title: Inhibition of Anaphase-Promoting Complex by Silence APC/CCdh1to Enhance Radiosensitivity of Nasopharyngeal Carcinoma Cells.
abstract: The aim of this study was to investigate the possibility of APC/CCdh1as a potential therapeutic target in the radiosensitivity of nasopharyngeal carcinoma (NPC) cell CNE-1, and explain the role of APC subunits after silence of Cdh1 combined with radiotherapy. Transfection with Cdh1 shRNA significantly increased the radiosensitivity of CNE-1 cells and the radiation enhancement ratio (RER) of sh-Cdh1 cells was 1.76. Knockdown of Cdh1 in CNE-1 cells increased irradiation induced apoptosis and G2/M phase cell cycle arrest. The levels of CDC20 and CylinB1 increased and the levels of Ku70 and APC3 decreased after irradiation. APC/CCdh1is involved in regulation of radiosensitivity in human NPC CNE-1 cells. Our study may provide a promising therapeutic strategy for NPC by targeting Cdh1. J. Cell. Biochem. 118: 3150-3157, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors.forename: Chunmiao
authors.initials: C
authors.lastname: Wang
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors.forename: Zhengying
authors.initials: Z
authors.lastname: Su
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors.forename: Huaxin
authors.initials: H
authors.lastname: Hou
authors.affiliation: Department of the Basic Researcher, Guangxi Institute for Cancer Research, Hedi Road No. 71, Nanning 530021, China.
authors.forename: Danrong
authors.initials: D
authors.lastname: Li
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors.forename: Zhiyu
authors.initials: Z
authors.lastname: Pan
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Tian
authors.affiliation: College of Pharmacy, Guangxi Medical University, Nanning 530021, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Mo
mesh_terms: D015703:Antigens, CD; D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome / Q000235:genetics / Q000378:metabolism*; D017209:Apoptosis; D015820:Cadherins / Q000235:genetics / Q000378:metabolism*; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism* / Q000532:radiotherapy; D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006801:Humans; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000532:radiotherapy; D011836:Radiation Tolerance
chemical_list: D015703:Antigens, CD; D064191:Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome; C577366:CDC27 protein, human; C513384:CDH1 protein, human; D015820:Cadherins
keywords: APC/C ACTIVATOR Cdh1; NPC CNE-1 CELLS; RADIOSENSITIVITY; ShRNA; THE ANAPHASE-PROMOTING COMPLEX (APC)