title: Novel blood-based microRNA biomarker panel for early diagnosis of chronic pancreatitis.
abstract: Chronic pancreatitis (CP) is an inflammatory disease characterized by progressive fibrosis of pancreas. Early diagnosis will improve the prognosis of patients. This study aimed to obtain serum miRNA biomarkers for early diagnosis of CP. In the current study, we analyzed the differentially expressed miRNAs (DEmiRs) of CP patients from Gene Expression Omnibus (GEO), and the DEmiRs in plasma of early CP patients (n = 10) from clinic by miRNA microarrays. Expression levels of DEmiRs were further tested in clinical samples including early CP patients (n = 20), late CP patients (n = 20) and healthy controls (n = 18). The primary endpoints were area under curve (AUC) and expression levels of DEmiRs. Four DEmiRs (hsa-miR-320a-d) were obtained from GEO CP, meanwhile two (hsa-miR-221 and hsa-miR-130a) were identified as distinct biomarkers of early CP by miRNA microarrays. When applied on clinical serum samples, hsa-miR-320a-d were accurate in predicting late CP, while hsa-miR-221 and hsa-miR-130a were accurate in predicting early CP with AUC of 100.0% and 87.5%. Our study indicates that miRNA expression profile is different in early and late CP. Hsa-miR-221 and hsa-miR-130a are biomarkers of early CP, and the panel of the above 6 serum miRNAs has the potential to be applied clinically for early diagnosis of CP.
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Xin
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Jin-Huan
authors.initials: JH
authors.lastname: Lin
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Zhuan
authors.initials: Z
authors.lastname: Liao
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Jun-Tao
authors.initials: JT
authors.lastname: Ji
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Ting-Ting
authors.initials: TT
authors.lastname: Du
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Fei
authors.initials: F
authors.lastname: Jiang
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Liang-Hao
authors.initials: LH
authors.lastname: Hu
authors.affiliation: Department of Gastroenterology, Changhai Hospital, the Second Military Medical University, Shanghai, China.
authors.forename: Zhao-Shen
authors.initials: ZS
authors.lastname: Li
mesh_terms: D000328:Adult; D019540:Area Under Curve; D015415:Biomarkers / Q000097:blood; D016022:Case-Control Studies; D016000:Cluster Analysis; D042241:Early Diagnosis; D005260:Female; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000097:blood* / Q000378:metabolism; D020411:Oligonucleotide Array Sequence Analysis; D050500:Pancreatitis, Chronic / Q000175:diagnosis*; D012333:RNA, Messenger / Q000378:metabolism; D012372:ROC Curve
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D012333:RNA, Messenger