title: Disruption of non-anchored cell wall protein NCW-1 promotes cellulase production by increasing cellobiose uptake in Neurospora crassa.
abstract: OBJECTIVE
  To elucidate the mechanism of cellulase signal transduction in filamentous fungi including the components of the cellulase induction pathway.
  
  
  RESULTS
  Neurospora crassa ncw-1 encodes a non-anchored cell wall protein. The absence of ncw-1 increased cellulase gene expression and this is not due to relieving carbon catabolite repression mediated by the cre-1 pathway. A mutant lacking genes encoding both three major β-glucosidase enzymes and NCW-1 (Δ3βGΔncw-1) was constructed. Transcriptome analysis of the quadruple mutant demonstrated enhanced expression of cellodextrin transporters after ncw-1 deletion, indicating that ncw-1 affects cellulase expression and production by inhibiting the uptake of the cellodextrin.
  
  
  CONCLUSIONS
  NCW-1 is a novel component that plays a critical role in the cellulase induction signaling pathway.
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Liangcai
authors.initials: L
authors.lastname: Lin
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Jingen
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China.
authors.forename: Wenliang
authors.initials: W
authors.lastname: Sun
authors.affiliation: Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China. tian_cg@tib.cas.cn.
authors.forename: Chaoguang
authors.initials: C
authors.lastname: Tian
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D002475:Cellobiose / Q000378:metabolism*; D002480:Cellulase / Q000235:genetics / Q000378:metabolism*; D002482:Cellulose / Q000031:analogs & derivatives / Q000378:metabolism; D003912:Dextrins / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D015966:Gene Expression Regulation, Fungal; D026901:Membrane Transport Proteins / Q000235:genetics / Q000378:metabolism*; D009492:Neurospora crassa / Q000201:enzymology* / Q000235:genetics; D015398:Signal Transduction; D001617:beta-Glucosidase / Q000378:metabolism
chemical_list: D003912:Dextrins; D005656:Fungal Proteins; D026901:Membrane Transport Proteins; D002475:Cellobiose; D002482:Cellulose; C068994:cellodextrin; D001617:beta-Glucosidase; D002480:Cellulase
keywords: Cell wall protein; Cellobiose uptake; Cellodextrin transporter; Cellulase induction; Neurospora crassa