title: Production of the Non-apoptotic Metalloprotease-Cleaved CD95L and Its Cytotoxic Recombinant Counterpart Designed Ig-CD95L.
abstract: The ligand of CD95, CD95L (also known as FasL or CD178), is a type II transmembrane protein that belongs to the Tumor Necrosis factor (TNF) family (Fig. 1a). This membrane-bound cytokine is mainly expressed at the surface of activated T lymphocytes and natural killer cells, where it is used as an apoptotic factor to eliminate infected and transformed cells (Strasser et al., Immunity 30:180-192, 2009).
authors.affiliation: Centre Eugène Marquis, rue Bataille Flandres Dunkerque, 35042, Rennes, France. eleonore.lepvrier@gmail.com.|INSERM ERL440-OSS, Equipe Labellisée Ligue Contre Le Cancer, Rennes, France. eleonore.lepvrier@gmail.com.|Université de Rennes-1, 2 av. du Prof. Léon Bernard, 35043, Rennes, France. eleonore.lepvrier@gmail.com.
authors.forename: Eléonore
authors.initials: E
authors.lastname: Lepvrier
authors.affiliation: Centre Eugène Marquis, rue Bataille Flandres Dunkerque, 35042, Rennes, France.|INSERM ERL440-OSS, Equipe Labellisée Ligue Contre Le Cancer, Rennes, France.|Université de Rennes-1, 2 av. du Prof. Léon Bernard, 35043, Rennes, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Martin
authors.affiliation: Centre Eugène Marquis, rue Bataille Flandres Dunkerque, 35042, Rennes, France. b.collet@rennes.unicancer.fr.|INSERM ERL440-OSS, Equipe Labellisée Ligue Contre Le Cancer, Rennes, France. b.collet@rennes.unicancer.fr.|Université de Rennes-1, 2 av. du Prof. Léon Bernard, 35043, Rennes, France. b.collet@rennes.unicancer.fr.
authors.forename: Brigitte
authors.initials: B
authors.lastname: Collet
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D004797:Enzyme-Linked Immunosorbent Assay; D053222:Fas Ligand Protein / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics; D015870:Gene Expression; D057809:HEK293 Cells; D006801:Humans; D045726:Metalloproteases / Q000378:metabolism*; D059748:Proteolysis; D011993:Recombinant Fusion Proteins / Q000096:biosynthesis* / Q000235:genetics / Q000494:pharmacology; D015398:Signal Transduction; D014162:Transfection
chemical_list: D053222:Fas Ligand Protein; D011993:Recombinant Fusion Proteins; D045726:Metalloproteases
keywords: CD95; CD95L; FasL; Tumor Necrosis factor