title: RraAS1 inhibits the ribonucleolytic activity of RNase ES by interacting with its catalytic domain in Streptomyces coelicolor.
abstract: RraA is a protein inhibitor of RNase E, which degrades and processes numerous RNAs in Escherichia coli. Streptomyces coelicolor also contains homologs of RNase E and RraA, RNase ES and RraAS1/RraAS2, respectively. Here, we report that, unlike other RraA homologs, RraAS1 directly interacts with the catalytic domain of RNase ES to exert its inhibitory effect. We further show that rraAS1 gene deletion in S. coelicolor results in a higher growth rate and increased production of actinorhodin and undecylprodigiosin, compared with the wild-type strain, suggesting that RraAS1-mediated regulation of RNase ES activity contributes to modulating the cellular physiology of S. coelicolor.
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors.forename: Sojin
authors.initials: S
authors.lastname: Seo
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors.forename: Daeyoung
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors.forename: Wooseok
authors.initials: W
authors.lastname: Song
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors.forename: Jihune
authors.initials: J
authors.lastname: Heo
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors.forename: Minju
authors.initials: M
authors.lastname: Joo
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea.
authors.forename: Yeri
authors.initials: Y
authors.lastname: Lim
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea. jihyun82@cau.ac.kr.
authors.forename: Ji-Hyun
authors.initials: JH
authors.lastname: Yeom
authors.affiliation: Department of Life Science, Chung-Ang University, Seoul, 06974, Republic of Korea. kangseok@cau.ac.kr.
authors.forename: Kangseok
authors.initials: K
authors.lastname: Lee
mesh_terms: D000880:Anthraquinones / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism*; D020134:Catalytic Domain; D004722:Endoribonucleases / Q000037:antagonists & inhibitors* / Q000737:chemistry / Q000378:metabolism*; D017353:Gene Deletion; D015964:Gene Expression Regulation, Bacterial; D011353:Prodigiosin / Q000031:analogs & derivatives / Q000378:metabolism; D048372:Streptomyces coelicolor / Q000235:genetics* / Q000254:growth & development / Q000502:physiology*; D000095622:Benzoisochromanequinones
chemical_list: D000880:Anthraquinones; D001426:Bacterial Proteins; D004722:Endoribonucleases; D011353:Prodigiosin; C047347:undecylprodigiosin; C022537:ribonuclease E; C013390:actinorhodin; D000095622:Benzoisochromanequinones
keywords: RNase E; RNase ES; RraA; RraAS1; Streptomyces coelicolor