title: Microwave-assisted extraction versus Soxhlet extraction to determine triterpene acids in olive skins.
abstract: Microwave-assisted extraction is compared with a more classical technique, Soxhlet extraction, to determine the content of triterpene acids in olive skins. The samples used in their original unmilled state and milled were extracted with ethyl acetate or methanol as solvents. The optimized operating conditions (e.g., amount and type of solvent, and time and temperature of extractions) to attain the better extraction yields have been established. For the identification and quantitation of the target compounds, an ultra high performance liquid chromatography with tandem mass spectrometry method was employed. The best results were achieved using the microwave-assisted extraction technique, which was much faster than the Soxhlet extraction method, and showed higher efficiency in the extraction of the triterpenic acids (oleanolic and maslinic).
authors.affiliation: Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Fernandez-Pastor
authors.affiliation: Centro "Venta del Llano" del Instituto Andaluz de Investigación y Formación Agraria, Pesquera, Agroalimentaria y de la Producción Ecológica (IFAPA), Mengíbar, Jaén, Spain.
authors.forename: Antonia
authors.initials: A
authors.lastname: Fernandez-Hernandez
authors.affiliation: Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Perez-Criado
authors.affiliation: Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Rivas
authors.affiliation: Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Martinez
authors.affiliation: Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Andres
authors.initials: A
authors.lastname: Garcia-Granados
authors.affiliation: Departamento de Quimica Organica, Facultad de Ciencias, Universidad de Granada, Granada, Spain.
authors.forename: Andres
authors.initials: A
authors.lastname: Parra
mesh_terms: D002851:Chromatography, High Pressure Liquid; D005638:Fruit / Q000737:chemistry; D008872:Microwaves; D031658:Olea / Q000737:chemistry*; D053719:Tandem Mass Spectrometry; D014315:Triterpenes / Q000302:isolation & purification*
chemical_list: D014315:Triterpenes
keywords: maslinic acid; microwave-assisted extraction; oleanolic acid; olive skin