title: Long Noncoding RNA PANDA Positively Regulates Proliferation of Osteosarcoma Cells.
abstract: A long noncoding RNA, p21-associated ncRNA DNA damage-activated (PANDA), associates with nuclear transcription factor Y subunit alpha (NF-YA) and inhibits its binding to promoters of apoptosis-related genes, thereby repressing apoptosis in normal human fibroblasts. Here, we show that PANDA is involved in regulating proliferation in the U2OS human osteosarcoma cell line.
  
  
  
  U2OS cells were transfected with siRNAs against PANDA 72 h later and they were subjected to reverse transcription-polymerase chain reaction (RT-PCR), quantitative RT-PCR and cell-cycle analysis.
  
  
  
  PANDA was highly expressed in U2OS cells, and its expression was induced by DNA damage. Silencing PANDA caused arrest at the G1phase of the cell cycle, leading to inhibition of cell proliferation. Quantitative RT-PCR showed that silencing PANDA increased mRNA levels of the cyclin-dependent kinase inhibitor p18, which caused G1phase arrest.
  
  
  
  These results suggest that PANDA promotes G1-S transition by repressing p18 transcription, and thus promotes U2OS cell proliferation.
authors.affiliation: Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan ykotake@fuk.kindai.ac.jp.|Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan.
authors.forename: Yojiro
authors.initials: Y
authors.lastname: Kotake
authors.affiliation: Department of Biological and Environmental Chemistry, Faculty of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan.
authors.forename: Taiki
authors.initials: T
authors.lastname: Goto
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan.
authors.forename: Madoka
authors.initials: M
authors.lastname: Naemura
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan.
authors.forename: Yasutoshi
authors.initials: Y
authors.lastname: Inoue
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Okamoto
authors.affiliation: Graduate School of Humanity-Oriented Science and Engineering, Kindai University, Iizuka, Japan.
authors.forename: Keiichiro
authors.initials: K
authors.lastname: Tahara
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D050763:Cyclin-Dependent Kinase Inhibitor p18 / Q000235:genetics / Q000378:metabolism; D004249:DNA Damage; D059585:G1 Phase Cell Cycle Checkpoints; D015972:Gene Expression Regulation, Neoplastic; D006367:HeLa Cells; D006801:Humans; D061986:MCF-7 Cells; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D034622:RNA Interference; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction; D013997:Time Factors; D014158:Transcription, Genetic; D014162:Transfection
chemical_list: C493971:CDKN2C protein, human; D050763:Cyclin-Dependent Kinase Inhibitor p18; D062085:RNA, Long Noncoding; C000612163:long non-coding RNA PANDAR, human
keywords: Long non-coding RNA; PANDA; osteosarcoma; p18; transcription