title: Screening for antitumor activity of various plant extracts on HeLa and C 4-I cell lines.
abstract: OBJECTIVE
  Cancer is a long process that leads the organism to death and is associated with the normal cells acquiring the ability to divide permanently. Nowadays, the use of natural products in cancer therapy has a great importance. In addition, working with plants that are endemic to Turkey and determining the biological activities of these plant extracts, is extremely important due to the potential for new drug development. There is no comparative study available in the literature on the antitumor effects of Colchicum sanguicolle, a new found species of the genus Colchicum in Turkey, Crateagus microphylla, of the genus Crateagus and Centaurea antiochia of the genus Centaurea. In this study, we tried to demonstrate the antitumor effect of these plant extracts on HeLa and C 4-1 cells.
  
  
  METHODS
  Five different doses (0.001, 0.01, 0.05, 0.25 and 0.5 mg/ml) of the three plant types were prepared and applied for 24, 48 and 72 hrs on the cervical cancer derived cell lines. Subsequently, the growth rate was evaluated with the mitochondrial dehydrogenase enzyme method.
  
  
  RESULTS
  Colchicum sanguicolle extracts showed the most effective antitumor activity. For the Colchicum sanguicolle extract, the IC50 dose for HeLa cells was 0.01 mg/ml at 48 hrs, while for the C-4 I cells it was 0.001 mg/ml at 48 hrs. These results showed that C-4 I cells were more sensitive to the Colchicum sanguicolle extracts. Conclus?on: The results of from this study regarding the antitumor effect of plant extracts of endemic varieties of Turkey may have an important place in design and development of anticancer drugs and would make contributions to other studies to be conducted in this area.
authors.affiliation: Department of Biology, Istanbul University, Faculty of Science, Istanbul, Turkey.
authors.forename: Gul
authors.initials: G
authors.lastname: Ozcan
authors.forename: Ozlem
authors.initials: O
authors.lastname: Dagdeviren Ozsoylemez
authors.forename: Gizem
authors.initials: G
authors.lastname: Akman
authors.forename: Walid
authors.initials: W
authors.lastname: Khalilia
authors.forename: Beliz
authors.initials: B
authors.lastname: Tezel Yetiz
authors.forename: Ali
authors.initials: A
authors.lastname: Karagoz
authors.forename: Gulay
authors.initials: G
authors.lastname: Melikoglu
authors.forename: Sezin
authors.initials: S
authors.lastname: Anil
authors.forename: Sukran
authors.initials: S
authors.lastname: Kultur
authors.forename: Nurhayat
authors.initials: N
authors.lastname: Sutlupinar
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D014408:Biomarkers, Tumor / Q000378:metabolism; D049109:Cell Proliferation / Q000187:drug effects; D048430:Cell Shape / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003079:Colchicum / Q000737:chemistry* / Q000145:classification; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005260:Female; D006367:HeLa Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D024101:Mitochondrial Proteins / Q000378:metabolism; D010088:Oxidoreductases / Q000378:metabolism; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D010946:Plants, Medicinal; D013997:Time Factors; D014421:Turkey; D002583:Uterine Cervical Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D014408:Biomarkers, Tumor; D024101:Mitochondrial Proteins; D010936:Plant Extracts; D010088:Oxidoreductases