title: Improvement influenza HA2 DNA vaccine cellular and humoral immune responses with Mx bio adjuvant.
abstract: Immunization with DNA vaccines as a novel alternative to conventional vaccination strategy requires adjuvant for improving vaccine efficacy. The conserved immunogenic HA2 subunit, which harbors neutralizing epitopes is a promising vaccine candidate against influenza viruses. In this study, for the first time we explore the idea of using host interferon inducible Mx protein to increase the immunogenicity of HA2 H9N2 influenza DNA vaccine. The potency and safety of the Mx adjuvanted-HA2 vaccine was evaluated in BALB/c mice by different prime-boost strategies. To assess the effect of the vaccination on the virus clearance rate, mice were challenged with homologous influenza virus. Administration of the adjuvanted vaccine and boosting with the same regimen could effectively enhance both humoral and cellular immune responses in treated mice. These data demonstrated that Mx as host defense peptide can be potentiated for improving influenza vaccine efficacy.
authors.affiliation: Faculty of Veterinary Medicine, Tehran University, P.O. Box 14155-6453, Tehran, Iran; Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization, P.O. Box 31975-148, Karaj, Iran.
authors.forename: Sina
authors.initials: S
authors.lastname: Soleimani
authors.affiliation: Razi Vaccine & Serum Research Institute, Agricultural Research Education and Extension Organization, P.O. Box 31975-148, Karaj, Iran. Electronic address: s.shahsavandi@rvsri.ac.ir.
authors.forename: Shahla
authors.initials: S
authors.lastname: Shahsavandi
authors.affiliation: Faculty of Veterinary Medicine, Tehran University, P.O. Box 14155-6453, Tehran, Iran.
authors.forename: Omid
authors.initials: O
authors.lastname: Maddadgar
mesh_terms: D000818:Animals; D000914:Antibodies, Viral / Q000097:blood / Q000276:immunology; D017024:Chemotherapy, Adjuvant / Q000379:methods; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D007111:Immunity, Cellular / Q000187:drug effects / Q000276:immunology*; D056724:Immunity, Humoral / Q000187:drug effects / Q000276:immunology*; D007117:Immunization, Secondary / Q000379:methods; D053127:Influenza A Virus, H9N2 Subtype / Q000276:immunology; D007252:Influenza Vaccines / Q000008:administration & dosage / Q000276:immunology*; D008807:Mice, Inbred BALB C; D064547:Myxovirus Resistance Proteins / Q000008:administration & dosage / Q000276:immunology*; D009976:Orthomyxoviridae Infections / Q000276:immunology* / Q000517:prevention & control / Q000821:virology; D016896:Treatment Outcome; D014611:Vaccination / Q000379:methods; D019444:Vaccines, DNA / Q000008:administration & dosage / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D007252:Influenza Vaccines; D064547:Myxovirus Resistance Proteins; D019444:Vaccines, DNA
keywords: Bio adjuvant; HA2 subunit; Immune responses; Influenza; Mx