title: Diastase induced green synthesis of bilayered reduced graphene oxide and its decoration with gold nanoparticles.
abstract: In this paper, we report an enzyme dependent, green one-pot deoxygenation cum decoration method to synthesize diastase-conjugated reduced graphene oxide (DRG) nanosheets, DRG/gold nanoparticles (DRG/Au) composite. The DRG synthesis was completed in 7h under heating at 90°C on water bath. Selected area electron diffraction (SAED) and Atomic force microscopy (AFM) study has revealed the formation of bilayered reduced graphene oxide sheets. Transmission electron microscopy (TEM) images of DRG/Au composite have shown the uniform decoration of gold nanoparticles (AuNPs) onto the DRG nanosheet surface. Fourier transform infrared spectroscopy (FTIR) and Raman results additionally have shown the functionalization of enzyme molecules onto the DRG nanosheet surface after reduction making it as an effective platform towards the efficient binding of gold nanoparticles. In vitro cytotoxicity studies by MTT assay on A549 and HCT116 cell lines exhibited that the cytotoxicity of the prepared graphene oxide (GO), DRG and DRG/Au is dose dependant. These results have shown that this synthetic method is effective for the production of large scale graphene in a low cost, simple and green method. Since this process avoids the use of hazardous and toxic substances, the produced DRG/Au composites are likely to offer various potential applications in biology and medicine.
authors.affiliation: The Key laboratory of Advanced Textile Materials and Manufacturing Technology of Ministry of Education, National Engineering Lab for Textile Fiber Materials and Processing Technology (Zhejiang), College of Materials and Textiles, Zhejiang Sci-Tech University, Hangzhou 310018, China; Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Sireesh Babu
authors.initials: SB
authors.lastname: Maddinedi
authors.affiliation: Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India. Electronic address: badalkmandal@hotmail.com.
authors.forename: Badal Kumar
authors.initials: BK
authors.lastname: Mandal
authors.affiliation: Center for Material Characterization (CMC), National Chemical Laboratory, Pune 411008, India.
authors.forename: Sagar Hindurao
authors.initials: SH
authors.lastname: Patil
authors.affiliation: Chemistry Technology Centre of Catalonia, Universitat Rovira i Virgili, Tarragona, Spain.
authors.forename: Vaibhav Vilas
authors.initials: VV
authors.lastname: Andhalkar
authors.affiliation: Nano-food Research Group, Instrumental and Food Analysis Laboratory, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors.forename: Shivendu
authors.initials: S
authors.lastname: Ranjan
authors.affiliation: Nano-food Research Group, Instrumental and Food Analysis Laboratory, School of Bio Sciences and Technology, VIT University, Vellore, Tamil Nadu, India.
authors.forename: Nandita
authors.initials: N
authors.lastname: Dasgupta
mesh_terms: D000681:Amylases / Q000737:chemistry*; D006046:Gold / Q000737:chemistry*; D006108:Graphite / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D046529:Microscopy, Electron, Transmission; D010087:Oxides / Q000737:chemistry; D013057:Spectrum Analysis / Q000379:methods
chemical_list: D010087:Oxides; D006046:Gold; D006108:Graphite; D000681:Amylases
keywords: Diastase; Reduced graphene oxide; Stabilizing agent