title: Global quantitative proteomic analysis profiles host protein expression in response to Sendai virus infection.
abstract: Sendai virus (SeV) is an enveloped nonsegmented negative-strand RNA virus that belongs to the genus Respirovirus of the Paramyxoviridae family. As a model pathogen, SeV has been extensively studied to define the basic biochemical and molecular biologic properties of the paramyxoviruses. In addition, SeV-infected host cells were widely employed to uncover the mechanism of innate immune response. To identify proteins involved in the SeV infection process or the SeV-induced innate immune response process, system-wide evaluations of SeV-host interactions have been performed. cDNA microarray, siRNA screening and phosphoproteomic analysis suggested that multiple signaling pathways are involved in SeV infection process. Here, to study SeV-host interaction, a global quantitative proteomic analysis was performed on SeV-infected HEK 293T cells. A total of 4699 host proteins were quantified, with 742 proteins being differentially regulated. Bioinformatics analysis indicated that regulated proteins were mainly involved in "interferon type I (IFN-I) signaling pathway" and "defense response to virus," suggesting that these processes play roles in SeV infection. Further RNAi-based functional studies indicated that the regulated proteins, tripartite motif (TRIM24) and TRIM27, affect SeV-induced IFN-I production. Our data provided a comprehensive view of host cell response to SeV and identified host proteins involved in the SeV infection process or the SeV-induced innate immune response process.
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Sheng-Lin
authors.initials: SL
authors.lastname: Zhu
authors.affiliation: The Laboratory of Biological Mass Spectrometry, Wuhan Institute of Biotechnology, Wuhan, P. R. China.|Medical Research Institute, Wuhan University, Wuhan, P. R. China.
authors.forename: Xi
authors.initials: X
authors.lastname: Chen
authors.affiliation: School of Chemistry and Life Sciences, Hubei University of Education, Wuhan, P. R. China.
authors.forename: Liang-Jie
authors.initials: LJ
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Wan
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Qi-Lin
authors.initials: QL
authors.lastname: Xin
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Gengfu
authors.initials: G
authors.lastname: Xiao
authors.affiliation: State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, P. R. China.
authors.forename: Lei-Ke
authors.initials: LK
authors.lastname: Zhang
mesh_terms: D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism / Q000821:virology; D057809:HEK293 Cells / Q000821:virology; D054884:Host-Pathogen Interactions / Q000502:physiology*; D006801:Humans; D007370:Interferon Type I / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000032:analysis / Q000378:metabolism; D016133:Polymerase Chain Reaction / Q000379:methods; D020543:Proteome / Q000032:analysis* / Q000235:genetics / Q000378:metabolism; D040901:Proteomics / Q000379:methods; D015203:Reproducibility of Results; D010253:Respirovirus Infections / Q000378:metabolism* / Q000821:virology; D029082:Sendai virus / Q000472:pathogenicity*; D014157:Transcription Factors / Q000378:metabolism; D000070584:Tripartite Motif Proteins / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000378:metabolism; D014779:Virus Replication
chemical_list: D007370:Interferon Type I; D009687:Nuclear Proteins; D020543:Proteome; D014157:Transcription Factors; D000070584:Tripartite Motif Proteins; C084523:TRIM25 protein, human; D044767:Ubiquitin-Protein Ligases
keywords: Innate immune response; Quantitative proteomics; Sendai virus; Virus-host interaction