title: Interspecies discrimination of A. fumigatus and siblings A. lentulus and A. felis of the Aspergillus section Fumigati using the AsperGenius®assay.
abstract: The AsperGenius®assay detects several Aspergillus species and the A. fumigatus Cyp51A mutations TR34/L98H/T289A/Y121F that are associated with azole resistance. We evaluated its contribution in identifying A. lentulus and A. felis, 2 rare but intrinsically azole-resistant sibling species within the Aspergillus section Fumigati. Identification of these species with conventional culture techniques is difficult and time-consuming. The assay was tested on (i) 2 A. lentulus and A. felis strains obtained from biopsy proven invasive aspergillosis and (ii) control A. fumigatus (n=3), A. lentulus (n=6) and A. felis species complex (n=12) strains. The AsperGenius®resistance PCR did not detect the TR34target in A. lentulus and A. felis in contrast to A. fumigatus. Melting peaks for L98H and Y121F markers differed and those of the Y121F marker were particularly suitable to discriminate the 3 species. In conclusion, the assay can be used to rapidly discriminate A. fumigatus, A. lentulus and A. felis.
authors.affiliation: Department of Internal Medicine, section of Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.. Electronic address: g.chong@erasmusmc.nl.
authors.forename: G M
authors.initials: GM
authors.lastname: Chong
authors.affiliation: Department of Medical Microbiology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: A G
authors.initials: AG
authors.lastname: Vonk
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, The Netherlands.; Department of Medical Microbiology, Radboudumc, Nijmegen, The Netherlands.
authors.forename: J F
authors.initials: JF
authors.lastname: Meis
authors.affiliation: PathoNostics B.V., Maastricht, The Netherlands.
authors.forename: G J H
authors.initials: GJ
authors.lastname: Dingemans
authors.affiliation: CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands.
authors.forename: J
authors.initials: J
authors.lastname: Houbraken
authors.affiliation: Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital (CWZ), Nijmegen, The Netherlands.
authors.forename: F
authors.initials: F
authors.lastname: Hagen
authors.affiliation: PathoNostics B.V., Maastricht, The Netherlands.
authors.forename: G R
authors.initials: GR
authors.lastname: Gaajetaan
authors.affiliation: PathoNostics B.V., Maastricht, The Netherlands.
authors.forename: D W E
authors.initials: DW
authors.lastname: van Tegelen
authors.affiliation: PathoNostics B.V., Maastricht, The Netherlands.
authors.forename: G F M
authors.initials: GF
authors.lastname: Simons
authors.affiliation: Department of Internal Medicine, section of Infectious Diseases, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: B J A
authors.initials: BJ
authors.lastname: Rijnders
mesh_terms: D000368:Aged; D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000175:diagnosis* / Q000188:drug therapy* / Q000382:microbiology; D001232:Aspergillus fumigatus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics; D001393:Azoles / Q000627:therapeutic use*; D003577:Cytochrome P-450 Enzyme System / Q000235:genetics*; D025141:Drug Resistance, Fungal / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics*; D006801:Humans; D008297:Male; D008826:Microbial Sensitivity Tests; D008875:Middle Aged
chemical_list: D000935:Antifungal Agents; D001393:Azoles; D005656:Fungal Proteins; D003577:Cytochrome P-450 Enzyme System; C465526:cytochrome P-450 CYP51A, Aspergillus
keywords: Aspergillus felis; Aspergillus fumigatus; Aspergillus lentulus; Cryptic species; Polymerase chain reaction; Sibling species