title: Outlook on optimizing ultramicroscopy imaging technique through optical characterization.
abstract: Here, we present an optically optimized system for static ultramicroscopy imaging technique. The unit for generating an ultra-thin light sheet employs aspheric and meso-optical elements (meso-aspheric system). An analytical as well as an experimental comparison between the light sheet produced by the standard system (using a rectangular slit aperture and one cylindrical lens) and the one produced by our latest optimized system, which converts a symmetrical Gaussian beam into an ultra-thin light sheet is presented. Using the new light sheet in combination with our objective equipped with a modulator unit to compensate the refractive index mismatch between air and mediums with indices of 1.45-1.56, we present high resolution images of various biological samples that were chemically cleared using different methods. They demonstrate a marked improvement in quality, contrast and resolution.
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Saiedeh
authors.initials: S
authors.lastname: Saghafi
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Klaus
authors.initials: K
authors.lastname: Becker
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Christian
authors.initials: C
authors.lastname: Hahn
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Marko
authors.initials: M
authors.lastname: Pende
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Nina
authors.initials: N
authors.lastname: Jährling
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Martina
authors.initials: M
authors.lastname: Wanis
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Inna
authors.initials: I
authors.lastname: Sabdyusheva-Litschauer
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Massih
authors.initials: M
authors.lastname: Foroughipour
authors.affiliation: Pathologie Hamburg-West, Histology Diagnostics Department, Lornsenstraße 4, Hamburg, 22767, Germany.
authors.forename: Axel
authors.initials: A
authors.lastname: Niendorf
authors.affiliation: Department of Bioelectronics, FKE, Vienna University Technology, Vienna, Austria.|Center for Brain Research, Medical University of Vienna, Vienna, Austria.
authors.forename: Hans-Ulrich
authors.initials: HU
authors.lastname: Dodt
keywords: Gaussian beam; light sheet microscopy; optical parameters characterization; uniform beam profile