title: Genotypic-dependent effects of N fertilizer, glutathione, silicon, zinc, and selenium on proteomic profiles, amino acid contents, and quality of rice genotypes with contrasting grain Cd accumulation.
abstract: Soil heavy metal (HM) contamination has posed a serious problem for safe food production. For restricting the translocation of HM into grain, many proteins were regulated to involve in the process. To identify these proteins, 2D-based proteomic analysis was carried out using different rice genotypes with distinct Cd accumulation in grains and as affected by an alleviating regulator (AR) in field experiments. AR application improved grain quality, with increased contents in Glu, Cys, His, Pro, and protein. Twenty-six low-grain HM accumulation-associated protein species were identified and categorized as physiological functions via two-dimensional gel electrophoresis (2DE) and mass spectrometry. Among these proteins, 8, 9, and 9 proteins exhibited higher accumulation, lower accumulation, and unchanged accumulation, respectively, in Xiushui817 (low accumulator) vs R8097 (high accumulator) under control conditions but showed differential accumulation patterns after AR application. These proteins included sucrose synthase 3, alanine aminotransferase, glutelin, cupin family protein, and zinc finger CCCH domain-containing protein 32. The differential expression of these protein species might contribute to decreased HM accumulation in grain via decreasing the protein accumulation which had high affinity to HM or regulating energy metabolism and signal transduction. Our findings provide valuable insights into the mechanisms of low-grain HM accumulation in rice and possible utilization of candidate protein species in developing low-grain HM accumulation genotypes.
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Fangbin
authors.initials: F
authors.lastname: Cao
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Manman
authors.initials: M
authors.lastname: Fu
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Runfeng
authors.initials: R
authors.lastname: Wang
authors.affiliation: Jiaxing Academy of Agricultural Sciences, Jiaxing, 314016, People's Republic of China.
authors.forename: Wangda
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Department of Agronomy, College of Agriculture and Biotechnology, Zijingang Campus, Zhejiang University, Hangzhou, 310058, People's Republic of China. wufeibo@zju.edu.cn.
authors.forename: Feibo
authors.initials: F
authors.lastname: Wu
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D002104:Cadmium / Q000633:toxicity*; D002523:Edible Grain / Q000187:drug effects* / Q000378:metabolism; D005308:Fertilizers / Q000009:adverse effects*; D005838:Genotype; D005978:Glutathione / Q000378:metabolism; D012275:Oryza / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D020543:Proteome / Q000187:drug effects* / Q000235:genetics / Q000378:metabolism; D012643:Selenium / Q000378:metabolism; D012825:Silicon / Q000378:metabolism; D012989:Soil Pollutants / Q000633:toxicity*; D015032:Zinc / Q000378:metabolism
chemical_list: D000596:Amino Acids; D005308:Fertilizers; D020543:Proteome; D012989:Soil Pollutants; D002104:Cadmium; D005978:Glutathione; D012643:Selenium; D015032:Zinc; D012825:Silicon
keywords: Alleviation practice; Grain quality; Heavy metal; Proteomic analysis; Rice