title: A Synthetic Hybrid Promoter for Xylose-Regulated Control of Gene Expression in Saccharomyces Yeasts.
abstract: Metabolism of non-glucose carbon sources is often highly regulated at the transcriptional and post-translational levels. This level of regulation is lacking in Saccharomyces cerevisiae strains engineered to metabolize xylose. To better control transcription in S. cerevisiae, the xylose-dependent, DNA-binding repressor (XylR) from Caulobacter crescentus was used to block transcription from synthetic promoters based on the constitutive Ashbya gossypii TEF promoter. The new hybrid promoters were repressed in the absence of xylose and showed up to a 25-fold increase in the presence of xylose. Activation of the promoter was highly sensitive to xylose with activity seen at concentrations below 2 μM xylose. These new xylose-inducible promoters allow improved control of gene expression for engineered strains of Saccharomyces yeasts.
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA. ronald.hector@ars.usda.gov.
authors.forename: Ronald E
authors.initials: RE
authors.lastname: Hector
authors.affiliation: Bioenergy Research Unit, National Center for Agricultural Utilization Research, USDA-Agricultural Research Service, 1815 N. University Street, Peoria, IL, 61604, USA.
authors.forename: Jeffrey A
authors.initials: JA
authors.lastname: Mertens
mesh_terms: D016935:Caulobacter crescentus / Q000235:genetics / Q000378:metabolism; D003001:Cloning, Molecular; D015964:Gene Expression Regulation, Bacterial; D005813:Genes, Synthetic; D011401:Promoter Regions, Genetic; D012097:Repressor Proteins / Q000235:genetics*; D012441:Saccharomyces cerevisiae / Q000235:genetics*; D058615:Synthetic Biology / Q000379:methods*; D014158:Transcription, Genetic; D014994:Xylose / Q000378:metabolism*
chemical_list: D012097:Repressor Proteins; D014994:Xylose
keywords: Caulobacter crescentus; Gene regulation; Promoter; Saccharomyces; Xylose inducible