title: Enhanced Production of Poly-γ-glutamic Acid by Bacillus licheniformis TISTR 1010 with Environmental Controls.
abstract: Bacillus licheniformis TISTR 1010 was used for glutamic acid-independent production of poly-γ-glutamic acid (γ-PGA). A fed-batch production strategy was developed involving feedings of glucose, citric acid, and ammonium chloride at specified stages of the fermentation. With the dissolved oxygen concentration controlled at ≥50% of air saturation and the pH controlled at ~7.4, the fed-batch operation at 37 °C afforded a peak γ-PGA concentration of 39.9 ± 0.3 g L-1with a productivity of 0.926 ± 0.006 g L-1h-1. The observed productivity was nearly threefold greater than previously reported for glutamic acid-independent production using the strain TISTR 1010. The molecular weight of γ-PGA was in the approximate range of 60 to 135 kDa.
authors.affiliation: Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Road, Ladyaow, Chatuchak, Bangkok, 10900, Thailand.|Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand.
authors.forename: Nuttawut
authors.initials: N
authors.lastname: Kongklom
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Zhongping
authors.initials: Z
authors.lastname: Shi
authors.affiliation: School of Engineering, Massey University, Private Bag 11 222, Palmerston North, New Zealand.
authors.forename: Yusuf
authors.identifier: http://orcid.org/0000-0002-0826-7012
authors.initials: Y
authors.lastname: Chisti
authors.affiliation: Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, 50 Ngam Wong Wan Road, Ladyaow, Chatuchak, Bangkok, 10900, Thailand. sarote.s@ku.ac.th.|Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Kasetsart University, Chatuchak, Bangkok, 10900, Thailand. sarote.s@ku.ac.th.
authors.forename: Sarote
authors.initials: S
authors.lastname: Sirisansaneeyakul
mesh_terms: D000069976:Bacillus licheniformis / Q000254:growth & development*; D003470:Culture Media / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D011099:Polyglutamic Acid / Q000096:biosynthesis*
chemical_list: D003470:Culture Media; D011099:Polyglutamic Acid
keywords: Bacillus licheniformis; Fermentation; Poly-γ-glutamic acid (γ-PGA); Polypeptides; Production kinetics