title: Echinococcus granulosus: Evidence of a heterodimeric glutathione transferase built up by phylogenetically distant subunits.
abstract: In the cestode parasite Echinococcus granulosus, three phylogenetically distant cytosolic glutathione transferases (GSTs) (EgGST1, 2 and 3) were identified. Interestingly, the C-terminal domains of EgGST3 and EgGST2 but not EgGST1, exhibit all amino acids involved in Sigma-class GST dimerization. Here, we provide evidence indicating that EgGST2 and EgGST3 naturally form a heterodimeric structure (EgGST2-3), and also we report the enzymatic activity of the recombinant heterodimer. EgGST2-3 might display novel properties able to influence the infection establishment. This is the first report of a stable heterodimeric GST built up by phylogenetically distant subunits.
authors.affiliation: Cátedra de Inmunología, Facultad de Química, UdelaR, Av. Alfredo Navarro 3051, piso 2, Montevideo, CP 11600, Uruguay.
authors.forename: Paula
authors.initials: P
authors.lastname: Arbildi
authors.affiliation: Cátedra de Inmunología, Facultad de Química, UdelaR, Av. Alfredo Navarro 3051, piso 2, Montevideo, CP 11600, Uruguay.
authors.forename: Silvana
authors.initials: S
authors.lastname: La-Rocca
authors.affiliation: Cátedra de Inmunología, Facultad de Química, UdelaR, Av. Alfredo Navarro 3051, piso 2, Montevideo, CP 11600, Uruguay.
authors.forename: Veronica
authors.initials: V
authors.lastname: Lopez
authors.affiliation: Cátedra de Inmunología, Facultad de Química, UdelaR, Av. Alfredo Navarro 3051, piso 2, Montevideo, CP 11600, Uruguay.
authors.forename: Natalia
authors.initials: N
authors.lastname: Da-Costa
authors.affiliation: Cátedra de Inmunología, Facultad de Química, UdelaR, Av. Alfredo Navarro 3051, piso 2, Montevideo, CP 11600, Uruguay. Electronic address: vfernan@fq.edu.uy.
authors.forename: Veronica
authors.initials: V
authors.lastname: Fernandez
mesh_terms: D000818:Animals; D048209:Echinococcus granulosus / Q000145:classification / Q000201:enzymology* / Q000235:genetics*; D004789:Enzyme Activation; D019143:Evolution, Molecular; D005982:Glutathione Transferase / Q000737:chemistry / Q000235:genetics* / Q000302:isolation & purification / Q000378:metabolism*; D047468:Immunoprecipitation; D010802:Phylogeny; D055503:Protein Multimerization; D021122:Protein Subunits / Q000235:genetics*
chemical_list: D021122:Protein Subunits; D005982:Glutathione Transferase
keywords: Echinococcus granulosus; Enzyme activity; Glutathione transferase; Heterodimer