title: Targeted mutagenesis of Drosophila RNaseZ gene by homologous recombination.
abstract: For the first time we used a homologous recombination method to obtain complete and precise deletion of Drosophila dRNaseZ gene. In the founder line of flies in which the RNaseZ sequence was replaced by attP site, the full-length sequence of the gene was reintegrated, and its functionality was shown. This approach will allow us to generate further gene mutations in different domains of dRNaseZ protein and discover a broad spectrum and uncover functions outside of tRNA processing.
authors.affiliation: Institute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Andreenkov
authors.affiliation: Institute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia.
authors.forename: E I
authors.initials: EI
authors.lastname: Volkova
authors.affiliation: Institute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia. demakov@mcb.nsc.ru.
authors.forename: S A
authors.initials: SA
authors.lastname: Demakov
authors.affiliation: Fordham University, New York, USA.
authors.forename: X
authors.initials: X
authors.lastname: Xie
authors.affiliation: Fordham University, New York, USA.
authors.forename: E B
authors.initials: EB
authors.lastname: Dubrovsky
authors.affiliation: Institute of Molecular and Cellular Biology, Siberian Branch, Russian Academy of Sciences, pr. Akademika Lavrent'eva 8, Novosibirsk, 2630090, Russia.
authors.forename: I F
authors.initials: IF
authors.lastname: Zhimulev
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D001287:Attachment Sites, Microbiological; D015152:Blotting, Northern; D015153:Blotting, Western; D002490:Central Nervous System / Q000378:metabolism; D022202:Chromosomes, Artificial, Bacterial; D004330:Drosophila; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism; D004722:Endoribonucleases / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005821:Genetic Techniques; D006066:Gonads / Q000378:metabolism; D059765:Homologous Recombination; D060227:Imaginal Discs / Q000378:metabolism; D007814:Larva; D008297:Male; D050506:Mutant Chimeric Proteins / Q000378:metabolism; D016133:Polymerase Chain Reaction; D012313:RNA / Q000378:metabolism; D000077278:RNA, Mitochondrial; D012343:RNA, Transfer / Q000378:metabolism; D017384:Sequence Deletion
chemical_list: D029721:Drosophila Proteins; D050506:Mutant Chimeric Proteins; D000077278:RNA, Mitochondrial; D012313:RNA; D012343:RNA, Transfer; D004722:Endoribonucleases; C558385:RNaseZ protein, Drosophila