title: [Comparative study of HIV-1/2 antibody confirmatory assay: Geenius™ versus INNO-LIA™].
abstract: OBJECTIVE
  The aim of the study is to compare two confirmatory tests for HIV-1/2 infection.
  
  
  METHODS
  A prospective study was carried out between 01/01/2015 and 12/31/2015. Serum samples with repeatedly positive results in the Antibody-Antigen-HIV-1/2 (Architect, Abbott) screening assay were included. The serum samples corresponding to new diagnosed cases were selected and were used to compare the two confirmatory assays: Geenius™ HIV-1/2 (Bio-Rad) and INNO-LIA™ HIV-1/2 score line-immunoassay (Innogene-tics®). The HIV-1 viral load (Cobas® AmpliPrepHIV, Ro-che) was performed in discordant or indeterminate cases.
  
  
  RESULTS
  Eight five samples were included. The results of both confirmatory assays were concordant in 80/85 samples: 53 HIV-1, 1 HIV-2, 25 negative and one indeterminate. Cohen's Kappa concordance coefficient between Geenius™ and INNO-LIA™ techniques was very high (0.878).
  
  
  CONCLUSIONS
  The concordance between the two assays is high. The procedure for Geenius™ is simple and fast. Geenius™ is a good alternative to include in the HIV-1/2 diagnostic algorithm.
authors.affiliation: Aitziber Aguinaga Pérez, Servicio de Microbiología Clínica. Complejo Hospitalario de Navarra, Calle de Irunlarrea 3, Pamplona, Navarra (CP: 31008). Spain. aitziber.aguinaga.perez@cfnavarra.es.
authors.forename: A
authors.initials: A
authors.lastname: Aguinaga
authors.forename: A
authors.initials: A
authors.lastname: Navascués
authors.forename: I
authors.initials: I
authors.lastname: Polo
authors.forename: C
authors.initials: C
authors.lastname: Ezpeleta
mesh_terms: D000465:Algorithms; D015483:HIV Antibodies / Q000032:analysis*; D015488:HIV Antigens / Q000032:analysis; D015658:HIV Infections / Q000175:diagnosis* / Q000276:immunology / Q000821:virology; D015497:HIV-1 / Q000276:immunology*; D015498:HIV-2 / Q000276:immunology*; D006801:Humans; D007118:Immunoassay; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D019562:Viral Load
chemical_list: D015483:HIV Antibodies; D015488:HIV Antigens