title: Dwarf and short grain 1, encoding a putative U-box protein regulates cell division and elongation in rice.
abstract: Plant hormones coordinate a plant's responses to environmental stimuli and the endogenous developmental programs for cell division and elongation. Brassinosteroids are among the most important of these hormones in plant development. Recently, the ubiquitin-26S-proteasome system was identified to play a key role in hormone biology. In this study, we analyzed the function of a rice (Oryza sativa) gene, DSG1, which encodes a U-box E3 ubiquitin ligase. In the dsg1 mutant (an allelic mutant of tud1), the lengths of the roots, internodes, panicles, and seeds were shorter than that in the wild-type, which was due to defects in cell division and elongation. In addition, the leaves of the dsg1 mutant were wider and curled. The DSG1 protein is nuclear- and cytoplasm-localized and does not show tissue specificity in terms of its expression, which occurs in roots, culms, leaves, sheaths, and spikelets. The dsg1 mutant is less sensitive to brassinosteroid treatment than the wild-type, and DSG1 expression is negatively regulated by brassinosteroids, ethylene, auxin, and salicylic acid. These results demonstrate that DSG1 positively regulates cell division and elongation and may be involved in multiple hormone pathways.
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Nan
authors.initials: N
authors.lastname: Wang
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Yadi
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Qijin
authors.initials: Q
authors.lastname: Lou
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Feng
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Song
authors.initials: S
authors.lastname: Liu
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Meidan
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Wuzhong
authors.initials: W
authors.lastname: Yin
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Shunran
authors.initials: S
authors.lastname: Fang
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Tianquan
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China.
authors.forename: Xianchun
authors.initials: X
authors.lastname: Sang
authors.affiliation: Key Laboratory of Application and Safety Control of Genetically Modified Crops, Rice Research Institute of Southwest University, Chongqing, 400716, PR China. Electronic address: hegh@swu.edu.cn.
authors.forename: Guanghua
authors.initials: G
authors.lastname: He
mesh_terms: D060406:Brassinosteroids / Q000494:pharmacology; D002455:Cell Division; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D048430:Cell Shape / Q000187:drug effects; D002874:Chromosome Mapping; D003001:Cloning, Molecular; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D005816:Genetic Complementation Test; D009154:Mutation / Q000235:genetics; D012275:Oryza / Q000166:cytology* / Q000378:metabolism*; D010641:Phenotype; D063245:Plant Development / Q000187:drug effects; D010937:Plant Growth Regulators / Q000494:pharmacology; D018515:Plant Leaves / Q000187:drug effects / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D021381:Protein Transport / Q000187:drug effects; D013260:Steroids, Heterocyclic / Q000494:pharmacology; D013347:Subcellular Fractions / Q000378:metabolism
chemical_list: D060406:Brassinosteroids; D010937:Plant Growth Regulators; D010940:Plant Proteins; D013260:Steroids, Heterocyclic; C023623:brassinolide
keywords: Brassinosteroid; Cell division; Cell elongation; Multiple hormone pathways