title: Prostate cancer proteomics: Current trends and future perspectives for biomarker discovery.
abstract: The clinical and fundamental research in prostate cancer - the most common urological cancer in men - is currently entering the proteomic and genomic era. The focus has switched from one single marker (PSA) to panels of biomarkers (including proteins involved in ribosomal function and heat shock proteins). Novel genetic markers (such as Transmembrane protease serine 2 (TMPRSS2)-ERG fusion gene mRNA) or prostate cancer gene 3 (PCA3) had already entered the clinical practice, raising the question whether subsequent protein changes impact the evolution of the disease and the response to treatment. Proteomic technologies such as MALDI-MS, SELDI-MS, i-TRAQ allow a qualitative/quantitative analysis of the proteome variations, in both serum and tumor tissue. A new trend in prostate cancer research is proteomic analysis of prostasomes (prostate-specific exosomes), for the discovery of new biomarkers. This paper provides an update of novel clinical tests used in research and clinical diagnostic, as well as of potential tissue or fluid biomarkers provided by extensive proteomic research data.
authors.affiliation: Department of Biochemistry-Proteomics, Victor Babes National Institute of Pathology, Bucharest, Romania.|Titu Maiorescu University, Faculty of Medicine, Bucharest, Romania.
authors.forename: Cristiana Pistol
authors.initials: CP
authors.lastname: Tanase
authors.affiliation: Department of Biochemistry-Proteomics, Victor Babes National Institute of Pathology, Bucharest, Romania.
authors.forename: Elena
authors.initials: E
authors.lastname: Codrici
authors.affiliation: Department of Biochemistry-Proteomics, Victor Babes National Institute of Pathology, Bucharest, Romania.
authors.forename: Ionela Daniela
authors.initials: ID
authors.lastname: Popescu
authors.affiliation: Department of Biochemistry-Proteomics, Victor Babes National Institute of Pathology, Bucharest, Romania.
authors.forename: Simona
authors.initials: S
authors.lastname: Mihai
authors.affiliation: Department of Biochemistry-Proteomics, Victor Babes National Institute of Pathology, Bucharest, Romania.|Department of Cell Biology and Histology, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.
authors.forename: Ana-Maria
authors.initials: AM
authors.lastname: Enciu
authors.affiliation: Department of Biochemistry-Proteomics, Victor Babes National Institute of Pathology, Bucharest, Romania.|Stefan S Nicolau Institute of Virology, Bucharest, Romania.
authors.forename: Laura Georgiana
authors.initials: LG
authors.lastname: Necula
authors.affiliation: Center for Uronephrology and Renal Transplantation, Fundeni Clinical Institute, Bucharest, Romania.
authors.forename: Adrian
authors.initials: A
authors.lastname: Preda
authors.affiliation: Center of Internal Medicine-Nephrology, Fundeni Clinical Institute, Bucharest, Romania.|Carol Davila University of Medicine and Pharmacy, Faculty of Medicine, Bucharest, Romania.
authors.forename: Gener
authors.initials: G
authors.lastname: Ismail
authors.affiliation: Department of Biochemistry-Proteomics, Victor Babes National Institute of Pathology, Bucharest, Romania.|National Institute for Chemical Pharmaceutical R&D, Bucharest, Romania.
authors.forename: Radu
authors.initials: R
authors.lastname: Albulescu
mesh_terms: D014408:Biomarkers, Tumor / Q000378:metabolism*; D006801:Humans; D008297:Male; D009363:Neoplasm Proteins / Q000378:metabolism*; D011471:Prostatic Neoplasms / Q000378:metabolism* / Q000473:pathology; D040901:Proteomics / Q000379:methods; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor; D009363:Neoplasm Proteins
keywords: biomarkers; mass spectrometry; prostasomes; prostate cancer; proteomics