title: Halomonas alkalicola sp. nov., isolated from a household product plant.
abstract: A Gram-stain-negative, alkaliphilic and moderately halophilic bacterium, designated 56-L4-10aEnT, was isolated from a household product plant in China. Cells of the novel isolate were rod-shaped, non-spore-forming and non-motile. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 56-L4-10aEnT belongs to the genus Halomonas, with the six closest neighbours being Halomonas mongoliensis Z-7009T (97.59 % 16S rRNA gene sequence similarity), Halomonas ventosae Al12T (97.35 %), Halomonas campaniensis 5AGT (97.22 %), Halomonas alimentaria YKJ-16T (97.22 %), Halomonas shengliensis SL014B-85T (97.12 %) and Halomonas fontilapidosi 5CRT (97.09 %). The main polar lipids of strain 56-L4-10aEnT contained diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The predominant respiratory quinone was Q-9, with Q-8 as a minor component. The major fatty acids were C18 : 1ω7c/C18 : 1ω6c and C16 : 0. Strain 56-L4-10aEnT was clearly distinguished from the type strains mentioned above through phylogenetic analysis, DNA-DNA hybridization, fatty acid composition data and a range of physiological and biochemical characteristics comparisons. It is evident from the genotypic and phenotypic data that strain 56-L4-10aEnTcould be classified as a representative of a novel species of the genus Halomonas, for which the name Halomonas alkalicola sp. nov. is proposed. The type strain is 56-L4-10aEnT (=CICC 11012sT=DSM 103354T).
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Tang
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhai
authors.affiliation: Procter & Gamble Technologies (Beijing) Ltd, Beijing 101312, PR China.
authors.forename: Yafang
authors.initials: Y
authors.lastname: Lin
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Su
authors.initials: S
authors.lastname: Yao
authors.affiliation: Procter & Gamble Technologies (Beijing) Ltd, Beijing 101312, PR China.
authors.forename: Lijiang
authors.initials: L
authors.lastname: Wang
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ge
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhang
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Tianci
authors.initials: T
authors.lastname: Zhang
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Procter & Gamble International Operations SA Singapore Branch, 70 Biopolis Street, 138547, Singapore.
authors.forename: Jiquan
authors.initials: J
authors.lastname: Liu
authors.affiliation: China Center of Industrial Culture Collection (CICC), China National Research, Institute of Food and Fermentation Industries, Beijing 100015, PR China.
authors.forename: Chi
authors.initials: C
authors.lastname: Cheng
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D004275:DNA, Ribosomal / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D005838:Genotype; D020632:Halomonas / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D006795:Household Products; D009693:Nucleic Acid Hybridization; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9