title: Flavoprotein miniSOG Cytotoxisity Can Be Induced By Bioluminescence Resonance Energy Transfer.
abstract: In this study, we investigated the possibility of phototoxic flavoprotein miniSOG (photosensitizer) excitation in cancer cells by bioluminescence occurring when luciferase NanoLuc oxidizes its substrate, furimazine. We have shown that the phototoxic flavoprotein miniSOG expressed in eukaryotic cells in fusion with NanoLuc luciferase is activated in the presence of its substrate, furimazine. Upon such condition, miniSOG possesses photoinduced cytotoxicity and causes a 48% cell death level in a stably transfected cell line.
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia.
authors.forename: E I
authors.initials: EI
authors.lastname: Shramova
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia.
authors.forename: G M
authors.initials: GM
authors.lastname: Proshkina
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Chumakov
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia.
authors.forename: Yu M
authors.initials: YM
authors.lastname: Khodarovich
authors.affiliation: Shemyakin/Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya Str., 16/10, 117997, Moscow, Russia ; National Research Tomsk Polytechnic University, Lenina av., 30, 634050, Tomsk, Russia.
authors.forename: S M
authors.initials: SM
authors.lastname: Deyev
keywords: NanoLuc luciferase; bioluminescence resonance energy transfer; flavoprotein miniSOG; photodynamic therapy