title: Piper betle Induced Cytoprotective Genes and Proteins via the Nrf2/ARE Pathway in Aging Mice.
abstract: The objective of this study was to elucidate the underlying antioxidant mechanism of aqueous extract of Piper betle (PB) in aging rats. The nuclear factor erythroid 2-related factor 2 (Nrf2)/ARE pathway involving phase II detoxifying and antioxidant enzymes plays an important role in the antioxidant system by reducing electrophiles and reactive oxygen species through induction of phase II enzymes and proteins.
  
  
  
  Genes and proteins of phase II detoxifying antioxidant enzymes were analyzed by QuantiGenePlex 2.0 Assay and Western blot analysis.
  
  
  
  PB significantly induced genes and proteins of phase II and antioxidant enzymes, NAD(P)H quinone oxidoreductase 1, and catalase in aging mice (p < 0.05). The expression of these enzymes were stimulated via translocation of Nrf2 into the nucleus, indicating the involvement of ARE, a cis-acting motif located in the promoter region of nearly all phase II genes.
  
  
  
  PB was testified for the first time to induce cytoprotective genes through the Nrf2/ARE signaling pathway, thus unraveling the antioxidant mechanism of PB during the aging process.
authors.affiliation: Department of Biochemistry, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
authors.forename: Nor Syahida
authors.initials: NS
authors.lastname: Aliahmat
authors.forename: Nur Fathiah
authors.initials: NF
authors.lastname: Abdul Sani
authors.forename: Wan Nuraini
authors.initials: WN
authors.lastname: Wan Hasan
authors.forename: Suzana
authors.initials: S
authors.lastname: Makpol
authors.forename: Wan Zurinah
authors.initials: WZ
authors.lastname: Wan Ngah
authors.forename: Yasmin Anum
authors.initials: YA
authors.lastname: Mohd Yusof
mesh_terms: D000375:Aging / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D000818:Animals; D061745:Antioxidant Response Elements / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D000975:Antioxidants / Q000494:pharmacology; D019610:Cytoprotection / Q000187:drug effects / Q000235:genetics; D008297:Male; D050217:Metabolic Detoxication, Phase II / Q000235:genetics; D051379:Mice; D008810:Mice, Inbred C57BL; D051267:NF-E2-Related Factor 2 / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects* / Q000235:genetics; D031721:Piper betle / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011506:Proteins / Q000187:drug effects / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D000975:Antioxidants; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse; D010936:Plant Extracts; D011506:Proteins