title: Expression and localization of the AT1and AT2angiotensin II receptors and α1Aand α1Dadrenergic receptors in aorta of hypertensive and diabetic rats.
abstract: Hypertension and diabetes are multifactorial diseases that frequently coexist and exacerbate each another. During the development of diabetes, the impairment of noradrenergic and renin-angiotensin systems has been reported in the response mediated by α1-AR and AT1receptors. Although their participation in the development of cardiovascular complications is still controversial, some studies have found increased or diminished response to the vasoconstrictive effect of noradrenaline or angiotensin II in a time-dependent manner of diabetes. Thus, the aim of this work was to investigate the possible changes in the expression or localization of α1-AR (α1Aand α1D) and angiotensin II receptors (AT1and AT2) in aorta of rats after 4 weeks of the onset of diabetes. In order to be able to examine the expression of these receptors, immunofluorescence procedure was performed in tunica intima and tunica media of histological sections of aorta. Fluorescence was detected by a confocal microscopy. Our results showed that the receptors are expressed in both tunics, where adrenergic receptors have a higher density in tunica intima and tunica media of SHR compared with WKY; meanwhile, the expression of angiotensin II receptors is not modified in both groups of rats. On the other hand, the results showed that diabetes produced an increase or a decrease in the expression of receptors that is not associated to a specific type of receptor, vascular region, or strain of rat. In conclusion, diabetes and hypertension modify the expression of the receptors in tunica intima and tunica media of aorta in a different way.
authors.affiliation: a Laboratorio de Señalización Intracelular, Sección de Posgrado , Escuela Superior de Medicina del Instituto Politécnico Nacional , Ciudad de México , México.
authors.forename: Jessica Edith
authors.initials: JE
authors.lastname: Rodríguez
authors.affiliation: a Laboratorio de Señalización Intracelular, Sección de Posgrado , Escuela Superior de Medicina del Instituto Politécnico Nacional , Ciudad de México , México.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Romero-Nava
authors.affiliation: a Laboratorio de Señalización Intracelular, Sección de Posgrado , Escuela Superior de Medicina del Instituto Politécnico Nacional , Ciudad de México , México.
authors.forename: Aldo Arturo
authors.initials: AA
authors.lastname: Reséndiz-Albor
authors.affiliation: b Laboratorio de Hematopatología, Escuela Nacional de Ciencias Biológicas del Instituto Politécnico Nacional , Ciudad de México , México.
authors.forename: Erika
authors.initials: E
authors.lastname: Rosales-Cruz
authors.affiliation: c Departamento de Farmacobiología, Centro de Investigación y de Estudios Avanzados , Ciudad de México , México.
authors.forename: Enrique
authors.initials: E
authors.lastname: Hong
authors.affiliation: d Departamento de Farmacología y Toxicología, Hospital Infantil de México Federico Gómez (HIMFG) , Ciudad de México , México.
authors.forename: Fengyang
authors.initials: F
authors.lastname: Huang
authors.affiliation: a Laboratorio de Señalización Intracelular, Sección de Posgrado , Escuela Superior de Medicina del Instituto Politécnico Nacional , Ciudad de México , México.
authors.forename: Santiago
authors.initials: S
authors.lastname: Villafaña
mesh_terms: D000804:Angiotensin II / Q000378:metabolism; D000818:Animals; D001011:Aorta / Q000378:metabolism*; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism*; D006973:Hypertension / Q000378:metabolism*; D008297:Male; D051381:Rats; D011918:Rats, Inbred SHR; D011921:Rats, Inbred WKY; D044140:Receptor, Angiotensin, Type 1 / Q000378:metabolism*; D044139:Receptor, Angiotensin, Type 2 / Q000378:metabolism*; D018340:Receptors, Adrenergic, alpha-1 / Q000378:metabolism*; D012084:Renin-Angiotensin System / Q000187:drug effects; D017539:Tunica Intima / Q000378:metabolism; D017540:Tunica Media / Q000378:metabolism
chemical_list: D044140:Receptor, Angiotensin, Type 1; D044139:Receptor, Angiotensin, Type 2; D018340:Receptors, Adrenergic, alpha-1; D000804:Angiotensin II
keywords: angiotensin II receptors; hypertension; tunica intima; tunica media; type I diabetes; α1-Adrenergic receptors