title: Road to the future of systems biotechnology: CRISPR-Cas-mediated metabolic engineering for recombinant protein production.
abstract: The rising potential for CRISPR-Cas-mediated genome editing has revolutionized our strategies in basic and practical bioengineering research. It provides a predictable and precise method for genome modification in a robust and reproducible fashion. Emergence of systems biotechnology and synthetic biology approaches coupled with CRISPR-Cas technology could change the future of cell factories to possess some new features which have not been found naturally. We have discussed the possibility and versatile potentials of CRISPR-Cas technology for metabolic engineering of a recombinant host for heterologous protein production. We describe the mechanisms involved in this metabolic engineering approach and present the diverse features of its application in biotechnology and protein production.
authors.affiliation: a Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies , Shiraz , Iran.|c Department of Medical Biotechnology, School of Medicine , Fasa University of Medical Sciences , Fasa , Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Roointan
authors.affiliation: a Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies , Shiraz , Iran.|b Pharmaceutical Sciences Research Center, School of Pharmacy , Shiraz University of Medical Sciences , Shiraz , Iran.
authors.forename: Mohammad Hossein
authors.initials: MH
authors.lastname: Morowvat
mesh_terms: D064113:CRISPR-Cas Systems; D000072669:Gene Editing; D060847:Metabolic Engineering / Q000379:methods*; D011994:Recombinant Proteins / Q000378:metabolism; D058615:Synthetic Biology
chemical_list: D011994:Recombinant Proteins
keywords: CRISPR–Cas; genome editing; metabolic engineering; recombinant protein production; synthetic biology; systems biotechnology