title: miR-124 promotes the neuronal differentiation of mouse inner ear neural stem cells.
abstract: MicroRNAs (miRNAs or miRs) act as key regulators in neuronal development, synaptic morphogenesis and plasticity. However, their role in the neuronal differentiation of inner ear neural stem cells (NSCs) remains unclear. In this study, 6 miRNAs were selected and their expression patterns during the neuronal differentiation of inner ear NSCs were examined by RT-qPCR. We demonstrated that the culture of spiral ganglion stem cells present in the inner ears of newborn mice gave rise to neurons in vitro. The expression patterns of miR‑124, miR‑132, miR‑134, miR‑20a, miR‑17-5p and miR‑30a-5p were examined during a 14-day neuronal differentiation period. We found that miR‑124 promoted the neuronal differentiation of and neurite outgrowth in mouse inner ear NSCs, and that the changes in the expression of tropomyosin receptor kinase B (TrkB) and cell division control protein 42 homolog (Cdc42) during inner ear NSC differentiation were associated with miR‑124 expression. Our findings indicate that miR‑124 plays a role in the neuronal differentiation of inner ear NSCs. This finding may lead to the development of novel strategies for restoring hearing in neurodegenerative diseases.
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Di
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Du
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zong
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Chang
authors.initials: C
authors.lastname: Dong
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Kaitian
authors.initials: K
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Chen
authors.affiliation: Department of Otolaryngology, The First Affiliated Hospital, and Institute of Otorhinolaryngology, Sun Yat-sen University, Guangzhou, Guangdong 510080, P.R. China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D002454:Cell Differentiation / Q000235:genetics; D049109:Cell Proliferation; D002469:Cell Separation; D002478:Cells, Cultured; D007758:Ear, Inner / Q000166:cytology*; D005786:Gene Expression Regulation; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D058953:Neural Stem Cells / Q000166:cytology* / Q000378:metabolism; D016501:Neurites / Q000378:metabolism; D009474:Neurons / Q000166:cytology* / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D020813:Receptor, trkB / Q000378:metabolism; D018874:Spheroids, Cellular / Q000166:cytology; D014162:Transfection; D014404:Tubulin / Q000378:metabolism; D015854:Up-Regulation; D020764:cdc42 GTP-Binding Protein / Q000378:metabolism
chemical_list: D035683:MicroRNAs; C540079:Mirn124 microRNA, mouse; D014404:Tubulin; C462941:beta3 tubulin, mouse; D020813:Receptor, trkB; D020764:cdc42 GTP-Binding Protein