title: SiO2/TiO2Nanocomposite Films on Polystyrene for Light-Induced Cell Detachment Application.
abstract: Light-induced cell detachment shows much potential in in vitro cell culture and calls for high-performance light-responsive films. In this study, a smooth and dense SiO2/TiO2nanocomposite thin film with thickness of around 250 nm was first fabricated on H2O2treated polystyrene (PS) substrate via a low-temperature sol-gel method. It was observed that the film could well-adhere on the PS surface and the bonding strength became increasingly high with the increase of SiO2content. The peeling strength and shear strength reached 3.05 and 30.02 MPa, respectively. It was observed the surface of the film could transform into superhydrophilic upon 20 min illumination of ultraviolet with a wavelength of 365 nm (UV365). In cell culture, cells, i.e., NIH3T3 and MC3T3-E1 cells, cultured on SiO2/TiO2nanocomposite film were easily detached after 10 min of UV365 illumination; the detachment rates reached 90.8% and 88.6%, respectively. Correspondingly, continuous cell sheets with good viability were also easily obtained through the same way. The present work shows that SiO2/TiO2nanocomposite thin film could be easily prepared on polymeric surface at low temperature. The corresponding film exhibits excellent biocompatibility, high bonding strength, and good light responses. It could be a good candidate for the surface of cell culture utensils with light-induced cell detachment property.
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center of Sensor Materials and Applications, Zhejiang University , Hangzhou 310027, China.
authors.forename: Zhiguo
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center of Sensor Materials and Applications, Zhejiang University , Hangzhou 310027, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Cheng
authors.affiliation: School of Materials Science and Engineering, State Key Laboratory of Silicon Materials, Cyrus Tang Center of Sensor Materials and Applications, Zhejiang University , Hangzhou 310027, China.
authors.forename: Wenjian
authors.identifier: http://orcid.org/0000-0002-9373-7284
authors.initials: W
authors.lastname: Weng
mesh_terms: D000818:Animals; D002460:Cell Line; D006861:Hydrogen Peroxide; D051379:Mice; D041681:NIH 3T3 Cells; D053761:Nanocomposites; D011137:Polystyrenes; D012822:Silicon Dioxide; D014025:Titanium
chemical_list: D011137:Polystyrenes; C009495:titanium dioxide; D012822:Silicon Dioxide; D006861:Hydrogen Peroxide; D014025:Titanium
keywords: SiO2/TiO2; cell detachment; light induced; nanocomposite films; polystyrene