title: Topical antiedematogenic and anti-inflammatory effect of Scutia buxifolia Reissek gel and stability study.
abstract: Scutia buxifolia Reissek (Rhamnaceae), popularly known in Brazil as "coronilha", is a plant species used in folk medicine for several disorders, including inflammation. However, no studies have been done with this species to confirm its topical anti-inflammatory action. In this study we evaluate the topical antiedematogenic and anti-inflammatory effects of the gel containing crude extract (CE) and the gel containing ethyl acetate (EtOAc) fraction of S. buxifolia on croton oil or UVB radiation-induced ear edema in mice, and perform gel stability study. Antiedematogenic and anti-inflammatory effects were evaluated through ear edema induced by irritant agent croton oil, UVB irradiation-induced skin injury model and neutrophil infiltration. The gel stability study was performed by analyzing organoleptical aspects, pH, viscosity, and quantification of quercetin and rutin by HPLC. The topical treatment with S. buxifolia gel reduced the ear edema and myeloperoxidase activity. Antiedematogenic and anti-inflammatory effects of S. buxifolia were obtained with concentrations of 0.3, 1 and 3%, with maximal inhibition in the concentration of 1% for gel containing CE (inhibitions of 100, 73±0.05 and 97±0.08% for croton oil- or UVB irradiation-induced ear edema and myeloperoxidase activity, respectively) and EtOAc fraction (inhibitions of 79±0.05, 73±0.05 and 89±0.04% for croton oil- or UVB irradiation-induced ear edema and myeloperoxidase activity, respectively). Such effects may be attributed, at least in part, to rutin and quercetin, as well as other compounds found in this species. No important changes were detected in the stability study, in all aspects analyzed in temperature below 25°C. Our results demonstrate that topically applied S. buxifolia gel presented anti-inflammatory effects, provided that it was maintained at a temperature below 25°C.
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Avenida Roraima 1000, Camobi Campus, Santa Maria, RS 97105-900, Brazil. Electronic address: alineboligon@hotmail.com.
authors.forename: Aline Augusti
authors.initials: AA
authors.lastname: Boligon
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS 97105-900, Brazil.
authors.forename: Laís
authors.initials: L
authors.lastname: da Rosa Moreira
authors.affiliation: Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria, Avenida Roraima 1000, Camobi Campus, Santa Maria, RS 97105-900, Brazil.
authors.forename: Mariana
authors.initials: M
authors.lastname: Piana
authors.affiliation: Department of Clinical and Toxicological Analysis, Health Sciences Center, Federal University of Santa Maria, RS, Brazil.
authors.forename: Marli Matiko Anraku
authors.initials: MM
authors.lastname: de Campos
authors.affiliation: Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria, Camobi Campus, Santa Maria, RS 97105-900, Brazil.
authors.forename: Sara Marchesan
authors.initials: SM
authors.lastname: Oliveira
mesh_terms: D000287:Administration, Topical; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000008:administration & dosage / Q000627:therapeutic use*; D003436:Croton Oil / Q000633:toxicity; D004355:Drug Stability; D004487:Edema / Q000188:drug therapy* / Q000209:etiology; D005782:Gels; D008297:Male; D051379:Mice; D027823:Rhamnaceae / Q000737:chemistry*; D014466:Ultraviolet Rays
chemical_list: D000893:Anti-Inflammatory Agents; D005782:Gels; D003436:Croton Oil
keywords: Coronilha; Croton oil; Edema; Inflammation; Scutia buxifolia; UVB irradiation