title: [Reactivated Expression of MicroRNA-124 in Patients with Myelodysplastic Syndromes after Demethylating Therapy].
abstract: OBJECTIVE
  To explore the role of microRNA-124(miR-124) in the pathogenesis of myelodysplastic syndromes(MDS) through detecting the expression level of miR-124 in bone marrow mononuclear cells(MNC) of MDS patients before and after demethylating therapy with decitabine.
  
  
  METHODS
  The expression levels of miR-124 in the MNC of 35 MDS patients and 10 healthy donors were detected with stem-loop quantitative real time polymerase chain reaction assay.
  
  
  RESULTS
  The expression level of miR-124 was lower in MDS patients than that in healthy donors. The difference was not statistically significant between patients with low-risk MDS subtypes (RA and RCMD) and control, but statistically significant between patients with high-risk MDS subtypes (RAEB1, RAEB2 and CMML) and control. This study also proved that expression of miR-124 was reactivated in 7 out of 18 MDS patients after treatment with low dose decitabine.
  
  
  CONCLUSIONS
  The hypermethylation and silencing of miR-124 may be an important factor in the clonal transformation of MDS cells.
authors.affiliation: Department of Hematology, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China. E-mail: mengys@hotmail.com.
authors.forename: Yue-Sheng
authors.initials: YS
authors.lastname: Meng
authors.affiliation: Department of Hematology, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Song
authors.initials: S
authors.lastname: Gao
authors.affiliation: Department of Hematology, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Fan-Li
authors.initials: FL
authors.lastname: Hua
authors.affiliation: Department of Hematology, Jinshan Hospital Affiliated to Fudan University, Shanghai 201508, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
mesh_terms: D000368:Aged; D001374:Azacitidine / Q000031:analogs & derivatives; D001854:Bone Marrow Cells; D002452:Cell Count; D019175:DNA Methylation; D000077209:Decitabine; D006801:Humans; D035683:MicroRNAs; D009190:Myelodysplastic Syndromes; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C509207:MIRN124 microRNA, human; D035683:MicroRNAs; D000077209:Decitabine; D001374:Azacitidine