title: Conversion of oat (Avena sativaL.) haploid embryos into plants in relation to embryo developmental stage and regeneration media.
abstract: Obtaining oat DH lines is only effective via wide crossing with maize. Seven hundred haploid embryos from 21 single F1progeny obtained from wide crosses with maize were isolated, divided into four groups according to their size (<0.5 mm, 0.5-0.9 mm, 1.0-1.4 mm, and ≥1.5 mm), and transferred into 190-2 regeneration medium with different growth regulators: 0.5 mg L-1kinetin (KIN) and 0.5 mg L-11-naphthaleneacetic acid (NAA); 1 mg L-1zeatin (ZEA) and 0.5 mg L-1NAA; or 1 mg L-1dicamba (DIC), 1 mg L-1picloram (PIC), and 0.5 mg L-1kinetin (KIN). Among all isolated embryos, approximately 46.1% were between 1.0-1.4 mm, while the smallest group of embryos (7.1%) were those <0.5 mm. The ability of haploid embryos to germinate varied depending on oat genotypes and the size of embryos. Haploid embryos <0.5 mm were globular and did not germinate, whereas embryos ≥1.5 mm had clearly visible coleoptiles, radicles, and scutella, and were able to germinate. Germination of oat haploid embryos varied depending on growth regulators in the regeneration medium. Most haploid embryos germinated on medium with 0.5 mg L-1NAA and 0.5 mg L-1KIN, while the fewest germinated on medium with 1 mg L-1DIC, 1 mg L-1PIC, and 0.5 mg L-1KIN. One hundred thirty germinated haploid embryos converted into haploid plants. Fifty oat DH lines were obtained after colchicine treatment.
authors.affiliation: Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
authors.forename: Angelika
authors.initials: A
authors.lastname: Noga
authors.affiliation: Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Skrzypek
authors.affiliation: Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
authors.forename: Marzena
authors.initials: M
authors.lastname: Warchoł
authors.affiliation: Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
authors.forename: Ilona
authors.initials: I
authors.lastname: Czyczyło-Mysza
authors.affiliation: Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
authors.forename: Kinga
authors.initials: K
authors.lastname: Dziurka
authors.affiliation: Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
authors.forename: Izabela
authors.initials: I
authors.lastname: Marcińska
authors.affiliation: Department of Biotechnology, Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
authors.forename: Katarzyna
authors.initials: K
authors.lastname: Juzoń
authors.affiliation: Department of Plant Breeding and Seed Science, University of Agriculture, Łobzowska 24, 31-140 Kraków, Poland.
authors.forename: Tomasz
authors.initials: T
authors.lastname: Warzecha
authors.affiliation: Department of Plant Breeding and Seed Science, University of Agriculture, Łobzowska 24, 31-140 Kraków, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Sutkowska
authors.affiliation: Plant Breeding Strzelce Ltd., PBAI Group, Główna 20, 99-307 Strzelce, Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Nita
authors.affiliation: Plant Breeding Strzelce Ltd., PBAI Group, Główna 20, 99-307 Strzelce, Poland.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Werwińska
keywords: Avena sativa L.; DH lines; Growth regulators; Haploid embryos