title: Island Cotton Enhanced Disease Susceptibility 1 Gene Encoding a Lipase-Like Protein Plays a Crucial Role in Response toVerticillium dahliaeby Regulating the SA Level and H2O2Accumulation.
abstract: Cotton is one of the most economically important crops, but most cultivated varieties lack adequate innate immunity or resistance toVerticilliumwilt. This results in serious losses to both yield and fiber quality. To identify the genetic resources for innate immunity and understand the pathways for pathogen defenses in this crop, here we focus on orthologs of the centralArabidopsis thalianadefense regulatorEnhanced Disease Susceptibility1 (EDS1). The full-length cDNA ofGbEDS1was obtained by screening the full-length cDNA library ofGossypium barbadensecombining with RACE strategy. Its open reading frame is 1848 bp long, encoding 615 amino acid residues. Sequence analysis showed that GbEDS1 contains a conserved N-terminal lipase domain and an EDS1-specific KNEDT motif. Expression profiling indicated that the gene is induced byVerticillium dahliaeas well as salicylic acid (SA) treatment. Subcellular localization assays revealed that GbEDS1 is located in the cell cytoplasm and nucleus. Overexpression ofGbEDS1inArabidopsisdramatically up-regulated SA and H2O2production, resulting in enhanced disease resistance toV. dahliae. Silencing ofGbEDS1inG. barbadensesignificantly decreased SA and H2O2accumulation, leading to the cotton more susceptibility. Moreover, combining the gene expression results from transgenicArabidopsisand silenced-GbEDS1 cotton, it indicated thatGbEDS1could activateGbNDR1andGbBAK1expression. These findings not only broaden our knowledge about the biological role ofGbEDS1, but also provide new insights into the defense mechanisms ofGbEDS1againstV. dahliaein cotton.
authors.affiliation: Department of Agronomy, North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University Baoding, China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Yan
authors.affiliation: Department of Agronomy, North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University Baoding, China.
authors.forename: Wang
authors.initials: W
authors.lastname: Xingfen
authors.affiliation: Department of Agronomy, North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University Baoding, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Wei
authors.affiliation: Department of Agronomy, North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University Baoding, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jun
authors.affiliation: Department of Agronomy, North China Key Laboratory for Germplasm Resources of Education Ministry, Hebei Agricultural University Baoding, China.
authors.forename: Ma
authors.initials: M
authors.lastname: Zhiying
keywords: EDS1; H2O2 accumulation; SA level; Verticillium wilt resistant; cotton (Gossypium barbadense); transgenic Arabidopsis; virus-induced gene silence