title: Active and Passive Immunization Against Staphylococcus aureus Periprosthetic Osteomyelitis in Rats.
abstract: Staphylococcus aureus infection associated with orthopedic implants cannot always be controlled. We used a knee prosthesis model with implant-related osteomyelitis in rats to explore induction of an effective immune response with active and passive immunization.
  
  
  
  Fifty-two Sprague-Dawley rats were divided into active (N=28) and passive immunization groups (N=24). A bacterial inoculum of 103S. aureus MN8 was injected into the tibia and the femur marrow before insertion of a non-constrained knee prosthesis in each rat. The active-immunization group received a synthetic oligosaccharide of polysaccharide poly-N-acetylglucosamine (PNAG), 9G1cNH2and the passive-immunization group received immunization with immunoglobulin from rabbits infected with S. aureus.
  
  
  
  Active immunization against PNAG significantly reduced the consequences of osteomyelitis infection from PNAG-producing intercellular adhesion (ica+) but not ica-S. aureus. Passive immunization resulted in better clinical assessments in animals challenged with either ica+or ica-S. aureus, suggesting a lack of specificity in this antiserum.
authors.affiliation: Hand Section, Department of Orthopaedics, Herlev and Gentofte University Hospital, Hellerup, Denmark Niels.Soee.Nielsen@regionh.dk.
authors.forename: Niels H
authors.initials: NH
authors.lastname: Søe
authors.affiliation: Department of Anaesthesiology, Intensive Care and Operations, Herlev and Gentofte University Hospital, Hellerup, Denmark.
authors.forename: Nina Vendel
authors.initials: NV
authors.lastname: Jensen
authors.affiliation: Biochemical Department, Faculty of Life Science, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Asger Lundorff
authors.initials: AL
authors.lastname: Jensen
authors.affiliation: Department of Experimental Medicine, Faculty of Health Science, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Janne
authors.initials: J
authors.lastname: Koch
authors.affiliation: Biomedical Department, Panum Institute, University of Copenhagen, Copenhagen, Denmark.
authors.forename: Steen Seier
authors.initials: SS
authors.lastname: Poulsen
authors.affiliation: Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, U.S.A.
authors.forename: Gerald B
authors.initials: GB
authors.lastname: Pier
authors.affiliation: Department of Clinical Microbiology, Rigshospitalet, Copenhagen University, Copenhagen, Denmark.|The Novo Nordisk Foundation, Center for Biosustainability, Technical University of Denmark, Hørsholm, Denmark.
authors.forename: Helle Krogh
authors.initials: HK
authors.lastname: Johansen
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000032:analysis; D007114:Immunization / Q000379:methods*; D008297:Male; D010019:Osteomyelitis / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D057068:Periprosthetic Fractures / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D011817:Rabbits; D051381:Rats; D017207:Rats, Sprague-Dawley; D013203:Staphylococcal Infections / Q000276:immunology / Q000382:microbiology / Q000517:prevention & control*; D013211:Staphylococcus aureus / Q000276:immunology* / Q000472:pathogenicity
chemical_list: D015415:Biomarkers
keywords: Osteomyelitis; ica+ strain; ica− strain; immunization; implant-related infection Staphylococcus aureus; vaccine