title: Trigeminal Receptor Study of High-Intensity Cooling Agents.
abstract: Compounds capable of providing trigeminal effects are important to the flavor industry. Cooling agents activate a class of transient receptor potential (TRP) channels. The objective of this study was to evaluate the effect of 18 cooling agents by high-throughput screening through TRPM-8 and TRPA-1 channels. These coolants were further evaluated by a sensory analysis panel in a sucrose solution testing for a perceived activity. The results reveal that WS-5 activates both TRPA-1 and TRPM-8 receptor channels; WS-3 actives primarily TRPM-8, whereas FEMA-4557 activates the TRPA-1 channel. Sensory evaluation shows that for the same concentration in aqueous solution, the cooling intensity follows the order WS-5 > WS-3 > FEMA-4557 > WS-23. Human sensory perception of cooling intensity correlates better for TRPM-8 than for TRPA-1.
authors.affiliation: Wrigley, a Subsidiary of Mars Inc. , Chicago , Illinois 60642 , United States.
authors.forename: Sonya
authors.initials: S
authors.lastname: Johnson
authors.affiliation: Wrigley, a Subsidiary of Mars Inc. , Chicago , Illinois 60642 , United States.
authors.forename: Minmin
authors.initials: M
authors.lastname: Tian
authors.affiliation: Wrigley, a Subsidiary of Mars Inc. , Chicago , Illinois 60642 , United States.
authors.forename: Gloria
authors.initials: G
authors.lastname: Sheldon
authors.affiliation: Wrigley, a Subsidiary of Mars Inc. , Chicago , Illinois 60642 , United States.
authors.forename: Eric
authors.initials: E
authors.lastname: Dowd
mesh_terms: D003080:Cold Temperature; D005260:Female; D006801:Humans; D007700:Kinetics; D008297:Male; D000074025:TRPA1 Cation Channel / Q000737:chemistry / Q000378:metabolism*; D050053:TRPM Cation Channels / Q000737:chemistry / Q000378:metabolism*; D055697:Taste Perception
chemical_list: D000074025:TRPA1 Cation Channel; C473350:TRPA1 protein, human; D050053:TRPM Cation Channels; C425966:TRPM8 protein, human
keywords: TRPA-1; TRPM-8; chewing gum and mints; cooling agent; trigeminal receptor