title: Cavin-2 Functions as a Suppressive Regulator in TNF-induced Mesenchymal Stromal Cell Inflammation and Angiogenic Phenotypes.
abstract: Tumour necrosis factor (TNF)-αactivation of mesenchymal stromal cells (MSC) enhances their tumour-suppressive properties and tumour-homing ability. The molecular actors involved are unknown. We found that TNF induced MSC migration and tubulogenesis which correlated with a dose-dependent increase inCavin-1 andCavin-3 transcript levels. TNF triggered cyclooxygenase (COX)-2 expression, whereas specific siRNA-mediated gene silencing ofCavin-2 resulted in an amplified COX-2 expression, tubulogenesis, and migratory response partially due to a rapid and sustained increase in NF-κB phosphorylation status. Our results highlight a suppressive role for the caveolar componentCavin-2 in the angiogenic and inflammatory regulation of TNF-activated MSC.
authors.affiliation: Laboratoire d'Oncologie Moléculaire, Département de Chimie, Centre de recherche BIOMED, Université du Québec à Montréal, Quebec, Canada.|Département de Physiologie Moléculaire et Intégrative, Faculté de Médecine, Université de Montréal, Montreal, Canada.
authors.forename: Bayader
authors.initials: B
authors.lastname: Annabi
authors.affiliation: Laboratoire d'Oncologie Moléculaire, Département de Chimie, Centre de recherche BIOMED, Université du Québec à Montréal, Quebec, Canada.
authors.forename: Alain
authors.initials: A
authors.lastname: Zgheib
authors.affiliation: Laboratoire d'Oncologie Moléculaire, Département de Chimie, Centre de recherche BIOMED, Université du Québec à Montréal, Quebec, Canada.
authors.forename: Borhane
authors.initials: B
authors.lastname: Annabi
keywords: Angiogenesis; Cancer; Cavin; Inflammation; Mesenchymal stromal cells; TNF