title: Feasibility of reusing the black liquor for enzymatic hydrolysis and ethanol fermentation.
abstract: The black liquor (BL) generated in the alkaline pretreatment process is usually thought as the environmental pollutant. This study found that the pure alkaline lignin hardly inhibited the enzymatic hydrolysis of cellulose (EHC), which led to the investigation on the feasibility of reusing BL as the buffer via pH adjustment for the subsequent enzymatic hydrolysis and fermentation. The pH value of BL was adjusted from 13.23 to 4.80 with acetic acid, and the alkaline lignin was partially precipitated. It deposited on the surface of cellulose and negatively influenced the EHC via blocking the access of cellulase to cellulose and adsorbing cellulase. The supernatant separated from the acidified BL scarcely affected the EHC, but inhibited the ethanol fermentation. The 4-times diluted supernatant and the last-time waste wash water of the alkali-treated sugarcane bagasse didn't inhibit the EHC and ethanol production. This work gives a clue of saving water for alkaline pretreatment.
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Wang
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Chen
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Xuesong
authors.initials: X
authors.lastname: Tan
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Yunyun
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Minchao
authors.initials: M
authors.lastname: He
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Yu
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Qi
authors.affiliation: Bureau of Environmental Protection of Shuangtaizi District, Panjin 124000, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China. Electronic address: zhuangxs@ms.giec.ac.cn.
authors.forename: Xinshu
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China; Collaborative Innovation Center of Biomass Energy, Zhengzhou 450002, China.
authors.forename: Zhenhong
authors.initials: Z
authors.lastname: Yuan
mesh_terms: D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D000431:Ethanol / Q000032:analysis / Q000378:metabolism*; D005285:Fermentation; D006868:Hydrolysis; D008031:Lignin / Q000378:metabolism*; D012037:Refuse Disposal / Q000379:methods*; D031786:Saccharum / Q000378:metabolism
chemical_list: D000431:Ethanol; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D002480:Cellulase
keywords: Adsorption; Alkaline pretreatment; Lignin; Steric hindrance; Sugarcane bagasse