title: [Activation of biosynthesis of guanyl-specific ribonuclease secreted by Bacillus circulans under salt stress].
abstract: The gene transcription of guanyl-specific ribonucleases (RNases), which provide available phosphate to cells of Bacillus, is controlled by the signal transduction system PhoP-PhoR. However, the biosynthesis of B. circulans RNase does not depend on the signal-transduction regulatory proteins of Pho regulon. It has been found that raising the salt molar concentration in culture medium increases the level of extracellular guanyl-specific ribonuclease Bci synthesized by B. circulans. Sequences homologous to the binding sites of the regulatory protein DegU were found in RNase Bci promoter. The functioning of the DegS-DegU signal transduction system is stimulated by a high salt concentration. Using a strain of B. subtilis that is defective in the DegU regulatory protein, we have shown that the DegS-DegU system participates in the regulation of RNase Bci expression under salt stress.
authors.affiliation: Kazan Federal University, Kazan, 420008 Russia.|Maya_Kharitonova@mail.ru.
authors.forename: M A
authors.initials: MA
authors.lastname: Kharitonova
authors.affiliation: Kazan State Medical Academy, Ministry of Health of the Russian Federation, Kazan, 420012 Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Kipenskaya
authors.affiliation: Kazan Federal University, Kazan, 420008 Russia.
authors.forename: O N
authors.initials: ON
authors.lastname: Ilinskaya
mesh_terms: D001407:Bacillus / Q000201:enzymology*; D001426:Bacterial Proteins / Q000378:metabolism; D004789:Enzyme Activation / Q000502:physiology; D015964:Gene Expression Regulation, Bacterial / Q000502:physiology*; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D018085:Regulon / Q000502:physiology; D012260:Ribonucleases / Q000378:metabolism*; D013312:Stress, Physiological / Q000502:physiology*
chemical_list: D001426:Bacterial Proteins; C066496:DegS protein, Bacteria; C066497:DegU protein, Bacteria; C061279:PhoR protein, Bacteria; C061064:PhoP protein, Bacteria; D012260:Ribonucleases
keywords: Bacillus circulans; biosynthesis activation; guanyl-specific ribonuclease; salt stress