title: Role of extracellular vesicles in the interaction between epithelial and mesenchymal cells during oviductal ciliogenesis.
abstract: Extracellular vesicles (EVs) have been shown to transport miRNA, mRNA and protein, suggesting that they are new communication mediators. Diffusible mesenchymal factors determine the fate of Műllerian epithelial cells into oviductal ciliated cells. In the present study, we investigated whether EVs mediate the communication in the epithelial-mesenchymal interaction during oviductal ciliogenesis. EVs were isolated from cells of oviductal mesenchymal cell line (S1 cells) and characterized by TEM and expression of exosomal marker CD81. CD81 protein was also detected in oviductal mesenchyme, suggesting that CD81-expressing exosomes may be secreted from oviductal mesenchyme, as well as S1 cells. β-actin, Gapdh and Vimentin mRNAs and miRNAs were detected in the exosomes. mRNA in S1 cells was able to be transported into cells of Műllerian epithelial cell line (E1 cells) via the exosomes. The effects of exosomes derived from S1 cells on ciliogenesis of E1 cells were analyzed by in vitro models. Culture with exosomes increased the number of ciliated cells in E1 cells. These results suggest that exosomes derived from mesenchymal cells modulate the oviductal ciliogenesis and open new avenues for developmental study of EVs.
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
authors.forename: Shota
authors.initials: S
authors.lastname: Nakano
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan; Graduate Program in Bioscience, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan.
authors.forename: Shohei
authors.initials: S
authors.lastname: Yamamoto
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
authors.forename: Atsumasa
authors.initials: A
authors.lastname: Okada
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan.
authors.forename: Tadaaki
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: Department of Material and Biological Science, Faculty of Science, Japan Women's University, 2-8-1 Mejirodai, Bunkyou-ku, Tokyo 112-8681, Japan.
authors.forename: Mamiko
authors.initials: M
authors.lastname: Sato
authors.affiliation: Department of Material and Biological Science, Faculty of Science, Japan Women's University, 2-8-1 Mejirodai, Bunkyou-ku, Tokyo 112-8681, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Takagi
authors.affiliation: Department of Biological Science and Technology, Faculty of Industrial Science and Technology, Tokyo University of Science, 6-3-1 Niijuku, Katsushika-ku, Tokyo 125-8585, Japan. Electronic address: tomoylab@rs.noda.tus.ac.jp.
authors.forename: Yasuhiro
authors.initials: Y
authors.lastname: Tomooka
mesh_terms: D000199:Actins / Q000235:genetics; D000818:Animals; D002923:Cilia; D004847:Epithelial Cells / Q000166:cytology* / Q000378:metabolism; D055354:Exosomes / Q000378:metabolism; D000067128:Extracellular Vesicles / Q000502:physiology*; D005187:Fallopian Tubes / Q000166:cytology*; D005260:Female; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D008815:Mice, Inbred Strains; D012333:RNA, Messenger / Q000378:metabolism; D060225:Tetraspanin 28 / Q000378:metabolism; D014746:Vimentin
chemical_list: D000199:Actins; C554799:Cd81 protein, mouse; D012333:RNA, Messenger; D060225:Tetraspanin 28; D014746:Vimentin
keywords: Ciliogenesis; Exosome; Extracellular vesicles; Oviduct