title: Biodegradable yolk-shell microspheres for ultrasound/MR dual-modality imaging and controlled drug delivery.
abstract: A new class of uniform biodegradable yolk-shell Fe3O4@PFH@PMAA-DOX microspheres was developed as ultrasound/MR dual-modality imaging contrast agents and drug delivery system. PMAA shells exhibit excellent drug loading capability and efficiency, the DOX-loading capacity is 15.4wt% and the drug loading efficiency is 91%. In vivo ultrasound and MRI experiments indicate that the as-prepared Fe3O4@PFH@PMAA-DOX microspheres successfully reach tumor tissues and possess robust dual-modality imaging capability, the r2value of MRI is 71.82mM-1s-1. In vitro cytotoxicity and in vivo tissues biopsies show that the loaded DOX can be control-released under pH, redox and ultrasound multi-stimuli and kill cancer cells effectively. Furthermore, disulfide-crosslinked PMAA shells and PGA-stabilized Fe3O4cores can be respectively degraded into short hydrophilic polymer chains and iron ions, reducing their cumulative toxicity in organisms. Thus, the multifunctional biodegradable microspheres are safer for normal tissues and more beneficial in actual clinical applications.
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China; Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States.
authors.forename: Peng
authors.initials: P
authors.lastname: Yang
authors.affiliation: Department of Radiology, Ruijin Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200025, China.
authors.forename: Xianfu
authors.initials: X
authors.lastname: Luo
authors.affiliation: Department of Colorectal Surgery, Shanghai Cancer Center, Fudan University, Shanghai 200032, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China.
authors.forename: Fang
authors.initials: F
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, United States.
authors.forename: Chuanbing
authors.initials: C
authors.lastname: Tang
authors.affiliation: State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, and Laboratory of Advanced Materials, Fudan University, Shanghai, 200433, People's Republic of China. Electronic address: ccwang@fudan.edu.cn.
authors.forename: Changchun
authors.initials: C
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000008:administration & dosage; D001672:Biocompatible Materials / Q000737:chemistry*; D001706:Biopsy; D002138:Calibration; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry*; D004317:Doxorubicin / Q000008:administration & dosage*; D004337:Drug Carriers / Q000737:chemistry*; D052203:Ferrosoferric Oxide / Q000737:chemistry; D005466:Fluorocarbons; D057809:HEK293 Cells; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008279:Magnetic Resonance Imaging; D051379:Mice; D008819:Mice, Nude; D046529:Microscopy, Electron, Transmission; D008863:Microspheres; D009369:Neoplasms / Q000188:drug therapy; D010084:Oxidation-Reduction; D010190:Pancreatic Neoplasms / Q000188:drug therapy; D011108:Polymers / Q000737:chemistry; D019904:Polymethyl Methacrylate / Q000737:chemistry*; D014463:Ultrasonography
chemical_list: D000970:Antineoplastic Agents; D001672:Biocompatible Materials; D003287:Contrast Media; D004337:Drug Carriers; D005466:Fluorocarbons; D011108:Polymers; D004317:Doxorubicin; D019904:Polymethyl Methacrylate; C078626:perflexane; D052203:Ferrosoferric Oxide
keywords: Controlled drug delivery; Dual-modality; Magnetic resonance imaging (MRI); Ultrasound imaging; Yolk-shell structure