title: (+)-Catechin prevents methylglyoxal-induced mitochondrial dysfunction and apoptosis in EA.hy926 cells.
abstract: OBJECTIVE
  To investigate whether (+)-catechin, a strong antioxidant, can prevent methylglyoxal (MGO)-induced cytotoxicity and its mechanism.
  
  
  METHODS
  Cytotoxicity, apoptosis, reactive oxygen species (ROS) generation, hydrogen peroxide (H2O2) formation, mitochondrial membrane potential (MMP) and mitochondrial morphology were measured in EA.hy926 cells.
  
  
  RESULTS
  MGO (4 mM)-induced cytotoxicity was markedly inhibited by (+)-catechin (0.1-4 mM) in 24 h. 1 mM MGO-induced apoptotic cell death (44.7%) was significantly inhibited by 4 mM (+)-catechin (to 24.4%), 1 mM aminoguanidine (AG) (to 28.8%) or 4 mM N-acetylcysteine (NAC) (to 24.3%). (+)-Catechin (4 mM) or AG (4 mM) can inhibit the decrease of MMP induced by MGO (2-8 mM) in 3 h. (+)-Catechin (4 mM) or AG (4 mM) can inhibit MGO (4 mM)-induced mitochondrial swelling in 3 h. However, MGO (4 mM)-induced ROS and H2O2generation was not prevented by (+)-catechin (4 mM).
  
  
  CONCLUSIONS
  (+)-Catechin prevents MGO-induced cytotoxicity in EA.Hy926 cells through inhibiting apoptosis and mitochondrial damage.
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhang
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Mu
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Mingqi
authors.initials: M
authors.lastname: Yang
authors.affiliation: b CHU Sainte-Justine Research Centre , Montreal , Quebec , Canada.
authors.forename: Abdullah
authors.initials: A
authors.lastname: Al Maruf
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Panpan
authors.initials: P
authors.lastname: Li
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Chao
authors.initials: C
authors.lastname: Li
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Shaohua
authors.initials: S
authors.lastname: Dai
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Jiangyi
authors.initials: J
authors.lastname: Lu
authors.affiliation: a College of Veterinary Medicine, Northwest A&F University , Yangling , Shaanxi , China and.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Dong
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D002392:Catechin / Q000494:pharmacology*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D006801:Humans; D006861:Hydrogen Peroxide / Q000378:metabolism; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology*; D016877:Oxidants / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects; D011765:Pyruvaldehyde / Q000009:adverse effects*; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D016877:Oxidants; D017382:Reactive Oxygen Species; D011765:Pyruvaldehyde; D002392:Catechin; D006861:Hydrogen Peroxide
keywords: (+)-catechin; EA.hy926 cells; Methylglyoxal; apoptosis; mitochondrial dysfunction