title: Characterization and application of newly developed polymorphic microsatellite markers in the Ezo red fox (Vulpes vulpes schrencki).
abstract: The Ezo red fox (Vulpes vulpes schrencki), a subspecies endemic to Hokkaido island, Japan, is a known host species for the tapeworm Echinococcus multilocularis. To develop tools for molecular ecological studies, we isolated 28 microsatellite regions from the genome of Ezo red fox, and developed 18 polymorphic microsatellite markers. These markers were characterized using 7 individuals and 22 fecal samples of the Ezo red fox. The number of alleles for these markers ranged from 1 to 7, and the observed heterozygosity, estimated on the basis of the genotypes of 7 individuals, ranged from 0.29 to 1.00. All markers, except DvNok5, were in Hardy-Weinberg equilibrium (P > 0.05), and no linkage disequilibrium was detected among these loci, except between DvNok14 and DvNok28 (P = 0.01). Moreover, six microsatellite loci were successfully genotyped using feces-derived DNA from the Ezo red fox. The markers developed in our study might serve as a useful tool for molecular ecological studies of the Ezo red fox.
authors.affiliation: Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.
authors.forename: T
authors.initials: T
authors.lastname: Tada
authors.affiliation: Mammalian Genetics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Seki
authors.affiliation: Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kameyama
authors.affiliation: Mammalian Genetics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan.
authors.forename: Y
authors.initials: Y
authors.lastname: Kikkawa
authors.affiliation: Graduate School of Bioindustry, Tokyo University of Agriculture, Abashiri, Hokkaido, Japan k3wada@bioindustry.nodai.ac.jp.|Mammalian Genetics Project, Department of Genome Medicine, Tokyo Metropolitan Institute of Medical Science, Tokyo, Japan k3wada@bioindustry.nodai.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Wada
mesh_terms: D000818:Animals; D005243:Feces / Q000737:chemistry; D005589:Foxes / Q000235:genetics*; D005819:Genetic Markers / Q000235:genetics; D060005:Genotyping Techniques / Q000379:methods*; D006579:Heterozygote; D018895:Microsatellite Repeats
chemical_list: D005819:Genetic Markers