title: Identification of NAD+ capped mRNAs in Saccharomyces cerevisiae.
abstract: RNAs besides tRNA and rRNA contain chemical modifications, including the recently described 5' nicotinamide-adenine dinucleotide (NAD+) RNA in bacteria. Whether 5' NAD-RNA exists in eukaryotes remains unknown. We demonstrate that 5' NAD-RNA is found on subsets of nuclear and mitochondrial encoded mRNAs in Saccharomyces cerevisiae NAD-mRNA appears to be produced cotranscriptionally because NAD-RNA is also found on pre-mRNAs, and only on mitochondrial transcripts that are not 5' end processed. These results define an additional 5' RNA cap structure in eukaryotes and raise the possibility that this 5' NAD+cap could modulate RNA stability and translation on specific subclasses of mRNAs.
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO 80303.
authors.forename: Robert W
authors.initials: RW
authors.lastname: Walters
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO 80303.
authors.forename: Tyler
authors.initials: T
authors.lastname: Matheny
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO 80303.|Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303.
authors.forename: Laura S
authors.initials: LS
authors.lastname: Mizoue
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO 80303.|Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303.
authors.forename: Bhalchandra S
authors.initials: BS
authors.lastname: Rao
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO 80303.|Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303.
authors.forename: Denise
authors.initials: D
authors.lastname: Muhlrad
authors.affiliation: Department of Chemistry and Biochemistry, University of Colorado Boulder, Boulder, CO 80303; roy.parker@colorado.edu.|Howard Hughes Medical Institute, University of Colorado Boulder, Boulder, CO 80303.
authors.forename: Roy
authors.initials: R
authors.lastname: Parker
mesh_terms: D009243:NAD / Q000378:metabolism; D012315:RNA Caps / Q000235:genetics / Q000378:metabolism*; D012322:RNA Precursors / Q000235:genetics / Q000378:metabolism; D020871:RNA Stability; D012331:RNA, Fungal / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000077278:RNA, Mitochondrial; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic
chemical_list: D012315:RNA Caps; D012322:RNA Precursors; D012331:RNA, Fungal; D012333:RNA, Messenger; D000077278:RNA, Mitochondrial; C029305:mitochondrial messenger RNA; D009243:NAD
keywords: NAD-RNA; RNA modification; mitochondria; transcription