title: Rehmannia glutinosapolysaccharide liposome as a novel strategy for stimulating an efficient immune response and their effects on dendritic cells.
abstract: Nanomedicine, the medical application of nanotechnology, promises a seemingly limitless range of applications from drug delivery to adjuvants and therapeutics. Our current research is focused on natural polymer-based liposome adjuvants. With the aim of inducing protective and long-lasting immunity, the immunological adjuvant activity ofRehmannia glutinosapolysaccharide liposome (RGPL) was investigated. In vivo, the splenic lymphocyte proliferation ratios and ovalbumin-specific immunoglobulin G titers of ovalbumin-RGPL-vaccinated mice were significantly upregulated. In draining lymph nodes, the expression of MHC II+CD11c+and CD86+CD11c+was increased by RGPL; in addition, the percentages of central memory cells (TCM) and effector memory cells (TEM) were also elevated. RGPL could effectively provide adequate antigen exposure in lymph nodes. In vitro, RGPL could promote dendritic cell maturation and enhance dendritic cell functions, such as the mixed lymphocyte reaction and antigen presentation. Overall, the results demonstrated that RGPL has the potential to act as an effective controlled release vaccine adjuvant.
authors.affiliation: College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing.
authors.forename: Yee
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Veterinary Medicine, Fujian Agriculture and Forestry University, Fuzhou, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Qin
authors.affiliation: College of Animal Science and Veterinary Medicine, Fujian Agriculture and Forestry University, Fuzhou, People's Republic of China.
authors.forename: Yifan
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing.
authors.forename: Zhenguang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing.
authors.forename: Ruonan
authors.initials: R
authors.lastname: Bo
authors.affiliation: College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing.
authors.forename: Yuanliang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing.
authors.forename: Jiaguo
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing.
authors.forename: Yi
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Veterinary Medicine, Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing; College of Animal Science and Veterinary Medicine, Fujian Agriculture and Forestry University, Fuzhou, People's Republic of China.
authors.forename: Deyun
authors.initials: D
authors.lastname: Wang
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage*; D000818:Animals; D017951:Antigen Presentation / Q000187:drug effects*; D051940:B7-2 Antigen / Q000737:chemistry; D039521:CD11c Antigen / Q000737:chemistry; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016207:Cytokines / Q000737:chemistry; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D005260:Female; D007074:Immunoglobulin G / Q000737:chemistry; D007150:Immunohistochemistry; D007156:Immunologic Memory; D008081:Liposomes / Q000494:pharmacology*; D008198:Lymph Nodes / Q000187:drug effects; D008214:Lymphocytes / Q000187:drug effects; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D011134:Polysaccharides / Q000494:pharmacology*; D032267:Rehmannia / Q000737:chemistry*; D013154:Spleen / Q000187:drug effects; D013601:T-Lymphocytes / Q000166:cytology
chemical_list: D000276:Adjuvants, Immunologic; D051940:B7-2 Antigen; D039521:CD11c Antigen; D016207:Cytokines; D007074:Immunoglobulin G; D008081:Liposomes; D011134:Polysaccharides; D010047:Ovalbumin
keywords: Rehmannia glutinosa polysaccharide liposome; adjuvant; controlled release; dendritic cells; ovalbumin