title: Assessment of membrane stabilizing activity from honey. An in-vitro approach.
abstract: BACKGROUND
  esent study was conducted to evaluate Manoflora (MF), Polyflora (PF), Polyflora forest (PFf), and Processed (Pro) honey varieties to compare the in-vitro anti-inflammatory effects of aqueous honey samples in dose dependent manner. In-vitro anti-inflammatory activity was evaluated using membrane stabilization assay of RBCs at different aqueous honey concentrations. Material and method. The present investigation carried out for selected varieties of honey against erythrocytes exposed to both heat and hypotonic lyses and inhibition of membrane damage was compared to the standard drug acetylsalicylic acid. Results. Membrane damage was inhibited in both the model hemolysis of erythrocytes in vitro in a concentration dependent manner. Hypotonic solution inducing damage was inhibited by aqueous honey sample in ascending order ranged from 8.25% to 97.76% at 10 to 50 mg/ml and standard drug acetylsalicylic acid showing hemolysis protection 96.09% at 100 μg/ml concentration. In heat induced hemolysis model aqueous honey sample exhibited its protecting property during external stress condition in all samples ranged from 0.44% to 21.23% at 10 to 50 mg/ml and acetylsalicylic acid showed 39.38% at 100 μg/ml concentration. Among the variety PFf showed highest protecting nature for hypotonic solution induced lyses (97.76%) and heat induced hemolysis (21.23%) at 50 mg/ml respectively. Conclusion. With these investigations data conclude that the model exhibits marked anti-inflammatory effect. Future research is to be carried out to identify the molecules responsible in honey and its mechanism.
  
  
  METHODS
  present study was conducted to evaluate Manoflora (MF), Polyflora (PF), Polyflora forest (PFf), and Processed (Pro) honey varieties to compare the in-vitro anti-inflammatory effects of aqueous honey samples in dose dependent manner. In-vitro anti-inflammatory activity was evaluated using membrane stabilization assay of RBCs at different aqueous honey concentrations. Material and method. The present investigation carried out for selected varieties of honey against erythrocytes exposed to both heat and hypotonic lyses and inhibition of membrane damage was compared to the standard drug acetylsalicylic acid.
  
  
  RESULTS
  Membrane damage was inhibited in both the model hemolysis of erythrocytes in vitro in a concentration dependent manner. Hypotonic solution inducing damage was inhibited by aqueous honey sample in ascending order ranged from 8.25% to 97.76% at 10 to 50 mg/ml and standard drug acetylsalicylic acid showing hemolysis protection 96.09% at 100 μg/ml concentration. In heat induced hemolysis model aqueous honey sample exhibited its protecting property during external stress condition in all samples ranged from 0.44% to 21.23% at 10 to 50 mg/ml and acetylsalicylic acid showed 39.38% at 100 μg/ml concentration. Among the variety PFf showed highest protecting nature for hypotonic solution induced lyses (97.76%) and heat induced hemolysis (21.23%) at 50 mg/ml respectively.
  
  
  CONCLUSIONS
  With these investigations data conclude that the model exhibits marked anti-inflammatory effect. Future research is to be carried out to identify the molecules responsible in honey and its mechanism involved.
authors.affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangothri, Karnataka, India.
authors.forename: Honnayakanahalli Marichenne Gowda
authors.initials: HM
authors.lastname: Manukumar
authors.affiliation: Department of Studies in Biotechnology, University of Mysore, Manasagangothri, Karnataka, India.
authors.forename: Sharanaiah
authors.initials: S
authors.lastname: Umesha
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology*; D004910:Erythrocyte Membrane / Q000187:drug effects*; D004912:Erythrocytes / Q000187:drug effects; D006461:Hemolysis / Q000187:drug effects*; D006722:Honey; D006358:Hot Temperature; D006801:Humans; D007038:Hypotonic Solutions
chemical_list: D000893:Anti-Inflammatory Agents; D007038:Hypotonic Solutions
keywords: anti-inflammatory; erythrocytes; hemolysis; honey; hypotonic