title: Correlation of cell surface proteins of distinct Beauveria bassiana cell types and adaption to varied environment and interaction with the host insect.
abstract: The insect fungal pathogen Beauveria bassiana produces a number of distinct cell types that include aerial conidia, blastospores and haemolymph-derived cells, termed hyphal bodies, to adapt varied environment niches and within the host insect. These cells display distinct biochemical properties and surface structures, and a highly ordered outermost brush-like structure uniquely present on hyphal bodies, but not on any in vitro cells. Here, we found that the outermost structure on the hyphal bodies mainly consisted of proteins associated to structural wall components in that most of it could be removed by dithiothreitol (DTT) or proteinase K. DTT-treatment also caused delayed germination, decreased tolerance to ultraviolet irradiation and virulence of conidia or blastospores, with decreased adherence and alternated carbohydrate epitopes, suggesting involvement in fungal development, stress responses and virulence. To characterize these cell surface molecules, proteins were released from the living cells using DTT, and identified and quantitated using label-free quantitative mass spectrometry. Thereafter, a series of bioinformatics programs were used to predict cell surface-associated proteins (CSAPs), and 96, 166 and 54 CSAPs were predicted from the identified protein pools of conidia, blastospores and hyphal bodies, respectively, which were involved in utilization of carbohydrate, nitrogen, and lipid, detoxification, pathogen-host interaction, and likely other cellular processes. Thirteen, sixty-nine and six CSAPs were exclusive in conidia, blastospores and hyphal bodies, respectively, which were verified by eGFP-tagged proteins at their N-terminus. Our data provide a crucial cue to understand mechanism of B. bassiana to adapt to varied environment and interaction with insect host.
authors.affiliation: College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Chongqing Key Laboratory of Child Infection and Immunity, Pediatric Research Institute, Children's Hospital of Chongqing Medical University, Chongqing 400014, People's Republic of China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Zhuoyue
authors.initials: Z
authors.lastname: Lu
authors.affiliation: College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Zhibing
authors.initials: Z
authors.lastname: Luo
authors.affiliation: College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Xuebing
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing 400715, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: College of Plant Protection, Biotechnology Research Center, Southwest University, Chongqing 400715, People's Republic of China. Electronic address: yjzhang@swu.edu.cn.
authors.forename: Yongjun
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D000818:Animals; D052982:Beauveria / Q000235:genetics* / Q000472:pathogenicity; D019070:Cell Lineage / Q000235:genetics; D002462:Cell Membrane / Q000235:genetics; D059647:Gene-Environment Interaction; D054884:Host-Pathogen Interactions / Q000235:genetics*; D025301:Hyphae / Q000235:genetics* / Q000254:growth & development; D007313:Insecta / Q000382:microbiology; D008565:Membrane Proteins / Q000096:biosynthesis / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics
chemical_list: D008565:Membrane Proteins
keywords: Beauveria bassiana; Blastospores; Cell surface-associated protein; Conidia; Hyphal bodies; Pathogen-host interaction