title: Direct immobilization of antibodies on Zn-doped Fe3O4nanoclusters for detection of pathogenic bacteria.
abstract: Zinc-doped magnetic nanoclusters (Zn-MNCs) were synthesized and used to detect pathogenic bacteria in milk. Hydrothermally synthesized Zn-MNCs exhibited stronger magnetic properties than pure MNCs, which facilitated the magnetic separation from the sample using a permanent magnet. The presence of accessible Zn sites allows the direct immobilization of half-fragmented antibodies over Zn-MNCs through strong ZnS bonds and prevents the tedious multiple steps of molecular functionalization or coating with costly noble metals prior to conjugation with an antibody. After the capture and magnetic separation of Salmonella in milk using the antibody-functionalized Zn-MNCs, the concentration of bacteria was determined with a portable ATP luminometer and the detection limit was found to be 10 CFU/mL.
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Monalisa
authors.initials: M
authors.lastname: Pal
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Sanghee
authors.initials: S
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Donghoon
authors.initials: D
authors.lastname: Kwon
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Jeongin
authors.initials: J
authors.lastname: Hwang
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Hyeonjeong
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea.
authors.forename: Seokyung
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Department of Chemical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, Republic of Korea. Electronic address: jeons@postech.ac.kr.
authors.forename: Sangmin
authors.initials: S
authors.lastname: Jeon
mesh_terms: D000818:Animals; D055910:Antibodies, Immobilized / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis; D005516:Food Microbiology; D057230:Limit of Detection; D008892:Milk / Q000382:microbiology*; D053758:Nanoparticles; D012475:Salmonella / Q000302:isolation & purification*; D015032:Zinc
chemical_list: D055910:Antibodies, Immobilized; D015032:Zinc
keywords: Direct immobilization of antibodies; Sensitive and selective pathogenic bacteria detection; Zn-doped magnetic nanoclusters