title: Molecular Approaches in HFpEF: MicroRNAs and iPSC-Derived Cardiomyocytes.
abstract: Heart failure with preserved left ventricular ejection fraction (HFpEF) has emerged as one of the largest unmet needs in cardiovascular medicine. HFpEF is increasing in prevalence and causes significant morbidity, mortality, and health care resource utilization. Patients have multiple co-morbidities which contribute to the disease complexity. To date, no effective treatment for HFpEF has been identified. The paucity of cardiac biopsies from this patient population and the absence of well-accepted animal models limit our understanding of the underlying molecular mechanisms of HFpEF. In this review, we discuss combining state-of-the-art technologies of microRNA profiling and human induced pluripotent cell-derived cardiomyocytes (iPSC-CMs) in order to uncover novel molecular pathways that may contribute to the development of HFpEF. Here, we focus the advantages and limitations of microRNA profiling and iPSC-CMs as a disease model system to discover molecular mechanisms in HFpEF.
authors.affiliation: Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Alison J
authors.initials: AJ
authors.lastname: Kriegel
authors.affiliation: Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Melanie
authors.initials: M
authors.lastname: Gartz
authors.affiliation: Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA.
authors.forename: Muhammad Z
authors.initials: MZ
authors.lastname: Afzal
authors.affiliation: Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
authors.forename: Willem J
authors.initials: WJ
authors.lastname: de Lange
authors.affiliation: Department of Pediatrics, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA.
authors.forename: J Carter
authors.initials: JC
authors.lastname: Ralphe
authors.affiliation: Department of Medicine, Medical College of Wisconsin, Milwaukee, WI, USA. jstrande@mcw.edu.
authors.forename: Jennifer L
authors.initials: JL
authors.lastname: Strande
mesh_terms: D000818:Animals; D002460:Cell Line; D005786:Gene Expression Regulation; D006333:Heart Failure / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000378:metabolism* / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D032383:Myocytes, Cardiac / Q000378:metabolism* / Q000473:pathology; D010641:Phenotype; D015398:Signal Transduction; D013318:Stroke Volume
chemical_list: D035683:MicroRNAs
keywords: Cardiomyocytes; Engineered heart tissues; Heart failure with preserved ejection fraction; Human induced pluripotent stem cells; MicroRNA