title: Antibacterial effect of antibiotic-loaded SBA-15 on biofilm formation by Staphylococcus aureus and Staphylococcus epidermidis.
abstract: Staphylococcus aureus and Staphylococcus epidermidis are human pathogens involved in implant-related infections. During those diseases, they are able to form biofilms showing resistance to the effect of many different antibiotics. Drug delivery systems allow a local and effective delivery of antibiotics at high concentrations in the infected tissue without causing the cytotoxic effects commonly linked to systemic administration. We report the use of a porous ceramic biomaterial, such as SBA-15 loaded with antibiotics, to deliver them directly to the infected tissue. SBA-15 discs were loaded with Vancomycin, Rifampin and a combination of both, introduced in a suspension of S. aureus 15981 and S. epidermidis ATCC 35984 and incubated during 6 and 24 h. A statistically significant decrease in the biofilm density and the number of viable bacteria was detected for all antibiotics at 6 h in both bacteria. Rifampin showed an increase in the biofilm density and the number of viable bacteria at 24 h. No differences were detected between Vancomycin and the combination of antibiotics. S. epidermidis was more sensitive to the effect of the antibiotics than S. aureus. Here we have demonstrated that SBA-15 is able to act as an effective drug delivery system not only from a pharmaceutical point of view, but also from a biological one.
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Anna
authors.initials: A
authors.lastname: Aguilar-Colomer
authors.affiliation: Department of Inorganic and Bioinorganic Chemical of Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Juan Carlos
authors.initials: JC
authors.lastname: Doadrio
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Concepción
authors.initials: C
authors.lastname: Pérez-Jorge
authors.affiliation: Department of Inorganic and Bioinorganic Chemical of Universidad Complutense de Madrid, Madrid, Spain.
authors.forename: Miguel
authors.initials: M
authors.lastname: Manzano
authors.affiliation: Department of Inorganic and Bioinorganic Chemical of Universidad Complutense de Madrid, Madrid, Spain.|Networking Research Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain.
authors.forename: Maria
authors.initials: M
authors.lastname: Vallet-Regí
authors.affiliation: Department of Clinical Microbiology, IIS-Fundación Jiménez Díaz, Madrid, Spain.
authors.forename: Jaime
authors.identifier: http://orcid.org/0000-0002-8971-3167
authors.initials: J
authors.lastname: Esteban
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage / Q000494:pharmacology*; D058491:Bacterial Load; D001672:Biocompatible Materials; D018441:Biofilms / Q000187:drug effects; D002516:Ceramics; D004338:Drug Combinations; D016503:Drug Delivery Systems; D008826:Microbial Sensitivity Tests; D012293:Rifampin / Q000008:administration & dosage / Q000494:pharmacology; D012822:Silicon Dioxide / Q000008:administration & dosage / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects* / Q000502:physiology; D013212:Staphylococcus epidermidis / Q000187:drug effects* / Q000502:physiology; D014640:Vancomycin / Q000008:administration & dosage / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D004338:Drug Combinations; C509969:SBA-15; D014640:Vancomycin; D012822:Silicon Dioxide; D012293:Rifampin