title: Measurement of amyloid formation by turbidity assay-seeing through the cloud.
abstract: Detection of amyloid growth is commonly carried out by measurement of solution turbidity, a low-cost assay procedure based on the intrinsic light scattering properties of the protein aggregate. Here, we review the biophysical chemistry associated with the turbidimetric assay methodology, exploring the reviewed literature using a series of pedagogical kinetic simulations. In turn, these simulations are used to interrogate the literature concerned with in vitro drug screening and the assessment of amyloid aggregation mechanisms.
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT, 2601, Australia.
authors.forename: Ran
authors.initials: R
authors.lastname: Zhao
authors.affiliation: Institute for Protein Research, Osaka University, 3-1- Yamada-oka, Suita, Osaka, 565-0871, Japan.
authors.forename: Masatomo
authors.initials: M
authors.lastname: So
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT, 2601, Australia.
authors.forename: Hendrik
authors.initials: H
authors.lastname: Maat
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT, 2601, Australia.
authors.forename: Nicholas J
authors.initials: NJ
authors.lastname: Ray
authors.affiliation: College of Bio-resource Sciences, Nihon University, Chiyoda-ku, Tokyo, 102-8275, Japan.
authors.forename: Fumio
authors.initials: F
authors.lastname: Arisaka
authors.affiliation: Institute for Protein Research, Osaka University, 3-1- Yamada-oka, Suita, Osaka, 565-0871, Japan.
authors.forename: Yuji
authors.initials: Y
authors.lastname: Goto
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT, 2601, Australia.
authors.forename: John A
authors.initials: JA
authors.lastname: Carver
authors.affiliation: Research School of Chemistry, Australian National University, Acton ACT, 2601, Australia. damien.hall@anu.edu.au.|Institute for Protein Research, Osaka University, 3-1- Yamada-oka, Suita, Osaka, 565-0871, Japan. damien.hall@anu.edu.au.
authors.forename: Damien
authors.initials: D
authors.lastname: Hall
keywords: Amyloid aggregation kinetics; Amyloid biophysics; Data reduction; Nonlinear signal response; Turbidimetric method