title: The Effects of Petroselinum Crispum on Estrogen Receptor-positive Benign and Malignant Mammary Cells (MCF12A/MCF7).
abstract: Phytoestrogens have controversial effects on hormone-dependent tumors. Herein we investigated the effects of parsley root extract (PCE) on DNA synthesis performance, metabolic activity and cytotoxicity in malignant and benign breast cells.
  
  
  
  The PCE was prepared and analyzed by mass spectrometry. MCF7 and MCF12A cells were incubated with various concentrations of PCE and analyzed for DNA synthesis performance, metabolic activity and cytotoxicity by BrdU proliferation, MTT and LDH assays, respectively.
  
  
  
  PCE was found to contain a substantial ratio of lignans. At a concentration range of 0.01 μg/ml-100 μg/ml the LDH assay analysis showed no significant cytotoxicity of PCE in both cell lines. However, at 500 μg/ml PCE's cytotoxicity was well over 70% of total cell population in both cell lines. According to the BrdU proliferation assay analysis, PCE demonstrated significant DNA synthesis inhibition of up to 80% at concentrations of 10, 50, 100 and 500 μg/ml in both cell lines. Based on the MTT assay analysis, only at a concentration of 500 μg/ml, PCE demonstrated a statistically significant inhibition of cellular metabolic activity of 63% in MCF7 and 75% in MCF12A of their respective normal capacity.
  
  
  
  PCE showed antiproliferative effects in MCF7 and MCF12A cells. Further investigation is required to determine whether this effect can be solely attributed to its phytoestrogens.
authors.affiliation: LMU Munich, Department of Obstetrics and Gynecology, Munich, Germany.
authors.forename: Lennard
authors.initials: L
authors.lastname: Schröder
authors.affiliation: LMU Munich, Department of Obstetrics and Gynecology, Munich, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Koch
authors.affiliation: LMU Munich, Department of Obstetrics and Gynecology, Munich, Germany.
authors.forename: Sven
authors.initials: S
authors.lastname: Mahner
authors.affiliation: LMU Munich, Department of Obstetrics and Gynecology, Munich, Germany.
authors.forename: Bernd P
authors.initials: BP
authors.lastname: Kost
authors.affiliation: LMU Munich, Department of Obstetrics and Gynecology, Munich, Germany.
authors.forename: Simone
authors.initials: S
authors.lastname: Hofmann
authors.affiliation: LMU Munich, Department of Obstetrics and Gynecology, Munich, Germany udo.jeschke@med.uni-muenchen.de.
authors.forename: Udo
authors.initials: U
authors.lastname: Jeschke
authors.affiliation: University of Rostock, Department of Obstetrics and Gynecology, Klinikum Sued, Rostock, Germany.
authors.forename: Jens
authors.initials: J
authors.lastname: Haumann
authors.affiliation: University of Rostock, Department of Obstetrics and Gynecology, Klinikum Sued, Rostock, Germany.
authors.forename: Julian
authors.initials: J
authors.lastname: Schmedt
authors.affiliation: University of Rostock, Department of Obstetrics and Gynecology, Klinikum Sued, Rostock, Germany.
authors.forename: Dagmar Ulrike
authors.initials: DU
authors.lastname: Richter
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000302:isolation & purification / Q000494:pharmacology*; D001943:Breast Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D004261:DNA Replication / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004734:Energy Metabolism / Q000187:drug effects; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D061986:MCF-7 Cells; D042361:Mammary Glands, Human / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D013058:Mass Spectrometry; D028528:Petroselinum / Q000737:chemistry; D048789:Phytoestrogens / Q000302:isolation & purification / Q000494:pharmacology*; D008517:Phytotherapy; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018517:Plant Roots; D010946:Plants, Medicinal; D011960:Receptors, Estrogen / Q000187:drug effects* / Q000378:metabolism
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D048789:Phytoestrogens; D010936:Plant Extracts; D011960:Receptors, Estrogen
keywords: MCF12A; MCF7; Petroselinum crispum; breast cancer; estrogen receptor