title: Effects of extraction solvents on photoluminescent properties of eysenhardtia polystachia and their potential usage as biomarker.
abstract: Currently, nanomaterials had been used for several applications; one of them is as bio-markers. These nanomaterials contain fluorescent compounds as effective indicators for imaging and other applications in Biotechnology. In previous studies, we proposed a functionalized nanomaterial-based biomarker from silica and Eysenhardtia Polystachia, a medicinal tree known in Mexico as "palo azul" (Kidneywood). Our previous results showed the feasibility of the nanomaterial obtained as bio-marker. In this article, our purpose is to evaluate the effects of extraction solvents on fluorescence of that biomarker. The photoluminescence (PL) effect was evaluated at different pH (4, 7.4 and 8); four extraction solvents, ethanol, methanol, methanol-ethanol and methanol-ethanol-water were evaluated. A molecular dynamics simulation was performed to recognize molecular interaction between the compounds of the extracts with solvent molecules and to investigate the solvent molecules effect on photoluminescence spectra. The results were also compared with rhodamine 6G and we found that, at physiological pH (7.4), the fluorescent-coated silica nanoparticles obtained were also stable. We found that extraction solvents could be used for obtaining different nanomaterials for specific applications, and also found the best extraction solvent for obtaining EP nanomaterials for health care applications, specifically for imaging techniques.
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla No. 3001, Querétaro, Quéretaro CP 76230, Mexico.
authors.forename: M G
authors.initials: MG
authors.lastname: Ferreira-García
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla No. 3001, Querétaro, Quéretaro CP 76230, Mexico.
authors.forename: A R
authors.initials: AR
authors.lastname: Hernandez-Martinez
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla No. 3001, Querétaro, Quéretaro CP 76230, Mexico.
authors.forename: R
authors.initials: R
authors.lastname: Esparza
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla No. 3001, Querétaro, Quéretaro CP 76230, Mexico.
authors.forename: G A
authors.initials: GA
authors.lastname: Molina
authors.affiliation: Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV), Unidad Querétaro, Apdo. Postal 1-798, Querétaro, Querétaro, C.P. 76001, Mexico.
authors.forename: F
authors.initials: F
authors.lastname: Rodriguez-Melgarejo
authors.affiliation: Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV), Unidad Querétaro, Apdo. Postal 1-798, Querétaro, Querétaro, C.P. 76001, Mexico.
authors.forename: S
authors.initials: S
authors.lastname: Jiménez
authors.affiliation: Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, Boulevard Juriquilla No. 3001, Querétaro, Quéretaro CP 76230, Mexico. Electronic address: miries@fata.unam.mx.
authors.forename: M
authors.initials: M
authors.lastname: Estevez
mesh_terms: D015415:Biomarkers / Q000032:analysis*; D000431:Ethanol / Q000737:chemistry; D007887:Fabaceae / Q000737:chemistry* / Q000378:metabolism; D006863:Hydrogen-Ion Concentration; D000432:Methanol / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry*; D011789:Quantum Theory; D012235:Rhodamines / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D013056:Spectrophotometry, Ultraviolet; D013059:Spectrum Analysis, Raman; D055672:Static Electricity; D014867:Water / Q000737:chemistry
chemical_list: D015415:Biomarkers; D010936:Plant Extracts; D012235:Rhodamines; D012997:Solvents; C026188:rhodamine 6G; D014867:Water; D000431:Ethanol; D012822:Silicon Dioxide; D000432:Methanol
keywords: Bio-marker; Extraction solvents; Eysenhardtia Polystachia; Nanomaterials; Photoluminescence; Rhodamine 6G