title: High soluble CD30 levels and associated anti-HLA antibodies in patients with failed renal allografts.
abstract: BACKGROUND
  Serum soluble CD30 (sCD30), a 120-kD glycoprotein that belongs to the tumor necrosis factor receptor family, has been suggested as a marker of rejection in kidney transplant patients. The aim of this study was to evaluate the relationship between sCD30 levels and anti-HLA antibodies, and to compare sCD30 levels in patients undergoing hemodialysis (HD) with and without failed renal allografts and transplant recipients with functioning grafts.
  
  
  METHODS
  100 patients undergoing HD with failed grafts (group 1), 100 patients undergoing HD who had never undergone transplantation (group 2), and 100 kidney transplant recipients (group 3) were included in this study. Associations of serum sCD30 levels and anti-HLA antibody status were analyzed in these groups.
  
  
  RESULTS
  The sCD30 levels of group 1 and group 2 (154 ± 71 U/mL and 103 ± 55 U/mL, respectively) were significantly higher than those of the transplant recipients (group 3) (39 ± 21 U/mL) (p&lt;0.001 and p&lt;0.001). The serum sCD30 levels in group 1 (154 ± 71 U/mL) were also significantly higher than group 2 (103 ± 55 U/mL) (p&lt;0.001). Anti-HLA antibodies were detected in 81 (81%) and 5 (5%) of patients in groups 1 and 2, respectively (p&lt;0.001). When multiple regression analysis was performed to predict sCD30 levels, the independent variables in group 1 were the presence of class I anti-HLA antibodies (β = 0.295; p = 0.003) and age (β = -0.272; p = 0.005), and serum creatinine (β = 0.218; p = 0.027) and presence of class II anti-HLA antibodies (standardized β = 0.194; p = 0.046) in group 3.
  
  
  CONCLUSIONS
  Higher sCD30 levels and anti-HLA antibodies in patients undergoing HD with failed renal allografts may be related to higher inflammatory status in these patients.
authors.affiliation: Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul - Turkey.
authors.forename: Gonca E
authors.initials: GE
authors.lastname: Karahan
authors.affiliation: Division of Nephrology, Department of Internal Medicine, Istanbul Faculty of Medicine, Istanbul University, Istanbul - Turkey.
authors.forename: Yasar
authors.initials: Y
authors.lastname: Caliskan
authors.affiliation: Pediatric Bone Marrow Transplantation Unit, Medipol University, Istanbul - Turkey.
authors.forename: Kursat
authors.initials: K
authors.lastname: Ozdilli
authors.affiliation: Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul - Turkey.
authors.forename: Cigdem
authors.initials: C
authors.lastname: Kekik
authors.affiliation: Division of Transplant Surgery, Department of General Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul - Turkey.
authors.forename: Huseyin
authors.initials: H
authors.lastname: Bakkaloglu
authors.affiliation: Division of Pediatric Infectious Diseases, Department of Pediatrics, Istanbul Faculty of Medicine, Istanbul University, Istanbul - Turkey.
authors.forename: Bahar
authors.initials: B
authors.lastname: Caliskan
authors.affiliation: Pediatric Bone Marrow Transplantation Unit, Medipol University, Istanbul - Turkey.
authors.forename: Aydin
authors.initials: A
authors.lastname: Turkmen
authors.affiliation: Pediatric Bone Marrow Transplantation Unit, Medipol University, Istanbul - Turkey.
authors.forename: Mehmet S
authors.initials: MS
authors.lastname: Sever
authors.affiliation: Department of Medical Biology, Istanbul Faculty of Medicine, Istanbul University, Istanbul - Turkey.
authors.forename: Fatma S
authors.initials: FS
authors.lastname: Oguz
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000097:blood; D005260:Female; D005500:Follow-Up Studies; D006084:Graft Rejection / Q000097:blood* / Q000276:immunology; D006680:HLA Antigens / Q000276:immunology*; D006801:Humans; D007518:Isoantibodies / Q000097:blood*; D017730:Ki-1 Antigen / Q000097:blood*; D016030:Kidney Transplantation; D008297:Male; D006435:Renal Dialysis
chemical_list: D015415:Biomarkers; D006680:HLA Antigens; D007518:Isoantibodies; D017730:Ki-1 Antigen