title: Physicochemical characterization and immunological properties of Pichia pastoris based HPV16L1 and 18L1 virus like particles.
abstract: There continues to be an urgent need for cost-effective prophylaxis for HPV-associated cancers in socio-economically underdeveloped nations. Presently HPV vaccines, which are commercially available, are adjuvanted virus-like particles (VLPs) expressed from various recombinant expression systems. They have been characterized by different methods as safe, pure, and potent HPV vaccine antigens. We cloned and expressed L1 proteins of HPV16 & 18 in Pichia pastoris and tested their immunogenicity. We observed that HPVL1 proteins (16L1 and 18L1) are expressed in Pichia pastoris at high levels. Critical physicochemical parameters of these HPV recombinant L1 proteins were characterized by SDS PAGE, western blotting, peptide mapping, glycosylation pattern, mass spectrometry, host cell DNA and protein analysis, electron microscopy, and immunogenicity analysis. These data establish a blueprint of HPV recombinant protein antigens for standardizing & developing an alternative high-quality, cost-effective vaccine for HPV as well as similar recombinant protein-based vaccines.
authors.affiliation: Department of Virology & Biotechnology, Vaccine Technology Centre, Zydus Biologics Compound, Sarkhej Bavla NH No 8 A, Changodar, Ahmedabad, 382213, Gujarat, India; Amity Institute of Virology and Immunology, Amity University, Noida, 201303, Uttar Pradesh, India. Electronic address: drgaurav123@gmail.com.
authors.forename: Gaurav
authors.initials: G
authors.lastname: Gupta
authors.affiliation: Department of Virology & Biotechnology, Vaccine Technology Centre, Zydus Biologics Compound, Sarkhej Bavla NH No 8 A, Changodar, Ahmedabad, 382213, Gujarat, India. Electronic address: recafra@gmail.com.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Glueck
authors.affiliation: Amity Institute of Virology and Immunology, Amity University, Noida, 201303, Uttar Pradesh, India. Electronic address: nrishi@amity.edu.
authors.forename: Narayan
authors.initials: N
authors.lastname: Rishi
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D015153:Blotting, Western; D036022:Capsid Proteins / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D055598:Chemical Phenomena; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006031:Glycosylation; D007074:Immunoglobulin G / Q000276:immunology; D008807:Mice, Inbred BALB C; D009856:Oncogene Proteins, Viral / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D030361:Papillomavirus Infections / Q000276:immunology / Q000821:virology; D053918:Papillomavirus Vaccines / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D010843:Pichia / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000276:immunology* / Q000378:metabolism; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D014611:Vaccination; D014771:Virion / Q000235:genetics / Q000276:immunology* / Q000378:metabolism
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D007074:Immunoglobulin G; D009856:Oncogene Proteins, Viral; D053918:Papillomavirus Vaccines; D011994:Recombinant Proteins; C063317:L1 protein, Human papillomavirus type 16; C480565:L1 protein, Human papillomavirus type 18
keywords: HPV vaccine; HPV16L1/18L1 antigens; Immunogenicity; Physicochemical properties; Pichia pastoris; Virus like particles