title: Determining Substrate Specificities of β1,4-Endogalactanases Using Plant Arabinogalactan Oligosaccharides Synthesized by Automated Glycan Assembly.
abstract: Pectin is a structurally complex plant polysaccharide with many industrial applications in food products. The structural elucidation of pectin is aided by digestion assays with glycosyl hydrolases. We report the automated glycan assembly of oligosaccharides related to the arabinogalactan side chains of pectin as novel biochemical tools to determine the substrate specificities of endogalactanases. Analysis of the digestion products revealed different requirements for the lengths and arabinose substitution pattern of the oligosaccharides to be recognized and hydrolyzed by the galactanases.
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces , Am Mühlenberg 1, 14476 Potsdam, Germany.|Institute of Chemistry and Biochemistry, Freie Universität Berlin , Arnimallee 22, 14195 Berlin, Germany.
authors.forename: Max P
authors.initials: MP
authors.lastname: Bartetzko
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces , Am Mühlenberg 1, 14476 Potsdam, Germany.|Institute of Chemistry and Biochemistry, Freie Universität Berlin , Arnimallee 22, 14195 Berlin, Germany.
authors.forename: Frank
authors.identifier: 0000-0002-2586-4118
authors.initials: F
authors.lastname: Schuhmacher
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces , Am Mühlenberg 1, 14476 Potsdam, Germany.|Institute of Chemistry and Biochemistry, Freie Universität Berlin , Arnimallee 22, 14195 Berlin, Germany.
authors.forename: Peter H
authors.initials: PH
authors.lastname: Seeberger
authors.affiliation: Department of Biomolecular Systems, Max Planck Institute of Colloids and Interfaces , Am Mühlenberg 1, 14476 Potsdam, Germany.|Institute of Chemistry and Biochemistry, Freie Universität Berlin , Arnimallee 22, 14195 Berlin, Germany.
authors.forename: Fabian
authors.identifier: 0000-0003-2206-6636
authors.initials: F
authors.lastname: Pfrengle
mesh_terms: D001331:Automation; D005685:Galactans / Q000737:chemistry*; D006026:Glycoside Hydrolases / Q000378:metabolism*; D015394:Molecular Structure; D011134:Polysaccharides / Q000096:biosynthesis* / Q000737:chemistry; D013379:Substrate Specificity
chemical_list: D005685:Galactans; D011134:Polysaccharides; D006026:Glycoside Hydrolases; C094192:arabinogalactan endo-1,4-beta-galactosidase; C005653:arabinogalactan