title: Immobilization of laccase of Pycnoporus sanguineus CS43.
abstract: Laccase from Pycnoporus sanguineus CS43 was successfully immobilized onto Immobead-150 and Eupergit-C by covalent binding and by entrapment in LentiKats. The highest immobilization was onto Immobead-150 (97.1±1.2%) compared to the other supports, LentiKats (89±1.1%) and Eupergit-C (83.2±1.4%). All three immobilized enzyme systems showed increased thermostability and better mechanical properties than free laccase. Moreover, after 5 cycles of reuse of these systems, 90% of initial laccase activity was retained. Immobead-150 and LentiKats systems exhibited the highest efficiencies in removal of m-cresol under the combined actions of biodegradation and adsorption, while laccase entrapped in LentiKats showed a high ability for degradation of m-cresol within 24h. In addition, the typical Michaelis-Menten enzymatic model effectively described the kinetic profile of m-cresol degradation by the enzyme entrapped in LentiKats. Based on the results obtained in the present study, it can be established that the immobilized biocatalysts developed here possess significant potential for wastewater treatment.
authors.affiliation: Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L., 64849, Mexico.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Gonzalez-Coronel
authors.affiliation: Department of Chemical Engineering, University of Vigo, Campus As Lagoas, Marcosende 36310 Vigo, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Cobas
authors.affiliation: Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L., 64849, Mexico.
authors.forename: Magdalena de J
authors.initials: MJ
authors.lastname: Rostro-Alanis
authors.affiliation: Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L., 64849, Mexico.
authors.forename: Roberto
authors.initials: R
authors.lastname: Parra-Saldívar
authors.affiliation: Laboratorio de Enzimología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Ave. Pedro de Alba S/N Ciudad Universitaria, San Nicolás de los Garza, Nuevo León, C.P. 64450, Mexico.
authors.forename: Carlos
authors.initials: C
authors.lastname: Hernandez-Luna
authors.affiliation: Department of Chemical Engineering, University of Vigo, Campus As Lagoas, Marcosende 36310 Vigo, Spain.
authors.forename: Marta
authors.initials: M
authors.lastname: Pazos
authors.affiliation: Department of Chemical Engineering, University of Vigo, Campus As Lagoas, Marcosende 36310 Vigo, Spain. Electronic address: sanroman@uvigo.es.
authors.forename: M Ángeles
authors.initials: MÁ
authors.lastname: Sanromán
mesh_terms: D000327:Adsorption; D001673:Biodegradation, Environmental; D003408:Cresols; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism* / Q000648:ultrastructure; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D042845:Laccase / Q000378:metabolism* / Q000648:ultrastructure; D055494:Pycnoporus / Q000201:enzymology*; D013696:Temperature
chemical_list: D003408:Cresols; D004800:Enzymes, Immobilized; D042845:Laccase; C042041:3-cresol
keywords: Eupergit-C; Immobead-150; Immobilization; Laccase; LentiKats; Pycnoporus