title: UV-irradiated parylene surfaces for proliferation and differentiation of PC-12 cells.
abstract: PC-12 cells originate from neuroblastic cells, which have an ability to differentiate into neuronlike cells. In this work, the purpose was to estimate the influence of microenvironments on cell attachment and neuritogenesis capacity of PC-12 cells on parylene-N and parylene-C films with and without ultraviolet (UV) light treatment. The estimate of total cell number after incubation for 72h, the ratio of adherent to suspended cells, counting of neurite outgrowths on parylene-N or parylene-C films after UV exposure suggested that these films were suitable for proliferation as well as differentiation of PC-12 cells. The differences in surface properties of parylene-N and parylene-C films with and without UV exposure were analyzed by contact angle measurement, Fourier-transform infrared spectrometry, and X-ray photoelectron spectroscopy. According to these analyses, introduction of oxygen-related chemical functional groups was presumed to result in increased hydrophilicity and efficiency of protein immobilization on parylene-N and parylene-C films after UV treatment. According to fluorescent staining, western blotting, and cell cycle analysis, UV-treated parylene-C and parylene-N films appear to effectively facilitate simultaneous proliferation and differentiation of PC-12 cells with neurite outgrowth.
authors.affiliation: Graduate Program of Nano Science and Technology, Yonsei University, 50-Yonsei Ro, Seodaemun-gu, Seoul, 120-749, South Korea.
authors.forename: Usman
authors.initials: U
authors.lastname: Liaqat
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50-Yonsei Ro, Seodaemun-gu, Seoul, 120-749, South Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Ko
authors.affiliation: Department of Medical Engineering, Yonsei University, College of Medicine, 50-Yonsei Ro, Seodaemun-gu, Seoul, 120-749, South Korea.
authors.forename: Hwal
authors.initials: H
authors.lastname: Suh
authors.affiliation: College of Life Science and Bioengineering, Incheon National University, Incheon, 406-772, South Korea.
authors.forename: Misu
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Materials Science and Engineering, Yonsei University, 50-Yonsei Ro, Seodaemun-gu, Seoul, 120-749, South Korea. Electronic address: jcpyun@yonsei.ac.kr.
authors.forename: Jae-Chul
authors.initials: JC
authors.lastname: Pyun
mesh_terms: D000818:Animals; D001709:Biotechnology; D002448:Cell Adhesion; D018929:Cell Culture Techniques; D002454:Cell Differentiation; D049109:Cell Proliferation; D060833:Cellular Microenvironment; D016501:Neurites / Q000648:ultrastructure; D009474:Neurons / Q000166:cytology* / Q000502:physiology; D016716:PC12 Cells; D011108:Polymers / Q000528:radiation effects; D051381:Rats; D013499:Surface Properties; D014466:Ultraviolet Rays; D014992:Xylenes / Q000528:radiation effects
chemical_list: D011108:Polymers; D014992:Xylenes; C011055:parylene
keywords: Differentiation; Neurite outgrowth; PC-12; Parylene; Proliferation; UV-treatment