title: Metabolomics with Nuclear Magnetic Resonance Spectroscopy in a Drosophila melanogaster Model of Surviving Sepsis.
abstract: Patients surviving sepsis demonstrate sustained inflammation, which has been associated with long-term complications. One of the main mechanisms behind sustained inflammation is a metabolic switch in parenchymal and immune cells, thus understanding metabolic alterations after sepsis may provide important insights to the pathophysiology of sepsis recovery. In this study, we explored metabolomics in a novelDrosophila melanogastermodel of surviving sepsis using Nuclear Magnetic Resonance (NMR), to determine metabolite profiles. We used a model of percutaneous infection inDrosophila melanogasterto mimic sepsis. We had three experimental groups: sepsis survivors (infected with Staphylococcus aureus and treated with oral linezolid), sham (pricked with an aseptic needle), and unmanipulated (positive control). We performed metabolic measurements seven days after sepsis. We then implemented metabolites detected in NMR spectra into the MetExplore web server in order to identify the metabolic pathway alterations in sepsis survivingDrosophila. Our NMR metabolomic approach in aDrosophilamodel of recovery from sepsis clearly distinguished between all three groups and showed two different metabolomic signatures of inflammation. Sham flies had decreased levels of maltose, alanine, and glutamine, while their level of choline was increased. Sepsis survivors had a metabolic signature characterized by decreased glucose, maltose, tyrosine, beta-alanine, acetate, glutamine, and succinate.
authors.affiliation: Clinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, 15261 PA, USA. bakalovv@upmc.edu.
authors.forename: Veli
authors.initials: V
authors.lastname: Bakalov
authors.affiliation: Laboratoire Chimie, Structures, Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), UMR 7244, Université Paris 13, 93000 Bobigny, France. roland.amathieu@aphp.fr.|Intensive Care Unit, Jean Verdier University Hospital, Paris 13 University, 93140 Bondy, France. roland.amathieu@aphp.fr.
authors.forename: Roland
authors.initials: R
authors.lastname: Amathieu
authors.affiliation: Laboratoire Chimie, Structures, Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), UMR 7244, Université Paris 13, 93000 Bobigny, France. mohamed.triba@univ-paris13.fr.
authors.forename: Mohamed N
authors.initials: MN
authors.lastname: Triba
authors.affiliation: Laboratoire Structure-Activité des biomolécules Normales et Pathologiques (SABNP), U1204, Université d'Evry Val d'Essonne, 91025 Evry, France. mariejeanne.laicheong@univ-evry.fr.
authors.forename: Marie-Jeanne
authors.initials: MJ
authors.lastname: Clément
authors.affiliation: Clinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, 15261 PA, USA. laurare3@gmail.com.
authors.forename: Laura
authors.initials: L
authors.lastname: Reyes Uribe
authors.affiliation: Unité de Biologie Intégrative des Adaptations à l'Exercice (UBIAE), EA 7362, Université d'Evry Val d'Essonne, 91025 Evry, France. laurence.lemoyec@univ-evry.fr.
authors.forename: Laurence
authors.initials: L
authors.lastname: Le Moyec
authors.affiliation: Clinical Research, Investigation, and Systems Modeling of Acute Illness (CRISMA) Laboratory, Department of Critical Care Medicine, University of Pittsburgh School of Medicine, Pittsburgh, 15261 PA, USA. kaynarm@upmc.edu.|Department of Anesthesiology, University of Pittsburgh, Pittsburgh, 15261 PA, USA. kaynarm@upmc.edu.
authors.forename: Ata Murat
authors.initials: AM
authors.lastname: Kaynar
keywords: NMR; metabolomics; sepsis; survival