title: The Effect of Pelargonium endlicherianum Fenzl. root extracts on formation of nanoparticles and their antimicrobial activities.
abstract: Herein, we report the biosynthesis of Ag NPs, for the first time, using identified antimicrobial molecules (gallic acid+apocynin) and (gallic acid+apocynin+quercetin) from the medicinal plant Pelargonium endlicherianum Fenzl. and dramatically enhanced antimicrobial activity. We also investigate the role of each molecule on formation Ag NPs and explain the increase in the antimicrobial activity of identified molecules mediated Ag NPs. The extraction protocols, 11% ethanol and 70% methanol, resulted in identification of different constituents of gallic acid+apocynin (M1) and gallic acid+apocynin+quercetin (M2) with respective concentrations. The M1-Ag and M2-Ag NPs exhibit excellent inhibitory activities towards Gram negative bacteria; Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 and Gram positive bacteria; Staphylococcus epidermidis ATCC 3699 bacterial using in vitro microdilution method. The minimum inhibitory concentration (MIC) values of M1-Ag and M2-Ag NPs were determined to be 7.81 and 6.25ppm for S. epidermidis, respectively. Surprisingly, MIC value for both Ag NPs was indicated to be identical as 9. 37ppm for P. aeruginosa and E., coli.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey.
authors.forename: Gökçe
authors.initials: G
authors.lastname: Şeker Karatoprak
authors.affiliation: Nanotechnology Research Center, Erciyes University, Kayseri, 38039 Turkey.
authors.forename: Gamze
authors.initials: G
authors.lastname: Aydin
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey.
authors.forename: Berrak
authors.initials: B
authors.lastname: Altinsoy
authors.affiliation: Nanotechnology Research Center, Erciyes University, Kayseri, 38039 Turkey; Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey.
authors.forename: Cevahir
authors.initials: C
authors.lastname: Altinkaynak
authors.affiliation: Department of Pharmacognosy,Faculty of Pharmacy, Eastern Mediterranean University, Gazimağusa, Mersin 10, Turkey.
authors.forename: Müberra
authors.initials: M
authors.lastname: Koşar
authors.affiliation: Nanotechnology Research Center, Erciyes University, Kayseri, 38039 Turkey; Department of Analytical Chemistry, Faculty of Pharmacy, Erciyes University, 38039 Kayseri, Turkey. Electronic address: ismailocsoy66@gmail.com.
authors.forename: Ismail
authors.initials: I
authors.lastname: Ocsoy
mesh_terms: D000098:Acetophenones / Q000302:isolation & purification / Q000494:pharmacology; D000890:Anti-Infective Agents / Q000302:isolation & purification / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D005707:Gallic Acid / Q000302:isolation & purification / Q000494:pharmacology; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008826:Microbial Sensitivity Tests; D036103:Nanotechnology; D031316:Pelargonium / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D018517:Plant Roots / Q000737:chemistry; D010946:Plants, Medicinal / Q000737:chemistry; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D011794:Quercetin / Q000302:isolation & purification / Q000494:pharmacology; D012834:Silver; D013212:Staphylococcus epidermidis / Q000187:drug effects
chemical_list: D000098:Acetophenones; D000890:Anti-Infective Agents; D010936:Plant Extracts; D012834:Silver; D005707:Gallic Acid; D011794:Quercetin; C056165:acetovanillone
keywords: Antimicrobial activity; Pelargonium endlicherianum; Silver nanoparticle