title: Microfluidic Reactors Based on Rechargeable Catalytic Porous Supports: Heterogeneous Enzymatic Catalysis via Reversible Host-Guest Interactions.
abstract: We report on the fabrication of a microfluidic device in which the reservoir contains a porous surface with enzymatic catalytic activity provided by the reversible immobilization of horseradish peroxidase onto micrometer size pores. The porous functional reservoir was obtained by the Breath Figures approach by casting in a moist environment a solution containing a mixture of high molecular weight polystyrene (HPS) and a poly(styrene-co-cyclodextrin based styrene) (P(S-co-SCD)) statistical copolymer. The pores enriched in CD were employed to immobilize horseradish peroxidase (previously modified with adamantane) by host-guest interactions (HRP-Ada). These surfaces exhibit catalytic activity that remains stable during several reaction cycles. Moreover, the porous platforms could be recovered by using free water-soluble β-CD with detergents. An excess of β-CD/TritonX100 in solution disrupts the interactions between HRP-Ada and the CD-modified substrate thus allowing us to recover the employed enzyme and reuse the platform.
authors.affiliation: Mechano(Bio)Chemistry, Max Planck Institute of Colloids and Interfaces , Science Park Potsdam-Golm, 14424 Potsdam, Germany.
authors.forename: Alberto Sanz
authors.initials: AS
authors.lastname: de León
authors.forename: Nelson
authors.initials: N
authors.lastname: Vargas-Alfredo
authors.forename: Alberto
authors.initials: A
authors.lastname: Gallardo
authors.forename: Alfonso
authors.initials: A
authors.lastname: Fernández-Mayoralas
authors.forename: Agatha
authors.initials: A
authors.lastname: Bastida
authors.affiliation: Departamento de Química-Física Aplicada, Facultad de Ciencias, Universidad Autónoma de Madrid , C/Francisco Tomás y Valiente 7, Cantoblanco, 28049 Madrid, Spain.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Muñoz-Bonilla
authors.forename: Juan
authors.identifier: http://orcid.org/0000-0003-2464-1040
authors.initials: J
authors.lastname: Rodríguez-Hernández
keywords: breath figures; enzymatic catalysis; heterogeneous catalysis; host−guest interactions; reusable bioreactors; surface modification