title: The General Expression Analysis of WTX Gene in Normal and Cancer Tissues.
abstract: WTX (Wilms' tumor suppressor X chromosome) is a novel putative tumor suppressor gene in Wilms' tumor of kidney, its expression and function in other human cancers had not been explored. This study detected the expression of WTX in 459 cases of 15 organs of cancers and adjacent normal tissues by using immunohistochemical staining (IHC), and validated them by in situ hybridization (ISH) and quantitative real-time reverse transcription PCR (qRT-PCR). IHC and ISH data showed that WTX protein was generally expressed in normal tissues, but reduced expression in corresponding cancers. This study demonstrated that WTX downregulation is a common phenomenon in human cancers, WTX might be a general tumor-suppressor gene and biological marker of multiple cancer tissues. Apart from kidney, stomach is another target tissue of WTX gene. The germline and somatic mutations of WTX were screened in 12 gastric cancer patients and identified in one cases (8.3%). Mutation in the WTX gene might be one of the reasons of WTX loss in gastric cancer patients.
authors.affiliation: Department of Pathology, Nanfang Hospital, Guangzhou, 510515, People's Republic of China.|Department of Pathology, College of Basic Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China.
authors.forename: Yao-Yao
authors.initials: YY
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Nanfang Hospital, Guangzhou, 510515, People's Republic of China.|Department of Pathology, College of Basic Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China.
authors.forename: Qi-Ming
authors.initials: QM
authors.lastname: Wang
authors.affiliation: Department of Pathology, Nanfang Hospital, Guangzhou, 510515, People's Republic of China.|Department of Pathology, College of Basic Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China.
authors.forename: Hui-Lin
authors.initials: HL
authors.lastname: Niu
authors.affiliation: Department of Pathology, Nanfang Hospital, Guangzhou, 510515, People's Republic of China.|Department of Pathology, College of Basic Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China.
authors.forename: Xia
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Pathology, Nanfang Hospital, Guangzhou, 510515, People's Republic of China. zqllc8@126.com.|Department of Pathology, College of Basic Medicine, Southern Medical University, Guangzhou, 510515, People's Republic of China. zqllc8@126.com.
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Zhang
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics*; D014408:Biomarkers, Tumor / Q000235:genetics; D015536:Down-Regulation / Q000235:genetics; D015870:Gene Expression / Q000235:genetics*; D006801:Humans; D009154:Mutation / Q000235:genetics; D009369:Neoplasms / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000235:genetics*
chemical_list: C517118:AMER1 protein, human; D048868:Adaptor Proteins, Signal Transducing; D014408:Biomarkers, Tumor; D025521:Tumor Suppressor Proteins
keywords: Immunohistochemical staining; In situ hybridization; Tumor suppressor; Wilms’ tumor suppressor X chromosome (WTX)