title: Fall Armyworm-Associated Gut Bacteria Modulate Plant Defense Responses.
abstract: Mechanical damage caused by insect feeding along with components present in insect saliva and oral secretions are known to induce jasmonic acid-mediated defense responses in plants. This study investigated the effects of bacteria from oral secretions of the fall armyworm Spodoptera frugiperda on herbivore-induced defenses in tomato and maize plants. Using culture-dependent methods, we identified seven different bacterial isolates belonging to the family Enterobacteriacea from the oral secretions of field-collected caterpillars. Two isolates, Pantoea ananatis and Enterobacteriaceae-1, downregulated the activity of the plant defensive proteins polyphenol oxidase and trypsin proteinase inhibitors (trypsin PI) but upregulated peroxidase (POX) activity in tomato. A Raoultella sp. and a Klebsiella sp. downregulated POX but upregulated trypsin PI in this plant species. Conversely, all of these bacterial isolates upregulated the expression of the herbivore-induced maize proteinase inhibitor (mpi) gene in maize. Plant treatment with P. ananatis and Enterobacteriaceae-1 enhanced caterpillar growth on tomato but diminished their growth on maize plants. Our results highlight the importance of herbivore-associated microbes and their ability to mediate insect plant interactions differently in host plants fed on by the same herbivore.
authors.affiliation: 1 Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, 16802, U.S.A.
authors.forename: Flor E
authors.initials: FE
authors.lastname: Acevedo
authors.affiliation: 1 Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, 16802, U.S.A.
authors.forename: Michelle
authors.initials: M
authors.lastname: Peiffer
authors.affiliation: 1 Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, 16802, U.S.A.
authors.forename: Ching-Wen
authors.initials: CW
authors.lastname: Tan
authors.affiliation: 2 Section of Research Resources, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, 17033, U.S.A.
authors.forename: Bruce A
authors.initials: BA
authors.lastname: Stanley
authors.affiliation: 2 Section of Research Resources, The Pennsylvania State University College of Medicine, 500 University Drive, Hershey, 17033, U.S.A.
authors.forename: Anne
authors.initials: A
authors.lastname: Stanley
authors.affiliation: 1 Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, 16802, U.S.A.|3 Department of Ecology, South China Agricultural University, Guangzhou, Guangdong 510640, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Wang
authors.affiliation: 1 Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, 16802, U.S.A.
authors.forename: Asher G
authors.initials: AG
authors.lastname: Jones
authors.affiliation: 1 Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, 16802, U.S.A.
authors.forename: Kelli
authors.initials: K
authors.lastname: Hoover
authors.affiliation: 4 Department of Plant Pathology, The Pennsylvania State University, 321 Buckhout Lab; and.
authors.forename: Cristina
authors.initials: C
authors.lastname: Rosa
authors.affiliation: 5 Department of Plant Science, The Pennsylvania State University, 216 Agricultural Sciences and Industries Building.
authors.forename: Dawn
authors.initials: D
authors.lastname: Luthe
authors.affiliation: 1 Department of Entomology, The Pennsylvania State University, 501 Agricultural Sciences and Industries Building, University Park, 16802, U.S.A.
authors.forename: Gary
authors.initials: G
authors.lastname: Felton
mesh_terms: D000818:Animals; D001419:Bacteria / Q000302:isolation & purification; D000069196:Gastrointestinal Microbiome; D060434:Herbivory; D019476:Insect Proteins / Q000378:metabolism; D007814:Larva / Q000254:growth & development; D018551:Solanum lycopersicum / Q000276:immunology* / Q000469:parasitology; D012463:Saliva / Q000201:enzymology; D012471:Salivary Proteins and Peptides / Q000378:metabolism; D018411:Spodoptera / Q000382:microbiology*; D015430:Weight Gain; D003313:Zea mays / Q000276:immunology* / Q000469:parasitology
chemical_list: D019476:Insect Proteins; D012471:Salivary Proteins and Peptides