title: Mitochondrial Biomarkers Reflect Semen Quality: Results from the MARCHS Study in Chongqing, China.
abstract: Unexplained infertility requires that more sensitive and mechanism-based biomarkers should be developed and used independently of or in addition to conventional semen parameters for an infertility diagnosis. In the present study, semen samples were collected from young men participating in the Male Reproductive Health in Chongqing College students (MARCHS) cohort study in the follow-up stage in 2014. Conventional semen parameters were measured in all 656 participants, whereas sperm mitochondrial membrane potential (MMP), mitochondrial DNA copy number (mtDNAcn), mtDNA integrity and apoptotic parameters were measured among 627, 386, 362, and 628 participants, respectively. We found that sperm MMP was significantly positively correlated with all of conventional semen parameters including semen volume (r = 0.090, p = 0.025), sperm concentration (r = 0.301, p<0.01), total sperm count (r = 0.324, p<0.01), and progressive motility (r = 0.399, p<0.01); sperm MMP was also negatively correlated with Annexin V+ sperm (r = -0.553, p<0.01); mtDNAcn was significantly negatively correlated with sperm concentration (r = -0.214, p<0.01), total sperm count (r = -0.232, p<0.01), and progressive motility (r = -0.164, p = 0.01); mtDNA integrity was also significantly positively correlated with sperm concentration (r = 0.195, p<0.01), total sperm count (r = 0.185, p<0.01), and progressive motility (r = 0.106, p = 0.043). After adjusting for potential confounders, these relationships remained significant. Furthermore, we explored the potential effects of lifestyles on such mitochondrial biomarkers and found that the current drinkers displayed a higher level of sperm MMP; additionally, mt DNAcn was increased with age. The results indicated that certain mitochondrial biomarkers could serve as predictors of semen quality in a general population, and the study provides a baseline for the effects of population characteristics and lifestyles on such mitochondrial markers.
authors.affiliation: Department of Environmental Health, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Xi
authors.initials: X
authors.lastname: Ling
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zou
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Yang
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Niya
authors.initials: N
authors.lastname: Zhou
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Sun
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Jianfang
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Environmental Health, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Ziyuan
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cao
authors.affiliation: Institute of Toxicology, College of Preventive Medicine, Third Military Medical University, Chongqing, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ao
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism*; D002681:China; D004272:DNA, Mitochondrial / Q000235:genetics*; D006801:Humans; D008297:Male; D008928:Mitochondria / Q000378:metabolism*; D011446:Prospective Studies; D060728:Reproductive Health; D012661:Semen / Q000737:chemistry*; D055101:Semen Analysis / Q000379:methods*; D013081:Sperm Motility / Q000502:physiology*; D055815:Young Adult
chemical_list: D015415:Biomarkers; D004272:DNA, Mitochondrial