title: Aminoacyl-tRNA-Utilizing Enzymes in Natural Product Biosynthesis.
abstract: Aminoacyl-tRNAs were long thought to be involved solely in ribosome-dependent protein synthesis and essential primary metabolism processes, such as targeted protein degradation and peptidoglycan synthesis. About 10 years ago, an aminoacyl-tRNA-dependent enzyme involved in the biosynthesis of the antibiotic valanimycin was discovered in a Streptomyces strain. Far from being an isolated case, this discovery has been followed by the description of an increasing number of aminoacyl-tRNA-dependent enzymes involved in secondary metabolism. This review describes the three groups of aminoacyl-tRNA-dependent enzymes involved in the synthesis of natural products. Each group is characterized by a particular chemical reaction, and its members are predicted to share a specific fold. The three groups are cyclodipeptide synthases involved in diketopiperazine synthesis, LanB-like dehydratases involved in the posttranslational modification of ribosomal peptides, and transferases from various biosynthesis pathways.
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91198, Gif-sur-Yvette Cedex, France.
authors.forename: Mireille
authors.initials: M
authors.lastname: Moutiez
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91198, Gif-sur-Yvette Cedex, France.
authors.forename: Pascal
authors.initials: P
authors.lastname: Belin
authors.affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay , 91198, Gif-sur-Yvette Cedex, France.
authors.forename: Muriel
authors.identifier: http://orcid.org/0000-0003-0398-3404
authors.initials: M
authors.lastname: Gondry
mesh_terms: D001688:Biological Products / Q000378:metabolism*; D002384:Catalysis; D004798:Enzymes / Q000737:chemistry / Q000378:metabolism*; D011487:Protein Conformation; D012346:RNA, Transfer, Amino Acyl / Q000378:metabolism*; D013379:Substrate Specificity
chemical_list: D001688:Biological Products; D004798:Enzymes; D012346:RNA, Transfer, Amino Acyl