title: Molecular Characterization and Phylogenetic Analysis of Pseudomonas aeruginosa Isolates Recovered from Greek Aquatic Habitats Implementing the Double-Locus Sequence Typing Scheme.
abstract: The recently described double-locus sequence typing (DLST) scheme implemented to deeply characterize the genetic profiles of 52 resistant environmental Pseudomonas aeruginosa isolates deriving from aquatic habitats of Greece. DLST scheme was able not only to assign an already known allelic profile to the majority of the isolates but also to recognize two new ones (ms217-190, ms217-191) with high discriminatory power. A third locus (oprD) was also used for the molecular typing, which has been found to be fundamental for the phylogenetic analysis of environmental isolates given the resulted increased discrimination between the isolates. Additionally, the circulation of acquired resistant mechanisms in the aquatic habitats according to their genetic profiles was proved to be more extent. Hereby, we suggest that the combination of the DLST to oprD typing can discriminate phenotypically and genetically related environmental P. aeruginosa isolates providing reliable phylogenetic analysis at a local level.
authors.affiliation: Central Public Health Laboratory, Hellenic Center for Disease Control and Prevention, Athens, Greece.|Department of Medical Laboratories Technological Educational Institute of Athens, Athens, Greece.|Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Olga
authors.initials: O
authors.lastname: Pappa
authors.affiliation: Department of Medical Laboratories Technological Educational Institute of Athens, Athens, Greece.|Department of Clinical Infection, Microbiology and Immunology, Institute of Infection and Global Health, University of Liverpool, Liverpool, UK.
authors.forename: Apostolos
authors.initials: A
authors.lastname: Beloukas
authors.affiliation: Environmental Microbiology Unit, Department of Public Health, School of Medicine, University of Patras, Patras, Greece.
authors.forename: Apostolos
authors.initials: A
authors.lastname: Vantarakis
authors.affiliation: Department of Medical Laboratories Technological Educational Institute of Athens, Athens, Greece.
authors.forename: Athena
authors.initials: A
authors.lastname: Mavridou
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece.
authors.forename: Anastasia-Maria
authors.initials: AM
authors.lastname: Kefala
authors.affiliation: Department of Molecular Biology and Genetics, Democritus University of Thrace, Alexandroupolis, Greece. agalanis@mbg.duth.gr.
authors.forename: Alex
authors.identifier: 0000-0001-9991-3173
authors.initials: A
authors.lastname: Galanis
mesh_terms: D015373:Bacterial Typing Techniques; D017753:Ecosystem; D006115:Greece; D058885:Multilocus Sequence Typing; D010802:Phylogeny; D011550:Pseudomonas aeruginosa / Q000145:classification*; D014871:Water Microbiology
keywords: Aquatic habitats; DLST; P. aeruginosa; Phylogenesis; oprD