title: Examination of in vivo mutagenicity of sodium arsenite and dimethylarsinic acid in gpt delta rats.
abstract: Arsenic is a well-known human bladder and liver carcinogen, but its exact mechanism of carcinogenicity is not fully understood. Dimethylarsinic acid (DMAV) is a major urinary metabolite of sodium arsenite (iAsIII) and induces urinary bladder cancers in rats. DMAVand iAsIIIare negative in in vitro mutagenicity tests. However, their in vivo mutagenicities have not been determined. The purpose of present study is to evaluate the in vivo mutagenicities of DMAVand iAsIIIin rat urinary bladder epithelium and liver using gpt delta F344 rats. Ten-week old male gpt delta F344 rats were randomized into 3 groups and administered 0, 92mg/L DMAV, or 87mg/L iAsIII(each 50mg/L As) for 13weeks in the drinking water. In the mutation assay, point mutations are detected in the gpt gene by 6-thioguanine selection (gpt assay) and deletion mutations are identified in the red/gam genes by Spi-selection (Spi-assay). Results of the gpt and Spi-assays showed that DMAVand iAsIIIhad no effects on the mutant frequencies or mutation spectrum in urinary bladder epithelium or liver. These findings indicate that DMAVand iAsIIIare not mutagenic in urinary bladder epithelium or liver in rats.
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan. Electronic address: mtajiri@med.osaka-cu.ac.jp.
authors.forename: Masaki
authors.initials: M
authors.lastname: Fujioka
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan.
authors.forename: Min
authors.initials: M
authors.lastname: Gi
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan.
authors.forename: Satoko
authors.initials: S
authors.lastname: Kawachi
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan.
authors.forename: Kumiko
authors.initials: K
authors.lastname: Tatsumi
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan.
authors.forename: Naomi
authors.initials: N
authors.lastname: Ishii
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan.
authors.forename: Kenichiro
authors.initials: K
authors.lastname: Doi
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan.
authors.forename: Anna
authors.initials: A
authors.lastname: Kakehashi
authors.affiliation: Department of Molecular Pathology, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-machi, Abeno-ku, 545-8585, Osaka, Japan. Electronic address: wani@med.osaka-cu.ac.jp.
authors.forename: Hideki
authors.initials: H
authors.lastname: Wanibuchi
mesh_terms: D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D002101:Cacodylic Acid / Q000633:toxicity*; D002273:Carcinogens / Q000633:toxicity*; D029968:Escherichia coli Proteins / Q000235:genetics; D008099:Liver; D009152:Mutagenicity Tests; D010430:Pentosyltransferases / Q000235:genetics; D051381:Rats; D011916:Rats, Inbred F344; D017670:Sodium Compounds / Q000633:toxicity*; D019459:Urothelium
chemical_list: D018053:Arsenites; D002273:Carcinogens; D029968:Escherichia coli Proteins; D017670:Sodium Compounds; C017947:sodium arsenite; D002101:Cacodylic Acid; D010430:Pentosyltransferases; C071962:Gpt protein, E coli
keywords: Dimethylarsinic acid; In vivo mutagenicity; Sodium arsenite; Urinary bladder epithelium; gpt delta rat