title: Selective in vitro photothermal nano-therapy of MRSA infections mediated by IgG conjugated gold nanoparticles.
abstract: There are serious systemic infections associated with methicillin-resistant Staphylococcus aureus (MRSA) and several other types of bacteria leading to the deaths of millions of people globally. This type of mortality is generally caused by the increasing number of antibiotic-resistant organisms, a consequence of evolution via natural selection. After the synthesis of gold nanoparticles (GNPs) by wet chemistry, bio-functionalization with IgG molecules was performed. Following administration of IgG-GNPs to MRSA cultures at various concentrations and various incubation time laser irradiation was performed. To assess the selectivity and specificity of the proposed treatment the following methods were used: flow cytometry, contrast phase microscopy, and by fluorescence microscopy. The results in our study indicate that following administration of IgG-GNPs biomolecule an extended and selective bacterial death occurs following laser irradiation in a dose dependent manner. Therefore, the new findings might impel studies on these antibacterial nanomaterials and their biological and medical applications.
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.|Department of Surgery, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Lucian
authors.initials: L
authors.lastname: Mocan
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.
authors.forename: Cristian
authors.initials: C
authors.lastname: Matea
authors.affiliation: Department of Pathology, University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine 3-5 Manastur Street, 400372 Cluj-Napoca, Romania.
authors.forename: Flaviu A
authors.initials: FA
authors.lastname: Tabaran
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.|Department of Gastroenterology, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Ofelia
authors.initials: O
authors.lastname: Mosteanu
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.|Department of Gastroenterology, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Teodora
authors.initials: T
authors.lastname: Pop
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.|Department of Surgery, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Cosmin
authors.initials: C
authors.lastname: Puia
authors.affiliation: Department of Internal Medicine, University of Medicine and Pharmacy, "Iuliu Hatieganu", Clinicilor 3-5, Cluj-Napoca, Romania.
authors.forename: Lucia
authors.initials: L
authors.lastname: Agoston-Coldea
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.|Department of Surgery, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Diana
authors.initials: D
authors.lastname: Gonciar
authors.affiliation: Department of Epidemiology, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.
authors.forename: Erszebet
authors.initials: E
authors.lastname: Kalman
authors.affiliation: Department of Neonatology, "Iuliu Hatieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Zaharie
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.|Department of Surgery, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Cornel
authors.initials: C
authors.lastname: Iancu
authors.affiliation: Nanomedicine Department, Regional Institute of Gastroenterology and Hepatology Octavian Fodor Cluj, Romania.|Department of Physiology, University of Medicine and Pharmacy, "Iuliu Hatieganu", Croitorilor 19-21, Cluj-Napoca, Romania.
authors.forename: Teodora
authors.initials: T
authors.lastname: Mocan
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D002469:Cell Separation; D002470:Cell Survival; D016503:Drug Delivery Systems; D005434:Flow Cytometry; D006046:Gold / Q000737:chemistry*; D007074:Immunoglobulin G / Q000737:chemistry*; D008027:Light; D053768:Metal Nanoparticles / Q000737:chemistry*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects*; D008826:Microbial Sensitivity Tests; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008856:Microscopy, Fluorescence; D008858:Microscopy, Phase-Contrast; D053761:Nanocomposites / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013203:Staphylococcal Infections / Q000188:drug therapy*
chemical_list: D000900:Anti-Bacterial Agents; D007074:Immunoglobulin G; D006046:Gold