title: [Value of Analyzing Hemoglobin A2by ROC Curve for Screening Thalassemia].
abstract: OBJECTIVE
  To investigate the value of hemoglobin A2(HbA2) for screening thalassemia.
  
  
  METHODS
  A total of 2 000 adults' peripheral blood samples from Guangdong Women and Children Hospital from June 2013 to January 2014 were collected. The hemoglobin A2(HbA2) level was analyzed by the full automatic capillary electrophoresis technique, and the genotypes of thalassemia were detected.
  
  
  RESULTS
  The optimal cutoff values of HbA2for screening silent α-thalassemia, α-thalassemia trait, intermedia α-thalassemia and β-thalassemia trait were 2.85%, 2.65%, 2.25% and 3.45%, respectively; the areas under receiver operator characteristic (ROC) curve were 0.709, 0.839, 0.979 and 0.997 respectively; the sensitivities were 0.481, 0.721, 0.953 and 0.994, and the specificities were 0.846, 0.837, 0.929 and 0.969 respectively.
  
  
  CONCLUSIONS
  The optimal cutoff values of HbA2for screening different type of thalassemia based on our laboratory data are established by using ROC curve. According to the area under ROC curve, a satisfactory accuracy for screening intermedia α-thalassemia and β-thalassemia trait can be achieved by detecting hemoglobin A2level.
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Tian-Wen
authors.initials: TW
authors.lastname: He
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Li-Hua
authors.initials: LH
authors.lastname: Yu
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Guo
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Yi-Xia
authors.initials: YX
authors.lastname: Wang
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Li
authors.initials: L
authors.lastname: DU
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Teng-Long
authors.initials: TL
authors.lastname: Yuan
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Yan-Xia
authors.initials: YX
authors.lastname: Zhang
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China.
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Luo
authors.affiliation: Medical Genetics Center, Key Laboratory of Metabolic and Genetic Diseases of Women and Children,Guangdong Women and Children Hospital, Guangzhou 511442, Guangdong Province, China. E-mail: yinaiwa@vip.126.com.
authors.forename: Ai-Hua
authors.initials: AH
authors.lastname: Yin
mesh_terms: D019075:Electrophoresis, Capillary; D005260:Female; D005838:Genotype; D006443:Hemoglobin A2; D006801:Humans; D008403:Mass Screening; D012372:ROC Curve; D013789:Thalassemia
chemical_list: D006443:Hemoglobin A2