title: Influence of silver content on rifampicin adsorptivity for magnetite/Ag/rifampicin nanoparticles.
abstract: Magnetite nanoparticles (NPs) decorated with silver (magnetite/Ag) are intensively investigated due to their application in the biomedical field. We demonstrate that the increase of silver content on the surface of nanoparticles improves the adsorptivity of antibiotic rifampicin as well as antibacterial properties. The use of ginger extract allowed to improve the silver nucleation on the magnetite surface that resulted in an increase of silver content. Physicochemical and functional characterization of magnetite/Ag NPs was performed. Our results show that 5%-10% of silver content in magnetite/Ag NPs is already sufficient for antimicrobial properties against Streptococcus salivarius and Staphylococcus aureus. The rifampicin molecules on the magnetite/Ag NPs surface made the spectrum of antimicrobial activity wider. Cytotoxicity evaluation of the magnetite/Ag/rifampicin NPs showed no harmful action towards normal human fibroblasts, whereas the effect on human embryonic kidney cell viability was time and dose dependent.
authors.affiliation: NanoBioMedical Centre, Adam Mickiewicz University, Poznań, Poland.
authors.forename: Olena
authors.initials: O
authors.lastname: Ivashchenko
authors.forename: Emerson
authors.initials: E
authors.lastname: Coy
authors.forename: Barbara
authors.initials: B
authors.lastname: Peplinska
authors.forename: Marcin
authors.initials: M
authors.lastname: Jarek
authors.forename: Mikołaj
authors.initials: M
authors.lastname: Lewandowski
authors.forename: Karol
authors.initials: K
authors.lastname: Załęski
authors.forename: Alicja
authors.initials: A
authors.lastname: Warowicka
authors.forename: Anna
authors.initials: A
authors.lastname: Wozniak
authors.forename: Tatiana
authors.initials: T
authors.lastname: Babutina
authors.forename: Justyna
authors.initials: J
authors.lastname: Jurga-Stopa
authors.forename: Janez
authors.initials: J
authors.lastname: Dolinsek
authors.forename: Stefan
authors.initials: S
authors.lastname: Jurga
mesh_terms: D000327:Adsorption / Q000187:drug effects; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020939:Zingiber officinale / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D058185:Magnetite Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D012293:Rifampin / Q000737:chemistry / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects / Q000254:growth & development; D000070001:Streptococcus salivarius / Q000187:drug effects / Q000254:growth & development
chemical_list: D000900:Anti-Bacterial Agents; D058185:Magnetite Nanoparticles; D010936:Plant Extracts; D012834:Silver; D012293:Rifampin