title: A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System.
abstract: The structure of a self-assembly formed from a cationic azobenzene derivative, 4-cholesterocarbonyl-4'-(N,N,N-triethylamine butyloxyl bromide) azobenzene (CAB) and surfactant sodium dodecyl sulfate (SDS) in aqueous solution was studied by cryo-TEM and synchrotron radiation small-angle X-ray scattering (SAXS). Both unilamellar and multilamellar vesicles could be observed. CAB in vesicles were capable to undergo reversible trans-to-cis isomerization upon UV or visible light irradiation. The structural change upon UV light irradiation could be catched by SAXS, which demonstrated that the interlamellar spacing of the cis-multilamellar vesicles increased by 0.2-0.3 nm. Based on this microstructural change, the release of rhodamine B (RhB) and doxorubicin (DOX) could be triggered by UV irradiation. When incubated NIH 3T3 cells and Bel 7402 cells with DOX-loaded CAB/SDS vesicles, UV irradiation induced DOX release decreased the viability of both cell lines significantly compared with the non-irradiated cells. The in vitro experiment indicated that CAB/SDS vesicles had high efficiency to deliver loaded molecules into cells. The in vivo experiment showed that CAB/SDS vesicles not only have high drug delivery efficiency into rat retinas, but also could maintain high drug concentration for a longer time. CAB/SDS catanionic vesicles may find potential applications as a smart drug delivery system for controlled release by light.
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.|Photonics Control Technology Team, Advanced Photonics Technology Development Group, Center for Advanced Photonics, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Shengyong
authors.initials: S
authors.lastname: Geng
authors.affiliation: Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201204, China.
authors.forename: Yuzhu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Liping
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Kouyama
authors.affiliation: Department of Physics, Graduate School of Science, Nagoya University, Nagoya 464-8602, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Gotoh
authors.affiliation: Photonics Control Technology Team, Advanced Photonics Technology Development Group, Center for Advanced Photonics, RIKEN, Wako, Saitama 351-0198, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Wada
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.
authors.forename: Jin-Ye
authors.initials: JY
authors.lastname: Wang
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000032:analysis; D001391:Azo Compounds / Q000378:metabolism*; D002460:Cell Line; D020285:Cryoelectron Microscopy; D004317:Doxorubicin / Q000032:analysis; D016503:Drug Delivery Systems; D005456:Fluorescent Dyes / Q000032:analysis; D006801:Humans; D008027:Light; D051379:Mice; D012235:Rhodamines / Q000032:analysis; D053838:Scattering, Small Angle; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism*; D014466:Ultraviolet Rays
chemical_list: D000970:Antineoplastic Agents; D001391:Azo Compounds; D005456:Fluorescent Dyes; D012235:Rhodamines; D012967:Sodium Dodecyl Sulfate; D004317:Doxorubicin; C009850:azobenzene; C029773:rhodamine B