title: Liposomes for PET and MR Imaging and for Dual Targeting (Magnetic Field/Glucose Moiety): Synthesis, Properties, and in Vivo Studies.
abstract: We describe the potentiality of a new liposomal formulation enabling positron emission tomography (PET) and magnetic resonance MR() imaging. The bimodality is achieved by coupling a68Ga-based radiotracer on the bilayer of magnetic liposomes. In order to enhance the targeting properties obtained under a permanent magnetic field, a sugar moiety was added in the lipid formulation. Two new phospholipids were synthesized, one with a specific chelator of68Ga (DSPE-PEG-NODAGA) and one with a glucose moiety (DSPE-PEG-glucose). The liposomes were produced according to a fast and safe process, with a high radiolabeling yield. MR and PET imaging were performed on mice bearing human glioblastoma tumors (U87MG) after iv injection. The accumulation of the liposomes in solid tumor is evidenced by MR imaging and the amount is evaluated in vivo and ex vivo according to PET imaging. An efficient magnetic targeting is achieved with these new magnetic liposomes.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8234, PHENIX , F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8232, IPCM , F-75005 Paris, France.
authors.forename: Jérémy
authors.initials: J
authors.lastname: Malinge
authors.affiliation: Laboratoire d'Imagerie Moléculaire Positonique (LIMP), UMS028 Phénotypage du petit animal, UPMC Univ Paris 06, Paris, France.|Médecine nucléaire et radiopharmacie, Hôpital Tenon, AP-HP , Paris, France.
authors.forename: Bastien
authors.initials: B
authors.lastname: Géraudie
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8234, PHENIX , F-75005 Paris, France.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8232, IPCM , F-75005 Paris, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Savel
authors.affiliation: Médecine nucléaire et radiopharmacie, Hôpital Tenon, AP-HP , Paris, France.
authors.forename: Valérie
authors.initials: V
authors.lastname: Nataf
authors.affiliation: Laboratoire d'Imagerie Moléculaire Positonique (LIMP), UMS028 Phénotypage du petit animal, UPMC Univ Paris 06, Paris, France.
authors.forename: Aurélie
authors.initials: A
authors.lastname: Prignon
authors.affiliation: Laboratoire d'Imagerie Moléculaire Positonique (LIMP), UMS028 Phénotypage du petit animal, UPMC Univ Paris 06, Paris, France.
authors.forename: Claire
authors.initials: C
authors.lastname: Provost
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8232, IPCM , F-75005 Paris, France.
authors.forename: Yongmin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Université Paris Diderot, Plateforme de recherche préclinique FRIM , 46 rue Henri Huchard, 75018 Paris, France.
authors.forename: Phalla
authors.initials: P
authors.lastname: Ou
authors.affiliation: Médecine nucléaire et radiopharmacie, Hôpital Tenon, AP-HP , Paris, France.
authors.forename: Khaldoun
authors.initials: K
authors.lastname: Kerrou
authors.affiliation: Laboratoire d'Imagerie Moléculaire Positonique (LIMP), UMS028 Phénotypage du petit animal, UPMC Univ Paris 06, Paris, France.|Médecine nucléaire et radiopharmacie, Hôpital Tenon, AP-HP , Paris, France.
authors.forename: Jean-Noël
authors.initials: JN
authors.lastname: Talbot
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8234, PHENIX , F-75005 Paris, France.
authors.forename: Jean-Michel
authors.initials: JM
authors.lastname: Siaugue
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8232, IPCM , F-75005 Paris, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Sollogoub
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 8234, PHENIX , F-75005 Paris, France.
authors.forename: Christine
authors.identifier: 0000-0002-4080-3508
authors.initials: C
authors.lastname: Ménager
mesh_terms: D000085:Acetates / Q000737:chemistry; D000818:Animals; D045744:Cell Line, Tumor; D002626:Chemistry, Pharmaceutical / Q000379:methods; D005260:Female; D005909:Glioblastoma / Q000175:diagnosis; D005947:Glucose / Q000737:chemistry*; D006573:Heterocyclic Compounds, 1-Ring / Q000737:chemistry; D006801:Humans; D008055:Lipids / Q000737:chemistry; D008081:Liposomes / Q000737:chemistry*; D060526:Magnetic Fields; D008279:Magnetic Resonance Imaging / Q000379:methods; D051379:Mice; D008819:Mice, Nude; D010714:Phosphatidylethanolamines / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D011092:Polyethylene Glycols / Q000737:chemistry; D049268:Positron-Emission Tomography / Q000379:methods
chemical_list: C572723:1-(1,3-carboxypropyl)-4,7-carboxymethyl-1,4,7-triazacyclononane; D000085:Acetates; D006573:Heterocyclic Compounds, 1-Ring; D008055:Lipids; D008081:Liposomes; D010714:Phosphatidylethanolamines; D010743:Phospholipids; C038089:1,2-distearoylphosphatidylethanolamine; D011092:Polyethylene Glycols; D005947:Glucose
keywords: MRI; PET; Warburg effect; gallium; liposomes; magnetic nanoparticles; magnetic targeting