title: [Role of Non-classical NF-κB Signaling Pathway in B Acute Lymphoblastic Leukemia].
abstract: OBJECTIVE
  To study the role of non-classical NF-κB signaling pathway in B acute lymphoblastic leukemia (B-ALL).
  
  
  METHODS
  The bone marrow samples from 48 patients with B-ALL were collected from March 2015 to March 2016. The real-time quantitative RT-PCR was used for determing mRNA expression levels of NF-κB family members; the NF-κB DNA binding activity in B-ALL cell nucleus was analyzed by ELISA; the apoptosis rate of B-ALL cells alone or co-cultured with bone marrow stromal cells (hBMSC) was determined by flow cytometry.
  
  
  RESULTS
  Relative expression level of mRNA for NF-κB family members, including the Rel A, Rel B, P50 and P52 in ALL-B group was statistically significantly higher than that in normal control group (P<0.05). The clinical characteristics of B-ALL patients with different NF-κB activity were not significantly different (P> 0.05); after B-ALL cells cultured alone or co-cultured, the apoptosis rate of Rel A+/ Rel B-group was statistically significantly higher than that in Rel A+/ Rel B+group (P<0.05); the apoptosis rate of B-ALL cells cultured alone or co-cultured with hBMSC was significantly different.
  
  
  CONCLUSIONS
  Non-classical NF-κB signal marked by Rel B can be used as a new target for B-ALL treatment.
authors.affiliation: Department of Hematology, Baoding Municipal First Central Hospital, Baoding 071000, Hebei Province, China. E-mail: zhuwenjuan11111@163.com.
authors.forename: Xiao-Lei
authors.initials: XL
authors.lastname: Liu
authors.affiliation: Department of Hematology, Baoding Municipal First Central Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Hematology, Baoding Municipal First Central Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Bao-Jing
authors.initials: BJ
authors.lastname: Li
authors.affiliation: Department of Hematology, Baoding Municipal First Central Hospital, Baoding 071000, Hebei Province, China.
authors.forename: Wei-Bo
authors.initials: WB
authors.lastname: Huang
mesh_terms: D017209:Apoptosis; D006801:Humans; D016328:NF-kappa B; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma; D015398:Signal Transduction; D051996:Transcription Factor RelA
chemical_list: D016328:NF-kappa B; C498143:RELA protein, human; D051996:Transcription Factor RelA