title: Expression of type III collagen correlates with poor prognosis in oral squamous cell carcinoma.
abstract: OBJECTIVE
  The aim of the present study was to evaluate, compare, and correlate the types of collagen fibers seen in different grades of oral cancer.
  
  
  METHODS
  Thirty cases of histologically-diagnosed, well-, moderately-, and poorly-differentiated oral squamous cell carcinoma (OSCC) were retrieved from the archives of the Institute. Collagen was evaluated using picrosirius red stain and immunohistochemical analysis of the antibody to type III collagen. A correlation between these findings and the grade of OSCC was evaluated.
  
  
  RESULTS
  Collagen fibers showed a change in birefringence ranging from reddish-orange to greenish-yellow in well- to poorly-differentiated oral squamous cell carcinoma. The findings were statistically significant for polarizing colors observed in grades of OSCC (P<.001). Immunohistochemical staining intensity of type III collagen changed from weak to strong as grade increased for OSCC, and was also statistically significant (P<.001).
  
  
  CONCLUSIONS
  In the present study, tumor progression reflected a change in collagen present, from type I to type III. Determination of the type of collagen in different grades of OSCC can facilitate therapeutic targeting of molecules responsible for invasion and progression of oral cancer.
authors.affiliation: Department of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.
authors.forename: Karuna
authors.initials: K
authors.lastname: Kumari
authors.affiliation: Department of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.
authors.forename: Snehashish
authors.initials: S
authors.lastname: Ghosh
authors.affiliation: Department of Maxillofacial Surgery and Diagnostic Sciences, College of Dentistry, Jazan University, Jazan, Saudi Arabia.
authors.forename: Shankargouda
authors.initials: S
authors.lastname: Patil
authors.affiliation: Department of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.
authors.forename: Dominic
authors.initials: D
authors.lastname: Augustine
authors.affiliation: Department of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.
authors.forename: Sowmya
authors.initials: S
authors.lastname: Samudrala Venkatesiah
authors.affiliation: Department of Oral and Maxillofacial Pathology, Faculty of Dental Sciences, Ramaiah University of Applied Sciences, Bangalore, Karnataka, India.
authors.forename: Roopa S
authors.initials: RS
authors.lastname: Rao
mesh_terms: D002294:Carcinoma, Squamous Cell / Q000378:metabolism* / Q000473:pathology; D024061:Collagen Type III / Q000096:biosynthesis*; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D060787:Neoplasm Grading; D011379:Prognosis
chemical_list: D024061:Collagen Type III
keywords: collagen; oral squamous cell carcinoma; picrosirius red