title: Evaluation of antibacterial, antioxidant and nootropic activities of Tiliacora racemosa Colebr. leaves: In vitro and in vivo approach.
abstract: The antibacterial and antioxidant potential of Tiliacora racemosa leaf extracts in various solvents (methanolic, hexane, chloroform and ethyl acetate) was determined. Additionally, the presence of bisbenzylisoquinoline alkaloids in the plant prompted us to evaluate the nootropic activity of the methanolic extract in mice. Further, we seek to verify the nootropic effect by examining the anticholinesterase inhibition potential of the methanolic extract. The leaf extracts in various solvents were evaluated for their antibacterial and antioxidant activity by agar diffusion technique and α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method, respectively. The ex vivo acetylcholine esterase inhibitory activity of the methanolic extract was carried out by Ellman's method in male Wistar rats. The nootropic capacity of the methanolic extract was examined in Swiss albino mice by utilizing the diazepam induced acute amnesic model. The chloroform/n-hexane and ethyl acetate fraction showed promising antioxidant and antibacterial (Gram positive and Gram negative bacteria) property, respectively. The methanolic extract was able to diminish the amnesic effect induced by diazepam (1mg/kg i.p.) in mice. The extract also showed significant acetyl cholinesterase inhibition in rats. The findings prove that the memory enhancing capability is due to increased acetyl choline level at the nerve endings. The strong antioxidant nature and potential nootropic activity shown by the extract suggests its future usage in the treatment of neurodegenerative disorders such as dementia and Alzheimer.
authors.affiliation: Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Osmania University, Hyderabad, Telangana State, India.
authors.forename: Vivek Kumar
authors.initials: VK
authors.lastname: T
authors.affiliation: Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Osmania University, Hyderabad, Telangana State, India.
authors.forename: Vishalakshi
authors.initials: V
authors.lastname: M
authors.affiliation: Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Osmania University, Hyderabad, Telangana State, India.
authors.forename: Gangaraju
authors.initials: G
authors.lastname: M
authors.affiliation: Department of Microbiology, St. Xavier's College, Kolkata, West Bengal, India.
authors.forename: Parijat
authors.initials: P
authors.lastname: Das
authors.affiliation: Department of Microbiology, St. Xavier's College, Kolkata, West Bengal, India.
authors.forename: Pratiti
authors.initials: P
authors.lastname: Roy
authors.affiliation: Department of Microbiology, St. Xavier's College, Kolkata, West Bengal, India.
authors.forename: Anindita
authors.initials: A
authors.lastname: Banerjee
authors.affiliation: Department of Pharmaceutical Chemistry, Gokaraju Rangaraju College of Pharmacy, Osmania University, Telangana State, Hyderabad 500090, India. Electronic address: sayanduttagupta1@rediffmail.com.
authors.forename: Sayan
authors.initials: S
authors.lastname: Dutta Gupta
mesh_terms: D000470:Alkaloids / Q000737:chemistry / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002800:Cholinesterase Inhibitors / Q000737:chemistry / Q000494:pharmacology; D016166:Free Radical Scavengers / Q000737:chemistry / Q000494:pharmacology; D006586:Hexanes / Q000737:chemistry; D008297:Male; D029583:Menispermaceae / Q000737:chemistry*; D051379:Mice; D018697:Nootropic Agents / Q000494:pharmacology*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018515:Plant Leaves / Q000737:chemistry*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000470:Alkaloids; D000900:Anti-Bacterial Agents; D000975:Antioxidants; D002800:Cholinesterase Inhibitors; D016166:Free Radical Scavengers; D006586:Hexanes; D018697:Nootropic Agents; D010936:Plant Extracts; C026385:n-hexane
keywords: Antibacterial; Antioxidant; Bisbenzylisoquinoline alkaloids; Nootropic; Tiliacora racemosa