title: Effects of MgSO4and magnesium transporters on 6-hydroxydopamine-induced SH-SY5Y cells.
abstract: OBJECTIVE
  The magnesium ion (Mg2+) fulfils several important functions for living organisms. We investigated whether there is a protective effect of MgSO4on 6-OHDA-induced neurotoxicity in SH-SY5Y cells, and gained insight into the effects of cellular mRNA and protein expression of the magnesium transporters SLC41A1, NIPA1, MagT1 and CNNM2 on 6-OHDA-induced neurotoxicity.
  
  
  METHODS
  The effect of MgSO4on cell viability in 6-OHDA-treated SH-SY5Y cells was measured using a CCK-8 kit. The mRNA and protein expression of SLC41A1, NIPA1, MagT1, and CNNM2 were detected using reverse transcription-qPCR and Western blot.
  
  
  RESULTS
  The results showed that SH-SY5Y cells treated with 25-50μM 6-OHDA for 24h significantly decreased cell viability, while if pre-incubated with 0.125-1mM MgSO4for 1h before adding 6-OHDA it partially prevented the cell damage. There was a significant decrease in cellular mRNA and protein expression of SLC41A1, NIPA1, MagT1 and CNNM2 in 6-OHDA treated SH-SY5Y cells, and MgSO4can reverse its decline.
  
  
  CONCLUSIONS
  Our results suggest that MgSO4may protect SH-SY5Y cells against 6-OHDA-induced cell injury and that gene expression of SLC41A1, NIPA1, MagT1, and CNNM2 might be involved in dopaminergic neurons.
authors.affiliation: Key Laboratory of Brain Aging and Neurodegenerative Diseases, Fujian Medical University, Fuzhou 350122, China; Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350122, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Lin
authors.affiliation: Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350122, China.
authors.forename: Zili
authors.initials: Z
authors.lastname: Ke
authors.affiliation: Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350122, China.
authors.forename: Meiqi
authors.initials: M
authors.lastname: Lv
authors.affiliation: Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350122, China.
authors.forename: Renxi
authors.initials: R
authors.lastname: Lin
authors.affiliation: Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350122, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
authors.affiliation: Key Laboratory of Brain Aging and Neurodegenerative Diseases, Fujian Medical University, Fuzhou 350122, China; Department of Biochemistry and Molecular Biology, Fujian Medical University, Fuzhou 350122, China. Electronic address: zhzheng@mail.fjmu.edu.cn.
authors.forename: Zhihong
authors.initials: Z
authors.lastname: Zheng
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D017136:Ion Transport; D008278:Magnesium Sulfate / Q000378:metabolism / Q000494:pharmacology*; D016627:Oxidopamine / Q000009:adverse effects*
chemical_list: D008278:Magnesium Sulfate; D016627:Oxidopamine
keywords: 6-hydroxydopamine; Magnesium transporters; MgSO(4); Parkinson's disease; SH-SY5Y cells