title: Hybrid complexes of high and low molecular weight: evaluation using an in vitro model of osteoarthritis.
abstract: Hyaluronan (HA) is central in joint and cartilage functions and to restore synovial fluid viscosity. In patients with osteoarthritis (OA), molecular weight (MW) and concentration of hyaluronic acid (HA) are reduced, diminishing joint lubrication. IL-1β treatment was used to mimic osteoarthritis in a chondrocytes based in vitro model. The aim of our research, using this model and human chondrocytes was to assess the anti-inflammatory effect of H/L-HA hybrid complexes (SINOVIAL-HL®) in comparison with HA at high (H-HA) and low molecular weight (L-HA) separately used, through the evaluation of specific biomarkers involved in cartilage degradation and correlated to osteoarthritis. Specifically, TNF-α and IL-6 mRNA were evaluated by qRT-PCR. Cytokines levels were measured using Bio-plex assays and COMP-2 through immunofluorescence staining and western blot. H/L-HA significantly reduced inflammation biomarkers respect to both L-HA or H-HA separately considered at transcriptional and protein level.
authors.affiliation: Department of Experimental Medicine, Section of Biotechnology, Medical Histology and Molecular Biology, Second University of Naples, Naples, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Stellavato
authors.affiliation: Department of Experimental Medicine, Section of Biotechnology, Medical Histology and Molecular Biology, Second University of Naples, Naples, Italy.
authors.forename: F
authors.initials: F
authors.lastname: De Novellis
authors.affiliation: Department of Experimental Medicine, Section of Biotechnology, Medical Histology and Molecular Biology, Second University of Naples, Naples, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Reale
authors.affiliation: Department of Experimental Medicine, Section of Biotechnology, Medical Histology and Molecular Biology, Second University of Naples, Naples, Italy.
authors.forename: M
authors.initials: M
authors.lastname: De Rosa
authors.affiliation: Department of Experimental Medicine, Section of Biotechnology, Medical Histology and Molecular Biology, Second University of Naples, Naples, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Schiraldi
mesh_terms: D000893:Anti-Inflammatory Agents / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D002356:Cartilage / Q000473:pathology; D019902:Chondrocytes / Q000187:drug effects / Q000473:pathology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D066298:In Vitro Techniques; D007249:Inflammation / Q000188:drug therapy / Q000235:genetics / Q000378:metabolism; D053583:Interleukin-1beta / Q000009:adverse effects; D015850:Interleukin-6 / Q000235:genetics; D008954:Models, Biological; D008970:Molecular Weight; D010003:Osteoarthritis / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D014409:Tumor Necrosis Factor-alpha / Q000235:genetics
chemical_list: D000893:Anti-Inflammatory Agents; D053583:Interleukin-1beta; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D006820:Hyaluronic Acid