title: A highly sensitive signal-amplified gold nanoparticle-based electrochemical immunosensor for dibutyl phthalate detection.
abstract: A simple and signal-amplified, label-free electrochemical impedimetric immunosensor for dibutyl phthalate (DBP), a type of phthalate ester, was developed using gold nanoparticles (AuNPs) induced signal amplification. The approach was based on an indirectly competitive binding system of coating antigen that coated on antigen/chitosan/MWCNTs@GONRs/GCE (modified electrodes), target (DBP) and anti-DBP antibody. Signal amplification was performed via the enlargement of AuNPs through NADH-promoted catalytic precipitation, which caused a marked increase in the electron-transfer resistance and electrostatic repulsion after an AuNP-labeled second antibody conjugated to anti-DBP. Under the optimized conditions, this method displayed: (i) low detection limits (7ng/mL, 10 times lower than the traditional ELISA method using the same antibody); (ii) satisfactory accuracy (recoveries, 86.0-120.4%) and agreement with corresponding ELISA method; (iii) high tolerance to some environmental interferents; and (iv) low cost and low sample consumption (6µL). Our results demonstrate the great potential and high efficiency of this immunosensor in practical applications for the cost-effective and sensitive monitoring of DBP in aquatic environments.
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Ya-Ru
authors.initials: YR
authors.lastname: Liang
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: yunm998@126.com.
authors.forename: Zong-Mian
authors.initials: ZM
authors.lastname: Zhang
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhen-Jiang
authors.initials: ZJ
authors.lastname: Liu
authors.affiliation: The School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Wang
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Xiang-Yang
authors.initials: XY
authors.lastname: Wu
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zeng
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Meng
authors.affiliation: School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China. Electronic address: zhangzhen@ujs.edu.cn.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Zhang
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000191:economics / Q000295:instrumentation; D048271:Chitosan / Q000737:chemistry; D003993:Dibutyl Phthalate / Q000032:analysis*; D055664:Electrochemical Techniques / Q000191:economics / Q000295:instrumentation*; D004566:Electrodes; D004784:Environmental Monitoring / Q000191:economics / Q000295:instrumentation; D006046:Gold / Q000737:chemistry*; D007118:Immunoassay / Q000191:economics / Q000295:instrumentation*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry*; D037742:Nanotubes, Carbon / Q000737:chemistry; D010968:Plasticizers / Q000032:analysis; D045483:Rivers / Q000737:chemistry*; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D055910:Antibodies, Immobilized; D037742:Nanotubes, Carbon; D010968:Plasticizers; D014874:Water Pollutants, Chemical; D003993:Dibutyl Phthalate; D006046:Gold; D048271:Chitosan
keywords: Antibody; Dibutyl Phthalate; Electrochemical Immunosensor; Gold Nanoparticles; Signal Amplification