title: Hyaluronic Acid Coating Enhances Biocompatibility of Nonwoven PGA Scaffold and Cartilage Formation.
abstract: Synthetic polymers such as polyglycolic acid (PGA) fibers are the traditional tissue engineering scaffolds that are widely used for engineering a variety of soft tissues. However, the major disadvantage of this polymer material is its released acidic degradation products that trigger inflammatory response and fibrotic process, which affects the biocompatibility and the quality of the engineered tissues. In this study, the effect of hyaluronic acid (HA) coating on improving PGA biocompatibility was explored. The results showed that 1% HA solution could better coat PGA fibers than other tested concentrations of HA, and coated PGA exhibited less inflammatory reaction upon in vivo subcutaneous implantation. In vitro characterization demonstrated that HA coating could enhance cell adhesion to the scaffold and reduce gene expression of IL-1, IL-6, IL-8, and α-SMA. It also decreased the acidity of degradation products in vitro. Furthermore, coated PGA could engineer better cartilages in vitro with higher content of total collagen and glycosaminoglycan, as well as higher gene expression levels of collagen II, aggrecan, and Sox9. Collectively, the data indicate that HA coating can significantly enhance the biocompatibility of this traditional scaffold material, which also enhances the quality of engineered tissues.
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, P.R. China .
authors.forename: Xunxun
authors.initials: X
authors.lastname: Lin
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, P.R. China .
authors.forename: Wenbo
authors.initials: W
authors.lastname: Wang
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, P.R. China .|2 National Tissue Engineering Center of China , Shanghai, P.R. China .
authors.forename: Wenjie
authors.initials: W
authors.lastname: Zhang
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, P.R. China .|2 National Tissue Engineering Center of China , Shanghai, P.R. China .
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, P.R. China .|2 National Tissue Engineering Center of China , Shanghai, P.R. China .
authors.forename: Guangdong
authors.initials: G
authors.lastname: Zhou
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, P.R. China .|2 National Tissue Engineering Center of China , Shanghai, P.R. China .
authors.forename: Yilin
authors.initials: Y
authors.lastname: Cao
authors.affiliation: 1 Department of Plastic and Reconstructive Surgery, Shanghai Key Laboratory of Tissue Engineering, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine , Shanghai, P.R. China .|2 National Tissue Engineering Center of China , Shanghai, P.R. China .
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
mesh_terms: D000818:Animals; D001672:Biocompatible Materials / Q000737:chemistry*; D002356:Cartilage / Q000166:cytology*; D002448:Cell Adhesion; D002478:Cells, Cultured; D020219:Chondrogenesis / Q000502:physiology*; D003094:Collagen; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology; D006801:Humans; D006820:Hyaluronic Acid / Q000737:chemistry*; D011100:Polyglycolic Acid / Q000737:chemistry*; D011817:Rabbits; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials; D011100:Polyglycolic Acid; D006820:Hyaluronic Acid; D003094:Collagen
keywords: biocompatibility; cartilage engineering; inflammatory response; polyglycolic acid; scaffold