title: A polycystin-type transient receptor potential (Trp) channel that is activated by ATP.
abstract: ATP and ADP are ancient extra-cellular signalling molecules that inDictyosteliumamoebae cause rapid, transient increases in cytosolic calcium due to an influx through the plasma membrane. This response is independent of hetero-trimeric G-proteins, the putative IP3 receptor IplA and all P2X channels. We show, unexpectedly, that it is abolished in mutants of the polycystin-type transient receptor potential channel, TrpP. Responses to the chemoattractants cyclic-AMP and folic acid are unaffected in TrpP mutants. We report that the DIF morphogens, cyclic-di-GMP, GABA, glutamate and adenosine all induce strong cytoplasmic calcium responses, likewise independently of TrpP. Thus, TrpP is dedicated to purinergic signalling. ATP treatment causes cell blebbing within seconds but this does not require TrpP, implicating a separate purinergic receptor. We could detect no effect of ATP on chemotaxis and TrpP mutants grow, chemotax and develop almost normally in standard conditions. No gating ligand is known for the human homologue of TrpP, polycystin-2, which causes polycystic kidney disease. Our results now show that TrpP mediates purinergic signalling inDictyosteliumand is directly or indirectly gated by ATP.
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB1 0QH, UK dt101@mrc-lmb.cam.ac.uk rrk@mrc-lmb.cam.ac.uk.
authors.forename: David
authors.initials: D
authors.lastname: Traynor
authors.affiliation: MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB1 0QH, UK dt101@mrc-lmb.cam.ac.uk rrk@mrc-lmb.cam.ac.uk.
authors.forename: Robert R
authors.identifier: http://orcid.org/0000-0001-9836-7967
authors.initials: RR
authors.lastname: Kay
keywords: ATP; DIF; Dictyostelium; Polycystin-2; Purinergic signalling; Trp channel