title: Quantification of the IgG2/4 kappa Monoclonal Therapeutic Eculizumab from Serum Using Isotype Specific Affinity Purification and Microflow LC-ESI-Q-TOF Mass Spectrometry.
abstract: As therapeutic monoclonal antibodies (mAbs) become more humanized, traditional tryptic peptide approaches used to measure biologics in serum become more challenging since unique clonotypic peptides used for quantifying the mAb may also be found in the normal serum polyclonal background. An alternative approach is to monitor the unique molecular mass of the intact light chain portion of the mAbs using liquid chromatography-mass spectrometry (LC-MS). Distinguishing a therapeutic mAb from a patient's normal polyclonal immunoglobulin (Ig) repertoire is the primary limiting factor when determining the limit of quantitation (LOQ) in serum. The ability to selectively extract subclass specific Igs from serum reduces the polyclonal background in a sample. We present here the development of an LC-MS method to quantify eculizumab in serum. Eculizumab is a complement component 5 (C5) binding mAb that is fully humanized and contains portions of both IgG2 and IgG4 subclasses. Our group developed a method that uses Life Technologies CaptureSelect IgG4 (Hu) affinity matrix. We show here the ability to quantitate eculizumab with a LOQ of 5 mcg/mL by removing the higher abundance IgG1, IgG2, and IgG3 from the polyclonal background, making this approach a simple and efficient procedure. Graphical Abstract ᅟ.
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA.
authors.forename: Paula M
authors.initials: PM
authors.lastname: Ladwig
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA.
authors.forename: David R
authors.initials: DR
authors.lastname: Barnidge
authors.affiliation: Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN, 55905, USA. willrich.mariaalice@mayo.edu.
authors.forename: Maria A V
authors.initials: MA
authors.lastname: Willrich
mesh_terms: D061067:Antibodies, Monoclonal, Humanized / Q000032:analysis / Q000097:blood* / Q000302:isolation & purification; D002846:Chromatography, Affinity / Q000379:methods*; D006801:Humans; D007074:Immunoglobulin G / Q000302:isolation & purification; D057230:Limit of Detection; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*
chemical_list: D061067:Antibodies, Monoclonal, Humanized; D007074:Immunoglobulin G; C481642:eculizumab
keywords: Eculizumab; Intact light chain; Mass spectrometry; Therapeutic monoclonal antibody