title: Functional characterisation of the YIPF protein family in mammalian cells.
abstract: In this study, we carry out a systematic characterisation of the YIPF family of proteins with respect to their subcellular localisation profile, membrane topology and functional effects on the endomembrane system. YIPF proteins primarily localise to the Golgi complex and can be grouped into trans-Golgi-localising YIPFs (YIPF1 and YIPF2) and cis-Golgi-localising YIPFs (YIPF3, YIPF4 and YIPF5), with YIPF6 and YIPF7 showing a broader profile being distributed throughout the Golgi stack. YIPF proteins have a long soluble N-terminal region, which is orientated towards the cytosol, followed by 5 closely stacked transmembrane domains, and a C terminus, orientated towards the lumen of the Golgi. The significance of YIPF proteins for the maintenance of the morphology of the Golgi was tested by RNA interference, revealing a number of specific morphological changes to this organelle on their depletion. We propose a role for this family of proteins in regulating membrane dynamics in the endomembrane system.
authors.affiliation: School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.|UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland.|Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Aškerčeva 7, 1000, Ljubljana, Slovenia.
authors.forename: Tilen
authors.initials: T
authors.lastname: Kranjc
authors.affiliation: School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland.|UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland.
authors.forename: Eugene
authors.initials: E
authors.lastname: Dempsey
authors.affiliation: UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland.|School of Biomolecular and Biomedical Science, University College Dublin, Dublin 4, Ireland.
authors.forename: Gerard
authors.initials: G
authors.lastname: Cagney
authors.affiliation: Department of Molecular Biosciences, Faculty of Life Sciences, Kyoto Sangyo University, Motoyama, Kamigamo, Kita, Kyoto, 603-8555, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nakamura
authors.affiliation: UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland.|School of Medicine and Medical Science, University College Dublin, Dublin 4, Ireland.
authors.forename: Denis C
authors.initials: DC
authors.lastname: Shields
authors.affiliation: School of Biology and Environmental Science, University College Dublin, Dublin 4, Ireland. jeremy.simpson@ucd.ie.|UCD Conway Institute of Biomolecular and Biomedical Research, University College Dublin, Dublin 4, Ireland. jeremy.simpson@ucd.ie.
authors.forename: Jeremy C
authors.identifier: http://orcid.org/0000-0002-7956-7805
authors.initials: JC
authors.lastname: Simpson
mesh_terms: D006056:Golgi Apparatus / Q000378:metabolism; D006367:HeLa Cells; D006801:Humans; D008565:Membrane Proteins / Q000378:metabolism*; D014407:Tumor Cells, Cultured
chemical_list: D008565:Membrane Proteins; C000619311:YIPF2 protein, human
keywords: Golgi apparatus; Membrane traffic; Subcellular localisation; Transmembrane proteins; YIPF