title: Genome-Wide Screen for Haploinsufficient Cell Size Genes in the Opportunistic YeastCandida albicans.
abstract: One of the most critical but still poorly understood aspects of eukaryotic cell proliferation is the basis for commitment to cell division in late G1 phase, called Start in yeast and the Restriction Point in metazoans. In all species, a critical cell size threshold coordinates cell growth with cell division and thereby establishes a homeostatic cell size. While a comprehensive survey of cell size genetic determinism has been performed in the saprophytic yeastsSaccharomyces cerevisiaeandSchizosaccharomyces pombe, very little is known in pathogenic fungi. As a number of critical Start regulators are haploinsufficient for cell size, we applied a quantitative analysis of the size phenome, using elutriation-barcode sequencing methodology, to 5639 barcoded heterozygous deletion strains of the opportunistic yeastCandida albicansOur screen identified conserved known regulators and biological processes required to maintain size homeostasis in the opportunistic yeastC. albicansWe also identified novelC. albicans-specific size genes and provided a conceptual framework for future mechanistic studies. Interestingly, some of the size genes identified were required for fungal pathogenicity suggesting that cell size homeostasis may be elemental toC. albicansfitness or virulence inside the host.
authors.affiliation: Infectious Diseases Research Centre, Centre Hospitalier Universitaire (CHU) de Québec Research Center, Université Laval, Quebec City, Quebec, Canada.
authors.forename: Julien
authors.initials: J
authors.lastname: Chaillot
authors.affiliation: Centre for Systems Biology, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, G1V 4G2 Canada.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Cook
authors.affiliation: Infectious Diseases Research Centre, Centre Hospitalier Universitaire (CHU) de Québec Research Center, Université Laval, Quebec City, Quebec, Canada.|Department of Molecular Medicine, Université Laval, Quebec City, Quebec, Canada.
authors.forename: Jacques
authors.identifier: 0000-0002-9973-2740
authors.initials: J
authors.lastname: Corbeil
authors.affiliation: Infectious Diseases Research Centre, Centre Hospitalier Universitaire (CHU) de Québec Research Center, Université Laval, Quebec City, Quebec, Canada adnane.sellam@crchudequebec.ulaval.ca adnane.sellam@gmail.com.|Department of Microbiology, Infectious Disease and Immunology, Faculty of Medicine, Université Laval, Quebec City, Quebec, G1V 4G2 Canada.
authors.forename: Adnane
authors.identifier: 0000-0002-0506-7752
authors.initials: A
authors.lastname: Sellam
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D002176:Candida albicans / Q000235:genetics* / Q000472:pathogenicity; D002453:Cell Cycle / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics*; D048429:Cell Size; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification; D016681:Genome, Fungal; D057895:Haploinsufficiency / Q000235:genetics*
chemical_list: D005656:Fungal Proteins
keywords: Candida albicans; Start control; cell size; haploinsufficiency