title: Enhanced hydrogen production of Enterobacter aerogenes mutated by nuclear irradiation.
abstract: Nuclear irradiation was used for the first time to generate efficient mutants of hydrogen-producing bacteria Enterobacter aerogenes, which were screened with larger colour circles of more fermentative acid by-products. E. aerogenes cells were mutated by nuclear irradiation of60Co γ-rays. The screened E. aerogenes ZJU1 mutant with larger colour circles enhanced the hydrogenase activity from 89.8 of the wild strain to 157.4mLH2/(gDWh). The hereditary stability of the E. aerogenes ZJU1 mutant was certified after over ten generations of cultivation. The hydrogen yield of 301mLH2/gglucose with the mutant was higher by 81.8% than that of 166mL/gglucose with the wild strain. The peak hydrogen production rate of 27.2mL/(L·h) with the mutant was higher by 40.9% compared with that of 19.3mL/(L·h) with the wild strain. The mutant produced more acetate and butyrate but less ethanol compared with the wild strain during hydrogen fermentation.
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China. Electronic address: juncheng@zju.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Cheng
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China; Department of Life Science and Engineering, Jining University, Jining 273155, China.
authors.forename: Wenlu
authors.initials: W
authors.lastname: Song
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Lingkan
authors.initials: L
authors.lastname: Ding
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Liu
authors.affiliation: Shandong Botong New Energy Company, Jining 272000, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Clean Energy Utilization, Zhejiang University, Hangzhou 310027, China.
authors.forename: Kefa
authors.initials: K
authors.lastname: Cen
mesh_terms: D021902:Enterobacter aerogenes / Q000235:genetics / Q000378:metabolism* / Q000528:radiation effects*; D005285:Fermentation; D005720:Gamma Rays; D006859:Hydrogen / Q000737:chemistry; D009154:Mutation / Q000528:radiation effects
chemical_list: D006859:Hydrogen
keywords: Bacteria mutant; Enterobacter aerogenes; Hydrogen; Nuclear irradiation