title: Iron oxide nanoparticles modulate heat shock proteins and organ specific markers expression in mice male accessory organs.
abstract: With increased industrial utilization of iron oxide nanoparticles (Fe2O3-NPs), concerns on adverse reproductive health effects following exposure have been immensely raised. In the present study, the effects of Fe2O3-NPs exposure in the seminal vesicle and prostate gland were studied in mice. Mice were exposed to two different doses (25 and 50 mg/kg) of Fe2O3-NPs along with the control and analyzed the expressions of heat shock proteins (HSP60, HSP70 and HSP90) and organ specific markers (Caltrin, PSP94, and SSLP1). Fe2O3-NPs decreased food consumption, water intake, and organo-somatic index in mice with elevated iron levels in serum, urine, fecal matter, seminal vesicle and prostate gland. FTIR spectra revealed alterations in the functional groups of biomolecules on Fe2O3-NPs treatment. These changes are accompanied by increased lactate dehydrogenase levels with decreased total protein and fructose levels. The investigation of oxidative stress biomarkers demonstrated a significant increase in reactive oxygen species, nitric oxide, lipid peroxidation, protein carbonyl content and glutathione peroxidase with a concomitant decrement in the glutathione and ascorbic acid in the male accessory organs which confirmed the induction of oxidative stress. An increase in NADPH-oxidase-4 with a decrease in glutathione-S-transferase was observed in the seminal vesicle and prostate gland of the treated groups. An alteration in HSP60, HSP70, HSP90, Caltrin, PSP94, and SSLP1 expression was also observed. Moreover, accumulation of Fe2O3-NPs brought pathological changes in the seminal vesicle and prostate gland of treated mice. These findings provide evidence that Fe2O3-NPs could be an environmental risk factor for reproductive disease.
authors.affiliation: Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore - 641 046, Tamilnadu, INDIA.
authors.forename: Kiruthika
authors.initials: K
authors.lastname: Sundarraj
authors.affiliation: Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore - 641 046, Tamilnadu, INDIA.
authors.forename: Azhwar
authors.initials: A
authors.lastname: Raghunath
authors.affiliation: Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore - 641 046, Tamilnadu, INDIA.
authors.forename: Lakshmikanthan
authors.initials: L
authors.lastname: Panneerselvam
authors.affiliation: Molecular Toxicology Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore - 641 046, Tamilnadu, INDIA. Electronic address: ekas2009@buc.edu.in.
authors.forename: Ekambaram
authors.initials: E
authors.lastname: Perumal
mesh_terms: D000818:Animals; D004305:Dose-Response Relationship, Drug; D005290:Ferric Compounds / Q000633:toxicity*; D005786:Gene Expression Regulation; D006360:Heat-Shock Proteins / Q000096:biosynthesis* / Q000235:genetics; D008297:Male; D053768:Metal Nanoparticles / Q000633:toxicity*; D051379:Mice; D011467:Prostate / Q000187:drug effects / Q000378:metabolism*; D029584:Prostatic Secretory Proteins / Q000096:biosynthesis* / Q000235:genetics; D011897:Random Allocation; D012669:Seminal Vesicles / Q000187:drug effects / Q000378:metabolism*; D014961:X-Ray Diffraction
chemical_list: D005290:Ferric Compounds; D006360:Heat-Shock Proteins; D029584:Prostatic Secretory Proteins; C000499:ferric oxide
keywords: heat shock proteins; iron accumulation; iron oxide nanoparticles; organ specific markers; prostate gland; seminal vesicle