title: Wide-Scope Screening Method for Multiclass Veterinary Drug Residues in Fish, Shrimp, and Eel Using Liquid Chromatography-Quadrupole High-Resolution Mass Spectrometry.
abstract: A screening method for veterinary drug residues in fish, shrimp, and eel using LC with a high-resolution MS instrument has been developed and validated. The method was optimized for over 70 test compounds representing a variety of veterinary drug classes. Tissues were extracted by vortex mixing with acetonitrile acidified with 2% acetic acid and 0.2% p-toluenesulfonic acid. A centrifuged portion of the extract was passed through a novel solid phase extraction cartridge designed to remove interfering matrix components from tissue extracts. The eluent was then evaporated and reconstituted for analysis. Data were collected with a quadrupole-Orbitrap high-resolution mass spectrometer using both nontargeted and targeted acquisition methods. Residues were detected on the basis of the exact mass of the precursor and a product ion along with isotope pattern and retention time matching. Semiquantitative data analysis compared MS1signal to a one-point extracted matrix standard at a target testing level. The test compounds were detected and identified in salmon, tilapia, catfish, shrimp, and eel extracts fortified at the target testing levels. Fish dosed with selected analytes and aquaculture samples previously found to contain residues were also analyzed. The screening method can be expanded to monitor for an additional >260 veterinary drugs on the basis of exact mass measurements and retention times.
authors.affiliation: Animal Drugs Research Center, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.
authors.forename: Sherri B
authors.identifier: http://orcid.org/0000-0002-0556-7781
authors.initials: SB
authors.lastname: Turnipseed
authors.affiliation: Animal Drugs Research Center, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.
authors.forename: Joseph M
authors.initials: JM
authors.lastname: Storey
authors.affiliation: Animal Drugs Research Center, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.
authors.forename: Jack J
authors.initials: JJ
authors.lastname: Lohne
authors.affiliation: Animal Drugs Research Center, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.
authors.forename: Wendy C
authors.initials: WC
authors.lastname: Andersen
authors.affiliation: Denver Laboratory, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.
authors.forename: Robert
authors.initials: R
authors.lastname: Burger
authors.affiliation: Denver Laboratory, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.
authors.forename: Aaron S
authors.initials: AS
authors.lastname: Johnson
authors.affiliation: Animal Drugs Research Center, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.|Denver Laboratory, U.S. Food and Drug Administration , Denver, Colorado 80225, United States.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Madson
mesh_terms: D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003445:Crustacea / Q000737:chemistry; D004350:Drug Residues / Q000737:chemistry* / Q000302:isolation & purification; D004524:Eels; D005399:Fishes; D005506:Food Contamination / Q000032:analysis; D017747:Seafood / Q000032:analysis*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D019155:Veterinary Drugs / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D019155:Veterinary Drugs
keywords: aquaculture; high-resolution mass spectrometry; screening methods; veterinary drug residues