title: Assessment of the carcinogenic potential of high intense-sweeteners through the test for detection of epithelial tumor clones (warts) in Drosophila melanogaster.
abstract: High intensity-sweeteners (HIS) are natural or synthetic substances, sweeter than sugar, providing sweetness without calories. Sweeteners are mainly used as an aid in losing weight, preventing obesity and controlling blood sugar levels for diabetics. The objective of this study was to evaluate the carcinogenic potential of the sweeteners aspartame, sucralose, sodium saccharin and steviol glycoside, using the test for detection of epithelial tumor clones in Drosophila melanogaster. Larvae of 72 ± 4h, obtained from wts/TM3 female mated with mwh/mwh males, were treated for approximately 48h with different concentrations of aspartame (0.85, 1.7, 3.4, 6.8 or 13.6 mM ); sucralose (0.5, 1.25, 2.5, 5.0 or 10 mM); sodium saccharin (25; 50; 100; 200 or 400 mM) and steviol glycoside (2.5; 5.0; 10; 20 or 40 mM). Water (Reverse Osmosis) and doxorubicin (DXR 0.4 mM) were used as negative and positive controls, respectively. No statistically significant differences were observed (p > 0.05) in tumor frequencies in individuals treated with all concentrations of these sweeteners when compared to negative control. It was therefore concluded that, in these experimental conditions, aspartame, sucralose, sodium saccharin and steviol glycoside have no carcinogenic effect in D. melanogaster.
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Campus Umuarama, Uberlândia, Minas Gerais, Brazil; Centro Universitário de Patos de Minas, Laboratório de Citogenética e Mutagênese, Patos de Minas, Minas Gerais, Brazil.
authors.forename: Mirley Alves
authors.initials: MA
authors.lastname: Vasconcelos
authors.affiliation: Centro Universitário de Patos de Minas, Laboratório de Citogenética e Mutagênese, Patos de Minas, Minas Gerais, Brazil.
authors.forename: Priscila Capelari
authors.initials: PC
authors.lastname: Orsolin
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Campus Umuarama, Uberlândia, Minas Gerais, Brazil; Centro Universitário de Patos de Minas, Laboratório de Citogenética e Mutagênese, Patos de Minas, Minas Gerais, Brazil.
authors.forename: Rosiane Gomes
authors.initials: RG
authors.lastname: Silva-Oliveira
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Campus Umuarama, Uberlândia, Minas Gerais, Brazil; Centro Universitário de Patos de Minas, Laboratório de Citogenética e Mutagênese, Patos de Minas, Minas Gerais, Brazil.
authors.forename: Júlio César
authors.initials: JC
authors.lastname: Nepomuceno
authors.affiliation: Universidade Federal de Uberlândia, Instituto de Genética e Bioquímica, Campus Umuarama, Uberlândia, Minas Gerais, Brazil. Electronic address: maspano@ufu.br.
authors.forename: Mário Antônio
authors.initials: MA
authors.lastname: Spanó
mesh_terms: D000818:Animals; D063646:Carcinogenesis / Q000187:drug effects*; D002273:Carcinogens / Q000633:toxicity*; D004331:Drosophila melanogaster / Q000187:drug effects*; D005260:Female; D007814:Larva / Q000187:drug effects / Q000254:growth & development; D008297:Male; D064306:Non-Nutritive Sweeteners / Q000633:toxicity*; D009995:Osmosis / Q000187:drug effects; D018675:Toxicity Tests / Q000379:methods*; D014860:Warts / Q000175:diagnosis* / Q000188:drug therapy / Q000473:pathology
chemical_list: D002273:Carcinogens; D064306:Non-Nutritive Sweeteners
keywords: Aspartame; High Intensity-Sweeteners; Sodium saccharin; Steviol glycoside; Sucralose; Warts