title: Synergistic antibacterial effect of Bi2S3nanospheres combined with ineffective antibiotic gentamicin against methicillin-resistant Staphylococcus aureus.
abstract: In this paper, Bi2S3nanospheres with size of 212nm were prepared by a simple hydrothermal process. The selectively enhanced antibacterial effects of Bi2S3nanospheres with three classes of ineffective antibiotics, β-lactam (cefuroxime, CXM; cefotaxime, CTX and piperacillin, PIP), quinolone (ciprofloxacin, CIP) and aminoglycoside (gentamicin, GEN) against clinical isolated methicillin-resistant Staphylococcus aureus (MRSA) were investigated for the first time. GEN shows significantly synergistic growth inhibition against MRSA when combined with Bi2S3nanospheres, while CXM, CTX, PIP and CIP do not. Raman spectroscopy and Z potential studies reveal that Bi2S3could interact with GEN and the combination showed small electronegativity, which probably induced the increase of GEN content in cytoplasm of bacteria. Furthermore, the combination of Bi2S3nanospheres and GEN can destroy the bacterial membrane function and induce more bactericidal reactive oxygen generation than that of Bi2S3or GEN alone. The cytotoxicity test indicates that the combination of Bi2S3and GEN presented low toxicity to human normal hepatocyte L02. This work shows that Bi2S3nanospheres can be used to enhance the action of ineffective antibiotic GEN against MRSA, thus strengthening the antibiotic capacity for fighting MRSA infections.
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan, 430073, PR China.
authors.forename: Lulu
authors.initials: L
authors.lastname: Ma
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan, 430073, PR China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan, 430073, PR China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan, 430073, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yang
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan, 430073, PR China.
authors.forename: Donghui
authors.initials: D
authors.lastname: Liang
authors.affiliation: Key Laboratory for Green Chemical Process of Ministry of Education, School of Chemical Engineering and Pharmacy, Wuhan Institute of Technology, Xiongchu Avenue, Wuhan, 430073, PR China. Electronic address: zhonglu@wit.edu.cn.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Lu
mesh_terms: D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D001729:Bismuth / Q000737:chemistry / Q000494:pharmacology; D004357:Drug Synergism; D005839:Gentamicins / Q000737:chemistry* / Q000494:pharmacology*; D006801:Humans; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D054118:Nanospheres / Q000737:chemistry*; D017382:Reactive Oxygen Species; D013211:Staphylococcus aureus / Q000187:drug effects; D013440:Sulfides / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D005839:Gentamicins; D017382:Reactive Oxygen Species; D013440:Sulfides; D001729:Bismuth; C049897:bismuth sulfide
keywords: Bi(2)S(3) nanospheres; Gentamicin; MRSA; Mechanism; Synergistic antibacterial effect