title: Development of a vivo rabbit ligated intestinal Loop Model for HCMV infection.
abstract: BACKGROUND
  Human Cytomegalovirus (HCMV) infections can be found throughout the body, especially in epithelial tissue. Animal model was established by inoculation of HCMV (strain AD-169) or coinoculation with Hepatitis E virus (HEV) into the ligated sacculus rotundus and vermiform appendix in living rabbits. The specimens were collected from animals sacrificed 1 and a half hours after infection.
  
  
  RESULTS
  The virus was found to be capable of reproducing in these specimens through RT-PCR and Western-blot. Severe inflammation damage was found in HCMV-infected tissue. The viral protein could be detected in high amounts in the mucosal epithelium and lamina propria by immunohistochemistry and immunofluorescense. Moreover, there are strong positive signals in lymphocytes, macrophages, and lymphoid follicles. Quantitative statistics indicate that lymphocytes among epithlium cells increased significantly in viral infection groups.
  
  
  CONCLUSIONS
  The results showed that HCMV or HEV + HCMV can efficiently infect in rabbits by vivo ligated intestine loop inoculation. The present study successfully developed an infective modelin vivorabbit ligated intestinal Loop for HCMV pathogenesis study. This rabbit model can be helpful for understanding modulation of the gut immune system with HCMV infection.
authors.affiliation: National animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Jin
authors.initials: J
authors.lastname: Tang
authors.affiliation: Laboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Qiaoxing
authors.initials: Q
authors.lastname: Wu
authors.affiliation: National animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Xinming
authors.initials: X
authors.lastname: Tang
authors.affiliation: Laboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Ruihan
authors.initials: R
authors.lastname: Shi
authors.affiliation: National animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Jingxia
authors.initials: J
authors.lastname: Suo
authors.affiliation: National animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Guangping
authors.initials: G
authors.lastname: Huang
authors.affiliation: Laboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Junqing
authors.initials: J
authors.lastname: An
authors.affiliation: Laboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Jingyuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Laboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Jinling
authors.initials: J
authors.lastname: Yang
authors.affiliation: Laboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Wenzhuo
authors.initials: W
authors.lastname: Hao
authors.affiliation: Laboratory of Animal Pathology & Public Health, College of Veterinary Medicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Ruiping
authors.initials: R
authors.lastname: She
authors.affiliation: National animal protozoa laboratory,College of VeterinaryMedicine, China Agricultural University, Beijing, 100193 China.
authors.forename: Xun
authors.initials: X
authors.lastname: Suo
keywords: HCMV; HEV; Immunohistochemistry and confocal immunofluorescence; Inoculating ligated intestine in vivo; Pathological lesion; Rabbit sacculus rotundus; Vermiform appendix