title: Comparison of three novel biphasic scaffolds for one-stage treatment of osteochondral defects in a sheep model.
abstract: In the last years, several tissue engineering techniques have been applied to develop different kinds of osteochondral substitutes to overcome the scarce reparative properties of this tissue. The aim of this study was to generate and compare three biphasic scaffolds in an osteochondral lesion in a large-animal model. A critical osteochondral defect was generated in the medial femoral condyle of 18 skeletally mature sheep. Three defects were left untreated, the remaining lesions were divided into three groups: 5 lesions were treated with a biphasic scaffold made of collagen type I and small cylinders of Magnesium Hydroxyapatite; 5 lesions were treated with a biphasic substituted formed by collagen type I and Wollastonite, 5 lesions were treated with a scaffold made of collagen type I and small cylinders of Wollastonite/Hydroxyapatite. Animals were sacrificed after 3 months and samples were analyzed by CT and MRI, macroscopic evaluation and histology. Our study demonstrated that one of these novel biphasic scaffolds possesses the potential for being applied for one-stage procedures for osteochondral defects.
authors.affiliation: Department of Engineering for Innovation, University of Salento, Lecce, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Gervaso
authors.affiliation: IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
authors.forename: L
authors.initials: L
authors.lastname: Mangiavini
authors.affiliation: Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Di Giancamillo
authors.affiliation: Laboratory of Biological Structure Mechanics, Department of Structural Engineering, Politecnico di Milano, Milan, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Boschetti
authors.affiliation: Department of Engineering for Innovation, University of Salento, Lecce, Italy.
authors.forename: D
authors.initials: D
authors.lastname: Izzo
authors.affiliation: Department of Veterinary Medicine (DIMEVET), Ospedale Didattico Universitario Az. Polo Veterinario di Lodi, University of Milan, Milan, Italy.
authors.forename: D D
authors.initials: DD
authors.lastname: Zani
authors.affiliation: Department of Veterinary Medicine (DIMEVET), Ospedale Didattico Universitario Az. Polo Veterinario di Lodi, University of Milan, Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Di Giancamillo
authors.affiliation: IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Tessaro
authors.affiliation: Residency Program in Orthopaedics and Traumatology, University of Milan, Milan, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Domenicucci
authors.affiliation: Department of Engineering for Innovation, University of Salento, Lecce, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Scalera
authors.affiliation: Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy.
authors.forename: C
authors.initials: C
authors.lastname: Domeneghini
authors.affiliation: Dottorato di Ricerca in Sanità e Scienze Sperimentali Veterinarie –DMV, University of Perugia, Perugia, Italy.
authors.forename: A M
authors.initials: AM
authors.lastname: Crovace
authors.affiliation: Department of Engineering for Innovation, University of Salento, Lecce, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Sannino
authors.affiliation: IRCCS Istituto Ortopedico Galeazzi, Milan, Italy.|Department of Biomedical Sciences for Health, University of Milan, Milan, Italy
authors.forename: G M
authors.initials: GM
authors.lastname: Peretti
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000473:pathology* / Q000628:therapy*; D019902:Chondrocytes / Q000473:pathology*; D024042:Collagen Type I / Q000737:chemistry; D004195:Disease Models, Animal; D017886:Durapatite / Q000737:chemistry; D005269:Femur / Q000473:pathology; D010011:Osteocytes / Q000473:pathology*; D012756:Sheep; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*
chemical_list: D024042:Collagen Type I; D017886:Durapatite