title: Periodic imidazolium-bridged hybrid monolith for high-efficiency capillary liquid chromatography with enhanced selectivity.
abstract: A novel periodic imidazolium-bridged hybrid monolithic column was developed. With diene imidazolium ionic liquid 1-allyl-3-vinylimidazolium bromide as both cross-linker and organic functionalized reagent, a new periodic imidazolium-bridged hybrid monolithic column was facilely prepared in capillary with homogeneously distributed cationic imidazolium by a one-step free-radical polymerization with polyhedral oligomeric silsesquioxane methacryl substituted. The successful preparation of the new column was verified by Fourier transform infrared spectroscopy, scanning electron microscopy, elemental analysis, and surface area analysis. Most interestingly, the bonded amount of 1-allyl-3-vinylimidazolium bromide of the new column is three times higher than that of the conventional imidazolium-embedded hybrid monolithic column and the specific surface area of the column reached 478 m2/g. The new column exhibited high stability, excellent separation efficiency, and enhanced separation selectivity. The column efficiency reached 151 000 plates/m for alkylbenzenes. Furthermore, the new column was successfully used for separation of highly polar nucleosides and nucleic acid bases with pure water as mobile phase and even bovine serum albumin tryptic digest. All these results demonstrate the periodic imidazolium-bridged hybrid monolithic column is a good separation media and can be used for chromatographic separation of small molecules and complex biological samples with high efficiency.
authors.affiliation: College of Pharmaceutical Sciences, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Xiaoqiang
authors.initials: X
authors.lastname: Qiao
authors.affiliation: College of Pharmaceutical Sciences, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Niu
authors.initials: N
authors.lastname: Zhang
authors.affiliation: College of Pharmaceutical Sciences, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Manman
authors.initials: M
authors.lastname: Han
authors.affiliation: College of Pharmaceutical Sciences, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China.
authors.forename: Xueyun
authors.initials: X
authors.lastname: Li
authors.affiliation: Chemical Biology Key Laboratory of Hebei Province, College of Chemistry & Environmental Science, Hebei University, Baoding, China.
authors.forename: Xinying
authors.initials: X
authors.lastname: Qin
authors.affiliation: College of Chemistry & Environmental Science, Hebei University, Baoding, China.
authors.forename: Shigang
authors.initials: S
authors.lastname: Shen
mesh_terms: D002853:Chromatography, Liquid; D005609:Free Radicals; D007093:Imidazoles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009705:Nucleosides / Q000302:isolation & purification*; D058105:Polymerization; D012710:Serum Albumin, Bovine / Q000737:chemistry; D014753:Vinyl Compounds / Q000737:chemistry*
chemical_list: D005609:Free Radicals; D007093:Imidazoles; D009705:Nucleosides; D014753:Vinyl Compounds; D012710:Serum Albumin, Bovine
keywords: high-efficiency separation; liquid chromatography; monolithic column; periodic imidazolium bridged