title: An antimicrobial peptide with an aggregation-induced emission (AIE) luminogen for studying bacterial membrane interactions and antibacterial actions.
abstract: A fluorescence technique to investigate the interactions between bacterial membranes and an aggregation-induced emission (AIE) luminogen-decorated AMP (TPE-AMP) was reported. Our simple and fast method consists of mixing TPE-AMP and bacterial suspensions and recording the fluorescence signals by flow cytometry and confocal microscopy in a "non-washing" manner.
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715, China. xulq@swu.edu.cn ecmli@swu.edu.cn.
authors.forename: Ning Ning
authors.initials: NN
authors.lastname: Li
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715, China. xulq@swu.edu.cn ecmli@swu.edu.cn.
authors.forename: Jun Zhi
authors.initials: JZ
authors.lastname: Li
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Peng
authors.initials: P
authors.lastname: Liu
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 117576, Singapore.
authors.forename: Dicky
authors.initials: D
authors.lastname: Pranantyo
authors.affiliation: College of Pharmaceutical Science, Southwest University, Chongqing, 400715, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Luo
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715, China. xulq@swu.edu.cn ecmli@swu.edu.cn.
authors.forename: Jiu Cun
authors.initials: JC
authors.lastname: Chen
authors.affiliation: Department of Chemical & Biomolecular Engineering, National University of Singapore, 117576, Singapore.
authors.forename: En-Tang
authors.initials: ET
authors.lastname: Kang
authors.affiliation: National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China. huxuefeng@scu.edu.cn.
authors.forename: Xue Feng
authors.initials: XF
authors.lastname: Hu
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715, China. xulq@swu.edu.cn ecmli@swu.edu.cn.
authors.forename: Chang Ming
authors.initials: CM
authors.lastname: Li
authors.affiliation: Institute for Clean Energy and Advanced Materials, Faculty of Materials and Energy, Southwest University, Chongqing, 400715, China. xulq@swu.edu.cn ecmli@swu.edu.cn.
authors.forename: Li Qun
authors.identifier: http://orcid.org/0000-0002-6780-114X
authors.initials: LQ
authors.lastname: Xu
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects / Q000633:toxicity; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects / Q000633:toxicity; D002462:Cell Membrane / Q000737:chemistry / Q000378:metabolism*; D004926:Escherichia coli / Q000187:drug effects; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects / Q000633:toxicity; D008689:Methacrylates / Q000737:chemistry / Q000378:metabolism* / Q000528:radiation effects / Q000633:toxicity; D008813:Mice, Inbred ICR; D008826:Microbial Sensitivity Tests; D011485:Protein Binding; D011550:Pseudomonas aeruginosa / Q000187:drug effects; D013211:Staphylococcus aureus / Q000187:drug effects; D013212:Staphylococcus epidermidis / Q000187:drug effects; D014466:Ultraviolet Rays
chemical_list: D000900:Anti-Bacterial Agents; D023181:Antimicrobial Cationic Peptides; D005456:Fluorescent Dyes; D008689:Methacrylates