title: Identification of an IRF-1 splicing transcript in APL cells sharing similar transactivation activity of the full length one.
abstract: Interferon regulatory factor-1 (IRF-1) is a member of the interferon regulatory factor family. It acts as a transcriptional activator and plays a critical role in antiviral defense, immune response, cell growth regulation, apoptosis and cell differentiation. Deletions, mutations or aberrant splicing of IRF-1 would result in its functional inactivation, and closely related to the tumorigenesis. In this work, we identified an IRF-1 splicing transcript (IRF-1-s) in all-trans retinoic acid (ATRA)-treated acute promyelocytic leukemia (APL) cell line NB4 cells. It lost the exon 8 and 9 of the full length IRF-1, expressed in numerous cell types and could be induced to expression by ATRA in NB4 cells. It turned out similar biological activity as full length IRF-1 to enhance the transcription of interferon stimulated response element (ISRE)-containing target genes. Identification of IRF-1-s in NB4 cells would be benefit for our further exploring the signaling pathway of ATRA and interferons, as well as the mechanisms of differentiation of APL cells.
authors.affiliation: Key Laboratory of Hematologic Malignancies of Zhejiang Province, Department of Hematology, Institute of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, People's Republic of China.
authors.forename: Yejiang
authors.initials: Y
authors.lastname: Lou
authors.affiliation: State Key Laboratory of Medical Genomics, Faculty of Medical Laboratory Science, Shanghai Institute of Hematology, Rui-jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
authors.forename: Di
authors.initials: D
authors.lastname: Xia
authors.affiliation: Key Laboratory of Hematologic Malignancies of Zhejiang Province, Department of Hematology, Institute of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, People's Republic of China.
authors.forename: Mengxia
authors.initials: M
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Medical Genomics, Faculty of Medical Laboratory Science, Shanghai Institute of Hematology, Rui-jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, People's Republic of China.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Tong
authors.affiliation: Key Laboratory of Hematologic Malignancies of Zhejiang Province, Department of Hematology, Institute of Hematology, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, Zhejiang, People's Republic of China. Electronic address: Jiej0503@163.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Jin
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D019556:COS Cells; D002454:Cell Differentiation; D045744:Cell Line, Tumor; D002522:Chlorocebus aethiops; D005091:Exons; D057809:HEK293 Cells; D006801:Humans; D050856:Interferon Regulatory Factor-1 / Q000235:genetics* / Q000378:metabolism; D008214:Lymphocytes / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D012326:RNA Splicing; D012333:RNA, Messenger / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment; D015398:Signal Transduction; D015533:Transcriptional Activation; D014212:Tretinoin / Q000494:pharmacology
chemical_list: C494257:IRF1 protein, human; D050856:Interferon Regulatory Factor-1; D012333:RNA, Messenger; D014212:Tretinoin
keywords: Acute promyelocytic leukemia; All-trans retinoic acid; IRF-1; Interferon; Splicing transcript