title: Screening of potential biomarkers for prenatal diagnosis of trisomy 21.
abstract: We aimed to identify key genes located on chromosome 21 as potential biomarkers for prenatal diagnosis of trisomy 21 (Ts21). The microarray data of GSE48051, including 10 cultivated amniocyte samples with Ts21 and 9 controls with normal euploid constitution, was obtained from Gene Expression Omnibus database. The differentially expressed genes (DEGs) in cultivated amniocyte samples with Ts21 compared to normal controls were screened using limma package. Then, we performed GO enrichment analysis using DAVID and chromosomal location of DEGs based on the information of the University of California Santa Cruz (UCSC) Genome Browser Database. Finally, protein-protein interaction (PPI) network analysis was performed using STRING. Total 155 DEGs in cultivated amniocyte samples with Ts21 were identified, including 89 up- and 66 down-regulated DEGs. The over-represented GO terms of DEGs were mainly related with apoptosis, programmed cell death and cell death. In total, 13 DEGs were located on chromosome 21, thereinto, only 6 DEGs were included into the PPI network, including superoxide dismutase 1 (SOD1), phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), downstream neighbour of SON (DONSON), ATP synthase, H + transporting, mitochondrial F1 complex, O subunit (ATP5O), chromatin assembly factor 1, subunit B (p60) (CHAF1B) and proteasome (prosome, macropain) assembly chaperone 1 (PSMG1). Our results suggest that SOD1, GART, DONSON, ATP5O, CHAF1B and PSMG1 may play important roles in the pathogenesis of Down syndrome and may serve as potential biomarkers for prenatal diagnosis of Ts21.
authors.affiliation: a Department of Pediatric Emergency , First Hospital of Jilin University , Changchun , Jilin , China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ma
authors.affiliation: a Department of Pediatric Emergency , First Hospital of Jilin University , Changchun , Jilin , China.
authors.forename: Feng
authors.initials: F
authors.lastname: Li
authors.affiliation: b Department of Clinical Laboratory , Hospital of Stomatology, Jilin University , Changchun , Jilin , China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Yu
authors.affiliation: a Department of Pediatric Emergency , First Hospital of Jilin University , Changchun , Jilin , China.
authors.forename: Haibo
authors.initials: H
authors.lastname: Li
mesh_terms: D016022:Case-Control Studies; D002891:Chromosomes, Human, Pair 21 / Q000235:genetics*; D004314:Down Syndrome / Q000175:diagnosis / Q000235:genetics*; D015536:Down-Regulation; D005260:Female; D015870:Gene Expression / Q000235:genetics*; D020869:Gene Expression Profiling / Q000379:methods*; D005819:Genetic Markers; D006801:Humans; D011247:Pregnancy; D011296:Prenatal Diagnosis / Q000379:methods*; D015854:Up-Regulation
chemical_list: D005819:Genetic Markers
keywords: Down syndrome; chromosomal location; differentially expressed genes; protein-protein interaction network