title: A facile label-free G-quadruplex based fluorescent aptasensor method for rapid detection of ATP.
abstract: The present work demonstrates a simple, rapid and label-free ATP detection method using a fluorescent aptasensor that is based on G-quadruplex formation. In the absence of ATP, the Thioflavin T (ThT) dye binds to the G-rich ATP aptamer and forms an ATP aptamer/ThT G-quadruplex complex, which results in high fluorescence intensity. Upon addition of ATP, the ATP aptamer/ThT complex will be replaced by the formation of an ATP aptamer/ATP complex. During this process, separation of the ThT dye from the ATP aptamer/ThT complex decreases the fluorescence intensity of the reaction mixture dramatically. This fluorescence aptasensor is highly sensitive and rapid, with a detection limit of 18nM and a total reaction time of only 10min. Furthermore, this method is cost-effective and simple, removing the requirement for labeling the detection reagents with a fluorophore-quencher pair.
authors.affiliation: State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410013, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410013, China. Electronic address: macb2012@csu.edu.cn.
authors.forename: Changbei
authors.initials: C
authors.lastname: Ma
authors.affiliation: State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410013, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Ning
authors.affiliation: State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410013, China.
authors.forename: Hanchun
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410013, China.
authors.forename: Hailun
authors.initials: H
authors.lastname: He
authors.affiliation: State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410081, China.
authors.forename: Kemin
authors.initials: K
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Medical Genetics, School of Life Sciences, Central South University, Changsha 410013, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis* / Q000097:blood; D052157:Aptamers, Nucleotide / Q000737:chemistry*; D015374:Biosensing Techniques / Q000379:methods*; D005240:Feasibility Studies; D005456:Fluorescent Dyes / Q000737:chemistry*; D054856:G-Quadruplexes; D006801:Humans; D013050:Spectrometry, Fluorescence; D013194:Staining and Labeling
chemical_list: D052157:Aptamers, Nucleotide; D005456:Fluorescent Dyes; D000255:Adenosine Triphosphate
keywords: ATP; Aptasensor; G-quadruplex formation; Label-free; Thioflavin T