title: Stromal Cell Subsets Directing Neonatal Spleen Regeneration.
abstract: Development of lymphoid tissue is determined by interactions between stromal lymphoid tissue organiser (LTo) and hematopoietic lymphoid tissue inducer (LTi) cells. A failure for LTo to receive appropriate activating signals during embryogenesis through lymphotoxin engagement leads to a complete cessation of lymph node (LN) and Peyer's patch development, identifying LTo as a key stromal population for lymphoid tissue organogenesis. However, little is known about the equivalent stromal cells that induce spleen development. Here, by dissociating neonatal murine spleen stromal tissue for re-aggregation and transplant into adult mouse recipients, we have identified a MAdCAM-1+CD31+CD201+spleen stromal organizer cell-type critical for new tissue formation. This finding provides an insight into the regulation of post-natal spleen tissue organogenesis, and could be exploited in the development of spleen regenerative therapies.
authors.affiliation: AK Project, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.|Division of Biomedical Science, Research School of Biology, The Australian National University, Canberra 0200, Australia.
authors.forename: Jonathan K H
authors.initials: JK
authors.lastname: Tan
authors.affiliation: AK Project, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Watanabe
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D015703:Antigens, CD / Q000378:metabolism; D015415:Biomarkers / Q000378:metabolism; D002449:Cell Aggregation; D005786:Gene Expression Regulation; D008221:Lymphoid Tissue / Q000166:cytology; D008280:Magnetics; D008648:Mesoderm / Q000166:cytology; D051379:Mice; D008807:Mice, Inbred BALB C; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012038:Regeneration / Q000502:physiology*; D013154:Spleen / Q000166:cytology* / Q000502:physiology*; D017154:Stromal Cells / Q000166:cytology
chemical_list: D015703:Antigens, CD; D015415:Biomarkers; D012333:RNA, Messenger