title: Functional dichotomy and distinct nanoscale assemblies of a cell cycle-controlled bipolar zinc-finger regulator.
abstract: Protein polarization underlies differentiation in metazoans and in bacteria. How symmetric polarization can instate functional asymmetry remains elusive. Here, we show by super-resolution photo-activated localization microscopy and edgetic mutations that the bitopic zinc-finger protein ZitP implements specialized developmental functions - pilus biogenesis and multifactorial swarming motility - while shaping distinct nanoscale (bi)polar architectures in the asymmetric model bacteriumCaulobacter crescentus. Polar assemblage and accumulation of ZitP and its effector protein CpaM are orchestrated in time and space by conserved components of the cell cycle circuitry that coordinate polar morphogenesis with cell cycle progression, and also act on the master cell cycle regulator CtrA. Thus, this novel class of potentially widespread multifunctional polarity regulators is deeply embedded in the cell cycle circuitry.
authors.affiliation: Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Johann
authors.identifier: 0000-0002-3721-4307
authors.initials: J
authors.lastname: Mignolet
authors.affiliation: Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.|Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle, United Kingdom.
authors.forename: Seamus
authors.identifier: 0000-0002-7169-907X
authors.initials: S
authors.lastname: Holden
authors.affiliation: Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Bergé
authors.affiliation: Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Gaël
authors.initials: G
authors.lastname: Panis
authors.affiliation: Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Ezgi
authors.initials: E
authors.lastname: Eroglu
authors.affiliation: Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Laurence
authors.initials: L
authors.lastname: Théraulaz
authors.affiliation: Laboratory of Experimental Biophysics, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
authors.forename: Suliana
authors.initials: S
authors.lastname: Manley
authors.affiliation: Microbiology and Molecular Medicine, Institute of Genetics and Genomics in Geneva (iGE3), Faculty of Medicine, University of Geneva, Geneva, Switzerland.
authors.forename: Patrick H
authors.identifier: 0000-0002-5249-9910
authors.initials: PH
authors.lastname: Viollier
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D016935:Caulobacter crescentus / Q000235:genetics / Q000378:metabolism / Q000502:physiology*; D002453:Cell Cycle; D010861:Fimbriae, Bacterial / Q000378:metabolism; D015964:Gene Expression Regulation, Bacterial; D008124:Locomotion; D008853:Microscopy; D009154:Mutation; D001678:Organelle Biogenesis; D016335:Zinc Fingers
chemical_list: D001426:Bacterial Proteins
keywords: Caulobacter crescentus; PALM; PopZ; ZitP; cell biology; motility; pilus