title: Discovering potential serological biomarker for chronic Hepatitis B Virus-related hepatocellular carcinoma in Chinese population by MAL-associated serum glycoproteomics analysis.
abstract: The accuracy of current biomarkers for the diagnosis of hepatocellular carcinoma (HCC), especially chronic Hepatitis B Virus (HBV)-related HCC, is limited. Recent progress in glycoproteomics has provided a novel platform for screening novel serological biomarkers of HCC. In this study, lectin affinity chromatography by Maackia amurensis lectin (MAL) and iTRAQ combined with mass spectrometric analysis were performed to enrich and identify the glycoprotein fractions in serum samples from HBV-related HCC patients and from healthy controls. Seventeen differential MAL-associated glycoproteins were identified. Among them, Galectin 3 binding protein (Gal-3BP) was selected for further evaluated by ELISA analysis and showed a high diagnostic potential of HBV-related HCC, with the AUC of 0.898 and a sensitivity, specificity and accuracy of 80.00%, 93.75% and 86.88%, respectively. Moreover, we constructed a predictive model through the combined use of serum Gal-3BP and Alpha Fetoprotein (AFP), which improved the sensitivity (from 87.5% to 95%), specificity (from 93.75% to 95%) and accuracy (from 90.63% to 95%) of diagnosing early HCC. These data suggested serum Gal-3BP level is a promising biomarker to identify HBV-related HCC and the combined use of serum Gal-3BP and AFP improves the diagnostic potential of HBV-HCC compared with AFP alone in current clinical practice.
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Tianhua
authors.initials: T
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, China.
authors.forename: Denghe
authors.initials: D
authors.lastname: Liu
authors.affiliation: People's Hospital of Gangxi Zhuang Autonomous Region, Nanning, Guangxi, China.
authors.forename: Riqiang
authors.initials: R
authors.lastname: Liu
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Hucong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Yan
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Sun
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Shu
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Yinkun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Liver Cancer Institute, Zhongshan Hospital, Fudan University, Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, Cancer Research Center, Institutes of Biomedical Sciences, Fudan University, Shanghai, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Guo
mesh_terms: D000951:Antigens, Neoplasm / Q000097:blood*; D044466:Asian People; D015415:Biomarkers / Q000097:blood*; D014408:Biomarkers, Tumor / Q000097:blood*; D006528:Carcinoma, Hepatocellular / Q000175:diagnosis* / Q000473:pathology; D002352:Carrier Proteins / Q000097:blood*; D002853:Chromatography, Liquid; D004797:Enzyme-Linked Immunosorbent Assay; D006023:Glycoproteins / Q000097:blood*; D019694:Hepatitis B, Chronic / Q000150:complications*; D006801:Humans; D037102:Lectins / Q000378:metabolism; D008113:Liver Neoplasms / Q000175:diagnosis* / Q000473:pathology; D029901:Maackia / Q000737:chemistry; D013058:Mass Spectrometry; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D000509:alpha-Fetoproteins / Q000032:analysis*; D016326:Extracellular Matrix Proteins
chemical_list: D000951:Antigens, Neoplasm; D015415:Biomarkers; D014408:Biomarkers, Tumor; D002352:Carrier Proteins; D006023:Glycoproteins; D037102:Lectins; D000509:alpha-Fetoproteins; C081856:LGALS3BP protein, human; D016326:Extracellular Matrix Proteins