title: RF-EMF exposure at 1800 MHz did not elicit DNA damage or abnormal cellular behaviors in different neurogenic cells.
abstract: Despite many years of studies, the debate on genotoxic effects of radiofrequency electromagnetic fields (RF-EMF) continues. To systematically evaluate genotoxicity of RF-EMF, this study examined effects of RF-EMF on DNA damage and cellular behavior in different neurogenic cells. Neurogenic A172, U251, and SH-SY5Y cells were intermittently (5 min on/10 min off) exposed to 1800 MHz RF-EMF at an average specific absorption rate (SAR) of 4.0 W/kg for 1, 6, or 24 h. DNA damage was evaluated by quantification of γH2AX foci, an early marker of DNA double-strand breaks. Cell cycle progression, cell proliferation, and cell viability were examined by flow cytometry, hemocytometer, and cell counting kit-8 assay, respectively. Results showed that exposure to RF-EMF at an SAR of 4.0 W/kg neither significantly induced γH2AX foci formation in A172, U251, or SH-SY5Y cells, nor resulted in abnormal cell cycle progression, cell proliferation, or cell viability. Furthermore, prolonged incubation of these cells for up to 48 h after exposure did not significantly affect cellular behavior. Our data suggest that 1800 MHz RF-EMF exposure at 4.0 W/kg is unlikely to elicit DNA damage or abnormal cellular behaviors in neurogenic cells. Bioelectromagnetics. 38:175-185, 2017. © 2016 Wiley Periodicals, Inc.
authors.affiliation: Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.|Department of Public Health, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Liling
authors.initials: L
authors.lastname: Su
authors.affiliation: Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Wei
authors.affiliation: Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.|Department of Public Health, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Zhengping
authors.initials: Z
authors.lastname: Xu
authors.affiliation: Bioelectromagnetics Laboratory, Zhejiang University School of Medicine, Hangzhou, China.|Department of Public Health, Zhejiang University School of Medicine, Hangzhou, China.
authors.forename: Guangdi
authors.initials: G
authors.lastname: Chen
mesh_terms: D015112:4-Nitroquinoline-1-oxide / Q000633:toxicity; D002453:Cell Cycle; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival; D004249:DNA Damage / Q000187:drug effects; D004574:Electromagnetic Fields / Q000009:adverse effects*; D005909:Glioblastoma / Q000235:genetics / Q000473:pathology*; D006657:Histones / Q000235:genetics; D006801:Humans; D009152:Mutagenicity Tests / Q000295:instrumentation / Q000379:methods; D009447:Neuroblastoma / Q000235:genetics / Q000473:pathology*; D011846:Radio Waves
chemical_list: C468783:H2AX protein, human; D006657:Histones; D015112:4-Nitroquinoline-1-oxide
keywords: DNA damage; cell cycle; cell proliferation; cell viability; mobile phone