title: Comparative "Omics" of the Fusarium fujikuroi Species Complex Highlights Differences in Genetic Potential and Metabolite Synthesis.
abstract: Species of the Fusarium fujikuroi species complex (FFC) cause a wide spectrum of often devastating diseases on diverse agricultural crops, including coffee, fig, mango, maize, rice, and sugarcane. Although species within the FFC are difficult to distinguish by morphology, and their genes often share 90% sequence similarity, they can differ in host plant specificity and life style. FFC species can also produce structurally diverse secondary metabolites (SMs), including the mycotoxins fumonisins, fusarins, fusaric acid, and beauvericin, and the phytohormones gibberellins, auxins, and cytokinins. The spectrum of SMs produced can differ among closely related species, suggesting that SMs might be determinants of host specificity. To date, genomes of only a limited number of FFC species have been sequenced. Here, we provide draft genome sequences of three more members of the FFC: a single isolate of F. mangiferae, the cause of mango malformation, and two isolates of F. proliferatum, one a pathogen of maize and the other an orchid endophyte. We compared these genomes to publicly available genome sequences of three other FFC species. The comparisons revealed species-specific and isolate-specific differences in the composition and expression (in vitro and in planta) of genes involved in SM production including those for phytohormome biosynthesis. Such differences have the potential to impact host specificity and, as in the case of F. proliferatum, the pathogenic versus endophytic life style.
authors.affiliation: Institut für Biologie und Biotechnologie der Pflanzen, Molecular Biology and Biotechnology of Fungi, Westfälische Wilhelms-Universität Münster, Münster, Germany.
authors.forename: Eva-Maria
authors.initials: EM
authors.lastname: Niehaus
authors.affiliation: Institute of Bioinformatics and Systems Biology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Neuherberg, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Münsterkötter
authors.affiliation: United States Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, Illinois.
authors.forename: Robert H
authors.initials: RH
authors.lastname: Proctor
authors.affiliation: United States Department of Agriculture, National Center for Agricultural Utilization Research, Peoria, Illinois.
authors.forename: Daren W
authors.initials: DW
authors.lastname: Brown
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Amir
authors.initials: A
authors.lastname: Sharon
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Yifat
authors.initials: Y
authors.lastname: Idan
authors.affiliation: Department of Molecular Biology and Ecology of Plants, Tel Aviv University, Tel Aviv, Israel.
authors.forename: Liat
authors.initials: L
authors.lastname: Oren-Young
authors.affiliation: Department of Energy Joint Genome Institute, University of California, Walnut Creek, Berkeley, California.
authors.forename: Christian M
authors.initials: CM
authors.lastname: Sieber
authors.affiliation: Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University, Olomouc, Czech Republic.
authors.forename: Ondřej
authors.initials: O
authors.lastname: Novák
authors.affiliation: Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University, Olomouc, Czech Republic.
authors.forename: Aleš
authors.initials: A
authors.lastname: Pěnčík
authors.affiliation: Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University, Olomouc, Czech Republic.
authors.forename: Danuše
authors.initials: D
authors.lastname: Tarkowská
authors.affiliation: Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University, Olomouc, Czech Republic.
authors.forename: Kristýna
authors.initials: K
authors.lastname: Hromadová
authors.affiliation: Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel.
authors.forename: Stanley
authors.initials: S
authors.lastname: Freeman
authors.affiliation: Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel.
authors.forename: Marcel
authors.initials: M
authors.lastname: Maymon
authors.affiliation: Department of Plant Pathology and Weed Research, Agricultural Research Organization (ARO), The Volcani Center, Bet-Dagan, Israel.
authors.forename: Meirav
authors.initials: M
authors.lastname: Elazar
authors.affiliation: Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
authors.forename: Sahar A
authors.initials: SA
authors.lastname: Youssef
authors.affiliation: Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
authors.forename: El Said M
authors.initials: ES
authors.lastname: El-Shabrawy
authors.affiliation: Plant Pathology Research Institute, Agricultural Research Center, Giza, Egypt.
authors.forename: Abdel Baset A
authors.initials: AB
authors.lastname: Shalaby
authors.affiliation: University of Amsterdam, Swammerdam Institute for Life Sciences, Plant Pathology, Amsterdam, The Netherlands.
authors.forename: Petra
authors.initials: P
authors.lastname: Houterman
authors.affiliation: Rheinische Friedrich-Wilhelms-Universität Bonn, Kekulé-Institut für Organische Chemie und Biochemie, Germany.
authors.forename: Nelson L
authors.initials: NL
authors.lastname: Brock
authors.affiliation: Rheinische Friedrich-Wilhelms-Universität Bonn, Kekulé-Institut für Organische Chemie und Biochemie, Germany.
authors.forename: Immo
authors.initials: I
authors.lastname: Burkhardt
authors.affiliation: Department of Microbiology Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.
authors.forename: Elena A
authors.initials: EA
authors.lastname: Tsavkelova
authors.affiliation: Rheinische Friedrich-Wilhelms-Universität Bonn, Kekulé-Institut für Organische Chemie und Biochemie, Germany.
authors.forename: Jeroen S
authors.initials: JS
authors.lastname: Dickschat
authors.affiliation: Centre of the Region Hana for Biotechnological and Agricultural Research, Palacky University, Olomouc, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Galuszka
authors.affiliation: Department of Genome-oriented Bioinformatics, Wissenschaftszentrum Weihenstephan, Technische Universität München, Maximus-von-Imhof-Forum 3, Freising, Germany tudzynsb@uni-muenster.de u.gueldener@tum.de.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Güldener
authors.affiliation: Institut für Biologie und Biotechnologie der Pflanzen, Molecular Biology and Biotechnology of Fungi, Westfälische Wilhelms-Universität Münster, Münster, Germany tudzynsb@uni-muenster.de u.gueldener@tum.de.
authors.forename: Bettina
authors.initials: B
authors.lastname: Tudzynski
mesh_terms: D019143:Evolution, Molecular; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005670:Fusarium / Q000235:genetics* / Q000302:isolation & purification / Q000472:pathogenicity; D016681:Genome, Fungal; D058507:Host Specificity / Q000235:genetics*; D031022:Mangifera / Q000382:microbiology; D055442:Metabolome; D029595:Orchidaceae / Q000382:microbiology; D011110:Polymorphism, Genetic; D003313:Zea mays / Q000382:microbiology
chemical_list: D005656:Fungal Proteins
keywords: Fusarium fujikuroi species complex; evolution; genome sequencing; in planta expression; metabolomics; secondary metabolism