title: Long-term exposure to a 'safe' dose of bisphenol A reduced protein acetylation in adult rat testes.
abstract: Bisphenol A (BPA), a typical environmental endocrine-disrupting chemical, induces epigenetic inheritance. Whether histone acetylation plays a role in these effects of BPA is largely unknown. Here, we investigated histone acetylation in male rats after long-term exposure to a 'safe' dose of BPA. Twenty adult male rats received either BPA (50 μg/kg·bw/day) or a vehicle diet for 35 weeks. Decreased protein lysine-acetylation levels at approximately ~17 kDa and ~25 kDa, as well as decreased histone acetylation of H3K9, H3K27 and H4K12, were detected by Western blot analysis of testes from the treated rats compared with controls. Additionally, increased protein expression of deacetylase Sirt1 and reduced binding of Sirt1, together with increased binding of estrogen receptor β (ERβ) to caveolin-1 (Cav-1), a structural protein component of caveolar membranes, were detected in treated rats compared with controls. Moreover, decreased acetylation of Cav-1 was observed in the treated rats for the first time. Our study showed that long-term exposure to a 'safe' dose of BPA reduces histone acetylation in the male reproductive system, which may be related to the phenotypic paternal-to-offspring transmission observed in our previous study. The evidence also suggested that these epigenetic effects may be meditated by Sirt1 via competition with ERβ for binding to Cav-1.
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.|MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science &Technology, 13 Hangkong Road, Wuhan, 430030, PR China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Clinical Nutrition, Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, PR China.
authors.forename: Xuezhi
authors.initials: X
authors.lastname: Zuo
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Dongliang
authors.initials: D
authors.lastname: He
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Shibin
authors.initials: S
authors.lastname: Ding
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Fangyi
authors.initials: F
authors.lastname: Xu
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Huiqin
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Xin
authors.initials: X
authors.lastname: Jin
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Fan
authors.affiliation: School of Stomatology, Wenzhou Medical University, Wenzhou 325003, PR China.
authors.forename: Li
authors.initials: L
authors.lastname: Ying
authors.affiliation: School of Nursing, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.
authors.forename: Chong
authors.initials: C
authors.lastname: Tian
authors.affiliation: Department of Nutrition and Food Hygiene, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, 13 Hangkong Road, Wuhan 430030, PR China.|MOE Key Lab of Environment and Health, School of Public Health, Tongji Medical College, Huazhong University of Science &Technology, 13 Hangkong Road, Wuhan, 430030, PR China.
authors.forename: Chenjiang
authors.initials: C
authors.lastname: Ying
mesh_terms: D000107:Acetylation / Q000187:drug effects; D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D051242:Caveolin 1 / Q000378:metabolism; D047629:Estrogen Receptor beta / Q000378:metabolism; D008239:Lysine / Q000378:metabolism; D008297:Male; D010636:Phenols / Q000633:toxicity*; D011485:Protein Binding / Q000187:drug effects; D011506:Proteins / Q000378:metabolism*; D017208:Rats, Wistar; D056564:Sirtuin 1 / Q000378:metabolism; D013737:Testis / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology*; D013997:Time Factors; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D051242:Caveolin 1; D047629:Estrogen Receptor beta; D008239:Lysine; D010636:Phenols; D011506:Proteins; D056564:Sirtuin 1; C006780:bisphenol A; D000098826:Bisphenol A Compounds