title: Comparison of Catabolic Rates of sn-1, sn-2, and sn-3 Fatty Acids in Triacylglycerols Using13CO2Breath Test in Mice.
abstract: Fatty acids in triacylglycerols (TAGs) are catabolized after digestion. However, the catabolic rates of the fatty acids at the sn-1, sn-2, and sn-3 positions of TAGs have not been compared. To elucidate the differences, we studied the catabolic rates of13C-labeled palmitic acid, oleic acid, and capric acid at the sn-1, sn-2, or sn-3 position of TAGs using isotope-ratio mass spectrometry. Specifically, we measured the13C-to-12C ratio in CO2(Δ13C (‰)) exhaled by mice. For all analyzed fatty acids, we observed significant differences between sn-2 and other binding positions. In contrast, no significant difference was detected between the sn-1 and sn-3 positions. These results indicated that the catabolic rates of fatty acids are strongly influenced by their positions in TAGs.
authors.affiliation: Department of Food Science and Technology, Tokyo University of Marine Science and Technology.
authors.forename: Fumiaki
authors.initials: F
authors.lastname: Beppu
authors.forename: Takashi
authors.initials: T
authors.lastname: Kawamatsu
authors.forename: Yoshio
authors.initials: Y
authors.lastname: Yamatani
authors.forename: Toshiharu
authors.initials: T
authors.lastname: Nagai
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Yoshinaga
authors.forename: Hoyo
authors.initials: H
authors.lastname: Mizobe
authors.forename: Akihiko
authors.initials: A
authors.lastname: Yoshida
authors.forename: Atsushi
authors.initials: A
authors.lastname: Kubo
authors.forename: Jota
authors.initials: J
authors.lastname: Kanda
authors.forename: Naohiro
authors.initials: N
authors.lastname: Gotoh
mesh_terms: D000818:Animals; D001944:Breath Tests; D002245:Carbon Dioxide / Q000032:analysis; D002250:Carbon Radioisotopes; D003652:Decanoic Acids / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D013058:Mass Spectrometry; D051379:Mice; D015394:Molecular Structure; D019301:Oleic Acid / Q000737:chemistry / Q000378:metabolism*; D019308:Palmitic Acid / Q000737:chemistry / Q000378:metabolism*; D014280:Triglycerides / Q000737:chemistry / Q000378:metabolism*
chemical_list: D002250:Carbon Radioisotopes; D003652:Decanoic Acids; D014280:Triglycerides; D002245:Carbon Dioxide; D019301:Oleic Acid; D019308:Palmitic Acid; C031071:decanoic acid