title: Microwave-Assisted Synthesis of Antimicrobial Peptides.
abstract: Antimicrobial peptides (AMPs) are emerging as one of the unsurpassed therapeutic tools to treat various devastating diseases that are affecting millions of lives. Conventional synthesis of peptides requires longer times, and hence automated microwave technology could be regarded as an alternative implement which offers advantages like less reaction times and higher yields. In this sense, we herein describe a methodology to prepare AMPs through solid-phase peptide synthesis under microwave conditions. We have used LL37 as an example to discuss the synthetic protocol including the difficulties involved in the preparation of so-called long and difficult peptides and also remedial procedures to overcome these obstacles.
authors.affiliation: Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
authors.forename: Suhas
authors.initials: S
authors.lastname: Ramesh
authors.affiliation: Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
authors.forename: Beatriz G
authors.initials: BG
authors.lastname: de la Torre
authors.affiliation: Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.|School of Chemistry and Physics, University of KwaZulu-Natal, Durban, South Africa.|CIBER-BBN, Networking Centre on Bioengineering, Biomaterials and Nanomedicine, Barcelona Science Park, Barcelona, Spain.|Department of Organic Chemistry, University of Barcelona, Barcelona, Spain.
authors.forename: Fernando
authors.initials: F
authors.lastname: Albericio
authors.affiliation: Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Durban, South Africa.
authors.forename: Hendrik G
authors.initials: HG
authors.lastname: Kruger
authors.affiliation: Catalysis and Peptide Research Unit, School of Health Sciences, University of KwaZulu-Natal, Westville Campus, E-Block, 6th Floor, Room E1-06-016, Durban, 4001, South Africa. govenderthav@icloud.com.
authors.forename: Thavendran
authors.initials: T
authors.lastname: Govender
mesh_terms: D000890:Anti-Infective Agents / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D023181:Antimicrobial Cationic Peptides / Q000138:chemical synthesis* / Q000737:chemistry / Q000302:isolation & purification; D054804:Cathelicidins / Q000138:chemical synthesis / Q000737:chemistry; D060326:Chemistry Techniques, Synthetic; D006801:Humans; D008872:Microwaves
chemical_list: D000890:Anti-Infective Agents; D023181:Antimicrobial Cationic Peptides; D054804:Cathelicidins
keywords: Antimicrobial peptides; Automated; LL37; Microwave; SPPS