title: Antidepressant like effects of hydrolysable tannins of Terminalia catappa leaf extract via modulation of hippocampal plasticity and regulation of monoamine neurotransmitters subjected to chronic mild stress (CMS).
abstract: Terminalia catappa L. belonging to Combretaceae family is a folk medicine, known for its multiple pharmacological properties, but the neuro-modulatory effect of TC against chronic mild stress was seldom explored. The present study was designed to elucidate potential antidepressant-like effect of Terminalia cattapa (leaf) hydro-alcoholic extract (TC) by using CMS model for a period of 7 weeks. Identification of hydrolysable tannins was done by using LC-MS. After the CMS exposure, mice groups were administered with imipramine (IMP, 10mg/kg, i.p.) and TC (25, 50 and 100mg/kg of TC, p.o.). Behavioural paradigms used for the study included forced swimming test (FST), tail suspension test (TST) and sucrose preference test (SPT). After behavioural tests, monoamine neurotransmitter, cortisol, AchE, oxidative stress levels and mRNA expression studies relevant to depression were assessed. TC supplementation significantly reversed CMS induced immobility time in FST and other behavioural paradigms. Moreover, TC administration significantly restored CMS induced changes in concentrations of hippocampal neurotransmitters (5-HT, DA and NE) as well as levels of acetyl cholinesterase, cortisol, monoamine oxidases (MAO-A, MAO-B), BDNF, CREB, and p-CREB. It suggests that TC supplementation could supress stress induced depression by regulating monoamine neurotransmitters, CREB, BDNF, cortisol, AchE level as well as by amelioration of oxidative stress. Hence TC can be used as a complementary medicine against depression-like disorder.
authors.affiliation: Applied Nutrition Division, Defence Food Research Laboratory, DRDO, Mysore 570011, Karnataka, India. Electronic address: sekhar268@gmail.com.
authors.forename: Y
authors.initials: Y
authors.lastname: Chandrasekhar
authors.affiliation: Applied Nutrition Division, Defence Food Research Laboratory, DRDO, Mysore 570011, Karnataka, India.
authors.forename: E M
authors.initials: EM
authors.lastname: Ramya
authors.affiliation: Applied Nutrition Division, Defence Food Research Laboratory, DRDO, Mysore 570011, Karnataka, India.
authors.forename: K
authors.initials: K
authors.lastname: Navya
authors.affiliation: Applied Nutrition Division, Defence Food Research Laboratory, DRDO, Mysore 570011, Karnataka, India. Electronic address: phani_bot@rediffmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Phani Kumar
authors.affiliation: Applied Nutrition Division, Defence Food Research Laboratory, DRDO, Mysore 570011, Karnataka, India. Electronic address: anilakumarkr@gmail.com.
authors.forename: K R
authors.initials: KR
authors.lastname: Anilakumar
mesh_terms: D000818:Animals; D000928:Antidepressive Agents / Q000494:pharmacology*; D001522:Behavior, Animal / Q000187:drug effects; D015306:Biogenic Monoamines / Q000378:metabolism*; D003863:Depression / Q000188:drug therapy / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D019417:Hindlimb Suspension / Q000523:psychology; D006624:Hippocampus / Q000187:drug effects* / Q000378:metabolism; D047348:Hydrolyzable Tannins / Q000494:pharmacology*; D051379:Mice; D008807:Mice, Inbred BALB C; D018377:Neurotransmitter Agents / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry; D013315:Stress, Psychological / Q000188:drug therapy* / Q000378:metabolism; D013550:Swimming / Q000523:psychology; D029766:Terminalia / Q000737:chemistry*
chemical_list: D000928:Antidepressive Agents; D015306:Biogenic Monoamines; D047348:Hydrolyzable Tannins; D018377:Neurotransmitter Agents; D010936:Plant Extracts
keywords: BDNF; Chronic mild stress; Hippocampus; Hydrolysable tannins; Terminalia catappa