title: [GenomeLabTMGeXP analysis system to detect apoptosis-associated genes in A549 cells treated by recombinant human IL-24 combined with cisplatin].
abstract: Objective To investigate the changes of apoptosis-associated genes in human lung adenocarcinoma cell line A549 after treated by the recombinant human interleukin-24 (rhIL-24) combined with cisplatin (DDP). Methods The mRNA levels of Bax, Bcl-2, survivin, caspase-3, retinoblastoma (Rb) gene, p53 were analyzed by GenomeLabTMGeXP genetic analysis system after A549 cells were treated with 160 ng/mL rhIL-24 or 3 μg/mL DDP or 160 ng/mL rhIL-24 combined with 3 μg/mL DDP. Results The rhIL-24 up-regulated the levels of Bax, Rb, caspase-3 mRNAs and down-regulated Bcl-2, survivin mRNAs, but the change of p53 mRNA was not regular. The combination of rhIL-24 and DDP strengthened the above changes. Conclusion The rhIL-24 protein can induce the apoptosis of A549 cells by up-regulating Bax, caspase-3, Rb mRNAs and down-regulating Bcl-2, survivin mRNAs.
authors.affiliation: Zhuhai Campus of Zunyi Medical College, Immunological Graduate Education Innovation Base of Guizhou Province, Zhuhai 519041, China.
authors.forename: Jinjin
authors.initials: J
authors.lastname: Guo
authors.affiliation: Zhuhai Campus of Zunyi Medical College, Immunological Graduate Education Innovation Base of Guizhou Province, Zhuhai 519041, China.
authors.forename: Shaohui
authors.initials: S
authors.lastname: Wang
authors.affiliation: Zhuhai Campus of Zunyi Medical College, Immunological Graduate Education Innovation Base of Guizhou Province, Zhuhai 519041, China. *Corresponding author, E-mail: sunwb7224@sina.com.
authors.forename: Wanbang
authors.initials: W
authors.lastname: Sun
authors.affiliation: Zhuhai Campus of Zunyi Medical College, Immunological Graduate Education Innovation Base of Guizhou Province, Zhuhai 519041, China.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Tang
authors.affiliation: Zhuhai Campus of Zunyi Medical College, Immunological Graduate Education Innovation Base of Guizhou Province, Zhuhai 519041, China.
authors.forename: Guangbin
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Zhuhai Campus of Zunyi Medical College, Immunological Graduate Education Innovation Base of Guizhou Province, Zhuhai 519041, China.
authors.forename: Mingying
authors.initials: M
authors.lastname: Song
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis / Q000187:drug effects* / Q000235:genetics*; D051017:Apoptosis Regulatory Proteins / Q000235:genetics*; D045744:Cell Line, Tumor; D002945:Cisplatin / Q000008:administration & dosage*; D006801:Humans; D007378:Interleukins / Q000008:administration & dosage*; D011994:Recombinant Proteins / Q000008:administration & dosage*; D000099180:Interleukin-24
chemical_list: D051017:Apoptosis Regulatory Proteins; D002945:Cisplatin; D007378:Interleukins; D011994:Recombinant Proteins; D000099180:Interleukin-24