title: Improving cellulase productivity of Penicillium oxalicum RE-10 by repeated fed-batch fermentation strategy.
abstract: Medium optimization and repeated fed-batch fermentation were performed to improve the cellulase productivity by P. oxalicum RE-10 in submerged fermentation. First, Plackett-Burman design (PBD) and central composite design (CCD) were used to optimize the medium for cellulase production. PBD demonstrated wheat bran and NaNO3had significant influences on cellulase production. The CCD results showed the maximum filter paper activity (FPA) production of 8.61U/mL could be achieved in Erlenmeyer flasks. The maximal FPA reached 12.69U/mL by submerged batch fermentation in a 7.5-L stirred tank, 1.76-fold higher than that on the original medium. Then, the repeated fed-batch fermentation strategy was performed successfully for increasing the cellulase productivity from 105.75U/L/h in batch fermentation to 158.38U/L/h. The cellulase activity and the glucan conversion of delignined corn cob residue hydrolysis had no significant difference between the enzymes sampled from different cycles of the repeated fed-batch fermentation and that from batch culture.
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Xiaolong
authors.initials: X
authors.lastname: Han
authors.affiliation: School of Life Science, Qufu Normal University, Qufu 273165, China.
authors.forename: Wenxia
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Liu
authors.affiliation: Department of Bioengineering, Qilu University of Technology, Jinan 250353, China.
authors.forename: Zhonghai
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China.
authors.forename: Piao
authors.initials: P
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, China. Electronic address: quyinbo@sdu.edu.cn.
authors.forename: Yinbo
authors.initials: Y
authors.lastname: Qu
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods*; D002480:Cellulase / Q000378:metabolism; D003470:Culture Media / Q000737:chemistry / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D005285:Fermentation; D006868:Hydrolysis; D009566:Nitrates / Q000378:metabolism; D010407:Penicillium / Q000378:metabolism*; D003313:Zea mays / Q000378:metabolism
chemical_list: D003470:Culture Media; D004043:Dietary Fiber; D009566:Nitrates; C031618:sodium nitrate; D002480:Cellulase
keywords: Cellulase; Penicillium oxalicum; Repeated fed-batch fermentation; Response surface methodology