title: Notch4 Signaling Pathway of Endothelial Progenitor Cells in a Kawasaki Disease Model Induced by Lactobacillus casei Cell Wall Extract.
abstract: The Notch4 signaling pathway of endothelial progenitor cells (EPCs) may play a crucial role in Kawasaki disease (KD). We investigated the proliferation, adhesion, migration, angiogenesis, and expression levels of Notch4, recombination signal-binding protein-Jκ (RBP-Jκ), P-selectin, and vascular cell adhesion molecule-1 (VCAM-1) of bone marrow (BM) EPCs in a KD model induced by Lactobacillus casei cell wall extract. The numbers of BM EPCs decreased significantly in the KD models. The Notch4 expression level on the EPC surface was higher in the KD models than in the controls. The proliferative, adhesive, migratory, and angiogenic properties, and double immunofluorescence-binding rate of BM EPCs were significantly impaired in the KD models. The levels of Notch4 and P-selectin mRNA were lower in the KD models than in the controls on day 3. The RBP-Jκ mRNA levels were lower in the KD models than in the controls on days 3 and 7. The levels of RBP-Jκ and vascular endothelial growth factor receptor-2 proteins decreased in the early stage. In conclusion, the BM EPC functions and bioactivities in the KD models were impaired, and the Notch4 signaling pathway is associated with KD.
authors.affiliation: The Children's Hospital of Zhejiang University School of Medicine, Hangzhou, PR China.
authors.forename: Huafeng
authors.initials: H
authors.lastname: Wang
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Xia
authors.forename: Songling
authors.initials: S
authors.lastname: Fu
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.forename: Chunhong
authors.initials: C
authors.lastname: Xie
authors.forename: Yiying
authors.initials: Y
authors.lastname: Zhang
authors.forename: Fangqi
authors.initials: F
authors.lastname: Gong
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D002465:Cell Movement; D049109:Cell Proliferation; D002473:Cell Wall; D002478:Cells, Cultured; D004195:Disease Models, Animal; D066026:Endothelial Progenitor Cells / Q000378:metabolism* / Q000473:pathology; D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein / Q000378:metabolism; D007780:Lacticaseibacillus casei; D008297:Male; D008807:Mice, Inbred BALB C; D009080:Mucocutaneous Lymph Node Syndrome / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D019007:P-Selectin / Q000378:metabolism; D010641:Phenotype; D011518:Proto-Oncogene Proteins / Q000235:genetics / Q000378:metabolism*; D000074562:Receptor, Notch4; D051880:Receptors, Notch / Q000235:genetics / Q000378:metabolism*; D015398:Signal Transduction; D013997:Time Factors; D019010:Vascular Cell Adhesion Molecule-1 / Q000378:metabolism; D040301:Vascular Endothelial Growth Factor Receptor-2 / Q000378:metabolism
chemical_list: D051819:Immunoglobulin J Recombination Signal Sequence-Binding Protein; D019007:P-Selectin; D011518:Proto-Oncogene Proteins; C497571:Rbpj protein, mouse; D000074562:Receptor, Notch4; D051880:Receptors, Notch; D019010:Vascular Cell Adhesion Molecule-1; C073572:Notch4 protein, mouse; C000592875:Kdr protein, mouse; D040301:Vascular Endothelial Growth Factor Receptor-2