title: Cyclic AMP reverses the effects of aging on pacemaker activity and If in sinoatrial node myocytes.
abstract: Aerobic capacity decreases with age, in part because of an age-dependent decline in maximum heart rate (mHR) and a reduction in the intrinsic pacemaker activity of the sinoatrial node of the heart. Isolated sinoatrial node myocytes (SAMs) from aged mice have slower spontaneous action potential (AP) firing rates and a hyperpolarizing shift in the voltage dependence of activation of the "funny current," IfCyclic AMP (cAMP) is a critical modulator of both AP firing rate and Ifin SAMs. Here, we test the ability of endogenous and exogenous cAMP to overcome age-dependent changes in acutely isolated murine SAMs. We found that maximal stimulation of endogenous cAMP with 3-isobutyl-1-methylxanthine (IBMX) and forskolin significantly increased AP firing rate and depolarized the voltage dependence of activation of Ifin SAMs from both young and aged mice. However, these changes were insufficient to overcome the deficits in aged SAMs, and significant age-dependent differences in AP firing rate and Ifpersisted in the presence of IBMX and forskolin. In contrast, the effects of aging on SAMs were completely abolished by a high concentration of exogenous cAMP, which restored AP firing rate and Ifactivation to youthful levels in cells from aged animals. Interestingly, the age-dependent differences in AP firing rates and Ifwere similar in whole-cell and perforated-patch recordings, and the hyperpolarizing shift in Ifpersisted in excised inside-out patches, suggesting a limited role for cAMP in causing these changes. Collectively, the data indicate that aging does not impose an absolute limit on pacemaker activity and that it does not act by simply reducing the concentration of freely diffusible cAMP in SAMs.
authors.affiliation: Department of Physiology and Biophysics, University of Colorado-Anschutz Medical Campus, Aurora, CO 80045.
authors.forename: Emily J
authors.initials: EJ
authors.lastname: Sharpe
authors.affiliation: Department of Physiology and Biophysics, University of Colorado-Anschutz Medical Campus, Aurora, CO 80045.
authors.forename: Eric D
authors.identifier: http://orcid.org/0000-0002-2881-4861
authors.initials: ED
authors.lastname: Larson
authors.affiliation: Department of Physiology and Biophysics, University of Colorado-Anschutz Medical Campus, Aurora, CO 80045 catherine.proenza@ucdenver.edu.|Department of Medicine, Division of Cardiology, University of Colorado-Anschutz Medical Campus, Aurora, CO 80045.
authors.forename: Catherine
authors.identifier: http://orcid.org/0000-0003-4324-6206
authors.initials: C
authors.lastname: Proenza
mesh_terms: D015056:1-Methyl-3-isobutylxanthine / Q000494:pharmacology; D000200:Action Potentials; D000375:Aging / Q000187:drug effects / Q000502:physiology*; D000818:Animals; D002316:Cardiotonic Agents / Q000494:pharmacology; D002478:Cells, Cultured; D005576:Colforsin / Q000494:pharmacology; D000242:Cyclic AMP / Q000378:metabolism* / Q000494:pharmacology; D051379:Mice; D008810:Mice, Inbred C57BL; D032383:Myocytes, Cardiac / Q000187:drug effects / Q000378:metabolism / Q000502:physiology*; D010726:Phosphodiesterase Inhibitors / Q000494:pharmacology; D012849:Sinoatrial Node / Q000166:cytology / Q000254:growth & development / Q000502:physiology*
chemical_list: D002316:Cardiotonic Agents; D010726:Phosphodiesterase Inhibitors; D005576:Colforsin; D000242:Cyclic AMP; D015056:1-Methyl-3-isobutylxanthine