title: Complete genome sequence of new bacteriophage phiE142, which causes simultaneously lysis of multidrug-resistantEscherichia coliO157:H7 andSalmonella enterica.
abstract: The emergence of antibiotic-resistant foodborne bacteria is a global health problem that requires immediate attention. Bacteriophages are a promising biotechnological alternative approach against bacterial pathogens. However, a detailed analysis of phage genomes is essential to assess the safety of the phages prior to their use as biocontrol agents. Therefore, here we report the complete genome sequence of bacteriophage phiE142, which is able to lyseSalmonellaand multidrug-resistantEscherichia coliO157:H7 strains. Bacteriophage phiE142 belongs to theMyoviridaefamily due to the presence of long non-flexible tail and icosahedral head. The genome is composed of 121,442 bp and contains 194 ORFs, and 2 tRNAs. Furthermore, the phiE142 genome does not contain any genes coding for food-borne allergens, antibiotics resistance, virulence factors, or associated with lysogenic conversion. The bacteriophage phiE142 is characterized by broad host range and compelling genetic attributes making them potential candidates as a biocontrol agent.
authors.affiliation: Laboratorio de Biología Molecular y Genómica Funcional, Centro de Investigación en Alimentación y Desarrollo, A.C, Culiacán, Sinaloa Mexico ; Laboratorio de Genética, Instituto de Investigación Lightbourn. A. C, Cd. Jiménez, Chihuahua Mexico.
authors.forename: Luis
authors.initials: L
authors.lastname: Amarillas
authors.affiliation: Laboratorio Nacional para la Investigación en Inocuidad Alimentaria, Centro de Investigación en Alimentación y Desarrollo, Culiacán, Sinaloa Mexico.
authors.forename: Cristobal
authors.initials: C
authors.lastname: Chaidez
authors.affiliation: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Ciudad de México, Mexico.
authors.forename: Arturo
authors.initials: A
authors.lastname: González-Robles
authors.affiliation: Laboratorio de Biología Molecular y Genómica Funcional, Centro de Investigación en Alimentación y Desarrollo, A.C, Culiacán, Sinaloa Mexico.
authors.forename: Josefina
authors.initials: J
authors.lastname: León-Félix
keywords: Enterobacteriaceae bacteriophage; Genome sequence; Potential biocontrol agent; Short genome report; phiE142