title: Proteomic insight into the primycin fermentation process of Saccharomonospora azurea.
abstract: Saccharomonospora azurea SZMC 14600 is a member of the family Pseudonocardiaceae exclusively used for industrial scale production of primycin a large 36-membered non-polyene macrolide lactone antibiotic belonging to the polyketide class of natural products. Even though maximum antibiotic yield has been achieved by empirically optimized two-step fermentation process, little is known about the molecular components and mechanisms underlying the efficient antibiotic production. In order to identify differentially expressed proteins (DEPs) between the pre- and main-fermentation stages of primycin, comparative 2D-PAGE experiments were performed. In total, 98 DEP spots were reproducibly detected, out of which four spots were excised from gels, and identified through MALDI-TOF/TOF mass spectrometry. Peptide mass fingerprint analysis revealed peptide matches to HicB antitoxin for the HicAB toxin-antitoxin system (EHK86651), to a nucleoside diphosphate kinase regulator ((Ndk; EHK81899) and two other proteins with unknown function (EHK88946 and EHK86777).
authors.affiliation: Szentágothai Research Centre, University of Pécs , Pécs , Hungary.
authors.forename: Andrea
authors.initials: A
authors.lastname: Valasek
authors.affiliation: Szentágothai Research Centre, University of Pécs , Pécs , Hungary.|Department of General and Environmental Microbiology, University of Pécs , Pécs , Hungary.
authors.forename: Írisz Éva
authors.initials: ÍÉ
authors.lastname: Kiss
authors.affiliation: Szentágothai Research Centre, University of Pécs , Pécs , Hungary.|Department of General and Environmental Microbiology, University of Pécs , Pécs , Hungary.
authors.forename: István
authors.initials: I
authors.lastname: Fodor
authors.affiliation: Szentágothai Research Centre, University of Pécs , Pécs , Hungary.|Department of General and Environmental Microbiology, University of Pécs , Pécs , Hungary.
authors.forename: Márk
authors.initials: M
authors.lastname: Kovács
authors.affiliation: Szentágothai Research Centre, University of Pécs , Pécs , Hungary.|Department of General and Environmental Microbiology, University of Pécs , Pécs , Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Urbán
authors.affiliation: Department of Biochemistry and Medical Chemistry, University of Pécs , Pécs , Hungary.
authors.forename: Éva
authors.initials: É
authors.lastname: Jámbor
authors.affiliation: Szentágothai Research Centre, University of Pécs , Pécs , Hungary.|Department of General and Environmental Microbiology, University of Pécs , Pécs , Hungary.
authors.forename: Csaba
authors.initials: C
authors.lastname: Fekete
authors.affiliation: Department of General and Environmental Microbiology, University of Pécs , Pécs , Hungary.
authors.forename: Ildikó
authors.initials: I
authors.lastname: Kerepesi
mesh_terms: D000192:Actinomycetales / Q000378:metabolism*; D015180:Electrophoresis, Gel, Two-Dimensional; D005285:Fermentation; D018942:Macrolides / Q000378:metabolism*; D020543:Proteome / Q000378:metabolism*; D040901:Proteomics; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D018942:Macrolides; D020543:Proteome; C009041:primycin
keywords: Differentially expressed proteins; HicB-family protein; Saccharomonospora azurea; primycin; two-dimensional protein gel electrophoresis