title: Tempol effect on epithelial-mesenchymal transition induced by hyperglycemia.
abstract: BACKGROUND
  One of common mechanisms in pathophysiology of chronic kidney diseases is epithelial-mesenchymal transition (EMT). On the other hand oxidative stress has been known to participate in kidney damage of diabetic nephropathy (DN).
  
  
  OBJECTIVE
  We studied if tempol, a well-known antioxidant agent, can ameliorate EMT in DN induced in male rats.
  
  
  METHODS
  Twenty-seven male rats were equally divided in to 4 groups. Group I (control or C), group II (diabetic or D), group III (T) rats which was given tempol (100 mg/kg/day) by gavages for 28 days and group IV (D&T) was diabetic rats that also received same dose of tempol. After treatment, their kidneys were studied by immunohistochemicalstaining.
  
  
  RESULTS
  Pathological changes in the kidney were detected concurrently with increasing kidney weight and urinary albumin excretion. In addition, EMT indices, i.e. decline of E-cadherin and increase of α SMA staining were significantly emerged in the renal tubular cells of diabetic group and were partially modified in diabetic group which were simultaneously treated by tempol.
  
  
  CONCLUSIONS
  Tempol can modify, but not significantly, EMT induced by DN.
authors.affiliation: Pathology ward, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Jafari
authors.affiliation: Nephrology Ward, Department of Internal Medicine, Iran University of medical Sciences, Tehran, Iran.
authors.forename: Farahnaz
authors.initials: F
authors.lastname: Dadras
authors.affiliation: Pathology ward, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Hamid Reza
authors.initials: HR
authors.lastname: Ghadimipour
authors.affiliation: Department of Community Medicine, Hamdan University of Medical Sciences, Hamadan, Iran.
authors.forename: Mohamad Ali
authors.initials: MA
authors.lastname: Seif Rabiei
authors.affiliation: Urology and Nephrology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Khoshjou
keywords: Diabetic nephropathy; Epithelial mesenchymal transition; Tempol