title: Identification and Characterization of a High Efficiency Aniline Resistance and Degrading Bacterium MC-01.
abstract: Biodegradation is one of the important methods for the treatment of industrial wastewater containing aniline. In this paper, a degrading bacterium named MC-01, which could survive in high concentration aniline wastewater, was screened from industrial wastewater containing aniline and sludge. MC-01 was preliminarily identified as Ochrobactrum sp. based on the amplified 16S rDNA gene sequence and Biolog system identification. MC-01 was highly resistant to aniline. After 24-h culture under aniline concentration of 6500 mg/L, the amount of bacterium survived still remained 0.05 × 106CFU/mL. Experiments showed that there was no coupling expression between the growth of MC-01 and aniline degradation. The optimum growth conditions in LB culture were pH 6.0, 30 °C of temperature, and 4% of incubation amount, respectively. And the optimum conditions of aniline degradation of MC-01 were pH 7.0, 45 °C of temperature, and 3.0% of salt concentration, respectively. The degradation rate of MC-01 (48 h) in different aniline concentrations (200~1600 mg/L) was stable under the optimum conditions, which could reach more than 75%.
authors.affiliation: Nanjing University of Technology, College of Food Science and Light Industry, Nanjing, 211816, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: Nanjing University of Technology, College of Food Science and Light Industry, Nanjing, 211816, China.
authors.forename: Chen
authors.initials: C
authors.lastname: Ying
authors.affiliation: Nanjing University of Technology, College of Food Science and Light Industry, Nanjing, 211816, China. nifang@njtech.edu.cn.
authors.forename: Ni
authors.initials: N
authors.lastname: Fang
authors.affiliation: Nanjing University of Technology, College of Food Science and Light Industry, Nanjing, 211816, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Zhong
authors.affiliation: Nanjing University of Technology, College of Food Science and Light Industry, Nanjing, 211816, China.
authors.forename: Zhong
authors.initials: Z
authors.lastname: Zhao-Xiang
authors.affiliation: Nanjing University of Technology, College of Food Science and Light Industry, Nanjing, 211816, China.
authors.forename: Sun
authors.initials: S
authors.lastname: Yun
mesh_terms: D000814:Aniline Compounds / Q000378:metabolism*; D001673:Biodegradation, Environmental; D006801:Humans; D006863:Hydrogen-Ion Concentration; D007220:Industrial Waste; D042041:Ochrobactrum / Q000145:classification / Q000235:genetics / Q000302:isolation & purification* / Q000378:metabolism; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics*; D013696:Temperature; D062065:Wastewater / Q000382:microbiology*; D014874:Water Pollutants, Chemical / Q000378:metabolism*
chemical_list: D000814:Aniline Compounds; D007220:Industrial Waste; D012336:RNA, Ribosomal, 16S; D062065:Wastewater; D014874:Water Pollutants, Chemical; C023650:aniline
keywords: Aniline degradation; Coupling expression; Identification; Microorganism; Screen