title: [Bovine lactoferrin decreases the invasion of Salmonella enterica to HEp-2 cells].
abstract: OBJECTIVE
  To assess the effect of bovine lactoferrin (bLf) on the invasion of Salmonella enterica ser. Typhimurium to HEp-2 cells.
  
  
  METHODS
  HEp-2 monolayers were infected with 106 colony forming unit (CFU) of bacteria in the absence and presence of 1 and 10 mg/mL of bLf (iron-saturated) and incubated 1.5 hours at 37°C. Two treatments were evaluated: pre- infection (HEp-2 cells were incubated with bLf one hour prior to infection with Salmonella) and post-infection (bLf was added 15 minutes after the infection). Invasiveness of Salmonella was determined throgh quantification of CFU recovered from inside the HEp-2 cells (after treatment with 100 μg/mL and 10 μg/mL of gentamicin and Triton X -100).
  
  
  RESULTS
  In the pre-infection treatment, we observed a decrease of 23% of Salmonella invasion when HEp-2 cells were pre incubated with 1 mg/mL of bLf (2.8x105 vs 2.1x105, p=0.04) and 50% when them were pre-incubated with 10 mg/mL of bLf (2.8x105 vs 1.4x105, p=0.04). In post-infection treatment, no changes were observed in the invasiveness of Salmonella.
  
  
  CONCLUSIONS
  The results indicated that bLf reduces the invasiveness of Salmonella enterica ser. Typhimurium to HEp-2 cells in the pre-infection treatment.
authors.affiliation: Facultad de Farmacia y Bioquímica, Universidad Nacional Mayor de San Marcos. Lima, Perú.
authors.forename: Liz J
authors.initials: LJ
authors.lastname: Barreto Arce
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia. Lima, Perú.
authors.forename: Carmen A
authors.initials: CA
authors.lastname: Contreras García
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia. Lima, Perú.
authors.forename: David
authors.initials: D
authors.lastname: Durand Vara
authors.affiliation: Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia. Lima, Perú.
authors.forename: Theresa
authors.initials: T
authors.lastname: Ochoa Woodell
mesh_terms: D000818:Animals; D002417:Cattle; D045744:Cell Line, Tumor; D004305:Dose-Response Relationship, Drug; D006801:Humans; D007781:Lactoferrin / Q000276:immunology / Q000494:pharmacology*; D012486:Salmonella typhimurium / Q000187:drug effects* / Q000472:pathogenicity*; D014774:Virulence / Q000187:drug effects
chemical_list: D007781:Lactoferrin