title: Cell-Conditioned Protein Coronas on Engineered Particles Influence Immune Responses.
abstract: A protein corona, which forms on engineered particles as soon as they are introduced into biological environments, is known to provide particles with a "biological identity". Protein coronas derived from various biological environments have been demonstrated to alter the cell internalization mechanism, to diminish targeting ability and to induce nanoparticle aggregation. So far, most of these studies have challenged engineered particles with a static biological environment. However, the extracellular environment is highly dynamic due to the process termed "cell-conditioning", in which cells deplete and secrete biomolecules. In this work, we demonstrate that protein coronas formed on engineered particles from such cell-conditioned media affect the biophysical particle properties and protein adsorption differently to protein coronas derived from an unconditioned environment. When investigating particles with protein coronas formed in various biologically relevant environments for their interaction with immune cells, we observed differences in pro-inflammatory cytokine secretion and immune cell apoptosis. We found that the particles either increased or mitigated the secretion of a specific cytokine, depending on the environment where the protein corona was formed. Our study suggests that the use of protein coronas could be useful to engineer drug carriers for elongated circulation, enhanced biocompatibility, and lower toxicity by triggering a specific immune response.
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Dai
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Junling
authors.initials: J
authors.lastname: Guo
authors.affiliation: Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin , Dublin 4, Ireland.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yan
authors.affiliation: Bio21 Molecular Science and Biotechnology Institute, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Ching-Seng
authors.initials: CS
authors.lastname: Ang
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Nadja
authors.initials: N
authors.lastname: Bertleff-Zieschang
authors.affiliation: ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, and the Department of Chemical and Biomolecular Engineering, The University of Melbourne , Parkville, Victoria 3010, Australia.
authors.forename: Frank
authors.identifier: 0000-0002-0197-497X
authors.initials: F
authors.lastname: Caruso
mesh_terms: D017209:Apoptosis; D002478:Cells, Cultured; D016207:Cytokines / Q000378:metabolism; D006801:Humans; D008264:Macrophages / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D009000:Monocytes / Q000276:immunology* / Q000378:metabolism / Q000473:pathology; D053758:Nanoparticles / Q000737:chemistry*; D000066970:Protein Corona / Q000737:chemistry* / Q000378:metabolism
chemical_list: D016207:Cytokines; D000066970:Protein Corona