title: Biological roles of glial fibrillary acidic protein as a biomarker in cartilage regenerative medicine.
abstract: Glial fibrillary acidic protein (GFAP) is an intermediate filament that is expressed in specifically expressed auricular chondrocytes, which are good cell sources of cartilage regenerative medicine. Although our group uses GFAP as a biomarker of matrix production in the cultured auricular chondrocytes, the biological roles of GFAP in auricular chondrocytes has remained unknown. In this study, we demonstrated the biological functions of GFAP in the human and mouse derived auricles to clarify the significance and role with the chondrocytes of GFAP in order to provide useful information for reliable and safe regenerative medicine. We examined the cell responses to stretch stress for these chondrocytes and completed a nuclear morphological analysis. Based on these results, GFAP seems to support the resistance to severe mechanical stress in the tissue which physiologically suffers from a stretch overload, and plays pivotal roles in the conservation of cell structures and functions through the maintenance of nuclear morphology.
authors.affiliation: Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.|Faculty of Medicine, Department of Oral and Maxillofacial Surgery, The University of Tokyo, Tokyo, Japan.
authors.forename: Sanshiro
authors.identifier: http://orcid.org/0000-0001-5520-7488
authors.initials: S
authors.lastname: Kanazawa
authors.affiliation: Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
authors.forename: Satoru
authors.initials: S
authors.lastname: Nishizawa
authors.affiliation: Faculty of Medicine, Department of Oral and Maxillofacial Surgery, The University of Tokyo, Tokyo, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Takato
authors.affiliation: Department of Cartilage and Bone Regeneration (Fujisoft), Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.|Faculty of Medicine, Department of Oral and Maxillofacial Surgery, The University of Tokyo, Tokyo, Japan.
authors.forename: Kazuto
authors.initials: K
authors.lastname: Hoshi
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D053144:Cell Nucleus Shape; D048429:Cell Size; D002478:Cells, Cultured; D002648:Child; D019902:Chondrocytes / Q000378:metabolism*; D020219:Chondrogenesis; D004425:Ear Cartilage / Q000166:cytology / Q000378:metabolism*; D005838:Genotype; D005904:Glial Fibrillary Acidic Protein / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D040542:Mechanotransduction, Cellular; D008810:Mice, Inbred C57BL; D010641:Phenotype; D044968:Regenerative Medicine / Q000379:methods*; D013314:Stress, Mechanical; D014162:Transfection
chemical_list: D015415:Biomarkers; D005904:Glial Fibrillary Acidic Protein
keywords: GFAP; auricular chondrocyte; cytoskeleton; regenerative medicine