title: A six-microRNA panel in plasma was identified as a potential biomarker for lung adenocarcinoma diagnosis.
abstract: Differently expressed microRNAs (miRNAs) in the plasma of lung adenocarcinoma (LA) patients might serve as biomarkers for LA detection. MiRNA expression profiling was performed using Exiqon panels followed by the verification (30 LA VS. 10 healthy controls (HCs)) with quantitative reverse transcription polymerase chain reaction (qRT-PCR) in the screening phase. Identified miRNAs were confirmed through training (42 LA VS. 32 HCs) and testing stages (66 LA VS. 62 HCs) by using qRT-PCR based absolute quantification methods. A total of six up-regulated plasma miRNAs (miR-19b-3p, miR-21-5p, miR-221-3p, miR-409-3p, miR-425-5p and miR-584-5p) were identified. The six-miRNA panel could discriminate LA patients from HCs with areas under the receiver operating characteristic curve of 0.72, 0.74 and 0.84 for the training, testing and the external validation stage (33 LA VS. 30 HCs), respectively. All the miRNAs identified except miR-584-5p were significantly up-regulated in LA tissues. MiR-19-3p, miR-21-5p, miR-409-3p and miR-425-5p showed high expression in arterial plasma with borderline significance. Additionally, miR-19-3p, miR-21-5p and miR-221-3p were significantly up-regulated in exosomes extracted from LA peripheral plasma samples. In conclusion, we identified a six-miRNA panel in peripheral plasma which might give assistance to the detection of LA at least for Asian population to a certain extent.
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Thoracic Surgery, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wen
authors.affiliation: Department of Respiration, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing 210000, PR China.
authors.forename: Xia
authors.initials: X
authors.lastname: Shan
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Renhua
authors.initials: R
authors.lastname: Guo
authors.affiliation: Department of Gastroenterology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Wenfang
authors.initials: W
authors.lastname: Cheng
authors.affiliation: Department of Cardiology, First Affiliated Hospital of Nanjing Medical University,Nanjing 210029, PR China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Natural Medicines and Department of Pharmacognosy, China Pharmaceutical University, Nanjing, 210009, China.
authors.forename: Lian-Wen
authors.initials: LW
authors.lastname: Qi
authors.affiliation: Department of Emergency, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Zebo
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.
authors.forename: Tongshan
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China.
authors.forename: Danxia
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.|Cancer Center of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Oncology, First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, PR China.|Cancer Center of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Yongqian
authors.initials: Y
authors.lastname: Shu
mesh_terms: D000230:Adenocarcinoma / Q000097:blood / Q000175:diagnosis* / Q000208:ethnology / Q000235:genetics*; D000077192:Adenocarcinoma of Lung; D019540:Area Under Curve; D044466:Asian People / Q000235:genetics; D014408:Biomarkers, Tumor / Q000097:blood / Q000235:genetics*; D016022:Case-Control Studies; D002681:China; D000074166:Circulating MicroRNA / Q000097:blood / Q000235:genetics*; D005260:Female; D020869:Gene Expression Profiling / Q000379:methods*; D020022:Genetic Predisposition to Disease; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood / Q000175:diagnosis* / Q000208:ethnology / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D010641:Phenotype; D011237:Predictive Value of Tests; D012372:ROC Curve; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D012307:Risk Factors; D059467:Transcriptome
chemical_list: D014408:Biomarkers, Tumor; D000074166:Circulating MicroRNA
keywords: diagnosis; exosomes; lung adenocarcinoma; miRNA; plasma