title: CA3 hippocampal field: Cellular changes and its relation with blood nitro-oxidative stress reveal a balancing function of CA3 area in rats exposed to repetead restraint stress.
abstract: Rats exposed to repeated restraint stress exhibit structural and functional deficits in hippocampus that are similar to those observed in patients with depressive illnesses. Blood corticosterone concentrations are proportionally increased with catalase and glutathione-peroxidase activity and are inversely proportional with 3-nitrotyrosine concentrations.Cytochrome c oxidase, adenosin tryphosphatase and monoamine oxidase activities of CA3 hippocampal field mark a stress-time dependent decrease. Acridine-orange labeling of the CA3 field reveals an enhancing green fluorescence of glyocites in stress conditions. After three days of restraint stress, the secretory activity of CA3 neurons did not show significant decrease, and neurons appeared with normal shapes and distribution. CA3 neurons after seven days of restraint stress have marked a slight decrease of secretory activity. In contrast to a well-preserved histological appearance of the CA3 neurons, local and blood stress-related reactions are observed. CA3-glial activation and disturbance of blood oxidative homeostasis are tandem processes during three and seven days of RS. This study depicts the balancing role of CA3 area in time-varying stress conditions.
authors.affiliation: Babes-Bolyai University, Cluj-Napoca, Romania; Institute of Biological Research, Cluj-Napoca, Romania; NIRD of Isotopic and Molecular Technologies, Cluj-Napoca, Romania. Electronic address: vlad.al.toma@gmail.coma.
authors.forename: Vlad Al
authors.initials: VA
authors.lastname: Toma
authors.affiliation: Babes-Bolyai University, Cluj-Napoca, Romania; Institute of Biological Research, Cluj-Napoca, Romania; NIRD of Isotopic and Molecular Technologies, Cluj-Napoca, Romania.
authors.forename: Anca D
authors.initials: AD
authors.lastname: Farcas
authors.affiliation: Babes-Bolyai University, Cluj-Napoca, Romania.
authors.forename: Marcel
authors.initials: M
authors.lastname: Parvu
authors.affiliation: Babes-Bolyai University, Cluj-Napoca, Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Silaghi-Dumitrescu
authors.affiliation: Institute of Biological Research, Cluj-Napoca, Romania.
authors.forename: Ioana
authors.initials: I
authors.lastname: Roman
mesh_terms: D000818:Animals; D056654:CA3 Region, Hippocampal / Q000201:enzymology / Q000378:metabolism*; D005260:Female; D009457:Neuroglia / Q000378:metabolism; D009569:Nitric Oxide / Q000097:blood*; D018384:Oxidative Stress; D017208:Rats, Wistar; D012149:Restraint, Physical; D013315:Stress, Psychological / Q000378:metabolism*
chemical_list: D009569:Nitric Oxide
keywords: Blood; CA3 field; Gliocytes; Stress