title: Assessment of the Number and Phenotype of Macrophages in the Human BMB Samples of CML.
abstract: Macrophages have emerged as a key player in tumor biology. However, their number and phenotype in human bone marrow of biopsy (BMB) samples of chronic myeloid leukemia (CML) and their association with disease progression from an initial chronic phase (CP) to accelerated phase (AP) to advanced blast phase (BP) are still unclear. BMB samples from 127 CML patients and 30 patients with iron-deficiency anemia (IDA) as control group were analyzed by immunohistochemistry. The expression levels of CD68, CD163, and CD206 in BMB samples of CML patients were significantly higher than those in the patients of control group (P< 0.01), and we observed that their positive expression was gradually elevated during the transformation of CML-CP to AP to BP (P< 0.01). However, the expressions of CD68, CD163, and CD206 in released group were downregulated and contrasted to these in control group; there exists statistical significance (P< 0.01). The percentage ratio of CD163 and CD206 to CD68 was pronounced to be increasing from CML-CP to AP to BP (P< 0.01). Hence, the higher proportion of CD68+, CD163+and CD206+macrophages in BMB samples can be considered a key factor for disease progression of CML patients. Targeting macrophages, especially the M2 phenotype may help in designing therapeutic strategies for CML.
authors.affiliation: Department of Clinical Laboratory, Yunnan Provincial First People's Hospital, Kunming, Yunnan 650032, China; Institute of Clinical and Basic Medical Sciences, Yunnan Provincial First People's Hospital, Kunming, Yunnan 650032, China.
authors.forename: Jian-Xin
authors.identifier: 0000-0002-7166-3982
authors.initials: JX
authors.lastname: Song
authors.affiliation: Department of Clinical Laboratory, Yunnan Provincial First People's Hospital, Kunming, Yunnan 650032, China.
authors.forename: Zi-Jin
authors.identifier: 0000-0002-4337-2520
authors.initials: ZJ
authors.lastname: Dian
authors.affiliation: Department of Hematology, Yunnan Provincial First People's Hospital, Kunming, Yunnan 650032, China.
authors.forename: Yan
authors.identifier: 0000-0002-0801-9609
authors.initials: Y
authors.lastname: Wen
authors.affiliation: Institute of Clinical and Basic Medical Sciences, Yunnan Provincial First People's Hospital, Kunming, Yunnan 650032, China.
authors.forename: Fen
authors.identifier: 0000-0003-3836-5964
authors.initials: F
authors.lastname: Mei
authors.affiliation: Institute of Clinical and Basic Medical Sciences, Yunnan Provincial First People's Hospital, Kunming, Yunnan 650032, China.
authors.forename: Rui-Wei
authors.identifier: 0000-0002-9548-8824
authors.initials: RW
authors.lastname: Li
authors.affiliation: Institute of Clinical and Basic Medical Sciences, Yunnan Provincial First People's Hospital, Kunming, Yunnan 650032, China.
authors.forename: Ya-Lian
authors.identifier: 0000-0002-0942-6548
authors.initials: YL
authors.lastname: Sa
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D015703:Antigens, CD / Q000096:biosynthesis*; D001706:Biopsy; D001853:Bone Marrow / Q000378:metabolism / Q000473:pathology; D001854:Bone Marrow Cells / Q000378:metabolism / Q000473:pathology; D005260:Female; D015973:Gene Expression Regulation, Leukemic; D006801:Humans; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged; D009363:Neoplasm Proteins / Q000096:biosynthesis*
chemical_list: D015703:Antigens, CD; D009363:Neoplasm Proteins