title: Phosphorylated Radix Cyathulae officinalis Polysaccharides Act as Adjuvant via Promoting Dendritic Cell Maturation.
abstract: The aim of this study was to investigate whether phosphorylatedRadix Cyathulae officinalis Kuanpolysaccharides (pRCPS) used as adjuvant with foot-and-mouth disease vaccine (FMDV) can stimulate specific humoral and cellular immune responses in ICR mice. The results demonstrated that pRCPS significantly up-regulated FMDV-specific IgG, IgG1, IgG2b and IgG2a antibody levels and splenocyte proliferation. pRCPS also promoted the killing activities of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). In addition, pRCPS enhanced the expression levels of IL-2, IL-4, and IFN-γ in CD4⁺ T cells and the level of IFN-γ in CD8⁺ T cells. Importantly, pRCPS enhanced the expression of MHCII, CD40⁺, CD86⁺, and CD80⁺ in dendritic cells (DCs). This study indicated that phosphorylation modification could increase immune-enhancing activities of RCPS, and pRCPS could promote humoral and cellular immune responses through facilitating DC maturation.
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China. fenghaiborc@126.com.|College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA. fenghaiborc@126.com.
authors.forename: Haibo
authors.initials: H
authors.lastname: Feng
authors.affiliation: College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA. spm13@cornell.edu.
authors.forename: Sean P
authors.initials: SP
authors.lastname: McDonough
authors.affiliation: Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610051, China. fanjingcdu@sina.com.
authors.forename: Jing
authors.initials: J
authors.lastname: Fan
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China. spyswu@sina.com.
authors.forename: Shiping
authors.initials: S
authors.lastname: Yang
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China. fenghb1981@126.com.
authors.forename: Xuelian
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China. luyongswu@163.com.
authors.forename: Yong
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China. umbrella_fj@163.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Department of Veterinary Medicine, Southwest University, Rongchang, Chongqing 402460, China. yixiaoswu@163.com.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yi
authors.affiliation: College of Veterinary Medicine, Cornell University, Ithaca, NY 14850, USA. yc42@cornell.edu.
authors.forename: Yung-Fu
authors.initials: YF
authors.lastname: Chang
mesh_terms: D000276:Adjuvants, Immunologic / Q000627:therapeutic use; D000818:Animals; D002454:Cell Differentiation / Q000276:immunology; D016207:Cytokines / Q000276:immunology; D003713:Dendritic Cells / Q000276:immunology*; D005536:Foot-and-Mouth Disease / Q000276:immunology* / Q000517:prevention & control; D005786:Gene Expression Regulation / Q000276:immunology; D006801:Humans; D007111:Immunity, Cellular; D000071497:Immunogenicity, Vaccine / Q000276:immunology; D007376:Interleukin-2 / Q000276:immunology; D015847:Interleukin-4 / Q000276:immunology; D007694:Killer Cells, Natural / Q000276:immunology; D051379:Mice; D010587:Phagocytosis / Q000187:drug effects / Q000276:immunology*; D010766:Phosphorylation / Q000276:immunology; D018517:Plant Roots / Q000737:chemistry / Q000276:immunology; D011134:Polysaccharides / Q000737:chemistry / Q000276:immunology / Q000627:therapeutic use*; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology
chemical_list: D000276:Adjuvants, Immunologic; D016207:Cytokines; D007376:Interleukin-2; D011134:Polysaccharides; D015847:Interleukin-4
keywords: Radix Cyathulae polysaccharide; adjuvant; dendritic cell; phosphorylation modification