title: A mouse model of chronic bacterial lesions (a cotton trap) for studying oral bacteria - lymphocyte interactions.
abstract: We established a mouse model of chronic bacterial infection (cotton trap) to get a deeper insight into interactions between immune cells and bacterial strains, that are most commonly isolated from periapical processes. We have used flow cytometry to identify the presence of intracellular cytokines of activated T cells collected from cotton traps, previously infected with different strains of bacteria and implanted subcutaneously into the back of the mice. We provide an evidence that anaerobic bacteria (Bacteroides sp.) and nocardiae are more effective in inducing cytotoxic immunity and Th1 response compared to oral streptococci. Differences in immune response against anaerobic bacteria when compared to streptococci are probably dependent on some non-specific immune cell stimulation (e.g. by bacterial cell wall components), nevertheless the role of specific antigen-dependent immune mechanism can not be excluded.
authors.affiliation: Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Petra
authors.initials: P
authors.lastname: Hudler
authors.affiliation: Institute for Microbiology and Immunology, Medical Faculty Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Marija
authors.initials: M
authors.lastname: Gubina
authors.affiliation: Department of Maxilofacial and Oral Surgery, University Clinical Centre Ljubljana, Zaloška 7, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Nataša Ihan
authors.initials: NI
authors.lastname: Hren
authors.affiliation: Institute for Microbiology and Immunology, Medical Faculty Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Katja
authors.initials: K
authors.lastname: Seme
authors.affiliation: Institute for Microbiology and Immunology, Medical Faculty Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Tadej
authors.initials: T
authors.lastname: Malovrh
authors.affiliation: Institute of Pathology, Medical Faculty, Zaloška 7, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Nina
authors.initials: N
authors.lastname: Gale
authors.affiliation: Institute of Microbiology and Immunology, Medical Faculty, University of Ljubljana, Zaloška 4, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Alojz
authors.initials: A
authors.lastname: Ihan
keywords: Key words oral bacteria; lymphocytes; mouse model