title: Recent advancements in lateral flow immunoassays: A journey for toxin detection in food.
abstract: Biotechnology embraces various physical and chemical phenomena toward advancement of health diagnostics. Toward such advancement, detection of toxins plays an important role. Toxins produce severe health impacts on consumption with high mortality associated in acute cases. The most prominent route of infection and intoxication is through food matrices. Therefore, rapid detection of toxins at low concentrations is the need of modern diagnostics. Lateral flow immunoassays are one of the emergent and popularly used rapid detection technology developed for detecting various kinds of analytes. This review thus focuses on recent advancements in lateral flow immunoassays for detecting different toxins in agricultural food. Appropriate emphasis was given on how the labels, recognition elements, or detection strategy has laid an impact on improvement in immunochromatographic assays for toxins. The paper also discusses the gradual change in sensitivities and specificities of assays in accordance with the method of food processing used. The review concludes with the major challenges faced by this technology and provides an outlook and insight of ideas to improve it in the future.
authors.affiliation: a Department of Biotechnology , Motilal Nehru National Institute of Technology , Allahabad , Uttar Pradesh , India.
authors.forename: Pranav
authors.initials: P
authors.lastname: Tripathi
authors.affiliation: a Department of Biotechnology , Motilal Nehru National Institute of Technology , Allahabad , Uttar Pradesh , India.
authors.forename: Neha
authors.initials: N
authors.lastname: Upadhyay
authors.affiliation: a Department of Biotechnology , Motilal Nehru National Institute of Technology , Allahabad , Uttar Pradesh , India.
authors.forename: Seema
authors.initials: S
authors.lastname: Nara
mesh_terms: D001709:Biotechnology; D005506:Food Contamination / Q000032:analysis*; D007118:Immunoassay / Q000379:methods*; D014118:Toxins, Biological / Q000737:chemistry*
chemical_list: D014118:Toxins, Biological
keywords: Diagnostics; LOD; commercial LFIA; immunoassay; toxins