title: New Application of pH-Mediated Acid Stacking Technique for Amphoteric Compounds in Capillary Electrophoresis: Example Assay of Blood Glutathiones.
abstract: Both reduced and oxidized forms of glutathiones were firstly stacked and detected using pH-mediated acid stacking method, in which glutathiones were stacked as cations and separated as anions. Factors, such as injection time, sweeping time, buffer pH, concentration of sodium chloride in sample matrix, that influenced stacking and separation were systematically studied and optimized. Under the optimum condition, the enhancement factors of ~20 times for both reduced and oxidized forms of glutathiones could be easily obtained within 20 min with satisfied sensitivities (limit of detections were 0.12 and 0.06 μmol/L for reduced and oxidized glutathione, respectively, at signal-to-noise ratio, S/N = 3), linearity range (0.3-300.0 and 0.6-300.0 μmol/L for reduced and oxidized glutathione, respectively), recoveries (>98%) and reproducibilities (relative standard deviation <5.1% for peak height). The proposed method provides an alternation way for assaying of glutathiones, as well as amphoteric compounds, in blood sample.
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Kong
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Linghong
authors.initials: L
authors.lastname: Kong
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Zhanwu
authors.initials: Z
authors.lastname: Hou
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Guifang
authors.initials: G
authors.lastname: Yang
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Hua
authors.initials: H
authors.lastname: Li
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Ji
authors.affiliation: The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Department of Biological Science and Engineering, School of Life Science and Technology, Institute of Mitochondrial Biology and Medicine,Xi'an Jiao Tong University, Xi'an, Shaanxi 710049, PR China.
authors.forename: Meili
authors.initials: M
authors.lastname: Gao
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000379:methods*; D005978:Glutathione / Q000097:blood* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D057230:Limit of Detection; D016014:Linear Models; D051379:Mice; D015203:Reproducibility of Results
chemical_list: D005978:Glutathione