title: The Importance of Standardization on Analyzing Circulating RNA.
abstract: Circulating RNAs, especially microRNAs (miRNAs), have recently emerged as non-invasive disease biomarkers. Despite enthusiasm and numerous reports on disease-associated circulating miRNAs, currently there is no circulating miRNA-based diagnostic in use. In addition, there are many contradictory reports on the concentration changes of specific miRNA in circulation. Here we review the impact of various technical and non-technical factors related to circulating miRNA measurement and elucidate the importance of having a general guideline for sample preparation and concentration measurement in studying circulating RNA.
authors.affiliation: Institute for Systems Biology, 401 Terry Avenue North, Seattle, WA, 98019, USA.
authors.forename: Inyoul
authors.initials: I
authors.lastname: Lee
authors.affiliation: Institute for Systems Biology, 401 Terry Avenue North, Seattle, WA, 98019, USA.
authors.forename: David
authors.initials: D
authors.lastname: Baxter
authors.affiliation: Institute for Systems Biology, 401 Terry Avenue North, Seattle, WA, 98019, USA.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Institute for Systems Biology, 401 Terry Avenue North, Seattle, WA, 98019, USA.
authors.forename: Kelsey
authors.initials: K
authors.lastname: Scherler
authors.affiliation: Institute for Systems Biology, 401 Terry Avenue North, Seattle, WA, 98019, USA. Kwang@systemsbiology.org.
authors.forename: Kai
authors.initials: K
authors.lastname: Wang
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics; D001792:Blood Platelets; D000073888:Cell-Free Nucleic Acids / Q000032:analysis* / Q000302:isolation & purification; D004032:Diet; D015444:Exercise; D005260:Female; D005819:Genetic Markers; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods / Q000592:standards*; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D016133:Polymerase Chain Reaction / Q000379:methods / Q000592:standards; D013048:Specimen Handling / Q000592:standards; D013997:Time Factors
chemical_list: D014408:Biomarkers, Tumor; D000073888:Cell-Free Nucleic Acids; D005819:Genetic Markers; D035683:MicroRNAs