title: MS for investigation of time-dependent protein adsorption on surfaces in complex biological samples.
abstract: OBJECTIVE
  This study aims at developing a nondestructive way for investigating protein adsorption on surfaces such as biomaterials using mass spectrometry.
  
  
  METHODS
  Ventricular cerebrospinal fluid in contact with poly carbonate membranes were used as adsorption templates and on-surface enzymatic digestion was applied to desorb proteins and cleave them into peptides. Mass spectrometric analysis provided both protein identification and determination of protein specific adsorption behavior.
  
  
  RESULTS
  In general, the adsorption increased with incubation time but also protein-specific time-resolved adsorption patterns from the complex protein solution were discovered.
  
  
  CONCLUSIONS
  The method developed is a promising tool for the characterization of biofouling, which sometimes causes rejection and encapsulation of implants and can be used as complement to other surface analytical techniques.
authors.affiliation: Department of Chemistry-BMC, Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden; Department of Chemistry-BMC, Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden.
authors.forename: Torgny
authors.initials: T
authors.lastname: Undin
authors.affiliation: Department of Chemistry-BMC, Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden; Department of Chemistry-BMC, Science for Life Laboratory, Uppsala University, PO Box 599, SE-751 24 Uppsala, Sweden.
authors.forename: Sara Bergström
authors.initials: SB
authors.lastname: Lind
authors.affiliation: Department of Engineering Sciences, Uppsala University, PO Box 534, SE-751 21 Uppsala, Sweden; Department of Engineering Sciences, Uppsala University, PO Box 534, SE-751 21 Uppsala, Sweden.
authors.forename: Andreas P
authors.initials: AP
authors.lastname: Dahlin
keywords: MS; on-surface digestion; polycarbonate membrane; time-resolved protein adsorption; ventricular cerebrospinal fluid