title: Influence of resveratrol on endoplasmic reticulum stress and expression of adipokines in adipose tissues/adipocytes induced by high-calorie diet or palmitic acid.
abstract: OBJECTIVE
  This study aimed to determine whether resveratrol treatment alleviates endoplasmic reticulum stress and changes the expression of adipokines in adipose tissues and cells.
  
  
  METHODS
  8-week-old male C57BL/6 mice were fed a high-calorie diet (HCD group) or high-calorie diet supplemented with resveratrol (high-calorie diet  + resveratrol group) for 3 months. Insulin resistance, serum lipids and proinflammatory indices, the size and inflammatory cell infiltration in subcutaneous and visceral adipose tissues were analyzed. The gene expressions of endoplasmic reticulum stress, adipokines, and inflammatory cytokines were determined. The induced mature 3T3-L1 cells were pretreated with resveratrol and then palmitic acid, and the gene expressions of endoplasmic reticulum stress, adipokines, and inflammatory cytokines were determined.
  
  
  RESULTS
  Subcutaneous and visceral adipose tissues in the high-calorie diet-fed mice exhibited adipocyte hypertrophy, inflammatory activation, and endoplasmic reticulum stress. Resveratrol alleviated high-calorie diet-induced insulin resistance and endoplasmic reticulum stress, increased expression of SIRT1, and reversed expression of adipokines in varying degrees in both subcutaneous and visceral adipose tissues. The effects of resveratrol on palmitic acid-treated adipocytes were similar to those shown in the tissues.
  
  
  CONCLUSIONS
  Resveratrol treatment obviously reversed adipocyte hypertrophy and insulin resistance by attenuating endoplasmic reticulum stress and inflammation, thus increasing the expression of SIRT1 and inverting the expression of adipokines in vivo and in vitro.
authors.affiliation: Clinical Laboratory, Anhui Provincial Hospital, Hefei, Anhui, 230001, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chen
authors.affiliation: Endocrinology Department, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Anhui Medical University, Hefei, Anhui, 230032, China.
authors.forename: Guanjun
authors.initials: G
authors.lastname: Chen
authors.affiliation: Endocrinology Department, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China.
authors.forename: Nuojin
authors.initials: N
authors.lastname: Wang
authors.affiliation: The Comprehensive Laboratory, Anhui Medical University, Hefei, Anhui, 230032, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gui
authors.affiliation: Endocrinology Department, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Dai
authors.affiliation: Department of Endocrinology, The First Affiliated Hospital of Anhui University of Traditional Chinese Medicine, Hefei, Anhui, 230032, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Fang
authors.affiliation: Endocrinology Department, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui, 230022, China.
authors.forename: Qiu
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: The Comprehensive Laboratory, Anhui Medical University, Hefei, Anhui, 230032, China. wwwdluyx@sina.com.|Department of Biochemistry and Molecular Biology, Anhui Medical University, Hefei, Anhui, 230031, China. wwwdluyx@sina.com.
authors.forename: Yunxia
authors.identifier: http://orcid.org/0000-0002-8739-7789
authors.initials: Y
authors.lastname: Lu
mesh_terms: D041721:3T3-L1 Cells; D017667:Adipocytes / Q000187:drug effects* / Q000378:metabolism; D054392:Adipokines / Q000378:metabolism*; D000273:Adipose Tissue / Q000187:drug effects* / Q000378:metabolism; D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D007333:Insulin Resistance / Q000502:physiology; D008055:Lipids / Q000097:blood; D008297:Male; D051379:Mice; D019308:Palmitic Acid / Q000494:pharmacology*; D000077185:Resveratrol; D013267:Stilbenes / Q000494:pharmacology*
chemical_list: D054392:Adipokines; D008055:Lipids; D013267:Stilbenes; D019308:Palmitic Acid; D000077185:Resveratrol
keywords: 3T3L1; Adipose tissues; Endoplasmic reticulum stress; High-calorie diet; Palmitic acid; Resveratrol