title: Functional proteomic insights in B-cell chronic lymphocytic leukemia.
abstract: B cell chronic lymphocytic leukemia (B-CLL) is a hematological malignancy considered as the most common leukemia in the Western world. The understanding of B cell differentiation is crucial for the diagnosis, prognosis, and treatment of the disease. Areas covered: In this review, B-cell ontogeny and its relation with the CLL development, in combination with the proteomic approaches which could provide a deep characterization of the disease through the characterization of the cellular signaling pathways involved in the pathological cells is described. Expert commentary: Although conventional strategies (genome sequencing, morphology assays, and immunophenotyping by flow cytometry and/or immunochemistry) have allowed the establishment of the disease stage based on different parameters, it is still necessary to utilize novel approaches (e.g., proteomics) that have the potential to simultaneously analyze thousands of molecules to improve understanding of CLL.
authors.affiliation: a Department of Medicine and General Cytometry Service-Nucleus , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.|b Proteomics Unit , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: a Department of Medicine and General Cytometry Service-Nucleus , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Góngora
authors.affiliation: a Department of Medicine and General Cytometry Service-Nucleus , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.
authors.forename: Alberto
authors.initials: A
authors.lastname: Orfao
authors.affiliation: a Department of Medicine and General Cytometry Service-Nucleus , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.|b Proteomics Unit , Cancer Research Centre (IBMCC/CSIC/USAL/IBSAL) , Salamanca , Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Fuentes
mesh_terms: D006801:Humans; D015451:Leukemia, Lymphocytic, Chronic, B-Cell / Q000378:metabolism* / Q000473:pathology*; D040901:Proteomics / Q000379:methods*
keywords: CLL heterogeneity; Chronic lymphocytic leukemia; cytogenetics; proteomics; signaling pathways