title: Protein Interactions during the Flavivirus and Hepacivirus Life Cycle.
abstract: Protein-protein interactions govern biological functions in cells, in the extracellular milieu, and at the border between cells and extracellular space. Viruses are small intracellular parasites and thus rely on protein interactions to produce progeny inside host cells and to spread from cell to cell. Usage of host proteins by viruses can have severe consequencese.g.apoptosis, metabolic disequilibria, or altered cell proliferation and mobility. Understanding protein interactions during virus infection can thus educate us on viral infection and pathogenesis mechanisms. Moreover, it has led to important clinical translations, including the development of new therapeutic and vaccination strategies. Here, we will discuss protein interactions of members of theFlaviviridaefamily, which are small enveloped RNA viruses. Dengue virus, Zika virus and hepatitis C virus belong to the most prominent human pathogenicFlaviviridaeWith a genome of roughly ten kilobases encoding only ten viral proteins,Flaviviridaedisplay intricate mechanisms to engage the host cell machinery for their purpose. In this review, we will highlight how dengue virus, hepatitis C virus, Japanese encephalitis virus, tick-borne encephalitis virus, West Nile virus, yellow fever virus, and Zika virus proteins engage host proteins and how this knowledge helps elucidateFlaviviridaeinfection. We will specifically address the protein composition of the virus particle as well as the protein interactions during virus entry, replication, particle assembly, and release from the host cell. Finally, we will give a perspective on future challenges inFlaviviridaeinteraction proteomics and why we believe these challenges should be met.
authors.affiliation: From the Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany gisa.gerold@twincore.de.
authors.forename: Gisa
authors.identifier: 0000-0002-1326-5038
authors.initials: G
authors.lastname: Gerold
authors.affiliation: From the Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
authors.forename: Janina
authors.initials: J
authors.lastname: Bruening
authors.affiliation: From the Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
authors.forename: Bettina
authors.initials: B
authors.lastname: Weigel
authors.affiliation: From the Institute for Experimental Virology, TWINCORE, Centre for Experimental and Clinical Infection Research, a joint venture between the Medical School Hannover and the Helmholtz Centre for Infection Research, Hannover, Germany.
authors.forename: Thomas
authors.initials: T
authors.lastname: Pietschmann
mesh_terms: D005416:Flavivirus / Q000378:metabolism / Q000502:physiology*; D018177:Flavivirus Infections / Q000821:virology*; D016679:Genome, Viral; D016174:Hepacivirus / Q000378:metabolism / Q000502:physiology*; D006526:Hepatitis C / Q000821:virology*; D054884:Host-Pathogen Interactions; D006801:Humans; D060066:Protein Interaction Maps; D053586:Virus Internalization; D014779:Virus Replication