title: CYP1A1,CYP2E1andEPHX1polymorphisms in sporadic colorectal neoplasms.
abstract: OBJECTIVE
  To investigate the contribution of polymorphisms in theCYP1A1,CYP2E1andEPHX1genes on sporadic colorectal cancer (SCRC) risk.
  
  
  METHODS
  Six hundred forty-one individuals (227 patients with SCRC and 400 controls) were enrolled in the study. The variables analyzed were age, gender, tobacco and alcohol consumption, and clinical and histopathological tumor parameters. TheCYP1A1*2A,CYP1A1*2CCYP2E1*5B andCYP2E1*6 polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). TheEPHX1Tyr113His,EPHX1His139Arg andCYP1A1*2C polymorphisms were detected by real-time PCR. Chi-squared test and binary logistic regression were used in the statistical analysis. Haplotype analysis was conducted using the Haploview program, version 2.05.
  
  
  RESULTS
  Age over 62 years was a risk factor for SCRC development (OR = 7.54, 95%CI: 4.94-11.50,P< 0.01). Male individuals were less susceptible to SCRC (OR = 0.55, 95%CI: 0.35-0.85,P< 0.01). TheCYP2E1*5Bpolymorphism was associated with SCRC in the codominant (heterozygous genotype: OR = 2.66, 95%CI: 1.64-4.32,P< 0.01), dominant (OR = 2.82, 95%CI: 1.74-4.55,P< 0.01), overdominant (OR = 2.58, 95%CI: 1.59-4.19,P< 0.01), and log-additive models (OR = 2.84, 95%CI: 1.78-4.52,P< 0.01). TheCYP2E1*6polymorphism was associated with an increased SCRC risk in codominant (heterozygous genotype: OR = 2.81, 95%CI: 1.84-4.28,P< 0.01; homozygous polymorphic: OR = 7.32, 95%CI: 1.85-28.96,P< 0.01), dominant (OR = 2.97, 95%CI: 1.97-4.50,P< 0.01), recessive (OR = 5.26, 95%CI: 1.35-20.50,P= 0.016), overdominant (OR = 2.64, 95%CI: 1.74-4.01,P< 0.01), and log-additive models (OR = 2.78, 95%CI: 1.91-4.06,P< 0.01). The haplotype formed by the minor alleles of the CYP2E1*5B (C) and CYP2E1*6 (A) polymorphisms was associated with SCRC (P= 0.002). However, theCYP1A1*2A,CYP1A1*2C,EPHX1 Tyr113HisandEPHX1 His139Argpolymorphisms were not associated with SCRC.
  
  
  CONCLUSIONS
  In conclusion, the results demonstrated thatCYP2E1*5BandCYP2E1*6minor alleles play a role in the development of SCRC.
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Glaucia Maria M
authors.initials: GM
authors.lastname: Fernandes
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Anelise
authors.initials: A
authors.lastname: Russo
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Marcela Alcântara
authors.initials: MA
authors.lastname: Proença
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Nathalia Fernanda
authors.initials: NF
authors.lastname: Gazola
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Gabriela Helena
authors.initials: GH
authors.lastname: Rodrigues
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Patrícia Matos
authors.initials: PM
authors.lastname: Biselli-Chicote
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Ana Elizabete
authors.initials: AE
authors.lastname: Silva
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: João Gomes
authors.initials: JG
authors.lastname: Netinho
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Érika Cristina
authors.initials: ÉC
authors.lastname: Pavarino
authors.affiliation: Glaucia Maria M Fernandes, Anelise Russo, Nathalia Fernanda Gazola, Gabriela Helena Rodrigues, Patrícia Matos Biselli-Chicote, Érika Cristina Pavarino, Eny Maria Goloni-Bertollo, Genetics and Molecular Biology Research Unit - UPGEM, Department of Molecular Biology, São José do Rio Preto Medical School, FAMERP, São José do Rio Preto, SP 15090-000, Brazil.
authors.forename: Eny Maria
authors.initials: EM
authors.lastname: Goloni-Bertollo
mesh_terms: D000367:Age Factors; D001938:Brazil; D016022:Case-Control Studies; D015179:Colorectal Neoplasms / Q000235:genetics*; D019363:Cytochrome P-450 CYP1A1 / Q000235:genetics*; D019392:Cytochrome P-450 CYP2E1 / Q000235:genetics*; D004851:Epoxide Hydrolases / Q000235:genetics*; D005260:Female; D006801:Humans; D016015:Logistic Models; D008297:Male; D008875:Middle Aged; D016133:Polymerase Chain Reaction; D011110:Polymorphism, Genetic; D012150:Polymorphism, Restriction Fragment Length; D020641:Polymorphism, Single Nucleotide; D060888:Real-Time Polymerase Chain Reaction; D012307:Risk Factors; D012737:Sex Factors
chemical_list: D019392:Cytochrome P-450 CYP2E1; C569463:CYP1A1 protein, human; D019363:Cytochrome P-450 CYP1A1; D004851:Epoxide Hydrolases; C522330:EPHX1 protein, human
keywords: Colorectal neoplasms; Cytochrome P-450 CYP1A1; Cytochrome P-450 CYP2E1; Epoxide hydrolases 1; Single-nucleotide polymorphisms