title: Frequency ofTNFA,INFG, andIL10Gene Polymorphisms and Their Association with MalariaVivaxand Genomic Ancestry.
abstract: Polymorphisms in cytokine genes can alter the production of these proteins and consequently affect the immune response. The trihybrid heterogeneity of the Brazilian population is characterized as a condition for the use of ancestry informative markers. The objective of this study was to evaluate the frequency of -1031T>C,-308G>A and -238G>A TNFA,+874 A>T IFNGand -819C>T, and -592C>A IL10gene polymorphisms and their association with malariavivaxand genomic ancestry. Samples from 90vivaxmalaria-infected individuals and 51 noninfected individuals from northern Brazil were evaluated. Genotyping was carried out by using ASO-PCR or PCR/RFLP. The genomic ancestry of the individuals was classified using 48 insertion/deletion polymorphism biallelic markers. There were no differences in the proportions of African, European, and Native American ancestry between men and women. No significant association was observed for the allele and genotype frequencies of the 6 SNPs between malaria-infected and noninfected individuals. However, there was a trend toward decreasing the frequency of individuals carrying theTNF-308Aallele with the increasing proportion of European ancestry. No ethnic-specific SNPs were identified, and there was no allelic or genotype association with susceptibility or resistance tovivaxmalaria. Understanding the genomic mechanisms by which ancestry influences this association is critical and requires further study.
authors.affiliation: Department of Dermatologic, Infectious, and Parasitic Diseases, College of Medicine of São José do Rio Preto, São José do Rio Preto, SP, Brazil; University Center of Rio Preto, UNIRP, São José do Rio Preto, SP, Brazil.
authors.forename: Adriana Antônia da Cruz
authors.identifier: 0000-0001-7342-2739
authors.initials: AA
authors.lastname: Furini
authors.affiliation: Laboratory of Tropical Diseases-Prof. Luiz Jacintho da Silva, Department of Genetics, Evolution and Bioagents, University of Campinas, Campinas, SP, Brazil.
authors.forename: Gustavo Capatti
authors.identifier: 0000-0002-6971-5974
authors.initials: GC
authors.lastname: Cassiano
authors.affiliation: Department of Biology, São Paulo State University, São José do Rio Preto, SP, Brazil.
authors.forename: Marcela
authors.identifier: 0000-0002-9602-9806
authors.initials: M
authors.lastname: Petrolini Capobianco
authors.affiliation: Laboratory of Human and Medical Genetics, Federal University of Pará, Belém, PA, Brazil.
authors.forename: Sidney Emanuel Batista
authors.initials: SE
authors.lastname: Dos Santos
authors.affiliation: Department of Biology, São Paulo State University, São José do Rio Preto, SP, Brazil; Laboratory of Basic Research in Malaria, Section of Parasitology, Evandro Chagas Institute, Belém, PA, Brazil.
authors.forename: Ricardo Luiz
authors.identifier: 0000-0002-8955-3204
authors.initials: RL
authors.lastname: Dantas Machado
mesh_terms: D000328:Adult; D000483:Alleles; D001938:Brazil; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000235:genetics; D005838:Genotype; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007371:Interferon-gamma / Q000235:genetics*; D016753:Interleukin-10 / Q000235:genetics*; D016780:Malaria, Vivax / Q000235:genetics*; D008297:Male; D020641:Polymorphism, Single Nucleotide; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics*; D055815:Young Adult
chemical_list: C573415:IFNG protein, human; C508609:IL10 protein, human; C439524:TNF protein, human; D014409:Tumor Necrosis Factor-alpha; D016753:Interleukin-10; D007371:Interferon-gamma