title: Elemental Zinc Is Inversely Associated with C-Reactive Protein and Oxidative Stress in Chronic Liver Disease.
abstract: Chronic liver disease (CLD) is associated with the destruction of liver parenchyma cell. It is the main cause of morbidity and mortality in most of the developed countries. Oxidative stress and altered levels of different trace elements in serum have been documented for different diseases including inflammation and many liver diseases. This study aims to evaluate the serum level of malondialdehyde (MDA), nitric oxide (NO), antioxidant vitamin C, C-reactive protein (CRP), and zinc (Zn) in CLD patients and to establish a correlation among the study parameters with the severity of inflammatory conditions of CLD. In this study, CLD patients and healthy volunteers were recruited. Total cholesterol and triglyceride were determined by colorimeter using enzymatic method. Serum non-enzymatic antioxidant vitamin C, reactive oxygen species nitric oxide (NO), and malondialdehyde (MDA) were determined by UV-spectrophotometric method. Trace element (Zn) levels were determined by graphite furnace atomic absorption spectroscopy. Independent sample t test and Pearson's correlation test were performed for statistical analysis using the statistical software package SPSS, Version 20. Studies showed that the MDA (p < 0.001), NO (p < 0.001), and CRP levels were significantly higher in CLD patients than in control subjects. The antioxidant vitamin C (p < 0.001) and trace element zinc (p < 0.001) were comparatively lower in the CLD patients than in control subjects. Elemental Zn showed an inverse relationship with MDA, NO, and CRP but positively correlated with antioxidant capacity, whereas MDA showed a positive correlation with CRP level. Thus, we conclude that attenuated level of Zn and antioxidant in serum play an important role in the inflammatory status of CLD patients by elevating the concentration of MDA, NO, and CRP.
authors.affiliation: Department of Pharmacy, Noakhali Science and Technology University, Noakhali, -3814, Bangladesh.
authors.forename: Md Giash
authors.initials: MG
authors.lastname: Uddin
authors.affiliation: Department of Pharmacy, Noakhali Science and Technology University, Noakhali, -3814, Bangladesh. pharmasalim@yahoo.com.
authors.forename: Mohammad Salim
authors.initials: MS
authors.lastname: Hossain
authors.affiliation: Department of Pharmacy, Noakhali Science and Technology University, Noakhali, -3814, Bangladesh.
authors.forename: Md Atiqur
authors.initials: MA
authors.lastname: Rahman
authors.affiliation: Department of Pharmacy, Noakhali Science and Technology University, Noakhali, -3814, Bangladesh.
authors.forename: A H M Mazbah
authors.initials: AHMM
authors.lastname: Uddin
authors.affiliation: Department of Pharmacy, Noakhali Science and Technology University, Noakhali, -3814, Bangladesh.
authors.forename: Md Shafiullah
authors.initials: MS
authors.lastname: Bhuiyan
mesh_terms: D000328:Adult; D000368:Aged; D002097:C-Reactive Protein / Q000378:metabolism*; D002908:Chronic Disease; D003430:Cross-Sectional Studies; D006801:Humans; D008107:Liver Diseases / Q000097:blood*; D008875:Middle Aged; D018384:Oxidative Stress; D015032:Zinc / Q000097:blood*
chemical_list: D002097:C-Reactive Protein; D015032:Zinc
keywords: C-reactive protein; Chronic liver disease; Inflammation; Malondialdehyde; Zinc