title: [IGRA methods in the routine operation - QuantiFERON®-TB Gold or T-SPOT.TB?]
abstract: For indirect diagnosis of tuberculosis, two commercial IGRA (Interferon Gamma Release Assay) assays are available - primal QuantiFERON®-TB Gold test, new version QuantiFERON®-TB Gold Plus test (four tube, differentiation in activity CD4+ a CD8+) and T-SPOT®.TB test. Both methods are based on the same principle, but their workflows are different. In this article, both assays are compared on the collection of 284 patients. Inter-rate agreement measure showed 81.3% consistency and Cohens kappa index was calculated as 0.72. In case of discrepancy between IGRA and other methods (clinical aspects, X-ray diagnostic, etc.), results should be confirmed by second IGRA assay for correct interpretation.
authors.forename: J
authors.initials: J
authors.lastname: Amlerová
authors.forename: J
authors.initials: J
authors.lastname: Hrabák
mesh_terms: D000328:Adult; D005260:Female; D006801:Humans; D007118:Immunoassay / Q000379:methods*; D007371:Interferon-gamma / Q000097:blood*; D008297:Male; D011237:Predictive Value of Tests; D011933:Reagent Kits, Diagnostic; D014374:Tuberculin Test / Q000379:methods*; D014376:Tuberculosis / Q000097:blood / Q000175:diagnosis*
chemical_list: D011933:Reagent Kits, Diagnostic; D007371:Interferon-gamma
keywords: IGRA - Mycobacterium tuberculosis - Quantiferon - T-SPOT®.TB.