title: Oxidative Stress and Genotoxicity of Zinc Oxide Nanoparticles to Pseudomonas Species, Human Promyelocytic Leukemic (HL-60), and Blood Cells.
abstract: In the present study, toxicity of commercial zinc oxide nanoparticles (ZnO NPs) was studied on the bacterium Pseudomonas sp., human promyelocytic leukemia (HL-60) cells, and peripheral blood mononuclear cells (PBMC). The toxicity was assessed by measuring growth, cell viability, and protein expression in bacterial cell. The bacterial growth and viability decreased with increasing concentrations of ZnO NP. Three major proteins, ribosomal protein L1 and L9 along with alkyl hydroperoxides reductase, were upregulated by 1.5-, 1.7-, and 2.0-fold, respectively, after ZnO NP exposure. The results indicated oxidative stress as the leading cause of toxic effect in bacteria. In HL-60 cells, cytotoxic and genotoxic effects along with antioxidant enzyme activity and reactive oxygen species (ROS) generation were studied upon ZnO NP treatment. ZnO NP exhibited dose-dependent increase in cell death after 24-h exposure. The DNA-damaging potential of ZnO NP in HL-60 cells was maximum at 0.05 mg/L concentration. Comet assay showed 70-80% increase in tail DNA at 0.025 to 0.05 mg/L ZnO NP concentration. A significant increase of 1.6-, 1.4-, and 2.0-fold in ROS level was observed after 12 h. Genotoxic potential of ZnO NPs was also demonstrated in PBMC through DNA fragmentation. Thus, ZnO NP, besides being an essential element having antibacterial activity, also showed toxicity towards human cells (HL-60 and PBMC).
authors.affiliation: Environmental Biotechnology Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, 440020, India.
authors.forename: Deepika
authors.initials: D
authors.lastname: Soni
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, 440020, India.
authors.forename: Deepa
authors.initials: D
authors.lastname: Gandhi
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, 440020, India.
authors.forename: Prashant
authors.initials: P
authors.lastname: Tarale
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, 440020, India.
authors.forename: Amit
authors.initials: A
authors.lastname: Bafana
authors.affiliation: Environmental Biotechnology Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, 440020, India.
authors.forename: R A
authors.initials: RA
authors.lastname: Pandey
authors.affiliation: Environmental Health Division, CSIR-National Environmental Engineering Research Institute (NEERI), Nagpur, 440020, India. ss_devi@neeri.res.in.
authors.forename: Saravanadevi
authors.initials: S
authors.lastname: Sivanesan
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology; D004249:DNA Damage; D018922:HL-60 Cells; D006801:Humans; D007963:Leukocytes, Mononuclear / Q000378:metabolism*; D053758:Nanoparticles; D018384:Oxidative Stress / Q000187:drug effects*; D011549:Pseudomonas / Q000254:growth & development*; D015034:Zinc Oxide / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D015034:Zinc Oxide
keywords: Cytotoxicity; DNA damage; MTT; Protein expression; ROS; ZnO NP