title: Formaldehyde-Induced Aggravation of Pruritus and Dermatitis Is Associated with the Elevated Expression of Th1 Cytokines in a Rat Model of Atopic Dermatitis.
abstract: Atopic dermatitis is a complex disease of heterogeneous pathogenesis, in particular, genetic predisposition, environmental triggers, and their interactions. Indoor air pollution, increasing with urbanization, plays a role as environmental risk factor in the development of AD. However, we still lack a detailed picture of the role of air pollution in the development of the disease. Here, we examined the effect of formaldehyde (FA) exposure on the manifestation of atopic dermatitis and the underlying molecular mechanism in naive rats and in a rat model of atopic dermatitis (AD) produced by neonatal capsaicin treatment. The AD and naive rats were exposed to 0.8 ppm FA, 1.2 ppm FA, or fresh air (Air) for 6 weeks (2 hours/day and 5 days/week). So, six groups, namely the 1.2 FA-AD, 0.8 FA-AD, Air-AD, 1.2 FA-naive, 0.8 FA-naive and Air-naive groups, were established. Pruritus and dermatitis, two major symptoms of atopic dermatitis, were evaluated every week for 6 weeks. After that, samples of the blood, the skin and the thymus were collected from the 1.2 FA-AD, the Air-AD, the 1.2 FA-naive and the Air-naive groups. Serum IgE levels were quantified with ELISA, and mRNA expression levels of inflammatory cytokines from extracts of the skin and the thymus were calculated with qRT-PCR. The dermatitis and pruritus significantly worsened in 1.2 FA-AD group, but not in 0.8 FA-AD, compared to the Air-AD animals, whereas FA didn't induce any symptoms in naive rats. Consistently, the levels of serum IgE were significantly higher in 1.2 FA-AD than in air-AD, however, there was no significant difference following FA exposure in naive animals. In the skin, mRNA expression levels of Th1 cytokines such as TNF-α and IL-1β were significantly higher in the 1.2 FA-AD rats compared to the air-AD rats, whereas mRNA expression levels of Th2 cytokines (IL-4, IL-5, IL-13), IL-17A and TSLP were significantly higher in 1.2 FA-naive group than in the Air-naive group. These results suggested that 1.2 ppm of FA penetrated the injured skin barrier, and exacerbated Th1 responses and serum IgE level in the AD rats so that dermatitis and pruritus were aggravated, while the elevated expression of Th2 cytokines by 1.2 ppm of FA in naive rats was probably insufficient for clinical manifestation. In conclusion, in a rat model of atopic dermatitis, exposure to 1.2 ppm of FA aggravated pruritus and skin inflammation, which was associated with the elevated expression of Th1 cytokines.
authors.affiliation: Neuroscience Research Institute & Department of Physiology, Korea University College of Medicine, Seoul, Korea.
authors.forename: Rafael Taeho
authors.identifier: http://orcid.org/0000-0002-0127-2396
authors.initials: RT
authors.lastname: Han
authors.affiliation: Department of Pharmaceutics and Biotechnology, College of Medical Engineering, Konyang University, Chungnam, Korea.
authors.forename: Seung Keun
authors.initials: SK
authors.lastname: Back
authors.affiliation: Neuroscience Research Institute & Department of Physiology, Korea University College of Medicine, Seoul, Korea.
authors.forename: Hyunkyoung
authors.initials: H
authors.lastname: Lee
authors.affiliation: Neuroscience Research Institute & Department of Physiology, Korea University College of Medicine, Seoul, Korea.
authors.forename: JaeHee
authors.initials: J
authors.lastname: Lee
authors.affiliation: Neuroscience Research Institute & Department of Physiology, Korea University College of Medicine, Seoul, Korea.
authors.forename: Hye Young
authors.initials: HY
authors.lastname: Kim
authors.affiliation: Division of Biological Science and Technology, Science and Technology College, Yonsei University Wonju Campus, Wonju, Korea.
authors.forename: Hee Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: Neuroscience Research Institute & Department of Physiology, Korea University College of Medicine, Seoul, Korea.
authors.forename: Heung Sik
authors.initials: HS
authors.lastname: Na
mesh_terms: D000818:Animals; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D004195:Disease Models, Animal; D004202:Disinfectants / Q000633:toxicity; D005557:Formaldehyde / Q000009:adverse effects* / Q000633:toxicity*; D007073:Immunoglobulin E / Q000097:blood; D008297:Male; D011537:Pruritus / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012130:Respiratory Hypersensitivity; D018417:Th1 Cells / Q000378:metabolism*
chemical_list: D016207:Cytokines; D004202:Disinfectants; D005557:Formaldehyde; D007073:Immunoglobulin E