title: The effect of tissue surface modification with collagenase and addition of TGF-β3 on the healing potential of meniscal tears repaired with tissue glues in vitro.
abstract: The aim of the current in vitro study was to investigate if tissue surface modification with collagenase and addition of the TGF-β3 can increase the number of cells present in meniscus tears repaired with the use of newly developed tissue adhesives based on isocyanate-terminated block copolymers. Cylindrical explants were harvested from the inner part of bovine menisci. To simulate a full-thickness tear, the central core of the explants was removed and glued back into the defect, with or without incubation in collagenase solution prior to gluing. The repair constructs were then cultured with or without addition of TGF-β3, and assessed for their histological appearance. The histological staining of the constructs confirmed that both developed adhesives were not cytotoxic. After 28 days, meniscus cells were present in direct contact with the glues. The addition of TGF-β3 to the culture medium resulted in the presence of cells that formed a sheath inside the simulated tear and in increased cell numbers at the edges of annulus of the explants. In the group in which the tissue was incubated in collagenase and cultured in medium containing TGF-β3, thicker layers of cells were observed. These results suggest that repairing the torn meniscus with tissue adhesives after pre-treatment of the tissue with collagenase and stimulation with TGF-β3 is a very promising treatment method, especially when treating the inner avascular part of the meniscus. Nevertheless, longer-term in vitro and in vivo studies are needed to confirm the beneficial effects of this combination therapy.
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedics, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands. agnieszka.bochynska@radboudumc.nl.|MIRA Institute for Biomedical Engineering and Technical Medicine, Department of Biomaterials Science and Technology, University of Twente, Enschede, The Netherlands. agnieszka.bochynska@radboudumc.nl.
authors.forename: Agnieszka Izabela
authors.initials: AI
authors.lastname: Bochyńska
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedics, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
authors.forename: Gerjon
authors.initials: G
authors.lastname: Hannink
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedics, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
authors.forename: Renate
authors.initials: R
authors.lastname: Verhoeven
authors.affiliation: MIRA Institute for Biomedical Engineering and Technical Medicine, Department of Biomaterials Science and Technology, University of Twente, Enschede, The Netherlands.|W.J. Kolff Institute, Department of Biomedical Engineering, University Medical Centre Groningen, University of Groningen, Groningen, The Netherlands.
authors.forename: Dirk W
authors.initials: DW
authors.lastname: Grijpma
authors.affiliation: Orthopaedic Research Laboratory, Department of Orthopaedics, Nijmegen Center for Molecular Life Sciences, Radboud University Medical Center, Nijmegen, The Netherlands.
authors.forename: Pieter
authors.initials: P
authors.lastname: Buma
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D002417:Cattle; D002465:Cell Movement; D017364:Collagenases / Q000737:chemistry*; D003470:Culture Media; D017953:Isocyanates / Q000737:chemistry; D008592:Menisci, Tibial / Q000166:cytology; D012421:Rupture / Q000473:pathology; D017550:Spectroscopy, Fourier Transform Infrared; D013499:Surface Properties; D000070600:Tibial Meniscus Injuries / Q000628:therapy*; D014014:Tissue Adhesives / Q000737:chemistry*; D023822:Tissue Engineering / Q000379:methods; D053782:Transforming Growth Factor beta3 / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D017953:Isocyanates; D014014:Tissue Adhesives; D053782:Transforming Growth Factor beta3; D017364:Collagenases