title: In Situ Real-Time Monitoring of Glutamate and Electrophysiology from Cortex to Hippocampus in Mice Based on a Microelectrode Array.
abstract: Changes in the structure and function of the hippocampus contribute to epilepsy, schizophrenia and other neurological or mental disorders of the brain. Since the function of the hippocampus depends heavily on the glutamate (Glu) signaling pathways, in situ real-time detection of Glu neurotransmitter release and electrophysiological signals in hippocampus is of great significance. To achieve the dual-mode detection in mouse hippocampus in vivo, a 16-channel implantable microelectrode array (MEA) was fabricated by micro-electromechanical system (MEMS) technology. Twelve microelectrode sites were modified with platinum black for electrophysiological recording and four sites were modified with glutamate oxidase (GluOx) and 1,3-phenylenediamine (mPD) for selective electrochemical detection of Glu. The MEA was implanted from cortex to hippocampus in mouse brain for in situ real-time monitoring of Glu and electrophysiological signals. It was found that the Glu concentration in hippocampus was roughly 50 μM higher than that in the cortex, and the firing rate of concurrently recorded spikes declined from 6.32 ± 4.35 spikes/s in cortex to 0.09 ± 0.06 spikes/s in hippocampus. The present results demonstrated that the dual-mode MEA probe was capable in neurological detections in vivo with high spatial resolution and dynamical response, which lays the foundation for further pathology studies in the hippocampus of mouse models with nervous or mental disorders.
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. xinyi_fan@126.com.|University of Chinese Academy of Sciences, Beijing 100190, China. xinyi_fan@126.com.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. ylsong@mail.ie.ac.cn.|University of Chinese Academy of Sciences, Beijing 100190, China. ylsong@mail.ie.ac.cn.
authors.forename: Yilin
authors.initials: Y
authors.lastname: Song
authors.affiliation: Institute of Mental Health, the Sixth Hospital of Peking University, Beijing 100191, China. mayuanlin2010@bjmu.edu.cn.
authors.forename: Yuanlin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. zhangsong0112@126.com.|University of Chinese Academy of Sciences, Beijing 100190, China. zhangsong0112@126.com.
authors.forename: Song
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. xiaoguihua11@126.com.|University of Chinese Academy of Sciences, Beijing 100190, China. xiaoguihua11@126.com.
authors.forename: Guihua
authors.initials: G
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. lilisheepmiemie@163.com.|University of Chinese Academy of Sciences, Beijing 100190, China. lilisheepmiemie@163.com.
authors.forename: Lili
authors.initials: L
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. xuhuiren_2012@163.com.|University of Chinese Academy of Sciences, Beijing 100190, China. xuhuiren_2012@163.com.
authors.forename: Huiren
authors.initials: H
authors.lastname: Xu
authors.affiliation: Institute of Mental Health, the Sixth Hospital of Peking University, Beijing 100191, China. daizhang@bjmu.edu.cn.
authors.forename: Dai
authors.initials: D
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China. xxcai@mail.ie.ac.cn.|University of Chinese Academy of Sciences, Beijing 100190, China. xxcai@mail.ie.ac.cn.
authors.forename: Xinxia
authors.initials: X
authors.lastname: Cai
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods; D004594:Electrophysiology / Q000295:instrumentation* / Q000379:methods*; D018698:Glutamic Acid / Q000032:analysis*; D006624:Hippocampus / Q000378:metabolism; D051379:Mice; D055617:Micro-Electrical-Mechanical Systems / Q000295:instrumentation* / Q000379:methods*; D008839:Microelectrodes; D010655:Phenylenediamines / Q000032:analysis
chemical_list: D010655:Phenylenediamines; D018698:Glutamic Acid; C008381:3-phenylenediamine
keywords: MEMS; glutamate; hippocampus; implantable microelectrode array; spike