title: A novel procedure to assess the non-enzymatic hydrogen-peroxide antioxidant capacity of metabolites with high UV absorption.
abstract: Assays assessing non-enzymatic hydrogen peroxide antioxidant capacities are often hampered by the high UV absorption of the sample itself. This is a typical problem in studies using plant extracts with high polyphenol content. Our assay is based on comparing the 405 nm absorption of the product of potassium iodine and hydrogen peroxide in the presence and absence of a putative hydrogen peroxide reactive antioxidant. This method is free of interference with either hydrogen peroxide or antioxidant self-absorption and it is also suitable for high-throughput plate reader applications.
authors.affiliation: Department of Plant Biology, Institute of Biology, University of Pécs , Ifjúság u. 6, H-7624 Pécs , Hungary.
authors.forename: Kristóf
authors.initials: K
authors.lastname: Csepregi
authors.affiliation: Department of Plant Biology, Institute of Biology, University of Pécs , Ifjúság u. 6, H-7624 Pécs , Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Hideg
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006861:Hydrogen Peroxide / Q000378:metabolism*; D010936:Plant Extracts / Q000378:metabolism*; D059808:Polyphenols / Q000378:metabolism; D011193:Potassium Iodide / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D059808:Polyphenols; D011193:Potassium Iodide; D006861:Hydrogen Peroxide
keywords: Antioxidant assay; UV absorption; hydrogen peroxide; polyphenol