title: Paraliobacillus sediminis sp. nov., isolated from East China sea sediment.
abstract: A Gram-strain-positive, facultatively anaerobic, motile, endospore-forming, slightly halophilic bacterium, designated strain 126C4T, was isolated from sediment from the East China Sea. The strain was catalase-positive and oxidase-negative. Optimal growth occurred at 28-30 °C, pH 7.0-7.5 and in the presence of 3-5 % (w/v) NaCl. Phylogenetic analyses, based on 16S rRNA gene sequence comparisons, showed that strain 126C4T was a member of the genus Paraliobacillus, with 16S rRNA gene sequence similarities to Paraliobacillus quinghaiensis YIM-C158T and Paraliobacillus ryukyuensis O15-7T of 96.2 % and 95.3 %, repectively. The DNA G+C content was 39.6 mol%. The strain contained MK-7 as the sole respiratory quinone, anteiso-C15 : 0, C16 : 0, iso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids, and its polar lipid pattern comprised diphosphatidylglycerol, phosphatidylethanolamine, three glycolipids and four unknown phospholipids. On the basis of its phylogenetic position, phenotypic traits and chemotaxonomic characteristics, it is suggested that strain 126C4T represents a novel species of the genus Paraliobacillus, for which the name Paraliobacillus sediminis sp. nov. is proposed. The type strain is 126C4T (=KCTC 33762T=MCCC 1H00136T).
authors.affiliation: Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061, PR China.|Key Laboratory of Marine Sedimentology and Environmental Geology, First Institute of Oceanography, SOA, Qingdao 266061, PR China.|Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Wen-Rui
authors.initials: WR
authors.lastname: Cao
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Ling-Yun
authors.initials: LY
authors.lastname: Guo
authors.affiliation: College of Marine Science, Shandong University at Weihai, Weihai 264209, PR China.
authors.forename: Zong-Jun
authors.initials: ZJ
authors.lastname: Du
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.|MACS-Agharkar Research Institute, G.G. Agarkar Road, Pune 411004, Maharashtra, India.
authors.forename: Anindita
authors.initials: A
authors.lastname: Das
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Gaowa
authors.initials: G
authors.lastname: Saren
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Ming-Yu
authors.initials: MY
authors.lastname: Jiang
authors.affiliation: Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Christopher A
authors.initials: CA
authors.lastname: Dunlap
authors.affiliation: Crop Bioprotection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, US Department of Agriculture, Peoria, IL 61604, USA.
authors.forename: Alejandro P
authors.initials: AP
authors.lastname: Rooney
authors.affiliation: Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China.
authors.forename: Xin-Ke
authors.initials: XK
authors.lastname: Yu
authors.affiliation: Key Laboratory of Marine Sedimentology and Environmental Geology, First Institute of Oceanography, SOA, Qingdao 266061, PR China.|Laboratory for Marine Geology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266061, PR China.
authors.forename: Tie-Gang
authors.initials: TG
authors.lastname: Li
mesh_terms: D001406:Bacillaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D019015:Geologic Sediments / Q000382:microbiology*; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C062629:menaquinone 7