title: Molecular characterization of a p38 mitogen-activated protein kinase gene from Scylla paramamosain and its expression profiles during pathogenic challenge.
abstract: A novel p38 MAPK gene from S. paramamosain was cloned and characterized by rapid amplification of cDNA ends (RACE) technology. S. paramamosain p38 (Sp-p38) MAPK gene consists of an open reading frame of 1095bp encoding a 365-amino-acid protein, which showed close phylogenetic relationship to Litopenaeus vannamei p38 MAPK. The tissue distribution patterns showed that Sp-p38 MAPK was widely expressed in all examined tissues, with the highest expression in hemocytes and intestines. The expression levels of Sp-p38 MAPK in hemocytes was up-regulated post-stimulation, which reached the peak at 6h and 12h after bacteria (S. aureus and V. harveyi) and WSSV infection, respectively. In conclusion, our data contributed to define the biological characteristics of Sp-p38 MAPK and further demonstrated the critical role of Sp-p38 MAPK in vivo during the viral and bacterial infection.
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
authors.forename: Zehui
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China. Electronic address: gengyisicau@126.com.
authors.forename: Yi
authors.initials: Y
authors.lastname: Geng
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
authors.forename: Anming
authors.initials: A
authors.lastname: Huang
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
authors.forename: Kaiyu
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Huang
authors.affiliation: Department of Aquaculture, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
authors.forename: Defang
authors.initials: D
authors.lastname: Chen
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
authors.forename: Yangping
authors.initials: Y
authors.lastname: Ou
authors.affiliation: College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Sichuan 625014, PR China.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D060829:Arthropod Proteins / Q000235:genetics*; D001483:Base Sequence; D003386:Brachyura / Q000201:enzymology* / Q000235:genetics*; D020869:Gene Expression Profiling; D010802:Phylogeny; D016133:Polymerase Chain Reaction; D059467:Transcriptome; D048051:p38 Mitogen-Activated Protein Kinases / Q000235:genetics*
chemical_list: D060829:Arthropod Proteins; D048051:p38 Mitogen-Activated Protein Kinases
keywords: Cloning; Expression profiles; P38 mitogen-activated protein kinase gene; Pathogen infection; Scylla paramamosain