title: Effects of Electroacupuncture on the Daily Rhythmicity of Intestinal Movement and Circadian Rhythmicity of ColonicPer2Expression in Rats with Spinal Cord Injury.
abstract: Background. Spinal cord injury (SCI) leads to bowel dysfunction. Electroacupuncture (EA) may improve bowel function.Objective. To assess EA on daily rhythmicity of intestinal movement and circadian rhythmicity of colonicPer2expression in rats with SCI.Methods. Rats were randomized to the sham, SCI, and SCI+EA groups. EA was performed at bilateral Zusanli point (ST36) during daytime (11:00-11:30) for 14 days following SCI. Intestinal transit and daily rhythmicity of intestinal movement were assessed. Circadian rhythmicity of colonicPer2expression was assessed by real-time RT-PCR.Results. EA shortened the stool efflux time and increased the dry fecal weight within 24 h in SCI rats. Daily rhythmicity of intestinal movements was unaffected by SCI. The expression of colonicPer2peaked at 20:00 and the nadir was observed at 8:00 in the SCI and sham groups. In the SCI+EA group, colonicPer2expression peaked at 12:00 and 20:00, and the nadir was observed at 8:00.Conclusion. SCI did not change the circadian rhythmicity of colonicPer2expression in rats, and daily intestinal movement rhythmicity was retained. EA changed the daily rhythmicity of intestinal movement and the circadian rhythmicity of colonicPer2expression in rats with SCI, increasingPer2expression shortly after EA treatment.
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Jie
authors.identifier: 0000-0002-7568-8669
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Department of Sport Rehabilitation, Shanghai University of Sport, Shanghai 200438, China.
authors.forename: Xueqiang
authors.initials: X
authors.lastname: Wang
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Jiabao
authors.identifier: 0000-0001-8398-2369
authors.initials: J
authors.lastname: Guo
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Yujie
authors.identifier: 0000-0002-7202-3776
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Rehabilitation Medicine Center, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhang
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Xie
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Zhaojin
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China.
authors.forename: Yuemei
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Nanjing University of Chinese Medicine, Nanjing 210023, China; Rehabilitation Center, Hainan Provincial Nongken General Hospital, Haikou 570311, China.
authors.forename: Yi
authors.identifier: 0000-0002-7673-7709
authors.initials: Y
authors.lastname: Zhu
mesh_terms: D000818:Animals; D002940:Circadian Rhythm; D003106:Colon / Q000378:metabolism / Q000503:physiopathology; D015671:Electroacupuncture; D005260:Female; D005769:Gastrointestinal Motility; D005786:Gene Expression Regulation; D008297:Male; D056950:Period Circadian Proteins / Q000096:biosynthesis*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013119:Spinal Cord Injuries / Q000378:metabolism / Q000503:physiopathology / Q000628:therapy
chemical_list: C513311:Per2 protein, rat; D056950:Period Circadian Proteins