title: Genetic Polymorphisms of SP-A, SP-B, and SP-D and Risk of Respiratory Distress Syndrome in Preterm Neonates.
abstract: BACKGROUND We examined selected polymorphisms in 3 pulmonary surfactant-associated proteins (SP) for their influence on serum SP levels and risk of respiratory distress syndrome (RDS) in preterm neonates. MATERIAL AND METHODS Premature infants from a Han population were enrolled, including 100 premature infants with RDS (case group) and 120 premature infants without RDS (control group). SNP genotyping for SP-A (+186A/G and +655C/T), SP-B (-18A/C and 1580C/T), and SP-D (Met11ThrT/C and Ala160ThrG/A) used polymerase chain reaction-restriction fragment length polymorphism. Haplotypes were calculated with Shesis software and serum SP-A/B/D levels were quantified by ELISA. RESULTS Case and control groups exhibited significant differences in genotype and allele frequencies of SP-A (+186A/G, +655C/T) and SP-B (1580C/T). However, no statistically significant differences were observed in the allele and genotype frequencies of SP-B -18A/C, SP-D Met11ThrT/C, and SP-D Ala160ThrG/A. Importantly, serum SP-A and SP-B levels were reduced in RDS patients carrying SP-A (+186A/G, +655C/T) and SP-B (1580C/T) polymorphisms. AA genotype of +186A/G, SP-A level, and CC genotype of 1580C/T were independently correlated with increased RDS risk. CONCLUSIONS SP-A (+186A/G) and SP-B (1580C/T) polymorphisms are strongly associated with the risk of RDS in preterm infants. Notably, reduced serum SP-A levels were correlated with a high risk of RDS and may serve as novel biomarkers for RDS detection and monitoring.
authors.affiliation: Department of Pediatrics, The General Hospital of the PLA Rocket Force, Beijing, China (mainland).
authors.forename: Hong-Yu
authors.initials: HY
authors.lastname: Chang
authors.affiliation: Department of Pediatrics, The General Hospital of the PLA Rocket Force, Beijing, China (mainland).
authors.forename: Fang
authors.initials: F
authors.lastname: Li
authors.affiliation: Central Laboratory, The General Hospital of the PLA Rocket Force, Beijing, China (mainland).
authors.forename: Feng-Sheng
authors.initials: FS
authors.lastname: Li
authors.affiliation: Department of Pediatrics, The 306 Hospital of PLA, Beijing, China (mainland).
authors.forename: Cheng-Zhong
authors.initials: CZ
authors.lastname: Zheng
authors.affiliation: Department of Neonatology, Haidian Maternal and Child Healthcare Hospital, Beijing, China (mainland).
authors.forename: Yan-Zhe
authors.initials: YZ
authors.lastname: Lei
authors.affiliation: Department of Neonatology, Nanjing Tongren Hospital, Nanjing, China (mainland).
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
mesh_terms: D016022:Case-Control Studies; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D020022:Genetic Predisposition to Disease; D006239:Haplotypes / Q000235:genetics; D006801:Humans; D007231:Infant, Newborn; D007234:Infant, Premature / Q000378:metabolism*; D016015:Logistic Models; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D037662:Pulmonary Surfactant-Associated Protein A / Q000097:blood / Q000235:genetics*; D037701:Pulmonary Surfactant-Associated Protein B / Q000097:blood / Q000235:genetics*; D037663:Pulmonary Surfactant-Associated Protein D / Q000097:blood / Q000235:genetics*; D012127:Respiratory Distress Syndrome, Newborn / Q000097:blood / Q000235:genetics*; D012307:Risk Factors
chemical_list: D037662:Pulmonary Surfactant-Associated Protein A; D037701:Pulmonary Surfactant-Associated Protein B; D037663:Pulmonary Surfactant-Associated Protein D