title: Isolation of CHS Gene from Brunfelsia acuminata Flowers and Its Regulation in Anthocyanin Biosysthesis.
abstract: Chalcone synthase gene (BaCHS) fromBrunfelsia acuminataflowers was isolated using RT-PCR and RACE. The coding region of the gene is 1425-bp with an open reading frame of 1170-bp, 73-bp 5'UTR, and 172-bp 3'UTR. Its deduced protein does not have a signal peptide but does contain a cond_enzyme superfamily domain, and consists of 389 amino acids with a predicted molecular mass of 42,699 Da and a pI of 6.57. The deduced amino acid sequence of BaCHS shares 90%, 88%, 85%, 84% and 79% identity with CHS fromPetunia hybrida,Nicotiana tabacum,Solanum lycopersicum,Capsicum annuumandCamellia sinensis, respectively. The striking color change from dark purple to light purple and ultimately lead to pure white resulted from a decline in anthocyanin content of the petals and was preceded by a decrease in the expression ofBaCHS. Its gene expression was positively correlated with the contents of anthocyanin (p≤ 0.01).
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. liminzyl@sina.com.
authors.forename: Min
authors.initials: M
authors.lastname: Li
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. caoyt87@163.com.
authors.forename: Yu-Ting
authors.initials: YT
authors.lastname: Cao
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. yesirui@163.com.
authors.forename: Si-Rui
authors.initials: SR
authors.lastname: Ye
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. irshadaup@gmail.com.
authors.forename: Muhammad
authors.initials: M
authors.lastname: Irshad
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. tfpan@fafu.edu.cn.
authors.forename: Teng-Fei
authors.initials: TF
authors.lastname: Pan
authors.affiliation: College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China. qiudl1970@fafu.edu.cn.
authors.forename: Dong-Liang
authors.initials: DL
authors.lastname: Qiu
mesh_terms: D000217:Acyltransferases / Q000235:genetics*; D000595:Amino Acid Sequence; D000872:Anthocyanins / Q000096:biosynthesis*; D001483:Base Sequence; D028241:Camellia sinensis / Q000235:genetics; D002212:Capsicum / Q000235:genetics; D035264:Flowers / Q000235:genetics* / Q000378:metabolism; D018551:Solanum lycopersicum / Q000378:metabolism; D032306:Petunia / Q000235:genetics*; D010858:Pigmentation / Q000235:genetics*; D016415:Sequence Alignment; D017422:Sequence Analysis, DNA; D017386:Sequence Homology, Amino Acid; D014026:Nicotiana / Q000235:genetics
chemical_list: D000872:Anthocyanins; D000217:Acyltransferases; C020141:flavanone synthetase
keywords: Brunfelsia acuminata; RT-qPCR; anthocyanin biosynthesis; chalcone synthase (CHS); flower color