title: Low-temperature thermal reduction of suspended graphene oxide film for electrical sensing of DNA-hybridization.
abstract: A reduced graphene oxide (RGO) based capacitive real time bio-sensor was presented. Suspended graphene oxide (GO) film was assembled electrophoretically between the source and drain electrodes of a transistor and then reduced by annealing in hydrogen/nitrogen forming gas to optimize the surface functional groups and conductivity. The resonance frequency of the transmission coefficient (S21) of the transistor was observed to shift with deoxyribonucleic acid (DNA)-hybridization, with a detecting limit of ~5nM. The advantages of the bio-sensing approach include low-noise frequency output, solution based real time detection and capable of on-chip integration.
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, 361005, PR China.
authors.forename: Tun
authors.initials: T
authors.lastname: Wang
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, 361005, PR China.
authors.forename: Hong-Chen
authors.initials: HC
authors.lastname: Guo
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, 361005, PR China. Electronic address: chenxinyi@xmu.edu.cn.
authors.forename: Xin-Yi
authors.initials: XY
authors.lastname: Chen
authors.affiliation: Pen-Tung Sah Institute of Micro-Nano Science & Technology, Xiamen University, 361005, PR China. Electronic address: lm@xmu.edu.cn.
authors.forename: Miao
authors.initials: M
authors.lastname: Lu
mesh_terms: D015374:Biosensing Techniques; D003080:Cold Temperature; D004247:DNA / Q000032:analysis*; D055664:Electrochemical Techniques / Q000295:instrumentation; D004566:Electrodes; D005740:Gases / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D061925:Immobilized Nucleic Acids / Q000737:chemistry; D057230:Limit of Detection; D018625:Microscopy, Atomic Force; D009693:Nucleic Acid Hybridization; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry
chemical_list: D005740:Gases; D061925:Immobilized Nucleic Acids; D010087:Oxides; D006108:Graphite; D004247:DNA
keywords: DNA detection; Suspended graphene oxide film; Thermal reduction