title: Using Confocal Microscopy and Computational Modeling to Investigate the Cell-Penetrating Properties of Antimicrobial Peptides.
abstract: Antimicrobial peptides (AMPs) may display the ability to penetrate cells, which may be relevant for their antibiotic activity. To investigate the relevance of the penetrating activity for the antibiotic activity of AMPs, here we describe a method based on the combined use of confocal microscopy and computational modeling coupled with cell death kinetics.
authors.affiliation: Biochemistry and Structural Biology Department, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior S/N Ciudad Universitaria, México D.F., 04510, Mexico. gdelrio@ifc.unam.mx.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Del Rio
authors.affiliation: Theoretische und Molekulare Biophysik, Humboldt-Universität zu Berlin, Berlin, Germany.
authors.forename: Edda
authors.initials: E
authors.lastname: Klipp
authors.affiliation: Theoretische und Molekulare Biophysik, Humboldt-Universität zu Berlin, Berlin, Germany.
authors.forename: Andreas
authors.initials: A
authors.lastname: Herrmann
mesh_terms: D000465:Algorithms; D000890:Anti-Infective Agents / Q000737:chemistry* / Q000378:metabolism; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000737:chemistry* / Q000378:metabolism; D003198:Computer Simulation; D005456:Fluorescent Dyes; D018613:Microscopy, Confocal; D008962:Models, Theoretical; D013194:Staining and Labeling
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D057846:Cell-Penetrating Peptides; D005456:Fluorescent Dyes
keywords: Antimicrobial peptides; Cell death kinetics; Cell-penetrating peptides; Confocal microscopy; Mathematical modeling; Multifunctional peptides