title: Direct Creation of Biopatterns via a Combination of Laser-Based Techniques and Click Chemistry.
abstract: In this paper, we present the immobilization of thiol-modified aptamers on alkyne- or alkene-terminated silicon nitride surfaces by laser-induced click chemistry reactions. The aptamers are printed onto the surface by laser-induced forward transfer (LIFT), which also induces the covalent bonding of the aptamers by thiol-ene or thiol-yne reactions that occur upon UV irradiation of the thiol-modified aptamers with ns laser pulses. This combination of LIFT and laser-induced click chemistry allows the creation of high-resolution patterns without the need for masks. Whereas the click chemistry already takes place during the printing process (single-step process) by the laser pulse used for the printing process, further irradiation of the LIFT-printed aptamers by laser pulses (two-step process) leads to a further increase in the immobilization efficiency.
authors.affiliation: Department of Physics, National Technical University of Athens , Iroon Polytehneiou 9, Zografou, Athens 15780, Greece.
authors.forename: Marianneza
authors.initials: M
authors.lastname: Chatzipetrou
authors.affiliation: Department of Physics, National Technical University of Athens , Iroon Polytehneiou 9, Zografou, Athens 15780, Greece.
authors.forename: Maria
authors.initials: M
authors.lastname: Massaouti
authors.affiliation: Biomedical Research Foundation of the Academy of Athens , 4 Soranou Ephessiou Street, 115 27 Athens, Greece.
authors.forename: George
authors.initials: G
authors.lastname: Tsekenis
authors.affiliation: Surfix B.V. , Dreijenplein 8, 6703 HB Wageningen, The Netherlands.
authors.forename: Anke K
authors.initials: AK
authors.lastname: Trilling
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University , Stippeneng 4, 6708 WE Wageningen, The Netherlands.
authors.forename: Esther
authors.initials: E
authors.lastname: van Andel
authors.affiliation: Surfix B.V. , Dreijenplein 8, 6703 HB Wageningen, The Netherlands.
authors.forename: Luc
authors.initials: L
authors.lastname: Scheres
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University , Stippeneng 4, 6708 WE Wageningen, The Netherlands.
authors.forename: Maarten M J
authors.initials: MM
authors.lastname: Smulders
authors.affiliation: Laboratory of Organic Chemistry, Wageningen University , Stippeneng 4, 6708 WE Wageningen, The Netherlands.|Department of Chemical and Materials Engineering, King Abdulaziz University , Jeddah 23218, Saudi Arabia.
authors.forename: Han
authors.identifier: 0000-0001-5773-8506
authors.initials: H
authors.lastname: Zuilhof
authors.affiliation: Department of Physics, National Technical University of Athens , Iroon Polytehneiou 9, Zografou, Athens 15780, Greece.
authors.forename: Ioanna
authors.identifier: 0000-0001-6341-4495
authors.initials: I
authors.lastname: Zergioti