title: In VitroEvaluation of the Antimicrobial Ability and Cytotoxicity on Two Melanoma Cell Lines of a Benzylamide Derivative of Maslinic Acid.
abstract: Maslinic acid is a pentacyclic triterpene extracted from olives that has been systematically reported to exert several therapeutic effects, such as antitumoral, antidiabetic, antioxidant, anti-inflammatory, antiparasitic, and antiviral properties. Recently, new derivatives of maslinic acid have been obtained and expanded the spectrum of biological activities and improved the existing ones. The present study was meant to perform thein vitroassessment of the (i) cytotoxic effects of a benzylamide derivative of maslinic acid ("EM2") (benzyl (2α, 3β) 2,3-diacetoxy-olean-12-en-28-amide) on B164A5 murine melanoma and A375 human malignant melanoma cell lines and the (ii) antimicrobial activity of the compound on several bacterial strains, respectively. We obtained a dose-dependent cytotoxic effect of EM2 that was particularly relevant to the murine cell line. As on the antibacterial activity, EM2 was tested on 10 bacterial strainsBacillus cereus, Staphylococcus aureus,Streptococcus pyogenes, Streptococcus pneumoniae,Enterococcus faecalis,Escherichia coli,Yersinia enterocolitica,Klebsiella pneumoniae,Proteus mirabilis, andPseudomonas aeruginosaand one fungusCandida albicans. A significant antimicrobial effect was recorded forStreptococcus pyogenesandStaphylococcus aureus.
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, "Victor Babeş" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timişoara, Romania.
authors.forename: Ioana Zinuca
authors.identifier: 0000-0002-2223-5228
authors.initials: IZ
authors.lastname: Pavel
authors.affiliation: Department of Pharmacognosy, Faculty of Pharmacy, "Victor Babeş" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timişoara, Romania.
authors.forename: Corina
authors.identifier: 0000-0001-5999-1214
authors.initials: C
authors.lastname: Danciu
authors.affiliation: Department of Pharmaceutical Chemistry, Faculty of Pharmacy, "Victor Babeş" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timişoara, Romania.
authors.forename: Camelia
authors.identifier: 0000-0003-0862-7791
authors.initials: C
authors.lastname: Oprean
authors.affiliation: Department of Toxicology, Faculty of Pharmacy, "Victor Babeş" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timişoara, Romania.
authors.forename: Cristina Adriana
authors.initials: CA
authors.lastname: Dehelean
authors.affiliation: Department of Microbiology and Virology, Faculty of Medicine, "Victor Babeş" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timişoara, Romania.
authors.forename: Delia
authors.initials: D
authors.lastname: Muntean
authors.affiliation: Department of Organic Chemistry, Martin-Luther University Halle-Wittenberg, Kurt-Mothes Str. 2, D-06120 Halle (Saale), Germany.
authors.forename: René
authors.initials: R
authors.lastname: Csuk
authors.affiliation: Department of Pathophysiology, Faculty of Medicine, "Victor Babeş" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timişoara, Romania; Center for Translational Research and Systems Medicine, "Victor Babeş" University of Medicine and Pharmacy, 2 Eftimie Murgu Sq., 300041 Timişoara, Romania.
authors.forename: Danina Mirela
authors.identifier: 0000-0001-6186-5321
authors.initials: DM
authors.lastname: Muntean
mesh_terms: D000818:Animals; D000890:Anti-Infective Agents / Q000737:chemistry / Q000494:pharmacology*; D000935:Antifungal Agents / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D001549:Benzamides / Q000737:chemistry / Q000494:pharmacology*; D016923:Cell Death / Q000187:drug effects; D045744:Cell Line, Tumor; D005658:Fungi / Q000187:drug effects; D006801:Humans; D008545:Melanoma / Q000473:pathology*; D051379:Mice; D008826:Microbial Sensitivity Tests; D014315:Triterpenes / Q000737:chemistry / Q000494:pharmacology*; D009828:Oleanolic Acid / Q000031:analogs & derivatives
chemical_list: D000890:Anti-Infective Agents; D000935:Antifungal Agents; D001549:Benzamides; D014315:Triterpenes; C412811:maslinic acid; D009828:Oleanolic Acid