title: Pancreatic lipase-related protein 2 is essential for egg hatching in the brown planthopper, Nilaparvata lugens.
abstract: In this study, we identified a pancreatic lipase-related protein 2 (PLRP2) gene through searching the transcriptome data of the brown planthopper, Nilaparvata lugens, a monophagous rice pest. PLRP2 mRNAs were first isolated in the human pancreas and play an important role in hydrolysis of galactolipids from the diet. Although homologous PLRP2 genes have been identified in many insect species, their physiological functions remain unknown. The present study for the first time reports the functional role of PLRP2 in an insect species. Differing from mammal PLRP2s, N. lugens PLRP2 was highly expressed in developing oocytes of the ovaries of female adults and little expressed in laid eggs. Suppression of N. lugens PLRP2 expression using RNA interference significantly inhibited egg hatching in rice seedlings, implying that N. lugens PLRP2 was important for oocyte maturation and development. This novel finding will improve our understanding of the reproductive strategies in insects and provides a potential target for future management of crop pests.
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University, Hangzhou, 310058, China.
authors.forename: L
authors.initials: L
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University, Hangzhou, 310058, China.
authors.forename: H-J
authors.initials: HJ
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University, Hangzhou, 310058, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University, Hangzhou, 310058, China.
authors.forename: C-W
authors.initials: CW
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Rice Biology and Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences Zhejiang University, Hangzhou, 310058, China.
authors.forename: Y-Y
authors.initials: YY
authors.lastname: Bao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D005260:Female; D006430:Hemiptera / Q000502:physiology*; D008049:Lipase / Q000235:genetics / Q000378:metabolism*; D008969:Molecular Sequence Data; D010058:Oviposition; D010063:Ovum / Q000502:physiology*
chemical_list: D008049:Lipase; C075988:pancreatic lipase related protein 2
keywords: RNA interference; brown planthopper Nilaparvata lugens; developing oocyte; egg hatching; ovary; pancreatic lipase-related protein 2 (PLRP2)