title: Mesoporous silica nanoparticles for efficient rivastigmine hydrogen tartrate delivery into SY5Y cells.
abstract: Rivastigmine hydrogen tartrate (RT) is a molecule with both hydrophilic and hydrophobic properties used for the treatment of the Alzheimer's disease. In this work, the larger pore size of mesoporous silica nanoparticles (P1-MSN) was synthesized and then, P1-MSN were functionalized by succinic anhydride (S-P1-MSN) and 3-aminopropyltriethoxysilane (APTES) (AP-CO-P1-MSN) using the grafting and co-condensation methods, respectively. A new method was used for the functionalization of P1-MSN by succinic anhydride at room temperature. Nanoparticles were characterized by special instrumental analysis and loaded by RT. Maximum entrapment efficiency and RT loading percentage into P1-MSN, AP-CO-P1-MSN and S-P1-MSN were respectively obtained as 21.26 and 25.5%, 41.5 and 49.8%, and 11.9 and 14.28% for 24 h. In the simulated gastric and body fluids, the release rate of RT-loaded AP-CO-P1-MSN (AP-CO-P1-MSN-RT) was lower than that of other RT-loaded nanoparticles. In oral pathway, the sustained release of RT was observed in AP-CO-P1-MSN-RT. Moreover, no cytotoxicity effect was observed for P1-MSN, but the cells treated by AP-CO-P1-MSN showed a reduction in SY5Y cell viability due to easy entrance of these nanoparticles and their accumulation in different parts of the cell as observed by TEM.
authors.affiliation: a Chemical Engineering Department , Payam Noor University , Tehran , Iran.
authors.forename: Mahmonir
authors.initials: M
authors.lastname: Karimzadeh
authors.affiliation: b Faculty of Food Industry and Agriculture , Standard Research Institute, Iranian National Standards Organization , Karaj , Iran.
authors.forename: Ladan
authors.initials: L
authors.lastname: Rashidi
authors.affiliation: c Biotechnology Division, Faculty of Chemical Engineering , Tarbiat Modares University , Tehran , Iran.
authors.forename: Fariba
authors.initials: F
authors.lastname: Ganji
mesh_terms: D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D003692:Delayed-Action Preparations / Q000008:administration & dosage / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D016062:Porosity; D011437:Propylamines / Q000737:chemistry; D000068836:Rivastigmine / Q000008:administration & dosage* / Q000737:chemistry*; D012821:Silanes / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*; D013387:Succinic Anhydrides / Q000737:chemistry
chemical_list: D003692:Delayed-Action Preparations; D011437:Propylamines; D012821:Silanes; D013387:Succinic Anhydrides; C031801:succinic anhydride; D012822:Silicon Dioxide; C477625:amino-propyl-triethoxysilane; D000068836:Rivastigmine
keywords: Alzheimer; Rivastigmine hydrogen tartrate; cytotoxicity; mesoporous silica; nanoparticles