title: Serum concentrations and gene expression of sirtuin 1 in healthy and slightly overweight subjects after caloric restriction or resveratrol supplementation: A randomized trial.
abstract: BACKGROUND
  Sirtuin 1 (Sirt1) plays an important role in vascular biology, and influences aspects of age-dependent atherosclerosis. In animals, the sirtuin system is strongly influenced by resveratrol and caloric restriction, but its expression in humans is controversial. This study investigated the effects of resveratrol and caloric restriction on Sirt1 serum concentrations and vascular biomarkers in a healthy human population.
  
  
  RESULTS
  Forty-eight healthy participants (24 women) aged 55-65years were randomized to either 30days of resveratrol administration (500mg/day) or caloric restriction (1000cal/day). Blood was collected at baseline and day 30. Laboratory data analyzed were triglycerides, total cholesterol, HDL, VLDL, LDL, apolipoprotein A1, apolipoprotein B, lipoprotein (a), non-esterified fatty acids (NEFA), glucose, insulin, oxidative stress, C-reactive protein, and Sirt1. Expression of the Sirt1 gene was analyzed using real-time PCR. Caloric restriction diminished the abdominal circumference and improved the lipid profile, but not resveratrol intervention. Resveratrol and caloric restriction increased serum concentrations of Sirt1, from 1.06±0.71 to 5.75±2.98ng/mL; p<0.0001, and from 1.65±1.81 to 5.80±2.23ng/mL; p<0.0001, respectively. Sirt1 increased in women and men in both interventions. On the other hand expression of Sirt1 mRNA was not different after caloric restriction and resveratrol treatment.
  
  
  CONCLUSIONS
  Caloric restriction and resveratrol significantly increased plasma concentrations of Sirt1. The long-term impact of these interventions on atherosclerosis should be assessed.
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil. Electronic address: apmansur@usp.br.
authors.forename: Antonio P
authors.initials: AP
authors.lastname: Mansur
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Roggerio
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Marisa F S
authors.initials: MFS
authors.lastname: Goes
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Solange D
authors.initials: SD
authors.lastname: Avakian
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Dalila P
authors.initials: DP
authors.lastname: Leal
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Raul C
authors.initials: RC
authors.lastname: Maranhão
authors.affiliation: Heart Institute (InCor), University of São Paulo Medical School, São Paulo, Brazil.
authors.forename: Célia M C
authors.initials: CMC
authors.lastname: Strunz
mesh_terms: D015415:Biomarkers / Q000097:blood; D031204:Caloric Restriction; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects; D006801:Humans; D008055:Lipids / Q000097:blood; D008297:Male; D008875:Middle Aged; D050177:Overweight / Q000178:diet therapy* / Q000235:genetics / Q000378:metabolism; D011446:Prospective Studies; D000077185:Resveratrol; D056564:Sirtuin 1 / Q000096:biosynthesis / Q000097:blood* / Q000235:genetics*; D013267:Stilbenes / Q000008:administration & dosage*
chemical_list: D015415:Biomarkers; D008055:Lipids; D013267:Stilbenes; C447939:SIRT1 protein, human; D056564:Sirtuin 1; D000077185:Resveratrol
keywords: Caloric restriction; Gene expression; Human population; Resveratrol; Sirtuin 1