title: Differences in levels of platelet-derived microparticles in platelet components prepared using the platelet rich plasma, buffy coat, and apheresis procedures.
abstract: BACKGROUND
  There has been an increased interest in platelet-derived microparticles (PMPs) in transfusion medicine. Little is known about PMP status during the preparation of platelet concentrates for transfusion.
  
  
  OBJECTIVE
  The aim of this study is to compare the PMP levels in platelet components prepared using the buffy coat (BC), platelet-rich plasma platelet concentrate (PRP-PC), and apheresis (AP) processes.
  
  
  METHODS
  Platelet components were prepared using the PRP-PC and BC processes. Apheresis platelets were prepared using the Trima Accel and Amicus instruments. The samples were incubated with annexin A5-FITC, CD41-PE, and CD62P-APC. At day 1 after processing, the PMPs and activated platelets were determined using flow cytometry.
  
  
  RESULTS
  Both the percentage and number of PMPs were higher in platelet components prepared using the Amicus instrument (2.6±1.8, 32802±19036 particles/μL) than in platelet components prepared using the Trima Accel instrument (0.5±0.4, 7568±5298 particles/μL), BC (1.2±0.6, 12,920±6426 particles/μL), and PRP-PC (0.9±0.6, 10731±5514 particles/μL). Both the percentage and number of activated platelets were higher in platelet components prepared using the Amicus instrument (33.2±13.9, 427553±196965 cells/μL) than in platelet components prepared using the Trima Accel instrument (16.2±6.1, 211209±87706 cells/μL), BC (12.9±3.2, 140624±41003 cells/μL), and PRP-PC (21.1±6.3, 265210±86257 cells/μL).
  
  
  CONCLUSIONS
  The study suggests high variability of PMPs and activated platelets in platelet components prepared using different processes. This result may be important in validating the instruments involved in platelet blood collection and processing.
authors.affiliation: Research Division, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address: egarit.nou@mahidol.ac.th.
authors.forename: Egarit
authors.initials: E
authors.lastname: Noulsri
authors.affiliation: Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Prapaporn
authors.initials: P
authors.lastname: Udomwinijsilp
authors.affiliation: Department of Research and Development, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Surada
authors.initials: S
authors.lastname: Lerdwana
authors.affiliation: Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand.
authors.forename: Viroje
authors.initials: V
authors.lastname: Chongkolwatana
authors.affiliation: Department of Transfusion Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand. Electronic address: parichart.per@mahidol.ac.th.
authors.forename: Parichart
authors.initials: P
authors.lastname: Permpikul
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D058621:Blood Buffy Coat / Q000378:metabolism; D001781:Blood Component Removal / Q000379:methods*; D001792:Blood Platelets; D055252:Cell-Derived Microparticles; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D053657:Platelet-Rich Plasma
keywords: Apheresis; Buffy coat; Platelet component; Platelet rich plasma; Platelet-derived microparticle