title: Activation and Stabilization of Olive Recombinant 13-Hydroperoxide Lyase Using Selected Additives.
abstract: The stabilization of olive recombinant hydroperoxide lyases (rHPLs) was investigated using selected chemical additives. Two rHPLs were studied: HPL full-length and HPL with its chloroplast transit peptide deleted (matured HPL). Both olive rHPLs are relatively stable at 4 °C, and enzyme activity can be preserved (about 100% of the rHPL activities are maintained) during 5 weeks of storage at -20 or at -80 °C in the presence of glycerol (10%, v/v). Among the additives used in this study, glycine (2.5% w/v), NaCl (0.5 M), and Na2SO4(0.25 M) provided the highest activation of HPL full-length activity, while the best matured HPL activity was obtained with Na2SO4(0.25 M) and NaCl (1 M). Although the inactivation rate constants (k) showed that these additives inactivate both rHPLs, their use is still relevant as they strongly increase HPL activity. Results of C6-aldehyde production assays also showed that glycine, NaCl, and Na2SO4are appropriate additives and that NaCl appears to be the best additive, at least for hexanal production.
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire Végétales, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Sabrina
authors.initials: S
authors.lastname: Jacopini
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire Végétales, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Vincenti
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire Végétales, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Magali
authors.initials: M
authors.lastname: Mariani
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire Végétales, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Brunini-Bronzini de Caraffa
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire Végétales, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Claude
authors.initials: C
authors.lastname: Gambotti
authors.affiliation: Laboratoire de Chimie des Produits Naturels, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Jean-Marie
authors.initials: JM
authors.lastname: Desjobert
authors.affiliation: Laboratoire de Chimie des Produits Naturels, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Muselli
authors.affiliation: Laboratoire de Chimie des Produits Naturels, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Costa
authors.affiliation: Equipe Chimie et Biomasse, Université de Corse, CNRS UMR6134 SPE, Route des Sanguinaires, 20000, Ajaccio, France.
authors.forename: Félix
authors.initials: F
authors.lastname: Tomi
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire Végétales, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France.
authors.forename: Liliane
authors.initials: L
authors.lastname: Berti
authors.affiliation: Laboratoire de Biochimie et Biologie Moléculaire Végétales, Université de Corse, CNRS UMR6134 SPE, Campus Grimaldi, BP52, 20250, Corte, France. maury@univ-corse.fr.
authors.forename: Jacques
authors.initials: J
authors.lastname: Maury
mesh_terms: D000446:Aldehyde-Lyases / Q000737:chemistry* / Q000235:genetics; D003577:Cytochrome P-450 Enzyme System / Q000737:chemistry* / Q000235:genetics; D004789:Enzyme Activation; D004795:Enzyme Stability; D031658:Olea / Q000201:enzymology* / Q000235:genetics; D010940:Plant Proteins / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D003577:Cytochrome P-450 Enzyme System; D000446:Aldehyde-Lyases; C054540:hydroperoxide lyase
keywords: 3Z-hexenal; Additives; Green note; Hexanal; Hydroperoxide lyase; Stabilization