title: Analysis of the Damage Mechanism Related to CO2Laser Cochleostomy on Guinea Pig Cochlea.
abstract: Different types of lasers have been used in inner ear surgery. Therefore, it is of the utmost importance to avoid damage to the inner ear (e.g., hyperthermia and acoustic effects) caused by the use of such lasers. The aim of this study was to use a high powered fibre-enabled CO2laser (10 W, 606 J/cm2) to perform cochleostomies on guinea pig cochlea and to investigate the possible laser-induced damage mechanisms. The temperature changes in the round window membrane, auditory evoked brainstem response, and morphological of the hair cells were measured and recorded before and after laser application. All of the outcomes differed in comparison with the control group. A rise in temperature and subsequent increased hearing loss were observed in animals that underwent surgery with a 10 W CO2laser. These findings correlated with increased injury to the cochlear ultrastructure and a higher positive expression of E-cadherin andβ-catenin in the damaged organ of Corti. We assume that enhanced cell-cell adhesion and the activatedβ-catenin-related canonical Wnt-signalling pathway may play a role in the protection of the cochlea to prevent further damage.
authors.affiliation: Department of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, China; Shanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, China; Key Laboratory of Hearing Medicine, Ministry of Health, Shanghai 200031, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, China; Shanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, China; Key Laboratory of Hearing Medicine, Ministry of Health, Shanghai 200031, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Qian
authors.affiliation: Shanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, China; Key Laboratory of Hearing Medicine, Ministry of Health, Shanghai 200031, China; Department of Research Center, EYE & ENT Hospital of Fudan University, Shanghai 200031, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Ma
authors.affiliation: Department of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, China; Shanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, China; Key Laboratory of Hearing Medicine, Ministry of Health, Shanghai 200031, China.
authors.forename: Fang-Lu
authors.identifier: 0000-0002-3243-0393
authors.initials: FL
authors.lastname: Chi
authors.affiliation: Department of Otology & Skull Base Surgery, EYE & ENT Hospital of Fudan University, Shanghai 200031, China; Shanghai Clinical Medical Center of Hearing Medicine, Shanghai 200031, China; Key Laboratory of Hearing Medicine, Ministry of Health, Shanghai 200031, China.
authors.forename: Dong-Dong
authors.identifier: 0000-0002-2889-9375
authors.initials: DD
authors.lastname: Ren
mesh_terms: D000818:Animals; D001933:Brain Stem / Q000473:pathology / Q000503:physiopathology; D003051:Cochlea / Q000473:pathology* / Q000503:physiopathology / Q000601:surgery*; D016057:Evoked Potentials, Auditory, Brain Stem / Q000502:physiology; D006168:Guinea Pigs; D034381:Hearing Loss / Q000209:etiology / Q000473:pathology* / Q000503:physiopathology; D054020:Lasers, Gas / Q000009:adverse effects*; D008297:Male