title: Population structure and genetic diversity of Indian Major Carp, Labeo rohita (Hamilton, 1822) from three phylo-geographically isolated riverine ecosystems of India as revealed by mtDNA cytochrome b region sequences.
abstract: The population structure and genetic diversity of Rohu (Labeo rohita Hamilton, 1822) was studied by analysis of the partial sequences of mitochondrial DNA cytochrome b region. We examined 133 samples collected from six locations in three geographically isolated rivers of India. Analysis of 11 haplotypes showed low haplotype diversity (0.00150), nucleotide diversity (π) (0.02884) and low heterogeneity value (0.00374). Analysis of molecular variance (AMOVA) revealed the genetic diversity of L. rohita within population is very high than between the populations. The Fst scores (-0.07479 to 0.07022) were the indication of low genetic structure of L. rohita populations of three rivers of India. Conspicuously, Farakka-Bharuch population pair Fst score of 0.0000, although the sampling sites are from different rivers. The phylogenetic reconstruction of unique haplotypes revealed sharing of a single central haplotype (Hap_1) by all the six populations with a point mutations ranging from 1-25 nucleotides.
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Bijay Kumar
authors.initials: BK
authors.lastname: Behera
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Vishwamitra Singh
authors.initials: VS
authors.lastname: Baisvar
authors.affiliation: c ICAR-Central Coastal Agricultural Research Institute , Old Goa , India.
authors.forename: Swaraj Priyaranjan
authors.initials: SP
authors.lastname: Kunal
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Dharmendra Kumar
authors.initials: DK
authors.lastname: Meena
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Debarata
authors.initials: D
authors.lastname: Panda
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Sudip
authors.initials: S
authors.lastname: Pakrashi
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Prasenjit
authors.initials: P
authors.lastname: Paria
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Pronob
authors.initials: P
authors.lastname: Das
authors.affiliation: b ICAR-Central Inland Fisheries Research Institute, Regional Center , Guwahati , India.
authors.forename: Dibakar
authors.initials: D
authors.lastname: Bhakta
authors.affiliation: b ICAR-Central Inland Fisheries Research Institute, Regional Center , Guwahati , India.
authors.forename: Dipesh
authors.initials: D
authors.lastname: Debnath
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: Suvra
authors.initials: S
authors.lastname: Roy
authors.affiliation: a Fish Biotechnology Laboratory , ICAR-Central Inland Fisheries Research Institute , Kolkata , India.
authors.forename: V R
authors.initials: VR
authors.lastname: Suresh
authors.affiliation: d Fisheries Division- Indian Council of Agricultural Research , New Delhi , India.
authors.forename: J K
authors.initials: JK
authors.lastname: Jena
mesh_terms: D000818:Animals; D002347:Carps / Q000145:classification* / Q000235:genetics; D045303:Cytochromes b / Q000235:genetics*; D019143:Evolution, Molecular; D029941:Fish Proteins / Q000235:genetics; D014644:Genetic Variation; D005828:Genetics, Population; D006239:Haplotypes; D007194:India; D010802:Phylogeny; D017354:Point Mutation; D045483:Rivers; D017422:Sequence Analysis, DNA / Q000379:methods*
chemical_list: D029941:Fish Proteins; D045303:Cytochromes b
keywords: Genetic diversity; Labeo rohita; cytochrome b; mitochondrial DNA