title: Purification and characterization of bioactive peptides RYamide and CCHamide in the kuruma shrimp Marsupenaeus japonicus.
abstract: To understand the regulation systems of appetite, bioactive peptides from the kuruma shrimp Marsupenaeus japonicus (Mj) were isolated and purified by reverse pharmacological assays using CHO cells expressing the Drosophila melanogaster G-protein-coupled receptors (GPCRs) CG5811 (a RYamide receptor) or CG14593 (a CCHamide-2 receptor). Four peptides having binding activity to GPCRs were obtained and named Mj RYamide-1, Mj RYamide-2, Mj RYamide-3, and Mj CCHamide. Genes encoding the prepropeptides of these peptides were identified using kuruma shrimp transcriptome databases. The Mj prepro-RYamide gene encodes a 130-amino acid polypeptide containing Mj RYamide-1, Mj RYamide-2, and Mj RYamide-3, whereas the Mj prepro-CCHamide gene encodes a 119-amino acid polypeptide containing a single Mj CCHamide peptide. The expression of these genes was confirmed in various neuronal organs including the brain and ventral nerve cord. In addition, prepro-RYamide gene expression is significantly reduced in the brain after starvation. RYamides may thus be associated with regulation of feeding or digestion. Changes in kayak (the c-fos ortholog in invertebrates) gene expression after administration of synthetic peptides were also investigated. Mj kayak expression levels are upregulated in hepatopancreas after treatment with Mj RYamide-3 or CCHamide. Thus, the peptides isolated in this study may have some regulatory effect on cellular metabolism in aquacultured invertebrates.
authors.affiliation: National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Mie 516-0193, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Mekata
authors.affiliation: Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki, Miyazaki 889-2192, Japan.
authors.forename: Tomoya
authors.initials: T
authors.lastname: Kono
authors.affiliation: National Research Institute of Aquaculture, Japan Fisheries Research and Education Agency, Mie 516-0193, Japan.
authors.forename: Jun
authors.initials: J
authors.lastname: Satoh
authors.affiliation: Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Osaka 565-8565, Japan.
authors.forename: Morikatsu
authors.initials: M
authors.lastname: Yoshida
authors.affiliation: Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Osaka 565-8565, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Mori
authors.affiliation: Molecular Genetics, Institute of Life Sciences, Kurume University, Fukuoka 839-0864, Japan.
authors.forename: Takahiro
authors.initials: T
authors.lastname: Sato
authors.affiliation: Department of Biochemistry, National Cerebral and Cardiovascular Center Research Institute, Osaka 565-8565, Japan.
authors.forename: Mikiya
authors.initials: M
authors.lastname: Miyazato
authors.affiliation: Division of Searching and Identification of Bioactive Peptides, Department of Bioactive Peptides, Frontier Science Research Center, University of Miyazaki, Miyazaki 889-1692, Japan; Division of Research & Inspection for Infectious Diseases, Center for Animal Disease Control, University of Miyazaki, Miyazaki 889-1692, Japan. Electronic address: a0d203u@cc.miyazaki-u.ac.jp.
authors.forename: Takanori
authors.initials: T
authors.lastname: Ida
mesh_terms: D000818:Animals; D004330:Drosophila / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics / Q000378:metabolism*; D009479:Neuropeptides / Q000378:metabolism*; D033561:Penaeidae / Q000378:metabolism*; D010455:Peptides / Q000235:genetics*; D017476:Receptors, Neuropeptide Y / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: C555823:CCH-amide-2 neuropeptide, Drosophila; D029721:Drosophila Proteins; D009479:Neuropeptides; D010455:Peptides; C561244:RYa-R protein, Drosophila; D017476:Receptors, Neuropeptide Y
keywords: Appetite regulation; CCHamide; RYamide; Shrimp; Tissue distribution