title: β-Ag2MoO4microcrystals: Characterization, antibacterial properties and modulation analysis of antibiotic activity.
abstract: This study reports the antibacterial properties and modulation analysis of antibiotic activity by β-Ag2MoO4microcrystals as well as their structural and vibrational characterization. The silver molybdate was obtained by the conventional hydrothermal method, and the structural, vibrational and morphological properties of the sample were determined using X-ray diffraction, Raman spectroscopy and scanning electron microscopy images. β-Ag2MoO4microcrystals obtained show spinel-type cubic structure (Fd-3m) with irregular shapes. The evaluation of antibacterial and modulatory-antibiotic activity was performed using the microdilution method to determine the Minimum Inhibitory Concentration (MIC) of the β-Ag2MoO4and antibiotics alone and associated with the silver molybdate. The β-Ag2MoO4modulates the antibiotic activity against all bacteria assayed in a synergistic (as the norfloxacin and gentamicin against S. aureus and gentamicin against E. coli) or an antagonistic form (as the norfloxacin against E.coli and P. aeruginosa). The reversion of antibiotic resistance by combinations with Ag2MoO4could be a novel strategy to combat infections caused by multiple drug resistance (MDR) pathogens. Our results indicate that these silver molybdates present a clinically relevant antibacterial activity and enhanced the antibiotic activity of some antibiotics against MDR strain of S. aureus and E. coli, being an interesting alternative to combat antibiotic-resistant bacterial infectious agents.
authors.affiliation: Departamento de Física, Universidade Federal do Ceará, P. O. Box 6030, CEP 60455-970 Fortaleza, CE, Brazil; Universidade Federal do Cariri, CEP 63000-000 Juazeiro do Norte, CE, Brazil. Electronic address: victor.moura@fisica.ufc.br.
authors.forename: J V B
authors.initials: JV
authors.lastname: Moura
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, CEP 63105-000 Crato, CE, Brazil.
authors.forename: T S
authors.initials: TS
authors.lastname: Freitas
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, CEP 63105-000 Crato, CE, Brazil.
authors.forename: R P
authors.initials: RP
authors.lastname: Cruz
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, CEP 63105-000 Crato, CE, Brazil.
authors.forename: R L S
authors.initials: RL
authors.lastname: Pereira
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, CEP 63105-000 Crato, CE, Brazil.
authors.forename: A R P
authors.initials: AR
authors.lastname: Silva
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, CEP 63105-000 Crato, CE, Brazil.
authors.forename: A T L
authors.initials: AT
authors.lastname: Santos
authors.affiliation: Universidade Federal do Cariri, CEP 63000-000 Juazeiro do Norte, CE, Brazil.
authors.forename: J H
authors.initials: JH
authors.lastname: da Silva
authors.affiliation: Departamento de Física, Campus Ministro Petrônio Portella, Universidade Federal do Piauí, CEP 64049-550 Teresina, PI, Brazil.
authors.forename: C
authors.initials: C
authors.lastname: Luz-Lima
authors.affiliation: Departamento de Física, Universidade Federal do Ceará, P. O. Box 6030, CEP 60455-970 Fortaleza, CE, Brazil.
authors.forename: P T C
authors.initials: PT
authors.lastname: Freire
authors.affiliation: Laboratório de Microbiologia e Biologia Molecular, Universidade Regional do Cariri, CEP 63105-000 Crato, CE, Brazil.
authors.forename: H D M
authors.initials: HD
authors.lastname: Coutinho
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry* / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D006801:Humans; D007454:Iodides / Q000737:chemistry* / Q000494:pharmacology*; D008826:Microbial Sensitivity Tests / Q000379:methods; D008863:Microspheres; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D018030:Silver Compounds / Q000737:chemistry* / Q000494:pharmacology*; D013211:Staphylococcus aureus / Q000187:drug effects; D014961:X-Ray Diffraction / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D007454:Iodides; D018030:Silver Compounds; C030584:silver iodide
keywords: Antibacterial properties; Raman spectroscopy; Silver molybdate; X-ray diffraction