title: Regulatory Role ofOsMADS34in the Determination of Glumes Fate, Grain Yield, and Quality in Rice.
abstract: Grasses produce seeds on spikelets, a unique type of inflorescence. Despite the importance of grass crops for food, the genetic mechanisms that control spikelet development remain poorly understood. In this study, we usedm34-z, a new mutant allele of the rice (Oryza sativa) E-class geneOsMADS34, to examine OsMADS34 function in determining the identities of glumes (rudimentary glume and sterile lemma) and grain size. In them34-zmutant, both the rudimentary glume and sterile lemma were homeotically converted to the lemma-like organ and acquired the lemma identity, suggesting that OsMADS34 plays important roles in the development of glumes. In them34-zmutant, most of the grains from the secondary panicle branches (spb) were decreased in size, compared with grains from wild-type, but no differences were observed in the grains from the primary panicle branches. The amylose content and gel consistency, and a seed-setting rate from the spb were reduced in them34-zmutant. Interesting, transcriptional activity analysis revealed that OsMADS34 protein was a transcription repressor and it may influence grain yield by suppressing the expressions ofBG1,GW8,GW2, andGL7in them34-zmutant. These findings revealed thatOsMADS34largely affects grain yield by affecting the size of grains from the secondary branches.
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Deyong
authors.initials: D
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research InstituteZhejiang, China; College of Chemistry and Life Sciences, Zhejiang Normal UniversityZhejiang, China.
authors.forename: Yuchun
authors.initials: Y
authors.lastname: Rao
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Yujia
authors.initials: Y
authors.lastname: Leng
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research InstituteZhejiang, China; College of Life and Environmental Sciences, Hangzhou Normal UniversityZhejiang, China.
authors.forename: Zizhuang
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Qiankun
authors.initials: Q
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Liwen
authors.initials: L
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Zhennan
authors.initials: Z
authors.lastname: Qiu
authors.affiliation: College of Life and Environmental Sciences, Hangzhou Normal University Zhejiang, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Xue
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Dali
authors.initials: D
authors.lastname: Zeng
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Guangheng
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Guojun
authors.initials: G
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Longbiao
authors.initials: L
authors.lastname: Guo
authors.affiliation: State Key Laboratory of Rice Biology, China National Rice Research Institute Zhejiang, China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Qian
keywords: OsMADS34; grain size; rice (Oryza sativa L.); rudimentary glume; spikelet; sterile lemma; transcriptional repressor