title: Dual-functional lipid-like nanoparticles for delivery of mRNA and MRI contrast agents.
abstract: Multi-functional nanomaterials possess unique properties, facilitating both therapeutic and diagnostic applications among others. Herein, we developed dual-functional lipid-like nanoparticles for simultaneous delivery of mRNA and magnetic resonance imaging (MRI) contrast agents in order to express functional proteins and provide real-time visualization. TT3-Gd18 LLNs were identified as a lead formulation, which was able to encapsulate 91% of mRNA and 74% of Gd. This formulation showed a comparable or a slightly higher delivery efficiency of mRNA compared to the initial TT3 LLNs. Moreover, a strong MRI signal was observed in the cell pellets treated with TT3-Gd18 LLNs. More importantly, TT3-Gd18 LLNs demonstrated an efficient delivery of mRNA and Gd contrast agents in vivo.
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA. dong.525@osu.edu.
authors.forename: X
authors.initials: X
authors.lastname: Luo
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA. dong.525@osu.edu.
authors.forename: B
authors.initials: B
authors.lastname: Li
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA. dong.525@osu.edu.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA. dong.525@osu.edu.
authors.forename: W
authors.initials: W
authors.lastname: Zhao
authors.affiliation: Small Animal Imaging Center, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: A
authors.initials: A
authors.lastname: Bratasz
authors.affiliation: Center for Electron Microscopy and Analysis, Department of Materials Science and Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: B
authors.initials: B
authors.lastname: Deng
authors.affiliation: Center for Electron Microscopy and Analysis, Department of Materials Science and Engineering, The Ohio State University, Columbus, Ohio 43210, USA.
authors.forename: D W
authors.initials: DW
authors.lastname: McComb
authors.affiliation: Division of Pharmaceutics and Pharmaceutical Chemistry, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, USA. dong.525@osu.edu.
authors.forename: Y
authors.identifier: http://orcid.org/0000-0001-5786-0659
authors.initials: Y
authors.lastname: Dong
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D003287:Contrast Media / Q000008:administration & dosage*; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D008279:Magnetic Resonance Imaging; D051379:Mice; D008810:Mice, Inbred C57BL; D053758:Nanoparticles; D012333:RNA, Messenger / Q000008:administration & dosage*
chemical_list: D003287:Contrast Media; D008055:Lipids; D012333:RNA, Messenger