title: Autophagy induction reduces telomerase activity in HeLa cells.
abstract: Autophagy is a cellular homeostatic process whereby damaged proteins and organelles are encapsulated into double membrane vesicles, called autophagosomes, for lysosomal digestion. Beclin1 plays a key role in the initial steps of autophagosome formation. In this study, we evaluated the effect of Beclin 1 overexpression in induction of autophagy and the relationship between autophagy induction and telomerase activity in HeLa cells. We found that overexpression of Beclin 1 in HeLa cells leads to autophagosome formation as shown by intracellular autophagosomal marker LC3-II staining. Expression of Beclin1 reduced telomerase activity for about 100 fold compared with the control while it did not affect TERT expression level. The results of cell cycle analysis indicated that the cell cycle and proliferation progressed normally up to 48h post-transfection. Understanding the role of autophagy induction and telomerase in the pathophysiology of aging and human cancer reveal new strategies that hold much promise for intervention and therapeutic uses.
authors.affiliation: Department of Animal Biology, Faculty of Biological Science, Kharazmi University, Tehran, 13169-43551, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Taji
authors.affiliation: Department of Animal Biology, Faculty of Biological Science, Kharazmi University, Tehran, 13169-43551, Iran.
authors.forename: Homa Mohseni
authors.initials: HM
authors.lastname: Kouchesfahani
authors.affiliation: WHO Collaborating Center for Reference and Research on Rabies, Pasteur Institute of Iran, Tehran, 13169-43551, Iran.
authors.forename: Farzaneh
authors.initials: F
authors.lastname: Sheikholeslami
authors.affiliation: Cellular and Molecular Research Center (CMRC), Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences (AJUMS), Ahvaz, 61357-15794, Iran; Department of Biochemistry, University of Alberta, Edmonton, Alberta, T6G 2E1, Canada.
authors.forename: Bizhan
authors.initials: B
authors.lastname: Romani
authors.affiliation: Hepatitis and AIDS Dept., Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Kazem
authors.initials: K
authors.lastname: Baesi
authors.affiliation: Medical Lab Technology Department, School of Allied Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Rouhollah
authors.initials: R
authors.lastname: Vahabpour
authors.affiliation: Department of Hematology, Allied Medical School, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Mahdi
authors.initials: M
authors.lastname: Edalati
authors.affiliation: Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Iran.
authors.forename: Ladan
authors.initials: L
authors.lastname: Teimoori-Toolabi
authors.affiliation: Hepatitis and AIDS Dept., Pasteur Institute of Iran, Tehran, Iran.
authors.forename: Ehsan Ollah
authors.initials: EO
authors.lastname: Jazaeri
authors.affiliation: Hepatitis and AIDS Dept., Pasteur Institute of Iran, Tehran, Iran. Electronic address: Asghar.abdoli7@gmail.com.
authors.forename: Asghar
authors.initials: A
authors.lastname: Abdoli
mesh_terms: D000071182:Autophagosomes / Q000378:metabolism*; D001343:Autophagy; D000071186:Beclin-1 / Q000096:biosynthesis* / Q000235:genetics; D006367:HeLa Cells; D006801:Humans; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D019098:Telomerase / Q000235:genetics / Q000378:metabolism*
chemical_list: C491997:BECN1 protein, human; D000071186:Beclin-1; C477012:MAP1LC3A protein, human; D008869:Microtubule-Associated Proteins; D019098:Telomerase
keywords: Aging; Autophagy; Beclin 1; HeLa cell; Telomerase activity