title: The Mammalian-Specific Protein Armcx1 Regulates Mitochondrial Transport during Axon Regeneration.
abstract: Mitochondrial transport is crucial for neuronal and axonal physiology. However, whether and how it impacts neuronal injury responses, such as neuronal survival and axon regeneration, remain largely unknown. In an established mouse model with robust axon regeneration, we show that Armcx1, a mammalian-specific gene encoding a mitochondria-localized protein, is upregulated after axotomy in this high regeneration condition. Armcx1 overexpression enhances mitochondrial transport in adult retinal ganglion cells (RGCs). Importantly, Armcx1 also promotes both neuronal survival and axon regeneration after injury, and these effects depend on its mitochondrial localization. Furthermore, Armcx1 knockdown undermines both neuronal survival and axon regeneration in the high regenerative capacity model, further supporting a key role of Armcx1 in regulating neuronal injury responses in the adult central nervous system (CNS). Our findings suggest that Armcx1 controls mitochondrial transport during neuronal repair.
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: romain.cartoni@childrens.harvard.edu.
authors.forename: Romain
authors.initials: R
authors.lastname: Cartoni
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Michael W
authors.initials: MW
authors.lastname: Norsworthy
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Fengfeng
authors.initials: F
authors.lastname: Bei
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Chen
authors.initials: C
authors.lastname: Wang
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Siwei
authors.initials: S
authors.lastname: Li
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: Yiling
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Physiology and Biophysics, Photonics Center, Boston University School of Medicine, Boston, MA 02118, USA.
authors.forename: Christopher V
authors.initials: CV
authors.lastname: Gabel
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA; Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: thomas.schwarz@childrens.harvard.edu.
authors.forename: Thomas L
authors.initials: TL
authors.lastname: Schwarz
authors.affiliation: F.M. Kirby Neurobiology Center, Boston Children's Hospital, 300 Longwood Avenue, Boston, MA 02115, USA; Department of Neurology, Harvard Medical School, Boston, MA 02115, USA. Electronic address: zhigang.he@childrens.harvard.edu.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: He
mesh_terms: D000818:Animals; D051186:Armadillo Domain Proteins / Q000235:genetics* / Q000378:metabolism; D001369:Axons / Q000378:metabolism* / Q000648:ultrastructure; D019771:Axotomy; D001692:Biological Transport; D002540:Cerebral Cortex / Q000166:cytology; D004195:Disease Models, Animal; D055785:Gene Knockdown Techniques; D007150:Immunohistochemistry; D017403:In Situ Hybridization; D051379:Mice; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism* / Q000648:ultrastructure; D024101:Mitochondrial Proteins / Q000235:genetics* / Q000378:metabolism; D009416:Nerve Regeneration / Q000235:genetics*; D009474:Neurons / Q000378:metabolism; D009900:Optic Nerve / Q000378:metabolism* / Q000648:ultrastructure; D020221:Optic Nerve Injuries / Q000235:genetics*; D012038:Regeneration; D012160:Retina; D012165:Retinal Ganglion Cells / Q000378:metabolism* / Q000648:ultrastructure; D059008:Time-Lapse Imaging
chemical_list: D051186:Armadillo Domain Proteins; C000621382:Armcx1 protein, mouse; D024101:Mitochondrial Proteins
keywords: axonal regeneration; axonal transport; mitochondria; optic nerve; retinal ganglion cells