title: Bacillussp. QSI-1 Modulate Quorum Sensing Signals ReduceAeromonas hydrophilaLevel and Alter Gut Microbial Community Structure in Fish.
abstract: Quorum sensing (QS) is a cell density dependent process that enables bacteria to communicate with each other based on the production, secretion and sensing of the auto-inducer molecules and then subsequently regulate virulence associated gene expression. Interrupting quorum sensing may represent a novel alternative approach to combat bacterial pathogen. Several bacteria can produce quorum quenching (QQ) enzymes. However, the role of QQ bacteria in shaping the microbiota and the level of N-acyl-homoserine lactones (AHLs, a prevalent type of QS molecules) producing bacteria remains largely unknown. The objective of this study was to examine the presence of AHLs in the fish intestine and investigate the modulation of gut microbiota and its effect onAeromonas hydrophilalevel by a QQ enzyme producing probioticBacillussp. QSI-1. AHLs were found in fish gut content and were confirmed inAeromonasspecies usingChromobacterium violaceumCV026 andAgrobacterium tumefaciensAT 136 (pZLR4) as reporter strains. We demonstrated that the composition of fish gut microbiota was affected by quenching bacteria QSI-1, and the percentage ofA. hydrophilawas decreased significantly. Taken together, these results provide valuable insights into QQ enzyme producing probiotics can modulate the microbiota structure and decrease the percentage of AHL-producing pathogenic bacteria in the gut. These data strongly suggest that QQ probiotics may serve as non-antibiotic feed additive in aquaculture to control bacterial diseases.
authors.affiliation: Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University Nanjing, China.
authors.forename: Shuxin
authors.initials: S
authors.lastname: Zhou
authors.affiliation: Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University Nanjing, China.
authors.forename: An
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University Nanjing, China.
authors.forename: Hongping
authors.initials: H
authors.lastname: Yin
authors.affiliation: Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University Nanjing, China.
authors.forename: Weihua
authors.initials: W
authors.lastname: Chu
mesh_terms: D054742:Acyl-Butyrolactones / Q000378:metabolism; D016980:Aeromonas hydrophila / Q000187:drug effects* / Q000235:genetics / Q000254:growth & development / Q000378:metabolism; D016960:Agrobacterium tumefaciens / Q000378:metabolism; D000818:Animals; D017756:Aquaculture / Q000379:methods; D001407:Bacillus / Q000201:enzymology / Q000254:growth & development / Q000378:metabolism*; D004269:DNA, Bacterial / Q000032:analysis; D004926:Escherichia coli / Q000187:drug effects / Q000235:genetics; D005260:Female; D005393:Fish Diseases / Q000178:diet therapy / Q000382:microbiology; D000069196:Gastrointestinal Microbiome / Q000502:physiology*; D006054:Goldfish / Q000382:microbiology*; D008049:Lipase / Q000378:metabolism; D008297:Male; D019936:Probiotics / Q000494:pharmacology; D053038:Quorum Sensing / Q000502:physiology*; D015398:Signal Transduction; D037521:Virulence Factors / Q000378:metabolism
chemical_list: D054742:Acyl-Butyrolactones; D004269:DNA, Bacterial; D037521:Virulence Factors; D008049:Lipase; C454481:lipase, Chromobacterium viscosum
keywords: A. hydrophila; N-acyl-homoserine lactones; gut microbiota; probiotics; quorum quenching