title: Mapping Whole-Transcriptome Splicing in Mouse Hematopoietic Stem Cells.
abstract: Hematopoietic stem cells (HSCs) are rare cells that generate all the various types of blood and immune cells. High-quality transcriptome data have enabled the identification of significant genes for HSCs. However, most genes are expressed in various forms by alternative splicing (AS), extending transcriptome complexity. Here, we delineate AS to determine which isoforms are expressed in mouse HSCs. Our analysis of microarray and RNA-sequencing data includes differential expression of splicing factors that may regulate AS, and a complete map of splicing isoforms. Multiple types of isoforms for known HSC genes and unannotated splicing that may alter gene function are presented. Transcriptome-wide identification of genes and their respective isoforms in mouse HSCs will open another dimension for adult stem cells.
authors.affiliation: The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.
authors.forename: Oron
authors.initials: O
authors.lastname: Goldstein
authors.affiliation: The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.
authors.forename: Karin
authors.initials: K
authors.lastname: Meyer
authors.affiliation: The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.
authors.forename: Yariv
authors.initials: Y
authors.lastname: Greenshpan
authors.affiliation: The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.
authors.forename: Nir
authors.initials: N
authors.lastname: Bujanover
authors.affiliation: The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.
authors.forename: Mili
authors.initials: M
authors.lastname: Feigin
authors.affiliation: Department of Life Sciences, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.
authors.forename: Hadas
authors.initials: H
authors.lastname: Ner-Gaon
authors.affiliation: Department of Life Sciences, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel.
authors.forename: Tal
authors.initials: T
authors.lastname: Shay
authors.affiliation: The Shraga Segal Department for Microbiology, Immunology and Genetics, Faculty of Health Science, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel; The National Institute for Biotechnology in the Negev, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel; Regenerative Medicine and Stem Cell (RMSC) Research Center, Ben-Gurion University of the Negev, Be'er Sheva 84105, Israel. Electronic address: gazitroi@bgu.ac.il.
authors.forename: Roi
authors.initials: R
authors.lastname: Gazit
mesh_terms: D017398:Alternative Splicing; D000818:Animals; D016000:Cluster Analysis; D019295:Computational Biology / Q000379:methods; D004268:DNA-Binding Proteins / Q000235:genetics; D005091:Exons; D020869:Gene Expression Profiling; D063990:Gene Ontology; D006412:Hematopoietic Stem Cells / Q000166:cytology / Q000378:metabolism*; D018398:Homeodomain Proteins / Q000235:genetics; D007438:Introns; D051379:Mice; D010641:Phenotype; D014157:Transcription Factors / Q000235:genetics; D059467:Transcriptome; D064878:Web Browser
chemical_list: D004268:DNA-Binding Proteins; D018398:Homeodomain Proteins; C521133:Prdm16 protein, mouse; D014157:Transcription Factors; C097899:homeobox protein HOXA9
keywords: HSC; ImmGen; RNA-sequencing analysis; adult stem cell splicing; alternative splicing; hematopoietic stem cell; splicing-function; unannotated splicing; whole-transcriptome splicing