title: Preparation of DNA Substrates and Functionalized Glass Surfaces for Correlative Nanomanipulation and Colocalization (NanoCOSM) of Single Molecules.
abstract: Simultaneous nanomanipulation and colocalization of single molecules (NanoCOSM) provides a unique opportunity to correlate the mechanical properties and activities of biomolecules with their conformational states or states of assembly as part of dynamic macromolecular complexes. This opens the door to real-time single-molecule analysis of the correlations between structure, function, and composition of large multicomponent protein complexes.
authors.affiliation: Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France.
authors.forename: C
authors.initials: C
authors.lastname: Duboc
authors.affiliation: Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France.
authors.forename: J
authors.initials: J
authors.lastname: Fan
authors.affiliation: Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France.
authors.forename: E T
authors.initials: ET
authors.lastname: Graves
authors.affiliation: Institut Jacques Monod, Centre National de la Recherche Scientifique, University of Paris Diderot and Sorbonne Paris Cité, Paris, France; Ecole Normale Supérieure, Institut de Biologie de l'ENS (iBENS), INSERM, CNRS, PSL Research University, Paris, France. Electronic address: strick@ijm.univ-paris-diderot.fr.
authors.forename: T R
authors.initials: TR
authors.lastname: Strick
mesh_terms: D004247:DNA / Q000737:chemistry; D005898:Glass / Q000737:chemistry; D046911:Macromolecular Substances / Q000737:chemistry* / Q000302:isolation & purification; D018625:Microscopy, Atomic Force / Q000379:methods*; D036103:Nanotechnology / Q000379:methods*; D000072760:Single Molecule Imaging / Q000379:methods*
chemical_list: D046911:Macromolecular Substances; D004247:DNA
keywords: Correlative methods; DNA; NanoCOSM; Single molecules; Single-molecule fluorescence; Single-molecule nanomanipulation