title: The integration of GC-MS and LC-MS to assay the metabolomics profiling in Panax ginseng and Panax quinquefolius reveals a tissue- and species-specific connectivity of primary metabolites and ginsenosides accumulation.
abstract: The traditional medicine Ginseng mainly including Panax ginseng and Panax quinquefolius is the most widely consumed herbal product in the world. Despite the extensive investigation of biosynthetic pathway of the active compounds ginsenosides, our current understanding of the metabolic interlink between ginsenosides synthesis and primary metabolism at the whole-plant level. In this study, the tissue-specific profiling of primary and the secondary metabolites in two different species of ginseng were investigated by gas chromatography- and liquid chromatography coupled to mass spectrometry. A complex continuous coordination of primary- and secondary-metabolic network was modulated by tissues and species factors during growth. The results showed that altogether 149 primary compounds and 10 ginsenosides were identified from main roots, lateral roots, stems, petioles and leaves in P. ginseng and P. quinquefolius. The partial least squares-discriminate analysis (PLS-DA) revealed obvious compounds distinction among tissue-specific districts relative to species. To survey the dedication of carbon and nitrogen metabolism in different tissues to the accumulation of ginsenosides, we inspected the tissue-specific metabolic changes. Our study testified that the ginsenosides content was dependent on main roots and lateral roots energy metabolism, whereas independent of leaves and petiole photosynthesis during ginsenosides accumulation. When tow species were compared, the results indicated that high rates of C assimilation to C accumulation are closely associated with ginsenosides accumulation in P. ginseng main roots and P. quinquefolius lateral roots, respectively. Taken together, our results suggest that tissue-specific metabolites profiling dynamically changed in process of ginsenosides biosynthesis, which may offer a new train of thoughts to the mechanisms of the ginsenosides biosynthesis at the metabolite level.
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China; Botany Department, Faculty of Science, Menoufia University, Shebin El-koom 32511, Egypt.
authors.forename: Ann
authors.initials: A
authors.lastname: Abozeid
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China.
authors.forename: Yuan-Gang
authors.initials: YG
authors.lastname: Zu
authors.affiliation: Key Laboratory of Plant Ecology, Northeast Forestry University, Harbin 150040, China. Electronic address: tangzh@nefu.edu.cn.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Tang
mesh_terms: D002853:Chromatography, Liquid / Q000379:methods; D008401:Gas Chromatography-Mass Spectrometry / Q000379:methods*; D036145:Ginsenosides / Q000032:analysis / Q000378:metabolism*; D055432:Metabolomics / Q000379:methods*; D005894:Panax / Q000737:chemistry / Q000378:metabolism*; D018515:Plant Leaves / Q000737:chemistry / Q000378:metabolism; D018517:Plant Roots / Q000737:chemistry / Q000378:metabolism; D012503:Saponins / Q000032:analysis / Q000378:metabolism*; D013045:Species Specificity; D014018:Tissue Distribution / Q000502:physiology
chemical_list: D036145:Ginsenosides; C079995:Panax quinquefolium saponin; D012503:Saponins
keywords: Carbon and nitrogen metabolism; Ginsenosides; P. ginseng and P. quinquefolius; Tissue specific