title: The mechanism of a chitosan-collagen composite film used as biomaterial support for MC3T3-E1 cell differentiation.
abstract: Natural composite biomaterials are good structural supports for bone cells to regenerate lost bone. Here, we report that a chitosan-collagen composite film accelerated osteoblast proliferation, differentiation and matrix mineralization in MC3T3-E1 cells. Intriguingly, we observed that the film enhanced the phosphorylation of Erk1/2. We showed that the chitosan-collagen composite film increased the transcriptional activity of Runx2, which is an important factor regulating osteoblast differentiation downstream of phosphorylated Erk1/2. Consistent with this observation, we found that the chitosan-collagen composite film increased the expression of osteoblastic marker genes, including Type I Collagen and Runx2 in MC3T3-E1 cells. We conclude that this film promoted osteoblast differentiation and matrix mineralization through an Erk1/2-activated Runx2 pathway. Our findings provide new evidence that chitosan-collagen composites are promising biomaterials for bone tissue engineering in bone defect-related diseases.
authors.affiliation: Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha, Hunan, 410073, PR China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha, Hunan, 410073, PR China.
authors.forename: Gan
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha, Hunan, 410073, PR China.
authors.forename: Long
authors.initials: L
authors.lastname: Liu
authors.affiliation: Department of Chemistry and Biology, College of Science, National University of Defense Technology, Changsha, Hunan, 410073, PR China.
authors.forename: Dongyi
authors.initials: D
authors.lastname: Zhang
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000378:metabolism*; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D002460:Cell Line; D048271:Chitosan / Q000378:metabolism*; D003094:Collagen / Q000378:metabolism*; D050659:Core Binding Factor Alpha 1 Subunit / Q000378:metabolism; D020869:Gene Expression Profiling; D020935:MAP Kinase Signaling System; D051379:Mice; D010006:Osteoblasts / Q000502:physiology*; D010766:Phosphorylation; D011499:Protein Processing, Post-Translational; D015398:Signal Transduction
chemical_list: D001672:Biocompatible Materials; D050659:Core Binding Factor Alpha 1 Subunit; D003094:Collagen; D048271:Chitosan