title: Pyrosequencing reveals microbial community dynamics in integrated simultaneous desulfurization and denitrification process at different influent nitrate concentrations.
abstract: Integrated simultaneous desulfurization and denitrification (ISDD) process has proven to be feasible for the coremoval of sulfate, nitrate, and chemical oxygen demand (COD). In this study, we aimed to reveal the microbial community dynamics in the ISDD process with different influent nitrate (NO3-) concentrations. For all tested scenarios, full denitrification was accomplished while sulfate removal efficiency decreased along with increased influent NO3-concentrations. The proportion of S0to influent SO42-maintained a low level (5.6-17.0%) regardless of the increased influent NO3-concentrations. Microbial community analysis results showed that higher influent NO3-concentrations affected the microbial community structure greatly. Phyla Proteobacteria, Spirochaetae, Firmicutes, Synergistetes, and Chloroflexi dominated in all the community compositions, of which Proteobacteria exhibited a clear difference among eight microbial samples. Members of δ-Proteobacteria, with 16S rRNA gene sequences related to Desulfobulbus, were clearly decreased at influent NO3-= 3000 and 3500 mg/L, suggesting an inhibitory effect of NO3-on sulfate reduction. In contrast, as influent NO3-concentration increased, microbial community was notably enriched in γ-Proteobacteria and ε-Proteobacteria, which revealed the enrichment of 16S rRNA gene sequences related to Pseudomonas (γ-Proteobacteria), and Arcobacteria and Sulfurospirillum (ε-Proteobacteria).
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, P.O. Box 2650, 73 Huanghe Road, Nangang District, Harbin, Heilongjiang Province 150090, China.
authors.forename: Chuan
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, P.O. Box 2650, 73 Huanghe Road, Nangang District, Harbin, Heilongjiang Province 150090, China. Electronic address: xuxijun3220@sina.com.
authors.forename: Xi-Jun
authors.initials: XJ
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, P.O. Box 2650, 73 Huanghe Road, Nangang District, Harbin, Heilongjiang Province 150090, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xie
authors.affiliation: School of Environmental Science and Engineering, Yancheng Institute of Technology, Yancheng 224051, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, P.O. Box 2650, 73 Huanghe Road, Nangang District, Harbin, Heilongjiang Province 150090, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, P.O. Box 2650, 73 Huanghe Road, Nangang District, Harbin, Heilongjiang Province 150090, China.
authors.forename: Ai-Jie
authors.initials: AJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, P.O. Box 2650, 73 Huanghe Road, Nangang District, Harbin, Heilongjiang Province 150090, China; Department of Chemical Engineering, National Taiwan University, Taipei 106, Taiwan; Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei 106, Taiwan.
authors.forename: Duu-Jong
authors.initials: DJ
authors.lastname: Lee
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, P.O. Box 2650, 73 Huanghe Road, Nangang District, Harbin, Heilongjiang Province 150090, China. Electronic address: rnq@hit.edu.cn.
authors.forename: Nan-Qi
authors.initials: NQ
authors.lastname: Ren
mesh_terms: D001419:Bacteria / Q000235:genetics / Q000254:growth & development / Q000378:metabolism*; D057919:Biological Oxygen Demand Analysis; D019149:Bioreactors / Q000382:microbiology*; D058440:Denitrification / Q000502:physiology*; D064307:Microbiota / Q000502:physiology*; D009566:Nitrates / Q000737:chemistry / Q000378:metabolism*; D009589:Nitrogen Oxides / Q000378:metabolism; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA / Q000379:methods; D013431:Sulfates / Q000378:metabolism*
chemical_list: D009566:Nitrates; D009589:Nitrogen Oxides; D012336:RNA, Ribosomal, 16S; D013431:Sulfates
keywords: Integrated simultaneous desulfurization and denitrification; Microbial community; Nitrate; Pyrosequencing