title: [Effect of fluorochloridone on oxidative stress in primary co-cultured sertoli-germ cell of rat].
abstract: Objective:To explore effect of fluorochloridone on primary co-cultured sertoli-germ cell of rat and its possible mechanism.Methods:primary co-cultured sertoli-germ cell was made by two steps of en-zyme digestion with SD rat testes, after 24h of Sertoli-germ cell isolation, A 0.1% DMSO solvent control group and three FLC exposure groups (10-6、10-7、10-8mol/L) were selected, cultured cell for 24h, then MTT assay and index detection of oxidative stress were performed.Results:The mortality of primary co-cultured sertoli-germ cell exposed to 10-6mol/L FLC was significantly higher than control group and FLC exposure groups (10-7、10-8mol/L) (P<0.05) . 10-6mol/L FLC reduced enzyme activity of CAT, SOD and GSH. Px, depressed GSH level, el-evated MDA level, and had significant difference than control group and FLC exposure groups(10-7、10-8mol/L) (P<0.05) . 10-7mol/L FLC decreased enzyme activity of CAT than control group, depressed enzyme activity of SOD than 10-8mol/L FLC, lowered GSH level than control group and FLC exposure groups (10-8mol/L) , and had statistical difference (P<0.05) .Conclusion:FLC can damage primary co-cultured sertoli-germ cell of rat, its possible mechanism is relevant to oxidative stress.
authors.affiliation: Department of Medical Technology, Xiamen Medical University, Xiamen 361008, China.
authors.forename: S L
authors.initials: SL
authors.lastname: Wu
authors.forename: L R
authors.initials: LR
authors.lastname: Xu
authors.forename: Z J
authors.initials: ZJ
authors.lastname: Zhou
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D018920:Coculture Techniques; D005854:Germ Cells; D008297:Male; D018384:Oxidative Stress; D011760:Pyrrolidinones / Q000633:toxicity*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012708:Sertoli Cells
chemical_list: D011760:Pyrrolidinones; C077976:raiser