title: [Effect of Different Hemapheresis Procedures on Components of Hematopoietic Stem Cells Collected from Healthy Donors].
abstract: OBJECTIVE
  To investigate the effects of different hemapheresis procedures on the components of hematopoietic stem cells(HSCs) collected from helathy donors.
  
  
  METHODS
  twelve donors were underwent stem cell collection from January 2015 to August 2016, and the stem cells were randomly colleted by AutoPBSC procedure of COBE spectra and MNC procedure of the Spectra Optia blood cell separator, the mononuclear cells, CD34+cells, granulocytes, lymphocytes and platelets in the collections were compared.
  
  
  RESULTS
  The circulating blood volume, the acquisition time and dosage of anticoagulants were not significantly different between two procedures. The volume and the mononuclear cell count collected by AutoPBSC procedure were lower than those by the MNC procedure, while the CD34+cell count by AutoPBSC procedure was higher than that by the MNC procedure. More lymphocytes and platelets were collected by AutoPBSC procedure as compared with that by the MNC procedure (P<0.05).
  
  
  CONCLUSIONS
  Compared with MNC procedure of the Spectra Optia blood cell separator, the number of collected stem cells, lymphocytes and platelets are higher by using AutoPBSC procedure of the COBE spectra blood cell separator.
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Chun-Hong
authors.initials: CH
authors.lastname: Sun
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Jian-Na
authors.initials: JN
authors.lastname: Yao
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Liu
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Xiao-Juan
authors.initials: XJ
authors.lastname: Wang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Ma
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China.
authors.forename: Mi-Mi
authors.initials: MM
authors.lastname: Cui
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail: zhangmeimeidmail@163.com.
authors.forename: Mei
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Hematology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, Shaanxi Province, China. E-mail: wangxn99@163.com.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Wang
mesh_terms: D018952:Antigens, CD34; D001792:Blood Platelets; D002469:Cell Separation; D006098:Granulocytes; D006412:Hematopoietic Stem Cells; D006801:Humans; D007937:Leukapheresis; D007958:Leukocyte Count; D008214:Lymphocytes; D009504:Neutrophils; D014019:Tissue Donors
chemical_list: D018952:Antigens, CD34