title: Selenium Administration Alleviates Toxicity of Chromium(VI) in the Chicken Brain.
abstract: Selenium (Se) can play a protective role against heavy metal toxicity. This experiment aims to evaluate the effect of Se supplementation at different doses on the chicken brains. Oxidative stress was induced in the chicken brains by chromium(VI). A total of 105 Hyland brown male chickens were randomly divided into seven groups, including the control group, poisoned group [6%LD50K2Cr2O7body weight (B.W.)], and detoxification groups K2Cr2O7(6%LD50) + Se (0.31, 0.63, 1.25, 2.50, and 5.00 Na2SeO3mg/kg B.W.) orally in water for 42 days. The chickens were detected by the activities of mitochondrial membrane potential, 2'-benzoyloxycinnamaldehyde, superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and Ca2+-ATPase. Cr(VI) administration caused histopathological damage. In addition, changes in oxidative stress indicators were observed in the chicken's brains. Se supplement increased the levels of GSH, mitochondrial membrane potential (MMP), and Ca2+-ATPase and reduced MDA activity in the detoxification groups. However, the high-dose Se supplementation groups of 2.50 and 5.00 mg/kg reduced the activities of GSH, MMP, and Ca2+-ATPase; increased the brain-body ratio; and increased SOD activity. In conclusion, Cr(VI) exposure caused oxidative stress. Se exerted a remission effect on toxic responses in the chicken brains. However, a high Se concentration was synergistic to the toxic effect of Cr(VI).
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors.forename: Pan
authors.initials: P
authors.lastname: Hao
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors.forename: Yiran
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Wan
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China.
authors.forename: Ziqiang
authors.initials: Z
authors.lastname: Cheng
authors.affiliation: College of Veterinary Medicine, Shandong Agricultural University, Tai'an, 271018, People's Republic of China. liuyongxia666@163.com.
authors.forename: Yongxia
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Research Center for Animal Disease Control Engineering Shandong Province, Shandong Agricultural University, Tai'an, 271018, People's Republic of China. Liujz@sdau.edu.cn.
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Liu
mesh_terms: D000818:Animals; D030161:Avian Proteins / Q000378:metabolism; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D002645:Chickens / Q000378:metabolism*; D002857:Chromium / Q000633:toxicity*; D053078:Membrane Potential, Mitochondrial / Q000187:drug effects; D008928:Mitochondria / Q000378:metabolism / Q000473:pathology; D051336:Mitochondrial Membranes / Q000378:metabolism / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects*; D012643:Selenium / Q000494:pharmacology*
chemical_list: D030161:Avian Proteins; D002857:Chromium; C074702:chromium hexavalent ion; D012643:Selenium
keywords: Ca2+-ATPase; Cr(VI); Mitochondrial membrane potential; Oxidative damage; Selenium