title: Horizontal gene transfer versus biostimulation: A strategy for bioremediation in Goa.
abstract: Bioaugmentation, Biostimulation and Horizontal gene transfer (HGT) of catabolic genes have been proven for their role in bioremediation of hydrocarbons. It also has been proved that selection of either biostimulation or bioremediation varies for every contaminated site. The reliability of HGT compared to biostimulation and bioremediation was not tested. The present study focuses on reliability of biostimulatiion, bioaugmentation and HGT during biodegradation of Diesel oil and Non aqueous phase liquids (NAPL). Pseudomonas aeruginosa (AEBBITS1) having alkB and NDO genes was used for bioaugmentation and the experiment was conducted using seawater as medium. Based on Gas chromatography results diesel was found to be degraded to 100% in both presence and absence of AEBBITS1. Denturing gradient gel electrophoresis result showed same pattern in presence and absence of AEBBITS1 indicating no HGT. NAPL degradation was found to be more by Biostimulated Bioaugmentation compared to biostimulation and bioaugmentation alone. This proves that biostimulated bioaugmentation is better strategy for oil contamination (tarabll) in Velsao beach, Goa.
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS-Pilani, K K Birla Goa Campus, India. Electronic address: rajesh.pasumarthi9@gmail.com.
authors.forename: Rajesh
authors.initials: R
authors.lastname: Pasumarthi
authors.affiliation: Applied and Environmental Biotechnology Laboratory, Department of Biological Sciences, BITS-Pilani, K K Birla Goa Campus, India. Electronic address: srikanth@goa.bits-pilani.ac.in.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Mutnuri
mesh_terms: D001673:Biodegradation, Environmental; D005742:Gasoline; D022761:Gene Transfer, Horizontal; D006838:Hydrocarbons; D007194:India; D059392:Petroleum Pollution; D011550:Pseudomonas aeruginosa; D014874:Water Pollutants, Chemical
chemical_list: D005742:Gasoline; D006838:Hydrocarbons; D014874:Water Pollutants, Chemical
keywords: Bioaugmentation; Biostimulation; Horizontal gene transfer; Hydrocarbon degradation