title: An efficient ratiometric fluorescence sensor based on metal-organic frameworks and quantum dots for highly selective detection of 6-mercaptopurine.
abstract: The development of a simple and accurate quantitative method for the determination of 6-mercaptopurine (6-MP) is of great importance because of its serious side effects. Ratiometric fluorescence (RF) sensors are not subject to interference from environmental factors, and exhibit enhanced precision and accuracy. Therefore, a novel RF sensor for the selective detection of 6-MP was developed based on a dual-emission nanosensor. The nanosensor was fabricated by combining a blue-emission metal-organic framework (MOF) NH2-MIL-53(Al) (λem=425nm) with green-emission 3-mercaptopropionic acid-capped CdTe quantum dots (MPA-CdTe QDs) (λem=528nm) under a single excitation wavelength (335nm). Upon addition of 6-MP, the fluorescence of NH2-MIL-53(Al) in the nanohybrid was selectively quenched due to strong inner filter effects, while the fluorescence of the MPA-CdTe QDs was enhanced. The novel RF sensor exhibited higher selectivity towards 6-MP than CdTe QDs alone, and higher sensitivity than MOFs alone. 6-MP could be detected in the range of 0-50μM with a detection limit of 0.15μM (S/N=3). The developed sensor was applied for the determination of 6-MP in human urine samples and satisfactory results were obtained. Overall, a novel and efficient fluorescence-based method was developed for the detection of 6-MP in biosamples.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Jin
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Zhao-Li
authors.initials: ZL
authors.lastname: Mou
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Rui-Ling
authors.initials: RL
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China.
authors.forename: Si-Si
authors.initials: SS
authors.lastname: Liang
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Key Laboratory of Medicinal Resource and Natural Pharmaceutical Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, China. Electronic address: zqzhang@snnu.edu.cn.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D015097:3-Mercaptopropionic Acid / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D019187:Cadmium Compounds / Q000737:chemistry*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D015122:Mercaptopurine / Q000652:urine*; D009942:Organometallic Compounds / Q000737:chemistry*; D045663:Quantum Dots / Q000737:chemistry* / Q000648:ultrastructure; D013050:Spectrometry, Fluorescence / Q000379:methods*; D013691:Tellurium / Q000737:chemistry*
chemical_list: D019187:Cadmium Compounds; D005456:Fluorescent Dyes; D009942:Organometallic Compounds; D015097:3-Mercaptopropionic Acid; D015122:Mercaptopurine; D013691:Tellurium; C028337:cadmium telluride
keywords: 6-Mercaptopurine; CdTe quantum dots; Inner filter effect; Metal-organic frameworks; Ratiometric fluorescence sensor