title: The regulatory effects of autophagy to the CNE2 cells radio-sensitization.
abstract: To investigate the effects of autophagy activator and autophagy inhibitor on the CNE2 radiation sensitivity of nasopharyngeal carcinoma cells. RNA interference technology was used to silence the atg5 gene and autophagy inhibition cell model was constructed. Rapamycin and chloroquine were treated respectively on cells with X-ray 5Gy irradiation. Cells' growth status were observed for 8 days and control group was set. The cell viability was detected by MTT assay and colony formation assay, and the cell cycle was analyzed by flow cytometry. Compared with the control group, the survival rate, clone formation rate and the survival rate of the irradiation of the other three groups were significantly lower. (P&lt;0.05) Most cells were detected in the G0/G1 phase in the other three groups except the control group, and cells of the other two periods were less than those in the G0/G1 phase. The autophagy inhibitor or activator and atg5 silencing can be increased by CNE2 radiation therapy, however, the sensitization effect increase of autophagy activator is better than others.
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: B-X
authors.initials: BX
authors.lastname: Li
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: X-F
authors.initials: XF
authors.lastname: Cao
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: C-Y
authors.initials: CY
authors.lastname: Weng
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: X-S
authors.initials: XS
authors.lastname: Fang
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: H-B
authors.initials: HB
authors.lastname: Mao
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: M-M
authors.initials: MM
authors.lastname: Guan
authors.affiliation: Department of Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, China.
authors.forename: G-L
authors.initials: GL
authors.lastname: Liu
mesh_terms: D001343:Autophagy / Q000187:drug effects / Q000528:radiation effects*; D000071187:Autophagy-Related Protein 5 / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D002738:Chloroquine / Q000633:toxicity; D005434:Flow Cytometry; D059585:G1 Phase Cell Cycle Checkpoints / Q000187:drug effects / Q000528:radiation effects; D005720:Gamma Rays; D006801:Humans; D034622:RNA Interference; D034741:RNA, Small Interfering / Q000378:metabolism; D011836:Radiation Tolerance / Q000187:drug effects / Q000528:radiation effects*; D020123:Sirolimus / Q000633:toxicity
chemical_list: C502778:ATG5 protein, human; D000071187:Autophagy-Related Protein 5; D034741:RNA, Small Interfering; D002738:Chloroquine; D020123:Sirolimus