title: Investigation of Osteoinductive Effects of Different Compositions of Bioactive Glass Nanoparticles for Bone Tissue Engineering.
abstract: Bioactive glasses (BG) is one of the well-known materials that used as dental and bone implants, for this reason it is always interesting for researchers has been to increase BG efficiency in the bone tissue engineering. The aim of this study was to evaluate the osteoinductivity of BG different composition nanoparticles with SiO2-CaO-P2O5. The 45S, 58S, and 63S compositions were prepared via the sol-gel technique. Characterization techniques such as x-ray diffraction, field emission scanning electron microscopy (FE-SEM), and laser Doppler electrophoresis (LDE) were used. The osteoinductive capacity of prepared nanoparticles was investigated using unrestricted somatic stem cells (USSC). The particle size of the samples with an amorphous structure mainly ranged less than 40 nm. The zeta potential was negative for all compositions in distilled water at pH 7.4. Bioactive glass nanoparticles were shown to support proliferation of USSC, as shown by microculture tetrazolium (MTT) assay. During osteogenic differentiation, significantly highest values of alkaline phosphatase (ALP) activity and biomineralization were observed on 45S BG. Subsequently, these markers were measured in higher amounts in USSC on 58S and 63S BG compared with tissue culture polystyrene. The nanometric particle size, osteoinductivity, and negative zeta potential make this material a possible candidate for bone tissue engineering applications.
authors.affiliation: From the *Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan, Iran; †Materials Department, Engineering Faculty, Shahrekord University, Shahrekord, Iran; ‡Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran; and §Department of Tissue engineering and Regenerative Medicine, School of Advanced Technologies in Medicine, ShahidBeheshti University of Medical Sciences, Tehran, Iran.
authors.forename: AmirHossein
authors.initials: A
authors.lastname: Tavakolizadeh
authors.forename: Mehdi
authors.initials: M
authors.lastname: Ahmadian
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Fathi
authors.forename: Ali
authors.initials: A
authors.lastname: Doostmohammadi
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
mesh_terms: D001672:Biocompatible Materials / Q000032:analysis* / Q000737:chemistry; D001842:Bone and Bones / Q000502:physiology*; D017610:Calcium Compounds / Q000032:analysis; D005898:Glass / Q000032:analysis* / Q000737:chemistry; D006801:Humans; D053758:Nanoparticles / Q000032:analysis* / Q000737:chemistry; D010012:Osteogenesis; D010087:Oxides / Q000032:analysis; D010316:Particle Size; D012822:Silicon Dioxide / Q000032:analysis; D023822:Tissue Engineering / Q000379:methods*
chemical_list: D001672:Biocompatible Materials; D017610:Calcium Compounds; D010087:Oxides; C570103:bioactive glass 63S; D012822:Silicon Dioxide; C016538:lime