title: A predicted cation transporter protein, BPSS1228, is involved in intracellular behaviour of Burkholderia pseudomallei in a human lung epithelial cell line (A549).
abstract: Burkholderia pseudomallei causes melioidosis, a potentially fatal infectious disease in tropical and subtropical countries worldwide. The intracellular behaviour of this pathogen in host cells has been reported to impact the severity of melioidosis, including the development of septicaemia, a consequence of pneumonia melioidosis. We previously identified a predicted cation transporter protein, BPSS1228, that participates in the transitional stage of this intracellular pathogen. For further analysis, in this study B. pseudomallei bpss1228 mutant and complemented strains were constructed and bacterial infectivity on human lung epithelial cells, A549, investigated in vitro Burkholderia pseudomallei bpss1228 mutant showed impaired bacterial adhesion and invasion into A549 cells compared with wild-type strain, while the deficient phenotypes were restored to wild-type levels by the complemented strain. Additionally, the inactivation of bpss1228 in the mutant strain affected flagella-based swimming on a semi-solid surface and resistance to acid stresses simulating intracellular environments. These observations of BPSS1228 relating to B. pseudomallei infection strategies shed a new light on its association with intracellular B. pseudomallei during the interaction with host cells.
authors.affiliation: Department of Microbiology, Faculty of Medicine, Melioidosis Research Center and Biofilm Research Group, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Teerasit
authors.initials: T
authors.lastname: Techawiwattanaboon
authors.affiliation: Department of Microbiology, Faculty of Medicine, Melioidosis Research Center and Biofilm Research Group, Khon Kaen University, Khon Kaen, Thailand sorujsr@kku.ac.th.
authors.forename: Sorujsiri
authors.initials: S
authors.lastname: Chareonsudjai
mesh_terms: D000072283:A549 Cells; D001422:Bacterial Adhesion / Q000235:genetics*; D001426:Bacterial Proteins / Q000235:genetics*; D016957:Burkholderia pseudomallei / Q000235:genetics / Q000254:growth & development / Q000472:pathogenicity*; D027682:Cation Transport Proteins / Q000235:genetics*; D002460:Cell Line; D004847:Epithelial Cells / Q000166:cytology* / Q000382:microbiology; D005407:Flagella / Q000235:genetics* / Q000378:metabolism; D054884:Host-Pathogen Interactions / Q000235:genetics; D006801:Humans; D007473:Ion Channels / Q000378:metabolism; D008168:Lung / Q000166:cytology; D008554:Melioidosis / Q000382:microbiology / Q000473:pathology*; D011014:Pneumonia / Q000382:microbiology / Q000473:pathology; D020545:Respiratory Mucosa / Q000166:cytology*; D015854:Up-Regulation / Q000235:genetics
chemical_list: C000624627:BPSS1228 protein, Burkholderia pseudomallei; D001426:Bacterial Proteins; D027682:Cation Transport Proteins; D007473:Ion Channels
keywords: Burkholderia pseudomallei; bpss1228; predicted cation transporter protein