title: FGF11 induced by hypoxia interacts with HIF-1α and enhances its stability.
abstract: Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1α (HIF-1α) as an interacting protein of FGF11 using immunoprecipitation and mass spectrometry. FGF11 knockdown decreased HIF-1α protein, while FGF11 overexpression increased it, without affecting HIF-1α mRNA. Protein stability test and ubiquitination assay showed that FGF11 increased HIF-1α stability by acting upstream of proteasomal degradation. Altogether, these results suggest a cross-regulation between HIF-1α and FGF11, through which hypoxia-induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF-1α.
authors.affiliation: Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Korea.
authors.forename: Kyeong Won
authors.initials: KW
authors.lastname: Lee
authors.affiliation: Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Korea.
authors.forename: Hyung-Soon
authors.initials: HS
authors.lastname: Yim
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Jihye
authors.initials: J
authors.lastname: Shin
authors.affiliation: Center for Theragnosis, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul, Korea.
authors.forename: Cheolju
authors.initials: C
authors.lastname: Lee
authors.affiliation: Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Korea.|Department of Marine Biotechnology, Korea University of Science and Technology, Daejeon, Korea.
authors.forename: Jung-Hyun
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Marine Biotechnology Research Center, Korea Institute of Ocean Science and Technology, Ansan, Korea.
authors.forename: Jae-Yeon
authors.initials: JY
authors.lastname: Jeong
mesh_terms: D015687:Cell Hypoxia; D045744:Cell Line, Tumor; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D005786:Gene Expression Regulation; D055785:Gene Knockdown Techniques; D006801:Humans; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D011485:Protein Binding; D055550:Protein Stability; D059748:Proteolysis; D014158:Transcription, Genetic
chemical_list: C000609548:FGF11 protein, human; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D005346:Fibroblast Growth Factors
keywords: FGF11; HIF-1α; hypoxia; intracellular fibroblast growth factor