title: Succinyl-β-cyclodextrin modified gold biochip improved seroimmunological detection sensitivity for Lyme disease.
abstract: Cyclodextrin (CD) is a kind of cyclic oligosaccharides, which forms host-guest interactions with hydrophobic molecules and is widely applied in capillary electrophoresis and pharmaceutical engineering. In this study, we established a succinyl-β-CD modified gold biochip for improvement of seroimmunological detection sensitivity of Lyme disease. We found that the CD modified biochip platform presented a stronger affinity property for VlsE protein in conjugation with >0.000475 μg/mL of antigen immobilization concentration, which was sensitive enough for fluorescence based assay. Detection limit for anti-VlsE IgG antibody was 0.39 μg/mL. Specificity of VlsE assay on the succinyl-β-CD modified biochip was successfully confirmed by an immunological block assay. Furthermore, the correlation coefficient (R2) between the fluorescence values by the biochip and the OD values by ELISA assay was 0.904, indicating this biochip-based immunological assay might be a potential substitute with the ELISA-based approach. This biochip platform would be not suitable for loading of flagellin and OspC.
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ye
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, PR China.
authors.forename: Na-Li
authors.initials: NL
authors.lastname: Huang
authors.affiliation: Sektion Experimentelle Anästhesiologie, Universitätsklinikum Ulm, Ulm 89081, Germany.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Du
authors.affiliation: Sektion Experimentelle Anästhesiologie, Universitätsklinikum Ulm, Ulm 89081, Germany.
authors.forename: Marion
authors.initials: M
authors.lastname: Schneider
authors.affiliation: Department of Pathology, Anhui Medical University, Hefei 230032, PR China. Electronic address: weidong.du@ahmu.edu.cn.
authors.forename: Wei-Dong
authors.initials: WD
authors.lastname: Du
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial / Q000032:analysis* / Q000097:blood; D002648:Child; D002675:Child, Preschool; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006046:Gold; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007074:Immunoglobulin G; D007223:Infant; D008193:Lyme Disease / Q000175:diagnosis*; D008297:Male; D008875:Middle Aged; D040081:Protein Array Analysis; D012680:Sensitivity and Specificity; D055815:Young Adult; D047392:beta-Cyclodextrins
chemical_list: D000907:Antibodies, Bacterial; D000942:Antigens, Bacterial; D007074:Immunoglobulin G; D047392:beta-Cyclodextrins; D006046:Gold
keywords: Cyclodextrin; Enzyme-linked immunosorbent assay; Lyme disease; Protein biochip; Variable major protein-like sequence