title: Tyramine Hydrochloride Based Label-Free System for Operating Various DNA Logic Gates and a DNA Caliper for Base Number Measurements.
abstract: DNA is believed to be a promising candidate for molecular logic computation, and the fluorogenic/colorimetric substrates of G-quadruplex DNAzyme (G4zyme) are broadly used as label-free output reporters of DNA logic circuits. Herein, for the first time, tyramine-HCl (a fluorogenic substrate of G4zyme) is applied to DNA logic computation and a series of label-free DNA-input logic gates, including elementary AND, OR, and INHIBIT logic gates, as well as a two to one encoder, are constructed. Furthermore, a DNA caliper that can measure the base number of target DNA as low as three bases is also fabricated. This DNA caliper can also perform concatenated AND-AND logic computation to fulfil the requirements of sophisticated logic computing.
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
authors.forename: Daoqing
authors.initials: D
authors.lastname: Fan
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
authors.forename: Xiaoqing
authors.initials: X
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
authors.forename: Shaojun
authors.initials: S
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, Jilin, 130022, P.R. China.|University of Chinese Academy of Sciences, Beijing, 100039, P.R. China.
authors.forename: Erkang
authors.initials: E
authors.lastname: Wang
mesh_terms: D039301:Computers, Molecular; D004247:DNA / Q000737:chemistry* / Q000378:metabolism; D021881:DNA, Catalytic / Q000737:chemistry / Q000378:metabolism; D054856:G-Quadruplexes; D006851:Hydrochloric Acid / Q000737:chemistry*; D008128:Logic; D013050:Spectrometry, Fluorescence; D014439:Tyramine / Q000737:chemistry*
chemical_list: D021881:DNA, Catalytic; D004247:DNA; D006851:Hydrochloric Acid; D014439:Tyramine
keywords: DNA; G-quadruplexes; fluorescence; logic gates; sensors