title: Ratiometric Fluorescent Silicon Quantum Dots-Ce6 Complex Probe for the Live Cell Imaging of Highly Reactive Oxygen Species.
abstract: The monitoring of reactive oxygen species (ROS) in living cells remains challenging because of the complexity, short half-life, and autofluorescence of biological samples. In this work, we designed a ratiometric fluorescent probe for the detection and imaging of ROS, which was constructed from silicon quantum dots (Si QDs) with chlorin e6 (Ce6) through electrostatic attraction and showed well-resolved dual fluorescence emission signals (490 and 660 nm). Sensitive and selective biosensing of hydroxyl radical (•OH) was demonstrated on the basis of fluorescence quenching of the Si QDs and Ce6 as an internal reference to avoid environmental interference, with a detection limit of ∼0.97 μM. The endogenous release of•OH was also monitored and imaged in living cells.
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Ren
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Daixin
authors.initials: D
authors.lastname: Ye
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Hui
authors.identifier: http://orcid.org/0000-0002-2281-5935
authors.initials: H
authors.lastname: Chen
authors.affiliation: Department of Chemistry, Fudan University , Shanghai 200433, P. R. China.
authors.forename: Jilie
authors.initials: J
authors.lastname: Kong
mesh_terms: D005456:Fluorescent Dyes; D045663:Quantum Dots; D017382:Reactive Oxygen Species; D012825:Silicon; D013050:Spectrometry, Fluorescence
chemical_list: D005456:Fluorescent Dyes; D017382:Reactive Oxygen Species; D012825:Silicon
keywords: cell imaging; chlorin e6 (Ce6); highly reactive oxygen species; hydroxyl radical (•OH); ratiometric fluorescence probe; silicon quantum dots