title: Shugan Xiaozhi Decoction Attenuates Nonalcoholic Steatohepatitis by Enhancing PPARαand L-FABP Expressions in High-Fat-Fed Rats.
abstract: This study aimed to investigate the effects of Shugan Xiaozhi decoction (SX) on nonalcoholic steatohepatitis (NASH) induced by high-fat diet in rats. The rats were randomly divided into 6 groups, namely, control, model, fenofibrate, and three different dosage of SX (10, 20, and 40 g/kg/day, p.o.). After establishing the NASH model, at 8 weeks of the experiment, treatments were administrated intragastrically to the fenofibrate and SX groups. All rats were killed after 4 weeks of treatment. Compared with the model group, alanine aminotransferase (ALT), aspartate aminotransferase (AST), free fatty acid (FFA), total cholesterol (TC), triacylglycerol (TG), and low-density lipoprotein cholesterol (LDL) serum in the serum were significantly reduced in all SX treatment groups in a dose-dependent manner. Evidence showed that SX could protect the liver by upregulating the gene and protein expressions of peroxisome proliferator-activated receptor alpha (PPARα) and liver fatty acid binding protein (L-FABP) in a dose-dependent manner. Chemical constituents of SX were further analyzed by ultraperformance liquid chromatography coupled with electrospray ionization mass spectrometry (UPLC-ESI-MS) and 30 chemicals in the ethanolic extract were tentatively identified. To conclude, our results clearly indicated that SX could protect liver functions and relieve hepatic steatosis and inflammation.
authors.affiliation: Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Hong Kong.
authors.forename: Yu-Feng
authors.identifier: 0000-0001-6762-3788
authors.initials: YF
authors.lastname: Xing
authors.affiliation: Guangzhou University of Chinese Medicine, Guangzhou 510000, Hong Kong.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Hong Kong.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Fu
authors.affiliation: Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Hong Kong.
authors.forename: Da-Qiao
authors.identifier: 0000-0002-7961-6718
authors.initials: DQ
authors.lastname: Zhou
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen 518057, Hong Kong; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Ailsa Chui-Ying
authors.identifier: 0000-0001-6740-2440
authors.initials: AC
authors.lastname: Yuen
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen 518057, Hong Kong; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Daniel Kam-Wah
authors.identifier: 0000-0002-6677-2949
authors.initials: DK
authors.lastname: Mok
authors.affiliation: State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), Shenzhen 518057, Hong Kong; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
authors.forename: Chi-On
authors.identifier: 0000-0002-5166-4814
authors.initials: CO
authors.lastname: Chan
authors.affiliation: Department of Hepatology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, Hong Kong.
authors.forename: Guang-Dong
authors.identifier: 0000-0002-8577-574X
authors.initials: GD
authors.lastname: Tong