title: miR-520 promotes DNA-damage-induced trophoblast cell apoptosis by targeting PARP1 in recurrent spontaneous abortion (RSA).
abstract: The establishment and maintenance of successful pregnancy mainly depends on trophoblast cells. Their dysfunction has been implicated in recurrent spontaneous abortion (RSA), a major complication of pregnancy. However, the underlying mechanisms of trophoblasts dysfunction remain unclear. DNA-damage-induced cell apoptosis has been reported to play a vital role in cell death. In this study, we identified a novel microRNA (miR-520) in RSA progression via regulating trophoblast cell apoptosis. Microarray analysis showed that miR-520 was highly expressed in villus of RSA patients. By using flow cytometry analysis, we observed miR-520 expression was correlated with human trophoblast cell apoptosis in vitro, along with decreased poly (ADP-ribose) polymerase-1 (PARP1) expression. With the analysis of clinic samples, we observed that miR-520 level was negatively correlated with PARP1 level in RSA villus. In addition, overexpression of PARP1 restored the miR-520-induced trophoblast cell apoptosis in vitro. The status of chromosome in trophoblast implied that miR-520-promoted DNA-damage-induced cell apoptosis to regulate RSA progression. These results indicated that the level of miR-520 might associate with RSA by prompting trophoblast cell apoptosis via PARP1 dependent DNA-damage pathway.
authors.affiliation: a Department of Gynecology and Obstetrics , Cangzhou Center Hospital , Cangzhou , Hebei Province , P.R. China and.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Dong
authors.affiliation: a Department of Gynecology and Obstetrics , Cangzhou Center Hospital , Cangzhou , Hebei Province , P.R. China and.
authors.forename: Long
authors.initials: L
authors.lastname: Yang
authors.affiliation: b Department of Gynecology and Obstetrics , The First People's Hospital of Shangqiu , Shangqiu , Henan Province , P.R. China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000026:Abortion, Habitual / Q000235:genetics / Q000378:metabolism*; D000328:Adult; D017209:Apoptosis / Q000502:physiology*; D004249:DNA Damage; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D000071137:Poly (ADP-Ribose) Polymerase-1 / Q000235:genetics / Q000378:metabolism*; D011247:Pregnancy; D014327:Trophoblasts / Q000166:cytology / Q000378:metabolism*; D055815:Young Adult
chemical_list: C575048:MIRN520 microRNA, human; D035683:MicroRNAs; C499320:PARP1 protein, human; D000071137:Poly (ADP-Ribose) Polymerase-1
keywords: Apoptosis; DNA damage; PARP1; RSA; microRNA-520; recurrent spontaneous abortion; trophoblast