title: HPLC-ELSD Quantification and Centrifugal Partition Chromatography Isolation of 8-O-Acetylharpagide from Oxera coronata (Lamiaceae).
abstract: BACKGROUND
  Iridoid glycosides possess highly functionalised monoterpenoid aglycon with several contiguous stereocentres. For the most common, they are often present in quantities reaching several percentage of the fresh plant weight, and thus they may be regarded as starting material for the synthesis of a number of new chiral and bioactive molecules.
  
  
  OBJECTIVE
  To quantify and to isolate 8-O-acetylharpagide (AH) from several extracts of Oxera coronata R.P.J. de Kok, a Lamiaceae species endemic to New Caledonia, using HPLC-ELSD (evaporative light scattering detector) and centrifugal partition chromatography (CPC).
  
  
  METHODS
  Oxera coronata produces high amounts of AH in leaves, twigs and fruits. Water and methanol extracts of these plant parts were prepared. The content of AH in each extract was quantified by HPLC-ELSD, using acetonitrile-water (+0.1% formic acid) gradient elution. The HPLC method was validated for precision, linearity, limit of detection (LOD), limit of quantification (LOQ) and accuracy. A ternary solvent system ethyl acetate/n-propanol/water (3:2:5, v/v/v) was selected and applied to recover the target compound using Spot CPC from the leaves aqueous extract.
  
  
  RESULTS
  HPLC-ELSD analysis followed by CPC purification led to the efficient isolation of AH from O. coronata leaves aqueous extract.
  
  
  CONCLUSIONS
  HPLC-ELSD has proven to be a well-adapted detection and quantification method for iridoid glycosides, while CPC confirmed to be an efficient technique for the isolation of polar compounds. Copyright © 2016 John Wiley & Sons, Ltd.
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS-ICSN, UPR2301, Université Paris-Saclay, 1, avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Remeur
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS-ICSN, UPR2301, Université Paris-Saclay, 1, avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
authors.forename: Erell
authors.initials: E
authors.lastname: Le Borgne
authors.affiliation: Université Paris Descartes, Faculté de Pharmacie de Paris, UMR CNRS 8638, Laboratoire de Pharmacognosie, 4 avenue de l'Observatoire, 75006, Paris, France.
authors.forename: Léa
authors.initials: L
authors.lastname: Gauthier
authors.affiliation: Université Paris Descartes, Faculté de Pharmacie de Paris, UMR CNRS 8638, Laboratoire de Pharmacognosie, 4 avenue de l'Observatoire, 75006, Paris, France.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Grougnet
authors.affiliation: Université Paris Descartes, Faculté de Pharmacie de Paris, UMR CNRS 8638, Laboratoire de Pharmacognosie, 4 avenue de l'Observatoire, 75006, Paris, France.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Deguin
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS-ICSN, UPR2301, Université Paris-Saclay, 1, avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Poullain
authors.affiliation: Institut de Chimie des Substances Naturelles, CNRS-ICSN, UPR2301, Université Paris-Saclay, 1, avenue de la Terrasse, 91198, Gif-sur-Yvette, France.
authors.forename: Marc
authors.initials: M
authors.lastname: Litaudon
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D002853:Chromatography, Liquid / Q000295:instrumentation / Q000379:methods; D005638:Fruit / Q000737:chemistry; D019686:Lamiaceae / Q000737:chemistry*; D009510:New Caledonia; D010936:Plant Extracts / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry; D011714:Pyrans / Q000032:analysis / Q000302:isolation & purification*
chemical_list: D010936:Plant Extracts; D011714:Pyrans; C077626:8-O-acetylharpagide
keywords: HPLC-ELSD; Lamiaceae; Oxera coronata; centrifugal partition chromatography; iridoid glycoside