title: Establishment of Lactobacillus plantarum strain in honey bee digestive tract monitored using gfp fluorescence.
abstract: Lactic acid bacteria are symbiotic bacteria that naturally reside in the gastrointestinal tract of honey bees. They serve a multitude of functions and are considered beneficial and completely harmless. In our experiments Lactobacillus plantarum strain B35, isolated from honey bee digestive tract, was modified using pAD43-25 plasmid carrying a functional GFP gene sequence (gfpmut3a) and used as a model for monitoring and optimisation of the mode of application. The establishment of this strain in honey bee digestive tract was monitored using GFP fluorescence. Three different modes of oral application of this strain were tested: water suspension of lyophilised bacteria, aerosol application of these bacteria and consumption of sugar honey paste containing the lyophilised lactobacilli. Two days after administration the L. plantarum B35-gfp was present throughout the honey bee digestive tract with 104-105cfu/bee with highest count observed for aerosol application.
authors.affiliation: 1 Institute of Animal Physiology, Slovak Academy of Science, Šoltésovej 4-6, 040 01, Košice, Slovakia.
authors.forename: P
authors.initials: P
authors.lastname: Javorský
authors.affiliation: 1 Institute of Animal Physiology, Slovak Academy of Science, Šoltésovej 4-6, 040 01, Košice, Slovakia.
authors.forename: L Kolesár
authors.initials: LK
authors.lastname: Fecskeová
authors.affiliation: 2 Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University, Šrobárová 2, 041 80 Košice, Slovakia.
authors.forename: L
authors.initials: L
authors.lastname: Hrehová
authors.affiliation: 3 Department of Pharmacy, Pharmacology and Toxicology, University of Veterinary Medicine, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: R
authors.initials: R
authors.lastname: Sabo
authors.affiliation: 3 Department of Pharmacy, Pharmacology and Toxicology, University of Veterinary Medicine, Komenského 73, 041 81 Košice, Slovakia.
authors.forename: J
authors.initials: J
authors.lastname: Legáth
authors.affiliation: 1 Institute of Animal Physiology, Slovak Academy of Science, Šoltésovej 4-6, 040 01, Košice, Slovakia.|2 Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University, Šrobárová 2, 041 80 Košice, Slovakia.
authors.forename: P
authors.initials: P
authors.lastname: Pristas
mesh_terms: D000818:Animals; D001516:Bees / Q000382:microbiology*; D005453:Fluorescence; D000069196:Gastrointestinal Microbiome / Q000502:physiology; D041981:Gastrointestinal Tract / Q000382:microbiology*; D049452:Green Fluorescent Proteins / Q000235:genetics*; D048191:Lactiplantibacillus plantarum / Q000235:genetics* / Q000254:growth & development* / Q000302:isolation & purification; D010957:Plasmids / Q000235:genetics; D013559:Symbiosis / Q000502:physiology
chemical_list: D049452:Green Fluorescent Proteins
keywords: Lactobacillus plantarum; green fluorescent protein; honey bee digestive tract; monitoring