title: Protective Effect of Cactus Cladode Extracts on Peroxisomal Functions in Microglial BV-2 Cells Activated by Different Lipopolysaccharides.
abstract: In this study, we aimed to evaluate the antioxidant and anti-inflammatory properties ofOpuntia ficus-indicacactus cladode extracts in microglia BV-2 cells. Inflammation associated with microglia activation in neuronal injury can be achieved by LPS exposure. Using four different structurally and biologically well-characterized LPS serotypes, we revealed a structure-related differential effect of LPS on fatty acid β-oxidation and antioxidant enzymes in peroxisomes:Escherichia coli-LPS decreased ACOX1 activity whileSalmonella minnesota-LPS reduced only catalase activity. Different cactus cladode extracts showed an antioxidant effect through microglial catalase activity activation and an anti-inflammatory effect by reducing nitric oxide (NO) LPS-dependent production. These results suggest that cactus extracts may possess a neuroprotective activity through the induction of peroxisomal antioxidant activity and the inhibition of NO production by activated microglial cells.
authors.affiliation: Laboratoire BioPeroxIL, Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique, EA 7270, Unité de Formation et de Recherche des Sciences Vie, Terre et Environnement, 21000 Dijon, France. f.z.saih@hotmail.com.|Laboratoire BioPeroxIL, Université Bourgogne-Franche Comté, 6 Bd Gabriel, 21000 Dijon, France. f.z.saih@hotmail.com.|Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan I, BP 577, 26 000 Settat, Morocco. f.z.saih@hotmail.com.
authors.forename: Fatima-Ezzahra
authors.initials: FE
authors.lastname: Saih
authors.affiliation: Laboratoire BioPeroxIL, Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique, EA 7270, Unité de Formation et de Recherche des Sciences Vie, Terre et Environnement, 21000 Dijon, France. pierre.andreoletti@u-bourgogne.fr.|Laboratoire BioPeroxIL, Université Bourgogne-Franche Comté, 6 Bd Gabriel, 21000 Dijon, France. pierre.andreoletti@u-bourgogne.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Andreoletti
authors.affiliation: Lipness Team, INSERM, Research Center UMR866 and LabEx LipSTIC, Faculté de Médecine, Université de Bourgogne-Franche Comté, 21079 Dijon CEDEX, France. stephane.mandard@u-bourgogne.fr.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Mandard
authors.affiliation: Laboratoire BioPeroxIL, Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique, EA 7270, Unité de Formation et de Recherche des Sciences Vie, Terre et Environnement, 21000 Dijon, France. norbert.latruffe@u-bourgogne.fr.|Laboratoire BioPeroxIL, Université Bourgogne-Franche Comté, 6 Bd Gabriel, 21000 Dijon, France. norbert.latruffe@u-bourgogne.fr.
authors.forename: Norbert
authors.initials: N
authors.lastname: Latruffe
authors.affiliation: Laboratoire Biologie et Santé, Faculté des Sciences Ben M'sik, Université Hassan II-Casablanca, Avenue Cdt Driss El Harti BP 7955, 20100 Casablanca, Morocco. mselkebbaj@yahoo.fr.
authors.forename: M'Hammed Saïd
authors.initials: MS
authors.lastname: El Kebbaj
authors.affiliation: Laboratoire BioPeroxIL, Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique, EA 7270, Unité de Formation et de Recherche des Sciences Vie, Terre et Environnement, 21000 Dijon, France. gerard.lizard@u-bourgogne.fr.|Laboratoire BioPeroxIL, Université Bourgogne-Franche Comté, 6 Bd Gabriel, 21000 Dijon, France. gerard.lizard@u-bourgogne.fr.
authors.forename: Gérard
authors.initials: G
authors.lastname: Lizard
authors.affiliation: Laboratoire de Biochimie et Neurosciences, Faculté des Sciences et Techniques, Université Hassan I, BP 577, 26 000 Settat, Morocco. boubker_nasser@hotmail.com.
authors.forename: Boubker
authors.initials: B
authors.lastname: Nasser
authors.affiliation: Laboratoire BioPeroxIL, Biochimie du Peroxysome, Inflammation et Métabolisme Lipidique, EA 7270, Unité de Formation et de Recherche des Sciences Vie, Terre et Environnement, 21000 Dijon, France. malki@u-bourgogne.fr.|Laboratoire BioPeroxIL, Université Bourgogne-Franche Comté, 6 Bd Gabriel, 21000 Dijon, France. malki@u-bourgogne.fr.
authors.forename: Mustapha
authors.initials: M
authors.lastname: Cherkaoui-Malki
mesh_terms: D000818:Animals; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000975:Antioxidants / Q000494:pharmacology*; D002374:Catalase / Q000378:metabolism*; D002460:Cell Line; D004926:Escherichia coli; D005227:Fatty Acids / Q000378:metabolism; D008070:Lipopolysaccharides; D051379:Mice; D017628:Microglia / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D018696:Neuroprotective Agents / Q000494:pharmacology; D009569:Nitric Oxide / Q000378:metabolism*; D029747:Opuntia / Q000737:chemistry*; D010084:Oxidation-Reduction / Q000187:drug effects; D018384:Oxidative Stress / Q000187:drug effects; D020675:Peroxisomes / Q000378:metabolism*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species / Q000378:metabolism; D012475:Salmonella
chemical_list: D000893:Anti-Inflammatory Agents; D000975:Antioxidants; D005227:Fatty Acids; D008070:Lipopolysaccharides; D018696:Neuroprotective Agents; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D009569:Nitric Oxide; D002374:Catalase
keywords: Escherichia coli; LPS; Opuntia; Salmonella minnesota; acyl-CoA oxidase 1; catalase; lipopolysaccharides; nitric oxide; peroxisomes; β-oxidation