title: Changes of Ovarian microRNA Profile in Long-Living Ames Dwarf Mice during Aging.
abstract: The Ames dwarf (df/df) mice have extended longevity and can preserve the ovarian reserve longer than Normal (N) mice. Based on this, the aim of our study was to evaluate the ovarian microRNA (miRNA) profile in young and aged df/df and N mice. Ovarian tissue was collected at 5-6 months and at 21-22 months of age for miRNA sequencing. We detected a total of 404 miRNAs in the ovarian samples, from which the abundance of 22 and 33 miRNAs changed with age in N and df/df mice, respectively. Of these, only three miRNAs were commonly regulated with age between N and df/df mice, indicating a very divergent miRNA profile between genotypes. We also detected that 46 miRNAs were regulated between N and df/df mice, of which 23 were regulated exclusively in young mice, 12 exclusively in old mice and 12 commonly regulated at young and old ages. Many genes likely to be targeted by these miRNAs are involved in the FoxO, mTOR, PI3k/Akt and insulin signaling pathways. These results suggest that the aging process has a differential impact on the ovarian miRNA profile in df/df mice, and suggest that these miRNAs can be central players in the maintenance of a younger ovarian phenotype.
authors.affiliation: Faculdade de Nutrição, Universidade Federal de Pelotas, Pelotas, RS, Brazil.|College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, United States of America.
authors.forename: Augusto
authors.initials: A
authors.lastname: Schneider
authors.affiliation: Center for Pharmacogenomics, Department of Medicine, Washington University School of Medicine, St. Louis, MO, United States of America.
authors.forename: Scot J
authors.initials: SJ
authors.lastname: Matkovich
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, United States of America.
authors.forename: Berta
authors.initials: B
authors.lastname: Victoria
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, United States of America.
authors.forename: Lina
authors.initials: L
authors.lastname: Spinel
authors.affiliation: Departments of Internal Medicine and Physiology, Southern Illinois University School of Medicine, Springfield, IL, United States of America.
authors.forename: Andrzej
authors.initials: A
authors.lastname: Bartke
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, United States of America.|Department of Biology and Environmental Studies, Poznan University of Medical Sciences, Poznan, Poland.|Department of Head and Neck Surgery, The Greater Poland Cancer Centre, Poznan, Poland.
authors.forename: Pawel
authors.initials: P
authors.lastname: Golusinski
authors.affiliation: College of Medicine, Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL, United States of America.|Department of Head and Neck Surgery, The Greater Poland Cancer Centre, Poznan, Poland.
authors.forename: Michal M
authors.initials: MM
authors.lastname: Masternak
mesh_terms: D000375:Aging / Q000235:genetics*; D000818:Animals; D016000:Cluster Analysis; D005260:Female; D020869:Gene Expression Profiling; D018507:Gene Expression Regulation, Developmental; D063990:Gene Ontology; D008136:Longevity / Q000235:genetics*; D051379:Mice; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010053:Ovary / Q000378:metabolism*
chemical_list: D035683:MicroRNAs