title: Experimental pathogenicity and complete genome characterization of a pig origin Pasteurella multocida serogroup F isolate HN07.
abstract: Pasteurella multocida serotype F isolates are predominately prevalent in avian hosts, but rarely seen in pigs. However, we isolated several strains of P. multocida serotype F from clinical samples of pigs in China. To understand the pathogenicity of these strains, one of the serotype F isolates designated HN07, was used to challenge experimental chickens, as P. multocida of this serotype is predominately prevalent in avian hosts. However, strain HN07 could not resulted in significant clinical signs in experimental chickens even at an infective dose of ∼109CFU, suggesting the isolate was avirulent to chickens and therefore raising the possibility that the porcine serotype F isolate is not transmitted by chickens. We then used HN07 to challenge experimental pigs, as this strain was isolated from pigs. As expected, the strain led to the clinical signs and the pathological lesions in experimental pigs that are similar to the pasteurellosis disease. We then determined the complete genome sequence of the pig origin serogroup F isolate HN07 for the first time. Genome comparison between HN07 and the avian serotype F P. multocida Pm70 identified a novel integrative conjugative element (ICE) ICEpmcn07 which was likely to harbor a series of genes responsible for a putative type IV secretion system (T4SS) in HN07. This is the first time that we determined an ICE carrying a T4SS in P. multocida. Besides, comparative analysis also defined a number of virulence-associated genes in HN07 but absent in Pm70 which may have a contribution to the pathogenicity of the strain. This is the first report of the pathogenicity and genome characterization of a pig origin Pasteurella multocida serogroup F isolate. The pathogenic and genomic definition of the pig origin P. multocida serogroup F in our study would have significance on the pathogenesis and genetic diversity and virulence variability of P. multocida.
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Peng
authors.affiliation: The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China; Key Laboratory of Animal Genetics, Breeding and Reproduction, Ministry of Education, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Wan
authors.initials: W
authors.lastname: Liang
authors.affiliation: Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, United States.
authors.forename: Yuanguo
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Wenjing
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Hongfeng
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Teng
authors.initials: T
authors.lastname: Yu
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Anding
authors.initials: A
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China.
authors.forename: Huanchun
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Agricultural Microbiology, College of Animal Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei 430070, China; The Cooperative Innovation Center for Sustainable Pig Production, Huazhong Agricultural University, Wuhan, Hubei 430070, China. Electronic address: wub@mail.hzau.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Wu
mesh_terms: D000818:Animals; D002645:Chickens / Q000382:microbiology; D005798:Genes, Bacterial / Q000235:genetics; D016680:Genome, Bacterial / Q000235:genetics*; D010326:Pasteurella Infections / Q000382:microbiology / Q000473:pathology / Q000662:veterinary*; D016979:Pasteurella multocida / Q000145:classification / Q000235:genetics* / Q000472:pathogenicity*; D020641:Polymorphism, Single Nucleotide; D065288:Serogroup; D013045:Species Specificity; D013047:Specific Pathogen-Free Organisms; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology* / Q000473:pathology; D014774:Virulence / Q000235:genetics
keywords: Genome characterization; Pasteurella multocida; Pathogenicity; Pig origin; Serogroup F