title: Arsenite in drinking water produces glucose intolerance in pregnant rats and their female offspring.
abstract: Drinking water is the main source of arsenic exposure. Chronic exposure has been associated with metabolic disorders. Here we studied the effects of arsenic on glucose metabolism, in pregnant and post-partum of dams and their offspring. We administered 5 (A5) or 50 (A50) mg/L of sodium arsenite in drinking water to rats from gestational day 1 (GD1) until two months postpartum (2MPP), and to their offspring from weaning until 8 weeks old. Liver arsenic dose-dependently increased in arsenite-treated rats to levels similar to exposed population. Pregnant A50 rats gained less weight than controls and recovered normal weight at 2MPP. Arsenite-treated pregnant animals showed glucose intolerance on GD16-17, with impaired insulin secretion but normal insulin sensitivity; they showed dose-dependent increased pancreas insulin on GD18. All alterations reverted at 2MPP. Offspring from A50-treated mothers showed lower body weight at birth, 4 and 8 weeks of age, and glucose intolerance in adult females, probably due to insulin secretion and sensitivity alterations. Arsenic alters glucose homeostasis during pregnancy by altering beta-cell function, increasing risk of developing gestational diabetes. In pups, it induces low body weight from birth to 8 weeks of age, and glucose intolerance in females, demonstrating a sex specific response.
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, CABA, Argentina; Escuela de Ciencia y Técnica, Universidad Nacional de San Martín, Av 25 de mayo 1650, Partido de San Martín, Bs As, Argentina. Electronic address: mmbonaventura@gmail.com.
authors.forename: María Marta
authors.initials: MM
authors.lastname: Bonaventura
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, CABA, Argentina. Electronic address: nsbourguignon@yahoo.com.ar.
authors.forename: Nadia Soledad
authors.initials: NS
authors.lastname: Bourguignon
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, CABA, Argentina. Electronic address: m.bizzozzero@hotmail.com.
authors.forename: Marianne
authors.initials: M
authors.lastname: Bizzozzero
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, CABA, Argentina. Electronic address: keruvino@hotmail.com.
authors.forename: Diego
authors.initials: D
authors.lastname: Rodriguez
authors.affiliation: Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Viamonte 444, CABA, Argentina.
authors.forename: Clara
authors.initials: C
authors.lastname: Ventura
authors.affiliation: Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Viamonte 444, CABA, Argentina. Electronic address: cmcocca@ffyb.uba.ar.
authors.forename: Claudia
authors.initials: C
authors.lastname: Cocca
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, CABA, Argentina; Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, CABA, Argentina. Electronic address: libertun@dna.uba.ar.
authors.forename: Carlos
authors.initials: C
authors.lastname: Libertun
authors.affiliation: Instituto de Biología y Medicina Experimental (IBYME-CONICET), Vuelta de Obligado 2490, C1428ADN, CABA, Argentina. Electronic address: vlux@ibyme.conicet.gov.ar.
authors.forename: Victoria Adela
authors.initials: VA
authors.lastname: Lux-Lantos
mesh_terms: D000818:Animals; D018053:Arsenites / Q000633:toxicity*; D001835:Body Weight / Q000187:drug effects; D060766:Drinking Water / Q000009:adverse effects* / Q000032:analysis; D005260:Female; D018149:Glucose Intolerance / Q000209:etiology*; D005951:Glucose Tolerance Test; D007328:Insulin / Q000032:analysis; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000209:etiology*; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018053:Arsenites; D060766:Drinking Water; D007328:Insulin; C015001:arsenite
keywords: Arsenite; Body weight; Glucose intolerance; Offspring; Pregnant and postpartum rats