title: Synthesis of amine-functionalized Fe3O4@C nanoparticles for laccase immobilization.
abstract: Amine-functionalized Fe3O4@C nanoparticles with an average diameter of 266nm have been successfully synthesized by a solvothermal reduction method. The structure, morphology and properties of the Fe3O4@C nanoparticles were investigated through different analytical tools. Due to the magnetic nature and the presence of amine-functionalized groups, the as-prepared Fe3O4@C nanoparticles were employed as magnetic carriers for laccase immobilization. The results indicated that the loading of laccase onto the amine-functionalized Fe3O4@C nanoparticles was approximately 195mg/g. In comparison with free laccase, the pH, operational and storage stabilities of the immobilized laccase were significantly improved. After 10 consecutive operations, the immobilized laccase retained above 60% residual activity. The removal rates of o-phenylenediamine (OPD) by immobilized laccase were 88%, which were much higher than that of free laccase (50%). It hoped that the amine-functionalized Fe3O4@C nanoparticles may find an application in biotechnology and catalysis.
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Jiahong
authors.initials: J
authors.lastname: Lin
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Wen
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China. Electronic address: chens1968@hotmail.com.
authors.forename: Shi
authors.initials: S
authors.lastname: Chen
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Xueyi
authors.initials: X
authors.lastname: Le
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Xiaohua
authors.initials: X
authors.lastname: Zhou
authors.affiliation: College of Materials and Energy, South China Agricultural University, Guangzhou, 510642, China.
authors.forename: Limei
authors.initials: L
authors.lastname: Huang
mesh_terms: D000588:Amines / Q000737:chemistry*; D055162:Biocatalysis; D002244:Carbon / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic; D004800:Enzymes, Immobilized / Q000737:chemistry* / Q000378:metabolism; D042845:Laccase / Q000737:chemistry* / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D010655:Phenylenediamines / Q000378:metabolism; D055454:Trametes / Q000201:enzymology
chemical_list: D000588:Amines; D004800:Enzymes, Immobilized; D058185:Magnetite Nanoparticles; D010655:Phenylenediamines; D002244:Carbon; D042845:Laccase
keywords: Fe(3)O(4)@C nanoparticles; Laccase immobilization; O-phenylenediamine