title: A common CD55 rs2564978 variant is associated with the susceptibility of non-small cell lung cancer.
abstract: CD55, as one of key membrane-bound complement-regulatory proteins (mCRPs), is crucial for the progression of various cancers. This study aims to investigate the role of CD55 variants in the development of non-small cell lung cancer (NSCLC). A case-control study, including 706 lung cancer cases and 706 health controls, was conducted in a Chinese population. The odds ratio (OR) and 95% confidence interval (95% CI) were estimated by unconditional logistic regression. We found that significantly higher lung cancer risk was linked with CD55 rs2564978 CC genotype (OR = 1.52, 95% CI = 1.11-2.07) or CT genotypes (OR = 1.34, 95% CI = 1.05-1.71), compared to the TT genotype. Stratified analysis showed that rs2564978 CC was associated with NSCLC risk among males (OR = 1.69, 95% CI = 1.14-2.49) and older subjects (OR = 1.75, 95% CI = 1.08-2.82). When stratified by smoking status, the risk effect of rs2564978 CC was more evident among smokers (OR = 2.01, 95% CI = 1.18-3.43) than non-smokers (OR = 1.30, 95% CI = 0.88-1.90). We also conducted the stratified analysis by NSCLC histological types and found that CD55 rs2564978 CC increased the risk of adenocarcinoma with OR (95% CI) of 1.35 (1.01-1.80). The reporter gene expression driven by rs2564978T-containing CD55 promoter was respectively 1.48-fold, 1.96-fold and 1.93-fold higher than those driven by the rs2564978C-containing CD55 promoter in A549, NCI-H2030 and NCI-H23 cells (P = 0.045, 0.010 and < 0.001). These findings indicate that CD55 rs2564978 polymorphism may contribute to an increased risk of NSCLC in Chinese population.
authors.affiliation: Institute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.|Institute of Epidemiology, School of Public Health, North China University of Science and Technology, Tangshan, China.
authors.forename: Yanyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Affiliated Tangshan Gongren Hospital, North China University of Science and Technology, Tangshan, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Cao
authors.affiliation: Institute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
authors.forename: Jia
authors.initials: J
authors.lastname: Lin
authors.affiliation: Institute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
authors.forename: Zhenbang
authors.initials: Z
authors.lastname: Yang
authors.affiliation: Institute of Molecular Genetics, College of Life Science, North China University of Science and Technology, Tangshan, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000072283:A549 Cells; D000368:Aged; D014408:Biomarkers, Tumor / Q000235:genetics* / Q000378:metabolism; D018958:CD55 Antigens / Q000235:genetics* / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D016022:Case-Control Studies; D016009:Chi-Square Distribution; D002681:China; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D005787:Gene Frequency; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D006579:Heterozygote; D006720:Homozygote; D006801:Humans; D016015:Logistic Models; D008175:Lung Neoplasms / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D016017:Odds Ratio; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide; D011401:Promoter Regions, Genetic; D018570:Risk Assessment; D012307:Risk Factors; D012907:Smoking / Q000009:adverse effects; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; D018958:CD55 Antigens
keywords: CD55; complement; lung cancer; single nucleotide polymorphism; susceptibility