title: [The role of heat shock proteins 27 and 70 in redox-dependent regulation of apoptosis in Jurkat tumor cells].
abstract: Heat shock proteins Hsp) act as molecular chaperones, protecting enzymes and other proteins against reactive oxygen species. The objective of the study was to investigate the role of Hsp27 in maintaining the balance of the glutathione system and Hsp70 concentrations as well as in implementing Jurkat tumor cell apoptosis. Addition of the Hsp27 inhibitor KRIBB3 (5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)-isoxazol) to Jurkat cells resulted in glutathione redox imbalance (increased GSSG and increased glutathione reductase activity), a decrease in Hsp70 concentrations, and also increased cell apoptosis as compared with to the intact cell culture. The proposed selective regulation of chaperone activity is a promising direction in regulating apoptosis at the cellular level.
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: O L
authors.initials: OL
authors.lastname: Nosareva
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Ryazantseva
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Stepovaya
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: E V
authors.initials: EV
authors.lastname: Shakhristova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Stepanova
authors.affiliation: Siberian State Medical University, Tomsk, Russia.
authors.forename: V S
authors.initials: VS
authors.lastname: Gulaya
mesh_terms: D000840:Anisoles / Q000494:pharmacology; D017209:Apoptosis; D005978:Glutathione / Q000378:metabolism*; D055551:HSP27 Heat-Shock Proteins / Q000037:antagonists & inhibitors / Q000378:metabolism*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism*; D006360:Heat-Shock Proteins; D006801:Humans; D007555:Isoxazoles / Q000494:pharmacology; D019169:Jurkat Cells; D018832:Molecular Chaperones; D010084:Oxidation-Reduction
chemical_list: C507623:5-(5-ethyl-2-hydroxy-4-methoxyphenyl)-4-(4-methoxyphenyl)isoxazole; D000840:Anisoles; D055551:HSP27 Heat-Shock Proteins; D018840:HSP70 Heat-Shock Proteins; C057725:HSPB1 protein, human; D006360:Heat-Shock Proteins; D007555:Isoxazoles; D018832:Molecular Chaperones; D005978:Glutathione
keywords: Jurkat tumor cells; apoptosis; cell redox status; glutathione system; heat shock proteins 27 and 70