title: Electrochemical sensor based on magnetic molecularly imprinted nanoparticles modified magnetic electrode for determination of Hb.
abstract: A fast and selective electrochemical sensor for determination of hemoglobin (Hb) was developed based on magnetic molecularly imprinted nanoparticles modified on the magnetic glassy carbon electrode. The nanoparticles Fe3O4@SiO2with a magnetic core and a molecularly imprinted shell had regular structures and good monodispersity. Hb could be determined directly by electrochemical oxidization with the modified electrode. A magnetic field increased electrochemical response to Hb by two times. Imprinting Hb on the surface of Fe3O4@SiO2shortened the response time within 7min. Under optimum conditions, the imprinting factor toward the non-imprinted sensor was 2.8, and the separation factor of Hb to horseradish peroxidase was 2.6. The oxidation peak current had a linear relationship with Hb concentration ranged from 0.005mg/ml to 0.1mg/ml with a detection limit (S/N =3) of 0.0010mg/ml. The sensors were successfully applied to analysis of Hb in whole blood samples with recoveries between 95.7% and 105%.
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Binghua
authors.initials: B
authors.lastname: Sun
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Xinjiong
authors.initials: X
authors.lastname: Ni
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China. Electronic address: yuhuacao64@gmail.com.
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Cao
authors.affiliation: The Key Laboratory of Food Colloids and Biotechnology, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China.
authors.forename: Guangqun
authors.initials: G
authors.lastname: Cao
mesh_terms: D015374:Biosensing Techniques / Q000295:instrumentation; D002244:Carbon / Q000737:chemistry; D055664:Electrochemical Techniques / Q000295:instrumentation*; D004566:Electrodes; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D057230:Limit of Detection; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D054802:Molecular Imprinting; D011108:Polymers / Q000737:chemistry; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D006454:Hemoglobins; D058185:Magnetite Nanoparticles; D011108:Polymers; D002244:Carbon; D012822:Silicon Dioxide
keywords: Fe(3)O(4)@SiO(2); Hb; Magnetic electrode; Magnetic molecularly imprinted nanoparticles