title: Phospholipid flippases DnfA and DnfB exhibit differential dynamics within the A. nidulans Spitzenkörper.
abstract: The Spitzenkörper is a structure at the apex of growing cells in many filamentous fungi. Ultrastructural studies indicate that the Spitzenkörper is an organized mass of secretory vesicles, with different types of vesicles present in outer and inner layers. Here, we used live-cell imaging to demonstrate that the phospholipid flippases DnfA and DnfB, which preferentially localize to the outer and inner layers, respectively, exhibit different dynamics in the Spitzenkörper of Aspergillus nidulans. Additionally, deletion of dnfA partially destabilized the Spitzenkörper, while the depletion of cdc50, an essential β-subunit of most flippases, had dramatic effects on hyphal tip organization and morphology.
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77845, USA.
authors.forename: Zachary
authors.initials: Z
authors.lastname: Schultzhaus
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Wenhui
authors.initials: W
authors.lastname: Zheng
authors.affiliation: State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Zonghua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Centro de Investigación Científica y de Educación Superior de Ensenada, Ensenada, Baja California, Mexico.
authors.forename: Rosa
authors.initials: R
authors.lastname: Mouriño-Pérez
authors.affiliation: Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX 77845, USA. Electronic address: bdshaw@tamu.edu.
authors.forename: Brian
authors.initials: B
authors.lastname: Shaw
mesh_terms: D001233:Aspergillus nidulans / Q000235:genetics*; D002453:Cell Cycle / Q000235:genetics; D004705:Endocytosis / Q000235:genetics; D005656:Fungal Proteins / Q000096:biosynthesis / Q000235:genetics*; D015966:Gene Expression Regulation, Fungal; D025301:Hyphae / Q000201:enzymology / Q000235:genetics*; D049294:Phospholipid Transfer Proteins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D005656:Fungal Proteins; D049294:Phospholipid Transfer Proteins
keywords: Endocytosis; Hypha; Microscopy; Polarization; Secretion