title: ARTP mutation and genome shuffling of ABE fermentation symbiotic system for improvement of butanol production.
abstract: Butanol is an ideal renewable biofuel which possesses superior fuel properties. Previously, butanol-producing symbiotic system TSH06 was isolated in our lab, with microoxygen tolerance ability. To boost butanol yield for large-scale industrial production, TSH06 was used as parental strain and subjected to atmospheric and room temperature plasma (ARTP) and four rounds of genome shuffling (GS). ARTP mutant and GS strain were co-cultured with facultative anaerobic Bacillus cereus TSH2 to form a symbiotic system with microoxygen tolerance, which was then subjected to fermentation. Relative messenger RNA (mRNA) level of key enzyme gene was measured by real-time PCR. The highest butanol titer of TS4-30 reached 15.63 g/L, which was 34% higher than TSH06 (12.19 g/L). Compared with parental strain, mRNA of acid-forming gene in TS4-30 decreased in acidogenesis phase, while solvent-forming gene increased in solventogenesis phase. This gene expression pattern was consistent with high butanol yield and low acid level in TS4-30. In summary, symbiotic system TS4-30 was obtained with butanol titer improvement and microoxygen tolerance.
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.|The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Chunkai
authors.initials: C
authors.lastname: Gu
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
authors.forename: Genyu
authors.initials: G
authors.lastname: Wang
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Mai
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Wu
authors.affiliation: The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122, China.
authors.forename: Jianrong
authors.initials: J
authors.lastname: Wu
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China.
authors.forename: Gehua
authors.initials: G
authors.lastname: Wang
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China. liuhongjuan@tsinghua.edu.cn.
authors.forename: Hongjuan
authors.initials: H
authors.lastname: Liu
authors.affiliation: Institute of Nuclear and New Energy Technology, Tsinghua University, Beijing, 100084, China. zhangja@tsinghua.edu.cn.
authors.forename: Jianan
authors.initials: J
authors.lastname: Zhang
mesh_terms: D000693:Anaerobiosis / Q000235:genetics; D001409:Bacillus cereus / Q000378:metabolism*; D056804:Biofuels / Q000382:microbiology*; D000440:Butanols / Q000378:metabolism*; D046969:Clostridium acetobutylicum / Q000378:metabolism*; D032621:DNA Shuffling; D005285:Fermentation; D015723:Gene Library; D009154:Mutation / Q000235:genetics
chemical_list: D056804:Biofuels; D000440:Butanols
keywords: Butanol fermentation; Genome shuffling; Real-time RT-PCR; Symbiotic system; TS4-30; TSH06