title: New-vista in finding antioxidant and anti-inflammatory property of crude protein extract from Sauropus androgynus leaf.
abstract: BACKGROUND
  tudy describes antioxidant and anti-inflammatory properties of Sauropus androgynus leaf dialysed protein extract. Free radicals are implicated for many diseases including diabetes mellitus, arthritis, cancer, ageing etc. In the treatment of these diseases, antioxidant therapy has gained utmost importance. The main objective of the present study was to reveal the antioxidant and anti-inflammatory potentiality from S. androgynus leaf dialysed protein extract.
  
  
  METHODS
  The antioxidant and anti-inflammatory properties of S. androgynus studied using different models viz. hydroxyl radical scavenging, DPPH radical scavenging, reducing power assay, superoxide radical scavenging activity by alkaline DMSO and phosphomolybdenum antioxidant assays and in vitro antiinflammatory activity by inhibition of protein denaturation, membrane stabilisation test models carried out.
  
  
  RESULTS
  Antioxidant activity was estimated in dose dependent manner showed hydroxyl radical (55.62%), DPPH (50%), reducing power (0.286 Abs), alkaline DMSO (72.51%) and phosphomolybdenum (0.198 Abs) activity high at 50 μg/ml concentration compared to standard curcumin (62.31%), curcumin (56.61%), tannic acid (0.54 Abs), curcumin (75.38%) and vitamin E (0.15 Abs) respectively. In-vitro anti-inflammatory activity by hypotonic induced model showed maximum protection (74.17%) compared to standard Acetylsalcylic acid (86.88%) at 100 μg/ml concentration and also in protein denaturation model protected protein denaturation maximum (83.60%) compared to standard Diclofenac (86.82%) at 100 μg/ml concentration respectively.
  
  
  CONCLUSIONS
  The antioxidant property usually studied related to the polyphenols and flavonoids present in the extract but present finding concluded that S. androgynus giving hint even potential proteins can also show responsible action effective against free radical mediated disease.
authors.affiliation: Department of Biochemistry, Yuvaraja&#;s College, University of Mysore, India.
authors.forename: Chakkere Shivamadhu
authors.initials: CS
authors.lastname: Madhu
authors.affiliation: Pristine Laboratories, Kodigehalli Gate, Karnataka, India.
authors.forename: Honnayakanahalli Marichenne Gowda
authors.initials: HM
authors.lastname: Manukumar
authors.affiliation: Department of Biotechnology, Manasagangothri, University of Mysore, India.
authors.forename: Puttalingaiah
authors.initials: P
authors.lastname: Basavaraju
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D001713:Biphenyl Compounds / Q000378:metabolism; D004121:Dimethyl Sulfoxide / Q000378:metabolism; D004910:Erythrocyte Membrane / Q000187:drug effects; D005419:Flavonoids / Q000494:pharmacology; D006801:Humans; D017665:Hydroxyl Radical / Q000378:metabolism; D019684:Magnoliopsida / Q000737:chemistry*; D010636:Phenols / Q000494:pharmacology; D010851:Picrates / Q000378:metabolism; D010936:Plant Extracts / Q000494:pharmacology*; D018515:Plant Leaves / Q000737:chemistry; D010940:Plant Proteins / Q000494:pharmacology*; D059808:Polyphenols / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects; D014675:Vegetables / Q000737:chemistry
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D001713:Biphenyl Compounds; D005419:Flavonoids; D010636:Phenols; D010851:Picrates; D010936:Plant Extracts; D010940:Plant Proteins; D059808:Polyphenols; D017665:Hydroxyl Radical; C004931:1,1-diphenyl-2-picrylhydrazyl; D004121:Dimethyl Sulfoxide
keywords: anti-inflammatory; antioxidant; erythrocyte; lysosomal; neutrophil