title: Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars.
abstract: Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
authors.forename: Toshihiro
authors.initials: T
authors.lastname: Kasama
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.
authors.forename: Noritada
authors.initials: N
authors.lastname: Kaji
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.|Division of Biotechnology and Macromolecular Chemistry, Hokkaido University, Sapporo, Japan.
authors.forename: Manabu
authors.initials: M
authors.lastname: Tokeshi
authors.affiliation: Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. babaymtt@apchem.nagoya-u.ac.jp.|Division of Biotechnology and Macromolecular Chemistry, Hokkaido University, Sapporo, Japan. babaymtt@apchem.nagoya-u.ac.jp.|Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. babaymtt@apchem.nagoya-u.ac.jp.|Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, 761-0395, Japan. babaymtt@apchem.nagoya-u.ac.jp.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, Japan. babaymtt@apchem.nagoya-u.ac.jp.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D000906:Antibodies; D004867:Equipment Design; D005455:Fluorescent Antibody Technique; D020100:Hydrogels / Q000737:chemistry; D007118:Immunoassay / Q000295:instrumentation* / Q000379:methods; D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008863:Microspheres; D016062:Porosity
chemical_list: D000906:Antibodies; D020100:Hydrogels
keywords: Biomarker; Lab-on-a-chip; Microfluidic immunoassay