title: Comparative phenotypic transcriptional characterization of human full-term placenta-derived mesenchymal stromal cells compared to bone marrow-derived mesenchymal stromal cells after differentiation in myogenic medium.
abstract: Placenta-derived mesenchymal stromal cells (pMSCs) are a very attractive source of MSCs. In this short report we evaluated the expression of phenotypic markers from fetal and maternal pMSCs after exposure to myogenic medium commonly used to differentiate bone marrow MSCs (bmMSCs) to smooth muscle-like cells (SMCs). In order to reveal differences between these different MSC sources, cells were expanded and differentiated to elucidate whether this differentiation protocol facilitated efficient differentiation of SMCs from human pMSCs. We report that TGF-β1, PDGF and ascorbic acid is not sufficient to produce SMCs from pMSCs.
authors.affiliation: University of Freiburg, Department of Orthopedics and Trauma Surgery, Germany; Clinical Research Group KFO 273, Department of Urology, University of Tübingen, Germany. Electronic address: melaniehar@gmail.com.
authors.forename: Melanie L
authors.initials: ML
authors.lastname: Hart
authors.affiliation: Clinical Research Group KFO 273, Department of Urology, University of Tübingen, Germany.
authors.forename: Marvin
authors.initials: M
authors.lastname: Kaupp
authors.affiliation: Clinical Research Group KFO 273, Department of Urology, University of Tübingen, Germany.
authors.forename: Juliane
authors.initials: J
authors.lastname: Brun
authors.affiliation: Clinical Research Group KFO 273, Department of Urology, University of Tübingen, Germany.
authors.forename: Wilhelm K
authors.initials: WK
authors.lastname: Aicher
mesh_terms: D001854:Bone Marrow Cells / Q000166:cytology / Q000378:metabolism*; D002454:Cell Differentiation / Q000502:physiology*; D003470:Culture Media; D005260:Female; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism*; D024510:Muscle Development / Q000502:physiology*; D010920:Placenta / Q000166:cytology / Q000378:metabolism*; D011247:Pregnancy; D014158:Transcription, Genetic
chemical_list: D003470:Culture Media
keywords: Bone marrow; Fetal; Maternal; Mesenchymal stromal cells; Myogenic differentiation; Placenta; Smooth muscle