title: attI1-Located Small Open Reading Frames ORF-17 and ORF-11 in a Class 1 Integron Affect Expression of a Gene Cassette Possessing a Canonical Shine-Dalgarno Sequence.
abstract: By searching the Integrall integron and GenBank databases, a novel open reading frame (ORF) of 51 nucleotides (nts) (ORF-17) overlapping the previously described ORF-11 was identified within theattI1site in virtually all class 1 integrons. Using a set of isogenic plasmid constructs carrying a single gene cassette (blaGES-1) and possessing a canonical translation initiation region, we found that ORF-17 contributes to GES-1 expression.
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Costas C
authors.identifier: 0000-0002-9912-9028
authors.initials: CC
authors.lastname: Papagiannitsis
authors.affiliation: Department of Microbiology, Medical School, University of Athens, Athens, Greece.
authors.forename: Leonidas S
authors.initials: LS
authors.lastname: Tzouvelekis
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece.
authors.forename: Eva
authors.initials: E
authors.lastname: Tzelepi
authors.affiliation: Laboratory of Bacteriology, Hellenic Pasteur Institute, Athens, Greece miriagou@pasteur.gr.
authors.forename: Vivi
authors.initials: V
authors.lastname: Miriagou
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D001483:Base Sequence; D018387:Codon, Initiator; D004269:DNA, Bacterial / Q000235:genetics / Q000378:metabolism; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D015870:Gene Expression; D032023:Integrons; D008826:Microbial Sensitivity Tests; D009154:Mutation; D016366:Open Reading Frames; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D014176:Protein Biosynthesis; D011550:Pseudomonas aeruginosa / Q000187:drug effects / Q000235:genetics* / Q000378:metabolism; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000235:genetics* / Q000378:metabolism; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D018387:Codon, Initiator; D004269:DNA, Bacterial; D047090:beta-Lactams; C405902:GES-1 beta-lactamase; D001618:beta-Lactamases
keywords: attI1 sequence; class 1 integrons; expression; gene cassettes