title: Overexpression of tomato SlGGP-LIKE gene improves tobacco tolerance to methyl viologen-mediated oxidative stress.
abstract: Ascorbate (AsA) is very important in scavenging reactive oxygen species in plants. AsA can reduce photoinhibition by xanthophyll cycle to dissipate excess excitation energy. GGP is an important enzyme in AsA biosynthesis pathway in higher plants. In this study, we cloned a gene, SlGGP-LIKE, that has the same function but different sequence compared with SlGGP. The function of SlGGP-LIKE gene in response to oxidative stress was investigated using transgenic tobacco plants overexpressed SlGGP-LIKE under methyl viologen treatment. After oxidative stress treatment, transgenic tobacco lines exhibited higher levels of reduced AsA content and APX activity than WT plants. Under oxidative stress, transgenic tobacco plants accumulated less ROS and exhibited lower degrees of REC and MDA. Consequently, relatively higher levels of Pn, Fv/Fm, de-epoxidation status of xanthophyll cycle and D1 protein were maintained in transgenic tobacco plants. Hence, overexpression of SlGGP-LIKE gene enhances AsA biosynthesis and can alleviate the photoinhibition of PSII under oxidative stress.
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Dong-Yue
authors.initials: DY
authors.lastname: Yang
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Na-Na
authors.initials: NN
authors.lastname: Ma
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Kun-Yang
authors.initials: KY
authors.lastname: Zhuang
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Shao-Bo
authors.initials: SB
authors.lastname: Zhu
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China.
authors.forename: Zhong-Ming
authors.initials: ZM
authors.lastname: Liu
authors.affiliation: College of Life Science, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, PR China. Electronic address: xhyang@sdau.edu.cn.
authors.forename: Xing-Hong
authors.initials: XH
authors.lastname: Yang
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics*; D000595:Amino Acid Sequence; D060387:Ascorbate Peroxidases / Q000378:metabolism; D001205:Ascorbic Acid / Q000378:metabolism; D018506:Gene Expression Regulation, Plant / Q000187:drug effects; D017343:Genes, Plant; D006861:Hydrogen Peroxide / Q000378:metabolism; D008027:Light; D018551:Solanum lycopersicum / Q000235:genetics*; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress / Q000187:drug effects*; D010269:Paraquat / Q000633:toxicity*; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D021381:Protein Transport / Q000187:drug effects; D016415:Sequence Alignment; D013347:Subcellular Fractions / Q000378:metabolism; D013481:Superoxides / Q000378:metabolism; D014026:Nicotiana / Q000187:drug effects / Q000235:genetics / Q000502:physiology* / Q000528:radiation effects; D024341:Xanthophylls / Q000378:metabolism; D065146:Zeaxanthins / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D024341:Xanthophylls; D065146:Zeaxanthins; D013481:Superoxides; D008315:Malondialdehyde; D006861:Hydrogen Peroxide; D060387:Ascorbate Peroxidases; D010269:Paraquat; D001205:Ascorbic Acid
keywords: AsA; GGP; Methyl viologen; ROS; Tobacco; Violaxanthin de-epoxidase