title: Adsorptive anodic stripping differential pulse voltammetric determination of CellCept at Fe3O4nanoparticles decorated multi-walled carbon nanotubes modified glassy carbon electrode.
abstract: A simple and sensitive method based on adsorptive anodic stripping differential pulse voltammetry (AASDPV) for the determination of cellcept, using a magnetic Fe3O4nanoparticles and functionalized (carboxylated) multi-walled carbon nanotubes modified glassy carbon electrode (f-MWCNs/Fe3O4/GCE) was developed. In phosphate buffer solution (pH = 5), the voltammogram of cellcept exhibited tow anodic peaks and the well-defined peak at about 0.611 V vs SCE was used for its monitoring. The modified electrode was characterized by different methods such as electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) and cyclic voltammetry (CV). The experimental parameters, such as pH, deposition potential and time, as well as scan rate were optimized. Under the optimized conditions, Ip(μA) was proportional to the cellcept concentration in the range of 0.05-200 μM (R2= 0.9989) with a detection limit of 9.0 nM and limit of quantification of 30.2 nM. The recovery was >98%. The practical analytical utilities of the modified electrode were demonstrated by the determination of cellcept in human urine and blood serum samples. Modified electrode showed an adequate sensitivity and stability for evaluated samples.
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran. Electronic address: mbgholivand2013@gmail.com.
authors.forename: Mohammad Bagher
authors.initials: MB
authors.lastname: Gholivand
authors.affiliation: Department of Analytical Chemistry, Faculty of Chemistry, Razi University, Kermanshah, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Solgi
mesh_terms: D000327:Adsorption; D002244:Carbon / Q000737:chemistry; D058266:Dielectric Spectroscopy; D055664:Electrochemical Techniques; D004566:Electrodes; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D009173:Mycophenolic Acid / Q000032:analysis* / Q000097:blood / Q000652:urine; D037742:Nanotubes, Carbon / Q000737:chemistry*; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D013010:Sonication
chemical_list: D058185:Magnetite Nanoparticles; D037742:Nanotubes, Carbon; D002244:Carbon; D009173:Mycophenolic Acid; D052203:Ferrosoferric Oxide
keywords: Cellcept; Fe(3)O(4) magnetic nanoparticles; MWCNT(S); Stripping voltammetry