title: Supplementing rooster sperm with Cholesterol-Loaded-Cyclodextrin improves fertility after cryopreservation.
abstract: Little is known about the effects of Cholesterol-Loaded Cyclodextrin (CLC) on post-thaw semen quality in chicken. The aim of the present study is to investigate the efficacy of CLC levels (0, 1, 2 and 3 mg/mL Schramm diluent) on post-thawed semen quality and fertility in two breeds of chicken Pradu Hang Dum (native chicken) and Rhode Island Red. Semen samples of each breed were pooled, divided into 4 aliquots and diluted with Schramm diluents, cooled to 5 °C when DMF was added (6% of final volume). Semen straws were subjected to cryopreservation using the liquid nitrogen vapor method. Post-thawed sperm motility, viability, acrosome integrity, mitochondrial function, and the Malondialdehyde (MDA) level were determined. The fertility of frozen semen was tested by inseminating laying hens. Post-thaw motility between Pradu Hang Dum and Rhode Island Red was no different; but Rhode Island Red had a higher semen viability and live cell intact acrosomes than Pradu Hang Dum (P < 0.05). The percentage of high functioning mitochondria in the Pradu Hang Dum was higher than the Rhode Island Red. CLC at 2 and 3 mg/mL supplementation was associated with improved viability of frozen semen; that is, acrosome integrity and mitochondrial function (P < 0.01), albeit having no effect on MDA levels. The sperm with 1 mg/mL CLC yielded a significantly better fertility (P < 0.01). CLC (1 mg/mL) improved the quality of frozen rooster semen. There was no interaction among breeds and CLC on post-thaw semen quality and fertility.
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Napapach
authors.initials: N
authors.lastname: Chuaychu-Noo
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Pachara
authors.initials: P
authors.lastname: Thananurak
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Vibantita
authors.initials: V
authors.lastname: Chankitisakul
authors.affiliation: Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand; Research and Development Network Center for Animal Breeding (Native Chicken), Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand. Electronic address: vthevi@kku.ac.th.
authors.forename: Thevin
authors.initials: T
authors.lastname: Vongpralub
mesh_terms: D000177:Acrosome / Q000502:physiology; D000818:Animals; D002645:Chickens; D002784:Cholesterol / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D003505:Cyclodextrins / Q000494:pharmacology*; D005298:Fertility / Q000502:physiology; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D008928:Mitochondria / Q000187:drug effects; D012661:Semen / Q000502:physiology; D055101:Semen Analysis; D012662:Semen Preservation / Q000379:methods*; D013081:Sperm Motility / Q000187:drug effects*; D013094:Spermatozoa / Q000187:drug effects
chemical_list: D003451:Cryoprotective Agents; D003505:Cyclodextrins; D008315:Malondialdehyde; D002784:Cholesterol
keywords: Breeds; Cholesterol Load Cyclodextrin (CLC); Fertility; Frozen semen; Rooster