title: Polyethyleneimine modified poly(Hyaluronic acid) particles with controllable antimicrobial and anticancer effects.
abstract: Poly(hyaluronic acid) (p(HA)) particles with sizes from few hundred nm to few tens of micrometer were synthesized by using epoxy groups containing crosslinker glycerol diglycidyl ether (GDE) with high yield, 94±5%. P(HA) particles were oxidized by treatment with sodium periodate and then reacted with cationic polyethyleneimine (PEI) at 1:0.5, 1:1, and 1:2 wt ratio of p(HA):PEI to obtain p(HA)-PEI particles. From zeta potential measurements, isoelectronic points of bare p(HA) particles increased to pH 8.7 from 2.7 after modification with cationic PEI. New properties, such as antibacterial property, were attained for p(HA)-PEI after modification. The highest minimum bactericidal concentration (MBC) values were 0.5, 1, and 0.5mg/mL against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis species for 1:0.5 ratio of p(HA)-PEI at 72h incubation time. Moreover, the p(HA)-PEI particles were found to be biocompatible with L929 fibroblast cells, and interestingly, p(HA)-PEI particles were found to inhibit MDA-MB-231 breast and H1299 cancer cell growth depending on amount of PEI in p(HA)-PEI particles.
authors.affiliation: Faculty of Science & Arts, Chemistry Department, 17100 Canakkale, Turkey; Nanoscience and Technology Research and Application Center (NANORAC), Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey. Electronic address: sahiner71@gmail.com.
authors.forename: Nurettin
authors.initials: N
authors.lastname: Sahiner
authors.affiliation: Faculty of Science & Arts, Chemistry Department, 17100 Canakkale, Turkey.
authors.forename: Selin
authors.initials: S
authors.lastname: Sagbas
authors.affiliation: Department of Leather Engineering, Ege University, Bornova, 35100, Izmir, Turkey.
authors.forename: Mehtap
authors.initials: M
authors.lastname: Sahiner
authors.affiliation: Department of Ophthalmology, School of Medicine, Tulane University, 70112, New Orleans, LA, United States.
authors.forename: Ramesh S
authors.initials: RS
authors.lastname: Ayyala
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000890:Anti-Infective Agents / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects*; D002460:Cell Line; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D005347:Fibroblasts / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology*; D051379:Mice; D011094:Polyethyleneimine / Q000737:chemistry* / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D011094:Polyethyleneimine; D006820:Hyaluronic Acid
keywords: Antimicrobial HA particles; Biocompatible p(HA)-PEI particles; Cancer treatment; Hyaluronic acid microgel/nanogel; PEI-modified HA particles