title: Quantification of highly selective sigma-1 receptor antagonist CM304 using liquid chromatography tandem mass spectrometry and its application to a pre-clinical pharmacokinetic study.
abstract: An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for quantification of CM304, a novel and highly selective sigma-1 receptor antagonist that has recently entered into human clinical trials. A structural analogue of CM304, SN56, was used as the internal standard (IS). Chromatographic separation was achieved on an Acquity UPLC™ BEH C18 (1.7 μm, 2.1 mm × 50 mm) column using a mobile phase [water:methanol (0.1%v/v formic acid; 50:50, %v/v)] at a flow rate of 0.2 mL/min. Mass spectrometric detection was performed in the positive ionization mode with multiple reaction monitoring (MRM) using m/z transitions of 337 > 238 for CM304 and 319 > 220 for the IS. The method was found to be linear and reproducible with a regression coefficient consistently >0.99 for the calibration range of 3 to 3000 ng/mL. The extraction recovery ranged from 91.5 to 98.4% from spiked (7.5, 300 and 2526 ng/mL) plasma quality control samples. The precision (%RSD; 1.1 to 2.9%) and accuracy (%RE; -1.9 to 1.8%) were within acceptable limit. The validated method was successfully applied to a single dose oral and intravenous (I.V.) pharmacokinetic study of CM304 in rats. Following I.V. administration, the compound exhibited adequate exposure along with high extravascular distribution and insignificant amount of extra hepatic metabolism. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA.
authors.forename: Bonnie A
authors.identifier: http://orcid.org/0000-0002-0925-1338
authors.initials: BA
authors.lastname: Avery
authors.affiliation: Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA.
authors.forename: Pradeep K
authors.initials: PK
authors.lastname: Vuppala
authors.affiliation: Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA.
authors.forename: Seshulatha
authors.initials: S
authors.lastname: Jamalapuram
authors.affiliation: Department of Pharmaceutics and Drug Delivery, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA.
authors.forename: Abhisheak
authors.initials: A
authors.lastname: Sharma
authors.affiliation: Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA.
authors.forename: Christophe
authors.initials: C
authors.lastname: Mesangeau
authors.affiliation: Department of Radiology, Stanford University, Stanford, CA, 94305, USA.
authors.forename: Frederick T
authors.initials: FT
authors.lastname: Chin
authors.affiliation: Department of BioMolecular Sciences, School of Pharmacy, The University of Mississippi, University, MS, 38677, USA.
authors.forename: Christopher R
authors.initials: CR
authors.lastname: McCurdy
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000818:Animals; D001381:Azepines / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D052160:Benzothiazoles / Q000008:administration & dosage / Q000032:analysis / Q000097:blood*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004353:Drug Evaluation, Preclinical / Q000379:methods; D057230:Limit of Detection; D008297:Male; D051381:Rats; D017207:Rats, Sprague-Dawley; D017480:Receptors, sigma / Q000037:antagonists & inhibitors*; D053719:Tandem Mass Spectrometry / Q000379:methods*; D000097605:Sigma-1 Receptor
chemical_list: D001381:Azepines; D052160:Benzothiazoles; D017480:Receptors, sigma; D000097605:Sigma-1 Receptor; C000627013:CM304
keywords: CM304; UPLC-MS/MS; bioanalysis; cocaine addiction