title: A comprehensive approach to ascertain the binding mode of curcumin with DNA.
abstract: Curcumin is a natural phytochemical from the rhizoma of Curcuma longa, the popular Indian spice that exhibits a wide range of pharmacological properties like antioxidant, anticancer, anti-inflammatory, antitumor, and antiviral activities. In the published literatures we can see different studies and arguments on the interaction of curcumin with DNA. The intercalative binding, groove binding and no binding of curcumin with DNA were reported. In this context, we conducted a detailed study to understand the mechanism of recognition of dimethylsulfoxide-solubilized curcumin by DNA. The interaction of curcumin with calf thymus DNA (ctDNA) was confirmed by agarose gel electrophoresis. The nature of binding and energetics of interaction were studied by Isothermal Titration Calorimetry (ITC), Differential Scanning Calorimetry (DSC), UV-visible, fluorescence and melting temperature (Tm) analysis. The experimental data were compared with molecular modeling studies. Our investigation confirmed that dimethylsulfoxide-solubilized curcumin binds in the minor groove of the ctDNA without causing significant structural alteration to the DNA.
authors.affiliation: School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala 686560, India.
authors.forename: P
authors.initials: P
authors.lastname: Haris
authors.affiliation: School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala 686560, India.
authors.forename: Varughese
authors.initials: V
authors.lastname: Mary
authors.affiliation: School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala 686560, India.
authors.forename: P
authors.initials: P
authors.lastname: Aparna
authors.affiliation: Department of Biotechnology and Microbiology, Kannur University, Thalassery Campus, Palayad, Kerala 670661, India.
authors.forename: K V
authors.initials: KV
authors.lastname: Dileep
authors.affiliation: School of Pure and Applied Physics, Mahatma Gandhi University, Kottayam, Kerala 686560, India; Center for High Performance Computing, Mahatma Gandhi University, Kottayam, Kerala 686560, India. Electronic address: c.sudarsan.mgu@gmail.com.
authors.forename: C
authors.initials: C
authors.lastname: Sudarsanakumar
mesh_terms: D000818:Animals; D002151:Calorimetry; D002152:Calorimetry, Differential Scanning; D002417:Cattle; D003474:Curcumin / Q000737:chemistry / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism*; D008958:Models, Molecular; D009691:Nucleic Acid Denaturation; D013050:Spectrometry, Fluorescence; D013056:Spectrophotometry, Ultraviolet; D013696:Temperature
chemical_list: D004247:DNA; C427203:calf thymus DNA; D003474:Curcumin
keywords: Agarose gel electrophoresis; Calf thymus DNA; Curcumin; Differential Scanning Calorimetry; Isothermal Titration Calorimetry; Melting temperature analysis