title: Role of Interleukin-17A on the Chemotactic Responses to CCL7 in a Murine Allergic Rhinitis Model.
abstract: BACKGROUND
  The proinflammatory cytokine interleukin (IL)-17A is associated with eosinophil infiltration into the nasal mucosa in a mouse model of ovalbumin-induced allergic rhinitis. Chemotaxis of eosinophils is mediated primarily through C-C chemokine receptor type 3 (CCR3). However, the mechanism underlying the IL-17A-mediated enhancement of eosinophil recruitment via chemoattractants/chemokines remains unknown.
  
  
  OBJECTIVE
  In this study, we assessed the contribution of IL-17A to eosinophil-related inflammation via the CCL7/CCR3 pathway in experimental allergic rhinitis.
  
  
  METHODS
  IL-17A knockout (KO) and wild-type (WT) BALB/c mice were injected intraperitoneally and challenged intranasally with OVA to induce allergic rhinitis. Various parameters of the allergic response were evaluated, and mRNA and protein levels of CCL7 and CCR3 in nasal tissue and serum were compared between the two groups. The chemotactic response to CCL7 with or without IL-17A in bone marrow-derived eosinophils (bmEos) from BALB/c mice was measured.
  
  
  RESULTS
  In the allergic rhinitis model, IL-17A deficiency significantly decreased nasal symptoms, serum IgE levels, and eosinophil recruitment to the nasal mucosa. CCL7 and CCR3 mRNA and protein levels were decreased in the nasal mucosa of IL-17A KO mice compared with the WT mice. BmEos showed a significantly increased chemotactic response to -low concentration of CCL7 in the presence of IL-17A compared with its absence.
  
  
  CONCLUSIONS
  The suppression of nasal inflammation due of IL-17A deficiency in allergic rhinitis is partly responsible for the regulation of CCL7 secretion and eosinophil infiltration, which may be regulated via the CCL7/CCR3 pathway.
authors.affiliation: Department of Otorhinolaryngology-head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea.|Research Center for Sensory Organs, and Institute of Allergy, Seoul, Korea.
authors.forename: Yu-Lian
authors.initials: YL
authors.lastname: Zhang
authors.affiliation: Department of Otorhinolaryngology-head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Doo Hee
authors.initials: DH
authors.lastname: Han
authors.affiliation: Department of Otorhinolaryngology-head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea.
authors.forename: Dong-Young
authors.initials: DY
authors.lastname: Kim
authors.affiliation: Department of Otorhinolaryngology-head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea.|Research Center for Sensory Organs, and Institute of Allergy, Seoul, Korea.|Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggido.
authors.forename: Chul Hee
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Otorhinolaryngology-head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea.|Research Center for Sensory Organs, and Institute of Allergy, Seoul, Korea.|Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Bundang Hospital, Seongnam, Gyeonggido.|Clinical Immunology, Seoul National University Medical Research Center, Seoul, Korea.
authors.forename: Chae-Seo
authors.initials: CS
authors.lastname: Rhee
mesh_terms: D000818:Animals; D001854:Bone Marrow Cells / Q000473:pathology; D054410:Chemokine CCL7 / Q000235:genetics / Q000378:metabolism*; D002633:Chemotaxis; D004195:Disease Models, Animal; D004804:Eosinophils / Q000473:pathology; D005260:Female; D007073:Immunoglobulin E / Q000097:blood; D020381:Interleukin-17 / Q000378:metabolism*; D008807:Mice, Inbred BALB C; D018345:Mice, Knockout; D009297:Nasal Mucosa / Q000473:pathology; D010047:Ovalbumin / Q000276:immunology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D054397:Receptors, CCR3 / Q000378:metabolism; D065631:Rhinitis, Allergic / Q000097:blood / Q000378:metabolism* / Q000473:pathology*
chemical_list: C515451:Ccl7 protein, mouse; C515351:Ccr3 protein, mouse; D054410:Chemokine CCL7; D020381:Interleukin-17; D012333:RNA, Messenger; D054397:Receptors, CCR3; D007073:Immunoglobulin E; D010047:Ovalbumin