title: Tooth tissue engineering: tooth decellularization for natural scaffold.
abstract: OBJECTIVE
  Tissue engineering is a multidisciplinary science that aims to produce replacement organs and biological substitutes. One of the techniques involves decellularizing a biological organ without altering its structure. One challenge is how to demonstrate which method would be better for this process.
  
  
  METHODS
  Fifty premolar teeth were divided into five groups: G1 (control): solution of 10% formaldehyde; G2: phosphate buffer saline (PBS), 28 g of tetrasodium ethylenediaminetetraacetic (EDTA), sodium hypochlorite 2.5% (SH); G3: PBS, EDTA and 40v hydrogen peroxide (HP); G4: PBS, EDTA, SH, enzymatic detergent (ED); and G5: PBS, EDTA, HP, ED. Each group was analyzed by scanning electron microscopy (SEM), x-ray, measured weights and color and received statistical analysis.
  
  
  CONCLUSIONS
  This study demonstrated that G5 was the most appropriate method to obtain a natural scaffold.
authors.affiliation: CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil.
authors.forename: Luciana Aparecida
authors.initials: LA
authors.lastname: de Sousa Iwamoto
authors.affiliation: CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil.
authors.forename: Monica Talarico
authors.initials: MT
authors.lastname: Duailibi
authors.affiliation: Material Engineering, UNIFESP- Universidade Federal de São Paulo, Sao José dos Campos, Sao Paulo, Brazil; Material Engineering, UNIFESP- Universidade Federal de São Paulo, Sao José dos Campos, Sao Paulo, Brazil.
authors.forename: Gerson Yoshinobu
authors.initials: GY
authors.lastname: Iwamoto
authors.affiliation: Health Science Department, UNISA - Universidade de Santo Amaro, Sao Paulo, Brazil; Health Science Department, UNISA - Universidade de Santo Amaro, Sao Paulo, Brazil.
authors.forename: Yara
authors.initials: Y
authors.lastname: Juliano
authors.affiliation: Faculdade de Ciencias Medicas Santa Casa de Sao Paulo, Sao Paulo, Brazil; Faculdade de Ciencias Medicas Santa Casa de Sao Paulo, Sao Paulo, Brazil.
authors.forename: Michel Silvio
authors.initials: MS
authors.lastname: Duailibi
authors.affiliation: ESALQ- Escola Superior de Agronomia Luiz de Queiroz, Universidade de Sao Paulo, Sao Paulo, Brazil; ESALQ- Escola Superior de Agronomia Luiz de Queiroz, Universidade de Sao Paulo, Sao Paulo, Brazil.
authors.forename: Francisco André
authors.initials: FA
authors.lastname: Ossamu Tanaka
authors.affiliation: CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil; CTCMol, Center of Cellular & Molecular Therapy, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; Translational Surgery, Surgery Department, UNIFESP- Universidade Federal de Sao Paulo- Escola Paulista de Medicina, Sao Paulo, Brazil; National Institute of Science & Technology, Biofabrication Institute, BIOFABRIS, Campinas, São Paulo, Brazil.
authors.forename: Silvio Eduardo
authors.initials: SE
authors.lastname: Duailibi
keywords: decellularization; demineralization; tissue engineering; tissue scaffold