title: Proteomic analysis of irradiated lung tissue of mice using gel-based proteomic approach.
abstract: Exposure to radiation causes severe alterations of protein expression level inside the cell, thus it may influence the biological events and stress response. In the present investigation, we have demonstrated the effect of radiation on mice lung tissues.
  
  
  
  Two-dimensional gel electrophoresis (2-DE) coupled with MALDI-TOF/TOF was used to check the expression changes in lung proteome profile of strain 'A' female mice after exposure to lethal doses of gamma irradiation at different time periods (24 and 48 h). Identified proteins were analysed for their altered expression and were further validated by Western blotting and enzyme-linked immunosorbent assay (ELISA).
  
  
  
  Nine significant differentially expressed proteins were identified from irradiated lungs tissues. The expression level of zinc finger protein was found to be up regulated at 24 h irradiation in comparison to 48 h irradiation.
  
  
  
  Zinc finger protein may be considered as a radiation responsive protein. Alteration in its expression pattern may primarily affect binding specificity of the protein that can further result in the interference in transcriptional control of multiple stress responsive genes.
authors.affiliation: a Department of Genomics & Molecular Medicine , CSIR - Institute of Genomics & Integrative Biology (IGIB), Delhi University Campus , Delhi , India.
authors.forename: Shabir
authors.initials: S
authors.lastname: Hussain
authors.affiliation: b Division of Radioprotective Drug Development Research , Institute of Nuclear Medicine and Allied Sciences , Delhi , India.
authors.forename: Ajaswrata
authors.initials: A
authors.lastname: Dutta
authors.affiliation: a Department of Genomics & Molecular Medicine , CSIR - Institute of Genomics & Integrative Biology (IGIB), Delhi University Campus , Delhi , India.
authors.forename: Ashish
authors.initials: A
authors.lastname: Sarkar
authors.affiliation: b Division of Radioprotective Drug Development Research , Institute of Nuclear Medicine and Allied Sciences , Delhi , India.
authors.forename: Abhinav
authors.initials: A
authors.lastname: Singh
authors.affiliation: b Division of Radioprotective Drug Development Research , Institute of Nuclear Medicine and Allied Sciences , Delhi , India.
authors.forename: Manju Lata
authors.initials: ML
authors.lastname: Gupta
authors.affiliation: a Department of Genomics & Molecular Medicine , CSIR - Institute of Genomics & Integrative Biology (IGIB), Delhi University Campus , Delhi , India.
authors.forename: Sagarika
authors.initials: S
authors.lastname: Biswas
mesh_terms: D000818:Animals; D004307:Dose-Response Relationship, Radiation; D005260:Female; D005720:Gamma Rays; D020869:Gene Expression Profiling / Q000379:methods*; D008168:Lung / Q000378:metabolism* / Q000528:radiation effects*; D051379:Mice; D020543:Proteome / Q000378:metabolism*; D011829:Radiation Dosage; D011836:Radiation Tolerance / Q000502:physiology; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods*; D057825:Two-Dimensional Difference Gel Electrophoresis / Q000379:methods*
chemical_list: D020543:Proteome
keywords: 2-DE; Ionizing radiation; MALDI-TOF; lung proteome; zinc finger protein