title: Gene expression differences in Longissimus muscle of Nelore steers genetically divergent for residual feed intake.
abstract: Residual feed intake (RFI), a measure of feed efficiency (FE), is defined as the difference between the observed and the predictable feed intake considering size and growth of the animal. It is extremely important to beef production systems due to its impact on the allocation of land areas to alternative agricultural production, animal methane emissions, food demand and cost of production. Global differential gene expression analysis between high and low RFI groups (HRFI and LRFI: less and more efficient, respectively) revealed 73 differentially expressed (DE) annotated genes in Longissimus thoracis (LT) muscle of Nelore steers. These genes are involved in the overrepresented pathways Metabolism of Xenobiotics by Cytochrome P450 and Butanoate and Tryptophan Metabolism. Among the DE transcripts were several proteins related to mitochondrial function and the metabolism of lipids. Our findings indicate that observed gene expression differences are primarily related to metabolic processes underlying oxidative stress. Genes involved in the metabolism of xenobiotics and antioxidant mechanisms were primarily down-regulated, while genes responsible for lipid oxidation and ketogenesis were up-regulated in HRFI group. By using LT muscle, this study reinforces our previous findings using liver tissue and reveals new genes and likely tissue-specific regulators playing key-roles in these processes.
authors.affiliation: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil.|Division of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA.
authors.forename: Polyana C
authors.initials: PC
authors.lastname: Tizioto
authors.affiliation: Department of Animal Science, University of São Paulo/ESALQ, Piracicaba, São Paulo, Brazil.
authors.forename: Luiz L
authors.initials: LL
authors.lastname: Coutinho
authors.affiliation: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil.
authors.forename: Priscila S N
authors.initials: PS
authors.lastname: Oliveira
authors.affiliation: Department of Animal Science, University of São Paulo/ESALQ, Piracicaba, São Paulo, Brazil.
authors.forename: Aline S M
authors.initials: AS
authors.lastname: Cesar
authors.affiliation: Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, SP, Brazil.
authors.forename: Wellison J S
authors.initials: WJ
authors.lastname: Diniz
authors.affiliation: Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, SP, Brazil.
authors.forename: Andressa O
authors.initials: AO
authors.lastname: Lima
authors.affiliation: Department of Genetics and Evolution, Federal University of São Carlos, São Carlos, SP, Brazil.
authors.forename: Marina I
authors.initials: MI
authors.lastname: Rocha
authors.affiliation: Division of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA.|Informatics Institute, University of Missouri, Columbia, Missouri, 65211, USA.
authors.forename: Jared E
authors.initials: JE
authors.lastname: Decker
authors.affiliation: Division of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA.|Informatics Institute, University of Missouri, Columbia, Missouri, 65211, USA.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Schnabel
authors.affiliation: Department of Animal Science, University of São Paulo/ESALQ, Piracicaba, São Paulo, Brazil.
authors.forename: Gerson B
authors.initials: GB
authors.lastname: Mourão
authors.affiliation: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil.
authors.forename: Rymer R
authors.initials: RR
authors.lastname: Tullio
authors.affiliation: Embrapa Informática Agropecuária, Campinas, SP, Brazil.
authors.forename: Adhemar
authors.initials: A
authors.lastname: Zerlotini
authors.affiliation: Division of Animal Sciences, University of Missouri Columbia, Columbia, MO, USA.
authors.forename: Jeremy F
authors.initials: JF
authors.lastname: Taylor
authors.affiliation: Embrapa Pecuária Sudeste, São Carlos, SP, Brazil.
authors.forename: Luciana C A
authors.initials: LC
authors.lastname: Regitano
mesh_terms: D000821:Animal Feed; D000818:Animals; D002417:Cattle / Q000235:genetics*; D004063:Digestion; D051766:Early Growth Response Protein 1 / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D056726:Genetic Association Studies / Q000662:veterinary; D040961:Genetic Drift; D016678:Genome; D008055:Lipids / Q000737:chemistry; D008297:Male; D008697:Methane / Q000737:chemistry; D018482:Muscle, Skeletal / Q000378:metabolism*; D018384:Oxidative Stress; D010100:Oxygen / Q000737:chemistry; D010641:Phenotype; D017423:Sequence Analysis, RNA; D015262:Xenobiotics / Q000737:chemistry
chemical_list: D051766:Early Growth Response Protein 1; D008055:Lipids; D015262:Xenobiotics; D008697:Methane; D010100:Oxygen