title: Measurement of Thermal Conductivities of Two Cryoprotective Agent Solutions for Vitreous Cryopreservation of Organs at the Temperature Range of 77 K-300 K Using a Thermal Sensor Made of Microscale Enamel Copper Wire.
abstract: Biobanking of organs by cryopreservation is an enabling technology for organ transplantation. Compared with the conventional slow freezing method, vitreous cryopreservation has been regarded to be a more promising approach for long-term storage of organs. The major challenges to vitrification are devitrification and recrystallization during the warming process, and high concentrations of cryoprotective agents (CPAs) induced metabolic and osmotic injuries. For a theoretical model based optimization of vitrification, thermal properties of CPA solutions are indispensable. In this study, the thermal conductivities of M22 and vitrification solution containing ethylene glycol and dimethyl sulfoxide (two commonly used vitrification solutions) were measured using a self-made microscaled hot probe with enameled copper wire at the temperature range of 77 K-300 K. The data obtained by this study will further enrich knowledge of the thermal properties for CPA solutions at low temperatures, as is of primary importance for optimization of vitrification.
authors.affiliation: 1 Department of Electronic Science & Technology, University of Science and Technology of China , Hefei, China .
authors.forename: Yufang
authors.initials: Y
authors.lastname: Li
authors.affiliation: 1 Department of Electronic Science & Technology, University of Science and Technology of China , Hefei, China .|2 Anhui Provincial Engineering Technology Research Center for Biopreservation and Artificial Organs , Hefei, China .
authors.forename: Gang
authors.initials: G
authors.lastname: Zhao
authors.affiliation: 1 Department of Electronic Science & Technology, University of Science and Technology of China , Hefei, China .
authors.forename: S M Chapal
authors.initials: SMC
authors.lastname: Hossain
authors.affiliation: 1 Department of Electronic Science & Technology, University of Science and Technology of China , Hefei, China .
authors.forename: Fazil
authors.initials: F
authors.lastname: Panhwar
authors.affiliation: 3 Yinfeng Cryomedicine Technology Co., Ltd. , Jinan, China .
authors.forename: Wenyu
authors.initials: W
authors.lastname: Sun
authors.affiliation: 3 Yinfeng Cryomedicine Technology Co., Ltd. , Jinan, China .
authors.forename: Fei
authors.initials: F
authors.lastname: Kong
authors.affiliation: 3 Yinfeng Cryomedicine Technology Co., Ltd. , Jinan, China .
authors.forename: Chuanbao
authors.initials: C
authors.lastname: Zang
authors.affiliation: 1 Department of Electronic Science & Technology, University of Science and Technology of China , Hefei, China .
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Jiang
mesh_terms: D018070:Biological Specimen Banks; D003080:Cold Temperature; D003300:Copper / Q000737:chemistry*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000737:chemistry*; D008962:Models, Theoretical; D009926:Organ Preservation / Q000379:methods* / Q000592:standards; D013814:Thermal Conductivity; D058989:Vitrification
chemical_list: D003451:Cryoprotective Agents; D003300:Copper
keywords: hot probe; the Monte Carlo inversion method; the least square method; thermal conductivity; vitrification