title: Effects of resveratrol on ARPE-19 cell proliferation and migrationviaregulating the expression of proliferating cell nuclear antigen, P21, P27 and p38MAPK/MMP-9.
abstract: OBJECTIVE
  To explore whether resveratrol (Res) can inhibit human retinal pigment epithelial cell (ARPE-19 cell) proliferation and migration, and to research the molecular mechanisms.
  
  
  METHODS
  ARPE-19 cells were pretreated with various concentrations at 0, 50, 100, 150, 200 and 300 µmol/L of Res, and with 0 µmol/L Res as the control for 24, 48 and 72h. The cell proliferation, apoptosis and migration were measured with cell counting kit-8 (CCK-8), flow cytometry, and wound-healing and Transwell assays, respectively. The expression of proliferating cell nuclear antigen (PCNA), P21 and P27, as well as matrix metalloproteinase-9 (MMP-9) and p38 mitogen-activated protein kinases (p38MAPK) was identified by Western blot.
  
  
  RESULTS
  Cell proliferation was effectively inhibited by Res (P<0.05). When pretreated with Res, cells arrested in S-phase increased remarkably (P<0.05), but the apoptosis ratios showed no significant difference between the treatment and control groups (P>0.05). Cell migration was suppressed by Res both in wound-healing assay and Transwell migration assay (P<0.05). Decreases of PCNA, MMP-9 and p38MAPK, as well as increases of P21 and P27 were detected by Western blot (P<0.05).
  
  
  CONCLUSIONS
  Res can inhibit APRE-19 cell proliferation and migration in a concentration-dependent manner with up-regulation of the expression of P21 and P27, and down-regulation of PCNA, MMP-9 and p38MAPK.
authors.affiliation: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Xiao-Ning
authors.initials: XN
authors.lastname: Hao
authors.affiliation: Department of Ophthalmology, Shenzhen Children's Hospital, Shenzhen 518026, Guangdong Province, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Wang
authors.affiliation: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Zhou
authors.affiliation: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Yi-Xin
authors.initials: YX
authors.lastname: Qu
authors.affiliation: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Xiao-Yong
authors.initials: XY
authors.lastname: Liu
authors.affiliation: Department of Ophthalmology, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Xu
keywords: cell migration inhibition; cell proliferation; resveratrol; retinal pigment epithelium