title: Quercetin inhibits sodium nitrite-induced inflammation and apoptosis in different rats organs by suppressing Bax, HIF1-α, TGF-β, Smad-2, and AKT pathways.
abstract: The objective of this work is to study the protective effects of Quercetin against sodium nitrite-induced hypoxia on liver, lung, kidney and cardiac tissues, also to explore novel mechanism of this compound. Male albino rats were injected with sodium nitrite (75 mg/kg). Quercetin (200 mg kg-1,- i.p.) was administrated 24 and 1 h respectively prior to sodium nitrite intoxication, hypoxia significantly decreased hemoglobin concentration, while increased expressions of HIF, Bax, Smad-2, TGF-β, and AKT. However, administration of Quercetin played a modulatory role against the previous mentioned apoptotic factors protein expressions in all the studied tissues. On the other hand, Bcl-2 was downregulated by NaNO2, whereas concurrent treatment with Quercetin increased its expression. It was concluded that Quercetin possesses an anti-apoptotic action induced by NaNO2-intoxication via different mechanisms. Quercetin administration is recommended in areas of high altitudes to combat the hazard effect of hypoxia in different organs and in some diseases accompanied by hypoxic stress.
authors.affiliation: Pharmacology Department, Faculty of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Nouf Mohamed
authors.initials: NM
authors.lastname: Al-Rasheed
authors.affiliation: Pharmacology Department, Faculty of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Laila Mohamed
authors.initials: LM
authors.lastname: Fadda
authors.affiliation: Pharmacology Department, Faculty of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia.|Department of Biochemistry, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
authors.forename: Hala A
authors.initials: HA
authors.lastname: Attia
authors.affiliation: Department of Genetics and Cytology, National Research Center, Dokki, Egypt.|Preparatory Year Deanship, King Saud University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Hanaa Mahmoud
authors.initials: HM
authors.lastname: Ali
authors.affiliation: Pharmacology Department, Faculty of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia.|Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Nawal Mohamed
authors.initials: NM
authors.lastname: Al-Rasheed
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D051795:Hypoxia-Inducible Factor 1, alpha Subunit / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000188:drug therapy / Q000378:metabolism / Q000473:pathology; D008297:Male; D009928:Organ Specificity / Q000187:drug effects; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D011794:Quercetin / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D051899:Smad2 Protein / Q000378:metabolism*; D012977:Sodium Nitrite / Q000633:toxicity*; D016212:Transforming Growth Factor beta / Q000378:metabolism*; D051028:bcl-2-Associated X Protein / Q000378:metabolism*
chemical_list: C494828:Bax protein, rat; C497444:Hif1a protein, rat; D051795:Hypoxia-Inducible Factor 1, alpha Subunit; D051899:Smad2 Protein; C497784:Smad2 protein, rat; D016212:Transforming Growth Factor beta; D051028:bcl-2-Associated X Protein; D011794:Quercetin; D051057:Proto-Oncogene Proteins c-akt; D012977:Sodium Nitrite
keywords: AKT; Bax; HIF; Smad-2; TGF-β; hypoxia; quercetin