title: Transcriptome analysis reveals common differential and global gene expression profiles in bluetongue virus serotype 16 (BTV-16) infected peripheral blood mononuclear cells (PBMCs) in sheep and goats.
abstract: Bluetongue is an economically important infectious, arthropod borne viral disease of domestic and wild ruminants, caused by Bluetongue virus (BTV). Sheep are considered the most susceptible hosts, while cattle, buffalo and goats serve as reservoirs. The viral pathogenesis of BTV resulting in presence or absence of clinical disease among different hosts is not clearly understood. In the present study, transcriptome of sheep and goats peripheral blood mononuclear cells infected with BTV-16 was explored. The differentially expressed genes (DEGs) identified were found to be significantly enriched for immune system processes - NFκB signaling, MAPK signaling, Ras signaling, NOD signaling, RIG signaling, TNF signaling, TLR signaling, JAK-STAT signaling and VEGF signaling pathways. Greater numbers of DEGs were found to be involved in immune system processes in goats than in sheep. Interestingly, the DEHC (differentially expressed highly connected) gene network was found to be dense in goats than in sheep. Majority of the DEHC genes in the network were upregulated in goats but down-regulated in sheep. The network of differentially expressed immune genes with the other genes further confirmed these findings. Interferon stimulated genes - IFIT1 (ISG56), IFIT2 (ISG54) and IFIT3 (ISG60) responsible for antiviral state in the host were found to be upregulated in both the species. STAT2 was the TF commonly identified to co-regulate the DEGs, with its network showing genes that are downregulated in sheep but upregulated in goats. The genes dysregulated and the networks perturbed in the present study indicate host variability with a positive shift in immune response to BTV in goats than in sheep.
authors.affiliation: Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India; Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Singh
authors.affiliation: Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, India.
authors.forename: Minakshi
authors.initials: M
authors.lastname: Prasad
authors.affiliation: Division of Biological Products, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122.
authors.forename: Bina
authors.initials: B
authors.lastname: Mishra
authors.affiliation: Oomens Lab, Division of Veterinary Pathobiology, CVHS, OSU, Stillwater, OK, USA.
authors.forename: Siddappa
authors.initials: S
authors.lastname: Manjunath
authors.affiliation: Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India.
authors.forename: Amit Ranjan
authors.initials: AR
authors.lastname: Sahu
authors.affiliation: Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India; Division of Bacteriology and Mycology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly UP-243122, India.
authors.forename: G
authors.initials: G
authors.lastname: Bhuvana Priya
authors.affiliation: Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India.
authors.forename: Sajad Ahmad
authors.initials: SA
authors.lastname: Wani
authors.affiliation: Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India; Molecular Biology Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India.
authors.forename: Aditya Prasad
authors.initials: AP
authors.lastname: Sahoo
authors.affiliation: Division of Animal Genetics, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Kumar
authors.affiliation: Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, India.
authors.forename: Shweta
authors.initials: S
authors.lastname: Balodi
authors.affiliation: Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary & Animal Sciences, Hisar, India.
authors.forename: Anupama
authors.initials: A
authors.lastname: Deora
authors.affiliation: Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India.
authors.forename: Shikha
authors.initials: S
authors.lastname: Saxena
authors.affiliation: Computational Biology and Genomics Facility Lab, Division of Veterinary Biotechnology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly, UP-243122, India.
authors.forename: Ravi Kumar
authors.initials: RK
authors.lastname: Gandham
keywords: Bluetongue; Bluetongue virus serotype-16; DEGs; DEHC genes; PBMCs; RNA-seq; transcriptome