title: Temporal and spatial expression pattern of Nnat during mouse eye development.
abstract: Neuronatin (Nnat) was initially identified as a highly expressed gene in neonatal mammalian brain. In this study, we analyze the spatial and temporal expression pattern of Nnat during mouse eye development as well as in the adult.
  
  
  
  The expression of Nnat was analyzed on mRNA as well as protein level. The presence of Nnat transcripts in the adult retina was examined using reverse transcription-polymerase chain reaction (RT-PCR). Nnat protein expression was evaluated by Western blot and immunohistochemistry during eye development at embryonic day (E) 12, 15, 16 and postnatal day (P) 7, 14, 30 and 175 (adult).
  
  
  
  Immunohistochemical studies of the developing mouse eye revealed Nnat expression in embryonic and adult neuroretina as well as in corneal epithelial, stromal, endothelial cells and in lens epithelium. Expression of Nnat was detected from E12 onwards and was also present in adult eyes.
  
  
  
  The expression pattern suggests that Nnat may play an important role during eye development and in the maintenance of mature eye.
authors.affiliation: Department of Ophthalmology, University Heidelberg, Germany. Electronic address: saadettinsel@googlemail.com.
authors.forename: Saadettin
authors.initials: S
authors.lastname: Sel
authors.affiliation: Department of Ophthalmology, University Heidelberg, Germany.
authors.forename: Eva
authors.initials: E
authors.lastname: Patzel
authors.affiliation: Department of Ophthalmology, University Heidelberg, Germany.
authors.forename: Lucia
authors.initials: L
authors.lastname: Poggi
authors.affiliation: Department of Ophthalmology, University Heidelberg, Germany.
authors.forename: Delia
authors.initials: D
authors.lastname: Kaiser
authors.affiliation: Institute of Pathology, University Magdeburg, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kalinski
authors.affiliation: Institut for Anatomy II, University Erlangen, Germany.
authors.forename: Martin
authors.initials: M
authors.lastname: Schicht
authors.affiliation: Institut for Anatomy II, University Erlangen, Germany.
authors.forename: Friedrich
authors.initials: F
authors.lastname: Paulsen
authors.affiliation: Institute of Pathology, University Magdeburg, Germany.
authors.forename: Norbert
authors.initials: N
authors.lastname: Nass
mesh_terms: D000818:Animals; D005123:Eye / Q000166:cytology / Q000254:growth & development* / Q000378:metabolism*; D005260:Female; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008565:Membrane Proteins / Q000235:genetics* / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D009419:Nerve Tissue Proteins / Q000235:genetics* / Q000378:metabolism; D012160:Retina / Q000378:metabolism
chemical_list: D008565:Membrane Proteins; D009419:Nerve Tissue Proteins; C490239:Nnat protein, mouse
keywords: Development; Eye; Mouse; Neuronatin; Nnat