title: Electrochemical manipulation of cell populations supported by biodegradable polymeric nanosheets for cell transplantation therapy.
abstract: We describe an electrochemical method of harvesting cells cultured on a biodegradable polymeric nanosheet (cell/nanosheet construct), which is stabilized on a self-assembled monolayer (SAM) of thiol molecules. A poly(lactic-co-glycolic acid) (PLGA) nanosheet was attached by hydrophobic interactions onto the surface of a SAM of l-cysteine coated onto a gold electrode. Retinal pigment epithelial cell lines (RPE-J cells) were cultured on the nanosheet to form a monolayer. An AA-size dry battery was used to apply a negative electrical potential, causing reductive desorption of the SAM from the gold surface. Within one minute of application of the voltage, the cell/nanosheet of several mm in diameter was successfully detached without the loss of cell viability in a gentle stream of the electrolyte solution. The use of a porous electrode shortened the detachment time due to the more efficient permeation of the electrolyte solution to the electrode surface. Cell transplantation following the harvesting process was demonstrated by the local delivery of RPE-J cell/nanosheet constructs into the subretinal space of rat eyes through a capillary needle. This nanosheet-based approach that allows the on-demand harvesting of cell/nanosheet constructs and their subsequent transplantation in a minimally-invasive manner could play an important role in cell transplantation therapy.
authors.affiliation: Department of Finemechanics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai 980-8579, Japan. kaji@biomems.mech.tohoku.ac.jp.
authors.forename: Jin
authors.initials: J
authors.lastname: Suzuki
authors.affiliation: Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Nagai
authors.affiliation: Department of Finemechanics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai 980-8579, Japan. kaji@biomems.mech.tohoku.ac.jp.
authors.forename: Matsuhiko
authors.initials: M
authors.lastname: Nishizawa
authors.affiliation: Division of Clinical Cell Therapy, United Centers for Advanced Research and Translational Medicine (ART), Tohoku University Graduate School of Medicine, 2-1 Seiryo, Aoba-ku, Sendai 980-8575, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Abe
authors.affiliation: Department of Finemechanics, Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki, Aoba-ku, Sendai 980-8579, Japan. kaji@biomems.mech.tohoku.ac.jp.
authors.forename: Hirokazu
authors.identifier: http://orcid.org/0000-0003-2566-4172
authors.initials: H
authors.lastname: Kaji
mesh_terms: D000818:Animals; D002470:Cell Survival; D017690:Cell Transplantation; D002478:Cells, Cultured; D055664:Electrochemical Techniques; D004566:Electrodes; D006046:Gold / Q000737:chemistry; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry / Q000378:metabolism*; D008297:Male; D049329:Nanostructures / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry / Q000378:metabolism*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D013438:Sulfhydryl Compounds / Q000737:chemistry
chemical_list: D013438:Sulfhydryl Compounds; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D006046:Gold