title: Cryogenic optical localization provides 3D protein structure data with Angstrom resolution.
abstract: We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.
authors.affiliation: Max Planck Institute for the Science of Light, Erlangen, Germany.|Department of Physics, Friedrich Alexander University (FAU) Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Siegfried
authors.identifier: 0000-0003-2044-0372
authors.initials: S
authors.lastname: Weisenburger
authors.affiliation: Max Planck Institute for the Science of Light, Erlangen, Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Boening
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Schomburg
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Giller
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Stefan
authors.initials: S
authors.lastname: Becker
authors.affiliation: Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Griesinger
authors.affiliation: Max Planck Institute for the Science of Light, Erlangen, Germany.|Department of Physics, Friedrich Alexander University (FAU) Erlangen-Nürnberg, Erlangen, Germany.
authors.forename: Vahid
authors.initials: V
authors.lastname: Sandoghdar
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry / Q000378:metabolism; D001665:Binding Sites; D001710:Biotin / Q000737:chemistry / Q000378:metabolism; D018360:Crystallography, X-Ray; D005456:Fluorescent Dyes / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005615:Freezing; D056506:Geobacillus / Q000737:chemistry; D000071677:Histidine Kinase / Q000737:chemistry* / Q000378:metabolism; D007091:Image Processing, Computer-Assisted / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D008856:Microscopy, Fluorescence / Q000379:methods*; D055095:Optics and Photonics / Q000295:instrumentation / Q000379:methods*; D011487:Protein Conformation; D000072417:Protein Domains; D000072760:Single Molecule Imaging / Q000379:methods*; D013269:Stochastic Processes; D019809:Streptavidin / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D005456:Fluorescent Dyes; D001710:Biotin; D019809:Streptavidin; D000071677:Histidine Kinase