title: [QuEChERS pretreatment method for determination of organophosphate pesticide and raticide in whole blood].
abstract: Objective:To investigate the QuEChERS pretreatment method combined with on-line gel permeation chromatography-gas chromatography/mass spectrometry (GPC-GC/MS) for the determination of organophosphate pesticide and raticide in whole blood.Methods:The samples were pretreated with QuEChERS. A total of 1 ml acetonitrile was added to 1 ml whole blood sample for extraction, and 0.5 g sodium chloride was added. The mixture was oscillated for 15 seconds at a temperature of 4° C and centrifuged at a high speed of 12 000 r/min for 10 minutes. The supernatant was transferred to a centrifuge tube, the dispersive absorbent was used to remove the interfering matrix, and then GPC-GC/MS was used for analysis.Results:Organophosphate pesticide and raticide had a good linear relationship within the range of 0.1~0.5 mg/L, and the correlation coefficient was 0.997 7~1.000 0. The limit of quantitation of this method was 0.005~0.050 mg/L, and the recovery rate was 43.11%~106.68%, and the relative standard deviation of most drugs was <10%.Conclusion:The pretreatment method is simple, time-saving, and highly automated and has the features of low detection limit, high accuracy, and good accuracy. It can meet the requirements of rapid and accurate screening of organophosphate pesticides and raticide.
authors.affiliation: Department of Occupational Disease and Toxication, Chaoyang Hospital Affiliated to the Capital University of Medical Science, Beijing 100020, China.
authors.forename: F
authors.initials: F
authors.lastname: Dong
authors.forename: H L
authors.initials: HL
authors.lastname: Li
authors.forename: J
authors.initials: J
authors.lastname: Ma
authors.forename: B
authors.initials: B
authors.lastname: Zhou
authors.forename: Y
authors.initials: Y
authors.lastname: Yu
mesh_terms: D000097:Acetonitriles; D008401:Gas Chromatography-Mass Spectrometry; D007306:Insecticides; D057230:Limit of Detection; D010755:Organophosphates / Q000032:analysis*; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; D053719:Tandem Mass Spectrometry
chemical_list: D000097:Acetonitriles; D007306:Insecticides; D010755:Organophosphates; D009943:Organophosphorus Compounds; D010573:Pesticide Residues; C032159:acetonitrile