title: Alternative new mesenchymal stem cell source exerts tumor tropism through ALCAM and N-cadherin via regulation of microRNA-192 and -218.
abstract: Gliomas are the most common type of malignant primary brain tumors. Some treatments of gliomas exist, but they are rarely curative. Mesenchymal stem cells (MSCs) are emerging as potential modes of targeted cancer therapy owing to their capacity for homing toward tumor sites. It has been proposed that MSCs derived from various sources, such as bone marrow, adipose tissue and umbilical cord blood, can be used as cell-based therapy for brain tumors. Here, MSCs obtained from the synovial fluid of osteoarthritis or rheumatoid arthritis patients were investigated as therapeutic candidates. Specifically, we compared migratory and adhesive abilities, as well as expression levels of related genes and microRNA in bone marrow derived-MSCs (BMMSCs), adipose derived-MSCs (ADMSCs), and synovial fluid derived-MSCs (SFMSCs) after treatment with conditioned medium from gliomas. Migration and adhesion of SFMSCs increased through upregulation of the activated lymphocyte cell adhesion molecule (ALCAM) and N-cadherin by microRNA-192 and -218 downregulation, similar to BMMSCs and ADMSCs. Migratory capacities of all types of MSCs were evaluated in vivo, and SFMSCs migrated intensively toward gliomas. These results suggest that SFMSCs have potential for use in cell-based antitumor therapies.
authors.affiliation: Department of Biology Education, College of Education, Pusan National University, Pusan, 609-735, South Korea.
authors.forename: Ran
authors.initials: R
authors.lastname: Kim
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, The Catholic University of Korea, College of Medicine, Incheon, 403-720, South Korea.
authors.forename: Sang In
authors.initials: SI
authors.lastname: Park
authors.affiliation: Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul, 120-752, South Korea.
authors.forename: Chang Youn
authors.initials: CY
authors.lastname: Lee
authors.affiliation: Department of Biology Education, College of Education, Pusan National University, Pusan, 609-735, South Korea.
authors.forename: Jihyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Department of Biology Education, College of Education, Pusan National University, Pusan, 609-735, South Korea.
authors.forename: Pilseog
authors.initials: P
authors.lastname: Kim
authors.affiliation: Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, 94305, USA.
authors.forename: Sekyung
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Pharmacology, Yale University School of Medicine, New Haven, CT, 06520, USA.
authors.forename: Hojin
authors.initials: H
authors.lastname: Lee
authors.affiliation: Department of Molecular Physiology, College of Pharmacy, Kyungpook National University, Daegu, 702-701, South Korea.
authors.forename: Min Young
authors.initials: MY
authors.lastname: Lee
authors.affiliation: Department of Life Systems, Sookmyung Women's University, 52 Hyochangwon-gil, Seoul, 140-742, South Korea.
authors.forename: Jongmin
authors.initials: J
authors.lastname: Kim
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, The Catholic University of Korea, College of Medicine, Incheon, 403-720, South Korea.
authors.forename: Yong-An
authors.initials: YA
authors.lastname: Chung
authors.affiliation: Institute for Bio-Medical Convergence, College of Medicine, Catholic Kwandong University, Gangwon-do, 210-701, South Korea.|Catholic Kwandong University International, St. Mary's Hospital, Incheon, 404-834, South Korea.
authors.forename: Ki-Chul
authors.initials: KC
authors.lastname: Hwang
authors.affiliation: Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, The Catholic University of Korea, College of Medicine, Incheon, 403-720, South Korea. mls1004@catholic.ac.kr.
authors.forename: Lee-So
authors.initials: LS
authors.lastname: Maeng
authors.affiliation: Department of Biology Education, College of Education, Pusan National University, Pusan, 609-735, South Korea. wchang1975@pusan.ac.kr.
authors.forename: Woochul
authors.initials: W
authors.lastname: Chang
mesh_terms: D015703:Antigens, CD / Q000378:metabolism*; D001932:Brain Neoplasms / Q000378:metabolism / Q000628:therapy; D015820:Cadherins / Q000378:metabolism*; D015816:Cell Adhesion Molecules, Neuronal / Q000378:metabolism*; D045744:Cell Line, Tumor; D002465:Cell Movement; D005260:Female; D005326:Fetal Proteins / Q000378:metabolism*; D005910:Glioma / Q000378:metabolism / Q000628:therapy; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D035683:MicroRNAs / Q000096:biosynthesis*; D020410:Activated-Leukocyte Cell Adhesion Molecule
chemical_list: D015703:Antigens, CD; D015820:Cadherins; D015816:Cell Adhesion Molecules, Neuronal; D005326:Fetal Proteins; D035683:MicroRNAs; C105613:ALCAM protein, human; D020410:Activated-Leukocyte Cell Adhesion Molecule; C050361:CDH2 protein, human; C546783:MIRN192 microRNA, human; C547471:MIRN218 microRNA, human
keywords: Activated lymphocyte cell adhesion molecule; N-Cadherin; Synovial fluid derived-mesenchymal stem cells; Tumor tropism; microRNA-192; microRNA-218