title: Structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed Monostroma angicava.
abstract: An anticoagulant-active polysaccharide PF2 was extracted with boiling water from the green seaweed Monostroma angicava, further purified by anion-exchange and size-exclusion chromatography. PF2 was a rhamnan-type sulfated polysaccharide with molecular weight of about 88.1kDa. Results of chemical and spectroscopic analyses demonstrated that PF2 consisted of→3)-α-l-Rhap-(1→ and →2)-α-l-Rhap-(1→residues, with partially branches at C-2 of→3)-α-l-Rhap-(1→residues. Sulfate groups were substituted at C-3 of →2)-α-l-Rhap-(1→ residues. The sulfated polysaccharide PF2 had a high anticoagulant action, and the mechanism of anticoagulant activity mediated by PF2 was mainly attributed to strong potentiation thrombin by heparin cofactor II. PF2 also exhibited weak effect on antithrombin-dependent thrombin or factor Xa inhibition. The fibrin(ogen)olytic activity and thrombolytic activity of PF2 were also evaluated. The investigation revealed that PF2 was a novel sulfated rhamnan differing from previously described sulfated polysaccharides from green seaweed and could be a potential anticoagulant polysaccharide.
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Department of Food and Biochemical Engineering, Yantai Vocational College, Yantai 264670, China.
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Xue
authors.initials: X
authors.lastname: Liu
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Xiaoxi
authors.initials: X
authors.lastname: He
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Shuyao
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Sujian
authors.initials: S
authors.lastname: Cao
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Xia
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Huali
authors.initials: H
authors.lastname: Xian
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Qin
authors.affiliation: Key Laboratory of Marine Drugs of Ministry of Education, Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China. Electronic address: wenjunmqd@hotmail.com.
authors.forename: Wenjun
authors.initials: W
authors.lastname: Mao
mesh_terms: D000818:Animals; D000925:Anticoagulants / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology; D001777:Blood Coagulation / Q000187:drug effects*; D006801:Humans; D011134:Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D012624:Seaweed / Q000737:chemistry*; D013431:Sulfates / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000925:Anticoagulants; D011134:Polysaccharides; D013431:Sulfates
keywords: Acetonitrile (PubChem CID: 6342); Anticoagulant activity; Barium rhodizonic acid (PubChem CID: 45048930); Dimethyl sulfoxide (PubChem CID: 679); Heparin (PubChem CID: 25244225); Inhibition mechanism; Iodomethane (PubChem CID: 6328); Monostroma angicava; Pullunan (PubChem CID: 92024139); Pyridine (PubChem CID: 1049); Rhamnose (PubChem CID: 25310); Structure; Sulfated polysaccharide; d-Glucuronic acid (PubChem CID: 94715); l-Phenyl-3-methyl-5-pyrazolone (PubChem CID: 90474051)