title: Actinotalea caeni sp. nov., isolated from a sludge sample of a biofilm reactor.
abstract: A polyphasic taxonomic study was carried out on strain EBR-4-2T isolated from a biofilm reactor in Korea. Cells of the strain were Gram-stain-positive, non-spore-forming, non-motile and rod-shaped. Comparative 16S rRNA gene sequence studies showed the clear affiliation of this strain to the Actinobacteria, and it had the highest pairwise sequence similarities with Actinotalea suaedae EGI 60002T (98.7 %), Actinotalea ferrariae CF5-4T (96.3 %) and Actinotalea fermentans DSM 3133T (96.2 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that the strain formed a clear phylogenetic lineage with the genus Actinotalea. The major fatty acids were identified as C15 : 0 anteiso, C16 : 0, C16 : 0 N alcohol, C15 : 1 anteiso A and C15 : 0 iso. The major cellular polar lipids were diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol and glycolipid. The peptidoglycan type was A4β containing l-Orn-d-Glu. The whole-cell-wall sugars were glucose and ribose. The respiratory quinone was identified as menaquinone MK-10(H4), and the genomic DNA G+C content was determined to be 74.8 mol %. Based on evidence from this polyphasic study, it is proposed that strain EBR-4-2T should be designated as representing a novel species named Actinotalea caeni sp. nov. The type stain is EBR-4-2T (=KCTC 33604T=JCM 30447T).
authors.affiliation: College of Biology and the Environment, Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210-037, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Jin
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
authors.forename: So-Ra
authors.initials: SR
authors.lastname: Ko
authors.affiliation: Culture Collection Team, Freshwater Bioresources Culture Research Division, Nakdonggang National Institute of Biological Resources, Sangju 37242, Republic of Korea.
authors.forename: Chang Soo
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
authors.forename: Chi-Yong
authors.initials: CY
authors.lastname: Ahn
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeollabuk-do 56212, Republic of Korea.
authors.forename: Jung-Sook
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, 181 Ipsin-gil, Jeollabuk-do 56212, Republic of Korea.
authors.forename: Keun Chul
authors.initials: KC
authors.lastname: Lee
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
authors.forename: Hee-Mock
authors.initials: HM
authors.lastname: Oh
authors.affiliation: Cell Factory Research Centre, Korea Research Institute of Bioscience & Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
authors.forename: Hyung-Gwan
authors.initials: HG
authors.lastname: Lee
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D018441:Biofilms; D019149:Bioreactors / Q000382:microbiology*; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D006017:Glycolipids / Q000737:chemistry; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D056910:Republic of Korea; D017422:Sequence Analysis, DNA; D012722:Sewage / Q000382:microbiology*; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D006017:Glycolipids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D012722:Sewage; D014451:Ubiquinone; C025204:Ubiquinone Q2