title: HnRNP-L promotes prostate cancer progression by enhancing cell cycling and inhibiting apoptosis.
abstract: Expression of the RNA-binding protein HnRNP-L was previously shown to associate with tumorigenesis in liver and lung cancer. In this study, we examined the role of HnRNP-L in prostate cancer (Pca). We found that HnRNP-L is overexpressed in prostate tissue samples from 160 PC patients compared with tissue samples from 32 donors with cancers other than Pca. Moreover, HnRNP-L positively correlated with aggressive tumor characteristics. HnRNP-L knockdown inhibited cell proliferation and promoted cell apoptosis of Pca cell lines in vitro, and suppressed tumor growth when the cells were subcutaneously implanted in an athymic mouse model. Conversely, overexpression of HnRNP-L promoted cell proliferation and tumor growth while prohibiting cell apoptosis. HnRNP-L promoted cell proliferation and tumor growth in Pca in part by interacting with endogenous p53 mRNA, which was closely associated with cyclin p21. In addition, HnRNP-L affected cell apoptosis by directly binding the classical apoptosis protein BCL-2. These observations suggest HnRNP-L is an important regulatory factor that exerts pro-proliferation and anti-apoptosis effects in Pca through actions affecting the cell cycle and intrinsic apoptotic signaling. Thus HnRNP-L could potentially serve as a valuable molecular biomarker or therapeutic target in the treatment of Pca.
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Xumin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Department of Urology, Maternal and Health Care Hospital, Longgang District, Shenzhen 518036, P. R.China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Li
authors.affiliation: Department of Urology, Fourth Affiliated Hospital, Guangxi Medical University, Liuzhou 545005, P. R.China.
authors.forename: Jincan
authors.initials: J
authors.lastname: He
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Liren
authors.initials: L
authors.lastname: Zhong
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Fangpeng
authors.initials: F
authors.lastname: Shu
authors.affiliation: Department of Urology, Affiliated Weihai Second Municipal Hospital of Qingdao University, Weihai 264200, P. R.China.
authors.forename: Rongwei
authors.initials: R
authors.lastname: Xing
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Daojun
authors.initials: D
authors.lastname: Lv
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Bin
authors.initials: B
authors.lastname: Lei
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Bo
authors.initials: B
authors.lastname: Wan
authors.affiliation: Department of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, 518036, P. R.China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Huayan
authors.initials: H
authors.lastname: Wu
authors.affiliation: Department of Urology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.|Department of Urology, Peking University Shenzhen Hospital, Shenzhen PKU-HKUST Medical Center, Shenzhen, 518036, P. R.China.
authors.forename: Xiangming
authors.initials: X
authors.lastname: Mao
authors.affiliation: Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, P. R.China.
authors.forename: Yaguang
authors.initials: Y
authors.lastname: Zou
mesh_terms: D000818:Animals; D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D002453:Cell Cycle; D049109:Cell Proliferation; D018450:Disease Progression; D015972:Gene Expression Regulation, Neoplastic; D034664:Heterogeneous-Nuclear Ribonucleoprotein L / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D051379:Mice; D008819:Mice, Nude; D060787:Neoplasm Grading; D009367:Neoplasm Staging; D011379:Prognosis; D011471:Prostatic Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology*; D014407:Tumor Cells, Cultured; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D034664:Heterogeneous-Nuclear Ribonucleoprotein L
keywords: HnRNP-L; apoptosis; molecular mechanism; proliferation; prostate cancer