title: Effect of Lactobacillus reuteri on Cell Viability and PGE2Production in Human Gingival Fibroblasts.
abstract: Emerging evidence suggests that probiotic therapy can play a role in the prevention and management of oral inflammatory diseases through immunomodulation and down-regulation of the inflammatory cascade. The aim of this in vitro study was to investigate the viability of human gingival fibroblasts (HGF) and its production of prostaglandin E2(PGE2), when exposed to supernatants of two mixed Lactobacillus reuteri strains (ATCC PTA 5289 and DSM 17938). The experiments were conducted in the presence and absence of the pro-inflammatory cytokine IL-1β. L. reuteri strains were grown and the bacterial supernatant was collected. The cell-free supernatant was diluted to concentrations equivalent to the ones produced by 0.5 to 5.0 × 107CFU/mL bacteria. Cell viability was assessed with the MTT colorimetric assay and the amount of PGE2in the cell culture medium was determined using the monoclonal enzyme immune assay kits. Our findings showed that none of the L. reuteri supernatants were cytotoxic or affected the viability of HGF. The most concentrated bacterial supernatant stimulated the production of PGE2by the gingival cells in a significant way in the presence of IL-1β (p < 0.05), suggesting that bacterial products secreted from L. reuteri might play a role in the resolution of inflammation in HGF. Thus, our findings justify further investigations on the influence of probiotic bacteria on gingival inflammatory reactions.
authors.affiliation: Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Nørre Allé 20, DK-2200, Copenhagen N, Denmark. gcrubio@sund.ku.dk.
authors.forename: Gina
authors.identifier: http://orcid.org/0000-0002-4451-9563
authors.initials: G
authors.lastname: A Castiblanco
authors.affiliation: Division of Periodontology, Department of Dental Medicine, Karolinska Institutet, Alfred Nobels Allé 8, SE-141 52, Huddinge, Sweden.
authors.forename: Tulay
authors.initials: T
authors.lastname: Yucel-Lindberg
authors.affiliation: Department of Microbiology, Uppsala BioCenter, Swedish University of Agricultural Sciences, Almas Allé 8, Box 7025, SE-750 07, Uppsala, Sweden.
authors.forename: Stefan
authors.initials: S
authors.lastname: Roos
authors.affiliation: Department of Odontology, Faculty of Health and Medical Sciences, University of Copenhagen, Nørre Allé 20, DK-2200, Copenhagen N, Denmark.
authors.forename: Svante
authors.initials: S
authors.lastname: Twetman
mesh_terms: D002470:Cell Survival; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D015232:Dinoprostone / Q000378:metabolism*; D005347:Fibroblasts / Q000166:cytology / Q000382:microbiology*; D005881:Gingiva / Q000166:cytology*; D006801:Humans; D007249:Inflammation / Q000628:therapy; D052200:Limosilactobacillus reuteri / Q000502:physiology*; D019936:Probiotics
chemical_list: D016207:Cytokines; D015232:Dinoprostone
keywords: Fibroblasts; Lactobacillus reuteri; Wound healing