title: Glycosyl flavonoid profile, in vivo antidiabetic and in vitro antioxidant properties of Linaria reflexa Desf.
abstract: Aerial parts of Linaria reflexa, used in North African folk medicine for treating certain skin diseases, were investigated by HPLC-DAD-ESI/MS technique able to identify the glycosyl flavonoids pectolinarin (1), isolinariin A (2), isolinariin B (3), linariin (4), isolinariin D (5) and isolinariin E (6) as the most abundant components in both hydroalcoholic (HAE) and ultrasound-assisted (UAE) extracts profiles. Metabolite 5, isolated and fully characterised by extensive nuclear magnetic resonance (NMR) analysis, has been very recently reported from L. japonica together with the compound 6. Good antioxidant activities (DPPH radical scavenging, β-carotene bleaching and reducing power assays) were observed for the extracts. The remarkable antidiabetic activity displayed by UAE (300 mg/kg) has yielded the most marked decrease in blood glucose levels of the alloxan diabetic rats (-72.09%), greater than the effects by the drug glybenclamide (-63.29%). This study reports the first correlation of antidiabetic activity of Linaria sp. extracts with their chemical composition.
authors.affiliation: a Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine BP , Constantine , Algeria.|b Faculté des Sciences, Département de Chimie , Université de M'Sila , M'Sila , Algeria.
authors.forename: Thamere
authors.identifier: http://orcid.org/0000-0001-5305-1360
authors.initials: T
authors.lastname: Cheriet
authors.affiliation: c Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie , Université des Frères Mentouri Constantine BP , Constantine , Algeria.
authors.forename: Mourad
authors.initials: M
authors.lastname: Hanfer
authors.affiliation: d Faculté des Sciences, Département de Microbiologie et Biochimie , Université de M'Sila , M'Sila , Algeria.
authors.forename: Amel
authors.initials: A
authors.lastname: Boudjelal
authors.affiliation: d Faculté des Sciences, Département de Microbiologie et Biochimie , Université de M'Sila , M'Sila , Algeria.
authors.forename: Nadir
authors.initials: N
authors.lastname: Baali
authors.affiliation: e Laboratorio di ChimicaBioorganica , Università di Trento , Povo-Trento , Italy.
authors.forename: Ines
authors.initials: I
authors.lastname: Mancini
authors.affiliation: a Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine BP , Constantine , Algeria.
authors.forename: Ramdane
authors.initials: R
authors.lastname: Seghiri
authors.affiliation: c Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie , Université des Frères Mentouri Constantine BP , Constantine , Algeria.
authors.forename: Souad
authors.initials: S
authors.lastname: Ameddah
authors.affiliation: c Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie , Université des Frères Mentouri Constantine BP , Constantine , Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Menad
authors.affiliation: a Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine BP , Constantine , Algeria.
authors.forename: Fadila
authors.initials: F
authors.lastname: Benayache
authors.affiliation: a Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine BP , Constantine , Algeria.
authors.forename: Samir
authors.initials: S
authors.lastname: Benayache
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000494:pharmacology*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D002867:Chromones / Q000737:chemistry / Q000494:pharmacology; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D004353:Drug Evaluation, Preclinical / Q000379:methods; D005419:Flavonoids / Q000737:chemistry* / Q000494:pharmacology; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D032221:Linaria / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D016488:Medicine, African Traditional; D010936:Plant Extracts / Q000737:chemistry; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D019207:beta Carotene / Q000378:metabolism
chemical_list: D000975:Antioxidants; D002867:Chromones; D005419:Flavonoids; D007004:Hypoglycemic Agents; D010936:Plant Extracts; D019207:beta Carotene; C052786:pectolinarin
keywords: HPLC–DAD–ESI/MS; Linaria reflexa; antidiabetic; antioxidant; isolinariin D; isolinariin E