title: HMGCR inhibits the early stage of PCV2 infection, while PKC enhances the infection at the late stage.
abstract: Porcine circovirus type 2 (PCV2) is the smallest DNA virus, which causes porcine circovirus diseases and porcine circovirus-associated diseases (PCVD/PCVAD). Due the small size of viral genomic DNA, PCV2 replication predominantly relies on the host factors. In this study, effects of PKC and HMGCR on PCV2 infection were evaluated using real time PCR and western blot. We found that PKC and HMGCR participated in different stages of PCV2 infection. HMGCR works on the early stage of the infection to inhibit the virus infection, while PKC enhances the infection at the late stage. Furthermore, PKC enhances PCV2 replication by activating JNK1/2 and inactivating HMGCR via regulating phosphorylation of these two proteins, while HMGCR can suppress phosphorylation of JNK1/2. The results in the present study will provide new sights in the pathogenesis of PCV2 infection, as well as interactions between host factors during PCV2 infection.
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Teng
authors.initials: T
authors.lastname: Ma
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Xinrong
authors.initials: X
authors.lastname: Chen
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Hongsheng
authors.initials: H
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Liu
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ouyang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Zhiyuan
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Fuwang
authors.initials: F
authors.lastname: Chen
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China.
authors.forename: Daxin
authors.initials: D
authors.lastname: Pang
authors.affiliation: The Laboratory Animal Center, The Academic of Military Medical Sciences, Beijing 100071, P.R. China.
authors.forename: Jieying
authors.initials: J
authors.lastname: Bai
authors.affiliation: Jilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun, Jilin 130062, P.R. China. Electronic address: renlz@jlu.edu.cn.
authors.forename: Linzhu
authors.initials: L
authors.lastname: Ren
mesh_terms: D000818:Animals; D018173:Circoviridae Infections / Q000201:enzymology / Q000235:genetics / Q000662:veterinary* / Q000821:virology; D017925:Circovirus / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D005786:Gene Expression Regulation; D054884:Host-Pathogen Interactions; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent / Q000235:genetics* / Q000378:metabolism; D048055:Mitogen-Activated Protein Kinase 8 / Q000235:genetics / Q000378:metabolism; D048056:Mitogen-Activated Protein Kinase 9 / Q000235:genetics / Q000378:metabolism; D010766:Phosphorylation; D011493:Protein Kinase C / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D047428:Protein Kinase Inhibitors / Q000494:pharmacology; D015398:Signal Transduction; D013552:Swine; D013553:Swine Diseases / Q000201:enzymology / Q000235:genetics / Q000821:virology*; D014779:Virus Replication / Q000187:drug effects / Q000235:genetics
chemical_list: D047428:Protein Kinase Inhibitors; D025782:Hydroxymethylglutaryl-CoA-Reductases, NADP-dependent; D048056:Mitogen-Activated Protein Kinase 9; D011493:Protein Kinase C; D048055:Mitogen-Activated Protein Kinase 8
keywords: 3-hydroxy-3-methylglutaryl coenzyme a reductase (HMGCR); Porcine circovirus 2 (PCV2); Protein kinase C (PKC)