title: Transcriptome Analysis Highlights Defense and Signaling Pathways Mediated by Ricepi21Gene with Partial Resistance toMagnaporthe oryzae.
abstract: Rice blast disease is one of the most destructive rice diseases worldwide. Thepi21gene confers partial and durable resistance toMagnaporthe oryzae. However, little is known regarding the molecular mechanisms of resistance mediated by the loss-of-function ofPi21. In this study, comparative transcriptome profiling of thePi21-RNAi transgenic rice line and Nipponbare withM. oryzaeinfection at different time points (0, 12, 24, 48, and 72 hpi) were investigated using RNA sequencing. The results generated 43,222 unique genes mapped to the rice genome. In total, 1109 differentially expressed genes (DEGs) were identified between thePi21-RNAi line and Nipponbare withM. oryzaeinfection, with 103, 281, 209, 69, and 678 DEGs at 0, 12, 24, 48, and 72 hpi, respectively. Functional analysis showed that most of the DEGs were involved in metabolism, transport, signaling, and defense. Among the genes assigned to plant-pathogen interaction, we identified 43 receptor kinase genes associated with pathogen-associated molecular pattern recognition and calcium ion influx. The expression levels of brassinolide-insensitive 1, flagellin sensitive 2, and elongation factor Tu receptor, ethylene (ET) biosynthesis and signaling genes, were higher in thePi21-RNAi line than Nipponbare. This suggested that there was a more robust PTI response inPi21-RNAi plants and that ET signaling was important to rice blast resistance. We also identified 53 transcription factor genes, including WRKY, NAC, DOF, and ERF families that show differential expression between the two genotypes. This study highlights possible candidate genes that may serve a function in the partial rice blast resistance mediated by the loss-of-function ofPi21and increase our understanding of the molecular mechanisms involved in partial resistance againstM. oryzae.
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Zhengjie
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: He
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Haihe
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Haiyan
authors.initials: H
authors.lastname: Qu
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Chenyan
authors.initials: C
authors.lastname: Ma
authors.affiliation: State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences Hangzhou, China.
authors.forename: Shaohong
authors.initials: S
authors.lastname: Qu
keywords: RNA-seq; durable resistance; partial resistance; pi21; rice blast disease