title: Bacterial community profile of contaminated soils in a typical antimony mining site.
abstract: The soils around the world's largest antimony mine have been contaminated by high concentrations of Sb and As, which might influence microbial diversity in the surrounding soils. The ecological effects of bioavailable Sb and As on the composition and diversity of microbial community in soils remain unknown. In this study, the relative abundance, taxonomic diversity and composition of bacterial community in soils from a typical Sb mine area, and the relationship between the bacterial community and bioavailable concentrations as well as environmental factors have been investigated comprehensively using high-throughput sequencing (HTS) and diffusive gradients in thin films (DGT). The results indicated that Proteobacteria, Acidobacteria, Chloroflexi, Bacteroidetes, Actinobacteria, Gemmatimonadetes, and Cyanobacteria were the dominant bacterial populations at phylum level in all soil samples, accounting for more than 80% of the bacteria sequenced. The abundance and diversity of bacterial community vary along a metal contamination gradient. Redundancy discriminate analysis (RDA) revealed that 74.74% of bacterial community variation in the contaminated soils was explained by six environmental factors (pH, SbDGT, AsDGT, potential ecological risk index (RI), TC, TN), among which pH, SbDGT, and AsDGTwere dominant factors influencing the composition and diversity of bacteria. This study contributes to our understanding of microbial diversity in a local ecosystem and introduces the option of studying bioavailable Sb and As using DGT.
authors.affiliation: State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, People's Republic of China.
authors.forename: Ningning
authors.initials: N
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, People's Republic of China.
authors.forename: Suhuan
authors.initials: S
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Water Environment Simulation, School of Environment, Beijing Normal University, Beijing, 100875, People's Republic of China. hemc@bnu.edu.cn.
authors.forename: Mengchang
authors.identifier: http://orcid.org/0000-0001-8975-8034
authors.initials: M
authors.lastname: He
mesh_terms: D000965:Antimony / Q000032:analysis*; D001419:Bacteria / Q000145:classification* / Q000235:genetics; D044822:Biodiversity; D002681:China; D008906:Mining; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012987:Soil / Q000737:chemistry*; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000032:analysis*
chemical_list: D012336:RNA, Ribosomal, 16S; D012987:Soil; D012989:Soil Pollutants; D000965:Antimony
keywords: Antimony contamination; Bacterial community profiling; Diffusive gradients in thin films (DGT); High-throughput sequencing; Typical antimony-mining site