title: Gradient and Patterned Protein Films Stabilized via Nanoimprint Lithography for Engineered Interactions with Cells.
abstract: Protein-based biomaterials provide versatile scaffolds for generating functional surfaces for biomedical applications. However, tailoring the functional and biological properties of protein films remains a challenge. Here, we describe a high-throughput method to designing stable, functional biomaterials by combining inkjet deposition of protein inks with a nanoimprint lithography based methodology. The translation of the intrinsically charged proteins into functional materials properties was demonstrated through controlled cellular adhesion. This modular strategy offers a rapid method to produce customizable biomaterials.
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Li-Sheng
authors.initials: LS
authors.lastname: Wang
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Bradley
authors.initials: B
authors.lastname: Duncan
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Rui
authors.identifier: 0000-0002-3161-801X
authors.initials: R
authors.lastname: Tang
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Yi-Wei
authors.initials: YW
authors.lastname: Lee
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Brian
authors.initials: B
authors.lastname: Creran
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Sukru Gokhan
authors.initials: SG
authors.lastname: Elci
authors.affiliation: Department of Mechanical and Industrial Engineering, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Jiaxin
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Gülen
authors.initials: G
authors.lastname: Yesilbag Tonga
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Jesse
authors.initials: J
authors.lastname: Doble
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Matthew
authors.initials: M
authors.lastname: Fessenden
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Mahin
authors.initials: M
authors.lastname: Bayat
authors.affiliation: Department of Mechanical and Industrial Engineering, University of Massachusetts , Amherst, Massachusetts 01003, United States.
authors.forename: Stephen
authors.initials: S
authors.lastname: Nonnenmann
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Richard W
authors.identifier: 0000-0003-4514-0210
authors.initials: RW
authors.lastname: Vachet
authors.affiliation: Department of Chemistry, University of Massachusetts-Amherst , 710 North Pleasant Street, Amherst, Massachusetts 01003, United States.
authors.forename: Vincent M
authors.identifier: 0000-0002-5184-5439
authors.initials: VM
authors.lastname: Rotello
mesh_terms: D001672:Biocompatible Materials; D002448:Cell Adhesion; D006801:Humans; D049329:Nanostructures; D011327:Printing; D011506:Proteins; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D011506:Proteins
keywords: biomaterial; cell patterning; inkjet printing; nanoimprint lithography; thin films