title: Brown and White Adipose Tissue Expression of IL6, UCP1 and SIRT1 are Associated with Alterations in Clinical, Metabolic and Anthropometric Parameters in Obese Humans.
abstract: Aim:The present study aimed to analyze the expression of IL6, UCP1 and SIRT1 in adipose tissue (WAT and BAT) in association to clinical, metabolic and anthropometric parameters in obese humans.Methods:WAT and BAT samples from obese patients (n=27) were collected. IL6, UCP1 and SIRT1 markers were measured by qRT-PCR. The association between IL6, UCP1 and SIRT1 mRNA expression and anthropometric and clinical parameters were evaluated, using appropriate statistical tests.Results:Our results demonstrated that high levels of IL6 are associated with altered glucose levels in the WAT (p=0.01). In contrast, high levels of IL6 in the BAT were associated with decreased % fat (p=0.01) and fat weight (p=0.02) and increased mVO2(p=0.02) and VO2(p=0.02). For UCP1, a higher expression in the BAT was observed when compared to the WAT (p=0.0001). This gene expression was associated with lower values of BMI (p=0.03), % fat (P=0.02) and fat weight (P=0.02) and increased mVO2(p=0.041) and VO2(p=0.001). In the WAT, decreased levels of SIRT1 were associated with increased fat weight (p=0.02); in the BAT, associations were found for % fat (p=0.018) and mVO2(p=0.03).Conclusion:These results reveal different characteristics in the biological actions between WAT and BAT in obese humans. Increased levels of IL6, UCP1 and SIRT1 in the BAT were associated with metabolic parameters improvements.
authors.affiliation: Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: Antônio Sérgio Barcala
authors.initials: AS
authors.lastname: Jorge
authors.affiliation: Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: Gislaine Candida Batista
authors.initials: GC
authors.lastname: Jorge
authors.affiliation: Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: Alanna Fernandes
authors.initials: AF
authors.lastname: Paraíso
authors.affiliation: Medical School. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: Raíssa M Porto
authors.initials: RM
authors.lastname: Franco
authors.affiliation: Medical School. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: Lara Jhullian Tolentino
authors.initials: LJ
authors.lastname: Vieira
authors.affiliation: Department of Pharmacodynamics, University of Florida, Gainesville, FL, USA.
authors.forename: Aline Mourão
authors.initials: AM
authors.lastname: Hilzenderger
authors.affiliation: Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: André Luiz Sena
authors.initials: AL
authors.lastname: Guimarães
authors.affiliation: Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: João Marcus Oliveira
authors.initials: JM
authors.lastname: Andrade
authors.affiliation: Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: Alfredo M Batista
authors.initials: AM
authors.lastname: De-Paula
authors.affiliation: Nucleus of Epidemiological and Molecular Research Catrumano, Health Research Laboratory, Health Science Post-graduate Programme. Universidade Estadual de Montes Claros, Montes Claros, MG, Brazil.
authors.forename: Sergio Henrique S
authors.initials: SH
authors.lastname: Santos
mesh_terms: D002001:Adipose Tissue, Brown / Q000378:metabolism / Q000473:pathology; D052436:Adipose Tissue, White / Q000378:metabolism / Q000473:pathology; D000328:Adult; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D015850:Interleukin-6 / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000378:metabolism / Q000473:pathology; D056564:Sirtuin 1 / Q000096:biosynthesis*; D000071256:Uncoupling Protein 1 / Q000096:biosynthesis*
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; C000604371:UCP1 protein, human; D000071256:Uncoupling Protein 1; C447939:SIRT1 protein, human; D056564:Sirtuin 1