title: Improvement of desulfurizing activity of haloalkaliphilic Thialkalivibrio versutus SOB306 with the expression of Vitreoscilla hemoglobin gene.
abstract: OBJECTIVE
  To construct efficient transformation and expression system and further improve desulfurizing activity of cells through expression of Vitreoscilla hemoglobin (VHb) in haloalkaliphilic Thialkalivibrio versutus SOB306.
  
  
  RESULTS
  We transferred plasmids pKT230 and pBBR-smrinto T. versutus SOB306 via a conjugation method. We identified four promoters from among several predicted promoters by scoring for streptomycin resistance, and finally selected tac and p3 based on the efficiency of expression of red fluorescent protein (RFP). Expression of RFP when regulated by tac was more than three times that of p3 in SOB306. Further, we expressed VHb under the control of tac promoter in SOB306. Expression of VHb was verified using CO-difference spectra. The results showed that VHb expression can boost sulfur metabolism, as evidenced by an increase of about 11.7 ± 1.8% in the average rate of thiosulfate removal in the presence of VHb.
  
  
  CONCLUSIONS
  A conjugation transfer and an expression system for Thialkalivibrio, has been developed for the first time and used for expression of VHb to improve desulfurizing activity.
authors.affiliation: State Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100190, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Tingzhen
authors.initials: T
authors.lastname: Mu
authors.affiliation: State Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100190, People's Republic of China.
authors.forename: Maohua
authors.initials: M
authors.lastname: Yang
authors.affiliation: University of Science & Technology Beijing, 30 Xueyuan Road, Haidian District, Beijing, 100083, People's Republic of China.
authors.forename: Jixiang
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100190, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Moustafa Mohammed
authors.initials: MM
authors.lastname: Sharshar
authors.affiliation: State Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100190, People's Republic of China.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China.
authors.forename: Jiangnan
authors.initials: J
authors.lastname: Tian
authors.affiliation: State Key Laboratory of Biochemical Engineering and Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100190, People's Republic of China. jmxing@ipe.ac.cn.|University of Chinese Academy of Sciences, Beijing, 100049, People's Republic of China. jmxing@ipe.ac.cn.
authors.forename: Jianmin
authors.initials: J
authors.lastname: Xing
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics* / Q000378:metabolism; D003227:Conjugation, Genetic; D042001:Ectothiorhodospiraceae / Q000235:genetics* / Q000254:growth & development; D004926:Escherichia coli / Q000235:genetics; D005453:Fluorescence; D015870:Gene Expression; D011401:Promoter Regions, Genetic; D013057:Spectrum Analysis; D013455:Sulfur / Q000378:metabolism*; D054793:Truncated Hemoglobins / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D054793:Truncated Hemoglobins; C082613:hemoglobin protein, Vitreoscilla; D013455:Sulfur
keywords: Biodesulfurization; Conjugative transfer; Desulfurization; Gene expression; Hemoglobin; Vitreoscilla hemoglobin