title: Baicalin Inhibits Renal Cell Apoptosis and Protects Against Acute Kidney Injury in Pediatric Sepsis.
abstract: BACKGROUND Pediatric sepsis has high morbidity in children, may lead to acute kidney injury (AKI), and further aggravate the disease. Baicalin is a kind of flavonoid in Scutellaria baicalensis Georgi and has been reported to protect against several diseases, but its roles in septic AKI remain unclear. This study aimed to uncover the effects of baicalin in AKI during pediatric sepsis. MATERIAL AND METHODS Blood urea nitrogen (BUN) and serum creatinine (Cr) levels were detected in 50 pediatric patients, who underwent basic therapy with or without baicalin adjunctive therapy. Mouse sepsis models were constructed by cecal ligation and puncture (CLP) and treated with baicalin intragastrically, after which BUN and Cr examination, TUNEL apoptosis assay, and expression analyses of BAX and BCL2 were performed. RESULTS Baicalin adjunctive therapy significantly decreased BUN and Cr levels in pediatric sepsis patients (P<0.05). CLP led to elevated BUN and Cr levels in the mouse model (P<0.01), indicating kidney injury accompanied by sepsis. Baicalin decreased BUN and Cr levels (P<0.05), and reduced the apoptotic cell percent in the renal tissue (P<0.05) of the CLP model. It inhibited BAX and promoted BCL2 in the renal tissue, which was consistent with cell apoptosis changes. CONCLUSIONS Baicalin is capable of suppressing renal cell apoptosis and protecting against AKI in pediatric sepsis. This study provides a potential adjunctive therapy for treating AKI in pediatric sepsis, and further research is necessary to reveal its deeper mechanisms.
authors.affiliation: Department of Pediatrics, Liaocheng People's Hospital, Liaocheng, Shandong, China (mainland).
authors.forename: Yanping
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Pediatrics I, Binzhou City Center Hospital, Binzhou, Shandong, China (mainland).
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Fu
authors.affiliation: Department of Pharmacy, Weifang People's Hospital, Weifang, Shandong, China (mainland).
authors.forename: Hairong
authors.initials: H
authors.lastname: Lin
mesh_terms: D058186:Acute Kidney Injury / Q000188:drug therapy* / Q000503:physiopathology / Q000517:prevention & control*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D002432:Cecum / Q000473:pathology; D002648:Child; D002675:Child, Preschool; D004195:Disease Models, Animal; D005419:Flavonoids / Q000494:pharmacology* / Q000627:therapeutic use*; D006801:Humans; D007223:Infant; D007668:Kidney / Q000187:drug effects / Q000473:pathology* / Q000503:physiopathology; D007677:Kidney Function Tests; D008026:Ligation; D008297:Male; D008810:Mice, Inbred C57BL; D011677:Punctures; D018805:Sepsis / Q000188:drug therapy* / Q000517:prevention & control*; D051028:bcl-2-Associated X Protein / Q000235:genetics / Q000378:metabolism
chemical_list: D005419:Flavonoids; D051028:bcl-2-Associated X Protein; C038044:baicalin