title: [Translation elongation factor EF1α1 interacts with ZAD domains of transcription factors from Drosophila melanogaster].
abstract: A large class of arthropod transcription factors is formed by proteins with a characteristic N-terminal Zinc-finger-Associated Domain (ZAD) which contains four cysteine residues that coordinate a zinc ion. The number of putative proteins with ZAD in the Drosophila genome exceeds 90, and the degree of sequence similarity between these domains can be as low as 23%. Efficient binding of ZADs from the proteins Grau, ZIPIC, and Zw5 to the translation elongation factor EF1α1 in nuclear and cytoplasmic extracts has been demonstrated. EF1α1 is probably involved in the regulation of the activity of ZAD-containing transcription factors.
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Zolotarev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: O G
authors.initials: OG
authors.lastname: Maksimenko
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: Yu V
authors.initials: YV
authors.lastname: Shidlovskii
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: P G
authors.initials: PG
authors.lastname: Georgiev
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.|errinaceus@rambler.com.
authors.forename: A N
authors.initials: AN
authors.lastname: Bonchuk
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000235:genetics / Q000378:metabolism*; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D004331:Drosophila melanogaster; D020652:Peptide Elongation Factor 2 / Q000235:genetics / Q000378:metabolism*; D000072417:Protein Domains; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D020652:Peptide Elongation Factor 2; D014157:Transcription Factors; C121540:dwg protein, Drosophila; C406415:grau protein, Drosophila
keywords: Drosophila; EF1α1; GST-pulldown; ZAD domain; insulators; mass spectrometry; transcription factors