title: Fine Mapping ofUr-3, a Historically Important Rust Resistance Locus in Common Bean.
abstract: Bean rust, caused byUromyces appendiculatus, is a devastating disease of common bean (Phaseolus vulgaris) in the Americas and Africa. The historically importantUr-3gene confers resistance to many races of the highly variable bean rust pathogen that overcome other rust resistance genes. Existing molecular markers taggingUr-3for use in marker-assisted selection produce false results. Here, we describe the fine mapping of theUr-3locus for the development of highly accurate markers linked toUr-3An F2population from the cross Pinto 114 (susceptible) × Aurora (resistant withUr-3) was evaluated for its reaction to four different races ofU. appendiculatusA bulked segregant analysis using the SNP chip BARCBEAN6K_3 placed the approximate location ofUr-3in the lower arm of chromosome Pv11. Specific SSR and SNP markers and haplotype analysis of 18 sequenced bean varieties positionedUr-3in a 46.5 kb genomic region from 46.96 to 47.01 Mb on Pv11. We discovered in this region the SS68 KASP marker that was tightly linked toUr-3Validation of SS68 on a panel of 130 diverse common bean cultivars containing all known rust resistance genes revealed that SS68 was highly accurate and produced no false results. The SS68 marker will be of great value in pyramidingUr-3with other rust resistance genes. It will also significantly reduce time and labor associated with the current phenotypic detection ofUr-3This is the first utilization of fine mapping to discover markers linked to rust resistance in common bean.
authors.affiliation: Soybean Genomics and Improvement Laboratory, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center-West, Maryland 20705.
authors.forename: Oscar P
authors.initials: OP
authors.lastname: Hurtado-Gonzales
authors.affiliation: Departamento de Agronomia, Universidade Estadual de Maringá, PR 87020900, Brazil.
authors.forename: Giseli
authors.initials: G
authors.lastname: Valentini
authors.affiliation: Departamento de Agronomia, Universidade Estadual de Maringá, PR 87020900, Brazil.
authors.forename: Thiago A S
authors.initials: TA
authors.lastname: Gilio
authors.affiliation: Coordenação de Tecnologia em Educacao a Distancia, Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior, Quadra St. Bancário Norte, Brasília, DF 70040020, Brazil.
authors.forename: Alexandre M
authors.initials: AM
authors.lastname: Martins
authors.affiliation: Soybean Genomics and Improvement Laboratory, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center-West, Maryland 20705.
authors.forename: Qijian
authors.initials: Q
authors.lastname: Song
authors.affiliation: Soybean Genomics and Improvement Laboratory, United States Department of Agriculture-Agricultural Research Service, Beltsville Agricultural Research Center-West, Maryland 20705 talo.pastor-corrales@ars.usda.gov.
authors.forename: Marcial A
authors.initials: MA
authors.lastname: Pastor-Corrales
mesh_terms: D000349:Africa; D000569:Americas; D001487:Basidiomycota / Q000472:pathogenicity; D002874:Chromosome Mapping; D060467:Disease Resistance / Q000235:genetics*; D008040:Genetic Linkage; D006239:Haplotypes; D018895:Microsatellite Repeats / Q000235:genetics; D020411:Oligonucleotide Array Sequence Analysis; D027805:Phaseolus / Q000235:genetics* / Q000382:microbiology; D010935:Plant Diseases / Q000235:genetics* / Q000382:microbiology; D010940:Plant Proteins / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics
chemical_list: D010940:Plant Proteins
keywords: KASP marker; Phaseolus vulgaris; Uromyces appendiculatus; fine mapping; rust resistance gene