title: Nitratireductor aestuarii sp. nov., a marine alphaproteobacterium isolated from an estuary.
abstract: Two bacterial strains, 2-2-12-1T and 2-2-12-2, were isolated from the estuary of the Jiulong River, south-east China. Cells were Gram-stain-negative, non-motile, short rods without flagella. Growth occurred at 25-45 °C, at pH 5.0-9.0 and with 0.5-2 % (w/v) NaCl. The bacteria were unable to reduce nitrate. The predominant fatty acids were C19 : 0 cyclo ω9c and C18 : 1ω7c. Phylogenetic analysis based on 16S rRNA gene sequences showed that both strains belong to the genus Nitratireductor, family Phyllobacteriaceae, class Alphaproteobacteria. Their closest neighbours were 'Nitratireductor lucknowense' DSM 24322 (96.3 and 96.5 % similarity, respectively) and Nitratireductor pacificus MCCC 1A01024T (96.2 and 96.3 % similarity, respectively). The DNA G+C contents of the two strains were 56.7 mol%. DNA-DNA hybridization between strain 2-2-12-1T and the two most closely related type strains revealed 57.3 and 52.3 % relatedness, respectively. Evidence from genotypic, chemotaxonomic and phenotypic data indicated that strains 2-2-12-1T and 2-2-12-2 represent a novel species of the genus Nitratireductor, for which the name Nitratireductor aestuarii sp. nov. is proposed. The type strain is 2-2-12-1T (=LMG 29090T=CGMCC 1.15320T).
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Danyun
authors.initials: D
authors.lastname: Ou
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Hao
authors.initials: H
authors.lastname: Huang
authors.affiliation: Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
authors.forename: Renao
authors.initials: R
authors.lastname: Bai
authors.affiliation: Engineering Research Center of Molecular Diagnostics, Ministry of Education, School of Life Sciences, Xiamen University, Xiamen 361102, PR China.
authors.forename: Qingge
authors.initials: Q
authors.lastname: Li
authors.affiliation: Key Laboratory of Microbial Engineering at the Institute of Biology, Henan Academy of Sciences, Zhengzhou 450008, PR China.
authors.forename: Yanan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Environmental Microbiology and Ecology Research Center, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, PR China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Yin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D063366:Estuaries; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D059127:Phyllobacteriaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S