title: Structure-Based Design and Synthesis of a New Phenylboronic-Modified Affinity Medium for Metalloprotease Purification.
abstract: Metalloproteases are emerging as useful agents in the treatment of many diseases including arthritis, cancer, cardiovascular diseases, and fibrosis. Studies that could shed light on the metalloprotease pharmaceutical applications require the pure enzyme. Here, we reported the structure-based design and synthesis of the affinity medium for the efficient purification of metalloprotease using the 4-aminophenylboronic acid (4-APBA) as affinity ligand, which was coupled with Sepharose 6B via cyanuric chloride as spacer. The molecular docking analysis showed that the boron atom was interacting with the hydroxyl group of Ser176 residue, whereas the hydroxyl group of the boronic moiety is oriented toward Leu175 and His177 residues. In addition to the covalent bond between the boron atom and hydroxyl group of Ser176, the spacer between boronic acid derivatives and medium beads contributes to the formation of an enzyme-medium complex. With this synthesized medium, we developed and optimized a one-step purification procedure and applied it for the affinity purification of metalloproteases from three commercial enzyme products. The native metalloproteases were purified to high homogeneity with more than 95% purity. The novel purification method developed in this work provides new opportunities for scientific, industrial and pharmaceutical projects.
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China. lshywln@163.com.
authors.forename: Shangyong
authors.initials: S
authors.lastname: Li
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China. wlnwfllsy@163.com.
authors.forename: Linna
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Bioinformatics and Medical Engineering, School of Electrical and Information Engineering, Jiangsu University of Technology, Changzhou 213000, China. ximing.xu@jsut.edu.cn.
authors.forename: Ximing
authors.initials: X
authors.lastname: Xu
authors.affiliation: Laboratory of Oncology and Molecular Endocrinology, CHUL Research Center (CHUQ) and Laval University, 2705 Boulevard Laurier, Ste-Foy, Ville de Québec, QC G1V 4G2, Canada. sheng-xiang.lin@crchul.ulaval.ca.
authors.forename: Shengxiang
authors.initials: S
authors.lastname: Lin
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China. wangyj@ysfri.ac.cn.
authors.forename: Yuejun
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China. haojh@ysfri.ac.cn.|Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. haojh@ysfri.ac.cn.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Hao
authors.affiliation: Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266071, China. sunmi0532@yahoo.com.|Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. sunmi0532@yahoo.com.
authors.forename: Mi
authors.initials: M
authors.lastname: Sun
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry*; D001895:Boron / Q000737:chemistry; D001897:Boronic Acids / Q000737:chemistry*; D002846:Chromatography, Affinity / Q000379:methods*; D020650:Combinatorial Chemistry Techniques / Q000379:methods; D006863:Hydrogen-Ion Concentration; D008024:Ligands; D045726:Metalloproteases / Q000737:chemistry*; D062105:Molecular Docking Simulation / Q000379:methods; D012685:Sepharose / Q000737:chemistry
chemical_list: C000599649:4-aminophenylboronic acid; D000814:Aniline Compounds; D001897:Boronic Acids; D008024:Ligands; D012685:Sepharose; D045726:Metalloproteases; D001895:Boron
keywords: adsorption analysis; affinity purification; aminophenylboronic acid; metalloprotease; molecular docking