title: Inhibiting effects of rhynchophylline on methamphetamine-dependent zebrafish are related with the expression of tyrosine hydroxylase (TH).
abstract: In this study, to study the effect of rhynchophylline on TH in midbrain of methamphetamine-induced conditioned place preference (CPP) adult zebrafish, place preference adult zebrafish models were established by methamphetamine (40μg/g) and the expression of TH was observed by immunohistochemistry technique and Western blot. Ketamine (150μg/g), high dose of rhynchophylline (100μg/g) group can significantly reduce the place preference; immunohistochemistry results showed that the number of TH-positive neurons in midbrain was increased in the methamphetamine model group, whereas less TH-positive neurons were found in the ketamine group and high dosage rhynchophylline group. Western blot results showed that the expression of TH protein was significantly increased in the model group, whereas less expression was found in the ketamine group, high dosage rhynchophylline group. Our data pointed out that TH plays an important role in the formation of methamphetamine-induced place preference in adult zebrafish. Rhynchophylline reversed the expression of TH in the midbrain demonstrates the potential effect of mediates methamphetamine induced rewarding effect.
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Zhu
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Chaohua
authors.initials: C
authors.lastname: Luo
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Liu
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Chan
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Fang
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Yingbo
authors.initials: Y
authors.lastname: Lin
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Jinying
authors.initials: J
authors.lastname: Ou
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Minting
authors.initials: M
authors.lastname: Chen
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China.
authors.forename: Daoqi
authors.initials: D
authors.lastname: Zhu
authors.affiliation: Department of Biology, Hong Kong Baptist University, Kowloon Tong, Hong Kong.
authors.forename: Ken Kin-Lam
authors.initials: KK
authors.lastname: Yung
authors.affiliation: School of Traditional Chinese Medicine, Southern Medical University, Guangdong, Guangzhou 510515, China. Electronic address: cherrymo@fimmu.com.
authors.forename: Zhixian
authors.initials: Z
authors.lastname: Mo
mesh_terms: D019969:Amphetamine-Related Disorders; D000818:Animals; D001522:Behavior, Animal / Q000187:drug effects; D003216:Conditioning, Operant / Q000187:drug effects*; D026121:Indole Alkaloids / Q000494:pharmacology*; D007649:Ketamine / Q000494:pharmacology; D008297:Male; D008636:Mesencephalon / Q000166:cytology / Q000201:enzymology; D008694:Methamphetamine / Q000494:pharmacology*; D009474:Neurons / Q000201:enzymology; D000078183:Oxindoles; D014446:Tyrosine 3-Monooxygenase / Q000378:metabolism*; D015027:Zebrafish
chemical_list: D026121:Indole Alkaloids; D000078183:Oxindoles; D008694:Methamphetamine; C052714:rhyncophylline; D007649:Ketamine; D014446:Tyrosine 3-Monooxygenase
keywords: Ketamine hydrochloride (PubChem CID: 15851); Methamphetamine dependence; Methamphetamine hydrochloride (PubChem CID: 10836); Rhynchophylline; Rhynchophylline (PubChem CID: 5281408); Tyrosine hydroxylase (TH)