title: Ethylene glycol, but not DMSO, could replace glycerol inclusion in soybean lecithin-based extenders in ram sperm cryopreservation.
abstract: The aim of this study was to evaluate the effects of glycerol, ethylene glycol or DMSO in a soybean lecithin extender for freezing ram semen. In this study, 20 ejaculates were collected from four Ghezel rams and diluted with soybean lecithin extender with glycerol (7%), ethylene glycol (3%, 5% and 7%) or DMSO (3%, 5% and 7%). Sperm motility (CASA), membrane integrity (HOS test), viability, total abnormality, mitochondrial activity (Rhodamine 123) and apoptotic features (Annexin V/Propidium iodide) were assessed after thawing. There was no significant difference between glycerol and ethylene glycol at different concentrations (3% and 5%) regarding sperm total and progressive motility, viability, and membrane integrity. The least percentages of mitochondrial functionality were observed in samples frozen with all different DMSO concentrations tested (P<0.05). Moreover, the percentage of post-thawed dead sperm was the greatest for all the DMSO concentrations compared with other groups (P<0.05). Thus, DMSO had an adverse effect on the post thaw ram sperm parameters. In contrast, ethylene glycol could be a desirable substitute of glycerol in the freezing extender, in view of similar results obtained in post-thaw quality of ram semen cryopreserved in a soybean lecithin extender. We propose that glycerol in a soybean lecithin based extender could be replaced by ethylene glycol at 3% or 5% concentrations.
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
authors.forename: Abouzar
authors.initials: A
authors.lastname: Najafi
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran. Electronic address: daghighkia@tabrizu.ac.ir.
authors.forename: Hossein
authors.initials: H
authors.lastname: Daghigh-Kia
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
authors.forename: Hossein Vaseghi
authors.initials: HV
authors.lastname: Dodaran
authors.affiliation: Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran.
authors.forename: Mahdieh
authors.initials: M
authors.lastname: Mehdipour
authors.affiliation: Department of Clinical and Experimental Medicine (IKE), Division of Clinical Sciences, Obstetrics and Gynecology, Linköping University, Linköping, Sweden.
authors.forename: Manuel
authors.initials: M
authors.lastname: Alvarez-Rodriguez
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000379:methods* / Q000662:veterinary; D003451:Cryoprotective Agents / Q000494:pharmacology*; D004121:Dimethyl Sulfoxide / Q000494:pharmacology; D019855:Ethylene Glycol / Q000494:pharmacology*; D005990:Glycerol / Q000494:pharmacology*; D054709:Lecithins / Q000494:pharmacology*; D008297:Male; D037121:Plant Lectins / Q000494:pharmacology; D012661:Semen / Q000187:drug effects; D012662:Semen Preservation / Q000379:methods* / Q000662:veterinary; D012756:Sheep; D030262:Soybean Proteins / Q000494:pharmacology; D013081:Sperm Motility / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D054709:Lecithins; D037121:Plant Lectins; D030262:Soybean Proteins; D019855:Ethylene Glycol; D005990:Glycerol; D004121:Dimethyl Sulfoxide
keywords: Cryopreservation; DMSO; Ethylene glycol; Glycerol; Ram sperm; Soybean lecithin