title: Ameliorating Effects of Bacillus subtilis ANSB060 on Growth Performance, Antioxidant Functions, and Aflatoxin Residues in Ducks Fed Diets Contaminated with Aflatoxins.
abstract: Bacillus subtilisANSB060 isolated from fish gut is very effective in detoxifying aflatoxins in feed and feed ingredients. The purpose of this research was to investigate the effects ofB. subtilisANSB060 on growth performance, body antioxidant functions, and aflatoxin residues in ducks fed moldy maize naturally contaminated with aflatoxins. A total of 1500 18-d-old male Cherry Valley ducks with similar body weight were randomly assigned to five treatments with six replicates of 50 ducks per repeat. The experiment design consisted of five dietary treatments labeled as C0 (basal diet containing 60% normal maize), M0 (basal diet containing 60% moldy maize contaminated with aflatoxins substituted for normal maize), M500, M1000, and M2000 (M0 +500, 1000 or 2000 g/t aflatoxin biodegradation preparation mainly consisted ofB. subtilisANSB060). The results showed that ducks fed 22.44 ± 2.46 μg/kg of AFB₁ (M0) exhibited a decreasing tendency in average daily gain (ADG) and total superoxide dismutase (T-SOD) activity in serum, and T-SOD and glutathione peroxidase (GSH-Px) activities in the liver significantly decreased along with the appearance of AFB₁ and AFM₁ compared with those in Group C0. The supplementation ofB. subtilisANSB060 into aflatoxin-contaminated diets increased the ADG of ducks (p> 0.05), significantly improved antioxidant enzyme activities, and reduced aflatoxin accumulation in duck liver. In conclusion,Bacillus subtilisANSB060 in diets showed an ameliorating effect to duck aflatoxicosis and may be a promising feed additive.
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. zhangliyuan619@163.com.
authors.forename: Liyuan
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. maqiugang@cau.edu.cn.
authors.forename: Qiugang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: Heilongjiang Animal Science Institute, Qiqihar 161005, China. mashanshan33@foxmail.com.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jyzhang@cau.edu.cn.
authors.forename: Jianyun
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Science, Shanxi University, Taiyuan 030006, China. jiaru.bjdk071@163.com.
authors.forename: Ru
authors.initials: R
authors.lastname: Jia
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. jicheng@cau.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China. zhaolihongcau@cau.edu.cn.
authors.forename: Lihong
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000348:Aflatoxins / Q000008:administration & dosage / Q000032:analysis*; D000821:Animal Feed / Q000032:analysis*; D000818:Animals; D001412:Bacillus subtilis; D004032:Diet / Q000662:veterinary*; D004372:Ducks / Q000254:growth & development / Q000382:microbiology; D005506:Food Contamination / Q000032:analysis / Q000517:prevention & control*; D005516:Food Microbiology; D005517:Foodborne Diseases / Q000628:therapy / Q000662:veterinary; D005979:Glutathione Peroxidase / Q000378:metabolism; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D011897:Random Allocation; D017382:Reactive Oxygen Species / Q000378:metabolism; D013482:Superoxide Dismutase / Q000378:metabolism; D003313:Zea mays / Q000737:chemistry / Q000382:microbiology
chemical_list: D000348:Aflatoxins; D017382:Reactive Oxygen Species; D008315:Malondialdehyde; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase
keywords: Bacillus subtilis ANSB060; aflatoxin B1; aflatoxin biodegradation preparation; ameliorating effects; antioxidant function; growth performance; residue