title: Identification, introgression, and validation of fruit volatile QTLs from a red-fruited wild tomato species.
abstract: Volatile organic compounds (VOCs) are major determinants of fruit flavor, a primary objective in tomato breeding. A recombinant inbred line (RIL) population consisting of 169 lines derived from a cross between Solanum lycopersicum and a red-fruited wild tomato species Solanum pimpinellifolium accession (SP) was characterized for VOCs in three different seasons. Correlation and hierarchical cluster analyses were performed on the 52 VOCs identified, providing a tool for the putative assignation of individual compounds to metabolic pathways. Quantitative trait locus (QTL) analysis, based on a genetic linkage map comprising 297 single nucleotide polymorphisms (SNPs), revealed 102 QTLs (75% not described previously) corresponding to 39 different VOCs. The SP alleles exerted a positive effect on most of the underlying apocarotenoid volatile QTLs-regarded as desirable for liking tomato-indicating that alleles inherited from SP are a valuable resource for flavor breeding. An introgression line (IL) population developed from the same parental genotypes provided 12 ILs carrying a single SP introgression and covering 85 VOC QTLs, which were characterized at three locations. The results showed that almost half of the QTLs previously identified in the RILs maintained their effect in an IL form, reinforcing the value of these QTLs for flavor/aroma breeding in cultivated tomato.
authors.affiliation: CSIC-Universidad Politécnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: José L
authors.initials: JL
authors.lastname: Rambla
authors.affiliation: CSIC-Universidad Politécnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: Aurora
authors.initials: A
authors.lastname: Medina
authors.affiliation: CSIC-Universidad Politécnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: Asun
authors.initials: A
authors.lastname: Fernández-Del-Carmen
authors.affiliation: CSIC-Universidad Politécnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: Walter
authors.initials: W
authors.lastname: Barrantes
authors.affiliation: National Research Council of Italy, Institute of Biosciences and Bioresources (CNR-IBBR), Research Division Portici, Via Università 133, Portici (Naples), Italy.
authors.forename: Silvana
authors.initials: S
authors.lastname: Grandillo
authors.affiliation: National Research Council of Italy, Institute of Biosciences and Bioresources (CNR-IBBR), Research Division Portici, Via Università 133, Portici (Naples), Italy.
authors.forename: Maria
authors.initials: M
authors.lastname: Cammareri
authors.affiliation: CSIC-Universidad de Málaga, Instituto de Hortofruticultura Subtropical y Mediterránea, Algarrobo Costa, Málaga, Spain.
authors.forename: Gloria
authors.initials: G
authors.lastname: López-Casado
authors.affiliation: CSIC-Universidad Politécnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Rodrigo
authors.affiliation: Departamento de Biología Aplicada, EPSO-UMH. Ctra, Beniel Km 3,2, Orihuela, Alicante, Spain.
authors.forename: Arancha
authors.initials: A
authors.lastname: Alonso
authors.affiliation: Departamento de Biología Aplicada, EPSO-UMH. Ctra, Beniel Km 3,2, Orihuela, Alicante, Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: García-Martínez
authors.affiliation: Universidad Politécnica de Valencia, Centro de Ecología Química Agrícola, Instituto Agroforestal Mediterráneo, Valencia, Spain.
authors.forename: Jaime
authors.initials: J
authors.lastname: Primo
authors.affiliation: Departamento de Biología Aplicada, EPSO-UMH. Ctra, Beniel Km 3,2, Orihuela, Alicante, Spain.
authors.forename: Juan J
authors.initials: JJ
authors.lastname: Ruiz
authors.affiliation: CSIC-Universidad de Málaga, Instituto de Hortofruticultura Subtropical y Mediterránea, Algarrobo Costa, Málaga, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Fernández-Muñoz
authors.affiliation: CSIC-Universidad Politécnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: Monforte
authors.affiliation: CSIC-Universidad Politécnica de Valencia, Instituto de Biología Molecular y Celular de Plantas, Valencia, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Granell
mesh_terms: D005638:Fruit / Q000737:chemistry / Q000378:metabolism; D017343:Genes, Plant; D006824:Hybridization, Genetic; D040641:Quantitative Trait Loci; D032322:Solanum / Q000235:genetics* / Q000378:metabolism*; D055549:Volatile Organic Compounds / Q000737:chemistry / Q000378:metabolism*
chemical_list: D055549:Volatile Organic Compounds
keywords: Fruit flavor; SolCap tomato SNP array; Solanum habrochaites; Solanum pimpinellifolium; introgression lines (ILs); quantitative trait loci (QTLs); recombinant inbred lines (RILs); tomato; volatiles