title: Role of neuropeptide F in regulating filter feeding of Manila clam, Ruditapes philippinarum.
abstract: Endogenous signals which may be involved in the regulation of filter feeding in bivalves have never been examined. NPY/NPF homologue has been proved to play an important role in the regulation of food intake in vertebrate and several invertebrates. In this study, a NPF homologue was cloned from visceral ganglia of clam Ruditapes philippinarum. The full-length cDNA sequence was 892bp in length and encoded a precursor of 82 amino acid residues. We then examined the effects of fasting and refeeding on the filtration rates (FR), plasma glucose concentration (PGC), 5-HT, DA and the expression level of the rp-NPF and insulin transcript. The mRNA expression level of rp-NPF in visceral ganglion was increased during fasting, and rose to highest level on 72h after starvation and declined immediately after food had been supplied. Hemocoel injection of rp-NPF(5μg/g)significantly increased FR of clams within 2h. Compared to the controls, a significant increase in insulin mRNA levels was observed at 8h after injection. Contents of 5-HT and DA also increased in the 5μg/grp-NPF administrated clams at 8 and 24h after injection. These results suggest that, similar to vertebrates, NPF, insulin, 5-HT and DA may play a role in the regulation of feeding in R. philippinarum.
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China. Electronic address: jmiao@ouc.edu.cn.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Miao
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Liu
authors.affiliation: The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, PR China.
authors.forename: Luqing
authors.initials: L
authors.lastname: Pan
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D049872:Bivalvia / Q000235:genetics* / Q000378:metabolism / Q000502:physiology*; D003001:Cloning, Molecular; D004435:Eating; D005215:Fasting; D005786:Gene Expression Regulation; D006801:Humans; D009479:Neuropeptides / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism*; D012689:Sequence Homology, Nucleic Acid
chemical_list: D009479:Neuropeptides; C076101:neuropeptide F
keywords: Biogenic amines; Filter feeding; Insulin; Neuropeptide F; Ruditapes philippinarum