title: New Monoclonal Antibodies to Defined Cell Surface Proteins on Human Pluripotent Stem Cells.
abstract: The study and application of human pluripotent stem cells (hPSCs) will be enhanced by the availability of well-characterized monoclonal antibodies (mAbs) detecting cell-surface epitopes. Here, we report generation of seven new mAbs that detect cell surface proteins present on live and fixed human ES cells (hESCs) and human iPS cells (hiPSCs), confirming our previous prediction that these proteins were present on the cell surface of hPSCs. The mAbs all show a high correlation with POU5F1 (OCT4) expression and other hPSC surface markers (TRA-160 and SSEA-4) in hPSC cultures and detect rare OCT4 positive cells in differentiated cell cultures. These mAbs are immunoreactive to cell surface protein epitopes on both primed and naive state hPSCs, providing useful research tools to investigate the cellular mechanisms underlying human pluripotency and states of cellular reprogramming. In addition, we report that subsets of the seven new mAbs are also immunoreactive to human bone marrow-derived mesenchymal stem cells (MSCs), normal human breast subsets and both normal and tumorigenic colorectal cell populations. The mAbs reported here should accelerate the investigation of the nature of pluripotency, and enable development of robust cell separation and tracing technologies to enrich or deplete for hPSCs and other human stem and somatic cell types. Stem Cells 2017;35:626-640.
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.|Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
authors.forename: Carmel M
authors.initials: CM
authors.lastname: O'Brien
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.|Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
authors.forename: Hun S
authors.initials: HS
authors.lastname: Chy
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.|Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
authors.forename: Qi
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.
authors.forename: Shiri
authors.initials: S
authors.lastname: Blumenfeld
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.|Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
authors.forename: Jack W
authors.initials: JW
authors.lastname: Lambshead
authors.affiliation: Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.|Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Xiaodong
authors.initials: X
authors.lastname: Liu
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.|Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Joshua
authors.initials: J
authors.lastname: Kie
authors.affiliation: The Walter and Eliza Hall Institute (WEHI), Parkville, Victoria, Australia.|Department of Medical Biology.
authors.forename: Bianca D
authors.initials: BD
authors.lastname: Capaldo
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.|Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
authors.forename: Tung-Liang
authors.initials: TL
authors.lastname: Chung
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.
authors.forename: Timothy E
authors.initials: TE
authors.lastname: Adams
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.
authors.forename: Tram
authors.initials: T
authors.lastname: Phan
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.
authors.forename: John D
authors.initials: JD
authors.lastname: Bentley
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.
authors.forename: William J
authors.initials: WJ
authors.lastname: McKinstry
authors.affiliation: Department of Surgery, Cabrini Monash University, Malvern, Victoria, Australia.
authors.forename: Karen
authors.initials: K
authors.lastname: Oliva
authors.affiliation: Department of Surgery, Cabrini Monash University, Malvern, Victoria, Australia.
authors.forename: Paul J
authors.initials: PJ
authors.lastname: McMurrick
authors.affiliation: Department of Chemical Physiology.|Center for Regenerative Medicine, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Yu-Chieh
authors.initials: YC
authors.lastname: Wang
authors.affiliation: Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.|Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Fernando J
authors.initials: FJ
authors.lastname: Rossello
authors.affiliation: The Walter and Eliza Hall Institute (WEHI), Parkville, Victoria, Australia.|Department of Medicine, The University of Melbourne, Parkville, Victoria, Australia.|Department of Medical Oncology, The Royal Melbourne Hospital, Parkville, Victoria, Australia.
authors.forename: Geoffrey J
authors.initials: GJ
authors.lastname: Lindeman
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California, USA.
authors.forename: Di
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.|Cancer Program, Monash Biomedicine Discovery Institute.|Centre for Cancer Research, Hudson Institute of Medical Research, Clayton, Victoria, Australia.
authors.forename: Thierry
authors.initials: T
authors.lastname: Jarde
authors.affiliation: Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California, USA.
authors.forename: Amander T
authors.initials: AT
authors.lastname: Clark
authors.affiliation: Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.|Cancer Program, Monash Biomedicine Discovery Institute.
authors.forename: Helen E
authors.initials: HE
authors.lastname: Abud
authors.affiliation: The Walter and Eliza Hall Institute (WEHI), Parkville, Victoria, Australia.|Department of Medical Biology.
authors.forename: Jane E
authors.initials: JE
authors.lastname: Visvader
authors.affiliation: Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.|Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Nefzger
authors.affiliation: Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.|Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.
authors.forename: Jose M
authors.initials: JM
authors.lastname: Polo
authors.affiliation: Department of Chemical Physiology.|Center for Regenerative Medicine, The Scripps Research Institute, La Jolla, California, USA.
authors.forename: Jeanne F
authors.initials: JF
authors.lastname: Loring
authors.affiliation: Clayton and Parkville, CSIRO Manufacturing, Victoria, Australia.|Australian Regenerative Medicine Institute, Monash University, Clayton, Victoria, Australia.
authors.forename: Andrew L
authors.initials: AL
authors.lastname: Laslett
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000276:immunology*; D000954:Antigens, Surface / Q000378:metabolism; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D000066673:Cell Self Renewal; D015536:Down-Regulation / Q000235:genetics; D058732:Embryoid Bodies / Q000166:cytology / Q000378:metabolism; D005434:Flow Cytometry; D006412:Hematopoietic Stem Cells / Q000378:metabolism; D006801:Humans; D008565:Membrane Proteins / Q000276:immunology* / Q000378:metabolism; D051379:Mice; D050814:Octamer Transcription Factor-3 / Q000378:metabolism; D039904:Pluripotent Stem Cells / Q000378:metabolism*
chemical_list: D000911:Antibodies, Monoclonal; D000954:Antigens, Surface; D008565:Membrane Proteins; D050814:Octamer Transcription Factor-3
keywords: Breast; Cancer; Cell surface markers; Colorectal; Human embryonic stem cells; Human iPS cells; Monoclonal antibodies; Naive; Pluripotency