title: Rapid determination of alkaloids in Macleaya cordata using ionic liquid extraction followed by multiple reaction monitoring UPLC-MS/MS analysis.
abstract: The ultrasonic-assisted extraction (UAE) and ionic liquid based dispersive liquid-liquid microextraction (IL-DLLME) have been successfully applied in extracting of six alkaloids from M. cordata. 1-hexyl-3-methylimidazolium tetrafluoroborate ([C6MIM][BF4]) aqueous solution was used as extraction solvent. The target analytes in raw material were deposited into a single drop of 1-hexyl-3-methylimidazolium hexafluorophosphate ([C6MIM][PF6]), which was in situ formed by mixing [C6MIM][BF4] and potassium hexafluorophosphate ([K][PF6]. Afterwards, the extract was analyzed by ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) in multiple-reaction monitoring (MRM) mode. The proposed method was fully validated in terms of linearity (0.9983-0.9992), LOD (0.080ngmL-1), LOQ (0.25ngmL-1), intra-day precision (<5.46%), inter-day precision (<6.36%), and recovery (86.42-112.48%). The results indicate that the approach of combining IL-DLLME with UPLC-MS/MS is powerful and practical for analyzing alkaloids in M. cordata., and it also has great potential for comprehensive quality control of other herbal medicines.
authors.affiliation: School of Public Health, Guangdong Medical University, Dongguan, China.
authors.forename: Linqiu
authors.initials: L
authors.lastname: Li
authors.affiliation: School of Public Health, Guangdong Medical University, Dongguan, China.
authors.forename: Mingyuan
authors.initials: M
authors.lastname: Huang
authors.affiliation: School of Public Health, Guangdong Medical University, Dongguan, China.
authors.forename: Junli
authors.initials: J
authors.lastname: Shao
authors.affiliation: School of Public Health, Guangdong Medical University, Dongguan, China.
authors.forename: Bokun
authors.initials: B
authors.lastname: Lin
authors.affiliation: Institute of Seafood, Zhejiang Gongshang University, Hangzhou, China. Electronic address: leonqshen@163.com.
authors.forename: Qing
authors.initials: Q
authors.lastname: Shen
mesh_terms: D000470:Alkaloids / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D052578:Ionic Liquids / Q000737:chemistry*; D059625:Liquid-Liquid Extraction / Q000379:methods*; D028101:Papaveraceae; D035261:Plant Components, Aerial / Q000737:chemistry; D010936:Plant Extracts / Q000032:analysis*; D018517:Plant Roots / Q000737:chemistry; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013997:Time Factors
chemical_list: D000470:Alkaloids; D052578:Ionic Liquids; D010936:Plant Extracts
keywords: Alkaloids; Liquid based dispersive liquid–liquid microextraction; Macleaya cordata; Multiple-reaction monitoring; UPLC–MS/MS