title: Biomimetic apatite formed on cobalt-chromium alloy: A polymer-free carrier for drug eluting stent.
abstract: In this study, sirolimus (SRL) was loaded within biomimetic apatite formed on cobalt-chromium (Co-Cr) alloy, which has been reported for the first time, to inhibit the in-stent restenosis. Two different groups of loading SRL within biomimetic apatite were prepared: Group A (mono-layer of apatite/SRL) and Group B (bi-layer of apatite/SRL). Group A and Group B showed the biphasic pattern of SRL release up to 40 and 90days, respectively. The attachment of human artery smooth muscle cell (HASMC) for both Group A and Group B was significantly inhibited, and proliferation dramatically decreased with the release of SRL. Noteworthily, biomimetic apatite alone also suppressed the SMC proliferation. The porous biomimetic apatite uniformly covered Co-Cr stent without crack or webbings. After balloon expansion, the integrity of biomimetic apatite was sufficient to resist delamination or destruction. Thus, this study demonstrated that biomimetic apatite is a promising drug carrier for potential use in stents.
authors.affiliation: Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China; Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, PR China.
authors.forename: Cen
authors.initials: C
authors.lastname: Chen
authors.affiliation: Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.
authors.forename: Chenxue
authors.initials: C
authors.lastname: Yao
authors.affiliation: Materials Science and Engineering, College of Mechanical and Electrical Engineering, Beijing Union University, Beijing, 100020, PR China.
authors.forename: Jingxin
authors.initials: J
authors.lastname: Yang
authors.affiliation: Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Luo
authors.affiliation: Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Kong
authors.affiliation: Biomaterials R&D Center, GENOSS Co., Ltd., Suwon-si, 443-270, South Korea.
authors.forename: Sung-Min
authors.initials: SM
authors.lastname: Chung
authors.affiliation: Bio-X Center, College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, PR China; Institute of Natural Science, Yonsei University, Seoul, 03722, South Korea. Electronic address: inseop@yonsei.ac.kr.
authors.forename: In-Seop
authors.initials: IS
authors.lastname: Lee
mesh_terms: D001031:Apatites / Q000737:chemistry; D001158:Arteries / Q000166:cytology; D040761:Biomimetic Materials / Q000737:chemistry*; D002448:Cell Adhesion; D049109:Cell Proliferation; D002858:Chromium Alloys / Q000737:chemistry*; D003035:Cobalt / Q000737:chemistry*; D023903:Coronary Restenosis; D016503:Drug Delivery Systems; D054855:Drug-Eluting Stents; D006801:Humans; D032389:Myocytes, Smooth Muscle / Q000166:cytology; D011108:Polymers / Q000737:chemistry*; D016062:Porosity; D020123:Sirolimus / Q000008:administration & dosage; D013499:Surface Properties; D014961:X-Ray Diffraction
chemical_list: D001031:Apatites; D002858:Chromium Alloys; D011108:Polymers; D003035:Cobalt; D020123:Sirolimus
keywords: Biomimetic apatite; Drug-eluting stent; Polymer-free; Sirolimus; Smooth muscle cell