title: The amino acid transporter, SLC1A3, is plasma membrane-localised in adipocytes and its activity is insensitive to insulin.
abstract: The hormone insulin coordinates the catabolism of nutrients by protein phosphorylation. Phosphoproteomic analysis identified insulin-responsive phosphorylation of the Glu/Asp transporter SLC1A3/EAAT1 in adipocytes. The role of SLC1A3 in adipocytes is not well-understood. We show that SLC1A3 is localised to the plasma membrane and the major regulator of acidic amino acid uptake in adipocytes. However, its localisation and activity were unaffected by insulin or mutation of the insulin-regulated phosphosite. The latter was also observed using a heterologous expression system in Xenopus laevis oocytes. Thus, SLC1A3 maintains a constant import of acidic amino acids independently of nutritional status in adipocytes.
authors.affiliation: School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.
authors.forename: James R
authors.initials: JR
authors.lastname: Krycer
authors.affiliation: School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.
authors.forename: Daniel J
authors.initials: DJ
authors.lastname: Fazakerley
authors.affiliation: Discipline of Pharmacology, Sydney Medical School, The University of Sydney, NSW, Australia.
authors.forename: Rosemary J
authors.initials: RJ
authors.lastname: Cater
authors.affiliation: School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.
authors.forename: Kristen
authors.initials: K
authors.lastname: C Thomas
authors.affiliation: School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.
authors.forename: Sheyda
authors.initials: S
authors.lastname: Naghiloo
authors.affiliation: School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.
authors.forename: James G
authors.initials: JG
authors.lastname: Burchfield
authors.affiliation: School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.
authors.forename: Sean J
authors.initials: SJ
authors.lastname: Humphrey
authors.affiliation: Discipline of Pharmacology, Sydney Medical School, The University of Sydney, NSW, Australia.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Vandenberg
authors.affiliation: Discipline of Pharmacology, Sydney Medical School, The University of Sydney, NSW, Australia.
authors.forename: Renae M
authors.initials: RM
authors.lastname: Ryan
authors.affiliation: School of Life and Environmental Sciences and Charles Perkins Centre, The University of Sydney, NSW, Australia.|Sydney Medical School, The University of Sydney, NSW, Australia.
authors.forename: David E
authors.initials: DE
authors.lastname: James
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects / Q000378:metabolism*; D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism*; D027341:Excitatory Amino Acid Transporter 1 / Q000737:chemistry / Q000378:metabolism*; D057809:HEK293 Cells; D006801:Humans; D007328:Insulin / Q000494:pharmacology*; D051379:Mice; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism; D010766:Phosphorylation / Q000187:drug effects; D014982:Xenopus laevis
chemical_list: D027341:Excitatory Amino Acid Transporter 1; D007328:Insulin
keywords: SLC1A3/EAAT1; adipocyte; insulin