title: Highly versatile SPION encapsulated PLGA nanoparticles as photothermal ablators of cancer cells and as multimodal imaging agents.
abstract: We have designed versatile polymeric nanoparticles with cancer cell specific targeting capabilities via aptamer conjugation after the successful encapsulation of curcumin and superparamagnetic iron oxide nanoparticles (SPIONs) inside a PLGA nanocapsule. These targeted nanocomposites were selectively taken up by tumor cells, under in vitro conditions, demonstrating the effectiveness of the aptamer targeting mechanism. Moreover, the nanocomposite potentially functioned as efficient multiprobes for optical, magnetic resonance imaging (MRI) and photoacoustic imaging contrast agents in the field of cancer diagnostics. The hyperthermic ability of these nanocomposites was mediated by SPIONs upon NIR-laser irradiation. In vitro cytotoxicity was shown by curcumin-loaded nanoparticles as well as the photothermal ablation of cancer cells mediated by the drug-encapsulated nanocomposite demonstrated the potential therapeutic effect of the nanocomposite. In short, we portray the aptamer-conjugated nanocomposite as a multimodal material capable of serving as a contrast agent for MR, photoacoustic and optical imaging. Furthermore, the nanocomposite functions as a targetable drug nanocarrier and a NIR-laser inducible hyperthermic material that is capable of ablating PANC-1 and MIA PaCa-2 cancer cell lines.
authors.affiliation: Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Japan. sakthi@toyo.jp.
authors.forename: Balasubramanian
authors.initials: B
authors.lastname: Sivakumar
authors.affiliation: Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Japan. sakthi@toyo.jp.
authors.forename: Ravindran Girija
authors.initials: RG
authors.lastname: Aswathy
authors.affiliation: Department of Materials Science and NanoEngineering, Rice University, 6100 Main St., Houston, TX 77005, USA.
authors.forename: Rebeca
authors.initials: R
authors.lastname: Romero-Aburto
authors.affiliation: Department of Imaging Physics, MD Anderson Cancer Center, 1881 East Rd., Houston, TX 77054, USA.
authors.forename: Trevor
authors.initials: T
authors.lastname: Mitcham
authors.affiliation: Small Animal Imaging Facility, The University of Texas, MD Anderson Cancer Center, Houston, TX 77030, USA.
authors.forename: Keith A
authors.identifier: http://orcid.org/0000-0002-4330-6881
authors.initials: KA
authors.lastname: Mitchel
authors.affiliation: Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Japan. sakthi@toyo.jp.
authors.forename: Yutaka
authors.initials: Y
authors.lastname: Nagaoka
authors.affiliation: Department of Imaging Physics, MD Anderson Cancer Center, 1881 East Rd., Houston, TX 77054, USA.
authors.forename: Richard R
authors.initials: RR
authors.lastname: Bouchard
authors.affiliation: Department of Materials Science and NanoEngineering, Rice University, 6100 Main St., Houston, TX 77005, USA.
authors.forename: Pulickel M
authors.initials: PM
authors.lastname: Ajayan
authors.affiliation: Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Japan. sakthi@toyo.jp.
authors.forename: Toru
authors.initials: T
authors.lastname: Maekawa
authors.affiliation: Bio Nano Electronics Research Center, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Japan. sakthi@toyo.jp.
authors.forename: Dasappan Nair
authors.identifier: http://orcid.org/0000-0003-4819-2032
authors.initials: DN
authors.lastname: Sakthikumar
mesh_terms: D052157:Aptamers, Nucleotide / Q000737:chemistry*; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry*; D008279:Magnetic Resonance Imaging / Q000379:methods; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D064847:Multimodal Imaging / Q000379:methods; D053761:Nanocomposites / Q000737:chemistry*; D009369:Neoplasms / Q000000981:diagnostic imaging*; D061848:Optical Imaging / Q000379:methods; D061088:Photoacoustic Techniques / Q000379:methods; D011100:Polyglycolic Acid / Q000737:chemistry*; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer
chemical_list: D052157:Aptamers, Nucleotide; D003287:Contrast Media; D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid