title: Effects of Vitamin D3 on asymmetric- and symmetric dimethylarginine in arterial hypertension.
abstract: Accumulating evidence has proposed a correlation between vitamin D (25(OH)D) insufficiency and cardiovascular (CV) disease. Vitamin D associated effects on endothelial function have been suggested to be a possible culprit. The present study investigated the association of vitamin D3 treatment on markers of endothelial dysfunction in patients with arterial hypertension.
  
  
  
  The Styrian Vitamin D Hypertension Trial is a double-blind, placebo-controlled, single-centre study conducted at the Medical University of Graz, Austria. A total of 200 study participants with arterial hypertension and 25(OH)D levels below 30ng/mL were enrolled. The study participants were randomized to receive 2800 IU of vitamin D3 per day as oily drops (n=100) or placebo (n=100) for a duration of eight weeks. The present study uses an analysis of covariance (ANCOVA) to investigate the effect of vitamin D3 treatment on symmetric (SDMA) and asymmetric dimethylarginine (ADMA). A total of 187 participants (mean [SD] age 60.0 [11.3] years; 47% women; 25(OH)D 21.2 [5.6]ng/mL; mean systolic blood pressure of 131.4 [8.9] mmHg on a median of 2 antihypertensive drugs) completed the trial. Mean treatment effect was -0.004 (95%CI [-0.03 to 0.04]; P=0.819) on ADMA and 0.001 (95%CI [-0.05 to 0.05]; P=0.850) on SDMA. In the subgroup analysis patients with a 25(OH)D concentration <20ng/mL had a significant increase in their log l-arginine/ADMA ratio (mean treatment effect 18.4 95%CI [1.84-34.9]μmol/L/μmol/L; P=0.030). ClinicalTrials.gov Identifier: NCT02136771 EudraCT number: 2009-018125-70 CONCLUSIONS: Vitamin D3 supplementation in hypertensive patients with low 25-hydroxyvitamin D has no significant effect on ADMA and SDMA.
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria; Swiss Cardiovascular Center Bern, Department of Cardiology, Bern University Hospital, University of Bern, 3007 Bern, Switzerland. Electronic address: martin.gruebler@gmx.net.
authors.forename: M R
authors.initials: MR
authors.lastname: Grübler
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria.
authors.forename: M
authors.initials: M
authors.lastname: Gaksch
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria.
authors.forename: K
authors.initials: K
authors.lastname: Kienreich
authors.affiliation: Department of Cardiology, Medical University of Graz, Graz, Austria.
authors.forename: N D
authors.initials: ND
authors.lastname: Verheyen
authors.affiliation: Department of Cardiology, Medical University of Graz, Graz, Austria.
authors.forename: J
authors.initials: J
authors.lastname: Schmid
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Müllner
authors.affiliation: Institute of Experimental and Clinical Pharmacology, Medical University of Graz, Graz, Austria.
authors.forename: G
authors.initials: G
authors.lastname: Richtig
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
authors.forename: H
authors.initials: H
authors.lastname: Scharnagl
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Trummer
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria.
authors.forename: V
authors.initials: V
authors.lastname: Schwetz
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Meinitzer
authors.affiliation: Department of Cardiology, Campus Virchow, Charité University, Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Pieske
authors.affiliation: Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Graz, Graz, Austria; Synlab Academy, Synlab Services GmbH, Mannheim, Germany.
authors.forename: W
authors.initials: W
authors.lastname: März
authors.affiliation: Department of Cardiology, Medical University of Graz, Graz, Austria; Bad Gleichenberg Clinic, Bad Gleichenberg, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Tomaschitz
authors.affiliation: Department of Internal Medicine, Division of Endocrinology and Diabetology, Medical University of Graz, Graz, Austria; Department of Epidemiology and Biostatistics, EMGO Institute for Health and Care Research, VU University Medical Centre, Amsterdam, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Pilz
mesh_terms: D000368:Aged; D000704:Analysis of Variance; D001120:Arginine / Q000031:analogs & derivatives* / Q000097:blood; D001794:Blood Pressure / Q000187:drug effects; D002762:Cholecalciferol / Q000008:administration & dosage*; D019587:Dietary Supplements; D004311:Double-Blind Method; D005260:Female; D006801:Humans; D006973:Hypertension / Q000097:blood* / Q000150:complications / Q000178:diet therapy; D008297:Male; D008875:Middle Aged; D014807:Vitamin D / Q000031:analogs & derivatives* / Q000097:blood; D014808:Vitamin D Deficiency / Q000097:blood* / Q000150:complications / Q000178:diet therapy
chemical_list: D014807:Vitamin D; D002762:Cholecalciferol; C024917:symmetric dimethylarginine; C018524:N,N-dimethylarginine; D001120:Arginine; C104450:25-hydroxyvitamin D
keywords: Arterial hypertension; Cardiovascular disease; Endocrinology; Endothelial dysfunction; Vitamin D; Vitamin D deficiency