title: Comparison of cardiac stem cell sheets detached by Versene solution and from thermoresponsive dishes reveals similar properties of constructs.
abstract: Cell sheets (CS) from c-kit+ cardiac stem cell (CSC) hold a potential for application in regenerative medicine. However, manufacture of CS may require thermoresponsive dishes, which increases cost and puts one in dependence on specific materials. Alternative approaches were established recently and we conducted a short study to compare approaches for detachment of CS from c-kit+ CSC. Our in-house developed method using chelation by Versene solution was compared to UpCell™ thermoresponsive plates in terms of CSC proliferation, viability, gap junction formation and engraftment in a model of myocardial infarction. Use of Versene solution instead of thermoresponsive dishes resulted in comparable CS thickness (approximately 100mcm), cell proliferation rate and no signs of apoptosis detected in both types of constructs. However, we observed a minor reduction of gap junction count in Versene-treated CS. At day 30 after delivery to infarcted myocardium both types of CS retained at the site of transplantation and contained comparable amounts of proliferating cells indicating engraftment. Thus, we may conclude that detachment of CS from c-kit+ CSC using Versene solution followed by mechanical treatment is an alternative to thermoresponsive plates allowing use of routinely available materials to generate constructs for cardiac repair.
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation.
authors.forename: Konstantin V
authors.initials: KV
authors.lastname: Dergilev
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation; Lomonosov Moscow State University, Medical Research and Education Centre, Institute of Regenerative Medicine, Laboratory of gene and cell therapy, 119192, Moscow, Russian Federation. Electronic address: pmakarevich@mc.msu.ru.
authors.forename: Pavel I
authors.initials: PI
authors.lastname: Makarevich
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation.
authors.forename: Zoya I
authors.initials: ZI
authors.lastname: Tsokolaeva
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation.
authors.forename: Maria A
authors.initials: MA
authors.lastname: Boldyreva
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation.
authors.forename: Irina B
authors.initials: IB
authors.lastname: Beloglazova
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation.
authors.forename: Ekaterina S
authors.initials: ES
authors.lastname: Zubkova
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation.
authors.forename: Mikhail Yu
authors.initials: MY
authors.lastname: Menshikov
authors.affiliation: Russian Cardiology Research and Production Complex, Laboratory of Angiogenesis, 121552, Moscow, Russian Federation; Lomonosov Moscow State University, Faculty of Medicine, Laboratory of gene and cell technologies, 119192, Moscow, Russian Federation.
authors.forename: Yelena V
authors.initials: YV
authors.lastname: Parfyonova
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000235:genetics; D018929:Cell Culture Techniques / Q000379:methods*; D049109:Cell Proliferation / Q000187:drug effects; D002469:Cell Separation / Q000379:methods*; D002470:Cell Survival / Q000187:drug effects; D004492:Edetic Acid / Q000494:pharmacology; D017629:Gap Junctions / Q000187:drug effects; D006801:Humans; D009203:Myocardial Infarction / Q000473:pathology / Q000628:therapy*; D009206:Myocardium / Q000473:pathology; D051381:Rats; D044968:Regenerative Medicine; D033581:Stem Cell Transplantation; D013234:Stem Cells / Q000187:drug effects
chemical_list: D004492:Edetic Acid
keywords: Cardiac stem cell; Cell sheet; Myocardial infarction; Thermoresponsive dish; Tissue engineering