title: MafB is required for development of the hindbrain choroid plexus.
abstract: The choroid plexus (ChP) is a non-neural epithelial tissue that produces cerebrospinal fluid (CSF). The ChP differentiates from the roof plate, a dorsal midline structure of the neural tube. However, molecular mechanisms underlying ChP development are poorly understood compared to neural development. MafB is a bZip transcription factor that is known to be expressed in the roof plate. Here we investigated the role of MafB in embryonic development of the hindbrain ChP (hChP) using Mafb-deficient mice. Immunohistochemical analyses revealed that MafB is expressed in the roof plate and early hChP epithelial cells but its expression disappears at a later embryonic stage. We also found that the Mafb-deficient hChP exhibits delayed differentiation and results in hypoplasia compared to the wild-type hChP. Furthermore, the Mafb-deficient hChP exhibits increased apoptotic cell death and decreased proliferating cells at E12.5, an early stage of hChP development. Collectively, our findings reveal that MafB play an important role in promoting hChP development during embryogenesis.
authors.affiliation: Department of Anatomy and Neuroscience, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan. Electronic address: rkoshida-myz@umin.ac.jp.
authors.forename: Ryusuke
authors.initials: R
authors.lastname: Koshida
authors.affiliation: Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Oishi
authors.affiliation: Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.
authors.forename: Michito
authors.initials: M
authors.lastname: Hamada
authors.affiliation: Department of Anatomy and Neuroscience, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.
authors.forename: Yosuke
authors.initials: Y
authors.lastname: Takei
authors.affiliation: Department of Anatomy and Embryology, Faculty of Medicine, University of Tsukuba, Tsukuba, 305-8575, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Takahashi
mesh_terms: D000818:Animals; D017209:Apoptosis; D002454:Cell Differentiation; D049109:Cell Proliferation; D002831:Choroid Plexus / Q000196:embryology*; D018507:Gene Expression Regulation, Developmental; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D060850:LIM-Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D051258:MafB Transcription Factor / Q000235:genetics* / Q000502:physiology*; D051379:Mice; D008822:Mice, Transgenic; D054259:Neural Tube / Q000378:metabolism; D012249:Rhombencephalon / Q000196:embryology*; D015398:Signal Transduction; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D060850:LIM-Homeodomain Proteins; C489692:Lmx1a protein, mouse; D051258:MafB Transcription Factor; C490406:Mafb protein, mouse; D014157:Transcription Factors
keywords: Hindbrain choroid plexus; Lmx1a; MafB; Roof plate