title: Lipid droplet dynamics duringSchizosaccharomyces pombesporulation and their role in spore survival.
abstract: Upon nitrogen starvation, the fission yeastSchizosaccharomyces pombeforms dormant spores; however, the mechanisms by which a spore sustains life without access to exogenous nutrients remain unclear. Lipid droplets are reservoirs of neutral lipids that act as important cellular energy resources. Using live-cell imaging analysis, we found that the lipid droplets of mother cells redistribute to their nascent spores. Notably, this process was actin polymerization-dependent and facilitated by the leading edge proteins of the forespore membrane. Spores lacking triacylglycerol synthesis, which is essential for lipid droplet formation, failed to germinate. Our results suggest that the lipid droplets are important for the sustenance of life in spores.
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.
authors.forename: Hui-Ju
authors.initials: HJ
authors.lastname: Yang
authors.affiliation: Advance ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Osakada
authors.affiliation: Advance ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.|Japan Women's University, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Kojidani
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Japan.|Advance ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Tokuko
authors.initials: T
authors.lastname: Haraguchi
authors.affiliation: Graduate School of Frontier Biosciences, Osaka University, Suita, Japan hiraoka@fbs.osaka-u.ac.jp.|Advance ICT Research Institute Kobe, National Institute of Information and Communications Technology, Kobe, Japan.
authors.forename: Yasushi
authors.identifier: http://orcid.org/0000-0001-9407-8228
authors.initials: Y
authors.lastname: Hiraoka
keywords: Actin; Forespore membrane; Germination; Lipid droplet; Septation initiation network; Spore