title: Consolidated ethanol production from Jerusalem artichoke tubers at elevated temperature by Saccharomyces cerevisiae engineered with inulinase expression through cell surface display.
abstract: Ethanol fermentation from Jerusalem artichoke tubers was performed at elevated temperatures by the consolidated bioprocessing strategy using Saccharomyces cerevisiae MK01 expressing inulinase through cell surface display. No significant difference was observed in yeast growth when temperature was controlled at 38 and 40 °C, respectively, but inulinase activity with yeast cells was substantially enhanced at 40 °C. As a result, enzymatic hydrolysis of inulin was facilitated and ethanol production was improved with 89.3 g/L ethanol produced within 72 h from 198.2 g/L total inulin sugars consumed. Similar results were also observed in ethanol production from Jerusalem artichoke tubers with 85.2 g/L ethanol produced within 72 h from 185.7 g/L total sugars consumed. On the other hand, capital investment on cooling facilities and energy consumption for running the facilities would be saved, since regular cooling water instead of chill water could be used to cool down the fermentation system.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116023, China.
authors.forename: M Mahfuza
authors.initials: MM
authors.lastname: Khatun
authors.affiliation: School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
authors.forename: Chen-Guang
authors.initials: CG
authors.lastname: Liu
authors.affiliation: School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. xqzhao@sjtu.edu.cn.
authors.forename: Xin-Qing
authors.initials: XQ
authors.lastname: Zhao
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116023, China.
authors.forename: Wen-Jie
authors.initials: WJ
authors.lastname: Yuan
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian, 116023, China. fwbai@dlut.edu.cn.|School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China. fwbai@dlut.edu.cn.
authors.forename: Feng-Wu
authors.initials: FW
authors.lastname: Bai
mesh_terms: D000431:Ethanol / Q000378:metabolism*; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism; D006368:Helianthus / Q000737:chemistry*; D006868:Hydrolysis; D007218:Industrial Microbiology / Q000379:methods*; D007444:Inulin / Q000378:metabolism; D000074041:Microorganisms, Genetically-Modified / Q000235:genetics; D035281:Plant Tubers / Q000737:chemistry; D012441:Saccharomyces cerevisiae / Q000201:enzymology* / Q000235:genetics*; D013696:Temperature
chemical_list: D000431:Ethanol; D007444:Inulin; D006026:Glycoside Hydrolases; C023086:inulinase
keywords: Cell surface display; Consolidated bioprocessing; Ethanol production; Inulinase; Saccharomyces cerevisiae