title: A dual-label time-resolved fluorescence immunoassay (TRFIA) for screening of Coronary atherosclerosis based on simultaneous detection of Lp-PLA2and HsCRP.
abstract: Atherosclerosis is the underlying cause of most coronary events. The conventional method for coronary atherosclerosis detection is morphological examination or coronary arterionyraphy. These methods are complex and time-consuming. In this study a two-step dual-label TRFIA was developed for the simultaneous detection of Lp-PLA2and hsCRP in a single run. The performance of this assay was first evaluated using clinical serum samples, and then compared with commercialized kits. The sensitivity of this assay for Lp-PLA2 detection was 1ng/mL (dynamic range, 0-1000U/L), and the sensitivity for hsCRP detection was 1mg/L (dynamic range, 1-1000mg/L). High correlation coefficients (R) were obtained between the present dual-label TRFIA and commercially available kits(R=0.99 for LP-PLA2 and hsCRP). The present dual-label TRFIA has high sensitivity, specificity, and accuracy in clinical sample analysis. It is a good alternative to the single-label diagnostic methods.
authors.affiliation: Department of Cardiovascular, Shenzhen Chinese Traditional Medical Hospital, Shenzhen 518033, Guangdong, China. Electronic address: 2658165389@qq.com.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: School of Light Industry Engineering, Guizhou Institute of Industry, Guiyang, 550000, China.
authors.forename: Xiaozhu
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Light Industry Engineering, Guizhou Institute of Industry, Guiyang, 550000, China.
authors.forename: Yinfeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of ICU, Shenzhen Chinese Traditional Medical Hospital, Shenzhen 518033, Guangdong, China.
authors.forename: Ruolan
authors.initials: R
authors.lastname: Huang
authors.affiliation: Department of ICU, Shenzhen Chinese Traditional Medical Hospital, Shenzhen 518033, Guangdong, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Wang
authors.affiliation: Guangzhou Youdi Biotechnology Company, Guangzhou 510000, Guangdong, China.
authors.forename: Laiqing
authors.initials: L
authors.lastname: Li
authors.affiliation: Guangzhou Youdi Biotechnology Company, Guangzhou 510000, Guangdong, China.
authors.forename: Cuicui
authors.initials: C
authors.lastname: Chen
authors.affiliation: Department of Cardiovascular, Shenzhen Chinese Traditional Medical Hospital, Shenzhen 518033, Guangdong, China.
authors.forename: Lijun
authors.initials: L
authors.lastname: Ou
authors.affiliation: Department of ICU, Shenzhen Chinese Traditional Medical Hospital, Shenzhen 518033, Guangdong, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chang
authors.affiliation: Department of ICU, Shenzhen Chinese Traditional Medical Hospital, Shenzhen 518033, Guangdong, China.
authors.forename: Qiujie
authors.initials: Q
authors.lastname: Qiao
authors.affiliation: Department of ICU, Shenzhen Chinese Traditional Medical Hospital, Shenzhen 518033, Guangdong, China.
authors.forename: Mingtai
authors.initials: M
authors.lastname: Chen
mesh_terms: D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase / Q000097:blood*; D050197:Atherosclerosis / Q000097:blood* / Q000175:diagnosis*; D015415:Biomarkers; D002097:C-Reactive Protein; D003324:Coronary Artery Disease / Q000097:blood* / Q000175:diagnosis*; D015200:Fluoroimmunoassay / Q000379:methods*; D006801:Humans; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D015415:Biomarkers; D002097:C-Reactive Protein; D043203:1-Alkyl-2-acetylglycerophosphocholine Esterase
keywords: Coronary atherosclerosis; Dual-label TRFIA; LP-PLA2; hsCRP