title: In vitroevaluation of aliphatic fatty alcohol metabolites ofPerseaamericanaseed as potential antimalarial and antimicrobial agents.
abstract: PerseaamericanaMill (Lauraceae) is a local medicinal plant used in Nigerian ethnomedicine as antimalarial. The aqueous decoction of the root part is a potent remedy against bacterial infections. Hence, the need to investigate the phytochemical and biological activities (antimicrobial and antiplasmodial) of the root back ofPerseaamericana. Chromatographic and spectroscopic techniques were used in the identification and purification of metabolites, which were assayed for antimalarial and antimicrobial activities using Plasmodium falciparum and a panel of microorganisms. From the seeds ofP. americana, five known 1, 2, 4-dihydroxy derivatives aliphatic alcohols, called avocadenols were isolated and identified by spectroscopic methods including 1D- and 2D NMR, and comparison with reported data in literature. Antifungal activity for 1, 2, 4-Trihydroxyheptadec-6-en-16-yne (5) (IC50< 8 μg/mL) against all the fungal strains andS. areus,and antimalarial activity for compounds 1, 2, 4-Trihydroxyheptadec-16-ene (1) and 1, 2, 4-tetrahydroxyheptadecane-6, 16-diene(2) (IC50= 1.6 and 1.4 μg/mL for the D6 clone, respectively, and 2.1 and 1.4 μg/mL for the W2 clone, respectively) was observed. The fatty alcohols 1, 2, 4-tetrahydroxyheptadecane-6, 16-diene(2); 1, 2, 4-Trihydroxyheptadec-16-yne(3) and 1, 2, 4-Trihydroxyheptadecane(4) also exhibited promisingin vitroantibacterial activity against a panel of pathogenic bacteriaS. areus,methicillin resistant S. areusandE. coliat IC50values of 21.1, 8, 200 μg/mL), (3.259, 86.32 μg/mL) and (17.18, 8.26 and 200 μg/mL), respectively. The results of this study provide evidence that the fatty alcohols are a promising class of antimalarial and antimicrobial agents.
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Nigeria; Department of Pharmacognosy, School of Pharmacy, University, MS 38655, USA.
authors.forename: A
authors.initials: A
authors.lastname: Falodun
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Nigeria; Institute of Organic Chemistry, University of Restock, 18509, Rostock, Germany.
authors.forename: O
authors.initials: O
authors.lastname: Erharuyi
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Nigeria; Institute of Organic Chemistry, University of Restock, 18509, Rostock, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Imieje
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.
authors.forename: J
authors.initials: J
authors.lastname: Ahomafor
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, University of Benin, Benin City, Nigeria.
authors.forename: C
authors.initials: C
authors.lastname: Akunyuli
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, University, MS 38655, USA.
authors.forename: M
authors.initials: M
authors.lastname: Jacobs
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, University, MS 38655, USA.
authors.forename: S
authors.initials: S
authors.lastname: Khan
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, University, MS 38655, USA.
authors.forename: M T
authors.initials: MT
authors.lastname: Hamann
authors.affiliation: Institute of Organic Chemistry, University of Restock, 18509, Rostock, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Langer
keywords: Perseaamericana; Plasmodium falciparum; antimalarial; antimicrobial isolation; metabolites; seeds