title: Biological evaluation of phytoconstituents from Polygonum hydropiper.
abstract: Fourteen compounds including vanicoside B (1), vanicoside F (2), vanicoside E (3) and 5,6-dehydrokawain (4), aniba-dimer-A (5), 6,6'-((1α,2α,3β,4β)-2,4-diphenylcyclobutane-1,3-diyl)bis(4-methoxy-2H-pyran-2-one) (6), (+)-ketopinoresinol (7), isorhamnetin (8), 3,7-dihydroxy-5,6-dimethoxy-flavone (9), isalpinin (10), cardamomin (11), pinosylvin (12), 2-desoxy-4-epi-pulchellin (13) and β-sitosterol (14) were isolated from dichloromethane-soluble portion of Polygonum hydropiper. By using Alamar blue assay, compounds 2, 7, 8, 11 and 12 were found to be active against Trypanosoma brucei with IC50values in the range of 0.49-7.77 μg/mL. Cardamomin (11) had most significant activity against T. brucei with IC50/IC90values of 0.49/0.81 μg/mL compared to the positive control DFMO (IC50/IC90: 3.02/8.05 μg/mL). Furthermore, in antimalarial, antimicrobial, anti-inflammatory, PPAR and cytotoxic assays, some compounds have demonstrated moderate inhibitory potentials.
authors.affiliation: a TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy , Hunan University of Chinese Medicine , Changsha , P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xiao
authors.affiliation: b National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy , The University of Mississippi , Oxford , MS , USA.
authors.forename: Ranga
authors.initials: R
authors.lastname: Rao Ravu
authors.affiliation: b National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy , The University of Mississippi , Oxford , MS , USA.|c Department of Biomolecular Sciences, School of Pharmacy , The University of Mississippi , MS , USA.
authors.forename: Babu L
authors.initials: BL
authors.lastname: Tekwani
authors.affiliation: a TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy , Hunan University of Chinese Medicine , Changsha , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: a TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy , Hunan University of Chinese Medicine , Changsha , P.R. China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Liu
authors.affiliation: b National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy , The University of Mississippi , Oxford , MS , USA.
authors.forename: Melissa R
authors.initials: MR
authors.lastname: Jacob
authors.affiliation: b National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy , The University of Mississippi , Oxford , MS , USA.|c Department of Biomolecular Sciences, School of Pharmacy , The University of Mississippi , MS , USA.
authors.forename: Shabana I
authors.initials: SI
authors.lastname: Khan
authors.affiliation: d Hunan Provincial Key Laboratory of Diagnostic and Therapeutic Research in Chinese Medicine , Hunan University of Chinese Medicine , Changsha , P.R. China.
authors.forename: Xiong
authors.initials: X
authors.lastname: Cai
authors.affiliation: a TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy , Hunan University of Chinese Medicine , Changsha , P.R. China.
authors.forename: Cai-Yun
authors.initials: CY
authors.lastname: Peng
authors.affiliation: b National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy , The University of Mississippi , Oxford , MS , USA.|c Department of Biomolecular Sciences, School of Pharmacy , The University of Mississippi , MS , USA.
authors.forename: Ikhlas A
authors.initials: IA
authors.lastname: Khan
authors.affiliation: b National Center for Natural Products Research, Research Institute of Pharmaceutical Sciences, School of Pharmacy , The University of Mississippi , Oxford , MS , USA.|c Department of Biomolecular Sciences, School of Pharmacy , The University of Mississippi , MS , USA.
authors.forename: Xing-Cong
authors.initials: XC
authors.lastname: Li
authors.affiliation: a TCM and Ethnomedicine Innovation & Development Laboratory, Sino-Luxemburg TCM Research Center, School of Pharmacy , Hunan University of Chinese Medicine , Changsha , P.R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology; D000962:Antimalarials / Q000737:chemistry / Q000494:pharmacology*; D047188:Chalcones / Q000494:pharmacology; D002934:Cinnamates / Q000494:pharmacology; D004353:Drug Evaluation, Preclinical / Q000379:methods; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D047495:PPAR gamma / Q000235:genetics; D064209:Phytochemicals / Q000737:chemistry / Q000494:pharmacology; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D028502:Polygonum / Q000737:chemistry*; D012855:Sitosterols / Q000494:pharmacology; D014344:Trypanocidal Agents / Q000737:chemistry / Q000494:pharmacology*; D014346:Trypanosoma brucei brucei / Q000187:drug effects*
chemical_list: D000890:Anti-Infective Agents; D000894:Anti-Inflammatory Agents, Non-Steroidal; D000962:Antimalarials; D047188:Chalcones; D002934:Cinnamates; D047495:PPAR gamma; D064209:Phytochemicals; D010936:Plant Extracts; D012855:Sitosterols; D014344:Trypanocidal Agents; C087106:vanicoside B; C025473:gamma-sitosterol; C436747:cardamonin
keywords: Polygonaceae; Polygonum hydropiper; antitrypanosomal; bioassay