title: Cross-Linked Polymer-Stabilized Nanocomposites for the Treatment of Bacterial Biofilms.
abstract: Infections caused by bacterial biofilms are an emerging threat to human health. Conventional antibiotic therapies are ineffective against biofilms due to poor penetration of the extracellular polymeric substance secreted by colonized bacteria coupled with the rapidly growing number of antibiotic-resistant strains. Essential oils are promising natural antimicrobial agents; however, poor solubility in biological conditions limits their applications against bacteria in both dispersed (planktonic) and biofilm settings. We report here an oil-in-water cross-linked polymeric nanocomposite (∼250 nm) incorporating carvacrol oil that penetrates and eradicates multidrug-resistant (MDR) biofilms. The therapeutic potential of these materials against challenging wound biofilm infections was demonstrated through specific killing of bacteria in a mammalian cell-biofilm coculture wound model.
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Ryan F
authors.initials: RF
authors.lastname: Landis
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Akash
authors.initials: A
authors.lastname: Gupta
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.|Department of Chemistry, Boğaziçi University , Bebek, Istanbul 34342, Turkey.
authors.forename: Bianka
authors.initials: B
authors.lastname: Golba
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.|Department of Chemistry, University of Pierre and Marie Curie , 4 Place Jussieu, Paris 75005, France.
authors.forename: Brice
authors.initials: B
authors.lastname: Couillaud
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.|Department of Polymer Science, Colloids and Formulation, ENSCBP - Bordeaux INP , 16 Avenue Pey Berland, Pessac 33607, France.
authors.forename: Roxane
authors.initials: R
authors.lastname: Ridolfo
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Riddha
authors.initials: R
authors.lastname: Das
authors.affiliation: Department of Chemistry, University of Massachusetts Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Vincent M
authors.identifier: 0000-0002-5184-5439
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D018441:Biofilms / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D003432:Cross-Linking Reagents / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D016972:Enterobacter cloacae / Q000187:drug effects / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D005347:Fibroblasts / Q000187:drug effects / Q000378:metabolism; D051379:Mice; D008826:Microbial Sensitivity Tests; D041681:NIH 3T3 Cells; D053761:Nanocomposites / Q000737:chemistry*; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology*; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects / Q000378:metabolism; D013329:Structure-Activity Relationship
chemical_list: D000900:Anti-Bacterial Agents; D003432:Cross-Linking Reagents; D011108:Polymers
keywords: biofilms; cross-linked; multidrug-resistant bacteria; nanocomposite; phytochemicals