title: Case-controlled study on indirect hyperbilirubinemia in exclusively breast fed neonates and mutations of the bilirubin Uridine Diphosphate-Glucuronyl transferase gene 1A1.
abstract: OBJECTIVE
  This study aims to investigate the association of neonatal indirect hyperbilirubinemia in exclusively breast-fed infants with UGT1A1 (Uridine Diphosphate-Glucuronyl transferase 1A1) polymorphism.
  
  
  METHODS
  50 neonates were classified into 2 groups: 1) 30 full term neonates with indirect hyperbilirubinemia (gestational age (GA) 39.5 ± 1.2 weeks); 2) 20 apparently healthy full-term neonates. Group 1 was further subdivided based on percentage of body weight lost: (A) less than 10%; (B) 10% or more.
  
  
  RESULTS
  There was a statistically significant decrease in weight at sample collection and significant increase in indirect bilirubin level in patients group compared to control group, there was statistically significant difference as regard to genotype frequency [G/G, G/A, A/A], and allele frequency (A,G) between patients and control group. There was statistically significant increase in indirect bilirubin level in G/A - A/A genotypes. By comparing subgroup (A) and subgroup (B), there was statistically significant increase in total bilirubin level in subgroup (B). There was statistically high significant difference regarding genotype frequency (G/G, G/A, A/A) and allele frequency (G, A) between subgroup A and B. Multiple stepwise regression analysis was done using hyperbilirubinemia as a dependent factor and body weight loss, genotype (G/A) and allele (A) as independent factors. Body weight loss, genotype (G/A) and allele (A) was found to be significant independent predictors for hyperbilirubinemia.
  
  
  CONCLUSIONS
  The results of the present study revealed that UGT1A1 polymorphism can be used as a novel predictor for neonatal hyperbilirubinemia in breast fed full term neonates.
authors.affiliation: Clinical & Chemical Pathology Department, Faculty of Medicine, Benha University, Egypt.
authors.forename: Amal E
authors.initials: AE
authors.lastname: Mohammed
authors.affiliation: Clinical & Chemical Pathology Department, Faculty of Medicine, Benha University, Egypt.
authors.forename: Eman G
authors.initials: EG
authors.lastname: Behiry
authors.affiliation: Pediatric Department, Faculty of Medicine, Benha University, Egypt.
authors.forename: Akram E
authors.initials: AE
authors.lastname: El-Sadek
authors.affiliation: Pediatric Department, Faculty of Medicine, Benha University, Egypt.
authors.forename: Waleed E
authors.initials: WE
authors.lastname: Abdulghany
authors.affiliation: Clinical & Chemical Pathology Department, Faculty of Medicine, Benha University, Egypt.
authors.forename: Dalia M
authors.initials: DM
authors.lastname: Mahmoud
authors.affiliation: Pediatric Department, Faculty of Medicine, Benha University, Egypt.
authors.forename: Abdelfattah A
authors.initials: AA
authors.lastname: Elkholy
keywords: Neonatal hyperbilirubinemia; UGT1A1 polymorphism