title: The rs3736228 polymorphism in the LRP5 gene is associated with calcaneal ultrasound parameter but not with body composition in a cohort of young Caucasian adults.
abstract: The aim of the present study was to investigate the possible influence of low-density lipoprotein receptor-related protein 5 (LRP5) and sclerostin (SOST) genes as genetic factors contributing to calcaneal quantitative ultrasound (QUS) and body composition variables in a population of young Caucasian adults. The study population comprised a total of 575 individuals (mean age 20.41years; SD 2.36) whose bone mass was assessed through QUS to determine broadband ultrasound attenuation (BUA, dB/MHz). Body composition measurements were performed using a body composition analyser. Seven single-nucleotide polymorphisms (SNPs) of LRP5 (rs2306862, rs599083, rs556442 and rs3736228) and SOST (rs4792909, rs851054 and rs2023794) were selected as genetic markers and genotyped using TaqMan OpenArray®technology. Linear regression analysis was used to test the possible association of the tested SNPs with QUS and body composition parameters. Linear regression analysis revealed that the rs3736228 SNP of LPR5 was significantly associated with BUA after adjustment for age, sex, weight, height, physical activity and calcium intake (P = 0.028, β (95% CI) = 0.089 (0.099-1.691). For the remaining SNPs, no significant association with the QUS measurement was observed. Regarding body composition, no significant association was found between LRP5 and SOST polymorphisms and body mass index, total fat mass and total lean mass after adjustment for age and sex as covariates. We concluded that the rs3736228 LRP5 genetic polymorphism influences calcaneal QUS parameter in a population of young Caucasian adults. This finding suggests that LRP5 might be an important genetic marker contributing to bone mass accrual early in life.
authors.affiliation: Faculty of Health Sciences, University of Granada, Av. Ilustración S/N, 18007, Granada, Spain. macoro@ugr.es.
authors.forename: María
authors.initials: M
authors.lastname: Correa-Rodríguez
authors.affiliation: Faculty of Health Sciences, University of Granada, Av. Ilustración S/N, 18007, Granada, Spain.
authors.forename: Jacqueline
authors.initials: J
authors.lastname: Schmidt-RioValle
authors.affiliation: Faculty of Health Sciences, University of Granada, Av. Ilustración S/N, 18007, Granada, Spain.
authors.forename: Blanca
authors.initials: B
authors.lastname: Rueda-Medina
mesh_terms: D000328:Adult; D001823:Body Composition / Q000235:genetics*; D015992:Body Mass Index; D001835:Body Weight; D015519:Bone Density / Q000235:genetics; D002111:Calcaneus / Q000000981:diagnostic imaging*; D015331:Cohort Studies; D015444:Exercise; D005260:Female; D056726:Genetic Association Studies; D005819:Genetic Markers; D006801:Humans; D060492:Low Density Lipoprotein Receptor-Related Protein-5 / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D014463:Ultrasonography; D044465:White People / Q000235:genetics*; D055815:Young Adult
chemical_list: D005819:Genetic Markers; C555482:LRP5 protein, human; D060492:Low Density Lipoprotein Receptor-Related Protein-5
keywords: Fat mass; LRP5; Lean mass; Quantitative ultrasound; SOST