title: Cloning and Characterization ofTaTGW-7AGene Associated with Grain Weight in Wheat via SLAF-seq-BSA.
abstract: Thousand-grain weight (TGW) of wheat (Triticum aestivumL.) contributes significantly to grain yield. In the present study, a candidate gene associated with TGW was identified through specific-locus amplified fragment sequencing (SLAF-seq) of DNA bulks of recombinant inbred lines (RIL) derived from the cross between Jing 411 and Hongmangchun 21. The gene was located on chromosome 7A, designated asTaTGW-7Awith a complete genome sequence and an open reading frame (ORF). A single nucleotide polymorphism (SNP) was present in the first exon between two alleles atTaTGW-7Alocus, resulting in a Val to Ala substitution, corresponding to a change from higher to lower TGW. Cleaved amplified polymorphic sequence (CAPS) (TGW7A) and InDel (TG9) markers were developed to discriminate the two allelesTaTGW-7AaandTaTGW-7Abfor higher and lower TGW, respectively. A major QTL co-segregating withTaTGW-7Aexplained 21.7-27.1% of phenotypic variance for TGW in the RIL population across five environments. The association ofTaTGW-7Awith TGW was further validated in a natural population and Chinese mini-core collections. Quantitative real-time PCR revealed higher transcript levels ofTaTGW-7Aathan those ofTaTGW-7Abduring grain development. High frequencies of the superior alleleTaTGW-7Aafor higher TGW in Chinese mini-core collections (65.0%) and 501 wheat varieties (86.0%) indicated a strong and positive selection of this allele in wheat breeding. The molecular markersTGW7AandTG9can be used for improvement of TGW in breeding programs.
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Ming-Jian
authors.initials: MJ
authors.lastname: Hu
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Hai-Ping
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Liu
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Jia-Jia
authors.initials: JJ
authors.lastname: Cao
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Sheng-Xing
authors.initials: SX
authors.lastname: Wang
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Jiang
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Zeng-Yun
authors.initials: ZY
authors.lastname: Wu
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Xiao F
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of AgricultureHefei, China; National Wheat Improvement Center/The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural SciencesBeijing, China.
authors.forename: Xian-Chun
authors.initials: XC
authors.lastname: Xia
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of AgricultureHefei, China; Department of Biology, Saint Mary's University, HalifaxNS, Canada.
authors.forename: Gen-Lou
authors.initials: GL
authors.lastname: Sun
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Chuan-Xi
authors.initials: CX
authors.lastname: Ma
authors.affiliation: College of Agronomy, Anhui Agricultural University - Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley, The Ministry of Agriculture Hefei, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Chang
keywords: IAA; SLAF-seq; common wheat; gene expression; gene-specific marker