title: Activity of Pericampylus glaucus and periglaucine A in vitro against nasopharangeal carcinoma and anti-inflammatory activity.
abstract: BACKGROUND
  Pericampylus glaucus is a climbing plant found across Asia and used in traditional medicine to treat a number of conditions including splenomegaly, fever, cough, laryngitis, pulmonary disease, asthma, headache, hair loss, snake bite, boar bite, factures, boils, tumours, tetanus, rheumatic pain, itches and eclampsia.
  
  
  OBJECTIVE
  To test extracts of P. glaucus in a number of bioassays and determine the legitimacy of its traditional use.
  
  
  METHODS
  The stems, leaves, roots and fruits of P. glaucus were collected and extracted sequentially with hexane, chloroform and ethanol, respectively. The anti-inflammatory activity was assessed by testing the ability of the extracts to inhibit heat induced protein denaturation, stabilise human red blood cells under hypotonic stress and by testing the inhibitory activity of the extracts against cyclooxygenases 1 and 2. Cytotoxicity was tested using the human lung epithelial cell line MRC-5 and nasopharangeal carcinoma cell line HK1 in the MTT assay.
  
  
  RESULTS
  Many of the samples showed an ability to prevent heat induced protein denaturation, as well as prevent lysis of red blood cells. Most of the extracts demonstrated inhibitory activity towards both of the COX enzymes. The ethanol extracts tended to demonstrate greater toxicity than other extracts, with some of the other extracts significantly enhancing growth and metabolism of the cells.
  
  
  CONCLUSIONS
  The benefit of P. glaucus for the treatment of diseases related to inflammation and cancer was supported by the in vitro assays adopted in this study.
authors.affiliation: School of Pharmacy, Faculty of Science, The University of Nottingham Malaysia Campus, Semenyih, Selangor, Malaysia. Electronic address: khyx3fnp@nottingham.edu.my.
authors.forename: Fiona Natalia
authors.initials: FN
authors.lastname: Shipton
authors.affiliation: School of Pharmacy, Faculty of Science, The University of Nottingham Malaysia Campus, Semenyih, Selangor, Malaysia.
authors.forename: Teng-Jin
authors.initials: TJ
authors.lastname: Khoo
authors.affiliation: School of Pharmacy, Faculty of Science, The University of Nottingham Malaysia Campus, Semenyih, Selangor, Malaysia.
authors.forename: Md Shahadat
authors.initials: MS
authors.lastname: Hossan
authors.affiliation: School of Pharmacy, Faculty of Science, The University of Nottingham Malaysia Campus, Semenyih, Selangor, Malaysia. Electronic address: Christophe.Wiart@nottingham.edu.my.
authors.forename: Christophe
authors.initials: C
authors.lastname: Wiart
mesh_terms: D000470:Alkaloids / Q000494:pharmacology*; D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D000972:Antineoplastic Agents, Phytogenic / Q000494:pharmacology*; D045744:Cell Line, Tumor; D016861:Cyclooxygenase Inhibitors / Q000494:pharmacology; D004365:Drugs, Chinese Herbal / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects; D006801:Humans; D009303:Nasopharyngeal Neoplasms / Q000188:drug therapy* / Q000473:pathology; D010936:Plant Extracts / Q000494:pharmacology*; D010946:Plants, Medicinal; D011489:Protein Denaturation
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D000972:Antineoplastic Agents, Phytogenic; D016861:Cyclooxygenase Inhibitors; D004365:Drugs, Chinese Herbal; D010936:Plant Extracts; C000621429:periglaucine A
keywords: Alkaloid; Anti-inflammatory; Cancer; Cyclooxygenase; Cytotoxicity; Pericampylus glaucus; Periglaucine A (PubChem CID: 24879468)