title: Chemical Composition and Biological Prospects of Essential Oils and Extracts of Aphyllocladus spartioides Growing in Northwest Argentina.
abstract: Aphyllocladus spartioides Wedd. is a native and aromatic herb used in traditional medicine, however it is still poorly investigated. In this work, the volatile profile of A. spartioides growing in Hornillos-Northwest Argentina was determined by GC/MS/FID and the phenolic compounds of hydroethanolic and decoction extracts were analyzed by HPLC-DAD. The antibacterial potential, antioxidant activity and α-glucosidase inhibition activity were checked by in vitro assays. The volatile profile allowed the identification of 68 compounds, being α-pinene and cadinene the main ones. Eighteen phenolics were identified, isorhamnetin derivatives and different phenolic acid derivatives were found in higher amounts, mainly in the hydroethanolic extract. A concentration-dependent activity was noticed against DPPH·, O2·-, ·NO and α-glucosidase, these activities being reported for the first time. Hydroethanolic extract was most active against DPPH·,·NO and α-glucosidase (IC50= 79, 206 and 181 μg/ml). Decoction extract proved to be better against O2·- (IC50= 20 μg/ml). Regarding the antibacterial activity, hydroethanolic extract was more active compared with decoction and essential oil. MICs of 0.3 - 0.6 mg/ml were obtained against Staphylococcus aureus, Bacillus cereus, and Micrococcus luteus. Results suggest that the extracts of A. spartioides from Northwest Argentina may be interesting to use as a source of natural antioxidants/antimicrobials for pharmaceutical incorporations or food supplementation.
authors.affiliation: PRONOA/Laboratorio de Productos Naturales del NOA, CIT-Jujuy-Universidad Nacional de Jujuy-CONICET, Ítalo Palanca 10, CP4600, S. S. de Jujuy, Argentina.|REQUIMTE/Laboratório de Farmacognosia, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, 228 Rua de Jorge Viterbo Ferreira, 4050-313, Porto, Portugal.
authors.forename: Liliana
authors.initials: L
authors.lastname: Celaya
authors.affiliation: PRONOA/Laboratorio de Productos Naturales del NOA, CIT-Jujuy-Universidad Nacional de Jujuy-CONICET, Ítalo Palanca 10, CP4600, S. S. de Jujuy, Argentina.
authors.forename: Carmen
authors.initials: C
authors.lastname: Viturro
authors.affiliation: LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, 4200-465, Porto, Portugal.|CICS - UBI - Health Sciences Research Centre, University of Beira Interior, 6201-506, Covilhã, Portugal.
authors.forename: Luís R
authors.initials: LR
authors.lastname: Silva
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry; D000890:Anti-Infective Agents / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry*; D001118:Argentina; D019659:Asteraceae / Q000737:chemistry*; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes / Q000302:isolation & purification; D009822:Oils, Volatile / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D000890:Anti-Infective Agents; D000975:Antioxidants; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; C005451:alpha-pinene
keywords: 
  Aphyllocladus spartioides
  ; Bioactive compounds; Essential oils; Polar extracts