title: A Study on the Anticarcinogenic Effects of Calcium Fructoborate.
abstract: Evidences about the preventive and therapeutic effects of boron compounds on cancer have been increasing in the last years. Although calcium fructoborate (CaFB) is used as a nutritional supplement, data about its preventive and therapeutic effects on neoplastic transformations are limited. In the present study, the various concentrations of CaFB were applied to the MDA-MB-231 metastatic breast cancer cell line. First, we examined the cytotoxic effect and IC50value of CaFB by MTT assay. For the evaluation of the DNA damage, apoptosis and metastatic potential, expression levels of ATM, pATM, PARP, p53, p-p53, caspase-3, caspase-9, and VEGF were investigated by using immunoblotting and immunohistochemical methods. Cell viability was significantly reduced at 50 μM CaFB treatment. pATM, p-p53, and caspase-9 levels increased significantly in all groups; furthermore, there was approximately 12.5-, 2.4-, and 10.7-fold increase, respectively, for 100 μM CaFB treatment. ATM and p53 levels did not change with CaFB treatment, but PARP levels significantly 2.5-fold decreased. While VEGF immunoreactivity decreased in all groups, significant increase in caspase-3 immunoreactivity was observed only in the group treated with 50 μM CaFB (p < 0,001). Our results imply that CaFB may have therapeutic potential as well as preventive benefits in cancer.
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
authors.forename: Burcu Erbaykent
authors.initials: BE
authors.lastname: Tepedelen
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey. mehmet.korkmaz@cbu.edu.tr.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Korkmaz
authors.affiliation: Department of Anatomy, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
authors.forename: Ertugrul
authors.initials: E
authors.lastname: Tatlisumak
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
authors.forename: Elgin Türköz
authors.initials: ET
authors.lastname: Uluer
authors.affiliation: Department of Medical Pharmacology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
authors.forename: Ercüment
authors.initials: E
authors.lastname: Ölmez
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Değerli
authors.affiliation: Department of Medical Biology, Faculty of Medicine, Celal Bayar University, 45030, Manisa, Turkey.
authors.forename: Elif
authors.initials: E
authors.lastname: Soya
authors.affiliation: Department of Histology and Embryology, Faculty of Medicine, İzmir University of Economics, 35330, İzmir, Turkey.
authors.forename: Sevinç
authors.initials: S
authors.lastname: İnan
mesh_terms: D016588:Anticarcinogenic Agents / Q000494:pharmacology*; D001881:Borates / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D004354:Drug Screening Assays, Antitumor; D005260:Female; D005632:Fructose / Q000031:analogs & derivatives* / Q000494:pharmacology; D006801:Humans; D009363:Neoplasm Proteins / Q000378:metabolism
chemical_list: D016588:Anticarcinogenic Agents; D001881:Borates; D009363:Neoplasm Proteins; C507177:calcium fructoborate; D005632:Fructose
keywords: Apoptosis; Boron; Calcium fructoborate; DNA damage; MDA-MB - 231; VEGF