title: The putative serine protease inhibitor (serpin) genes encoded on Echinococcus multilocularis genome and their expressions in metacestodal stage.
abstract: Two putative serpin genes were identified in Echinococcus multilocularis, in addition to the already reported serpinEmu, and were designated as serpin2Emu and serpin3Emu. Western blot analysis using polyclonal antibodies against serpinEmu, putative serpin2Emu protein, and putative serpin3Emu protein indicated that all three proteins were localized in both intracellular and excretory-secretory (ES) fractions of E. multilocularis metacestodes. In addition, immune staining of parasite tissue indicated that all three proteins were localized at the germinal layer.
authors.affiliation: Department of Parasitology, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan. Electronic address: mizuki_sasaki@asahikawa-med.ac.jp.
authors.forename: Mizuki
authors.initials: M
authors.lastname: Sasaki
authors.affiliation: Department of Parasitology, Asahikawa Medical University, 2-1-1-1, Midorigaoka-Higashi, Asahikawa, Hokkaido, 078-8510, Japan.
authors.forename: Yasuhito
authors.initials: Y
authors.lastname: Sako
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000378:metabolism; D015153:Blotting, Western; D048210:Echinococcus multilocularis / Q000235:genetics* / Q000378:metabolism; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D049751:Genome, Helminth / Q000235:genetics*; D015801:Helminth Proteins / Q000235:genetics; D008018:Life Cycle Stages; D015843:Serpins / Q000235:genetics* / Q000378:metabolism
chemical_list: D000909:Antibodies, Helminth; D015801:Helminth Proteins; D015843:Serpins
keywords: Alveolar echinococcosis; Echinococcus multilocularis; Metacestode; Serine protease inhibitor