title: The combination of db-cAMP and ChABC with poly(propylene carbonate) microfibers promote axonal regenerative sprouting and functional recovery after spinal cord hemisection injury.
abstract: This study describes the use of poly(propylene carbonate) (PPC) electrospun microfibres impregnated with a combination of dibutyryl cyclic adenosine monophosphate (db-cAMP) and chondroitinase ABC (ChABC) in the treatment of right-side hemisected spinal cord injury (SCI). Release of db-cAMP and/or ChABC from the microfibres was assessed in vitro using high-performance liquid chromatography (HPLC). Drug-impregnated microfibres were implanted into the hemisected thoracic spinal cord of rats, and treatment was evaluated using functional recovery examinations and immunohistochemistry. Our results demonstrated that the microfibres containing db-cAMP and/or ChABC displayed a stable and prolonged release of each agent. Sustained delivery of db-cAMP and/or ChABC was found to promote axonal regenerative sprouting, functional recovery, and reduced glial scar formation when compared to untreated control animals. The combination of both db-cAMP and ChABC was determined to be more effective than using either drug alone in the treatment of SCI. These findings demonstrate the feasibility of using PPC electrospun microfibres for multi-drug combination therapy in SCI.
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Tongliang
authors.initials: T
authors.lastname: Xia
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Bin
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Shilei
authors.initials: S
authors.lastname: Ni
authors.affiliation: Jiyang County People's Hospital of Shandong Provence, 9# Xinyuan Street, Jinyang County, Jinan, 251400, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Gao
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Jiangang
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China; Department of Biomedicine, University of Bergen, Jonas Lies Vei 91, 5009 Bergen, Norway.
authors.forename: Jian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Anjing
authors.initials: A
authors.lastname: Chen
authors.affiliation: Department of Neurology, Qilu Hospital of Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Shaowei
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Benlin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Gang
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China.
authors.forename: Shugan
authors.initials: S
authors.lastname: Zhu
authors.affiliation: Department of Neurosurgery, Qilu Hospital of Shandong University and Brain Science Research Institute, Shandong University, 107# Wenhua Xi Road, Jinan, 250012, PR China. Electronic address: lixg@sdu.edu.cn.
authors.forename: Xingang
authors.initials: X
authors.lastname: Li
mesh_terms: D000818:Animals; D001369:Axons / Q000187:drug effects*; D019765:Chondroitin ABC Lyase / Q000502:physiology*; D000242:Cyclic AMP / Q000494:pharmacology*; D005260:Female; D011407:Propane / Q000031:analogs & derivatives* / Q000494:pharmacology; D051381:Rats; D017208:Rats, Wistar; D020127:Recovery of Function / Q000187:drug effects*; D013116:Spinal Cord / Q000187:drug effects; D013119:Spinal Cord Injuries / Q000188:drug therapy*
chemical_list: C045990:propylene carbonate; D000242:Cyclic AMP; D019765:Chondroitin ABC Lyase; D011407:Propane
keywords: ChABC; Electrospinning; PPC; SCI; db-cAMP