title: Determination of Diphenylamine in Agricultural Products by HPLC-FL.
abstract: A method for the determination of diphenylamine in agricultural products was developed. Diphenylamine was extracted with acetonitrile from a sample under an acidic condition, passed through a C18 cartridge column, re-extracted with n-hexane, cleaned up on a PSA cartridge column, determined by HPLC with fluorescence detector and confirmed by liquid chromatography with tandem mass spectrometry. Average recoveries (n=5) from brown rice, corn, soybeans, potato, cabbage, eggplant, spinach, orange, apple and green tea were in the range from 76.7 to 94.9%, and the relative standard deviations were from 0.6 to 5.8% at concentrations equal to the maximum residue limits (MRLs). The quantification limits were 0.01 mg/kg, which is the uniform limit in the positive list system for agricultural chemical residues in food in Japan.
authors.affiliation: Hokkaido Institute of Public Health.
authors.forename: Mitsutoshi
authors.initials: M
authors.lastname: Aoyagi
authors.forename: Masahiro
authors.initials: M
authors.lastname: Chiba
authors.forename: Youichiro
authors.initials: Y
authors.lastname: Kakimoto
authors.forename: Satoru
authors.initials: S
authors.lastname: Nemoto
mesh_terms: D000097:Acetonitriles; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid; D018556:Crops, Agricultural / Q000737:chemistry*; D004159:Diphenylamine / Q000032:analysis* / Q000302:isolation & purification; D005453:Fluorescence; D005504:Food Analysis / Q000379:methods*; D006586:Hexanes; D010573:Pesticide Residues / Q000032:analysis* / Q000302:isolation & purification; D053719:Tandem Mass Spectrometry
chemical_list: D000097:Acetonitriles; D006586:Hexanes; D010573:Pesticide Residues; C026385:n-hexane; D004159:Diphenylamine; C032159:acetonitrile