title: Designing Selectivity in Dirhodium Metallopeptide Catalysts for Protein Modification.
abstract: The ability to chemically alter proteins is important for broad areas of chemical biology, biophysics, and medicine. Chemical catalysts for protein modification, and particularly rhodium(II) conjugates, represent an important new approach to protein modification that develops novel functionalization approaches while shedding light on the development of selective chemistries in complex environments. Here, we elucidate the reaction parameters that allow selective catalysis and even discrimination among highly similar proteins. Furthermore, we show that quantifying modification allows the measurement of competitive ligand affinity, permitting straightforward measurement of protein-peptide interactions and inhibitors thereof. Taken as a whole, rhodium(II) conjugates replicate many features of enzymes in an entirely chemical construct.
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77005, United States.
authors.forename: Samuel C
authors.initials: SC
authors.lastname: Martin
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77005, United States.
authors.forename: Farrukh
authors.initials: F
authors.lastname: Vohidov
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77005, United States.
authors.forename: Haopei
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77005, United States.
authors.forename: Sarah E
authors.initials: SE
authors.lastname: Knudsen
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77005, United States.
authors.forename: Alex A
authors.initials: AA
authors.lastname: Marzec
authors.affiliation: Department of Chemistry, Rice University , Houston, Texas 77005, United States.
authors.forename: Zachary T
authors.identifier: 0000-0002-8681-0789
authors.initials: ZT
authors.lastname: Ball
mesh_terms: D000480:Alkynes / Q000737:chemistry; D000595:Amino Acid Sequence; D001391:Azo Compounds / Q000737:chemistry; D001710:Biotin / Q000737:chemistry; D002384:Catalysis; D006801:Humans; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck) / Q000737:chemistry; D008958:Models, Molecular; D010455:Peptides / Q000737:chemistry*; D011506:Proteins / Q000737:chemistry*; D012238:Rhodium / Q000737:chemistry*; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D018909:src Homology Domains
chemical_list: D000480:Alkynes; D001391:Azo Compounds; D010455:Peptides; D011506:Proteins; D001710:Biotin; D012238:Rhodium; D019860:Lymphocyte Specific Protein Tyrosine Kinase p56(lck)