title: Organophosphorous Pesticide Detection in Olive Oil by Using a Miniaturized, Easy-to-Use, and Cost-Effective Biosensor Combined with QuEChERS for Sample Clean-Up.
abstract: Herein, we report a portable electrochemical biosensor based on butyrylcholinesterase (BChE) immobilized on carbon black (CB)-modified screen-printed electrodes (SPEs) for the detection of organophosphorous pesticides in olive oil. The BChE/CB-SPE biosensor was developed to detect paraoxon in standard solutions as well as in olive oil samples previously treated with the QuEChERS method to extract pesticides from the whole fatty matrix. The biosensor shows a linear concentration range of between 20 and 100 ppb for paraoxon both in standard solutions (phosphate buffer 0.05 M) and in olive oil extracts, with a detection limit of 6 ppb in olive oil extract, corresponding to 10% of inhibition. The accuracy of this biosensor in olive oil samples was assessed with olive oil spiked with paraoxon, obtaining satisfactory recovery values.
authors.affiliation: Department of Chemical Science and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133 Rome, Italy. fabiana.arduini@uniroma2.it.
authors.forename: Fabiana
authors.initials: F
authors.lastname: Arduini
authors.affiliation: Department of Chemical Science and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133 Rome, Italy. matteo.forchielli@gmail.com.
authors.forename: Matteo
authors.initials: M
authors.lastname: Forchielli
authors.affiliation: Institute of Crystallography (IC-CNR), Via Salaria km 29.300, 00015 Monterotondo, Italy. viviana.scognamiglio@mlib.ic.cnr.it.
authors.forename: Viviana
authors.initials: V
authors.lastname: Scognamiglio
authors.affiliation: Department of Analytical Chemistry, Institute of Chemical Engineering, Ural Federal University Named After the First President of Russia B. N. Yeltsin, Mira 19, 620002 Yekaterinburg, Russian Federation. a.n.kozitsina@urfu.ru.
authors.forename: Kozitsina Alisa
authors.initials: KA
authors.lastname: Nikolaevna
authors.affiliation: Department of Chemical Science and Technologies, University of Rome "Tor Vergata", Via della Ricerca Scientifica, 00133 Rome, Italy. moscone@uniroma2.it.
authors.forename: Danila
authors.initials: D
authors.lastname: Moscone
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004563:Electrochemistry; D057230:Limit of Detection; D000069463:Olive Oil / Q000032:analysis / Q000737:chemistry*; D009943:Organophosphorus Compounds / Q000032:analysis; D010575:Pesticides / Q000032:analysis*
chemical_list: D000069463:Olive Oil; D009943:Organophosphorus Compounds; D010575:Pesticides
keywords: QuEChERS; butyrylcholinesterase; carbon black; electrochemical biosensor; organophosphorous pesticides; screen-printed electrodes