title: Controlled Retention of BMP-2-Derived Peptide on Nanofibers Based on Mussel-Inspired Adhesion for Bone Formation.
abstract: Although bone morphogenetic protein-2 (BMP-2) has been frequently used to stimulate bone formation, it has several side effects to be addressed, including the difficulty in optimization of clinically relevant doses and unwanted induction of cancerous signaling processes. In this study, an osteogenic peptide (OP) derived from BMP-2 was investigated as a substitute for BMP-2. In vitro studies showed that OP was able to enhance the osteogenic differentiation and mineralization of human mesenchymal stem cells (hMSCs). The peptides were then conjugated onto biocompatible poly-ι-lactide electrospun nanofibers through polydopamine chemistry. Surface chemical analysis proved that more than 80% of the peptides were stably retained on the nanofiber surface after 8 h of polydopamine coating during at least 28 days, and the amount of peptides that was retained increased depending on the polydopamine coating time. For instance, about 65% of the peptides were retained on nanofibers after 4 h of polydopamine coating. Also, a relatively small dose of peptides could effectively induce bone formation in in vivo critical-sized defects on the calvarial bones of mice. More than 50.4% ± 16.9% of newly formed bone was filled within the defect after treatment with only 10.5 ± 0.6 μg of peptides. Moreover, these groups had similar elastic moduli and contact hardnesses with host bone. Taken together, our results suggest that polydopamine-mediated OP immobilized on nanofibers can modulate the retention of relatively short lengths of peptides, which might make this an effective therapeutic remedy to guide bone regeneration using a relatively small amount of peptides.
authors.affiliation: 1 Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University , Seoul, Republic of Korea.|2 BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University , Seoul, Republic of Korea.
authors.forename: Jinkyu
authors.initials: J
authors.lastname: Lee
authors.affiliation: 1 Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University , Seoul, Republic of Korea.|2 BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University , Seoul, Republic of Korea.
authors.forename: Sajeesh Kumar Madhurakkat
authors.initials: SK
authors.lastname: Perikamana
authors.affiliation: 1 Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University , Seoul, Republic of Korea.|2 BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University , Seoul, Republic of Korea.
authors.forename: Taufiq
authors.initials: T
authors.lastname: Ahmad
authors.affiliation: 3 Department of Nanobio Medical Science, Dankook University , Chonan, Republic of Korea.
authors.forename: Min Suk
authors.initials: MS
authors.lastname: Lee
authors.affiliation: 3 Department of Nanobio Medical Science, Dankook University , Chonan, Republic of Korea.
authors.forename: Hee Seok
authors.initials: HS
authors.lastname: Yang
authors.affiliation: 4 Division of Orthodontics, College of Dentistry, The Ohio State University , Columbus, Ohio.
authors.forename: Do-Gyoon
authors.initials: DG
authors.lastname: Kim
authors.affiliation: 5 Division of Bioengineering, College of Life Sciences and Bioengineering, Incheon National University , Incheon, Republic of Korea.
authors.forename: Kyobum
authors.initials: K
authors.lastname: Kim
authors.affiliation: 6 Wooridul Life Sciences & WINNOVA Research Institute , Seoul, Republic of Korea.
authors.forename: Bosun
authors.initials: B
authors.lastname: Kwon
authors.affiliation: 1 Department of Bioengineering, Institute for Bioengineering and Biopharmaceutical Research, Hanyang University , Seoul, Republic of Korea.|2 BK21 Plus Future Biopharmaceutical Human Resources Training and Research Team, Hanyang University , Seoul, Republic of Korea.
authors.forename: Heungsoo
authors.initials: H
authors.lastname: Shin
mesh_terms: D000818:Animals; D055396:Bone Morphogenetic Protein 2; D001861:Bone Regeneration / Q000187:drug effects; D002478:Cells, Cultured; D005260:Female; D006801:Humans; D007211:Indoles / Q000737:chemistry*; D059630:Mesenchymal Stem Cells / Q000166:cytology*; D051379:Mice; D057139:Nanofibers / Q000737:chemistry*; D010455:Peptides / Q000737:chemistry* / Q000494:pharmacology*; D011108:Polymers / Q000737:chemistry*; D054457:Tissue Scaffolds
chemical_list: D055396:Bone Morphogenetic Protein 2; D007211:Indoles; D010455:Peptides; D011108:Polymers; C568283:polydopamine
keywords: electrospinning; nanofiber; osteogenic differentiation; osteogenic peptide; polydopamine