title: Adapted dexamethasone delivery polyethylene oxide and poly(ɛ-caprolactone) construct promote mesenchymal stem cells chondrogenesis.
abstract: Issues associated with tissue transplantation and shortage of donors has always been a concern, whereas tissue engineering has provided the hopeful opportunities. The aim of this study was to investigate the chondrogenic differentiation potential of mesenchymal stem cells (MSCs) in the presence of embedded Dexamethasone into electrospun Poly(ethyleneoxide) nanofibers composited with Poly(ɛ-caprolactone) nanofibers. Electrospun-fabricated scaffolds were characterized by SEM, tensile, contact angle, release profile, MTT assay, and chonderogenic differentiation of stem cells. Results demonstrated Poly(ɛ-caprolactone) properties improved by composite with a Poly(ethyleneoxide). Overall, stem cells seeded on nanofibers content drug showed highest chondrogenic potential in comparison to the other groups.
authors.affiliation: a Department of Biology, Faculty of Science, East Tehran Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Armaghan
authors.initials: A
authors.lastname: Ghiaee
authors.affiliation: a Department of Biology, Faculty of Science, East Tehran Branch , Islamic Azad University , Tehran , Iran.
authors.forename: Faeze
authors.initials: F
authors.lastname: Pournaqi
authors.affiliation: b Stem Cell Technology Research Center , Tehran , Iran.
authors.forename: Saeid
authors.initials: S
authors.lastname: Vakilian
authors.affiliation: c Department of Animal and Poultry Science , College of Aburaihan, University of Tehran , Pakdasht , Iran.
authors.forename: Abdolah
authors.initials: A
authors.lastname: Mohammadi-Sangcheshmeh
authors.affiliation: d Department of Tissue Engineering and Regenerative Medicine , School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences , Tehran , Iran.
authors.forename: Abdolreza
authors.initials: A
authors.lastname: Ardeshirylajimi
mesh_terms: D000328:Adult; D002454:Cell Differentiation / Q000187:drug effects; D020219:Chondrogenesis / Q000187:drug effects*; D003907:Dexamethasone / Q000737:chemistry* / Q000494:pharmacology*; D004337:Drug Carriers / Q000737:chemistry*; D006801:Humans; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000187:drug effects; D011091:Polyesters / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D004337:Drug Carriers; D011091:Polyesters; C016240:polycaprolactone; D011092:Polyethylene Glycols; D003907:Dexamethasone
keywords: Poly(ethyleneoxide); chondroconductive; chondroinductive; dexamethasone; mesenchymal stem cells; poly(ɛ-caprolactone)