title: Quantifying CD95/cl-CD95L Implications in Cell Mechanics and Membrane Tension by Atomic Force Microscopy Based Force Measurements.
abstract: Atomic force microscopy (AFM) is an invaluable tool to investigate the structure of biological material surfaces by imaging them at nanometer scale in physiological conditions. It can also be used to measure the forces and mechanics from single molecule interaction to cell-cell adhesion. Here, we present a methodology that allows to quantify cell elastic properties (using the Young modulus) and cell membrane tension modulated by CD95/cl-CD95L interactions by coupling nanoindentation and membrane tube pulling using suitably decorated AFM levers.
authors.affiliation: Aix Marseille Université, LAI UM 61, Marseille, 13288, France.|Inserm, UMR_S 1067, Marseille, 13288, France.|CNRS, UMR 7333, Marseille, 13288, France.|Laboratoire Adhésion et Inflammation (LAI), Case 937 - 163, avenue de Luminyy, 13288, Marseille Cedex 09, France.
authors.forename: Anaïs
authors.initials: A
authors.lastname: Sadoun
authors.affiliation: Aix Marseille Université, LAI UM 61, Marseille, 13288, France. pierre-henri.puech@inserm.fr.|Inserm, UMR_S 1067, Marseille, 13288, France. pierre-henri.puech@inserm.fr.|CNRS, UMR 7333, Marseille, 13288, France. pierre-henri.puech@inserm.fr.|Laboratoire Adhésion et Inflammation (LAI), Case 937 - 163, avenue de Luminyy, 13288, Marseille Cedex 09, France. pierre-henri.puech@inserm.fr.
authors.forename: Pierre-Henri
authors.initials: PH
authors.lastname: Puech
mesh_terms: D001696:Biomechanical Phenomena; D002460:Cell Line; D002462:Cell Membrane / Q000378:metabolism*; D003627:Data Interpretation, Statistical; D055119:Elastic Modulus; D004548:Elasticity; D053222:Fas Ligand Protein / Q000378:metabolism*; D006801:Humans; D018625:Microscopy, Atomic Force / Q000379:methods; D013500:Surface Tension; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor
keywords: Atomic force microscopy; Cytoskeleton; Elasticity; Membrane tension; Membrane tubes; Young modulus