title: Characterization of polysaccharides extracted from Platycodon grandiflorus (Jacq.) A.DC. affecting activation of chicken peritoneal macrophages.
abstract: Polysaccharides were isolated from Platycodon grandiflorus (Jacq.) A.DC. (PG) and the effects of three polysaccharides (PGPS80, PGPS60, PGPSt) on their immunological activities were studied. The structure identification of PGPSs was assessed using physicochemical and spectral methods. Results showed that PGPSt(2.67×105Da) compared to PGPS80(1.01×105Da) and PGPS60(1.12×105Da) has relatively higher average molecular weight(Mw) at the first peak with a narrower molecular weight distribution and all consisted of glucose, mannose, arabinose, galactose, xylose and rhamnose in different mass percentages. PGPS80and PGPStlinked mainly by 1,3-and 1,6-β-d-Galp residues. The immunological efficacy of PGPSs was performed on chicken peritoneal macrophages. Results showed that PGPStsignificantly increased phagocytic rates, proliferation and NO production, stimulated macrophages to produce cytokines, including TNF-α, IL-1β and IL-6 as well as stimulated macrophages to express the maturation markers CD80 and CD86. These findings suggest that PGPStexerted significant immunological activity and might be associated with special characters.
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Pimiao
authors.initials: P
authors.lastname: Zheng
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Wentao
authors.initials: W
authors.lastname: Fan
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Shenghua
authors.initials: S
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Pan
authors.initials: P
authors.lastname: Hao
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China.
authors.forename: Huiyu
authors.initials: H
authors.lastname: Wan
authors.affiliation: Agricultural Bio-pharmaceutical Lab. Qingdao Agricultural University, Qingdao 266000, PR China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Hao
authors.affiliation: Research Center for Animal Disease Control Engineering Shandong Province, Tai'an 271018, PR China. Electronic address: Liujz@sdau.edu.cn.
authors.forename: Jianzhu
authors.initials: J
authors.lastname: Liu
authors.affiliation: College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai'an 271018, PR China. Electronic address: zhaoxn@sdau.edu.cn.
authors.forename: Xiaona
authors.initials: X
authors.lastname: Zhao
mesh_terms: D000818:Animals; D018122:B7-1 Antigen / Q000494:pharmacology; D051940:B7-2 Antigen / Q000494:pharmacology; D049109:Cell Proliferation / Q000187:drug effects; D002645:Chickens; D016207:Cytokines / Q000378:metabolism; D056747:Immunomodulation / Q000187:drug effects; D008262:Macrophage Activation / Q000187:drug effects*; D017737:Macrophages, Peritoneal / Q000166:cytology / Q000187:drug effects* / Q000276:immunology* / Q000378:metabolism; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D009569:Nitric Oxide / Q000096:biosynthesis; D031238:Platycodon / Q000737:chemistry*; D011134:Polysaccharides / Q000302:isolation & purification*
chemical_list: D018122:B7-1 Antigen; D051940:B7-2 Antigen; D016207:Cytokines; D009005:Monosaccharides; D011134:Polysaccharides; D009569:Nitric Oxide
keywords: Immunological activity; Platycodon grandiflorus (Jacq.) A.DC; Polysaccharides