title: In Vitro Evaluation of the Apoptosis Function in Human Activated T Cells.
abstract: The apoptosis function can be monitored on human lymphocytes by quantifying the induced-death upon apoptotic stimuli involving either the extrinsic or the intrinsic pathway on in vitro activated T cells. Her, we describe an in vitro assay allowing the monitoring of three different apoptosis pathways: (1) the FAS-induced pathway, (2) the activation-induced cell death (AICD), and (3) the death induced by starvation of the cells, called activated-cell autonomous death (ACAD).
authors.affiliation: INSERM UMR 1163, Laboratory of The Immunogenetics of Pediatric Autoimmune Diseases, Paris, France. aude.magerus-chatinet@institutimagine.org.|Imagine Institute, Paris Descartes-Sorbonne Paris Cité University, 24 Boulevard de Montparnasse, 75015, Paris, France. aude.magerus-chatinet@institutimagine.org.
authors.forename: Aude
authors.initials: A
authors.lastname: Magerus-Chatinet
authors.affiliation: INSERM UMR 1163, Laboratory of The Immunogenetics of Pediatric Autoimmune Diseases, Paris, France.|Imagine Institute, Paris Descartes-Sorbonne Paris Cité University, 24 Boulevard de Montparnasse, 75015, Paris, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Rieux-Laucat
mesh_terms: D017209:Apoptosis; D053222:Fas Ligand Protein / Q000378:metabolism; D005434:Flow Cytometry / Q000379:methods; D006801:Humans; D066298:In Vitro Techniques; D008213:Lymphocyte Activation / Q000276:immunology*; D013601:T-Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D019014:fas Receptor / Q000378:metabolism
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor
keywords: ACAD; AICD; Activated T cells; Apoptosis; FAS