title: In vivo biodistribution of antihyperglycemic biopolymer-based nanoparticles for the treatment of type 1 and type 2 diabetes.
abstract: This study aimed to assess the biodistribution of antihyperglycemic insulin-loaded alginate/dextran sulfate-based nanoparticles dual coated with chitosan and technetium-99m-albumin (99mTc-BSA) after oral administration. The oral administration of 50IU/kg insulin-loaded nanoparticles to type 1 diabetic rats showed prolonged antihyperglycemic effects up to 12h and relative pharmacological availability of 5.04% comparing to the subcutaneous administration. The oral antihyperglycemic effect was further compared between type 1 and type 2 diabetic models by the intraperitoneal glucose tolerance test, revealing that the effect lasted longer in the type 1 diabetic model.99mTc-BSA revealed to be a good nanoparticles' tracer since there was no systemic absorption and99mTc-BSA-nanoparticles were capable of increasing their residence time in the intestinal epithelium of balb-c mice when compared with99mTc-BSA biodistribution. Thus, this biopolymeric-based delivery nanoparticulate system is a promising tool for the therapy of type 1 and type 2 diabetic individuals and prevention of T1D.
authors.affiliation: Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal; CNC-Center for Neuroscience and Cell Biology, 3004-504 Coimbra, Portugal.
authors.forename: Marlene
authors.initials: M
authors.lastname: Lopes
authors.affiliation: Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal; CNC-Center for Neuroscience and Cell Biology, 3004-504 Coimbra, Portugal; Biophysics Institute, Faculty of Medicine, University of Coimbra, Portugal; CIMAGO, FMUC-Faculty of Medicine, University of Coimbra, Portugal; CNC.IBILI, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Denise
authors.initials: D
authors.lastname: Aniceto
authors.affiliation: Biophysics Institute, Faculty of Medicine, University of Coimbra, Portugal; CIMAGO, FMUC-Faculty of Medicine, University of Coimbra, Portugal; CNC.IBILI, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Margarida
authors.initials: M
authors.lastname: Abrantes
authors.affiliation: Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal; CNC-Center for Neuroscience and Cell Biology, 3004-504 Coimbra, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Simões
authors.affiliation: Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Fábio
authors.initials: F
authors.lastname: Branco
authors.affiliation: Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal; IBILI-Institute for Biomedical Imaging and Life Sciences, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Vitória
authors.affiliation: Biophysics Institute, Faculty of Medicine, University of Coimbra, Portugal; CIMAGO, FMUC-Faculty of Medicine, University of Coimbra, Portugal; CNC.IBILI, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: M Filomena
authors.initials: MF
authors.lastname: Botelho
authors.affiliation: CNC.IBILI, University of Coimbra, 3000-548 Coimbra, Portugal.
authors.forename: Raquel
authors.initials: R
authors.lastname: Seiça
authors.affiliation: Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal; CNC-Center for Neuroscience and Cell Biology, 3004-504 Coimbra, Portugal.
authors.forename: Francisco
authors.initials: F
authors.lastname: Veiga
authors.affiliation: Faculty of Pharmacy, University of Coimbra, 3000-548 Coimbra, Portugal; I3S-Instituto de Investigacão e Inovação em Saúde, University of Porto, 4150-180 Porto, Portugal; IBMC-Instituto de Biologia Molecular e Celular, 4150-180 Porto, Portugal. Electronic address: aribeiro@ff.uc.pt.
authors.forename: António
authors.initials: A
authors.lastname: Ribeiro
mesh_terms: D000818:Animals; D001704:Biopolymers; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy*; D003922:Diabetes Mellitus, Type 1 / Q000188:drug therapy; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy; D005951:Glucose Tolerance Test; D007004:Hypoglycemic Agents / Q000493:pharmacokinetics* / Q000627:therapeutic use; D007328:Insulin / Q000008:administration & dosage; D008297:Male; D008855:Microscopy, Electron, Scanning / Q000379:methods; D053758:Nanoparticles; D051381:Rats; D017208:Rats, Wistar; D013311:Streptozocin; D014018:Tissue Distribution
chemical_list: D001704:Biopolymers; D007004:Hypoglycemic Agents; D007328:Insulin; D013311:Streptozocin
keywords: Biodistribution; Insulin; Oral delivery; Type 1 diabetes; Type 2 diabetes