title: Labeling of adipose-derived stem cells with quantum dots provides stable and long-term fluorescent signal for ex vivo cell tracking.
abstract: Stem cells derived from adipose tissue (ADSC) have been used in cell therapy as an alternative to treat chronic and degenerative diseases. Using biomedical and image trials to track the cells when infused in the target tissue is essential to control cell migration and adhesion. The objective of the present study was to label and assess the adhesion of goat adipose tissue-derived stem cells (g-ADSC) after cell infusion in animal models by tracking luminescent intracytoplasmatic nanocrystals. The cells were labeled by using Qdots. The g-ADSCs infused with nanocrystal were prepared either fresh or fixed and further visualized under a fluorescence microscope. The labeled cells were infused in the goat mammary glands and mouse testicles and kidneys via tail vein injection. Thirty days after cell infusion, biopsy was carried out for analyses. The g-ADSC cultures were presented with high cellularity and fibroblast morphology, even after infusion of the nanocrystals. It was possible, by processing in paraffin and under fluorescence microscopy, demonstrating the success of the labeling in the long term. Freezing mammary gland biopsies in liquid NO2did not alter the quality of labeling with Qdots. Therefore, g-ADSCs can be labeled with intracytoplasmatic nanocrystals (Qdots) enabling their in vitro and ex vivo tracking.
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Clautina R M
authors.initials: CRM
authors.lastname: Costa
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Matheus L T
authors.initials: MLT
authors.lastname: Feitosa
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Dayseanny O
authors.initials: DO
authors.lastname: Bezerra
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Yulla K P
authors.initials: YKP
authors.lastname: Carvalho
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Rodrigo F G
authors.initials: RFG
authors.lastname: Olivindo
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Pablo B
authors.initials: PB
authors.lastname: Fernando
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Gustavo C
authors.initials: GC
authors.lastname: Silva
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Mirna L G
authors.initials: MLG
authors.lastname: Silva
authors.affiliation: Veterinary Medicine Department, Faculty of Animal Science and Food Engineering (FZEA), University of São Paulo (USP), São Paulo, Brazil.
authors.forename: Carlos E
authors.initials: CE
authors.lastname: Ambrósio
authors.affiliation: Morphology Department, Federal University of Piauí, Teresina, Piauí, Brazil.
authors.forename: Airton M
authors.initials: AM
authors.lastname: Conde Júnior
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Napoleão M
authors.initials: NM
authors.lastname: Argolo Neto
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil.
authors.forename: Laís M
authors.initials: LM
authors.lastname: Costa Silva
authors.affiliation: Integrated Nucleus of Morphology and Stem Cell Research (NUPCelt), Federal University of Piauí (UFPI), Teresina, Piauí, Brazil. mcelina@ufpi.edu.br.
authors.forename: Maria A M
authors.initials: MAM
authors.lastname: Carvalho
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D000818:Animals; D058948:Cell Tracking / Q000379:methods*; D005260:Female; D005453:Fluorescence; D006041:Goats; D008321:Mammary Glands, Animal / Q000166:cytology; D051379:Mice; D045663:Quantum Dots / Q000378:metabolism*; D013194:Staining and Labeling; D013234:Stem Cells / Q000378:metabolism*
keywords: Adipose-derived stem cells; Cell tracking; Qdots; Stem cells