title: Studies on the transmission and tissue distribution of Antheraea pernyi iflavirus in the Chinese oak silkmoth Antheraea pernyi.
abstract: Antheraea pernyi Iflavirus (ApIV) is a virus that is the likely causative agent of A. pernyi Vomit Disease (AVD). The virus is maintained in A. pernyi populations as an unapparent infection by as yet unknown transmission routes. The mechanisms by which an infection results in AVD are also not yet known. Both these factors were investigated in this study. A. pernyi larvae were fed or injected with ApIV, but only injection led to active replication and spread of ApIV to the head, epidermis, hemocytes, gut, fatbody, ovary and testis. Twenty percent of ApIV-injected pupae produced ApIV-infected offspring as adults, proving that ApIV can be transmitted vertically. As a confirmation of vertical transmission occurring also in nature, 53 A. pernyi couples were collected from areas where ApIV has been detected. Eleven of these couples produced ApIV-infected offspring, again pointing to a vertical transmission of ApIV.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Geng
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, PR China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Ecology, Swedish University of Agricultural Sciences (SLU), PO Box 7044, 750 07 Uppsala, Sweden.
authors.forename: Joachim R
authors.initials: JR
authors.lastname: de Miranda
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, PR China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Qian
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116023, Liaoning, PR China.
authors.forename: Lijia
authors.initials: L
authors.lastname: An
authors.affiliation: Department of Ecology, Swedish University of Agricultural Sciences (SLU), PO Box 7044, 750 07 Uppsala, Sweden. Electronic address: olle.terenius@slu.se.
authors.forename: Olle
authors.initials: O
authors.lastname: Terenius
mesh_terms: D000818:Animals; D007304:Insect Viruses / Q000235:genetics / Q000502:physiology*; D007814:Larva / Q000821:virology; D009036:Moths / Q000821:virology*; D012328:RNA Viruses / Q000235:genetics / Q000502:physiology*; D014018:Tissue Distribution
keywords: Antheraea pernyi; ApIV; Iflavirus; Tissue distribution; Transmission