title: Fluorescence turn-on hairpin-probe PCR.
abstract: A new fluorescence turn-on type of PCR monitoring system (Hpro-PCR) using a hairpin probe and a primer having a tag sequence at the 5' end with the fluorescent molecule 2,7-diamino-1,8-naphthyridine derivative (DANP) has been developed. The Hpro-PCR exploited the modulation of the equilibrium states between the DANP-bound hairpin structure and probe-tag duplex, and the PCR progress alternated the equilibrium state, resulting in the change of fluorescent intensity.
authors.affiliation: National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan. ft-sakamot@ndmc.ac.jp.
authors.forename: F
authors.identifier: http://orcid.org/0000-0002-0802-3325
authors.initials: F
authors.lastname: Takei
authors.affiliation: The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047, Japan. nakatani@sanken.osaka-u.ac.jp.
authors.forename: K
authors.initials: K
authors.lastname: Nakatani
mesh_terms: D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D009287:Naphthyridines / Q000737:chemistry*; D016133:Polymerase Chain Reaction
chemical_list: C534173:2,7-diamino-1,8-naphthyridine; D005456:Fluorescent Dyes; D009287:Naphthyridines