title: Prevalence of three Oesophagostomum spp. from Tibetan Pigs analyzed by Genetic Markers of nad1, cox3 and ITS1.
abstract: Parasitic nematodes of Oesophagostomum spp., commonly known as 'nodular worms' are one of the most widely distributed and prevalent emerging zoonotic nematodes. However, little is known about the prevalence and gene characteristics of those parasites in Tibetan pigs. Therefore, a study was carried out to investigate the prevalence, isolation and identification of Oesophagostomum spp from Tibetan pigs by genetic markers of nad1,cox3 and ITS1 for the first time. The results revealed that the infection rate of O. dentatum and O. quadrispinulatum by genetic markers of nad1 was 81.13%; 35 (66.04%); the O. dentatum and O. quadrispinulatum by genetic markers cox3 was 66.04%, and O. dentatum and O. stephanostomum by genetic markers ITS1 was found to be 77.36%. Interestingly, the O. stephanostomum specie was identified and isolated from 90.48% stomach and 69.23% colon samples by genetic markers of ITS1. The present study, for the first time has described the presence and genetic characterization of Oesophagostomun spp of O. dentatum, O. quadrispinulatum and especially O. stephanostomum in Tibetan pigs from the high and remote Tibetan plateau. A public concern should be raised in terms of economical losses and severe public health problem.
authors.forename: Kun
authors.initials: K
authors.lastname: Li
authors.forename: Yanfang
authors.initials: Y
authors.lastname: Lan
authors.forename: Houqiang
authors.initials: H
authors.lastname: Luo
authors.forename: Muhammad
authors.initials: M
authors.lastname: Shahzad
authors.forename: Hui
authors.initials: H
authors.lastname: Zhang
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhang
authors.forename: Dongyu
authors.initials: D
authors.lastname: Liu
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.forename: Yanan
authors.initials: Y
authors.lastname: Hao
authors.forename: Suolang
authors.initials: S
authors.lastname: Sizhu
authors.forename: Jiakui
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D021901:DNA, Intergenic / Q000235:genetics; D003576:Electron Transport Complex IV / Q000235:genetics / Q000378:metabolism*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology; D005819:Genetic Markers; D015801:Helminth Proteins / Q000235:genetics / Q000378:metabolism*; D009245:NADH Dehydrogenase / Q000235:genetics / Q000378:metabolism; D009814:Oesophagostomiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*; D009815:Oesophagostomum / Q000145:classification*; D021122:Protein Subunits; D013552:Swine; D013553:Swine Diseases / Q000453:epidemiology / Q000469:parasitology*; D018609:Tibet / Q000453:epidemiology
chemical_list: D021901:DNA, Intergenic; D005819:Genetic Markers; D015801:Helminth Proteins; D021122:Protein Subunits; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV