title: [Overexpression of liver kinase B1 inhibits the proliferation of lung cancer cells].
abstract: Objective To explore the effect of overexpressed liver kinase B1(LKB1) on the proliferation of lung cancer cell lines. Methods The expression levels of LKB1 and PTEN in A549, NCI-H23, NCI-H157, XWLC-05, NCI-H446 lung cancer cells were detected by immunocytochemistry (ICC) and Western blotting. Plasmid pcDNA3.1+-LKB1 and empty vector pcDNA3.1+-null were separately transfected into the above five cell lines, and then the expression of LKB1 mRNA and protein were determined by quantitative real-time PCR and Western blotting, respectively. Finally, CCK-8 assay was used to analyze the proliferation ability of the transfected cells. Results LKB1 and PTEN were positive in NCI-H23 cells; LKB1 was negative while PTEN was positive in A549 and NCI-H446 cells; both LKB1 and PTEN were negative in NCI-H157 and XWLC-05 cells. Quantitative real-time PCR and Western blotting showed that the expression level of LKB1 significantly increased in the above cell lines transfected with plasmid pcDNA3.1+-LKB1 compared with the ones with empty vector pcDNA3.1+-null. Besides, CCK-8 assay showed that the overexpression of LKB1 in the lung cancer cells transfected with pcDNA3.1+-LKB1 had an obvious inhibitory effect on cell proliferation. Conclusion The expression of LKB1 is down-regulated in most of the lung cell lines to different extent and the over-expression of LKB1 can remarkably inhibit the proliferation ability of lung cancer cell lines.
authors.affiliation: Medical College, Kunming University of Science and Technology, Department of Thoracic Surgery, Hospital Affiliated to Kunming University of Science and Technology, First People's Hospital of Yunnan Province, Kunming 650500, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Thoracic Surgery, Hospital Affiliated to Kunming University of Science and Technology, First People's Hospital of Yunnan Province, Kunming 650500, China.
authors.forename: Libin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Department of Thoracic Surgery, Hospital Affiliated to Kunming University of Science and Technology, First People's Hospital of Yunnan Province, Kunming 650500, China. *Corresponding author, E-mail: wangping2467@aliyun.com.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D000072283:A549 Cells; D000091162:AMP-Activated Protein Kinase Kinases; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics*; D015536:Down-Regulation / Q000235:genetics; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics*; D051059:PTEN Phosphohydrolase / Q000235:genetics; D017346:Protein Serine-Threonine Kinases / Q000235:genetics*
chemical_list: D017346:Protein Serine-Threonine Kinases; C109790:STK11 protein, human; D000091162:AMP-Activated Protein Kinase Kinases; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human