title: Anti-HBV Activities of Three Compounds Extracted and Purified from Herpetospermum Seeds.
abstract: The goal of this research was to evaluate the anti-hepatitis B virus (HBV) activities of three compounds extracted and purified fromHerpetospermumseeds (HS) on HepG2.2.15 cells. Herpetin (HPT), herpetone (HPO), and herpetfluorenone (HPF) were isolated from HS and identified using HR-ESI-MS and NMR. Different concentrations of the drugs were added to the HepG2.2.15 cells. Cell toxicity was observed with an MTT assay, cell culture supernatants were collected, and HBsAg and HBeAg were detected by ELISA. The content of HBV DNA was determined via quantitative polymerase chain reaction (PCR) with fluorescent probes. The 50% toxicity concentration (TC50) of HPF was 531.48 μg/mL, suggesting that this species is less toxic than HPT and HPO. HPT and HPF showed more potent antiviral activities than HPO. The 50% inhibition concentration (IC50) values of HPF on HBsAg and HBeAg were 176.99 and 134.53 μg/mL, respectively, and the corresponding therapeutic index (TI) values were 2.66 and 3.49, respectively. HPT and HPF were shown to significantly reduce the level of HBV DNA in the HepG2.2.15 culture medium compared to the negative control. This initial investigation of the anti-HBV constituents of HS yielded three compounds that revealed a synergistic effect of multiple components in the ethnopharmacological use of HS.
authors.affiliation: College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China. gongpuyang1990@163.com.
authors.forename: Pu-Yang
authors.initials: PY
authors.lastname: Gong
authors.affiliation: Department of Pharmacy, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. zhixiang-yuan@hotmail.com.
authors.forename: Zhi-Xiang
authors.initials: ZX
authors.lastname: Yuan
authors.affiliation: College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China. gujian_3@163.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Gu
authors.affiliation: College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China. tanrui@home.swjtu.edu.cn.
authors.forename: Rui
authors.initials: R
authors.lastname: Tan
authors.affiliation: College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China. ligat@163.com.
authors.forename: Jia-Chuan
authors.initials: JC
authors.lastname: Li
authors.affiliation: College of Pharmacy, Southwest University for Nationalities, Chengdu 610041, Sichuan, China. renyan@swun.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Ren
authors.affiliation: College of Medicine, Southwest Jiaotong University, Chengdu 610031, Sichuan, China. hs20091688@sina.com.
authors.forename: Sha
authors.initials: S
authors.lastname: Hu
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology*; D001572:Benzofurans / Q000494:pharmacology*; D045744:Cell Line, Tumor; D019662:Cucurbitaceae / Q000737:chemistry; D004279:DNA, Viral / Q000235:genetics; D004365:Drugs, Chinese Herbal / Q000737:chemistry; D004797:Enzyme-Linked Immunosorbent Assay; D005449:Fluorenes / Q000494:pharmacology*; D056945:Hep G2 Cells; D006509:Hepatitis B / Q000188:drug therapy; D006514:Hepatitis B Surface Antigens / Q000378:metabolism*; D006513:Hepatitis B e Antigens / Q000378:metabolism*; D006515:Hepatitis B virus / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D017705:Lignans / Q000494:pharmacology*; D019906:Nuclear Magnetic Resonance, Biomolecular; D010936:Plant Extracts / Q000494:pharmacology; D012639:Seeds / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization; D014779:Virus Replication / Q000187:drug effects
chemical_list: D000998:Antiviral Agents; D001572:Benzofurans; D004279:DNA, Viral; D004365:Drugs, Chinese Herbal; D005449:Fluorenes; D006514:Hepatitis B Surface Antigens; D006513:Hepatitis B e Antigens; D017705:Lignans; D010936:Plant Extracts; C000617871:herpetfluorenone; C000617851:herpetin; C526382:herpetone
keywords: HepG2.2.15 cells; Herpetospermum seeds; hepatitis B virus