title: A Simple PCR-RFLP Method for Genotyping of IFNL4 rs368234815 Polymorphism in Patients With Chronic Hepatitis C.
abstract: BACKGROUND
  The IFNL4 rs368234815 polymorphism plays a prominent role in spontaneous and treatment-induced clearance of hepatitis C virus (HCV) infection. This study aimed to develop a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method for assessment of rs368234815 polymorphism.
  
  
  METHODS
  We genotyped the rs368234815 polymorphism in 87 patients with chronic HCV by PCR sequencing and PCR-RFLP methods, simultaneously.
  
  
  RESULTS
  Genotyping of IFNL4 rs368234815 via PCR-RFLP was concordant with PCR sequencing in all 87 individuals (100%). The analytical sensitivity and specificity of the developed PCR-RFLP method for genotyping of rs368234815 polymorphism were each 100%. Among these patients with chronic HCV, the frequency of rs368234815 TT/TT, TT/ΔG, and ΔG/ΔG were 44.8%, 37.9%, and 17.3%, respectively.
  
  
  CONCLUSIONS
  The PCR-RFLP method that we developed is accurate, rapid, inexpensive, and easy to perform for genotyping of the IFNL4 rs368234815 polymorphism. This method can be used for clinical and research work.
authors.affiliation: Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.|Armin Pathobiology Laboratory, Tehran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Pouryasin
authors.affiliation: Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.|Armin Pathobiology Laboratory, Tehran, Iran.|Middle East Liver Diseases (MELD) Center, Tehran, Iran.
authors.forename: Heidar
authors.initials: H
authors.lastname: Sharafi
authors.affiliation: Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.|Middle East Liver Diseases (MELD) Center, Tehran, Iran.
authors.forename: Bita
authors.initials: B
authors.lastname: Behnava
authors.affiliation: Baqiyatallah Research Center for Gastroenterology and Liver Diseases, Baqiyatallah University of Medical Sciences, Tehran, Iran.|Middle East Liver Diseases (MELD) Center, Tehran, Iran.
authors.forename: Seyed Moayed
authors.initials: SM
authors.lastname: Alavian
authors.affiliation: Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Keshvari
authors.affiliation: Armin Pathobiology Laboratory, Tehran, Iran, ali.pouryasin@iaua.ac.ir.|Department of Biology, Arsanjan Branch, Islamic Azad University, Arsanjan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Pouryasin
mesh_terms: D000328:Adult; D015331:Cohort Studies; D005260:Female; D060005:Genotyping Techniques / Q000379:methods*; D019698:Hepatitis C, Chronic / Q000235:genetics*; D006801:Humans; D007378:Interleukins / Q000235:genetics*; D008297:Male; D016133:Polymerase Chain Reaction / Q000379:methods*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D055815:Young Adult; D000095022:Interferon Lambda
chemical_list: D007378:Interleukins; C579163:IFNL4 protein, human; D000095022:Interferon Lambda
keywords: Polymerase Chain Reaction; genetic polymorphism; hepatitis C; human IFNL4 protein; restriction fragment length polymorphism