title: Manual resuscitators in successive use in the same patient: reservoir of multi- and extensively resistant bacteria.
abstract: To determine the bacterial load on manual resuscitators (MRs), swab samples of MR connectors from a Brazilian intensive care unit were collected at time zero, and after 24 and 48h use in the same patient. From 20 MRs, a total of 54 bacteria were isolated: six at t=0, 17 at t=24, and 31 at t=48. Staphylococcus aureus, Acinetobacter baumannii, Serratia marcescens, and Pseudomonas aeruginosa were the predominant isolates, and multidrug resistance was widespread in all species. Greater awareness of the need to ensure effective decontamination of MRs between patients, as well as more frequent replacement of MRs in individual patients, is recommended to minimize the risk of (re)colonization/infection of the respiratory tract.
authors.affiliation: Faculty of Nursing, Federal University of Goias, Goiânia, Brazil. Electronic address: gipinheirolima@hotmail.com.
authors.forename: G
authors.initials: G
authors.lastname: Pinheiro Lima Aires Gomes
authors.affiliation: Faculty of Nursing, Federal University of Goias, Goiânia, Brazil; School of Social and Health Sciences, Pontifical Catholic University of Goias, Goiânia, Brazil.
authors.forename: A
authors.initials: A
authors.lastname: Custódia Silva E Souza
authors.affiliation: Institute of Tropical Pathology and Public Health, Federal University of Goias, Goiânia, Brazil.
authors.forename: L S
authors.initials: LS
authors.lastname: Netto de Oliveira Leão-Vasconcelos
authors.affiliation: Faculty of Nursing, Federal University of Goias, Goiânia, Brazil; Faculty of Medicine and Health Sciences, Macquarie University, Australia.
authors.forename: D
authors.initials: D
authors.lastname: de Melo Costa
authors.affiliation: Clinics Hospital, Federal University of Goias, Goiânia, Brazil.
authors.forename: S
authors.initials: S
authors.lastname: Bisinoto Alves
authors.affiliation: Faculty of Nursing, Federal University of Goias, Goiânia, Brazil.
authors.forename: H
authors.initials: H
authors.lastname: Carneiro Cunha Neves
authors.affiliation: Faculty of Nursing, Federal University of Goias, Goiânia, Brazil; School of Social and Health Sciences, Pontifical Catholic University of Goias, Goiânia, Brazil.
authors.forename: M
authors.initials: M
authors.lastname: Severino Pereira
mesh_terms: D001419:Bacteria / Q000145:classification / Q000187:drug effects / Q000302:isolation & purification*; D058491:Bacterial Load; D001938:Brazil; D024901:Drug Resistance, Multiple, Bacterial; D004864:Equipment and Supplies / Q000382:microbiology*; D006801:Humans; D007362:Intensive Care Units; D011446:Prospective Studies; D012151:Resuscitation / Q000295:instrumentation*
keywords: Equipment contamination; Hospital infection; Intensive care units; Multidrug resistance