title: Adenovirus-vectored foot-and-mouth disease vaccine confers early and full protection against FMDV O1 Manisa in swine.
abstract: A human adenovirus (Ad5) vectored foot-and-mouth disease virus (FMDV) O1-Manisa subunit vaccine (Ad5-O1Man) was engineered to deliver FMDV O1-Manisa capsid and capsid-processing proteins. Swine inoculated with Ad5-O1Man developed an FMDV-specific humoral response as compared to animals inoculated with an empty Ad5-vector. Vaccinated animals were completely protected against homologous challenge at 7 or 21 days post-vaccination. Potency studies exhibited a PD50 of about 107pfu/animal while a dose of 4×107pfu/animal fully protected swine against FMDV intradermal challenge. In-vitro cross-neutralization analysis distinctly predicted that swine vaccinated with Ad5-O1Man would be protected against challenge with homologous FMDV O1Man Middle East-South Asia (ME-SA) topotype and also against recent outbreak strains of Mya-98 South East Asia (SEA) lineage including O1-UK-2001 and O1-South Korea-2010. These results indicate that recombinant Ad5-O1Man is an effective, safe and cross-reacting vaccine that could potentially be used preventively and in outbreak situations, to control FMDV O Mya-98 lineage in swine.
authors.affiliation: Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA; Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA.
authors.forename: Ignacio
authors.initials: I
authors.lastname: Fernandez-Sainz
authors.affiliation: Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA; ORISE-PIADC Research Participation Program, Oak Ridge, TN 37831, USA.
authors.forename: Gisselle N
authors.initials: GN
authors.lastname: Medina
authors.affiliation: Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, CT 06269, USA; ORISE-PIADC Research Participation Program, Oak Ridge, TN 37831, USA.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Ramirez-Medina
authors.affiliation: Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA.
authors.forename: Marla J
authors.initials: MJ
authors.lastname: Koster
authors.affiliation: Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA.
authors.forename: Marvin J
authors.initials: MJ
authors.lastname: Grubman
authors.affiliation: Plum Island Animal Disease Center, ARS, USDA, Greenport, NY 11944, USA. Electronic address: teresa.delossantos@ars.usda.gov.
authors.forename: Teresa
authors.initials: T
authors.lastname: de Los Santos
mesh_terms: D000256:Adenoviridae / Q000235:genetics / Q000378:metabolism; D000257:Adenoviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000914:Antibodies, Viral / Q000276:immunology; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D005536:Foot-and-Mouth Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D029544:Foot-and-Mouth Disease Virus / Q000235:genetics / Q000276:immunology*; D005822:Genetic Vectors / Q000235:genetics / Q000378:metabolism; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D014611:Vaccination; D014765:Viral Vaccines / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology*
chemical_list: D000914:Antibodies, Viral; D036022:Capsid Proteins; D014765:Viral Vaccines
keywords: Ad5-FMD; Adenovirus; Cross-protection; Disease; FMDV; Foot-and-mouth; O1Manisa; Swine; Vaccine