title: Trichoderma sp. spores and Kluyveromyces marxianus cells magnetic separation: Immobilization on chitosan-coated magnetic nanoparticles.
abstract: In the present study, the interactions between chitosan-coated magnetic nanoparticles (C-MNP) and Trichoderma sp. spores as well as Kluyveromyces marxianus cells were studied. By Plackett-Burman design, it was demonstrated that factors which directly influenced on yeast cell immobilization and magnetic separation were inoculum and C-MNP quantity, stirring speed, interaction time, and volume of medium, while in the case of fungal spores, the temperature also was disclosed as an influencing factor. Langmuir and Freundlich models were applied for the mathematical analysis of adsorption isotherms at 30°C. For Trichoderma sp. spore adsorption isotherm, the highest correlation coefficient was observed for lineal function of Langmuir model with a maximum adsorption capacity at 5.00E + 09 spores (C-MNP g-1). Adsorption isotherm of K. marxianus cells was better adjusted to Freundlich model with a constant (Kf) estimated as 2.05E + 08 cells (C-MNP g-1). Both systems may have a novel application in fermentation processes assisted with magnetic separation of biomass.
authors.affiliation: a Nanobioscience, Biorefinery and Bioprocess group, Food Research Department, Chemistry School, Autonomous University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés , Saltillo , Coahuila , México.|b Faculty of Engineering in Mechanics and Production Sciences, Polytechnic School of Litoral , Guayaquil-Ecuador.
authors.forename: Sócrates
authors.initials: S
authors.lastname: Palacios-Ponce
authors.affiliation: c Nanobioscience group, CONACYT - Autonomous University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés , Saltillo , Coahuila , México.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Ramos-González
authors.affiliation: a Nanobioscience, Biorefinery and Bioprocess group, Food Research Department, Chemistry School, Autonomous University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés , Saltillo , Coahuila , México.
authors.forename: Héctor A
authors.initials: HA
authors.lastname: Ruiz
authors.affiliation: d Department of Electronic Microscopy and Optical Materials Properties, CINVESTAV of the National Polytechnic Institute (Saltillo Campus), Av. Industria Metalúrgica, Parque Industrial Saltillo-Ramos Arizpe , Ramos Arizpe , Coahuila , México.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Aguilar
authors.affiliation: a Nanobioscience, Biorefinery and Bioprocess group, Food Research Department, Chemistry School, Autonomous University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés , Saltillo , Coahuila , México.
authors.forename: José L
authors.initials: JL
authors.lastname: Martínez-Hernández
authors.affiliation: a Nanobioscience, Biorefinery and Bioprocess group, Food Research Department, Chemistry School, Autonomous University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés , Saltillo , Coahuila , México.
authors.forename: Elda P
authors.initials: EP
authors.lastname: Segura-Ceniceros
authors.affiliation: a Nanobioscience, Biorefinery and Bioprocess group, Food Research Department, Chemistry School, Autonomous University of Coahuila, Blvd. V. Carranza e Ing. José Cárdenas Valdés , Saltillo , Coahuila , México.
authors.forename: Cristóbal N
authors.identifier: http://orcid.org/0000-0001-5867-8672
authors.initials: CN
authors.lastname: Aguilar
authors.affiliation: e Biotechnology Department, ICIDCA - Cuban Research Institute of Derivatives Sugarcane , La Habana , Cuba.
authors.forename: Georgina
authors.initials: G
authors.lastname: Michelena
authors.affiliation: e Biotechnology Department, ICIDCA - Cuban Research Institute of Derivatives Sugarcane , La Habana , Cuba.
authors.forename: Anna
authors.initials: A
authors.lastname: Ilyina
mesh_terms: D000327:Adsorption; D002469:Cell Separation; D048271:Chitosan / Q000737:chemistry*; D007716:Kluyveromyces / Q000166:cytology*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D013172:Spores, Fungal / Q000302:isolation & purification*; D014242:Trichoderma / Q000166:cytology*
chemical_list: D058185:Magnetite Nanoparticles; D048271:Chitosan
keywords: Adsorption isotherm; Plackett–Burman design; chitosan-coated magnetic nanoparticles; fungus spores; magnetic separation; yeast cells