title: Visualization of exosome-mediated miR-210 transfer from hypoxic tumor cells.
abstract: Cancer cells actively release exosomes carrying specific cellular components, such as proteins, mRNA, and miRNA, to communicate with various cells in the tumor microenvironment. We visualized exosome-mediated transfer of miR-210 from hypoxic breast cancer cells to neighboring cells using a miR-210 specific reporter system. By in vitro and in vivo visualization, we found that exosomes with miR-210 were transferred to cells in the tumor microenvironment and that miR-210 was involved in expression of vascular remodeling related genes, such as Ephrin A3 and PTP1B, to promote angiogenesis. These results indicate that cellular components, such as miRNAs from hypoxic cancer cells, spread to adjacent cancer cells in the tumor microenvironment via exosomes and influence tumor progression.
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Current affiliation: Department of Radiation Oncology & Medical Physics, Stanford University, CA, USA, 94305.
authors.forename: Kyung Oh
authors.initials: KO
authors.lastname: Jung
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Cancer Imaging Center, Seoul National University Hospital, Seoul, Korea, 110-799.
authors.forename: Hyewon
authors.initials: H
authors.lastname: Youn
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea, 110-799.
authors.forename: Chul-Hee
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea, 110-799.
authors.forename: Keon Wook
authors.initials: KW
authors.lastname: Kang
authors.affiliation: Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul, Korea, 110-799.|Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea, 110-799.
authors.forename: June-Key
authors.initials: JK
authors.lastname: Chung
mesh_terms: D016475:3T3 Cells; D000818:Animals; D001692:Biological Transport; D001943:Breast Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D003676:Deferoxamine / Q000494:pharmacology; D036384:Ephrin-A3 / Q000235:genetics / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism* / Q000473:pathology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D018613:Microscopy, Confocal; D009389:Neovascularization, Pathologic; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1 / Q000235:genetics / Q000378:metabolism; D000067996:RAW 264.7 Cells; D014162:Transfection; D000072258:Tumor Hypoxia; D059016:Tumor Microenvironment; D000097573:Red Fluorescent Protein
chemical_list: D003676:Deferoxamine; D036384:Ephrin-A3; D049452:Green Fluorescent Proteins; D008156:Luciferases; D008164:Luminescent Proteins; D035683:MicroRNAs; D054562:Protein Tyrosine Phosphatase, Non-Receptor Type 1; D000097573:Red Fluorescent Protein; C549325:MIRN210 microRNA, mouse; C516609:Ptpn1 protein, mouse
keywords: breast cancer; exosome; hypoxia; miR-210; tumor microenvironment