title: Identification and expression profiling of pheromone biosynthesis activating neuropeptide in Chlumetia transversa (Walker).
abstract: Insect neuropeptides (NPs) in the pyrokinin/pheromone biosynthesis-activating neuropeptide (PBAN) family are actively involved in many essential endocrine functions. These peptides are potential targets in the search for novel insect control agents. This is the first report on the cloning and sequence determination of Chlumetia transversa (Walker) PBAN (Ct-PBAN) using rapid amplification of cDNA ends. The open reading frame of Ct-PBAN was 588bp in length and encoded 195 amino acids, which were assembled into five putative neuropeptides (diapause hormone homolog, α-neuropeptide, β-neuropeptide, PBAN, and γ-neuropeptide). These peptides were amidated at C-terminus and shared the conserved pentapeptide motif FXPR (or K) L. Moreover, Ct-PBAN had high homology to PBANs in Helicoverpa zea (84.1%), Helicoverpa armigera (83.5%), Helicoverpa assulta (83%), and Heliothis virescens (82.6%). Phylogenetic analysis showed that Ct-PBAN was closely related to its orthologs in the family Noctuidae. In addition, real-time quantitative polymerase chain reaction assays showed that the expression of Ct-PBAN peaked in the female head and was also detected at high levels in 1-d-old adults. These results suggested that Ct-PBAN is associated with sex pheromone biosynthesis in female C. transversa and could be used for developing C. transversa control systems based on molecular techniques.
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China. Electronic address: weihui@faas.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wei
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; College of Plant Protection, Fujian Agriculture and Forestry University, 15 Shangxia Dian Road, Fuzhou 350002, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Chang
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Lin
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China.
authors.forename: Yixin
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China.
authors.forename: Houjun
authors.initials: H
authors.lastname: Tian
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Institute of Plant Protection, ', 247 Wusi Road, Fuzhou 350003, China; Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, 247 Wusi Road, Fuzhou 350003, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Fishery college, Jimei University, 43 Yindou Road, Xiamen 361021, China.
authors.forename: Hongjiao
authors.initials: H
authors.lastname: Cai
authors.affiliation: College of Plant Protection, Fujian Agriculture and Forestry University, 15 Shangxia Dian Road, Fuzhou 350002, China. Electronic address: guxiaojun1@163.com.
authors.forename: Xiaojun
authors.initials: X
authors.lastname: Gu
authors.affiliation: Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.
authors.forename: Kadarkarai
authors.initials: K
authors.lastname: Murugan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D018076:DNA, Complementary / Q000235:genetics; D005260:Female; D020869:Gene Expression Profiling; D019476:Insect Proteins / Q000235:genetics*; D008297:Male; D009036:Moths / Q000235:genetics*; D009479:Neuropeptides / Q000235:genetics*; D010802:Phylogeny; D017422:Sequence Analysis, DNA
chemical_list: D018076:DNA, Complementary; D019476:Insect Proteins; D009479:Neuropeptides; C079709:pheromone biosynthesis activating neuropeptide, Helicoverpa zea
keywords: Diapause hormone; Endocrine regulation; Lepidoptera; Moth pheromone; PBAN