title: Lycorine induces apoptosis of bladder cancer T24 cells by inhibiting phospho-Akt and activating the intrinsic apoptotic cascade.
abstract: Lycorine, an alkaloid extracted from Amaryllidaceae genera, exhibits antitumor activities against several human solid-tumor and leukemia cells with extensive influence on various cell signaling molecules. However, the effect of lycorine on bladder cancer has not yet been investigated. In this study, we demonstrated that lycorine induced apoptosis in human bladder cancer T24 cells, an effect that is mediated via inhibition of phospho-Akt expression and the consequent activation of caspase-3 and Bax in vitro. In an in vivo experiment, T24 cells were subcutaneously implanted in the right rear flank of nu/nu mice. Lycorine treatment for 14 days significantly inhibited tumor growth compared with that in controls. Collectively, our findings suggest that lycorine suppressed the Akt pathway and activated the intrinsic apoptotic cascade, leading to the apoptosis of bladder cancer cells. We suggest that lycorine can be a viable therapeutic option for bladder cancer patients.
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Chongshan
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Qiongren
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Xiezhao
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Department of Urology, Fujian Provincial Hospital, Fuzhou, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Jin
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan.
authors.forename: Naijin
authors.initials: N
authors.lastname: Xu
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Abai
authors.initials: A
authors.lastname: Xu
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Yawen
authors.initials: Y
authors.lastname: Xu
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Shaobo
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Faculty of Chemical Engineering and Light Industry, Guangdong University of Technology, Guangzhou, China.
authors.forename: Junxia
authors.initials: J
authors.lastname: Zheng
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Urology, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Department of Urology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan; Okayama Medical Innovation Center, Okayama University, Okayama, Japan. Electronic address: huangpeng509@gmail.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Huang
mesh_terms: D047151:Amaryllidaceae Alkaloids / Q000494:pharmacology*; D000818:Animals; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects*; D053148:Caspase 3 / Q000378:metabolism; D002460:Cell Line; D045744:Cell Line, Tumor; D005260:Female; D006801:Humans; D008819:Mice, Nude; D051059:PTEN Phosphohydrolase / Q000378:metabolism; D010617:Phenanthridines / Q000494:pharmacology*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D001743:Urinary Bladder / Q000166:cytology; D001749:Urinary Bladder Neoplasms / Q000188:drug therapy* / Q000378:metabolism / Q000473:pathology; D023041:Xenograft Model Antitumor Assays
chemical_list: D047151:Amaryllidaceae Alkaloids; D000972:Antineoplastic Agents, Phytogenic; D010617:Phenanthridines; D051057:Proto-Oncogene Proteins c-akt; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; D053148:Caspase 3; C015330:lycorine
keywords: Apoptosis; Bladder cancer; Lycorine; Phospho-Akt