title: Assignment of Absolute Configuration of a New Hepatoprotective Schiartane-Type Nortriterpenoid Using X-Ray Diffraction.
abstract: A new schiartane-type nortriterpenoid, micrandilactone H was isolated fromKadsuralongipedunculataFinet et Gagnep. Its 2D (two dimension) structure was elucidated by NMR spectroscopic analysis, and it is similar to that of Kadnanolactones H and the absolute configuration was established through X-ray diffraction and ECD data analysis. This represents the first complete assignment of the absolute configuration of a schiartane-type nortriterpenoid by X-ray diffraction and the ECD method. Micrandilactone H showed moderate hepatoprotective activity againstN-acetyl-p-aminophenol (APAP)-induced toxicity in HepG2 cells with cell survival rates of 56.84% at 10 μM.
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. xiaojuan2013@outlook.com.|Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, USA. xiaojuan2013@outlook.com.
authors.forename: Xiaojuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Louisiana State University, Baton Rouge, LA 70803, USA. ffroncz@lsu.edu.
authors.forename: Frank R
authors.initials: FR
authors.lastname: Fronczek
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. cjb427@hotmail.com.
authors.forename: Jiabao
authors.initials: J
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. ljb_5021@hotmail.com.
authors.forename: Jiabao
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of BioMolecular Sciences, Division of Pharmacognosy, and Research Institute of Pharmaceutical Sciences, School of Pharmacy, University of Mississippi, University, Mississippi 38677-1848, USA. dferreir@olemiss.edu.
authors.forename: Daneel
authors.initials: D
authors.lastname: Ferreira
authors.affiliation: State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China. lishuai@imm.ac.cn.
authors.forename: Shuai
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC 29425, USA. hamannm@musc.edu.
authors.forename: Mark T
authors.initials: MT
authors.lastname: Hamann
mesh_terms: D000082:Acetaminophen / Q000633:toxicity; D017209:Apoptosis / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D056945:Hep G2 Cells; D006801:Humans; D032135:Kadsura / Q000737:chemistry*; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology; D014315:Triterpenes / Q000737:chemistry* / Q000494:pharmacology*; D014961:X-Ray Diffraction
chemical_list: D010936:Plant Extracts; D014315:Triterpenes; C512130:schiartane; D000082:Acetaminophen
keywords: X-ray; absolute configuration; hepatoprotective; schiartane-type nortriterpenoid