title: Flow cytometric data analysis of circulating progenitor cell stability.
abstract: A recent publication by Mekonnen et al. demonstrated that among women with non-obstructive coronary artery disease, higher levels of circulating progenitor cells in the blood (CPC), were associated with impaired coronary flow reserve [1]. We performed a quality control assessment of the stability of circulating blood progenitor cells in blood samples stored at 4 °C, to determine the time period during which blood samples can be analyzed and yield consistent data for progenitor cell content. Healthy volunteers (n=6) were recruited and underwent phlebotomy, and blood was stored in EDTA tubes at 4 °C. Flow cytometry was performed to quantitate progenitor cell subsets at 0-4 h, 24 h, and 48 h post phlebotomy. All processed samples were fixed with 1% Paraformaldehyde and 1,000,000 total data events were collected. We found no significant differences in PC data for both CD34+ (P=0.68 for one-way ANOVA) and CD34+/CD133+ (P=0.74 for one-way ANOVA).
authors.affiliation: Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Ernestine A
authors.initials: EA
authors.lastname: Mahar
authors.affiliation: Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Liping
authors.initials: L
authors.lastname: Mou
authors.affiliation: Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Salim S
authors.initials: SS
authors.lastname: Hayek
authors.affiliation: Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Arshed A
authors.initials: AA
authors.lastname: Quyyumi
authors.affiliation: Emory University School of Medicine, Atlanta, GA, USA.
authors.forename: Edmund K
authors.initials: EK
authors.lastname: Waller