title: Studying cytokinesis in Drosophila epithelial tissues.
abstract: Epithelial tissue cohesiveness is ensured through cell-cell junctions that maintain both adhesion and mechanical coupling between neighboring cells. During development, epithelial tissues undergo intensive cell proliferation. Cell division, and particularly cytokinesis, is coupled to the formation of new adhesive contacts, thereby preserving tissue integrity and propagating cell polarity. Remarkably, the geometry of the new interfaces is determined by the combined action of the dividing cell and its neighbors. To further understand the interplay between the dividing cell and its neighbors, as well as the role of cell division for tissue morphogenesis, it is important to analyze cytokinesis in vivo. Here we present methods to perform live imaging of cell division in Drosophila epithelial tissues and discuss some aspects of image processing and analysis.
authors.affiliation: Institut Curie, PSL Research University, Paris, France; Sorbonne Universités, Paris, France; Abel Salazar Biomedical Sciences Institute, University of Porto, Porto, Portugal.
authors.forename: D
authors.initials: D
authors.lastname: Pinheiro
authors.affiliation: Institut Curie, PSL Research University, Paris, France; Sorbonne Universités, Paris, France.
authors.forename: Y
authors.initials: Y
authors.lastname: Bellaïche
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D016764:Cell Polarity / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D048749:Cytokinesis / Q000235:genetics*; D004331:Drosophila melanogaster / Q000235:genetics / Q000648:ultrastructure; D004848:Epithelium / Q000648:ultrastructure*; D008967:Molecular Biology / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*; D009024:Morphogenesis / Q000235:genetics
keywords: Confocal time-lapse imaging; Cytokinesis; Epithelial tissues; Mechanics