title: Active Antibacterial and Antifouling Surface Coating via a Facile One-Step Enzymatic Cross-Linking.
abstract: Prevention of microbial contamination of surfaces is one of the biggest challenges for biomedical applications. Establishing a stable, easily produced, highly antibacterial surface coating offers an efficient solution but remains a technical difficulty. Here, we report on a new approach to create an in situ hydrogel film-coating on glass surfaces made by enzymatic cross-linking under physiological conditions. The cross-linking is catalyzed by horseradish peroxidase (HRP)/glucose oxidase (GOD)-coupled cascade reactions in the presence of glucose and results in 3D dendritic polyglycerol (dPG) scaffolds bound to the surface of glass. These scaffolds continuously release H2O2as long as glucose is present in the system. The resultant polymeric coating is highly stable, bacterial-repellent, and functions under physiological conditions. Challenged with high loads of bacteria (OD540= 1.0), this novel hydrogel and glucose-amended coating reduced the cell viability of Pseudomonas putida (Gram-negative) by 100% and Staphylococcus aureus (Gram-positive) by ≥40%, respectively. Moreover, glucose-stimulated production of H2O2by the coating system was sufficient to kill both test bacteria (at low titers) with >99.99% efficiency within 24 h. In the presence of glucose, this platform produces a coating with high effectiveness against bacterial adhesion and survival that can be envisioned for the applications in the glucose-associated medical/oral devices.
authors.affiliation: Institut für Chemie und Biochemie, Freie Universität Berlin , Takustraße 3, 14195 Berlin, Germany.|Chair of Molecular Biotechnology, Institute of Microbiology, Technische Universität Dresden , Zellescher Weg 20b, 01217 Dresden, Germany.
authors.forename: Changzhu
authors.initials: C
authors.lastname: Wu
authors.affiliation: Federal Institute for Materials Research and Testing (BAM), 12200 Berlin, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Schwibbert
authors.affiliation: Institut für Chemie und Biochemie, Freie Universität Berlin , Takustraße 3, 14195 Berlin, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Achazi
authors.affiliation: Federal Institute for Materials Research and Testing (BAM), 12200 Berlin, Germany.
authors.forename: Petra
authors.initials: P
authors.lastname: Landsberger
authors.affiliation: Federal Institute for Materials Research and Testing (BAM), 12200 Berlin, Germany.
authors.forename: Anna
authors.initials: A
authors.lastname: Gorbushina
authors.affiliation: Institut für Chemie und Biochemie, Freie Universität Berlin , Takustraße 3, 14195 Berlin, Germany.
authors.forename: Rainer
authors.identifier: 0000-0003-3840-162X
authors.initials: R
authors.lastname: Haag
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001422:Bacterial Adhesion; D020099:Coated Materials, Biocompatible / Q000737:chemistry; D005898:Glass / Q000737:chemistry*; D005947:Glucose / Q000378:metabolism; D005949:Glucose Oxidase / Q000378:metabolism*; D005990:Glycerol / Q000737:chemistry*; D006735:Horseradish Peroxidase / Q000378:metabolism*; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D006861:Hydrogen Peroxide / Q000378:metabolism; D010084:Oxidation-Reduction; D011108:Polymers / Q000737:chemistry*; D016958:Pseudomonas putida / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D020099:Coated Materials, Biocompatible; D011108:Polymers; C043941:polyglycerol; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006861:Hydrogen Peroxide; D005949:Glucose Oxidase; D006735:Horseradish Peroxidase; D005947:Glucose; D005990:Glycerol