title: Comparative pharmacokinetics between madecassoside and asiaticoside presented in a standardised extract of Centella asiatica, ECa 233 and their respective pure compound given separately in rats.
abstract: 1. ECa 233, the standardised extract of Centella asiatica, contains not less than 80% triterpenoid glycosides, in a madecassoside:asiaticoside ratio of 1.5 (±0.5):1. 2. The pharmacokinetic comparison of madecassoside and asiaticoside was performed in rats following intravenous and oral administration of ECa 233, or an equivalent dose of the individual compounds. Blood, tissues, urine and faeces were collected after dosing to determine drug and metabolite levels using liquid chromatography-tandem mass spectrometry. 3. Our study demonstrated that plasma levels of madecassoside, and to a lesser extent asiaticoside, were higher after administration of ECa 233 than the corresponding values for the pure compounds. There was a bidirectional interconversion between asiaticoside and madecassoside consistent with the increased exposure of madecassoside and asiaticoside in ECa 233. 4. Both madecassoside and asiaticoside appeared to be widely distributed in several organs and metabolized extensively; following intravenous administration of either compound, approximately 80-90% of the dose was recovered as madecassic acid and asiatic acid in the faeces.
authors.affiliation: a Department of Pharmacology and Physiology , Faculty of Pharmaceutical Sciences, Chulalongkorn University , Bangkok , Thailand.
authors.forename: Patcharaporn
authors.initials: P
authors.lastname: Hengjumrut
authors.affiliation: a Department of Pharmacology and Physiology , Faculty of Pharmaceutical Sciences, Chulalongkorn University , Bangkok , Thailand.
authors.forename: Tosapol
authors.initials: T
authors.lastname: Anukunwithaya
authors.affiliation: b Faculty of Pharmaceutical Sciences , Burapha University , Chonburi , Thailand and.
authors.forename: Mayuree H
authors.initials: MH
authors.lastname: Tantisira
authors.affiliation: a Department of Pharmacology and Physiology , Faculty of Pharmaceutical Sciences, Chulalongkorn University , Bangkok , Thailand.
authors.forename: Boonyong
authors.initials: B
authors.lastname: Tantisira
authors.affiliation: a Department of Pharmacology and Physiology , Faculty of Pharmaceutical Sciences, Chulalongkorn University , Bangkok , Thailand.|c Chulalongkorn University Drug and Health Products Innovation Promotion Centre, Faculty of Pharmaceutical Sciences, Chulalongkorn University , Bangkok , Thailand.
authors.forename: Phisit
authors.initials: P
authors.lastname: Khemawoot
mesh_terms: D000818:Animals; D028041:Centella; D010936:Plant Extracts / Q000378:metabolism*; D051381:Rats; D012015:Reference Standards; D014315:Triterpenes / Q000378:metabolism*
chemical_list: C526146:Centella asiatica extract; C000591488:ECa 233 extract; D010936:Plant Extracts; D014315:Triterpenes; C093443:madecassoside; C004446:asiaticoside
keywords: Asiaticoside; Centella asiatica; ECa 233; madecassoside; pharmacokinetics