title: DNAzymes Dz13 target the c-jun possess antiviral activity against influenza A viruses.
abstract: The emergence of anti-influenza A virus drugs resistant strain highlights the need for more effective therapy. Our earlier study demonstrated that c-jun, a downstream molecule of JNK, might be important in viral infections and inflammatory responses. In the present study, we explored the function of DNAzymes Dz13 that target c-jun in influenza A virus infected mice. Dz13 displayed non-toxic side effects on A549 cells and BALB/c mice. Moreover, Dz13-treated mice had enhanced survival after influenza compared with untreated mice. Simultaneously, the pulmonary inflammatory responses and viral burden were decreased in Dz13 treated mice. Furthermore, proliferation levels of infection-induced CD4+and CD8+T cells were impaired. These data demonstrated that Dz13 could reduce viral replication and inflammatory response in vivo, suggesting that Dz13 may potentially be used to treat influenza A viral infection.
authors.affiliation: Henan Institute of Science and Technology, Xinxiang 453003, China.
authors.forename: Zhaopei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Henan Institute of Science and Technology, Xinxiang 453003, China; Postdoctoral Research Base, Henan Institute of Science and Technology, Xinxiang 453003, China; Post-doctoral Research Station, Henan Agriculture University, Zhengzhou 450002, China.
authors.forename: Shouping
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Henan Institute of Science and Technology, Xinxiang 453003, China. Electronic address: wangsanhu@hist.edu.cn.
authors.forename: Sanhu
authors.initials: S
authors.lastname: Wang
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D016207:Cytokines / Q000096:biosynthesis; D021881:DNA, Catalytic / Q000235:genetics*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D016758:Genes, jun; D006801:Humans; D009980:Influenza A virus / Q000235:genetics / Q000276:immunology; D008213:Lymphocyte Activation / Q000276:immunology; D051379:Mice; D009976:Orthomyxoviridae Infections / Q000235:genetics* / Q000276:immunology / Q000401:mortality / Q000821:virology*; D016176:T-Lymphocyte Subsets / Q000276:immunology / Q000378:metabolism; D014779:Virus Replication
chemical_list: D016207:Cytokines; D021881:DNA, Catalytic; C485475:Dz13 DNAzyme
keywords: Antiviral; C-jun; Dz13; Influenza A virus