title: MIPs-graphene nanoplatelets-MWCNTs modified glassy carbon electrode for the determination of cardiac troponin I.
abstract: An electrochemical sensor with high selectivity in addition to sensitivity was developed for the determination of cardiac troponin I (cTnI), based on the modification of cTnI imprinted polymer film on a glassy carbon electrode (GCE). The sensor was fabricated by layer-by-layer assembled graphene nanoplatelets (GS), multiwalled carbon nanotubes (MWCNTs), chitosan (CS), glutaraldehyde (GA) composites, which can increase the electronic transfer rate and the active surface area to capture a larger number of antigenic proteins. MWCNTs/GS based imprinted polymers (MIPs/MWCNTs/GS) were synthesized by means of methacrylic acid (MAA) as the monomer, ethylene glycol dimethacrylate (EGDMA) as the cross linker α,α'-azobisisobutyronitrile (AIBN) as the initiator and cTnI as the template. In comparison with conventional methods, the proposed electrochemical sensor is highly sensitive for cTnI, providing a better linear response range from 0.005 to 60 ng cm-3and a lower limit of detection (LOD) of 0.0008 ng cm-3under optimal experimental conditions. In addition, the electrochemical sensor exhibited good specificity, acceptable reproducibility and stability. Moreover, satisfactory results were obtained in real human serum samples, indicating that the developed method has the potential to find application in clinical detection of cTnI as an alternative approach.
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Ya
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Shen
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Hai-Shui
authors.initials: HS
authors.lastname: Wang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Jia
authors.initials: J
authors.lastname: Tao
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Jian-Zhi
authors.initials: JZ
authors.lastname: Huang
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510641, People's Republic of China. Electronic address: wanglsh@scut.edu.cn.
authors.forename: Li-Shi
authors.initials: LS
authors.lastname: Wang
mesh_terms: D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques; D004566:Electrodes; D006108:Graphite / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D054802:Molecular Imprinting; D009206:Myocardium / Q000378:metabolism; D049329:Nanostructures / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D015203:Reproducibility of Results; D017550:Spectroscopy, Fourier Transform Infrared; D019210:Troponin I / Q000032:analysis*
chemical_list: D037742:Nanotubes, Carbon; D011108:Polymers; D019210:Troponin I; D002244:Carbon; D006108:Graphite
keywords: Cardiac troponin I; Electrochemical sensor; Graphene nanoplatelets; Molecularly imprinted polymers; Multiwalled carbon nanotubes