title: Alteration of Lysophosphatidylcholine-Related Metabolic Parameters in the Plasma of Mice with Experimental Sepsis.
abstract: Plasma concentration of lysophosphatidylcholine (LPC) was reported to decrease in patients with sepsis. However, the mechanisms of sepsis-induced decrease in plasma LPC levels are not currently well known. In mice subjected to cecal ligation and puncture (CLP), a model of polymicrobial peritoneal sepsis, we examined alterations in LPC-related metabolic parameters in plasma, i.e., the plasma concentration of LPC-related substances (i.e., phosphatidylcholine (PC) and lysophosphatidic acid (LPA)), and activities or levels in the plasma of some enzymes that can be involved in the regulation of plasma LPC concentration (i.e., secretory phospholipase A2 (sPLA2), lecithin:cholesterol acyltransferase (LCAT), acyl-CoA:lysophosphatidylcholine acyltransferase (LPCAT), and autotaxin (ATX)), as well as plasma albumin concentration. We found that levels of LPC and albumin and enzyme activities of LCAT, ATX, and sPLA2 were decreased, whereas levels of PC, LPA, and LPCAT1-3 were increased in the plasma of mice subjected to CLP. Bacterial peritonitis led to alterations in all the measured LPC-related metabolic parameters in the plasma, which could potentially contribute to sepsis-induced decrease in plasma LPC levels. These findings could lead to the novel biomarkers of sepsis.
authors.affiliation: Department of Pharmacology, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, South Korea.
authors.forename: Won-Gyun
authors.initials: WG
authors.lastname: Ahn
authors.affiliation: Department of Pharmacology, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, South Korea.|Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Jun-Sub
authors.initials: JS
authors.lastname: Jung
authors.affiliation: Department of Physiology, College of Medicine, Hallym University, Chuncheon, South Korea.
authors.forename: Hyeok Yil
authors.initials: HY
authors.lastname: Kwon
authors.affiliation: Department of Pharmacology, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do, 24252, South Korea. dksong@hallym.ac.kr.|Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon, South Korea. dksong@hallym.ac.kr.
authors.forename: Dong-Keun
authors.initials: DK
authors.lastname: Song
mesh_terms: D000217:Acyltransferases / Q000378:metabolism; D000818:Animals; D004195:Disease Models, Animal; D008244:Lysophosphatidylcholines / Q000097:blood / Q000378:metabolism*; D008246:Lysophospholipids / Q000378:metabolism; D008660:Metabolism; D051379:Mice; D010532:Peritoneal Diseases / Q000097:blood / Q000201:enzymology / Q000378:metabolism; D010740:Phospholipases / Q000378:metabolism; D018805:Sepsis / Q000097:blood* / Q000201:enzymology / Q000378:metabolism
chemical_list: D008244:Lysophosphatidylcholines; D008246:Lysophospholipids; D000217:Acyltransferases; D010740:Phospholipases
keywords: ATX; CLP; LPA; LPC; LPCAT; sepsis