title: Inhibitory effects of sodium azide on microbial growth in experimental resuspension of marine sediment.
abstract: Sodium azide (NaN3) was evaluated as inhibitor of microbial growth and activity in marine sediment resuspensions by monitoring the abundance of free-living and sessile bacteria using both flow cytometry and qPCR methods. Results show that 50mM of NaN3strongly inhibits bacterial growth under natural and enriched resource conditions.
authors.affiliation: Aix Marseille Univ, Univ Toulon, CNRS, IRD, MIO UM 110, Mediterranean Institute of Oceanography, Marseille, France. Electronic address: lea.cabrol@mio.osupytheas.fr.
authors.forename: Léa
authors.initials: L
authors.lastname: Cabrol
authors.affiliation: Aix Marseille Univ, Univ Toulon, CNRS, IRD, MIO UM 110, Mediterranean Institute of Oceanography, Marseille, France.
authors.forename: Marianne
authors.initials: M
authors.lastname: Quéméneur
authors.affiliation: PROTEE, EA 3819, Toulon University, CS 60584-83041 Toulon, Cedex 9, France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Misson
mesh_terms: D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development; D003470:Culture Media / Q000737:chemistry*; D004269:DNA, Bacterial / Q000302:isolation & purification; D004305:Dose-Response Relationship, Drug; D005434:Flow Cytometry; D019015:Geologic Sediments / Q000382:microbiology*; D010933:Plankton / Q000187:drug effects / Q000254:growth & development; D016133:Polymerase Chain Reaction; D019810:Sodium Azide / Q000633:toxicity*
chemical_list: D003470:Culture Media; D004269:DNA, Bacterial; D019810:Sodium Azide
keywords: Inhibition; Marine sediment; Microbial growth; Sodium azide (NaN(3)); Sterilization; qPCR/cytometry