title: Molecular typing and virulence analysis of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from Egyptian hospitals.
abstract: Klebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK efflux pump (32.14%) in the MDR isolates. FimH-1 and mrkD genes were prevalent in wound and blood isolates. FimH-1 gene was prevalent in sputum while mrkD gene was prevalent in urine. Serum resistance associated with outer membrane protein coding gene (traT) was found in all blood isolates. IucC, entB, and Irp-1 were detected in 32.14%, 78.5% and 10.7% of MDR isolates, respectively. We used two Polymerase Chain Reaction (PCR) analyses: Enterobacterial Repetitive Intergenic Consensus (ERIC) and Random Amplified Polymorphic DNA (RAPD). ERIC-PCR revealed 21 and RAPD-PCR revealed 18 distinct patterns of isolates with similarity ≥80%. ERIC genotyping significantly correlated with resistance patterns and virulence determinants. RAPD genotyping significantly correlated with resistance patterns but not with virulence determinants. Both RAPD and ERIC genotyping methods had no correlation with the capsule types. These findings can help up better predict MDR Klebsiella pneumoniae outbreaks associated with specific genotyping patterns.
authors.affiliation: Department of Microbiology &Immunology, Faculty of Pharmacy, October University for Modern Sciences and Arts, Giza, Egypt.
authors.forename: Reham
authors.initials: R
authors.lastname: Wasfi
authors.affiliation: Department of Microbiology &Immunology, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.|Department of Pharmacy Practice, School of Pharmacy, Chapman University, Orange, California, USA.
authors.forename: Walid F
authors.initials: WF
authors.lastname: Elkhatib
authors.affiliation: Department of Biological Sciences, College of Arts and Sciences, University of South Florida St. Petersburg, St. Petersburg, Florida, USA.|Department of Microbiology and Immunology, Faculty of Pharmacy, Cairo University, Cairo, Egypt.
authors.forename: Hossam M
authors.initials: HM
authors.lastname: Ashour
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004534:Egypt; D005260:Female; D060005:Genotyping Techniques; D006761:Hospitals; D006801:Humans; D007710:Klebsiella Infections / Q000235:genetics*; D007711:Klebsiella pneumoniae / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity; D008297:Male
chemical_list: D001426:Bacterial Proteins