title: SS31 ameliorates age-related activation of NF-κB signaling in senile mice model, SAMP8.
abstract: Aging has been attributed to oxidative stress and inflammatory response, in which NF-κB and Nrf2-ARE signaling pathways play significant roles. Senescence accelerated mouse prone 8 (SAMP8) is generally used an animal model for aging studies. Here, we investigated the NF-κB and Nrf2-ARE signaling pathways in SAMP8 brains at different ages and their responses to SS31 peptide treatment. Thirty six SAMP8 mice were separated into aging groups and SS31-treatment groups. The hippocampus from each mouse was dissected for RNA and protein extraction. Cytokines and ROS levels were measured using ELISA and standardised method. Gene expressions of NF-κB, Nrf2 and HO-1 were measured by RT-qPCR. Total protein amount of NF-κB and HO-1, as well as the concentrations of nuclear and cytoplasmic Nrf2 were measured using Western blots. Our data showed that aging could activate both NF-κB and Nrf2-ARE signaling pathways, which could be suppressed and activated by SS31 treatment respectively. Regression analysis revealed that NF-κB gene expression was the most important parameter predicting aging process and SS31 treatment effects in SAMP8. Our findings suggested that SS31 treatment may modulate the inflammatory and oxidative stress status of the aged brains and exert protective effects during brain aging.
authors.affiliation: Department of Neurology, Hebei General Hospital, Shijiazhuang, Hebei, China.|Graduate School, Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Zhi-Hua
authors.initials: ZH
authors.lastname: Hao
authors.affiliation: School of Medical Sciences, Faculty of Medicine, UNSW Australia, Sydney, Australia.
authors.forename: Yue
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Neurology, Hebei General Hospital, Shijiazhuang, Hebei, China.|Graduate School, Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Mei-Rong
authors.initials: MR
authors.lastname: Wang
authors.affiliation: Department of Neurology, Hebei General Hospital, Shijiazhuang, Hebei, China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Huo
authors.affiliation: Graduate School,Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Jia
authors.affiliation: Department of Neurology, Hebei General Hospital, Shijiazhuang, Hebei, China.
authors.forename: Rong-Fang
authors.initials: RF
authors.lastname: Feng
authors.affiliation: Graduate School, Hebei Medical University, Shijiazhuang, Hebei, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Fan
authors.affiliation: Department of Neurology, Hebei General Hospital, Shijiazhuang, Hebei, China.
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Wang
mesh_terms: D000375:Aging / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology* / Q000523:psychology; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D060825:Cognitive Dysfunction / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000235:genetics* / Q000378:metabolism; D009842:Oligopeptides / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects / Q000235:genetics; D017382:Reactive Oxygen Species / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D009842:Oligopeptides; D017382:Reactive Oxygen Species; C506540:arginyl-2,'6'-dimethyltyrosyl-lysyl-phenylalaninamide
keywords: Gerotarget; NF-κB; SAMP8; SS31; aging; inflammation