title: Intra and inter species genetic variability of transferrin receptor gene regions in Trypanosoma evansi isolates of different livestock and geographical regions of India.
abstract: Trypanosoma evansi, the aetiological agent of Surra affects a wide range of livestock and wild animals in India. In the present study, we studied intra- and inter species genetic variability in the transferrin receptor encoding gene regions (ESAG6/7 gene region) of T. evansi isolates by cloning, sequencing and phylogenetic study collected from camel, cattle, donkeys and ponies from North-Western and Central India. The nucleotide sequence variation of ESAG6/7 gene region between Indian T. evansi isolates was up to 17.7% and amino acid sequence variation was up to 31%. Twenty nine clones from six T. evansi isolates from geographical regions of India were included into Clade 1, 5, 6, 7 and 9 consisting of ESAG6 variants reported among T. evansi isolates from South-east Asia and South America. The cladogram indicated a relation between the host species and the genetic variability in the hyper-variable region of ESAG6 gene. Analysis of the Indian ESAG6 variants and their respective Clade positions presented a host specific distribution indicating homogenous parasite population in their respective animal hosts.
authors.forename: Souti Prasad
authors.initials: SP
authors.lastname: Sarkhel
authors.forename: S K
authors.initials: SK
authors.lastname: Gupta
authors.forename: Jyoti
authors.initials: J
authors.lastname: Kaushik
authors.forename: Jarnail
authors.initials: J
authors.lastname: Singh
authors.forename: Vijesh Kumar
authors.initials: VK
authors.lastname: Saini
authors.forename: Sanjay
authors.initials: S
authors.lastname: Kumar
authors.forename: Rajender
authors.initials: R
authors.lastname: Kumar
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005786:Gene Expression Regulation / Q000502:physiology; D014644:Genetic Variation; D007194:India / Q000453:epidemiology; D058751:Livestock; D051379:Mice; D010802:Phylogeny; D011990:Receptors, Transferrin / Q000235:genetics / Q000378:metabolism*; D013045:Species Specificity; D014345:Trypanosoma / Q000235:genetics / Q000378:metabolism*; D014352:Trypanosomiasis / Q000453:epidemiology / Q000469:parasitology / Q000662:veterinary*
chemical_list: D011990:Receptors, Transferrin