title: Expressions of adenosine A2Areceptors in coronary arteries and peripheral blood mononuclear cells are correlated in coronary artery disease patients.
abstract: BACKGROUND
  Altered coronary blood flow occurs in patients with coronary artery disease (CAD). Adenosine strongly impacts blood flow mostly via adenosine A2Areceptor (A2AR) expressed in coronary tissues. As part of a systemic regulation of the adenosinergic system, we compared A2AR expression in situ, and on peripheral blood mononuclear cells (PBMC) in CAD patients.
  
  
  RESULTS
  Aortic and coronary tissues, and PBMC were sampled in 20 CAD patients undergoing coronary artery bypass surgery and consecutively included. Controls were PBMC obtained from 15 healthy subjects. Expression and activity of A2AR were studied by Western blotting and cAMP measurement, respectively. A2AR expression on PBMC was lower in patients than in controls (0.83±0.31 vs 1.2±0.35 arbitrary units; p<0.01), and correlated with A2AR expression in coronary and aortic tissues (Pearson's r: 0.77 and 0.59, p<0.01, respectively). Basal and maximal cAMP productions following agonist stimulation of PBMC were significantly lower in patients than in controls (120±42 vs 191±65 and 360±113 vs 560±215pg/106cells, p<0.05, respectively). In CAD patients, the increase from basal to maximal cAMP production in PBMC and aortic tissues was similar (+300% and +246%, respectively).
  
  
  CONCLUSIONS
  Expression of A2AR on PBMC correlated with those measured in coronary artery and aortic tissues in CAD patients, A2AR activity of PBMC matched that observed in aorta, and A2AR expression and activity in PBMC were found reduced as compared to controls. Measuring the expression level of A2AR on PBMC represents a good tool to address in situ expression in coronary tissues of CAD patients.
authors.affiliation: Department of Cardiac Surgery, Timone University Hospital, Marseille, France.
authors.forename: Vlad
authors.initials: V
authors.lastname: Gariboldi
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France.
authors.forename: Donato
authors.initials: D
authors.lastname: Vairo
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France; Laboratory of Biochemistry, Timone University Hospital, Marseille, France. Electronic address: guieu.regis@orange.fr.
authors.forename: Régis
authors.initials: R
authors.lastname: Guieu
authors.affiliation: Laboratory of Biochemistry, Timone University Hospital, Marseille, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Marlingue
authors.affiliation: Department of Cardiology, Timone University Hospital, Marseille, France.
authors.forename: Eléonore
authors.initials: E
authors.lastname: Ravis
authors.affiliation: Department of Anesthesia, Timone University Hospital, Marseille, France.
authors.forename: David
authors.initials: D
authors.lastname: Lagier
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France.
authors.forename: Alissa
authors.initials: A
authors.lastname: Mari
authors.affiliation: Laboratory of Biochemistry, Timone University Hospital, Marseille, France.
authors.forename: Elsa
authors.initials: E
authors.lastname: Thery
authors.affiliation: Department of Cardiac Surgery, Timone University Hospital, Marseille, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Collart
authors.affiliation: Department of Vascular Surgery, Timone University Hospital, Marseille, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Gaudry
authors.affiliation: Department of Cardiology, North University Hospital, Marseille, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Bonello
authors.affiliation: Department of Cardiology, North University Hospital, Marseille, France.
authors.forename: Franck
authors.initials: F
authors.lastname: Paganelli
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France.
authors.forename: Jocelyne
authors.initials: J
authors.lastname: Condo
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Kipson
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France.
authors.forename: Emmanuel
authors.initials: E
authors.lastname: Fenouillet
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Ruf
authors.affiliation: UMR MD2, Aix-Marseille University and IRBA, Marseille, France; Laboratory of Biochemistry, Timone University Hospital, Marseille, France.
authors.forename: Giovanna
authors.initials: G
authors.lastname: Mottola
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D001011:Aorta / Q000378:metabolism; D016022:Case-Control Studies; D001026:Coronary Artery Bypass; D003324:Coronary Artery Disease / Q000378:metabolism* / Q000473:pathology / Q000601:surgery; D003331:Coronary Vessels / Q000378:metabolism; D005260:Female; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D043705:Receptor, Adenosine A2A / Q000378:metabolism*
chemical_list: D043705:Receptor, Adenosine A2A
keywords: Adenosine A(2A) receptor; Coronary artery disease; Ischemia