title: Proteome analysis of human serum proteins adsorbed onto different titanium surfaces used in dental implants.
abstract: Titanium dental implants are commonly used due to their biocompatibility and biochemical properties; blasted acid-etched Ti is used more frequently than smooth Ti surfaces. In this study, physico-chemical characterisation revealed important differences in roughness, chemical composition and hydrophilicity, but no differences were found in cellular in vitro studies (proliferation and mineralization). However, the deposition of proteins onto the implant surface might affect in vivo osseointegration. To test that hypothesis, protein layers formed on discs of both surface type after incubation with human serum were analysed. Using mass spectrometry (LC/MS/MS), 218 proteins were identified, 30 of which were associated with bone metabolism. Interestingly, Apo E, antithrombin and protein C adsorbed mostly onto blasted and acid-etched Ti, whereas the proteins of the complement system (C3) were found predominantly on smooth Ti surfaces. These results suggest that physico-chemical characteristics could be responsible for the differences observed in the adsorbed protein layer.
authors.affiliation: a Department of Industrial Systems and Design Engineering , University of Castellón , Castellón de la Plana , Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Romero-Gavilán
authors.affiliation: b Department of Medicine , University of Castellón , Castellón de la Plana , Spain.
authors.forename: N C
authors.initials: NC
authors.lastname: Gomes
authors.affiliation: a Department of Industrial Systems and Design Engineering , University of Castellón , Castellón de la Plana , Spain.
authors.forename: Joaquin
authors.initials: J
authors.lastname: Ródenas
authors.affiliation: b Department of Medicine , University of Castellón , Castellón de la Plana , Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Sánchez
authors.affiliation: c Proteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII , Derio , Spain.
authors.forename: Mikel
authors.initials: M
authors.lastname: Azkargorta
authors.affiliation: c Proteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII , Derio , Spain.
authors.forename: Ibon
authors.initials: I
authors.lastname: Iloro
authors.affiliation: c Proteomics Platform, CIC bioGUNE, CIBERehd, ProteoRed-ISCIII , Derio , Spain.
authors.forename: Felix
authors.initials: F
authors.lastname: Elortza
authors.affiliation: d Department of Polymer Science and Technology , University of Basque Country , San Sebastián , Spain.
authors.forename: Iñaki
authors.initials: I
authors.lastname: García Arnáez
authors.affiliation: d Department of Polymer Science and Technology , University of Basque Country , San Sebastián , Spain.
authors.forename: Mariló
authors.initials: M
authors.lastname: Gurruchaga
authors.affiliation: d Department of Polymer Science and Technology , University of Basque Country , San Sebastián , Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Goñi
authors.affiliation: a Department of Industrial Systems and Design Engineering , University of Castellón , Castellón de la Plana , Spain.
authors.forename: Julio
authors.initials: J
authors.lastname: Suay
mesh_terms: D000327:Adsorption; D000818:Animals; D001798:Blood Proteins / Q000032:analysis*; D015921:Dental Implants; D006801:Humans; D008855:Microscopy, Electron, Scanning; D016348:Osseointegration; D040901:Proteomics; D013499:Surface Properties; D053719:Tandem Mass Spectrometry; D014025:Titanium / Q000737:chemistry*
chemical_list: D001798:Blood Proteins; D015921:Dental Implants; D014025:Titanium
keywords: Titanium; apolipoprotein E; bone regeneration; human serum; proteomics; surface properties