title: [A study of immune response to HBsAg mediated by CD4+ memory T lymphocytes in immunized HBV transgenic mice].
abstract: Objective:To investigate the mechanism of immune response to HBsAg mediated by CD4+ memory T lymphocytes in immunized HBV transgenic (Tg) mice.Methods:Peripheral CD4+ memory T lymphocytes were isolated and collected by magnetic bead sorting from C57BL/6 mice successfully immunized by hepatitis B vaccine. Adoptive immunization was performed for Tg mice by caudal vein injection, and the peripheral blood was collected at 4 and 8 weeks after adoptive immunization. Enzyme-linked immunosorbent assay was used to measure the levels of HBsAg, anti-HBs, interferon-γ (IFNγ), and interleukin-2 (IL-2), and immunohistochemistry was used to measure the level of HBsAg in the liver. The t-test was used for comparison of continuous data, and the chi-square test was used for comparison of categorical data.Results:Compared with the control group, the experimental group had a significant reduction in HBsAg titer[(243.5±12.4) ng/mlvs0 ng/ml,t= 3.939,P= 0.011] and significant increases in the levels of anti-HBs [0 ng/ml vs (530.7±17.5) ng/ml,t= 4.414,P= 0.006 9], IFNγ [(12.25±2.9) pg/mlvs(38.8±6.8) pg/ml,t= 2.785,P= 0.049 6], and IL-2 [(11.3±3.5) pg/mlvs(82.3±9.2) pg/ml,t= 2.993,P= 0.040 2].Conclusion:As for immune response to HBsAg mediated by CD4+ memory T lymphocytes in immunized HBV Tg mice, increases in the levels of IFNγ and IL-2 help to enhance humoral and cellular immunity and mediate the clearance of HBsAg in Tg mice.
authors.forename: Y
authors.initials: Y
authors.lastname: Li
authors.forename: J H
authors.initials: JH
authors.lastname: Chen
authors.forename: X G
authors.initials: XG
authors.lastname: Shu
authors.forename: J H
authors.initials: JH
authors.lastname: Chen
authors.forename: Q H
authors.initials: QH
authors.lastname: Xia
authors.affiliation: Department of Gastrointestinal Minimal Invasive Surgery, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China.
authors.forename: W Z
authors.initials: WZ
authors.lastname: Yuan
authors.forename: L
authors.initials: L
authors.lastname: Wu
authors.forename: Q
authors.initials: Q
authors.lastname: Tang
authors.forename: G B
authors.initials: GB
authors.lastname: Wang
mesh_terms: D000818:Animals; D015496:CD4-Positive T-Lymphocytes; D004797:Enzyme-Linked Immunosorbent Assay; D006509:Hepatitis B; D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007111:Immunity, Cellular; D007114:Immunization; D007371:Interferon-gamma; D008099:Liver; D051379:Mice; D008810:Mice, Inbred C57BL; D008822:Mice, Transgenic
chemical_list: D006510:Hepatitis B Antibodies; D006514:Hepatitis B Surface Antigens; D017325:Hepatitis B Vaccines; D007371:Interferon-gamma