title: Metabolism variation and better storability of dark- versus light-coloured soybean (Glycine max L. Merr.) seeds.
abstract: The effects of storage duration on the seed germination and metabolite profiling of soybean seeds with five different coloured coats were studied. Their germination, constituents and transcript expressions of isoflavones and free fatty acids (FFAs) were compared using chromatographic metabolomic profiling and transcriptome sequencing. The seed water content was characterized using nuclear magnetic resonance (NMR) relaxometry. Results showed that dark-coloured seeds were less inactivated than light-coloured seeds. The aglycone and β-glucoside concentrations of upstream constituents increased significantly, whereas the acetylglucosides and malonylglucosides of downstream constituents decreased with an increase in the storage period. FFAs increased considerably in the soybean seeds as a result of storage. These results indicate that dark-coloured soybean seeds have better storability than light-coloured seeds, and seed water content plays a role in seed inactivation. It was concluded that there are certain metabolic regularities that are associated with different coloured seed coats of soybeans under storage conditions.
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China; Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, China. Electronic address: jiangliu@sicau.edu.cn.
authors.forename: Jiang
authors.initials: J
authors.lastname: Liu
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Wen-Ting
authors.initials: WT
authors.lastname: Qin
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Hai-Jun
authors.initials: HJ
authors.lastname: Wu
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Cai-Qiong
authors.initials: CQ
authors.lastname: Yang
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Jun-Cai
authors.initials: JC
authors.lastname: Deng
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Nasir
authors.initials: N
authors.lastname: Iqbal
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China; Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Wei-Guo
authors.initials: WG
authors.lastname: Liu
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China; Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Jun-Bo
authors.initials: JB
authors.lastname: Du
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China; Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu 611130, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Shu
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yang
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Xiao-Chun
authors.initials: XC
authors.lastname: Wang
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China.
authors.forename: Tai-Wen
authors.initials: TW
authors.lastname: Yong
authors.affiliation: Key Laboratory of Crop Ecophysiology and Farming System in Southwest, Ministry of Agriculture, Chengdu 611130, China. Electronic address: mssiyangwy@sicau.edu.cn.
authors.forename: Wen-Yu
authors.initials: WY
authors.lastname: Yang
mesh_terms: D061353:Food Storage / Q000379:methods; D018525:Germination / Q000502:physiology*; D007529:Isoflavones / Q000032:analysis / Q000378:metabolism; D010860:Pigments, Biological / Q000032:analysis* / Q000378:metabolism; D012639:Seeds / Q000737:chemistry* / Q000378:metabolism; D013025:Glycine max / Q000737:chemistry* / Q000378:metabolism; D014867:Water / Q000032:analysis
chemical_list: D007529:Isoflavones; D010860:Pigments, Biological; D014867:Water
keywords: Coat colour; Fatty acid; Isoflavone; Soybean; Storage