title: Quantitative bioanalysis of strontium in human serum by inductively coupled plasma-mass spectrometry.
abstract: OBJECTIVE
  A bioanalytical method using inductively-coupled plasma-mass spectrometry to measure endogenous levels of strontium in human serum was developed and validated.
  This article details the experimental procedures used for the method development and validation thus demonstrating the application of the inductively-coupled plasma-mass spectrometry method for quantification of strontium in human serum samples. The assay was validated for specificity, linearity, accuracy, precision, recovery and stability. Significant endogenous levels of strontium are present in human serum samples ranging from 19 to 96 ng/ml with a mean of 34.6 ± 15.2 ng/ml (SD).
  
  
  CONCLUSIONS
  Calibration procedures and sample pretreatment were simplified for high throughput analysis. The validation demonstrates that the method was sensitive, selective for quantification of strontium (88Sr) and is suitable for routine clinical testing of strontium in human serum samples.
authors.affiliation: Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA; Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA.
authors.forename: Srikanth
authors.initials: S
authors.lastname: Somarouthu
authors.affiliation: Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA; Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA.
authors.forename: Jayoung
authors.initials: J
authors.lastname: Ohh
authors.affiliation: Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA; Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Shaked
authors.affiliation: Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA; Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA.
authors.forename: Robert L
authors.initials: RL
authors.lastname: Cunico
authors.affiliation: Cosmederm BioScience, 8910 University Center Lane #450, San Diego, CA 92122, USA; Cosmederm BioScience, 8910 University Center Lane #450, San Diego, CA 92122, USA.
authors.forename: Gerald
authors.initials: G
authors.lastname: Yakatan
authors.affiliation: Cosmederm BioScience, 8910 University Center Lane #450, San Diego, CA 92122, USA; Cosmederm BioScience, 8910 University Center Lane #450, San Diego, CA 92122, USA.
authors.forename: Suzana
authors.initials: S
authors.lastname: Corritori
authors.affiliation: Therapeutics Inc., 9025 Balboa Ave., Suite 100, San Diego, CA 92123, USA; Therapeutics Inc., 9025 Balboa Ave., Suite 100, San Diego, CA 92123, USA.
authors.forename: Joe
authors.initials: J
authors.lastname: Tami
authors.affiliation: Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA; Pacific BioLabs, 551 Linus Pauling Drive, Hercules, CA 94547, USA.
authors.forename: Erik D
authors.initials: ED
authors.lastname: Foehr
keywords: Yttrium; inductively coupled plasma mass spectrometry; method validation; serum; strontium