title: Effects of weight loss using supplementation with Lactobacillus strains on body fat and medium-chain acylcarnitines in overweight individuals.
abstract: Our previous study showed that supplementation with a combination of Lactobacillus curvatus (L. curvatus) HY7601 and Lactobacillus plantarum (L. plantarum) KY1032 reduced the body weight, body fat percentage, body fat mass and L1 subcutaneous fat area in overweight subjects. We aimed to evaluate whether the changes in adiposity after supplementation with Lactobacillus strains were associated with metabolic intermediates. A randomized, double-blind, placebo-controlled study was conducted on 66 non-diabetic and overweight individuals. Over a 12-week period, the probiotic group consumed 2 g of probiotic powder, whereas the placebo group consumed the same product without the probiotics. To investigate metabolic alterations, we performed plasma metabolomics using ultra-performance liquid chromatography and mass spectrometry (UPLC-LTQ/Orbitrap MS). Probiotic supplementation significantly increased the levels of octenoylcarnitine (C8:1), tetradecenoylcarnitine (C14:1), decanoylcarnitine (C10) and dodecenoylcarnitine (C12:1) compared with the levels from placebo supplementation. In the probiotic group, the changes in the body weight, body fat percentage, body fat mass and L1 subcutaneous fat area were negatively associated with changes in the levels of C8:1, C14:1, C10 and C12:1 acylcarnitines. In overweight individuals, probiotic-induced weight loss and adiposity reduction from the probiotic supplementation were associated with an increase in medium-chain acylcarnitines.
authors.affiliation: Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, Korea.
authors.forename: Minkyung
authors.initials: M
authors.lastname: Kim
authors.affiliation: Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, Korea.
authors.forename: Minjoo
authors.initials: M
authors.lastname: Kim
authors.affiliation: National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, Korea. jhleeb@yonsei.ac.kr and Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, Korea.
authors.forename: Miso
authors.initials: M
authors.lastname: Kang
authors.affiliation: National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, Korea. jhleeb@yonsei.ac.kr and Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Yoo
authors.affiliation: National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, Korea. jhleeb@yonsei.ac.kr and Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, Korea.
authors.forename: Min Sun
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Korea Yakult Co., Ltd, Yongin, Gyeonggi, Korea.
authors.forename: Young-Tae
authors.initials: YT
authors.lastname: Ahn
authors.affiliation: Korea Yakult Co., Ltd, Yongin, Gyeonggi, Korea.
authors.forename: Jae-Hun
authors.initials: JH
authors.lastname: Sim
authors.affiliation: Institute for Health Promotion, Graduate School of Public Health, Yonsei University, Seoul, Korea.
authors.forename: Sun Ha
authors.initials: SH
authors.lastname: Jee
authors.affiliation: Research Center for Silver Science, Institute of Symbiotic Life-TECH, Yonsei University, Seoul, Korea and National Leading Research Laboratory of Clinical Nutrigenetics/Nutrigenomics, Department of Food and Nutrition, College of Human Ecology, Yonsei University, Seoul, Korea. jhleeb@yonsei.ac.kr and Department of Food and Nutrition, Brain Korea 21 PLUS Project, College of Human Ecology, Yonsei University, Seoul, Korea.
authors.forename: Jong Ho
authors.identifier: http://orcid.org/0000-0002-2724-7295
authors.initials: JH
authors.lastname: Lee
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism*; D002331:Carnitine / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000737:chemistry; D019587:Dietary Supplements / Q000032:analysis; D004311:Double-Blind Method; D006801:Humans; D007778:Lactobacillus / Q000502:physiology*; D048191:Lactiplantibacillus plantarum / Q000502:physiology; D050177:Overweight / Q000188:drug therapy* / Q000378:metabolism / Q000503:physiopathology; D019936:Probiotics / Q000008:administration & dosage*; D014280:Triglycerides / Q000378:metabolism; D015431:Weight Loss
chemical_list: D014280:Triglycerides; C116917:acylcarnitine; D002331:Carnitine