title: Alterations in transcription and protein expressions of HCC-related genes in HepG2 cells caused by microcystin-LR.
abstract: Microcystin-LR (MC-LR) is the most common and toxic hepatotoxin and it could induce human hepatitis and hepatocellular carcinoma (HCC) via the route of drinking water. The aim of the present study was to determine the expressions of oncogenes c-fos, c-jun, c-myc, c-met, and N-ras and tumor suppressor gene PTEN in HepG2 cells following MC-LR-exposure to understand the possible mechanism of MC-LR-related human primary liver cancer. The results of qPCR and Western blotting showed that MC-LR-exposure at non- or sub-cytotoxic concentrations promoted the expressions of oncogenes c-fos, c-jun, c-myc, c-met, and N-ras while suppressed tumor-suppressor gene PTEN in HepG2 cells at both transcription and protein levels. This result suggests that HCC-related genes may be involved in human hepatitis and primary liver cancer caused by MC-LR. The work might be useful for evaluating the human health risk resulted from the long-term of MC-LR-exposure at low dose via drinking water route.
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China. Electronic address: lixiaoyu65@263.net.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors.forename: Xiangyang
authors.initials: X
authors.lastname: Zhang
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Xie
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors.forename: Chune
authors.initials: C
authors.lastname: Zhou
authors.affiliation: College of Life Science, Henan Normal University, Xinxiang, Henan 453007, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Life Sciences and Technology, Xinxiang Medical University, Henan 453003, China.
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics* / Q000378:metabolism; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics* / Q000378:metabolism; D008387:Marine Toxins; D052998:Microcystins / Q000633:toxicity*; D015513:Oncogene Proteins / Q000235:genetics / Q000378:metabolism; D051059:PTEN Phosphohydrolase / Q000235:genetics / Q000378:metabolism; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D008387:Marine Toxins; D052998:Microcystins; D015513:Oncogene Proteins; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human; C057862:cyanoginosin LR
keywords: Expression; HepG2; Microcystin-LR; Oncogene; Tumor-suppressor gene