title: High-yield production of aryl alcohol oxidase under limited growth conditions in small-scale systems using a mutant Aspergillus nidulans strain.
abstract: Aryl alcohol oxidase (MtGloA) is an enzyme that belongs to the ligninolytic consortium and can play an important role in the bioenergy industry. This study investigated production of an MtGloA client enzyme by a mutant strain of Aspergillus nidulans unable to synthesize its own pyridoxine. Pyridoxine limitation can be used to control cell growth, diverting substrate to protein production. In agitated culture, enzyme production was similar when using media with 1 mg/L and without pyridoxine (26.64 ± 6.14 U/mg mycelia and 26.14 ± 8.39 U/mg mycelia using media with and without pyridoxine, respectively). However, the treatment lacking pyridoxine had to be supplemented with pyridoxine after 156 h of fermentation to sustain continued enzyme production. Use of extremely diluted pyridoxine levels allowed reduced fungal growth while maintaining steady enzyme production. Concentrations of 9 and 13.5 µg/L pyridoxine allowed MtGloA production with a growth rate of only 5% of that observed when using the standard 1 mg/L pyridoxine media.
authors.affiliation: Department of Biosystems and Agricultural Engineering, Oklahoma State University, 111 Agriculture Hall, Stillwater, OK, 74078, USA.
authors.forename: Oscar
authors.initials: O
authors.lastname: Pardo-Planas
authors.affiliation: Department of Microbiology and Molecular Genetics, Oklahoma State University, 307 Life Sciences East, Stillwater, OK, 74078, USA.
authors.forename: Rolf A
authors.initials: RA
authors.lastname: Prade
authors.affiliation: Department of Biosystems and Agricultural Engineering, Oklahoma State University, 111 Agriculture Hall, Stillwater, OK, 74078, USA. mwilkins3@unl.edu.|Industrial Agricultural Products Center, University of Nebraska-Lincoln, 211 L.W. Chase Hall, Lincoln, NE, 68583, USA. mwilkins3@unl.edu.
authors.forename: Mark R
authors.initials: MR
authors.lastname: Wilkins
mesh_terms: D000429:Alcohol Oxidoreductases / Q000096:biosynthesis*; D001233:Aspergillus nidulans / Q000201:enzymology* / Q000235:genetics; D003470:Culture Media / Q000737:chemistry; D005285:Fermentation; D006863:Hydrogen-Ion Concentration; D007218:Industrial Microbiology; D011736:Pyridoxine / Q000378:metabolism
chemical_list: D003470:Culture Media; D000429:Alcohol Oxidoreductases; C077759:aryl-alcohol oxidase; D011736:Pyridoxine
keywords: Aryl alcohol oxidase; Enzyme production; Filamentous fungi; MtGloA; Pyridoxine limitation