title: Analysis of anti-bacterial and anti oxidative activity ofAzadirachta indicabark using various solvents extracts.
abstract: Herbal medications have been used for relief of symptoms of disease. Regardless of the great advances observed in current medicine in recent decades, plants still make a significant contribution to health care. An alarming increase in bacterial strains resistant to a number of antimicrobial agents demands that a renewed effort be made to seek antibacterial agents effective against pathogenic bacteria resistant to or less sensitive to current antibiotics. Anti-bacterial activity ofAzadirachta indicastem bark was tested against pathogenicSalmonella paratyphiandSalmonella typhiusing various solvent extracts. The in vitro anti-bacterial activity was performed by agar well diffusion method and the results were expressed as the average diameter of zone of inhibition of bacterial growth around the well. The ethanol and methanol extracts showed better anti-bacterial activity with zone of inhibition (20-25 mm) when compared with other tested extracts and standard antibiotic Erythromycin (15 mcg) with zone of inhibition (13-14 mm). Using Fisher's exact test of significance difference was found between two Salmonella strains sensitivity patterns against tested extracts (P⩽ 0.035). Extracts ofA. indicastem bark also exhibited significant antioxidant activity, thus establishing the extracts as an antioxidant. The results obtained in this study give some scientific support to theA. indicastem bark for further investigation of compounds and in future could be used as drug.
authors.affiliation: Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Raid
authors.initials: R
authors.lastname: Al Akeel
authors.affiliation: Central Research Institute of Unani Medicine, Hyderabad 500 038, India.
authors.forename: Ayesha
authors.initials: A
authors.lastname: Mateen
authors.affiliation: P.G. Department of Biotechnology, A.V. College, Hyderabad, A.P., India.
authors.forename: K
authors.initials: K
authors.lastname: Janardhan
authors.affiliation: Central Research Institute of Unani Medicine, Hyderabad 500 038, India.
authors.forename: V C
authors.initials: VC
authors.lastname: Gupta
keywords: Anti-bacterial activity; Anti-oxidant activity; Azadirachta indica