title: miR-382 inhibits tumor progression by targeting SETD8 in non-small cell lung cancer.
abstract: Previous studies showed that miR-382 plays important roles in several types of cancers. Nevertheless, its expression and function in non-small cell lung cancer (NSCLC) remains largely unknown. In this study, we found that miR-382 expression was evidently downregulated in NSCLC tissue and cell lines in comparison with the adjacent normal tissues and human bronchial epithelial cell line (16HBE). Moreover, the expression levels of miR-382 were significantly associated with last-stage and tumor metastasis in NSCLC patients. In addition, exogenous miR-382 evidently inhibited NSCLC cell proliferation, migration and invasion in vitro. We also revealed SETD8 as a direct target of miR-382 in NSCLC, and restored SETD8 partially reversed the negative effects miR-382 on NSCLC cells. In total, our study demonstrated that miR-382 dysregulated in NSCLC and involved in NSCLC tumorigenesis and metastasis by suppressing SETD8 expression, which may help to identify effective therapies for NSCLC treatment.
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: tjchen223@163.com.
authors.forename: Tianjun
authors.initials: T
authors.lastname: Chen
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: hren223@163.com.
authors.forename: Hui
authors.initials: H
authors.lastname: Ren
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: thakur223@163.com.
authors.forename: Asmitanand
authors.initials: A
authors.lastname: Thakur
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: tyang223@163.com.
authors.forename: Tian
authors.initials: T
authors.lastname: Yang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: yangli223a@163.com.
authors.forename: Yang
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: sqzhang585@163.com.
authors.forename: Shuo
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: yczhang546@163.com.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Respiratory, The First Affiliated Hospital, Xi'an Jiaotong University, Xi'an, Shaanxi 710061, PR China. Electronic address: mwchen223@163.com.
authors.forename: Mingwei
authors.initials: M
authors.lastname: Chen
mesh_terms: D000368:Aged; D002289:Carcinoma, Non-Small-Cell Lung / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects / Q000502:physiology; D005260:Female; D018390:Gene Targeting / Q000379:methods*; D011495:Histone-Lysine N-Methyltransferase / Q000096:biosynthesis / Q000235:genetics*; D006801:Humans; D008175:Lung Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000473:pathology / Q000517:prevention & control
chemical_list: C555413:MIRN382 microRNA, human; D035683:MicroRNAs; D011495:Histone-Lysine N-Methyltransferase; C470730:KMT5A protein, human
keywords: Invasion; Migration; Non-small cell lung cancer; Proliferation; SETD8; miR-382