title: Transcriptome and Gene Expression Analysis of Cylas formicarius (Coleoptera: Brentidae) During Different Development Stages.
abstract: The sweet potato weevil, Cylas formicarius (F.) (Coleoptera: Brentidae), is an important pest of sweet potato worldwide. However, there is limited knowledge on the molecular mechanisms underlying growth and differentiation of C. formicarius The transcriptomes of the eggs, second instar larvae, third instar larvae (L3), pupae, females, and males of C. formicarius were sequenced using Illumina sequencing technology for obtaining global insights into developing transcriptome characteristics and elucidating the relative functional genes. A total of 54,255,544 high-quality reads were produced, trimmed, and de novo assembled into 115,281 contigs. 61,686 unigenes were obtained, with an average length of 1,009 nt. Among these unigenes, 17,348 were annotated into 59 Gene Ontology (GO) terms and 12,660 were assigned to 25 Cluster of Orthologous Groups classes, whereas 24,796 unigenes were mapped to 258 pathways. Differentially expressed unigenes between various developmental stages of C. formicarius were detected. Higher numbers of differentially expressed genes (DEGs) were recorded in the eggs versus L3 and eggs versus male samples (2,141 and 2,058 unigenes, respectively) than the others. Genes preferentially expressed in each stage were also identified. GO and pathway-based enrichment analysis were used to further investigate the functions of the DEGs. In addition, the expression profiles of ten DEGs were validated by quantitative real-time PCR. The transcriptome profiles presented in this study and these DEGs detected by comparative analysis of different developed stages of C. formicarius will facilitate the understanding of the molecular mechanism of various living process and will contribute to further genome-wide research.
authors.affiliation: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, 437 Dongguan Street, Baoding 071000, China (majuan_206@126.com; rongyanw@163.com; lixiuhua727@163.com; gaobo89@163.com; chenshulong65@163.com).
authors.forename: Juan
authors.initials: J
authors.lastname: Ma
authors.affiliation: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, 437 Dongguan Street, Baoding 071000, China (majuan_206@126.com; rongyanw@163.com; lixiuhua727@163.com; gaobo89@163.com; chenshulong65@163.com).
authors.forename: Rongyan
authors.initials: R
authors.lastname: Wang
authors.affiliation: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, 437 Dongguan Street, Baoding 071000, China (majuan_206@126.com; rongyanw@163.com; lixiuhua727@163.com; gaobo89@163.com; chenshulong65@163.com).
authors.forename: Xiuhua
authors.initials: X
authors.lastname: Li
authors.affiliation: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, 437 Dongguan Street, Baoding 071000, China (majuan_206@126.com; rongyanw@163.com; lixiuhua727@163.com; gaobo89@163.com; chenshulong65@163.com).
authors.forename: Bo
authors.initials: B
authors.lastname: Gao
authors.affiliation: Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture, 437 Dongguan Street, Baoding 071000, China (majuan_206@126.com; rongyanw@163.com; lixiuhua727@163.com; gaobo89@163.com; chenshulong65@163.com) chenshulong65@163.com.
authors.forename: Shulong
authors.initials: S
authors.lastname: Chen
mesh_terms: D000818:Animals; D001517:Coleoptera / Q000235:genetics* / Q000254:growth & development; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D008018:Life Cycle Stages / Q000235:genetics; D008297:Male; D059467:Transcriptome
keywords: different stages; differentially expressed genes; sweet potato weevil; transcriptome