title: Cross-reactivities of mammalian MAPKs antibodies in rotifer and copepod: Application in mechanistic studies in aquatic ecotoxicology.
abstract: The mitogen-activated protein kinases (MAPKs) family is known to mediate various biological processes in response to diverse environmental pollutants. Although MAPKs are well characterized and studied in vertebrates, in invertebrates the cross-reactivities of MAPKs antibodies were not clearly known in response to environmental pollutants due to limited information of antibody epitopes with material resources for invertebrates. In this paper, we performed phylogenetic analysis of MAPKs genes in the marine rotifer Brachionus koreanus and the copepods Paracyclopina nana and Tigriopus japonicus. Also in rotifer and copepods, several studies of Western blot of MAPK signaling pathways were shown in response to environmental pollutants, including multi-walled carbon nanotubes (MWCNTs), water-accommodated fractions (WAFs) of crude oil, and microplastics. This paper will provide a better understanding of the underlying mechanistic scenario in terms of cross-reactivities of mammalian antibodies in rotifer and copepod.
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Hye-Min
authors.initials: HM
authors.lastname: Kang
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea; Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 04763, South Korea.
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Jeong
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Young Hwan
authors.initials: YH
authors.lastname: Lee
authors.affiliation: Department of Life Sciences, College of Natural Sciences, Hanyang University, Seoul 04763, South Korea.
authors.forename: Yan-Hong
authors.initials: YH
authors.lastname: Cui
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Duck-Hyun
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Min-Chul
authors.initials: MC
authors.lastname: Lee
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Hui-Su
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Jeonghoon
authors.initials: J
authors.lastname: Han
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Dae-Sik
authors.initials: DS
authors.lastname: Hwang
authors.affiliation: Department of Life Sciences, College of Natural Sciences, Hanyang University, Seoul 04763, South Korea.
authors.forename: Su-Jae
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea. Electronic address: jslee2@skku.edu.
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
mesh_terms: D000818:Animals; D000906:Antibodies / Q000276:immunology; D015153:Blotting, Western; D033342:Copepoda / Q000235:genetics / Q000276:immunology*; D003429:Cross Reactions; D054750:Ecotoxicology / Q000379:methods*; D015971:Gene Expression Regulation, Enzymologic; D008322:Mammals / Q000276:immunology; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics* / Q000276:immunology*; D037742:Nanotubes, Carbon / Q000633:toxicity; D010578:Petroleum / Q000378:metabolism; D010802:Phylogeny; D010969:Plastics / Q000633:toxicity; D012403:Rotifera / Q000235:genetics / Q000276:immunology*; D014874:Water Pollutants, Chemical / Q000633:toxicity
chemical_list: D000906:Antibodies; D037742:Nanotubes, Carbon; D010578:Petroleum; D010969:Plastics; D014874:Water Pollutants, Chemical; D020928:Mitogen-Activated Protein Kinases
keywords: Copepod; Ecotoxicology; MAPKs; Rotifer; Signal transduction