title: Optimized microwave-assistant extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. and evaluation of its anti-allergic activity.
abstract: Microwave extraction combined ultrasonic pretreatment of flavonoids from Periploca forrestii Schltr. was investigated in this study, extraction process was first performed in an ultrasonic cleaner, then treated by microwave irradiation. The optimum ultrasonic time of 25 min was selected by single-factor experiments. A response surface methodology has been used to obtain a mathematical model that describes the process and analyzes the significant parameters ethanol concentration 59.92%, liquid to raw materials ratio 21.24 mL/g, microwave radiation time 209.53 s, and microwave power 274.14 w. In these optimum conditions, the yield of flavonoids from P. forrestii (TFPF) could be up to 9.11 ± 0.08%, which was increased by 14.30 and 19.86% compared microwave extraction and ultrasonic extraction, respectively. In vitro suppress hyaluronidase experimentation showed that TFPF purified using polyamide exhibited good anti-hyaluronidase ability with IC50value of 1.033 mg/mL, possessing certain anti-antiallergic and potential application prospect in pharmaceutical production of treating inflammation and other related fields.
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, P. R. China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liang
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, P. R. China.
authors.forename: Huaguo
authors.initials: H
authors.lastname: Chen
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, P. R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Zhou
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, P. R. China.
authors.forename: Qingfang
authors.initials: Q
authors.lastname: Deng
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, P. R. China.
authors.forename: Enming
authors.initials: E
authors.lastname: Hu
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, P. R. China.
authors.forename: Chao
authors.initials: C
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Information System of Mountainous Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang, P. R. China.|Guizhou Engineering Laboratory for Quality Control & Evaluation Technology of Medicine, Guiyang, P. R. China.|The Research Center for Quality Control of Natural Medicine, Guizhou Normal University, Guiyang, P. R. China.
authors.forename: Xiaojian
authors.initials: X
authors.lastname: Gong
mesh_terms: D018926:Anti-Allergic Agents / Q000302:isolation & purification* / Q000494:pharmacology; D004791:Enzyme Inhibitors / Q000302:isolation & purification / Q000494:pharmacology; D000431:Ethanol; D005419:Flavonoids / Q000302:isolation & purification*; D006821:Hyaluronoglucosaminidase / Q000037:antagonists & inhibitors; D059625:Liquid-Liquid Extraction / Q000379:methods / Q000592:standards; D008722:Methods; D008872:Microwaves; D008962:Models, Theoretical; D031109:Periploca / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D014465:Ultrasonics
chemical_list: D018926:Anti-Allergic Agents; D004791:Enzyme Inhibitors; D005419:Flavonoids; D010936:Plant Extracts; D000431:Ethanol; D006821:Hyaluronoglucosaminidase
keywords: Anti-allergic activity / Microwave-assisted extraction; Periploca forrestii Schltr. flavonoids; Response surface methodology; Ultrasonic pretreatment