title: UV-B radiation increases anthocyanin levels in cotyledons and inhibits the growth of common buckwheat seedlings.
abstract: The impact of short-term UV-B treatment on the content of individual flavonoids and photosynthetic pigments in cotyledons and the growth of common buckwheat (Fagopyrum esculentum Moench) seedlings was investigated. Seeds of four common buckwheat cultivars were germinated in darkness over a period of 4 days and acclimatized for 2 days under a 16/8 h light/dark photoperiod at 24/18 °C day/night, and exposure to 100-120 μmol ∙ m-2∙ s-1of photosynthetically active radiation (PAR). Seedlings were divided into three batches, including two batches subjected to different doses of UV-B (5 W ∙ m-2and 10 W ∙ m-2, one hour per day) for 5 days, and a control group exposed to PAR only. Exposure to UV-B increased anthocyanin levels in the cotyledons of all examined cultivars, it inhibited hypocotyl elongation, but did not affect the content of photosynthetic pigments. Flavone concentrations increased in cv. Red Corolla and Kora, remained constant in cv. Panda and decreased in cv. Hruszowska. Exposure to UV-B decreased rutin levels in cv. Hruszowska, but not in the remaining cultivars. Cultivars Hruszowska, Panda and Kora appeared to be less resistant to UV-B than Red Corolla. Higher resistance to UV-B radiation in Red Corolla can probably be attributed to its higher content of anthocyanins and rutin in comparison with the remaining cultivars.
authors.affiliation: Department of Plant Physiology and Genetics, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities , 08-110 Siedlce, Prusa 12 , Poland.
authors.forename: Henryk
authors.initials: H
authors.lastname: Dębski
authors.affiliation: Department of Plant Physiology and Genetics, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities , 08-110 Siedlce, Prusa 12 , Poland.
authors.forename: Magdalena
authors.initials: M
authors.lastname: Szwed
authors.affiliation: Department of Food Chemistry and Biodynamics, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences , Tuwima 10, 10-747 Olsztyn , Poland.
authors.forename: WiesŁaw
authors.initials: W
authors.lastname: Wiczkowski
authors.affiliation: Department of Food Chemistry and Biodynamics, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences , Tuwima 10, 10-747 Olsztyn , Poland.
authors.forename: Dorota
authors.initials: D
authors.lastname: Szawara-Nowak
authors.affiliation: Department of Food Chemistry and Biodynamics, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences , Tuwima 10, 10-747 Olsztyn , Poland.
authors.forename: Natalia
authors.initials: N
authors.lastname: Bączek
authors.affiliation: Department of Plant Physiology and Genetics, Faculty of Natural Sciences, Siedlce University of Natural Sciences and Humanities , 08-110 Siedlce, Prusa 12 , Poland.
authors.forename: Marcin
authors.initials: M
authors.lastname: Horbowicz
mesh_terms: D000872:Anthocyanins / Q000378:metabolism / Q000528:radiation effects*; D002338:Carotenoids / Q000378:metabolism / Q000528:radiation effects*; D002734:Chlorophyll / Q000378:metabolism / Q000528:radiation effects; D018548:Cotyledon / Q000378:metabolism / Q000528:radiation effects*; D019612:Fagopyrum / Q000254:growth & development / Q000378:metabolism / Q000528:radiation effects*; D047309:Flavones / Q000378:metabolism / Q000528:radiation effects*; D005419:Flavonoids / Q000378:metabolism / Q000528:radiation effects; D044948:Flavonols / Q000378:metabolism / Q000528:radiation effects*; D018546:Hypocotyl / Q000254:growth & development / Q000528:radiation effects; D010636:Phenols / Q000378:metabolism / Q000528:radiation effects; D012431:Rutin / Q000378:metabolism / Q000528:radiation effects; D036226:Seedlings / Q000254:growth & development / Q000528:radiation effects*; D014466:Ultraviolet Rays
chemical_list: D000872:Anthocyanins; D047309:Flavones; D005419:Flavonoids; D044948:Flavonols; D010636:Phenols; D002734:Chlorophyll; D002338:Carotenoids; D012431:Rutin
keywords: UV-B radiation; chlorophyll; common buckwheat; flavonoid; seedling growth