title: [Effect of BRD4 Inhibitor GSK525762A on Proliferation and Apoptosis of SUP-B15 Cells In Vitro and Its Possible Mechanism].
abstract: OBJECTIVE
  To investigate the effect of BRD4 inhibitor GSK525762A on the proliferation and apoptosis of Philadelphia chromosome-positive acute lymphoblastic leukemia SUP-B15 cells and its mechanism.
  
  
  METHODS
  SUP-B15 cells were treated with different concentration of GSK525762A, the proliferation-inhibition curve was assayed and plotted by CCK-8 method, the cell viability and apoptosis were detected by flow cytometry with Annexin V and 7-AAD staining. The transcripts of anti-apoptotic genes C-MYC, CDK6 and BCL-2 were detected by real-time PCR.
  
  
  RESULTS
  GSK525762A could inhibit significantly SUP-B15 cell proliteration in dose-and time-dependent manner; GSK525762A treatment could induce apoptosis of SUP-B15 cells. The levels of C-MYC,CDK6 and BCL-2 mRNA transcripts in SUP-B15 cells were reduced in GSK525762A-treated group.
  
  
  CONCLUSIONS
  The GSK525762A can remarkably inhibit proliferation and induce apoptosis of SUP-B15 cells. The down-regulation of apoptosis-related genes C-MYC,CDK6 and BCL-2 may be involved in the process of apoptosis.
authors.affiliation: Institute of Hematology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Wang
authors.affiliation: Institute of Hematology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Na
authors.initials: N
authors.lastname: Qi
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002, Jiangsu Province, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002, Jiangsu Province, China.
authors.forename: Zhi-Ling
authors.initials: ZL
authors.lastname: Yan
authors.affiliation: Institute of Hematology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Qing-Yun
authors.initials: QY
authors.lastname: Wu
authors.affiliation: Institute of Hematology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Institute of Hematology, Xuzhou Medical College, Xuzhou 221002, Jiangsu Province, China. E-mail:chenchong@xzmc.edu.cn.
authors.forename: Chong
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Hematology, The Affiliated Hospital of Xuzhou Medical College,Xuzhou 221002, Jiangsu Province, China. E-mail: lihmd@163.com.
authors.forename: Kai-Lin
authors.initials: KL
authors.lastname: Xu
mesh_terms: D017209:Apoptosis; D001569:Benzodiazepines; D002452:Cell Count; D018797:Cell Cycle Proteins; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D051361:Cyclin-Dependent Kinase 6; D015536:Down-Regulation; D005434:Flow Cytometry; D006801:Humans; D009687:Nuclear Proteins; D014157:Transcription Factors; D000092468:Bromodomain Containing Proteins
chemical_list: D001569:Benzodiazepines; D018797:Cell Cycle Proteins; D051361:Cyclin-Dependent Kinase 6; D009687:Nuclear Proteins; D014157:Transcription Factors; C441289:BRD4 protein, human; D000092468:Bromodomain Containing Proteins; C554645:molibresib; C495911:CDK6 protein, human