title: Utility of urinary tubular markers for monitoring chronic tubulointerstitial injury after ischemia-reperfusion.
abstract: OBJECTIVE
  The aim of this study was to elucidate whether urinary tubular markers during the chronic phase of acute kidney injury (AKI) are associated with chronic tubulointerstitial damage.
  
  
  METHODS
  Male human L-type fatty acid binding protein (L-FABP) chromosomal transgenic (Tg) mice underwent ischaemic reperfusion (I/R) injury via renal pedicle clamping for either 10 min or 20 min. Contralateral nephrectomy was performed at the time of tissue reperfusion. The kidneys were analyzed 20 days after the last I/R.
  
  
  RESULTS
  Serum creatinine levels 20 days post-I/R were significantly higher in the 20 min I/R than in the 10 min I/R and control groups and were similar between the 10 min I/R and control groups. The degree of tubulointerstitial damage 20 days post-I/R was significantly more severe in the 20 min I/R than in the 10 min I/R and control groups, as well as in the 10 min I/R than in the control group. Urinary levels of human L-FABP, albumin, and kidney injury molecule-1 (KIM-1) 20 days post-I/R were significantly higher in the 20 min I/R than in the control group, whereas urinary L-FABP was significantly higher in the 10 min I/R than in the control group. Conversely, urinary neutrophil gelatinase-associated lipocalin levels did not significantly differ between the three groups. Finally, the urinary levels of human L-FABP, albumin, and KIM-1 levels 20 days post-I/R were significantly correlated with the degree of renal damage.
  
  
  CONCLUSIONS
  Urinary levels of human L-FABP, albumin and, KIM-1 may be useful for monitoring AKI-to-CKD transition in clinical practice.
authors.affiliation: Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Ichikawa
authors.affiliation: Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.|Department of Anatomy, St. Marianna University School of Medicine, Kanagawa, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Kamijo-Ikemori
authors.affiliation: Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.
authors.forename: Takeshi
authors.initials: T
authors.lastname: Sugaya
authors.affiliation: Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.
authors.forename: Keiichi
authors.initials: K
authors.lastname: Ohata
authors.affiliation: Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.
authors.forename: Mikako
authors.initials: M
authors.lastname: Hisamichi
authors.affiliation: Department of Anatomy, St. Marianna University School of Medicine, Kanagawa, Japan.
authors.forename: Seiko
authors.initials: S
authors.lastname: Hoshino
authors.affiliation: JCHO Tokyo Takanawa Hospital, Tokyo, Japan.
authors.forename: Kenjiro
authors.initials: K
authors.lastname: Kimura
authors.affiliation: Division of Nephrology and Hypertension, Department of Internal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.
authors.forename: Yugo
authors.initials: Y
authors.lastname: Shibagaki
mesh_terms: D058186:Acute Kidney Injury / Q000473:pathology / Q000652:urine*; D000419:Albuminuria / Q000473:pathology / Q000652:urine*; D000818:Animals; D015415:Biomarkers / Q000097:blood / Q000652:urine; D003404:Creatinine / Q000097:blood; D004195:Disease Models, Animal; D050556:Fatty Acid-Binding Proteins / Q000235:genetics / Q000652:urine*; D005355:Fibrosis; D000072596:Hepatitis A Virus Cellular Receptor 1 / Q000378:metabolism*; D007684:Kidney Tubules / Q000378:metabolism* / Q000473:pathology; D008297:Male; D008807:Mice, Inbred BALB C; D008808:Mice, Inbred CBA; D008822:Mice, Transgenic; D011237:Predictive Value of Tests; D015427:Reperfusion Injury / Q000473:pathology / Q000652:urine*; D012720:Severity of Illness Index; D013997:Time Factors; D016482:Urinalysis
chemical_list: D015415:Biomarkers; C493319:FABP1 protein, human; D050556:Fatty Acid-Binding Proteins; C442240:Havcr1 protein, mouse; D000072596:Hepatitis A Virus Cellular Receptor 1; D003404:Creatinine
keywords: L-type fatty acid binding protein; albumin; ischaemia-reperfusion; kidney injury molecule-1; urinary marker