title: Chemoselective and site-selective peptide and native protein modification enabled by aldehyde auto-oxidation.
abstract: We report a chemoselective and site-selective formylation of ε-amine in native proteins. The aldehyde auto-oxidation re-routing, regulated generation of formate, and reversible N-terminus protection drive the transformation. It labels a single ε-amine in a pool of its copies, other nucleophilic residues, and α-amine. The extension of the methodology leads to site-selective acylation.
authors.affiliation: Organic and Bioconjugate Chemistry Laboratory, Department of Chemistry, Indian Institute of Science Education and Research Bhopal, Bhauri, Bhopal Bypass Road, Bhopal 462066, India. vrai@iiserb.ac.in.
authors.forename: Landa
authors.initials: L
authors.lastname: Purushottam
authors.forename: Srinivasa Rao
authors.initials: SR
authors.lastname: Adusumalli
authors.forename: Maheshwerreddy
authors.initials: M
authors.lastname: Chilamari
authors.forename: Vishal
authors.initials: V
authors.lastname: Rai