title: Extracorporeal shock waves induce osteogenic differentiation of human bone-marrow stromal cells.
abstract: The effects of treatment with shock waves (SW) on osteoblastic cells have already been described. Furthermore, the effects of treatment with SW are also determined by the contextual stimulation of other cell lines, in particular of mesenchymal cells. This is the first experimental study of stimulation of a human mesenchymal stem cell line, taken from bone marrow, using SW (electromagnetic device), with two energy levels. The results showed a significant increase in expression of the main osteoblastic differentiation genes: BMP2, alkaline phosphatase, osteocalcin, COL1A1, RUNX2. The monitoring within 96 hours demonstrated a progressive increase of cell adhesion and an intense cell proliferation at 48 h. The differentiation response and proliferation of stem cells after treatment with SW shows that this therapy is an effective method of regenerative medicine.
authors.affiliation: Department of Medical Sciences of Basis, Neurosciences and Organs of Sense, Course of Motor and Sports Sciences, Faculty of Medicine and Surgery, University of Study of Bari “Aldo Moro”, Bari, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Notarnicola
authors.affiliation: Department of Medical Sciences of Basis, Neurosciences and Organs of Sense, Course of Motor and Sports Sciences, Faculty of Medicine and Surgery, University of Study of Bari “Aldo Moro”, Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Vicenti
authors.affiliation: Department of Medical Sciences of Basis, Neurosciences and Organs of Sense, Course of Motor and Sports Sciences, Faculty of Medicine and Surgery, University of Study of Bari “Aldo Moro”, Bari, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Maccagnano
authors.affiliation: Department of Biomedical Sciences and Human Oncology, University of Bari “Aldo Moro”, Bari, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Silvestris
authors.affiliation: Department of Biomedical Sciences and Human Oncology, University of Bari “Aldo Moro”, Bari, Italy.
authors.forename: P
authors.initials: P
authors.lastname: Cafforio
authors.affiliation: Department of Medical Sciences of Basis, Neurosciences and Organs of Sense, Course of Motor and Sports Sciences, Faculty of Medicine and Surgery, University of Study of Bari “Aldo Moro”, Bari, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Moretti
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001853:Bone Marrow; D001854:Bone Marrow Cells / Q000166:cytology*; D002454:Cell Differentiation; D002478:Cells, Cultured; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology; D010012:Osteogenesis; D017154:Stromal Cells
chemical_list: D000469:Alkaline Phosphatase