title: Correlation between TRAIL and caspase-8 expression and their relationship with cell proliferation and apoptosis in human osteosarcoma.
abstract: Osteosarcoma is a common malignant bone tumor that mainly affects children and adolescents. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a member of the tumor necrosis factor superfamily. Caspase-8 appears in the upstream of apoptosis signaling pathway among caspases. We investigated TRAIL and caspase-8 levels in osteosarcoma patients to determine their correlation with cell proliferation and apoptosis. Osteosarcoma and osteochondroma patients receiving surgery in our hospital were selected. TRAIL and caspase-8 expression levels in tissue were determined by immunohistochemistry, and protein levels in cells were evaluated by western blotting. Human osteosarcoma cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The osteosarcoma and osteochondroma cell cycles and apoptosis were investigated by flow cytometry. Correlation analysis was applied to TRAIL and caspase-8 levels during cell apoptosis. Positive TRAIL and caspase-8 expression rates in osteosarcoma tissue were significantly lower than in the controls (P < 0.05). TRAIL (0.114 ± 0.002) and caspase-8 (0.352 ± 0.124) levels in experimental cells were obviously lower than in the controls (P < 0.05). Osteosarcoma cells in the experimental group demonstrated higher proliferation and lower apoptosis at 24, 48, and 72 h (P < 0.05). The experimental cell number increased in the G1 stage and decreased in the S stage (P < 0.05). TRAIL and caspase-8 proteins showed positive correlation with apoptosis in osteosarcoma (P < 0.05). Human osteosarcoma presented reduced TRAIL and caspase-8 levels with enhanced cell proliferation and reduced apoptosis. TRAIL and caspase-8 expression levels were positively correlated with apoptosis in osteosarcoma.
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China xuning221@sina.com.
authors.forename: X
authors.initials: X
authors.lastname: Ning
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: X W
authors.initials: XW
authors.lastname: Shang
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: Y
authors.initials: Y
authors.lastname: Zhuang
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: M
authors.initials: M
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: H
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, The Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
authors.forename: M Z
authors.initials: MZ
authors.lastname: Huang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis; D001859:Bone Neoplasms / Q000378:metabolism* / Q000601:surgery; D053181:Caspase 8 / Q000378:metabolism*; D049109:Cell Proliferation; D002470:Cell Survival; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008297:Male; D008875:Middle Aged; D015831:Osteochondroma / Q000378:metabolism* / Q000601:surgery; D012516:Osteosarcoma / Q000378:metabolism* / Q000601:surgery; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000378:metabolism*; D014407:Tumor Cells, Cultured; D055815:Young Adult
chemical_list: D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; C505530:CASP8 protein, human; D053181:Caspase 8