title: Production of FucoPol by Enterobacter A47 using waste tomato paste by-product as sole carbon source.
abstract: Out-of-specification tomato paste, a by-product from the tomato processing industry, was used as the sole substrate for cultivation of the bacterium Enterobacter A47 and production of FucoPol, a value-added fucose-rich extracellular polysaccharide. Among the different tested fed-batch strategies, pH-stat, DO-stat and continuous substrate feeding, the highest production (8.77gL-1) and overall volumetric productivity (2.92gL-1d-1) were obtained with continuous substrate feeding at a constant flow rate of 11gh-1. The polymer produced had the typical FucoPol composition (37mol% fucose, 27mol% galactose, 23mol% glucose and 12mol% glucuronic acid, with an acyl groups content of 13wt%). The average molecular weight was 4.4×106Da and the polydispersity index was 1.2. This study demonstrated that out-of-specification tomato paste is a suitable low-cost substrate for the production of FucoPol, thus providing a route for the valorization of this by-product into a high-value microbial product.
authors.affiliation: UCIBIO-REQUIMTE, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Sílvia
authors.initials: S
authors.lastname: Antunes
authors.affiliation: UCIBIO-REQUIMTE, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
authors.forename: Filomena
authors.initials: F
authors.lastname: Freitas
authors.affiliation: Interfacultary Research Centre of Biomaterials (CEIB), University of Liège, B-4000 Liège, Belgium.
authors.forename: Chantal
authors.initials: C
authors.lastname: Sevrin
authors.affiliation: Interfacultary Research Centre of Biomaterials (CEIB), University of Liège, B-4000 Liège, Belgium.
authors.forename: Christian
authors.initials: C
authors.lastname: Grandfils
authors.affiliation: UCIBIO-REQUIMTE, Chemistry Department, FCT/Universidade Nova de Lisboa, 2829-516 Caparica, Portugal. Electronic address: amr@fct.unl.pt.
authors.forename: Maria A M
authors.initials: MA
authors.lastname: Reis
mesh_terms: D000383:Agriculture; D004754:Enterobacter / Q000737:chemistry / Q000378:metabolism*; D018551:Solanum lycopersicum / Q000737:chemistry*; D011135:Polysaccharides, Bacterial / Q000737:chemistry; D014866:Waste Products
chemical_list: D011135:Polysaccharides, Bacterial; D014866:Waste Products
keywords: Continuous feeding; DO-stat mode; Extracellular polysaccharide (EPS); Tomato paste; pH-stat mode