title: Supplementation with copper edetate in control of Haemonchus contortus of sheep, and its effect on cholinesterase's and superoxide dismutase activities.
abstract: The aim of this study was to evaluate the effect of copper edetate on biochemical parameters, oxidative profile, cholinesterase's activities, as well as its capacity to control gastrointestinal parasites in infected sheep. Thus, Lacaune sheep (n = 18) infected by Haemonchus contortus were used and divided into three groups of six animal each: the group A was composed of untreated animals (the control group), the group B was formed by animals treated with 0.3 mg/kg of copper edetate, and the group C was composed of animals treated with 0.5 mg/kg of copper edetate. Blood collection was performed on days 0, 10, 20 and 30 after mineral supplementation and different variables were measured. Cholinergic system was evaluated to determine the acetylcholinesterase (AChE) activity in total blood and butyrylcholinesterase (BChE) activity in serum. Eggs per gram of feces (EPG) were evaluated. There were no significant differences (P > 0.05) between groups regarding total protein, albumin, globulin and urea levels, GGT activity, as well as the hematocrit, and EPG. ALT activity decreased (P < 0.05) on groups B and C on day 30 compared to the control group (the group A). AChE activity decreased (P < 0.05) in the group C on days 10 and 30 compared to the control group, such decrease (P < 0.05) was also observed for BChE activity in the group C on day 10. ROS levels increased in the group C compared to groups A and B on day 10, while the SOD activity increased in the group C on days 20 and 30 compared to the control group (P < 0.05). CAT activity did not differ between groups (P > 0.05). In summary, the copper edetate was not efficient to control gastrointestinal parasites, but efficiently activated SOD, an important antioxidant enzyme. In addition, copper edetate was able to partially inhibit cholinesterase's activities when supplementated at its highest dose.
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Hyolanda M
authors.initials: HM
authors.lastname: Grosskopf
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil.
authors.forename: Rhayana K
authors.initials: RK
authors.lastname: Grosskopf
authors.affiliation: Graduate Program in Animal Science, UDESC, Chapecó, SC, Brazil.
authors.forename: Angelisa H
authors.initials: AH
authors.lastname: Biazus
authors.affiliation: Department of Large Animals, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Marta L R
authors.initials: ML
authors.lastname: Leal
authors.affiliation: Department of Large Animals, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Nathieli B
authors.initials: NB
authors.lastname: Bottari
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Mariana S
authors.initials: MS
authors.lastname: Alves
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Maria Rosa C
authors.initials: MR
authors.lastname: Schetinger
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil.
authors.forename: Vera M
authors.initials: VM
authors.lastname: Morsch
authors.affiliation: Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN, USA.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Machado
authors.affiliation: Department of Microbiology and Parasitology, UFSM, Santa Maria, RS, Brazil.
authors.forename: Matheus D
authors.initials: MD
authors.lastname: Baldissera
authors.affiliation: Department of Animal Science, Universidade do Estado de Santa Catarina (UDESC), Chapecó, SC, Brazil; Graduate Program in Animal Science, UDESC, Chapecó, SC, Brazil; Department of Biochemistry and Molecular Biology, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS, Brazil. Electronic address: dasilva.aleksandro@gmail.com.
authors.forename: Aleksandro S
authors.initials: AS
authors.lastname: Da Silva
mesh_terms: D000110:Acetylcholinesterase / Q000097:blood; D000818:Animals; D002091:Butyrylcholinesterase / Q000097:blood; D002800:Cholinesterase Inhibitors / Q000494:pharmacology; D002802:Cholinesterases / Q000097:blood*; D004492:Edetic Acid / Q000008:administration & dosage* / Q000494:pharmacology; D004789:Enzyme Activation; D005243:Feces / Q000469:parasitology; D006188:Haemonchiasis / Q000378:metabolism / Q000469:parasitology / Q000662:veterinary*; D006189:Haemonchus / Q000187:drug effects* / Q000502:physiology; D010058:Oviposition / Q000187:drug effects; D010270:Parasite Egg Count; D017382:Reactive Oxygen Species / Q000378:metabolism; D012756:Sheep; D012757:Sheep Diseases / Q000378:metabolism / Q000469:parasitology*; D013482:Superoxide Dismutase / Q000097:blood*
chemical_list: D002800:Cholinesterase Inhibitors; D017382:Reactive Oxygen Species; D004492:Edetic Acid; D013482:Superoxide Dismutase; D000110:Acetylcholinesterase; D002091:Butyrylcholinesterase; D002802:Cholinesterases
keywords: AChE; BChE; Copper edetate; Parasite; ROS