title: [Effect of WT1 Gene High Expression and CD34+and Auer+on Prognosis of Acute Myeloid Leukemia].
abstract: OBJECTIVE
  To explore the clinical significance of WT1 expression level in patient with AML non-M3,understand the biological characteristics of Auer+and CD34+AML and its effects on first induced remission rate and prognosis.
  
  
  METHODS
  The RQ-PCR was used to detect the WT1 expression levels in 92 patients suffering AML non-M3; the relationship between the CR1 and OS was analysed and the differences of CR1 and OS in AML with Auer+and Auer-,CD34+and CD34-were compared.
  
  
  RESULTS
  AML with WT1 high expression level at first visit had quite lower CR1 (P<0.05). AML with CD34+had quite lower CR1 (P<0.05). Compared with Auer-patients, CR1 of Auer+CML patients was higher (P<0.05), the OS of AML patients with WT1 high expression level was lower than that of the AML patients with low expression level of WT1, and with significant differences (P<0.05), the OS of AML patients with CD34+was lower than that of AML patients with CD34-, and with significant differences (P<0.05). There was no obvious difference in OS between the AML patients with Auer+and Auer-.
  
  
  CONCLUSIONS
  High expression level of WT1 and CD34+in AML patients suggests the poor prognosis. The Auer positive only relats with CR1, and does not relate with OS.
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Anhui Medical University, Hefei 230000, Anhui Province, China.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Hu
authors.affiliation: Department of Immunology, College of Basic Medical Sciences, Anhui Medical University, Hefei 230000, Anhui Province, China. E-mail:592914198@qq.com.
authors.forename: Bao-Jun
authors.initials: BJ
authors.lastname: Huang
authors.affiliation: Department of Laboratorial Medicine, Bengbu Medical College, Bengbu 233000 Anhui Province, China.
authors.forename: Shao-Jun
authors.initials: SJ
authors.lastname: Pan
mesh_terms: D018952:Antigens, CD34; D015870:Gene Expression; D006801:Humans; D015470:Leukemia, Myeloid, Acute; D011379:Prognosis; D012074:Remission Induction; D025721:WT1 Proteins
chemical_list: D018952:Antigens, CD34; D025721:WT1 Proteins; C583751:WT1 protein, human