title: Characterization of goat inner cell mass derived cells in double kinase inhibition condition.
abstract: The identification of small molecular inhibitors, which were reported to promote the derivation of mouse and human embryonic stem cells (ESCs), provides a potential strategy for the derivation of domesticated ungulate ESCs. In present study, goat inner cell mass (ICM) derived cells in the double inhibition (2i) condition, in which, mitogen-activated protein kinase kinase (MAP2K) and glycogen synthase kinase 3 (GSK3) were inhibited by PD0325901 and BIO respectively, were characterized. The results showed that goat ICM derived cells in 2i medium adding leukaemia inhibitor factor (LIF) possessed a mouse ES-like morphology. But these cells had much compromised proliferation capacity, resulting in difficulty in expansion. In 2i alone medium, goat ICM derived cells possessed primate ES-like morphology. These cells expressed pluripotent markers and could differentiate into derivatives of three germ layers in vitro. However, these cells could not be proliferated in long-term (persisted for 15 passages) because of spontaneously neural differentiation. Additionally, goat ICM derived cells could be inducing differentiated into neural lineage in vitro. Although goat ESCs could not be established in PD0325901 and BIO alone medium, this derivation condition provides a useful research system to find signaling molecular those regulate early embryonic development and pluripotency in goat.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Xi
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Xiaokun
authors.initials: X
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Kai
authors.initials: K
authors.lastname: Meng
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, 712100, People's Republic of China.
authors.forename: Xiaoe
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, 712100, People's Republic of China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Yangling, Shaanxi, 712100, People's Republic of China. Electronic address: mabh@nwsuaf.edu.cn.
authors.forename: Baohua
authors.initials: B
authors.lastname: Ma
mesh_terms: D000818:Animals; D001549:Benzamides / Q000737:chemistry; D001755:Blastocyst / Q000378:metabolism; D002454:Cell Differentiation; D019070:Cell Lineage; D049109:Cell Proliferation; D018920:Coculture Techniques; D004159:Diphenylamine / Q000031:analogs & derivatives / Q000737:chemistry; D053595:Embryonic Stem Cells / Q000166:cytology*; D005347:Fibroblasts / Q000378:metabolism; D005855:Germ Layers; D038362:Glycogen Synthase Kinase 3 / Q000378:metabolism; D006041:Goats; D053605:Leukemia Inhibitory Factor / Q000378:metabolism; D051379:Mice; D009474:Neurons / Q000378:metabolism*; D039904:Pluripotent Stem Cells / Q000166:cytology*; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction
chemical_list: D001549:Benzamides; D053605:Leukemia Inhibitory Factor; C506614:mirdametinib; D004159:Diphenylamine; D038362:Glycogen Synthase Kinase 3
keywords: BIO; Embryonic stem cell; Goat; Inner cell mass; PD0325901