title: Purification and differentiation of human adipose-derived stem cells by membrane filtration and membrane migration methods.
abstract: Human adipose-derived stem cells (hADSCs) are easily isolated from fat tissue without ethical concerns, but differ in purity, pluripotency, differentiation ability, and stem cell marker expression, depending on the isolation method. We isolated hADSCs from a primary fat tissue solution using: (1) conventional culture, (2) a membrane filtration method, (3) a membrane migration method where the primary cell solution was permeated through membranes, adhered hADSCs were cultured, and hADSCs migrated out from the membranes. Expression of mesenchymal stem cell markers and pluripotency genes, and osteogenic differentiation were compared for hADSCs isolated by different methods using nylon mesh filter membranes with pore sizes ranging from 11 to 80 μm. hADSCs isolated by the membrane migration method had the highest MSC surface marker expression and efficient differentiation into osteoblasts. Osteogenic differentiation ability of hADSCs and MSC surface marker expression were correlated, but osteogenic differentiation ability and pluripotent gene expression were not.
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan.
authors.forename: Hong Reng
authors.initials: HR
authors.lastname: Lin
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan.
authors.forename: Chao-Wen
authors.initials: CW
authors.lastname: Heish
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan.
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Liu
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan.
authors.forename: Saradaprasan
authors.initials: S
authors.lastname: Muduli
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan.
authors.forename: Hsing-Fen
authors.initials: HF
authors.lastname: Li
authors.affiliation: Department of Chemical and Materials Engineering, National Central University, No. 300, Jhongda RD., Jhongli, Taoyuan 32001, Taiwan.|Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia.|Nano Medical Engineering Laboratory, RIKEN, 2-1, Hirosawa, Wako, Saitama 351-0198, Japan.
authors.forename: Akon
authors.initials: A
authors.lastname: Higuchi
authors.affiliation: Department of Medical Microbiology and Parasitology, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia.
authors.forename: S Suresh
authors.initials: SS
authors.lastname: Kumar
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Abdullah A
authors.initials: AA
authors.lastname: Alarfaj
authors.affiliation: Department of Botany and Microbiology, King Saud University, Riyadh 11451, Saudi Arabia.
authors.forename: Murugan A
authors.initials: MA
authors.lastname: Munusamy
authors.affiliation: Department of Obstetrics and Gynecology, Taiwan Landseed Hospital, 77, Kuangtai Road, Pingjen City, Taoyuan 32405, Taiwan.
authors.forename: Shih-Tien
authors.initials: ST
authors.lastname: Hsu
authors.affiliation: Department of Internal Medicine, Taiwan Landseed Hospital, 77, Kuangtai Road, Pingjen City, Taoyuan 32405, Taiwan.
authors.forename: Da-Chung
authors.initials: DC
authors.lastname: Chen
authors.affiliation: Department of Agriculture, Food and Environment, University of Pisa, Via del Borghetto 80, 56124 Pisa, Italy.
authors.forename: Giovanni
authors.initials: G
authors.lastname: Benelli
authors.affiliation: Division of Entomology, Department of Zoology, School of Life Sciences, Bharathiar University, Coimbatore 641 046, Tamil Nadu, India.|Department of Zoology, Thiruvalluvar University, Serkkadu, Vellore 632 115, India.
authors.forename: Kadarkarai
authors.initials: K
authors.lastname: Murugan
authors.affiliation: Department of Surgery, National Taiwan University Hospital and College of Medicine, 7 Chung-Shan S. Rd., Taipei 100, Taiwan.
authors.forename: Nai-Chen
authors.initials: NC
authors.lastname: Cheng
authors.affiliation: Hungchi Women &Children's Hospital, No. 233, Yuanhua Rd., Jhongli, Taoyuan 320, Taiwan.
authors.forename: Han-Chow
authors.initials: HC
authors.lastname: Wang
authors.affiliation: Graduate Institute of Medical Sciences and Department of Obstetrics &Gynecology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan.
authors.forename: Gwo-Jang
authors.initials: GJ
authors.lastname: Wu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology*; D015415:Biomarkers / Q000032:analysis; D002454:Cell Differentiation; D002465:Cell Movement; D002469:Cell Separation / Q000379:methods*; D005374:Filtration / Q000379:methods*; D006801:Humans; D013234:Stem Cells / Q000502:physiology*
chemical_list: D015415:Biomarkers