title: The response of growth and patulin production of postharvest pathogen Penicillium expansum to exogenous potassium phosphite treatment.
abstract: In this study, the effects of exogenous potassium phosphite (Phi) on growth and patulin production of postharvest pathogen Penicillium expansum were assessed. The results indicated that P. expansum under 5mmol/L Phi stress presented obvious development retardation, yield reduction of patulin and lower infectivity to apple fruit. Meanwhile, expression analysis of 15 genes related to patulin biosynthesis suggested that Phi mainly affected the early steps of patulin synthetic route at transcriptional level. Furthermore, a global view of proteome and transcriptome alteration of P. expansum spores during 6h of Phi stress was evaluated by iTRAQ (isobaric tags for relative and absolute quantitation) and RNA-seq (RNA sequencing) approaches. A total of 582 differentially expressed proteins (DEPs) and 177 differentially expressed genes (DEGs) were acquired, most of which participated in carbohydrate metabolism, amino acid metabolism, lipid metabolism, genetic information processing and biosynthesis of secondary metabolites. Finally, 39 overlapped candidates were screened out through correlational analysis between iTRAQ and RNA-seq datasets. These findings will afford more precise and directional clues to explore the inhibitory mechanism of Phi on growth and patulin biosynthesis of P. expansum, and be beneficial to develop effective controlling approaches based on Phi.
authors.affiliation: Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China; Research Centre for Plant RNA Signaling, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Tongfei
authors.initials: T
authors.lastname: Lai
authors.affiliation: Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100093, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Yaya
authors.initials: Y
authors.lastname: Fan
authors.affiliation: Research Centre for Plant RNA Signaling, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Bao
authors.affiliation: Key Laboratory for Quality and Safety of Agricultural Products of Hangzhou City, College of Life and Environmental Science, Hangzhou Normal University, Hangzhou 310036, China. Electronic address: zt20100061@163.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhou
mesh_terms: D001483:Base Sequence; D004203:Disinfection / Q000379:methods*; D005511:Food Handling; D005516:Food Microbiology; D005638:Fruit / Q000382:microbiology; D005659:Fungicides, Industrial / Q000494:pharmacology*; D027845:Malus / Q000382:microbiology; D010365:Patulin / Q000096:biosynthesis*; D010407:Penicillium / Q000235:genetics / Q000254:growth & development* / Q000378:metabolism*; D017905:Phosphites / Q000494:pharmacology*; D017680:Potassium Compounds / Q000494:pharmacology*; D020543:Proteome / Q000032:analysis; D017423:Sequence Analysis, RNA
chemical_list: D005659:Fungicides, Industrial; D017905:Phosphites; D017680:Potassium Compounds; D020543:Proteome; C056591:potassium phosphite; D010365:Patulin
keywords: Patulin; Penicillium expansum; Potassium phosphite; RNA sequencing; iTRAQ