title: Label-Free Quantitative Proteomic Analysis of Harmless and Pathogenic Strains of Infectious Microalgae, Prototheca spp.
abstract: Microalgae of the genusPrototheca(P.) spp are associated with rare algal infections of invertebrates termed protothecosis. Among the seven generally accepted species,P. zopfiigenotype 2 (GT2) is associated with a severe form of bovine mastitis whileP. blaschkeaecauses the mild and sub-clinical form of mastitis. The reason behind the infectious nature ofP. zopfiiGT2, while genotype 1 (GT1) remains non-infectious, is not known. Therefore, in the present study we investigated the protein expression level difference between the genotypes ofP. zopfiiandP. blaschkeae.Cells were cultured to the mid-exponential phase, harvested, and processed for LC-MS analysis. Peptide data was acquired on an LTQ Orbitrap Velos, raw spectra were quantitatively analyzed with MaxQuant software and matching with the reference database ofChlorella variabilisandAuxenochlorella protothecoidesresulted in the identification of 226 proteins. Comparison of an environmental strain with infectious strains resulted in the identification of 51 differentially expressed proteins related to carbohydrate metabolism, energy production and protein translation. The expression level of Hsp70 proteins and their role in the infectious process is worth further investigation. All mass spectrometry data are available via ProteomeXchange with identifier PXD005305.
authors.affiliation: Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany. jayaseelan.murugaiyan@fu-berlin.de.
authors.forename: Jayaseelan
authors.initials: J
authors.lastname: Murugaiyan
authors.affiliation: Institute for Chemistry and Biochemistry, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany. Murat.eravci@gmx.de.
authors.forename: Murat
authors.initials: M
authors.lastname: Eravci
authors.affiliation: Institute for Chemistry and Biochemistry, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany. chris.weise@biochemie.fu-berlin.de.
authors.forename: Christoph
authors.initials: C
authors.lastname: Weise
authors.affiliation: Institute of Animal Hygiene and Environmental Health, Centre for Infectious Medicine, Freie Universität Berlin, Robert-von-Ostertag-Str. 7-13, 14163 Berlin, Germany. Uwe.Roesler@fu-berlin.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Roesler
mesh_terms: D000465:Algorithms; D013058:Mass Spectrometry; D010940:Plant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D020543:Proteome / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D011525:Prototheca / Q000235:genetics / Q000378:metabolism* / Q000472:pathogenicity; D014774:Virulence / Q000235:genetics
chemical_list: D010940:Plant Proteins; D020543:Proteome
keywords: LTQ Orbitrap Velos mass spectrometer; MaxQuant; Perseus; Prototheca; comparative proteomic analysis; label free quantitative analysis; proteomics; protothecosis