title: Bio-oil extraction of Jatropha curcas with ionic liquid co-solvent: Fate of biomass protein.
abstract: The fate of oil-seed biomass protein has been tracked through all steps of a multi-phase extraction process using an ionic liquid based co-solvent system previously demonstrated to extract bio-oil and phorbol esters and to recover fermentable sugars from Jatropha oil seed. These analyses, however, did not address the fate of biomass protein. This work demonstrated that the majority of protein (∼86%) tracked with the biomass with the balance lost to co-solvent (∼12%) and methanol (∼2%) washes. A significant portion of the ionic liquid remained with the treated biomass and required aggressive methanol washes to recover. A system analysis showed a net-positive energy balance and thus the potential of this system to produce both bio-oil and protein-rich toxin-free biomass. While these results further support Jatropha as an oil seed crop, the additional costs of solvent recovery will need to be addressed if commercialization is to be realized.
authors.affiliation: Hawaii Natural Energy Institute, University of Hawaii at Manoa, 1680 East West Road, Post 109, Honolulu, HI 96822, United States.
authors.forename: Godwin
authors.initials: G
authors.lastname: Severa
authors.affiliation: Hawaii Natural Energy Institute, University of Hawaii at Manoa, 1680 East West Road, Post 109, Honolulu, HI 96822, United States.
authors.forename: Melisa
authors.initials: M
authors.lastname: Edwards
authors.affiliation: Hawaii Natural Energy Institute, University of Hawaii at Manoa, 1680 East West Road, Post 109, Honolulu, HI 96822, United States. Electronic address: mcooney@hawaii.edu.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Cooney
mesh_terms: D018533:Biomass; D005285:Fermentation; D052578:Ionic Liquids / Q000737:chemistry*; D007477:Ions; D031286:Jatropha / Q000737:chemistry*; D008055:Lipids / Q000737:chemistry; D000432:Methanol / Q000737:chemistry; D009584:Nitrogen / Q000032:analysis; D010575:Pesticides / Q000737:chemistry; D010703:Phorbol Esters / Q000737:chemistry; D010940:Plant Proteins / Q000737:chemistry*; D012639:Seeds / Q000737:chemistry; D012997:Solvents / Q000737:chemistry*; D013053:Spectrophotometry
chemical_list: D052578:Ionic Liquids; D007477:Ions; D008055:Lipids; D010575:Pesticides; D010703:Phorbol Esters; D010940:Plant Proteins; D012997:Solvents; D009584:Nitrogen; D000432:Methanol
keywords: Bio-oil bearing biomass; Ionic liquid co-solvents; Jatropha; Protein extraction