title: Identification of Disease-Transmitting Mosquitoes: Development of Species-Specific Probes for DNA Chip Assay Using Mitochondrial COI and ND2 Genes and Ribosomal Internal Transcribed Spacer 2.
abstract: Mosquitoes, which transmit infectious diseases, such as malaria and dengue fever, are harmful to human health. Thus, accurate and rapid identification of vectors is a critical step for the control of mosquito-borne diseases. However, phenotypic variations in adults, lack of recognizable features of the immature, and fragility of mosquitoes make identification difficult. Molecular approaches have been widely applied to identify mosquitoes, yet these methods have been focused only on the identification of a few species. This study used sequences of two mitochondrial genes, COI and ND2, and a ribosomal gene, ITS2, to design species-specific probes. Biochips thus developed were able to provide simultaneous identification of nine important medical and veterinary species, including the immature, from genera of Aedes, Anopheles, Armigeres, and Culex. This chip was also applied to samples collected from the field. Despite its inability to resolve the close affinity species of Culex quinquefasciatus and Culex pipiens molestus, pertinent biochips are expected to be applied to a mass screening method.
authors.affiliation: International Travel Healthcare Center, 131 Dongjie Rd., Gulou District, Fuzhou, Fujian 350001, China (wangxi0620@163.com; henj98@126.com ).|Beneficial Insects Institute, Fujian Agriculture and Forestry University, 15 Shang Xia Dian Rd., Cangshan District, Fuzhou, Fujian 350002, China (jhchen34@163.com).|Department of Entomology, National Chung Hsing University, 250 Kuo-Kuan Rd., South District, Taichung 40227, Taiwan ( wctu@dragon.nchu.edu.tw; alisa120330@hotmail.com; wbyeh@nchu.edu.tw ).
authors.forename: Xi
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Entomology, National Chung Hsing University, 250 Kuo-Kuan Rd., South District, Taichung 40227, Taiwan (wctu@dragon.nchu.edu.tw; alisa120330@hotmail.com; wbyeh@nchu.edu.tw).
authors.forename: Wu-Chun
authors.initials: WC
authors.lastname: Tu
authors.affiliation: International Travel Healthcare Center, 131 Dongjie Rd., Gulou District, Fuzhou, Fujian 350001, China (wangxi0620@163.com; henj98@126.com).
authors.forename: En-Jiong
authors.initials: EJ
authors.lastname: Huang
authors.affiliation: Department of Entomology, National Chung Hsing University, 250 Kuo-Kuan Rd., South District, Taichung 40227, Taiwan (wctu@dragon.nchu.edu.tw; alisa120330@hotmail.com; wbyeh@nchu.edu.tw).
authors.forename: Yen-Hou
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Beneficial Insects Institute, Fujian Agriculture and Forestry University, 15 Shang Xia Dian Rd., Cangshan District, Fuzhou, Fujian 350002, China (jhchen34@163.com).
authors.forename: Jia-Hua
authors.initials: JH
authors.lastname: Chen
authors.affiliation: Department of Entomology, National Chung Hsing University, 250 Kuo-Kuan Rd., South District, Taichung 40227, Taiwan (wctu@dragon.nchu.edu.tw; alisa120330@hotmail.com; wbyeh@nchu.edu.tw).
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Yeh
mesh_terms: D000818:Animals; D009033:Culicidae / Q000145:classification* / Q000235:genetics / Q000378:metabolism; D021903:DNA, Ribosomal Spacer / Q000235:genetics* / Q000378:metabolism; D003576:Electron Transport Complex IV / Q000235:genetics* / Q000378:metabolism; D005260:Female; D019476:Insect Proteins / Q000235:genetics* / Q000378:metabolism; D007303:Insect Vectors / Q000145:classification* / Q000235:genetics; D008297:Male; D008928:Mitochondria / Q000235:genetics* / Q000378:metabolism; D009245:NADH Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D013045:Species Specificity
chemical_list: D021903:DNA, Ribosomal Spacer; D019476:Insect Proteins; D009245:NADH Dehydrogenase; D003576:Electron Transport Complex IV
keywords: COI; DNA chip; ITS2; mosquito