title: Genotoxicity Assessment of Volatile Organic Compounds in Landfill Gas Emission Using Comet Assay in Higher Terrestrial Plant.
abstract: Genotoxicity model is developed to assess the individual subacute toxicity of benzene, toluene, ethylbenzene, and xylene (BTEX) at very low levels as in a landfill gas. Golden Pothos (Epipremnum aureum), a higher plant, was tested under variation of benzene 54-5656 ng/L, toluene 10-4362 ng/L, ethylbenzene 28-4997 ng/L, xylene 53-4845 ng/L, for 96 h. DNA fragmentation in plant leaves were investigated via comet assay. The results show that DNA migration ratio increased with the BTEX concentrations, but at different rates. The 50% effective concentration (EC50) of DNA fragmentation from the dose-response relationships indicated toluene has the highest EC50value and followed by benzene, xylene and ethylbenzene. Alternatively, ethylbenzene has the highest toxicity unit and followed by xylene, benzene and toluene as described by toxicity unit (TU). In conclusion, comet assay of Pothos can be used in differentiating DNA fragmentation against very low levels of BTEX in the atmosphere. Pothos is recommended for genotoxicity assessment of a low BTEX contaminated atmosphere.
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Kasetsart University, 50 Phaholyothin Rd., Chatuchak, Bangkok, 10900, Thailand.
authors.forename: Veerapas
authors.initials: V
authors.lastname: Na Roi-Et
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Kasetsart University, 50 Phaholyothin Rd., Chatuchak, Bangkok, 10900, Thailand. fengwlc@ku.ac.th.
authors.forename: Wilai
authors.initials: W
authors.lastname: Chiemchaisri
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Kasetsart University, 50 Phaholyothin Rd., Chatuchak, Bangkok, 10900, Thailand.
authors.forename: Chart
authors.initials: C
authors.lastname: Chiemchaisri
mesh_terms: D029064:Araceae / Q000187:drug effects* / Q000235:genetics*; D001554:Benzene / Q000633:toxicity; D001555:Benzene Derivatives / Q000633:toxicity; D020552:Comet Assay; D053938:DNA Fragmentation / Q000187:drug effects*; D004305:Dose-Response Relationship, Drug; D014050:Toluene / Q000633:toxicity; D055549:Volatile Organic Compounds / Q000633:toxicity*; D064091:Waste Disposal Facilities; D014992:Xylenes / Q000633:toxicity
chemical_list: D001555:Benzene Derivatives; D055549:Volatile Organic Compounds; D014992:Xylenes; D014050:Toluene; D001554:Benzene; C004912:ethylbenzene
keywords: BTEX; Comet assay; Genotoxicity; Landfill gas; Plant; VOCs