title: Differential expression of miRNAs and associated gene targets in grapevine leafroll-associated virus 3-infected plants.
abstract: MicroRNAs (miRNAs) are a class of endogenous small non-coding RNAs (sRNA) that play an essential role in the regulation of target mRNAs expressed during plant development and in response to stress. MicroRNA expression profiling has helped to identify miRNAs that regulate a range of processes, including the plant's defence response to pathogens. In this study, differential miRNA expression in own-rooted Vitis vinifera cv. Cabernet Sauvignon plants infected with grapevine leafroll-associated virus 3 was investigated with microarrays and next-generation sequencing (NGS) of sRNA and mRNA. These high-throughput approaches identified several differentially expressed miRNAs. Four miRNAs, identified by both approaches, were validated by stemloop RT-PCRs. Three of the predicted targets of the differentially expressed miRNAs were also differentially expressed in the transcriptome data of infected plants, and were validated by RT-qPCR. Identification of these miRNAs and their targets can lead to a better understanding of host-pathogen interactions involved in grapevine leafroll disease and the identification of possible targets for virus resistance.
authors.affiliation: Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
authors.forename: Rachelle
authors.initials: R
authors.lastname: Bester
authors.affiliation: Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa.
authors.forename: Johan T
authors.initials: JT
authors.lastname: Burger
authors.affiliation: Department of Genetics, Stellenbosch University, Private Bag X1, Matieland, 7602, South Africa. hjmaree@sun.ac.za.|Agricultural Research Council, Infruitec-Nietvoorbij (The Fruit, Vine and Wine Institute), Private Bag X5026, Stellenbosch, 7599, South Africa. hjmaree@sun.ac.za.
authors.forename: Hans J
authors.initials: HJ
authors.lastname: Maree
mesh_terms: D029381:Closteroviridae / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D018506:Gene Expression Regulation, Plant; D054884:Host-Pathogen Interactions; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D010935:Plant Diseases / Q000235:genetics* / Q000821:virology; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D018749:RNA, Plant / Q000235:genetics / Q000378:metabolism*; D027843:Vitis / Q000235:genetics / Q000378:metabolism* / Q000821:virology
chemical_list: D035683:MicroRNAs; D010940:Plant Proteins; D018749:RNA, Plant