title: Allogeneic Mesenchymal Stem Cell Transplantation in Dogs With Keratoconjunctivitis Sicca.
abstract: Keratoconjunctivitis sicca (KCS) is a dysfunction in tear production associated with clinical signs, which include conjunctival hyperemia, ocular discharge, discomfort, pain, and, eventually, corneal vascularization and pigmentation. Immunosuppressive drugs are routinely administrated for long periods to treat KCS but with side effects and limited results. Evaluation of the clinical benefits of intralacrimal transplantation of allogeneic mesenchymal stem cells (MSCs) in dogs with mild-moderate and severe KCS was done. A total of 24 eyes with KCS from 15 dogs of different breeds were enrolled in the present study. A single transplantation of MSCs (1 × 106) directly into lacrimal glands (dorsal and third eyelid) was performed. The Schirmer tear tests (STTs) and ocular surface improvements were used to assess short- and long-term effects of these cells. The STTs were carried out on day 0 (before MSCs transplantation) and on days 7, 14, 21, and 28, as well as 6 and 12 months after MSC transplantation. Our data demonstrate that allogeneic MSC transplantation in KCS dogs is safe since no adverse effects were observed immediately after transplantation and in short- and long-term follow-ups. A statistically significant increase in the STT and ocular surface improvements was found in all eyes studied. In all the eyes with mild-moderate KCS, STT values reverted to those of healthy eyes, while in eyes with severe KCS, although complete reversion was not found, there was improvement in tear production and in other clinical signs. Our study shows that a single dose of a low number of MSCs can be used to treat KCS in dogs. In contrast to immunosuppressive drug use, MSC transplantation has an effect over a long period (up to 12 months), even after a single administration, and does not require daily drug administration.
authors.affiliation: Department of Ophthalmology, Universidade Estadual de Campinas (UNICAMP) , Campinas, SP , Brazil.
authors.forename: Maura K W
authors.initials: MK
authors.lastname: Bittencourt
authors.affiliation: † Regenera Medicina Veterinária Avançada , Campinas, SP , Brazil.
authors.forename: Michele A
authors.initials: MA
authors.lastname: Barros
authors.affiliation: † Regenera Medicina Veterinária Avançada , Campinas, SP , Brazil.
authors.forename: João Flávio P
authors.initials: JF
authors.lastname: Martins
authors.affiliation: Department of Ophthalmology, Universidade Estadual de Campinas (UNICAMP) , Campinas, SP , Brazil.
authors.forename: Jose Paulo C
authors.initials: JP
authors.lastname: Vasconcellos
authors.affiliation: † Regenera Medicina Veterinária Avançada , Campinas, SP , Brazil.
authors.forename: Bruna P
authors.initials: BP
authors.lastname: Morais
authors.affiliation: ‡ Instituto Butantan , São Paulo, SP , Brazil.
authors.forename: Celine
authors.initials: C
authors.lastname: Pompeia
authors.affiliation: § Private Practice Ophthalmologist, Benvista Oftalmologia, Indaiatuba , São Paulo, SP , Brazil.
authors.forename: Matheus Domingues
authors.initials: MD
authors.lastname: Bittencourt
authors.affiliation: ¶ Universidad Nacional de Rio Cuarto , Cordoba , Argentina.
authors.forename: Karine Dos Santos
authors.initials: KD
authors.lastname: Evangelho
authors.affiliation: ‡ Instituto Butantan , São Paulo, SP , Brazil.
authors.forename: Irina
authors.initials: I
authors.lastname: Kerkis
authors.affiliation: ‡ Instituto Butantan , São Paulo, SP , Brazil.
authors.forename: Cristiane V
authors.initials: CV
authors.lastname: Wenceslau
keywords: Allogeneic mesenchymal stem cell transplantation; Dogs; Dry eye syndrome; Keratoconjunctivitis sicca (KCS); Schirmer tear test (STT)