title: Ciprofloxacin-Mediated Mutagenesis Is Suppressed by Subinhibitory Concentrations of Amikacin in Pseudomonas aeruginosa.
abstract: Resistance to antibiotics is a global health problem. Activation of the SOS response, and the subsequent elevation in mutagenesis, contributes to the appearance of resistance mutations. Among currently used drugs, quinolones are the most potent inducers of the SOS response. In the present study, we show that amikacin inhibits ciprofloxacin-mediated SOS induction and mutagenesis inPseudomonas aeruginosa.
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Estela Ynés
authors.initials: EY
authors.lastname: Valencia
authors.affiliation: Department of Clinical Analysis, School of Pharmacy, University of São Paulo, São Paulo, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Esposito
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.
authors.forename: Beny
authors.initials: B
authors.lastname: Spira
authors.affiliation: Instituto de Biomedicina de Sevilla (IBIS)-CSIC, Seville, Spain.|Centro Nacional de Biotecnología-CSIC, Madrid, Spain.
authors.forename: Jesús
authors.identifier: 0000-0003-0495-3848
authors.initials: J
authors.lastname: Blázquez
authors.affiliation: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil rgalhard@usp.br.
authors.forename: Rodrigo S
authors.identifier: 0000-0002-5686-9704
authors.initials: RS
authors.lastname: Galhardo
mesh_terms: D000583:Amikacin / Q000494:pharmacology*; D000900:Anti-Bacterial Agents / Q000494:pharmacology; D002939:Ciprofloxacin / Q000037:antagonists & inhibitors / Q000494:pharmacology*; D052978:Disk Diffusion Antimicrobial Tests; D015870:Gene Expression; D017930:Genes, Reporter; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D016296:Mutagenesis / Q000187:drug effects; D009154:Mutation; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011550:Pseudomonas aeruginosa / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development; D011938:Rec A Recombinases / Q000235:genetics* / Q000378:metabolism; D013014:SOS Response, Genetics / Q000187:drug effects
chemical_list: D000900:Anti-Bacterial Agents; D002939:Ciprofloxacin; D000583:Amikacin; D008156:Luciferases; D011938:Rec A Recombinases
keywords: Pseudomonas aeruginosa; SOS response; ciprofloxacin; recA