title: Analysis of protein dynamics during cytokinesis in budding yeast.
abstract: Cytokinesis is essential for development and survival of all organisms by increasing cell number and diversity. It is a highly regulated process that requires spatiotemporal coordination of hundreds of proteins functioning in the assembly, constriction, and disassembly of a contractile actomyosin ring, targeted vesicle fusion, and localized extracellular matrix remodeling. Cytokinesis has been studied in multiple systems with a wide range of technologies to learn the common principles. In this chapter, we describe the analysis of protein dynamics during cytokinesis in the budding yeast Saccharomyces cerevisiae by several live-cell imaging methods. This, in combination with the power of yeast genetics, has yielded novel insights into the mechanism of cytokinesis. Similar approaches are increasingly used to study this fundamental process in more complex systems.
authors.affiliation: University of Pennsylvania, Philadelphia, PA, United States; Kyushu University, Fukuoka, Japan.
authors.forename: S
authors.initials: S
authors.lastname: Okada
authors.affiliation: University of Pennsylvania, Philadelphia, PA, United States; University of Groningen, Groningen, The Netherlands.
authors.forename: C
authors.initials: C
authors.lastname: Wloka
authors.affiliation: University of Pennsylvania, Philadelphia, PA, United States.
authors.forename: E
authors.initials: E
authors.lastname: Bi
mesh_terms: D000205:Actomyosin / Q000737:chemistry / Q000378:metabolism; D048749:Cytokinesis / Q000235:genetics*; D005109:Extracellular Matrix / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009218:Myosins / Q000737:chemistry / Q000378:metabolism; D012441:Saccharomyces cerevisiae / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics / Q000378:metabolism; D058112:Septins / Q000096:biosynthesis / Q000378:metabolism
chemical_list: D029701:Saccharomyces cerevisiae Proteins; D000205:Actomyosin; D058112:Septins; D009218:Myosins
keywords: Cytokinesis; Myosin; Saccharomyces cerevisiae; Septin; Septum formation