title: Detection of Anti-LeptospiraIgM Antibody in Serum Samples of Suspected Patients Visiting National Public Health Laboratory, Teku, Kathmandu.
abstract: Leptospirosis is a globally distributed zoonosis with varied clinical outcomes and multiorgan involvement in humans. In this study conducted from July 2011 to December 2011, 178 serum samples from patients suspected of leptospirosis were tested by Panbio IgM ELISA at National Public Health Laboratory, Kathmandu, out of which 51 (28.65%) were positive for anti-LeptospiraIgM antibody. Leptospirosis was more common in people in their 2nd and 3rd decades of their life which together comprised 56.86% of the total positive cases. Most of those tested positive were farmers followed by students and housewives. Both animal contact and water contact seemed to play significant roles in disease transmission. Symptoms were vague with the most common being fever, headache, myalgia, abdominal pain, vomiting, jaundice, and diarrhoea. Life style heavily dominated by agronomical and farming activities in Nepal is conducive to leptospirosis transmission. Leptospirosis seems to be a significant public health problem in Nepal but is underestimated. In resource poor countries like Nepal where laboratories performing MAT or maintaining cultures are rarely available, serological test like ELISA could well depict the scenario of the disease prevalence.
authors.affiliation: Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal.
authors.forename: Krishna Prasad
authors.identifier: 0000-0002-5543-846X
authors.initials: KP
authors.lastname: Dahal
authors.affiliation: Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal.
authors.forename: Supriya
authors.initials: S
authors.lastname: Sharma
authors.affiliation: Tribhuvan University Teaching Hospital, Kathmandu, Nepal.
authors.forename: Jeevan Bahadur
authors.initials: JB
authors.lastname: Sherchand
authors.affiliation: National Public Health Laboratory, Teku, Nepal.
authors.forename: Bishnu Prasad
authors.initials: BP
authors.lastname: Upadhyay
authors.affiliation: Central Department of Microbiology, Tribhuvan University, Kirtipur, Nepal.
authors.forename: Dwij Raj
authors.initials: DR
authors.lastname: Bhatta