title: Biological characters of human dermal fibroblasts derived from foreskin of male infertile patients.
abstract: Dermal fibroblasts play a vital role in maintaining skin function. They not only synthesize and secrete extracellular matrix molecules, but also produce a complex mixture of bioactive factors, which both contribute to immune regulation and wound healing. Fibroblasts isolated from skin tissue exhibit wide range of potentials, especially in regenerative medicine. The use of fibroblast cultures for medical purposes requires standardization of cell preparations. To achieve this, we isolated and characterized dermal fibroblasts from human foreskin with a standardized method. The obtained cells grew as typical morphology of fibroblasts, and expressed intermediate filament protein vimentin and nestin. Immunophenotypic analysis indicated that the isolated fibroblasts expressed mesenchymal surface markers CD73, CD90, CD44 and CD105, and were negative for haematopoietic markers CD45 and CD34. Growth kinetics analysis of the cells showed high proliferative properties. Furthermore, cryopreservation had no influence on cell morphology and growth properties. Here, we describe a standardized, repeatable method for isolation of fibroblasts from human foreskin tissues and identify their biological characters according to morphologic, immunohistologic and proliferative criteria, which would be meaningful for future clinical trials and regenerative medicine purposes.
authors.affiliation: Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Fang
authors.affiliation: Department of Anesthesiology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan, 430030, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Ni
authors.affiliation: Department of Obstetrics and Gynecology, Center of Reproductive Medicine, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310009, China.
authors.forename: Yiting
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Wuhan Tongji Reproductive Medicine Hospital, 128 Sanyang Road, Wuhan, 430013, China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Ye
authors.affiliation: Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Shang
authors.affiliation: Zhong Shen Bioscience Inc., Wuhan, China.
authors.forename: Shiliang
authors.initials: S
authors.lastname: Shen
authors.affiliation: Family Planning Research Institute, Center of Reproductive Medicine, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan, 430030, China; Wuhan Tongji Reproductive Medicine Hospital, 128 Sanyang Road, Wuhan, 430013, China. Electronic address: clxiong951@sina.com.
authors.forename: Chengliang
authors.initials: C
authors.lastname: Xiong
mesh_terms: D050156:Adipogenesis / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics*; D019070:Cell Lineage / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D005347:Fibroblasts / Q000378:metabolism* / Q000473:pathology; D052816:Foreskin / Q000254:growth & development* / Q000473:pathology; D018507:Gene Expression Regulation, Developmental; D006801:Humans; D007248:Infertility, Male / Q000378:metabolism* / Q000473:pathology; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology; D064231:Nestin / Q000096:biosynthesis; D014746:Vimentin / Q000096:biosynthesis
chemical_list: D064231:Nestin; D014746:Vimentin
keywords: Cell markers; Fibroblasts; Growth properties; Human foreskin; Vimentin