title: Assessing the Penetrating Abilities of Experimental Preparation with Dental Infiltrant Features Using Optical Microscope: Preliminary Study.
abstract: BACKGROUND
  The aim of the infiltration technique is to penetrate demineralized enamel with a low viscosity resin. Icon® (DMG) is the first ever and so far the only dental infiltrant. Bacteriostaticity is one of the properties that should be inherent in dental infiltrants, but Icon lacks this feature.
  
  
  OBJECTIVE
  The aim of the preliminary study was to properly choose a dye which would allow us to assess the penetrating abilities of our own, experimental preparation with features of a dental infiltrant with bacteriostatic properties and to compare using an optical microscope the depth of infiltration of the designed experimental preparation with the infiltrant available on the market.
  
  
  METHODS
  The preparation is supposed to infiltrate decalcified human enamel and be assessed with an optical microscope. Eosin, neutral fuchsine and methylene blue were added to experimental preparation with dental infiltrant features and to Icon® (DMG) in order to assess the depth of penetration of the experimental solution into the decalcified layers of enamel.
  
  
  RESULTS
  The experimental solution mixes well with eosin, neutral fuchsine, and methylene blue.
  
  
  CONCLUSIONS
  During the preliminary study, the authors concluded that the experimental solution mixes well with methylene blue, neutral fuchsine, and eosin. An addition of eosin to a preparation which infiltrates inner, demineralized enamel layers, facilitates the assessment of such a preparation with an optical microscope. A designed experimental solution with the main ingredients, i.e., 2-hydroxyethyl methacrylate (HEMA) and tetraethylene glycol dimethacrylate (TEGDMA) with a ratio of 75% to 25% penetrates the demineralized (decalcified) inner parts of the enamel and polymerizes when exposed to light. In order to assess the infiltration of the experimental solution into the demineralized enamel layers, it is required to improve the measurement techniques that utilize optical microscopy.
authors.affiliation: Department of Conservative Dentistry with Endodontics, Medical University of Silesia in Katowice, Bytom, Poland.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Skucha-Nowak
authors.affiliation: Department of Orthodontics, School of Medicine with Division of Dentistry in Zabrze, Medical University of Silesia in Katowice, Poland.
authors.forename: Agnieszka
authors.initials: A
authors.lastname: Machorowska-Pieniążek
authors.affiliation: Department of Conservative Dentistry with Endodontics, Medical University of Silesia in Katowice, Bytom, Poland.
authors.forename: Marta
authors.initials: M
authors.lastname: Tanasiewicz
mesh_terms: D004396:Coloring Agents / Q000737:chemistry; D003743:Dental Enamel / Q000187:drug effects; D003764:Dental Materials / Q000494:pharmacology*; D006801:Humans; D008853:Microscopy / Q000295:instrumentation*; D012996:Solutions; D014074:Tooth Calcification / Q000187:drug effects; D017001:Tooth Demineralization / Q000473:pathology
chemical_list: D004396:Coloring Agents; D003764:Dental Materials; D012996:Solutions
keywords: biomaterials; dental infiltrant; experimental preparation; minimally invasive dentistry; optical microscope