title: Adhesion and differentiation of Saos-2 osteoblast-like cells on chromium-doped diamond-like carbon coatings.
abstract: Diamond-like carbon (DLC) thin films are promising for use in coating orthopaedic, dental and cardiovascular implants. The problem of DLC layers lies in their weak layer adhesion to metal implants. Chromium is used as a dopant for improving the adhesion of DLC films. Cr-DLC layers were prepared by a hybrid technology, using a combination of pulsed laser deposition (PLD) from a graphite target and magnetron sputtering. Depending on the deposition conditions, the concentration of Cr in the DLC layers moved from zero to 10.0 at.%. The effect of DLC layers with 0.0, 0.9, 1.8, 7.3, 7.7 and 10.0 at.% Cr content on the adhesion and osteogenic differentiation of human osteoblast-like Saos-2 cells was assessed in vitro. The DLC samples that contained 7.7 and 10.0 at.% of Cr supported cell spreading on day 1 after seeding. On day three after seeding, the most apparent vinculin-containing focal adhesion plaques were also found on samples with higher concentrations of chromium. On the other hand, the expression of type I collagen and alkaline phosphatase at the mRNA and protein level was the highest on Cr-DLC samples with a lower concentration of Cr (0-1.8 at.%). We can conclude that higher concentrations of chromium supported cell adhesion; however DLC and DLC doped with a lower concentration of chromium supported osteogenic cell differentiation.
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Elena
authors.initials: E
authors.lastname: Filova
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic. marta.vandrovcova@fgu.cas.cz.
authors.forename: Marta
authors.initials: M
authors.lastname: Vandrovcova
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21, Prague 8, Czech Republic.|Czech Technical University in Prague, Faculty of Biomedical Engineering, Nam. Sitna 3105, 272 01, Kladno, Czech Republic.
authors.forename: Miroslav
authors.initials: M
authors.lastname: Jelinek
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21, Prague 8, Czech Republic.
authors.forename: Josef
authors.initials: J
authors.lastname: Zemek
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21, Prague 8, Czech Republic.
authors.forename: Jana
authors.initials: J
authors.lastname: Houdkova
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21, Prague 8, Czech Republic.|Czech Technical University in Prague, Faculty of Biomedical Engineering, Nam. Sitna 3105, 272 01, Kladno, Czech Republic.
authors.lastname: Jan Remsa
authors.affiliation: Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, 182 21, Prague 8, Czech Republic.|Czech Technical University in Prague, Faculty of Biomedical Engineering, Nam. Sitna 3105, 272 01, Kladno, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Kocourek
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Lubica
authors.initials: L
authors.lastname: Stankova
authors.affiliation: Department of Biomaterials and Tissue Engineering, Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20, Prague 4, Czech Republic.
authors.forename: Lucie
authors.initials: L
authors.lastname: Bacakova
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D002244:Carbon / Q000737:chemistry*; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D002857:Chromium / Q000737:chemistry*; D020099:Coated Materials, Biocompatible; D024042:Collagen Type I / Q000378:metabolism; D018130:Diamond / Q000737:chemistry; D022001:Focal Adhesions; D020869:Gene Expression Profiling; D006801:Humans; D007834:Lasers; D008670:Metals / Q000737:chemistry; D010006:Osteoblasts / Q000166:cytology*; D010012:Osteogenesis; D012333:RNA, Messenger / Q000378:metabolism; D013499:Surface Properties; D016608:Talin / Q000737:chemistry; D016596:Vinculin / Q000378:metabolism
chemical_list: D020099:Coated Materials, Biocompatible; D024042:Collagen Type I; D008670:Metals; D012333:RNA, Messenger; D016608:Talin; D002857:Chromium; D016596:Vinculin; D002244:Carbon; D018130:Diamond; D000469:Alkaline Phosphatase