title: MicroRNA-134-3p is a novel potential inhibitor of human ovarian cancer stem cells by targeting RAB27A.
abstract: The cluster of microRNAs (miRNAs) in the DLK1-DIO3 genomic imprinted region contains several miRNAs that have a significant regulatory role in tumor proliferation and invasion. One of these miRNAs is miR-134-3p, and its expression changes significantly in human ovarian cancer stem cells (OCSCs) and in CD44-/CD133- ovarian cancer. The results of a luciferase assay showed that miR-134-3p silenced RAB27A by binding to the 3'-UTR of RAB27A mRNA. Overexpression of miR-134-3p in human OCSCs can not only inhibit the expression of RAB27A but also can effectively downregulate the expression of some tumor proliferation and invasion genes. Overexpression of miR-134-3p can not only inhibit the in vitro proliferation and cell cycle progression of human OCSCs but also can decrease the tumorigenicity in nude mice.
authors.affiliation: Department of Pharmaceutical Science, Shenzhen People's Hospital, Second Clinical College, Jinan University, Shenzhen 518020, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Chang
authors.affiliation: Department of Pathology, Yale University School of Medicine, New Haven 06520, USA; Shanghai Tenth People's Hospital, Medical School, Tongji University, Shanghai 200072, China. Electronic address: te.liu@yale.edu.
authors.forename: Te
authors.initials: T
authors.lastname: Liu
authors.affiliation: Laboratoire PROTEE, Bâtiment R, Université du Sud Toulon-Var, 83957 La Garde Cedex, France.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Department of Medical Oncology, Shanghai Changzheng Hospital, Affiliated to The Second Military Medical University, Shanghai 200070, China.
authors.forename: Wenxing
authors.initials: W
authors.lastname: Qin
authors.affiliation: Department of Pharmaceutical Science, Shenzhen People's Hospital, Second Clinical College, Jinan University, Shenzhen 518020, China. Electronic address: yht2113@126.com.
authors.forename: Hongtu
authors.initials: H
authors.lastname: Yang
authors.affiliation: Gongli Hospital Affiliated to the Second Military Medicical University in Pudong New Area of Shanghai City, Shanghai 200135, China. Electronic address: 406526258@qq.com.
authors.forename: Juan
authors.initials: J
authors.lastname: Chen
mesh_terms: D018262:Adenocarcinoma, Clear Cell / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D000328:Adult; D000368:Aged; D000818:Animals; D001483:Base Sequence; D001665:Binding Sites; D049109:Cell Proliferation; D005260:Female; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D017930:Genes, Reporter; D006801:Humans; D008156:Luciferases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D009368:Neoplasm Transplantation; D014411:Neoplastic Stem Cells / Q000378:metabolism* / Q000473:pathology; D010051:Ovarian Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D061251:Primary Cell Culture; D015398:Signal Transduction; D020691:rab GTP-Binding Proteins / Q000235:genetics* / Q000378:metabolism; D000075745:rab27 GTP-Binding Proteins
chemical_list: C569273:MIRN134 microRNA, human; D035683:MicroRNAs; D000075745:rab27 GTP-Binding Proteins; D008156:Luciferases; C066694:RAB27A protein, human; D020691:rab GTP-Binding Proteins
keywords: DLK1-DIO3 microRNA cluster; MicroRNA-134-3p (miR-134-3p); Ovarian cancer stem cells (OCSCs); RAB27A