title: Novel functionalized poly(glycidyl methacrylate-co-ethylene dimethacrylate) microspheres for the solid-phase extraction of glycopeptides/glycoproteins.
abstract: Novel 3-aminophenylboronic acid functionalized poly(glycidyl methacrylate-co-ethylene dimethacrylate) microspheres were prepared for the solid-phase extraction of glycopeptides/glycoproteins. The adsorption efficiency, maximum adsorption capacity, and specific recognition of the microspheres to glycoprotein were investigated. The results indicated excellent adsorption of glycoproteins by the microspheres, which are attributed to the well-defined boronic acid brushes on the microsphere surfaces. Furthermore, a solid-phase extraction microcolumn filled with the microspheres was used to efficiently enrich glycopeptides from enzymatic hydrolysates from human serum samples. The mass spectrometry results demonstrated that the method is suitable for the separation and enrichment of glycopeptides/glycoproteins from complex biological samples.
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical engineering, Shaanxi Normal University, Xi'an, China.|Institute of Sports Biology, School of Physical Education, Shaanxi Normal University, Xi'an, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical engineering, Shaanxi Normal University, Xi'an, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Qi
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical engineering, Shaanxi Normal University, Xi'an, China.
authors.forename: Wei-Ting
authors.initials: WT
authors.lastname: Zheng
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical engineering, Shaanxi Normal University, Xi'an, China.
authors.forename: Yong-Le
authors.initials: YL
authors.lastname: Tian
authors.affiliation: Institute of Sports Biology, School of Physical Education, Shaanxi Normal University, Xi'an, China.
authors.forename: Ai-Ping
authors.initials: AP
authors.lastname: Chi
authors.affiliation: Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical engineering, Shaanxi Normal University, Xi'an, China.
authors.forename: Zhi-Qi
authors.initials: ZQ
authors.lastname: Zhang
mesh_terms: D006020:Glycopeptides / Q000097:blood / Q000302:isolation & purification*; D006801:Humans; D008768:Methylmethacrylates; D008863:Microspheres; D052616:Solid Phase Extraction
chemical_list: D006020:Glycopeptides; D008768:Methylmethacrylates; C020347:GMA-EDMA
keywords: 3-aminophenylboronic acid; glycoproteins; microspheres; solid-phase extraction