title: Chondrogenic differentiation of ATDC5-cells under the influence of Mg and Mg alloy degradation.
abstract: Biodegradable magnesium (Mg)-based materials are a potential alternative to permanent implants for application in children. Nevertheless effects of those materials on growth plate cartilage and chondrogenesis have not been previously evaluated. In vitro differentiation of ATDC5 cells was evaluated under the influence of pure Mg (PMg), Mg with 10wt% of gadolinium (Mg-10Gd) and Mg with 2wt% of silver (Mg-2Ag) degradation products (extracts) and direct cell culture on the materials. Gene expression showed an inhibitory effect on ATDC5 mineralization with the three extracts and a chondrogenic potential of Mg-10Gd. Cells cultured in Mg-10Gd and Mg-2Ag extracts showed the same proliferation and morphology than cells cultured in growth conditions. Mg-10Gd induced an increase in production of ECM and a bigger cell size, similar to the effects found with differentiation conditions. An increased metabolic activity was observed in cells cultured under the influence of Mg-10Gd extracts, indicated by an acidic pH during most of the culture period. After 7days of culture on the materials, ATDC5 growth, distribution and ECM synthesis were higher on Mg-10Gd samples, followed by Mg-2Ag and PMg, which was influenced by the homogeneity and composition of the degradation layer. This study confirmed the tolerance of ATDC5 cells to Mg-based materials and a chondrogenic effect of Mg-10Gd. Further studies in vitro and in vivo are necessary to evaluate cell reactions to those materials, as well as the effects on bone growth and the biocompatibility of the alloying system in the body.
authors.affiliation: Helmholtz-Zentrum Geesthacht, Institute of Material Research, Department for Structural Research on Macromolecules, Geesthacht, Germany. Electronic address: adela.martinez@hzg.de.
authors.forename: Adela H
authors.initials: AH
authors.lastname: Martinez Sanchez
authors.affiliation: Helmholtz-Zentrum Geesthacht, Institute of Material Research, Department for Structural Research on Macromolecules, Geesthacht, Germany.
authors.forename: Frank
authors.initials: F
authors.lastname: Feyerabend
authors.affiliation: Helmholtz-Zentrum Geesthacht, Institute of Material Research, Department for Structural Research on Macromolecules, Geesthacht, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Laipple
authors.affiliation: Helmholtz-Zentrum Geesthacht, Institute of Material Research, Department for Structural Research on Macromolecules, Geesthacht, Germany.
authors.forename: Regine
authors.initials: R
authors.lastname: Willumeit-Römer
authors.affiliation: Department of Orthopedics and Orthopedic Surgery, Medical University of Graz, Austria.
authors.forename: Annelie
authors.initials: A
authors.lastname: Weinberg
authors.affiliation: Helmholtz-Zentrum Geesthacht, Institute of Material Research, Department for Structural Research on Macromolecules, Geesthacht, Germany.
authors.forename: Bérengère J C
authors.initials: BJC
authors.lastname: Luthringer
mesh_terms: D000497:Alloys / Q000737:chemistry* / Q000378:metabolism / Q000494:pharmacology; D001672:Biocompatible Materials / Q000737:chemistry* / Q000378:metabolism / Q000633:toxicity; D002454:Cell Differentiation / Q000187:drug effects; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D019902:Chondrocytes / Q000166:cytology / Q000378:metabolism; D020219:Chondrogenesis / Q000187:drug effects; D024042:Collagen Type I / Q000235:genetics / Q000378:metabolism; D050659:Core Binding Factor Alpha 1 Subunit / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression / Q000187:drug effects; D006801:Humans; D006863:Hydrogen-Ion Concentration; D008274:Magnesium / Q000737:chemistry*; D008855:Microscopy, Electron, Scanning; D055758:SOX9 Transcription Factor / Q000235:genetics / Q000378:metabolism; D013052:Spectrometry, X-Ray Emission
chemical_list: D000497:Alloys; D001672:Biocompatible Materials; D024042:Collagen Type I; D050659:Core Binding Factor Alpha 1 Subunit; D055758:SOX9 Transcription Factor; D008274:Magnesium
keywords: Biodegradable materials; Cell differentiation; Chondrogenesis; Magnesium alloys