title: In situ gelation properties of a collagen-genipin sol with a potential for the treatment of gastrointestinal ulcers.
abstract: We investigated the potential of collagen-genipin sols as biomaterials for treating artificial ulcers following endoscopic submucosal dissection. Collagen sol viscosity increased with condensation, allowing retention on tilted ulcers before gelation and resulting in collagen gel deposition on whole ulcers. The 1.44% collagen sols containing genipin as a crosslinker retained sol fluidity at 23°C for >20 min, facilitating endoscopic use. Collagen sols formed gel depositions on artificial ulcers in response to body temperature, and high temperature responsiveness of gelation because of increased neutral phosphate buffer concentration allowed for thick gel deposition on tilted ulcers. Finally, histological observations showed infiltration of gels into submucosal layers. Taken together, the present data show that genipin-induced crosslinking significantly improves the mechanical properties of collagen gels even at low genipin concentrations of 0.2-1 mM, warranting the use of in situ gelling collagen-genipin sols for endoscopic treatments of gastrointestinal ulcers.
authors.affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Koto-ku.
authors.forename: Takefumi
authors.initials: T
authors.lastname: Narita
authors.affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Koto-ku.
authors.forename: Shunji
authors.initials: S
authors.lastname: Yunoki
authors.affiliation: Biotechnology Group, Tokyo Metropolitan Industrial Technology Research Institute, Koto-ku.
authors.forename: Yoshimi
authors.initials: Y
authors.lastname: Ohyabu
authors.affiliation: Division of Research and Development for Minimally Invasive Treatment, Cancer Center, Keio University School of Medicine, Shinjuku-ku.
authors.forename: Naohisa
authors.initials: N
authors.lastname: Yahagi
authors.affiliation: Department of Gastroenterology, National Hospital Organization Tokyo Medical Center, Meguro-ku, Tokyo, Japan.
authors.forename: Toshio
authors.initials: T
authors.lastname: Uraoka
keywords: collagen; fibril formation; genipin; in-situ gel; ulcer dressing