title: Evaluation of Antibacterial, Antineoplastic, and Immunomodulatory Activity ofPaullinia cupanaSeeds Crude Extract and Ethyl-Acetate Fraction.
abstract: Paullinia cupana(Guarana) is a native plant of Amazon region that has very traditional importance. Its seeds are rich in bioactive compounds, including tannins, which exhibit relevant properties.Objective.This study aimed to evaluate antibacterial, antineoplastic, and immunomodulatory activity ofP. cupanaseeds crude extract (CE) and ethyl-acetate fraction (EAF).Methods.Antibacterial activity was evaluated by determination of minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC). Antineoplastic activity was evaluated by MTT assays in hepatocellular carcinoma (HepG2), breast adenocarcinoma (MCF-7), ductal carcinoma (T47-D), non-Hodgkin's B cell lymphoma (Toledo), T cell leukemia (Jukart), and Acute Leukemia (HL-60) cell lines. BALB/c mice splenocytes were treated to assess IFN-γ, IL-6, IL-17, and IL-10 levels by sandwich ELISA.Results.CE and EAF were not toxic to peripheral blood cells and splenocytes. CE and EAF fractions showed a bacteriostatic activity (MIC = 250μg/mL) and presented IC50values of 70.25μg/mL and 61.18μg/mL in HL-60 leukemia cell line. All cytokines evaluated had their levels reduced after treatment, following dose-response model.Discussion and Conclusion.Different biological activities were observed for both CE and EAF, suggestingP. cupanaas a source of bioactive substances, especially tannins that may be used for several diseases treatments.
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Lidiane Vasconcelos do Nascimento
authors.initials: LV
authors.lastname: Carvalho
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Marina Ferraz
authors.initials: MF
authors.lastname: Cordeiro
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Thiago Ubiratan Lins
authors.initials: TU
authors.lastname: E Lins
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Maria Clara Pinheiro Duarte
authors.initials: MC
authors.lastname: Sampaio
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Gabriela Souto Vieira
authors.initials: GS
authors.lastname: de Mello
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Valécia de Cassia Mendonça
authors.initials: VC
authors.lastname: da Costa
authors.affiliation: Pharmacy Department, Maringa State University, Maringa, PR, Brazil.
authors.forename: Leila Larisa Medeiros
authors.initials: LL
authors.lastname: Marques
authors.affiliation: Pharmacy Department, Ponta Grossa State University, Ponta Grossa, PR, Brazil.
authors.forename: Traudi
authors.initials: T
authors.lastname: Klein
authors.affiliation: Pharmacy Department, Maringa State University, Maringa, PR, Brazil.
authors.forename: João Carlos Palazzo
authors.initials: JC
authors.lastname: de Mello
authors.affiliation: Microbiology and Immunology Laboratory of Academic Center of Vitória, Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Isabella Macário Ferro
authors.initials: IM
authors.lastname: Cavalcanti
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Ivan da Rocha
authors.initials: ID
authors.lastname: Pitta
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Maira
authors.identifier: 0000-0001-5881-0338
authors.initials: M
authors.lastname: Galdino da Rocha Pitta
authors.affiliation: Laboratory of Immunomodulation and New Therapeutical Approaches, Research Centre for Therapeutic Innovation Suely Galdino (NUPIT-SG), Federal University of Pernambuco, Recife, PE, Brazil.
authors.forename: Moacyr Jesus Barreto de Melo
authors.identifier: 0000-0002-1883-6012
authors.initials: MJ
authors.lastname: Rêgo