title: The connection between BRG1, CTCF and topoisomerases at TAD boundaries.
abstract: The eukaryotic genome is partitioned into topologically associating domains (TADs). Despite recent advances characterizing TADs and TAD boundaries, the organization of these structures is an important dimension of genome architecture and function that is not well understood. Recently, we demonstrated that knockdown of BRG1, an ATPase driving the chromatin remodeling activity of mammalian SWI/SNF enzymes, globally alters long-range genomic interactions and results in a reduction of TAD boundary strength. We provided evidence suggesting that this effect may be due to BRG1 affecting nucleosome occupancy around CTCF sites present at TAD boundaries. In this review, we elaborate on our findings and speculate that BRG1 may contribute to the regulation of the structural and functional properties of chromatin at TAD boundaries by affecting the function or the recruitment of CTCF and DNA topoisomerase complexes.
authors.affiliation: a Department of Cell and Developmental Biology , University of Massachusetts Medical School , Worcester , MA , USA.
authors.forename: A Rasim
authors.initials: AR
authors.lastname: Barutcu
authors.affiliation: b Department of Biochemistry , University of Vermont College of Medicine , Burlington , VT , USA.
authors.forename: Jane B
authors.initials: JB
authors.lastname: Lian
authors.affiliation: b Department of Biochemistry , University of Vermont College of Medicine , Burlington , VT , USA.
authors.forename: Janet L
authors.initials: JL
authors.lastname: Stein
authors.affiliation: b Department of Biochemistry , University of Vermont College of Medicine , Burlington , VT , USA.
authors.forename: Gary S
authors.initials: GS
authors.lastname: Stein
authors.affiliation: a Department of Cell and Developmental Biology , University of Massachusetts Medical School , Worcester , MA , USA.
authors.forename: Anthony N
authors.initials: AN
authors.lastname: Imbalzano
mesh_terms: D000251:Adenosine Triphosphatases / Q000378:metabolism*; D000818:Animals; D000076246:CCCTC-Binding Factor; D026942:DNA Topoisomerases / Q000378:metabolism*; D023281:Genomics; D006801:Humans; D009707:Nucleosomes / Q000378:metabolism; D012097:Repressor Proteins / Q000378:metabolism*
chemical_list: D000076246:CCCTC-Binding Factor; C000619697:CTCF protein, human; D009707:Nucleosomes; D012097:Repressor Proteins; D000251:Adenosine Triphosphatases; D026942:DNA Topoisomerases
keywords: BRG1; CTCF; Hi-C; SWI/SNF;TADs; topoisomerase; topologically associated domains