title: A Novel Tool to Measure Extracellular Glutamate in the Zebrafish Nervous System In Vivo.
abstract: Glutamate is the major excitatory neurotransmitter in the brain. Its release and eventual recycling are key to rapid sustained neural activity. We have paired the gfap promoter region with the glutamate reporter molecule, iGluSnFR, to drive expression in glial cells throughout the nervous system. Tg(gfap:iGluSnFR) is expressed on the glial membrane of Müller glia cells in the retina, which rapidly respond to stimulation and the release of extracellular glutamate. As glial cells are associated with most, if not all, synapses, Tg(gfap:iGluSnFR) is a novel and exciting tool to measure neuronal activity and extracellular glutamate dynamics in many regions of the nervous system.
authors.affiliation: 1 Department of Infection, Immunity and Cardiovascular Disease, Medical School and the Bateson Centre, University of Sheffield , Sheffield, United Kingdom .|2 Department of Physiology, Development and Neuroscience, University of Cambridge , Cambridge, United Kingdom .
authors.forename: Ryan B
authors.initials: RB
authors.lastname: MacDonald
authors.affiliation: 3 Sussex Neuroscience, School of Life Sciences, University of Sussex , Brighton, United Kingdom .
authors.forename: Nachiket D
authors.initials: ND
authors.lastname: Kashikar
authors.affiliation: 3 Sussex Neuroscience, School of Life Sciences, University of Sussex , Brighton, United Kingdom .
authors.forename: Leon
authors.initials: L
authors.lastname: Lagnado
authors.affiliation: 2 Department of Physiology, Development and Neuroscience, University of Cambridge , Cambridge, United Kingdom .
authors.forename: William A
authors.initials: WA
authors.lastname: Harris
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified / Q000254:growth & development / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000378:metabolism*; D005786:Gene Expression Regulation; D018698:Glutamic Acid / Q000378:metabolism*; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D009420:Nervous System / Q000166:cytology / Q000378:metabolism*; D009457:Neuroglia / Q000166:cytology / Q000378:metabolism; D011401:Promoter Regions, Genetic; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D012160:Retina / Q000166:cytology / Q000378:metabolism; D015027:Zebrafish / Q000254:growth & development / Q000378:metabolism*
chemical_list: D029968:Escherichia coli Proteins; D011993:Recombinant Fusion Proteins; C579254:iGluSnFR protein; D049452:Green Fluorescent Proteins; D018698:Glutamic Acid
keywords: glia; glutamate; nervous system; retina