title: Simultaneous microbial reduction of vanadium (V) and chromium (VI) by Shewanella loihica PV-4.
abstract: Toxic vanadium (V) and chromium (VI) often co-exist in wastewater from vanadium ore smelting and their reductions by bacterial strain Shewanella loihica PV-4 is realized simultaneously. After 27-d operation, 71.3% of V(V) and 91.2% of Cr(VI) were removed respectively, with citrate as organic carbon source. Enhancement of Cr(VI) bioreduction was observed with the suppressed V(V) reduction. V(IV) and Cr(III), the main reduction products, precipitated inside the organisms and attached on cell surfaces. Both membrane components containing cytochrome c and cytoplasmic fractions containing soluble proteins as well as NADH may contribute to these microbial reductions. Most Cr(VI) were reduced extracellularly and V(V) tended to be reduced through intracellular process, as revealed by mapping the microbial surface and a line scan across the cell, performed by scanning transmission electron microscopy. This study provides an efficient alternative for controlling combined pollution caused by these two metals based on microbial technology.
authors.affiliation: Department of Biophysics, College of Basic Medical Sciences, Peking University Health Science Centre, Beijing 100191, China.
authors.forename: Guangyu
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Water Resources and Environment, China University of Geosciences Beijing, Key Laboratory of Groundwater Circulation and Evolution (China University of Geosciences Beijing), Ministry of Education, Beijing 100083, China.
authors.forename: Baogang
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Biophysics, College of Basic Medical Sciences, Peking University Health Science Centre, Beijing 100191, China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Li
authors.affiliation: School of Water Resources and Environment, China University of Geosciences Beijing, Key Laboratory of Groundwater Circulation and Evolution (China University of Geosciences Beijing), Ministry of Education, Beijing 100083, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Yang
authors.affiliation: Department of Biophysics, College of Basic Medical Sciences, Peking University Health Science Centre, Beijing 100191, China. Electronic address: ccyin@hsc.pku.edu.cn.
authors.forename: Changcheng
authors.initials: C
authors.lastname: Yin
mesh_terms: D002857:Chromium / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction; D020592:Shewanella / Q000378:metabolism*; D014639:Vanadium / Q000737:chemistry / Q000378:metabolism*; D062065:Wastewater / Q000737:chemistry* / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism; D018508:Water Purification / Q000379:methods*
chemical_list: D002857:Chromium; D014639:Vanadium; D062065:Wastewater; D014874:Water Pollutants, Chemical
keywords: Chromium (VI); Microbial reduction; Shewanella loihica PV-4; Vanadium (V)