title: [Effect of Human Umbilical Cord-derived Mesenchymal Stem Cells on Proliferation and Differentiation of Leukemia Cells].
abstract: OBJECTIVE
  To investigate the effect of human umbilical cord-derived mesenchymal stem cells(HUC-MSC) on the proliferation and differentiation of NB4 treated with all-trans retinoid acid (ATRA) and its underlining mechanisms .
  
  
  METHODS
  Human umbilical cord mesenchymal stem cells were isolated from umbilical cord of newborns. Co-culture system was established by HUC-MSC and NB4 in vitro. The experiment was divided into 4 groups: NB4 group (NB4 cells alone) , NM group (NB4 cells co-cultured with HUC-MSC) , NA group (NB4 cells treated with ATRA) , NMA group (NB4 cells co-cultured with HUC-MSC and treated with ATRA) . NB4 cells were counted by a microscopy, NB4 proliferation was monitored by CCK-8 assay, NB4 differentiation was assessed by Wright ' s staining and nitroblue tetrazolium reduction test. IL-6 levels in the culture supernatant of different groups were tested by ELISA kit. Quantitative PCR was used to detect the transcription level of CDKN1A, CCND1 and Survivin.
  
  
  RESULTS
  NB4 and HUC-MSC in the co-culturing systems were in good condition with a slight repression of NB4 proliferation by HUC-MSC. HUC-MSC could collaborate with ATRA to induce significant NB4 differentiation. Consistent with this finding, IL-6 expression levels of co-cultured groups were remarkably higher than that in any other groups or the group of HUC-MSC alone. The quantitative PCR analysis showed that the levels of CDKN1A and CCND1 mRNA expression were increased or decreased respectively in the co-cultured groups.
  
  
  CONCLUSIONS
  HUC-MSC co-culture can reduce proliferation but promote the differentiation of NB4 cells, suggesting that this effect may be closely related with the secretion of IL-6 which can affect the expression of some factors in vitro.
authors.affiliation: Weifang Medical University,Weifang 261042, Shandong Province, China.
authors.forename: Xiao-Huan
authors.initials: XH
authors.lastname: Ma
authors.affiliation: Weifang Medical University,Weifang 261042, Shandong Province, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Interventional Blood Vessel, The Fourth Affiliated Hospital of Harbin Medical University, Harbin 210013, Heilongjiang Province, China.
authors.forename: Chang-Yong
authors.initials: CY
authors.lastname: Zou
authors.affiliation: Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261042, Shandong Province, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261042, Shandong Province, China.
authors.forename: Zhan-Ju
authors.initials: ZJ
authors.lastname: Wang
authors.affiliation: Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261042, Shandong Province, China.
authors.forename: Hai-Ying
authors.initials: HY
authors.lastname: Wang
authors.affiliation: Weifang Medical University,Weifang 261042, Shandong Province, China.
authors.forename: Yu-Fen
authors.initials: YF
authors.lastname: Wang
authors.affiliation: Laboratory for Stem Cell and Regenerative Medicine, Affiliated Hospital of Weifang Medical University, Weifang 261042, Shandong Province, China. E-mail:huzhenbo@wfmc.edu.cn.
authors.forename: Zhen-Bo
authors.initials: ZB
authors.lastname: Hu
mesh_terms: D002454:Cell Differentiation; D049109:Cell Proliferation; D018920:Coculture Techniques; D050759:Cyclin-Dependent Kinase Inhibitor p21; D006801:Humans; D007938:Leukemia; D059630:Mesenchymal Stem Cells; D014470:Umbilical Cord
chemical_list: D050759:Cyclin-Dependent Kinase Inhibitor p21