title: Matrix metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitor of matrix metalloproteinases (TIMP-1) and transforming growth factor-β2 (TGF-β2) expression in eutopic endometrium of women with peritoneal endometriosis.
abstract: The prevalence of endometriosis among reproductive age women is 7-17%; however, these figures reach 20-50% in patients suffering from infertility. Matrix metalloproteinases (MMPs) activity is thought to be particularly essential in the early phases of endometriosis development. Any changes in the equilibrium between MMPs activity and their tissue inhibitors (TIMPs) could be potentially harmful, promoting endometriosis development. The aim of this study was to investigate whether the MMP-2, MMP-9, TIMP-1 or TGF-B2 expression in eutopic endometrium from women with early endometriosis differ when compared with healthy subjects. The results were referred to the serum progesterone levels.
  
  
  
  Endometrial biopsy was taken from 42 patients (18 in the study group, 22 in thecontrol group) at the time of hysteroscopy for routine histology and for RT-PCR procedures. Comparison of the quantity of gene products was performed with a programme for densitometry and compared to GADPH product, which was a reference value.
  
  
  
  The obtained results did not reveal any statistical difference in endometrial expression of MMP-2, MMp-9, TIMP-1, and TGF-β2 or serum progesterone level between women with endometriosis and without visible signs of this illness.
  
  
  
  Despite the lack of statistical differences, it was observed that both examined metalloproteinases expressed a tendency to higher gene expression in the eutopic endometrium of women with endometriosis. However, both TIMP-1 and TGF-β2 expressions had the same tendency - higher values in endometriosis patients.
authors.affiliation: Department of Mother's and Child's Health, Poznań University of Medical Sciences, Poland.
authors.forename: Krzysztof
authors.initials: K
authors.lastname: Szymanowski
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproduction, Poznań University of Medical Sciences, Poland.
authors.forename: Mateusz
authors.initials: M
authors.lastname: Mikołajczyk
authors.affiliation: Department of Obstetrics and Gynecology, Division of Reproduction, Poznań University of Medical Sciences, Poland.
authors.forename: Przemysław
authors.initials: P
authors.lastname: Wirstlein
authors.affiliation: Department of Perinatology and Gynecology, Poznań University of Medical Sciences, Poland.
authors.forename: Anna
authors.initials: A
authors.lastname: Dera-Szymanowska
mesh_terms: D000328:Adult; D017364:Collagenases / Q000235:genetics* / Q000378:metabolism; D004715:Endometriosis / Q000201:enzymology / Q000235:genetics / Q000503:physiopathology*; D004717:Endometrium / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D019715:Tissue Inhibitor of Metalloproteinase-1 / Q000235:genetics* / Q000378:metabolism; D053781:Transforming Growth Factor beta2 / Q000235:genetics* / Q000378:metabolism; D055815:Young Adult
chemical_list: C560822:TIMP1 protein, human; D019715:Tissue Inhibitor of Metalloproteinase-1; D053781:Transforming Growth Factor beta2; D017364:Collagenases