title: Differential expression of visfatin, leptin, stromal cell derived factor-1α, endothelial nitric oxide synthase, and vascular endothelial growth factor in human leiomyomas.
abstract: OBJECTIVE
  This study was aimed to understand expressions of the visfatin, leptin, stromal cell derived factor (SDF)-1α, endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor (VEGF) in human uterine leiomyomas (UL) and normal myometrium.
  
  
  METHODS
  This study investigated expression of visfatin, leptin, SDF-1α, eNOS and VEGF in 23 uterine leiomyoma patients and 10 normal myometrium by RT-PCR and western blot. Messenger RNA transcripts of SDF-1α, eNOS, VEGF and hypoxia inducible factor-1α (HIF-1α) were analyzed according to the size of UL by real-time PCR.
  
  
  RESULTS
  There were no significant differences in expressions of visfatin and leptin between UL compared with normal myometrium. However, expressions of eNOS, SDF-1α and VEGF were significantly higher in both intramural and subserosal UL compared with normal myometrium. The expression of SDF1-α was significantly increased in small UL (<5 cm) compared to the large UL (≥5 cm), whereas the expressions of eNOS, VEGF and HIF-1α were higher in large UL than small UL.
  
  
  CONCLUSIONS
  This study shows that expression of SDF-1α, eNOS and VEGF were significantly higher in UL than myometrium with a different expression pattern according to the size of UL. However, expressions of visfatin and leptin had no significant differences between the two groups.
authors.affiliation: a The Korea Institute for Public Sperm Bank , Busan , South Korea.
authors.forename: Bo Sun
authors.initials: BS
authors.lastname: Joo
authors.affiliation: a The Korea Institute for Public Sperm Bank , Busan , South Korea.
authors.forename: Min Jung
authors.initials: MJ
authors.lastname: Park
authors.affiliation: b Department of Obstetrics and Gynecology , Samsung Changwon Hospital, Sungkyunkwan University School of Medicine , Changwon , South Korea , and.
authors.forename: Chang-Woon
authors.initials: CW
authors.lastname: Kim
authors.affiliation: c Department of Obstetrics and Gynecology , Medical Research Institute, Pusan National University School of Medicine , Busan , South Korea.
authors.forename: Kyu Sup
authors.initials: KS
authors.lastname: Lee
authors.affiliation: c Department of Obstetrics and Gynecology , Medical Research Institute, Pusan National University School of Medicine , Busan , South Korea.
authors.forename: Jong Kil
authors.initials: JK
authors.lastname: Joo
mesh_terms: D000328:Adult; D054377:Chemokine CXCL12 / Q000378:metabolism*; D005260:Female; D006801:Humans; D007889:Leiomyoma / Q000378:metabolism* / Q000473:pathology; D020738:Leptin / Q000378:metabolism*; D008875:Middle Aged; D009215:Myometrium / Q000378:metabolism* / Q000473:pathology; D054409:Nicotinamide Phosphoribosyltransferase / Q000378:metabolism*; D052250:Nitric Oxide Synthase Type III / Q000378:metabolism*; D014594:Uterine Neoplasms / Q000378:metabolism* / Q000473:pathology; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism*
chemical_list: D054377:Chemokine CXCL12; D020738:Leptin; D042461:Vascular Endothelial Growth Factor A; D052250:Nitric Oxide Synthase Type III; D054409:Nicotinamide Phosphoribosyltransferase
keywords: SDF-1α; eNOS; letpin; uterine leiomyomas; visfatin