title: Nitrogen-doped graphene quantum dots-labeled epitope imprinted polymer with double templates via the metal chelation for specific recognition of cytochrome c.
abstract: A novel fluorescent sensor nitrogen-doped graphene quantum dots (N-GQDs)/SiO2/molecular imprinting polymer（N-GQDs/SiO2/MIP）was fabricated by surface imprinting and epitope imprinting to recognize and detect the target protein cytochrome c (Cyt C) with fluorescence quenching. In the polymerization process, the C- and N-terminal nonapeptides of Cyt C were selected as the double templates which were fixed by functional monomer (zinc acrylate) through metal chelation and steady six-membered ring. The linear range of fluorescence quenching for this receptor towards Cyt C was 0.20-60μM, and the detection limit was 0.11μM. The precision for six times replicate determination of Cyt C at 30μM was 1.20%, and the imprinting factor (IF) was 3.06. The recoveries of the material to Cyt C in urine were 99.3-114.0%. In brief, this work proposed a strategy to prepare a new type fluorescent imprinting polymer based on N-GQDs and provided an attractive perspective for the detection of protein by using the combination of N-GQDs and molecular imprinting technique.
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China.
authors.forename: Yun-Jing
authors.initials: YJ
authors.lastname: Yan
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China.
authors.forename: Xi-Wen
authors.initials: XW
authors.lastname: He
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China. Electronic address: wyli@nankai.edu.cn.
authors.forename: Wen-You
authors.initials: WY
authors.lastname: Li
authors.affiliation: College of Chemistry, Research Center for Analytical Sciences, State Key Laboratory of Medicinal Chemical Biology, Tianjin Key Laboratory of Biosensing and Molecular Recognition, Nankai University, Tianjin 300071, China; National Chromatographic Research and Analysis Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China. Electronic address: ykzhang@dicp.ac.cn.
authors.forename: Yu-Kui
authors.initials: YK
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D045304:Cytochromes c / Q000032:analysis / Q000652:urine*; D005456:Fluorescent Dyes / Q000737:chemistry; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D054802:Molecular Imprinting / Q000379:methods*; D009584:Nitrogen / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D012822:Silicon Dioxide / Q000737:chemistry; D013050:Spectrometry, Fluorescence / Q000379:methods
chemical_list: D005456:Fluorescent Dyes; D011108:Polymers; D012822:Silicon Dioxide; D006108:Graphite; D045304:Cytochromes c; D009584:Nitrogen
keywords: Epitope imprinting; Fluorescent sensor; Metal chelation; Nitrogen-doped graphene quantum dots; Surface imprinting