title: Bacillus subtilis iolU encodes an additional NADP+-dependent scyllo-inositol dehydrogenase.
abstract: Bacillus subtilis genes iolG, iolW, iolX, ntdC, yfiI, yrbE, yteT, and yulF belong to the Gfo/Idh/MocA family. The functions of iolG, iolW, iolX, and ntdC are known; however, the functions of the others are unknown. We previously reported the B. subtilis cell factory simultaneously overexpressing iolG and iolW to achieve bioconversion of myo-inositol (MI) into scyllo-inositol (SI). YulF shares a significant similarity with IolW, the NADP+-dependent SI dehydrogenase. Transcriptional abundance of yulF did not correlate to that of iol genes involved in inositol metabolism. However, when yulF was overexpressed instead of iolW in the B. subtilis cell factory, SI was produced from MI, suggesting a similar function to iolW. In addition, we demonstrated that recombinant His6-tagged YulF converted scyllo-inosose into SI in an NADPH-dependent manner. We have thus identified yulF encoding an additional NADP+-dependent SI dehydrogenase, which we propose to rename iolU.
authors.affiliation: a Department of Agrobioscience , Graduate School of Agricultural Science, Kobe University , Kobe , Japan.
authors.forename: Dong-Min
authors.initials: DM
authors.lastname: Kang
authors.affiliation: b Organization of Advanced Science and Technology , Kobe University , Kobe , Japan.
authors.forename: Kosei
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: a Department of Agrobioscience , Graduate School of Agricultural Science, Kobe University , Kobe , Japan.|b Organization of Advanced Science and Technology , Kobe University , Kobe , Japan.
authors.forename: Shinji
authors.initials: S
authors.lastname: Takenaka
authors.affiliation: c Department of Science, Technology and Innovation, Graduate School of Science, Technology and Innovation , Kobe University , Kobe , Japan.
authors.forename: Shu
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: b Organization of Advanced Science and Technology , Kobe University , Kobe , Japan.|c Department of Science, Technology and Innovation, Graduate School of Science, Technology and Innovation , Kobe University , Kobe , Japan.
authors.forename: Ken-Ichi
authors.initials: KI
authors.lastname: Yoshida
mesh_terms: D001412:Bacillus subtilis / Q000201:enzymology* / Q000235:genetics*; D004789:Enzyme Activation; D007294:Inositol / Q000378:metabolism*; D009249:NADP / Q000378:metabolism*; D013401:Sugar Alcohol Dehydrogenases / Q000235:genetics* / Q000378:metabolism*
chemical_list: C009217:scyllitol; D007294:Inositol; D009249:NADP; D013401:Sugar Alcohol Dehydrogenases
keywords: Bacillus subtilis; NADP+/NADPH; inositol dehydrogenase; myo-inositol; scyllo-inositol