title: Variation of lifespan in multiple strains, and effects of dietary restriction and BmFoxO on lifespan in silkworm, Bombyx mori.
abstract: Established animal models have accelerated our understanding of the mechanisms involved in lifespan determination. However, more experimental animals are required to clarify the complex mechanisms behind the phenomena of aging and lifespan. In this study, we reported the variation of lifespan in nine distinct silkworm strains. Lifespan correlated significantly with BmFoxO gene expression in the representative silkworm strains tested (Xiafang, Dazao-N, and N4). In general, the female silkworm was longer lived than the male of the same strain. Dietary restriction extended the silkworm lifespan compared with that of silkworms fed ad libitum. The expression of BmFoxO was significantly elevated in the dietary restriction group on day 3 of the 4th instar and day 3 of the 5th instar, suggesting that BmFoxO contributes to dietary restriction-mediated lifespan extension. The RNA interference and overexpression of the BmFoxO gene significantly shortened and extended the silkworm adulthood, respectively. In conclusion, our findings suggest that the silkworm might serve as a promising experimental animal to explore the complex biological mechanisms of lifespan determination.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Jiangbo
authors.initials: J
authors.lastname: Song
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Dongmei
authors.initials: D
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Zhiquan
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Tong
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Jianfei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Minjin
authors.initials: M
authors.lastname: Han
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, College of Biotechnology, Southwest University, Chongqing, China.|Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, College of Biotechnology, Southwest University, Chongqing, China.
authors.forename: Fangyin
authors.initials: F
authors.lastname: Dai
mesh_terms: D000367:Age Factors; D000818:Animals; D012831:Bombyx / Q000235:genetics* / Q000254:growth & development / Q000378:metabolism; D031204:Caloric Restriction; D005260:Female; D051858:Forkhead Transcription Factors / Q000235:genetics* / Q000378:metabolism; D018507:Gene Expression Regulation, Developmental; D005838:Genotype; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D008136:Longevity / Q000235:genetics*; D008297:Male; D010641:Phenotype; D034622:RNA Interference; D012737:Sex Factors; D013997:Time Factors
chemical_list: D051858:Forkhead Transcription Factors; D019476:Insect Proteins
keywords: BmFoxO; Gerotarget; dietary restriction; experimental animal; lifespan; silkworm