title: Evaluation of oxidative stress induction in rats following exposure to silver nanorods.
abstract: The study investigated the oxidative stress induction by the 10 and 25 nm silver nanorods (SNRs) following intra-tracheal instillation in rats after 1 day, 1 week, 1 month and 3 months post instillation periods at 1 and 5 mg/kg b.w. doses. The blood was withdrawn by retro orbital plexus method after exposure periods and different oxidative stress markers were estimated. The results showed that the both sizes of SNRs induced increased levels of malondialdehyde (MDA) and depleted glutathione (GSH) levels after 1 day and 1 week post exposure periods. The 10 and 25 nm SNRs at both doses displayed that significantly reduced levels of superoxide dismutase (SOD) and catalase following 1 day and 1 week post exposure periods. Also, the results have shown that decrease in total antioxidant capacity (TAC) of both sizes of SNRs significantly following 1 day and 1 week post exposure periods, indicating the oxidative stress induction by SNRs. In spite, there were no significant changes in oxidative stress markers following 1 month and 3 months post exposure periods may be due to recovery. The increased levels of MDA and decreased levels of GSH, SOD, catalase and TAC activity are strongly associated to ROS production and lipid peroxidation, suggesting the induction of oxidative stress in rats. The 10 nm SNRs at 5 mg/kg b.w. dose exposures in rats have shown greater changes in all oxidative stress parameters, indicating the greater induction of oxidative stress when compared with the 25 nm SNRs, representing the size-dose-dependent induction of oxidative stress of SNRs.
authors.affiliation: a Department of Pharmacology and Toxicology , University College of Pharmaceutical Sciences, Kakatiya University , Warangal , Telangana , India.
authors.forename: Harikiran
authors.initials: H
authors.lastname: Lingabathula
authors.affiliation: a Department of Pharmacology and Toxicology , University College of Pharmaceutical Sciences, Kakatiya University , Warangal , Telangana , India.
authors.forename: Narsimhareddy
authors.initials: N
authors.lastname: Yellu
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D007322:Instillation, Drug; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D043942:Nanotubes / Q000737:chemistry / Q000633:toxicity*; D018384:Oxidative Stress / Q000187:drug effects*; D010316:Particle Size; D011791:Quartz / Q000737:chemistry / Q000633:toxicity; D017208:Rats, Wistar; D012834:Silver / Q000737:chemistry / Q000633:toxicity*
chemical_list: D000975:Antioxidants; D015415:Biomarkers; D011791:Quartz; D012834:Silver; D008315:Malondialdehyde
keywords: Lipid peroxidation; oxidative stress; silver nanorods; superoxide dismutase; toxicity