title: 13C and15N chemical shift assignments of mammalian Y145Stop prion protein amyloid fibrils.
abstract: The Y145Stop prion protein (PrP23-144), which has been linked to the development of a heritable prionopathy in humans, is a valuable in vitro model for elucidating the structural and molecular basis of amyloid seeding specificities. Here we report the sequential backbone and side-chain13C and15N assignments of mouse and Syrian hamster PrP23-144 amyloid fibrils determined by using 2D and 3D magic-angle spinning solid-state NMR. The assigned chemical shifts were used to predict the secondary structures for the core regions of the mouse and Syrian hamster PrP23-144 amyloids, and the results compared to those for human PrP23-144 amyloid, which has previously been analyzed by solid-state NMR techniques.
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 222 CBEC Building, 151 West Woodruff Avenue, Columbus, OH, 43210, USA.
authors.forename: Theint
authors.initials: T
authors.lastname: Theint
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 222 CBEC Building, 151 West Woodruff Avenue, Columbus, OH, 43210, USA.
authors.forename: Philippe S
authors.initials: PS
authors.lastname: Nadaud
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH, 44106, USA.
authors.forename: Krystyna
authors.initials: K
authors.lastname: Surewicz
authors.affiliation: Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH, 44106, USA.
authors.forename: Witold K
authors.initials: WK
authors.lastname: Surewicz
authors.affiliation: Department of Chemistry and Biochemistry, The Ohio State University, 222 CBEC Building, 151 West Woodruff Avenue, Columbus, OH, 43210, USA. jaroniec.1@osu.edu.
authors.forename: Christopher P
authors.identifier: 0000-0003-0364-2888
authors.initials: CP
authors.lastname: Jaroniec
mesh_terms: D000595:Amino Acid Sequence; D000682:Amyloid / Q000737:chemistry*; D000818:Animals; D006224:Cricetinae; D051379:Mice; D019906:Nuclear Magnetic Resonance, Biomolecular; D010446:Peptide Fragments / Q000737:chemistry; D000072002:Prion Proteins / Q000737:chemistry*; D055503:Protein Multimerization; D017433:Protein Structure, Secondary
chemical_list: D000682:Amyloid; D010446:Peptide Fragments; D000072002:Prion Proteins
keywords: Amyloid; Magic-angle spinning; Prion protein; Solid-state NMR