title: Innovative methods to generate clean sugar stream from biomass feedstocks for efficient fermentation.
abstract: Sorghum stalks were evaluated to generate clean sugars; enzymatic hydrolysis efficiency of acid pretreated biomass was significantly low (12.3 g sugar/g pretreated biomass) compared to alkali method (49.1 g sugar/g pretreated biomass). Inhibitory compounds derived from acid pretreatment, such as hydroxymethyl furfural (HMF) and furfural, were effectively removed using an appropriate detoxification method using 1% (w/v) activated charcoal. The resin mixture containing cationic exchangers (DOWEX 50WX8) and anionic exchangers (IRA 743) in 7:3 ratio at pH 2.7 completely removed HMF, acetic acid and formic acid with less than 2% (w/w) sugar loss, leading to efficient detoxification of inhibitory compounds.
authors.affiliation: Bioprocessing and Renewable Energy Laboratory, Grain Science and Industry, Kansas State University, Manhattan, KS, USA.
authors.forename: Jung-Eun
authors.initials: JE
authors.lastname: Lee
authors.affiliation: Bioprocessing and Renewable Energy Laboratory, Grain Science and Industry, Kansas State University, Manhattan, KS, USA.|BioProcess Algae LLC, 1811 Aksarben Drive, Omaha, NE, USA.
authors.forename: Yadhu N
authors.initials: YN
authors.lastname: Guragain
authors.affiliation: Bioprocessing and Renewable Energy Laboratory, Grain Science and Industry, Kansas State University, Manhattan, KS, USA.
authors.forename: Krishna P
authors.initials: KP
authors.lastname: Bastola
authors.affiliation: Bioprocessing and Renewable Energy Laboratory, Grain Science and Industry, Kansas State University, Manhattan, KS, USA. vadlani@k-state.edu.|Department of Chemical Engineering, Kansas State University, Manhattan, KS, USA. vadlani@k-state.edu.
authors.forename: Praveen V
authors.initials: PV
authors.lastname: Vadlani
mesh_terms: D018533:Biomass; D002241:Carbohydrates / Q000737:chemistry*; D006868:Hydrolysis; D018547:Plant Stems / Q000737:chemistry*; D045868:Sorghum / Q000737:chemistry*
chemical_list: D002241:Carbohydrates
keywords: Activated charcoal; Detoxification; Hydrolysates; Inhibitors; Ionic exchangers