title: High expression of miR-105-1 positively correlates with clinical prognosis of hepatocellular carcinoma by targeting oncogene NCOA1.
abstract: Increasing evidence supports that microRNA (miRNA) plays a significant functional role in cancer progression by directly regulating respective targets. In this study, the expression levels of miR-105-1 and its target gene were analyzed using genes microarray and hierarchical clustering analysis followed by validation with quantitative RT-PCR in hepatocellular carcinoma (HCC) and normal liver tissues. We examined the expression of nuclear receptor coactivator 1 (NCOA1), the potential target gene of miR-105-1, following the transfection of miR-105-1 mimics or inhibitors. Our results showed that miR-105-1 was downregulated in HCC tissues when compared with normal liver tissues and patients with lower miR-105-1 expression had shorter overall survival (OS) and progression free survival (PFS). Moreover, NCOA1 was confirmed to be a direct target of miR-105-1. Furthermore, concomitant high expression of NCOA1 and low expression of miR-105-1 correlated with a shorter median OS and PFS in HCC patients. In conclusion, our results provide the first evidence that NCOA1 is a direct target of miR-105-1 suggesting that NCOA1 and miR-105-1 may have potential prognostic value and may be useful as tumor biomarkers for the diagnosis of HCC patients.
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.|Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China.
authors.forename: Yu-Shui
authors.initials: YS
authors.lastname: Ma
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.|Department of Radiology, The Fourth Affiliated Hospital, Medical University of Anhui, Hefei 230601, China.
authors.forename: Ting-Miao
authors.initials: TM
authors.lastname: Wu
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Zhong-Wei
authors.initials: ZW
authors.lastname: Lv
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Gai-Xia
authors.initials: GX
authors.lastname: Lu
authors.affiliation: Tissue Bank, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors.forename: Xian-Ling
authors.initials: XL
authors.lastname: Cong
authors.affiliation: Department of Pathology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Ru-Ting
authors.initials: RT
authors.lastname: Xie
authors.affiliation: Department of Pathology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Hui-Qiong
authors.initials: HQ
authors.lastname: Yang
authors.affiliation: Department of Pathology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Zheng-Yan
authors.initials: ZY
authors.lastname: Chang
authors.affiliation: Tissue Bank, China-Japan Union Hospital, Jilin University, Changchun 130033, China.
authors.forename: Ran
authors.initials: R
authors.lastname: Sun
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Li
authors.initials: L
authors.lastname: Chai
authors.affiliation: Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Ming-Xiang
authors.initials: MX
authors.lastname: Cai
authors.affiliation: Department of Medical Oncology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Zhong
authors.affiliation: Department of Digestive Surgery, Rui Jin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhu
authors.affiliation: Central Laboratory for Medical Research, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, China.
authors.forename: Da
authors.initials: D
authors.lastname: Fu
mesh_terms: D014408:Biomarkers, Tumor / Q000096:biosynthesis / Q000235:genetics / Q000378:metabolism; D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000502:physiology; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis* / Q000235:genetics / Q000378:metabolism; D056920:Nuclear Receptor Coactivator 1 / Q000235:genetics* / Q000378:metabolism; D011379:Prognosis; D015398:Signal Transduction; D014162:Transfection
chemical_list: D014408:Biomarkers, Tumor; C588223:MIRN105 microRNA, human; D035683:MicroRNAs; C538859:NCOA1 protein, human; D056920:Nuclear Receptor Coactivator 1
keywords: HCC; biomarker; miR-105-1; survival; target