title: Development of immersion vaccine for bacterial cold-water disease in ayu Plecoglossus altivelis.
abstract: Flavobacterium psychrophilum (F. psychrophilum) is the causative agent of bacterial cold-water disease (BCWD) that occurs in ayu Plecoglossus altivelis. Formalin-killed cell of F. psychrophilum has long been studied as an immersion vaccine for BCWD. In this study, we explored the possibility of F. psychrophilum collagenase (fpcol) for use as the immersion vaccine. BCWD convalescent ayu sera contained specific IgM antibodies against somatic F. psychrophilum and fpcol, meaning that fpcol is a promising antigen for the vaccine development. The recombinant fpcol was successfully expressed in Escherichia coli and Brevibacillus chosinensis (B. chosinensis). The culture supernatant of the B. chosinensis was used as an immersion vaccine solution. The vaccinated ayu were then challenged by soaking into F. psychrophilum culture. In two experimental groups, the relative percentages of survivals were 63 and 38%, respectively, suggesting that fpcol is promising as the immersion vaccine for ayu-BCWD.
authors.affiliation: a Department of Freshwater Fisheries , Wakayama Prefectural Fisheries Experimental Station , Kinokawa-shi , Japan.
authors.forename: Hitoshi
authors.initials: H
authors.lastname: Nakayama
authors.affiliation: b Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
authors.forename: Mariko
authors.initials: M
authors.lastname: Mori
authors.affiliation: b Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
authors.forename: Teisuke
authors.initials: T
authors.lastname: Takita
authors.affiliation: b Division of Food Science and Biotechnology, Graduate School of Agriculture , Kyoto University , Kyoto , Japan.
authors.forename: Kiyoshi
authors.initials: K
authors.lastname: Yasukawa
authors.affiliation: c Nippi Research Institute of Biomatrix , Toride-shi , Japan.
authors.forename: Keisuke
authors.initials: K
authors.lastname: Tanaka
authors.affiliation: c Nippi Research Institute of Biomatrix , Toride-shi , Japan.
authors.forename: Shunji
authors.initials: S
authors.lastname: Hattori
authors.affiliation: d Kanagawa Prefectural Fisheries Technology Center Freshwater Experiment Station , Sagamihara-shi , Japan.
authors.forename: Hideaki
authors.initials: H
authors.lastname: Aikawa
authors.affiliation: d Kanagawa Prefectural Fisheries Technology Center Freshwater Experiment Station , Sagamihara-shi , Japan.
authors.forename: Osamu
authors.initials: O
authors.lastname: Hasegawa
authors.affiliation: e Shiga Prefectural Fisheries Experiment Station , Hikone-shi , Japan.
authors.forename: Takashi
authors.initials: T
authors.lastname: Okamura
authors.affiliation: e Shiga Prefectural Fisheries Experiment Station , Hikone-shi , Japan.
authors.forename: Kentarou
authors.initials: K
authors.lastname: Takegami
authors.affiliation: f Matsuken Pharmaceutical Industry Co., Ltd. , Koganei-shi , Japan.
authors.forename: Shogo
authors.initials: S
authors.lastname: Motokawa
authors.affiliation: f Matsuken Pharmaceutical Industry Co., Ltd. , Koganei-shi , Japan.
authors.forename: Masakazu
authors.initials: M
authors.lastname: Kuwahara
authors.affiliation: f Matsuken Pharmaceutical Industry Co., Ltd. , Koganei-shi , Japan.
authors.forename: Kenichi
authors.initials: K
authors.lastname: Amano
mesh_terms: D000818:Animals; D017756:Aquaculture; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology; D001428:Bacterial Vaccines / Q000235:genetics / Q000276:immunology / Q000494:pharmacology*; D058675:Brevibacillus / Q000235:genetics; D017364:Collagenases / Q000235:genetics / Q000276:immunology; D004926:Escherichia coli / Q000235:genetics; D005393:Fish Diseases / Q000382:microbiology* / Q000517:prevention & control; D045826:Flavobacteriaceae Infections / Q000517:prevention & control / Q000662:veterinary*; D005417:Flavobacterium / Q000276:immunology* / Q000472:pathogenicity; D014614:Vaccines, Synthetic / Q000235:genetics / Q000276:immunology / Q000494:pharmacology
chemical_list: D001426:Bacterial Proteins; D001428:Bacterial Vaccines; D014614:Vaccines, Synthetic; D017364:Collagenases
keywords: Flavobacterium psychrophilum; ayu Plecoglossus altivelis; bacterial cold-water disease; collagenase; immersion vaccine