title: Change in Glutenin Macropolymer Secondary Structure in Wheat Sourdough Fermentation by FTIR.
abstract: Wheat sourdough was prepared by fermentation with Lactobacillus plantarum M616 and yeast in the present study. The change in secondary structure of glutenin macropolymer (GMP) in wheat sourdough fermentation for 4 and 12 h was determined using Fourier transform infrared spectroscopy, and then the resultant spectra were Fourier self-deconvoluted of the amide I band in the region from 1600 to 1700 cm-1. Significant different spectra especially in the amide I band for GMP from sourdough fermented with L. plantarum M616 (SL) and with L. plantarum M616 and yeast (SLY) were found in respect of control dough (CK), dough with acids (SA), and sourdough fermented with yeast (SY) at 4 and 12 h of fermentation. The loss of α-helix structure in SL, SLY, and SA samples was noticed during fermentation. Compared with CK and SY, SL, SLY, and SA samples showed significant decrease (p < 0.05) in the relative areas of α-helix at the same stage of fermentation. In addition, β-turns in SL sourdough decrease, and the relative areas of random coil increase significantly (p < 0.05). These changes in the secondary structure mean that the flexibility of glutenin macropolymer in sourdough increases and it makes GMP degradation easier during fermentation. The modified secondary structure of GMP makes more sensitive to proteolysis by means of cereal enzymes.
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China. jinshuiw@163.com.
authors.forename: Jinshui
authors.initials: J
authors.lastname: Wang
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Yue
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
authors.forename: Tiantian
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
authors.forename: Zhenlei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China.
authors.forename: Changfu
authors.initials: C
authors.lastname: Zhang
mesh_terms: D005285:Fermentation / Q000502:physiology*; D005983:Glutens / Q000737:chemistry* / Q000378:metabolism*; D048191:Lactiplantibacillus plantarum / Q000378:metabolism; D017550:Spectroscopy, Fourier Transform Infrared; D014908:Triticum / Q000378:metabolism*
chemical_list: D005983:Glutens; C037302:glutenin
keywords: Fermentation; Fourier transform infrared spectroscopy (FTIR); Glutenin macropolymer; Secondary structure; Sourdough