title: Plasma concentrations of CCL3 and CCL4 in the cardiac and digestive clinical forms of chronic Chagas disease.
abstract: The aim of this study was to investigate the plasma levels of the CCL3 and CCL4 chemokines in patients with the cardiac and digestive clinical forms of chronic Chagas disease and in cardiac patients with and without left ventricular systolic dysfunction (LVSD). Plasma samples from 75 patients were evaluated by enzyme-linked immunosorbent assay (ELISA) to confirm infection by T. cruzi. Plasma levels of the CCL3 and CCL4 chemokines were measured using Milliplex® MAP assay (Millipore). There were no significant differences in the levels of CCL3 and CCL4 between patients with the digestive and cardiac clinical forms of Chagas disease. Moreover, no significant differences were found between patients without LVSD and those with LVSD. Higher CCL3 and CCL4 plasma levels were found in patients with LVSD compared to those with the digestive form of the disease. The CCL3 and CCL4 chemokines might not be involved in differential susceptibility to the digestive and cardiac clinical forms of chronic Chagas disease, and it seems they do not influence the development of LVSD.
authors.affiliation: Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Amanda Priscila
authors.initials: AP
authors.lastname: de Oliveira
authors.affiliation: Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Christiane Maria
authors.initials: CM
authors.lastname: Ayo
authors.affiliation: Immunomorphology Laboratory, Biology Department, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Kallyne Kioko Oliveira
authors.initials: KK
authors.lastname: Mimura
authors.affiliation: Immunomorphology Laboratory, Biology Department, Universidade Estadual Paulista Júlio de Mesquita Filho, Rua Cristóvão Colombo, 2265, 15054-000 São José do Rio Preto, SP, Brazil.
authors.forename: Sonia Maria
authors.initials: SM
authors.lastname: Oliani
authors.affiliation: Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Cássia Rubia
authors.initials: CR
authors.lastname: Bernardo
authors.affiliation: Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Ana Vitória Silveira
authors.initials: AV
authors.lastname: Camargo
authors.affiliation: Surgery Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil; Hospital de Base, Fundação Faculdade Regional de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Luís Sérgio
authors.initials: LS
authors.lastname: Ronchi
authors.affiliation: Surgery Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil; Hospital de Base, Fundação Faculdade Regional de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Aldenis Albaneze
authors.initials: AA
authors.lastname: Borim
authors.affiliation: Surgery Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil; Hospital de Base, Fundação Faculdade Regional de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Eumildo
authors.initials: E
authors.lastname: de Campos Júnior
authors.affiliation: Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Cinara Cássia
authors.initials: CC
authors.lastname: Brandão de Mattos
authors.affiliation: Department of Epidemiology and Public Health, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Lilian
authors.initials: L
authors.lastname: Castiglioni
authors.affiliation: Department of Cardiology and Cardiovascular Surgery, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil; Hospital de Base, Fundação Faculdade Regional de Medicina de São José do Rio Preto, São José do Rio Preto, São Paulo, Brazil.
authors.forename: Reinaldo Bulgarelli
authors.initials: RB
authors.lastname: Bestetti
authors.affiliation: Center for the Investigation of Microorganisms, Department of Dermatological, Infectious and Parasitic Diseases, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil.
authors.forename: Carlos Eugênio
authors.initials: CE
authors.lastname: Cavasini
authors.affiliation: Immunogenetics Laboratory, Molecular Biology Department, Faculdade de Medicina de São José do Rio Preto, Avenida Brigadeiro Faria Lima, 5416, 15090-000 São José do Rio Preto, SP, Brazil. Electronic address: luiz.demattos@famerp.br.
authors.forename: Luiz Carlos
authors.initials: LC
authors.lastname: de Mattos
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014355:Chagas Disease / Q000097:blood*; D054405:Chemokine CCL3 / Q000097:blood*; D054407:Chemokine CCL4 / Q000097:blood*; D002908:Chronic Disease; D005260:Female; D005767:Gastrointestinal Diseases / Q000097:blood*; D006801:Humans; D008297:Male; D008875:Middle Aged; D014349:Trypanosoma cruzi; D018487:Ventricular Dysfunction, Left / Q000097:blood*
chemical_list: C515429:CCL3 protein, human; C066507:CCL4 protein, human; D054405:Chemokine CCL3; D054407:Chemokine CCL4
keywords: CCL3 chemokine; CCL4 chemokine; Chagas disease; Chronic Chagas heart disease