title: Transcriptome and proteome analyses reveal complex mechanisms of reproductive diapause in the two-spotted spider mite, Tetranychus urticae.
abstract: Although a variety of factors underlying diapause have been identified in arthropods and other organisms, the molecular mechanisms regulating diapause are still largely unknown. Here, to better understand this process, we examined diapause-associated genes in the two-spotted spider mite, Tetranychus urticae, by comparing the transcriptomes and proteomes of early diapausing and reproductive adult females. Amongst genes underlying diapause revealed by the transcriptomic and proteomic data sets, we described the noticeable change in Ca2+-associated genes, including 65 Ca2+-binding protein genes and 23 Ca2+transporter genes, indicating that Ca2+signalling has a substantial role in diapause regulation. Other interesting changes in diapause included up-regulation of (1) glutamate receptors that may be involved in synaptic plasticity changes, (2) genes involved in cytoskeletal reorganization including genes encoding each of the components of thick and thin filaments, tubulin and members of integrin signalling and (3) genes involved in anaerobic energy metabolism, which reflects a shift to anaerobic energy metabolism in early diapausing mites.
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Zhao
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: X-T
authors.initials: XT
authors.lastname: Zhao
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: J-T
authors.initials: JT
authors.lastname: Sun
authors.affiliation: Beijing Genomics Institute-Shenzhen, Shenzhen, China.
authors.forename: L-F
authors.initials: LF
authors.lastname: Zou
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: S-X
authors.initials: SX
authors.lastname: Yang
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: X
authors.initials: X
authors.lastname: Han
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: W-C
authors.initials: WC
authors.lastname: Zhu
authors.affiliation: Beijing Genomics Institute-Shenzhen, Shenzhen, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Yin
authors.affiliation: Department of Entomology, Nanjing Agricultural University, Nanjing, China.
authors.forename: X-Y
authors.initials: XY
authors.lastname: Hong
mesh_terms: D000693:Anaerobiosis; D000818:Animals; D020013:Calcium Signaling; D003599:Cytoskeleton / Q000378:metabolism; D064666:Diapause, Insect; D005260:Female; D020869:Gene Expression Profiling; D018377:Neurotransmitter Agents / Q000378:metabolism; D020543:Proteome; D012098:Reproduction; D017423:Sequence Analysis, RNA; D040102:Tetranychidae / Q000502:physiology*; D059467:Transcriptome
chemical_list: D018377:Neurotransmitter Agents; D020543:Proteome
keywords: Ca2+ signalling; Tetranychus urticae; diapause; proteome; transcriptome