title: Pelagostrobilidium liui n. sp. (Ciliophora, Choreotrichida) from the Coastal Waters of Northeastern Taiwan and an Improved Description of Pelagostrobilidium minutum Liu et al., 2012.
abstract: The number of somatic kineties in Pelagostrobilidium ranges from 4 to 6 according to the present state of knowledge. This study investigates Pelagostrobilidium liui n. sp. using live observation, protargol stain, and small subunit rDNA data sequencing. Pelagostrobilidium liui n. sp. is characterized by having a spherical-shaped body, four somatic kineties, with kinety 2 spiraled around the left side of body, about six elongated external membranelles, and invariably no buccal membranelle. It differs from its most similar congener, Pelagostrobilidium minutum Liu et al., , in (i) cell shape; (ii) macronucleus width; (iii) oral apparatus; (iv) anterior orientation of kinety 2; (v) location where kinety 2 commences; (vi) arrangement of kinety 1; (vii) distance between the anterior cell end and the locations where kineties commence; and (viii) the presence of 12 different bases (including two deletions) in the small subunit rDNA sequences. The diagnosis of P. minutum Liu et al., is also improved to include the following new characteristics: invariably four somatic kineties; kineties 2 and 4 alone commence at the same level; kinety 2 originates from right anterior cell half on ventral side, extends sinistrally posteriorly, over kinety 1, around left posterior region, terminates near posterior cell end on dorsal side; kinety 1 commences below anterior third of kinety 2.
authors.affiliation: Institute of Environmental Biology and Fisheries Science, National Taiwan Ocean University, Keelung, 202-24, Taiwan.
authors.forename: Po-Cheng
authors.initials: PC
authors.lastname: Chen
authors.affiliation: Institute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, 202-24, Taiwan.|Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, 202-24, Taiwan.
authors.forename: Kuo-Ping
authors.initials: KP
authors.lastname: Chiang
authors.affiliation: Institute of Marine Environment and Ecology, National Taiwan Ocean University, Keelung, 202-24, Taiwan.|Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, 202-24, Taiwan.
authors.forename: Sheng-Fang
authors.initials: SF
authors.lastname: Tsai
mesh_terms: D016798:Ciliophora / Q000145:classification* / Q000302:isolation & purification / Q000648:ultrastructure*; D004275:DNA, Ribosomal / Q000235:genetics; D010802:Phylogeny; D010933:Plankton / Q000145:classification / Q000302:isolation & purification / Q000648:ultrastructure; D017422:Sequence Analysis, DNA / Q000379:methods*; D013624:Taiwan
chemical_list: D004275:DNA, Ribosomal
keywords: Ciliary pattern; Strobilidiidae; molecular phylogeny; morphology; protozoa