathogenic fungi in vitro. In this study, we evaluated the CDW7 strain for its antifungal activities against nine phytopathogenic fungi and its biocontrol potential against rape sclerotinia rot caused by Sclerotinia sclerotiorum. The fermentation broth of CDW7 could successfully inhibit disease development in S. sclerotiorum-infected rape in vivo with 57.8% protective efficiency, which is comparable to that of carbendazim (59.8%) at 250 μg mL-1. The fermentation broth also expressed significant activity stability when exposed to 60°C and UV illumination, or when stored at 4°C. Furthermore, we found that 10% fermentation broth can promote the germination and growth of rape seedlings. Followed by the bioassay-guided approach, seven known metabolites were isolated and identified by spectroscopic analyses. Among them, chaetoglobosin A and D exhibited inhibitory activity against S. sclerotiorum with IC50 values of 0.35 and 0.62 μg mL-1, respectively, compared with carbendazim (0.17 μg mL-1). Therefore, our study demonstrated that CDW7 is a promising biocontrol fungus against S. sclerotiorum in agriculture.
authors.affiliation: College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R. China.
authors.forename: Shuang-Shuang
authors.initials: SS
authors.lastname: Zhao
authors.affiliation: College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R. China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R. China.
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Cao
authors.affiliation: College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Xiao
authors.affiliation: College of Plant Protection, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing Agricultural University, Nanjing 210095, P.R. China.|Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing 210095, P.R. China.
authors.forename: Yong-Hao
authors.initials: YH
authors.lastname: Ye
mesh_terms: D000898:Antibiosis; D000935:Antifungal Agents / Q000302:isolation & purification / Q000494:pharmacology*; D001203:Ascomycota / Q000187:drug effects*; D001562:Benzimidazoles / Q000494:pharmacology; D061046:Biological Control Agents / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology*; D002219:Carbamates / Q000494:pharmacology; D002597:Chaetomium / Q000378:metabolism*; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D020441:Ginkgo biloba / Q000382:microbiology; D026121:Indole Alkaloids / Q000494:pharmacology; D020128:Inhibitory Concentration 50
chemical_list: D000935:Antifungal Agents; D001562:Benzimidazoles; D061046:Biological Control Agents; D002219:Carbamates; D003470:Culture Media; D026121:Indole Alkaloids; C019290:chaetoglobosins; C006698:carbendazim
keywords: Sclerotinia sclerotiorum; antifungal activity; biological control; endophytic fungi; secondary metabolites