title: Delphinidin Prevents Muscle Atrophy and Upregulates miR-23a Expression.
abstract: Delphinidin, one of the major anthocyanidins, shows protective effects against a variety of pathologies, including cancer, inflammation, and muscle atrophy. The purpose of this study was to determine the preventive mechanism of delphinidin on disuse muscle atrophy. In vitro and in vivo models were used to validate the effects of delphinidin on the expression of MuRF1, miR-23a, and NFATc3. Delphinidin suppressed the upregulation of MuRF1 (1.77 ± 0.05 vs 1.03 ± 0.17, P < 0.05) expression and inhibited the downregulation of miR-23a (0.56 ± 0.05 vs 0.94 ± 0.06, P < 0.05) and NFATc3 (0.61 ± 0.02 vs 1.02 ± 0.08, P < 0.01) expression in dexamethasone-treated C2C12 cells. In gastrocnemius, muscle weight loss was prevented by oral administration of delphinidin. Moreover, delphinidin suppressed MuRF1 (3.35 ± 0.13 vs 2.26 ± 0.3, P < 0.01) expression and promoted miR-23a (0.58 ± 0.15 vs 2.25 ± 0.29, P < 0.001) and NFATc3 (0.85 ± 0.17 vs 1.54 ± 0.13, P < 0.001) expressions. Delphinidin intake may prevent disuse muscle atrophy by inducing miR-23a expression and suppressing MuRF1 expression.
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Motoki
authors.initials: M
authors.lastname: Murata
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Haruna
authors.initials: H
authors.lastname: Nonaka
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Satomi
authors.initials: S
authors.lastname: Komatsu
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Megumi
authors.initials: M
authors.lastname: Goto
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Morozumi
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Shuhei
authors.initials: S
authors.lastname: Yamada
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: I-Chian
authors.initials: IC
authors.lastname: Lin
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Shuya
authors.initials: S
authors.lastname: Yamashita
authors.affiliation: Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University , Fukuoka 812-8581, Japan.
authors.forename: Hirofumi
authors.identifier: http://orcid.org/0000-0003-4820-2122
authors.initials: H
authors.lastname: Tachibana
mesh_terms: D000818:Animals; D000872:Anthocyanins / Q000008:administration & dosage*; D005938:Glucocorticoids / Q000009:adverse effects; D006801:Humans; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D009124:Muscle Proteins / Q000235:genetics / Q000378:metabolism; D018482:Muscle, Skeletal / Q000187:drug effects* / Q000378:metabolism; D009133:Muscular Atrophy / Q000188:drug therapy* / Q000235:genetics* / Q000378:metabolism; D050778:NFATC Transcription Factors / Q000235:genetics / Q000378:metabolism; D000070584:Tripartite Motif Proteins / Q000235:genetics / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism; D015854:Up-Regulation / Q000187:drug effects
chemical_list: D000872:Anthocyanins; D005938:Glucocorticoids; D035683:MicroRNAs; C534062:Mirn23b microRNA, mouse; D009124:Muscle Proteins; D050778:NFATC Transcription Factors; C494009:Nfatc3 protein, mouse; D000070584:Tripartite Motif Proteins; C499084:Trim63 protein, mouse; D044767:Ubiquitin-Protein Ligases; C017185:delphinidin
keywords: NFATc3; delphinidin; microRNA; muscle atrophy; ubiquitin ligase