title: Identification of novel Newcastle disease virus sub-genotype VII-(j) based on the fusion protein.
abstract: Newcastle disease virus (NDV) is believed to be the cause of fatal poultry disease worldwide. The fusion (F) protein plays a key role in virus pathogenesis, and it is also used for Newcastle disease virus classification. In this study, we determined the complete coding sequence of the F gene in new velogenic NDV isolates with an intravenous pathogenicity index (IVPI) of 1.8 and a mean death time (MDT) of 72 or 48 h. Complete sequences of the F genes of new Iranian isolates were amplified and sequenced in both directions. These isolates were compared to 195 nucleotide sequences from GenBank (available as of 07/17/2016). A phylogenetic tree was constructed for the F gene, using MEGA6 software with statistical analysis based on 500 bootstrap replicates. Evolutionary distances revealed that the new virulent isolates from Iran belonged to genotype VII in a new distinct sub-genotype named VII-(j). This new sub-genotype showed 3% divergence from genotype VIId. Recombination analysis showed that these new isolates were not recombinant NDVs.
authors.affiliation: Central Laboratory Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran. m.esmaelizad@rvsri.ac.ir.
authors.forename: Majid
authors.initials: M
authors.lastname: Esmaelizad
authors.affiliation: Central Laboratory Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran.
authors.forename: Vafa
authors.initials: V
authors.lastname: Mayahi
authors.affiliation: Central Laboratory Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Pashaei
authors.affiliation: Central Laboratory Department, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Goudarzi
mesh_terms: D000818:Animals; D002645:Chickens; D005838:Genotype; D007492:Iran; D009521:Newcastle Disease / Q000821:virology*; D009522:Newcastle disease virus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010802:Phylogeny; D011201:Poultry Diseases / Q000821:virology*; D014760:Viral Fusion Proteins / Q000235:genetics*
chemical_list: D014760:Viral Fusion Proteins