title: Spatiotemporal expression of Ski after rat spinal cord injury.
abstract: Ski is an evolutionarily conserved protein and widely participates in the regulation of various pathological and physiological processes such as wound healing, liver regeneration, development of the embryonic nervous system, muscle differentiation, and progression of many kinds of tumors. However, the distribution and function of Ski in central nervous system lesion and disease remain unclear. In this study, we investigated the spatiotemporal expression of Ski in a spinal cord injury (SCI) model in adult rats. Western Blot analysis indicated that Ski was expressed in both normal and injured spinal cord, and showed a significant upregulation after SCI compared with the sham group. Double-labeled immunofluorescence staining showed that Ski was significantly expressed in astrocytes, but not in the neurons. Western Blot analyses of glial fibrillary acidic protein (GFAP) and BBB scores were carried out and correlation analysis showed a positive correlation between them. In addition, the relative expression level of Ski was also positively correlated with the relative expression level of GFAP. Moreover, the conspicuous co-expression band of Ski and GFAP at the lesion border was found in the results of immunofluorescence staining combined with the pattern of glial scar formation reflected by H&E staining; in addition, it was found that Ski was also highly associated with glial scar. On the basis of our data, we speculated that Ski might play an important role in the process of reactive astrogliosis after SCI and our study might provide a basis for further study on the detailed role of Ski in astrocytes.
authors.affiliation: aState Key Laboratory of Trauma, Burns and Combined Injury, The Third Department of Research Institute of Surgery, Daping Hospital, Third Military Medical University, Chongqing bDepartment of Orthopedics, Second Clinical Medical College of Lanzhou University, Lanzhou, Gansu, China.
authors.forename: Kaisheng
authors.initials: K
authors.lastname: Zhou
authors.forename: Wei
authors.initials: W
authors.lastname: Nan
authors.forename: Dongliang
authors.initials: D
authors.lastname: Feng
authors.forename: Zhigang
authors.initials: Z
authors.lastname: Yi
authors.forename: Yandong
authors.initials: Y
authors.lastname: Zhu
authors.forename: Zaiyun
authors.initials: Z
authors.lastname: Long
authors.forename: Sen
authors.initials: S
authors.lastname: Li
authors.forename: Haihong
authors.initials: H
authors.lastname: Zhang
authors.forename: Yamin
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000818:Animals; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation / Q000502:physiology*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D010751:Phosphopyruvate Hydratase / Q000378:metabolism; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D013116:Spinal Cord / Q000378:metabolism*; D013119:Spinal Cord Injuries / Q000378:metabolism* / Q000473:pathology* / Q000503:physiopathology; D013997:Time Factors
chemical_list: D005904:Glial Fibrillary Acidic Protein; D011518:Proto-Oncogene Proteins; C525545:Ski protein, rat; D010751:Phosphopyruvate Hydratase