title: Splicing features in the expression of the complementary-sense genes of Beet curly top Iran virus.
abstract: Beet curly top Iran virus (BCTIV) is a distinct geminivirus which has been reported from sugar-beet-growing farms in Iran. In this study, the role of the splicing in expression of complementary-sense genes of BCTIV was studied. Total RNA was extracted from BCTIV-infected tissue, and the predicted intron position of complementary-sense mRNA transcripts was amplified by RT-PCR followed by cloning of the amplicons. Sequence confirmed that both spliced and unspliced mRNAs are synthesized by the same transcription unit. Sequence comparison showed that a 155-nt segment (intron) corresponding to nucleotides 1890-2044 of the viral genome has been removed from the latter transcript and therefore fusion of the C1:C2 genes resulted creation of a continuous reading frame for potential production of intact replication initiator protein (Rep). BCTIV intron comprises of most consensus splicing signals required for splicing in eukaryotes and several plant viruses including mastre- and capulaviruses.
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Naghmeh
authors.initials: N
authors.lastname: Bozorgi
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran. jheydarnejad@uk.ac.ir.
authors.forename: Jahangir
authors.initials: J
authors.lastname: Heydarnejad
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Kamali
authors.affiliation: Department of Plant Protection, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
authors.forename: Hossain
authors.initials: H
authors.lastname: Massumi
mesh_terms: D027461:Beta vulgaris / Q000821:virology; D017791:Geminiviridae / Q000235:genetics* / Q000472:pathogenicity; D016679:Genome, Viral; D007492:Iran; D008969:Molecular Sequence Data; D010802:Phylogeny; D010935:Plant Diseases / Q000235:genetics / Q000821:virology; D012326:RNA Splicing / Q000235:genetics*; D014764:Viral Proteins / Q000235:genetics*; D014771:Virion / Q000235:genetics
chemical_list: D014764:Viral Proteins
keywords: Becurtovirus; Beet curly top Iran virus; Geminivirus; Intron; Splicing