title: Olive oil polyphenols: A quantitative method by high-performance liquid-chromatography-diode-array detection for their determination and the assessment of the related health claim.
abstract: In order to assess if an extra virgin olive oil (EVOO) can be acknowledged with the health claim related to olive oil polyphenols (Reg. EU n.432/2012), a new method to quantify these species in EVOO, by means of liquid-liquid extraction followed by HPLC-DAD/MS/MS of the hydroalcoholic extract, has been developed and validated. Different extraction procedures, different types of reverse-phase analytical columns (Synergi Polar, Spherisorb ODS2 and Kinetex) and eluents have been tested. The chromatographic column Synergi Polar (250×4.6mm, 4μm), never used before in this kind of application, provided the best results, with water and methanol/isopropanol (9/1) as eluents. The method allows the quantification of the phenolic alcohols tyrosol and hydroxytyrosol, the phenolic acids vanillic, p-coumaric and ferulic acids, secoiridoids derivatives, the lignans, pinoresinol and acetoxypinoresinol and the flavonoids luteolin and apigenin. The new method has been applied to 20 commercial EVOOs belonging to two different price range categories (3.78-5.80 euros/L and 9.5-25.80 euros/L) and 5 olive oils. The obtained results highlight that acetoxypinoresinol, ferulic acid, vanillic acid and the total non secoiridoid phenolic substances resulted to be significantly higher in HEVOOs than in LEVOOs (P=0.0026, 0.0217, 0.0092, 0.0003 respectively). For most of the samples analysed there is excellent agreement between the results obtained by applying the HPLC method adopted by the International Olive Council and the results obtained by applying the presented HPLC method. Results obtained by HPLC methods have been also compared with the ones obtained by the colorimetric Folin-Ciocalteu method.
authors.affiliation: HPLC-MS Laboratory, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
authors.forename: Massimo
authors.initials: M
authors.lastname: Ricciutelli
authors.affiliation: School of Science and Technology, Chemistry Division, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
authors.forename: Shara
authors.initials: S
authors.lastname: Marconi
authors.affiliation: School of Science and Technology, Chemistry Division, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
authors.forename: Maria Chiara
authors.initials: MC
authors.lastname: Boarelli
authors.affiliation: School of Pharmacy, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Caprioli
authors.affiliation: School of Pharmacy, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
authors.forename: Gianni
authors.initials: G
authors.lastname: Sagratini
authors.affiliation: School of Science and Technology, Chemistry Division, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Ballini
authors.affiliation: School of Science and Technology, Chemistry Division, University of Camerino, V. S. Agostino 1, I-62032 Camerino, MC, Italy. Electronic address: dennis.fiorini@unicam.it.
authors.forename: Dennis
authors.initials: D
authors.lastname: Fiorini
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation* / Q000379:methods*; D006262:Health; D031658:Olea / Q000737:chemistry; D000069463:Olive Oil / Q000737:chemistry*; D010636:Phenols / Q000032:analysis; D059808:Polyphenols / Q000032:analysis* / Q000302:isolation & purification; D015203:Reproducibility of Results; D013053:Spectrophotometry; D053719:Tandem Mass Spectrometry
chemical_list: D000069463:Olive Oil; D010636:Phenols; D059808:Polyphenols
keywords: Extra virgin olive oil; Folin-Ciocalteu; HPLC-DAD; Health claim; Polyphenols; Quality markers