title: Physicochemical, antioxidant and biocompatible properties of chondroitin sulphate isolated from chicken keel bone for potential biomedical applications.
abstract: Chicken keel bone cartilage was explored for cheaper and sustainable source for isolation of chondroitin sulphate (CS) for its future use in tissue engineering and pharmaceutical industry. HPSEC analysis displayed two peaks of 100kDa for CS-keel polysaccharide and 1kDa for protein. DLS analysis of CS-keel displayed polydispersity. CS-keel yield was 15% and 53±5% uronic acid content. The quantified percentages of UA-GalNAc4S and UA-GalNAc6S disaccharide in CS-keel were 58% and 42%, respectively. FT-IR identified CS-keel to be chondroitin 4-sulphate.1H NMR of CS-keel confirmed the presence of N-acetylgalactosamine and Glucuronic acid. FESEM demonstrated layer structure and AFM displayed the size of CS-keel fibres. DSC, TGA and DTG studies of CS-keel showed Tdat 243°C. In vitro cell proliferation assay and morphological analysis of mouse fibroblast L929 cell lines confirmed the biocompatibility of CS-keel. CS-keel (5mg/ml) exhibited ∼49% antioxidant activity against DPPH and 22% against superoxide radical protecting from oxidative damage. CS-keel demonstrated better (70.3%) emulsifying activity than commercial sodium alginate (60.2%).
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: Aruna
authors.initials: A
authors.lastname: Rani
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.
authors.forename: Rwivoo
authors.initials: R
authors.lastname: Baruah
authors.affiliation: Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: arungoyl@iitg.ernet.in.
authors.forename: Arun
authors.initials: A
authors.lastname: Goyal
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology; D002356:Cartilage / Q000737:chemistry*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D002809:Chondroitin Sulfates / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D005347:Fibroblasts / Q000187:drug effects*; D051379:Mice; D017550:Spectroscopy, Fourier Transform Infrared; D013481:Superoxides / Q000737:chemistry
chemical_list: D000975:Antioxidants; D001672:Biocompatible Materials; D013481:Superoxides; D002809:Chondroitin Sulfates
keywords: Antioxidant activity; Chicken keel bone cartilage; Chondroitin sulphate; DSC; MTT assay; TGA