title: Influence of chitosan-chitin nanofiber composites on cytoskeleton structure and the proliferation of rat bone marrow stromal cells.
abstract: Chitosan scaffolds have gained much attention in various tissue engineering applications, but the effect of their microstructure on cell-material spatial interactions remains unclear. Our objective was to evaluate the effect of chitosan-based matrices doping with chitin nano-whiskers (CNW) on adhesion, spreading, cytoskeleton structure, and proliferation of rat bone marrow stromal cells (BMSCs). The behavior of BMSCs during culture on chitosan-CNW films was determined by the molecular mass, hydrophobicity, porosity, crosslinking degree, protonation degree and molecular structure of the composite chitosan-CNW films. The shape, spreading area, cytoskeleton structure, and proliferation of BMSCs on chitosan matrices with a crystalline structure and high porosity were similar to that observed for BMSCs cultured on polystyrene tissue culture plates. The amorphous polymer structure and high swelling led to a decrease in the spreading area and cell proliferation. Thus, we can control the behavior of cells in culture (adhesion, spreading, and proliferation) by changing the physico-chemical properties of the chitosan-CNW films.
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Pereyaslavskaya ul. 23, Kharkov, 61015, Ukraine.
authors.forename: Victoria V
authors.initials: VV
authors.lastname: Kiroshka
authors.affiliation: Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg, 199004, Russian Federation.
authors.forename: Valentina A
authors.initials: VA
authors.lastname: Petrova
authors.affiliation: Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg, 199004, Russian Federation.
authors.forename: Daniil D
authors.initials: DD
authors.lastname: Chernyakov
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Pereyaslavskaya ul. 23, Kharkov, 61015, Ukraine.
authors.forename: Yulia O
authors.initials: YO
authors.lastname: Bozhkova
authors.affiliation: Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Pereyaslavskaya ul. 23, Kharkov, 61015, Ukraine.
authors.forename: Katerina V
authors.initials: KV
authors.lastname: Kiroshka
authors.affiliation: Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg, 199004, Russian Federation.
authors.forename: Yulia G
authors.initials: YG
authors.lastname: Baklagina
authors.affiliation: Institute of Silicate Chemistry of the Russian Academy of Sciences, Adm. Makarova nab. 2, St. Petersburg, 199034, Russian Federation.
authors.forename: Dmitry P
authors.initials: DP
authors.lastname: Romanov
authors.affiliation: Institute of Chemistry, St. Petersburg State University, Universitetskii pr. 26, Petrodvorets, St. Petersburg, 198504, Russian Federation.
authors.forename: Roman V
authors.initials: RV
authors.lastname: Kremnev
authors.affiliation: Institute of Macromolecular Compounds of the Russian Academy of Sciences, Bolshoi pr. VO 31, St. Petersburg, 199004, Russian Federation. yury_skorik@mail.ru.
authors.forename: Yury A
authors.initials: YA
authors.lastname: Skorik
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry; D049109:Cell Proliferation; D002686:Chitin / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003460:Crystallization; D003599:Cytoskeleton / Q000737:chemistry*; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D057139:Nanofibers / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D016062:Porosity; D051381:Rats; D017208:Rats, Wistar; D012212:Rheology; D023822:Tissue Engineering / Q000379:methods; D054457:Tissue Scaffolds / Q000737:chemistry; D014783:Viscosity; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011108:Polymers; D011137:Polystyrenes; D002686:Chitin; D048271:Chitosan