title: Molecular mechanisms underlying active desalination and low water permeability in the esophagus of eels acclimated to seawater.
abstract: Marine teleosts can absorb imbibed seawater (SW) to maintain water balance, with esophageal desalination playing an essential role. NaCl absorption from luminal SW was enhanced 10-fold in the esophagus of SW-acclimated eels, and removal of Na+or Cl-from luminal SW abolished the facilitated absorption, indicating coupled transport. Mucosal/serosal application of various blockers for Na+/Cl-transporters profoundly decreased the absorption. Among the transporter genes expressed in eel esophagus detected by RNA-seq, dimethyl amiloride-sensitive Na+/H+exchanger (NHE3) and 4,4'-diisothiocyano-2,2'-disulfonic acid-sensitive Cl-/[Formula: see text] exchanger (AE) coupled by the scaffolding protein on the apical membrane of epithelial cells, and ouabain-sensitive Na+-K+-ATPases (NKA1α1c and NKA3α) and diphenylamine-2-carboxylic acid-sensitive Cl-channel (CLCN2) on the basolateral membrane, may be responsible for enhanced transcellular NaCl transport because of their profound upregulation after SW acclimation. Upregulated carbonic anhydrase 2a (CA2a) supplies H+and [Formula: see text] for activation of the coupled NHE and AE. Apical hydrochlorothiazide-sensitive Na+-Cl-cotransporters and basolateral Na+-[Formula: see text] cotransporter (NBCe1) and AE1 are other possible candidates. Concerning the low water permeability that is typically seen in marine teleost esophagus, downregulated aquaporin genes (aqp1aandaqp3) and upregulated claudin gene (cldn15a) are candidates for transcellular/paracellular route. In situ hybridization showed that these upregulated transporters and tight-junction protein genes were expressed in the absorptive columnar epithelial cells of eel esophagus. These results allow us to provide a full picture of the molecular mechanism of active desalination and low water permeability that are characteristic to marine teleost esophagus and gain deeper insights into the role of gastrointestinal tracts in SW acclimation.
authors.affiliation: Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan; takei@aori.u-tokyo.ac.jp.
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Takei
authors.affiliation: Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Marty K-S
authors.initials: MK
authors.lastname: Wong
authors.affiliation: Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Supriya
authors.initials: S
authors.lastname: Pipil
authors.affiliation: Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan.|Bioinformatics Research Unit, Advanced Center for Computing and Communication, RIKEN, Wako, Saitama, Japan; and.
authors.forename: Haruka
authors.initials: H
authors.lastname: Ozaki
authors.affiliation: Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Suzuki
authors.affiliation: Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwa, Chiba, Japan.|Department of Biological Sciences, Graduate School of Science, University of Tokyo, Bunkyo-ku, Tokyo, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Iwasaki
authors.affiliation: Laboratory of Physiology, Atmosphere and Ocean Research Institute, University of Tokyo, Kashiwa, Chiba, Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Kusakabe
mesh_terms: D000818:Animals; D002463:Cell Membrane Permeability / Q000502:physiology; D004524:Eels / Q000502:physiology*; D004947:Esophagus / Q000502:physiology*; D065569:Gastrointestinal Absorption / Q000502:physiology*; D015640:Ion Channel Gating / Q000502:physiology; D000068357:Saline Waters / Q000493:pharmacokinetics*; D055049:Salt Tolerance / Q000502:physiology*; D012623:Seawater; D012965:Sodium Chloride / Q000493:pharmacokinetics; D028021:Sodium-Potassium-Chloride Symporters / Q000502:physiology*
chemical_list: D028021:Sodium-Potassium-Chloride Symporters; D012965:Sodium Chloride
keywords: digestive tracts; epithelial transport; seawater acclimation; teleost fish