title: Omics for understanding synergistic action of validamycin A and Trichoderma asperellum GDFS1009 against maize sheath blight pathogen.
abstract: Sheath blight, causes by Rhizoctonia spp., threaten maize yield every year throughout the world. Trichoderma could degrade Rhizoctonia solani on maize mainly via competition and hyperparasitism, whereas validamycin A could efficiently inhibit the growth of R. solani via disturbing the energy system. By contrast, validamycin A is efficient but it takes effect in a short period, while Trichoderma takes effect in a long period though time-consuming. To overcome the disadvantages, Trichoderma asperellum GDFS1009 was used together with validamycin A. In vitro tests proved that the combined pathogen-inhibiting efficiency was significantly improved. Furthermore, results based on transcriptome and metabolome showed that validamycin A had no significant effects on growth, basic metabolism and main bio-control mechanisms of T. asperellum GDFS1009. Such few impacts may be attributed to detoxification and tolerance mechanism of T. asperellum GDFS1009. In addition, T. asperellum GDFS1009 has an ability to relieve the stress caused by validaymicn A. Meanwhile, liquid chromatography-mass spectrometry (LC-MS) results showed that only minor degradation (20%) of validamycin A was caused by T. asperellum GDFS1009 during cofermentation. All results together provide solid bases for validamycin A synergy with T. asperellum GDFS1009 in their combined biocontrol application.
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Qiong
authors.initials: Q
authors.lastname: Wu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Lida
authors.initials: L
authors.lastname: Zhang
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Hai
authors.initials: H
authors.lastname: Xia
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Chuanjin
authors.initials: C
authors.lastname: Yu
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Kai
authors.initials: K
authors.lastname: Dou
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.
authors.forename: Yaqian
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Agriculture and Biology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China.|State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P.R. China.|The Key laboratory of Urban (South) Agriculture, Ministry of Agriculture, Shanghai 200240, P.R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Chen
mesh_terms: D000900:Anti-Bacterial Agents / Q000008:administration & dosage*; D007294:Inositol / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000378:metabolism; D010935:Plant Diseases / Q000517:prevention & control*; D012232:Rhizoctonia / Q000187:drug effects / Q000472:pathogenicity*; D014242:Trichoderma / Q000378:metabolism*; D003313:Zea mays / Q000187:drug effects* / Q000378:metabolism / Q000469:parasitology
chemical_list: D000900:Anti-Bacterial Agents; C030790:validamycin A; D007294:Inositol