title: Polyurethane conjugating TGF-β on surface impacts local inflammation and endoplasmic reticulum stress in skeletal muscle.
abstract: The synthesized short peptide-polymers would provide key functions for tissue regeneration and repair, through enriching bioactive molecules on polymers or releasing these molecules pre-conjugated on the materials. We have developed a degradable polyurethane (PU) bearing HSNGLPL peptide, which has affinity binding ability to transforming growth factor-betas (TGF-β). For deeply understanding spatial release of TGF-β from the PU polymers and its localized bioactivity, quartz crystal microbalance (QCM) and Elisa test were used to verify TGF-β binding capacities in vitro and in vivo. The PU polymers, with or without pre-conjugating of TGF-β, were implanted into gastronomies muscle (GN) of C57BL/6 mice, for addressing TGF-β release from the polymers and its bio-regulating function in vivo. QCM result shows that PU bearing HSNGLPL peptide has affinity binding ability to TGF-β in vitro. Intramuscular implanting experiment further supports the enrichment efficiency of TGF-β on PU polymers in vivo. The detecting data involving intramuscular inflammatory infiltration triggered by the implants, myofiber regeneration, muscular fibrosis degree, and activation of endoplasmic reticulum stress (ER stress), evidence TGF-β can be released from PU polymers, and exerts regulating effects on the material-induced inflammation. Thus, our present results suggest it is feasible to improve biocompatibility of PU polymers in vivo, by pre-bearing bioactive molecules on materials before the implanting. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1156-1165, 2017.
authors.affiliation: Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, GuangZhou, 510515, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Shi
authors.affiliation: Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, GuangZhou, 510515, China.
authors.forename: Jiangwei
authors.initials: J
authors.lastname: Xiao
authors.affiliation: Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, GuangZhou, 510515, China.
authors.forename: Ruicai
authors.initials: R
authors.lastname: Gu
authors.affiliation: School of Materials Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
authors.forename: Gang
authors.identifier: 0000-0002-4730-4971
authors.initials: G
authors.lastname: Wu
authors.affiliation: Department of Anatomy, Guangdong Provincial Key Laboratory of Construction and Detection in Tissue Engineering, Southern Medical University, GuangZhou, 510515, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Liao
mesh_terms: D000818:Animals; D003692:Delayed-Action Preparations / Q000737:chemistry / Q000494:pharmacology; D059865:Endoplasmic Reticulum Stress / Q000187:drug effects*; D020007:Implants, Experimental; D007249:Inflammation / Q000378:metabolism / Q000473:pathology / Q000517:prevention & control; D051379:Mice; D018482:Muscle, Skeletal / Q000378:metabolism* / Q000473:pathology; D010455:Peptides / Q000737:chemistry; D011140:Polyurethanes / Q000737:chemistry*; D016212:Transforming Growth Factor beta / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D003692:Delayed-Action Preparations; D010455:Peptides; D011140:Polyurethanes; D016212:Transforming Growth Factor beta
keywords: ER stress; HSNGLPL peptide; TGF-β; inflammation; polyurethanes