title: The MHC Class II Immunopeptidome of Lymph Nodes in Health and in Chemically Induced Colitis.
abstract: We recently described a mass spectrometry-based methodology that enables the confident identification of hundreds of peptides bound to murine MHC class II (MHCII) molecules. In this article, we describe its application to the characterization of MHCII-bound peptides isolated from lymph nodes (LNs) of C57BL/6 mice. More than 1000 peptides could be identified in individual analyses, allowing a direct comparison of the MHCII peptidome in different types of normal LNs or in animals with colitis. The peptide length distribution and consensus sequences in axillary, brachial, inguinal, and mesenteric LNs were virtually identical, and a substantial portion of identified peptides corresponded to proteins found in all LNs. However, skin-specific proteins Sbsn and Dmkn and intestine-specific proteins Dmbt1, Krt19, and Maoa, among others, were exclusively identified in skin-draining and mesenteric LNs, respectively. Differences in peptide-presentation patterns were also observed when comparing healthy mice and mice with dextran sodium sulfate-induced colitis. Peptides derived from a subset of proteins (including IgE, Bank1, chondroitin sulfate synthase 2, Cmip, and Fth1) were exclusively identified in mice with colitis, revealing changes in the peptidome associated with the inflammatory process, as well as activation and clonal expansion of B cells.
authors.affiliation: Philochem AG, CH-8112 Otelfingen, Switzerland; and dario.neri@pharma.ethz.ch tim.fugmann@philochem.ch.
authors.forename: Tim
authors.initials: T
authors.lastname: Fugmann
authors.affiliation: Institute of Pharmaceutical Sciences, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Adriana
authors.initials: A
authors.lastname: Sofron
authors.affiliation: Philochem AG, CH-8112 Otelfingen, Switzerland; and.
authors.forename: Danilo
authors.initials: D
authors.lastname: Ritz
authors.affiliation: Institute of Pharmaceutical Sciences, ETH Zurich, 8093 Zurich, Switzerland.
authors.forename: Franziska
authors.initials: F
authors.lastname: Bootz
authors.affiliation: Institute of Pharmaceutical Sciences, ETH Zurich, 8093 Zurich, Switzerland dario.neri@pharma.ethz.ch tim.fugmann@philochem.ch.
authors.forename: Dario
authors.initials: D
authors.lastname: Neri
mesh_terms: D000818:Animals; D000942:Antigens, Bacterial / Q000032:analysis; D003092:Colitis / Q000276:immunology*; D000949:Histocompatibility Antigens Class II / Q000032:analysis*; D008198:Lymph Nodes / Q000276:immunology*; D013058:Mass Spectrometry; D051379:Mice; D008810:Mice, Inbred C57BL; D010455:Peptides / Q000032:analysis*
chemical_list: D000942:Antigens, Bacterial; D000949:Histocompatibility Antigens Class II; D010455:Peptides