title: Molecular Modeling and Phylogeny of the Krüppel-like Factor 4 (cKLF4) Protein from the Arabian Camel,Camelus dromedarius.
abstract: Krüppel-like factor 4 (KLF4) is a pluripotency transcription factor that helps in generating induced pluripotent stem cells (iPSCs). We sequenced for the first time the full coding sequence ofCamelus dromedariusKLF4 (cKLF4), which is also known as the Arabian camel. Bioinformatics analysis revealed the molecular weight and the isoelectric point of cKLF4 protein to be 53.043 kDa and 8.74, respectively. The predicted cKLF4 protein sequence shows high identity with some other species as follows: 98% with Bactrian camel and 89% with alpaca KLF4 proteins. A three-dimensional (3D) structure was built based on the available crystal structure of theMus musculusKLF4 (mKLF4) of 82 residues (PDB: 2 WBS) and by predicting 400 residues using bioinformatics software. The comparison confirms the presence of the zinc finger domains in cKLF4 protein. Phylogenetic analysis showed that KLF4 from the Arabian camel is grouped with the Bactrian camel, alpaca, cattle, and pig. This study will help in the annotation of KLF4 protein and in generating camel-induced pluripotent stem cells (CiPSCs).
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdullah O
authors.initials: AO
authors.lastname: Alawad
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Sultan N
authors.initials: SN
authors.lastname: Alharbi
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Othman A
authors.initials: OA
authors.lastname: Alhazzaa
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Faisal S
authors.initials: FS
authors.lastname: Alagrafi
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Mohammad N
authors.initials: MN
authors.lastname: Alkhrayef
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Ziyad A
authors.initials: ZA
authors.lastname: Alhamdan
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Abdullah D
authors.initials: AD
authors.lastname: Alenazi
authors.affiliation: National Center for Stem Cell Technology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.; SAAD Research and Development Center, Clinical Research Laboratory and Radiation Oncology, SAAD Specialist Hospital, Al Khobar, Kingdom of Saudi Arabia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Hammad
authors.affiliation: National Center for biotechnology, King Abdulaziz City for Science and Technology (KACST), Riyadh, Kingdom of Saudi Arabia.
authors.forename: Sami A
authors.initials: SA
authors.lastname: Alyahya
authors.affiliation: National Center for Genomic Technology, King Abdulaziz City for Science and Technology, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Hasan A
authors.initials: HA
authors.lastname: AlJohi
authors.affiliation: National Center for Genomic Technology, King Abdulaziz City for Science and Technology, Riyadh, Kingdom of Saudi Arabia.
authors.forename: Ibrahim O
authors.initials: IO
authors.lastname: Alanazi
keywords: 3D structure; Camelus dromedarius; KLF4; camel-induced pluripotent stem cells (CiPSCs); pluripotency; sequencing