title: Molecular and karyological data confirm that the enigmatic genus Platypholis from Bonin-Islands (SE Japan) is phylogenetically nested within Orobanche (Orobanchaceae).
abstract: Molecular phylogenetic studies have greatly improved our understanding of phylogenetic relationships of non-photosynthetic parasitic broomrapes (Orobanche and related genera, Orobanchaceae), but a few genera have remained unstudied. One of those is Platypholis, whose sole species, Platypholis boninsimae, is restricted to the Bonin-Islands (Ogasawara Islands) about 1000 km southeast of Japan. Based on overall morphological similarity, Platypholis has been merged with Orobanche, but this hypothesis has never been tested with molecular data. Employing maximum likelihood and Bayesian analyses on a family-wide data set (two plastid markers, matK and rps2, and three nuclear markers, ITS, phyA and phyB) as well as on an ITS data set focusing on Orobanche s. str., it is shown that P. boninsimae Maxim. is phylogenetically closely linked to or even nested within Orobanche s. str. This position is supported both by morphological evidence and by the newly obtained chromosome number of 2n = 38, which is characteristic for the genus Orobanche s. str.
authors.affiliation: Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030, Vienna, Austria.
authors.forename: Xi
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030, Vienna, Austria.
authors.forename: Tae-Soo
authors.initials: TS
authors.lastname: Jang
authors.affiliation: Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030, Vienna, Austria.
authors.forename: Eva M
authors.initials: EM
authors.lastname: Temsch
authors.affiliation: Makino Herbarium, Tokyo Metropolitan University, 1-1 Minami-Ohsawa, Hachioji-shi, Tokyo, 192-0397, Japan.
authors.forename: Hidetoshi
authors.initials: H
authors.lastname: Kato
authors.affiliation: Museum of Natural and Environmental History, Shizuoka, 5762 Oya, Suruga-ku, Shizuoka-shi, Shizuoka, 422-8017, Japan.
authors.forename: Koji
authors.initials: K
authors.lastname: Takayama
authors.affiliation: Department of Botany and Biodiversity Research, University of Vienna, Rennweg 14, 1030, Vienna, Austria. gerald.schneeweiss@univie.ac.at.
authors.forename: Gerald M
authors.initials: GM
authors.lastname: Schneeweiss
mesh_terms: D002467:Cell Nucleus / Q000235:genetics; D060365:Chloroplast Proteins / Q000235:genetics; D032461:Chromosomes, Plant; D021903:DNA, Ribosomal Spacer / Q000235:genetics*; D018745:Genome, Plant; D031670:Orobanchaceae / Q000235:genetics*; D010802:Phylogeny; D010940:Plant Proteins / Q000235:genetics*; D017422:Sequence Analysis, DNA
chemical_list: D060365:Chloroplast Proteins; D021903:DNA, Ribosomal Spacer; D010940:Plant Proteins
keywords: Bonin Islands; Chromosome number; Molecular phylogeny; Orobanche; Parasitic plant; Platypholis