title: Oxidation of Iron under Physiologically Relevant Conditions in Biological Fluids from Healthy and Alzheimer's Disease Subjects.
abstract: Ferroxidase activity has been reported to be altered in various biological fluids in neurodegenerative disease, but the sources contributing to the altered activity are uncertain. Here we assay fractions of serum and cerebrospinal fluid with a newly validated triplex ferroxidase assay. Our data indicate that while ceruloplasmin, a multicopper ferroxidase, is the predominant source of serum activity, activity in CSF predominantly derives from a <10 kDa component, specifically from polyanions such as citrate and phosphate. We confirm that in human biological samples, ceruloplasmin activity in serum is decreased in Alzheimer's disease, but in CSF a reduction of activity in Alzheimer's disease originates from the polyanion component.
authors.affiliation: Oxidation Biology Unit, The Florey Institute of Neuroscience and Mental Health, The University of Melbourne , Parkville, Victoria 3010, Australia.|Neuropharmacology Laboratory, Department of Pharmacology and Therapeutics, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Linh Q
authors.initials: LQ
authors.lastname: Lam
authors.affiliation: Oxidation Biology Unit, The Florey Institute of Neuroscience and Mental Health, The University of Melbourne , Parkville, Victoria 3010, Australia.|School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds , Leeds LS2 9JT, West Yorkshire, United Kingdom.
authors.forename: Bruce X
authors.initials: BX
authors.lastname: Wong
authors.affiliation: Neuropharmacology Laboratory, Department of Pharmacology and Therapeutics, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Tony
authors.initials: T
authors.lastname: Frugier
authors.affiliation: Oxidation Biology Unit, The Florey Institute of Neuroscience and Mental Health, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Qiao-Xin
authors.initials: QX
authors.lastname: Li
authors.affiliation: Department of Medicine, Royal Melbourne Hospital, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Steven J
authors.initials: SJ
authors.lastname: Collins
authors.affiliation: Oxidation Biology Unit, The Florey Institute of Neuroscience and Mental Health, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Ashley I
authors.initials: AI
authors.lastname: Bush
authors.affiliation: Neuropharmacology Laboratory, Department of Pharmacology and Therapeutics, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Peter J
authors.initials: PJ
authors.lastname: Crack
authors.affiliation: Oxidation Biology Unit, The Florey Institute of Neuroscience and Mental Health, The University of Melbourne , Parkville, Victoria 3010, Australia.|School of Biomedical Sciences, Faculty of Biological Sciences, University of Leeds , Leeds LS2 9JT, West Yorkshire, United Kingdom.
authors.forename: James A
authors.identifier: 0000-0002-8762-2245
authors.initials: JA
authors.lastname: Duce
mesh_terms: D000544:Alzheimer Disease / Q000378:metabolism*; D002555:Cerebrospinal Fluid / Q000201:enzymology*; D002570:Ceruloplasmin / Q000032:analysis / Q000378:metabolism*; D006801:Humans; D007501:Iron / Q000378:metabolism*; D010084:Oxidation-Reduction; D044967:Serum / Q000201:enzymology*
chemical_list: D007501:Iron; D002570:Ceruloplasmin
keywords: Ferroxidase; ceruloplasmin; iron; neurodegenerative disease; oxidation; polyanion