title: The Interaction of Antimicrobial Peptides with the Membrane and Intracellular Targets of Staphylococcus aureus Investigated by ATP Leakage, DNA-Binding Analysis, and the Expression of a LexA-Controlled Gene, recA.
abstract: The analysis of how antimicrobial peptides (AMPs) interact with bacterial membranes and intracellular targets is important for our understanding of how these molecules affect bacteria. Increased knowledge may aid the design of AMPs that work on their target bacterium without inducing bacterial resistance. Here, we describe different methods to investigate the mode of action of peptides against the Gram-positive bacterium Staphylococcus aureus. ATP leakage analysis can be used to evaluate the ability of AMPs to perturb bacteria. DNA-binding and SOS response induction can be analyzed to investigate intracellular targets.
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, Frederiksberg, 1870, Denmark.
authors.forename: Sanne
authors.initials: S
authors.lastname: Gottschalk
authors.affiliation: Department of Veterinary Disease Biology, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, Frederiksberg, 1870, Denmark. leth@sund.ku.dk.
authors.forename: Line Elnif
authors.initials: LE
authors.lastname: Thomsen
mesh_terms: D000255:Adenosine Triphosphate / Q000378:metabolism*; D000900:Anti-Bacterial Agents / Q000378:metabolism* / Q000494:pharmacology; D023181:Antimicrobial Cationic Peptides / Q000378:metabolism* / Q000494:pharmacology; D001426:Bacterial Proteins / Q000378:metabolism; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism*; D002463:Cell Membrane Permeability / Q000187:drug effects; D004247:DNA / Q000378:metabolism*; D015964:Gene Expression Regulation, Bacterial / Q000187:drug effects; D011485:Protein Binding; D011938:Rec A Recombinases / Q000235:genetics; D013014:SOS Response, Genetics; D012697:Serine Endopeptidases / Q000378:metabolism; D013211:Staphylococcus aureus / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism*; D000099160:LexA Repressor Protein
chemical_list: D000255:Adenosine Triphosphate; D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D001426:Bacterial Proteins; D004247:DNA; D011938:Rec A Recombinases; D012697:Serine Endopeptidases; D000099160:LexA Repressor Protein
keywords: ATP leakage; Antimicrobial peptides; DNA binding; Membrane perturbation; Mode of action; SOS response