title: Immunomodulatory effects of Pseudostellaria heterophylla (Miquel) Pax on regulation of Th1/Th2 levels in mice with atopic dermatitis.
abstract: Pseudostellaria heterophylla (PH) has various pharmacological effects that include immunologic enhancement and anti‑oxidation. However, it remains unclear whether PH exerts beneficial effects in dermatological diseases. The present study examined the effects of PH on a 2,4-dinitrochlorobenzene (DNCB)‑induced atopic dermatitis (AD) mouse model and elucidated its underlying mechanism of action. PH extract (1 and 100 mg/ml) was applied topically to DNCB-treated dorsal skin of mice every day for 11 days. The immunomodulatory effects of PH were evaluated by measuring skin thickness, mast cell infiltration, serum levels of immunoglobulin E (IgE), and mRNA expression levels of T helper (h)1/Th2 and pro‑inflammatory cytokines in dorsal skin. In addition, cluster of differentiation (CD)4+ T cells were detected in dorsal skin by immunohistochemistry. Topical application of PH significantly reduced the thickness of dermis, epidermis and serum IgE production compared with the DNCB group. PH treatment inhibited infiltration of inflammatory cells, including mast cells and CD4+ T cells, and suppressed the mRNA expression levels of cytokines (interferon‑γ, interleukin‑4, ‑6, ‑8 and ‑1β, and tumor necrosis factor‑α) associated with the immune response. Furthermore, PH treatment significantly downregulated the protein expression levels of nuclear factor‑κB, phosphorylated inhibitor of κBα and mitogen‑activated protein kinases. The results suggested that PH may be a potential therapeutic strategy for the treatment of AD via the modulation of Th1 and Th2 levels.
authors.affiliation: Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: You Yeon
authors.initials: YY
authors.lastname: Choi
authors.affiliation: Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Mi Hye
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Department of Korean Pathology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Kwang Seok
authors.initials: KS
authors.lastname: Ahn
authors.affiliation: Department of Korean Pharmacology, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Jae-Young
authors.initials: JY
authors.lastname: Um
authors.affiliation: Cancer Preventive Material Development Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Seok-Geun
authors.initials: SG
authors.lastname: Lee
authors.affiliation: Department of Convergence Korean Medical Science, College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
authors.forename: Woong Mo
authors.initials: WM
authors.lastname: Yang
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000188:drug therapy / Q000276:immunology / Q000473:pathology*; D005260:Female; D051550:I-kappa B Kinase / Q000378:metabolism; D007073:Immunoglobulin E / Q000097:blood; D034921:Lamin Type B / Q000235:genetics / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B; D010936:Plant Extracts / Q000494:pharmacology* / Q000627:therapeutic use; D012333:RNA, Messenger / Q000378:metabolism; D012867:Skin / Q000378:metabolism / Q000473:pathology; D018417:Th1 Cells / Q000166:cytology* / Q000276:immunology; D018418:Th2 Cells / Q000166:cytology* / Q000276:immunology
chemical_list: D016207:Cytokines; D034921:Lamin Type B; D016328:NF-kappa B; D010936:Plant Extracts; D012333:RNA, Messenger; D007073:Immunoglobulin E; D051550:I-kappa B Kinase; D020928:Mitogen-Activated Protein Kinases