title: Analysis of microRNA expression profile in specific pathogen-free chickens in response to reticuloendotheliosis virus infection.
abstract: Reticuloendotheliosis virus (REV) is an avian retrovirus that causes immunosuppression, growth retardation, and oncogenesis in a variety of birds. REV infection is epidemic in many countries. In this study, we used high-throughput sequencing to identify microRNAs (miRNAs) associated with REV infection. A total of 88 differentially expressed miRNAs were identified in samples collected on days 21 and 28 post-REV infection. Possible target genes of the differentially expressed miRNAs were analyzed. We observed that expression of proapoptotic, proto-oncogene, and carcinogenic cytokine mRNAs was highly upregulated, whereas expression of antiapoptotic cytokine mRNAs was significantly downregulated. Our findings provide a potential link between miRNA expression and the pathogenesis of REV infection.
authors.affiliation: Laboratory Pathological Physiology, College of Veterinary Medicine, Northeast Agricultural University, No.59 Mucai street, Harbin, 150030, People's Republic of China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: Yu
authors.affiliation: Laboratory Pathological Physiology, College of Veterinary Medicine, Northeast Agricultural University, No.59 Mucai street, Harbin, 150030, People's Republic of China.
authors.forename: Xueli
authors.initials: X
authors.lastname: Gao
authors.affiliation: Laboratory Pathological Physiology, College of Veterinary Medicine, Northeast Agricultural University, No.59 Mucai street, Harbin, 150030, People's Republic of China.
authors.forename: Chaonan
authors.initials: C
authors.lastname: Liu
authors.affiliation: Laboratory Pathological Physiology, College of Veterinary Medicine, Northeast Agricultural University, No.59 Mucai street, Harbin, 150030, People's Republic of China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Lv
authors.affiliation: Laboratory Pathological Physiology, College of Veterinary Medicine, Northeast Agricultural University, No.59 Mucai street, Harbin, 150030, People's Republic of China. zhengshiminbl@sohu.com.
authors.forename: Shimin
authors.initials: S
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000914:Antibodies, Viral; D017209:Apoptosis / Q000235:genetics; D002645:Chickens / Q000821:virology*; D016207:Cytokines / Q000235:genetics; D020869:Gene Expression Profiling; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D009857:Oncogenes; D011201:Poultry Diseases / Q000235:genetics* / Q000821:virology; D060888:Real-Time Polymerase Chain Reaction; D001356:Reticuloendotheliosis virus / Q000276:immunology / Q000472:pathogenicity / Q000502:physiology*; D012192:Retroviridae Infections / Q000235:genetics* / Q000821:virology; D013047:Specific Pathogen-Free Organisms; D014412:Tumor Virus Infections / Q000235:genetics* / Q000821:virology
chemical_list: D000914:Antibodies, Viral; D016207:Cytokines; D035683:MicroRNAs
keywords: Chicken; Gene expression; Reticuloendotheliosis virus; microRNA