title: Sororin is enriched at the central region of synapsed meiotic chromosomes.
abstract: During meiotic prophase, cohesin complexes mediate cohesion between sister chromatids and promote pairing and synapsis of homologous chromosomes. Precisely how the activity of cohesin is controlled to promote these events is not fully understood. In metazoans, cohesion establishment between sister chromatids during mitotic divisions is accompanied by recruitment of the cohesion-stabilizing protein Sororin. During somatic cell division cycles, Sororin is recruited in response to DNA replication-dependent modification of the cohesin complex by ESCO acetyltransferases. How Sororin is recruited and acts in meiosis is less clear. Here, we have surveyed the chromosomal localization of Sororin and its relationship to the meiotic cohesins and other chromatin modifiers with the objective of determining how Sororin contributes to meiotic chromosome dynamics. We show that Sororin localizes to the cores of meiotic chromosomes in a manner that is dependent on synapsis and the synaptonemal complex protein SYCP1. In contrast, cohesin, with which Sororin interacts in mitotic cells, shows axial enrichment on meiotic chromosomes even in the absence of synapsis between homologs. Using high-resolution microscopy, we show that Sororin is localized to the central region of the synaptonemal complex. These results indicate that Sororin regulation during meiosis is distinct from its regulation in mitotic cells and may suggest that it interacts with a distinctly different partner to ensure proper chromosome dynamics in meiosis.
authors.affiliation: Biochemistry and Molecular Biology, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, 21205, USA.
authors.forename: Philip W
authors.initials: PW
authors.lastname: Jordan
authors.affiliation: Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, 825 NE 13th St., Oklahoma City, OK, 73104, USA.
authors.forename: Craig
authors.initials: C
authors.lastname: Eyster
authors.affiliation: Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, 825 NE 13th St., Oklahoma City, OK, 73104, USA.
authors.forename: Jingrong
authors.initials: J
authors.lastname: Chen
authors.affiliation: Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, 825 NE 13th St., Oklahoma City, OK, 73104, USA. Roberto-Pezza@omrf.org.|Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA. Roberto-Pezza@omrf.org.
authors.forename: Roberto J
authors.initials: RJ
authors.lastname: Pezza
authors.affiliation: Cell Cycle and Cancer Biology Program, Oklahoma Medical Research Foundation, 825 NE 13th St., Oklahoma City, OK, 73104, USA. Susannah-Rankin@omrf.org.|Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK, 73104, USA. Susannah-Rankin@omrf.org.
authors.forename: Susannah
authors.identifier: 0000-0002-4056-6610
authors.initials: S
authors.lastname: Rankin
mesh_terms: D048868:Adaptor Proteins, Signal Transducing / Q000235:genetics / Q000378:metabolism*; D000818:Animals; D018797:Cell Cycle Proteins / Q000235:genetics / Q000378:metabolism*; D002868:Chromosomal Proteins, Non-Histone / Q000378:metabolism; D023902:Chromosome Pairing; D002875:Chromosomes / Q000737:chemistry* / Q000378:metabolism / Q000648:ultrastructure; D008540:Meiosis; D051379:Mice; D008938:Mitosis; D013573:Synaptonemal Complex; D000097722:Cohesins
chemical_list: D048868:Adaptor Proteins, Signal Transducing; D018797:Cell Cycle Proteins; D002868:Chromosomal Proteins, Non-Histone; D000097722:Cohesins; C500696:Cdca5 protein, mouse
keywords: Chromosome synapsis; Cohesin; Meiosis; Meiotic prophase; Sororin