title: A Polysaccharide from the Culinary-Medicinal Mushroom Pholiota nameko (Agaricomycetes) Inhibits the NF-κB Pathway in Dendritic Cells Through the TLR2 Receptor.
abstract: A polysaccharide purified from Pholiota nameko (PNPS-1) was found to have anticancer and anti-inflammatory activity. This study investigated the effect of PNPS-1 on the nuclear factor (NF)-κB signaling pathway of TLR2 small interfering RNA-silenced murine bone marrow-derived dendritic cells (BMDCs) and relevant mechanisms. The expression of messenger RNA of 4 NF-κB-related genes, including MyD88, IKBKB, RelA(p65), and CCL2, was determined by real-time polymerase chain reaction; the expression of the phenotype molecule intercellular adhesion molecule-1 (ICAM-1) by flow cytometry; the protein expression of IKKβ and p65 by Western blot; the production of p65 by enzyme-linked immunosorbent assay; and the expression of p65 by immunocytochemistry. The results showed that TLR2-specific small interfering RNA could effectively inhibit the decrease in the expression of MyD88, IKBKB, CCL2, p65, and ICAM-1 in BMDCs induced by PNPS-1, and thus the transcription inactivation of NF-κB, which obviously suggests that PNPS-1 could downregulate the NF-κB signaling pathway via the TLR2 receptor.
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, P. R. China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, P. R. China.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, P. R. China.
authors.forename: Fengling
authors.initials: F
authors.lastname: Wang
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, P. R. China.
authors.forename: Lihua
authors.initials: L
authors.lastname: Huai
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, P. R. China.
authors.forename: Ruixuan
authors.initials: R
authors.lastname: Zhu
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, P. R. China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Li
authors.affiliation: Tianjin Key Laboratory of Food Biotechnology, Faculty of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin, P. R. China.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Xu
mesh_terms: D000818:Animals; D015153:Blotting, Western; D003713:Dendritic Cells / Q000187:drug effects* / Q000276:immunology*; D004797:Enzyme-Linked Immunosorbent Assay; D005434:Flow Cytometry; D020869:Gene Expression Profiling; D007166:Immunosuppressive Agents / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D008810:Mice, Inbred C57BL; D016328:NF-kappa B / Q000037:antagonists & inhibitors*; D055368:Pholiota / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification* / Q000494:pharmacology; D060888:Real-Time Polymerase Chain Reaction; D051195:Toll-Like Receptor 2 / Q000819:agonists*
chemical_list: D007166:Immunosuppressive Agents; D016328:NF-kappa B; D011134:Polysaccharides; D051195:Toll-Like Receptor 2