title: PIK3CA mutations as prognostic factor in squamous cell lung carcinoma.
abstract: Mutation in the PIK3CA gene is reported frequent in squamous cell carcinomas of the lung, but its potential prognostic role is still obscure. We have studied the prognostic importance of PIK3CA mutations as well as the relation to other markers in a large number of early stage lung cancers of squamous carcinoma subtype.
  
  
  
  Tumour tissue was obtained from 308 consecutively operated lung cancer patients with squamous cell carcinoma in the period 2003-2013. DNA was isolated according to standard procedures, and mutation analysis was done with either the SnapShot method and/or using PIK3CA specific primers in the Cobas system. PD-L1-expression was analysed with immunohistochemistry After thorough follow-up (median 67.6 months), overall survival and time to relapse was calculated.
  
  
  
  Tumour tissue from 102 females and 206 males were analysed. 167 (54.2%) were in stage I, 96 (31.2%) in stage II and 45 (14.6%) in stage III. PIK3CA mutation was found in 35 (11.4%) patients, most frequently in exon 20. There were no differences in sex, stage or smoking behaviour between mutated and non-mutated cases. Patients with PIK3CA mutations had a significantly longer overall survival (p=0.042) and time to relapse (p=0.030) than non-mutated cases, and the difference in time to relapse was also retained in stage I-cases (p=0.044). PD-L1-expression was less frequent among mutated cases.
  
  
  
  Our results indicate that PIK3CA mutations may confer a survival advantage in early stage squamous cell lung cancers, but further work is needed to confirm this finding.
authors.affiliation: Dept of Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: Marc
authors.initials: M
authors.lastname: McGowan
authors.affiliation: Dept of Pathology, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: Aleksandra Silye
authors.initials: AS
authors.lastname: Hoven
authors.affiliation: Dept of Pathology, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: Marius
authors.initials: M
authors.lastname: Lund-Iversen
authors.affiliation: Dept of Thoracic Surgery, Oslo University Hospital, Oslo, Norway.
authors.forename: Steinar
authors.initials: S
authors.lastname: Solberg
authors.affiliation: Dept of Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway; Dept of Oncology, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway.
authors.forename: Åslaug
authors.initials: Å
authors.lastname: Helland
authors.affiliation: University of Colorado Cancer Center, Denver, CO, USA.
authors.forename: Fred R
authors.initials: FR
authors.lastname: Hirsch
authors.affiliation: Dept of Cancer Research, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway; Dept of Oncology, Oslo University Hospital-The Norwegian Radium Hospital, Oslo, Norway. Electronic address: ot.brustugun@gmail.com.
authors.forename: Odd Terje
authors.initials: OT
authors.lastname: Brustugun
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D060890:B7-H1 Antigen / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D058534:Class I Phosphatidylinositol 3-Kinases / Q000235:genetics*; D015331:Cohort Studies; D004252:DNA Mutational Analysis; D005091:Exons / Q000235:genetics; D005260:Female; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000473:pathology / Q000601:surgery; D008297:Male; D008875:Middle Aged; D009154:Mutation; D009367:Neoplasm Staging; D011379:Prognosis; D012008:Recurrence; D012189:Retrospective Studies; D015996:Survival Rate
chemical_list: D060890:B7-H1 Antigen; C423236:CD274 protein, human; D058534:Class I Phosphatidylinositol 3-Kinases; C484760:PIK3CA protein, human
keywords: Lung cancer; Mutation; PIK3CA; Prognosis; Squamous cell carcinoma