title: Detection and in vitro metabolism of the confiscated peptides BPC 157 and MGF R23H.
abstract: A new peptide, body protecting compound (BPC), BPC 157, and a variant of mechano-growth factor (MGF), MGF R23H, were identified in confiscated vials. BPC 157 has the amino acid sequence, GEPPPGKPADDAGLV, and is currently under investigation for the promotion of healing and recovery in a variety of tissues. In vitro metabolism experiments in plasma demonstrate that MGF R23H has good stability and should be detectable in urine, while BPC 157 forms a stable metabolite that should be detectable in urine. A weak cation exchange solid phase extraction method was validated for detection of BPC 157 in urine. The method has a limit of detection of 0.1 ng/mL, precision of less than 20%, and good linearity, r20.998. BPC 157 was stable in urine for at least 4 days. The specificity of the method is improved by measurement of a potential BPC metabolite along with the parent peptide. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Sports Medicine Research and Testing Laboratory, 560 Arapeen Dr., Suite 150A, Salt Lake City, UT, 84108, USA.
authors.forename: Holly D
authors.identifier: http://orcid.org/0000-0002-2345-4334
authors.initials: HD
authors.lastname: Cox
authors.affiliation: Sports Medicine Research and Testing Laboratory, 560 Arapeen Dr., Suite 150A, Salt Lake City, UT, 84108, USA.|Division of Physical Medicine and Rehabilitation, University of Utah School of Medicine, Salt Lake City, UT, 84112, USA.
authors.forename: Geoff D
authors.initials: GD
authors.lastname: Miller
authors.affiliation: Sports Medicine Research and Testing Laboratory, 560 Arapeen Dr., Suite 150A, Salt Lake City, UT, 84108, USA.|Division of Physical Medicine and Rehabilitation, University of Utah School of Medicine, Salt Lake City, UT, 84112, USA.
authors.forename: Daniel
authors.initials: D
authors.lastname: Eichner
mesh_terms: D000595:Amino Acid Sequence; D004300:Doping in Sports; D005260:Female; D006801:Humans; D007334:Insulin-Like Growth Factor I / Q000032:analysis / Q000378:metabolism / Q000652:urine*; D057230:Limit of Detection; D008297:Male; D010446:Peptide Fragments / Q000032:analysis / Q000378:metabolism / Q000652:urine*; D011506:Proteins / Q000032:analysis / Q000378:metabolism; D052616:Solid Phase Extraction / Q000379:methods; D015813:Substance Abuse Detection / Q000379:methods*
chemical_list: D010446:Peptide Fragments; D011506:Proteins; D007334:Insulin-Like Growth Factor I; C083833:BPC 157
keywords: BPC 157; MGF; mass spectrometry; peptides