title: Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation.
abstract: The recent development of human induced pluripotent stem cells (hiPSCs) proved that mature somatic cells can return to an undifferentiated, pluripotent state. Now, reprogramming is done with various types of adult somatic cells: keratinocytes, urine cells, fibroblasts, etc. Early experiments were usually done with dermal fibroblasts. However, this required an invasive surgical procedure to obtain fibroblasts from the patients. Therefore, suspension cells, such as blood and urine cells, were considered ideal for reprogramming because of the convenience of obtaining the primary cells. Here, we report an efficient protocol for iPSC generation from peripheral blood mononuclear cells (PBMCs). By plating the transduced PBMCs serially to a new, matrix-coated plate using centrifugation, this protocol can easily provide iPSC colonies. This method is also applicable to umbilical cord blood mononuclear cells (CBMCs). This study presents a simple and efficient protocol for the reprogramming of PBMCs and CBMCs.
authors.affiliation: CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea.
authors.forename: Yeri Alice
authors.initials: YA
authors.lastname: Rim
authors.affiliation: CiSTEM Laboratory, Convergent Research Consortium for Immunologic Disease, Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea.
authors.forename: Yoojun
authors.initials: Y
authors.lastname: Nam
authors.affiliation: Division of Rheumatology, Department of Internal Medicine, Seoul St. Marys Hospital, Institute of Medical Science, College of Medicine, The Catholic University of Korea; juji@catholic.ac.kr.
authors.forename: Ji Hyeon
authors.initials: JH
authors.lastname: Ju
mesh_terms: D018929:Cell Culture Techniques / Q000379:methods*; D065150:Cellular Reprogramming; D002498:Centrifugation; D005347:Fibroblasts; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology*; D007963:Leukocytes, Mononuclear / Q000166:cytology*; D029082:Sendai virus