title: SETD4 Regulates Cell Quiescence and Catalyzes the Trimethylation of H4K20 during Diapause Formation in Artemia.
abstract: As a prominent characteristic of cell life, the regulation of cell quiescence is important for proper development, regeneration, and stress resistance and may play a role in certain degenerative diseases. However, the mechanism underlying quiescence remains largely unknown. Encysted embryos ofArtemiaare useful for studying the regulation of this state because they remain quiescent for prolonged periods during diapause, a state of obligate dormancy. In the present study, SET domain-containing protein 4, a histone lysine methyltransferase fromArtemia, was identified, characterized, and named Ar-SETD4. We found that Ar-SETD4 was expressed abundantly inArtemiadiapause embryos, in which cells were in a quiescent state. Meanwhile, trimethylated histone H4K20 (H4K20me3) was enriched in diapause embryos. The knockdown of Ar-SETD4 reduced the level of H4K20me3 significantly and prevented the formation of diapause embryos in which neither the cell cycle nor embryogenesis ceased. The catalytic activity of Ar-SETD4 on H4K20me3 was confirmed by anin vitrohistone methyltransferase (HMT) assay and overexpression in cell lines. This study provides insights into the function of SETD4 and the mechanism of cell quiescence regulation.
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Dai
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Ye
authors.affiliation: School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China.
authors.forename: Hua-Wei
authors.initials: HW
authors.lastname: Li
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Dian-Fu
authors.initials: DF
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Hong-Liang
authors.initials: HL
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Sheng-Nan
authors.initials: SN
authors.lastname: Jia
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lin
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Jin-Shu
authors.initials: JS
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China.|Department of Biological Chemistry, The University of Tokyo, Tokyo, Japan.
authors.forename: Hiromichi
authors.initials: H
authors.lastname: Nagasawa
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, Zhejiang, China w_jyang@zju.edu.cn.
authors.forename: Wei-Jun
authors.initials: WJ
authors.lastname: Yang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001156:Artemia / Q000166:cytology / Q000196:embryology* / Q000378:metabolism*; D001483:Base Sequence; D055162:Biocatalysis; D002453:Cell Cycle; D002455:Cell Division; D045744:Cell Line, Tumor; D064666:Diapause, Insect; D004625:Embryo, Nonmammalian / Q000166:cytology / Q000378:metabolism; D055785:Gene Knockdown Techniques; D006657:Histones / Q000378:metabolism*; D008239:Lysine / Q000378:metabolism*; D008745:Methylation; D014157:Transcription Factors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D006657:Histones; D014157:Transcription Factors; D008239:Lysine
keywords: Artemia; H4K20me3; SETD4; cell quiescence; diapause