title: Effect of dexmedetomidine on cerebral ischemia-reperfusion rats by activating mitochondrial ATP-sensitive potassium channel.
abstract: The aim of the study reported here was to evaluate whether the mitochondrial ATP-sensitive potassium (mitoKATP) channel could participate in the effect of dexmedetomidine on cerebral ischemia-reperfusion (I/R) rats. Forty rats were randomly assigned into 5 groups: sham operation (S) group; cerebral I/R group; dexmedetomidine (D) group; 5-hydroxydecanoate (5-HD) group; 5-HD + D group. The cerebral I/R were produced by 2 h right middle cerebral artery occlusion followed by 24 h reperfusion. Dexmedetomidine (50μg/kg) was injected intraperitoneally before ischemia and after the onset of reperfusion. 5-HD (30 mg/kg) was injected intraperitoneally at 1 h before ischemia. The neurological deficit score (NDS) and the levels of super oxide dismutase (SOD), malondialdehyde (MDA), myeloperoxidase (MPO), Interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α) were evaluated. Compared to group S, NDS and the levels of MDA, MPO, IL-6 and TNF-α were significantly higher, and SOD levels were significantly lower in the other groups (P < 0.05). Compared to group I/R,NDS and the levels of MDA, MPO, IL-6 and TNF-α were significantly lower, and SOD level was significantly higher in group D (P < 0.05). Compared to group D, NDS and the levels of MDA, MPO, IL-6 and TNF-α were significantly higher, and SOD level was significantly lower in group5-HD + D (P < 0.05). The activation of the mitoKATPchannel could contribute to the protective effect of dexmedetomidine on rats induced by focal cerebral ischemia-reperfusion injury.
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, No. 2 of Jingba road of Jinshui District, Zhengzhou, 450014, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yuan
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, No. 2 of Jingba road of Jinshui District, Zhengzhou, 450014, China.
authors.forename: Hongguang
authors.initials: H
authors.lastname: Fu
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, No. 2 of Jingba road of Jinshui District, Zhengzhou, 450014, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Sun
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, No. 2 of Jingba road of Jinshui District, Zhengzhou, 450014, China.
authors.forename: Shubiao
authors.initials: S
authors.lastname: Wu
authors.affiliation: Department of Anesthesiology, The Second Affiliated Hospital of Zhengzhou University, No. 2 of Jingba road of Jinshui District, Zhengzhou, 450014, China. 455275093@qq.com.
authors.forename: Tieli
authors.identifier: 0000-0002-2494-1302
authors.initials: T
authors.lastname: Dong
mesh_terms: D000316:Adrenergic alpha-Agonists / Q000494:pharmacology*; D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D001923:Brain Chemistry / Q000187:drug effects; D016207:Cytokines / Q000378:metabolism; D003652:Decanoic Acids / Q000494:pharmacology; D020927:Dexmedetomidine / Q000494:pharmacology*; D006880:Hydroxy Acids / Q000494:pharmacology; D054086:KATP Channels / Q000819:agonists*; D008297:Male; D020768:Middle Cerebral Artery; D008928:Mitochondria / Q000187:drug effects / Q000378:metabolism*; D009422:Nervous System Diseases / Q000209:etiology / Q000523:psychology; D018384:Oxidative Stress / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000188:drug therapy* / Q000378:metabolism
chemical_list: D000316:Adrenergic alpha-Agonists; D000975:Antioxidants; D016207:Cytokines; D003652:Decanoic Acids; D006880:Hydroxy Acids; D054086:KATP Channels; C052853:5-hydroxydecanoic acid; D020927:Dexmedetomidine
keywords: Anti-inflammatory reaction; Anti-oxidative stress reaction; Cerebral ischemia-reperfusion; Dexmedetomidine; Mitochondrial ATP-sensitive potassium channel