title: The presence of T cell epitopes is important for induction of antibody responses against antigens directed to DEC205+dendritic cells.
abstract: In vivo antigen targeting to dendritic cells (DCs) has been used as a way to improve immune responses. Targeting is accomplished with the use of monoclonal antibodies (mAbs) to receptors present on the DC surface fused with the antigen of interest. An anti-DEC205 mAb has been successfully used to target antigens to the DEC205+CD8α+DC subset. The administration of low doses of the hybrid mAb together with DC maturation stimuli is able to activate specific T cells and induce production of high antibody titres for a number of different antigens. However, it is still not known if this approach would work with any fused protein. Here we genetically fused the αDEC205 mAb with two fragments (42-kDa and 19-kDa) derived from the ~200 kDa Plasmodium vivax merozoite surface protein 1 (MSP1), known as MSP142and MSP119, respectively. The administration of two doses of αDEC-MSP142, but not of αDEC-MSP119mAb, together with an adjuvant to two mouse strains induced high anti-MSP119antibody titres that were dependent on CD4+T cells elicited by peptides present in the MSP133sequence, indicating that the presence of T cell epitopes in antigens targeted to DEC205+DCs increases antibody responses.
authors.affiliation: Laboratory of Antigen Targeting to Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Kelly N S
authors.initials: KN
authors.lastname: Amorim
authors.affiliation: Laboratory of Antigen Targeting to Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Eline V
authors.initials: EV
authors.lastname: Rampazo
authors.affiliation: Laboratory of Antigen Targeting to Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Renan
authors.initials: R
authors.lastname: Antonialli
authors.affiliation: Laboratory of Antigen Targeting to Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, 05508-000, Brazil.
authors.forename: Marcio M
authors.initials: MM
authors.lastname: Yamamoto
authors.affiliation: CTCMol, Federal University of São Paulo, São Paulo, 04044-010, Brazil.|National Institute for Science and Technology in Vaccines, Belo Horizonte, 31270-910, Brazil.
authors.forename: Mauricio M
authors.initials: MM
authors.lastname: Rodrigues
authors.affiliation: National Institute for Science and Technology in Vaccines, Belo Horizonte, 31270-910, Brazil.|Department of Clinical and Toxicological Analysis, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, 05508-900, Brazil.
authors.forename: Irene S
authors.initials: IS
authors.lastname: Soares
authors.affiliation: Laboratory of Antigen Targeting to Dendritic Cells, Department of Parasitology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, 05508-000, Brazil.|National Institute for Science and Technology in Vaccines, Belo Horizonte, 31270-910, Brazil.
authors.forename: Silvia B
authors.initials: SB
authors.lastname: Boscardin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000911:Antibodies, Monoclonal / Q000235:genetics / Q000276:immunology / Q000378:metabolism; D000917:Antibody Formation / Q000502:physiology*; D015704:CD4 Antigens / Q000172:deficiency / Q000235:genetics; D015496:CD4-Positive T-Lymphocytes / Q000166:cytology / Q000276:immunology / Q000378:metabolism; D049109:Cell Proliferation; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology* / Q000378:metabolism; D004797:Enzyme-Linked Immunosorbent Assay; D018984:Epitopes, T-Lymphocyte / Q000235:genetics / Q000276:immunology* / Q000378:metabolism; D005260:Female; D007074:Immunoglobulin G / Q000276:immunology / Q000378:metabolism; D007371:Interferon-gamma / Q000378:metabolism; D007376:Interleukin-2 / Q000378:metabolism; D037181:Lectins, C-Type / Q000276:immunology*; D020066:Merozoite Surface Protein 1 / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000276:immunology; D013154:Spleen / Q000166:cytology / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; D015704:CD4 Antigens; D018984:Epitopes, T-Lymphocyte; D007074:Immunoglobulin G; D007376:Interleukin-2; D037181:Lectins, C-Type; D020066:Merozoite Surface Protein 1; D011993:Recombinant Fusion Proteins; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma