title: The exon junction complex: a lifelong guardian of mRNA fate.
abstract: During messenger RNA (mRNA) biogenesis and processing in the nucleus, many proteins are imprinted on mRNAs assembling them into messenger ribonucleoproteins (mRNPs). Some of these proteins remain stably bound within mRNPs and have a long-lasting impact on their fate. One of the best-studied examples is the exon junction complex (EJC), a multiprotein complex deposited primarily 24 nucleotides upstream of exon-exon junctions as a consequence of pre-mRNA splicing. The EJC maintains a stable, sequence-independent, hold on the mRNA until its removal during translation in the cytoplasm. Acting as a molecular shepherd, the EJC travels with mRNA across the cellular landscape coupling pre-mRNA splicing to downstream, posttranscriptional processes such as mRNA export, mRNA localization, translation, and nonsense-mediated mRNA decay (NMD). In this review, we discuss our current understanding of the EJC's functions during these processes, and expound its newly discovered functions (e.g., pre-mRNA splicing). Another focal point is the recently unveiled in vivo EJC interactome, which has shed new light on the EJC's location on the spliced RNAs and its intimate relationship with other mRNP components. We summarize new strides being made in connecting the EJC's molecular function with phenotypes, informed by studies of human disorders and model organisms. The progress toward understanding EJC functions has revealed, in its wake, even more questions, which are discussed throughout. WIREs RNA 2017, 8:e1411. doi: 10.1002/wrna.1411 For further resources related to this article, please visit the WIREs website.
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
authors.forename: Lauren A
authors.initials: LA
authors.lastname: Woodward
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
authors.forename: Justin W
authors.initials: JW
authors.lastname: Mabin
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
authors.forename: Pooja
authors.initials: P
authors.lastname: Gangras
authors.affiliation: Department of Molecular Genetics, The Ohio State University, Columbus, OH, USA.
authors.forename: Guramrit
authors.initials: G
authors.lastname: Singh
mesh_terms: D000818:Animals; D005091:Exons / Q000235:genetics*; D006801:Humans; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012261:Ribonucleoproteins / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D012261:Ribonucleoproteins; C026452:messenger ribonucleoprotein