title: Bioinformatics insight into glycosyltransferase gene expression in gastric cancer: POFUT1 is a potential biomarker.
abstract: Aberrant glycosylation plays an important role in the progression of gastric cancer. Although many enzymes are involved in glycoconjugate synthesis, little information is known about glycosyltransferase (GT)'s alteration in gastric cancer. To give an insight into the differential expression of GTs as obtained from the TCGA database, bioinformatics analysis of 415 gastric cancer samples and 35 normal samples was performed. Using a 0.6-fold change threshold to compare GT expression in normal and cancer tissues, it was noted that 36 GTs were upregulated including POFUT1 and 30 GTs were downregulated in cancer tissues (P < 0.01). Enriched pathway analysis indicated that POFUT1 was correlated positively with cell cycle and cell carcinoma process but correlated negatively with cell adhesion and apoptosis (P < 0.05). Furthermore, immunohistochemistry analysis verified that 33 out of 51 tumor tissues had detectable POFUT1 expression. POFUT1-positive samples were associated with aggressive tumor phenotypes like higher T and N classification (P = 0.026, 0.042) as well as poor differentiation (P = 0.031). No correlation was observed between POFUT1 expression and age, gender, stage, and Lauren classification. These results suggested that POFUT1 is increased and may serve as a potential biomarker in gastric cancer.
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Tongji University, 389Xincun Road, Putuo District, Shanghai, 200065, PR China. Electronic address: 2011dongshuang@tongji.edu.cn.
authors.forename: Shuang
authors.initials: S
authors.lastname: Dong
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Tongji University, 389Xincun Road, Putuo District, Shanghai, 200065, PR China. Electronic address: luckypinenut@126.com.
authors.forename: Zhe
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Tongji University, 389Xincun Road, Putuo District, Shanghai, 200065, PR China. Electronic address: bhuang83@126.com.
authors.forename: Binbin
authors.initials: B
authors.lastname: Huang
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Tongji University, 389Xincun Road, Putuo District, Shanghai, 200065, PR China. Electronic address: junjie81@126.com.
authors.forename: Junjie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Tongji University, 389Xincun Road, Putuo District, Shanghai, 200065, PR China. Electronic address: yanli_ge@126.com.
authors.forename: Yanli
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Tongji University, 389Xincun Road, Putuo District, Shanghai, 200065, PR China. Electronic address: 1192613646@qq.com.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Fan
authors.affiliation: Department of Gastroenterology, Tongji Hospital Affiliated to Tongji University, 389Xincun Road, Putuo District, Shanghai, 200065, PR China. Electronic address: wangzr929@126.com.
authors.forename: Zhirong
authors.initials: Z
authors.lastname: Wang
mesh_terms: D036002:ADP Ribose Transferases / Q000235:genetics / Q000378:metabolism; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics; D019295:Computational Biology / Q000379:methods; D005260:Female; D005647:Fucosyltransferases / Q000235:genetics* / Q000378:metabolism; D015971:Gene Expression Regulation, Enzymologic; D015972:Gene Expression Regulation, Neoplastic; D016695:Glycosyltransferases / Q000235:genetics* / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D008297:Male; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D008875:Middle Aged; D017350:N-Acetylgalactosaminyltransferases / Q000235:genetics / Q000378:metabolism; D011379:Prognosis; D012799:Sialyltransferases / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics* / Q000401:mortality* / Q000473:pathology; D016019:Survival Analysis
chemical_list: D014408:Biomarkers, Tumor; D008565:Membrane Proteins; D016695:Glycosyltransferases; D005647:Fucosyltransferases; C587353:GALNTL5 protein, human; D017350:N-Acetylgalactosaminyltransferases; C105148:polypeptide fucosyltransferase; D036002:ADP Ribose Transferases; C105234:ART4 protein, human; C046778:CMP-N-acetylneuraminate-poly-alpha-2,8-sialosyl sialyltransferase; D012799:Sialyltransferases
keywords: Bioinformatics; Gastric cancer; Glycosyltransferase; Immunohistochemistry; Protein O-fucosyltransferase 1