title: A pilot study for construction of a new cadmium-sensing yeast strain carrying a reporter plasmid with the JLP1 promoter.
abstract: Cadmium contamination still occurs in some parts of the world, and its concentrations in the environment are monitored in most countries due to its adverse effects on human health. We herein established yeast (Saccharomyces cerevisiae) reporter assay strains carrying plasmids with the yeast JLP1, SEO1, and CUP1 promoters connected to the bacterial lacZ reporter gene. The strain carrying the high-copy number pESC-JLP1-lacZ reporter plasmid was more responsive to cadmium than strains with other reporter plasmids. This JLP1-lacZ reporter assay strain will be useful for monitoring cadmium contamination in environmental water and soil as a first screening tool preceding official instrumental analyses, because the assay is rapid, easy to handle, and has the ability to process a large number of samples at a low cost.
authors.affiliation: Department of Biology, Graduate School of Science, Osaka Prefecture University.
authors.forename: Sayoko
authors.initials: S
authors.lastname: Ito-Harashima
authors.forename: Yui
authors.initials: Y
authors.lastname: Mizutani
authors.forename: Motoshi
authors.initials: M
authors.lastname: Nishimura
authors.forename: Hyo Jeong
authors.initials: HJ
authors.lastname: Kim
authors.forename: Yeo Jin
authors.initials: YJ
authors.lastname: Kim
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.forename: Ji Hye
authors.initials: JH
authors.lastname: Bae
authors.forename: Preeyaporn
authors.initials: P
authors.lastname: Koedrith
authors.forename: Masanobu
authors.initials: M
authors.lastname: Kawanishi
authors.forename: Young Rok
authors.initials: YR
authors.lastname: Seo
authors.forename: Takashi
authors.initials: T
authors.lastname: Yagi
mesh_terms: D002104:Cadmium / Q000633:toxicity*; D049308:Dioxygenases / Q000235:genetics*; D004785:Environmental Pollutants / Q000633:toxicity*; D004926:Escherichia coli / Q000235:genetics; D005800:Genes, Fungal; D017930:Genes, Reporter; D007763:Lac Operon; D010865:Pilot Projects; D010957:Plasmids; D011401:Promoter Regions, Genetic; D012441:Saccharomyces cerevisiae / Q000187:drug effects* / Q000235:genetics* / Q000254:growth & development; D029701:Saccharomyces cerevisiae Proteins / Q000235:genetics*
chemical_list: D004785:Environmental Pollutants; D029701:Saccharomyces cerevisiae Proteins; D002104:Cadmium; D049308:Dioxygenases; C121605:JLP1 protein, S cerevisiae