title: Tissue Culture for Xylem Differentiation with Arabidopsis Leaves.
abstract: Culture systems combined with molecular biological approaches have identified various key factors regulating vascular differentiation, which makes a great contribution to development of vascular biology. Recently, we established a novel culture system for xylem cell differentiation in the model plant Arabidopsis thaliana (Arabidopsis), in which ectopic xylem cells can be induced throughout leaf disks within 3-4 days. Here we describe detailed procedures of the culture system from sample preparation to vascular cell induction culture.
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Masato
authors.initials: M
authors.lastname: Saito
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.
authors.forename: Alif Meem
authors.initials: AM
authors.lastname: Nurani
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. p@bs.s.u-tokyo.ac.jp.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Kondo
authors.affiliation: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. fukuda@bs.s.u-tokyo.ac.jp.
authors.forename: Hiroo
authors.initials: H
authors.lastname: Fukuda
mesh_terms: D017360:Arabidopsis / Q000166:cytology* / Q000254:growth & development*; D002454:Cell Differentiation; D054338:Cell Transdifferentiation; D058503:Mesophyll Cells / Q000166:cytology; D018515:Plant Leaves / Q000166:cytology* / Q000254:growth & development*; D046509:Tissue Culture Techniques; D052584:Xylem / Q000166:cytology* / Q000254:growth & development*
keywords: Arabidopsis; Culture system; Mesophyll; Procambium; Reprogramming; Transdifferentiation; Xylem