title: Metabolomics study of Populus type propolis.
abstract: Herein, we propose rapid and simple spectroscopic methods to determine the chemical composition of propolis derived from various Populus species using a metabolomics approach. In order to correlate variability in Populus type propolis composition with the altitude of its collection, NMR, IR, and UV spectroscopy followed by OPLS was conducted. The botanical origin of propolis was established by comparing propolis spectral data to those of buds of various Populus species. An O2PLS method was utilized to integrate two blocks of data. According to OPLS and O2PLS, the major compounds in propolis samples, collected from temperate continental climate above 500m, were phenolic glycerides originating from P. tremula buds. Flavonoids were predominant in propolis samples collected below 400m, originating from P. nigra and P. x euramericana buds. Samples collected at 400-500m were of mixed origin, with variable amounts of all detected metabolites.
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Boban
authors.initials: B
authors.lastname: Anđelković
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Ljubodrag
authors.initials: L
authors.lastname: Vujisić
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Ivan
authors.initials: I
authors.lastname: Vučković
authors.affiliation: Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Vele
authors.initials: V
authors.lastname: Tešević
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
authors.forename: Vlatka
authors.initials: V
authors.lastname: Vajs
authors.affiliation: Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia. Electronic address: dgodjev@chem.bg.ac.rs.
authors.forename: Dejan
authors.initials: D
authors.lastname: Gođevac
mesh_terms: D000818:Animals; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055432:Metabolomics / Q000379:methods*; D015999:Multivariate Analysis; D032107:Populus / Q000737:chemistry / Q000378:metabolism*; D011429:Propolis / Q000032:analysis / Q000378:metabolism*
chemical_list: D011429:Propolis
keywords: IR; NMR; O2PLS; OPLS; Propolis; UV