title: Methicillin-resistant staphylococcus aureus isolates in a hospital of shanghai.
abstract: Methicillin-resistant Staphylococcus aureus (MRSA) strains are now common both in the health care setting and in the community. Active surveillance is critical for MRSA control and prevention. Specimens of patients (200 patients with 1119 specimens) as well as medical staff and hospital setting (1000 specimens) were randomly sampled in a level 2 hospital in Shanghai from September 2011 to August 2012. Isolation, cultivation and identification of S. aureus were performed. Totally, 67 S. aureus strains were isolated. 32 S. aureus strains were isolated from patient samples; 13 (13/32, 40.6%) of the 32 S. aureus isolates were MRSA; sputum sample and patients in the department of general internal medicine were the most frequent specimen and patient group for S. aureus strains isolation. Remaining 35 S. aureus strains were isolated from the medical staff and hospital setting; 20 (20/35, 57.1%) of the 35 S. aureus isolates were MRSA; specimens sampled from doctors and nurses' hands and nose and hospital facilities were the most frequent samples to isolate S. aureus. Resistant and virulent genes detection showed that, all 33 MRSA strains were mecA positive which accounts for 49.3% of the 67 S. aureus strains; 38 isolates were Panton-Valentine leukocidin (PVL) gene positive which accounts for 56.7% of the 67 S. aureus strains; and 17 (17/67, 25.4%) isolates are mecA and PVL genes dual positive. Multidrug-resistant strains of MRSA and PVL positive S. aureus are common in patients, medical staff and hospital setting, the potential health threat is worthy of our attention.
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Xiaoguang
authors.initials: X
authors.lastname: Wang
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Lin
authors.initials: L
authors.lastname: Ouyang
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Luo
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Jiqian
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Chiping
authors.initials: C
authors.lastname: Song
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Cuizhen
authors.initials: C
authors.lastname: Li
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Hongjing
authors.initials: H
authors.lastname: Yan
authors.affiliation: The Center for Disease Control and Prevention of Minghang District, Minghang District, Shanghai 201101, P.R. China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
mesh_terms: D001426:Bacterial Proteins / Q000235:genetics*; D001427:Bacterial Toxins / Q000235:genetics*; D005098:Exotoxins / Q000235:genetics*; D006225:Hand / Q000382:microbiology; D006801:Humans; D007956:Leukocidins / Q000235:genetics*; D055624:Methicillin-Resistant Staphylococcus aureus / Q000145:classification / Q000235:genetics / Q000302:isolation & purification*; D009666:Nose / Q000382:microbiology; D046915:Penicillin-Binding Proteins / Q000235:genetics*; D011159:Population Surveillance; D013183:Sputum / Q000382:microbiology; D013203:Staphylococcal Infections / Q000175:diagnosis*
chemical_list: D001426:Bacterial Proteins; D001427:Bacterial Toxins; D005098:Exotoxins; D007956:Leukocidins; C078284:Panton-Valentine leukocidin; D046915:Penicillin-Binding Proteins; C487789:mecA protein, Staphylococcus aureus
keywords: hospital-associated MRSA; mecA gene; methicillin-resistant staphylococcus aureus (MRSA); panton-valentine leukocidin (PVL) gene