title: Glycation of human γB-crystallin: A biophysical investigation.
abstract: Glycation of ocular lens proteins plays a vital role in the development of diabetic cataract. In order to investigate the role of glycation in cataractogenesis, the extent of glycation of human γB-crystallin was determined by an in vitro glycation study in a solution of high glucose content for upto 28days. The glycated protein has been purified and the formation of advanced glycation end products (AGEs) has been monitored spectroscopically. Size exclusion chromatographic studies showed that the covalent intermolecular crosslinking in the dimer formed was not due to disulfide bond formation. MALDI-TOF spectroscopy was employed to determine the number of glucose moieties attached to the protein due to glycation.
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Susmitnarayan
authors.initials: S
authors.lastname: Chaudhury
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Pooja
authors.initials: P
authors.lastname: Ghosh
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
authors.forename: Sultana
authors.initials: S
authors.lastname: Parveen
authors.affiliation: Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur 721302, India. Electronic address: swagata@chem.iitkgp.ernet.in.
authors.forename: Swagata
authors.initials: S
authors.lastname: Dasgupta
mesh_terms: D055592:Biophysical Phenomena; D005947:Glucose / Q000378:metabolism; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006031:Glycosylation; D006801:Humans; D055503:Protein Multimerization; D020836:Protein Structure, Quaternary; D017433:Protein Structure, Secondary; D038222:gamma-Crystallins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D017127:Glycation End Products, Advanced; C107738:crystallin, gammaB; D038222:gamma-Crystallins; D005947:Glucose
keywords: Diabetic cataract; Glycation; Human γB-crystallin