title: Screening, purification, and characterization of a novel organic solvent-tolerant esterase, Lip2, from Monascus purpureus strain M7.
abstract: Organic solvent-tolerant esterases are proven to be excellent biocatalysts in chemical and pharmaceutical industries. A novel organic solvent-tolerant esterase gene, lip2, was isolated from filamentous fungi Monascus purpureus M7. The sequence analysis suggested that lip2 has a conserved "GDSL" motif near the active center. The multiple-sequence alignment and phylogenetic analysis revealed that Lip2 displayed two unique amino-acid sequence motifs that clearly separate it from any other previously described lipase family. After incubation in 20% methanol and ethanol for 3 h, the Lip2 displayed 190 and 180% residual activities, respectively. It retained 99-110% relative activity in 20% (v/v) hydrophilic organic solvents after incubation for 1 day. This esterase showed optimal activity at 40 °C and retained about 70% maximal activity at 60 °C. The enzyme also displayed more than 50% residual activity over a range of pH 5-11. In the presence of most of metal ions or additives, Lip2 retained most of the activity. These unique properties of Lip2 make it a promising as biocatalyst for industrial processes.
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|National Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Li-Jing
authors.initials: LJ
authors.lastname: Kang
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|National Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Zi-Tong
authors.initials: ZT
authors.lastname: Meng
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|National Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Chen
authors.initials: C
authors.lastname: Hu
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|National Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|National Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China.|College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
authors.forename: Hai-Lun
authors.initials: HL
authors.lastname: Guo
authors.affiliation: System Design Institute of Hubei Aerospace Technology Academy, Wuhan, 430040, People's Republic of China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. limu@mail.hzau.edu.cn.|National Key Laboratory of Agro-Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, People's Republic of China. limu@mail.hzau.edu.cn.|College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China. limu@mail.hzau.edu.cn.
authors.forename: Mu
authors.initials: M
authors.lastname: Li
mesh_terms: D020816:Amino Acid Motifs; D004795:Enzyme Stability; D005656:Fungal Proteins / Q000737:chemistry* / Q000302:isolation & purification*; D006358:Hot Temperature; D006863:Hydrogen-Ion Concentration; D008049:Lipase / Q000737:chemistry* / Q000302:isolation & purification*; D032741:Monascus / Q000201:enzymology*; D012997:Solvents / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D012997:Solvents; D008049:Lipase
keywords: Enzyme application; Enzyme stability; Enzymes; Gene cloning and expression; Isolation and characterization