title: Comparison between pediatric and adult adipose mesenchymal stromal cells.
abstract: Adipose-derived mesenchymalstromal cells (ASC) are currently tested in regenerative medicine to promote tissue reconstruction after injury. Regardingautologous purpose, the possible loss of therapeutic function and cell properties during aging have been questioned in adults. To date no reliable information is available concerning ASC from pediatric patients and a better knowledge is required for clinical applications.
  
  
  
  Subcutaneous adipose tissue was collected from 27 donors (0-1 years old) and 50 donors (1-12 years old) and compared with adult ASC for in vitro characteristics. ASC were then tested in a mouse model of limb ischemia.
  
  
  
  Cells from the stromal vascular fraction (SVF) and subsequent cultured ASC were prepared. Only a greater amount in SVF cell number and ASC proliferative rate were found. Cell phenotype, colony formingunit-fibroblast (CFU-F) content, immunomodulation effect and adipogenic, osteoblastic and angiogenic potentials were not significantly different. In vivo, pediatric ASC induced an increase in microangiographic score in a mouse model of limb ischemia, even though improvement in vascular density was not significantly correlated to limb rescue. Finally messengerRNA (mRNA) analysis using a microarray approach identified that only 305 genes were differentially expressed (217 down- and 88 up-regulated) in pediatric versus adult ASC, confirming that ASC from both age groups shared very close intrinsic properties.
  
  
  
  This is the first study reporting a comparative analysis of ASC from a large number of donors and showing that their in vitro and in vivo properties were similar and maintained during aging.
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France; Service de Chirurgie Pédiatrique, Hôpital des Enfants de Toulouse, Toulouse, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Abbo
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France.
authors.forename: Marion
authors.initials: M
authors.lastname: Taurand
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France; Toulouse Faculty of Dentistry, Paul Sabatier University (UPS) and Toulouse University Hospital, Toulouse, France.
authors.forename: Paul
authors.initials: P
authors.lastname: Monsarrat
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France; University Paul Sabatier of Toulouse (UPS), Institut National Polytechnique - Ecole Nationale Vétérinaire de Toulouse (INP-ENVT), Plateforme d'Histopathologie Expérimentale et Comparée, Toulouse, France.
authors.forename: Isabelle
authors.initials: I
authors.lastname: Raymond
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France.
authors.forename: Emmanuelle
authors.initials: E
authors.lastname: Arnaud
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France.
authors.forename: Sandra
authors.initials: S
authors.lastname: De Barros
authors.affiliation: Toulouse Faculty of Dentistry, Paul Sabatier University (UPS) and Toulouse University Hospital, Toulouse, France.
authors.forename: Françoise
authors.initials: F
authors.lastname: Auriol
authors.affiliation: Toulouse Faculty of Dentistry, Paul Sabatier University (UPS) and Toulouse University Hospital, Toulouse, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Galinier
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France.
authors.forename: Louis
authors.initials: L
authors.lastname: Casteilla
authors.affiliation: STROMALab, Université de Toulouse, Toulouse, France. Electronic address: valerie.planat@inserm.fr.
authors.forename: Valerie
authors.initials: V
authors.lastname: Planat-Benard
mesh_terms: D000328:Adult; D000367:Age Factors; D000375:Aging / Q000502:physiology*; D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D002648:Child; D002675:Child, Preschool; D005121:Extremities; D005260:Female; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D007511:Ischemia / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D008297:Male; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D050151:Subcutaneous Fat / Q000166:cytology* / Q000378:metabolism; D055815:Young Adult
keywords: adipose mesenchymalstromal cells; cell therapy; pediatric cells