title: Proteomic Analysis of Kidney Preservation Solutions Prior to Renal Transplantation.
abstract: One of the main issues in kidney transplantation is the optimal functional preservation of the organ until its transplantation into the appropriate recipient. Despite intensive efforts, the functional preservation period remains limited to hours. During this time, as a result of cellular injury, various proteins, peptides, and other molecules are released by the organ into the preservation medium. In this study, we used proteomic techniques to analyze the protein profiles of preservation solutions in which organs had been preserved prior to their transplantation. Samples were obtained from the preservation solutions of 25 deceased donor kidneys scheduled for transplantation. The protein profiles of the solutions were analyzed using 2D gel electrophoresis/MALDI-TOF and LC-MS/MS. We identified and quantified 206 proteins and peptides belonging to 139 different groups. Of these, 111 proteins groups were belonging to kidney tissues. This study used proteomic techniques to analyze the protein profiles of organ preservation solutions. These findings will contribute to the development of improved preservation solutions to effectively protect organs for transplantation.
authors.affiliation: Acibadem University School of Medicine, Department of Medical Biochemistry, Istanbul, Turkey.
authors.forename: Abdurrahman
authors.initials: A
authors.lastname: Coskun
authors.affiliation: Acibadem University School of Medicine, Department of Medical Biochemistry, Istanbul, Turkey.
authors.forename: Ahmet Tarik
authors.initials: AT
authors.lastname: Baykal
authors.affiliation: Marmara University Engineering Faculty, Department of Bioengineering, Istanbul, Turkey.
authors.forename: Dilek
authors.initials: D
authors.lastname: Kazan
authors.affiliation: National Metrology Institute, Gebze, Turkey.
authors.forename: Muslum
authors.initials: M
authors.lastname: Akgoz
authors.affiliation: National Metrology Institute, Gebze, Turkey.
authors.forename: Merve Oztug
authors.initials: MO
authors.lastname: Senal
authors.affiliation: Acibadem University School of Medicine Department of General Surgery, Istanbul, Turkey.
authors.forename: Ibrahim
authors.initials: I
authors.lastname: Berber
authors.affiliation: Haydarpasa Numune Research and Training Hospital, Department of General Surgery, Istanbul, Turkey.
authors.forename: Izzet
authors.initials: I
authors.lastname: Titiz
authors.affiliation: National Metrology Institute, Gebze, Turkey.
authors.forename: Gokhan
authors.initials: G
authors.lastname: Bilsel
authors.affiliation: Yeni Yuzyil University, Gaziosmanpasa Hospital, Department of Anesthesiology, Istanbul, Turkey.
authors.forename: Hakan
authors.initials: H
authors.lastname: Kilercik
authors.affiliation: Marmara University Engineering Faculty, Department of Bioengineering, Istanbul, Turkey.
authors.forename: Kubra
authors.initials: K
authors.lastname: Karaosmanoglu
authors.affiliation: Yeni Yuzyil University, Gaziosmanpasa Hospital, Department of Anesthesiology, Istanbul, Turkey.
authors.forename: Muslum
authors.initials: M
authors.lastname: Cicek
authors.affiliation: Istanbul Medipol University, Regenerative and Restorative Medicine Research Center, Beykoz/Istanbul, Turkey.
authors.forename: Ilknur
authors.initials: I
authors.lastname: Yurtsever
authors.affiliation: Erciyes University, School of Medicine, Department of Medical Biochemistry, Kayseri, Turkey.
authors.forename: Cevat
authors.initials: C
authors.lastname: Yazıcı
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D007668:Kidney / Q000378:metabolism*; D016030:Kidney Transplantation / Q000379:methods; D009926:Organ Preservation / Q000379:methods; D019188:Organ Preservation Solutions / Q000378:metabolism*; D010455:Peptides / Q000378:metabolism; D011506:Proteins / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D019188:Organ Preservation Solutions; D010455:Peptides; D011506:Proteins