title: Dopamine-functionalized mesoporous onion-like silica as a new matrix for immobilization of lipase Candida sp. 99-125.
abstract: Dopmine functionalized mesoporous onion-like silica (DPMS) was synthesized via a biomimetic coating, and lipase Candida sp. 99-125 (LCS) was immobilized in DPMS (LCS@DPMS) by physical adsorption in this study. The DPMS was characterized by SEM, TEM, BET and FT-IR, and it was shown that the DPMS had clear multishell structures with large surface area of 419 m2/g. The activity, pH stability, thermal stability, storage stability, and reusability of the LCS@DPMS were investigated in detail. The stabilities of LCS@DPMS were improved significantly compared to the free lipase and LCS@MS (LCS immobilized in unfunctionalized mesoporous onion-like silica by physical adsorption). All the results indicated that the DPMS had high efficiency and improved stability for lipase immobilization.
authors.affiliation: School of Maritime and Civil Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Junkai
authors.initials: J
authors.lastname: Gao
authors.affiliation: School of Chemical Engineering and Technology, Hebei University of Technology, Tianjin, 300130, China.
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: School of Maritime and Civil Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Jinshu
authors.initials: J
authors.lastname: Lu
authors.affiliation: School of Maritime and Civil Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Han
authors.affiliation: School of Maritime and Civil Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Jiajia
authors.initials: J
authors.lastname: Deng
authors.affiliation: School of Maritime and Civil Engineering, Zhejiang Ocean University, Zhoushan, 316022, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Chen
mesh_terms: D000327:Adsorption; D002175:Candida / Q000201:enzymology*; D004298:Dopamine / Q000737:chemistry*; D004795:Enzyme Stability; D004800:Enzymes, Immobilized / Q000378:metabolism*; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008049:Lipase / Q000378:metabolism*; D009584:Nitrogen / Q000737:chemistry; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D017550:Spectroscopy, Fourier Transform Infrared
chemical_list: D004800:Enzymes, Immobilized; D012822:Silicon Dioxide; D008049:Lipase; D009584:Nitrogen; D004298:Dopamine