title: Changes of circulating Th22 cells in children with hand, foot, and mouth disease caused by enterovirus 71 infection.
abstract: Interleukin (IL)-22+CD4+T (Th22) cells play crucial roles in the pathogenesis of autoimmune diseases and infectious diseases, although the role of Th22 cells remains largely unclear in children with hand, foot, and mouth disease (HFMD) caused by enterovirus 71 (EV71). This study aims to explore the role of circulating IL-22+IL-17A-CD4+T (cTh22) cells in children with EV71-associated HFMD. We found that during the acute stage of illness, the frequencies of cTh22 and circulating IL-22+IL-17A+CD4+T (IL-22+cTh17) cells in CD4+T cells infrom affected patients, and especially in severely affected patients, were significantly higher than in healthy controls (HC). The major source of IL-22 production was cTh22 cells, partially from cTh17 cells. Moreover, the protein and mRNA levels of IL-22, IL-17A, IL-23, IL-6, and TNF-α were significantly different among the mild patients, severe patients and HC, as well as AHR and RORγt mRNA levels. A positive correlation was found between plasma IL-22 levels and cTh22 cell frequencies, and cTh17 cell and IL-22+ cTh17 cell frequencies. Furthermore, the frequencies of cTh22 were significantly decreased in the convalescent patients. Our findings indicated that cTh22 cells could play critical roles in the pathogenesis of EV71 infection, and are potential therapeutic targets for patients with EV71-associated HFMD.
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Cui
authors.affiliation: Department of General Surgery, the Second Affiliated Hospital of Soochow University, Suzhou, China.
authors.forename: Fengyun
authors.initials: F
authors.lastname: Zhong
authors.affiliation: Department of Clinical Laboratory, Center of Community Health Service of Qingbo Street, Hangzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lin
authors.affiliation: Clinical Laboratory, Hangzhou Children's Hospital, Hangzhou, China.
authors.forename: Yidong
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Quan
authors.initials: Q
authors.lastname: Long
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Xianzhi
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Qiaoyun
authors.initials: Q
authors.lastname: Zhu
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Li
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Qifen
authors.initials: Q
authors.lastname: Mao
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Huo
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Guoliang
authors.initials: G
authors.lastname: Xie
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Shufa
authors.initials: S
authors.lastname: Zheng
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Laboratory Medicine, First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Hangzhou, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Chen
mesh_terms: D002675:Child, Preschool; D029821:Enterovirus A, Human / Q000472:pathogenicity*; D005260:Female; D006232:Hand, Foot and Mouth Disease / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D020381:Interleukin-17 / Q000378:metabolism*; D007378:Interleukins / Q000378:metabolism*; D008297:Male; D000097043:Interleukin-22
chemical_list: D020381:Interleukin-17; D007378:Interleukins; D000097043:Interleukin-22; C508717:IL17A protein, human
keywords: EV71; HFMD; circulating Th17 cell; circulating Th22 cell