title: Multicopper Laccase Mimicking Nanozymes with Nucleotides as Ligands.
abstract: Using nanomaterials to achieve functional enzyme mimics (nanozymes) is attractive for both applied and fundamental research. Laccases are multicopper oxidases highly important for biotechnology and environmental remediation. In this work, we report an exceptionally simple yet functional laccase mimic based on guanosine monophosphate (GMP) coordinated copper. It forms an amorphous metal-organic framework (MOF) material. The ratio of copper and GMP is 3:4 as determined by isothermal titration calorimetry. It has excellent laccase-like activity and converts a diverse range of phenol containing substrates such as hydroquinone, naphthol, catechol and epinephrine. Comparative work shows that the activity is originated from guanosine coordination instead of phosphate binding in GMP. Cu2+is required and cannot be substituted by other metal ions. At the same mass concentration, the Cu/GMP nanozyme has a higher Vmaxand similar Kmcompared to the protein laccase. To achieve the same catalytic efficiency, the cost of the Gu/GMP is ∼2400-fold lower than that of laccase. The Cu/GMP is much more stable at extreme pH, high salt, high temperature and for long-term storage. This is one of the first laccase-mimicking nanozymes, which will find important applications in analytical chemistry, environmental protection, and biotechnology.
authors.affiliation: State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Liang
authors.affiliation: State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Lin
authors.affiliation: Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Zijie
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Biwu
authors.initials: B
authors.lastname: Liu
authors.affiliation: State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Shuhui
authors.initials: S
authors.lastname: Jiang
authors.affiliation: State key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology , Beijing 100029, China.
authors.forename: Qipeng
authors.initials: Q
authors.lastname: Yuan
authors.affiliation: Department of Chemistry and Waterloo Institute for Nanotechnology, University of Waterloo , Waterloo, Ontario N2L 3G1, Canada.
authors.forename: Juewen
authors.identifier: http://orcid.org/0000-0001-5918-9336
authors.initials: J
authors.lastname: Liu
mesh_terms: D003300:Copper; D042845:Laccase; D008024:Ligands; D049329:Nanostructures; D009711:Nucleotides / Q000737:chemistry*
chemical_list: D008024:Ligands; D009711:Nucleotides; D003300:Copper; D042845:Laccase
keywords: copper; laccase; metal−organic frameworks; nanozymes; nucleotides