title: Susceptibility to Miltefosine in Brazilian Clinical Isolates ofLeishmania(Viannia)braziliensis.
abstract: Leishmania(Viannia)braziliensisis the main causative species of tegumentary leishmaniasis in Brazil. In this study, we evaluated the susceptibility of 16 clinical isolates ofL.(V.)braziliensisfrom different regions of Brazil to miltefosine in vitro. Half-maximal inhibitory concentrations of miltefosine varied from 22.9 to 144.2 μM against promastigotes and from 0.3 to 4.2 μM against intracellular amastigotes. No significant differences were found between isolates of different geographical origins. A clear correlation between the EC50against promastigotes and amastigotes within each isolate was found. These findings contribute to the evaluation of miltefosine's potential and limitations for the treatment of tegumentary leishmaniasis in Brazil.
authors.affiliation: Laboratório de Leishmanioses, Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Caroline R
authors.initials: CR
authors.lastname: Espada
authors.affiliation: Laboratório de Imunobiologia das Leishmanioses, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Brazil.
authors.forename: Fatima
authors.initials: F
authors.lastname: Ribeiro-Dias
authors.affiliation: Laboratório de Imunobiologia das Leishmanioses, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Brazil.
authors.forename: Miriam L
authors.initials: ML
authors.lastname: Dorta
authors.affiliation: Laboratório de Imunobiologia das Leishmanioses, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Goiânia, Brazil.
authors.forename: Ledice Inácia de Araújo
authors.initials: LIA
authors.lastname: Pereira
authors.affiliation: Centro de Pesquisas Gonçalo Moniz, Fiocruz-Bahia, Salvador, Brazil.|Serviço de Imunologia, Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil.
authors.forename: Edgar M de
authors.initials: EM
authors.lastname: Carvalho
authors.affiliation: Serviço de Imunologia, Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil.
authors.forename: Paulo R
authors.initials: PR
authors.lastname: Machado
authors.affiliation: Serviço de Imunologia, Faculdade de Medicina, Universidade Federal da Bahia, Salvador, Brazil.
authors.forename: Albert
authors.initials: A
authors.lastname: Schriefer
authors.affiliation: Laboratório de Leishmanioses, Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Jenicer K U
authors.initials: JKU
authors.lastname: Yokoyama-Yasunaka
authors.affiliation: Laboratório de Leishmanioses, Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.|Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, Brazil.
authors.forename: Adriano C
authors.initials: AC
authors.lastname: Coelho
authors.affiliation: Laboratório de Leishmanioses, Departamento de Parasitologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, Brazil.
authors.forename: Silvia R B
authors.initials: SRB
authors.lastname: Uliana
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D001938:Brazil; D002675:Child, Preschool; D004351:Drug Resistance; D006801:Humans; D020128:Inhibitory Concentration 50; D007892:Leishmania braziliensis / Q000187:drug effects* / Q000302:isolation & purification*; D008875:Middle Aged; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000627:therapeutic use; D055815:Young Adult
chemical_list: D010767:Phosphorylcholine; C039128:miltefosine