title: Proteomic Profiling of Neuroblastoma Cells Adhesion on Hyaluronic Acid-Based Surface for Neural Tissue Engineering.
abstract: The microenvironment of neuron cells plays a crucial role in regulating neural development and regeneration. Hyaluronic acid (HA) biomaterial has been applied in a wide range of medical and biological fields and plays important roles in neural regeneration. PC12 cells have been reported to be capable of endogenous NGF synthesis and secretion. The purpose of this research was to assess the effect of HA biomaterial combining with PC12 cells conditioned media (PC12 CM) in neural regeneration. Using SH-SY5Y cells as an experimental model, we found that supporting with PC12 CM enhanced HA function in SH-SY5Y cell proliferation and adhesion. Through RP-nano-UPLC-ESI-MS/MS analyses, we identified increased expression of HSP60 and RanBP2 in SH-SY5Y cells grown on HA-modified surface with cotreatment of PC12 CM. Moreover, we also identified factors that were secreted from PC12 cells and may promote SH-SY5Y cell proliferation and adhesion. Here, we proposed a biomaterial surface enriched with neurotrophic factors for nerve regeneration application.
authors.affiliation: Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Ming-Hui
authors.identifier: 0000-0001-5856-0498
authors.initials: MH
authors.lastname: Yang
authors.affiliation: Department of Pathology, Changhua Christian Hospital, Changhua 500, Taiwan.
authors.forename: Ko-Chin
authors.initials: KC
authors.lastname: Chen
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Pei-Wen
authors.initials: PW
authors.lastname: Chiang
authors.affiliation: Department of Biomedical Engineering, National Yang-Ming University, Taipei 112, Taiwan.
authors.forename: Tze-Wen
authors.identifier: 0000-0002-7469-9913
authors.initials: TW
authors.lastname: Chung
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Wan-Jou
authors.identifier: 0000-0001-5817-8627
authors.initials: WJ
authors.lastname: Chen
authors.affiliation: Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan; Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Pei-Yu
authors.initials: PY
authors.lastname: Chu
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Sharon Chia-Ju
authors.identifier: 0000-0002-9238-7112
authors.initials: SC
authors.lastname: Chen
authors.affiliation: Office of Safety, Health and Environment, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
authors.forename: Yi-Shan
authors.initials: YS
authors.lastname: Lu
authors.affiliation: Mass Spectrometry Laboratory, Chemical, Molecular and Materials Analysis Center, Department of Chemistry, National University of Singapore, Singapore 119077.
authors.forename: Cheng-Hui
authors.initials: CH
authors.lastname: Yuan
authors.affiliation: Department of Biomedical Engineering, Chung Yuan Christian University, Chungli 300, Taiwan.
authors.forename: Ming-Chen
authors.initials: MC
authors.lastname: Wang
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Chia-Yang
authors.initials: CY
authors.lastname: Lin
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Ying-Fong
authors.initials: YF
authors.lastname: Huang
authors.affiliation: Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Nuclear Medicine, Kaohsiung Medical University Hospital, Kaohsiung 807, Taiwan.
authors.forename: Shiang-Bin
authors.initials: SB
authors.lastname: Jong
authors.affiliation: Department of Chemistry, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Po-Chiao
authors.initials: PC
authors.lastname: Lin
authors.affiliation: Center for Infectious Disease and Cancer Research, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan; Institute of Medical Science and Technology, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Yu-Chang
authors.identifier: 0000-0002-1917-5893
authors.initials: YC
authors.lastname: Tyan
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D060833:Cellular Microenvironment / Q000187:drug effects; D018834:Chaperonin 60 / Q000096:biosynthesis; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects; D006801:Humans; D006820:Hyaluronic Acid / Q000008:administration & dosage*; D024101:Mitochondrial Proteins / Q000096:biosynthesis; D018832:Molecular Chaperones / Q000096:biosynthesis; D009416:Nerve Regeneration / Q000235:genetics; D009447:Neuroblastoma / Q000188:drug therapy / Q000378:metabolism* / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000502:physiology; D028861:Nuclear Pore Complex Proteins / Q000096:biosynthesis; D016716:PC12 Cells; D051381:Rats; D023822:Tissue Engineering
chemical_list: D018834:Chaperonin 60; C557067:Hspd1 protein, rat; D024101:Mitochondrial Proteins; D018832:Molecular Chaperones; D028861:Nuclear Pore Complex Proteins; C094493:ran-binding protein 2; D006820:Hyaluronic Acid