title: Plasma Phospholipid Fatty Acid Profile is Altered in Both Septic and Non-Septic Critically Ill: A Correlation with Inflammatory Markers and Albumin.
abstract: This study analyzes fatty acid (FA) composition in plasma lipids and erythrocyte phospholipids while comparing septic and non-septic critically ill patients. The aim was to describe impacts of infection and the inflammatory process. Patients with severe sepsis (SP, n = 13); age-, sex- and APACHE II score-matched non-septic critically ill with systemic inflammatory response syndrome (NSP, n = 13); and age-/sex-matched healthy controls (HC, n = 13) were included in a prospective case-control study during the first 24 h after admission to the intensive care unit. In both SP and NSP, lower n-6 polyunsaturated FA (PUFA) accompanied by higher proportions of monounsaturated FA (MUFA) in plasma phospholipids (PPL) was observed relative to HC. MUFA proportion was negatively correlated with n-6 PUFA, high density lipoprotein cholesterol (HDL-C), and albumin. MUFA was positively correlated with C-reactive protein (CRP), procalcitonin (PCT), interleukins (IL-6, IL-10), oxidized low density lipoproteins (ox-LDL), and conjugated dienes (CD). In both SP and NSP, inflammatory and lipid peroxidation markers were significantly higher-CRP (p < 0.001; p = 0.08), IL-6, IL-10, TNF-α (p < 0.01, p = 0.06), ox-LDL, and CD while total cholesterol, HDL-C, LDL-C albumin, and 20:4n-6/22:6n-3 and n-6/n-3 ratios were lower compared to HC. In conclusion, the changes in plasma lipid FA profile relate to the intensity of inflammatory and peroxidative response regardless of insult etiology. The lower MUFA and higher n-6 PUFA proportions in PPL were inversely correlated with cholesterol and albumin levels.
authors.affiliation: 4th Department of Internal Medicine, 1st Faculty of Medicine, General University Hospital, Charles University, U Nemocnice 2, 128 08, Prague, Czech Republic. fnova@lf1.cuni.cz.
authors.forename: František
authors.initials: F
authors.lastname: Novak
authors.affiliation: 4th Department of Internal Medicine, 1st Faculty of Medicine, General University Hospital, Charles University, U Nemocnice 2, 128 08, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Borovska
authors.affiliation: 4th Department of Internal Medicine, 1st Faculty of Medicine, General University Hospital, Charles University, U Nemocnice 2, 128 08, Prague, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Vecka
authors.affiliation: 4th Department of Internal Medicine, 1st Faculty of Medicine, General University Hospital, Charles University, U Nemocnice 2, 128 08, Prague, Czech Republic.
authors.forename: J
authors.initials: J
authors.lastname: Rychlikova
authors.affiliation: 4th Department of Internal Medicine, 1st Faculty of Medicine, General University Hospital, Charles University, U Nemocnice 2, 128 08, Prague, Czech Republic.
authors.forename: L
authors.initials: L
authors.lastname: Vavrova
authors.affiliation: 4th Department of Internal Medicine, 1st Faculty of Medicine, General University Hospital, Charles University, U Nemocnice 2, 128 08, Prague, Czech Republic.
authors.forename: H
authors.initials: H
authors.lastname: Petraskova
authors.affiliation: 4th Department of Internal Medicine, 1st Faculty of Medicine, General University Hospital, Charles University, U Nemocnice 2, 128 08, Prague, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Zak
authors.affiliation: Department of Physiology, Faculty of Science, Charles University, Viničná 7, 128 44, Prague, Czech Republic.|Institute of Physiology, Czech Academy of Sciences, Vídeňská 1083, 142 20, Prague, Czech Republic.
authors.forename: O
authors.initials: O
authors.lastname: Novakova
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood*; D002097:C-Reactive Protein / Q000378:metabolism; D002116:Calcitonin / Q000378:metabolism; D016022:Case-Control Studies; D016638:Critical Illness; D005227:Fatty Acids / Q000097:blood*; D005229:Fatty Acids, Monounsaturated / Q000097:blood; D005260:Female; D006801:Humans; D007378:Interleukins / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D010743:Phospholipids / Q000097:blood*; D011446:Prospective Studies; D018805:Sepsis / Q000276:immunology* / Q000378:metabolism; D018746:Systemic Inflammatory Response Syndrome / Q000276:immunology*
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D005229:Fatty Acids, Monounsaturated; D007378:Interleukins; D010743:Phospholipids; D002116:Calcitonin; D002097:C-Reactive Protein
keywords: Fatty acid profile; Inflammation; Lipoproteins; Oxidative stress; PUFA; Plasma lipids; Sepsis