title: Electrospray ionization in concentrated acetonitrile vapor improves the performance of mass spectrometry for proteomic analyses.
abstract: Suppressing the background interferences and enhancing the analytes signals are long-term goals in high performance electrospray ionization mass spectrometry (ESI-MS) analyses. We observed that performing electrospray in the presence of a concentrated acetonitrile atmosphere suppresses background interferences and enhances peptide signals. An enclosed nanoESI source was utilized to provide a stable atmosphere of concentrated acetonitrile vapor for high performance ESI-MS analyses. The median MS signal intensity increased by 5 times for a set of 23 BSA tryptic peptides in direct ESI-MS analysis. Further, the number of reproducibly and precisely quantified peptides could be improved 67% in six replicate label-free quantitative proteome analyses by this strategy.
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Chen
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China. Electronic address: wangfj@dicp.ac.cn.
authors.forename: Fangjun
authors.initials: F
authors.lastname: Wang
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Zheyi
authors.initials: Z
authors.lastname: Liu
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China; University of Chinese Academy of Sciences, Beijing 100049, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Liu
authors.affiliation: Haochuang Biotech. Co. Ltd., Hangzhou 311121, Zhejiang, PR China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China.
authors.forename: Yukui
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: CAS Key Laboratory of Separation Sciences for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Dalian 116023, Liaoning, PR China.
authors.forename: Hanfa
authors.initials: H
authors.lastname: Zou
mesh_terms: D000097:Acetonitriles / Q000737:chemistry*; D000595:Amino Acid Sequence; D000818:Animals; D002417:Cattle; D002853:Chromatography, Liquid; D005740:Gases / Q000737:chemistry; D006801:Humans; D007477:Ions; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D020543:Proteome / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D012710:Serum Albumin, Bovine / Q000378:metabolism; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D053719:Tandem Mass Spectrometry; D014835:Volatilization
chemical_list: D000097:Acetonitriles; D005740:Gases; D007477:Ions; D010455:Peptides; D020543:Proteome; D012710:Serum Albumin, Bovine; C032159:acetonitrile
keywords: Acetonitrile; Background interference; Electrospray ionization; Mass spectrometry