title: Genome-Wide Association Mapping of the Antibody Response to Diphtheria, Tetanus and Acellular Pertussis Vaccine in Mice.
abstract: The objective of the current study was to investigate the genetics of antibody responses to an acellular pertussis vaccine by a genome-wide association study in mice. Female mice of 28 inbred strains received this vaccine at 6, 8, and 12 weeks of age. The antibody titer and avidity of immunoglobulin (Ig) G specific for diphtheria toxin, pertussis toxin, filamentous hemagglutinin and pertactin were measured at 14 and 24 weeks of age. The magnitude, longevity and avidity of IgG differed significantly among mouse strains. There was significant correlation between antigen-specific IgGs for longevity but not for magnitude and avidity. Association mapping and analysis with PolyPhen software identified 6 genetic markers associated with longevity for all 4 antigens, although the expression levels of these genes did not correlate with longevity phenotype. This study provides novel insights into the genetic basis and potential candidate genes for differences in the IgG responses to vaccination.
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine.
authors.forename: Yung-Yi C
authors.initials: YC
authors.lastname: Mosley
authors.affiliation: Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh, Pennsylvania.
authors.forename: Josiah E
authors.initials: JE
authors.lastname: Radder
authors.affiliation: Department of Medicine, Division of Pulmonary, Allergy and Critical Care Medicine, University of Pittsburgh, Pennsylvania.
authors.forename: Annerose
authors.initials: A
authors.lastname: Berndt
authors.affiliation: Department of Comparative Pathobiology, College of Veterinary Medicine.|Purdue Institute of Inflammation, Immunology, and Infectious Diseases, Purdue University, West Lafayette, Indiana.
authors.forename: Harm
authors.initials: H
authors.lastname: HogenEsch
mesh_terms: D000818:Animals; D015721:Diphtheria-Tetanus-Pertussis Vaccine / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D014644:Genetic Variation; D055106:Genome-Wide Association Study; D000071497:Immunogenicity, Vaccine / Q000235:genetics*; D007074:Immunoglobulin G / Q000276:immunology; D008136:Longevity / Q000235:genetics; D051379:Mice; D008815:Mice, Inbred Strains
chemical_list: D015721:Diphtheria-Tetanus-Pertussis Vaccine; D007074:Immunoglobulin G
keywords: DTaP; GWAS; antibody avidity; antibody longevity; antibody titer; genetics; mouse model; pertussis; vaccination