title: Differentiation of frogs from two populations belonging to the Pelophylax esculentus complex by LC-MS/MS comparison of their skin peptidomes.
abstract: LC-MS/MS was applied to establish the composition of the skin peptidome of a Slovenian green frog belonging to the Pelophylax esculentus complex. As this was similar to the peptidome of the Moscow population of Pelophylax ridibundus, it allowed us to identify the Slovenian frog from the Pelophylax esculentus complex as Pelophylax ridibundus. The sequences of six new peptides from the brevinin 2 family are reported for the first time on the basis of manual interpretation of their tandem mass spectra. The structural similarity of the brevinin 2 peptides from the Moscow and Slovenian populations of Pelophylax ridibundus enables peptides from this family to be utilized as biomarkers for Pelophylax ridibundus inter- and intraspecies differentiation, and the proposed approach can be used as an analytical tool for differentiating the corresponding species and populations. The potential biological activities of the novel peptides were estimated by 2D mass mapping. The results allowed us to classify all of the available peptides belonging to the brevinin 2 family. Graphical Abstract Intraspecies identification within the green frog complex.
authors.affiliation: Department of Chemistry, Moscow State University, Leninskie Gori 1/3, 119991, Moscow, Russian Federation.
authors.forename: Tatiana Yu
authors.initials: TY
authors.lastname: Samgina
authors.affiliation: Department of Chemistry-Biomedical Center, Analytical Chemistry, Uppsala University, Uppsala, 75124, Sweden.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Artemenko
authors.affiliation: Department of Chemistry-Biomedical Center, Analytical Chemistry, Uppsala University, Uppsala, 75124, Sweden.
authors.forename: Jonas
authors.initials: J
authors.lastname: Bergquist
authors.affiliation: Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, 1000, Ljubljana, Slovenia.
authors.forename: Polonca
authors.initials: P
authors.lastname: Trebse
authors.affiliation: Faculty of Education, University of Ljubljana, Kardeljeva ploščad 16, 1000, Ljubljana, Slovenia.
authors.forename: Gregor
authors.initials: G
authors.lastname: Torkar
authors.affiliation: Department of Chemistry, Moscow State University, Leninskie Gori 1/3, 119991, Moscow, Russian Federation.
authors.forename: Miriam D
authors.initials: MD
authors.lastname: Tolpina
authors.affiliation: Department of Chemistry, Moscow State University, Leninskie Gori 1/3, 119991, Moscow, Russian Federation. a.lebedev@org.chem.msu.ru.
authors.forename: Albert T
authors.initials: AT
authors.lastname: Lebedev
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002853:Chromatography, Liquid / Q000379:methods*; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D011898:Ranidae / Q000378:metabolism*; D012867:Skin / Q000378:metabolism*; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D010455:Peptides
keywords: Brevinins; Frog peptides; Green frogs; Natural products; Peptide sequencing; Tandem mass spectrometry