title: The Joule-Thomson coefficient as a criterion for efficient operating conditions in supercritical fluid chromatography.
abstract: When an SFC column is operated in a traditional oven with forced air at low pressures near the critical temperature, severe efficiency losses can occur. The mobile phase cools as it expands along the column, forming axial and radial temperature gradients. In this study we present a simple model based on a virtual fluid to predict the conditions which lead to the onset of efficiency loss. The model shows that the Joule-Thomson coefficient is an important factor leading to efficiency loss in packed columns under forced air conditions. The model was tested experimentally for elution of n-alkylbenzenes on 250×4.6-mm ID columns packed with 5-μm Luna-C18 (fully porous) and Kinetex-C18 (superficially porous) particles at optimum flow rates in a forced air oven at 20-80°C and outlet pressures from 90 to 250bar, with CO2mobile phase containing 5, 10 and 20% methanol (v/v). For simplicity, we used a formal J-T coefficient corresponding to the inlet temperature and the outlet pressure to characterize the chromatographic conditions. For 5% methanol, there was no significant loss of efficiency for elution of n-octadecylbenzene as long as the formal J-T coefficient was less than 0.11K/bar for Luna or 0.15K/bar for Kinetex, with minimum reduced plate heights equal to 1.82 and 1.55, respectively, at an average apparent retention factor of approximately 4.0 for both columns. The Kinetex column provided superior efficiency in general, and at 10-20bar lower outlet pressures relative to the Luna column due to the higher thermal conductivity of the packing. Results for 10 and 20% methanol showed similar trends but were less predictable.
authors.affiliation: Department of Chemistry and Biochemistry, University of Minnesota Duluth, Duluth, MN, 55812, USA. Electronic address: dpoe@d.umn.edu.
authors.forename: Donald P
authors.initials: DP
authors.lastname: Poe
authors.affiliation: Department of Chemistry and Biochemistry, University of Minnesota Duluth, Duluth, MN, 55812, USA.
authors.forename: Shawn
authors.initials: S
authors.lastname: Helmueller
authors.affiliation: Department of Chemistry and Biochemistry, University of Minnesota Duluth, Duluth, MN, 55812, USA.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Kobany
authors.affiliation: Department of Chemistry and Biochemistry, University of Minnesota Duluth, Duluth, MN, 55812, USA.
authors.forename: Hannah
authors.initials: H
authors.lastname: Feldhacker
authors.affiliation: Department of Chemical and Process Engineering, Rzeszow University of Technology, 35-959, Rzeszow, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Kaczmarski
mesh_terms: D000388:Air; D025924:Chromatography, Supercritical Fluid / Q000379:methods*; D007700:Kinetics; D000432:Methanol / Q000737:chemistry; D008956:Models, Chemical; D016062:Porosity; D011312:Pressure; D013696:Temperature
chemical_list: D000432:Methanol
keywords: Efficiency maps; Joule-Thomson coefficient; Kinetic performance; Radial temperature gradients; Supercritical fluid chromatography; Virtual fluid model