title: A single and two step isomerization process for d-tagatose and l-ribose bioproduction using l-arabinose isomerase and d-lyxose isomerase.
abstract: l-ribose and d-tagatose are biochemically synthesized using sugar isomerases. The l-arabinose isomerase gene from Shigella flexneri (Sf-AI) was cloned and expressed in Escherichia coli BL-21. Sf-AI was applied for the bioproduction of d-tagatose from d-galactose. l-ribose synthesis was performed by two step isomerization using Sf-AI and d-lyxose/ribose isomerase from Cohnella laevoribosii. The overall 22.3% and 25% conversion rate were observed for d-tagatose and l-ribose production from d-galactose and l-arabinose respectively. In the present manuscript, synthesis of rare sugars from naturally available sugars is discussed along with the biochemical characterization of Sf-AI and its efficiency.
authors.affiliation: Department of Biochemistry, P. D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388 421, Gujarat, India.
authors.forename: Manisha J
authors.initials: MJ
authors.lastname: Patel
authors.affiliation: Department of Biochemistry, P. D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388 421, Gujarat, India.
authors.forename: Rekha C
authors.initials: RC
authors.lastname: Akhani
authors.affiliation: Department of Biochemistry, P. D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388 421, Gujarat, India.
authors.forename: Arti T
authors.initials: AT
authors.lastname: Patel
authors.affiliation: Department of Biochemistry, P. D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388 421, Gujarat, India.
authors.forename: Samir R
authors.initials: SR
authors.lastname: Dedania
authors.affiliation: Department of Biochemistry, P. D. Patel Institute of Applied Sciences, CHARUSAT, Changa, 388 421, Gujarat, India; Dr. K.C.Patel Research and Development Center, CHARUSAT, Changa, 388 421, Gujarat, India. Electronic address: darshanpatel.bt@charusat.ac.in.
authors.forename: Darshan H
authors.initials: DH
authors.lastname: Patel
mesh_terms: D019747:Aldose-Ketose Isomerases / Q000235:genetics / Q000378:metabolism*; D001089:Arabinose / Q000378:metabolism; D056449:Bacillales / Q000201:enzymology / Q000235:genetics; D001709:Biotechnology; D003001:Cloning, Molecular; D004795:Enzyme Stability; D005690:Galactose / Q000378:metabolism; D005798:Genes, Bacterial; D006601:Hexoses / Q000096:biosynthesis* / Q000737:chemistry; D007700:Kinetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D012266:Ribose / Q000096:biosynthesis* / Q000737:chemistry; D012763:Shigella flexneri / Q000201:enzymology / Q000235:genetics; D013237:Stereoisomerism
chemical_list: D006601:Hexoses; D011994:Recombinant Proteins; D012266:Ribose; D001089:Arabinose; D019747:Aldose-Ketose Isomerases; C021525:D-lyxose ketol-isomerase; C019715:L-arabinose isomerase; C030192:tagatose; D005690:Galactose
keywords: Shigella flexneri; Two step isomerization; d-Tagatose; l-Ribose; l-arabinose isomerase