title: Catalase-Modulated Heterogeneous Fenton Reaction for Selective Cancer Cell Eradication: SnFe2O4Nanocrystals as an Effective Reagent for Treating Lung Cancer Cells.
abstract: Heterogeneous Fenton reactions have been proven to be an effective and promising selective cancer cell treatment method. The key working mechanism for this method to achieve the critical therapeutic selectivity however remains unclear. In this study, we proposed and demonstrated for the first time the critical role played by catalase in realizing the therapeutic selectivity for the heterogeneous Fenton reaction-driven cancer cell treatment. The heterogeneous Fenton reaction, with the lattice ferric ions of the solid catalyst capable of converting H2O2to highly reactive hydroxyl radicals, can effectively eradicate cancer cells. In this study, SnFe2O4nanocrystals, a recently discovered outstanding heterogeneous Fenton catalyst, were applied for selective killing of lung cancer cells. The SnFe2O4nanocrystals, internalized into the cancer cells, can effectively convert endogenous H2O2into highly reactive hydroxyl radicals to invoke an intensive cytotoxic effect on the cancer cells. On the other hand, catalase, present at a significantly higher concentration in normal cells than in cancer cells, remarkably can impede the apoptotic cell death induced by the internalized SnFe2O4nanocrystals. According to the results obtained from the in vitro cytotoxicity study, the relevant oxidative attacks were effectively suppressed by the presence of normal physiological levels of catalase. The SnFe2O4nanocrystals were thus proved to effect apoptotic cancer cell death through the heterogeneous Fenton reaction and were benign to cells possessing normal physiological levels of catalase. The catalase modulation of the involved heterogeneous Fenton reaction plays the key role in achieving selective cancer cell eradication for the heterogeneous Fenton reaction-driven cancer cell treatment.
authors.affiliation: Technology Research Development Department, Plastics Industry Development Center , Taichung 40768, Taiwan ( ROC ).
authors.forename: Kuan-Ting
authors.initials: KT
authors.lastname: Lee
authors.affiliation: Department of Neurosurgery and ⬡Center for Advanced Molecular Imaging and Translation, Chang Gung Memorial Hospital , Tao-Yuan 33302, Taiwan ( ROC ).
authors.forename: Yu-Jen
authors.initials: YJ
authors.lastname: Lu
authors.forename: Fwu-Long
authors.initials: FL
authors.lastname: Mi
authors.forename: Thierry
authors.initials: T
authors.lastname: Burnouf
authors.affiliation: Technology Research Development Department, Plastics Industry Development Center , Taichung 40768, Taiwan ( ROC ).
authors.forename: Yi-Ting
authors.initials: YT
authors.lastname: Wei
authors.forename: Shao-Chieh
authors.initials: SC
authors.lastname: Chiu
authors.forename: Er-Yuan
authors.identifier: http://orcid.org/0000-0002-1593-4573
authors.initials: EY
authors.lastname: Chuang
authors.affiliation: Department of Chemical Engineering, National Tsing Hua University , Hsinchu 30013, Taiwan ( ROC ).
authors.forename: Shih-Yuan
authors.initials: SY
authors.lastname: Lu
mesh_terms: D002374:Catalase / Q000378:metabolism*; D005290:Ferric Compounds; D006801:Humans; D006861:Hydrogen Peroxide; D017665:Hydroxyl Radical; D008175:Lung Neoplasms; D053768:Metal Nanoparticles; D014001:Tin
chemical_list: D005290:Ferric Compounds; D017665:Hydroxyl Radical; D014001:Tin; D006861:Hydrogen Peroxide; D002374:Catalase
keywords: SnFe2O4; catalase modulation; heterogeneous Fenton reaction; hydrogen peroxide; hydroxyl radicals; lung cancer cell; selective cancer therapy