title: In vitro embryo production in wood bison (Bison bison athabascae) using in vivo matured cumulus-oocyte complexes.
abstract: Experiments were conducted in wood bison to determine the effect of additional maturation time on embryo development of in vivo matured oocytes. In experiment 1, cumulus-oocyte complexes (COC) were collected 30 hours after hCG treatment in superstimulated wood bison, and expanded COC were fertilized immediately or after 4 hours of additional in vitro maturation. Embryo development was assessed on Days 3, 7, and 8 (Day 0 = day of fertilization). No difference in cleavage rate was detected (55.3% vs. 60.5%, P = 0.82), but the Day 8 blastocyst rate was higher after an additional 4 hours of in vitro maturation time (44.7 vs. 18.4%, P = 0.03). In experiment 2, COC were collected at either 30 hours or 34 hours after hCG treatment. Expanded COC from the 30 hours group were fertilized after 4 hours of in vitro maturation, whereas those from the 34 hours group were fertilized immediately. A higher cleavage rate (74.3 vs. 57.0%) and blastocyst rate (54.1 vs. 37.2%) were found in the 34 hours group versus the 30 hours group (P < 0.05). In conclusion, an additional short period of in vitro maturation, or an extended period of in vivo maturation are beneficial for in vitro embryo production in wood bison.
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Miriam P
authors.initials: MP
authors.lastname: Cervantes
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada.
authors.forename: J Manuel
authors.initials: JM
authors.lastname: Palomino
authors.affiliation: Agriculture and Agri-Food Canada, Saskatoon Research and Development Center, Saskatoon, Canada.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Anzar
authors.affiliation: Department of Large Animal Clinical Sciences, University of Saskatchewan, Saskatoon, Canada.
authors.forename: Reuben J
authors.initials: RJ
authors.lastname: Mapletoft
authors.affiliation: Reproductive Physiology, Toronto Zoo, Toronto, Ontario, Canada.
authors.forename: Gabriela F
authors.initials: GF
authors.lastname: Mastromonaco
authors.affiliation: Department of Veterinary Biomedical Sciences, University of Saskatchewan, Saskatoon, Canada. Electronic address: gregg.adams@usask.ca.
authors.forename: Gregg P
authors.initials: GP
authors.lastname: Adams
mesh_terms: D000818:Animals; D016164:Bison / Q000502:physiology*; D001755:Blastocyst / Q000502:physiology; D047108:Embryonic Development / Q000502:physiology*; D005260:Female; D005307:Fertilization in Vitro / Q000662:veterinary; D059471:In Vitro Oocyte Maturation Techniques / Q000662:veterinary; D009865:Oocytes / Q000502:physiology*; D009866:Oogenesis / Q000502:physiology*; D013997:Time Factors
keywords: Blastocyst; In vitro; In vitro fertilization; In vivo maturation; Oocyte