title: Single Low-Dose Ionizing Radiation Induces Genotoxicity in Adult Zebrafish and its Non-Irradiated Progeny.
abstract: This study investigated to what extent a single exposure to low doses of ionizing radiation can induce genotoxic damage in irradiated adult zebrafish (Danio rerio) and its non-irradiated F1 progeny. Four groups of adult zebrafish were irradiated with a single dose of X-rays at 0 (control), 100, 500 and 1000 mGy, respectively, and couples of each group were allowed to reproduce following irradiation. Blood of parental fish and whole-body offspring were analysed by the comet assay for detection of DNA damage. The level of DNA damage in irradiated parental fish increased in a radiation dose-dependent manner at day 1 post-irradiation, but returned to the control level thereafter. The level of DNA damage in the progeny was directly correlated with the parental irradiation dose. Results highlight the genotoxic risk of a single exposure to low-dose ionizing radiation in irradiated individuals and also in its non-irradiated progeny.
authors.affiliation: ICBAS - Institute of Biomedical Sciences, University of Porto, Rua de Jorge Viterbo Ferreira 228, 4050-313, Porto, Portugal.|Nuclear Medicine Department, High Institute for Allied Health Technologies of Porto - Polytechnic Institute of Porto (ESTSP.IPP), Rua Valente Perfeito 322, 4400-330, Vila Nova de Gaia, Portugal.
authors.forename: J
authors.initials: J
authors.lastname: Lemos
authors.affiliation: CIIMAR/CIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal.
authors.forename: T
authors.initials: T
authors.lastname: Neuparth
authors.affiliation: Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal.
authors.forename: M
authors.initials: M
authors.lastname: Trigo
authors.affiliation: Nuclear Medicine Department, High Institute for Allied Health Technologies of Porto - Polytechnic Institute of Porto (ESTSP.IPP), Rua Valente Perfeito 322, 4400-330, Vila Nova de Gaia, Portugal.
authors.forename: P
authors.initials: P
authors.lastname: Costa
authors.affiliation: Nuclear Medicine Department, High Institute for Allied Health Technologies of Porto - Polytechnic Institute of Porto (ESTSP.IPP), Rua Valente Perfeito 322, 4400-330, Vila Nova de Gaia, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Vieira
authors.affiliation: IsoPor SA, PO box 4028, 4445, Ermesinde, Portugal.
authors.forename: L
authors.initials: L
authors.lastname: Cunha
authors.affiliation: Radiotherapy Deptartment, Júlio Teixeira SA, Rua Arquitecto Cassiano Barbosa 6, F, Sala 26, 4100-009, Porto, Portugal.
authors.forename: F
authors.initials: F
authors.lastname: Ponte
authors.affiliation: Radiotherapy Deptartment, Júlio Teixeira SA, Rua Arquitecto Cassiano Barbosa 6, F, Sala 26, 4100-009, Porto, Portugal.
authors.forename: P S
authors.initials: PS
authors.lastname: Costa
authors.affiliation: Nuclear Medicine Department, High Institute for Allied Health Technologies of Porto - Polytechnic Institute of Porto (ESTSP.IPP), Rua Valente Perfeito 322, 4400-330, Vila Nova de Gaia, Portugal.|IsoPor SA, PO box 4028, 4445, Ermesinde, Portugal.
authors.forename: L F
authors.initials: LF
authors.lastname: Metello
authors.affiliation: CIIMAR/CIMAR - Interdisciplinary Centre of Marine and Environmental Research, University of Porto, Rua dos Bragas 289, 4050-123, Porto, Portugal. apcarval@fc.up.pt.|Department of Biology, Faculty of Sciences, University of Porto, Rua do Campo Alegre, 4169-007, Porto, Portugal. apcarval@fc.up.pt.
authors.forename: A P
authors.initials: AP
authors.lastname: Carvalho
mesh_terms: D000818:Animals; D020552:Comet Assay; D004249:DNA Damage; D004307:Dose-Response Relationship, Radiation; D005720:Gamma Rays; D000069079:Radiation Exposure; D011839:Radiation, Ionizing; D012098:Reproduction; D015027:Zebrafish / Q000235:genetics*
keywords: Comet assay; Genotoxicity; Ionizing radiation; Zebrafish