title: Assay of superoxide dismutase activity in a plate assay using WST-1.
abstract: One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol that minimizes sources of variability.
authors.affiliation: Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Peskin
authors.affiliation: Centre for Free Radical Research, Department of Pathology, University of Otago Christchurch, Christchurch, New Zealand. Electronic address: christine.winterbourn@otago.ac.nz.
authors.forename: Christine C
authors.initials: CC
authors.lastname: Winterbourn
mesh_terms: D000818:Animals; D002417:Cattle; D057075:Enzyme Assays / Q000295:instrumentation / Q000379:methods*; D007700:Kinetics; D010084:Oxidation-Reduction; D015203:Reproducibility of Results; D012996:Solutions; D013482:Superoxide Dismutase / Q000737:chemistry*; D013778:Tetrazolium Salts / Q000737:chemistry*
chemical_list: C110656:2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium; D012996:Solutions; D013778:Tetrazolium Salts; D013482:Superoxide Dismutase
keywords: Assay; Superoxide dismutase; Tetrazolium; WST-1