title: IL-3 and GM-CSF modulate functions of splenic macrophages in ENU induced leukemia.
abstract: Leukemia is often accompanied by enhanced susceptibility to infection due to compromised B cell and T cell functions. The alterations in macrophage functions in leukemia remain less investigated. Herein, we examined macrophages for their functions and responsiveness to IL-3 and GM-CSF in ENU-induced leukemia in BALB/c mice. We observed that the macrophages from the leukemic mice had less phagocytic activity, reduced chemotactic activity in response to monocyte chemoattractant protein-1(MCP-1), and decreased expression of iNOS, GM-CSF, TLR2, TLR4, IFN-γ and TNF-α. These impaired macrophage functions in leukemic mice were significantly corrected by IL-3 and GM-CSF treatment indicating the therapeutic benefit of these two cytokines in leukemia.
authors.affiliation: Immunology Microbiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura 799022, India.
authors.forename: Ashish Kumar
authors.initials: AK
authors.lastname: Singha
authors.affiliation: Immunology Microbiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura 799022, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Bhattacharjee
authors.affiliation: National Centre for Cell Science, Ganeshkhind, Pune 411 007, India.
authors.forename: Bhaskar
authors.initials: B
authors.lastname: Saha
authors.affiliation: Immunology Microbiology Lab, Department of Human Physiology, Tripura University, Suryamaninagar, Tripura 799022, India. Electronic address: debasish.maiti@tripurauniv.in.
authors.forename: Debasish
authors.initials: D
authors.lastname: Maiti
mesh_terms: D000818:Animals; D005038:Ethylnitrosourea / Q000633:toxicity*; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000276:immunology / Q000494:pharmacology*; D007377:Interleukin-3 / Q000276:immunology / Q000494:pharmacology*; D007938:Leukemia / Q000139:chemically induced / Q000188:drug therapy / Q000276:immunology / Q000473:pathology; D008264:Macrophages / Q000276:immunology* / Q000473:pathology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D009363:Neoplasm Proteins / Q000276:immunology*; D013154:Spleen / Q000276:immunology* / Q000473:pathology
chemical_list: D007377:Interleukin-3; D009363:Neoplasm Proteins; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D005038:Ethylnitrosourea
keywords: Chemotaxis; Granulocyte macrophage-colony stimulating factor; IFN-γ; Interleukin-3; Leukemia; Monocyte-derived macrophages; Nitric oxide synthase 2; Phagocytosis; TLRs