title: Age-related changes in transcriptional abundance and circulating levels of anti-Mullerian hormone and Sertoli cell count in crossbred and Zebu bovine males.
abstract: Age-related changes in peripheral anti-Mullerian hormone (AMH) concentrations and transcriptional abundance of AMH gene in testicular tissue were studied in crossbred (Holstein Friesian × Tharparkar) and Zebu (Tharparkar) males. In both the breeds, basal AMH concentrations were estimated using ELISA method in blood plasma obtained from six males each at 1, 6, 12, 18, and 24 months age. After blood collection at respective ages, all the males were castrated and expression and immunolocalization of AMH was performed in the testicular tissue. The concentration of AMH in blood plasma was found to be highest at 1 month of age in both crossbred and Zebu males, which subsequently decreased with advancing age. Significantly (P < 0.05) lower concentration of AMH was observed in crossbred as compared with Zebu males at 24 months of age. In line with peripheral AMH concentrations, the expression of AMH gene was also higher (P < 0.05) at 1 month of age, which thereafter declined significantly with advancement of age in crossbred males. Furthermore, the expression of AMH gene differed significantly between Zebu and crossbred males at all the age groups studied. Immunolocalization of AMH in testicular tissue also revealed a stronger expression at 1 month age, which gradually decreased till 24 months of age. The true Sertoli cell count was significantly higher in Zebu compared with crossbred males at all age groups studied except at 6 months age. The relationship between Sertoli cell count and circulating AMH concentrations was negative and significant (r = -0.81; P = 0.004). In conclusion, expression of AMH gene in testicular tissue and peripheral blood concentrations of AMH were higher in young compared with adults in both crossbred and Zebu males; however, the transcriptional abundance and circulating levels of AMH were higher in Zebu compared with crossbred males.
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: S K
authors.initials: SK
authors.lastname: Rajak
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India. Electronic address: ogkumaresan@gmail.com.
authors.forename: A
authors.initials: A
authors.lastname: Kumaresan
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: N M
authors.initials: NM
authors.lastname: Attupuram
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: S
authors.initials: S
authors.lastname: Chhillar
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: R K
authors.initials: RK
authors.lastname: Baithalu
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: S
authors.initials: S
authors.lastname: Nayak
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: L
authors.initials: L
authors.lastname: Sreela
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: Raushan K
authors.initials: RK
authors.lastname: Singh
authors.affiliation: Theriogenology Lab, Animal Reproduction, Gynaecology and Obstetrics, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: U K
authors.initials: UK
authors.lastname: Tripathi
authors.affiliation: Artificial Breeding Research Centre, National Dairy Research Institute, Karnal, Haryana, India.
authors.forename: T K
authors.initials: TK
authors.lastname: Mohanty
authors.affiliation: Department of Biophysics, All India Institute of Medical Sciences, New Delhi, India.
authors.forename: Savita
authors.initials: S
authors.lastname: Yadav
mesh_terms: D000375:Aging / Q000097:blood / Q000235:genetics*; D000818:Animals; D054304:Anti-Mullerian Hormone / Q000097:blood* / Q000235:genetics*; D002417:Cattle / Q000235:genetics / Q000502:physiology*; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005786:Gene Expression Regulation; D008297:Male; D012708:Sertoli Cells / Q000166:cytology*; D014158:Transcription, Genetic
chemical_list: D054304:Anti-Mullerian Hormone
keywords: Age; Anti-Mullerian hormone; Crossbred; Sertoli cell; Testis; Zebu male