title: Detecting N-RAS Q61R Mutated Thyroid Neoplasias by Immunohistochemistry.
abstract: Recently, the immunohistochemistry (IHC) for N-RAS Q61R has been developed and commercialized for clinical practice. Here, we investigated the reliability of IHC to identify N-RAS Q61R mutated thyroid neoplasia. A series of 24 consecutive thyroid lesions undergone surgery following indeterminate cytology were enrolled. Paraffin sections were stained for IHC using the rabbit monoclonal anti-human N-RAS Q61R, clone SP174. N-RAS mutations in codon 61 were also investigated by automated sequencing. At histology, 12 cases of follicular carcinoma, cytologically defined as follicular lesions, 1 papillary cancer, 7 follicular adenomas, and 4 hyperplastic nodules were found. Of these, 4 showed a positive IHC for anti N-RAS antibody where N-RAS expression was detected mainly at cytoplasmic level with similar intensity of reaction. The remaining cases had negative IHC. A 100% concordance between IHC and molecular analysis for N-RAS Q61R was observed. In conclusion, this study shows high reliability of IHC to identify N-RAS Q61R mutated thyroid lesions with high cost-effectiveness. These data indicate the reliability of IHC to identify N-RAS Q61R mutated thyroid neoplasia and suggest to adopt this approach for a more accurate management of patients, when indicated.
authors.affiliation: Section of Pathology, University Hospital Campus Bio Medico, Rome, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Crescenzi
authors.affiliation: Pathology Institute of Locarno, Locarno, Switzerland.
authors.forename: F
authors.initials: F
authors.lastname: Fulciniti
authors.affiliation: Institute of Pathology, University Hospital, Lausanne, Switzerland.
authors.forename: M
authors.initials: M
authors.lastname: Bongiovanni
authors.affiliation: Department of Nuclear Medicine and Thyroid Centre, Oncology Institute of Southern Switzerland, Bellinzona, Switzerland.
authors.forename: L
authors.initials: L
authors.lastname: Giovanella
authors.affiliation: Department of Nuclear Medicine and Thyroid Centre, Oncology Institute of Southern Switzerland, Bellinzona, Switzerland. pierpaolo.trimboli@gmail.com.
authors.forename: Pierpaolo
authors.initials: P
authors.lastname: Trimboli
mesh_terms: D000328:Adult; D000368:Aged; D004252:DNA Mutational Analysis / Q000379:methods*; D005260:Female; D020558:GTP Phosphohydrolases / Q000235:genetics*; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods*; D008297:Male; D008565:Membrane Proteins / Q000235:genetics*; D008875:Middle Aged; D015203:Reproducibility of Results; D013964:Thyroid Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: D008565:Membrane Proteins; D020558:GTP Phosphohydrolases; C579846:NRAS protein, human
keywords: Immunohistochemistry; Molecular analysis; N-RAS; Thyroid cancer