title: Analysis of Lignin Composition and Distribution Using Fluorescence Laser Confocal Microspectroscopy.
abstract: Lignin is a polyphenolic polymer specifically accumulating in the cell walls of xylem cells in higher vascular plants. Far from being homogeneous, the lignification of xylem cell walls varies in deposition site, quantity, composition and macromolecular conformation depending on the cell wall compartment, cell type, cell developmental stage and plant species. Here, we describe how confocal microspectroscopy methods using lignin autofluorescence can be used to evaluate the relative lignin amounts, its spatial distribution and composition at the cellular and sub-cellular levels in both isolated cells and histological cross-sections of plant tissues.
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Decou
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.|Department of Medical Biochemistry and Biophysics, Umeå University, 901 87, Umeå, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Serk
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.|Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91, Stockholm, Sweden.
authors.forename: Delphine
authors.initials: D
authors.lastname: Ménard
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden. edouard.pesquet@su.se.|Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91, Stockholm, Sweden. edouard.pesquet@su.se.
authors.forename: Edouard
authors.initials: E
authors.lastname: Pesquet
mesh_terms: D019295:Computational Biology / Q000379:methods; D007091:Image Processing, Computer-Assisted; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism*; D018613:Microscopy, Confocal / Q000379:methods; D012984:Software; D014018:Tissue Distribution; D014934:Wood / Q000737:chemistry / Q000378:metabolism; D052584:Xylem / Q000737:chemistry / Q000378:metabolism
chemical_list: D008031:Lignin
keywords: Fibers; Fluorescence confocal microspectroscopy; Lignin; Tracheary elements; Vessels; Wood; Xylem