title: Purification and Characterization of Antioxidant Peptides of Pseudosciaena crocea Protein Hydrolysates.
abstract: Two peptides with antioxidant activity were isolated fromPseudosciaena croceaproteins.Pseudosciaena croceamuscle was hydrolyzed with neutral protease to obtainPseudosciaena croceaprotein hydrolysates (PCPH). After ultrafiltration through molecular weight cut-off membranes of 10, 5 and 3 kDa and assessment of free radical scavenging ability, the fraction (PCPH-IV) with the highest antioxidant activity was obtained. Several purification steps, i.e., ion exchange chromatography, gel filtration chromatography and reversed phase high performance liquid chromatography, were applied to further purify PCPH-IV. Two antioxidant peptides with the amino acid sequences Ser-Arg-Cys-His-Val and Pro-Glu-His-Trp were finally identified by LC-MS/MS.
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. znnfst@163.com.
authors.forename: Ningning
authors.initials: N
authors.lastname: Zhang
authors.affiliation: Fuzhou Municipal Finance Office, Fuzhou 350002, China. zhangchong2008cn@163.com.
authors.forename: Chong
authors.initials: C
authors.lastname: Zhang
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. cyyfst@163.com.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China. zbdfst@163.com.
authors.forename: Baodong
authors.initials: B
authors.lastname: Zheng
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D002374:Catalase / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D002845:Chromatography / Q000379:methods; D029941:Fish Proteins / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D005979:Glutathione Peroxidase / Q000378:metabolism; D056945:Hep G2 Cells; D006801:Humans; D006861:Hydrogen Peroxide / Q000037:antagonists & inhibitors / Q000494:pharmacology; D009132:Muscles / Q000737:chemistry; D010455:Peptides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D010473:Perciformes / Q000378:metabolism*; D010851:Picrates / Q000037:antagonists & inhibitors; D011492:Protein Hydrolysates / Q000737:chemistry* / Q000494:pharmacology; D013482:Superoxide Dismutase / Q000378:metabolism; D013481:Superoxides / Q000037:antagonists & inhibitors; D053719:Tandem Mass Spectrometry; D014462:Ultrafiltration
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D029941:Fish Proteins; D010455:Peptides; D010851:Picrates; D011492:Protein Hydrolysates; D013481:Superoxides; D006861:Hydrogen Peroxide; C004931:1,1-diphenyl-2-picrylhydrazyl; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Pseudosciaena crocea; amino acid sequence; antioxidant; chromatographic separation; peptide purification