title: Novel affinity membranes with macrocyclic spacer arms synthesized via click chemistry for lysozyme binding.
abstract: Affinity membrane has great potential for applications in bioseparation and purification. Disclosed herein is the design of a novel affinity membrane with macrocyclic spacer arms for lysozyme binding. The clickable azide-cyclodextrin (CD) arms and clickable alkyne ethylene-vinyl alcohol (EVAL) chains are designed and prepared. By the azide-alkyne click reaction, the EVAL-CD-ligands affinity membranes with CD spacer arms in three-dimensional micro channels have been successfully fabricated. The FT-IR, XPS, NMR, SEM and SEM-EDS results give detailed information of structure evolution. The abundant pores in membrane matrix provide efficient working channels, and the introduced CD arms with ligands (affinity sites) provide supramolecular atmosphere. Compared with that of raw EVAL membrane, the adsorption capacity of EVAL-CD-ligands membrane (26.24mg/g) show a triple increase. The study indicates that three effects (inducing effect, arm effect, site effect) from CD arms render the enhanced performance. The click reaction happened in membrane matrix in bulk. The effective lysozyme binding and higher adsorption performance of affinity membranes described herein compared with other reported membranes are markedly related with the proposed strategy involving macrocyclic spacer arms and supramolecular working channels.
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China. Electronic address: phdlinligang@163.com.
authors.forename: Ligang
authors.initials: L
authors.lastname: Lin
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China.
authors.forename: Hui
authors.initials: H
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China.
authors.forename: Yonghui
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China.
authors.forename: Xihui
authors.initials: X
authors.lastname: Bian
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China; Department of Chemical Engineering, University of Waterloo, Waterloo, ONT., N2L 3G1, Canada.
authors.forename: Qingping
authors.initials: Q
authors.lastname: Xin
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China; Department of Chemical Engineering, University of Waterloo, Waterloo, ONT., N2L 3G1, Canada.
authors.forename: Bowen
authors.initials: B
authors.lastname: Cheng
authors.affiliation: Department of Chemical Engineering, University of Waterloo, Waterloo, ONT., N2L 3G1, Canada.
authors.forename: Xianshe
authors.initials: X
authors.lastname: Feng
authors.affiliation: State Key Laboratory of Separation Membranes and Membrane Processes, Tianjin Polytechnic University, Tianjin 300387, PR China.
authors.forename: Yuzhong
authors.initials: Y
authors.lastname: Zhang
mesh_terms: D001386:Azides / Q000737:chemistry; D057930:Click Chemistry / Q000379:methods*; D003505:Cyclodextrins / Q000737:chemistry; D047028:Macrocyclic Compounds / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D008567:Membranes, Artificial; D009113:Muramidase / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared; D014753:Vinyl Compounds / Q000737:chemistry
chemical_list: D001386:Azides; D003505:Cyclodextrins; D047028:Macrocyclic Compounds; D008567:Membranes, Artificial; D014753:Vinyl Compounds; D009113:Muramidase
keywords: Affinity membrane; Bioseparation; Click chemistry; Cyclodextrin; Macrocyclic spacer arms