title: Flavonol glycosides from the leaves of Elaeagnus pungens.
abstract: The leaves of Elaeagnus pungens were extracted and successively purified by a set chromatographic techniques. The structures of the obtained constituents were elucidated on the basis of spectroscopic methods including 1D/2D NMR and high resolution-MS. One new flavonoid glycosides, kaempferol 3-O-β-d-glucopyranosyl-(1 → 3)-α-l-rhamnopyranosyl-(1 → 6)-[α-l-rhamnopyranosyl(1 → 2)]-β-d-galactopyranoside-7-O-β-d-glucopyranoside (1), along with five known compounds (2-6) were isolated and identified. The MTT assay in vitro showed that the isolated flavonol glycosides have no proliferating activity, suggesting no aggravating impact on the increased airway smooth muscle cells.
authors.affiliation: a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
authors.forename: Ying-Ying
authors.initials: YY
authors.lastname: Shang
authors.affiliation: a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Qin
authors.affiliation: a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
authors.forename: Meng-Shun
authors.initials: MS
authors.lastname: Li
authors.affiliation: a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
authors.forename: Hai-Yan
authors.initials: HY
authors.lastname: Xu
authors.affiliation: a School of Pharmaceutical Sciences , South-Central University for Nationalities , Wuhan , China.
authors.forename: Yue-Bin
authors.initials: YB
authors.lastname: Ge
mesh_terms: D000818:Animals; D031263:Elaeagnaceae / Q000737:chemistry*; D044948:Flavonols / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D006027:Glycosides / Q000737:chemistry / Q000302:isolation & purification* / Q000494:pharmacology; D008297:Male; D018515:Plant Leaves / Q000737:chemistry; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D044948:Flavonols; D006027:Glycosides
keywords: Elaeagnus pungens; flavonoid glycosides; proliferating activity; spectroscopic analysis