title: Periploca forrestiiSaponin Ameliorates Murine CFA-Induced Arthritis by Suppressing Cytokine Production.
abstract: Periploca forrestiiSchltr. has been used as a Chinese folk medicine due to its versatile pharmacological effects such as promoting wounds and rheumatoid arthritis. However, the antiarthritic activity ofPeriploca forrestiisaponin (PFS) and its active compound Periplocin has still not been demonstrated. Here, we evaluated the antiarthritic effects of PFS in adjuvant-induced arthritis (AIA) rats by intragastric administration at a dose of 50 mg/kg. The anti-inflammatory activities of Periplocin were also examined in LPS-induced AIA splenocytes and synoviocytes. PFS significantly ameliorated joint swelling; inhibited bone erosion in joints; lowered levels of IL-6 and TGF-β1 in AIA rat splenocyte; and reduced joint protein expression levels of phospho-STAT3 and IKKα. Using LPS-induced AIA splenocytes, we demonstrate that Periplocin suppressed the key proinflammatory cytokines levels of IL-6, IFN-γ, TGF-β1, and IL-13 and IL-22 and transcription factor levels of T-bet, GATA3, and C-Jun genes. Periplocin also suppressed LPS-induced cytokine secretion from synoviocytes. Our study highlights the antiarthritic activity of PFS and its derived Periplocin and the underlying mechanisms. These results provide a strong rationale for further testing and validation of the use ofPeriploca forrestiiSchltr. as an alternative modality for the treatment of RA.
authors.affiliation: Biotechnology College, Guilin Medical University, No. 109 North 2nd Huan Cheng Road, Guilin, Guangxi 541004, China.
authors.forename: Yingqin
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Biotechnology College, Guilin Medical University, No. 109 North 2nd Huan Cheng Road, Guilin, Guangxi 541004, China.
authors.forename: Minghui
authors.initials: M
authors.lastname: Li
authors.affiliation: Biotechnology College, Guilin Medical University, No. 109 North 2nd Huan Cheng Road, Guilin, Guangxi 541004, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: He
authors.affiliation: Pharmaceutical College, Guilin Medical University, No. 109 North 2nd Huan Cheng Road, Guilin, Guangxi 541004, China.
authors.forename: Xinping
authors.initials: X
authors.lastname: Yang
authors.affiliation: Pharmaceutical College, Guilin Medical University, No. 109 North 2nd Huan Cheng Road, Guilin, Guangxi 541004, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ruan
authors.affiliation: Pharmaceutical College, Guilin Medical University, No. 109 North 2nd Huan Cheng Road, Guilin, Guangxi 541004, China.
authors.forename: Guangchen
authors.identifier: 0000-0001-8266-6014
authors.initials: G
authors.lastname: Sun
mesh_terms: D000818:Animals; D001169:Arthritis, Experimental / Q000188:drug therapy*; D001172:Arthritis, Rheumatoid / Q000188:drug therapy; D016207:Cytokines / Q000378:metabolism*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology; D005260:Female; D005620:Freund's Adjuvant / Q000494:pharmacology; D051550:I-kappa B Kinase / Q000378:metabolism; D007249:Inflammation; D031109:Periploca / Q000737:chemistry*; D051381:Rats; D017207:Rats, Sprague-Dawley; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D012503:Saponins / Q000494:pharmacology*; D013154:Spleen / Q000378:metabolism; D013582:Synovial Fluid / Q000166:cytology
chemical_list: D016207:Cytokines; D004365:Drugs, Chinese Herbal; D050796:STAT3 Transcription Factor; D012503:Saponins; C494088:Stat3 protein, rat; C502381:periplocin; D005620:Freund's Adjuvant; D051550:I-kappa B Kinase