title: Age-related arterial immune cell infiltration in mice is attenuated by caloric restriction or voluntary exercise.
abstract: Age-related arterial inflammation is associated with dysfunction of the arteries and increased risk for cardiovascular disease. To determine if aging increases arterial immune cell infiltration as well as the populations of immune cells principally involved, we tested the hypothesis that large elastic and resistance arteries in old mice would exhibit increased immune cell infiltration compared to young controls. Additionally, we hypothesized that vasoprotective lifestyle interventions such as lifelong caloric restriction or 8weeks of voluntary wheel running would attenuate age-related arterial immune cell infiltration. The aorta and mesenteric vasculature with surrounding perivascular adipose was excised from young normal chow (YNC, 4-6months, n=10), old normal chow (ONC, 28-29months, n=11), old caloric restricted (OCR, 28-29months, n=9), and old voluntary running (OVR, 28-29months, n=5) mice and digested to a single cell suspension. The cells were then labeled with antibodies against CD45 (total leukocytes), CD3 (pan T cells), CD4 (T helper cells), CD8 (cytotoxic T cells), CD19 (B cells), CD11b, and F4/80 (macrophages) and analyzed by flow cytometry. Total leukocytes, T cells (both CD4+and CD8+subsets), B cells, and macrophages in both aorta and mesentery were all 5- to 6-fold greater in ONC compared to YNC. Age-related increases in T cell (both CD4+and CD8+), B cell, and macrophage infiltration in aorta were abolished in OCR mice. OVR mice exhibited 50% lower aortic T cell and normalized macrophage infiltration. B cell infiltration was not affected by VR. Age-related mesenteric CD8+T cell and macrophage infiltration was normalized in OCR and OVR mice compared to young mice, whereas B cell infiltration was normalized by CR but not VR. Splenic CD4+T cells from ONC mice exhibited a 3-fold increase in gene expression for the T helper (Th) 1 transcription factor, Tbet, and a 4-fold increase in FoxP3, a T regulatory cell transcription factor, compared to YNC. Splenic B cells and mesenteric macrophages from old mice exhibited decreased proinflammatory cytokine gene expression regardless of treatment group. These results demonstrate that aging is associated with infiltration of immune cells around both the large-elastic and resistance arteries and that the vasoprotective lifestyle interventions, CR and VR, can ameliorate age-related arterial immune cell infiltration.
authors.affiliation: Department of Internal Medicine, Division of Geriatrics, University of Utah, Salt Lake City, UT, USA. Electronic address: daniel.trott@utah.edu.
authors.forename: Daniel W
authors.initials: DW
authors.lastname: Trott
authors.affiliation: Department of Exercise and Sport Science, University of Utah, Salt Lake City, UT, USA.
authors.forename: Grant D
authors.initials: GD
authors.lastname: Henson
authors.affiliation: Department of Internal Medicine, Division of Geriatrics, University of Utah, Salt Lake City, UT, USA.
authors.forename: Mi H T
authors.initials: MHT
authors.lastname: Ho
authors.affiliation: Department of Internal Medicine, Division of Geriatrics, University of Utah, Salt Lake City, UT, USA.
authors.forename: Sheilah A
authors.initials: SA
authors.lastname: Allison
authors.affiliation: Department of Internal Medicine, Division of Geriatrics, University of Utah, Salt Lake City, UT, USA; Department of Exercise and Sport Science, University of Utah, Salt Lake City, UT, USA; Geriatric Research, Education, and Clinical Center,Veterans Affairs Medical Center, Salt Lake City, UT, USA.
authors.forename: Lisa A
authors.initials: LA
authors.lastname: Lesniewski
authors.affiliation: Department of Internal Medicine, Division of Geriatrics, University of Utah, Salt Lake City, UT, USA; Department of Exercise and Sport Science, University of Utah, Salt Lake City, UT, USA; Geriatric Research, Education, and Clinical Center,Veterans Affairs Medical Center, Salt Lake City, UT, USA; Department of Biochemistry,University of Utah, Salt Lake City, UT, USA.
authors.forename: Anthony J
authors.initials: AJ
authors.lastname: Donato
mesh_terms: D000375:Aging / Q000276:immunology*; D000818:Animals; D001158:Arteries / Q000276:immunology*; D031204:Caloric Restriction; D007962:Leukocytes / Q000502:physiology; D008264:Macrophages / Q000502:physiology; D008297:Male; D051379:Mice; D010805:Physical Conditioning, Animal; D014652:Vascular Diseases / Q000209:etiology
keywords: Aorta; B cells; Inflammation; Macrophages; Mesentery; T cells