title: Comparison of commercial nanoliquid chromatography columns for fast, targeted mass spectrometry-based proteomics.
abstract: OBJECTIVE
  We compared four commonly used, commercially available reverse phase nanoLC columns for identification/determination of Wnt/β-catenin-related pathway proteins.
  
  
  METHODS
  The columns were: Chromolith®(silica monolith; Merke Millipore, MA, USA), PepMap™ (porous particles; Thermo Fisher Scientific, MA, USA), Accucore™ (solid core particles; Thermo Fisher Scientific) and PepSwift™ (organic monolith; Thermo Fisher Scientific).
  
  
  RESULTS
  The peak capacity of the columns varied from 100 (Pepswift) to 190 (Accucore) (for 30 min gradients). All columns enabled identification/detection of GSK3β and β-catenin in the complex samples. However, even the columns with higher peak capacities could not enable detection of the somewhat less abundant proteins AXIN2 and TNKS2. The monoliths were more prone to retention time instability when sample complexity increased.
  
  
  CONCLUSIONS
  We find that commercial nanoLC columns, although featuring different morphologies and peak capacities, provided surprisingly few practical differences for relatively fast, targeted determination of proteins.
authors.affiliation: Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway; Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway.
authors.forename: Tore
authors.initials: T
authors.lastname: Vehus
authors.affiliation: Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway; Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway.
authors.forename: Kristina Erikstad
authors.initials: KE
authors.lastname: Seterdal
authors.affiliation: Unit for Cell Signaling, SFI-CAST Biomedical Innovation Center, Oslo University Hospital, Rikshospitalet, NO-0027 Oslo, Norway; Unit for Cell Signaling, SFI-CAST Biomedical Innovation Center, Oslo University Hospital, Rikshospitalet, NO-0027 Oslo, Norway.
authors.forename: Stefan
authors.initials: S
authors.lastname: Krauss
authors.affiliation: Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway; Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway.
authors.forename: Elsa
authors.initials: E
authors.lastname: Lundanes
authors.affiliation: Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway; Department of Chemistry, University of Oslo, P.O. 1033 Blindern, NO-0315 Oslo, Norway.
authors.forename: Steven R
authors.initials: SR
authors.lastname: Wilson
keywords: Chromatography; SILAC; mass spectrometry; parallel reaction monitoring; targeted proteomics