title: How charge distribution influences the function of membrane-active peptides: Lytic or cell-penetrating?
abstract: Lytic and cell-penetrating peptides (CPPs) are both membrane-active peptides sharing similar physicochemical properties. Although their respective functions have been intensively investigated, the difference of intrinsic properties between these two types of peptides is rarely discussed. In this study, we designed a series of analogs of a recently discovered CPP ZXR-1 (FKIGGFIKKLWRSKLA) by varying the charge distributions both on the helical wheel projection and along the sequence. These peptides showed different functions on cell membranes, including membrane lytic (peptide Z1), cell-penetrating (peptide ZXR-1, Z2 and Z3), and inactive (peptide Z4) peptides. The three groups of peptides displayed different interactions with model lipid monolayer, and found that peptide insertion might be an important dynamic step to distinguish lytic and cell penetrating functions. Based on the analysis of charge distribution patterns, it was proposed that the charge distributions on the helical wheel and along the sequence are both able to influence the functions of the membrane-active peptides. This finding provides a further understanding about the effect of charge distribution on the functions of membrane-active peptides, and will be helpful for the design of functional peptides.
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Long
authors.initials: L
authors.lastname: Chen
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Xiushuang
authors.initials: X
authors.lastname: Yuan
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yimeng
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Yuan
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Huiwei
authors.initials: H
authors.lastname: Yin
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China.
authors.forename: Xiufang
authors.initials: X
authors.lastname: Ding
authors.affiliation: State Key Laboratory of NBC Protection for Civilian, Beijing 102205, China.
authors.forename: Zhentai
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Beijing Key Laboratory of Bioprocess, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China. Electronic address: luosz@mail.buct.edu.cn.
authors.forename: Shi-Zhong
authors.initials: SZ
authors.lastname: Luo
mesh_terms: D000595:Amino Acid Sequence; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D015195:Drug Design; D006367:HeLa Cells; D006801:Humans; D008563:Membrane Lipids / Q000378:metabolism; D010455:Peptides / Q000737:chemistry* / Q000378:metabolism* / Q000633:toxicity; D017433:Protein Structure, Secondary; D021381:Protein Transport; D055672:Static Electricity
chemical_list: D057846:Cell-Penetrating Peptides; D008563:Membrane Lipids; D010455:Peptides; C000624237:ZXR-1 peptide
keywords: Cell penetrating peptides; Charge distribution; Lytic peptides; Peptide design; Peptide-membrane interactions