title: Effect of Inulin on Proteome Changes Induced by Pathogenic Lipopolysaccharide in Human Colon.
abstract: In the present study, the protective role of inulin against lipopolysaccharide (LPS)-induced oxidative stress was evaluated on human colonic mucosa using a proteomic approach. Human colonic mucosa and submucosa were sealed between two chambers, with the luminal side facing upwards and overlaid with Krebs (control), LPS or LPS+ inulin IQ solution. The solutions on the submucosal side (undernatants) were collected following 30 min of mucosal exposure. iTRAQ based analysis was used to analyze the total soluble proteomes from human colonic mucosa and submucosa treated with different undernatants. Human colonic muscle strips were exposed to the undernatants to evaluate the response to acetylcholine. Inulin exposure was able to counteract, in human colonic mucosa, the LPS-dependent alteration of some proteins involved in the intestinal contraction (myosin light chain kinase (MLCK), myosin regulatory subunit (MYL)), to reduce the up-regulation of two proteins involved in the radical-mediated oxidative stress (the DNA-apurinic or apyrimidinic site) lyase) APEX1 and the T-complex protein 1 subunit eta (CCT7) and to entail a higher level of some detoxification enzymes (the metallothionein-2 MT2A, the glutathione-S-transferase K GSTk, and two UDP- glucuronosyltransferases UGT2B4, UGT2B17). Inulin exposure was also able to prevent the LPS-dependent intestinal muscle strips contraction impairment and the mucosa glutathione level alterations. Exposure of colonic mucosa to inulin seems to prevent LPS-induced alteration in expression of some key proteins, which promote intestinal motility and inflammation, reducing the radical-mediated oxidative stress.
authors.affiliation: Gastroenterology Unit, University Campus Bio-Medico di Roma, Rome, Italy.
authors.forename: Michele Pier Luca
authors.initials: MP
authors.lastname: Guarino
authors.affiliation: Gastroenterology Unit, University Campus Bio-Medico di Roma, Rome, Italy.
authors.forename: Annamaria
authors.identifier: http://orcid.org/0000-0001-8630-7836
authors.initials: A
authors.lastname: Altomare
authors.affiliation: Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Barera
authors.affiliation: Food Sciences and Human Nutrition Unit, University Campus Bio-Medico di Roma, Rome, Italy.
authors.forename: Vittoria
authors.initials: V
authors.lastname: Locato
authors.affiliation: Gastroenterology Unit, University Campus Bio-Medico di Roma, Rome, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Cocca
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.|Proteomics Center, University of Padova and Azienda Ospedaliera di Padova, Padova, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Franchin
authors.affiliation: Department of Biomedical Sciences, University of Padova, Padova, Italy.|Proteomics Center, University of Padova and Azienda Ospedaliera di Padova, Padova, Italy.
authors.forename: Giorgio
authors.initials: G
authors.lastname: Arrigoni
authors.affiliation: Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.
authors.forename: Candida
authors.initials: C
authors.lastname: Vannini
authors.affiliation: Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.
authors.forename: Sarah
authors.initials: S
authors.lastname: Grossi
authors.affiliation: Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.
authors.forename: Paola
authors.initials: P
authors.lastname: Campomenosi
authors.affiliation: Food Sciences and Human Nutrition Unit, University Campus Bio-Medico di Roma, Rome, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Pasqualetti
authors.affiliation: Department of Biotechnology and Life Science, University of Insubria, Varese, Italy.
authors.forename: Marcella
authors.initials: M
authors.lastname: Bracale
authors.affiliation: Surgery Unit, University Campus Bio-Medico di Roma, Roma, Italy.
authors.forename: Rossana
authors.initials: R
authors.lastname: Alloni
authors.affiliation: Food Sciences and Human Nutrition Unit, University Campus Bio-Medico di Roma, Rome, Italy.
authors.forename: Laura
authors.initials: L
authors.lastname: De Gara
authors.affiliation: Gastroenterology Unit, University Campus Bio-Medico di Roma, Rome, Italy.
authors.forename: Michele
authors.initials: M
authors.lastname: Cicala
mesh_terms: D003106:Colon / Q000187:drug effects* / Q000276:immunology / Q000378:metabolism*; D005978:Glutathione; D006801:Humans; D008070:Lipopolysaccharides / Q000276:immunology; D009119:Muscle Contraction / Q000187:drug effects; D009130:Muscle, Smooth / Q000187:drug effects / Q000378:metabolism; D018384:Oxidative Stress; D020543:Proteome / Q000187:drug effects*; D040901:Proteomics / Q000379:methods
chemical_list: D008070:Lipopolysaccharides; D020543:Proteome; D005978:Glutathione