title: Quantifying western blots: none more black.
abstract: Western blotting is among the most common techniques used in molecular biology and a simple way of assessing the presence or absence of a protein. It is also commonly used to compare protein levels in different conditions or in different tissues. This article illustrates some of the easy ways to arrive at a false conclusion when trying to quantify protein levels from western blots.
authors.affiliation: BMC Biology, London, UK. graham.bell@biomedcentral.com.
authors.forename: Graham
authors.initials: G
authors.lastname: Bell
mesh_terms: D015153:Blotting, Western / Q000379:methods* / Q000592:standards; D011506:Proteins / Q000032:analysis
chemical_list: D011506:Proteins