title: Invertible micellar polymer nanoassemblies target bone tumor cells but not normal osteoblast cells.
abstract: OBJECTIVE
  To demonstrate the capability of the invertible micellar polymer nanoassemblies (IMAs) to deliver and release curcumin using the recently discovered mechanism of macromolecular inversion to treat bone tumor cells.
  
  
  METHODS
  The effect of IMA-mediated delivery of curcumin on osteosarcoma cell survival was investigated using MTS assays. To assess the effect of IMAs-delivered curcumin on osteosarcoma cell growth, fluorescence-activated cell sorting was performed. The uptake of micellar nanoassemblies was followed using confocal microscopy.
  
  
  CONCLUSIONS
  IMAs-delivered curcumin is effective in blocking osteosarcoma cell growth. It decreases cell viability in human osteosarcoma (MG63, KHOS, and LM7) cells while having no effect on normal human osteoblast cells. It indicates that curcumin-loaded IMAs provide a unique delivery system targeted to osteosarcoma cells.
authors.affiliation: Department of Coatings & Polymeric Materials, North Dakota State University, Fargo, ND 58105-6050, USA (currently Department of Science & Technology University of Twente, the Netherlands); Department of Coatings & Polymeric Materials, North Dakota State University, Fargo, ND 58105-6050, USA (currently Department of Science & Technology University of Twente, the Netherlands).
authors.forename: Olena
authors.initials: O
authors.lastname: Kudina
authors.affiliation: Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA; Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Shogren
authors.affiliation: Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA; Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Carl T
authors.initials: CT
authors.lastname: Gustafson
authors.affiliation: Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA; Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Yaszemski
authors.affiliation: Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA; Department of Orthopedics, Mayo Clinic, Rochester, MN 55905, USA.
authors.forename: Avudaiappan
authors.initials: A
authors.lastname: Maran
authors.affiliation: Department of Coatings & Polymeric Materials, North Dakota State University, Fargo, ND 58105-6050, USA; Department of Coatings & Polymeric Materials, North Dakota State University, Fargo, ND 58105-6050, USA.
authors.forename: Andriy
authors.initials: A
authors.lastname: Voronov
keywords: bone tumor treatment; curcumin delivery; invertible polymer micelles; micellar nanoassemblies