title: The Genome of the Trinidadian Guppy, Poecilia reticulata, and Variation in the Guanapo Population.
abstract: For over a century, the live bearing guppy, Poecilia reticulata, has been used to study sexual selection as well as local adaptation. Natural guppy populations differ in many traits that are of intuitively adaptive significance such as ornamentation, age at maturity, brood size and body shape. Water depth, light supply, food resources and predation regime shape these traits, and barrier waterfalls often separate contrasting environments in the same river. We have assembled and annotated the genome of an inbred single female from a high-predation site in the Guanapo drainage. The final assembly comprises 731.6 Mb with a scaffold N50 of 5.3 MB. Scaffolds were mapped to linkage groups, placing 95% of the genome assembly on the 22 autosomes and the X-chromosome. To investigate genetic variation in the population used for the genome assembly, we sequenced 10 wild caught male individuals. The identified 5 million SNPs correspond to an average nucleotide diversity (π) of 0.0025. The genome assembly and SNP map provide a rich resource for investigating adaptation to different predation regimes. In addition, comparisons with the genomes of other Poeciliid species, which differ greatly in mechanisms of sex determination and maternal resource allocation, as well as comparisons to other teleost genera can begin to reveal how live bearing evolved in teleost fish.
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.|Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Plön, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Künstner
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Margarete
authors.initials: M
authors.lastname: Hoffmann
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Bonnie A
authors.initials: BA
authors.lastname: Fraser
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Verena A
authors.initials: VA
authors.lastname: Kottler
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Eshita
authors.initials: E
authors.lastname: Sharma
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Detlef
authors.initials: D
authors.lastname: Weigel
authors.affiliation: Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany.
authors.forename: Christine
authors.initials: C
authors.lastname: Dreyer
mesh_terms: D000222:Adaptation, Physiological; D000818:Animals; D004252:DNA Mutational Analysis; D005260:Female; D023281:Genomics; D008297:Male; D058977:Molecular Sequence Annotation; D011036:Poecilia / Q000235:genetics* / Q000502:physiology; D020641:Polymorphism, Single Nucleotide; D012730:Sex Chromosomes / Q000235:genetics; D026801:Synteny