title: Neonatal capsaicin administration impairs postnatal development of the cardiac chronotropy and inotropy in rats.
abstract: The present study evaluated the impact of neonatal administration of capsaicin (neurotoxin from red hot pepper used for sensory denervation) on postnatal development of the heart rate and ventricular contractility. In the rats subjected to capsaicin administration (100 mg/kg) on postnatal days 2 and 3 and their vehicle-treated controls at the ages of 10 to 90 days, function of the sympathetic innervation of the developing heart was characterized by evaluation of chronotropic responses to metipranolol and atropine, norepinephrine concentrations in the heart, and norepinephrine release from the heart atria. Sensory denervation was verified by determination of calcitonin gene-related peptide levels in the heart. Direct cytotoxic effects of capsaicin were assessed on cultured neonatal cardiomyocytes. Capsaicin-treated rats displayed higher resting heart rates, lower atropine effect, but no difference in the effect of metipranolol. Norepinephrine tissue levels and release did not differ from controls. Contraction force of the right ventricular papillary muscle was lower till the age of 60 days. Significantly reduced viability of neonatal cardiomyocytes was demonstrated at capsaicin concentration 100 micromol/l. Our study suggests that neonatal capsaicin treatment leads to impaired maturation of the developing cardiomyocytes. This effect cannot be attributed exclusively to sensory denervation of the rat heart since capsaicin acts also directly on the cardiac cells.
authors.affiliation: Department of Physiology, Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Pilsen, Czech Republic. jitka.kuncova@lfp.cuni.cz.
authors.forename: J
authors.initials: J
authors.lastname: Kuncová
authors.forename: A
authors.initials: A
authors.lastname: Jirkovská
authors.forename: J
authors.initials: J
authors.lastname: Švíglerová
authors.forename: M
authors.initials: M
authors.lastname: Marková
authors.forename: D
authors.initials: D
authors.lastname: Meireles
authors.forename: M
authors.initials: M
authors.lastname: Čedíková
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002211:Capsaicin / Q000633:toxicity*; D002470:Cell Survival / Q000187:drug effects / Q000502:physiology; D002478:Cells, Cultured; D005260:Female; D006321:Heart / Q000187:drug effects / Q000254:growth & development; D006339:Heart Rate / Q000187:drug effects* / Q000502:physiology; D008297:Male; D009200:Myocardial Contraction / Q000187:drug effects* / Q000502:physiology; D032383:Myocytes, Cardiac / Q000187:drug effects* / Q000502:physiology; D051381:Rats; D017208:Rats, Wistar
chemical_list: D002211:Capsaicin