title: Copolymerization of recombinant Phascolopsis gouldii hemerythrin with human serum albumin for use in blood substitutes.
abstract: Hemerythrin is an oxygen-carrying protein found in marine invertebrates and may be a promising alternative to hemoglobin for use in blood substitutes, primarily due to its negligible peroxidative toxicity. Previous studies have shown that glutaraldehyde-induced copolymerization of hemoglobin with bovine serum albumin increases the half-life of the active oxy form of hemoglobin (i.e. decreases the auto-oxidation rate). Here, we describe a protocol for glutaraldehyde copolymerization of Hr with human serum albumin and the dioxygen-binding properties of the co-polymerized products. The copolymerization with HSA results in alteration of hemerythrin's dioxygen-binding properties in directions that may be favorable for use in blood substitutes.
authors.affiliation: a Department of Chemistry , "Babeş-Bolyai" University , Cluj-Napoca , Romania.
authors.forename: Mariann
authors.initials: M
authors.lastname: Arkosi
authors.affiliation: a Department of Chemistry , "Babeş-Bolyai" University , Cluj-Napoca , Romania.
authors.forename: Florina
authors.initials: F
authors.lastname: Scurtu
authors.affiliation: b Interdisciplinary Research Institute on Bio-Nano-Sciences, "Babeş-Bolyai" University , Cluj-Napoca , Romania.
authors.forename: Adriana
authors.initials: A
authors.lastname: Vulpoi
authors.affiliation: a Department of Chemistry , "Babeş-Bolyai" University , Cluj-Napoca , Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Silaghi-Dumitrescu
authors.affiliation: c Department of Chemistry , University of Texas at San Antonio, San Antonio , TX , USA.
authors.forename: Donald
authors.initials: D
authors.lastname: Kurtz
mesh_terms: D000818:Animals; D001802:Blood Substitutes / Q000138:chemical synthesis / Q000737:chemistry; D002417:Cattle; D006422:Hemerythrin / Q000737:chemistry* / Q000235:genetics; D006801:Humans; D011077:Polychaeta / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics; D012710:Serum Albumin, Bovine / Q000737:chemistry*
chemical_list: D001802:Blood Substitutes; D006422:Hemerythrin; D011994:Recombinant Proteins; D012710:Serum Albumin, Bovine
keywords: Hemerythrin; blood substitutes; hemoglobin; human serum albumin; oxygen transport