title: Behavior of nucleolus in the tobacco male meiocytes involved in cytomixis.
abstract: Behavior of nucleolus during the nuclear migration between plant cells (cytomixis) is studied for the first time in the tobacco male meiosis. As is shown, the nucleolus is located in a nonrandom manner in the migrating nuclei. In the majority of cases, the nucleolus resides on the nuclear pole strictly opposite to the cytomictic channel. Owing to this localization, the nucleolus extremely rare enters the recipient cell, so that the nucleolar material is in most cases undetectable in the micronuclei formed after cytomixis. When a whole nucleus migrates from a donor cell to recipient, the nucleolus can leave the nucleus and remain in the donor cells either alone or with a small amount of chromatin. The causes underlying a nonrandom location of the nucleolus in cytomictic cells are discussed. It is assumed that the nucleolar material contacts the cytoplasmic cytoskeleton, which prevents migration of the nucleolus into another cell within the nucleus. The potential use of cytomixis as a model for studying the nuclear motion is discussed.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Mursalimov
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
authors.forename: Yuriy
authors.initials: Y
authors.lastname: Sidorchuk
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Prospekt Lavrentyeva 10, Novosibirsk, 630090, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Deineko
mesh_terms: D002466:Cell Nucleolus / Q000502:physiology* / Q000648:ultrastructure; D035264:Flowers; D008540:Meiosis / Q000502:physiology*; D059828:Plant Cells / Q000502:physiology* / Q000648:ultrastructure; D010936:Plant Extracts / Q000302:isolation & purification; D014026:Nicotiana / Q000166:cytology* / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D010936:Plant Extracts
keywords: Nicotiana tabacum; cytomixis; fibrillarin; meiosis; nucleolus