title: Astragalin Inhibits Allergic Inflammation and Airway Thickening in Ovalbumin-Challenged Mice.
abstract: Lung inflammation and oxidative stress are the major contributors to the development of obstructive pulmonary diseases. Macrophages are involved in pulmonary inflammation and alveolar damage in emphysema. Astragalin is an anti-inflammatory flavonoid present in persimmon leaves and green tea seeds. This study elucidated that astragalin inhibited inflammatory cell infiltration induced by 20 μM H2O2and blocked airway thickening and alveolar emphysema induced by 20 μg of ovalbumin (OVA) in mice. OVA induced mouse pulmonary MCP-1, and H2O2enhanced the expression of MCP-1/ICAM-1/αv integrin in bronchial airway epithelial BEAS-2B cells. Such induction was inhibited by supplying 10-20 mg/kg of astragalin to OVA-challenged mice and 1-20 μM astragalin to oxidant-stimulated cells. Oral administration of 20 mg/kg of astragalin reduced the induction of F4/80/CD68/CD11b in airways of mice challenged with OVA. Additionally, emphysema tissue damage was observed in OVA-exposed alveoli. Mast cell recruitment in the airway subepithelium was blocked by supplementing astragalin to OVA-challenged mice. Orally treating 20 mg/kg of astragalin reduced α-SMA induction in inflammation-occurring airways and appeared to reverse airway thickening and constriction induced by an OVA episode. These results revealed that astragalin may improve airway thickening and alveolar destruction with blockade of allergic inflammation in airways. Therefore, astragalin may be a therapeutic agent antagonizing asthma and obstructive pulmonary diseases.
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Yun-Ho
authors.initials: YH
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Yean-Jung
authors.initials: YJ
authors.lastname: Choi
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Min-Kyung
authors.initials: MK
authors.lastname: Kang
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Sin-Hye
authors.initials: SH
authors.lastname: Park
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Lucia Dwi
authors.initials: LD
authors.lastname: Antika
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Eun-Jung
authors.initials: EJ
authors.lastname: Lee
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Dong Yeon
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Food Science and Nutrition, Hallym University , Chuncheon 24252, Korea.
authors.forename: Young-Hee
authors.identifier: http://orcid.org/0000-0003-2039-7968
authors.initials: YH
authors.lastname: Kang
mesh_terms: D000818:Animals; D018932:Chemokine CCL2 / Q000235:genetics / Q000276:immunology; D004195:Disease Models, Animal; D006801:Humans; D018799:Intercellular Adhesion Molecule-1 / Q000235:genetics / Q000276:immunology; D044949:Kaempferols / Q000008:administration & dosage*; D008173:Lung Diseases, Obstructive / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology*; D008264:Macrophages / Q000187:drug effects / Q000276:immunology; D008297:Male; D008407:Mast Cells / Q000187:drug effects / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D010047:Ovalbumin / Q000009:adverse effects
chemical_list: C491768:Ccl2 protein, mouse; D018932:Chemokine CCL2; D044949:Kaempferols; D018799:Intercellular Adhesion Molecule-1; D010047:Ovalbumin; C001579:astragalin
keywords: airway inflammation; airway smooth muscle; astragalin; macrophages; mast cells; neutrophils; oxidative stress