title: [Effects of Arsenic Trioxide on K562 Cell Proliferation and Its Mechanisms].
abstract: OBJECTIVE
  To investigate the effects of arsenic trioxide (As2O3) on K562 cell proliferation by regulating cell cycle protein D1 and cyclin-dependent kinase inhibitor p27kip1.
  
  
  METHODS
  MTT was used to detect the effect of As2O3on K562 cell proliferation, so as to screen out the appropriate drug concentration. Furthermore, the K562 cell apoptosis was observed by microscopy. The expression of CyclinD1 and p27kip1 in K562 cells treated with As2O3was analyzed by reverse transcription-polymerase chain reaction(RT-PCR), immunohistochemistry and Western blot.
  
  
  RESULTS
  As2O3could inhibit the proliferation of K562 cells in a dose- and time- dependent manner (r= 0.967). And the apoptosis cell number in As2O3group was significantly higher than that in the control group(P<0.05). As2O3could markedly inhibit the expression of CyclinD1 in K562 cells(P<0.05), but the expression of P27kip1 was not significantly changed after As2O3treatment.
  
  
  CONCLUSIONS
  As2O3can induce K562 cell apoptosis and inhibit K562 cell proliferation by regulating the expression of CyclinD1.
authors.affiliation: School of Laboratorial Medicine, Xinxiang Medical University; Collaborative Innovation Center of Molecular Diagnosis and Laboratorial Medicine of Henan Province, Xinxiang 453003, Henan Province, China.
authors.forename: Fan-Ping
authors.initials: FP
authors.lastname: Wang
authors.affiliation: School of Laboratorial Medicine, Xinxiang Medical University,Xinxiang 453003, Henan Province, China.
authors.forename: Jing-Jing
authors.initials: JJ
authors.lastname: Zhang
authors.affiliation: School of Laboratorial Medicine, Xinxiang Medical University,Xinxiang 453003, Henan Province, China.
authors.forename: Li-Min
authors.initials: LM
authors.lastname: Fang
authors.affiliation: School of Laboratorial Medicine, Xinxiang Medical University,Xinxiang 453003, Henan Province, China.
authors.forename: Ya-Li
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: School of Laboratorial Medicine, Xinxiang Medical University,Xinxiang 453003, Henan Province, China.
authors.forename: Lu-Lu
authors.initials: LL
authors.lastname: Chen
authors.affiliation: School of Laboratorial Medicine, Xinxiang Medical University,Xinxiang 453003, Henan Province, China.
authors.forename: Yue-Yuan
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratorial Examination, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
authors.forename: Jun-Peng
authors.initials: JP
authors.lastname: Li
authors.affiliation: School of Laboratorial Medicine, Xinxiang Medical University,Xinxiang 453003, Henan Province, China. E-mail:wmy118@126.com.
authors.forename: Ming-Yong
authors.initials: MY
authors.lastname: Wang
mesh_terms: D000970:Antineoplastic Agents; D017209:Apoptosis; D000077237:Arsenic Trioxide; D001152:Arsenicals; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D006801:Humans; D020014:K562 Cells; D010087:Oxides
chemical_list: D000970:Antineoplastic Agents; D001152:Arsenicals; D010087:Oxides; D000077237:Arsenic Trioxide