title: Establishment and characterization of Caspian horse fibroblast cell bank in Iran.
abstract: Caspian horse, a rare horse breed found in 1965 by Louise Firouz in northern Iran, is a small horse which is reported to be in danger of extinction in its original homeland. There seems to be a great need to prevent extinction of this valuable horse. In this study, 51 fibroblast cell lines from Caspian horse ear marginal tissue were successfully established by sampling 60 horses using primary explant technique. Cells were authenticated and growth curve was plotted. According to results obtained, population doubling time (PDT) was calculated 23 ± 0.5 h for all cell lines. Multiplex polymerase chain reaction (multiplex PCR) revealed that cell lines had no cross-contamination with other species. Bacteria, fungi, and mycoplasma contamination were checked using standard methods such as PCR, direct culture, and Hoechst staining. In addition to providing a valuable source for genomic, postgenomic, and somatic cloning researches, the established cell lines would preserve Caspian horse genetic resources. It will also create an accessible database for researchers.
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Daneshvar Amoli
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Nazanin
authors.initials: N
authors.lastname: Mohebali
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Parvaneh
authors.initials: P
authors.lastname: Farzaneh
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.|Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in Biology, University of Science and Culture, Tehran, Iran.
authors.forename: Seyed Abolhassan
authors.initials: SA
authors.lastname: Shahzadeh Fazeli
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Laleh
authors.initials: L
authors.lastname: Nikfarjam
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Sepideh
authors.initials: S
authors.lastname: Ashouri Movasagh
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Moradmand
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Meysam
authors.initials: M
authors.lastname: Ganjibakhsh
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Nasimian
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Mehrnaz
authors.initials: M
authors.lastname: Izadpanah
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Faezeh
authors.initials: F
authors.lastname: Vakhshiteh
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Neda Sadat
authors.initials: NS
authors.lastname: Gohari
authors.affiliation: Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
authors.forename: Najmeh Sadat
authors.initials: NS
authors.lastname: Masoudi
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Farghadan
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran.
authors.forename: Shiva
authors.initials: S
authors.lastname: Mohamadi Moghanjoghi
authors.affiliation: Iran Equestrian Federation, University of Applied Science and Technology, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Khalili
authors.affiliation: Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, P. O. Box: 1551916111, Tehran, Iran. Khaledi@ibrc.ir.|Department of Agriculture, Yadegar-e-Imam Khomeini (rah), Shahr-e- rey Branch, Islamic Azad university, Tehran, Iran. Khaledi@ibrc.ir.
authors.forename: Kourosh J
authors.initials: KJ
authors.lastname: Khaledi
mesh_terms: D000818:Animals; D002460:Cell Line; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D033481:Chromosomes, Mammalian / Q000235:genetics; D005260:Female; D005347:Fibroblasts / Q000166:cytology*; D006736:Horses; D007150:Immunohistochemistry; D007492:Iran; D008297:Male; D016133:Polymerase Chain Reaction; D014015:Tissue Banks; D014162:Transfection
keywords: Caspian horse; Cell banking; Conservation; Fibroblast cell line