title: Switching cultivation for enhancing biomass and lipid production with extracellular polymeric substance as co-products in Heynigia riparia SX01.
abstract: Switching cultivation (mixotrophic-heterotrophic, 12h:12h) of Heynigia riparia SX01 was studied, the maximum biomass concentration of 3.55gL-1and lipid yield of 1.45gL-1were achieved after 8days cultivation. The extracellular polymeric substance (EPS) was developed as co-product. Addition of MgSO4could enhance the production of EPS. The highest amount of 0.60gL-1EPS was obtained with the addition of 2gL-1MgSO4, the self-flocculation efficiency was as high as 83% at this condition. The total lipid and lipid fractions did not show differences with extra MgSO4. Based on the above results, a new biodiesel production model was proposed: culturing Heynigia riparia SX01 with extra 2gL-1MgSO4by switching cultivation and using self flocculation to collect microalgae for biodiesel production, while EPS was collected as valuable co-products.
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Guodong
authors.initials: G
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Microbial Metabolism and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China; Biomass Energy Research Center, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: miaoxiaoling@sjtu.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Miao
mesh_terms: D056804:Biofuels / Q000382:microbiology; D018533:Biomass; D018929:Cell Culture Techniques / Q000379:methods*; D000460:Chlorophyta / Q000187:drug effects / Q000378:metabolism*; D005110:Extracellular Space / Q000737:chemistry*; D005227:Fatty Acids / Q000378:metabolism; D005947:Glucose / Q000494:pharmacology; D008055:Lipids / Q000096:biosynthesis*; D008278:Magnesium Sulfate / Q000494:pharmacology; D058086:Microalgae / Q000187:drug effects / Q000378:metabolism; D009005:Monosaccharides / Q000032:analysis; D011108:Polymers / Q000378:metabolism*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D056804:Biofuels; D005227:Fatty Acids; D008055:Lipids; D009005:Monosaccharides; D011108:Polymers; D008278:Magnesium Sulfate; D005947:Glucose
keywords: Biomass; Extracellular polymeric substance (EPSs); Heynigia riparia SX01; Lipid; Switching cultivation