title: Evaluating the probiotic and therapeutic potentials of Saccharomyces cerevisiae strain (OBS2) isolated from fermented nectar of toddy palm.
abstract: The purpose of this study is to evaluate the probiotic characteristics of 15 yeast strains isolated from nectar of toddy palm. Initially, the collected samples were inoculated on yeast extract peptone dextrose agar plates and the colonies so obtained were culturally and morphologically characterized. Commercial probiotic yeast, Saccharomyces boulardii served as the control in these experiments. Of the 15 yeast strains, the isolates that were resistant to antibiotics and worked synergistically with other cultures were considered for further evaluation. Selected isolates were evaluated in vitro for tolerance to simulated gastrointestinal conditions such as temperature, pH, bile and gastric juice. Further the yeast isolates were evaluated for their pathogenicity and adherence to intestinal epithelial cells. The 2 yeast isolates with efficient probiotic properties were finally characterized by sequencing their 5.8 S rRNA and partial sequences of internal transcribed spacer 1 and 2. The sequences were BLAST searched in the National Center for Biotechnology Information, nucleic acid database for sequence similarity of organisms and phylogenetic evolutionary analysis was carried out. Based on maximum similarity of basic local alignment search tool results, organisms were characterized as Pichia kudriavzevii OBS1 (100%) and Saccharomyces cerevisiae OBS2 (96%) and sequences were finally deposited in the GenBank data library. Among these two isolates, S. cerevisiae OBS2 displayed slight/moderate antioxidant and anticancer property. Hence, strain OBS2 can be utilized and explored as a potential probiotic for therapeutic applications.
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India.
authors.forename: Banoth
authors.initials: B
authors.lastname: Srinivas
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India.
authors.forename: Ganapathiwar Swarupa
authors.initials: GS
authors.lastname: Rani
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India.
authors.forename: Bhukya Kiran
authors.initials: BK
authors.lastname: Kumar
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India.
authors.forename: Banoth
authors.initials: B
authors.lastname: Chandrasekhar
authors.affiliation: Center for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Telangana, 500007, India.
authors.forename: Kommalapati Vamsi
authors.initials: KV
authors.lastname: Krishna
authors.affiliation: Center for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Tarnaka, Hyderabad, Telangana, 500007, India.
authors.forename: Tangutur Anjana
authors.initials: TA
authors.lastname: Devi
authors.affiliation: Department of Microbiology, University College of Science, Osmania University, Hyderabad, Telangana, 500007, India. bhima.ou@gmail.com.
authors.forename: Bhukya
authors.identifier: http://orcid.org/0000-0002-1343-3863
authors.initials: B
authors.lastname: Bhima
keywords: Antimicrobial resistance; Probiotics; Therapeutics; Toddy nectar; Yeasts