title: Current state and perspectives in modeling and control of human pluripotent stem cell expansion processes in stirred-tank bioreactors.
abstract: Implementation of model-based practices for process development, control, automation, standardization, and validation are important factors for therapeutic and industrial applications of human pluripotent stem cells. As robust cultivation strategies for pluripotent stem cell expansion and differentiation have yet to be determined, process development could be enhanced by application of mathematical models and advanced control systems to optimize growth conditions. Therefore, it is important to understand both the potential of possible applications and the apparent limitations of existing mathematical models to improve pluripotent stem cell cultivation technologies. In the present review, the authors focus on these issues as they apply to stem cell expansion processes. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:355-364, 2017.
authors.affiliation: Department of Automation, Kaunas University of Technology, Kaunas, Lithuania.
authors.forename: Vytautas
authors.initials: V
authors.lastname: Galvanauskas
authors.affiliation: Department of Automation, Kaunas University of Technology, Kaunas, Lithuania.
authors.forename: Vykantas
authors.initials: V
authors.lastname: Grincas
authors.affiliation: Department of Automation, Kaunas University of Technology, Kaunas, Lithuania.
authors.forename: Rimvydas
authors.initials: R
authors.lastname: Simutis
authors.affiliation: Department of Biotechnology, Osaka University, Osaka, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kagawa
authors.affiliation: Department of Biotechnology, Osaka University, Osaka, Japan.
authors.forename: Masahiro
authors.initials: M
authors.lastname: Kino-Oka
mesh_terms: D061249:Batch Cell Culture Techniques / Q000379:methods / Q000639:trends*; D019149:Bioreactors; D002454:Cell Differentiation / Q000502:physiology; D049109:Cell Proliferation / Q000502:physiology; D000066673:Cell Self Renewal / Q000502:physiology*; D002470:Cell Survival / Q000502:physiology; D002478:Cells, Cultured; D003198:Computer Simulation; D025461:Feedback, Physiological / Q000502:physiology*; D005544:Forecasting; D006801:Humans; D008954:Models, Biological; D039904:Pluripotent Stem Cells / Q000166:cytology* / Q000502:physiology*
keywords: control systems; mathematical models; pluripotent stem cells; stirred-tanks; three-dimensional expansion