title: Detection of Zika Virus in Desiccated Mosquitoes by Real-Time Reverse Transcription PCR and Plaque Assay.
abstract: We assayed Zika virus-infected mosquitoes stored at room temperature for <30 days for live virus by using plaque assay and virus RNA by using real-time reverse transcription PCR. Viable virus was detected in samples stored <10 days, and virus RNA was detected in samples held for 30 days.
authors.forename: Kristen L
authors.initials: KL
authors.lastname: Burkhalter
authors.forename: Harry M
authors.initials: HM
authors.lastname: Savage
mesh_terms: D000330:Aedes / Q000821:virology*; D000818:Animals; D002522:Chlorocebus aethiops; D003890:Desiccation; D005260:Female; D006801:Humans; D012367:RNA, Viral / Q000302:isolation & purification; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*; D020133:Reverse Transcriptase Polymerase Chain Reaction / Q000379:methods*; D014709:Vero Cells; D010948:Viral Plaque Assay / Q000379:methods*; D000071244:Zika Virus / Q000302:isolation & purification*
chemical_list: D012367:RNA, Viral
keywords: Culicidae; Vero cells; Zika virus; arboviruses; cold chain; mosquitoes; plaque assay; real-time reverse transcription PCR; virus RNA; virus detection; virus persistence in mosquitoes; viruses