title: Precise integrin-targeting near-infrared imaging-guided surgical method increases surgical qualification of peritoneal carcinomatosis from gastric cancer in mice.
abstract: Peritoneal carcinomatosis from gastric cancer represents a common recurrent gastric cancer that seriously affects the survival, prognosis, and quality of life of patients at its advanced stage. In recent years, complete cytoreduction surgery in combination with hyperthermic intraperitoneal chemotherapy has been demonstrated to improve the survival and prognosis of patients with malignant tumors including peritoneal carcinomatosis from gastric cancer. Establishing viable methods of accurately assessing the tumor burden in patients with peritoneal carcinoma and correctly selecting suitable patients in order to improve cytoreduction surgical outcomes and reduce the risk of postoperative complications has become a challenge in the field of peritoneal carcinoma research. Here, we investigated peritoneal carcinomatosis from gastric cancer in a mouse model by using our self-developed surgical navigation system that combines optical molecular imaging with an integrin-targeting Arg-Gly-Asp-indocyanine green (RGD-ICG) molecular probe. The results showed that our diagnostic method could achieve a sensitivity and specificity of up to 93.93% and 100%, respectively, with a diagnostic index (DI) of 193.93% and diagnostic accuracy rate of 93.93%.Furthermore, the minimum tumor diameter measured during the surgery was 1.8 mm and the operative time was shortened by 3.26-fold when compared with the conventionally-treated control group. Therefore, our surgical navigation system that combines optical molecular imaging with an RGD-ICG molecular probe, could improve the diagnostic accuracy rate for peritoneal carcinomatosis from gastric cancer, shorten the operative time, and improve the quality of the cytoreduction surgery for peritoneal carcinomatosis from gastric cancer, thus providing a solid foundation for its future clinical development and application.
authors.affiliation: Department of General Surgery, The Chinese PLA General Hospital, Beijing, 100853, China.|Department of General Surgery, The First Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010059, China.|Key Laboratory of Molecular Imaging of the Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Haidong
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Key Laboratory of Molecular Imaging of the Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Chongwei
authors.initials: C
authors.lastname: Chi
authors.affiliation: Key Laboratory of Molecular Imaging of the Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Shang
authors.affiliation: Department of Basic Medical Science, Inner Mongolia Medical University, Hohhot, 010059, China.
authors.forename: Sha
authors.initials: S
authors.lastname: Rengaowa
authors.affiliation: Department of General Surgery, The Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Jianxin
authors.initials: J
authors.lastname: Cui
authors.affiliation: Key Laboratory of Molecular Imaging of the Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Jinzuo
authors.initials: J
authors.lastname: Ye
authors.affiliation: Key Laboratory of Molecular Imaging of the Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Shixin
authors.initials: S
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Molecular Imaging of the Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Yamin
authors.initials: Y
authors.lastname: Mao
authors.affiliation: Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou, 510280, China.
authors.forename: Caoting
authors.initials: C
authors.lastname: Zeng
authors.affiliation: Department of Ultrasound, General Hospital of the People's Liberation Army, Beijing, 100853, China.
authors.forename: Huiping
authors.initials: H
authors.lastname: Huo
authors.affiliation: Department of General Surgery, The Chinese PLA General Hospital, Beijing, 100853, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Chen
authors.affiliation: Key Laboratory of Molecular Imaging of the Chinese Academy of Sciences, Institute of Automation, Chinese Academy of Sciences, Beijing, 100190, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Tian
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor / Q000378:metabolism*; D065426:Cytoreduction Surgical Procedures / Q000379:methods*; D005260:Female; D005456:Fluorescent Dyes / Q000008:administration & dosage / Q000378:metabolism; D006801:Humans; D007208:Indocyanine Green / Q000008:administration & dosage / Q000378:metabolism; D039302:Integrin alphaVbeta3 / Q000378:metabolism*; D008163:Luminescent Measurements; D061986:MCF-7 Cells; D059146:Metastasectomy / Q000379:methods*; D008807:Mice, Inbred BALB C; D008819:Mice, Nude; D057054:Molecular Imaging / Q000379:methods*; D009842:Oligopeptides / Q000008:administration & dosage / Q000378:metabolism; D010534:Peritoneal Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000556:secondary / Q000601:surgery*; D011237:Predictive Value of Tests; D019265:Spectroscopy, Near-Infrared; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology; D025321:Surgery, Computer-Assisted / Q000379:methods*; D013997:Time Factors; D023041:Xenograft Model Antitumor Assays
chemical_list: D014408:Biomarkers, Tumor; D005456:Fluorescent Dyes; D039302:Integrin alphaVbeta3; D009842:Oligopeptides; C047981:arginyl-glycyl-aspartic acid; D007208:Indocyanine Green
keywords: fluorescence molecular imaging; gastric cancer; integrin; peritoneal carcinomatosis