title: The p38 MAPK inhibitor, SB203580, inhibits cell invasion by Neospora caninum.
abstract: The Apicomplexan parasite Neospora caninum is an obligate intracellular parasitic protozoan. It can cause severe diseases in a number of animals throughout the world. Infection with N. caninum leads to abortions in pregnant animals and neuromuscular disorders of newborns which cause great economic losses to animal husbandry. However, the mechanism of cell invasion by N. caninum is still unclear. This paper aims to investigate the impact of SB203580, a p38 MAPK inhibitor, on host cell invasion by N. caninum. The results suggested the presence of putative p38 MAPK homologues in N. caninum, and incubation of N. caninum with SB203580 markedly reduced the tachyzoite motility and microneme exocytosis (NcMIC2, 3, and 6). Furthermore, treatment or pretreatment of MDBK cells with SB203580 effectively reduced cell invasion by N. caninum. Therefore, SB203580 affected both, parasites and host cells, resulting in inhibition of cell invasion by N. caninum.
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Jin
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, China.
authors.forename: Pengtao
authors.initials: P
authors.lastname: Gong
authors.affiliation: Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin, 132101, China.
authors.forename: Guojiang
authors.initials: G
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, China.
authors.forename: Xichen
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Zoonosis, Ministry of Education, College of Veterinary Medicine, Jilin University, 5333 Xian Road, Changchun, 130062, China. Jianhuali7207@163.com.|Jilin Agricultural Science and Technology University, 77 Hanlin Road, Jilin, 132101, China. Jianhuali7207@163.com.
authors.forename: Jianhua
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D002522:Chlorocebus aethiops; D003048:Coccidiosis / Q000188:drug therapy / Q000469:parasitology / Q000662:veterinary*; D004791:Enzyme Inhibitors / Q000494:pharmacology*; D005089:Exocytosis / Q000187:drug effects; D007093:Imidazoles / Q000494:pharmacology*; D051379:Mice; D018688:Neospora / Q000187:drug effects* / Q000502:physiology; D011725:Pyridines / Q000494:pharmacology*; D011817:Rabbits; D014709:Vero Cells; D048051:p38 Mitogen-Activated Protein Kinases / Q000037:antagonists & inhibitors*
chemical_list: D004791:Enzyme Inhibitors; D007093:Imidazoles; D011725:Pyridines; D048051:p38 Mitogen-Activated Protein Kinases; C093642:SB 203580
keywords: Cell invasion; MAPK inhibitor; Neospora caninum; SB203580