title: Impact and Influence of the NaturalVibrio-Squid Symbiosis in Understanding Bacterial-Animal Interactions.
abstract: Animals are colonized by bacteria, and in many cases partners have co-evolved to perform mutually beneficial functions. An exciting and ongoing legacy of the past decade has been an expansion of technology to enable study of natural associationsin situ/in vivo. As a result, more symbioses are being examined, and additional details are being revealed for well-studied systems with a focus on the interactions between partners in the native context. With this framing, we review recent literature from theVibrio fischeri-Euprymna scolopessymbiosis and focus on key studies that have had an impact on understanding bacteria-animal interactions broadly. This is not intended to be a comprehensive review of the system, but rather to focus on particular studies that have excelled at moving from pattern to process in facilitating an understanding of the molecular basis to intriguing observations in the field of host-microbe interactions. In this review we discuss the following topics: processes regulating strain and species specificity; bacterial signaling to host morphogenesis; multiple roles for nitric oxide; flagellar motility and chemotaxis; and efforts to understand unannotated and poorly annotated genes. Overall these studies demonstrate how functional approachesin vivoin a tractable system have provided valuable insight into general principles of microbe-host interactions.
authors.affiliation: Department of Microbiology-Immunology, Northwestern University Feinberg School of Medicine Chicago, IL, USA.
authors.forename: Mark J
authors.initials: MJ
authors.lastname: Mandel
authors.affiliation: Department of Microbiology and Plant Biology, University of Oklahoma Norman, OK, USA.
authors.forename: Anne K
authors.initials: AK
authors.lastname: Dunn
keywords: epithelial colonization; evolution; invertebrate model; marine microbiology; microbiome; symbiosis