title: A novel fluorimetric sensing platform for highly sensitive detection of organophosphorus pesticides by using egg white-encapsulated gold nanoclusters.
abstract: Assays for organophosphorus pesticides (OPs) with high sensitivity as well as on-site screening have been urgently required to protect ecosystem and prevent disease. Herein, a novel fluorimetric sensing platform was constructed for quantitative detection of OPs via tyrosinase (TYR) enzyme-controlled quenching of gold nanoclusters (AuNCs). One-step green synthetic approach was developed for the synthesis of AuNCs by using chicken egg white (CEW) as template and stabilizer. Initially, TYR can catalyze the oxidation of dopamine to dopaminechrome, which can efficiently quench the fluorescence intensity of AuNCs at 630nm based on dynamic quenching process. However, with the presence of OPs, the activity of TYR was inhibited, resulting in the fluorescence recovery of AuNCs. This proposed fluorescence platform was demonstrated to enable rapid detection for OPs (paraoxon as model) and to provide excellent sensitivity with a detection limit of 0.1ngmL-1. Significantly, the fluorescence probe was used to prepare paper-based test strips for visual detection of OPs, which validated the excellent potential for real-time and on-site application.
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Xu
authors.initials: X
authors.lastname: Yan
authors.affiliation: School of Pharmacy, Jilin University, Changchun 130021, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Li
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Hu
authors.affiliation: Department of Analytical Chemistry, College of Chemistry, Jilin University, Changchun 130012, China. Electronic address: suxg@jlu.edu.cn.
authors.forename: Xingguang
authors.initials: X
authors.lastname: Su
mesh_terms: D000818:Animals; D015374:Biosensing Techniques / Q000295:instrumentation*; D002645:Chickens; D004529:Egg White / Q000737:chemistry*; D004867:Equipment Design; D005470:Fluorometry / Q000295:instrumentation; D006046:Gold / Q000737:chemistry*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D008958:Models, Molecular; D014442:Monophenol Monooxygenase / Q000037:antagonists & inhibitors / Q000378:metabolism; D010209:Paper; D010261:Paraoxon / Q000032:analysis* / Q000378:metabolism; D010575:Pesticides / Q000032:analysis* / Q000378:metabolism; D011934:Reagent Strips / Q000032:analysis*
chemical_list: D010575:Pesticides; D011934:Reagent Strips; D006046:Gold; D014442:Monophenol Monooxygenase; D010261:Paraoxon
keywords: Fluorescence; Gold nanoclusters; Organophosphorus pesticides; Test strips