title: The microtubule end-binding protein FgEB1 regulates polar growth and fungicide sensitivity via different interactors in Fusarium graminearum.
abstract: In yeasts, the end-binding protein 1 (EB1) homologs regulate microtubule dynamics, cell polarization, and chromosome stability. However, functions of EB1 orthologs in plant pathogenic fungi have not been characterized yet. Here, we observed that the FgEB1 deletion mutant (ΔFgEB1) of Fusarium graminearum exhibits twisted hyphae, increased hyphal branching and curved conidia, indicating that FgEB1 is involved in the regulation of cellular polarity. Microscopic examination further showed that the microtubules of ΔFgEB1 exhibited less organized in comparison with those of the wild type. In addition, the lack of FgEB1 also altered the distribution of polarity-related class I myosin via the interaction with the actin. On the other hand, we identified four core septins as FgEB1-interacting proteins, and found that FgEB1 and septins regulated conidial polar growth in the opposite orientation. Interestingly, FgEB1 and FgKar9 constituted another complex that modulated the response to carbendazim, a microtubule-damaging agent specifically. In addition, the deletion of FgEB1 led to dramatically decreased deoxynivalenol (DON) biosynthesis. Taken together, results of this study indicate that FgEB1 regulates cellular polarity, fungicide sensitivity and DON biosynthesis via different interactors in F. graminarum, which provides a novel insight into understanding of the biological functions of EB1 in filamentous fungi.
authors.affiliation: Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Zunyong
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Sisi
authors.initials: S
authors.lastname: Wu
authors.affiliation: Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Xinyue
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Plant Pathology, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Gu
authors.affiliation: Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Yanni
authors.initials: Y
authors.lastname: Yin
authors.affiliation: Institute of Biotechnology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.|State Key Laboratory of Rice Biology, Zhejiang University, 866 Yuhangtang Road, Hangzhou, 310058, China.
authors.forename: Zhonghua
authors.initials: Z
authors.lastname: Ma
mesh_terms: D001562:Benzimidazoles / Q000494:pharmacology; D002219:Carbamates / Q000494:pharmacology; D002352:Carrier Proteins / Q000235:genetics / Q000378:metabolism; D016764:Cell Polarity / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D005659:Fungicides, Industrial / Q000494:pharmacology; D005670:Fusarium / Q000187:drug effects / Q000235:genetics* / Q000254:growth & development*; D017353:Gene Deletion; D025301:Hyphae / Q000254:growth & development; D008826:Microbial Sensitivity Tests; D008869:Microtubule-Associated Proteins / Q000235:genetics / Q000378:metabolism*; D008870:Microtubules / Q000235:genetics*; D009218:Myosins; D009687:Nuclear Proteins / Q000378:metabolism; D010935:Plant Diseases / Q000382:microbiology; D058112:Septins / Q000378:metabolism; D013172:Spores, Fungal / Q000254:growth & development; D014255:Trichothecenes / Q000096:biosynthesis*; D014908:Triticum / Q000382:microbiology; D014774:Virulence
chemical_list: D001562:Benzimidazoles; D002219:Carbamates; D002352:Carrier Proteins; C405804:EB1 microtubule binding proteins; D005656:Fungal Proteins; D005659:Fungicides, Industrial; D008869:Microtubule-Associated Proteins; D009687:Nuclear Proteins; D014255:Trichothecenes; D058112:Septins; D009218:Myosins; C006698:carbendazim; C007262:deoxynivalenol