title: Increased Leptin and Leptin Receptor Expression in Dogs With Gallbladder Mucocele.
abstract: BACKGROUND
  Leptin and its receptor play a role in several disease processes such as pancreatitis and heart disease. However, their association with gallbladder mucocele (GBM) in dogs has not been reported.
  
  
  OBJECTIVE
  To evaluate differences in the expression of leptin and leptin receptor between dogs with and without GBM.
  
  
  METHODS
  Twenty-five healthy dogs, including 9 laboratory beagle dogs, and 22 client-owned dogs with GBM.
  
  
  METHODS
  Serum leptin concentration was determined in blood samples of all dogs by ELISA. Canine gallbladder samples were collected from 9 dogs with GBM that underwent surgery for therapeutic purposes and from 9 healthy laboratory beagle dogs as a normal control group. Samples were analyzed for leptin and leptin receptor mRNA by real-time polymerase chain reaction.
  
  
  RESULTS
  Serum leptin concentration was significantly higher in dogs with GBM than in healthy dogs (medians of 7.03 and 2.18 ng/mL, respectively; P < .001). Patients with GBM that had undergone surgery had significantly higher serum leptin concentrations than those that had not (medians of 12.2 and 4.09 ng/mL, respectively; P = .001). However, no difference in serum leptin concentration was found between dogs with GBM with or without endocrinopathies. The mRNA expression levels of leptin and its receptor were significantly increased in the gallbladder tissues of dogs with GBM.
  
  
  CONCLUSIONS
  Dysregulation of leptin might be involved in the pathophysiology of GBM, and leptin concentrations might be associated with GBM severity.
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Korea.
authors.forename: S
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Korea.
authors.forename: O-K
authors.initials: OK
authors.lastname: Kweon
authors.affiliation: Department of Veterinary Clinical Sciences, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Gwanak-gu, Seoul, Korea.
authors.forename: W H
authors.initials: WH
authors.lastname: Kim
mesh_terms: D000818:Animals; D002769:Cholelithiasis / Q000378:metabolism / Q000662:veterinary*; D004283:Dog Diseases / Q000097:blood / Q000378:metabolism*; D004285:Dogs; D004797:Enzyme-Linked Immunosorbent Assay / Q000662:veterinary; D005260:Female; D005704:Gallbladder / Q000378:metabolism*; D020738:Leptin / Q000097:blood / Q000378:metabolism*; D008297:Male; D010375:Pedigree; D054411:Receptors, Leptin / Q000097:blood / Q000378:metabolism*
chemical_list: D020738:Leptin; D054411:Receptors, Leptin
keywords: Canine; Gallbladder mucocele; Leptin; Leptin receptor