title: Perineural dexmedetomidine effects on sciatic nerve in rat.
abstract: The present study was designed to test the hypothesis that high dose dexmedetomidine would increase the duration of antinociception to a thermal stimulus in a rat model of sciatic nerve blockade without causing nerve damage. The rats were anesthetized with isoflurane. After electromyography (EMG) recordings, right sciatic nerves were explored and perineural injections were delivered: Group D (n=7), 40μgμgkg-1dexmedetomidine administration, Group II (n=6), (0.2mL) saline administration, Group III (n=2), only surgically exploration of the right sciatic nevre. Time to paw withdrawal latency (PAW) to a thermal stimulus for both paws and an assessment of motor function were measured every 30min after the nerve block until a return to baseline. The compound muscle action potential (CMAP) of right and left sciatic nerves were recorded 10 times per each nerve once more after perineural injections at 14 day. After EMG recordings, right and the part of left sciatic nerve were excised at a length of at minimum 15mm for histopathological examination. Comparison of right/left CMAP amplitude ratios before and 14 days after the procedure showed a statistically significant difference (p=0.000). There were no differences in perineural inflammation between the Group D, Group S, and Group E at 14 days.
authors.affiliation: Bagcilar Training and Research Hospital, Istanbul, Turkey. Electronic address: akyektas722000@yahoo.co.uk.
authors.forename: Abdulkadir
authors.initials: A
authors.lastname: Yektaş
authors.affiliation: Bakırköy Dr. Sadikonuk eğitim ve araştırma hastanesi, İstanbul, Turkey.
authors.forename: Murat
authors.initials: M
authors.lastname: Çabalar
authors.affiliation: Bakırköy Dr. Sadikonuk eğitim ve araştırma hastanesi, İstanbul, Turkey.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Sar
authors.affiliation: Bagcilar Egitim ve Arastirma Hastanesi, Istanbul, Turkey.
authors.forename: Ayşin
authors.initials: A
authors.lastname: Alagöl
authors.affiliation: Bagcilar Egitim ve Arastirma Hastanesi, Istanbul, Turkey.
authors.forename: Duygu Sultan
authors.initials: DS
authors.lastname: Çelik
authors.affiliation: Bakırköy Dr. Sadikonuk eğitim ve araştırma hastanesi, İstanbul, Turkey.
authors.forename: Vildan
authors.initials: V
authors.lastname: Yayla
authors.affiliation: Bagcilar Egitim ve Arastirma Hastanesi, Istanbul, Turkey.
authors.forename: Deniz
authors.initials: D
authors.lastname: Tolga
mesh_terms: D018712:Analgesics, Non-Narcotic / Q000494:pharmacology*; D000704:Analysis of Variance; D000818:Animals; D020927:Dexmedetomidine / Q000494:pharmacology*; D004558:Electric Stimulation; D004576:Electromyography; D035002:Lower Extremity; D008297:Male; D009407:Nerve Block / Q000379:methods; D009443:Neuritis / Q000139:chemically induced; D017207:Rats, Sprague-Dawley; D011930:Reaction Time; D012584:Sciatic Nerve / Q000187:drug effects*
chemical_list: D018712:Analgesics, Non-Narcotic; D020927:Dexmedetomidine
keywords: Analgesia meter; Dexmedetomidina; Dexmedetomidine; Electromyography; Eletromiografia; Medidor de analgesia; Nervo ciático; Paw withdrawal latency testing; Sciatic nerve; Testes de latência de retirada da pata