title: The effect of glutamate on ghrelin release in mice.
abstract: Ghrelin is abundantly produced in the stomach. Here, we found that glutamate decreased ghrelin expression and release in ghrelin-producing cells, and decreased levels of food intake and plasma acyl-ghrelin in mice. Treatment with siRNA of G protein-coupled receptor, family C, group 5, member B (GPRC5B) in ghrelin-producing cell lines completely blocked the effect of glutamate-induced ghrelin suppression. In addition, glutamate inhibited ghrelin release via the extracellular signal-regulated kinase (ERK) activity pathway, and stimulated CREB2 mRNA expression in ghrelin-producing cell lines. These results suggest that glutamate inhibits ghrelin release via ERK-CREB2 pathway. These results suggest that the GPRC5B-ERK-CREB2 pathway is involved in the inhibition of ghrelin expression and secretion in ghrelin cells.
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.
authors.forename: Rakhi
authors.initials: R
authors.lastname: Chacrabati
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Gong
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.
authors.forename: Chika
authors.initials: C
authors.lastname: Ikenoya
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Kondo
authors.affiliation: Departments of Internal Medicine (Divisions of Hypothalamic Research and Endocrinology & Metabolism) and Psychiatry, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX 75390-9077, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Zigman
authors.affiliation: Area of Life-NanoBio, Division of Strategy, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.
authors.forename: Takafumi
authors.initials: T
authors.lastname: Sakai
authors.affiliation: Area of Regulatory Biology, Division of Life Science, Graduate School of Science and Engineering, Saitama University, 255 Shimo-ohkubo, Sakuraku, Saitama 338-8570, Japan.
authors.forename: Ichiro
authors.initials: I
authors.lastname: Sakata
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004305:Dose-Response Relationship, Drug; D004435:Eating / Q000187:drug effects / Q000502:physiology; D005753:Gastric Mucosa / Q000187:drug effects* / Q000378:metabolism*; D054439:Ghrelin / Q000378:metabolism*; D018698:Glutamic Acid / Q000494:pharmacology*; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D054439:Ghrelin; D018698:Glutamic Acid
keywords: CREB2; GPRC5B; extracellular signal-regulated kinase (ERK); ghrelin; glutamate