title: Insulin-like Growth Factor Binding Protein6 Associated with Neuronal Apoptosis Following Intracerebral Hemorrhage in Rats.
abstract: The insulin-like growth factor (IGF) system is linked to CNS pathological states. The functions of IGFs are modulated by a family of binding proteins termed insulin-like growth factor binding proteins (IGFBPs). Here, we demonstrate that IGFBP-6 may be associated with neuronal apoptosis in the processes of intracerebral hemorrhage (ICH). We obtained a significant upregulation of IGFBP-6 in neurons adjacent to the hematoma following ICH with the results of Western blot, immunohistochemistry, and immunofluorescence. Increasing IGFBP-6 level was found to be accompanied by the upregulation of Bax, Bcl-2, and active caspase-3. Besides, IGFBP-6 co-localized well with active caspase-3 in neurons, indicating its potential role in neuronal apoptosis. Knocking down IGFBP-6 by RNA-interference in PC12 cells reduced active caspase-3 expression. Thus, IGFBP-6 may play a role in promoting the brain secondary damage following ICH.
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Qijun
authors.initials: Q
authors.lastname: Dai
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Peipei
authors.initials: P
authors.lastname: Jiang
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gu
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhu
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Haifeng
authors.initials: H
authors.lastname: Dai
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yao
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liu
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Ma
authors.affiliation: Jiangsu Province Key Laboratory for Inflammation and Molecular Drug Target, Medical College of Nantong University, Nantong, 226001, Jiangsu Province, China. nt5621@126.com.
authors.forename: Chengwei
authors.initials: C
authors.lastname: Duan
authors.affiliation: Department of Orthopedics, Traditional Chinese Medicine Hospital of Haian County Affiliated Hospital, Nanjing University of Chinese Medicine of Hanlin College, Ninghai Road, Haian County, 226600, Jiangsu Province, China. qulianxiant@outlook.com.
authors.forename: Lianxia
authors.initials: L
authors.lastname: Qu
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000502:physiology*; D002543:Cerebral Hemorrhage / Q000378:metabolism* / Q000473:pathology; D018976:Insulin-Like Growth Factor Binding Protein 6 / Q000378:metabolism*; D008297:Male; D009474:Neurons / Q000378:metabolism* / Q000473:pathology; D016716:PC12 Cells; D051381:Rats; D017207:Rats, Sprague-Dawley
chemical_list: D018976:Insulin-Like Growth Factor Binding Protein 6
keywords: IGF; IGFBP-6; Intracerebral hemorrhage; Neuronal apoptosis; Rat