title: The association between interleukin-10 (IL-10) -592C/A, -819T/C, -1082G/A promoter polymorphisms and endometriosis.
abstract: OBJECTIVE
  Endometriosis has an incidence reaching up to 50% in infertile women. Cytokine-mediated immune responses seem to play an important role in endometriosis pathogenesis, but still the etiology and pathophysiology remain unclear. In the current study we tried to investigate whether there is a relationship between IL-10 genetic polymorphism, serum levels of IL-10 and the presence of advanced endometriosis.
  
  
  METHODS
  The presence of IL-10 592C/A, 819T/C, 1082G/A promoter polymorphisms and IL-10 serum levels were investigated in advanced endometriosis patients compared to healthy controls. Genomic DNA was extracted from peripheral blood leukocytes and further analyzed by PCR.
  
  
  RESULTS
  IL-10 serum levels were higher in endometriosis group compared to controls (1.48, 0.68, p < 0.001). We have observed an association between IL-10 592C/C and 819C/C genotypes, presence of C alleles and an increased risk of endometriosis. No difference was observed in IL-10 serum levels corresponding to different alleles or genotypes.
  
  
  CONCLUSIONS
  Our results suggest that IL-10 592A/C and 819T/C promoter polymorphisms confer susceptibility to advanced endometriosis. No associations were found between the IL-10 1082A/G polymorphism and susceptibility to advanced endometriosis.
authors.affiliation: Second Obstetrics and Gynecology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur st, 400012, Cluj-Napoca, Cluj, Romania.
authors.forename: Andrei Mihai
authors.initials: AM
authors.lastname: Malutan
authors.affiliation: Medical Biochemistry Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur st, 400012, Cluj-Napoca, Cluj, Romania. cdrugan@umfcluj.ro.
authors.forename: Cristina
authors.identifier: http://orcid.org/0000-0001-9136-2530
authors.initials: C
authors.lastname: Drugan
authors.affiliation: Department of Obstetrics and Gynecology, Medical University of Vienna, Waehringer Guertel 18-20, 1090, Vienna, Austria.
authors.forename: Katharina
authors.initials: K
authors.lastname: Walch
authors.affiliation: Medical Informatics and Biostatistics Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur st, 400012, Cluj-Napoca, Cluj, Romania.
authors.forename: Tudor
authors.initials: T
authors.lastname: Drugan
authors.affiliation: Second Obstetrics and Gynecology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur st, 400012, Cluj-Napoca, Cluj, Romania.
authors.forename: Razvan
authors.initials: R
authors.lastname: Ciortea
authors.affiliation: Second Obstetrics and Gynecology Department, "Iuliu Hatieganu" University of Medicine and Pharmacy, 6 Louis Pasteur st, 400012, Cluj-Napoca, Cluj, Romania.
authors.forename: Dan
authors.initials: D
authors.lastname: Mihu
mesh_terms: D000328:Adult; D016022:Case-Control Studies; D004715:Endometriosis / Q000209:etiology / Q000235:genetics*; D005260:Female; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D016753:Interleukin-10 / Q000097:blood / Q000235:genetics*; D008875:Middle Aged; D011110:Polymorphism, Genetic; D011401:Promoter Regions, Genetic
chemical_list: C508609:IL10 protein, human; D016753:Interleukin-10
keywords: Cytokine; Endometriosis; Genetic polymorphism; Interleukin-10