title: Development of a high-density genetic linkage map and identification of flowering time QTLs in adzuki bean (Vigna angularis).
abstract: A high-density linkage map is crucial for the identification of quantitative trait loci (QTLs), positional cloning, and physical map assembly. Here, we report the development of a high-density linkage map based on specific length amplified fragment sequencing (SLAF-seq) for adzuki bean and the identification of flowering time-related QTLs. Through SLAF library construction and Illumina sequencing of a recombinant inbred line (RIL) population, a total of 4425 SLAF markers were developed and assigned to 11 linkage groups (LGs). After binning the SLAF markers that represented the same genotype, the final linkage map of 1628.15 cM contained 2032 markers, with an average marker density of 0.80 cM. Comparative analysis showed high collinearity with two adzuki bean physical maps and a high degree of synteny with the reference genome of common bean (Phaseolus vulgaris). Using this map, one major QTL on LG03 and two minor QTLs on LG05 associated with first flowering time (FLD) were consistently identified in tests over a two-year period. These results provide a foundation that will be useful for future genomic research, such as identifying QTLs for other important traits, positional cloning, and comparative mapping in legumes.
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China.
authors.forename: Changyou
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China.
authors.forename: Baojie
authors.initials: B
authors.lastname: Fan
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Cao
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China.
authors.forename: Qiuzhu
authors.initials: Q
authors.lastname: Su
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China.
authors.forename: Zhixiao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Crop Genetics and Breeding of Hebei Province; Institute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Shijiazhuang 050035, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tian
mesh_terms: D002874:Chromosome Mapping / Q000379:methods*; D035264:Flowers / Q000502:physiology*; D015723:Gene Library; D017343:Genes, Plant; D008040:Genetic Linkage; D005819:Genetic Markers; D018745:Genome, Plant; D005838:Genotype; D027805:Phaseolus / Q000235:genetics; D010641:Phenotype; D040641:Quantitative Trait Loci; D017422:Sequence Analysis, DNA; D026801:Synteny; D000070658:Vigna / Q000235:genetics* / Q000502:physiology
chemical_list: D005819:Genetic Markers