title: Use of Primary and Secondary Polyvinylamines for Efficient Gene Transfection.
abstract: Gene transfection with polymeric carrier remains a challenge; particularly, high transfection levels combined with low toxicity are hard to achieve. We herein revisit polyvinylamines, an old and neglected family of cationic polymers. They can be readily obtained by controlled hydrolysis of polyvinylamides prepared through (controlled) radical polymerization. A series of tailor-made and well-defined polyvinylamines bearing primary amino groups, and poly(N-methylvinylamine) bearing secondary amines, were evaluated for the transfection of cells with pDNA as a function of their molar mass, molar mass distribution, and degree of deacetylation. Unexpected high transfection levels, in combination with low cytotoxicity were recorded for both series. Surprisingly, a great impact of the molar mass was observed for the primary amine polyvinylamine series, whereas the results were mostly independent of molar mass or dispersity for the polymer bearing secondary amine. It was further established that a certain percentage of acetamide groups increased the transfection level, while maintaining low cytotoxicity. These results highlight for the first time the real potential of polyvinylamines as gene carriers, and make these polymers very attractive for further development in gene therapy.
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire, Equipe Chimie des Polymères, 4 Place Jussieu, F-75005 Paris, France.|Center for Education and Research on Macromolecules (CERM), Department of Chemistry, University of Liege (ULg) , Sart-Tilman, Allée de la Chimie 3, Bat. B6a, B-4000 Liège, Belgium.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Dréan
authors.affiliation: Center for Education and Research on Macromolecules (CERM), Department of Chemistry, University of Liege (ULg) , Sart-Tilman, Allée de la Chimie 3, Bat. B6a, B-4000 Liège, Belgium.
authors.forename: Antoine
authors.initials: A
authors.lastname: Debuigne
authors.affiliation: Centre de Biophysique Moléculaire, UPR4301 CNRS, Rue Charles Sadron, 45071 Orléans Cedex 2, France.
authors.forename: Cristine
authors.initials: C
authors.lastname: Goncalves
authors.affiliation: Center for Education and Research on Macromolecules (CERM), Department of Chemistry, University of Liege (ULg) , Sart-Tilman, Allée de la Chimie 3, Bat. B6a, B-4000 Liège, Belgium.
authors.forename: Christine
authors.initials: C
authors.lastname: Jérôme
authors.affiliation: Centre de Biophysique Moléculaire, UPR4301 CNRS, Rue Charles Sadron, 45071 Orléans Cedex 2, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Midoux
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire, Equipe Chimie des Polymères, 4 Place Jussieu, F-75005 Paris, France.
authors.forename: Jutta
authors.initials: J
authors.lastname: Rieger
authors.affiliation: Sorbonne Universités, UPMC Univ Paris 06, CNRS, Institut Parisien de Chimie Moléculaire, Equipe Chimie des Polymères, 4 Place Jussieu, F-75005 Paris, France.
authors.forename: Philippe
authors.identifier: 0000-0002-4919-0779
authors.initials: P
authors.lastname: Guégan
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D004337:Drug Carriers / Q000737:chemistry*; D015316:Genetic Therapy; D006367:HeLa Cells; D006801:Humans; D010957:Plasmids / Q000008:administration & dosage* / Q000235:genetics; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011145:Polyvinyls / Q000737:chemistry*
chemical_list: D004337:Drug Carriers; D011108:Polymers; D011145:Polyvinyls; C534904:polyvinylamine