title: Effect of Melatonin and Calmodulin in an Idiopathic Scoliosis Model.
abstract: Background. To explore influence of continuous illumination, luzindole, and Tamoxifen on incidence of scoliosis model of rats.Methods. Thirty-two one-month-old female rats were rendered into bipedal rats. The bipedal rats were divided into 4 groups: group A by intraperitoneal injection of luzindole and continuous illumination; group B by intraperitoneal injection of luzindole only; group C by intraperitoneal injection of luzindole and oral administration of Tamoxifen; and group D by intraperitoneal injection of equivalent saline. Radiographs were taken at 8th week and 16th week, and incidence and the Cobb angles of scoliosis were calculated. At 16th week, all rats were sacrificed. Before the sacrifice, the levels of calmodulin were measured in each group.Results. At 8th week, scoliosis occurred in groups A and B, with an incidence of 75% and 12.5%, respectively, while rats in group C or D had no scoliosis. At 16th week, scoliosis incidences in groups A and B were 57% and 62.5%, respectively. No scoliosis occurred in group C or D. Calmodulin in platelets in group B was significantly different, compared with groups A and D. There was no significant difference in calmodulin in platelets in groups B and C.Conclusion. By intraperitoneal injection of luzindole in bipedal rats, scoliosis rat models could be successfully made. Under light, incidence of scoliosis may be increased at an early period but it is reversible. Tamoxifen can suppress natural process of scoliosis.
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
authors.forename: Jun-Zhe
authors.initials: JZ
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
authors.forename: Wen-Hua
authors.identifier: 0000-0002-8419-5444
authors.initials: WH
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
authors.forename: Li-Jiang
authors.initials: LJ
authors.lastname: He
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Ke
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
authors.forename: Long
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Orthopaedics, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, Fujian 362000, China.
authors.forename: Zhang-Sheng
authors.initials: ZS
authors.lastname: Dai
authors.affiliation: Department of Orthopaedics, Changhai Hospital, The Second Military Medical University, Shanghai 200433, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000818:Animals; D002147:Calmodulin / Q000378:metabolism*; D004195:Disease Models, Animal; D005260:Female; D006801:Humans; D008029:Lighting / Q000009:adverse effects; D008550:Melatonin / Q000378:metabolism; D051381:Rats; D012600:Scoliosis / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000188:drug therapy* / Q000503:physiopathology; D013629:Tamoxifen / Q000008:administration & dosage*; D014363:Tryptamines / Q000008:administration & dosage* / Q000009:adverse effects
chemical_list: D002147:Calmodulin; D014363:Tryptamines; D013629:Tamoxifen; C057154:luzindole; D008550:Melatonin