title: Self-guided reconstruction for time-domain fluorescence molecular lifetime tomography.
abstract: Fluorescence probes have distinct yields and lifetimes when located in different environments, which makes the reconstruction of fluorescence molecular lifetime tomography (FMLT) challenging. To enhance the reconstruction performance of time-domain (TD) FMLT with heterogeneous targets, a self-guided L 1 regularization projected steepest descent (SGL1PSD) algorithm is proposed. Different from other algorithms performed in time domain, SGL1PSD introduces a time-resolved strategy into fluorescence yield reconstruction. The algorithm consists of four steps. Step 1 reconstructs the initial yield map with full time gate strategy; steps 2–4 reconstruct the inverse lifetime map, the yield map, and the inverse lifetime map again with time-resolved strategy, respectively. The reconstruction result of each step is used as a priori for the reconstruction of the next step. Projected iterated Tikhonov regularization algorithm is adopted for the yield map reconstructions in steps 1 and 3 to provide a solution with iterative refinement and nonnegative constraint. The inverse lifetime map reconstructions in steps 2 and 4 are based on L 1 regularization projected steepest descent algorithm, which employ the L 1 regularization to reduce the ill-posedness of the high-dimensional nonlinear problem. Phantom experiments with heterogeneous targets at different edge-to-edge distances demonstrate that SG
authors.affiliation: Tsinghua University, School of Medicine, Department of Biomedical Engineering, Beijing 100084, China.
authors.forename: Chuangjian
authors.initials: C
authors.lastname: Cai
authors.affiliation: Tsinghua University, School of Medicine, Department of Biomedical Engineering, Beijing 100084, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Cai
authors.affiliation: Tsinghua University, School of Medicine, Department of Biomedical Engineering, Beijing 100084, China.
authors.forename: Jiaju
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Tsinghua University, School of Medicine, Department of Biomedical Engineering, Beijing 100084, China.
authors.forename: Yuxuan
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Tsinghua University, School of Medicine, Department of Biomedical Engineering, Beijing 100084, ChinabTsinghua University, Center for Biomedical Imaging Research, Beijing 100084, China.
authors.forename: Jianwen
authors.initials: J
authors.lastname: Luo
mesh_terms: D000465:Algorithms; D005456:Fluorescent Dyes; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D019047:Phantoms, Imaging; D041622:Tomography, Optical / Q000379:methods*
chemical_list: D005456:Fluorescent Dyes