title: Epidemiologic Analysis of Respiratory Viral Infections Mainly in Hospitalized Children and Adults in a Midwest University Medical Center After the Implementation of a 14-Virus Multiplex Nucleic Acid Amplification Test.
abstract: OBJECTIVE
  To investigate the etiology of viral respiratory tract infections mainly in hospitalized children and adults over a 12-month consecutive period after implementation of a 14-virus multiplex nucleic acid amplification test.
  
  
  METHODS
  From January 2014 to January 2015, a total of 2,237 respiratory samples were analyzed with the US Food and Drug Administration-cleared eSensor Respiratory Viral Panel (GenMark Diagnostics, Carlsbad, CA).
  
  
  RESULTS
  Of the 2,237 specimens tested, 788 specimens were positive for at least one virus, giving a positivity rate of 35.2%, and because of viral codetection, a total of 862 viral targets were identified. The age groups with the highest positivity rates were the 0- to 1-year (73.5%) and 2- to 6-year (78.4%) age groups. The overall viral codetection rate was 9.1%. Human rhinovirus (HRV) was the most prevalent respiratory virus found in children and adults. The peak of HRV seen in September 2014 represented a combination of HRV and enterovirus D68, 2014 epidemic respiratory infections.
  
  
  CONCLUSIONS
  The ability to detect a wider range of respiratory viruses gave us a better understanding of the etiology of respiratory infections in our population, particularly for HRV and enhanced our ability to detect viral coinfection.
authors.affiliation: From the Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.
authors.forename: Sophie
authors.initials: S
authors.lastname: Arbefeville
authors.affiliation: From the Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis.
authors.forename: Patricia
authors.initials: P
authors.lastname: Ferrieri
mesh_terms: D000046:Academic Medical Centers; D000293:Adolescent; D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D002648:Child; D002675:Child, Preschool; D005260:Female; D006760:Hospitalization; D006801:Humans; D007223:Infant; D007231:Infant, Newborn; D008297:Male; D008875:Middle Aged; D021141:Nucleic Acid Amplification Techniques; D015995:Prevalence; D012141:Respiratory Tract Infections / Q000453:epidemiology* / Q000821:virology*; D014777:Virus Diseases / Q000453:epidemiology*; D055815:Young Adult
keywords: Epidemiology; Etiology; Multiplex nucleic acid amplification; Respiratory viruses; Viral codetection