title: BCL11B gene heterozygosity causes weight loss accompanied by increased energy consumption, but not defective adipogenesis, in mice.
abstract: BCL11B is a zinc finger-type transcription factor that regulates the development of the white adipose tissue (WAT), skin, central nervous system, and immune system. BCL11B is required for proper adipocyte differentiation, and BCL11B-/- embryos at E19.5 have very low amounts of the subcutaneous WAT. Here, we demonstrated that BCL11B+/- mice have lower body weight than BCL11B+/+ mice, whereas the expression of adipogenic marker genes in the WAT was comparable between BCL11B+/+ and BCL11B+/- mice. Histological analysis indicated that BCL11B+/- mice fed a high-fat diet have much smaller white adipocytes and lipid droplets in the WAT and liver, respectively. In addition, BCL11B+/- mice had increased energy consumption under both standard and high-fat diets. Thus, this study identifies BCL11B as a regulator of energy metabolism, and it is unlikely that BCL11B functions in the WAT contribute to energy metabolism in BCL11B+/- mice.
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Inoue
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Ihara
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Tsukamoto
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Yasumoto
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.|b Institute of Gerontology , The University of Tokyo , Tokyo , Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Hashidume
authors.affiliation: c Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Kenya
authors.initials: K
authors.lastname: Kamimura
authors.affiliation: d Faculty of Medicine, Department of Medical Technology, School of Health Sciences , Niigata University , Niigata , Japan.
authors.forename: Shigeki
authors.initials: S
authors.lastname: Hirano
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
authors.forename: Makoto
authors.initials: M
authors.lastname: Shimizu
authors.affiliation: e Division of Molecular Biology, Department of Molecular Genetics, Graduate School of Medical and Dental Sciences , Niigata University , Niigata , Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Kominami
authors.affiliation: a Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Tokyo , Japan.
authors.forename: Ryuichiro
authors.initials: R
authors.lastname: Sato
mesh_terms: D050156:Adipogenesis / Q000235:genetics*; D052436:Adipose Tissue, White / Q000166:cytology / Q000378:metabolism; D000818:Animals; D001835:Body Weight / Q000235:genetics*; D002454:Cell Differentiation / Q000235:genetics; D059305:Diet, High-Fat / Q000009:adverse effects; D004734:Energy Metabolism / Q000235:genetics*; D006579:Heterozygote; D051379:Mice; D008810:Mice, Inbred C57BL; D065626:Non-alcoholic Fatty Liver Disease / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009765:Obesity / Q000139:chemically induced / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D012097:Repressor Proteins / Q000172:deficiency / Q000235:genetics*; D025521:Tumor Suppressor Proteins / Q000172:deficiency / Q000235:genetics*
chemical_list: C511143:Bcl11b protein, mouse; D012097:Repressor Proteins; D025521:Tumor Suppressor Proteins
keywords: adipocytes; energy metabolism; fatty liver