title: Development, characterization and biocompatibility of chondroitin sulfate/poly(vinyl alcohol)/bovine bone powder porous biocomposite.
abstract: Chondroitin sulfate (ChS), a sulfated glycosaminoglycan, poly(vinyl alcohol) (PVA) and bovine bone powder (BBP) were blended to form a novel eco-friendly biocomposite through cyclic freeze-thawing under mild conditions. The systematic investigation reveals that the content of BBP has a remarkable effect on the pore size, porosity, mechanical and liquid uptake properties and biodegradability. At 10wt.% BBP the biocomposite exhibited enhanced mechanical properties and biodegradability rate as compared to the pristine sample. Further, different properties of the biocomposite can be tailored according to the content of BBP. In vitro assays showed that ChS/PVA-BBP does not exert cytotoxicity against healthy cells. In vivo and ex vivo experiments revealed that ChS/PVA-BBP biocomposites are biocompatibility materials without exert pro-inflammatory responses. The biocomposite was completely biodegraded and bioresorbed after 15days of treatment. Taken together, BBP is a low-cost source of hydroxyapatite and collagen, which are insurance. All these results suggest that the biocomposite designed in this study is a promising biomaterial for potential skin tissue engineering.
authors.affiliation: Laboratório de Tecnologia e Desenvolvimento de Materiais Poliméricos e Compósitos - LaCoPol. Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil.
authors.forename: Gabriela T
authors.initials: GT
authors.lastname: da Silva
authors.affiliation: Grupo de Pesquisa em Neurobiotecnologia (GPN). Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil.
authors.forename: Guilherme T
authors.initials: GT
authors.lastname: Voss
authors.affiliation: Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos and Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Universidade Estadual de Maringá (UEM), 87020-900 Maringá, Paraná, Brazil.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Kaplum
authors.affiliation: Laboratório de Microbiologia Aplicada aos Produtos Naturais e Sintéticos and Laboratório de Inovação Tecnológica no Desenvolvimento de Fármacos e Cosméticos, Universidade Estadual de Maringá (UEM), 87020-900 Maringá, Paraná, Brazil.
authors.forename: Celso V
authors.initials: CV
authors.lastname: Nakamura
authors.affiliation: Grupo de Pesquisa em Neurobiotecnologia (GPN). Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil.
authors.forename: Ethel A
authors.initials: EA
authors.lastname: Wilhelm
authors.affiliation: Grupo de Pesquisa em Neurobiotecnologia (GPN). Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil.
authors.forename: Cristiane
authors.initials: C
authors.lastname: Luchese
authors.affiliation: Laboratório de Tecnologia e Desenvolvimento de Materiais Poliméricos e Compósitos - LaCoPol. Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil. Electronic address: andre.fajardo@pq.cnpq.br.
authors.forename: André R
authors.initials: AR
authors.lastname: Fajardo
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity; D001842:Bone and Bones / Q000737:chemistry*; D002417:Cattle; D002470:Cell Survival / Q000187:drug effects; D002809:Chondroitin Sulfates / Q000737:chemistry*; D055119:Elastic Modulus; D008297:Male; D051379:Mice; D008855:Microscopy, Electron, Scanning; D011142:Polyvinyl Alcohol / Q000737:chemistry*; D016062:Porosity; D013052:Spectrometry, X-Ray Emission; D017550:Spectroscopy, Fourier Transform Infrared; D013818:Thermogravimetry; D023822:Tissue Engineering; D014961:X-Ray Diffraction
chemical_list: D001672:Biocompatible Materials; D011142:Polyvinyl Alcohol; D002809:Chondroitin Sulfates
keywords: Biocomposites; Biomaterial; Chondroitin sulfate; Composites; Porous materials; Tissue engineering