title: Simultaneous Determination of Four Triterpenoid Saponins in Aralia elata Leaves by HPLC-ELSD Combined with Hierarchical Clustering Analysis.
abstract: BACKGROUND
  Aralia elata leaves are known to have several biological activities, including anti-arrythmia, antitumor, anti-inflammatory, anti-fatigue, antimicrobial and antiviral effects. Our previous study found that triterpenoid saponins from the leaves of A. elata had antitumor effects. Quantification of the triterpenoids is important for the quality control of A. elata leaves.
  
  
  OBJECTIVE
  To establish high-performance liquid chromatography coupled with evaporative light scattering detection (HPLC-ELSD) for the simultaneous determination of four major triterpenoid saponins, including Aralia-saponin IV, Aralia-saponin VI, 3-O-β-d- glucopyranosyl-(1 → 3)-β-d-glucopyranosyl-(1 → 3)-β-d-glucopyranosyl oleanolic acid 28-O-β-d-glucopyranoside (Aralia-saponin TTP)and Aralia-saponin V.
  
  
  METHODS
  The separation was carried out on a Dikma Diamonsil C18column (4.6 mm × 250 mm, 5 μm) efficiently with gradient elution consisting of acetonitrile and water.
  
  
  RESULTS
  All calibration curves showed good linear regression (R2> 0.9996) within the ranges of tested concentrations. This validated method was applied to determine the contents of the four major triterpenoid saponins in 53 samples from different regions of northeast China. Hierarchical clustering analysis was first used to classify and differentiate Aralia elata leaves.
  
  
  CONCLUSIONS
  The method developed was successfully applied to analyse four major triterpenoid saponins in Aralia elata leaves which is helpful for quality control of the herb. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Yichun
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Baimei
authors.initials: B
authors.lastname: Li
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Xiaoting
authors.initials: X
authors.lastname: Lin
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Xue
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Zhibin
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Hongwei
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Hai
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chinese Materia Medica (Ministry of Education), Heilongjiang University of Chinese Medicine, Harbin, 150040, China.
authors.forename: Haixue
authors.initials: H
authors.lastname: Kuang
mesh_terms: D027983:Aralia / Q000737:chemistry*; D002138:Calibration; D005591:Chemical Fractionation; D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D016000:Cluster Analysis; D018515:Plant Leaves / Q000737:chemistry*; D011786:Quality Control; D015203:Reproducibility of Results; D012503:Saponins / Q000032:analysis* / Q000737:chemistry; D012680:Sensitivity and Specificity
chemical_list: D012503:Saponins
keywords: Aralia elata leaves; HPLC-ELSD; hierarchical clustering analysis; triterpenoid saponins