title: On-Chip Magnetic Particle-Based Immunoassays Using Multilaminar Flow for Clinical Diagnostics.
abstract: Magnetic particles have become popular in recent years for immunoassays due to their high surface-to-volume ratio and the ease of their manipulation. However, such assays also require multiple reaction and washing steps that are both time-consuming and manually laborious. Here, we describe a setup and methodology for performing rapid immunoassays on magnetic particles in continuous flow via their deflection through multiple laminar flow streams of reagents and washing solutions. In particular, we focus on the use of the microfluidic platform for a C-reactive protein (CRP) sandwich immunoassay in less than 60 s.
authors.affiliation: Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK.
authors.forename: Mark D
authors.initials: MD
authors.lastname: Tarn
authors.affiliation: Department of Chemistry, The University of Hull, Cottingham Road, Hull, HU6 7RX, UK. n.pamme@hull.ac.uk.
authors.forename: Nicole
authors.initials: N
authors.lastname: Pamme
mesh_terms: D015415:Biomarkers; D004867:Equipment Design; D006801:Humans; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*
chemical_list: D015415:Biomarkers
keywords: Continuous flow; Deflection; Immunoassays; Microfluidics; Microparticles; Multilaminar flow