title: Evaluation of Pyrrolidonyl Arylamidase Activity in Staphylococcus delphini.
abstract: Clinical reference textbooks lack data for pyrrolidonyl arylamidase (PYR) activity inStaphylococcus delphiniThis study evaluated PYR activities of 21S. delphinistrains by reference broth, rapid disc, and rapid slide methods. Species and subgroup identifications were confirmed by nucleic acid-based methods and included nine group A and 12 group B strains. Testing by rapid PYR methods with products from four manufacturers was performed at two testing locations, and, with the exception of one strain tested at one location using reagents from one manufacturer, eachS. delphinistrain tested positive for PYR activity. Therefore, PYR may be a useful single-test adjunct for distinguishingStaphylococcus aureusfromS. delphiniand other members of theStaphylococcus intermediusgroup.
authors.affiliation: Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
authors.forename: Samantha T
authors.initials: ST
authors.lastname: Compton
authors.affiliation: Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA.
authors.forename: Stephen A
authors.initials: SA
authors.lastname: Kania
authors.affiliation: NewYork-Presbyterian/Weill Cornell Medical Center, New York, New York, USA.
authors.forename: Amy E
authors.initials: AE
authors.lastname: Robertson
authors.affiliation: Department of Veterinary Pathobiology, Texas A&M University College of Veterinary Medicine, College Station, Texas, USA.
authors.forename: Sara D
authors.identifier: 0000-0001-9154-8909
authors.initials: SD
authors.lastname: Lawhon
authors.affiliation: Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA.
authors.forename: Stephen G
authors.initials: SG
authors.lastname: Jenkins
authors.affiliation: Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, New York, USA law9067@med.cornell.edu bemis@utk.edu.
authors.forename: Lars F
authors.initials: LF
authors.lastname: Westblade
authors.affiliation: Department of Biomedical and Diagnostic Sciences, University of Tennessee College of Veterinary Medicine, Knoxville, Tennessee, USA law9067@med.cornell.edu bemis@utk.edu.
authors.forename: David A
authors.initials: DA
authors.lastname: Bemis
mesh_terms: D000626:Aminopeptidases / Q000032:analysis*; D000818:Animals; D001431:Bacteriological Techniques / Q000379:methods; D006801:Humans; D013210:Staphylococcus / Q000145:classification / Q000201:enzymology* / Q000302:isolation & purification
chemical_list: D000626:Aminopeptidases; C043040:clostridial aminopeptidase
keywords: Staphylococcus delphini; Staphylococcus intermedius group; pyrrolidonyl arylamidase