title: Controlled nanoparticle release from a hydrogel by DNA-mediated particle disaggregation.
abstract: For many pharmaceutical applications, it is important that different drugs are present in the human body at distinct time points. Typically, this is achieved by a sequential administration of different therapeutic agents. A much easier alternative would be to develop a drug delivery system containing a whole set of medically active compounds which are liberated in an orchestrated and controlled manner. Yet, such a controlled, sequential release of drugs from a carrier system that can be used in a physiological situation is difficult to achieve. Here, we combine two molecular mechanisms, i.e. a build-up of osmotic pressure by the depletion of a control molecule and triggered disaggregation of nanoparticle clusters by synthetic DNA sequences. With this approach, we gain spatio-temporal control over the release of molecules and nanoparticles from a gel environment. The strategy presented here has strong implications for developing complex drug delivery systems for wound healing applications or for the sustained release of pharmaceuticals from a drug-loaded gel and will lower the need for multiple drug administrations.
authors.affiliation: Department of Mechanical Engineering and Institute of Medical Engineering, Technical University of Munich, Boltzmannstrasse 11, 85748 Garching, Germany.
authors.forename: C
authors.initials: C
authors.lastname: Nowald
authors.affiliation: Department of Mechanical Engineering and Institute of Medical Engineering, Technical University of Munich, Boltzmannstrasse 11, 85748 Garching, Germany.
authors.forename: B T
authors.initials: BT
authors.lastname: Käsdorf
authors.affiliation: Department of Mechanical Engineering and Institute of Medical Engineering, Technical University of Munich, Boltzmannstrasse 11, 85748 Garching, Germany. Electronic address: oliver.lieleg@tum.de.
authors.forename: O
authors.initials: O
authors.lastname: Lieleg
mesh_terms: D001483:Base Sequence; D004247:DNA / Q000737:chemistry*; D003692:Delayed-Action Preparations / Q000737:chemistry*; D016503:Drug Delivery Systems / Q000379:methods*; D005452:Fluoresceins / Q000008:administration & dosage; D005456:Fluorescent Dyes / Q000008:administration & dosage; D006046:Gold / Q000008:administration & dosage / Q000737:chemistry*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D009997:Osmotic Pressure
chemical_list: D003692:Delayed-Action Preparations; D005452:Fluoresceins; D005456:Fluorescent Dyes; D008081:Liposomes; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006046:Gold; D004247:DNA; C007740:fluorexon
keywords: DNA linker; Drug delivery; Liposomes; Nanoparticles; Osmotic pressure