title: An LRR/Malectin Receptor-Like Kinase Mediates Resistance to Non-adapted and Adapted Powdery Mildew Fungi in Barley and Wheat.
abstract: Pattern recognition receptors (PRRs) belonging to the multigene family of receptor-like kinases (RLKs) are the sensing devices of plants for microbe- or pathogen-associated molecular patterns released from microbial organisms. Here we describeRnr8(forRequired for non-host resistance 8) encoding HvLEMK1, a LRR-malectin domain-containing transmembrane RLK that mediates non-host resistance of barley to the non-adapted wheat powdery mildew fungusBlumeria graminisf.sp.tritici. Transgenic barley lines with silencedHvLEMK1allow entry and colony growth of the non-adapted pathogen, although sporulation was reduced and final colony size did not reach that of the adapted barley powdery mildew fungusB. graminisf.sp.hordei. Transient expression of the barley or wheatLEMK1genes enhanced resistance in wheat to the adapted wheat powdery mildew fungus while expression of the same genes did not protect barley from attack by the barley powdery mildew fungus. The results suggest thatHvLEMK1is a factor mediating non-host resistance in barley and quantitative host resistance in wheat to the wheat powdery mildew fungus.
authors.affiliation: Pathogen-Stress Genomics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Stadt Seeland, Germany.
authors.forename: Jeyaraman
authors.initials: J
authors.lastname: Rajaraman
authors.affiliation: Pathogen-Stress Genomics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Stadt Seeland, Germany.
authors.forename: Dimitar
authors.initials: D
authors.lastname: Douchkov
authors.affiliation: Plant Reproductive Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Stadt Seeland, Germany.
authors.forename: Götz
authors.initials: G
authors.lastname: Hensel
authors.affiliation: National Institute of Agricultural Botany Cambridge, UK.
authors.forename: Francesca L
authors.initials: FL
authors.lastname: Stefanato
authors.affiliation: National Institute of Agricultural Botany Cambridge, UK.
authors.forename: Anna
authors.initials: A
authors.lastname: Gordon
authors.affiliation: National Institute of Agricultural Botany Cambridge, UK.
authors.forename: Nelzo
authors.initials: N
authors.lastname: Ereful
authors.affiliation: Department of Bioinformatics and Structural Biochemistry, Institute of Biochemistry of the Romanian Academy Bucharest, Romania.
authors.forename: Octav F
authors.initials: OF
authors.lastname: Caldararu
authors.affiliation: Department of Bioinformatics and Structural Biochemistry, Institute of Biochemistry of the Romanian Academy Bucharest, Romania.
authors.forename: Andrei-Jose
authors.initials: AJ
authors.lastname: Petrescu
authors.affiliation: Plant Reproductive Biology, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Stadt Seeland, Germany.
authors.forename: Jochen
authors.initials: J
authors.lastname: Kumlehn
authors.affiliation: National Institute of Agricultural Botany Cambridge, UK.
authors.forename: Lesley A
authors.initials: LA
authors.lastname: Boyd
authors.affiliation: Pathogen-Stress Genomics, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Stadt Seeland, Germany.
authors.forename: Patrick
authors.initials: P
authors.lastname: Schweizer
keywords: 3D model; Blumeria graminis; Hordeum vulgare; PRR; Triticum aestivum; co-evolution; pathogen recognition receptor