title: Primordial germ cell differentiation of nuclear transfer embryonic stem cells using surface modified electroconductive scaffolds.
abstract: A combination of nanotopographical cues and surface modification of collagen and fibronectin is a potential platform in primordial germ cells (PGCs) differentiation. In the present study, the synergistic effect of nanotopography and surface modification on differentiation of nuclear transfer embryonic stem cells (nt-ESCs) toward PGC lineage was investigated. In order to achieve this goal, poly-anyline (PANi) was mix within poly-L-lactic acid (PLLA). Afterward, the random composite mats were fabricated using PLLA and PANi mix solution. The nanofiber topography notably upregulated the expressions of prdm14, mvh and c-kit compared with tissue culture polystyrene (TCP). Moreover, the combination of nanofiber topography and surface modification resulted in more enhancement of PGCs differentiation compared with non-modified nanofibrous scaffold. Additionally, gene expression results showed that mvh and c-kit were expressed at higher intensity in cells exposed to collagen and fibronectin rather than collagen or fibronectin solitary. These results demonstrated the importance of combined effect of collagen and fibronectin in order to develop a functional extracellular matrix (ECM) mimic in directing stem cell fate and the potential of such biofunctional scaffolds for treatment of infertility.
authors.affiliation: Stem Cells Technology Research Center, Tehran, Iran.
authors.forename: Tarlan
authors.initials: T
authors.lastname: Eslami-Arshaghi
authors.affiliation: Stem Cells Technology Research Center, Tehran, Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Vakilian
authors.affiliation: Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Seyedjafari
authors.affiliation: Stem Cells Technology Research Center, Tehran, Iran.|Department of Tissue Engeneering and Regenerative Medicine, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
authors.affiliation: Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Masoud
authors.initials: M
authors.lastname: Soleimani
authors.affiliation: Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. M.Salehi@sbmu.ac.ir.|Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. M.Salehi@sbmu.ac.ir.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Salehi
mesh_terms: D000814:Aniline Compounds / Q000737:chemistry; D000818:Animals; D002454:Cell Differentiation; D049109:Cell Proliferation; D048430:Cell Shape; D002470:Cell Survival; D004553:Electric Conductivity; D053595:Embryonic Stem Cells / Q000166:cytology* / Q000378:metabolism / Q000648:ultrastructure; D005786:Gene Expression Regulation; D005854:Germ Cells / Q000166:cytology*; D051379:Mice; D057139:Nanofibers / Q000648:ultrastructure; D053652:Nuclear Transfer Techniques; D011091:Polyesters / Q000737:chemistry; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D060888:Real-Time Polymerase Chain Reaction; D017550:Spectroscopy, Fourier Transform Infrared; D013718:Tensile Strength; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D000814:Aniline Compounds; D011091:Polyesters; D012333:RNA, Messenger; C416807:polyaniline; C033616:poly(lactide)
keywords: Collagen; Electroconductive scaffolds; Fibronectin; Nuclear transfer embryonic stem cells; Primordial germ cells