title: A New Era of Genome Integration-Simply Cut and Paste!
abstract: Genome integration is a powerful tool in both basic and applied biological research. However, traditional genome integration, which is typically mediated by homologous recombination, has been constrained by low efficiencies and limited host range. In recent years, the emergence of homing endonucleases and programmable nucleases has greatly enhanced integration efficiencies and allowed alternative integration mechanisms such as nonhomologous end joining and microhomology-mediated end joining, enabling integration in hosts deficient in homologous recombination. In this review, we will highlight recent advances and breakthroughs in genome integration methods made possible by programmable nucleases, and their new applications in synthetic biology and metabolic engineering.
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research , Singapore.
authors.forename: Zihe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research , Singapore.
authors.forename: Youyun
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research , Singapore.
authors.forename: Ee Lui
authors.initials: EL
authors.lastname: Ang
authors.affiliation: Metabolic Engineering Research Laboratory, Science and Engineering Institutes, Agency for Science, Technology and Research , Singapore.|Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign , Urbana, Illinois 61801, United States.
authors.forename: Huimin
authors.identifier: 0000-0002-9069-6739
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D053903:DNA Breaks, Double-Stranded; D059766:DNA End-Joining Repair; D004720:Endonucleases / Q000235:genetics / Q000378:metabolism; D018390:Gene Targeting; D016678:Genome; D059765:Homologous Recombination; D060847:Metabolic Engineering; D016254:Mutagenesis, Insertional
chemical_list: D004720:Endonucleases
keywords: genome integration; metabolic engineering; programmable nucleases; synthetic biology