title: Comparative study of the binding of 3 flavonoids to the fat mass and obesity-associated protein by spectroscopy and molecular modeling.
abstract: This study aims to investigate the interaction between 3 flavonoids (quercetin, apigenin, and naringenin) and fat mass and obesity-associated protein by fluorescence, ultraviolet-visible absorption spectroscopy, and molecular modeling. Results indicate that the intrinsic fluorescence of fat mass and obesity-associated protein can be quenched by the 3 flavonoids through a static quenching procedure. Thermodynamic analysis and molecular modeling results suggest that hydrophobic interaction and hydrogen bond forces play the major roles in the binding process. Moreover, results also show that the rank order of quenching constant and binding constant is quercetin > apigenin > naringenin.
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Lijiao
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Ren
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Zechun
authors.initials: Z
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Ruiyong
authors.initials: R
authors.lastname: Wang
authors.affiliation: College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou, China.
authors.forename: Junbiao
authors.initials: J
authors.lastname: Chang
mesh_terms: D000273:Adipose Tissue / Q000737:chemistry* / Q000378:metabolism; D047310:Apigenin / Q000737:chemistry / Q000494:pharmacology; D001665:Binding Sites; D044950:Flavanones / Q000737:chemistry / Q000494:pharmacology; D005419:Flavonoids / Q000737:chemistry / Q000494:pharmacology*; D006860:Hydrogen Bonding; D057927:Hydrophobic and Hydrophilic Interactions; D020738:Leptin / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D062105:Molecular Docking Simulation / Q000379:methods*; D011485:Protein Binding; D011794:Quercetin / Q000737:chemistry / Q000494:pharmacology; D013050:Spectrometry, Fluorescence; D013816:Thermodynamics
chemical_list: D044950:Flavanones; D005419:Flavonoids; D020738:Leptin; D047310:Apigenin; D011794:Quercetin; C005273:naringenin
keywords: FTO; flavonoids; fluorescence; interaction; molecular modeling