title: Potassium carbonate as a cation source for early-lactation dairy cows fed high-concentrate diets.
abstract: Previous studies reported that addition of K2CO3to high-concentrate diets improved milk fat synthesis, although the mechanism is yet to be established. The objective of the current experiment was to investigate the effects of dietary cation-anion difference (DCAD), cation source, and buffering ability of the mineral supplement on rumen biohydrogenation of fatty acids and production performance in dairy cows fed a high-concentrate diet. Thirty-five early-lactation Holstein cows (25 multiparous ruminally fistulated and 10 primiparous nonfistulated) were used in a randomized complete block design (7 blocks) with 33-d periods, including a 5-d pre-treatment collection period used as a covariate. Diets were (1) control, a basal diet [47% nonfibrous carbohydrates, DCAD (Na + K - Cl - S) = 65 mEq/kg of dry matter (DM)] containing 40% forage (including 60% corn silage) and 60% concentrate, (2) K2CO3(control + K2CO3, 1.8% of DM, DCAD = 326 mEq/kg of DM), (3) KHCO3(control + KHCO3, 2.6% of DM, DCAD = 324 mEq/kg of DM), (4) KCl (control + KCl, 2.0% of DM, DCAD = 64 mEq/kg of DM), and (5) Na2CO3(control + Na2CO3, 1.4% of DM, DCAD = 322 mEq/kg of DM). Pre-planned orthogonal contrasts were used to assess the effects of K2CO3(control vs. K2CO3), buffering ability (K2CO3vs. KHCO3), DCAD (K2CO3vs. KCl), and cation type (K2CO3vs. Na2CO3). Supplementing K2CO3in a high-concentrate diet did not improve milk fat yield or 4% fat-corrected milk yield. Milk fat concentration was greater in cows fed K2CO3compared with control (4.03 vs. 3.26%). Milk yield tended to decrease (34.5 vs. 38.8 kg/d) and lactose yield decreased in cows fed K2CO3as compared with KCl (1.64 vs. 1.87 kg/d). Milk fat concentration of trans-10 18:1 was increased when cows were fed Na2CO3as compared with K2CO3. A positive relationship was observed between concentrations of anteiso 15:0 and trans-10,cis-12 18:2 in milk fat from cows receiving K2CO3. Milk Na concentration was increased, whereas milk Cl was decreased with K2CO3as compared with KHCO3or KCl. A positive relationship was established between milk Cl concentration and milk yield (R2= 0.34) across all dietary treatments. Cation-anion difference (Na + K - Cl - S) in ruminal fluid was increased with K2CO3as compared with control or KCl. Blood pH tended to decrease in cows fed KCl compared with K2CO3. Our results suggest that mineral supplementation tends to affect milk and milk fat synthesis and that factors other than DCAD, potassium ion, or buffer ability may be implicated. The variations observed in mineral composition of milk suggest an allostatic process to maintain an ionic equilibrium in mammary epithelial cells in response to mineral composition of the diet.
authors.affiliation: Département des Sciences Animales, Université Laval, Québec, QC, Canada G1V 0A6.
authors.forename: A R
authors.initials: AR
authors.lastname: Alfonso-Avila
authors.affiliation: Département des Sciences Animales, Université Laval, Québec, QC, Canada G1V 0A6.
authors.forename: É
authors.initials: É
authors.lastname: Charbonneau
authors.affiliation: Département des Sciences Animales, Université Laval, Québec, QC, Canada G1V 0A6.
authors.forename: P Y
authors.initials: PY
authors.lastname: Chouinard
authors.affiliation: Québec Research and Development Centre, Agriculture and Agri-Food Canada, Québec, QC, Canada GIV 2J3.
authors.forename: G F
authors.initials: GF
authors.lastname: Tremblay
authors.affiliation: Département des Sciences Animales, Université Laval, Québec, QC, Canada G1V 0A6. Electronic address: rachel.gervais@fsaa.ulaval.ca.
authors.forename: R
authors.initials: R
authors.lastname: Gervais
mesh_terms: D000821:Animal Feed; D000818:Animals; D002412:Cations; D002417:Cattle; D004032:Diet / Q000662:veterinary; D005260:Female; D007774:Lactation; D008892:Milk / Q000737:chemistry
chemical_list: D002412:Cations
keywords: chlorine; dietary cation-anion difference; milk fat synthesis; potassium carbonate