title: CMC stabilized nano silver synthesis, characterization and its antibacterial and synergistic effect with broad spectrum antibiotics.
abstract: In the present study silver nanoparticles were synthesized by reduction of AgNO3using aqueous CMC solution, which acts as both reducing and capping agent. The formation of AgNO3nanoparticles was observed visually by color change and these nanoparticles were characterized through UV-vis spectroscopy, FTIR, XRD, SEM, EDS and AFM. The FTIR peaks observed to be ranging from 3300 to 605cm-1. The AFM image clearly showed the surface morphology of well dispersed nanoparticles. SEM image illustrates the nanoparticles with spherical shape. The crystalline nature of the particles was assured by XRD analysis. The antimicrobial activity of nanoparticles was tested against human bacterial pathogens (Bacillus cereus, Staphylococcus aureus, S. epidermidis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhimurium &Vibrio vulnificus). The bacterial growth was highly inhibited by the nanoparticles. The synergistic effect of nanoparticles in combination with selected broad spectrum antibiotics against the tested bacteria determined strong growth inhibitory activity.
authors.affiliation: Research Department of Zoology, VHNSN College (Autonomous), Virudhunagar, Tamilnadu, 626001, India. Electronic address: prema.drprema@gmail.com.
authors.forename: P
authors.initials: P
authors.lastname: Prema
authors.affiliation: Department of Zoology, PSG College of Arts and Science, Coimbatore- 14, Tamilnadu, 626001, India.
authors.forename: S
authors.initials: S
authors.lastname: Thangapandiyan
authors.affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam, Kanyakumari District, Tamilnadu, 629502, India.
authors.forename: G
authors.initials: G
authors.lastname: Immanuel
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D004357:Drug Synergism; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D012834:Silver
chemical_list: D000900:Anti-Bacterial Agents; D012834:Silver
keywords: Antibacterial activity; CMC; FTIR; SEM; Silver nanoparticles; XRD