title: Evaluation of Immunochromatographic Tests and a New Enzyme Immunoassay for Detection of a Novel GII.17 Norovirus in Stool Samples.
abstract: A novel GII.17 norovirus (NoV), Kawasaki 2014, has spread to several regions of the world. Rapid and reliable diagnostic tests are needed for the detection of this new NoV variant. In this study,analytical sensitivity of 7 different immunochromatographic (IC) test kits (6 are on the market in Japan and one in Europe) was evaluated by means of confirmed GII.17 NoV-positive stool samples. The stool samples were also tested by a bioluminescent enzyme immunoassay (BLEIA). Real-time RT-PCR served as a reference (gold standard) method. Among the 7 IC kits, RIDA QUICK was the most sensitive, with the limit of detection of 107copies/ml, whereas the limits of detection of the other IC kits ranged from 108to 109copies/ml. It should be pointed out that the limit of detection of BLEIA was approximately 100- to 1,000-fold better (104-105copies/ml) than that of RIDA QUICK. Nevertheless, the procedure of BLEIA took more time and required sophisticated equipment.
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.|Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Ushijima
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.|Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo.
authors.forename: Aksara
authors.initials: A
authors.lastname: Thongprachum
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University.
authors.forename: Pattara
authors.initials: P
authors.lastname: Khamrin
authors.affiliation: Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo.
authors.forename: Sayaka
authors.initials: S
authors.lastname: Takanashi
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.|Department of Developmental Medical Sciences, School of International Health, Graduate School of Medicine, The University of Tokyo.
authors.forename: Shoko
authors.initials: S
authors.lastname: Okitsu
authors.affiliation: Department of Microbiology, Faculty of Medicine, Chiang Mai University.
authors.forename: Niwat
authors.initials: N
authors.lastname: Maneekarn
authors.affiliation: Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Hayakawa
mesh_terms: D000328:Adult; D017250:Caliciviridae Infections / Q000175:diagnosis*; D002648:Child; D002846:Chromatography, Affinity / Q000379:methods*; D019411:Clinical Laboratory Techniques / Q000379:methods*; D005243:Feces / Q000821:virology*; D006801:Humans; D007124:Immunoenzyme Techniques / Q000379:methods*; D029322:Norovirus / Q000276:immunology / Q000302:isolation & purification*; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity
keywords: Bioluminescent enzyme immunoassay; GII.17; Immunochromatography; Norovirus; gastroenteritis