title: Prohibitin 2 Is an Inner Mitochondrial Membrane Mitophagy Receptor.
abstract: The removal of unwanted or damaged mitochondria by autophagy, a process called mitophagy, is essential for key events in development, cellular homeostasis, tumor suppression, and prevention of neurodegeneration and aging. However, the precise mechanisms of mitophagy remain uncertain. Here, we identify the inner mitochondrial membrane protein, prohibitin 2 (PHB2), as a crucial mitophagy receptor involved in targeting mitochondria for autophagic degradation. PHB2 binds the autophagosomal membrane-associated protein LC3 through an LC3-interaction region (LIR) domain upon mitochondrial depolarization and proteasome-dependent outer membrane rupture. PHB2 is required for Parkin-induced mitophagy in mammalian cells and for the clearance of paternal mitochondria after embryonic fertilization in C. elegans. Our findings pinpoint a conserved mechanism of eukaryotic mitophagy and demonstrate a function of prohibitin 2 that may underlie its roles in physiology, aging, and disease.
authors.affiliation: Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA.
authors.forename: Yongjie
authors.initials: Y
authors.lastname: Wei
authors.affiliation: Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA.
authors.forename: Wei-Chung
authors.initials: WC
authors.lastname: Chiang
authors.affiliation: Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA.
authors.forename: Rhea
authors.initials: R
authors.lastname: Sumpter
authors.affiliation: Children's Medical Center Research Institute, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA.
authors.forename: Prashant
authors.initials: P
authors.lastname: Mishra
authors.affiliation: Center for Autophagy Research, Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA; Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA; Department of Microbiology, University of Texas Southwestern Medical Center, Dallas, TX 75230, USA. Electronic address: beth.levine@utsouthwestern.edu.
authors.forename: Beth
authors.initials: B
authors.lastname: Levine
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000071182:Autophagosomes / Q000378:metabolism; D017173:Caenorhabditis elegans / Q000378:metabolism*; D029742:Caenorhabditis elegans Proteins / Q000378:metabolism; D004625:Embryo, Nonmammalian / Q000378:metabolism; D008565:Membrane Proteins / Q000378:metabolism; D051336:Mitochondrial Membranes / Q000378:metabolism*; D000091402:Prohibitins; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D029742:Caenorhabditis elegans Proteins; C000618573:LC3 protein, C elegans; D008565:Membrane Proteins; D000091402:Prohibitins; D012097:Repressor Proteins; C000717338:prohibitin 2, C elegans
keywords: autophagy; mitophagy; prohibitin 2