title: Two new compounds from Atractylodes macrocephala with neuroprotective activity.
abstract: In the present study, two new compounds, together with six known compounds, were isolated from rhizome of Atractylodes macrocephala Koidz by a series of silica gel, ODS column chromatography, and preparative HPLC. Their structures were characterized as atractylenolide II (1), atractylenolide I (2), biepiasterolid (3), isoatractylenolide I (4), atractylenolide III (5), 3β-acetoxyl atractylenolide I (6), (4E,6E,12E)-tetradeca-4,6,12-triene-8,10-diyne-13,14-triol (7), (3S,4E,6E,12E)-1-acetoxy-tetradeca-4,6,12-triene-8,10-diyne-3,14-diol (8) on the basis of 1D, 2D NMR, and circular dichroism analyses. Among them, compounds 6 and 8 were novel compounds. In addition, their neuroprotective activity against MPP+-induced SH-SY5Y cells was evaluated by MTT colorimetry. The results showed that all these compounds have definite protective effect on MPP+-induced SH-SY5Y cells.
authors.affiliation: a College of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian 116600 , China.
authors.forename: Na
authors.initials: N
authors.lastname: Zhang
authors.affiliation: a College of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian 116600 , China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: b College of Pharmaceutical Engineering , Shenyang Pharmaceutical University , Shenyang 110016 , China.
authors.forename: Tie-Min
authors.initials: TM
authors.lastname: Sun
authors.affiliation: a College of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian 116600 , China.
authors.forename: Xiao-Ku
authors.initials: XK
authors.lastname: Ran
authors.affiliation: a College of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian 116600 , China.
authors.forename: Ting-Guo
authors.initials: TG
authors.lastname: Kang
authors.affiliation: a College of Pharmacy , Liaoning University of Traditional Chinese Medicine , Dalian 116600 , China.
authors.forename: De-Qiang
authors.initials: DQ
authors.lastname: Dou
mesh_terms: D031174:Atractylodes / Q000737:chemistry*; D004365:Drugs, Chinese Herbal / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D006801:Humans; D007783:Lactones / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D015394:Molecular Structure; D018696:Neuroprotective Agents / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D027343:Rhizome / Q000737:chemistry; D012717:Sesquiterpenes / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology*
chemical_list: D004365:Drugs, Chinese Herbal; D007783:Lactones; D018696:Neuroprotective Agents; D012717:Sesquiterpenes; C424804:atractylenolide I; C458582:atractylenolide II; C424802:atractylenolide III
keywords: Atractylodes macrocephala; atractylenolide; neuroprotective effect