title: Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer.
abstract: Based on our previous studies, differential analysis of N-glycan expression bound on serum haptoglobin reveals the quantitative variation on gastric cancer patients. In this prospective case-control study, we explore the clinically relevant glycan markers for gastric cancer diagnosis. Serum samples were collected from patients with gastric cancer (n = 44) and healthy control (n = 44). N-glycans alteration was monitored by intact analysis of Hp using liquid chromatography-mass spectrometry followed by immunoaffinity purification with the serum samples. Intensity and frequency markers were defined depending on the mass spectrometry data analysis. Multiple markers were found with high diagnostic efficacy. As intensity markers (I-marker), six markers were discovered with the AUC > 0.8. The high efficiency markers exhibited AUC of 0.93 with a specificity of 86% when the sensitivity was set to 95%. We additionally established frequency marker (f-marker) panels based on the tendency of high N-glycan expression. The AUC to conclude patients and control group were 0.82 and 0.79, respectively. This study suggested that N-glycan variation of serum haptoglobin were associated with patients with gastric cancer and might be a promising marker for the cancer screening.
authors.affiliation: Department of Food and Nutrition, Chungnam National University, Yuseong-Gu, Deajeon, Korea.
authors.forename: Jae-Han
authors.initials: JH
authors.lastname: Kim
authors.affiliation: GLYCAN Co., Ltd., Healthcare Innovation Park, Bundang-Gu, Seongnam, Korea.
authors.forename: Sung Hyeon
authors.initials: SH
authors.lastname: Lee
authors.affiliation: GLYCAN Co., Ltd., Healthcare Innovation Park, Bundang-Gu, Seongnam, Korea.
authors.forename: Sookyung
authors.initials: S
authors.lastname: Choi
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-Gu, Deajeon, Korea.
authors.forename: Unyong
authors.initials: U
authors.lastname: Kim
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-Gu, Daejeon, Korea.
authors.forename: In Seok
authors.initials: IS
authors.lastname: Yeo
authors.affiliation: GLYCAN Co., Ltd., Healthcare Innovation Park, Bundang-Gu, Seongnam, Korea.
authors.forename: Su Hee
authors.initials: SH
authors.lastname: Kim
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-Gu, Deajeon, Korea.
authors.forename: Myung Jin
authors.initials: MJ
authors.lastname: Oh
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-Gu, Deajeon, Korea.
authors.forename: Hantae
authors.initials: H
authors.lastname: Moon
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-Gu, Deajeon, Korea.
authors.forename: Jua
authors.initials: J
authors.lastname: Lee
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-Gu, Deajeon, Korea.
authors.forename: Seunghyup
authors.initials: S
authors.lastname: Jeong
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Min Gew
authors.initials: MG
authors.lastname: Choi
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Jun Ho
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Tae Sung
authors.initials: TS
authors.lastname: Sohn
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Jae Moon
authors.initials: JM
authors.lastname: Bae
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Sung
authors.initials: S
authors.lastname: Kim
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Yang Won
authors.initials: YW
authors.lastname: Min
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Hyuk
authors.initials: H
authors.lastname: Lee
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Jun Haeng
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Poong-Lyul
authors.initials: PL
authors.lastname: Rhee
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Jae J
authors.initials: JJ
authors.lastname: Kim
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Su Jin
authors.initials: SJ
authors.lastname: Lee
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Seung Tae
authors.initials: ST
authors.lastname: Kim
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Jeeyun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Se Hoon
authors.initials: SH
authors.lastname: Park
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Joon Oh
authors.initials: JO
authors.lastname: Park
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Young Suk
authors.initials: YS
authors.lastname: Park
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Ho Yeong
authors.initials: HY
authors.lastname: Lim
authors.affiliation: Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea.
authors.forename: Won Ki
authors.initials: WK
authors.lastname: Kang
authors.affiliation: Graduate School of Analytical Science and Technology, Chungnam National University, Yuseong-Gu, Deajeon, Korea.
authors.forename: Hyun Joo
authors.initials: HJ
authors.lastname: An
authors.affiliation: Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Yuseong-Gu, Daejeon, Korea.
authors.forename: Jung Hoe
authors.initials: JH
authors.lastname: Kim
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000097:blood / Q000378:metabolism*; D016022:Case-Control Studies; D002853:Chromatography, Liquid; D055088:Early Detection of Cancer / Q000379:methods; D005260:Female; D006031:Glycosylation; D006242:Haptoglobins / Q000378:metabolism*; D006801:Humans; D008297:Male; D013058:Mass Spectrometry; D008875:Middle Aged; D011134:Polysaccharides / Q000378:metabolism*; D011446:Prospective Studies; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013274:Stomach Neoplasms / Q000097:blood / Q000175:diagnosis / Q000378:metabolism*
chemical_list: D014408:Biomarkers, Tumor; D006242:Haptoglobins; D011134:Polysaccharides
keywords: N-glycan; biomarker; glycoprotein; haptoglobin; stomach neoplasm