title: Bioactive isopimarane diterpenoids from the stems of Euonymus oblongifolius.
abstract: Seven isopimarane diterpenes and one abietane diterpene, together with six known sesquiterpene derivatives, were isolated from the stems of Euonymus oblongifolius. Their structures were elucidated on the basis of spectroscopic analyses, and the absolute configuration of euonymusisopimaric acid A was confirmed by single-crystal X-ray crystallographic analysis using anomalous scattering of Cu Kα radiation. All of the isolated compounds were evaluated for their ability to inhibit LPS-induced nitric oxide production in the murine microglia BV2 cell line, and for their cytotoxic activity against five human cancer cell lines. Euonymusisopimaric acids A, E and F inhibited LPS-induced nitric oxide production in the murine microglia BV2 cell line, with IC50values of 2.4, 4.8, and 1.6 μM, respectively. Euonymusisopimaric acid B exhibited moderate cytotoxicity against A549 cell line growth, with an IC50value of 2.6 μM.
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
authors.forename: Chuang-Jun
authors.initials: CJ
authors.lastname: Li
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
authors.forename: Jing-Zhi
authors.initials: JZ
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
authors.forename: Dan
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China.
authors.forename: Xiao-Guang
authors.initials: XG
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People's Republic of China. Electronic address: zhangdm@imm.ac.cn.
authors.forename: Dong-Ming
authors.initials: DM
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D018360:Crystallography, X-Ray; D004224:Diterpenes / Q000737:chemistry* / Q000302:isolation & purification*; D029756:Euonymus / Q000737:chemistry*; D006801:Humans; D020128:Inhibitory Concentration 50
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes
keywords: Celastraceae; Cytotoxicity; Euonymus oblongifolius; Isopimarane diterpenoid; Nitric oxide production