title: First description of Cryptosporidium hominis GP60 genotype IkA20G1 and Cryptosporidium parvum GP60 genotypes IIaA18G3R1 and IIaA15G2R1 in foals in Brazil.
abstract: The present study focuses on Cryptosporidium infections of foals in Brazil. A total of 92 animals of different breeds from 11 farms in the vicinity of Araçatuba in the state of São Paulo, were examined. According to PCR targeting the 18S rRNA gene, Cryptosporidium sp. DNA was detected in 21.7% (20/92) of foals. Good quality 18S rRNA, actin, HSP70 and gp60 genes nPCR amplicons were obtained from five fecal samples. PCR amplification and sequencing of a fragment of the GP60 sporozoite surface glycoprotein gene revealed C. parvum genotypes IIaA18G3R1, IIaA15G2R1. Interestingly, we also detected in two foals a GP60 genotype related to the human parasite C. hominis.
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: sandra_byol@yahoo.com.br.
authors.forename: Sandra Valéria
authors.initials: SV
authors.lastname: Inácio
authors.affiliation: Department of Infectious Disease & Global Health, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA, USA. Electronic address: giovanni.widmer@tufts.edu.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Widmer
authors.affiliation: Universidade Estadual Paulista (UNESP), de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, São Paulo, Brazil. Electronic address: rolomonte@gmail.com.
authors.forename: Roberta Lomonte Lemos
authors.initials: RL
authors.lastname: de Brito
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: anaszucatto@hotmail.com.
authors.forename: Anaiza Simão
authors.initials: AS
authors.lastname: Zucatto
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: monallyaquino@hotmail.com.
authors.forename: Monally Conceição Costa
authors.initials: MC
authors.lastname: de Aquino
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: bruno.9988@hotmail.com.
authors.forename: Bruno César Miranda
authors.initials: BC
authors.lastname: Oliveira
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, São Paulo, Brazil. Electronic address: akiravt@gmail.com.
authors.forename: Alex Akira
authors.initials: AA
authors.lastname: Nakamura
authors.affiliation: Universidade Estadual Paulista (UNESP), de Ciências Agrárias e Veterinárias, Departamento de Medicina Veterinária Preventiva e Reprodução Animal, Jaboticabal, São Paulo, Brazil. Electronic address: luiz.silveira@gmail.com.
authors.forename: Luiz da Silveira
authors.initials: LD
authors.lastname: Neto
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, São Paulo, Brazil. Electronic address: jgbcarvalho@uol.com.br.
authors.forename: João Gabriel Balizardo
authors.initials: JG
authors.lastname: Carvalho
authors.affiliation: Universidade Estadual de Campinas (Unicamp), Institutos de Biologia e Computação, Campinas, São Paulo, Brazil. Electronic address: jgomes@ic.unicamp.br.
authors.forename: Jancarlo Ferreira
authors.initials: JF
authors.lastname: Gomes
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Clínica, Cirurgia e Reprodução Animal, Araçatuba, São Paulo, Brazil. Electronic address: marcelo@fmva.unesp.br.
authors.forename: Marcelo Vasconcelos
authors.initials: MV
authors.lastname: Meireles
authors.affiliation: Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária, Araçatuba, Departamento de Apoio, Produção e Saúde Animal, Araçatuba, São Paulo, Brazil. Electronic address: bresciani@fmva.unesp.br.
authors.forename: Katia Denise Saraiva
authors.initials: KD
authors.lastname: Bresciani
mesh_terms: D000818:Animals; D001938:Brazil; D003457:Cryptosporidiosis / Q000469:parasitology*; D003458:Cryptosporidium / Q000235:genetics*; D016785:Cryptosporidium parvum / Q000235:genetics*; D016054:DNA, Protozoan / Q000235:genetics*; D005838:Genotype; D018840:HSP70 Heat-Shock Proteins / Q000235:genetics; D006736:Horses; D012337:RNA, Ribosomal, 18S / Q000235:genetics
chemical_list: D016054:DNA, Protozoan; D018840:HSP70 Heat-Shock Proteins; D012337:RNA, Ribosomal, 18S
keywords: Equine; Molecular biology; Parasite