title: Cubosomes for in vivo fluorescence lifetime imaging.
abstract: Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.
authors.affiliation: Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', Trieste, Italy.
authors.forename: Stefania
authors.initials: S
authors.lastname: Biffi
authors.forename: Laura
authors.initials: L
authors.lastname: Andolfi
authors.forename: Claudia
authors.initials: C
authors.lastname: Caltagirone
authors.forename: Chiara
authors.initials: C
authors.lastname: Garrovo
authors.forename: Angela M
authors.initials: AM
authors.lastname: Falchi
authors.forename: Vito
authors.initials: V
authors.lastname: Lippolis
authors.forename: Andrea
authors.initials: A
authors.lastname: Lorenzon
authors.forename: Paolo
authors.initials: P
authors.lastname: Macor
authors.forename: Valeria
authors.initials: V
authors.lastname: Meli
authors.forename: Maura
authors.initials: M
authors.lastname: Monduzzi
authors.forename: Marc
authors.initials: M
authors.lastname: Obiols-Rabasa
authors.forename: Luca
authors.initials: L
authors.lastname: Petrizza
authors.forename: Luca
authors.initials: L
authors.lastname: Prodi
authors.forename: Antonella
authors.initials: A
authors.lastname: Rosa
authors.forename: Judith
authors.initials: J
authors.lastname: Schmidt
authors.forename: Yeshayahu
authors.initials: Y
authors.lastname: Talmon
authors.forename: Sergio
authors.initials: S
authors.lastname: Murgia
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D002470:Cell Survival / Q000187:drug effects; D004339:Drug Compounding / Q000379:methods; D004912:Erythrocytes / Q000187:drug effects; D005260:Female; D005456:Fluorescent Dyes / Q000737:chemistry* / Q000493:pharmacokinetics / Q000494:pharmacology; D005989:Glycerides / Q000737:chemistry; D006801:Humans; D007275:Injections, Intravenous; D008081:Liposomes / Q000138:chemical synthesis / Q000493:pharmacokinetics*; D008099:Liver / Q000187:drug effects / Q000378:metabolism / Q000648:ultrastructure; D051379:Mice; D008807:Mice, Inbred BALB C; D041681:NIH 3T3 Cells; D053758:Nanoparticles / Q000008:administration & dosage / Q000737:chemistry*; D061848:Optical Imaging / Q000379:methods*; D010316:Particle Size; D019265:Spectroscopy, Near-Infrared / Q000379:methods*; D059008:Time-Lapse Imaging
chemical_list: C098793:CY5.5 cyanine dye; D002232:Carbocyanines; D005456:Fluorescent Dyes; D005989:Glycerides; D008081:Liposomes; C005953:monoolein