title: Enrichment of serum biomarkers by magnetic metal-organic framework composites.
abstract: Highly efficient extraction of peptides from serum is critical for finding serum biomarkers using mass spectrometry, which still remains a great challenge. Currently, a bottom-up proteomics approach has been applied to discover serum biomarkers. However, the approach was labor intensive, time and cost consuming, and cannot meet the requirements for clinical application. In this work, Fe3O4/C@MIL-100 composites were synthesized to efficiently capture peptides from microwave-assisted formic acid digests of BSA and human serum prior to MALDI-TOF MS analysis. Fe3O4/C@MIL-100 composites exhibited size-selective adsorption performance, thus providing a rapid and convenient approach to enrich low-abundance peptides. Notably, the peptides' mass fingerprinting of serum digestions between type 2 diabetes mellitus (T2DM) and healthy persons were distinguishable, which indicated the potential ability of this technique for T2DM diagnosis and rapid biomarker discovery. Graphical Abstract Efficient extraction and identification of serum biomarkers using Fe3O4/C@MIL-100 composites from acid hydrolysate.
authors.affiliation: Tianjin Modern Vocational Technology College, Tianjin, 300350, China.
authors.forename: Ji-Ping
authors.initials: JP
authors.lastname: Wei
authors.affiliation: Nankai Hospital of Tianjin City, Tianjin, 300100, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Wang
authors.affiliation: School of Computer Science and Technology, Tianjin Key Laboratory of Cognitive Computing and Application, Tianjin University, Tianjin, 300072, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China.
authors.forename: Zhi-Jiang
authors.initials: ZJ
authors.lastname: Zhou
authors.affiliation: College of Environmental and Chemical Engineering Sciences, Tianjin Polytechnic University, Tianjin, 300387, China. huangyanfeng@tjpu.edu.cn.
authors.forename: Yan-Feng
authors.initials: YF
authors.lastname: Huang
authors.affiliation: School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China. jobin@tju.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Qiao
mesh_terms: D015415:Biomarkers / Q000097:blood*; D003924:Diabetes Mellitus, Type 2 / Q000097:blood; D052203:Ferrosoferric Oxide / Q000737:chemistry*; D006801:Humans; D008280:Magnetics; D008855:Microscopy, Electron, Scanning; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
chemical_list: D015415:Biomarkers; D052203:Ferrosoferric Oxide
keywords: Enrichment; MALDI-TOF MS; Magnetic metal-organic framework composites; Serum biomarker