title: Development of a UPLC-TQ/MS Approach for the Determination of Eleven Bioactive Components in Haizao Yuhu Decoction Plus-Minus Haizao and Gancao Drug Combination after Oral Administration in a Rat Model of Hypothyroidism.
abstract: Haizao Yuhu Decoction (HYD) has been used for approximately 500 years and is well-known in Traditional Chinese Medicine for its efficacy in the treatment of thyroid-related diseases. In this study, a rapid liquid chromatography-tandem mass spectrometry method was developed for the determination of liquiritin, naringin, hesperidin, peimine, liquiritigenin, glycyrrhizic acid, bergapten, nobiletin, osthole, and glycyrrhetinic acid in rat plasma to investigate the pharmacokinetic profile of different HYD prescriptions in a rat model of hypothyroidism. The differences in pharmacokinetic parameters among the groups were compared by Student'st-test. The pharmacokinetic profile of liquiritin, naringin, hesperidin, peimine, liquiritigenin, glycyrrhizic acid, bergapten, nobiletin, osthole, and glycyrrhetinic acid showed significant differences between Haizao and Gancao anti-drug combination and other herbs in HYD. These results may contribute to the rational clinical use of HYD and reveal the compatibility profile of the Haizao and Gancao anti-drug combination.
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. kittyma128@sohu.com.
authors.forename: Yingchang
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. zy91923@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. zhaiyanjuan1990@126.com.
authors.forename: Yuanjuan
authors.initials: Y
authors.lastname: Zhai
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. 04040416@163.com.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. ptianxie@126.com.
authors.forename: Ying
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. qiandwnj@126.com.
authors.forename: Dawei
authors.initials: D
authors.lastname: Qian
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. sushulan1974@163.com.
authors.forename: Shulan
authors.initials: S
authors.lastname: Su
authors.affiliation: Basic Medical College, Nanjing University of Chinese Medicine, Nanjing 210023, China. fanxsh126@126.com.
authors.forename: Xinsheng
authors.initials: X
authors.lastname: Fan
authors.affiliation: Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, and National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. dja@njutcm.edu.cn.
authors.forename: Jinao
authors.initials: J
authors.lastname: Duan
mesh_terms: D000284:Administration, Oral; D000818:Animals; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D003374:Coumarins / Q000097:blood / Q000493:pharmacokinetics*; D004195:Disease Models, Animal; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics* / Q000494:pharmacology; D044950:Flavanones / Q000097:blood / Q000493:pharmacokinetics* / Q000494:pharmacology; D006801:Humans; D007037:Hypothyroidism / Q000188:drug therapy* / Q000276:immunology / Q000473:pathology; D007155:Immunologic Factors / Q000097:blood / Q000493:pharmacokinetics*; D057230:Limit of Detection; D008297:Male; D008516:Medicine, Chinese Traditional; D053978:Pentacyclic Triterpenes / Q000097:blood / Q000493:pharmacokinetics*; D051381:Rats; D017208:Rats, Wistar; D015203:Reproducibility of Results; D013961:Thyroid Gland / Q000187:drug effects / Q000276:immunology / Q000473:pathology
chemical_list: D003374:Coumarins; D004365:Drugs, Chinese Herbal; D044950:Flavanones; D007155:Immunologic Factors; D053978:Pentacyclic Triterpenes; C560811:haizao yuhu
keywords: Haizao Yuhu Decoction; UPLC-TQ/MS; hypothyroidism; multiple components; pharmacokinetic differences