title: A Trp-BODIPY cyclic peptide for fluorescence labelling of apoptotic bodies.
abstract: The rational design and synthesis of a Trp-BODIPY cyclic peptide for the fluorescent labelling of apoptotic bodies is described. Affinity assays, confocal microscopy and flow cytometry analysis confirmed the binding of the peptide to negatively-charged phospholipids associated with apoptosis, and its applicability for the detection and characterisation of subcellular structures released by apoptotic cells.
authors.affiliation: MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK. marc.vendrell@ed.ac.uk.
authors.forename: Ramon
authors.initials: R
authors.lastname: Subiros-Funosas
authors.affiliation: Department of Inorganic and Organic Chemistry, University of Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain.
authors.forename: Lorena
authors.initials: L
authors.lastname: Mendive-Tapia
authors.affiliation: Instituto Biofisika (CSIC, UPV/EHU) and Departamento de Bioquimica, Universidad del País Vasco, Campus de Leioa, Barrio de Sarriena s/n, 48940 Leioa, Bizkaia, Spain.
authors.forename: Jesus
authors.initials: J
authors.lastname: Sot
authors.affiliation: MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK. marc.vendrell@ed.ac.uk.
authors.forename: John D
authors.initials: JD
authors.lastname: Pound
authors.affiliation: MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK. marc.vendrell@ed.ac.uk.
authors.forename: Nicole
authors.initials: N
authors.lastname: Barth
authors.affiliation: Instituto Biofisika (CSIC, UPV/EHU) and Departamento de Bioquimica, Universidad del País Vasco, Campus de Leioa, Barrio de Sarriena s/n, 48940 Leioa, Bizkaia, Spain.
authors.forename: Yaiza
authors.initials: Y
authors.lastname: Varela
authors.affiliation: Instituto Biofisika (CSIC, UPV/EHU) and Departamento de Bioquimica, Universidad del País Vasco, Campus de Leioa, Barrio de Sarriena s/n, 48940 Leioa, Bizkaia, Spain.
authors.forename: Felix M
authors.initials: FM
authors.lastname: Goñi
authors.affiliation: MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK. marc.vendrell@ed.ac.uk.
authors.forename: Margaret
authors.initials: M
authors.lastname: Paterson
authors.affiliation: MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK. marc.vendrell@ed.ac.uk.
authors.forename: Christopher D
authors.initials: CD
authors.lastname: Gregory
authors.affiliation: Department of Inorganic and Organic Chemistry, University of Barcelona, Martí i Franquès 1-11, 08028 Barcelona, Spain and CIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Baldiri Reixac 10-12, 08028 Barcelona, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Albericio
authors.affiliation: MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK. marc.vendrell@ed.ac.uk.
authors.forename: Ian
authors.initials: I
authors.lastname: Dransfield
authors.affiliation: CIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Baldiri Reixac 10-12, 08028 Barcelona, Spain and Laboratory of Organic Chemistry, Faculty of Pharmacy, University of Barcelona, Baldiri Reixac 10-12, 08028 Barcelona, Spain. rlavilla@ub.edu.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Lavilla
authors.affiliation: MRC/UoE Centre for Inflammation Research, The University of Edinburgh, 47 Little France Crescent, EH16 4TJ Edinburgh, UK. marc.vendrell@ed.ac.uk.
authors.forename: Marc
authors.identifier: http://orcid.org/0000-0002-5392-9740
authors.initials: M
authors.lastname: Vendrell
mesh_terms: D001896:Boron Compounds / Q000737:chemistry*; D045744:Cell Line, Tumor; D000067128:Extracellular Vesicles / Q000737:chemistry*; D005434:Flow Cytometry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D010456:Peptides, Cyclic / Q000737:chemistry*; D013194:Staining and Labeling; D014364:Tryptophan / Q000737:chemistry*
chemical_list: C095489:4,4-difluoro-4-bora-3a,4a-diaza-s-indacene; D001896:Boron Compounds; D005456:Fluorescent Dyes; D010456:Peptides, Cyclic; D014364:Tryptophan