title: Cytotoxic monoterpenoid indole alkaloids from Alstonia yunnanensis Diels.
abstract: The ethanol extract of the aerial parts of Alstonia yunnanensis Diels afforded five new monoterpenoid indole alkaloids, alstiyunnanenines A-E (1-5), along with one known compound, alstoniascholarine I (6). The structures of the isolated compounds were established based on 1D and 2D (1H-1H COSY, HMQC, and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated compounds were tested in vitro for cytotoxic potential using eight tumor cell lines. As a result, alkaloids 4-6 exhibited cytotoxicities against all tested tumor cell lines, especially against osteosarcoma cell lines (SOSP-9607, MG-63, Saos-2, M663) with IC50values<6μM.
authors.affiliation: Jinling Hospital, Department of Orthopedics, Nanjing University, School of Medicine, Zhongshan East Road 305, Nanjing, Jiangsu, 210002, Peoples R China.
authors.forename: Cheng-Jun
authors.initials: CJ
authors.lastname: Li
authors.affiliation: Jinling Hospital, Department of Orthopedics, Nanjing University, School of Medicine, Zhongshan East Road 305, Nanjing, Jiangsu, 210002, Peoples R China.
authors.forename: Shuo
authors.initials: S
authors.lastname: Chen
authors.affiliation: Jinling Hospital, Department of Orthopedics, Nanjing University, School of Medicine, Zhongshan East Road 305, Nanjing, Jiangsu, 210002, Peoples R China.
authors.forename: Chang
authors.initials: C
authors.lastname: Sun
authors.affiliation: Jinling Hospital, Department of Orthopedics, Nanjing University, School of Medicine, Zhongshan East Road 305, Nanjing, Jiangsu, 210002, Peoples R China.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Jinling Hospital, Department of Orthopedics, Nanjing University, School of Medicine, Zhongshan East Road 305, Nanjing, Jiangsu, 210002, Peoples R China.. Electronic address: xinshi201611@126.com.
authors.forename: Xin
authors.initials: X
authors.lastname: Shi
authors.affiliation: Jinling Hospital, Department of Orthopedics, Nanjing University, School of Medicine, Zhongshan East Road 305, Nanjing, Jiangsu, 210002, Peoples R China.. Electronic address: sujiawu2016@yeah.net.
authors.forename: Su-Jia
authors.initials: SJ
authors.lastname: Wu
mesh_terms: D031051:Alstonia / Q000737:chemistry*; D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification; D045744:Cell Line, Tumor; D006801:Humans; D015394:Molecular Structure; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000737:chemistry; D046948:Secologanin Tryptamine Alkaloids / Q000737:chemistry* / Q000302:isolation & purification
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D010936:Plant Extracts; D046948:Secologanin Tryptamine Alkaloids
keywords: Alstonia yunnanensis Diels; Apocynaceae; Cytotoxicity; Monoterpenoid indole alkaloids