title: Curcumin inhibits growth potential by G1 cell cycle arrest and induces apoptosis in p53-mutated COLO 320DM human colon adenocarcinoma cells.
abstract: Curcumin, a natural polyphenolic compound and it is isolated from the rhizome of Curcuma longa, have been reported to possess anticancer effect against stage I and II colon cancer. However, the effect of curcumin on colon cancer at Dukes' type C metastatic stage III remains still unclear. In the present study, we have investigated the anticancer effects of curcumin on p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage. The cellular viability and proliferation were assessed by trypan blue exclusion assay and MTT assay, respectively. The cytotoxicity effect was examined by lactate dehydrogenase (LDH) cytotoxicity assay. Apoptosis was analyzed by DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis. Cell cycle distribution was performed by flow cytometry analysis. Here we have observed that curcumin treatment significantly inhibited the cellular viability and proliferation potential of p53 mutated COLO 320DM cells in a dose- and time-dependent manner. In addition, curcumin treatment showed no cytotoxic effects to the COLO 320DM cells. DNA fragmentation analysis, Hoechst and propidium iodide double fluorescent staining and confocal microscopy analysis revealed that curcumin treatment induced apoptosis in COLO 320DM cells. Furthermore, curcumin caused cell cycle arrest at the G1 phase, decreased the cell population in the S phase and induced apoptosis in COLO 320DM colon adenocarcinoma cells. Together, these data suggest that curcumin exerts anticancer effects and induces apoptosis in p53 mutated COLO 320DM human colon adenocarcinoma cells derived from Dukes' type C metastatic stage.
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamilnadu, India.
authors.forename: Jade Dhananjay
authors.initials: JD
authors.lastname: Dasiram
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamilnadu, India.
authors.forename: Ramamoorthi
authors.initials: R
authors.lastname: Ganesan
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamilnadu, India.
authors.forename: Janani
authors.initials: J
authors.lastname: Kannan
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamilnadu, India.
authors.forename: Venkatesan
authors.initials: V
authors.lastname: Kotteeswaran
authors.affiliation: Department of Biotechnology, School of Bioengineering, SRM University, Kattankulathur 603203, Tamilnadu, India. Electronic address: nageswaran.s@ktr.srmuniv.ac.in.
authors.forename: Nageswaran
authors.initials: N
authors.lastname: Sivalingam
mesh_terms: D000230:Adenocarcinoma / Q000188:drug therapy* / Q000235:genetics; D017209:Apoptosis / Q000187:drug effects*; D059447:Cell Cycle Checkpoints / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000235:genetics; D003474:Curcumin / Q000494:pharmacology*; D053938:DNA Fragmentation / Q000187:drug effects; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects*; D006801:Humans; D009154:Mutation / Q000235:genetics; D016196:S Phase / Q000187:drug effects; D016159:Tumor Suppressor Protein p53 / Q000235:genetics*
chemical_list: C495901:TP53 protein, human; D016159:Tumor Suppressor Protein p53; D003474:Curcumin
keywords: Apoptosis; Cell cycle; Colon adenocarcinoma; Curcumin; Dukes’ type C metastatic stage; Tumor suppressor protein p53