title: Efficient targeted mutagenesis in Epichloë festucae using a split marker system.
abstract: A split-marker system for targeted gene deletion was developed for the model grass endophytic fungus Epichloë festucae. Compared to the conventional system that yields up to 25% homologous recombinants, the method resulted in 33-74% targeted deletions in E. festucae using as little as 1.5kb of targeting sequence.
authors.affiliation: AgResearch, Grasslands Research Centre, Palmerston North, New Zealand; School of Biological Sciences, University of Auckland, New Zealand.
authors.forename: M
authors.initials: M
authors.lastname: Rahnama
authors.affiliation: AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: N
authors.initials: N
authors.lastname: Forester
authors.affiliation: AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: K G S U
authors.initials: KG
authors.lastname: Ariyawansa
authors.affiliation: AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: C R
authors.initials: CR
authors.lastname: Voisey
authors.affiliation: AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: L J
authors.initials: LJ
authors.lastname: Johnson
authors.affiliation: AgResearch, Grasslands Research Centre, Palmerston North, New Zealand.
authors.forename: R D
authors.initials: RD
authors.lastname: Johnson
authors.affiliation: AgResearch, Grasslands Research Centre, Palmerston North, New Zealand; Biotelliga Ltd., Auckland, New Zealand. Electronic address: damien@biotelliga.com.
authors.forename: D J
authors.initials: DJ
authors.lastname: Fleetwood
mesh_terms: D003001:Cloning, Molecular / Q000379:methods*; D055329:Epichloe / Q000235:genetics*; D005656:Fungal Proteins / Q000235:genetics*; D017353:Gene Deletion; D005822:Genetic Vectors; D016296:Mutagenesis; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D011995:Recombination, Genetic
chemical_list: D005656:Fungal Proteins
keywords: Endophyte; Epichloë; Functional analysis; Gene deletion; Split-marker