title: Pinoresinol Diglucoside Alleviates oxLDL-Induced Dysfunction in Human Umbilical Vein Endothelial Cells.
abstract: Atherosclerotic cardiovascular diseases are the leading causes of morbidity and mortality worldwide. Deposition of oxidized low-density lipoprotein (oxLDL) is one of the initiators and promoters of atherosclerosis.Eucommialignans were shown to possess antihypertensive effects. This study aimed to investigate the effects of pinoresinol diglucoside (PD), aEucommialignan, on oxLDL-induced endothelial dysfunction. HUVECs were treated with oxLDL and/or PD followed by assessing radical oxygen species (ROS), apoptosis, nitrogen oxide (NO), malondialdehyde (MDA), and superoxide dismutase (SOD) activity with specific assays kits, mRNA levels with quantitative real-time polymerase chain reaction (PCR), and protein levels with western blot. PD abolished oxLDL-induced ROS and MDA production, apoptosis, upregulation of lectin-like oxidized LDL recptor-1 (LOX-1), intercellular Adhesion Molecule 1 (ICAM-1), and nuclear factor kappa-light-chain-enhancer of activated B-cells (NF-κB), and activation of p38MAPK (mitogen-activated protein kinases)/NF-κB signaling. Meanwhile, PD alleviated oxLDL-caused inhibition of SOD activity, eNOS expression, and NO production. These data demonstrated that PD was effective in protecting endothelial cells from oxLDL-caused injuries, which guarantees further investigation on the clinical benefits of PD on cardiovascular diseases.
authors.affiliation: Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China; Department of Cardiology, Yantai Yeda Hospital of Binzhou Medical University, Yantai, Shandong 264100, China.
authors.forename: Jinpeng
authors.identifier: 0000-0001-6359-7375
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Cardiology, Yantai Yeda Hospital of Binzhou Medical University, Yantai, Shandong 264100, China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Zou
authors.affiliation: Department of Geriatrics, Yantai Yeda Hospital of Binzhou Medical University, Yantai, Shandong 264100, China.
authors.forename: Xiangfen
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Qilu Hospital of Shandong University, Jinan, Shandong 250012, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ji
authors.affiliation: Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong 264000, China.
authors.forename: Jun
authors.identifier: 0000-0001-7519-8187
authors.initials: J
authors.lastname: Yang