title: Studying the Mechanism of Membrane Permeabilization Induced by Antimicrobial Peptides Using Patch-Clamp Techniques.
abstract: Many short peptides selectively permeabilize the bacteria plasma membrane, leading to their lyses and death: they are therefore a source of antibacterial molecules and inspiration for novel and more selective drugs, which may have wider application in many other fields, as selective anticancer drugs. In this chapter, it is presented a new method to investigate the permeabilization properties of antimicrobial peptides under strict physiological conditions, employing the patch-clamp technique coupled to a fast perfusion system.
authors.affiliation: Department of Life Science and Biotechnology, University of Ferrara, Via Ludovico Ariosto 35, Ferrara, 4421, Italy. rsg@unife.it.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Rispoli
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000494:pharmacology*; D016466:CHO Cells; D002462:Cell Membrane / Q000187:drug effects* / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects*; D003412:Cricetulus; D055724:Electrophysiological Phenomena / Q000187:drug effects; D008564:Membrane Potentials / Q000187:drug effects; D018408:Patch-Clamp Techniques / Q000295:instrumentation / Q000379:methods
chemical_list: D023181:Antimicrobial Cationic Peptides
keywords: CHO-K1 cells; Ion channels; Photoreceptors; Pore-forming peptides; Pore-forming toxins; Rod outer segment; Whole-cell recording