title: Characterization of microbial population of breba and main crops (Ficus carica) during cold storage: Influence of passive modified atmospheres (MAP) and antimicrobial extract application.
abstract: The purpose of this work was to study the changes of bacterial and fungal population of breba fruits such as 'Banane' and 'San Antonio' as well as 'Cuello Dama Negro', 'Cuello Dama Blanco' and 'San Antonio' fig cultivars stored in passive modified atmospheres (MAP) by the use of three different microperforated films (M10 with 16 holes; M30 with five holes and M50 with three holes). Moreover the effects of the application of aqueous soy polyphenolic antimicrobial extract (APE), alone or combined with MAP, were also studied for 'Cuello Dama Negro' and 'Cuello Dama Blanco' fig cultivars. Bacteria and fungi isolates were identified by PCR-RFLP of 16S rRNA and ITS regions, respectively, and subsequently sequence of the different patterns obtained. The results indicated that Pseudomonas gessardii, Pantoea agglomerans and Enterobacter asburiae were the main species of bacteria found in all the treatments studied. The fungal species identified were Aureobasidium pulullans, Cladosporium cladosporioides and Alternaria alternata, which were found in a lower percentage in fruit stored in MAP and fruits treated with antimicrobial extracts, as this treatments allowed to reduce the microbial growth of moulds and yeasts. Thus, the application of treatments such as M30, M50 or the combination of MAP with antimicrobial extract was highly effective to control fruit spoilage in fig and breba crops.
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avda. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Investigación en Recursos Agrarios (INURA), Avda. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: María Del Carmen
authors.initials: MDC
authors.lastname: Villalobos
authors.affiliation: Instituto Tecnológico Agroalimentario de Extremadura (INTAEX-CICYTEX), Área de Vegetales, Junta de Extremadura, Avda, Adolfo Suárez s/n, 06007 Badajoz, Spain.
authors.forename: Manuel Joaquín
authors.initials: MJ
authors.lastname: Serradilla
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avda. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Investigación en Recursos Agrarios (INURA), Avda. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Martín
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avda. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Investigación en Recursos Agrarios (INURA), Avda. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Hernández-León
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avda. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Investigación en Recursos Agrarios (INURA), Avda. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Ruíz-Moyano
authors.affiliation: Nutrición y Bromatología, Escuela de Ingenierías Agrarias, Universidad de Extremadura, Avda. Adolfo Suárez s/n, 06007 Badajoz, Spain; Instituto Universitario de Investigación en Recursos Agrarios (INURA), Avda. de la Investigación s/n, Campus Universitario, 06006 Badajoz, Spain. Electronic address: mdeguia@unex.es.
authors.forename: María de Guía
authors.initials: MG
authors.lastname: Córdoba
mesh_terms: D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D001274:Atmospheric Pressure; D001419:Bacteria / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D030681:Ficus / Q000382:microbiology*; D005516:Food Microbiology / Q000379:methods; D018857:Food Packaging; D005519:Food Preservation / Q000379:methods*; D061353:Food Storage; D005658:Fungi / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D059013:Microbial Consortia / Q000187:drug effects / Q000235:genetics; D011549:Pseudomonas / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D013025:Glycine max / Q000737:chemistry
chemical_list: D000890:Anti-Infective Agents
keywords: Antimicrobial extracts; Fig; Microbial population; Modified atmospheres