title: Using Electrophoretic Immunoassay to Monitor Hormone Secretion.
abstract: It has been demonstrated that microfluidic systems allow integration of sampling, reagent mixing, and rapid electrophoretic analysis. They have also proven useful for culturing cells wherein control over the environment allows novel and automated experiments. Here, we describe a microchip-based electrophoresis assay that allows cell culture and hormone monitoring. An online gradient generator can control cell culture condition precisely. This system has been applied for Pancreas islets' glucose sensitivity studies.
authors.affiliation: Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109, USA.
authors.forename: Shusheng
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109, USA. rtkenn@umich.edu.|Department of Pharmacology, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI, 48109, USA. rtkenn@umich.edu.
authors.forename: Robert T
authors.initials: RT
authors.lastname: Kennedy
mesh_terms: D000818:Animals; D019075:Electrophoresis, Capillary / Q000295:instrumentation / Q000379:methods*; D046209:Electrophoresis, Microchip / Q000295:instrumentation / Q000379:methods; D004867:Equipment Design; D005947:Glucose; D006728:Hormones / Q000097:blood / Q000378:metabolism; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D007328:Insulin / Q000378:metabolism; D000078790:Insulin Secretion; D007515:Islets of Langerhans / Q000378:metabolism; D051379:Mice; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods
chemical_list: D006728:Hormones; D007328:Insulin; D005947:Glucose
keywords: Capillary electrophoresis; Competitive immunoassay; Diabetes; Glucose sensitivity; Islets of Langerhans; Microfluidics; Perfusion culture