title: Comparative Analysis of Satellite DNA in theDrosophila melanogasterSpecies Complex.
abstract: Satellite DNAs are highly repetitive sequences that account for the majority of constitutive heterochromatin in many eukaryotic genomes. It is widely recognized that sequences and locations of satellite DNAs are highly divergent even in closely related species, contributing to the hypothesis that satellite DNA differences may underlie speciation. However, due to its repetitive nature, the mapping of satellite DNAs has been mostly left out of recent genomics analyses, hampering the use of molecular genetics techniques to better understand their role in speciation and evolution. Satellite DNAs are most extensively and comprehensively mapped inDrosophila melanogaster, a species that is also an excellent model system with which to study speciation. Yet the lack of comprehensive knowledge regarding satellite DNA identity and location in its sibling species (D. simulans,D. mauritiana, andD. sechellia) has prevented the full utilization ofD. melanogasterin studying speciation. To overcome this problem, we initiated the mapping of satellite DNAs on the genomes of theD. melanogasterspecies complex (D. melanogaster,D. simulans,D. mauritiana, andD. sechellia) using multi-color fluorescentin situhybridization (FISH) probes. Our study confirms a striking divergence of satellite DNAs in theD. melanogasterspecies complex, even among the closely related species of theD. simulansclade (D. simulans,D. mauritiana, andD. sechellia), and suggests the presence of unidentified satellite sequences in these species.
authors.affiliation: Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan 48109.|Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109.
authors.forename: Madhav
authors.identifier: 0000-0003-3428-6812
authors.initials: M
authors.lastname: Jagannathan
authors.affiliation: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109.|Department of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, Michigan 48109.
authors.forename: Natalie
authors.identifier: 0000-0002-9375-8990
authors.initials: N
authors.lastname: Warsinger-Pepe
authors.affiliation: Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan 48109.|Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109.
authors.forename: George J
authors.identifier: 0000-0001-8250-9027
authors.initials: GJ
authors.lastname: Watase
authors.affiliation: Howard Hughes Medical Institute, University of Michigan, Ann Arbor, Michigan 48109 yukikomy@umich.edu.|Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109.|Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan 48109.
authors.forename: Yukiko M
authors.identifier: 0000-0001-5541-0216
authors.initials: YM
authors.lastname: Yamashita
mesh_terms: D000818:Animals; D002874:Chromosome Mapping; D004276:DNA, Satellite / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics*; D019143:Evolution, Molecular; D049810:Genetic Speciation; D006570:Heterochromatin / Q000235:genetics; D017404:In Situ Hybridization, Fluorescence; D012091:Repetitive Sequences, Nucleic Acid / Q000235:genetics*; D013045:Species Specificity
chemical_list: D004276:DNA, Satellite; D006570:Heterochromatin
keywords: Drosophila; evolution; fluorescent in situ hybridization; heterochromatin; satellite DNA