title: Mucin secreting cells in the stomach and colon are altered by combination antiretroviral treatment in an obese rat model.
abstract: Mucins, secreted by intestinal goblet cells, form an integral part of the intestinal biofilm, which is important for the functioning of a healthy gastrointestinal tract (GIT). This mucous layer is sensitive to factors such as diet, drugs and inflammation. Histochemically, mucins can be classified as neutral or acidic, where acidic mucins can contain sulphate groups (sulphomucins) or sialic acid (sialomucins). The aim of the present study was to determine the composition of various mucin secreting cells using histochemical stains in rats fed on a high calorie diet (HCD) treated with antiretroviral therapy (ART). Wistar rats (N=24) were divided into a lean control group (C/ART-), high calorie diet group (C/HCD+), ART group (C/ART+) and HCD and ART group (HCD+/ART+). The body of the stomach as well as the colon were stained with Alcian Blue Periodic Schiff (ABPAS) to distinguish between neutral and acidic mucins and Alcian Blue Aldehyde Fuschin (ABAF) to distinguish between sialo-and sulphomucins. An increase of the total gastric mucous cells was observed in the HCD+/ART+ group compared to the C/ART- group using both ABPAS and ABAF. A decrease of neutral cells in the distal part of the colonic crypts in the C/HCD+ and C/ART+ groups compared to the C/ART- group were observed. Mixed goblet cells in the colonic crypts of the C/ART- and HCD+/ART+ groups were decreased in comparison to the C/ART+ group. The study showed that the total mean percentage of mucous cells in the stomach as well as the total amount of neutral goblet cells in the colon were most affected by ART and a HCD. These changes in a rat model suggest that the quality of the biofilm may be altered and should be considered when ART is prescribed to obese patients.
authors.affiliation: Division of Anatomy and Histology, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, South Africa.
authors.forename: Danélle
authors.initials: D
authors.lastname: Truter
authors.affiliation: Division of Medical Physiology, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, South Africa.
authors.forename: Hans
authors.initials: H
authors.lastname: Strijdom
authors.affiliation: Division of Medical Physiology, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, South Africa.
authors.forename: Frans
authors.initials: F
authors.lastname: Everson
authors.affiliation: Division of Anatomy and Histology, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Stellenbosch University, PO Box 241, Cape Town, South Africa. Electronic address: shk@sun.ac.za.
authors.forename: Sanet H
authors.initials: SH
authors.lastname: Kotzé
mesh_terms: D000818:Animals; D044966:Anti-Retroviral Agents / Q000494:pharmacology*; D003106:Colon / Q000378:metabolism / Q000473:pathology; D004353:Drug Evaluation, Preclinical; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D008297:Male; D009077:Mucins / Q000378:metabolism*; D009765:Obesity / Q000378:metabolism* / Q000473:pathology; D017208:Rats, Wistar; D013270:Stomach / Q000473:pathology
chemical_list: D044966:Anti-Retroviral Agents; D009077:Mucins
keywords: Antiretroviral treatment; Biofilm; Gastrointestinal tract; Histochemistry; Mucins; Obesity