title: Bithalamic gliomas may be molecularly distinct from their unilateral high-grade counterparts.
abstract: Bithalamic gliomas are rare cancers diagnosed based on poorly defined radiologic criteria. Infiltrative astrocytomas account for most cases. While some previous studies reported dismal outcomes for patients with bithalamic gliomas irrespective of therapy and histologic grade, others described better prognoses even without anticancer therapy. Little is known about their molecular characteristics. We reviewed clinical, radiologic, and histologic features of patients with bithalamic gliomas treated at our institution over 15 years. Targeted sequencing of mutational hotspots in H3F3A, HIST1H3B, IDH1/2, and BRAF, and genome-wide analysis of DNA methylation and copy number abnormalities was performed in available tumors. Eleven patients with bithalamic gliomas were identified. Their median age at diagnosis was 4.8 years (range: 1-15.7). Additional involvement of the brainstem, basal ganglia, and cerebral lobes occurred in 11, 9, and 3 cases, respectively. All patients presented with hydrocephalus. Two-thirds of the patients had a histologic diagnosis of anaplastic astrocytoma. Despite aggressive therapy, our youngest patient, the only one diagnosed before 1 year of age, is the sole long-term survivor. DNA methylation could be performed in seven tumors, all of which clustered with the RTK I 'PDGFRA' subgroup by unsupervised hierarchical analysis of methylation array against a previously published cohort of 59 pediatric high-grade gliomas. Sequencing of hotspots mutations could be done in 10 tumors, none of which harbored H3F3A p.K27 and/or the respective DNA methylation signature, and any other hotspot mutations. Amplification of MDM4 (n = 2), PDGFRA (n = 2), and ID2 combined with MYCN (n = 1) were observed in 7 tumors available for analysis. In comparison with the previously published experience with unilateral high-grade thalamic astrocytomas where H3F3A p.K27 was present in two-thirds of cases, the absence of this molecular subgroup in bithalamic gliomas was striking. This finding suggests that unilateral and bithalamic high-grade gliomas may represent two distinct molecular entities.
authors.affiliation: Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN.|Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN.
authors.forename: Alberto
authors.initials: A
authors.lastname: Broniscer
authors.affiliation: Department of Diagnostic Imaging, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Scott N
authors.initials: SN
authors.lastname: Hwang
authors.affiliation: Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Omar
authors.initials: O
authors.lastname: Chamdine
authors.affiliation: Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Tong
authors.initials: T
authors.lastname: Lin
authors.affiliation: Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Stanley
authors.initials: S
authors.lastname: Pounds
authors.affiliation: Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Arzu
authors.initials: A
authors.lastname: Onar-Thomas
authors.affiliation: Department of Biostatistics, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Lei
authors.initials: L
authors.lastname: Chi
authors.affiliation: Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Sheila
authors.initials: S
authors.lastname: Shurtleff
authors.affiliation: Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Sariah
authors.initials: S
authors.lastname: Allen
authors.affiliation: Department of Oncology, St. Jude Children's Research Hospital, Memphis, TN.|Department of Pediatrics, University of Tennessee Health Science Center, Memphis, TN.
authors.forename: Amar
authors.initials: A
authors.lastname: Gajjar
authors.affiliation: Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Paul
authors.initials: P
authors.lastname: Northcott
authors.affiliation: Department of Pathology, St. Jude Children's Research Hospital, Memphis, TN.
authors.forename: Brent A
authors.initials: BA
authors.lastname: Orr
mesh_terms: D000293:Adolescent; D001921:Brain / Q000000981:diagnostic imaging / Q000378:metabolism / Q000473:pathology; D001932:Brain Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology* / Q000628:therapy; D002648:Child; D002675:Child, Preschool; D019175:DNA Methylation; D005260:Female; D005910:Glioma / Q000235:genetics / Q000378:metabolism* / Q000473:pathology* / Q000628:therapy; D006801:Humans; D017404:In Situ Hybridization, Fluorescence; D007223:Infant; D008297:Male; D060787:Neoplasm Grading; D012189:Retrospective Studies; D016896:Treatment Outcome
keywords: bithalamic; children; high-grade glioma; infiltrative; molecular characteristics