title: Weissella cibaria WIKIM28 ameliorates atopic dermatitis-like skin lesions by inducing tolerogenic dendritic cells and regulatory T cells in BALB/c mice.
abstract: The occurrence of atopic dermatitis (AD), a chronic inflammatory skin disease, has been increasing steadily in children and adults in recent decades. In this study, we evaluated the ability of the lactic acid bacterium Weissella cibaria WIKIM28 isolated from gatkimchi, a Korean fermented vegetable preparation made from mustard leaves, to suppress the development of AD induced by 2,4-dinitrochlorobenzene in a murine model. Oral administration of W. cibaria WIKIM28 reduced AD-like skin lesions, epidermal thickening, and serum immunoglobulin E levels. Furthermore, the production of type 2 helper T (Th2) cytokines such as interleukin (IL)-4, IL-5, and IL-13 decreased in peripheral lymph node cells. Moreover, the intake of W. cibaria WIKIM28 increased the proportion of CD4+CD25+Foxp3+regulatory T (Treg) cells in mesenteric lymph nodes (MLNs) and IL-10 levels in polyclonally stimulated MLN cells. In conclusion, the oral administration of W. cibaria WIKIM28 isolated from gatkimchi ameliorated AD-like symptoms by suppressing allergic Th2 responses and inducing Treg responses. These results suggest that W. cibaria WIKIM28 may be applicable as a probiotic for the prevention and amelioration of AD.
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Seul Ki
authors.initials: SK
authors.lastname: Lim
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Min-Sung
authors.initials: MS
authors.lastname: Kwon
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Lee
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Young Joon
authors.initials: YJ
authors.lastname: Oh
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Ja-Young
authors.initials: JY
authors.lastname: Jang
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Jong-Hee
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Advanced Process Technology and Fermentation Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Hae Woong
authors.initials: HW
authors.lastname: Park
authors.affiliation: Research Group of Gut Microbiome, Korea Food Research Institute, Seongnam 13539, Republic of Korea.
authors.forename: Young-Do
authors.initials: YD
authors.lastname: Nam
authors.affiliation: Division of Bioengineering, Incheon National University, Incheon 22012, Republic of Korea.
authors.forename: Myung-Ji
authors.initials: MJ
authors.lastname: Seo
authors.affiliation: Biological Disaster Analysis Group, Korea Basic Science Institute, Daejeon 34133, Republic of Korea.
authors.forename: Seong Woon
authors.initials: SW
authors.lastname: Roh
authors.affiliation: Microbiology and Functionality Research Group, World Institute of Kimchi, Gwangju 61755, Republic of Korea.
authors.forename: Hak-Jong
authors.initials: HJ
authors.lastname: Choi
mesh_terms: D000284:Administration, Oral; D000818:Animals; D001426:Bacterial Proteins / Q000008:administration & dosage* / Q000302:isolation & purification; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D003876:Dermatitis, Atopic / Q000139:chemically induced / Q000188:drug therapy* / Q000473:pathology; D004195:Disease Models, Animal; D007108:Immune Tolerance; D007073:Immunoglobulin E / Q000097:blood; D007155:Immunologic Factors / Q000008:administration & dosage* / Q000302:isolation & purification; D008807:Mice, Inbred BALB C; D012867:Skin / Q000473:pathology; D050378:T-Lymphocytes, Regulatory / Q000187:drug effects / Q000276:immunology*; D016896:Treatment Outcome; D058836:Weissella / Q000737:chemistry*
chemical_list: D001426:Bacterial Proteins; D007155:Immunologic Factors; D007073:Immunoglobulin E