title: Biosynthesis of uniformly labeled13C- and14C-arachidonic acid in Mortierella alpina.
abstract: Arachidonic acid (ARA) is one of the most abundant polyunsaturated fatty acids (PUFAs) in the mammalian brain. Many enzymatically- and nonenzymatically-produced metabolic products have important and potent pharmacological properties. However, uniformly isotope labeled forms of ARA are not commercially available for studying the metabolic fates of ARA. This study describes a simple and efficient protocol for the biosynthesis of U-13C-ARA from U-13C-glucose, and U-14C-ARA from U-14C-glucose by Mortierella alpina. The protocols yield approximately 100nmol quantities of U-13C-ARA with an isotopic purity of 95% from a 500μl batch volume, and approximately 2μCi quantities of U-14C-ARA with an apparent specific activity in excess of 1200Ci/mol from a 250μl batch volume.
authors.affiliation: Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104, United States.
authors.forename: Jin V
authors.initials: JV
authors.lastname: Lee
authors.affiliation: Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104, United States.
authors.forename: Ran
authors.initials: R
authors.lastname: Furman
authors.affiliation: Department of Pharmacology, University of Pennsylvania, Philadelphia, PA 19104, United States; Departments of Biochemistry and Biophysics, and Medicine, University of Pennsylvania, Philadelphia, PA 19104, United States. Electronic address: axe@pharm.med.upenn.edu.
authors.forename: Paul H
authors.initials: PH
authors.lastname: Axelsen
mesh_terms: D016718:Arachidonic Acid / Q000096:biosynthesis* / Q000737:chemistry*; D002247:Carbon Isotopes / Q000737:chemistry*; D002250:Carbon Radioisotopes / Q000737:chemistry; D005947:Glucose / Q000378:metabolism; D007553:Isotope Labeling / Q000379:methods*; D020094:Mortierella / Q000378:metabolism*
chemical_list: D002247:Carbon Isotopes; D002250:Carbon Radioisotopes; D016718:Arachidonic Acid; D005947:Glucose
keywords: Arachidonic acid; Carbon isotope labeling; Micro-scale culture; Mortierella alpina