title: Influence of microRNA‑34a on proliferation, invasion and metastasis of HCT116 cells.
abstract: The aim of the present study was to investigate the role of microRNA (miR)‑34a expression in the proliferation, invasion and metastasis of colon cancer and its underlying mechanisms. HCT116 cells were cultured in high‑sugar Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum and 1000 U/ml penicillin‑streptomycin. Following digestion and resuspension, the cells were used for transfection, expression and western blot analysis. HCT116 cells from miR‑34a transfection, negative control and blank control groups were seeded into a 96‑well plate at a density of 105 cells/ml, and 200 µl complete DMEM was added. The data are presented as the mean ± standard error. A one‑way analysis of variance was performed to compare groups. miR‑34a‑HCT116 cells demonstrated significantly increased expression levels of miR‑34a. The proliferation of HCT116 cells with overexpression of miR‑34a was significantly inhibited to 0.49±0.11 compared with the blank control group (P<0.001). Compared with the blank control and negative control groups, the protein expression levels of B‑cell lymphoma 2 (Bcl‑2) were markedly reduced in the miR‑34a transfected group. Furthermore, the protein expression levels of Bcl‑2‑associated X protein were significantly increased and those of matrix metalloproteinase (MMP)‑2 and MMP‑9 were markedly reduced in the miR‑34a transfected group, MMP‑9 to a greater extent. The present study suggested that overexpression of miR‑34a may inhibit the proliferation, invasion and metastasis of HCT116 cells.
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Shuming
authors.initials: S
authors.lastname: Lu
authors.affiliation: Laboratory Center for Diagnostics, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Yingyan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Shibin
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Zhao
authors.affiliation: Department of Gastroenterology, The First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, P.R. China.
authors.forename: Xiaosheng
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Pathology, Dalian Medical University, Dalian, Liaoning 116044, P.R. China.
authors.forename: Bo
authors.initials: B
authors.lastname: Song
mesh_terms: D049109:Cell Proliferation; D003106:Colon / Q000473:pathology*; D003110:Colonic Neoplasms / Q000235:genetics* / Q000473:pathology*; D015972:Gene Expression Regulation, Neoplastic; D045325:HCT116 Cells; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000235:genetics; D020780:Matrix Metalloproteinase 9 / Q000235:genetics; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness / Q000235:genetics*; D051028:bcl-2-Associated X Protein / Q000235:genetics
chemical_list: C522425:MIRN34 microRNA, human; D035683:MicroRNAs; D051028:bcl-2-Associated X Protein; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9