title: A proteomics assay to detect eight CBRN-relevant toxins in food.
abstract: A proteomics assay was set up to analyze food substrates for eight toxins of the CBRN (chemical, biological, radiological and nuclear) threat, namely ricin, Clostridium perfringens epsilon toxin (ETX), Staphylococcus aureus enterotoxins (SEA, SEB and SED), shigatoxins from Shigella dysenteriae and entero-hemorragic Escherichia coli strains (STX1 and STX2) and Campylobacter jejuni cytolethal distending toxin (CDT). The assay developed was based on an antibody-free sample preparation followed by bottom-up LC-MS/MS analysis operated in targeted mode. Highly specific detection and absolute quantification were obtained using isotopically labeled proteins (PSAQ standards) spiked into the food matrix. The sensitivity of the assay for the eight toxins was lower than the oral LD50 which would likely be used in a criminal contamination of food supply. This assay should be useful in monitoring biological threats. In the public-health domain, it opens the way for multiplex investigation of food-borne toxins using targeted LC-MS/MS.
authors.affiliation: Université Grenoble-Alpes, Grenoble, France.|CEA, BIG, Biologie à Grande Echelle, Grenoble, France.|INSERM, U1038, Grenoble, France.|CEA, LETI, Clinatec, Grenoble, France.
authors.forename: Benoit
authors.initials: B
authors.lastname: Gilquin
authors.affiliation: Université Grenoble-Alpes, Grenoble, France.|CEA, BIG, Biologie à Grande Echelle, Grenoble, France.|INSERM, U1038, Grenoble, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Jaquinod
authors.affiliation: Université Grenoble-Alpes, Grenoble, France.|CEA, BIG, Biologie à Grande Echelle, Grenoble, France.|INSERM, U1038, Grenoble, France.
authors.forename: Mathilde
authors.initials: M
authors.lastname: Louwagie
authors.affiliation: Université Grenoble-Alpes, Grenoble, France.|CEA, BIG, Biologie à Grande Echelle, Grenoble, France.|INSERM, U1038, Grenoble, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Kieffer-Jaquinod
authors.affiliation: Université Grenoble-Alpes, Grenoble, France.|CEA, BIG, Biologie à Grande Echelle, Grenoble, France.|INSERM, U1038, Grenoble, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Kraut
authors.affiliation: Université Grenoble-Alpes, Grenoble, France.|CEA, BIG, Biologie à Grande Echelle, Grenoble, France.|INSERM, U1038, Grenoble, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Ferro
authors.affiliation: CEA, iBiTec-S, Laboratoire d'Etude du Métabolisme des Médicaments, Gif-sur-Yvette, France.
authors.forename: François
authors.initials: F
authors.lastname: Becher
authors.affiliation: Université Grenoble-Alpes, Grenoble, France.|CEA, BIG, Biologie à Grande Echelle, Grenoble, France.|INSERM, U1038, Grenoble, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Brun
mesh_terms: D001427:Bacterial Toxins / Q000032:analysis; D002853:Chromatography, Liquid; D004768:Enterotoxins / Q000032:analysis; D040901:Proteomics / Q000379:methods*; D022621:Shiga Toxin / Q000032:analysis; D053719:Tandem Mass Spectrometry
chemical_list: D001427:Bacterial Toxins; C039811:Clostridium perfringens epsilon-toxin; D004768:Enterotoxins; C067044:cytolethal distending toxin; D022621:Shiga Toxin
keywords: Bioterrorism; Food; Mass spectrometry; Quantification; Technology; Toxin