title: [Effect of calcium channel blockers on primary cultured human urethra scar fibroblasts].
abstract: To investigate effects of verapamil on primary cultured human urethral scar fibroblasts (USFs) and to provide basis for protecting the formation of urethra scar.  Methods: The cell proliferation was evaluated with the cell counting kit (CCK)-8 method after USFs were incubated various verapamil concentrations (50, 100, 150, 200, or 250 μmol/L) or solvent for 12, 24, or 48 h. The protein level of matrix metalloproteinase (MMP) was evaluated with ELISA after cells were incubated with verapamil (100 μmol/L) or solvent (control cells) for 24 h.  Results: The proliferation of USFs was obviously suppressed after verapamil treatment, which was in a dose-dependent and time-dependent manner. Meanwhile, the protein levels of MMP-2 and MMP-9 in the verapamil treatment group increased obviously compared with those of the control groups (P<0.05).  Conclusion: Calcium channel blockers may prevent the excessive formation of urethra scar by inhibiting the proliferation of urethral scar fibroblasts and enhancing the activity of MMP.
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Mingqiang
authors.initials: M
authors.lastname: Zeng
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Junjie
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Huang
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Ruizhi
authors.initials: R
authors.lastname: Xue
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Xuyu
authors.initials: X
authors.lastname: Xiang
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Fanchang
authors.initials: F
authors.lastname: Zeng
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Wang
authors.affiliation: Department of Urology, Xiangya Hospital, Central South University, Changsha 410008, China.
authors.forename: Zhengyan
authors.initials: Z
authors.lastname: Tang
mesh_terms: D002121:Calcium Channel Blockers / Q000494:pharmacology*; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D002921:Cicatrix / Q000517:prevention & control; D005347:Fibroblasts / Q000187:drug effects*; D006801:Humans; D020778:Matrix Metalloproteinase 2 / Q000187:drug effects; D020780:Matrix Metalloproteinase 9 / Q000187:drug effects; D061965:Matrix Metalloproteinase Inhibitors / Q000494:pharmacology; D015854:Up-Regulation / Q000187:drug effects; D014521:Urethra / Q000166:cytology / Q000473:pathology; D014700:Verapamil / Q000494:pharmacology*
chemical_list: D002121:Calcium Channel Blockers; D061965:Matrix Metalloproteinase Inhibitors; D014700:Verapamil; D020778:Matrix Metalloproteinase 2; D020780:Matrix Metalloproteinase 9