title: Determination of Structure and Micellar Interactions of Small Antimicrobial Peptides by Solution-State NMR.
abstract: NMR spectroscopy is a well-established technique to determine the structure of peptides and small proteins in solution, also when bound to detergent micelles or phospholipid bicelles. The structure of the peptide alone is, however, not conveying the full picture, if the peptide is bound to a micelle, since it does not tell anything about the orientation of the peptide in the micelle. This article describes how to obtain that information together with information on peptide structure.
authors.affiliation: Department of Chemistry and Bioscience, University of Aalborg, Frederik Bajers vej 7H, 9220, Aalborg, Denmark. rw@bio.aau.dk.
authors.forename: Reinhard
authors.initials: R
authors.lastname: Wimmer
authors.affiliation: Department of Chemistry and Bioscience, University of Aalborg, Frederik Bajers vej 7H, 9220, Aalborg, Denmark.
authors.forename: Lars Erik
authors.initials: LE
authors.lastname: Uggerhøj
mesh_terms: D000595:Amino Acid Sequence; D000596:Amino Acids / Q000737:chemistry; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry* / Q000378:metabolism; D057846:Cell-Penetrating Peptides / Q000737:chemistry / Q000378:metabolism; D008055:Lipids / Q000737:chemistry; D008823:Micelles; D008958:Models, Molecular; D008968:Molecular Conformation; D015394:Molecular Structure; D019906:Nuclear Magnetic Resonance, Biomolecular; D011485:Protein Binding; D012996:Solutions
chemical_list: D000596:Amino Acids; D023181:Antimicrobial Cationic Peptides; D057846:Cell-Penetrating Peptides; D008055:Lipids; D008823:Micelles; D012996:Solutions
keywords: Antimicrobial peptides; Cell-penetrating peptides; NMR; Paramagnetic relaxation enhancement; Peptide-lipid interactions