title: Transcriptome Analysis of the Intracellular Facultative Pathogen Piscirickettsia salmonis: Expression of Putative Groups of Genes Associated with Virulence and Iron Metabolism.
abstract: The intracellular facultative bacteria Piscirickettsia salmonis is one of the most important pathogens of the Chilean aquaculture. However, there is a lack of information regarding the whole genomic transcriptional response according to different extracellular environments. We used next generation sequencing (NGS) of RNA (RNA-seq) to study the whole transcriptome of an isolate of P. salmonis (FAVET-INBIOGEN) using a cell line culture and a modified cell-free liquid medium, with or without iron supplementation. This was done in order to obtain information about the factors there are involved in virulence and iron acquisition. First, the isolate was grown in the Sf21 cell line; then, the bacteria were cultured into a cell-free liquid medium supplemented or not with iron. We identified in the transcriptome, genes associated with type IV secretion systems, genes related to flagellar structure assembly, several proteases and sigma factors, and genes related to the development of drug resistance. Additionally, we identified for the first time several iron-metabolism associated genes including at least two iron uptake pathways (ferrous iron and ferric iron uptake) that are actually expressed in the different conditions analyzed. We further describe putative genes that are related with the use and storage of iron in the bacteria, which have not been previously described. Several sets of genes related to virulence were expressed in both the cell line and cell-free culture media (for example those related to flagellar structure; such as basal body, MS-ring, C-ring, proximal and distal rod, and filament), which may play roles in other basic processes rather than been restricted to virulence.
authors.affiliation: FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Machuca
authors.affiliation: FAVET-INBIOGEN, Faculty of Veterinary Sciences, University of Chile, Santiago, Chile.
authors.forename: Victor
authors.initials: V
authors.lastname: Martinez
mesh_terms: D001692:Biological Transport / Q000235:genetics; D005407:Flagella / Q000378:metabolism; D020869:Gene Expression Profiling; D042541:Intracellular Space / Q000382:microbiology*; D007501:Iron / Q000378:metabolism*; D018384:Oxidative Stress / Q000235:genetics; D053522:Piscirickettsia / Q000235:genetics* / Q000378:metabolism / Q000472:pathogenicity*; D014774:Virulence / Q000235:genetics
chemical_list: D007501:Iron