title: Soluble tumor necrosis factor receptor I (sTNFRI) as a prognostic factor in melanoma patients in Slovene population.
abstract: Tumor necrosis factor α (TNF-α) and its receptors (TNFRI and TNFRII) which exist in soluble form as a product of cleavage of the extracellular domain of membrane integrated receptors, still rise debate about their importance. It was reported that TNF-α has numerous actions in diseases such as inflammation, autoimmunity, infectious diseases, septic shock and many types of cancer [1, 2]. Several authors have reported the significance of sTNFRI level in serum of cancer patients [3, 4]. This study was performed in collaboration with the Institute of Oncology of Slovenia.At least two different mouse monoclonal antibodies (MAbs) against human sTNFRI have been prepared to obtain a sensitive and reliable sandwich ELISA. It was compared with commercially available R&D and Endogen ELISAs for the determination of sTNFRI. Groups of patients with different stages of melanoma and epithelial ovarian carcinoma were tested and their clinical records were reexamined. Levels of sTNFRI were measured and compared with the normal serum levels of sTNFRI.
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Vesna
authors.initials: V
authors.lastname: Galvani
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Katrina Pretnar
authors.initials: KP
authors.lastname: Hartman
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Ruth R
authors.initials: RR
authors.lastname: Rupreht
authors.affiliation: Institute of Oncology, Zaloška 2, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Srdjan
authors.initials: S
authors.lastname: Novaković
authors.affiliation: Institute of Oncology, Zaloška 2, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Borut
authors.initials: B
authors.lastname: Štabuc
authors.affiliation: Institute of Oncology, Zaloška 2, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Janja
authors.initials: J
authors.lastname: Ocvirk
authors.affiliation: Lek d.d., Celovška 135, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Viktor
authors.initials: V
authors.lastname: Menart
authors.affiliation: National Institute of Chemistry, Hajdrihova 19, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Vladimira Gaberc
authors.initials: VG
authors.lastname: Porekar
authors.affiliation: Lek d.d., Celovška 135, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Anton
authors.initials: A
authors.lastname: Štalc
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Primož
authors.initials: P
authors.lastname: Rožman
authors.affiliation: Blood Transfusion Centre of Slovenia, Šlajmerjeva 6, 1000 Ljubljana, Slovenia, Slovenia.
authors.forename: Vladka Čurin
authors.initials: VČ
authors.lastname: Šerbec
keywords: ELISA; Key words sTNFRI; melanoma; ovarian carcinoma