title: Klebsiella pneumoniaePlanktonic and Biofilm Reduction by Different Plant Extracts:In VitroStudy.
abstract: This study evaluated the action ofPfaffia paniculataK.,Juglans regiaL., andRosmarius officinalisL. extracts against planktonic form and biofilm ofKlebsiella pneumoniae(ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107UFC/mL ofK. pneumoniaewas distributed into 96-well microplates (n= 10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p< 0.05) of planktonic form and biofilm. P paniculataK.,R. officinalisL., andJ. regiaL. showed reductions in biomass of 55.6, 58.1, and 18.65% and cell viability reduction of 72.4, 65.1, and 31.5%, respectively. The reduction obtained withP. paniculataandR. officinalisextracts was similar to the reduction obtained with chlorhexidine digluconate 2%. In conclusion, all extracts have microbicidal action on the planktonic form but onlyP. paniculataK. andR. officinalisL. were effective against biofilm.
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Lucas
authors.identifier: 0000-0002-2682-2796
authors.initials: L
authors.lastname: Paula-Ramos
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Carlos Eduardo
authors.initials: CE
authors.lastname: da Rocha Santos
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Daphne
authors.initials: D
authors.lastname: Camargo Reis Mello
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Lígia
authors.initials: L
authors.lastname: Nishiama Theodoro
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Felipe Eduardo
authors.initials: FE
authors.lastname: De Oliveira
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Graziella N
authors.initials: GN
authors.lastname: Back Brito
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Juliana Campos
authors.initials: JC
authors.lastname: Junqueira
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Antonio Olavo Cardoso
authors.initials: AOC
authors.lastname: Jorge
authors.affiliation: Department of Biosciences and Oral Diagnosis, São Paulo State University (Unesp), Institute of Science and Technology, São José dos Campos, Francisco José Longo 777, São Dimas, 12245-000 São José dos Campos, SP, Brazil.
authors.forename: Luciane Dias
authors.initials: LD
authors.lastname: de Oliveira
mesh_terms: D027703:Amaranthaceae / Q000737:chemistry; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D018441:Biofilms; D031324:Juglans / Q000737:chemistry; D007711:Klebsiella pneumoniae / Q000187:drug effects* / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000494:pharmacology*; D027542:Rosmarinus / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts