title: The overexpressed functional transient receptor potential channel TRPM2 in oral squamous cell carcinoma.
abstract: TRPM2, one member of the transient receptor potential (TRP) protein super-family, is a Ca2+-permeable channel that is activated by oxidative stress and confers susceptibility to cell death. In the human tongue specimens of carcinoma and the tongue carcinoma SCC cell lines, we observed the enhanced expression of TRPM2. By means of the whole-cell electrophysiological recording, the ADPR-induced currents mediated by TRPM2 were recorded in cultured SCC9 cells. Moreover, after H2O2treatment for 24 hours, the apoptotic number of SCC9 cells was significantly increased. However, the selectively knocked-down TRPM2 with the small interfering RNA technique inhibited the survival and migration of the SCC9 cancer cells, which was independent of the p53-p21 pathway, since the expression of p21 was enhanced after TRPM2 knockdown. Furthermore, the sub-cellular localization of TRPM2 was remarkably different between cancerous and non-cancerous cells. A significant amount of the TRPM2 proteins were located in the nuclei in cancer cells. All these data suggest that TRPM2 is essential for the survival and migration of SCC cancer cells and may be a potential target for the selective treatment of tongue cancer.
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Ling-Yan
authors.initials: LY
authors.lastname: Zhao
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.|Shanghai Key Laboratory of Stomatology and Shanghai Research Institute of Stomatology, Department of Oral and Maxillofacial-Head and Neck Oncology, Ninth People's Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, 200011, China.
authors.forename: Wan-Lin
authors.initials: WL
authors.lastname: Xu
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Zeng-Qi
authors.initials: ZQ
authors.lastname: Xu
authors.affiliation: Key Laboratory of Human Functional Genomics of Jiangsu, Department of Neurobiology, Nanjing Medical University, 101 Longmian Road, Nanjing, Jiangsu, 211166, China.
authors.forename: Cui
authors.initials: C
authors.lastname: Qi
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Cheng
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Lai-Kui
authors.initials: LK
authors.lastname: Liu
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Yu-Nong
authors.initials: YN
authors.lastname: Wu
authors.affiliation: Key Laboratory of Human Functional Genomics of Jiangsu, Department of Neurobiology, Nanjing Medical University, 101 Longmian Road, Nanjing, Jiangsu, 211166, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.affiliation: Jiangsu Key Laboratory of Oral Diseases and Department of Oral and maxillofacial surgery, Affiliated Hospital of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, China.
authors.forename: Jin-Hai
authors.initials: JH
authors.lastname: Ye
mesh_terms: D000246:Adenosine Diphosphate Ribose / Q000494:pharmacology; D002294:Carcinoma, Squamous Cell / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects; D002467:Cell Nucleus / Q000187:drug effects / Q000378:metabolism; D051152:Clusterin / Q000378:metabolism*; D015536:Down-Regulation / Q000187:drug effects / Q000235:genetics; D017353:Gene Deletion; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology; D015640:Ion Channel Gating / Q000187:drug effects; D009062:Mouth Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D013347:Subcellular Fractions / Q000378:metabolism; D014062:Tongue Neoplasms / Q000378:metabolism / Q000473:pathology; D015854:Up-Regulation / Q000187:drug effects / Q000235:genetics
chemical_list: D051152:Clusterin; D000246:Adenosine Diphosphate Ribose; D006861:Hydrogen Peroxide