title: MicroRNA-133b Inhibits Cell Proliferation and Invasion in Osteosarcoma by Targeting Sirt1.
abstract: MicroRNAs are a class of small noncoding RNAs that function as critical gene regulators through targeting mRNAs for translational repression or degradation. In this study, we showed that the miR-133b expression level was decreased while the Sirt1 mRNA expression level was increased in osteosarcoma tissue and cell lines. A low expression of miR-133b was significantly associated with tumor size, distant metastasis, and advanced clinical stage. In addition, osteosarcoma patients with a low miR-133b expression showed a worse prognosis when compared to those with a high level of miR-133b expression. Thus, we identified Sirt1 as a novel direct target of miR-133b. Overexpression of miR-133b suppressed Sirt1 expression and attenuated cell proliferation and invasion. Forced expression of Sirt1 could partly rescue the inhibitory effect of miR-133b in osteosarcoma cells. Our finding also suggested that the inhibitory effects of the miR-133b/Sirt1 axis on osteosarcoma progression were involved in the Wnt/β-catenin pathway. Taken together, these findings will shed light on the role and mechanism of miR-133b in regulating osteosarcoma cell growth via the miR-133b/Sirt1 axis, and miR-133b may serve as a potential therapeutic target in osteosarcoma in the future.
authors.forename: Shi
authors.initials: S
authors.lastname: Ying
authors.forename: Huang
authors.initials: H
authors.lastname: Jianjun
authors.forename: Yi
authors.initials: Y
authors.lastname: Xue
authors.forename: Yu
authors.initials: Y
authors.lastname: Shuwei
authors.forename: Zhang
authors.initials: Z
authors.lastname: Liyuan
authors.forename: Wang
authors.initials: W
authors.lastname: Jie
authors.forename: Cheng
authors.initials: C
authors.lastname: Lixian
mesh_terms: D001859:Bone Neoplasms / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D018450:Disease Progression; D015536:Down-Regulation; D057809:HEK293 Cells; D006801:Humans; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics* / Q000378:metabolism; D009361:Neoplasm Invasiveness; D012516:Osteosarcoma / Q000235:genetics* / Q000378:metabolism / Q000473:pathology; D012333:RNA, Messenger / Q000096:biosynthesis / Q000235:genetics; D056564:Sirtuin 1 / Q000235:genetics* / Q000378:metabolism; D014162:Transfection; D060449:Wnt Signaling Pathway
chemical_list: C548234:MIRN133 microRNA, human; D035683:MicroRNAs; D012333:RNA, Messenger; C447939:SIRT1 protein, human; D056564:Sirtuin 1