title: Wide-field spontaneous Raman spectroscopy imaging system for biological tissue interrogation.
abstract: Raman spectroscopy has shown great promise as a method to discriminate between cancerous and normal tissue/cells for a range of oncology applications using microscopy and tissue interrogation instruments such as handheld probes and needles. Here we are presenting preliminary steps toward the development of a practical handheld macroscopic Raman spectroscopy instrument, demonstrating its capabilities to discriminate between different biological tissue types during ex vivo porcine experiments. The novel probe design can image a field of view of 25  mm2with a spatial resolution <100  μm and an average spectral resolution of 95  cm-1, covering the fingerprint region between 450 to 1750  cm-1. The ability of the system to produce tissue maps based on molecular characteristics is demonstrated using a neural network machine learning technique.
authors.forename: K
authors.initials: K
authors.lastname: St-Arnaud
authors.forename: K
authors.initials: K
authors.lastname: Aubertin
authors.forename: M
authors.initials: M
authors.lastname: Strupler
authors.forename: M
authors.initials: M
authors.lastname: Jermyn
authors.forename: K
authors.initials: K
authors.lastname: Petrecca
authors.forename: D
authors.initials: D
authors.lastname: Trudel
authors.forename: F
authors.initials: F
authors.lastname: Leblond
mesh_terms: D000818:Animals; D003952:Diagnostic Imaging; D004867:Equipment Design; D016571:Neural Networks, Computer; D013059:Spectrum Analysis, Raman / Q000295:instrumentation*; D013552:Swine