title: Molecular and functional characterization of pigeon (Columba livia) tumor necrosis factor receptor-associated factor 3.
abstract: Tumor necrosis factor receptor-associated factor 3 (TRAF3) plays a key antiviral role by promoting type I interferon production. We cloned the pigeon TRAF3 gene (PiTRAF3) according to its predicted mRNA sequence to investigate its function. The 1704-bp full-length open reading frame encodes a 567-amino acid protein. One Ring finger, two TRAF-type Zinc fingers, one Coiled coil, and one MATH domain were inferred. RT-PCR showed that PiTRAF3 was expressed in all tissues, with relatively weak expression in the heart and liver. In HEK293T cells, over-expression of wild-type, △Ring, △Zinc finger, and △Coiled coil PiTRAF3, but not a △MATH form, significantly increased IFN-β promoter activity. Zinc finger and Coiled coil domains were essential for NF-κB activation. In chicken HD11 cells, PiTRAF3 increased IFN-β promoter activity and four domains were all contributing. R848 stimulation of pigeon peripheral blood mononuclear cells and splenocytes significantly increased expression of PiTRAF3 and the inflammatory cytokine genes CCL5, IL-8, and IL-10. These data demonstrate TRAF3's innate immune function and improve understanding of its involvement in poultry antiviral defense.
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Xilong
authors.initials: X
authors.lastname: Kang
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Dan
authors.initials: D
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Huijuan
authors.initials: H
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China.
authors.forename: Yaxin
authors.initials: Y
authors.lastname: Guo
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China. Electronic address: zmpan@yzu.edu.cn.
authors.forename: Zhiming
authors.initials: Z
authors.lastname: Pan
authors.affiliation: Key Laboratory of Zoonoses in Jiangsu Province, Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou 225009, PR China. Electronic address: xajiao@yzu.edu.cn.
authors.forename: Xinan
authors.initials: X
authors.lastname: Jiao
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000235:genetics / Q000378:metabolism*; D002645:Chickens; D003001:Cloning, Molecular; D010856:Columbidae / Q000276:immunology*; D057809:HEK293 Cells; D006801:Humans; D007113:Immunity, Innate; D007249:Inflammation / Q000276:immunology*; D016899:Interferon-beta / Q000235:genetics; D016328:NF-kappa B / Q000378:metabolism; D011401:Promoter Regions, Genetic / Q000235:genetics; D011487:Protein Conformation; D000072417:Protein Domains / Q000235:genetics; D015202:Protein Engineering; D048008:TNF Receptor-Associated Factor 3 / Q000235:genetics / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D030161:Avian Proteins; D016328:NF-kappa B; D048008:TNF Receptor-Associated Factor 3; D016899:Interferon-beta
keywords: Cloning; Function; Identification; Pigeon; Tumor necrosis factor receptor-associated factor 3