title: Ecogenomic responses of benthic communities under multiple stressors along the marine and adjacent riverine areas of northern Bohai Sea, China.
abstract: Benthic communities in the aquatic ecosystem are influenced by both natural and anthropogenic stressors. To understand the ecogenomic responses of sediment communities to the multiple stressors of polluted environments, the bacteria, protistan and metazoan communities in sediments from marine and adjacent riverine areas of North Bohai Sea were characterized by environmental DNA meta-systematics, and their associations with environmental variables were assessed by multiple statistical approaches. The bacterial communities were dominated by Firmicutes (mean 22.4%), Proteobacteria (mean 21.6%) and Actinobacteria (mean 21.5%). The protistan communities were dominated by Ochrophyta (33.7%), Cercozoa (18.9%) and Ciliophora (17.9%). Arthropoda (71.1%) dominated the metazoan communities in sediments. The structures of communities in sediments were shaped by both natural variables (spatial variability and/or salinity (presented as Na and Ca)) and anthropogenic contaminants, including DDTs, PAHs or metals (Cu, Al, Co, Cr, Cu, Fe, K, Mg, Mn, Ni and Zn). Particularly, the correlation network of multiple communities was modulated by the concentrations of Na and DDTs at the family level. Overall, environmental DNA meta-systematics can provide a powerful tool for biomonitoring, sediment quality assessment, and key stressors identification.
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210003, China.
authors.forename: Yuwei
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Department of Ocean Environmental Sciences, Chungnam National University, Daejeon 34134, Republic of Korea.
authors.forename: Seongjin
authors.initials: S
authors.lastname: Hong
authors.affiliation: School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, 08826, Republic of Korea.
authors.forename: Seonjin
authors.initials: S
authors.lastname: Kim
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210003, China. Electronic address: howard50003250@yahoo.com.
authors.forename: Xiaowei
authors.initials: X
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210003, China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210003, China; Department of Veterinary Biomedical Sciences and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada; School of Biological Sciences, University of Hong Kong, Hong Kong; Department of Biology, Hong Kong Baptist University, Hong Kong.
authors.forename: John P
authors.initials: JP
authors.lastname: Giesy
authors.affiliation: State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Tieyu
authors.initials: T
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
authors.forename: Yonglong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: State Key Laboratory of Pollution Control & Resource Reuse, School of the Environment, Nanjing University, Nanjing, 210003, China.
authors.forename: Hongxia
authors.initials: H
authors.lastname: Yu
authors.affiliation: School of Earth and Environmental Sciences & Research Institute of Oceanography, Seoul National University, Seoul, 08826, Republic of Korea. Electronic address: jskocean@snu.ac.kr.
authors.forename: Jong Seong
authors.initials: JS
authors.lastname: Khim
mesh_terms: D000818:Animals; D001181:Arthropods / Q000235:genetics; D002681:China; D003634:DDT / Q000032:analysis; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D017753:Ecosystem; D004784:Environmental Monitoring / Q000379:methods*; D056890:Eukaryota / Q000235:genetics; D000068536:Bacillota / Q000235:genetics; D019015:Geologic Sediments / Q000737:chemistry* / Q000382:microbiology; D008670:Metals / Q000032:analysis; D009792:Oceans and Seas; D010575:Pesticides / Q000032:analysis; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D045483:Rivers / Q000737:chemistry* / Q000382:microbiology; D014871:Water Microbiology; D014874:Water Pollutants, Chemical / Q000032:analysis*
chemical_list: D004269:DNA, Bacterial; D008670:Metals; D010575:Pesticides; D011084:Polycyclic Aromatic Hydrocarbons; D012336:RNA, Ribosomal, 16S; D014874:Water Pollutants, Chemical; D003634:DDT
keywords: Ecogenomics; Metals; Molecular ecology; Next generation sequencing; Organochlorine pesticide; Sediment quality assessment