title: Evaluation of anti-cholinesterase, antibacterial and cytotoxic activities of green synthesized silver nanoparticles using from Millettia pinnata flower extract.
abstract: The aim of this study is to develop an easy and eco-friendly method for the synthesis of Ag-NPs using extracts from the medicinal plant, Millettia pinnata flower extract and investigate the effects of Ag-NPs on acetylcholinesterase (AChE), butyrylcholinesterase (BChE), antibacterial and cytotoxicity activity. UV-Vis peak at 438 nm confirmed the Ag-NPs absorbance. The SEM analysis results confirmed the presence of spherical shaped Ag-NPs by a huge disparity in the particle size distribution with an average size of 49 ± 0.9 nm. TEM images revealed the formation of Ag-NPs with spherical shape and sizes in the range between 16 and 38 nm. The Ag-NPs showed an excellent inhibitory efficacy against AChE and BChE. The highest antibacterial activity was found against Escherichia coli (20.25 ± 0.91 mm). These nanoparticles showed the cytotoxic effects against brine shrimp (artemia saliana) nauplii with a LD50value of 33.92.
authors.affiliation: Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 143 701, South Korea.
authors.forename: Govindasamy
authors.initials: G
authors.lastname: Rajakumar
authors.affiliation: Department of Chemistry, D.K.M. College for Women, Vellore, Tamil Nadu, India. Electronic address: drgoms1@gmail.com.
authors.forename: Thandapani
authors.initials: T
authors.lastname: Gomathi
authors.affiliation: Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 143 701, South Korea.
authors.forename: Muthu
authors.initials: M
authors.lastname: Thiruvengadam
authors.affiliation: Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore -14, Tamil Nadu, India.
authors.forename: V
authors.initials: V
authors.lastname: Devi Rajeswari
authors.affiliation: Department of Biomedical Sciences, School of Biosciences and Technology, VIT University, Vellore -14, Tamil Nadu, India.
authors.forename: V N
authors.initials: VN
authors.lastname: Kalpana
authors.affiliation: Department of Applied Bioscience, College of Life and Environmental Science, Konkuk University, Seoul 143 701, South Korea. Electronic address: imchung@konkuk.ac.kr.
authors.forename: Ill-Min
authors.initials: IM
authors.lastname: Chung
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology*; D004789:Enzyme Activation / Q000187:drug effects; D035264:Flowers / Q000737:chemistry*; D055772:Green Chemistry Technology; D053768:Metal Nanoparticles / Q000008:administration & dosage / Q000737:chemistry / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D033641:Millettia / Q000737:chemistry*; D056951:Photoelectron Spectroscopy; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D012834:Silver / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D002800:Cholinesterase Inhibitors; D010936:Plant Extracts; D012834:Silver
keywords: Anti-cholinesterase; Antibacteria; Electron microscopy; Millettia pinnata; Silver nanoparticles