title: Dietary Wolfberry Extract Modifies Oxidative Stress by Controlling the Expression of Inflammatory mRNAs in Overweight and Hypercholesterolemic Subjects: A Randomized, Double-Blind, Placebo-Controlled Trial.
abstract: In the present study, we evaluated the antioxidative and anti-inflammatory effects of an aqueous extract of wolfberry fruit (WBE) in mild hypercholesterolemic and overweight subjects. This study was a double-blind randomized trial of two parallel groups of free-living subjects (n = 53). The participants consumed the contents of an 80 mL pouch containing 13.5 g WBE or placebo after one meal per day over an 8-week period. Following 8 weeks of WBE supplementation, we observed a slight but significant decrease in erythrocyte superoxide dismutase activity and an increase in catalase activity. Furthermore, to assess endogenous DNA damage in lymphocytes, the alkaline comet assay was performed, showing that the percentage of DNA in the tail was significantly decreased by 8-week WBE intake. Additionally, the proportion of significantly deregulated mRNAs related to oxidative or inflammatory stress was considerably higher in the WBE intake group. The present data indicate that WBE intake has antioxidative and anti-inflammatory effects in overweight and hypercholesterolemic subjects by modulating mRNA expression.
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University , Seoul 120-750, Republic of Korea.
authors.forename: You Jin
authors.initials: YJ
authors.lastname: Lee
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University , Seoul 120-750, Republic of Korea.
authors.forename: Youngsook
authors.initials: Y
authors.lastname: Ahn
authors.affiliation: Department of Nutritional Science and Food Management, Ewha Womans University , Seoul 120-750, Republic of Korea.
authors.forename: Oran
authors.initials: O
authors.lastname: Kwon
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University , Seoul 05029, Republic of Korea.
authors.forename: Mee Youn
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Department of Bioscience and Biotechnology, Konkuk University , Seoul 05029, Republic of Korea.
authors.forename: Choong Hwan
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Interdisciplinary Program in Bioinformatics, Seoul National University , Seoul 151-747, Republic of Korea.
authors.forename: Sungyoung
authors.initials: S
authors.lastname: Lee
authors.affiliation: Interdisciplinary Program in Bioinformatics, Seoul National University , Seoul 151-747, Republic of Korea.|Department of Statistics, Seoul National University , Seoul 151-747, Republic of Korea.
authors.forename: Taesung
authors.initials: T
authors.lastname: Park
authors.affiliation: Department of Surgery, CHA Bundang Medical Center, CHA University , Seongnam, Gyeonggi-do 463-712, Republic of Korea.
authors.forename: Sung Won
authors.initials: SW
authors.lastname: Kwon
authors.affiliation: Department of Food Science and Technology, Seoul National University of Science and Technology , Seoul 139-743, Republic of Korea.
authors.forename: Ji Yeon
authors.identifier: http://orcid.org/0000-0002-4316-2726
authors.initials: JY
authors.lastname: Kim
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000627:therapeutic use; D000975:Antioxidants / Q000378:metabolism; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D006937:Hypercholesterolemia / Q000150:complications / Q000178:diet therapy*; D007249:Inflammation / Q000178:diet therapy / Q000209:etiology / Q000235:genetics; D032305:Lycium / Q000737:chemistry*; D008297:Male; D008875:Middle Aged; D050177:Overweight / Q000150:complications / Q000178:diet therapy*; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000737:chemistry / Q000627:therapeutic use*; D019518:Postprandial Period
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D010936:Plant Extracts
keywords: DNA damage; inflammation; mRNA array; postprandial inflammatory response; wolfberry fruit extract