title: Effects of chronic restraint-induced stress on radiation-induced chromosomal aberrations in mouse splenocytes.
abstract: Both ionizing radiation (IR) and psychological stress (PS) cause detrimental effects on humans. A recent study showed that chronic restraint-induced PS (CRIPS) diminished the functions of Trp53 and enhanced radiocarcinogenesis in Trp53-heterozygous (Trp53+/-) mice. These findings had a marked impact on the academic field as well as the general public, particularly among residents living in areas radioactively contaminated by nuclear accidents. In an attempt to elucidate the modifying effects of CRIPS on radiation-induced health consequences in Trp53 wild-type (Trp53+/+) animals, investigations involving multidisciplinary analyses were performed. We herein demonstrated that CRIPS induced changes in the frequency of IR-induced chromosomal aberrations (CAs) in splenocytes. Five-week-old male Trp53+/+C57BL/6J mice were restrained for 6h per day for 28 consecutive days, and total body irradiation (TBI) at a dose of 4Gy was performed on the 8th day. Metaphase chromosome spreads prepared from splenocytes at the end of the 28-day restraint regimen were painted with fluorescence in situ hybridization (FISH) probes for chromosomes 1, 2, and 3. The results obtained showed that CRIPS alone did not induce CAs, while TBI caused significant increases in CAs, mostly translocations. Translocations appeared at a lower frequency in mice exposed to TBI plus CRIPS than in those exposed to TBI alone. No significant differences were observed in the frequencies of the other types of CAs (insertions, dicentrics, and fragments) visualized with FISH between these experimental groups (TBI+CRIPS vs. TBI). These results suggest that CRIPS does not appear to synergize with the clastogenicity of IR.
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: katsube.takanori@qst.go.jp.
authors.forename: Takanori
authors.initials: T
authors.lastname: Katsube
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: wang.bing@qst.go.jp.
authors.forename: Bing
authors.initials: B
authors.lastname: Wang
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: tanaka.kaoru@qst.go.jp.
authors.forename: Kaoru
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: ninomiya.yasuharu@qst.go.jp.
authors.forename: Yasuharu
authors.initials: Y
authors.lastname: Ninomiya
authors.affiliation: Advanced Medical Instrumentation Unit, Okinawa Institute of Science and Technology Graduate University, Okinawa, 904-0495, Japan. Electronic address: guillaume.vares@oist.jp.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Varès
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: kawagoshi.taiki@qst.go.jp.
authors.forename: Taiki
authors.initials: T
authors.lastname: Kawagoshi
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: shiomi.naoko@qst.go.jp.
authors.forename: Naoko
authors.initials: N
authors.lastname: Shiomi
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: kubota.yoshihisa@qst.go.jp.
authors.forename: Yoshihisa
authors.initials: Y
authors.lastname: Kubota
authors.affiliation: Institute of Radiation Medicine, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin, 300192, PR China. Electronic address: liuqiang@irm-cams.ac.cn.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Biomedical Science and Technology, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, 770-8503, Japan. Electronic address: morita@tokushima-u.ac.jp.
authors.forename: Akinori
authors.initials: A
authors.lastname: Morita
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: nakajima.tetsuo@qst.go.jp.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nakajima
authors.affiliation: National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Chiba, 263-8555, Japan. Electronic address: nenoi.mitsuru@qst.go.jp.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Nenoi
mesh_terms: D000818:Animals; D002869:Chromosome Aberrations; D007103:Immobilization; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D013154:Spleen / Q000528:radiation effects*; D013312:Stress, Physiological; D016159:Tumor Suppressor Protein p53 / Q000235:genetics; D014916:Whole-Body Irradiation
chemical_list: D016159:Tumor Suppressor Protein p53
keywords: Chronic restraint-induced psychological stress; Fluorescence in situ hybridization (FISH); Mouse restraint model; Total body irradiation