title: Achyranthes japonicaNakai Water Extract Suppresses Binding of IgE Antibody to Cell Surface FcɛRI.
abstract: Achyranthes japonicaNakai (AJN) water extract has a variety of physiological properties, including anti-diabetic, anti-cancer, anti-inflammatory, anti-microbial, and anti-oxidative activities. In the present study, the inhibitory effects of AJN extract were investigated in high affinity immunoglobulin E receptor (FcɛRI)-mediated KU812F cells activation. AJN extract showed suppressive effects on histamine release and intracellular calcium [Ca2+]ielevation from anti-FcɛRI antibody (CRA-1)-stimulated cells in a dose-dependent manner. Flow cytometric analysis showed that AJN extract treatment caused a dose-dependent decrease in the cell surface FcɛRI expression and the binding between the cell surface FcɛRI and the IgE antibody. Moreover, reverse transcription-polymerase chain reaction analysis showed that levels of the mRNA for the FcɛRI α chain was decreased by treatment with AJN extract. These results indicate that AJN extract may exert anti-allergic effects via the inhibition of calcium influx and histamine release, which occurs as a result from the down-regulation of the binding of IgE antibody to cell surface FcɛRI. This mechanism may occur through FcɛRI expression inhibition.
authors.affiliation: College of Pharmacy, Sunchon National University, Jeonnam 57922, Korea.
authors.forename: Sun Yup
authors.initials: SY
authors.lastname: Shim
authors.affiliation: College of Pharmacy, Sunchon National University, Jeonnam 57922, Korea.
authors.forename: Mina
authors.initials: M
authors.lastname: Lee
authors.affiliation: Department of Oriental Medicine Materials, Dongshin University, Jeonnam 58245, Korea.
authors.forename: Kyung Dong
authors.initials: KD
authors.lastname: Lee
keywords: Achyranthes japonica Nakai; FcɛRI; histamine release; human basophilic KU812F cells; immunoglobulin E