title: The stentable in vitro artery: an instrumented platform for endovascular device development and optimization.
abstract: Although vascular disease is a leading cause of mortality, in vitro tools for controlled, quantitative studies of vascular biological processes in an environment that reflects physiological complexity remain limited. We developed a novel in vitro artery that exhibits a number of unique features distinguishing it from tissue-engineered or organ-on-a-chip constructs, most notably that it allows deployment of endovascular devices including stents, quantitative real-time tracking of cellular responses and detailed measurement of flow velocity and lumenal shear stress using particle image velocimetry. The wall of the stentable in vitro artery consists of an annular collagen hydrogel containing smooth muscle cells (SMCs) and whose lumenal surface is lined with a monolayer of endothelial cells (ECs). The system has in vivo dimensions and physiological flow conditions and allows automated high-resolution live imaging of both SMCs and ECs. To demonstrate proof-of-concept, we imaged and quantified EC wound healing, SMC motility and altered shear stresses on the endothelium after deployment of a coronary stent. The stentable in vitro artery provides a unique platform suited for a broad array of research applications. Wide-scale adoption of this system promises to enhance our understanding of important biological events affecting endovascular device performance and to reduce dependence on animal studies.
authors.affiliation: Hydrodynamics Laboratory (LadHyX), Ecole Polytechnique, Route de Saclay, 91128 Palaiseau, France.
authors.forename: Elizabeth E
authors.identifier: 0000-0001-8116-9585
authors.initials: EE
authors.lastname: Antoine
authors.affiliation: Hydrodynamics Laboratory (LadHyX), Ecole Polytechnique, Route de Saclay, 91128 Palaiseau, France.
authors.forename: François P
authors.initials: FP
authors.lastname: Cornat
authors.affiliation: Hydrodynamics Laboratory (LadHyX), Ecole Polytechnique, Route de Saclay, 91128 Palaiseau, France barakat@ladhyx.polytechnique.fr.
authors.forename: Abdul I
authors.identifier: 0000-0002-7551-3631
authors.initials: AI
authors.lastname: Barakat
mesh_terms: D000818:Animals; D002417:Cattle; D003251:Constriction, Pathologic; D042783:Endothelial Cells / Q000378:metabolism* / Q000473:pathology; D006801:Humans; D008955:Models, Cardiovascular; D009131:Muscle, Smooth, Vascular / Q000378:metabolism* / Q000473:pathology; D032389:Myocytes, Smooth Muscle / Q000378:metabolism* / Q000473:pathology; D014652:Vascular Diseases / Q000378:metabolism* / Q000473:pathology
keywords: collagen I hydrogel; endothelial wound healing; in vitro artery; real-time quantitative cellular imaging; shear stress; stent