title: Genetic characterization and phylogenic analysis of H5N1 avian influenza virus detected in peafowl in Kirkuk province, Iraq.
abstract: A highly pathogenic avian influenza (HPAI), H5N1, was detected for the first time in peafowls in Kirkuk province, Iraq in 2015. Genetic analysis of the Kirkuk H5N1 indicated molecular markers for avian-type receptors. The Kirkuk H5N1 hemagglutinin gene had an infrequent amino acid cleavage site (SPQREKRRKRGLF), and neuraminidase genes showed sensitive molecular markers for antiviral drugs. Additionally, the phylogenetic analysis found that the Kirkuk H5N1 belonged to subclade 2.3.2.1c. Our results showed that the 2015 H5N1 from the Iraqi city of Kirkuk exhibited new genetic characterization and was different from the 2006 H5N1 isolate from Iraq.
authors.affiliation: Molecular Diagnostic Laboratory, Slemani Veterinary Directorate, As Sulaymaniyah, Kurdistan region, Iraq.
authors.forename: Peshnyar M A
authors.identifier: 0000-0003-3291-3405
authors.initials: PMA
authors.lastname: Rashid
authors.affiliation: Basic Sciences Department, College of Veterinary Medicine, University of Sulaimani, As Sulaymaniyah, Kurdistan region, Iraq.
authors.forename: Nahla M
authors.initials: NM
authors.lastname: Saeed
authors.affiliation: Basic Sciences Department, College of Veterinary Medicine, University of Sulaimani, As Sulaymaniyah, Kurdistan region, Iraq.
authors.forename: Hiewa Othman
authors.initials: HO
authors.lastname: Dyary
mesh_terms: D000818:Animals; D004196:Disease Outbreaks; D014644:Genetic Variation; D019267:Hemagglutinin Glycoproteins, Influenza Virus / Q000235:genetics; D053124:Influenza A Virus, H5N1 Subtype / Q000145:classification* / Q000235:genetics* / Q000302:isolation & purification* / Q000472:pathogenicity; D005585:Influenza in Birds / Q000453:epidemiology / Q000821:virology*; D007493:Iraq / Q000453:epidemiology; D009439:Neuraminidase / Q000235:genetics; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011200:Poultry / Q000821:virology*; D012367:RNA, Viral
chemical_list: D019267:Hemagglutinin Glycoproteins, Influenza Virus; D012367:RNA, Viral; D009439:Neuraminidase
keywords: H5N1; Iraq; highly-pathogenic avian influenza