title: Enzymes Beat Chemists in the Formation of an Unnatural Bond.
abstract: A fundamental difference? A heme protein known to catalyze electron transfers was engineered into an enzyme that catalyzes the formation of C-Si bonds with >99 % ee. The new enzyme uses diazoesters as carbene donors for the insertion of various silanes into the Si-H bond. This is the first reported organosilicon-producing enzyme.
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC, H3A 0B8, Canada.
authors.forename: Julie
authors.initials: J
authors.lastname: Ducharme
authors.affiliation: Department of Chemistry, McGill University, 801 Sherbrooke Street West, Montreal, QC, H3A 0B8, Canada.
authors.forename: Karine
authors.identifier: 0000-0003-0933-7956
authors.initials: K
authors.lastname: Auclair
mesh_terms: D002384:Catalysis; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D004798:Enzymes / Q000235:genetics* / Q000378:metabolism*; D004952:Esters / Q000378:metabolism; D006418:Heme / Q000235:genetics* / Q000378:metabolism; D017646:Organosilicon Compounds / Q000378:metabolism*; D015202:Protein Engineering; D012821:Silanes / Q000737:chemistry*
chemical_list: D004798:Enzymes; D004952:Esters; D017646:Organosilicon Compounds; D012821:Silanes; D006418:Heme
keywords: C-Si bonds; enzyme engineering; heme proteins; mutagenesis; organosilicon