title: Divergent Regulation of CBF Regulon on Cold Tolerance and Plant Phenotype in Cassava Overexpressing ArabidopsisCBF3Gene.
abstract: Cassava is a tropical origin plant that is sensitive to chilling stress. In order to understand the CBF cold response pathway, a well-recognized regulatory mechanism in temperate plants, in cassava, overexpression of an ArabidopsisCBF3gene is studied. This gene renders cassava increasingly tolerant to cold and drought stresses but is associated with retarded plant growth, leaf curling, reduced storage root yield, and reduced anthocyanin accumulation in a transcript abundance-dependent manner. Physiological analysis revealed that the transgenic cassava increased proline accumulation, reduced malondialdehyde production, and electrolyte leakage under cold stress. These transgenic lines also showed high relative water content when faced with drought. The expression of partial CBF-targeted genes in response to cold displayed temporal and spatial variations in the wild-type and transgenic plants: highly inducible in leaves and less altered in apical buds. In addition, anthocyanin accumulation was inhibited by downregulating the expression of genes involved in its biosynthesis and by interplaying between theCBF3and the endogenous transcription factors. Thus, the heterologousCBF3modulates the expression of stress-related genes and carries out a series of physiological adjustments under stressful conditions, showing a varied regulation pattern of CBF regulon from that of cassava CBFs.
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science Shanghai, China.
authors.forename: Dong
authors.initials: D
authors.lastname: An
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science Shanghai, China.
authors.forename: Qiuxiang
authors.initials: Q
authors.lastname: Ma
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of ScienceShanghai, China; Institute of Tropical and Sub-tropical Cash Crops, Yunnan Academy of Agricultural SciencesBaoshan, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Yan
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of ScienceShanghai, China; Institute of Tropical and Sub-tropical Cash Crops, Yunnan Academy of Agricultural SciencesBaoshan, China.
authors.forename: Wenzhi
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Institute of Tropical and Sub-tropical Cash Crops, Yunnan Academy of Agricultural Sciences Baoshan, China.
authors.forename: Guanghua
authors.initials: G
authors.lastname: Liu
authors.affiliation: National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Science Shanghai, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhang
keywords: CBF regulon; CBF3; Manihot esulenta Crantz; cold and drought tolerance; phenotype change