title: Micro-RNA signature of lymphovascular space involvement in type 1 endometrial cancer.
abstract: OBJECTIVE
  Lymphovascular space involvement (LVSI) is a major prognostic factor in type 1 endometrial cancer (EC). However, its use has been criticized because of poor subjectivity. MicroRNA signatures have recently been linked to EC pathologic characteristics. The aim of this study was to evaluate whether microRNA profiles of type 1 EC can be related to LVSI status and used as a tool to adapt therapy.
  
  
  METHODS
  MicroRNA expression was assessed by chip analysis and qRT-PCR in 12 formalin-fixed paraffin-embedded grade 2 EC specimens with positive LVSI and in 12 specimens with negative LVSI. Various statistical analyses, including enrichment analysis and a minimum p-value approach, were performed.
  
  
  RESULTS
  The expression levels of microRNAs 34c-5p, -23b-5p, and 23c were significantly lower in the EC with positive LVSI compared to those with negative LVSI. Women with a microRNA-34c-5p fold change <0.15 were more likely to have positive LVSI status (92.3%) compared with those with a microRNA-34c-5p fold change >0.15 (0.0%), p<0.001. Furthermore, women with a microRNA-23b-5p fold change <0.51 were more likely to have positive LVSI status (90.0%) compared with those with a microRNA-23b-5p fold change >0.51 (21.4%), p=0.003.
  
  
  CONCLUSIONS
  This was the first study to investigate the relative expression of microRNA in type 1 EC according to LVSI status. This microRNA expression profile may provide a basis for further study of the microRNA function in EC, and be used as a diagnostic tool for LVSI status.
authors.affiliation: INSERM UMR S 938, University Pierre and Marie Curie, Paris, France. geoffroy.canlorbe@aphp.fr.|Department of Obstetrics and Gynaecology, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), University Pierre and Marie Curie, Paris, France.|Institut Universitaire de Cancérologie (IUC), Paris, France.
authors.forename: Geoffroy
authors.initials: G
authors.lastname: Canlorbe
authors.affiliation: INSERM UMR S 938, University Pierre and Marie Curie, Paris, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Castela
authors.affiliation: Department of Obstetrics and Gynaecology, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), University Pierre and Marie Curie, Paris, France.|INSERM UMR S 707, Epidemiology, Information Systems, Modeling, University Pierre and Marie Curie, Paris, France.
authors.forename: Sofiane
authors.initials: S
authors.lastname: Bendifallah
authors.affiliation: INSERM UMR S 938, University Pierre and Marie Curie, Paris, France.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Faculté de médecine Paris 5 Descartes, Paris, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Lefevre
authors.affiliation: Department of Obstetrics and Gynaecology, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), University Pierre and Marie Curie, Paris, France.|INSERM UMR S 938, University Pierre and Marie Curie, Paris, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Chabbert-Buffet
authors.affiliation: INSERM UMR S 938, University Pierre and Marie Curie, Paris, France.|Department of Dermatology, Cochin University Hospital, Assistance Publique des Hôpitaux de Paris, University René Descartes, Paris, France.
authors.forename: Selim
authors.initials: S
authors.lastname: Aractingi
authors.affiliation: Department of Obstetrics and Gynaecology, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), University Pierre and Marie Curie, Paris, France.|Institut Universitaire de Cancérologie (IUC), Paris, France.|INSERM UMR S 938, University Pierre and Marie Curie, Paris, France.
authors.forename: Emile
authors.initials: E
authors.lastname: DaraÏ
authors.affiliation: INSERM U1016, CNRS 8104, Institut Cochin, University Paris Descartes, Paris, France.
authors.forename: Céline
authors.initials: C
authors.lastname: Méhats
authors.affiliation: Department of Obstetrics and Gynaecology, Tenon University Hospital, Assistance Publique des Hôpitaux de Paris (AP-HP), University Pierre and Marie Curie, Paris, France.|Institut Universitaire de Cancérologie (IUC), Paris, France.|INSERM UMR S 938, University Pierre and Marie Curie, Paris, France.
authors.forename: Marcos
authors.initials: M
authors.lastname: Ballester
mesh_terms: D000368:Aged; D018269:Carcinoma, Endometrioid / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016889:Endometrial Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D005260:Female; D006801:Humans; D008207:Lymphatic Metastasis / Q000473:pathology*; D008208:Lymphatic System / Q000378:metabolism* / Q000473:pathology; D042601:Lymphatic Vessels / Q000378:metabolism / Q000473:pathology; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D009367:Neoplasm Staging
chemical_list: D035683:MicroRNAs