title: Immune oppression array elucidating immune escape and survival mechanisms in uveal melanoma.
abstract: OBJECTIVE
  To examine the genetic profile of primary uveal melanoma (UM) as compared to UM in immune escape.
  
  
  METHODS
  Dendritic cells (DC) loaded with lysates of UM cells of high metastatic potential were used to stimulate CTLs(CTLs). When CTLs co-cultured with the UM cells, most UM cells could be eliminated. Survival UM cells grew slowly and were considered to be survival variants and examined by a microarray analysis. These differential genes were analyzed further with Venn Diagrams and functions related to immune escape. We additionally examined transcriptional changes of manually selected survival variants of UM cells and of clinical UM samples by quantitative real-time polymerase chain reaction (qRT-PCR), and analyzed the correlation of these expressions and patients' survival.
  
  
  RESULTS
  Gene expression analyses revealed a marked up-regulation ofSLAMF7andCCL22and a significant down-regulation ofKRT10,FXYD3andABCC2. The expression of these genes in the relapsed UM was significantly greater than those in primary UM. UM patients with overexpression of these genes had a shorter survival period as compared with those of their underexpression.
  
  
  CONCLUSIONS
  Gene expression, in particular ofSLAMF7, CCL22, KRT10, FXYD3andABCC2, differed between primary UM cells and survival variants of UM cells.
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Lab, Beijing 100730, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Hou
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Lab, Beijing 100730, China.
authors.forename: Qi-Ming
authors.initials: QM
authors.lastname: Huang
authors.affiliation: Departments of Ophthalmology and Pathology, New York Eye and Ear Infirmary of Mount Sinai, 310 E.14th St., NY 10003, USA.
authors.forename: Dan-Ning
authors.initials: DN
authors.lastname: Hu
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Lab, Beijing 100730, China; Department of Ophthalmology, Medical Faculty Mannheim of the Ruprecht-Karls-University, Heidelberg 67117, Germany.
authors.forename: Jost B
authors.initials: JB
authors.lastname: Jonas
authors.affiliation: Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Ophthalmology and Visual Science Key Lab, Beijing 100730, China.
authors.forename: Wen-Bin
authors.initials: WB
authors.lastname: Wei
keywords: SLAMF7; dendritic cells; gene expression profile; immune escape; uveal melanoma