title: A disposable amperometric dual-sensor for the detection of hemoglobin and glycated hemoglobin in a finger prick blood sample.
abstract: A disposable microfluidic amperometric dual-sensor was developed for the detection of glycated hemoglobin (HbA1C) and total hemoglobin (Hb), separately, in a finger prick blood sample. The accurate level of total Hb was determined through the measurements of the cathodic currents of total Hb catalyzed by a toluidine blue O (TBO)-modified working electrode. Subsequently, after washing unbound Hb in the fluidic channel of dual sensor with PBS, the cathodic current by only HbA1Ccaptured on aptamer was monitored using another aptamer/TBO-modified working electrode in the channel. To modify the sensor probe, poly(2,2´:5´,5″-terthiophene-3´-p-benzoic acid) and a multi-wall carbon nanotube (MWCNT) composite layer (pTBA@MWCNT) was electropolymerized on a screen printed carbon electrode (SPCE), followed by immobilization of TBO for the total Hb probe and aptamer/TBO for the HbA1Cprobe, respectively. The characterization of each sensor surface was performed using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), X-ray photoelectron spectroscopy (XPS), quartz crystal microbalance (QCM), field-emission scanning electron microscopy (FE-SEM), and transmission electron microscopy (TEM). The experimental conditions affecting the analytical signal were optimized in terms of the amount of TBO, pH, temperature, binding time, applied potential, and the content ratio of monomer and MWCNT. The dynamic ranges of Hb and HbA1Cwere from 0.1 to 10µM and from 0.006 to 0.74µM, with detection limits of 82(±4.2)nM and 3.7(±0.8)nM, respectively. The reliability of the proposed microfluidic dual-sensor for a finger prick blood sample (1µL) was evaluated in parallel with a conventional method (HPLC) for point-of-care analysis.
authors.affiliation: Department of Chemistry, Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 46241, South Korea.
authors.forename: Jong-Min
authors.initials: JM
authors.lastname: Moon
authors.affiliation: Department of Chemistry, Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 46241, South Korea.
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Dong-A University, College of Medicine, Busan 602-714, South Korea.
authors.forename: Moo Hyun
authors.initials: MH
authors.lastname: Kim
authors.affiliation: Department of Biomedical Engineering, Dong-A University, College of Medicine, Busan 602-714, South Korea.
authors.forename: Jin-Yeong
authors.initials: JY
authors.lastname: Han
authors.affiliation: Department of Biomedical Engineering, Dong-A University, College of Medicine, Busan 602-714, South Korea.
authors.forename: Dong-Keun
authors.initials: DK
authors.lastname: Jung
authors.affiliation: Department of Chemistry, Institute of BioPhysio Sensor Technology (IBST), Pusan National University, Busan 46241, South Korea. Electronic address: ybshim@pusan.ac.kr.
authors.forename: Yoon-Bo
authors.initials: YB
authors.lastname: Shim
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation*; D004396:Coloring Agents / Q000737:chemistry; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation*; D004566:Electrodes; D006442:Glycated Hemoglobin / Q000032:analysis*; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000191:economics / Q000295:instrumentation*; D008958:Models, Molecular; D019095:Point-of-Care Systems / Q000191:economics; D015203:Reproducibility of Results; D014048:Tolonium Chloride / Q000737:chemistry
chemical_list: D052157:Aptamers, Nucleotide; D004396:Coloring Agents; D006442:Glycated Hemoglobin; D006454:Hemoglobins; D014048:Tolonium Chloride
keywords: Aptamer; Glycated hemoglobin; Hemoglobin; Microfluidic dual-sensor; Toluidine blue O