title: Studying the Interaction of Magainin 2 and Cecropin A with E. coli Bacterial Cells Using Circular Dichroism.
abstract: The potential of antimicrobial peptides (AMPs) as an effective therapeutic alternative to classic and current antibiotics has encouraged studies to understand how they interact with the bacterial membrane. Here we describe how to detect, by circular dichroism (CD), the secondary structures of two antimicrobial peptides, magainin 2 and cecropin A, in the presence of E. coli bacterial cells.
authors.affiliation: Institute of Biostructure and Bioimaging CNR, via Mezzocannnone 16, 80134, Naples, Italy.
authors.forename: Concetta
authors.initials: C
authors.lastname: Avitabile
authors.affiliation: Institute of Biostructure and Bioimaging CNR, via Mezzocannnone 16, 80134, Naples, Italy.
authors.forename: Luca Domenico
authors.initials: LD
authors.lastname: D'Andrea
authors.affiliation: Institute of Biostructure and Bioimaging CNR, via Mezzocannnone 16, 80134, Naples, Italy. alessandra.romanelli@unina.it.|Department of Pharmacy, University of Naples "Federico II", via Mezzocannone 16, Naples, 80134, Italy. alessandra.romanelli@unina.it.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Romanelli
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D002942:Circular Dichroism / Q000379:methods; D004926:Escherichia coli / Q000187:drug effects / Q000378:metabolism; D054806:Magainins / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D011485:Protein Binding; D017433:Protein Structure, Secondary
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D054806:Magainins; C031161:cecropin A
keywords: Antimicrobial peptides; Circular dichroism; E. coli cells