title: The acute effect of intravenous lipopolysaccharide injection on serum and intrafollicular HDL components and gene expression in granulosa cells of the bovine dominant follicle.
abstract: The aim of this study was to evaluate the effect of an acute systemic inflammatory response induced by lipopolysaccharide (LPS) in the serum and follicular fluid (FF) high-density lipoprotein (HDL) components, hormone concentrations and granulosa cell gene expression. For this purpose, twenty non-lactating Jersey dairy cows were submitted to a progesterone (P4) - estradiol (E2) based synchronization protocol. Cows received a single i.v. dose of LPS (2.5 μg/kg of body weight) or saline solution (CTL Group) 2 h after P4 insert removal. Blood, granulosa cells and FF samples were collected six hours after LPS injection. Five hours after LPS injection rectal temperature was increased in LPS (P < 0.0001, 40.4 ± 0.1 °C) compared to the CTL cows (38.8 ± 0.1 °C). Serum PON1 activity was reduced by LPS injection (130.2 ± 5.1 vs. 99.6 ± 3.3 U/mL; P < 0.001), as well as HDL-cholesterol concentrations (70.3 ± 5.3 vs. 50.1 ± 6.2 mg/dL; P < 0.05). The FF E2 and P4 concentrations were not different between groups (P > 0.05). The PON1 activity in the FF was also decreased by LPS injection (P = 0.01). In comparison to CTL group, cows injected with LPS had a ten fold reduction in STAR, TLR4 and TNF mRNA expression (P < 0.05). In conclusion, an intravenous LPS challenge in cows induced an acute systemic inflammatory response reducing HDL and its components in serum but not in the FF. Only PON1 activity serum reduction was reflected in the FF in the short term. Additionally, steroidogenic and inflammatory genes had reduced expression in the granulosa cells.
authors.affiliation: Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Felipe Terres
authors.initials: FT
authors.lastname: de Campos
authors.affiliation: Department of Veterinary Clinics, Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Joao Aveiro Alvarado
authors.initials: JAA
authors.lastname: Rincon
authors.affiliation: Corporación Colombiana de Investigación Agropecuaria (CORPOICA), Bogota, Colombia.
authors.forename: Diego Andres Velasco
authors.initials: DAV
authors.lastname: Acosta
authors.affiliation: Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Pedro Augusto Silva
authors.initials: PAS
authors.lastname: Silveira
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Clima Temperado, Pelotas, RS, Brazil.
authors.forename: Jorgea
authors.initials: J
authors.lastname: Pradieé
authors.affiliation: Department of Veterinary Clinics, Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Marcio Nunes
authors.initials: MN
authors.lastname: Corrêa
authors.affiliation: Department of Pathology, Veterinary College, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Bernardo Garziera
authors.initials: BG
authors.lastname: Gasperin
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Porto Velho, RO, Brazil.
authors.forename: Luiz Francisco Machado
authors.initials: LFM
authors.lastname: Pfeifer
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil.
authors.forename: Carlos Castilho
authors.initials: CC
authors.lastname: Barros
authors.affiliation: Empresa Brasileira de Pesquisa Agropecuária (Embrapa) Clima Temperado, Pelotas, RS, Brazil.
authors.forename: Ligia Margareth Cantareli
authors.initials: LMC
authors.lastname: Pegoraro
authors.affiliation: Department of Nutrition, College of Nutrition, Universidade Federal de Pelotas, Pelotas, RS, Brazil. Electronic address: augusto.schneider@ufpel.edu.br.
authors.forename: Augusto
authors.initials: A
authors.lastname: Schneider
mesh_terms: D061605:Administration, Intravenous; D000818:Animals; D043303:Aryldialkylphosphatase / Q000235:genetics / Q000378:metabolism; D002417:Cattle; D004973:Estrus Synchronization; D005260:Female; D015571:Follicular Fluid / Q000378:metabolism; D005786:Gene Expression Regulation / Q000187:drug effects*; D006107:Granulosa Cells / Q000187:drug effects / Q000378:metabolism; D008070:Lipopolysaccharides / Q000008:administration & dosage / Q000494:pharmacology*; D008075:Lipoproteins, HDL / Q000378:metabolism*; D006080:Ovarian Follicle / Q000187:drug effects / Q000378:metabolism*
chemical_list: D008070:Lipopolysaccharides; D008075:Lipoproteins, HDL; D043303:Aryldialkylphosphatase
keywords: Cholesterol; Inflammation; PON1; Steroidogenesis