title: Polyphenol Composition, Antioxidant Activity and Cytotoxicity of Seeds from Two Underexploited Wild Licania Species: L. rigida and L. tomentosa.
abstract: Studies have shown the benefit of antioxidants in the prevention or treatment of human diseases and promoted a growing interest in new sources of plant antioxidants for pharmacological use. This study aimed to add value to two underexploited wild plant species (Licania rigida)andL. tomentosa) from Brazilian flora. Thus, the phenolic compounds profile of their seed ethanol extract and derived fractions were elucidated by HPLC, the antioxidant capacity was assessed by in vitro chemical tests and the cytotoxicity determined using the human carcinoma cell lines MCF-7 and Caco-2. Eleven phenolic compounds were identified in the extracts of each species. The extracts and fractions showed excellent antioxidant activity in the DPPH assay (SC50, ranging from 9.15 to 248.8 µg/mL). The aqueous fraction ofL. rigidaseeds was most effective in preventing lipid peroxidation under basal conditions (IC5060.80 µg/mL) whereas, in the presence of stress inducer, the methanolic fraction ofL. tomentosaperformed best (IC508.55 µg/mL). None of the samples showed iron chelating capacity. Ethanolic seed extracts of both species did not reveal any cytotoxicity against MCF-7 and Caco-2 cells. Both plant species showed a promising phenolic profile with potent antioxidant capacity and deserve attention to be sustainably explored.
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil. igorppessoa@gmail.com.
authors.forename: Igor
authors.initials: I
authors.lastname: Parra Pessoa
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil. lopes.joaquimm@hotmail.com.
authors.forename: José Joaquim
authors.initials: JJ
authors.lastname: Lopes Neto
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil. thiago.urca@gmail.com.
authors.forename: Thiago
authors.initials: T
authors.lastname: Silva de Almeida
authors.affiliation: Department of Molecular Biology, Federal University of Paraíba, 58051-900 João Pessoa, PB, Brazil. davi@dbm.ufpb.br.
authors.forename: Davi
authors.initials: D
authors.lastname: Felipe Farias
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil. leorv2005@hotmail.com.
authors.forename: Leonardo Rogério
authors.initials: LR
authors.lastname: Vieira
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil. jackelinemedeiros@hotmail.com.
authors.forename: Jackeline
authors.initials: J
authors.lastname: Lima de Medeiros
authors.affiliation: Health Sciences Center, Federal University of Santa Maria, 97105-900 Santa Maria, RS, Brazil. alineboligon@hotmail.com.
authors.forename: Aline
authors.initials: A
authors.lastname: Augusti Boligon
authors.affiliation: RIKILT, Wageningen University and Research, P.O. Box 230, 6700 AE Wageningen, The Netherlands. ad.peijnenburg@wur.nl.
authors.forename: Ad
authors.initials: A
authors.lastname: Peijnenburg
authors.affiliation: Department of Finance, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil. lume1250@yahoo.com.br.
authors.forename: Ivan
authors.initials: I
authors.lastname: Castelar
authors.affiliation: Department of Biochemistry and Molecular Biology, Federal University of Ceará, 60020-181 Fortaleza, CE, Brazil. aurano@ufc.br.
authors.forename: Ana
authors.initials: A
authors.lastname: Fontenele Urano Carvalho
mesh_terms: D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000037:antagonists & inhibitors; D018938:Caco-2 Cells; D002470:Cell Survival / Q000187:drug effects; D045469:Chrysobalanaceae / Q000737:chemistry*; D005419:Flavonoids / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D061986:MCF-7 Cells; D000432:Methanol / Q000737:chemistry; D010636:Phenols / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D010851:Picrates / Q000037:antagonists & inhibitors; D010936:Plant Extracts / Q000737:chemistry; D012639:Seeds / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013634:Tannins / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D017392:Thiobarbituric Acid Reactive Substances / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D012997:Solvents; D013634:Tannins; D017392:Thiobarbituric Acid Reactive Substances; D014867:Water; C004931:1,1-diphenyl-2-picrylhydrazyl; D000432:Methanol
keywords: Caco-2; DPPH; MCF-7; TBARS; cytotoxicity; phenolic compound