title: Anti-diabetic activities of cis- and trans-2,3,5,4'-tetrahydroxystilbene 2-O-β-glucopyranoside from Polygonum multiflorum.
abstract: Functional foods can be used alone or in combination with existing therapies in preventing and treating type 2 diabetes (T2D). Trans-2,3,5,4'-tetrahydroxystilbene 2-O-β-glucopyranoside (trans-THSG), a dominant bioactive compound from Polygonum multiflorum (PM)-a popular medicinal food in Asia, has attracted increasing research interests due to its strong antioxidant activity. The content of naturally occurring cis-THSG (cis-2,3,5,4'-tetrahydroxystilbene 2-O-β-glucopyranoside) was very low in PM root, but was prepared in this study by mimicking the traditional process of PM. The anti-diabetic effects of trans- and cis-THSG were evaluated in T2D to search for more efficacious food ingredient(s).
  
  
  
  Trans-THSG was chromatographically purified from PM roots and cis-THSG was prepared with our innovative process via exposure of trans-THSG to UV-light. The anti-diabetic effects of both THSGs were tested with HFD-induced male CF-1 diabetic mice. Cis-THSG was found more effective than trans-THSG in hypoglycemic effect and in ameliorating glucose intolerance and insulin resistance. In HepG2 cells, cis-THSG also demonstrated more potent activity than trans-THSG in suppressing transcription of phosphoenopyruvate carboxykinase (PEPCK).
  
  
  
  Cis-THSG can be an enriched bioactive ingredient in PM roots from post-processing and is significantly more effective against hyperglycemia than trans-THSG. One of the effective pathways was through inhibition of PEPCK.
authors.affiliation: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization; College of Life Science, Huanggang Normal University, Huanggang, China.|Department of Food Science, Rutgers-The State University of New Jersey, New Brunswick, NJ, USA.
authors.forename: Wenping
authors.initials: W
authors.lastname: Tang
authors.affiliation: Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resources Comprehensive Utilization; College of Life Science, Huanggang Normal University, Huanggang, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Chemical Biology, Rutgers-The State University of New Jersey, Piscataway, NJ, USA.
authors.forename: Yue
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Jia-Ching
authors.initials: JC
authors.lastname: Wu
authors.affiliation: Institute of Food Science and Technology, National Taiwan University, Taipei, Taiwan.
authors.forename: Min-Hsiung
authors.initials: MH
authors.lastname: Pan
authors.affiliation: Department of Chemical Biology, Rutgers-The State University of New Jersey, Piscataway, NJ, USA.
authors.forename: Mou-Tuan
authors.initials: MT
authors.lastname: Huang
authors.affiliation: Department of Food Science, Rutgers-The State University of New Jersey, New Brunswick, NJ, USA.
authors.forename: Chi-Tang
authors.initials: CT
authors.lastname: Ho
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy; D003924:Diabetes Mellitus, Type 2 / Q000188:drug therapy / Q000209:etiology; D059305:Diet, High-Fat / Q000009:adverse effects; D004791:Enzyme Inhibitors / Q000737:chemistry / Q000494:pharmacology; D000068676:Fallopia multiflora / Q000737:chemistry*; D005960:Glucosides / Q000737:chemistry* / Q000494:pharmacology*; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000737:chemistry / Q000494:pharmacology*; D007333:Insulin Resistance; D008297:Male; D019764:Phosphoenolpyruvate Carboxykinase (ATP) / Q000037:antagonists & inhibitors / Q000235:genetics; D013267:Stilbenes / Q000737:chemistry* / Q000494:pharmacology*
chemical_list: C000590846:2,3,5,4'-tetrahydroxystilbene-2-O-beta-D-glucopyranoside; D004791:Enzyme Inhibitors; D005960:Glucosides; D007004:Hypoglycemic Agents; D013267:Stilbenes; C000597251:PCK2 protein, human; D019764:Phosphoenolpyruvate Carboxykinase (ATP)
keywords: 2,3,5,4′-tetrahydroxystilbene glycoside; Hypoglycemic activity; PEPCK; Polygonum multiflorum; Type 2 diabetes