title: Disequilibrium in the CD8+CD28+/CD8+CD28-T Lymphocyte Balance Is Related to Prognosis in Rats with Trinitrobenzenesulfonic Acid-Induced Colitis.
abstract: The CD8+CD28+/CD8+CD28-T lymphocyte balance is vital for human ulcerative colitis (UC) but has not been defined in experimental colitis. This investigation will try to identify the changes that occur in the CD8+CD28+/CD8+CD28-T lymphocyte balance during the progression of trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats.
  
  
  
  The frequencies of blood CD8+CD28+and CD8+CD28-T lymphocytes were detected in the rats belonging to the normal, model, and treated groups on five days using flow cytometry. The treated rats were administered with mesalazine and were euthanized after a 14-day treatment, as were the normal and model rats. The sensitivity and specificity of the CD8+CD28+/CD8+CD28-T lymphocyte balance in diagnosing early colitis were analyzed by receiver operating characteristics (ROC) curves. The frequencies of CD8+CD28+and CD8+CD28-T lymphocytes in the colon tissue were tested via immunofluorescence. ELISA was used to measure the levels of the cytokines. Immunohistochemistry and Western blotting were used to detect the colonic expression of JAK3, STAT6, NFATc2, and GATA3.
  
  
  
  We found that the ratio of CD8+CD28+/CD8+CD28-T lymphocytes decreased, as did the level of interleukin-7, but not IL-12p40, IL-13, or IL-15, in the blood; however, the ratio increased along with JAK3, STAT6, NFATc2, and GATA3 in the colon of the rats with colitis. The changes were effectively reversed through the administration of mesalazine for 13 days. Surprisingly, the balance in the blood could sensitively distinguish rats with early colitis from normal rats.
  
  
  
  These data show that increase in CD8+CD28+T cells in blood and decrease in CD8+CD28-T cells in colon are associated with experimental colitis.
authors.affiliation: Department of Rheumatology, TCM-Integrated Hospital, Southern Medical University, No. 13, Shiliugang Road, Haizhu District, Guangzhou, 510315, Guangdong, People's Republic of China. shixuedai@hotmail.com.|Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China. shixuedai@hotmail.com.|Department of Gastroenterology, Guangdong General Hospital and Guangdong Academy of Medical Sciences, South China University of Technology, Guangzhou, 510080, People's Republic of China. shixuedai@hotmail.com.
authors.forename: Shixue
authors.initials: S
authors.lastname: Dai
authors.affiliation: Department of Gastroenterology, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Hongxiang
authors.initials: H
authors.lastname: Gu
authors.affiliation: Undergraduate of Grade 2013, The First Clinical College, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Qianyi
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Department of Anatomy and Cell Biology, Brody School of Medicine, East Carolina University, Greenville, NC, 27834, USA.
authors.forename: Tiaosi
authors.initials: T
authors.lastname: Xing
authors.affiliation: Undergraduate of Grade 2013, School of Public Health, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Minhua
authors.initials: M
authors.lastname: Chen
authors.affiliation: Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Zhong
authors.affiliation: Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Gastroenterology, Huai'an First People's Hospital, Nanjing Medical University, Huai'an, 223001, Jiangsu, People's Republic of China.
authors.forename: Yanling
authors.initials: Y
authors.lastname: Feng
authors.affiliation: Department of Spleen and Stomach Diseases, Tai'an Hospital of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Tai'an, 271000, Shandong, People's Republic of China.
authors.forename: Hongbo
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Thoracic Surgery, The Fifth Affiliated Hospital, Southern Medical University, Guangzhou, 510900, Guangdong, People's Republic of China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Hongjian
authors.initials: H
authors.lastname: Jian
authors.affiliation: Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Minhai
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical University, Dongguan, 523808, Guangdong, People's Republic of China.
authors.forename: Hongmei
authors.initials: H
authors.lastname: Mo
authors.affiliation: Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical University, Dongguan, 523808, Guangdong, People's Republic of China.
authors.forename: Huanjie
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Dongsheng
authors.initials: D
authors.lastname: Chen
authors.affiliation: Department of Emergency, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, Guangdong, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical University, Dongguan, 523808, Guangdong, People's Republic of China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zou
authors.affiliation: Department of Traditional Chinese Medicine, The Second Clinical Medical College, Guangdong Medical University, Dongguan, 523808, Guangdong, People's Republic of China.
authors.forename: Honggang
authors.initials: H
authors.lastname: Chi
authors.affiliation: Guangdong Key Laboratory for Research and Development of Natural Drug, Research Institute of Traditional Chinese Medicine, Guangdong Medical University, Zhanjiang, 524023, Guangdong, People's Republic of China.
authors.forename: Yuzhen
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000008:administration & dosage; D015703:Antigens, CD / Q000032:analysis / Q000378:metabolism; D003092:Colitis / Q000175:diagnosis / Q000209:etiology / Q000276:immunology; D004195:Disease Models, Animal; D018450:Disease Progression; D042241:Early Diagnosis; D007378:Interleukins / Q000032:analysis / Q000378:metabolism; D019804:Mesalamine / Q000008:administration & dosage*; D011379:Prognosis; D012372:ROC Curve; D051381:Rats; D012680:Sensitivity and Specificity; D014302:Trinitrobenzenesulfonic Acid / Q000494:pharmacology
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D015703:Antigens, CD; D007378:Interleukins; D019804:Mesalamine; D014302:Trinitrobenzenesulfonic Acid
keywords: CD8+CD28+/CD8+CD28− T lymphocytes; Immunological balance; Inflammatory bowel disease; Ulcerative colitis