title: Clinical Significance of Dynamics of Programmed Death Ligand-1 Expression on Circulating CD14+Monocytes and CD19+B Cells with the Progression of Hepatitis B Virus Infection.
abstract: Programmed death-1 (PD-1) expression has been revealed to be upregulated on T cells and contributes to T cell exhaustion in patients with hepatitis B virus (HBV) infection. In this study, we investigated the dynamic expression of programmed death ligand-1 (PD-L1), the ligand of PD-1, on circulating CD14+monocytes and CD19+B cells of HBV-infected patients at the stages of chronic HBV (CHB) infection, liver cirrhosis (LC), and hepatocellular carcinoma (HCC), respectively. The results showed that compared with healthy controls, the levels of PD-L1 expression on CD14+and CD19+populations were both upregulated in CHB, LC, and HCC groups. Although there was no significant difference of PD-L1 expression on CD14+population among three disease groups, further analysis demonstrated that the frequency of CD14+PD-L1+population was negatively correlated with HBV DNA load, the levels of alanine aminotransaminase (ALT), and the levels of aspartate aminotransferase (AST), respectively, at CHB stage, while it did not present significant correlation with such parameters at LC stage and was only positively correlated with HBV DNA load at HCC stage. Similarly, the levels of PD-L1 expression on CD19+population also did not present much difference among three disease groups. Intriguingly, the frequencies of CD19+PD-L1+population at CHB and LCC stages were both positively correlated with the levels of ALT and AST, but they were not significantly correlated with HBV DNA load. Thereby, the current study elucidated the dynamics of PD-L1 expression on monocytes and B cells, along with the dynamic regulation of PD-1 on T cells, which had a close relationship during the progression of HBV infection. Collectively, our findings demonstrated that in the course of HBV infection development, PD-L1 expression on CD14+monocytes and CD19+B cells varied and significantly correlated with clinical parameters, which could be utilized as a potential clinical indicator.
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .|2 Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University , Suzhou, China .
authors.forename: Zi-Yi
authors.initials: ZY
authors.lastname: Huang
authors.affiliation: 3 The Affiliated Infectious Hospital of Soochow University , Suzhou, China .
authors.forename: Ping
authors.initials: P
authors.lastname: Xu
authors.affiliation: 4 Central Blood Station of Jiaxing , Zhejiang, China .
authors.forename: Jian-Hua
authors.initials: JH
authors.lastname: Li
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .
authors.forename: Chen-Hua
authors.initials: CH
authors.lastname: Zeng
authors.affiliation: 3 The Affiliated Infectious Hospital of Soochow University , Suzhou, China .
authors.forename: Hua-Feng
authors.initials: HF
authors.lastname: Song
authors.affiliation: 3 The Affiliated Infectious Hospital of Soochow University , Suzhou, China .
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .
authors.forename: Yi-Bei
authors.initials: YB
authors.lastname: Zhu
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Song
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .
authors.forename: Han-Lin
authors.initials: HL
authors.lastname: Lu
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .
authors.forename: Chun-Ping
authors.initials: CP
authors.lastname: Shen
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .|2 Jiangsu Institute of Clinical Immunology, The First Affiliated Hospital of Soochow University , Suzhou, China .
authors.forename: Xue-Guang
authors.initials: XG
authors.lastname: Zhang
authors.affiliation: 3 The Affiliated Infectious Hospital of Soochow University , Suzhou, China .
authors.forename: Mei-Ying
authors.initials: MY
authors.lastname: Wu
authors.affiliation: 1 Department of Biochemistry and Molecular Biology, School of Biology and Basic Medical Sciences, Soochow University , Suzhou, China .
authors.forename: Xue-Feng
authors.initials: XF
authors.lastname: Wang
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D018941:Antigens, CD19 / Q000032:analysis; D001219:Aspartate Aminotransferases / Q000097:blood; D001402:B-Lymphocytes / Q000737:chemistry*; D060890:B7-H1 Antigen / Q000032:analysis*; D006528:Carcinoma, Hepatocellular / Q000473:pathology*; D004279:DNA, Viral / Q000097:blood; D005260:Female; D019694:Hepatitis B, Chronic / Q000150:complications / Q000473:pathology*; D006801:Humans; D018950:Lipopolysaccharide Receptors / Q000032:analysis; D008103:Liver Cirrhosis / Q000150:complications / Q000473:pathology*; D008113:Liver Neoplasms / Q000473:pathology*; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000737:chemistry*; D011379:Prognosis; D019562:Viral Load
chemical_list: D018941:Antigens, CD19; D060890:B7-H1 Antigen; C000604811:CD19 molecule, human; C423236:CD274 protein, human; D004279:DNA, Viral; D018950:Lipopolysaccharide Receptors; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase
keywords: B cells; hepatitis B virus infection; monocytes; programmed death ligand-1