title: Temporal and spatial regulation of mRNA export: Single particle RNA-imaging provides new tools and insights.
abstract: The transport of messenger RNAs (mRNAs) from the nucleus to cytoplasm is an essential step in the gene expression program of all eukaryotes. Recent technological advances in the areas of RNA-labeling, microscopy, and sequencing are leading to novel insights about mRNA biogenesis and export. This includes quantitative single molecule imaging (SMI) of RNA molecules in live cells, which is providing knowledge of the spatial and temporal dynamics of the export process. As this information becomes available, it leads to new questions, the reinterpretation of previous findings, and revised models of mRNA export. In this review, we will briefly highlight some of these recent findings and discuss how live cell SMI approaches may be used to further our current understanding of mRNA export and gene expression.
authors.affiliation: Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
authors.forename: Stephanie
authors.initials: S
authors.lastname: Heinrich
authors.affiliation: Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
authors.forename: Carina Patrizia
authors.initials: CP
authors.lastname: Derrer
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Canada.
authors.forename: Azra
authors.initials: A
authors.lastname: Lari
authors.affiliation: Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
authors.forename: Karsten
authors.initials: K
authors.lastname: Weis
authors.affiliation: Department of Cell Biology, University of Alberta, Edmonton, Canada.|Department of Viticulture and Enology, University of California, Davis, CA, USA.
authors.forename: Ben
authors.initials: B
authors.lastname: Montpetit
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D005786:Gene Expression Regulation; D006801:Humans; D022022:Nuclear Pore / Q000378:metabolism; D034443:RNA Transport; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism; D000072760:Single Molecule Imaging / Q000379:methods*
chemical_list: D012333:RNA, Messenger
keywords: MS2-MCP system; PP7-PCP system; RNA-binding protein; in vivo single molecule imaging; mRNA export; nuclear pore complex