title: MicroRNA-187 exerts tumor-suppressing functions in osteosarcoma by targeting ZEB2.
abstract: MicroRNA-187 (miR-187) has been reported to be involved in the occurrence and development of several types of cancers; however, a role for miR-187 in osteosarcoma (OS) has not yet been reported. Here, miR-187 was found to be significantly downregulated in OS cell lines and tissue samples, and decreased miR-187 expression was shown to be correlated closely with the TNM stage and lymph node metastasis. miR-187 overexpression suppressed OS cell proliferation, colony formation, migration, invasion, and epithelial-mesenchymal transition (EMT). Mechanically, zinc finger E-box binding homeobox 2 (ZEB2) was shown to serve as a direct target of miR-187 in OS cells and the overexpression ofZEB2rescued the miR-187-induced suppression of proliferation, colony formation, migration, and invasion in OS cells. In clinical OS specimens,ZEB2expression levels were elevated and were inversely correlated with miR-187 expression. These results suggest that miR-187 functions as a tumor suppressor in OS, partially by targetingZEB2, and that miR-187 can serve as a promising candidate for OS.
authors.affiliation: Department of Ultrasonographic, China-Japan Union Hospital of Jilin University Changchun 130033, Jilin, P. R. China.
authors.forename: Dan
authors.initials: D
authors.lastname: Fei
authors.affiliation: Department of Orthopaedic Surgery, China-Japan Union Hospital of Jilin University Changchun 130033, Jilin, P. R. China.
authors.forename: Kunchi
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Nephrology, The Fourth Hospital of Jilin University Changchun 130000, Jilin, P. R. China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Yuan
authors.affiliation: Department of Ultrasonographic, China-Japan Union Hospital of Jilin University Changchun 130033, Jilin, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Xing
authors.affiliation: Department of Orthopaedic Surgery, China-Japan Union Hospital of Jilin University Changchun 130033, Jilin, P. R. China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Zhao
keywords: Osteosarcoma; ZEB2; miR-187; proliferation