title: Protocols for Studying Inhibition and Eradication of Bacterial Biofilms by Antimicrobial Peptides.
abstract: Many pathogenic microorganisms have the ability to form biofilms that are impervious to conventional antibiotics making these pathogens resistant to multiple antibiotics. This necessitates the development of novel antimicrobial compounds with less chance of resistance development and the ability to penetrate the extracellular polymer matrix of bacterial biofilms. In this report, simple assays to test the antibiofilm potential of antimicrobial peptides are described.
authors.affiliation: School of Chemical Sciences, The University of Auckland, 92019, Auckland, 1023, New Zealand. v.sarojini@auckland.ac.nz.
authors.forename: Vijayalekshmi
authors.initials: V
authors.lastname: Sarojini
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D001419:Bacteria / Q000187:drug effects* / Q000254:growth & development*; D018441:Biofilms / Q000187:drug effects*; D024881:Drug Resistance, Bacterial / Q000187:drug effects; D008826:Microbial Sensitivity Tests; D050296:Microbial Viability / Q000187:drug effects; D018613:Microscopy, Confocal
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides
keywords: Antibiotic resistance; Antimicrobial peptides; Bacterial biofilms; Confocal microscopy; Crystal violet stain; Live/Dead stain