title: Paraspeckles as rhythmic nuclear mRNA anchorages responsible for circadian gene expression.
abstract: Circadian clocks regulate rhythmic gene expression levels by means of mRNA oscillations that are mainly driven by post-transcriptional regulation. We identified a new post-transcriptional mechanism, which involves nuclear bodies called paraspeckles. Major components of paraspeckles including the long noncoding RNA Neat1, which is the structural component, and its major protein partners, as well as the number of paraspeckles, follow a circadian pattern in pituitary cells. Paraspeckles are known to retain within the nucleus RNAs containing inverted repeats of Alu sequences. We showed that a reporter gene in which these RNA duplex elements were inserted in the 3'-UTR region displayed a circadian expression. Moreover, circadian endogenous mRNA associated with paraspeckles lost their circadian pattern when paraspeckles were disrupted. This work not only highlights a new paraspeckle-based post-transcriptional mechanism involved in circadian gene expression but also provides the list of all mRNA associated with paraspeckles in the nucleus of pituitary cells.
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Manon
authors.initials: M
authors.lastname: Torres
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Denis
authors.initials: D
authors.lastname: Becquet
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Marie-Pierre
authors.initials: MP
authors.lastname: Blanchard
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Guillen
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Bénédicte
authors.initials: B
authors.lastname: Boyer
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Mathias
authors.initials: M
authors.lastname: Moreno
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Franc
authors.affiliation: a Aix Marseille Université, CNRS, CRN2M-UMR7286, Faculté de Médecine Nord , Marseille , France.
authors.forename: Anne-Marie
authors.identifier: 0000-0002-3278-4642
authors.initials: AM
authors.lastname: François-Bellan
mesh_terms: D000818:Animals; D002467:Cell Nucleus / Q000378:metabolism*; D002940:Circadian Rhythm / Q000235:genetics*; D005786:Gene Expression Regulation; D010902:Pituitary Gland / Q000166:cytology; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism
chemical_list: D012333:RNA, Messenger
keywords: circadian oscillators; circadian rhythm; nuclear RNA retention; paraspeckle; post-transcriptional mechanism