title: Comparative transcriptomic analysis reveals phenol tolerance mechanism of evolved Chlorella strain.
abstract: The growth of microalgae is inhibited by high concentration phenol due to reactive oxygen species. An evolved strain tolerated to 500mg/L phenol, Chlorella sp. L5, was obtained in previous study. In this study, comparative transcriptomic analysis was performed for Chlorella sp. L5 and its original strain (Chlorella sp. L3). The tolerance mechanism of Chlorella sp. L5 for high concentration phenol was explored on genome scale. It was identified that the up-regulations of the related genes according to antioxidant enzymes (SOD, APX, CAT and GR) and carotenoids (astaxanthin, lutein and lycopene) biosynthesis had critical roles to tolerate high concentration phenol. In addition, most of genes of PS I, PS II, photosynthetic electron transport chain and starch biosynthesis were also up-regulated. It was consistent to the experimental results of total carbohydrate contents of Chlorella sp. L3 and Chlorella sp. L5 under 0mg/L and 500mg/L phenol.
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; School of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; University of Chinese Academy of Sciences, 19 Yuquan Road, Beijing 100049, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China.
authors.forename: Dujia
authors.initials: D
authors.lastname: Cheng
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Life Science, Shanghai University, 99 Shangda Road, Shanghai 200444, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Gao
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China. Electronic address: zhaoqy@sari.ac.cn.
authors.forename: Quanyu
authors.initials: Q
authors.lastname: Zhao
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Shanghai Advanced Research Institute, Chinese Academy of Sciences, 99 Haike Road, Shanghai 201210, China; ShanghaiTech University, 100 Haike Road, Shanghai 201210, China.
authors.forename: Yuhan
authors.initials: Y
authors.lastname: Sun
mesh_terms: D002708:Chlorella / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D004361:Drug Tolerance / Q000235:genetics; D019800:Phenol / Q000633:toxicity*; D059467:Transcriptome
chemical_list: D019800:Phenol
keywords: Antioxidant; Carotenoids; Chlorella; Phenol tolerance; Transcriptomics