title: Neurogenesis-Promoting Natural Product α-Asarone Modulates Morphological Dynamics of Activated Microglia.
abstract: α-Asarone is an active constituent ofAcori Tatarinowii, one of the widely used traditional Chinese Medicine to treat cognitive defect, and recently is shown to promote neurogenesis. Here, we demonstrated that low level (3 μM) of α-asarone attenuated LPS-induced BV2 cell bipolar elongated morphological change, with no significant effect on the LPS-induced pro-inflammatory cytokine expressions. In addition, time-lapse analysis also revealed that α-asarone modulated LPS-induced BV2 morphological dynamics. Consistently a significant reduction in the LPS-induced Monocyte Chemoattractant Protein (MCP-1) mRNA and protein levels was also detected along with the morphological change. Mechanistic study showed that the attenuation effect to the LPS-resulted morphological modulation was also detected in the presence of MCP-1 antibodies or a CCR2antagonist. This result has also been confirmed in primary cultured microglia. Thein vivoinvestigation provided further evidence that α-asarone reduced the proportion of activated microglia, and reduced microglial tip number and maintained the velocity. Our study thus reveals α-asarone effectively modulates microglial morphological dynamics, and implies this effect of α-asarone may functionally relate to its influence on neurogenesis.
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesShanghai, China; Graduate School, University of Chinese Academy of Sciences, Chinese Academy of SciencesShanghai, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Cai
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesShanghai, China; Graduate School, University of Chinese Academy of Sciences, Chinese Academy of SciencesShanghai, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesShanghai, China; Graduate School, University of Chinese Academy of Sciences, Chinese Academy of SciencesShanghai, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Mao
authors.affiliation: State Key Laboratory of Cell Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of SciencesShanghai, China; School of Life Science and Technology, and the Collaborative Innovation Center for Brain Science, Tongji UniversityShanghai, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Pei
keywords: MCP-1; microglia; morphological dynamics; neuroinflammation; zebrafish; α-asarone