title: Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity.
abstract: Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity.
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Priscilla Stela Santana
authors.initials: PS
authors.lastname: de Oliveira
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Michelly Cristiny
authors.initials: MC
authors.lastname: Pereira
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Simão Kalebe
authors.initials: SK
authors.lastname: Silva de Paula
authors.affiliation: Hospital das Clínicas, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Emerson Vasconcelos Andrade
authors.initials: EV
authors.lastname: Lima
authors.affiliation: Hospital das Clínicas, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Mariana Modesto de Andrade
authors.initials: MM
authors.lastname: Lima
authors.affiliation: Faculdade Nova Roma, Recife, PE, Brazil.
authors.forename: Rodrigo Gomes
authors.initials: RG
authors.lastname: de Arruda
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Wagner Luís Mendes
authors.identifier: 0000-0001-8555-4093
authors.initials: WL
authors.lastname: de Oliveira
authors.affiliation: Hospital das Clínicas, Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Ângela Luzia Branco Pinto
authors.initials: ÂL
authors.lastname: Duarte
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Ivan da Rocha
authors.initials: ID
authors.lastname: Pitta
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Moacyr Jesus Melo Barreto
authors.identifier: 0000-0002-1883-6012
authors.initials: MJ
authors.lastname: Rêgo
authors.affiliation: Laboratório de Imunomodulação e Novas Abordagens Terapêuticas (LINAT), Núcleo de Pesquisa em Inovação Terapêutica Suely Galdino (NUPIT-SG), Universidade Federal de Pernambuco (UFPE), Recife, PE, Brazil.
authors.forename: Maira
authors.identifier: 0000-0001-5881-0338
authors.initials: M
authors.lastname: Galdino da Rocha Pitta
mesh_terms: D000328:Adult; D000368:Aged; D001938:Brazil; D015331:Cohort Studies; D015897:Comorbidity; D016207:Cytokines / Q000378:metabolism; D003880:Dermatology; D005260:Female; D051858:Forkhead Transcription Factors / Q000378:metabolism*; D006801:Humans; D007371:Interferon-gamma / Q000378:metabolism*; D020381:Interleukin-17 / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011565:Psoriasis / Q000188:drug therapy / Q000378:metabolism*; D012867:Skin / Q000378:metabolism / Q000473:pathology; D018417:Th1 Cells / Q000378:metabolism; D058504:Th17 Cells / Q000378:metabolism; D055815:Young Adult
chemical_list: D016207:Cytokines; C418974:FOXP3 protein, human; D051858:Forkhead Transcription Factors; C573415:IFNG protein, human; C508717:IL17A protein, human; D020381:Interleukin-17; D007371:Interferon-gamma