title: Rat astrocytes are more supportive for mouse OPC self-renewal than mouse astrocytes in culture.
abstract: Mouse primary oligodendrocyte precursor cells (OPCs) are increasingly used to study the molecular mechanisms underlying the phenotype changes in oligodendrocyte differentiation and axonal myelination observed in transgenic or mutant mouse models. However, mouse OPCs are much more difficult to be isolated by the simple dissociation culture of brain tissues than their rat counterparts. To date, the mechanisms underlying the species difference in OPC preparation remain obscure. In this study, we showed that astrocytes from rats have a stronger effect than those from mouse in promoting OPC proliferation and survival in vitro. Mouse astrocytes displayed significantly weaker viability in culture and reduced potential in maintaining OPC self-renewal, as confirmed by culturing OPCs with conditioned media from rat or mouse astrocytes. These results explained the reason for why stratified cultures of OPCs and astrocytes are difficult to be achieved in mouse CNS tissues. Based on these findings, we adopted inactivated rat astrocytes as feeder cells to support the self-renewal of mouse cortical OPCs and preparation of high-purity mouse OPCs. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 907-916, 2017.
authors.affiliation: Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Cheng
authors.affiliation: Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Xie
authors.affiliation: Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.
authors.forename: Jiajun
authors.initials: J
authors.lastname: Qi
authors.affiliation: Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.
authors.forename: Xiaofeng
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.
authors.forename: Zunyi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.|Department of Anatomical Sciences and Neurobiology, University of Louisville, Louisville, Kentucky, 40292.
authors.forename: Mengsheng
authors.initials: M
authors.lastname: Qiu
authors.affiliation: Zhejiang Key Laboratory of Organ Development and Regeneration, College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, 310036, China.
authors.forename: Junlin
authors.initials: J
authors.lastname: Yang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology; D001253:Astrocytes / Q000166:cytology / Q000502:physiology*; D049109:Cell Proliferation / Q000502:physiology; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D002540:Cerebral Cortex / Q000502:physiology; D018920:Coculture Techniques; D017077:Culture Media, Conditioned; D051379:Mice / Q000502:physiology*; D058953:Neural Stem Cells / Q000166:cytology / Q000502:physiology*; D009836:Oligodendroglia / Q000166:cytology / Q000502:physiology*; D051381:Rats / Q000502:physiology*; D013045:Species Specificity
chemical_list: D017077:Culture Media, Conditioned
keywords: conditioned medium; differentiation; feeder cells; stratified culture; survival