title: Independent metal-thiolate cluster formation in C-terminal Cys-rich region of a rice type 1 metallothionein isoform.
abstract: In this study we examined the independent self assembly of metal-binding in C-terminal Cys- rich region of a type 1 metallothionein (MT) isoform from rice (OsMTI-1b). To this end the N-terminal of OsMTI-1b (C-OsMTI-1b) was heterologously expressed in Escherichia coli as fusion protein with glutathione-S-transferase (GST). As compared with control (The E. coli cells containing pET41a without gene), transgenic E. coli cells expressing GST-C-OsMTI-1b accumulated more Ni2+, Cd2+, and Zn2+from culture medium and showed increased tolerance against these metals. The recombinant GST-C-OsMTI-1b was purified using affinity chromatography. According to in vitro assays the protein GST-C-OsMTI-1b was able to form complexes with Ni2+, Cd2+and Zn2+. These results demonstrate the formation of independent metal-thiolate cluster at C-terminal Cys-rich region of OsMTI-1b without participation of N-terminal Cys-rich region.
authors.affiliation: Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: Rahim
authors.initials: R
authors.lastname: Malekzadeh
authors.affiliation: Department of Biotechnology, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: a.shahpiri@cc.iut.ac.ir.
authors.forename: Azar
authors.initials: A
authors.lastname: Shahpiri
mesh_terms: D000595:Amino Acid Sequence; D003001:Cloning, Molecular; D003545:Cysteine; D008668:Metallothionein / Q000737:chemistry* / Q000235:genetics; D009154:Mutation; D009942:Organometallic Compounds / Q000737:chemistry*; D012275:Oryza; D010940:Plant Proteins / Q000737:chemistry*; D055550:Protein Stability
chemical_list: D009942:Organometallic Compounds; D010940:Plant Proteins; C407523:metallothionein isoform 1; D008668:Metallothionein; D003545:Cysteine
keywords: C-terminal Cys-rich region; Metal-thiolate cluster; Metallothionein; OsMTI-1b; Rice