title: Acetylene Fuels TCE Reductive Dechlorination by Defined Dehalococcoides/Pelobacter Consortia.
abstract: Acetylene (C2H2) can be generated in contaminated groundwater sites as a consequence of chemical degradation of trichloroethene (TCE) by in situ minerals, and C2H2is known to inhibit bacterial dechlorination. In this study, we show that while high C2H2(1.3 mM) concentrations reversibly inhibit reductive dechlorination of TCE by Dehalococcoides mccartyi isolates as well as enrichment cultures containing D. mccartyi sp., low C2H2(0.4 mM) concentrations do not inhibit growth or metabolism of D. mccartyi. Cocultures of Pelobacter SFB93, a C2H2-fermenting bacterium, with D. mccartyi strain 195 or with D. mccartyi strain BAV1 were actively sustained by providing acetylene as the electron donor and carbon source while TCE or cis-DCE served as the electron acceptor. Inhibition by acetylene of reductive dechlorination and methanogenesis in the enrichment culture ANAS was observed, and the inhibition was removed by adding Pelobacter SFB93 into the consortium. Transcriptomic analysis of D. mccartyi strain 195 showed genes encoding for reductive dehalogenases (e.g., tceA) were not affected during the C2H2-inhibition, while genes encoding for ATP synthase, biosynthesis, and Hym hydrogenase were down-regulated during C2H2inhibition, consistent with the physiological observation of lower cell yields and reduced dechlorination rates in strain 195. These results will help facilitate the optimization of TCE-bioremediation at contaminated sites containing both TCE and C2H2.
authors.affiliation: Department of Civil and Environmental Engineering, College of Engineering, University of California , Berkeley, California 94720-1710, United States.
authors.forename: Xinwei
authors.identifier: 0000-0002-3070-8929
authors.initials: X
authors.lastname: Mao
authors.affiliation: US Geological Survey , Menlo Park, California 94025, United States.
authors.forename: Ronald S
authors.initials: RS
authors.lastname: Oremland
authors.affiliation: Department of Civil and Environmental Engineering, College of Engineering, University of California , Berkeley, California 94720-1710, United States.
authors.forename: Tong
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Civil and Environmental Engineering, College of Engineering, University of California , Berkeley, California 94720-1710, United States.
authors.forename: Sara
authors.initials: S
authors.lastname: Gushgari
authors.affiliation: Department of Civil and Environmental Engineering, College of Engineering, University of California , Berkeley, California 94720-1710, United States.
authors.forename: Abigail A
authors.initials: AA
authors.lastname: Landers
authors.affiliation: US Geological Survey , Menlo Park, California 94025, United States.
authors.forename: Shaun M
authors.initials: SM
authors.lastname: Baesman
authors.affiliation: Department of Civil and Environmental Engineering, College of Engineering, University of California , Berkeley, California 94720-1710, United States.|Earth and Environmental Sciences Division, Lawrence Berkeley National Laboratory , Berkeley, California 94720, United States.
authors.forename: Lisa
authors.initials: L
authors.lastname: Alvarez-Cohen
mesh_terms: D000114:Acetylene / Q000378:metabolism*; D001673:Biodegradation, Environmental; D041862:Chloroflexi / Q000378:metabolism; D054879:Halogenation; D014241:Trichloroethylene / Q000378:metabolism*
chemical_list: D014241:Trichloroethylene; D000114:Acetylene