title: BEAP profiles as rapid test system for status analysis and early detection of process incidents in biogas plants.
abstract: A method was developed to quantify the performance of microorganisms involved in different digestion levels in biogas plants. The test system was based on the addition of butyrate (BCON), ethanol (ECON), acetate (ACON) or propionate (PCON) to biogas sludge samples and the subsequent analysis of CH4formation in comparison to control samples. The combination of the four values was referred to as BEAP profile. Determination of BEAP profiles enabled rapid testing of a biogas plant's metabolic state within 24 h and an accurate mapping of all degradation levels in a lab-scale experimental setup. Furthermore, it was possible to distinguish between specific BEAP profiles for standard biogas plants and for biogas reactors with process incidents (beginning of NH4+-N inhibition, start of acidification, insufficient hydrolysis and potential mycotoxin effects). Finally, BEAP profiles also functioned as a warning system for the early prediction of critical NH4+-N concentrations leading to a drop of CH4formation.
authors.affiliation: Universität Bonn, Institut für Mikrobiologie und Biotechnologie, Meckenheimer Allee 168, 53115, Bonn, Germany.
authors.forename: Sarah
authors.initials: S
authors.lastname: Refai
authors.affiliation: Universität Bonn, Institut für Mikrobiologie und Biotechnologie, Meckenheimer Allee 168, 53115, Bonn, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Berger
authors.affiliation: Universität Bonn, Institut für Mikrobiologie und Biotechnologie, Meckenheimer Allee 168, 53115, Bonn, Germany.
authors.forename: Kati
authors.initials: K
authors.lastname: Wassmann
authors.affiliation: Schaumann BioEnergy GmbH, An der Mühlenau 4, 25421, Pinneberg, Germany.
authors.forename: Melanie
authors.initials: M
authors.lastname: Hecht
authors.affiliation: Bioreact GmbH, Gierlichsstraße 6, 53840, Troisdorf, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Dickhaus
authors.affiliation: Universität Bonn, Institut für Mikrobiologie und Biotechnologie, Meckenheimer Allee 168, 53115, Bonn, Germany. udeppen@uni-bonn.de.
authors.forename: Uwe
authors.initials: U
authors.lastname: Deppenmeier
mesh_terms: D000085:Acetates / Q000378:metabolism*; D001419:Bacteria / Q000378:metabolism; D056804:Biofuels / Q000382:microbiology*; D019149:Bioreactors; D002087:Butyrates / Q000378:metabolism*; D055598:Chemical Phenomena; D000431:Ethanol / Q000378:metabolism*; D007218:Industrial Microbiology; D008697:Methane / Q000378:metabolism; D011422:Propionates / Q000378:metabolism*; D012722:Sewage / Q000737:chemistry / Q000382:microbiology
chemical_list: D000085:Acetates; D056804:Biofuels; D002087:Butyrates; D011422:Propionates; D012722:Sewage; D000431:Ethanol; D008697:Methane
keywords: Biogas; Early warning system; Methane; Process failure; Renewable energy; Renewable raw material