title: Micro RNA-19a suppresses thrombospondin-1 in CD35+B cells in the intestine of mice with food allergy.
abstract: Disruption of immune tolerance is associated in the pathogenesis of allergy. Thrombospondin-1 (TSP1) plays a role in the maintenance of immune tolerance, which is compromised in allergic disorders. Micro RNA (miR) is involved in the regulation of immune responses. This study tests a hypothesis that miR-17-92 cluster is involved in the regulation of TSP1 in the intestinal CD35+B cells. In this study, a food allergy mouse model was developed. The intestinal B cells were isolated to be analyzed for the expression of a miR-17-92 cluster and TSP1. The role of miR-19a in the suppression of TSP1 in B cells was tested in a cell culture model. We observed that the levels of TSP1 were significantly decreased; the levels of miR-19a were significantly increased in intestinal CD35+B cells of mice sensitized to ovalbumin (OVA) as compared with naïve controls. Exposure to interleukin (IL)-4 suppressed the expression of TSP1 in B cells, which was abolished by inhibition of miR-19a. miR-19a mediated the effects of IL-4 on repressing TSP1 expression in B cells. We conclude that IL-4 suppresses the expression of TSP1 in the intestinal CD35+B cells via up regulating miR-19a. The miR-19a may be a target to regulate the immune tolerant status in the body.
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Shenzhen ENT Institute, Longgang ENT HospitalShenzhen 518116, China; Brain Body Institute, McMaster UniversityHamilton, ON L8N 4A6, Canada.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Yang
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine Shenzhen 518060, China.
authors.forename: Xiao-Xi
authors.initials: XX
authors.lastname: Li
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Shenzhen ENT Institute, Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Shu-Qi
authors.initials: SQ
authors.lastname: Qiu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine Shenzhen 518060, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Zeng
authors.affiliation: Brain Body Institute, McMaster UniversityHamilton, ON L8N 4A6, Canada; Department of Gastroenterology, The Second Hospital, Zhengzhou UniversityZhengzhou, China.
authors.forename: Lin-Jing
authors.initials: LJ
authors.lastname: Li
authors.affiliation: Department of Gastroenterology, The Second Hospital, Zhengzhou University Zhengzhou, China.
authors.forename: Bai-Sui
authors.initials: BS
authors.lastname: Feng
authors.affiliation: Department of Gastroenterology, The Fifth Hospital, Zhengzhou University Zhengzhou, China.
authors.forename: Peng-Yuan
authors.initials: PY
authors.lastname: Zheng
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine Shenzhen 518060, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine Shenzhen 518060, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
keywords: B cell; Micro RNA-19a; food allergy; intestine; thrombospondin-1