title: Collagen V expression is crucial in regional development of the supraspinatus tendon.
abstract: Manipulations in cell culture and mouse models have demonstrated that reduction of collagen V results in altered fibril structure and matrix assembly. A tissue-dependent role for collagen V in determining mechanical function was recently established, but its role in determining regional properties has not been addressed. The objective of this study was to define the role(s) of collagen V expression in establishing the site-specific properties of the supraspinatus tendon. The insertion and midsubstance of tendons from wild type, heterozygous and tendon/ligament-specific null mice were assessed for crimp morphology, fibril morphology, cell morphology, as well as total collagen and pyridinoline cross-link (PYD) content. Fibril morphology was altered at the midsubstance of both groups with larger, but fewer, fibrils and no change in cell morphology or collagen compared to the wild type controls. In contrast, a significant disruption of fibril assembly was observed at the insertion site of the null group with the presence of structurally aberrant fibrils. Alterations were also present in cell density and PYD content. Altogether, these results demonstrate that collagen V plays a crucial role in determining region-specific differences in mouse supraspinatus tendon structure. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:2154-2161, 2016.
authors.affiliation: McKay Orthopaedic Research Laboratory, University of Pennsylvania, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, Pennsylvania, 19104-6081.
authors.forename: Brianne K
authors.initials: BK
authors.lastname: Connizzo
authors.affiliation: Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, Florida, 33612.
authors.forename: Sheila M
authors.initials: SM
authors.lastname: Adams
authors.affiliation: Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, Florida, 33612.
authors.forename: Thomas H
authors.initials: TH
authors.lastname: Adams
authors.affiliation: Department of Molecular Pharmacology and Physiology, Morsani College of Medicine, University of South Florida, Tampa, Florida, 33612.
authors.forename: David E
authors.initials: DE
authors.lastname: Birk
authors.affiliation: McKay Orthopaedic Research Laboratory, University of Pennsylvania, 424 Stemmler Hall, 36th and Hamilton Walk, Philadelphia, Pennsylvania, 19104-6081.
authors.forename: Louis J
authors.initials: LJ
authors.lastname: Soslowsky
mesh_terms: D000818:Animals; D024062:Collagen Type V / Q000235:genetics / Q000378:metabolism*; D008810:Mice, Inbred C57BL; D017006:Rotator Cuff / Q000254:growth & development / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D024062:Collagen Type V
keywords: EDS; collagen; fibril; supraspinatus; tendon