title: Effect of Trehalose and Trehalose Transport on the Tolerance ofClostridium perfringensto Environmental Stress in a Wild Type Strain and Its Fluoroquinolone-Resistant Mutant.
abstract: Trehalose has been shown to protect bacterial cells from environmental stress. Its uptake and osmoprotective effect inClostridium perfringenswere investigated by comparing wild typeC. perfringensATCC 13124 with a fluoroquinolone- (gatifloxacin-) resistant mutant. In a chemically defined medium, trehalose and sucrose supported the growth of the wild type but not that of the mutant. Microarray data and qRT-PCR showed that putative genes for the phosphorylation and transport of sucrose and trehalose (via phosphoenolpyruvate-dependent phosphotransferase systems, PTS) and some regulatory genes were downregulated in the mutant. The wild type had greater tolerance than the mutant to salts and low pH; trehalose and sucrose further enhanced the osmotolerance of the wild type to NaCl. Expression of the trehalose-specific PTS was lower in the fluoroquinolone-resistant mutant. Protection ofC. perfringensfrom environmental stress could therefore be correlated with the ability to take up trehalose.
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA.
authors.forename: Miseon
authors.initials: M
authors.lastname: Park
authors.affiliation: School of Life Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK.
authors.forename: Wilfrid J
authors.initials: WJ
authors.lastname: Mitchell
authors.affiliation: Division of Microbiology, National Center for Toxicological Research, FDA, Jefferson, AR 72079, USA.
authors.forename: Fatemeh
authors.identifier: 0000-0001-9905-1569
authors.initials: F
authors.lastname: Rafii