title: Carbon coated magnetic nanoparticles as a novel magnetic solid phase extraction adsorbent for simultaneous extraction of methamphetamine and ephedrine from urine samples.
abstract: This paper develops a highly selective, specific and efficient method for simultaneous determination of ephedrine and methamphetamine by a new carbon coated magnetic nanoparticles (C/MNPs) as a magnetic solid phase extraction (MSPE) adsorbent in biological urine medium. The characterization of synthesized magnetic nano adsorbent was completely carried out by various characterization techniques like Fourier transform infrared (FT-IR) spectroscopy, powder x-ray diffraction (XRD), scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM). Nine important parameters influencing extraction efficiency including amount of adsorbent, amounts of sample volume, pH, type and amount of extraction organic solvent, time of extraction and desorption, agitation rate and ionic strength of extraction medium, were studied and optimized. Under optimized extraction conditions, a good linearity was observed in the concentration range of 100-2000ng/mL for ephedrine and 100-2500ng/mL for methamphetamine. Analysis of positive urine samples was carried out by proposed method with the recovery of 98.71 and 97.87% for ephedrine and methamphetamine, respectively. The results indicated that carbon coated magnetic nanoparticles could be applied in clinical and forensic laboratories for simultaneous determination of abused drugs in urine media.
authors.affiliation: Biotechnology Research Centre, Tabriz University of Medical Science, Tabriz, Iran.
authors.forename: Arezou
authors.initials: A
authors.lastname: Taghvimi
authors.affiliation: Drug Applied Research Centre, Tabriz University of Medical Science, Tabriz, Iran. Electronic address: Hamishehkarh@tbzmed.ac.ir.
authors.forename: Hamed
authors.initials: H
authors.lastname: Hamishehkar
mesh_terms: D002244:Carbon / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D004809:Ephedrine / Q000302:isolation & purification / Q000652:urine*; D006801:Humans; D057230:Limit of Detection; D016014:Linear Models; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D008694:Methamphetamine / Q000302:isolation & purification / Q000652:urine*; D015203:Reproducibility of Results; D052616:Solid Phase Extraction / Q000379:methods*
chemical_list: D058185:Magnetite Nanoparticles; D008694:Methamphetamine; D002244:Carbon; D004809:Ephedrine
keywords: Carbon coated magnetic nanoparticles; Ephedrine; HPLC; Magnetic solid phase extraction; Methamphetamine; Urine