title: Electrohydrodynamic-Induced SERS Immunoassay for Extensive Multiplexed Biomarker Sensing.
abstract: Cancer diagnosis and patient monitoring require sensitive and simultaneous measurement of multiple cancer biomarkers considering that single biomarker analysis present inadequate information on the underlying biological transformations. Thus, development of sensitive and selective assays for multiple biomarker detection might improve clinical diagnosis and expedite the treatment process. Herein, a microfluidic platform for the rapid, sensitive, and parallel detection of multiple cancer-specific protein biomarkers from complex biological samples is presented. This approach utilizes alternating current electrohydrodynamic-induced surface shear forces that provide exquisite control over fluid flow thereby enhancing target-sensor interactions and minimizing non-specific binding. Further, the use of surface-enhanced Raman scattering-based spectral encoding with individual barcodes for different targets enables specific and simultaneous detection of captured protein biomarkers. Using this approach, the specific and sensitive detection of clinically relevant biomarkers including human epidermal growth factor receptor 2 (HER2); Mucin 1, cell surface associated (MUC1); epidermal growth factor receptor; and Mucin 16, cell surface associated (MUC16) at concentrations as low as 10 fg mL-1in patient serum is demonstrated. Successful target detection from patient samples further demonstrates the potential of this current approach for the clinical diagnosis, which envisages a clinical translation for a rapid and sensitive appraisal of clinical samples in cancer diagnostics.
authors.affiliation: Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner College and Cooper Roads (Bldg 75), Brisbane, QLD, 4072, Australia.
authors.forename: Khondakar
authors.initials: K
authors.lastname: Kamil Reza
authors.affiliation: Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner College and Cooper Roads (Bldg 75), Brisbane, QLD, 4072, Australia.
authors.forename: Jing
authors.initials: J
authors.lastname: Wang
authors.affiliation: Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner College and Cooper Roads (Bldg 75), Brisbane, QLD, 4072, Australia.
authors.forename: Ramanathan
authors.initials: R
authors.lastname: Vaidyanathan
authors.affiliation: Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner College and Cooper Roads (Bldg 75), Brisbane, QLD, 4072, Australia.
authors.forename: Shuvashis
authors.initials: S
authors.lastname: Dey
authors.affiliation: Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner College and Cooper Roads (Bldg 75), Brisbane, QLD, 4072, Australia.
authors.forename: Yuling
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Centre for Personalised Nanomedicine, Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Corner College and Cooper Roads (Bldg 75), Brisbane, QLD, 4072, Australia.|School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
authors.forename: Matt
authors.initials: M
authors.lastname: Trau
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood*; D015374:Biosensing Techniques / Q000379:methods*; D004560:Electricity; D066246:ErbB Receptors / Q000378:metabolism; D006801:Humans; D057446:Hydrodynamics; D007118:Immunoassay / Q000379:methods*; D013059:Spectrum Analysis, Raman
chemical_list: D014408:Biomarkers, Tumor; D066246:ErbB Receptors
keywords: SERS; ac-EHD; cancer biomarkers; microfluidic devices; multiplexed detection