title: Upregulated Expression of TRIM32 Is Involved in Schwann Cell Differentiation, Migration and Neurite Outgrowth After Sciatic Nerve Crush.
abstract: Tripartite motif containing 32 (TRIM32), a member of the tripartite motif (TRIM) family, plays an indispensable role in myoblast proliferation. It also regulates neuron and skeletal muscle stem cell differentiation. Although it is of great importance, we know little about the roles of TRIM32 during peripheral nervous system injury. Here, we examined the dynamic changes of TRIM32 in acute sciatic nerve crush (SNC) model. After crush, TRIM32 rapidly increased and reached the climax at 1 week but then gradually declined to the normal level at 4 weeks post-injury. Meanwhile, we observed similar changes of Oct-6. What is more, we found co-localization of TRIM32 with S100 and Oct-6 in 1-week-injured tissues using double immunofluorescent staining. In further vitro experiments, enhancive expression of TRIM32 was detected during the process of cyclic adenosine monophosphate (cAMP)-induced Schwann cell differentiation and nerve growth factor (NGF)-induced PC12 cell neurite outgrowth. More interestingly, specific si-TRIM32-transfected RSC96 cells exhibited obvious reduction in the ability of migration. Taken together, we inferred that upregulated TRIM32 was not only involved in the differentiation and migration of Schwann cells but the neurite elongation after SNC.
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Nantong University, 19 Qixiu Road, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.|Department of Pathogen Biology, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Yonghua
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Wu
authors.affiliation: Department of Orthopaedics, Affiliated Jiangyin Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Huiguang
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Orthopaedics, Affiliated Jiangyin Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Zhengming
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Orthopaedics, Affiliated Mental Health Center of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Chi
authors.initials: C
authors.lastname: Sun
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Yuxi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Class 2, Grade 13, Clinical Medicine, Medical College, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Orthopaedics, Affiliated Hospital of Nantong University, Nantong University, Nantong, Jiangsu, 226001, People's Republic of China. wyouhua1516@163.com.
authors.forename: Youhua
authors.initials: Y
authors.lastname: Wang
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000502:physiology*; D002460:Cell Line; D002465:Cell Movement / Q000502:physiology*; D015870:Gene Expression; D008297:Male; D000071446:Neuronal Outgrowth / Q000502:physiology*; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000502:physiology*; D020426:Sciatic Neuropathy / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D014157:Transcription Factors / Q000096:biosynthesis* / Q000235:genetics; D000070584:Tripartite Motif Proteins / Q000096:biosynthesis* / Q000235:genetics; D044767:Ubiquitin-Protein Ligases / Q000096:biosynthesis* / Q000235:genetics; D015854:Up-Regulation / Q000502:physiology
chemical_list: D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; D044767:Ubiquitin-Protein Ligases; C000617052:trim32 protein, rat
keywords: Differentiation; Neurite outgrowth; PC12 cell; Schwann cell; Sciatic nerve crush; TRIM32