title: Using a redox-sensitive phosphorescent probe for optical evaluation of an intracellular redox environment.
abstract: A reducing intracellular environment is necessary for living cells. Here a redox-sensitive phosphorescent probe Ir-NO has been developed for evaluating the redox environment in living cells. Upon addition of reducing molecules, such as glutathione and ascorbic acid, the phosphorescent intensity of the probe is turned on, and the emission lifetime is elongated evidently. Furthermore, this probe has been used for optical imaging of the intracellular reducing environment by utilizing confocal laser scanning microscopy and phosphorescence lifetime imaging microscopy.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Liu
authors.forename: Na
authors.initials: N
authors.lastname: Zhou
authors.forename: Zejing
authors.initials: Z
authors.lastname: Chen
authors.forename: Huanjie
authors.initials: H
authors.lastname: Wei
authors.forename: Yana
authors.initials: Y
authors.lastname: Zhu
authors.forename: Song
authors.initials: S
authors.lastname: Guo
authors.forename: Qiang
authors.initials: Q
authors.lastname: Zhao
mesh_terms: D002468:Cell Physiological Phenomena; D018613:Microscopy, Confocal / Q000379:methods*; D061848:Optical Imaging / Q000379:methods*; D010084:Oxidation-Reduction