title: Long noncoding RNA HULC promotes cell proliferation by regulating PI3K/AKT signaling pathway in chronic myeloid leukemia.
abstract: Aberrant expression of long noncoding RNA (lncRNA) HULC is associated with various human cancers. However, the role of HULC in chronic myeloid leukemia (CML) is unknown. In this study, we found that HULC was remarkably overexpressed in both leukemia cell lines and primary hematopoietic cells derived from CML patients. The increase in HULC expression was positively correlated with clinical stages in CML. Moreover, the knockdown of HULC significantly inhibited CML cell proliferation and induced apoptosis by repressing c-Myc and Bcl-2. Furthermore, inhibition of HULC enhanced imatinib-induced apoptosis of CML cells. Further experiments demonstrated that HULC silencing markedly suppressed the phosphorylation of PI3K and AKT, indicating that enhancement of imatinib-induced apoptosis by HULC inhibition is related with the reduction of c-Myc expression and inhibition of PI3K/Akt pathway activity. Furthermore, HULC could modulate c-Myc and Bcl-2 by miR-200a as an endogenous sponge. Taken together, these results reveal that HULC promotes oncogenesis in CML and suggest a potential strategy for the CML treatment.
authors.affiliation: Department of Hematology, Affiliated Hospital of Guizhou Medical University, Hematopoietic Stem Cell Transplant Center of Guizhou, Blood Diseases Diagnosis and Treatment Center of Guizhou, Guizhou 550001, China. Electronic address: Yinghaolu123@163.com.
authors.forename: Yinghao
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Hematology, Affiliated Hospital of Guizhou Medical University, Hematopoietic Stem Cell Transplant Center of Guizhou, Blood Diseases Diagnosis and Treatment Center of Guizhou, Guizhou 550001, China. Electronic address: Yanlimed@126.com.
authors.forename: Yan
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Hematology, Affiliated Hospital of Guizhou Medical University, Hematopoietic Stem Cell Transplant Center of Guizhou, Blood Diseases Diagnosis and Treatment Center of Guizhou, Guizhou 550001, China. Electronic address: xiaochaixc@yeah.net.
authors.forename: Xiao
authors.initials: X
authors.lastname: Chai
authors.affiliation: Department of Hematology, Affiliated Hospital of Guizhou Medical University, Hematopoietic Stem Cell Transplant Center of Guizhou, Blood Diseases Diagnosis and Treatment Center of Guizhou, Guizhou 550001, China. Electronic address: qiankang1@outlook.com.
authors.forename: Qian
authors.initials: Q
authors.lastname: Kang
authors.affiliation: Department of Hematology, Affiliated Hospital of Guizhou Medical University, Hematopoietic Stem Cell Transplant Center of Guizhou, Blood Diseases Diagnosis and Treatment Center of Guizhou, Guizhou 550001, China. Electronic address: pengzhao-1@outlook.com.
authors.forename: Peng
authors.initials: P
authors.lastname: Zhao
authors.affiliation: Department of Hematology, Affiliated Hospital of Guizhou Medical University, Hematopoietic Stem Cell Transplant Center of Guizhou, Blood Diseases Diagnosis and Treatment Center of Guizhou, Guizhou 550001, China. Electronic address: jiexiong_vip@21CN.com.
authors.forename: Jie
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Department of Hematology, Affiliated Hospital of Guizhou Medical University, Hematopoietic Stem Cell Transplant Center of Guizhou, Blood Diseases Diagnosis and Treatment Center of Guizhou, Guizhou 550001, China. Electronic address: Jishiwangedu@outlook.com.
authors.forename: Jishi
authors.initials: J
authors.lastname: Wang
mesh_terms: D000328:Adult; D000368:Aged; D017209:Apoptosis / Q000187:drug effects / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D019254:Genes, bcl-2; D016259:Genes, myc; D006801:Humans; D000068877:Imatinib Mesylate; D015464:Leukemia, Myelogenous, Chronic, BCR-ABL Positive / Q000188:drug therapy / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics; D008875:Middle Aged; D019869:Phosphatidylinositol 3-Kinases / Q000235:genetics / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics / Q000378:metabolism*; D062085:RNA, Long Noncoding / Q000235:genetics*; D015398:Signal Transduction / Q000235:genetics
chemical_list: C574685:HULC long non-coding RNA, human; C533896:MIRN200 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; D000068877:Imatinib Mesylate; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt
keywords: C-MYC; CML; HULC; LncRNA; PI3K/AKT; miR-200a