title: Environmental DNA Detection of the Golden Tree Frog (Phytotriades auratus) in Bromeliads.
abstract: The analysis of environmental DNA (eDNA) is a powerful, non-destructive technique for detecting rare or hard to find freshwater organisms. In this study, we investigated the effectiveness of environmental DNA analysis as a method for detecting a rare amphibian, the golden tree frog (Phytotriades auratus). These frogs are believed to live exclusively within one species of tank bromeliad, Glomeropitcairnia erectiflora, found on the highest peaks of the island of Trinidad in the West Indies. Previous survey methods for this species involved bromeliad destruction, while here we collected and analyzed water samples from discrete pools within G. erectiflora plants for species-specific DNA. We found 1) that we can identify the presence of P. auratus in the bromeliads using environmental DNA analysis, and 2) that environmental DNA evidence indicates the presence of a previously undiscovered P. auratus population, increasing the species' range from two isolated 'sky islands' to three.
authors.affiliation: Independent Scholar, Portland, Oregon, United States of America.
authors.forename: Jack D
authors.initials: JD
authors.lastname: Torresdal
authors.affiliation: Department of Life Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago.
authors.forename: Aidan D
authors.initials: AD
authors.lastname: Farrell
authors.affiliation: School of the Environment, Washington State University, Pullman, Washington, United States of America.
authors.forename: Caren S
authors.initials: CS
authors.lastname: Goldberg
mesh_terms: D000818:Animals; D004247:DNA / Q000032:analysis* / Q000235:genetics*; D016133:Polymerase Chain Reaction; D011898:Ranidae / Q000235:genetics*; D014300:Trinidad and Tobago; D014900:West Indies
chemical_list: D004247:DNA