title: A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method.
abstract: BACKGROUND
  In cell-based therapies,in vitrostudies on biomimetic cell-scaffold constructs can facilitate the determination of the cell dose, a key factor in guaranteeing the effectiveness of the treatment. However, highly porous scaffolds do not allow a nondestructive evaluation of the cell number. Our objective was to develop a nondestructive method for human mesenchymal stem cells dose evaluation in a highly porous scaffold for bone regeneration.
  Proliferation trend of human mesenchymal stem cells on Biocoral®scaffolds was measured by a resazurin-based assay here optimized for 3D cultures. The method allows to noninvasively follow the cell proliferation on biocorals over 3 weeks with very high reproducibility.
  
  
  CONCLUSIONS
  This reliable method could be a powerful tool in cell-based therapies for cell dose determination.
authors.affiliation: INRIM - Istituto Nazionale di Ricerca Metrologica, Strada delle Cacce, 91 - 10135, Torino, Italy.
authors.forename: Carla
authors.initials: C
authors.lastname: Divieto
authors.affiliation: INRIM - Istituto Nazionale di Ricerca Metrologica, Strada delle Cacce, 91 - 10135, Torino, Italy.
authors.forename: Maria Paola
authors.initials: MP
authors.lastname: Sassi
keywords: Biocoral®; cell dose; cell number; cell proliferation; hMSCs; nondestructive methods; porous scaffolds