title: The Expression of HMGB1 in Bone Marrow MSCs Is Upregulated by Hypoxia with Regulatory Effects on the Apoptosis and Adhesion.
abstract: Background and Aims. Hypoxia regulates the survival of mesenchymal stem cells (MSCs) but the mechanism is unclear. In hypoxia, the level of high mobility group box 1 (HMGB1) was increased in many cells which may be involved in the regulation of cell biology. The aim is to determine whether hypoxia affects the expression of HMGB1 in bone marrow MSCs (BM-MSCs) and to investigate the role of HMGB1 in the apoptosis and adhesion.Methods. BM-MSCs were exposed to hypoxia (1% O2) and normoxia (20% O2) and the expression of HMGB1 was measured by RT-PCR and western blotting. The apoptosis and adhesion of BM-MSCs were evaluated after interfered by different concentrations of HMGB1.Results. Expression of HMGB1 in BM-MSCs showed a significant upregulation in hypoxia when compared to those in normoxia. The adhesion of BM-MSCs was increased by HMGB1 in a concentration-dependent manner; the apoptosis effect of HMGB1 depended on its concentrations: HMGB1 at low concentration (50 ng/mL) promoted the apoptosis of BM-MSCs while HMGB1 at high concentration (≥100 ng/mL) reduced this apoptosis.Conclusions. Hypoxia enhanced the expression of HMGB1 in BM-MSCs with influences on apoptosis and adhesion and this could have a significant effect on the regenerative potential of MSC-based strategies.
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Mei-Yun
authors.initials: MY
authors.lastname: Tan
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Cai-Dong
authors.initials: CD
authors.lastname: Zhang
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Xia
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Jiang
authors.initials: J
authors.lastname: Guo
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Fan
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Tian-Hao
authors.initials: TH
authors.lastname: Wu
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Sen
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Bone and Joint Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Shao-Feng
authors.initials: SF
authors.lastname: Liu
authors.affiliation: Laboratory of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Li
authors.initials: L
authors.lastname: Deng
authors.affiliation: Department of Burns and Plastic Surgery, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
authors.forename: Xing
authors.identifier: 0000-0002-4682-3083
authors.initials: X
authors.lastname: Guo
authors.affiliation: Shenzhen Engineering Laboratory of Orthopaedic Regenerative Technologies, Orthopaedic Research Center, Peking University Shenzhen Hospital, Shenzhen, China; Shenzhen Key Laboratory of Spine Surgery, Department of Spine Surgery, Peking University Shenzhen Hospital, Shenzhen, China; Department of Orthopaedics and Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam 999077, Hong Kong.
authors.forename: Yong-Can
authors.identifier: 0000-0001-8548-8233
authors.initials: YC
authors.lastname: Huang
mesh_terms: D000818:Animals; D017209:Apoptosis; D001854:Bone Marrow Cells / Q000166:cytology*; D002448:Cell Adhesion; D015687:Cell Hypoxia; D024243:HMGB1 Protein / Q000378:metabolism*; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism*; D017207:Rats, Sprague-Dawley; D060888:Real-Time Polymerase Chain Reaction; D015854:Up-Regulation
chemical_list: D024243:HMGB1 Protein