title: Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells.
abstract: Mesenchymal stem cells (MSCs) are adult stem cells with multilineage potential, which makes them attractive tools for regenerative medicine applications. Efficient gene transfer into MSCs is essential not only for basic research in developmental biology but also for therapeutic applications involving gene-modification in regenerative medicine. Adenovirus vectors (Advs) can efficiently and transiently introduce an exogenous gene into many cell types via their primary receptors, the coxsackievirus and adenovirus receptors, but not into MSCs, which are deficient in coxsackievirus and adenovirus receptors expression. To overcome this problem, we developed an Adv coated with a spermine-pullulan (SP) cationic polymer and investigated its physicochemical properties and internalization mechanisms. We demonstrated that the SP coating could enhance adenoviral transduction of MSCs without detectable cytotoxicity or effects on differentiation. Our results argue in favor of the potentiality of the SP-coated Adv as a prototype vector for efficient and safe transduction of MSCs.
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing.
authors.forename: Li
authors.initials: L
authors.lastname: Wan
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing; Institute of Pharmaceutics, Zhejiang University, Hangzhou; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences.
authors.forename: Xinglei
authors.initials: X
authors.lastname: Yao
authors.affiliation: State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences.
authors.forename: Francesco
authors.initials: F
authors.lastname: Faiola
authors.affiliation: YouAn Hospital, Capital Medical University, Beijing, People's Republic of China.
authors.forename: Bojun
authors.initials: B
authors.lastname: Liu
authors.affiliation: Institute of Pharmaceutics, Zhejiang University, Hangzhou.
authors.forename: Tianyuan
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Biomaterials, Field of Tissue Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto.
authors.forename: Yasuhiko
authors.initials: Y
authors.lastname: Tabata
authors.affiliation: Department of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences, Osaka University, Osaka.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.affiliation: Department of Biotechnology and Therapeutics, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka, Japan.
authors.forename: Shinsaku
authors.initials: S
authors.lastname: Nakagawa
authors.affiliation: Institute of Pharmaceutics, Zhejiang University, Hangzhou.
authors.forename: Jian-Qing
authors.initials: JQ
authors.lastname: Gao
authors.affiliation: Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine Peking Union Medical College, Peking Union Medical College Hospital, Center of Excellence in Tissue Engineering Chinese Academy of Medical Sciences, Beijing.
authors.forename: Robert Chunhua
authors.initials: RC
authors.lastname: Zhao
mesh_terms: D000256:Adenoviridae / Q000235:genetics*; D000818:Animals; D002412:Cations; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D005822:Genetic Vectors / Q000737:chemistry*; D005936:Glucans / Q000737:chemistry*; D006801:Humans; D008156:Luciferases / Q000235:genetics; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D011108:Polymers / Q000737:chemistry / Q000494:pharmacology; D017207:Rats, Sprague-Dawley; D013096:Spermine / Q000737:chemistry / Q000494:pharmacology*; D014161:Transduction, Genetic / Q000379:methods*; D019076:Transgenes
chemical_list: D002412:Cations; D005936:Glucans; D011108:Polymers; D013096:Spermine; C009109:pullulan; D008156:Luciferases
keywords: adenovirus vectors; gene transduction; mesenchymal stem cells; polymer; spermine-pullulan