title: Genetic characterization of a second novel picornavirus from an amphibian host, smooth newt (Lissotriton vulgaris).
abstract: In this study, a novel picornavirus was identified in faecal samples from smooth newts (Lissotriton vulgaris). The complete genome of picornavirus strain newt/II-5-Pilis/2014/HUN (KX463670) is 7755 nt long with type-IV IRES and has 39.6% aa sequence identity in the protein P1 to the corresponding protein of bat picornavirus (KJ641686, unassigned) and 42.7% and 53.5% aa sequence identity in the 2C and 3CD protein, respectively, to oscivirus (GU182410, genus Oscivirus). Interestingly, the L-protein of newt/II-5-Pilis/2014/HUN has conserved aa motifs that are similar to those found in phosphatase-1 catalytic (PP1C) subunit binding region (pfam10488) proteins. This second amphibian-origin picornavirus could represent a novel species and could be a founding member of a potential novel picornavirus genus.
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pecs, Hungary.|Department of Medical Microbiology and Immunology, University of Pécs, Szigeti út 12., Pecs, 7624, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Pankovics
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pecs, Hungary.|Department of Medical Microbiology and Immunology, University of Pécs, Szigeti út 12., Pecs, 7624, Hungary.
authors.forename: Ákos
authors.initials: Á
authors.lastname: Boros
authors.affiliation: Lendület Evolutionary Ecology Research Group, Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Tóth
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA.
authors.forename: Tung Gia
authors.initials: TG
authors.lastname: Phan
authors.affiliation: Blood Systems Research Institute, San Francisco, CA, USA.|University of California, San Francisco, CA, USA.
authors.forename: Eric
authors.initials: E
authors.lastname: Delwart
authors.affiliation: Regional Laboratory of Virology, National Reference Laboratory of Gastroenteric Viruses, ÁNTSZ Regional Institute of State Public Health Service, Pecs, Hungary. reuter.gabor@gmail.com.|Department of Medical Microbiology and Immunology, University of Pécs, Szigeti út 12., Pecs, 7624, Hungary. reuter.gabor@gmail.com.
authors.forename: Gábor
authors.initials: G
authors.lastname: Reuter
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D016679:Genome, Viral; D008969:Molecular Sequence Data; D010802:Phylogeny; D010849:Picornaviridae / Q000737:chemistry / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D010850:Picornaviridae Infections / Q000662:veterinary* / Q000821:virology; D012367:RNA, Viral / Q000235:genetics; D012455:Salamandridae / Q000821:virology*; D016415:Sequence Alignment; D014764:Viral Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D012367:RNA, Viral; D014764:Viral Proteins