title: ZnO films formed by atomic layer deposition as an optical biosensor platform for the detection of Grapevine virus A-type proteins.
abstract: Novel sensitive optical biosensor for determination of Grapevine virus A-type (GVA) proteins (GVA-antigens) has been designed. This biosensor was based on thin films of Zinc Oxide (ZnO) deposited by atomic layer deposition (ALD). The ZnO-based films have demonstrated favorable surface-structural properties for the direct immobilization of antibodies against GVA-antigens in order to form a biosensitive layer sensitive to GVA-antigens. The immobilization was confirmed by intensity changes in the main near band emission (NBE) peak of ZnO and by the formation of intense photoluminescence band, discovered in the visible range around 425nm, caused by the immobilized proteins. The GVA-antigen detection was performed by the evaluation of changes and behavior of a corresponding luminescence band. The sensitivity of as-formed label-free biosensor towards the GVA-antigens was determined in the range from 1pg/ml to 10ng/ml; in addition, the selectivity of biosensor was evaluated.
authors.affiliation: Experimental Physics Department, Odesa National I. I. Mechnikov University, 42, Pastera, 65023, Odesa, Ukraine. Electronic address: alla_teresc@onu.edu.ua.
authors.forename: Alla
authors.initials: A
authors.lastname: Tereshchenko
authors.affiliation: Experimental Physics Department, Odesa National I. I. Mechnikov University, 42, Pastera, 65023, Odesa, Ukraine; Institut Européen des Membranes IEMM, ENSCM UM CNRS UMR5635, Place Eugène Bataillon, F-34095 Montpellier Cedex 5, France.
authors.forename: Viktoriia
authors.initials: V
authors.lastname: Fedorenko
authors.affiliation: Experimental Physics Department, Odesa National I. I. Mechnikov University, 42, Pastera, 65023, Odesa, Ukraine.
authors.forename: Valentyn
authors.initials: V
authors.lastname: Smyntyna
authors.affiliation: Department of Microbiology, Virology and Biotechnology, Odessa National I. I. Mechnikov University, 2, Shampanskiy Lane, 65000, Odesa, Ukraine.
authors.forename: Igor
authors.initials: I
authors.lastname: Konup
authors.affiliation: National Scientific Centre "Institute of Viticulture and Wine Making Named After V. Ye. Tairov", 27, 40 Let Pobedy Str., 65496, Odesa, Ukraine.
authors.forename: Anastasiya
authors.initials: A
authors.lastname: Konup
authors.affiliation: Department of Physics, Chemistry and Biology, Linköping University, 58183 Linköping, Sweden.
authors.forename: Martin
authors.initials: M
authors.lastname: Eriksson
authors.affiliation: Department of Physics, Chemistry and Biology, Linköping University, 58183 Linköping, Sweden.
authors.forename: Rositsa
authors.initials: R
authors.lastname: Yakimova
authors.affiliation: Department of Physical Chemistry, Vilnius University, Naugarduko Str. 24, LT-03225 Vilnius, Lithuania.
authors.forename: Arunas
authors.initials: A
authors.lastname: Ramanavicius
authors.affiliation: Institut Européen des Membranes IEMM, ENSCM UM CNRS UMR5635, Place Eugène Bataillon, F-34095 Montpellier Cedex 5, France.
authors.forename: Sebastien
authors.initials: S
authors.lastname: Balme
authors.affiliation: Institut Européen des Membranes IEMM, ENSCM UM CNRS UMR5635, Place Eugène Bataillon, F-34095 Montpellier Cedex 5, France.
authors.forename: Mikhael
authors.initials: M
authors.lastname: Bechelany
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry*; D000956:Antigens, Viral / Q000032:analysis*; D015374:Biosensing Techniques / Q000379:methods; D057230:Limit of Detection; D049449:Luminescence; D008163:Luminescent Measurements / Q000379:methods*; D010935:Plant Diseases / Q000821:virology; D010942:Plant Viruses / Q000302:isolation & purification*; D013499:Surface Properties; D014764:Viral Proteins / Q000032:analysis*; D027843:Vitis / Q000821:virology*; D015034:Zinc Oxide / Q000737:chemistry*
chemical_list: D055910:Antibodies, Immobilized; D000956:Antigens, Viral; D014764:Viral Proteins; D015034:Zinc Oxide
keywords: Atomic layer deposition; Grapevine virus A-type; Optical biosensor; Photoluminescence; ZnO