title: Effects of tamoxifen-loaded solid lipid nanoparticles on the estrogen receptor-α (ER-α) and vascular endothelial growth factor-A (VEGF-A) genes expression in the endometrial tissue of ovariectomized female Sprague-Dawley rats.
abstract: BACKGROUND
  The effect of tamoxifen on endometrial carcinogenesis stems from its estrogen agonist effect. An in vivo study was carried out to compare the effect of tamoxifen-loaded solid lipid nanoparticles and free drug on the ER-α and VEGF-A genes expression.
  
  
  METHODS
  Twenty-four female Sprague-Dawley rats divided into 4 groups of six rats were used for this study. The first and second groups were ovariectomized and given tamoxifen and tamoxifen-loaded SLN respectively for six days continuously. Group 3 served as the untreated ovariectomized control group and group 4 was made up of untreated normal healthy rats. At the end of the study, the rats were sacrificed and study of the genes expression and serum oxidative stress were carried out.
  
  
  RESULTS
  The results of this study showed that treatment with tamoxifen-loaded SLN significantly reduced the mRNA levels of ERα and VEGF-A gene and the total oxidant status compared to the ovariectomized control group.
  
  
  CONCLUSIONS
  The results of this study revealed that encapsulation of tamoxifen in solid lipid nanoparticles may have less adverse effects on the oxidative stress status and incidence of endometrial cancer.
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Saman
authors.initials: S
authors.lastname: Javid
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Nasrin
authors.initials: N
authors.lastname: Ziamajidi
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
authors.forename: Shadi
authors.initials: S
authors.lastname: Foroughi
authors.affiliation: Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran. Electronic address: rpourkabir@hotmail.com.
authors.forename: Roghayeh
authors.initials: R
authors.lastname: Abbasalipourkabir
mesh_terms: D000818:Animals; D001835:Body Weight / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry; D004717:Endometrium / Q000187:drug effects* / Q000378:metabolism; D047628:Estrogen Receptor alpha / Q000235:genetics*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D008055:Lipids / Q000737:chemistry*; D053758:Nanoparticles / Q000737:chemistry*; D010052:Ovariectomy; D018384:Oxidative Stress / Q000187:drug effects; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017207:Rats, Sprague-Dawley; D013629:Tamoxifen / Q000737:chemistry / Q000494:pharmacology*; D042461:Vascular Endothelial Growth Factor A / Q000235:genetics*
chemical_list: D004337:Drug Carriers; D047628:Estrogen Receptor alpha; D008055:Lipids; D012333:RNA, Messenger; D042461:Vascular Endothelial Growth Factor A; D013629:Tamoxifen
keywords: ERα; Tamoxifen; VEGF-A