title: Identification of a New Cotton Disease Caused by an Atypical Cotton Leafroll Dwarf Virus in Argentina.
abstract: An outbreak of a new disease occurred in cotton (Gossypium hirsutum) fields in northwest Argentina starting in the 2009-10 growing season and is still spreading steadily. The characteristic symptoms of the disease included slight leaf rolling and a bushy phenotype in the upper part of the plant. In this study, we determined the complete nucleotide sequences of two independent virus genomes isolated from cotton blue disease (CBD)-resistant and -susceptible cotton varieties. This virus genome comprised 5,866 nucleotides with an organization similar to that of the genus Polerovirus and was closely related to cotton leafroll dwarf virus, with protein identity ranging from 88 to 98%. The virus was subsequently transmitted to a CBD-resistant cotton variety using Aphis gossypii and symptoms were successfully reproduced. To study the persistence of the virus, we analyzed symptomatic plants from CBD-resistant varieties from different cotton-growing fields between 2013 and 2015 and showed the presence of the same virus strain. In addition, a constructed full-length infectious cDNA clone from the virus caused disease symptoms in systemic leaves of CBD-resistant cotton plants. Altogether, the new leafroll disease in CBD-resistant cotton plants is caused by an atypical cotton leafroll dwarf virus.
authors.affiliation: First author: INTA-CICVyA, CONICET, Instituto de Biotecnología, 1686 Buenos Aires; second author: INTA-CICVyA, CONICET, Instituto de Biotecnología and School of Science and Technology, UNSAM, 1653 Buenos Aires; third and fifth authors: EEA Sáenz Peña, INTA, 3700 Chaco, Argentina; and fourth and sixth authors: INTA-CICVyA, Instituto de Biotecnología and DFBMC, FCEyN, UBA, 1428 Buenos Aires.
authors.forename: Yamila C
authors.initials: YC
authors.lastname: Agrofoglio
authors.affiliation: First author: INTA-CICVyA, CONICET, Instituto de Biotecnología, 1686 Buenos Aires; second author: INTA-CICVyA, CONICET, Instituto de Biotecnología and School of Science and Technology, UNSAM, 1653 Buenos Aires; third and fifth authors: EEA Sáenz Peña, INTA, 3700 Chaco, Argentina; and fourth and sixth authors: INTA-CICVyA, Instituto de Biotecnología and DFBMC, FCEyN, UBA, 1428 Buenos Aires.
authors.forename: Verónica C
authors.initials: VC
authors.lastname: Delfosse
authors.affiliation: First author: INTA-CICVyA, CONICET, Instituto de Biotecnología, 1686 Buenos Aires; second author: INTA-CICVyA, CONICET, Instituto de Biotecnología and School of Science and Technology, UNSAM, 1653 Buenos Aires; third and fifth authors: EEA Sáenz Peña, INTA, 3700 Chaco, Argentina; and fourth and sixth authors: INTA-CICVyA, Instituto de Biotecnología and DFBMC, FCEyN, UBA, 1428 Buenos Aires.
authors.forename: María F
authors.initials: MF
authors.lastname: Casse
authors.affiliation: First author: INTA-CICVyA, CONICET, Instituto de Biotecnología, 1686 Buenos Aires; second author: INTA-CICVyA, CONICET, Instituto de Biotecnología and School of Science and Technology, UNSAM, 1653 Buenos Aires; third and fifth authors: EEA Sáenz Peña, INTA, 3700 Chaco, Argentina; and fourth and sixth authors: INTA-CICVyA, Instituto de Biotecnología and DFBMC, FCEyN, UBA, 1428 Buenos Aires.
authors.forename: Horacio E
authors.initials: HE
authors.lastname: Hopp
authors.affiliation: First author: INTA-CICVyA, CONICET, Instituto de Biotecnología, 1686 Buenos Aires; second author: INTA-CICVyA, CONICET, Instituto de Biotecnología and School of Science and Technology, UNSAM, 1653 Buenos Aires; third and fifth authors: EEA Sáenz Peña, INTA, 3700 Chaco, Argentina; and fourth and sixth authors: INTA-CICVyA, Instituto de Biotecnología and DFBMC, FCEyN, UBA, 1428 Buenos Aires.
authors.forename: Iván Bonacic
authors.initials: IB
authors.lastname: Kresic
authors.affiliation: First author: INTA-CICVyA, CONICET, Instituto de Biotecnología, 1686 Buenos Aires; second author: INTA-CICVyA, CONICET, Instituto de Biotecnología and School of Science and Technology, UNSAM, 1653 Buenos Aires; third and fifth authors: EEA Sáenz Peña, INTA, 3700 Chaco, Argentina; and fourth and sixth authors: INTA-CICVyA, Instituto de Biotecnología and DFBMC, FCEyN, UBA, 1428 Buenos Aires.
authors.forename: Ana J
authors.initials: AJ
authors.lastname: Distéfano
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology*; D016679:Genome, Viral / Q000235:genetics*; D003368:Gossypium / Q000821:virology*; D052679:Luteoviridae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010935:Plant Diseases / Q000821:virology*