title: Anti-oxidative effects of curcumin on immobilization-induced oxidative stress in rat brain, liver and kidney.
abstract: Restraint stress has been indicated to induce oxidative damage in tissues. Several investigations have reported that curcumin (CUR) may have a protective effect against oxidative stress. The present study was designed to investigate the protective effects of CUR on restraint stress induced oxidative stress damage in the brain, liver and kidneys. For chronic restraint stress, rats were kept in the restrainers for 1h every day, for 21 consecutive days. The animals received systemic administrations of CUR daily for 21days. In order to evaluate the changes of the oxidative stress parameters following restraint stress, the levels of malondialdehyde (MDA), reduced glutathione (GSH), as well as antioxidant enzyme activities superoxide dismutase (SOD) glutathione peroxidase (GPx), glutathione reductase (GR) and catalase (CAT) were measured in the brain, liver and kidney of rats after the end of restraint stress. The restraint stress significantly increased MDA level, but decreased the level of GSH and activists of SOD, GPx, GR, and CAT the brain, liver and kidney of rats in comparison to the normal rats (P<0.001). Intraperitoneal administration of CUR significantly attenuated oxidative stress and lipid peroxidation, prevented apoptosis, and increased antioxidant defense mechanism activity in the tissues versus the control group (P<0.05). This study shows that CUR can prevent restraint stress-induced oxidative damage in the brain, liver and kidney of rats and propose that CUR may be useful agents against oxidative stress in the tissues.
authors.affiliation: Department of Basic Medical Sciences, Neyshabur University of Medical Sciences, Neyshabur, Iran. Electronic address: samarghandians@mums.ac.ir.
authors.forename: Saeed
authors.initials: S
authors.lastname: Samarghandian
authors.affiliation: Department of Medical Genetics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Azimi-Nezhad
authors.affiliation: Department of Immunogenetics, BuAli Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Tahereh
authors.initials: T
authors.lastname: Farkhondeh
authors.affiliation: Department of Neurosurgery, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Fariborz
authors.initials: F
authors.lastname: Samini
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D001921:Brain / Q000187:drug effects / Q000378:metabolism*; D003474:Curcumin / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D007103:Immobilization / Q000009:adverse effects; D007668:Kidney / Q000187:drug effects / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism*; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D011897:Random Allocation; D051381:Rats; D017208:Rats, Wistar; D016896:Treatment Outcome
chemical_list: D000975:Antioxidants; D003474:Curcumin
keywords: Curcumin; Oxidative stress; Restarint stress