title: Association of CCND1 Gene c.870G>A Polymorphism with Breast Cancer Risk: A Case-ControlStudy and a Meta-Analysis.
abstract: Cyclin D1 (CCND1) plays an essential role in regulating the progress of the cell cycle from G1 to S phase. There is a common c.870G>A polymorphism in the CCND1 gene. The aim of this study was to investigate the association of CCND1 gene c.870G>A polymorphism with breast cancer risk in a case-control study, which followed by a meta-analysis and an in silico analysis. Three hundred and thirty-five subjects composed of 174 women with breast cancer and 161 healthy controls were included in the case-control study. CCND1 gene c.870G>A genotyping was performed by PCR-RFLP. Meta-analysis was done for 14 studies composed of 7281 cases and 6820 controls. Some bioinformatics tools were applied to investigate the effects of c.870G>A on the mRNA splicing and structure. Our data obtained from case-control study revealed that GA genotype (OR: 1.89, 95%CI: 1.12-3.17, p = 0.017), AA genotype (OR: 1.95, 95%CI: 1.08-3.53, p = 0.027), and A allele (OR: 1.44, 95%CI: 1.06-1.95, p = 0.019) were significantly associated with breast cancer risk. The results of meta-analysis showed a significant association between CCND1 c.870G>A polymorphism and breast cancer risk, especially in Caucasian population. In silico analysis revealed that c.870G>A transition affect CCND1 mRNA splicing and secondary structure.
authors.affiliation: Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Soleimani
authors.affiliation: Trauma Research Center, Kashan University of Medical Sciences, Kashan, Iran. kheirkhahdavood@gmail.com.|Department of Pediatrics, Kashan University of Medical Sciences, Kashan, Iran. kheirkhahdavood@gmail.com.
authors.forename: Davood
authors.initials: D
authors.lastname: Kheirkhah
authors.affiliation: Autoimmune Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Mohammad Reza
authors.initials: MR
authors.lastname: Sharif
authors.affiliation: Infectious Diseases Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Sharif
authors.affiliation: Gametogenesis Research Center, Kashan University of Medical Sciences, Kashan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Karimian
authors.affiliation: Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
authors.forename: Younes
authors.initials: Y
authors.lastname: Aftabi
mesh_terms: D000483:Alleles; D001943:Breast Neoplasms / Q000235:genetics*; D016022:Case-Control Studies; D019938:Cyclin D1 / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008875:Middle Aged; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D012307:Risk Factors
chemical_list: C530104:CCND1 protein, human; D019938:Cyclin D1
keywords: Breast cancer; CCND1 gene; Genetic polymorphism; Meta-analysis; c.870G>A