title: Characterization and Antibacterial Potential of Lactic Acid BacteriumPediococcus pentosaceus4I1 Isolated from Freshwater FishZacco koreanus.
abstract: This study was undertaken to characterize a lactic acid bacterium 4I1, isolated from the freshwater fish,Zacco koreanus. Morphological, biochemical, and molecular characterization of 4I1 revealed it to bePediococcus pentosaceus4I1. The cell free supernatant (CFS) ofP. pentosaceus4I1 exhibited significant (p< 0.05) antibacterial effects (inhibition zone diameters: 16.5-20.4 mm) against tested foodborne pathogenic bacteria with MIC and MBC values of 250-500 and 500-1,000 μg/mL, respectively. Further, antibacterial action of CFS ofP. pentosaceus4I1 against two selected bacteriaStaphylococcus aureusKCTC-1621 andEscherichia coliO157:H7 was determined in subsequent assays. The CFS ofP. pentosaceus4I1 revealed its antibacterial action againstS. aureusKCTC-1621 andE. coliO157:H7 on membrane integrity as confirmed by a reduction in cell viability, increased potassium ion release (900 and 800 mmol/L), reduced absorption at 260-nm (3.99 and 3.77 OD), and increased relative electrical conductivity (9.9 and 9.7%), respectively. Gas chromatography-mass spectrometry (GC-MS) analysis of the CFS ofP. pentosaceus4I1 resulted in the identification of seven major compounds, which included amino acids, fatty acids and organic acids. Scanning electron microscopic-based morphological analysis further confirmed the antibacterial effect of CFS ofP. pentosaceus4I1 againstS. aureusKCTC-1621 andE. coliO157:H7. In addition, the CFS ofP. Pentosaceus4I1 displayed potent inhibitory effects on biofilms formation byS. aureusKCTC-1621 andE. coliO157:H7. The study indicates the CFS ofP. pentosaceus4I1 offers an alternative means of controlling foodborne pathogens.
authors.affiliation: Department of Applied Microbiology and Biotechnology, Yeungnam University Gyeongsan, South Korea.
authors.forename: Vivek K
authors.initials: VK
authors.lastname: Bajpai
authors.affiliation: National Science Museum, Ministry of Science, ICT and Future Planning Daejeon, South Korea.
authors.forename: Jeong-Ho
authors.initials: JH
authors.lastname: Han
authors.affiliation: Department of Applied Microbiology and Biotechnology, Yeungnam University Gyeongsan, South Korea.
authors.forename: Irfan A
authors.initials: IA
authors.lastname: Rather
authors.affiliation: National Science Museum, Ministry of Science, ICT and Future Planning Daejeon, South Korea.
authors.forename: Chanseo
authors.initials: C
authors.lastname: Park
authors.affiliation: National Science Museum, Ministry of Science, ICT and Future Planning Daejeon, South Korea.
authors.forename: Jeongheui
authors.initials: J
authors.lastname: Lim
authors.affiliation: National Science Museum, Ministry of Science, ICT and Future Planning Daejeon, South Korea.
authors.forename: Woon Kee
authors.initials: WK
authors.lastname: Paek
authors.affiliation: Kcellbio Seoul, South Korea.
authors.forename: Jong Sung
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Kcellbio Seoul, South Korea.
authors.forename: Jung-In
authors.initials: JI
authors.lastname: Yoon
authors.affiliation: Department of Applied Microbiology and Biotechnology, Yeungnam University Gyeongsan, South Korea.
authors.forename: Yong-Ha
authors.initials: YH
authors.lastname: Park
keywords: Pediococcus pentosaceus 4I1; Zacco koreanus; antimicrobial action; cell free supernatant; foodborne pathogens