title: Scavenger Receptor Class B, Type I, a CD36 Related Protein inMacrobrachium nipponense: Characterization, RNA Interference, and Expression Analysis with Different Dietary Lipid Sources.
abstract: The scavenger receptor class B, type I (SR-BI), is a member of the CD36 superfamily comprising transmembrane proteins involved in mammalian and fish lipid homeostasis regulation. We hypothesize that this receptor plays an important role inMacrobrachium nipponenselipid metabolism. However, little attention has been paid to SR-BI in commercial crustaceans. In the present study, we report a cDNA encodingM. nipponensescavenger receptor class B, type I (designated as MnSR-BI), obtained from a hepatopancreas cDNA library. The complete MnSR-BI coding sequence was 1545 bp, encoding 514 amino acid peptides. The MnSR-BI primary structure consisted of a CD36 domain that contained two transmembrane regions at the N- and C-terminals of the protein. SR-BI mRNA expression was specifically detected in muscle, gill, ovum, intestine, hepatopancreas, stomach, and ovary tissues. Furthermore, its expression in the hepatopancreas was regulated by dietary lipid sources, with prawns fed soybean and linseed oils exhibiting higher expression levels. RNAi-based SR-BI silencing resulted in the suppression of its expression in the hepatopancreas and variation in the expression of lipid metabolism-related genes. This is the first report of SR-BI in freshwater prawns and provides the basis for further studies on SR-BI in crustaceans.
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, CAFS, College of Life Science, Huzhou University, Huzhou, Zhejiang 313000, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Ding
authors.affiliation: College of Fisheries and Life Science, Dalian Ocean University, Dalian 116000, China.
authors.forename: Na
authors.initials: N
authors.lastname: Luo
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, CAFS, College of Life Science, Huzhou University, Huzhou, Zhejiang 313000, China.
authors.forename: Youqin
authors.initials: Y
authors.lastname: Kong
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, CAFS, College of Life Science, Huzhou University, Huzhou, Zhejiang 313000, China.
authors.forename: Jingfen
authors.initials: J
authors.lastname: Li
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, CAFS, College of Life Science, Huzhou University, Huzhou, Zhejiang 313000, China.
authors.forename: Yixiang
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, CAFS, College of Life Science, Huzhou University, Huzhou, Zhejiang 313000, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Cao
authors.affiliation: Zhejiang Provincial Key Laboratory of Aquatic Resources Conservation and Development, Key Laboratory of Aquatic Animal Genetic Breeding and Nutrition, CAFS, College of Life Science, Huzhou University, Huzhou, Zhejiang 313000, China.
authors.forename: Jinyun
authors.identifier: 0000-0002-8816-8312
authors.initials: J
authors.lastname: Ye