title: Parkinsonism-associated protein DJ-1 is a bona fide deglycase.
abstract: We discovered recently that Parkinsonism-associated DJ-1 and its bacterial homologs function as protein deglycases that repair glyoxal- and methylglyoxal-glycated proteins. Protein glycation levels are 2- to 10-fold increased in deglycase-depleted cells, and deglycase mutants display up to 500-fold loss of viability in methylglyoxal or glucose-containing media, suggesting that these deglycases play important roles in protecting cells against electrophile and carbonyl stress. Although the deglycase activity of DJ-1 is well supported by extensive biochemical work, Pfaff et al. (J. Biol. Chem. in presshttp://dx.doi.org/10.1074/jbc.M116.743823) claimed in a recent study that deglycation of the hemithioacetal formed upon cysteine glycation by methylglyoxal results from a Tris buffer artefact. Here, we show that this is not the case, and that DJ-1 and its homologs are the bona fide deglycases awaited since the Maillard discovery.
authors.affiliation: Stress Molecules, Institut Jacques Monod, Université Paris 7, CNRS UMR 7592, 15 Rue Hélène Brion, 75013, Paris, France. Electronic address: richarme@paris7.jussieu.fr.
authors.forename: Gilbert
authors.initials: G
authors.lastname: Richarme
authors.affiliation: UMR 8601 CNRS, Laboratoire de Chimie et Biochimie Pharmacologiques et Toxicologiques, Université Paris Descartes-Sorbonne Paris Cité, 75270, Paris, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Dairou
mesh_terms: D000111:Acetylcysteine / Q000737:chemistry; D016477:Artifacts; D003470:Culture Media / Q000737:chemistry; D003545:Cysteine / Q000737:chemistry / Q000378:metabolism; D004926:Escherichia coli / Q000378:metabolism; D005947:Glucose / Q000737:chemistry; D017127:Glycation End Products, Advanced / Q000378:metabolism; D006031:Glycosylation; D006037:Glyoxal / Q000737:chemistry; D006801:Humans; D047908:Intracellular Signaling Peptides and Proteins / Q000378:metabolism; D009154:Mutation; D015513:Oncogene Proteins / Q000378:metabolism*; D020734:Parkinsonian Disorders / Q000378:metabolism; D000071617:Protein Deglycase DJ-1 / Q000378:metabolism*; D011765:Pyruvaldehyde / Q000378:metabolism
chemical_list: D003470:Culture Media; D017127:Glycation End Products, Advanced; D047908:Intracellular Signaling Peptides and Proteins; D015513:Oncogene Proteins; D006037:Glyoxal; D011765:Pyruvaldehyde; C105131:PARK7 protein, human; D000071617:Protein Deglycase DJ-1; D005947:Glucose; D003545:Cysteine; D000111:Acetylcysteine
keywords: Carbonyl stress; Electrophile stress; Glycation; Maillard reaction; Protein repair; Tris