title: Whole cell-SELEX of aptamers with a tyrosine-like side chain against live bacteria.
abstract: In an effort to expand the binding and recognition capabilities of aptamers, a nucleoside triphosphate modified with a phenol that mimics the side chain of tyrosine was used in the selection of DNA aptamers against live bacteria. Of multiple modified aptamers that were isolated against Escherichia coli DH5α cells, one aptamer displays high selectivity and affinity for the target cells and is greatly enriched for phenol-modified dU nucleotides (dUy, 47.5%). When the same sequences are synthesized with TTP, no binding is observed. Taken together, these findings highlight the value of using modified nucleotide triphosphates in aptamer selections and portends success in SELEX against an array of whole cells as targets.
authors.affiliation: Chemistry Department, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada. dperrin@chem.ubc.ca.
authors.forename: Marleen
authors.initials: M
authors.lastname: Renders
authors.affiliation: Chemistry Department, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada. dperrin@chem.ubc.ca.
authors.forename: Emily
authors.initials: E
authors.lastname: Miller
authors.affiliation: Chemistry Department, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada. dperrin@chem.ubc.ca.
authors.forename: Curtis H
authors.initials: CH
authors.lastname: Lam
authors.affiliation: Chemistry Department, University of British Columbia, 2036 Main Mall, Vancouver, BC V6T 1Z1, Canada. dperrin@chem.ubc.ca.
authors.forename: David M
authors.identifier: http://orcid.org/0000-0001-8342-6346
authors.initials: DM
authors.lastname: Perrin
mesh_terms: D004305:Dose-Response Relationship, Drug; D004926:Escherichia coli / Q000166:cytology / Q000187:drug effects* / Q000235:genetics; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D019800:Phenol / Q000737:chemistry / Q000494:pharmacology*; D052156:SELEX Aptamer Technique; D013329:Structure-Activity Relationship; D014443:Tyrosine / Q000031:analogs & derivatives / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D019800:Phenol; D014443:Tyrosine