title: Thiol peroxiredoxin, a novel allergen fromBombyx mori, modulates functions of macrophages and dendritic cells.
abstract: Bombyx mori(B.mori, also known as silkworm) plays a role in the pathogenesis of allergic diseases. However, its allergens are to be characterized. The aim of this paper is to identify new silkworm allergens. Two-dimensional gel electrophoresis (2-DE) and mass spectrometry were employed to separate and identify potential allergens from silkworm pupa. Six potential allergens were identified in this study. Thiol peroxiredoxin (TP), one of the 6 allergens, reacted to serum IgE from patients sensitized to silkworm. By sensitizing with TP allergic asthma like symptoms were induced in mice, including elevation of the levels of serum IgE, IL-4 from bronchoalveolar lavage fluid and culture supernatant of spleen cells. In vitro experiments showed that TP significantly induced RAW264.7 cells (a macrophage cell line) apoptosis via modulating the BCL2 and Caspase9 pathways. The levels of CD80, CD40, CD83 and TNF-α in DC2.4 cells (a dendritic cell line) were increased in the culture after exposure to TP. In summary, TP is an allergic component of silkworm. It induces allergic asthma, and modulates the functions of macrophages and dendritic cells.
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, School of Medicine, Shenzhen University3688 Nanhai Blvd, Shenzhen 518060, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University Shenzhen 518060, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Hu
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University Shenzhen 518060, China.
authors.forename: Zhiling
authors.initials: Z
authors.lastname: Liang
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, School of Medicine, Shenzhen University3688 Nanhai Blvd, Shenzhen 518060, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University Shenzhen 518060, China.
authors.forename: Jianjie
authors.initials: J
authors.lastname: Li
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen University Shenzhen 518060, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Yan
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, School of Medicine, Shenzhen University3688 Nanhai Blvd, Shenzhen 518060, China.
authors.forename: Pingchang
authors.initials: P
authors.lastname: Yang
authors.affiliation: Otolaryngology Institutes, Allergy and Immunology Research Center, Shenzhen UniversityShenzhen 518060, China; The State Key Laboratory of Respiratory Disease for Allergy at Shenzhen University, School of Medicine, Shenzhen University3688 Nanhai Blvd, Shenzhen 518060, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Departments of Allergy, Peking Union Medical College Hospital Beijing 100730, China.
authors.forename: Lianglu
authors.initials: L
authors.lastname: Wang
keywords: Bombyx mori; allergy; dendritic cells; macrophages; thiol peroxiredoxin