title: Modeling Acinetobacter baumannii wound infections: The critical role of iron.
abstract: Acinetobacter baumannii has emerged as an increasingly important and successful opportunistic human pathogen due to its ability to withstand harsh environmental conditions, its characteristic virulence factors, and quick adaptability to stress.
  
  
  
  We developed a clinically relevant murine model of A. baumannii traumatic wound infection to determine the effect of local wound environment on A. baumannii virulence. Mice underwent rectus muscle crush injury combined with ischemia created by epigastric vessel ligation, followed by A. baumannii inoculation. Reiterative experiments were performed using (1) a mutant deficient in the production of the siderophore acinetobactin, or (2) iron supplementation of the wound milieu. Mice were euthanized 7 days later, and rectus muscle analyzed for signs of clinical infection, HIF1α accumulation, bacterial abundance, and colony morphotype. To determine the effect of wound milieu on bacterial virulence, Galleria mellonella infection model was used.
  
  
  
  The combination of rectus muscle injury with ischemia and A. baumannii inoculation resulted in 100% incidence of clinical wound infection that was significantly higher compared with other groups (n = 15/group, p < 0.0001). The highest level of wound infection was accompanied by the highest level of A. baumannii colonization (p < 0.0001) and the highest degree of HIF1α accumulation (p < 0.05). A. baumannii strains isolated from injured/ischemic muscle with clinical infection displayed a rough morphotype and a higher degree of virulence as judged by G. mellonella killing assay as compared with smooth morphotype colonies isolated from injured muscle without clinical infection (100% vs. 60%, n = 30 Log-Rank test, p = 0.0422). Iron supplementation prevented wound infection (n = 30, p < 0.0001) and decreased HIF1α (p = 0.039643). Similar results of decrease in wound infection and HIF1α were obtained when A. baumannii wild type was replaced with its derivative mutant [INCREMENT]BasD deficient in acinetobactin production.
  
  
  
  The ability of A. baumannii to cause infections in traumatized wound relies on its ability to scavenge iron and can be prevented by iron supplementation to the wound milieu.
authors.affiliation: From the Center for Surgical Infection Research and Therapeutics (I.D.F., M.A.K., N.B., A.Z., J.D., L.C., O.Z., J.C.A.), University of Chicago Pritzker School of Medicine, Chicago, Illinois; and Department of Microbiology (L.A.A.), Miami University, Oxford, Ohio.
authors.forename: Irma D
authors.initials: ID
authors.lastname: Fleming
authors.forename: Monika A
authors.initials: MA
authors.lastname: Krezalek
authors.forename: Natalia
authors.initials: N
authors.lastname: Belogortseva
authors.forename: Alexander
authors.initials: A
authors.lastname: Zaborin
authors.forename: Jennifer
authors.initials: J
authors.lastname: Defazio
authors.forename: Laxmipradha
authors.initials: L
authors.lastname: Chandrasekar
authors.forename: Luis A
authors.initials: LA
authors.lastname: Actis
authors.forename: Olga
authors.initials: O
authors.lastname: Zaborina
authors.forename: John C
authors.initials: JC
authors.lastname: Alverdy
mesh_terms: D000007:Abdominal Injuries / Q000188:drug therapy / Q000382:microbiology; D000151:Acinetobacter Infections / Q000188:drug therapy*; D040981:Acinetobacter baumannii / Q000472:pathogenicity*; D000818:Animals; D015153:Blotting, Western; D004195:Disease Models, Animal; D007501:Iron / Q000494:pharmacology*; D051379:Mice; D008810:Mice, Inbred C57BL; D009036:Moths; D017568:Rectus Abdominis / Q000098:blood supply / Q000293:injuries; D014774:Virulence; D037521:Virulence Factors; D014946:Wound Infection / Q000188:drug therapy* / Q000382:microbiology*
chemical_list: D037521:Virulence Factors; D007501:Iron