title: Platelet responses to agonists in a cohort of highly characterised platelet donors are consistent over time.
abstract: OBJECTIVE
  Platelet function shows significant inheritance that is at least partially genetically controlled. There is also evidence that the platelet response is stable over time, but there are few studies that have assessed consistency of platelet function over months and years. We aimed to measure platelet function in platelet donors over time in individuals selected from a cohort of 956 donors whose platelet function had been previously characterised.
  
  
  METHODS
  Platelet function was assessed by flow cytometry, measuring fibrinogen binding and P-selectin expression after stimulation with either cross-linked collagen-related peptide or adenosine 5'-diphosphate. Eighty-nine donors from the Cambridge Platelet Function Cohort whose platelet responses were initially within the lower or upper decile of reactivity were retested between 4 months and five and a half years later.
  
  
  RESULTS
  There was moderate-to-high correlation between the initial and repeat platelet function results for all assays (P ≤ 0·007, r20·2961-0·7625); furthermore, the range of results observed in the initial low and high responder groups remained significantly different at the time of the second test (P ≤ 0·0005).
  
  
  CONCLUSIONS
  Platelet function remains consistent over time. This implies that this potential influence on quality of donated platelet concentrates will remain essentially constant for a given donor.
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.|Department of Haematology, University of Cambridge, Cambridge, UK.
authors.forename: S F
authors.initials: SF
authors.lastname: Garner
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.|Department of Haematology, University of Cambridge, Cambridge, UK.
authors.forename: A
authors.initials: A
authors.lastname: Furnell
authors.affiliation: Pragmatic Clinical Trials Unit, Queen Mary University of London, London, UK.
authors.forename: B C
authors.initials: BC
authors.lastname: Kahan
authors.affiliation: Institute for Cardiovascular and Metabolic Research, University of Reading, Reading, UK.
authors.forename: C I
authors.initials: CI
authors.lastname: Jones
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.|Department of Haematology, University of Cambridge, Cambridge, UK.
authors.forename: A
authors.initials: A
authors.lastname: Attwood
authors.affiliation: Institute of Inflammation and Ageing, Queen Elizabeth Hospital, University of Birmingham, Birmingham, UK.
authors.forename: P
authors.initials: P
authors.lastname: Harrison
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.|Department of Haematology, University of Cambridge, Cambridge, UK.
authors.forename: A M
authors.initials: AM
authors.lastname: Kelly
authors.affiliation: Department of Cardiovascular Sciences, University of Leicester, Leicester, UK.|NIHR Cardiovascular Biomedical Research Unit, Glenfield Hospital, Leicester, UK.
authors.forename: A H
authors.initials: AH
authors.lastname: Goodall
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.|Department of Haematology, University of Cambridge, Cambridge, UK.
authors.forename: R
authors.initials: R
authors.lastname: Cardigan
authors.affiliation: NHS Blood and Transplant, Cambridge, UK.|Department of Haematology, University of Cambridge, Cambridge, UK.|Wellcome Trust Sanger Institute, Cambridge, UK.
authors.forename: W H
authors.initials: WH
authors.lastname: Ouwehand
mesh_terms: D000244:Adenosine Diphosphate / Q000032:analysis; D000328:Adult; D001782:Blood Donors; D001792:Blood Platelets / Q000166:cytology / Q000378:metabolism*; D002352:Carrier Proteins / Q000378:metabolism; D015331:Cohort Studies; D005260:Female; D005340:Fibrinogen / Q000737:chemistry / Q000378:metabolism; D005434:Flow Cytometry; D006801:Humans; D008297:Male; D008875:Middle Aged; D019007:P-Selectin / Q000378:metabolism; D010455:Peptides / Q000378:metabolism; D015539:Platelet Activation / Q000502:physiology*; D010979:Platelet Function Tests; D011485:Protein Binding
chemical_list: D002352:Carrier Proteins; D019007:P-Selectin; D010455:Peptides; C435908:collagen-related peptide; D000244:Adenosine Diphosphate; D005340:Fibrinogen
keywords: donors; platelet components; platelet function