title: Approaches for Controlled Ag+Ion Release: Influence of Surface Topography, Roughness, and Bactericide Content.
abstract: Silver is the most famous bactericidal element known from ancient times. Its antibacterial and antifungal effects are typically associated with the Ag ionization and concentration of Ag+ions in a bacterial culture. Herein we thoroughly studied the influence of surface topography and roughness on the rate of Ag+ion release. We considered two types of biocompatible and bioactive TiCaPCON-Ag films with 1 and 2 at. % of Ag and nine types of Ti surfaces with an average roughness varying in the range from 5.4 × 10-2to 12.6 μm and different topographic features obtained through polishing, sandblasting, laser treatment, and pulsed electrospark deposition. It is demonstrated that the Ag+ion release rates do not depend on the Ag content in the films as the main parameter, and it is other factors, such as the state of Ag agglomeration, surface topography and roughness, as well as kinetics of surface oxidation, that play a critical role. The obtained results clearly show a synergistic effect of the Ag content in the film and surface topography and roughness on Ag+ion release. By changing the surface topographical features at a constant content of bactericidal element, we showed that the Ag+ion release can be either accelerated by 2.5 times or almost completely suppressed. Despite low Ag+ion concentration in physiological solution (<40 ppb), samples with specially fabricated surface reliefs (flakes or holes) showed a pronounced antibacterial effect already after 3 h of immersion in E. coli bacterial culture. Thus, our results open up new possibilities for the production of cost-effective, scalable, and biologically safe implants with pronounced antibacterial characteristics for future applications in the orthopedic field.
authors.affiliation: National University of Science and Technology "MISIS" , Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: I V
authors.identifier: http://orcid.org/0000-0003-2458-164X
authors.initials: IV
authors.lastname: Sukhorukova
authors.affiliation: National University of Science and Technology "MISIS" , Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: A N
authors.initials: AN
authors.lastname: Sheveyko
authors.affiliation: National University of Science and Technology "MISIS" , Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Shvindina
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology , Obolensk, Moscow Region 142279, Russia.
authors.forename: E A
authors.initials: EA
authors.lastname: Denisenko
authors.affiliation: State Research Center for Applied Microbiology and Biotechnology , Obolensk, Moscow Region 142279, Russia.|Moscow State University , Department of Geocryology, Moscow 119992, Russia.
authors.forename: S G
authors.initials: SG
authors.lastname: Ignatov
authors.affiliation: National University of Science and Technology "MISIS" , Leninsky prospect 4, Moscow 119049, Russia.
authors.forename: D V
authors.identifier: http://orcid.org/0000-0001-7304-2461
authors.initials: DV
authors.lastname: Shtansky
keywords: antibacterial activity; electrochemical behavior; multicomponent films; roughness; silver ion release; surface topography