title: Characterization of thePorphyromonas gingivalisType IX Secretion Trans-envelope PorKLMNP Core Complex.
abstract: The transport of proteins at the cell surface of Bacteroidetes depends on a secretory apparatus known as type IX secretion system (T9SS). This machine is responsible for the cell surface exposition of various proteins, such as adhesins, required for gliding motility inFlavobacterium, S-layer components inTannerella forsythia, and tooth tissue-degrading enzymes in the oral pathogenPorphyromonas gingivalisAlthough a number of subunits of the T9SS have been identified, we lack details on the architecture of this secretion apparatus. Here we provide evidence that five of the genes encoding the core complex of the T9SS are co-transcribed and that the gene products are distributed in the cell envelope. Protein-protein interaction studies then revealed that these proteins oligomerize and interact through a dense network of contacts.
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université - CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Maxence S
authors.initials: MS
authors.lastname: Vincent
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université - CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Mickaël J
authors.initials: MJ
authors.lastname: Canestrari
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques, CNRS, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France; Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Leone
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques, CNRS, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France; Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Stathopulos
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université - CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Bérengère
authors.initials: B
authors.lastname: Ize
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université - CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France.
authors.forename: Abdelrahim
authors.initials: A
authors.lastname: Zoued
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques, CNRS, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France; Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France.
authors.forename: Christian
authors.initials: C
authors.lastname: Cambillau
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques, CNRS, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France; Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Kellenberger
authors.affiliation: Architecture et Fonction des Macromolécules Biologiques, CNRS, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France; Architecture et Fonction des Macromolécules Biologiques, Aix-Marseille Université, UMR 7257, Campus de Luminy, Case 932, 13288 Marseille Cedex 09, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Roussel
authors.affiliation: Laboratoire d'Ingénierie des Systèmes Macromoléculaires, UMR 7255, Institut de Microbiologie de la Méditerranée, Aix-Marseille Université - CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France. Electronic address: cascales@imm.cnrs.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Cascales
mesh_terms: D001426:Bacterial Proteins / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D058947:Bacterial Secretion Systems / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D016866:Bacteroidaceae Infections / Q000382:microbiology; D018360:Crystallography, X-Ray; D005798:Genes, Bacterial; D006801:Humans; D016966:Porphyromonas gingivalis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D060066:Protein Interaction Maps; D021122:Protein Subunits / Q000032:analysis / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D058947:Bacterial Secretion Systems; D021122:Protein Subunits
keywords: bacterial pathogenesis; membrane protein; microbiology; protein assembly; protein complex; protein purification; protein secretion; secretion