title: Scale-up production and characterization of anti-human cardiac troponin I monoclonal antibody in ascitic fluid of balb/c mice.
abstract: The Human Cardiac Troponin I (hcTnI) is a 210 amino acid protein, 23 kDa in molecular weight. This biomarker is commonly used to diagnose myocardial infarction, micro injury, and acute coronary syndrome (ACS) in patients referring to emergency departments. The American Heart Association (AHA) and European Society of Cardiology (ESC) proposed troponin I as the gold biomarker for early detection of heart attack, especially in myocardial infarction (MI). Therefore, developing monoclonal antibodies against this biomarker could help in for early detection of heart attack. Hybridoma technology is a well-known technique introduced to produce monoclonal antibodies in specialized cells. The aim of this study was to produce large scale of monoclonal antibody against human cardiac troponin I using Hybridoma technology in order to design a diagnostic kit. The monoclonal antibody was produced using conventional Hybridoma technology in ascitic fluid of mouse and characterized for its ability to detect Human Cardiac Troponin I in a rapid test system. The results indicate the successful detection of Troponin I using the obtained monoclonal antibody. According to the achieved results it seems that ascites production of monoclonal antibody is very versatile, inexpensive, and economically useful for monoclonal antibody production.
authors.affiliation: a Department of Clinical Biochemistry , Faculty of Medical Sciences, Tarbiat Modares University , Tehran , Iran.
authors.forename: Asiabanha Rezaee
authors.initials: AR
authors.lastname: Majid
authors.affiliation: a Department of Clinical Biochemistry , Faculty of Medical Sciences, Tarbiat Modares University , Tehran , Iran.
authors.forename: Rasaee Mohammad
authors.initials: RM
authors.lastname: Javad
authors.affiliation: b Department of Biochemistry, Faculty of Medical Sciences , Tehran University of Medical Sciences , Tehran , Iran.
authors.forename: Paknejad
authors.initials: P
authors.lastname: Malihe
authors.affiliation: c Department of Life Science Engineering, Faculty of New Sciences and Technologies , University of Tehran , Tehran , Iran.
authors.forename: Mohammadnejad
authors.initials: M
authors.lastname: Javad
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000276:immunology*; D001202:Ascitic Fluid / Q000276:immunology*; D006801:Humans; D051379:Mice; D008807:Mice, Inbred BALB C; D019210:Troponin I / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D019210:Troponin I
keywords: ascitic fluid; early detection; human cardiac troponin I; hybridoma technology