title: The protective effect of resveratrol in the transmission of neuropathic pain mediated by the P2X7receptor in the dorsal root ganglia.
abstract: The P2X7receptor mediates afferent nerve activation and is related to chronic neuropathic pain. Resveratrol (RES) has also been reported to exhibit anti-inflammatory effects. In this study, we investigated the neuroprotective effect of RES on the transmission of neuropathic pain mediated by the P2X7receptor. The P2X7mRNA and protein expression levels in L4-L5 dorsal root ganglia (DRG)s of the chronic constriction injury (CCI) group were significantly higher than those observed in the Ctrl + NS, Sham + RES and Sham groups. RES increased the threshold of thermal and mechanical hypersensitivity in rats with chronic neuropathic pain. The P2X7mRNA and protein expression levels in the CCI + RES group were decreased compared with those in the CCI group. Our results showed that RES inhibited the upregulated co-expression of P2X7and glial fibrillary acidic protein (GFAP, a marker of satellite glial cells) in satellite glial cells of DRG in the CCI group. The results demonstrated that the expression of GFAP was increased in the CCI group and that RES inhibited the upregulated expression of GFAP in the rats in the CCI group. In addition, the phosphorylation levels of p38 and extracellular regulated protein kinases (ERK)1/2 in the CCI group were markedly higher than those observed in the Ctrl + NS, Sham + RES and Sham groups, whereas the phosphorylation levels of p38 and ERK1/2 in CCI + RES group were markedly lower than those observed in the CCI group. RES inhibited BzATP-activated currents in DRG non-neurons in the CCI rats. Our data provide evidence that RES may suppress the transmission of neuropathic pain mediated by the P2X7receptor in the satellite glial cells of dorsal root ganglia.
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China; Key Laboratory of Molecular Medicine of JiangXi, The Second Affiliated Hospital of Nanchang University, Nanchang, PR China.
authors.forename: Jinyan
authors.initials: J
authors.lastname: Xie
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Shenqiang
authors.initials: S
authors.lastname: Rao
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Jia
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China.
authors.forename: Shanhong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Molecular Pharmacology of RWTH Aachen University, Aachen, D-52072, Germany.
authors.forename: Günther
authors.initials: G
authors.lastname: Schmalzing
authors.affiliation: Molecular Pharmacology of RWTH Aachen University, Aachen, D-52072, Germany.
authors.forename: Ralf
authors.initials: R
authors.lastname: Hausmann
authors.affiliation: Pharmacology of Traditional Chinese Medicine of Jinan University, Guangzhou, PR China.
authors.forename: Hong
authors.initials: H
authors.lastname: Nie
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China; Department of Clinical Translational Research, Singapore General Hospital, Singapore.
authors.forename: Guodong
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Basic Medical College of Nanchang University, Nanchang, PR China. Electronic address: liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005727:Ganglia, Spinal / Q000187:drug effects*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D006930:Hyperalgesia / Q000188:drug therapy; D008297:Male; D009437:Neuralgia / Q000188:drug therapy* / Q000378:metabolism; D010147:Pain Measurement / Q000379:methods; D017207:Rats, Sprague-Dawley; D058486:Receptors, Purinergic P2X7 / Q000378:metabolism*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D058486:Receptors, Purinergic P2X7; D013267:Stilbenes; D000077185:Resveratrol
keywords: Dorsal root ganglia; Neuropathic pain; P2X(7) receptor; Resveratrol; Satellite glial cell