title: Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels.
abstract: Pyruvate ferredoxin oxidoreductase from Citrobacter sp. S-77 (PFORS77) was purified in order to develop a method for acetyl-CoA production. Although the purified PFORS77showed high O2-sensitivity, the activity could be remarkably stabilized in anaerobic conditions. PFORS77was effectively immobilized on ceramic hydroxyapatite (PFORS77-HA) with an efficiency of more than 96%, however, after encapsulation of PFORS77-HA in alginate, the rate of catalytic acetyl-CoA production was highly reduced to 36% when compared to that of the free enzyme. However, the operational stability of the PFORS77-HA in alginate hydrogels was remarkable, retaining over 68% initial activity even after ten repeated cycles. The results suggested that the PFORS77-HA hydrogels have a high potential for biotechnological application.
authors.affiliation: International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Takenaka
authors.affiliation: International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Centre for Small Molecule Energy, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Ki-Seok
authors.initials: KS
authors.lastname: Yoon
authors.affiliation: International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Centre for Small Molecule Energy, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: International Institute for Carbon-Neutral Energy Research (WPI-I(2)CNER), Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan; Centre for Small Molecule Energy, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan. Electronic address: ogo.seiji.872@m.kyushu-u.ac.jp.
authors.forename: Seiji
authors.initials: S
authors.lastname: Ogo
mesh_terms: D000105:Acetyl Coenzyme A / Q000138:chemical synthesis*; D000464:Alginates / Q000737:chemistry*; D002384:Catalysis; D002954:Citrobacter / Q000201:enzymology; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D020100:Hydrogels / Q000737:chemistry*; D050743:Pyruvate Synthase / Q000737:chemistry* / Q000378:metabolism
chemical_list: D000464:Alginates; D006603:Hexuronic Acids; D020100:Hydrogels; D000105:Acetyl Coenzyme A; D020723:Glucuronic Acid; D050743:Pyruvate Synthase
keywords: Acetyl-CoA production; Encapsulation; Immobilization; Kinetic parameters; Purification and characterization; Pyruvate ferredoxin oxidoreductase