title: Click and Fluoresce: A Bioorthogonally Activated Smart Probe for Wash-Free Fluorescent Labeling of Biomolecules.
abstract: Bioorthogonally activated smart probes greatly facilitate the selective labeling of biomolecules in living system. Herein, we described a novel type of smart probes with tunable reaction rates, high fluorescence turn-on ratio, and easy access. The practicality of such probes was demonstrated by selective labeling of lipid and hCAII in Hela cells.
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30303 United States.
authors.forename: Xingyue
authors.initials: X
authors.lastname: Ji
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30303 United States.
authors.forename: Kaili
authors.initials: K
authors.lastname: Ji
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30303 United States.
authors.forename: Vayou
authors.initials: V
authors.lastname: Chittavong
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30303 United States.
authors.forename: Robert E
authors.initials: RE
authors.lastname: Aghoghovbia
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30303 United States.
authors.forename: Mengyuan
authors.initials: M
authors.lastname: Zhu
authors.affiliation: Department of Chemistry and Center for Diagnostics and Therapeutics, Georgia State University , Atlanta, Georgia 30303 United States.
authors.forename: Binghe
authors.identifier: 0000-0002-2200-5270
authors.initials: B
authors.lastname: Wang
mesh_terms: D024402:Carbonic Anhydrase II / Q000737:chemistry* / Q000378:metabolism; D057930:Click Chemistry; D005453:Fluorescence; D005456:Fluorescent Dyes / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry*; D015394:Molecular Structure; D061848:Optical Imaging
chemical_list: D005456:Fluorescent Dyes; D008055:Lipids; D024402:Carbonic Anhydrase II