title: High-speed photoacoustic microscopy of mouse cortical microhemodynamics.
abstract: We applied high-speed photoacoustic microscopy (PAM) for both cortical microenvironment studies and dynamic brain studies, with micrometer-level optical resolution and a millisecond-level cross-sectional imaging speed over a millimeter-level field of view. We monitored blood flow redistribution in mini-stroke mouse models and cerebral autoregulation induced by a vasoactive agent. Our results collectively suggest that high-speed PAM is a promising tool for understanding dynamic neurophysiological phenomena, complementing conventional imaging modalities.
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
authors.forename: Li
authors.initials: L
authors.lastname: Lin
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.|Department of Biomedical Engineering, Duke University, Durham, NC, USA.
authors.forename: Junjie
authors.initials: J
authors.lastname: Yao
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
authors.forename: Ruiying
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: Chun-Cheng
authors.initials: CC
authors.lastname: Chen
authors.affiliation: Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, USA.
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Huang
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.|Department of Mechanical and Biomedical Engineering, City University of Hong Kong, Hong Kong.
authors.forename: Lidai
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Surgery, Washington University School of Medicine, St. Louis, MO, USA.
authors.forename: William
authors.initials: W
authors.lastname: Chapman
authors.affiliation: Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX, USA.
authors.forename: Jun
authors.initials: J
authors.lastname: Zou
authors.affiliation: Optical Imaging Laboratory, Department of Biomedical Engineering, Washington University in St. Louis, St. Louis, MO, USA.
authors.forename: Lihong V
authors.initials: LV
authors.lastname: Wang
mesh_terms: D000818:Animals; D001921:Brain / Q000000981:diagnostic imaging*; D005260:Female; D051379:Mice; D008853:Microscopy; D061088:Photoacoustic Techniques; D013057:Spectrum Analysis
keywords: MEMS scanning mirror; blood flow redistribution; cerebral autoregulation; cortical hemodynamics; microhemorrhage; photoacoustic microscopy