title: The interaction of dietary isoflavones and estradiol replacement on behavior and brain-derived neurotrophic factor in the ovariectomized rat.
abstract: Phytoestrogens are plant derived, non-steroidal compounds naturally found in rodent chows that potentially have endocrine-disrupting effects. Isoflavones, the most common phytoestrogens, have a similar structure and molecular weight to 17β-estradiol (E2) and have the ability to bind and activate both isoforms of the estrogen receptor (ER). Most isoflavones have a higher affinity for ERβ, which is involved in sexually dimorphic behavioral regulation. The goal of this study was to examine the interaction of isoflavones and E2 presence in the OVX rat on anxiety- and depressive- like behavior and the related BDNF pathophysiology. E2 administration resulted in anxiogenic behaviors when isoflavones were present in the diet (p<0.05), but anxiolytic behaviors when isoflavones were not present (p<0.05). E2 resulted in antidepressive-like behaviors in animals fed an isoflavone-rich diet (p<0.05), with no effect when isoflavones were removed. Increased hippocampal BDNF expression was observed in animals fed an isoflavone-rich diet after E2 administration (p<0.05). BDNF expression in the amygdala and hypothalamus was increased after E2 treatment in animals fed an isoflavone-rich diet. Overall, these results demonstrate that the presence of dietary isoflavones can differentially regulate the effect of E2 replacement on behavior and BDNF expression.
authors.affiliation: Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, MD, United States; Center for Neuroscience and Regenerative Medicine, Bethesda, MD, United States.
authors.forename: Ashley L
authors.initials: AL
authors.lastname: Russell
authors.affiliation: Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, MD, United States; Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, United States.
authors.forename: Jamie Moran
authors.initials: JM
authors.lastname: Grimes
authors.affiliation: Center for Neuroscience and Regenerative Medicine, Bethesda, MD, United States.
authors.forename: Darwin O
authors.initials: DO
authors.lastname: Larco
authors.affiliation: Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, United States.
authors.forename: Danette F
authors.initials: DF
authors.lastname: Cruthirds
authors.affiliation: Armed Forces Radiobiology Research Institute, Bethesda, MD, United States.
authors.forename: Joanna
authors.initials: J
authors.lastname: Westerfield
authors.affiliation: Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, United States.
authors.forename: Lawren
authors.initials: L
authors.lastname: Wooten
authors.affiliation: Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, United States.
authors.forename: Margaret
authors.initials: M
authors.lastname: Keil
authors.affiliation: Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Weiser
authors.affiliation: Armed Forces Radiobiology Research Institute, Bethesda, MD, United States.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Landauer
authors.affiliation: Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, United States.
authors.forename: Robert J
authors.initials: RJ
authors.lastname: Handa
authors.affiliation: Program in Neuroscience, Uniformed Services University of the Health Sciences, Bethesda, MD, United States; Center for Neuroscience and Regenerative Medicine, Bethesda, MD, United States; Department of Obstetrics and Gynecology, Uniformed Services University of the Health Sciences, Bethesda, MD, United States. Electronic address: tao-yiao.wu@usuhs.edu.
authors.forename: T John
authors.initials: TJ
authors.lastname: Wu
mesh_terms: D000818:Animals; D014151:Anti-Anxiety Agents / Q000494:pharmacology; D000928:Antidepressive Agents / Q000494:pharmacology; D001007:Anxiety / Q000523:psychology; D001522:Behavior, Animal / Q000187:drug effects*; D001921:Brain / Q000187:drug effects* / Q000378:metabolism; D019208:Brain-Derived Neurotrophic Factor / Q000378:metabolism*; D003863:Depression / Q000523:psychology; D004032:Diet; D004958:Estradiol / Q000009:adverse effects / Q000494:pharmacology*; D005260:Female; D018565:Food-Drug Interactions; D007529:Isoflavones / Q000008:administration & dosage*; D018782:Maze Learning / Q000187:drug effects; D009043:Motor Activity / Q000187:drug effects; D010052:Ovariectomy; D017207:Rats, Sprague-Dawley
chemical_list: D014151:Anti-Anxiety Agents; D000928:Antidepressive Agents; D019208:Brain-Derived Neurotrophic Factor; D007529:Isoflavones; D004958:Estradiol
keywords: 17β-estradiol; Anxiety; BDNF; Depression; Isoflavones