title: Isolation of lactic acid-forming bacteria from biogas plants.
abstract: Direct molecular approaches provide hints that lactic acid bacteria play an important role in the degradation process of organic material to methanogenetic substrates in biogas plants. However, their diversity in biogas fermenter samples has not been analyzed in detail yet. For that reason, five different biogas fermenters, which were fed mainly with maize silage and manure from cattle or pigs, were examined for the occurrence of lactic acid-forming bacteria. A total of 197 lactic acid-forming bacterial strains were isolated, which we assigned to 21 species, belonging to the genera Bacillus, Clostridium, Lactobacillus, Pediococcus, Streptococcus and Pseudoramibacter-related. A qualitative multiplex system and a real-time quantitative PCR could be developed for most isolates, realized by the selection of specific primers. Their role in biogas plants was discussed on the basis of the quantitative results and on physiological data of the isolates.
authors.affiliation: Institute of Microbiology and Wine Research (IMW), Johannes Gutenberg-Universität of Mainz, Johann-Joachim-Becherweg 15, 55099 Mainz, Germany. Electronic address: bohnj@uni-mainz.de.
authors.forename: Jelena
authors.initials: J
authors.lastname: Bohn
authors.affiliation: Institute of Microbiology and Wine Research (IMW), Johannes Gutenberg-Universität of Mainz, Johann-Joachim-Becherweg 15, 55099 Mainz, Germany.
authors.forename: Aytül
authors.initials: A
authors.lastname: Yüksel-Dadak
authors.affiliation: Test and Research Institute Pirmasens (PFI), Marie-Curie-Straße 19, 66953 Pirmasens, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Dröge
authors.affiliation: Institute of Microbiology and Wine Research (IMW), Johannes Gutenberg-Universität of Mainz, Johann-Joachim-Becherweg 15, 55099 Mainz, Germany.
authors.forename: Helmut
authors.initials: H
authors.lastname: König
mesh_terms: D001407:Bacillus / Q000145:classification / Q000302:isolation & purification; D001419:Bacteria / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D044822:Biodiversity; D056804:Biofuels; D003013:Clostridium / Q000145:classification / Q000302:isolation & purification; D005285:Fermentation; D019344:Lactic Acid / Q000096:biosynthesis*; D007778:Lactobacillus / Q000145:classification / Q000302:isolation & purification; D008372:Manure / Q000382:microbiology; D060885:Multiplex Polymerase Chain Reaction; D010376:Pediococcus / Q000145:classification / Q000302:isolation & purification; D010944:Plants / Q000382:microbiology*; D060888:Real-Time Polymerase Chain Reaction; D013291:Streptococcus / Q000145:classification / Q000302:isolation & purification
chemical_list: D056804:Biofuels; D008372:Manure; D019344:Lactic Acid
keywords: Bacillus; Clostridium; Lactic acid bacteria; Multiplex PCR; Primer development; Quantitative real-time PCR