title: Prevascularization of Decellularized Porcine Myocardial Slice for Cardiac Tissue Engineering.
abstract: Prevacularization strategies have been implemented in tissue engineering to generate microvasculature networks within a scaffold prior to implantation. Prevascularizing scaffolds will shorten the time of functional vascular perfusion with host upon implantation. In this study, we explored key variables affecting the interaction between decellularized porcine myocardium slices (dPMSs) and reseeded stem cells toward the fabrication of prevascularized cardiac tissue. Our results demonstrated that dPMS supports attachment of human mesenchymal stem cells (hMSCs) and rat adipose derived stem cells (rASCs) with high viability. We found that cell seeding efficiency and proliferation are dPMS thickness dependent. Compared to lateral cell seeding, bilateral cell seeding strategy significantly enhanced seeding efficiency, infiltration, and growth in 600 μm dPMS. dPMS induced endothelial differentiation and maturation of hMSCs and rASCs after 1 and 5 days culture, respectively. These results indicate the potential of dPMS as a powerful platform to develop prevascularized scaffolds and fabricate functional cardiac patches.
authors.affiliation: Department of Biomedical Engineering, The University of Akron ,Akron, Ohio 44325, United States.
authors.forename: Pawan
authors.identifier: http://orcid.org/0000-0001-7565-8948
authors.initials: P
authors.lastname: Kc
authors.affiliation: Department of Biomedical Engineering, The University of Akron ,Akron, Ohio 44325, United States.|Integrated Bioscience Program, The University of Akron , Akron, Ohio 44325, United States.
authors.forename: Mickey
authors.initials: M
authors.lastname: Shah
authors.affiliation: Department of Bioengineering, University of Texas at Arlington , Arlington, Texas 76019, United States.
authors.forename: Jun
authors.initials: J
authors.lastname: Liao
authors.affiliation: Department of Biomedical Engineering, The University of Akron ,Akron, Ohio 44325, United States.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006801:Humans; D059630:Mesenchymal Stem Cells; D009206:Myocardium; D051381:Rats; D013234:Stem Cells; D013552:Swine; D023822:Tissue Engineering; D054457:Tissue Scaffolds
keywords: cardiac tissue engineering; decellularized extracellular matrix; human mesenchymal stem cells; prevascularization; rat adipose derived stem cells; stem cell differentiation