title: Phyllanthus emblica seed extract mediated synthesis of PdNPs against antibacterial, heamolytic and cytotoxic studies.
abstract: Ecofriendly synthesis of Palladium nanoparticles (PdNPs) were achieved using Phyllanthus emblica (P. emblica) seeds as reducing agent. Further the ecofriendly synthesized PdNPs were subjected for various analytical techniques like UV-Vis, FT-IR, XRD, Zeta potential, SEM and TEM. The results indicated that green synthesized PdNPs were spherical in shape with average particle size of 28±2nm with moderate stability. Further the synthesized PdNPs and extract were subjected for its antibacterial studies against various disease causing pathogens by agar well diffusion method. Seed extract resulted in 8.9±1.46mm against B. subtilis and PdNPs showed 9.6±1.10mm against S. aureus and synthesized PdNPs and extract were tested for hemolytic which resulted in 20% and 10% respectively. Toxicity studies were done against Artemia salina (A. salina). The LC50value of green synthesized P. emblica capped PdNPs and the P. emblica seed extract were found to be less toxic for A. salina with a value of 1.00μg/mL and 1.25μg/mL. In addition samples were checked for in vitro cytotoxicity assays on HeLa cell lines.
authors.affiliation: Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632 014, Tamilnadu, India.
authors.forename: Murugesan
authors.initials: M
authors.lastname: Dinesh
authors.affiliation: Chemistry of Heterocycles & Natural Product Research Laboratory, Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632 014, Tamilnadu, India. Electronic address: mohanaroopan.s@vit.ac.in.
authors.forename: Selvaraj Mohana
authors.initials: SM
authors.lastname: Roopan
authors.affiliation: Department of Biotechnology, School of Biosciences and Technology, VIT University, Vellore 632 014, Tamilnadu, India. Electronic address: immanuelselvaraj@vit.ac.in.
authors.forename: Chinnadurai Immanuel
authors.initials: CI
authors.lastname: Selvaraj
authors.affiliation: Electrochemistry Research Group, Chemistry Department, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Prabhakarn
authors.initials: P
authors.lastname: Arunachalam
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002470:Cell Survival / Q000187:drug effects*; D006461:Hemolysis / Q000187:drug effects*; D006801:Humans; D053768:Metal Nanoparticles; D008826:Microbial Sensitivity Tests; D008854:Microscopy, Electron / Q000379:methods; D010165:Palladium / Q000737:chemistry / Q000494:pharmacology*; D029842:Phyllanthus emblica / Q000737:chemistry*; D010936:Plant Extracts / Q000494:pharmacology*; D012639:Seeds / Q000737:chemistry*; D013057:Spectrum Analysis; D014961:X-Ray Diffraction
chemical_list: D000900:Anti-Bacterial Agents; D010936:Plant Extracts; D010165:Palladium
keywords: Artemia salina; Haemolysis; HeLa cell lines; PdNPs; Phyllanthus emblica