title: Suitability of a cytotoxicity assay for detection of potentially harmful compounds produced by freshwater bloom-forming algae.
abstract: Detecting harmful bioactive compounds produced by bloom-forming pelagic algae is important to assess potential risks to public health. We investigated the application of a cell-based bioassay: the rainbow trout gill-w1 cytotoxicity assay (RCA) that detects changes in cell metabolism. The RCA was used to evaluate the cytotoxic effects of (1) six natural freshwater lake samples from cyanobacteria-rich lakes in central Ontario, Canada; (2) analytical standards of toxins and noxious compounds likely to be produced by the algal communities in these lakes; and (3) complex mixtures of compounds produced by cyanobacterial and chrysophyte cultures. RCA provided a measure of lake water toxicity that could not be reproduced using toxin or noxious compound standards. RCA was not sensitive to toxins and only sensitive to noxious compounds at concentrations higher than reported environmental averages (EC50≥103nM). Cultured algae produced bioactive compounds that had recognizable dose dependent and toxic effects as indicated by RCA. Toxicity of these bioactive compounds depended on taxa (cyanobacteria, not chrysophytes), growth stage (stationary phase more toxic than exponential phase), location (intracellular more toxic than extracellular) and iron status (cells in high-iron treatment more toxic than cells in low-iron treatment). The RCA provides a new avenue of exploration and potential for the detection of natural lake algal toxic and noxious compounds.
authors.affiliation: Department of Biology, Western University, London, Ontario, Canada N6A 5B7. Electronic address: rsorich@uwo.ca.
authors.forename: Ryan J
authors.initials: RJ
authors.lastname: Sorichetti
authors.affiliation: Department of Biology, Western University, London, Ontario, Canada N6A 5B7. Electronic address: jmclau3@uwo.ca.
authors.forename: Jace T
authors.initials: JT
authors.lastname: McLaughlin
authors.affiliation: Department of Biology, Western University, London, Ontario, Canada N6A 5B7. Electronic address: icreed@uwo.ca.
authors.forename: Irena F
authors.initials: IF
authors.lastname: Creed
authors.affiliation: Department of Biology, Western University, London, Ontario, Canada N6A 5B7; Schulich School of Medicine and Dentistry, Western University, London, Ontario, Canada N6A 5B7. Electronic address: cyano@uwo.ca.
authors.forename: Charles G
authors.initials: CG
authors.lastname: Trick
keywords: Cyanobacteria; Freshwater; Harmful algal bloom; Rainbow trout Gill cytotoxicity assay; Taste and odor compounds; Toxins