title: Bio-active molecules modified surfaces enhanced mesenchymal stem cell adhesion and proliferation.
abstract: Surface modification of the substrate as a component of in vitro cell culture and tissue engineering, using bio-active molecules including extracellular matrix (ECM) proteins or peptides derived ECM proteins can modulate the surface properties and thereby induce the desired signaling pathways in cells. The aim of this study was to evaluate the behavior of human bone marrow mesenchymal stem cells (hBM-MSCs) on glass substrates modified with fibronectin (Fn), collagen (Coll), RGD peptides (RGD) and designed peptide (R-pept) as bio-active molecules. The glass coverslips were coated with fibronectin, collagen, RGD peptide and R-peptide. Bone marrow mesenchymal stem cells were cultured on different substrates and the adhesion behavior in early incubation times was investigated using scanning electron microscopy (SEM) and confocal microscopy. The MTT assay was performed to evaluate the effect of different bio-active molecules on MSCs proliferation rate during 24 and 72 h. Formation of filopodia and focal adhesion (FA) complexes, two steps of cell adhesion process, were observed in MSCs cultured on bio-active molecules modified coverslips, specifically in Fn coated and R-pept coated groups. SEM image showed well adhesion pattern for MSCs cultured on Fn and R-pept after 2 h incubation, while the shape of cells cultured on Coll and RGD substrates indicated that they might experience stress condition in early hours of culture. Investigation of adhesion behavior, as well as proliferation pattern, suggests R-peptide as a promising bio-active molecule to be used for surface modification of substrate in supporting and inducing cell adhesion and proliferation.
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran; Center for Nanofibers & Nanotechnology, Department of Mechanical Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Rezvan
authors.initials: R
authors.lastname: Mobasseri
authors.affiliation: Center for Nanofibers & Nanotechnology, Department of Mechanical Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Lingling
authors.initials: L
authors.lastname: Tian
authors.affiliation: Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: soleim_m@modares.ac.ir.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Center for Nanofibers & Nanotechnology, Department of Mechanical Engineering, National University of Singapore, 117576, Singapore; Guangdong-Hongkong-Macau Institute of CNS Regeneration (GHMICR), Jinan University, Guangzhou, 510632, China.
authors.forename: Seeram
authors.initials: S
authors.lastname: Ramakrishna
authors.affiliation: Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran. Electronic address: naderman@modares.ac.ir.
authors.forename: Hossein
authors.initials: H
authors.lastname: Naderi-Manesh
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology; D002448:Cell Adhesion; D049109:Cell Proliferation; D003094:Collagen / Q000737:chemistry; D005353:Fibronectins / Q000737:chemistry*; D022001:Focal Adhesions / Q000378:metabolism; D006493:Heparin / Q000737:chemistry; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D008855:Microscopy, Electron, Scanning; D008856:Microscopy, Fluorescence; D009842:Oligopeptides / Q000737:chemistry; D015398:Signal Transduction; D023822:Tissue Engineering / Q000379:methods
chemical_list: C067625:FN1 protein, human; D005353:Fibronectins; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D006493:Heparin; D003094:Collagen
keywords: Bio-active molecules; Cell adhesion; Mesenchymal stem cell; Peptide; Proliferation