title: Isolation, Screening, and Identification of Novel Isolates of Actinomycetes from India for Antimicrobial Applications.
abstract: The search for novel bioactive compounds from the natural environment has rapidly been gaining momentum with the increase in multi-drug resistant (MDR) pathogens. In the present study, the antimicrobial potential of novel actinomycetes has been evaluated by initial screening of six soil samples. Primary and secondary screening was performed againstBacillus subtilis, Staphylococcus aureus, Escherichia coli, Candida albicans, Candida tropicalis, Trichophyton rubrum, and other MDR bacterial and fungal test strains, thirteen active isolates were selected for further study. Microbial strains were identified on the basis of growth conditions and other biochemical characters. Five most active microbial strains were identified using 16S rRNA sequence homology and designated asStreptomyces xanthophaeusMTCC 11938,Streptomyces variabilisMTCC 12266,Streptomyces xanthochromogenesMTCC 11937,Streptomyces levisEU 124569, andStreptomycessp. NCIM 5500. Four antibacterial and three antifungal compounds isolated from the above five isolates were purified and partially characterized using UV absorption and IR spectra. Two antibacterial metabolites, belong to chromone and peptide antibiotic, respectively. The antifungal compounds were found to be of non-polyene nature. In conclusion, we study the isolation of novel bacterial strains of actinomycetes for producing novel compounds having antibacterial and antifungal activities from the unexplored agro-ecological niches of India. Also, this study paves the way for further characterization of these isolates ofStreptomycessp. for their optimum utilization for antimicrobial purposes.
authors.affiliation: Microbiology Division, Council of Scientific and Industrial Research-Central Drug Research InstituteLucknow, India; Department of Biotechnology, Institute of Engineering and TechnologyLucknow, India.
authors.forename: Vineeta
authors.initials: V
authors.lastname: Singh
authors.affiliation: Department of Biosciences, Jamia Millia Islamia (A Central University)New Delhi, India; Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan UniversityJazan, Saudi Arabia.
authors.forename: Shafiul
authors.initials: S
authors.lastname: Haque
authors.affiliation: Microbiology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Harshita
authors.initials: H
authors.lastname: Singh
authors.affiliation: Microbiology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Jyoti
authors.initials: J
authors.lastname: Verma
authors.affiliation: Microbiology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Kumari
authors.initials: K
authors.lastname: Vibha
authors.affiliation: Fermentation Technology Division, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Rajbir
authors.initials: R
authors.lastname: Singh
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan UniversityJazan, Saudi Arabia; Department of Biotechnology, Himachal Pradesh UniversityShimla, India.
authors.forename: Arshad
authors.initials: A
authors.lastname: Jawed
authors.affiliation: Research and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan UniversityJazan, Saudi Arabia; Department of Biotechnology, Shri Ramswaroop Memorial UniversityLucknow, India.
authors.forename: C K M
authors.initials: CK
authors.lastname: Tripathi
keywords: Streptomyces sp.; antibiotic production; antimicrobial activity; chromone antibiotics; non-polyenes