title: QTLs for stomatal and photosynthetic traits related to salinity tolerance in barley.
abstract: Stomata regulate photosynthesis and transpiration, and these processes are critical for plant responses to abiotic stresses such as salinity. A barley double haploid population with 108 lines derived from a cross between CM72 (salt-tolerant) and Gairdner (salt-sensitive) was used to detect quantitative trait loci (QTLs) associated with stomatal and photosynthetic traits related to salinity tolerance.
  
  
  
  A total of 11 significant QTLs (LOD > 3.0) and 11 tentative QTLs (2.5 < LOD < 3.0) were identified. These QTLs are distributed on all the seven chromosomes, except 5H and explain 9.5-17.3% of the phenotypic variation. QTLs for biomass, intercellular CO2concentration, transpiration rate and stomatal conductance under control conditions co-localised together. A QTL for biomass also co-located with one for transpiration rate under salinity stress. A linkage was found between stomatal pore area and gas exchange. A QTL for salinity tolerance also co-localised with QTLs for grain yield and biomass on chromosome 3H. Based on the draft barley genome, the candidate genes for salinity tolerance at this locus are proposed.
  
  
  
  The lack of major QTLs for gas exchange and stomatal traits under control and saline conditions indicates a complex relationship between salinity and leaf gas exchange due to the fact that these complex quantitative traits are under the control of multiple genes.
authors.affiliation: School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia.|School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.
authors.forename: Xiaohui
authors.initials: X
authors.lastname: Liu
authors.affiliation: School of Land and Food and Tasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS, 7249, Australia.
authors.forename: Yun
authors.initials: Y
authors.lastname: Fan
authors.affiliation: School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia.
authors.forename: Michelle
authors.initials: M
authors.lastname: Mak
authors.affiliation: School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Babla
authors.affiliation: School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia.
authors.forename: Paul
authors.initials: P
authors.lastname: Holford
authors.affiliation: School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia.|School of Land and Food and Tasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS, 7249, Australia.
authors.forename: Feifei
authors.initials: F
authors.lastname: Wang
authors.affiliation: College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Chen
authors.affiliation: School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia.
authors.forename: Grace
authors.initials: G
authors.lastname: Scott
authors.affiliation: School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China.
authors.forename: Gang
authors.initials: G
authors.lastname: Wang
authors.affiliation: School of Land and Food and Tasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS, 7249, Australia.
authors.forename: Sergey
authors.initials: S
authors.lastname: Shabala
authors.affiliation: School of Land and Food and Tasmanian Institute of Agriculture, University of Tasmania, Hobart, TAS, 7249, Australia. meixue.zhou@utas.edu.au.
authors.forename: Meixue
authors.initials: M
authors.lastname: Zhou
authors.affiliation: School of Science and Health, Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia. Z.Chen@westernsydney.edu.au.
authors.forename: Zhong-Hua
authors.initials: ZH
authors.lastname: Chen
mesh_terms: D018533:Biomass; D002874:Chromosome Mapping; D032461:Chromosomes, Plant; D002523:Edible Grain; D056726:Genetic Association Studies; D018745:Genome, Plant; D023281:Genomics / Q000379:methods; D001467:Hordeum / Q000235:genetics* / Q000378:metabolism*; D010788:Photosynthesis / Q000235:genetics*; D054046:Plant Stomata / Q000235:genetics*; D040641:Quantitative Trait Loci; D019655:Quantitative Trait, Heritable; D054712:Salinity; D055049:Salt Tolerance / Q000235:genetics*; D013312:Stress, Physiological / Q000235:genetics
keywords: Gas exchange; Guard cell; Hordeum vulgare L; QTL mapping; Stomatal regulation