title: Targeted Metabolomic Analysis of Polyphenols with Antioxidant Activity in Sour Guava (Psidium friedrichsthalianum Nied.) Fruit.
abstract: Psidiumis a genus of tropical bushes belonging to the Myrtaceae family distributed in Central and South America. The polar extract ofPsidium friedrichsthalianumNied. was partitioned with ethyl ether, ethyl acetate, andn-butanol, and the total phenolic content and antioxidant activity were measured by Folin-Ciocalteu and ABTS assays, respectively. The ethyl acetate fraction exhibited both the highest phenolic content and antioxidant activity. Due to the complexity of this fraction, an analytical method for the comprehensive profiling of phenolic compounds was done by UPLC-ESI/QqQ in MRM (multiple reaction monitoring) mode. In this targeted analysis, 22 phenolic compounds were identified, among which several hydroxybenzoic, phenylacetic, and hydroxycinnamic acid derivatives were found. This is the first time that (+)-catechin, procyanidin B1, procyanidin B2, and (-)-epicatechin have been reported as constituents of sour guava. A fractionation by exclusion size, C18-column chromatography, and preparative RRLC (rapid resolution liquid chromatography) allowed us to confirm the presence of ellagic acid and isomeric procyanidins B, well-known bioactive compounds. The content of phenolic compounds in this fruit shows its potential for the development of functional foods.
authors.affiliation: Departamento de Química, Universidad Nacional de Colombia, AA 14490 Bogotá, Colombia. ctcuadrados@unal.edu.co.
authors.forename: Carmen Tatiana
authors.initials: CT
authors.lastname: Cuadrado-Silva
authors.affiliation: Instituto de Investigación en Ciencias de la Alimentación (CIAL) (CSIC-UAM), C/Nicolás Cabrera, 9, Campus de Cantoblanco, 28049 Madrid, Spain. m.delpozo@csic.es.
authors.forename: Maria Ángeles
authors.initials: MÁ
authors.lastname: Pozo-Bayón
authors.affiliation: Departamento de Química, Universidad Nacional de Colombia, AA 14490 Bogotá, Colombia. cosorior@unal.edu.co.
authors.forename: Coralia
authors.initials: C
authors.lastname: Osorio
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000085:Acetates / Q000737:chemistry; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification; D052160:Benzothiazoles / Q000037:antagonists & inhibitors; D044946:Biflavonoids / Q000737:chemistry* / Q000302:isolation & purification; D002392:Catechin / Q000737:chemistry* / Q000302:isolation & purification; D002850:Chromatography, Gel; D002851:Chromatography, High Pressure Liquid; D004610:Ellagic Acid / Q000737:chemistry / Q000302:isolation & purification; D004986:Ether / Q000737:chemistry; D059625:Liquid-Liquid Extraction / Q000379:methods; D055432:Metabolomics / Q000379:methods*; D010936:Plant Extracts / Q000737:chemistry; D059808:Polyphenols / Q000737:chemistry* / Q000302:isolation & purification; D044945:Proanthocyanidins / Q000737:chemistry* / Q000302:isolation & purification; D031647:Psidium / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry; D013451:Sulfonic Acids / Q000037:antagonists & inhibitors
chemical_list: D000085:Acetates; D000975:Antioxidants; D052160:Benzothiazoles; D044946:Biflavonoids; D010936:Plant Extracts; D059808:Polyphenols; D044945:Proanthocyanidins; D012997:Solvents; D013451:Sulfonic Acids; C479579:procyanidin B1; D004986:Ether; D004610:Ellagic Acid; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; C479580:procyanidin B2; C007650:ethyl acetate; D020001:1-Butanol; D002392:Catechin
keywords: ellagic acid; metabolomics; myrtaceae; targeted analysis; tropical fruit