title: Comparison of quinolone and beta-lactam resistance among Escherichia coli strains isolated from urinary tract infections.
abstract: The growing frequency of antibiotic resistances is now a universal problem. Increasing resistance to new generations of β-lactam and quinolone antibiotics in multidrug-resistant Enterobacteriaceae isolates is considered an emergency health issue worldwide. The aim of this study was to evaluate plasmid-mediated quinolone resistance genes in ESBL-producing Escherichia coli isolated from urinary tract infections (UTIs). In our study ESBL-producing isolates were assessed by screening methods. After determination of antimicrobial susceptibility testing, detection of ESBLs and quinolone resistance genes was performed. A total of 97 ESBL-producing E. coli were determined. The bla-TEM, bla-SHV and bla-CTX-M genes were detected in 90 isolates. The bla-TEM was the most frequently detected gene (46.4%), followed by bla-SHV (31.9%) and bla-CTX-M (14.4%). The most prevalent quinolone resistance gene among ESBL-producing isolates was oqxAB which was found in 67 isolates (69.1%). The frequencies of the aac(6')-Ib-cr, qnr and qepA were 65 (67%), 8 (8.2%) and 6 (6.2%), respectively. Our data indicate that the prevalence of plasmid-mediated quinolone resistance genes in ESBL-positive isolates is increasing. The co-dissemination of PMQR and ESBL genes among E. coli isolates can be considered a threat to public health. Therefore, prescription of antibiotics against infectious disease should be managed carefully.
authors.affiliation: Microbiology Department, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Tayebi
authors.affiliation: Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
authors.forename: Hamid
authors.initials: H
authors.lastname: Heidari
authors.affiliation: Department of Medical Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran; Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran.
authors.forename: Hossein
authors.initials: H
authors.lastname: Kazemian
authors.affiliation: Microbiology Department, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran.
authors.forename: Seyed Mohammad
authors.initials: SM
authors.lastname: Ghafoori
authors.affiliation: Microbiology Department, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran; Pars Advance and Minimally Invasive Research Center, Pars hospital, Iran University of Medical Sciences, Tehran, Iran.
authors.forename: Shahram
authors.initials: S
authors.lastname: Boroumandi
authors.affiliation: Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Hamidreza
authors.initials: H
authors.lastname: Houri
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D003430:Cross-Sectional Studies; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics; D004926:Escherichia coli / Q000235:genetics* / Q000302:isolation & purification; D004927:Escherichia coli Infections / Q000150:complications* / Q000453:epidemiology; D005838:Genotype; D006761:Hospitals / Q000706:statistics & numerical data; D006801:Humans; D007297:Inpatients / Q000706:statistics & numerical data; D007492:Iran / Q000453:epidemiology; D008826:Microbial Sensitivity Tests; D010045:Outpatients / Q000706:statistics & numerical data; D015995:Prevalence; D015363:Quinolones / Q000494:pharmacology; D014552:Urinary Tract Infections / Q000453:epidemiology / Q000382:microbiology*; D018440:beta-Lactam Resistance / Q000235:genetics*; D001618:beta-Lactamases / Q000032:analysis / Q000235:genetics*; D047090:beta-Lactams / Q000494:pharmacology
chemical_list: D000900:Anti-Bacterial Agents; D015363:Quinolones; D047090:beta-Lactams; D001618:beta-Lactamases