title: Restoration of density of interstitial cells of Cajal in the jejunum of diabetic rats after quercetin supplementation.
abstract: BACKGROUND
  Interstitial cells of Cajal (ICC) are required for normal motility in the gastrointestinal tract. Depletion of ICC has been associated with diabetic gastroenteropathy.
  
  
  OBJECTIVE
  To determine the effect of quercertin supplementation on anoctamin-1 (Ano1) immunoreactive ICC in the myenteric region (ICC-MY) and deep muscular plexus (ICC-DMP) in the jejunum of diabetic rats.
  
  
  METHODS
  Thirty-two 90-day-old male Wistar rats were distributed into the following groups: normoglycemic (C), normoglycemic supplemented with quercetin (CQ; 40 mg daily), diabetic (D), and diabetic supplemented with quercetin (DQ; 40 mg daily). Diabetes was induced by streptozotocin injection. After 120 days, preparations of the jejunal muscular and submucosal layers were immunostained for Ano1 to visualize ICC. Evaluation of the immunofluorescence intensity as well as density of ICC was performed.
  
  
  RESULTS
  The density of ICC-MY was 46% lower in group D compared to group C (p < 0.01); ICC-DMP were reduced by 37% (p > 0.05). After quercertin treatment, the densities of ICC-MY were significantly higher in the DQ group compared to group D (ICC-MY: 58%, p < 0.05). Supplementation with quercetin in normoglycemic animals (CQ) compared with group C did not significantly change the ICC density (p > 0.05).
  
  
  CONCLUSIONS
  In STZ-treated diabetic rats, diabetes promoted a reduction in the density of jejunal ICC-MY with no significant effect on ICC-DMP. Supplementation with quercetin (DQ) appeared to protect ICC-MY from depletion in diabetes possibly due to its antioxidant action.
authors.affiliation: Morphological Science, State University of Maringá, Brazil.
authors.forename: Flávia Cristina
authors.initials: FC
authors.lastname: Vieira Frez
authors.affiliation: Department of Morphological Sciences, State University of Maringá.
authors.forename: Juliana Vanessa
authors.initials: JV
authors.lastname: Martins Colombo Perles
authors.affiliation: Mayo Clinic.
authors.forename: David
authors.initials: D
authors.lastname: Robert Linden
authors.affiliation: Physiology and Biomedical Engineering and Enteric, Mayo Clinic, USA.
authors.forename: Simon John
authors.initials: SJ
authors.lastname: Gibbons
authors.affiliation: Universidade Estadual de Maringá.
authors.forename: Heber
authors.initials: H
authors.lastname: Amilcar Martins
authors.affiliation: Morphological Science, Universidade Estadual de Maringá, Brazil.
authors.forename: Débora
authors.initials: D
authors.lastname: Almeida Brito Romualdo
authors.affiliation: Department of Morphological Sciences, State University of Maringá.
authors.forename: Sara Raquel
authors.initials: SR
authors.lastname: de Souza
authors.affiliation: Universidade Estadual de Maringá.
authors.forename: Gleison
authors.initials: G
authors.lastname: Daion Piovezana Bossolani
authors.affiliation: Morphological Sciences, State University of Maringá, Brazil.
authors.forename: Jacqueline Nelisis
authors.initials: JN
authors.lastname: Zanoni
mesh_terms: D000818:Animals; D000075369:Anoctamin-1 / Q000378:metabolism; D000975:Antioxidants / Q000627:therapeutic use*; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000473:pathology; D019587:Dietary Supplements; D056885:Interstitial Cells of Cajal / Q000187:drug effects* / Q000473:pathology; D007583:Jejunum / Q000187:drug effects / Q000473:pathology*; D008297:Male; D011794:Quercetin / Q000627:therapeutic use*; D051381:Rats; D017208:Rats, Wistar
chemical_list: C552942:ANO1 protein, rat; D000075369:Anoctamin-1; D000975:Antioxidants; D011794:Quercetin