title: Staphylococcal enterotoxin B administration in pregnant rats alters the splenic lymphocyte response in adult offspring rats.
abstract: Our previous study suggested that SEB exposure in pregnant rats could lead to the change of T cells subpopulation in both peripheral blood and thymus of the offspring rats. However, rarely is known about the influence of SEB exposure in pregnant rats on T cell subpopulation in the spleens of offspring rats.
  
  
  
  SEB was intravenously administered to the pregnant rats at gestational day 16 in this study. The percentages, in vivo and in vitro responses of CD4 and CD8 T cells were investigated with flow cytometry. The prenatal SEB exposure obviously increased splenic CD4 T cell percentages of both neonates and adult offspring rats, and obviously reduced splenic CD8 T cell percentages of both the fifth day neonates and adult offspring rats. After spleens in the adult offspring rats were re-stimulated with SEB in vivo or in vitro, in vivo SEB stimulation could lead to the marked decrease of splenic CD4 T cell percentage and the marked increase of splenic CD8 T cell percentage. While in vitro SEB stimulation to the cultured splenocytes markedly decreased the proliferation of the splenic lymphocytes and the CD4 T cell percentage, and had no influence on CD8 T cell percentage.
  
  
  
  The prenatal SEB exposure could alter the percentages of CD4/CD8 T cell subpopulation and the response of CD4 and CD8 T cells to the in vivo and in vitro secondary SEB stimulation in the splenocytes of adult offspring rats.
authors.affiliation: Department of Microbiology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, Anhui, 233030, People's Republic of China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhou
authors.affiliation: Editorial Board of Journal of Bengbu Medical College, Bengbu Medical College, Bengbu, Anhui, 233030, People's Republic of China.
authors.forename: Xin-Sheng
authors.initials: XS
authors.lastname: Zhang
authors.affiliation: Department of Microbiology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, Anhui, 233030, People's Republic of China.
authors.forename: Zhi-Ben
authors.initials: ZB
authors.lastname: Xu
authors.affiliation: Department of Microbiology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, Anhui, 233030, People's Republic of China.
authors.forename: Shu-Xian
authors.initials: SX
authors.lastname: Gao
authors.affiliation: Department of Microbiology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, Anhui, 233030, People's Republic of China.
authors.forename: Qing-Wei
authors.initials: QW
authors.lastname: Zheng
authors.affiliation: Department of Life Sciences, Bengbu Medical College, Bengbu, Anhui, 233030, People's Republic of China.
authors.forename: Ming-Zhu
authors.initials: MZ
authors.lastname: Xu
authors.affiliation: Scientific Research Center, Bengbu Medical College, Bengbu, Anhui, 233030, People's Republic of China.
authors.forename: Lin
authors.initials: L
authors.lastname: Shen
authors.affiliation: Huzhou University Schools of Medicine and Nursing Science, Huzhou, Zhejiang, 313000, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Yu
authors.affiliation: Department of Microbiology and Anhui Key Laboratory of Infection and Immunity, Bengbu Medical College, 2600 Dong Hai Avenue, Bengbu, Anhui, 233030, People's Republic of China. guanjc2013@126.com.
authors.forename: Jun-Chang
authors.initials: JC
authors.lastname: Guan
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001769:Blood / Q000276:immunology; D016516:CD4-CD8 Ratio; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D018414:CD8-Positive T-Lymphocytes / Q000276:immunology; D018929:Cell Culture Techniques; D049109:Cell Proliferation; D004768:Enterotoxins / Q000008:administration & dosage* / Q000097:blood* / Q000276:immunology* / Q000494:pharmacology*; D005260:Female; D005434:Flow Cytometry; D007275:Injections, Intravenous / Q000379:methods; D008214:Lymphocytes / Q000187:drug effects* / Q000276:immunology*; D011247:Pregnancy; D051381:Rats; D017207:Rats, Sprague-Dawley; D013154:Spleen / Q000276:immunology*; D013601:T-Lymphocytes / Q000382:microbiology
chemical_list: D004768:Enterotoxins; C031818:enterotoxin B, staphylococcal
keywords: Offspring; Pregnancy; Rat; Splenic lymphocyte; Staphylococcal enterotoxin B