title: SNARE proteins play a role in motor axon guidance in vertebrates and invertebrates.
abstract: Axonal growth and guidance rely on correct growth cone responses to guidance cues, both in the central nervous system (CNS) and in the periphery. Unlike the signaling cascades that link axonal growth to cytoskeletal dynamics, little is known about the cross-talk mechanisms between guidance and membrane dynamics and turnover in the axon. Our studies have shown that Netrin-1/deleted in colorectal cancer signaling triggers exocytosis through the SNARE Syntaxin-1 (STX-1) during the formation of commissural pathways. However, limited in vivo evidence is available about the role of SNARE proteins in motor axonal guidance. Here we show that loss-of-function of SNARE complex members results in motor axon guidance defects in fly and chick embryos. Knock-down of Syntaxin-1, VAMP-2, and SNAP-25 leads to abnormalities in the motor axon routes out of the CNS. Our data point to an evolutionarily conserved role of the SNARE complex proteins in motor axon guidance, thereby pinpointing an important function of SNARE proteins in axonal navigation in vivo. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 963-974, 2017.
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Barcelona, Barcelona, 08028, Spain.|Institut de Recerca Biomèdica de Barcelona (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, 08028, Spain.
authors.forename: Pablo José
authors.initials: PJ
authors.lastname: Barrecheguren
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Barcelona, Barcelona, 08028, Spain.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, 28031, Spain.
authors.forename: Oriol
authors.initials: O
authors.lastname: Ros
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Barcelona, Barcelona, 08028, Spain.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, 28031, Spain.
authors.forename: Tiziana
authors.initials: T
authors.lastname: Cotrufo
authors.affiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, 8057, Switzerland.
authors.forename: Beat
authors.initials: B
authors.lastname: Kunz
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Barcelona, Barcelona, 08028, Spain.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, 28031, Spain.|Vall d'Hebron Institute of Research (VHIR), Barcelona, 08035, Spain.|Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, 08010, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Soriano
authors.affiliation: Department of Cell Biology, Faculty of Biology, University of Barcelona, Barcelona, 08028, Spain.|Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ISCIII, Madrid, 28031, Spain.
authors.forename: Fausto
authors.initials: F
authors.lastname: Ulloa
authors.affiliation: Institute of Molecular Life Sciences, University of Zurich, Zurich, 8057, Switzerland.
authors.forename: Esther T
authors.initials: ET
authors.lastname: Stoeckli
authors.affiliation: Institut de Recerca Biomèdica de Barcelona (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, 08028, Spain.|Institut de Biologia Molecular de Barcelona (IBMB-CSIC), Barcelona, 08028, Spain.|Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Barcelona, 08028, Spain.
authors.forename: Sofia J
authors.initials: SJ
authors.lastname: Araújo
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism*; D001369:Axons / Q000378:metabolism*; D002642:Chick Embryo; D029721:Drosophila Proteins / Q000378:metabolism*; D004331:Drosophila melanogaster; D007150:Immunohistochemistry; D009046:Motor Neurons / Q000378:metabolism*; D000071446:Neuronal Outgrowth / Q000502:physiology*; D050600:SNARE Proteins / Q000378:metabolism*; D013045:Species Specificity
chemical_list: D030161:Avian Proteins; D029721:Drosophila Proteins; D050600:SNARE Proteins
keywords: Drosophila; SNARE proteins; Syntaxin-1; axon guidance; motor axon