title: Efficacious fluorescence turn-on probe for high-contrast imaging of human cells overexpressing quinone reductase activity.
abstract: We report a new turn-on substrate probe whose intense fluorescent reporter signature is selectively provided upon probe activation by the cancer-associated oxidoreductase, hNQO1. The extremely high fluorescence turn-on of the probe was utilized to generate fluorescence microscope images of hNQO1-expressing, tumor-derived colorectal and ovarian cancer cells with unprecedented positive signal-to-negative background ratios (PNRs), a key step toward probe application in guided surgical removal of diseased tissues.
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803-1804, USA. tunnel@LSU.edu.
authors.forename: Quinn A
authors.initials: QA
authors.lastname: Best
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803-1804, USA. tunnel@LSU.edu.
authors.forename: Bijeta
authors.initials: B
authors.lastname: Prasai
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803-1804, USA. tunnel@LSU.edu.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rouillere
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803-1804, USA. tunnel@LSU.edu.
authors.forename: Amanda E
authors.initials: AE
authors.lastname: Johnson
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, Louisiana 70803-1804, USA. tunnel@LSU.edu.
authors.forename: Robin L
authors.identifier: http://orcid.org/0000-0002-4769-552X
authors.initials: RL
authors.lastname: McCarley
mesh_terms: D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry*; D006801:Humans; D016660:NAD(P)H Dehydrogenase (Quinone) / Q000096:biosynthesis* / Q000378:metabolism*; D061848:Optical Imaging
chemical_list: D005456:Fluorescent Dyes; D016660:NAD(P)H Dehydrogenase (Quinone); C447479:NQO1 protein, human