title: Characterization of glabridin/hydroxypropyl-β-cyclodextrin inclusion complex with robust solubility and enhanced bioactivity.
abstract: Various biological activities of glabridin have been reported in literature, however, the bioavailability and clinical application was limited by its low water solubility. In this study, we developed a novel inclusion complex of glabridin and hydroxypropyl-β-cyclodextrin (HP-β-CD) that features improved solubility and bioactivity. The formation of the inclusion complex was supported by data from FT-IR, PXRD, SEM and NMR. Specifically, the NMR results indicated that the aromatic ring of glabridin was merged into and located at the narrow side the cavity of HP-β-CD. The in vitro tests showed that the formation of the inclusion complex led to 9 times increase in the DPPH radical-scavenging capacity and 20 times increase in the tyrosinase inhibitory activity compared to the free glabridin at the same concentration. Therefore, the glabridin/HP-β-CD inclusion complex adds a promising strategy for the clinical application of glabridin in the future.
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China.
authors.forename: Yongqin
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Beijing Advanced Innovation Center for Soft Matter Science and Technology, Beijing University of Chemical Technology, Beijing, PR China.
authors.forename: Jin
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China.
authors.forename: Weiya
authors.initials: W
authors.lastname: Bei
authors.affiliation: College of Food Science and Nutritional Engineering, China Agricultural University, 100083, Beijing, PR China.
authors.forename: Yuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China.
authors.forename: Qipeng
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, PR China. Electronic address: starslh@163.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
mesh_terms: D000073738:2-Hydroxypropyl-beta-cyclodextrin / Q000737:chemistry*; D007529:Isoflavones / Q000737:chemistry*; D010636:Phenols / Q000737:chemistry*; D012995:Solubility; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D007529:Isoflavones; D010636:Phenols; D000073738:2-Hydroxypropyl-beta-cyclodextrin; C107601:glabridin
keywords: 2, 2-Diphenyl-1-picrylhydrazyl radical (PubChem CID: 74358); 2-Hydroxypropyl-β-cyclodextrin (PubChem CID: 14049689); 3, 4-Dihydroxy-l-phenylalanine (PubChem CID: 6047); Glabridin; Glabridin (PubChem CID: 124052); Hydroxypropyl-β-cyclodextrin; Inclusion complex