title: Tranexamic acid effects on cartilage and synovial tissue: an in vitro study for a possible safe intra-articular use.
abstract: The possible toxic effects of intra-articular tranexamic acid (TA) are still debated. The aim of this study was to evaluate TA effects on human cartilage fragments and synovial biopsies. Explant culture of minced articular cartilage underwent prolonged TA exposure. Histological analysis, immunofluorescence and colorimetric assay for quantification of s-GAG and DNA were performed at the end term. Synoviocytes were cultured for 48h in presence of TA. Light microscopy and flow cytometry analysis were performed at the end of the exposure to TA and one week after the treatment. TA exposure did not influence i) the chondrocyte outgrowth and migration, ii) the expression of chondrogenic and proliferative markers and iii) the s-GAG/DNA ratio. TA treatment did not affect synoviocytes’ morphology and treated cells were phenotypically similar to control cells. This study demonstrated that TA does not negatively affect chondrocytes and synoviocytes cultured in vitro. Thus, our findings may be clinically relevant in order to validate the intra-articular TA administration during orthopedic procedures.
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.|Molecular Biotechnology Center, University of Turin, Turin, Italy
authors.forename: A
authors.initials: A
authors.lastname: Marmotti
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.|Molecular Biotechnology Center, University of Turin, Turin, Italy
authors.forename: S
authors.initials: S
authors.lastname: Mattia
authors.affiliation: IRCCS Istituto Ortopedico Galeazzi, Milan, Italy
authors.forename: L
authors.initials: L
authors.lastname: Mangiavini
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
authors.forename: D E
authors.initials: DE
authors.lastname: Bonasia
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Bruzzone
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Dettoni
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Rosso
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Blonna
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
authors.forename: R
authors.initials: R
authors.lastname: Rossi
authors.affiliation: Department of Orthopaedics and Traumatology, University of Turin, Turin, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Castoldi
authors.affiliation: IRCCS Istituto Ortopedico Galeazzi, Milan, Italy|Department of Biomedical Sciences for Health, University of Milan, Milan, Italy
authors.forename: G M
authors.initials: GM
authors.lastname: Peretti
mesh_terms: D002358:Cartilage, Articular / Q000166:cytology / Q000187:drug effects*; D002478:Cells, Cultured; D019902:Chondrocytes / Q000166:cytology / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects; D006801:Humans; D000070918:Synoviocytes / Q000166:cytology / Q000187:drug effects; D014148:Tranexamic Acid / Q000009:adverse effects / Q000494:pharmacology*
chemical_list: D014148:Tranexamic Acid