title: New approaches towards discrimination of fresh/chilled and frozen/thawed chicken breasts by HADH activity determination: Customized slope fitting and chemometrics.
abstract: Fresh/chilled chicken breasts retail at a higher price than their frozen/thawed counterparts. Verification of the fresh/thawed status of chicken meat is determined by measuring β-hydroxyacyl-Coenzyme A-hydrogenase (HADH) activity present in meat intra-cellular liquids spectrophotometrically. However, considerable numbers of reference samples are required for the current arithmetic method, adding to laboratory costs. Therefore, two alternative mathematical approaches which do not require such reference samples were developed and evaluated: curve fitting and multivariate classification. The approaches were developed using 55 fresh/thawed fillet samples. The performance of the methods was examined by an independent validation set which consisted of 16 samples. Finally, the approach was tested in practice in a market study. With the exception of two minor false classifications, both newly proposed methods performed equally well as the classical method. All three methods were able to identify two apparent fraudulent cases in the market study. Therefore, the experiments showed that the costs of HADH measurements can be reduced by adapting alternative mathematics.
authors.affiliation: Wageningen University and Research, RIKILT Wageningen Research, P.O. Box 230, 6700 AE Wageningen, The Netherlands. Electronic address: rita.boerrigter-eenling@wur.nl.
authors.forename: Rita
authors.initials: R
authors.lastname: Boerrigter-Eenling
authors.affiliation: Wageningen University and Research, RIKILT Wageningen Research, P.O. Box 230, 6700 AE Wageningen, The Netherlands.
authors.forename: Martin
authors.initials: M
authors.lastname: Alewijn
authors.affiliation: Wageningen University and Research, RIKILT Wageningen Research, P.O. Box 230, 6700 AE Wageningen, The Netherlands.
authors.forename: Yannick
authors.initials: Y
authors.lastname: Weesepoel
authors.affiliation: Wageningen University and Research, RIKILT Wageningen Research, P.O. Box 230, 6700 AE Wageningen, The Netherlands; Wageningen University and Research, Food Quality and Design Group, Wageningen University, P.O. Box 17, 6700 AA Wageningen, The Netherlands.
authors.forename: Saskia
authors.initials: S
authors.lastname: van Ruth
mesh_terms: D015094:3-Hydroxyacyl CoA Dehydrogenases / Q000378:metabolism*; D000818:Animals; D002645:Chickens; D005511:Food Handling; D005615:Freezing; D008962:Models, Theoretical; D015999:Multivariate Analysis; D011202:Poultry Products / Q000032:analysis*; D015203:Reproducibility of Results
chemical_list: D015094:3-Hydroxyacyl CoA Dehydrogenases
keywords: Adulteration; HADH; Multivariate statistics; Poultry; Slope fitting