title: S100A4 (metastasin) positive mesenchymal canine mammary tumour spheroids reduce Tenascin C synthesis under DMSO exposure in vitro.
abstract: In breast cancer research S100A4-positive tumour-associated stromal cells are assumed as primary source of Tenascin C (TNC) in the metastatic environment. Aim of the present study was to isolate and characterize S100A4/TNC positive stromal canine mammary tumour (CMT) cells. Cells grown as scaffold-free spheroids were investigated for S100A4, TNC, and proliferative activity under 1.8% DMSO stimulation by means of Western blot and immunohistochemistry. DMSO is a commonly used drug solvent despite well-known side effects on cells including TNC expression. DMSO did not affect proliferation, but TNC was significantly reduced under DMSO exposure for 7 and 14 days, whereby for S100A4 a reducing effect was only observed after 14 days. Without DMSO, cells stably expressed TNC and S100A4 which makes them suitable to be used in experimental approaches requiring S100A4/TNC expressing CMT stromal cells. Results show that 1.8% DMSO should not be used as solvent for experiments concerning TNC/S100A4 expression.
authors.affiliation: Institute of Anatomy, Histology and Embryology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: S
authors.initials: S
authors.lastname: Kau
authors.affiliation: Institute for Medical Biochemistry, Department for Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria.
authors.forename: I
authors.initials: I
authors.lastname: Miller
authors.affiliation: Platform Biostatistics, Department of Biomedical Science, Institute of Population Genetics, University of Veterinary Medicine, Vienna, Austria.
authors.forename: A
authors.initials: A
authors.lastname: Tichy
authors.affiliation: Institute of Anatomy, Histology and Embryology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria.
authors.forename: C
authors.initials: C
authors.lastname: Gabriel
mesh_terms: D000818:Animals; D015153:Blotting, Western / Q000662:veterinary; D004121:Dimethyl Sulfoxide / Q000494:pharmacology*; D004283:Dog Diseases / Q000378:metabolism*; D004285:Dogs; D005260:Female; D015674:Mammary Neoplasms, Animal / Q000378:metabolism*; D000071999:S100 Calcium-Binding Protein A4 / Q000378:metabolism*; D018874:Spheroids, Cellular / Q000187:drug effects / Q000378:metabolism; D019063:Tenascin / Q000378:metabolism*; D014407:Tumor Cells, Cultured / Q000187:drug effects / Q000378:metabolism
chemical_list: D000071999:S100 Calcium-Binding Protein A4; D019063:Tenascin; D004121:Dimethyl Sulfoxide
keywords: DMSO; S100A4; TNC; canine mammary tumour; tumour stroma