title: Novel impedimetric dopamine biosensor based on boronic acid functional polythiophene modified electrodes.
abstract: In this study we report a new, simple and first impedimetric biosensor based on 3-Thienyl boronic acid for dopamine detection. Biosensor electrode preparation is 1min long by simple electro-polymerization of 3-Thienyl boronic acid and copolymer Thiophene P(TBA0.50Th0.50). Strong interaction between dopamine and thin layer of boronic acid has provided bio-sensing electrode high selectivity and stability, linear range of 7.8 to 125μM, and detection limit of 0.3μM. Characterization and optimization studies were conducted using electrochemical impedance spectroscopy (EIS) and cyclic voltammogram (CV). In order to test reliability of proposed biosensor real sample application study has been conducted using non-diluted human urine and it has been found that biosensor selectivity and recovery is excellent. As well P(TBA0.50Th0.50) based electrode and dopamine interaction has been proven by single frequency impedance measurements. Biosensors acquired good reproducibility, stability, selectivity and very low interference.
authors.affiliation: Institute of Biomedical Engineering, Fatih University, B. Cekmece, Istanbul 34500, Turkey.
authors.forename: Muamer
authors.initials: M
authors.lastname: Dervisevic
authors.affiliation: Institute of Biomedical Engineering, Fatih University, B. Cekmece, Istanbul 34500, Turkey. Electronic address: msenel81@gmail.com.
authors.forename: Mehmet
authors.initials: M
authors.lastname: Senel
authors.affiliation: Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B. Cekmece, Istanbul 34500, Turkey.
authors.forename: Emre
authors.initials: E
authors.lastname: Cevik
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D058266:Dielectric Spectroscopy; D004298:Dopamine / Q000032:analysis* / Q000652:urine; D004566:Electrodes; D006801:Humans; D057230:Limit of Detection; D008855:Microscopy, Electron, Scanning; D011108:Polymers / Q000737:chemistry*; D013876:Thiophenes / Q000737:chemistry*
chemical_list: D011108:Polymers; D013876:Thiophenes; C066730:polythiophene; D004298:Dopamine
keywords: Boronic acid; Dopamine; Electrochemical impedance spectroscopy; Thiophene