title: Modulatory effect of standardised amentoflavone isolated from Juniperus communis L. agianst Freund's adjuvant induced arthritis in rats (histopathological and X Ray anaysis).
abstract: Juniperus communis. L. is a shrub or small evergreen tree, native to Europe, South Asia, and North America, and belongs to family Cupressaceae. It has been used traditionally in unani system and in Swedish medicine as a decoction in inflammatory diseases. The main chemical constituents, which were reported in J. communis L. was α-pinene,, apigenin, sabinene, β-sitosterol, campesterol, limonene, Amentoflavone (AF), cupressuflavone, and many others.
  
  
  OBJECTIVE
  The aim of present study was to isolate the amentoflavone from the plant juniperus communis L. extracts and its protective effects against Freund's adjuvant induced arthritis in rats.
  
  
  METHODS
  Adjuvant arthritis was induced by an injection of 1mg heat killed Mycobacterium tuberculosis (CFA) into the left hind paw of rat by sub planter route (at day 0). The experiment was designed and modified as per method available in literature.
  
  
  RESULTS
  The study showed that at a dose of 40mg/kg of amentoflavone (AF) from methanolic extract of Juniperus Communis L. possessed potentially useful anti-arthritic activity as it gave a positive result in controlling inflammation in the adjuvant induced experimental model.
  
  
  CONCLUSIONS
  From the present experimental findings of both pharmacological and biochemical parameters observed, it had been concluded that at the doses of 20mg/kg and 40mg/kg of AF fraction from methanolic extract of Juniperus communis L. It possesses useful anti-arthritic activity since it gives a positive result in controlling inflammation in the adjuvant induced arthritic model in rats. The drug is a promising anti-arthritic agent of plant origin in the treatment of inflammatory disorders.
authors.affiliation: Dept of Pharmacology, Rayat Institute of Pharmacy, Railmajra, Distt. SBS. Nagar, Punjab 144533, India. Electronic address: souravh2008.123@rediffmail.com.
authors.forename: Souravh
authors.initials: S
authors.lastname: Bais
authors.affiliation: Rayat Institute of Pharmacy, Railmajra, Distt. SBS. Nagar, Punjab, 144533, India. Electronic address: abrolnaveena@gmail.com.
authors.forename: Naveena
authors.initials: N
authors.lastname: Abrol
authors.affiliation: Dept of Pharmacology, Rayat Institute of Pharmacy, Railmajra, Distt. SBS. Nagar, Punjab 144533, India. Electronic address: yashprashar@gmail.com.
authors.forename: Yash
authors.initials: Y
authors.lastname: Prashar
authors.affiliation: Rayat Institute of Pharmacy, Railmajra, Distt. SBS. Nagar, Punjab, 144533, India. Electronic address: renuparwal28790@gmail.com.
authors.forename: Renu
authors.initials: R
authors.lastname: Kumari
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000139:chemically induced* / Q000188:drug therapy*; D044946:Biflavonoids / Q000302:isolation & purification* / Q000494:pharmacology*; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology*; D007249:Inflammation / Q000188:drug therapy; D018653:Juniperus / Q000737:chemistry*; D008297:Male; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D014965:X-Rays
chemical_list: D044946:Biflavonoids; D010936:Plant Extracts; D005620:Freund's Adjuvant; C011164:amentoflavone
keywords: Amentoflavone; Freund’s adjuvant induced arthritis; Histopathology; Juniperus Communis L.; X- Ray