title: Subretinal Fluid Levels of Signal-Transduction Proteins and Apoptosis Molecules in Macula-Off Retinal Detachment Undergoing Scleral Buckle Surgery.
abstract: To evaluate signal transduction and early apoptosis protein levels in subretinal fluid collected during scleral buckling surgery for macula-off rhegmatogenous retinal detachment (RRD). Our aim was to assess both their relation with RRD features and their influence on the posttreatment outcome.
  
  
  
  Thirty-three eyes of 33 RRD patients scheduled for scleral buckle surgery were enrolled in the study. Undiluted subretinal fluid samples were collected during surgery and analyzed via magnetic bead-based immunoassay. All patients underwent a complete ophthalmologic evaluation at baseline and at each follow-up visit (months 1, 3, and 6). Moreover, both at baseline and at the postsurgery month 6 visit, the patients were tested by means of spectral-domain optical coherence tomography (SD-OCT) in order to evaluate the average ganglion cell-inner plexiform complex thickness, as well as the photoreceptor inner segment/outer segment junction status.
  
  
  
  Patients' clinical features (retinal detachment size, detachment duration, and occurrence of proliferative vitreoretinopathy) were associated with several early apoptotic factors (caspase-8, caspase-9, and B-cell lymphoma 2 [Bcl-2]-associated death promoter [BAD]). Furthermore, both early apoptosis factors (caspase-8, Bcl-2, and p53) and signal-transduction proteins (ERK 1/2) were found to influence the postsurgery month 3 OCT characteristics.
  
  
  
  Signal-transduction proteins and early apoptosis proteins are associated with different clinical features and postsurgery outcomes.
authors.affiliation: Ophthalmology Clinic, Department of Medicine and Science of Ageing, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Paolo
authors.initials: P
authors.lastname: Carpineto
authors.affiliation: Ophthalmology Clinic, Department of Medicine and Science of Ageing, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Agbeanda
authors.initials: A
authors.lastname: Aharrh-Gnama
authors.affiliation: Ophthalmology Clinic, Department of Medicine and Science of Ageing, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Vincenzo
authors.initials: V
authors.lastname: Ciciarelli
authors.affiliation: Ophthalmology Clinic, Department of Medicine and Science of Ageing, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Enrico
authors.initials: E
authors.lastname: Borrelli
authors.affiliation: Ophthalmology Clinic, Department of Medicine and Science of Ageing, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Petti
authors.affiliation: Ophthalmology Clinic, Department of Medicine and Science of Ageing, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Raffaella
authors.initials: R
authors.lastname: Aloia
authors.affiliation: Experimental Pathology, Department of Medicine and Aging Sciences, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Alessia
authors.initials: A
authors.lastname: Lamolinara
authors.affiliation: Laboratory of Biostatistics, Department of Medical, Oral and Biotechnological Sciences, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Marta
authors.initials: M
authors.lastname: Di Nicola
authors.affiliation: Ophthalmology Clinic, Department of Medicine and Science of Ageing, University G. D'Annunzio of Chieti-Pescara, Chieti, Italy.
authors.forename: Leonardo
authors.initials: L
authors.lastname: Mastropasqua
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D017209:Apoptosis; D053181:Caspase 8 / Q000378:metabolism*; D053453:Caspase 9 / Q000378:metabolism*; D005260:Female; D006801:Humans; D008266:Macula Lutea / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D012163:Retinal Detachment / Q000378:metabolism / Q000473:pathology* / Q000601:surgery; D012189:Retrospective Studies; D012591:Scleral Buckling / Q000379:methods*; D015398:Signal Transduction; D058471:Subretinal Fluid / Q000378:metabolism*; D041623:Tomography, Optical Coherence / Q000379:methods; D014792:Visual Acuity
chemical_list: D053181:Caspase 8; D053453:Caspase 9