title: Hydronephrosis with ureteritis developed in C57BL/6N mice carrying the congenic region derived from MRL/MpJ-type chromosome 11.
abstract: Inbred MRL/MpJ mice show several unique phenotypes in tissue regeneration processes and the urogenital and immune systems. Clarifying the genetic and molecular bases of these phenotypes requires the analysis of their genetic susceptibility locus. Herein, hydronephrosis development was incidentally observed in MRL/MpJ-derived chromosome 11 (D11Mit21-212)-carrying C57BL/6N-based congenic mice, which developed bilateral or unilateral hydronephrosis in both males and females with 23.5% and 12.5% prevalence, respectively. Histopathologically, papillary malformations of the transitional epithelium in the pelvic-ureteric junction seemed to constrict the ureter luminal entrance. Characteristically, eosinophilic crystals were observed in the lumen of diseased ureters. These ureters were surrounded by infiltrating cells mainly composed of numerous CD3+T-cells and B220+B-cells. Furthermore, several Iba-1+macrophages, Gr-1+granulocytes, mast cells and chitinase 3-like 3/Ym1 (an important inflammatory lectin)-positive cells were detected. Eosinophils also accumulated to these lesions in diseased ureters. Some B6.MRL-(D11Mit21-D11Mit212) mice had duplicated ureters. We determined >100 single nucleotide variants between C57BL/6N- and MRL/MpJ-type chromosome 11 congenic regions, which were associated with nonsynonymous substitution, frameshift or stopgain of coding proteins. In conclusion, B6.MRL-(D11Mit21-D11Mit212) mice spontaneously developed hydronephrosis due to obstructive uropathy with inflammation. Thus, this mouse line would be useful for molecular pathological analysis of obstructive uropathy in experimental medicine.
authors.affiliation: a Laboratory of Anatomy , Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo , Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Ichii
authors.affiliation: b R&D Department, Daiichi Sankyo Healthcare Co., Ltd. , Tokyo , Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Chihara
authors.affiliation: c Department of Anatomy , Research Institute of Medical Science, Konkuk University School of Medicine , Seoul , Republic of Korea.
authors.forename: Shin-Hyo
authors.initials: SH
authors.lastname: Lee
authors.affiliation: a Laboratory of Anatomy , Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo , Japan.|d Section of Biological Safety Research, Chitose Laboratory, Japan Food Research Laboratories , Chitose , Japan.
authors.forename: Teppei
authors.initials: T
authors.lastname: Nakamura
authors.affiliation: a Laboratory of Anatomy , Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo , Japan.
authors.forename: Saori
authors.initials: S
authors.lastname: Otsuka-Kanazawa
authors.affiliation: e Department of Endocrinology , Metabolism and Nephrology, Kochi Medical School, Kochi University , Nankoku , Japan , and.
authors.forename: Taro
authors.initials: T
authors.lastname: Horino
authors.affiliation: a Laboratory of Anatomy , Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo , Japan.|f Department of Histology and Cytology , Faculty of Veterinary Medicine, Zagazig University , Zagazig , Egypt.
authors.forename: Yaser Hosny Ali
authors.initials: YH
authors.lastname: Elewa
authors.affiliation: a Laboratory of Anatomy , Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University , Sapporo , Japan.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Kon
mesh_terms: D000818:Animals; D001706:Biopsy; D033481:Chromosomes, Mammalian; D004195:Disease Models, Animal; D005260:Female; D020022:Genetic Predisposition to Disease; D016678:Genome; D006869:Hydronephrosis / Q000209:etiology* / Q000473:pathology*; D008297:Male; D051379:Mice; D020297:Mice, Congenic; D008810:Mice, Inbred C57BL; D019463:Mice, Inbred MRL lpr; D020641:Polymorphism, Single Nucleotide; D014513:Ureter / Q000473:pathology* / Q000648:ultrastructure; D000073359:Exome Sequencing
keywords: MRL/MpJ; Ureteritis; congenic mice; hydronephrosis; inflammation