title: Comprehensive and Systematic Analysis of the Immunocompatibility of Polyelectrolyte Capsules.
abstract: The immunocompability of polyelectrolyte capsules synthesized by layer-by-layer deposition has been investigated. Capsules of different architecture and composed of either non-degradable or biodegradable polymers, with either positively or negatively charged outer surface, and with micrometer size, have been used, and the capsule uptake by different cell lines has been studied and quantified. Immunocompatibility studies were performed with peripheral blood mononuclear cells (PBMCs). Data demonstrate that incubation with capsules, at concentrations relevant for practical applications, did not result in a reduced viability of cells, as it did not show an increased apoptosis. Presence of capsules also did not result in an increased expression of TNF-α, as detected with antibody staining, as well as at mRNA level. It also did not result in increased expression of IL-6, as detected at mRNA level. These results indicate that the polyelectrolyte capsules used in this study are immunocompatible.
authors.forename: Mikhail V
authors.initials: MV
authors.lastname: Zyuzin
authors.forename: Paula
authors.initials: P
authors.lastname: Díez
authors.affiliation: Laboratory of PrecisonNanoMedicine, Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Department of Materials Science and Engineering, The Iby and Aladar Fleischman Faculty of Engineering, Center for Nanoscience and Nanotechnology, Tel Aviv University , Tel Aviv 6997801, Israel.
authors.forename: Meir
authors.initials: M
authors.lastname: Goldsmith
authors.affiliation: CIC biomaGUNE , Paseo de Miramón 182, 20014 Donostia - San Sebastián, Spain.
authors.forename: Susana
authors.initials: S
authors.lastname: Carregal-Romero
authors.forename: Cristina
authors.initials: C
authors.lastname: Teodosio
authors.forename: Joanna
authors.initials: J
authors.lastname: Rejman
authors.forename: Neus
authors.initials: N
authors.lastname: Feliu
authors.affiliation: Instituto de Ciencia de Materiales de Sevilla, CSIC - Universidad de Sevilla , C. Américo Vespucio 49, E-41092, Seville, Spain.
authors.forename: Alberto
authors.identifier: 0000-0002-0850-6589
authors.initials: A
authors.lastname: Escudero
authors.affiliation: Department of Analytical Chemistry, Nutrition and Food Science, Faculty of Chemistry, University of Salamanca , 37008 Salamanca, Spain.
authors.forename: María Jesús
authors.initials: MJ
authors.lastname: Almendral
authors.forename: Uwe
authors.initials: U
authors.lastname: Linne
authors.affiliation: Laboratory of PrecisonNanoMedicine, Department of Cell Research and Immunology, George S. Wise Faculty of Life Sciences, Department of Materials Science and Engineering, The Iby and Aladar Fleischman Faculty of Engineering, Center for Nanoscience and Nanotechnology, Tel Aviv University , Tel Aviv 6997801, Israel.
authors.forename: Dan
authors.initials: D
authors.lastname: Peer
authors.forename: Manuel
authors.initials: M
authors.lastname: Fuentes
authors.affiliation: CIC biomaGUNE , Paseo de Miramón 182, 20014 Donostia - San Sebastián, Spain.
authors.forename: Wolfgang J
authors.identifier: 0000-0003-1672-6650
authors.initials: WJ
authors.lastname: Parak
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis / Q000187:drug effects; D002214:Capsules; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects*; D002478:Cells, Cultured; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D000071228:Polyelectrolytes / Q000009:adverse effects* / Q000493:pharmacokinetics; D012333:RNA, Messenger / Q000235:genetics; D014409:Tumor Necrosis Factor-alpha / Q000032:analysis / Q000235:genetics / Q000276:immunology
chemical_list: D002214:Capsules; D000071228:Polyelectrolytes; D012333:RNA, Messenger; D014409:Tumor Necrosis Factor-alpha