title: The effect of arginine on the Wnt/β-catenin signaling pathway during porcine intramuscular preadipocyte differentiation.
abstract: Dietary l-arginine supplementation decreases backfat thickness and increases intramuscular fat content in growing-finishing pigs, but the underlying mechanisms are unknown. In this study, the effect of arginine on differentiation of porcine intramuscular preadipocytes was investigated in vitro. We showed that the mRNA and protein expressions of the peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer binding protein α were upregulated by arginine supplementation. Furthermore, the intracellular triglyceride level was increased by arginine supplementation. We also showed that activation of the Wnt/β-catenin signal pathway by using lithium chloride (LiCl) significantly attenuated arginine-induced upregulation of PPARγ and increased the phospho-β-catenin level. These findings suggested that arginine promotes porcine intramuscular preadipocyte differentiation, which might be via repressing the Wnt/β-catenin signaling pathway.
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China. zqhuang@sicau.edu.cn.
authors.forename: Xiaoling
authors.initials: X
authors.lastname: Chen
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China. zqhuang@sicau.edu.cn.
authors.forename: Yanliu
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China. zqhuang@sicau.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Jia
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China. zqhuang@sicau.edu.cn.
authors.forename: Guangmang
authors.initials: G
authors.lastname: Liu
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China. zqhuang@sicau.edu.cn.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 611130, P. R. China. zqhuang@sicau.edu.cn.
authors.forename: Zhiqing
authors.initials: Z
authors.lastname: Huang
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000378:metabolism*; D050156:Adipogenesis; D000818:Animals; D001120:Arginine / Q000378:metabolism*; D022763:CCAAT-Enhancer-Binding Protein-alpha / Q000235:genetics / Q000378:metabolism*; D002478:Cells, Cultured; D005260:Female; D008297:Male; D047495:PPAR gamma / Q000235:genetics / Q000378:metabolism*; D013552:Swine; D014280:Triglycerides / Q000378:metabolism; D060449:Wnt Signaling Pathway
chemical_list: D022763:CCAAT-Enhancer-Binding Protein-alpha; D047495:PPAR gamma; D014280:Triglycerides; D001120:Arginine