title: The dielectrophoretic disassociation of chloride ions and the influence on diamagnetic anisotropy in cell membranes.
abstract: Chloride channels represent ubiquitously expressed proteins that regulate fundamental cellular processes including membrane potential, maintenance of intracellular pH, and regulation of cell volume. However, mechanisms to modulate this large family of ion channels have remained elusive to date. This large chloride channel family does not appear to operate with selectivity similar to the sodium and potassium channels. These unique channels appear to be bi-directional cotransporters of two or more different molecules or ions across a bilayer phospholipid membrane. Here we show how 3 amperes of direct current from a device that generates an electromagnetic field in a 3 mM hypotonic saline solution leads to a dielectrophoretic disassociation of the chloride ion from its chloro-metabolites transforming it into a polymorphic diamagnetically disassociated bio-chloride (bCl-). This field treated aqueous solution appears to continue to induce a magnetic moment change in solution for some hours when no longer under the influence of the direct current; for when this field influenced solution is used to reconstitute growth media of human breast carcinoma (MDA-MB-231) and human breast epithelial (MCF-10A) cells in vitro, significant changes in chloride ion channel expression, membrane potential, cell volume, and a massive transcriptional reprogramming of 2,468 genes expressions by Human Genome U133 Plus 2.0 Gene Chip Array (Affymetrix) analyses occur. We will highlight how the strong changes in chloride ion channel expression and cell physiology could be intricately linked to enhanced diamagnetic anisotropy in cell membranes that occur under the influence of this disassociated polymorphic bCl-.
authors.affiliation: Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN 38163, USA.|The Loewenberg College of Nursing, University of Memphis, Memphis, TN 38152, USA.
authors.forename: Marcy C
authors.initials: MC
authors.lastname: Purnell
authors.affiliation: The Loewenberg College of Nursing, University of Memphis, Memphis, TN 38152, USA.|Electroceutics, LLC, Hernando, MS 38652, USA.
authors.forename: Terence J
authors.initials: TJ
authors.lastname: Skrinjar
mesh_terms: D016880:Anisotropy; D002460:Cell Line; D045744:Cell Line, Tumor; D002462:Cell Membrane / Q000378:metabolism*; D018118:Chloride Channels / Q000378:metabolism*; D006801:Humans; D008564:Membrane Potentials / Q000235:genetics / Q000502:physiology; D020133:Reverse Transcriptase Polymerase Chain Reaction
chemical_list: D018118:Chloride Channels