title: First in vivo MRI study on theranostic dendrimersomes.
abstract: Amphiphilic Janus-dendrimers are able to self-assemble into nanosized vesicles named dendrimersomes. We recently synthesized the 3,5-C12-EG-(OH)4dendrimer that generates dendrimersomes with very promising safety and stability profiles, that can be loaded with different contrast agents for in vivo imaging. In this contribution, nanovesicles were loaded with both the Magnetic Resonance Imaging (MRI) reporter GdDOTAGA(C18)2and the glucocorticoid drug Prednisolone Phosphate (PLP), in order to test their effective potential as theranostic nanocarriers on murine melanoma tumour models. The incorporation of GdDOTAGA(C18)2into the membrane resulted in dendrimersomes with a high longitudinal relaxivity (r1=39.1mM-1s-1, at 310K and 40MHz) so that, after intravenous administration, T1-weighted MRI showed a consistent contrast enhancement in the tumour area. Furthermore, the nanovesicles encapsulated PLP with good efficiency and displayed anti-tumour activity both in vitro and in vivo, thus enabling their practical use for biomedical theranostic applications.
authors.affiliation: Centro di Imaging Molecolare e Preclinico, Dipartimento di Biotecnologie Molecolari e Scienze della Salute, Università di Torino, Via Nizza 52, 10126 Torino, Italia. Electronic address: miriam.filippi@unito.it.
authors.forename: Miriam
authors.initials: M
authors.lastname: Filippi
authors.affiliation: Centro di Imaging Molecolare e Preclinico, Dipartimento di Biotecnologie Molecolari e Scienze della Salute, Università di Torino, Via Nizza 52, 10126 Torino, Italia. Electronic address: valeria.catanzaro@unito.it.
authors.forename: Valeria
authors.initials: V
authors.lastname: Catanzaro
authors.affiliation: Centro di Imaging Molecolare e Preclinico, Dipartimento di Biotecnologie Molecolari e Scienze della Salute, Università di Torino, Via Nizza 52, 10126 Torino, Italia. Electronic address: deyssyj@gmail.com.
authors.forename: Deyssy
authors.initials: D
authors.lastname: Patrucco
authors.affiliation: Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale "Amedeo Avogadro", Viale T. Michel 11, 15121 Alessandria, Italia. Electronic address: mauro.botta@uniupo.it.
authors.forename: Mauro
authors.initials: M
authors.lastname: Botta
authors.affiliation: Dipartimento di Scienze e Innovazione Tecnologica, Università del Piemonte Orientale "Amedeo Avogadro", Viale T. Michel 11, 15121 Alessandria, Italia. Electronic address: lorenzo.tei@uniupo.it.
authors.forename: Lorenzo
authors.initials: L
authors.lastname: Tei
authors.affiliation: Centro di Imaging Molecolare e Preclinico, Dipartimento di Biotecnologie Molecolari e Scienze della Salute, Università di Torino, Via Nizza 52, 10126 Torino, Italia. Electronic address: enzo.terreno@unito.it.
authors.forename: Enzo
authors.initials: E
authors.lastname: Terreno
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000008:administration & dosage* / Q000493:pharmacokinetics; D050091:Dendrimers / Q000737:chemistry*; D005260:Female; D005938:Glucocorticoids / Q000008:administration & dosage* / Q000493:pharmacokinetics; D006571:Heterocyclic Compounds / Q000008:administration & dosage* / Q000493:pharmacokinetics; D061307:Human Umbilical Vein Endothelial Cells; D006801:Humans; D008279:Magnetic Resonance Imaging / Q000379:methods*; D008545:Melanoma / Q000000981:diagnostic imaging* / Q000188:drug therapy; D051379:Mice; D008810:Mice, Inbred C57BL; D053769:Nanocapsules / Q000737:chemistry*; D009942:Organometallic Compounds / Q000008:administration & dosage* / Q000493:pharmacokinetics; D011239:Prednisolone / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000493:pharmacokinetics; D000068936:Theranostic Nanomedicine
chemical_list: D003287:Contrast Media; D050091:Dendrimers; D005938:Glucocorticoids; D006571:Heterocyclic Compounds; D053769:Nanocapsules; D009942:Organometallic Compounds; C009022:prednisolone phosphate; C050823:gadolinium 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraacetate; D011239:Prednisolone
keywords: Dendrimersomes; Magnetic Resonance Imaging; Melanoma; Prednisolone Phosphate; Theranostic