title: Long Non-Coding RNA: Potential Diagnostic and Therapeutic Biomarker for Major Depressive Disorder.
abstract: BACKGROUND The criteria for diagnosing depression are based on behavioral observation and self-reporting of symptoms by the patients or guardians without any biological validation of the disease. This study aimed to identify long non-coding RNAs (lncRNAs) in peripheral blood mononuclear cells (PBMCs) as robust and predictive biomarkers for diagnosis and therapy response in major depressive disorder (MDD). MATERIAL AND METHODS We used human lncRNA 3.0 microarray profiling (which covers 30,586 human lncRNAs), using PBMCs from five MDD patients and five controls. Differentially expressed lncRNAs in the PBMCs of MDD patients were identified, of which 10 candidate lncRNAs were selected for real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis in a larger cohort of 138 MDD patients and 63 healthy controls. Then among the 138 MDD patients who received standard antidepressant treatment, 30 were randomly selected for lncRNAs expression retesting and symptomatology assessments after three-weeks and six-weeks of antidepressant treatment. RESULTS Six lncRNAs (TCONS_00019174, ENST00000566208, NONHSAG045500, ENST00000517573, NONHSAT034045, and NONHSAT142707) were significantly downregulated in MDD patients compared to control patients, and the area under the receiver operator curve (ROC) of these six lncRNAs cases, combined, was 0.719 (95% confidence interval (CI): 0.617-0.821). There was no difference in the expression of these six lncRNAs based on gender (p>0.05) or age (p>0.05). CONCLUSIONS These results suggest that the combined expression of six lncRNAs in PBMCs may serve as a potential biomarker for diagnosis and therapy response of MDD in the clinical setting.
authors.affiliation: Department of Women Health Care, Changzhou Maternity and Child Health Care Hospital Affiliated with Nanjing Medical University, Changzhou, Jiangsu, China (mainland).
authors.forename: Xuelian
authors.initials: X
authors.lastname: Cui
authors.affiliation: Department of Psychology and Psychiatry, PingAn Health Cloud Company Ltd. of China, Shanghai, China (mainland).
authors.forename: Xinyang
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Rehabilitation, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Niu
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Lingming
authors.initials: L
authors.lastname: Kong
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Mingjun
authors.initials: M
authors.lastname: He
authors.affiliation: Department of Laboratory, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Aifang
authors.initials: A
authors.lastname: Zhong
authors.affiliation: Department of Neurology, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Shengdong
authors.initials: S
authors.lastname: Chen
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Kunhong
authors.initials: K
authors.lastname: Jiang
authors.affiliation: Prevention and Treatment Center for Psychological Diseases, No. 102 Hospital of Chinese People's Liberation Army, Changzhou, Jiangsu, China (mainland).
authors.forename: Liyi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Wuxi Mental Health Center Affiliated with Nanjing Medical University, Wuxi, Jiangsu, China (mainland).
authors.forename: Zaohuo
authors.initials: Z
authors.lastname: Cheng
mesh_terms: D000328:Adult; D000928:Antidepressive Agents / Q000627:therapeutic use; D015415:Biomarkers / Q000378:metabolism; D016022:Case-Control Studies; D003865:Major Depressive Disorder / Q000175:diagnosis* / Q000188:drug therapy / Q000235:genetics / Q000628:therapy*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D063990:Gene Ontology; D006801:Humans; D008297:Male; D020411:Oligonucleotide Array Sequence Analysis; D062085:RNA, Long Noncoding / Q000235:genetics* / Q000378:metabolism; D012372:ROC Curve; D060888:Real-Time Polymerase Chain Reaction; D015203:Reproducibility of Results
chemical_list: D000928:Antidepressive Agents; D015415:Biomarkers; D062085:RNA, Long Noncoding