title: A lignocellulosic hydrolysate-tolerant Aurantiochytrium sp. mutant strain for docosahexaenoic acid production.
abstract: To utilize lignocellulosic hydrolysate for docosahexaenoic acid (DHA) production, a novel mutant Aurantiochytrium sp. FN21 with strong tolerance against inhibitory lignocellulosic hydrolysate was obtained through continuous domestication processes from the parent strain Aurantiochytrium sp. FJU-512. Aurantiochytrium sp. FN21 can accumulate 21.3% and 30.7% more DHA compared to its parent strain cultured in fermentation medium and a medium with 50% (v/v) sugarcane bagasse hydrolysate (SBH), respectively. After optimization with different nitrogen sources, the highest lipid (11.84g/L) and DHA (3.15g/L) production were achieved in SBH. The results demonstrated that Aurantiochytrium sp. FN21 has the commercial applications for DHA production using lignocellulosic hydrolysate. In order to elucidate the tolerance mechanism, transcriptomic profiling of the two strains was studied. The highly up-regulated genes and corresponding cellular pathways (TCA cycle, amino acid biosynthesis, fatty acid metabolism and degradation of aromatic compounds) are considered to be associated with the hydrolysate-tolerance of Aurantiochytrium sp. FN21.
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Qi
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
authors.forename: Mingliang
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
authors.forename: Youwei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
authors.forename: Xianzhang
authors.initials: X
authors.lastname: Jiang
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China.
authors.forename: Jinxin
authors.initials: J
authors.lastname: Lin
authors.affiliation: Fujian Vocational College of Bio-engineering, Fuzhou, Fujian 350002, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Cao
authors.affiliation: Engineering Research Center of Industrial Microbiology of Ministry of Education, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China. Electronic address: hjz@fjnu.edu.cn.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Huang
mesh_terms: D000222:Adaptation, Physiological; D018533:Biomass; D004281:Docosahexaenoic Acids / Q000096:biosynthesis*; D005227:Fatty Acids / Q000378:metabolism; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D050356:Lipid Metabolism / Q000235:genetics; D009154:Mutation / Q000235:genetics*; D058009:Stramenopiles / Q000235:genetics / Q000302:isolation & purification / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; C036909:lignocellulose; D004281:Docosahexaenoic Acids; D008031:Lignin
keywords: Aurantiochytrium sp. FN21; Hydrolysate; Lipid; Transcriptome