title: Selected fatty acids as biomarkers of exposure to microdoses of molluscicides in snails Helix pomatia (Gastropoda Pulmonata).
abstract: Two stages of selection from a pool of 56 fatty acids analyzed in Helix pomatia yielded a set of 12 biomarker acids undergoing significant changes in contact with three microdoses of toxic substances, i.e. three molluscicides containing metaldehyde, methiocarb, and potassium chloride (PC). The proposed palette of acids, including saturated fatty acids (SFA), monounsaturated fatty acids (MUFA), and polyunsaturated fatty acids (PUFA), determined separately in the foot tissues and hepatopancreas of Gastropoda, can be used in ecotoxicological research as a reliable test of the effect of trace doses of stressors. The final set of the biomarker FA comprised C16:0; C18:0; C23:0; C18:1 n-9; C20:1 n-9; C18:2 n-6; C18:3 n-3; C20:2; C20:4 n-6; C20:5 n-3; C22:4 n-6; and C22:5 n-3. A clear physiological response manifested as changes in the content of fatty acids (FA) was observed in the snails even in the case of the lowest doses of the pollutants. All experimental factors analyzed, i.e., the dose (5, 10, or 15 μl 0.01% w/v concentration) and the type of preparation (metaldehyde, methiocarb or PC), had a significant (p ≤ 0.01) impact on the FA composition of the foot and hepatopancreas. Limitation of the analysis to a narrow pool of reactive FA meets the requirements of parameters of biomarkers of exposure and facilitates and accelerates visualization of the bioindicator organism's response to the presence of the stressor in the environment.
authors.affiliation: Department of Zoology, Animal Ecology and Wildlife Management, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland. Electronic address: danakp@wp.pl.
authors.forename: Danuta
authors.initials: D
authors.lastname: Kowalczyk-Pecka
authors.affiliation: Institute of Animal Nutrition and Bromatology, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
authors.forename: Stanisław
authors.initials: S
authors.lastname: Pecka
authors.affiliation: Institute of Animal Nutrition and Bromatology, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Kowalczuk-Vasilev
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D004781:Environmental Exposure / Q000032:analysis*; D005227:Fatty Acids / Q000032:analysis / Q000378:metabolism*; D050356:Lipid Metabolism / Q000187:drug effects; D008975:Molluscacides / Q000494:pharmacology*; D011044:Poland; D012908:Snails / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D005227:Fatty Acids; D008975:Molluscacides
keywords: Biomarker; Fatty acid; Molluscicide; Snail; Xenobiotic