title: Development of a reversible fluorescent probe for reactive sulfur species, sulfane sulfur, and its biological application.
abstract: We report a reversible off/on fluorescent probe for monitoring concentration changes of sulfane sulfur by utilizing the unique ability of sulfane sulfur to bind reversibly to other sulfur atoms and the intramolecular spirocyclization reaction of xanthene dyes. It reversibly visualized sulfane sulfur in living A549 cells and primary-cultured hippocampal astrocytes.
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp.
authors.forename: Yoko
authors.identifier: http://orcid.org/0000-0002-3870-4818
authors.initials: Y
authors.lastname: Takano
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Hanaoka
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp.
authors.forename: Kazuhito
authors.initials: K
authors.lastname: Shimamoto
authors.affiliation: Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Miyamoto
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp and PRESTO, Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Komatsu
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp.
authors.forename: Tasuku
authors.initials: T
authors.lastname: Ueno
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp.
authors.forename: Takuya
authors.identifier: http://orcid.org/0000-0002-3425-3589
authors.initials: T
authors.lastname: Terai
authors.affiliation: Department of Molecular Pharmacology, National Institute of Neuroscience, National Center of Neurology and Psychiatry, 4-1-1 Ogawa-Higashi, Kodaira, Tokyo 187-8502, Japan.
authors.forename: Hideo
authors.initials: H
authors.lastname: Kimura
authors.affiliation: Drug Discovery Initiative, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Nagano
authors.affiliation: Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. uranokun@m.u-tokyo.ac.jp khanaoka@mol.f.u-tokyo.ac.jp and Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan and AMED CREST (Japan) Agency for Medical Research and Development 1-7-1 Otemachi, Chiyoda-ku, Tokyo 100-0004, Japan.
authors.forename: Yasuteru
authors.initials: Y
authors.lastname: Urano
mesh_terms: D000072283:A549 Cells; D001253:Astrocytes / Q000737:chemistry; D005456:Fluorescent Dyes / Q000032:analysis* / Q000138:chemical synthesis / Q000737:chemistry*; D006624:Hippocampus / Q000737:chemistry; D006801:Humans; D015394:Molecular Structure; D013457:Sulfur Compounds / Q000032:analysis* / Q000737:chemistry*
chemical_list: D005456:Fluorescent Dyes; D013457:Sulfur Compounds