title: Production of a generic microbial feedstock for lignocellulose biorefineries through sequential bioprocessing.
abstract: Lignocellulosic materials, mostly from agricultural and forestry residues, provide a potential renewable resource for sustainable biorefineries. Reducing sugars can be produced only after a pre-treatment stage, which normally involves chemicals but can be biological. In this case, two steps are usually necessary: solid-state cultivation of fungi for deconstruction, followed by enzymatic hydrolysis using cellulolytic enzymes. In this research, the utilisation of solid-state bioprocessing using the fungus Trichoderma longibrachiatum was implemented as a simultaneous microbial pretreatment and in-situ enzyme production method for fungal autolysis and further enzyme hydrolysis of fermented solids. Suspending the fermented solids in water at 50°C led to the highest hydrolysis yields of 226mg/g reducing sugar and 7.7mg/g free amino nitrogen (FAN). The resultant feedstock was shown to be suitable for the production of various products including ethanol.
authors.affiliation: School of Chemical Engineering and Analytical Science, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom.
authors.forename: Chen-Wei
authors.initials: CW
authors.lastname: Chang
authors.affiliation: School of Chemical Engineering and Analytical Science, The University of Manchester, Oxford Road, Manchester M13 9PL, United Kingdom. Electronic address: colin.webb@manchester.ac.uk.
authors.forename: Colin
authors.initials: C
authors.lastname: Webb
mesh_terms: D019149:Bioreactors / Q000382:microbiology*; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry*; D014242:Trichoderma / Q000378:metabolism*
chemical_list: C036909:lignocellulose; D008031:Lignin
keywords: Generic microbial feedstock; In-situ enzyme hydrolysis; Solid-state bioprocessing; Soybean hull; Sugarcane bagasse; Trichoderma longibrachiatum