title: Characterization and phylogenetic analysis of Krüppel-like transcription factor (KLF) gene family in tree shrews (Tupaia belangeri chinensis).
abstract: Krüppel-like factors (KLFs) are a family of zinc finger transcription factors regulating embryonic development and diseases. The phylogenetics of KLFs has not been studied in tree shrews, an animal lineage with a closer relationship to primates than rodents. Here, we identified 17 KLFs from Chinese tree shrew (Tupaia belangeri chinensis). KLF proteins are highly conserved among humans, monkeys, rats, mice and tree shrews compared to zebrafish and chickens. The CtBP binding site, Sin3A binding site and nuclear localization signals are largely conserved between tree shrews and human beings. Tupaia belangeri (Tb) KLF5 contains several conserved post-transcriptional modification motifs. Moreover, the mRNA and protein expression patterns of multiple tbKLFs are tissue-specific . TbKLF5, like hKLF5, significantly promotes NIH3T3 cell proliferation in vitro. These results provide insight for future studies regarding the structure and function of the tbKLF gene family.
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.|Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.
authors.forename: Ming
authors.initials: M
authors.lastname: Shao
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.|Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, Yunnan, China.
authors.forename: Guang-Zhe
authors.initials: GZ
authors.lastname: Ge
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, Kunming, Yunnan, China.|Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Wen-Jing
authors.initials: WJ
authors.lastname: Liu
authors.affiliation: Medical Faculty, Kunming University of Science and Technology, Kunming, Yunnan, China.|Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Ji
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Hou-Jun
authors.initials: HJ
authors.lastname: Xia
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Zhao
authors.affiliation: Department of Laboratory Animal Science, Kunming Medical University, Kunming, Yunnan 650500, China.
authors.forename: Bao-Li
authors.initials: BL
authors.lastname: He
authors.affiliation: Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
authors.forename: Ceshi
authors.initials: C
authors.lastname: Chen
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001665:Binding Sites / Q000235:genetics; D015153:Blotting, Western; D002460:Cell Line; D049109:Cell Proliferation / Q000235:genetics; D020869:Gene Expression Profiling; D006801:Humans; D051741:Kruppel-Like Transcription Factors / Q000145:classification / Q000235:genetics* / Q000378:metabolism; D061986:MCF-7 Cells; D051379:Mice; D005810:Multigene Family; D041681:NIH 3T3 Cells; D010802:Phylogeny; D011499:Protein Processing, Post-Translational / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014418:Tupaiidae / Q000235:genetics*; D016335:Zinc Fingers / Q000235:genetics
chemical_list: D051741:Kruppel-Like Transcription Factors
keywords: KLF; KLF5; expression; motif; tree shrew