title: Nanoscale Proteinosomes Fabricated by Self-Assembly of a Supramolecular Protein-Polymer Conjugate.
abstract: Proteinosomes are a type of protein-based spherical capsules, which have potential applications in drug delivery, cell imaging, gene expression, and biocatalysis. In this research, a novel approach to the fabrication of proteinosomes entirely composed of protein molecules based on self-assembly of a supramolecular protein-polymer conjugate is proposed. A supramolecular protein-polymer conjugate was prepared by mixing βCD-modified bovine serum albumin (BSA) and adamantane-terminated poly(N-isopropylamide) (Ad-PNIPAM) in aqueous solution. The BSA-PNIPAM bioconjugate self-assembled into micelles with PNIPAM cores and BSA coronae at a temperature above the lower critical solution temperature (LCST) of PNIPAM. After cross-linking of BSA in the coronae, and followed by addition of excess βCD, PNIPAM chains were cleaved from the micellar structures, and nanoscale proteinosomes were prepared. The dual-responsive proteinosomes dissociated in the presence of trypsin or glutathione.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
authors.forename: Guangda
authors.initials: G
authors.lastname: Han
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
authors.forename: Jin-Tao
authors.initials: JT
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
authors.forename: Xiaotian
authors.initials: X
authors.lastname: Ji
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
authors.forename: Li
authors.initials: L
authors.lastname: Liu
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Nankai University; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin) , Tianjin 300071, China.
authors.forename: Hanying
authors.identifier: 0000-0002-0706-9188
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000218:Adamantane / Q000031:analogs & derivatives*; D000818:Animals; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry*; D008823:Micelles; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D047392:beta-Cyclodextrins / Q000737:chemistry*
chemical_list: D000180:Acrylic Resins; D004337:Drug Carriers; D008823:Micelles; D047392:beta-Cyclodextrins; C052970:poly-N-isopropylacrylamide; D012710:Serum Albumin, Bovine; D000218:Adamantane