title: Probing Conformational Exchange Dynamics in a Short-Lived Protein Folding Intermediate by Real-Time Relaxation-Dispersion NMR.
abstract: NMR spectroscopy is a powerful tool for studying molecular dynamics at atomic resolution simultaneously for a large number of nuclear sites. In this communication, we combine two powerful NMR techniques, relaxation-dispersion NMR and real-time NMR, in order to obtain unprecedented information on the conformational exchange dynamics present in short-lived excited protein states, such as those transiently accumulated during protein folding. We demonstrate the feasibility of the approach for the amyloidogenic protein β2-microglobulin that folds via an intermediate state which is believed to be responsible for the onset of the aggregation process leading to amyloid formation.
authors.affiliation: Institut de Biologie Structurale, Université Grenoble Alpes , 71 Avenue des Martyrs, 38044 Grenoble Cedex 9, France.|Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Grenoble, France.|Centre National de Recherche Scientifique (CNRS), Grenoble, France.
authors.forename: Rémi
authors.initials: R
authors.lastname: Franco
authors.affiliation: Instituto de Investigaciones Químicas (CSIC/US), Sevilla, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Gil-Caballero
authors.affiliation: Institut de Biologie Structurale, Université Grenoble Alpes , 71 Avenue des Martyrs, 38044 Grenoble Cedex 9, France.|Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Grenoble, France.|Centre National de Recherche Scientifique (CNRS), Grenoble, France.
authors.forename: Isabel
authors.initials: I
authors.lastname: Ayala
authors.affiliation: Institut de Biologie Structurale, Université Grenoble Alpes , 71 Avenue des Martyrs, 38044 Grenoble Cedex 9, France.|Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Grenoble, France.|Centre National de Recherche Scientifique (CNRS), Grenoble, France.
authors.forename: Adrien
authors.initials: A
authors.lastname: Favier
authors.affiliation: Institut de Biologie Structurale, Université Grenoble Alpes , 71 Avenue des Martyrs, 38044 Grenoble Cedex 9, France.|Commissariat à l'Energie Atomique et aux Energies Alternatives (CEA), Grenoble, France.|Centre National de Recherche Scientifique (CNRS), Grenoble, France.
authors.forename: Bernhard
authors.identifier: 0000-0001-7652-7384
authors.initials: B
authors.lastname: Brutscher
mesh_terms: D006801:Humans; D056004:Molecular Dynamics Simulation; D019906:Nuclear Magnetic Resonance, Biomolecular; D011487:Protein Conformation; D017510:Protein Folding; D013997:Time Factors; D001613:beta 2-Microglobulin / Q000737:chemistry*
chemical_list: D001613:beta 2-Microglobulin