title: The expression of DAMP proteins HSP70 and cancer-testis antigen SPAG9 in peripheral blood of patients with HCC and lung cancer.
abstract: There are different views of how the immune system participates in the reaction to cancer. Here, we evaluated expression of DAMP proteins HSP70 and cancer-testis antigen SPAG9 in patients with hepatocellular carcinoma (HCC) and lung cancer to explore tumor immunity. Our analysis showed that levels of HSP70 and SPAG9 antibody were significantly higher in the serum of lung cancer and HCC patients than in the serum of healthy subjects (P < 0.001), but there were no differences in levels of HSP70 antibody in patients and controls. Levels of serum SPAG9 antibody in newly diagnosed lung cancer patients were significantly higher than in treated lung cancer patients (P < 0.05), but there were no differences in levels of HSP70 or HSP70 antibody. Levels of serum HSP70 and SPAG9 antibody, but not HSP70 antibody, were also higher in hepatitis/cirrhosis patients than in healthy subjects (P = 0.005, P < 0.001). Levels of serum SPAG9 antibody were significantly higher in HCC patients than in hepatitis/cirrhosis patients, but there were no differences in HSP70 or HSP70 antibody levels. Finally, levels of serum HSP70 and SPAG9 antibody were significantly higher in HCC patients than in lung cancer patients (P < 0.05, P < 0.001). These results indicate that cancer-testis antigen SPAG9 induces a strong humoral immune response in cancer patients but HSP70 does not. These results show that SPAG9 has potential as a tumor-specific biomarker.
authors.affiliation: Clinical Laboratory, Hunan Provincial Second People's Hospital, 427 Furong Road of Changsha, Changsha, Hunan, 410007, China. 13808481211@163.com.|Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410007, China. 13808481211@163.com.
authors.forename: Biqiong
authors.initials: B
authors.lastname: Ren
authors.affiliation: Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410007, China.
authors.forename: Shudi
authors.initials: S
authors.lastname: Luo
authors.affiliation: Clinical Laboratory, Hunan Provincial Second People's Hospital, 427 Furong Road of Changsha, Changsha, Hunan, 410007, China.|Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410007, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Xu
authors.affiliation: Clinical Laboratory, Hunan Provincial Second People's Hospital, 427 Furong Road of Changsha, Changsha, Hunan, 410007, China.|Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410007, China.
authors.forename: Guoying
authors.initials: G
authors.lastname: Zou
authors.affiliation: Clinical Laboratory, Hunan Provincial Second People's Hospital, 427 Furong Road of Changsha, Changsha, Hunan, 410007, China.|Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410007, China.
authors.forename: Guofeng
authors.initials: G
authors.lastname: Xu
authors.affiliation: Clinical Laboratory, Hunan Provincial Second People's Hospital, 427 Furong Road of Changsha, Changsha, Hunan, 410007, China.|Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410007, China.
authors.forename: Junyu
authors.initials: J
authors.lastname: He
authors.affiliation: Clinical Medical School, Hunan University of Traditional Chinese Medicine, Changsha, Hunan, 410007, China.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Clinical Laboratory, Hunan Provincial Second People's Hospital, 427 Furong Road of Changsha, Changsha, Hunan, 410007, China.
authors.forename: Haowen
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Clinical Laboratory, Hunan Provincial Second People's Hospital, 427 Furong Road of Changsha, Changsha, Hunan, 410007, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000097:blood / Q000235:genetics* / Q000276:immunology; D000368:Aged; D000906:Antibodies / Q000097:blood; D014408:Biomarkers, Tumor / Q000097:blood; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D005260:Female; D018840:HSP70 Heat-Shock Proteins / Q000097:blood / Q000235:genetics* / Q000276:immunology; D006801:Humans; D056724:Immunity, Humoral; D008103:Liver Cirrhosis / Q000378:metabolism / Q000473:pathology; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008175:Lung Neoplasms / Q000175:diagnosis* / Q000378:metabolism / Q000473:pathology; D008297:Male; D008875:Middle Aged
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D000906:Antibodies; D014408:Biomarkers, Tumor; D018840:HSP70 Heat-Shock Proteins; C111047:SPAG9 protein, human
keywords: Auto-antibody; Heat shock protein 70; Hepatocellular carcinoma; Lung cancer; Sperm-associated antigen 9