title: Polymorphism of 41 kD Flagellin Gene and Its Human B-Cell Epitope inBorrelia burgdorferiStrains of China.
abstract: The 41 kD flagellin ofBorrelia burgdorferi (B. burgdorferi)is a major component of periplasmic flagellar filament core and a good candidate for serodiagnosis in early stage of Lyme disease. Here, we chose 89B. burgdorferistrains in China, amplified the gene encoding the 41 kD flagellin, and compared the sequences. The results showed that genetic diversity presented in the 41 kD flagellin genes of all 89 strains among the four genotypes ofB. burgdorferi, especially in the genotype ofB. garinii. Some specific mutation sites for each genotype of the 41 kD flagellin genes were found, which could be used for genotypingB. burgdorferistrains in China. Human B-cell epitope analysis showed that thirteen of 15 nonsynonymous mutations occurred in the epitope region of 41 kD flagellin and thirty of 42 B-cell epitopes were altered due to all 13 nonsynonymous mutations in the epitope region, which may affect the function of the antigen. Nonsynonymous mutations and changed human B-cell epitopes exist in 41 kD flagellin ofB. burgdorferi sensu latostrains; these changes should be considered in serodiagnosis of Lyme disease.
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
authors.forename: Huixin
authors.initials: H
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
authors.forename: Xuexia
authors.initials: X
authors.lastname: Hou
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhang
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
authors.forename: Qin
authors.identifier: 0000-0002-0849-2826
authors.initials: Q
authors.lastname: Hao
authors.affiliation: State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, China.
authors.forename: Kanglin
authors.identifier: 0000-0002-3997-0237
authors.initials: K
authors.lastname: Wan
mesh_terms: D025065:Borrelia burgdorferi / Q000235:genetics* / Q000276:immunology / Q000302:isolation & purification; D002681:China; D018985:Epitopes, B-Lymphocyte / Q000097:blood / Q000235:genetics / Q000276:immunology; D005408:Flagellin / Q000097:blood / Q000235:genetics*; D005838:Genotype; D006801:Humans; D008193:Lyme Disease / Q000097:blood* / Q000235:genetics / Q000276:immunology; D009154:Mutation; D011110:Polymorphism, Genetic; D012698:Serologic Tests
chemical_list: D018985:Epitopes, B-Lymphocyte; D005408:Flagellin