title: [hnRNP K Contributes to Adriamycin Resistance in Acute Myeloid Leukemia through Regulating Autophagy].
abstract: OBJECTIVE
  To explore the role of heterogeneous nuclear ribonucleoprotein(hnRNP) K regulating autophagy in the drug resistance of acute myeloid leukemia, so as to provide a new molecular marker for treatment of leukemia.
  
  
  METHODS
  The relationship between the expression level of hnRNP K and the drug resistance of myeloid leukemia was verified by fluorescence quantitative PCR; the expression of autophagy related protein LC3I/ II was detected by Western blot after the hnRNP K was modulated by RNA interference technology; the sensitivity of leukemia cells to doxorubicin was analyzed before and after the expression of hnRNP K were modulatd.
  
  
  RESULTS
  The expression of hnRNP K and LC3I/II significantly increased in bone marrow nonremission patients and in drug resistant cell line, however, the expression of LC3I/ II decreased when the expression of hnRNP K were reduced, while the sensitivity of cells to adriamycin could be recovered.
  
  
  CONCLUSIONS
  hnRNP K may be involved in the formation of adriamycin resistance in acute myeloid leukemia by regulating autophagy.
authors.affiliation: Department of Paediatric Hematology and Oncology, Clinical Center of Tumor Therapy, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China. E-mail:jinfangzhang1216@126.com.
authors.forename: Jin-Fang
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Xiao-Li
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Department of Paediatric Hematology and Oncology, Clinical Center of Tumor Therapy, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
authors.forename: Yu-Deng
authors.initials: YD
authors.lastname: Lin
authors.affiliation: Department of Hematology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China.
authors.forename: Yu-Ling
authors.initials: YL
authors.lastname: Li
authors.affiliation: Department of Paediatric Hematology and Oncology, Clinical Center of Tumor Therapy, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
authors.forename: Jian-Wei
authors.initials: JW
authors.lastname: Pan
authors.affiliation: Department of Paediatric Hematology and Oncology, Clinical Center of Tumor Therapy, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
authors.forename: Sa
authors.initials: S
authors.lastname: Zong
authors.affiliation: Department of Paediatric Hematology and Oncology, Clinical Center of Tumor Therapy, Guangdong Provincial People Hospital, Guangdong Provincial Academy of Medical Sciences, Guangzhou 510080, Guangdong Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
mesh_terms: D001343:Autophagy; D045744:Cell Line, Tumor; D004317:Doxorubicin; D019008:Drug Resistance, Neoplasm; D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D034622:RNA Interference
chemical_list: D034641:Heterogeneous-Nuclear Ribonucleoprotein K; D004317:Doxorubicin