title: Planar Diamond-Based Multiarrays to Monitor Neurotransmitter Release and Action Potential Firing: New Perspectives in Cellular Neuroscience.
abstract: High biocompatibility, outstanding electrochemical responsiveness, inertness, and transparency make diamond-based multiarrays (DBMs) first-rate biosensors for in vitro detection of electrochemical and electrical signals from excitable cells together, with potential for in vivo applications as neural interfaces and prostheses. Here, we will review the electrochemical and physical properties of various DBMs and how these devices have been employed for recording released neurotransmitter molecules and all-or-none action potentials from living cells. Specifically, we will overview how DBMs can resolve localized exocytotic events from subcellular compartments using high-density microelectrode arrays (MEAs), or monitoring oxidizable neurotransmitter release from populations of cells in culture and tissue slices using low-density MEAs. Interfacing DBMs with excitable cells is currently leading to the promising opportunity of recording electrical signals as well as creating neuronal interfaces through the same device. Given the recent increasingly growing development of newly available DBMs of various geometries to monitor electrical activity and neurotransmitter release in a variety of excitable and neuronal tissues, the discussion will be limited to planar DBMs.
authors.affiliation: Consorzio Nazionale Interuniversitario per le Scienze fisiche della Materia (CNISM) , 10125 Torino Unit, Italy.
authors.forename: Valentina
authors.identifier: 0000-0002-8718-1208
authors.initials: V
authors.lastname: Carabelli
authors.affiliation: Consorzio Nazionale Interuniversitario per le Scienze fisiche della Materia (CNISM) , 10125 Torino Unit, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Marcantoni
authors.affiliation: Consorzio Nazionale Interuniversitario per le Scienze fisiche della Materia (CNISM) , 10125 Torino Unit, Italy.|Istituto Nazionale di Fisica Nucleare (INFN) , 10125 sez. Torino, Italy.
authors.forename: Federico
authors.initials: F
authors.lastname: Picollo
authors.affiliation: Consorzio Nazionale Interuniversitario per le Scienze fisiche della Materia (CNISM) , 10125 Torino Unit, Italy.|Istituto Nazionale di Fisica Nucleare (INFN) , 10125 sez. Torino, Italy.
authors.forename: Alfio
authors.initials: A
authors.lastname: Battiato
authors.affiliation: Consorzio Nazionale Interuniversitario per le Scienze fisiche della Materia (CNISM) , 10125 Torino Unit, Italy.|Istituto Nazionale di Fisica Nucleare (INFN) , 10125 sez. Torino, Italy.
authors.forename: Ettore
authors.initials: E
authors.lastname: Bernardi
authors.affiliation: Institute of Electron Devices and Circuits, Ulm University , 89081 Ulm, Germany.
authors.forename: Alberto
authors.initials: A
authors.lastname: Pasquarelli
authors.affiliation: Consorzio Nazionale Interuniversitario per le Scienze fisiche della Materia (CNISM) , 10125 Torino Unit, Italy.|Istituto Nazionale di Fisica Nucleare (INFN) , 10125 sez. Torino, Italy.
authors.forename: Paolo
authors.identifier: 0000-0002-7512-6295
authors.initials: P
authors.lastname: Olivero
authors.affiliation: Consorzio Nazionale Interuniversitario per le Scienze fisiche della Materia (CNISM) , 10125 Torino Unit, Italy.
authors.forename: Emilio
authors.initials: E
authors.lastname: Carbone
mesh_terms: D000200:Action Potentials / Q000502:physiology*; D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation* / Q000379:methods; D019439:Chromaffin Cells; D018130:Diamond / Q000737:chemistry*; D009474:Neurons / Q000502:physiology*; D018377:Neurotransmitter Agents / Q000378:metabolism*
chemical_list: D018377:Neurotransmitter Agents; D018130:Diamond
keywords: Diamond; action potential firing; amperometry; cell firing; chromaffin cells; electrochemical imaging; exocytosis; multiarrays; quantal release