title: Short hairpin RNA directed against β-catenin inhibits prostate cancer growth and invasion in vitro.
abstract: β-catenin protein exhibits a dual function in epithelial cells, depending on its intracellular localization. At the plasma membrane, β‑catenin is an important constituent of adherens junctions. However, when the Wnt/β‑catenin signaling pathway is activated, β‑catenin translocates to the nucleus to promote speciﬁc gene expression. To investigate the functional activity and examine the role of the Wnt/β‑catenin signaling pathway in various human prostate cancer cells, indirect immunofluorescence was performed to detect the expression and distribution of β‑catenin in the following prostate cancer cell lines: PC‑3, LNCaP, C4‑2, IA8‑ARCaP and IF11‑ARCaP. A marked difference was observed in the expression and distribution of β‑catenin in different prostate cancer cell lines. β‑catenin was observed in the nuclei of IA8-ARCaP and IF11‑ARCaP cell lines, whereas it was present on the membrane of LNCaP and C4‑2 cell lines. There was a low expression of β‑catenin in the PC‑3 cell line. Furthermore, short hairpin RNA (shRNA) targeting human β‑catenin was constructed to investigate the effect of β‑catenin shRNA on the proliferation and invasive potency of prostate cancer cells. The IA8/β‑catenin(‑) cell line exhibited a reduced potency for invasion and proliferation compared with the IA8 and IA8‑shControl groups. The present study demonstrated that suppressing activity of Wnt/β‑catenin signal pathway via β‑catenin shRNA results in an inhibition of prostate cancer proliferation and invasion.
authors.affiliation: Department of Urology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Respiratory and Critical Care Medicine, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.
authors.forename: Chunting
authors.initials: C
authors.lastname: Wu
authors.affiliation: Department of Urology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Urology, Beijing Anzhen Hospital, Capital Medical University, Beijing 100029, P.R. China.
authors.forename: Yongguang
authors.initials: Y
authors.lastname: Jiang
mesh_terms: D045744:Cell Line, Tumor; D002465:Cell Movement; D049109:Cell Proliferation; D006801:Humans; D008297:Male; D008856:Microscopy, Fluorescence; D011471:Prostatic Neoplasms / Q000378:metabolism / Q000473:pathology; D034622:RNA Interference; D012333:RNA, Messenger / Q000378:metabolism; D034741:RNA, Small Interfering / Q000378:metabolism*; D060449:Wnt Signaling Pathway; D051176:beta Catenin / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*
chemical_list: D012333:RNA, Messenger; D034741:RNA, Small Interfering; D051176:beta Catenin