title: Brahma-related gene 1 induces apoptosis in a p53-dependent manner in human rheumatoid fibroblast-like synoviocyte MH7A.
abstract: Blocked apoptosis and aggressive inflammatory responses occur in fibroblast-like synoviocyte (FLS) of rheumatoid arthritis (RA) patients. Although Brahma-related gene 1 (BRG1) is considered as a tumor suppressor, few research covers its role in RA. This study aims to reveal effects and potential mechanisms of BRG1 in human FLS cell line MH7A.BRG1 expression in MH7A cells was altered by transfection of overexpression vectors or short hairpin RNAs (shRNAs). Cell viability and apoptosis were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry after transfection. Factors involved in inflammation and apoptosis were quantified by qPCR and Western blot. The interaction between BRG1 and p53 was assessed by immunoprecipitation (IP).Results showed that BRG1 overexpression significantly suppressed MH7A cell viability and induced apoptosis (P < 0.01), and its knockdown had opposite effects. BRG1 reduced mRNA levels of matrix metallopeptidase 3, TIMP metallopeptidase inhibitor 2, cyclooxygenase 2, and interleukin 6, implying its suppressive effects on inflammation. BRG1 interacted with and promoted p53 (P < 0.05). B-cell chronic lymphocytic leukemia/lymphoma 2 was suppressed (P < 0.05), while cytochrome c, caspase 3 (CASP3) and CASP9 were activated (P < 0.01) by BRG1. However, the regulation on these factors was abrogated by p53 knockdown (P < 0.01).These findings suggest that BRG1 may induce apoptosis and suppress inflammation in MH7A cells. Potential functional mechanisms involve the regulation of apoptotic factors by BRG1, which may depend on the recruitment and promotion of p53. This study provides the essential proof for applying BRG1 to the molecular therapy of RA.
authors.affiliation: Luoyang Orthopedic-traumatological Hospital of Henan Province, Orthopedic Hospital of Henan Province, Luoyang, China.
authors.forename: Hongli
authors.initials: H
authors.lastname: Hou
authors.forename: Weipeng
authors.initials: W
authors.lastname: Xing
authors.forename: Wuyin
authors.initials: W
authors.lastname: Li
mesh_terms: D017209:Apoptosis / Q000502:physiology*; D001172:Arthritis, Rheumatoid / Q000503:physiopathology*; D015153:Blotting, Western; D002460:Cell Line; D002470:Cell Survival / Q000502:physiology; D004265:DNA Helicases / Q000502:physiology*; D005434:Flow Cytometry; D006801:Humans; D007249:Inflammation / Q000503:physiopathology; D009687:Nuclear Proteins / Q000502:physiology*; D034741:RNA, Small Interfering / Q000378:metabolism; D000070918:Synoviocytes / Q000502:physiology*; D014157:Transcription Factors / Q000502:physiology*; D014162:Transfection; D016159:Tumor Suppressor Protein p53 / Q000502:physiology
chemical_list: D009687:Nuclear Proteins; D034741:RNA, Small Interfering; D014157:Transcription Factors; D016159:Tumor Suppressor Protein p53; C084108:SMARCA4 protein, human; D004265:DNA Helicases