title: Alkaline Phosphatase-Triggered Simultaneous Hydrogelation and Chemiluminescence.
abstract: Chemiluminescence (CL) has a higher signal-to-noise ratio than fluorescence, but the use of CL to track an enzyme-instructed self-assembly (EISA) process has not been reported. In this work, by coincubation of the hydrogelator precursor Fmoc-Phe-Phe-Tyr(H2PO3)-OH (1P) and the CL agent AMPPD (2) with alkaline phosphatase (ALP), we employed CL to directly characterize and image the simultaneous EISA process of 1P. Hydrogelation processes of 1P with and without 2 and the CL properties of 2 with and without 1P under ALP catalysis were systematically studied. The results indicated that 2 is an ideal CL indicator for ALP-triggered hydrogelation of 1P. Using an IVIS optical imaging system, we obtained time-course CL images of 2 to track the simultaneous hydrogelation process of 1P in the same solution. We envision that our CL method could be employed to track more biological EISA events in the near future.
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Zijuan
authors.initials: Z
authors.lastname: Hai
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Jindan
authors.initials: J
authors.lastname: Li
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Xu
authors.affiliation: CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
authors.forename: Gaolin
authors.identifier: 0000-0002-6159-9999
authors.initials: G
authors.lastname: Liang
mesh_terms: D000469:Alkaline Phosphatase / Q000737:chemistry / Q000378:metabolism*; D055162:Biocatalysis; D005782:Gels / Q000737:chemistry / Q000378:metabolism; D007700:Kinetics; D049449:Luminescence; D008968:Molecular Conformation; D061848:Optical Imaging; D010316:Particle Size
chemical_list: D005782:Gels; D000469:Alkaline Phosphatase