title: Synergistic effects of acarbose and an Oroxylum indicum seed extract in streptozotocin and high-fat-diet induced prediabetic mice.
abstract: Prediabetes is defined as blood glucose levels above normal but below diabetes thresholds, and up to 70% of individuals with prediabetes will eventually develop diabetes if left untreated. Acarbose, the first FDA approved anti-prediabetes agent, has some disadvantages, such as reducing the risk of diabetes by only 36%, side effects and limited effects on complications. The aim of this study is to develop a new agent to treat prediabetes and to investigate the anti-prediabetes effects and mechanisms of acarbose and an Oroxylum indicum seed extract (OISE) in prediabetic mice. The combined drugs can reduce the dose of acarbose by 80% and reduce the risk of diabetes by 75%, which is one fold higher than acarbose monotherapy. The combined drugs showed synergistic anti-prediabetes effects and could be effective in preventing the complications of prediabetes. The combined drugs could improve glucose tolerance, improve lipid metabolism and reduce oxidative stress and tissue damage. For the mechanisms, the combined drugs can reduce synergistically postprandial hyperglycaemia by inhibiting α-glucosidase. Furthermore, baicalein in OISE was demonstrated to be a major component in reducing oxidative stress and chrysin was the primary compound that activated PPARγ.
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China.
authors.forename: Wenlong
authors.initials: W
authors.lastname: Sun
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China.
authors.forename: Yuanbin
authors.initials: Y
authors.lastname: Sang
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China.
authors.forename: Bowei
authors.initials: B
authors.lastname: Zhang
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China.
authors.forename: Xiaoxia
authors.initials: X
authors.lastname: Yu
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China.
authors.forename: Qinmin
authors.initials: Q
authors.lastname: Xu
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China.
authors.forename: Zhilong
authors.initials: Z
authors.lastname: Xiu
authors.affiliation: School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China. Electronic address: yshdong@dlut.edu.cn.
authors.forename: Yuesheng
authors.initials: Y
authors.lastname: Dong
mesh_terms: D020909:Acarbose / Q000008:administration & dosage*; D000818:Animals; D029402:Bignoniaceae; D003921:Diabetes Mellitus, Experimental / Q000188:drug therapy* / Q000378:metabolism; D059305:Diet, High-Fat / Q000009:adverse effects*; D004357:Drug Synergism; D057809:HEK293 Cells; D056945:Hep G2 Cells; D006801:Humans; D007004:Hypoglycemic Agents / Q000008:administration & dosage; D008297:Male; D051379:Mice; D024301:Plant Bark; D010936:Plant Extracts / Q000008:administration & dosage* / Q000302:isolation & purification; D011236:Prediabetic State / Q000188:drug therapy* / Q000209:etiology / Q000378:metabolism; D012639:Seeds; D013311:Streptozocin
chemical_list: D007004:Hypoglycemic Agents; D010936:Plant Extracts; D013311:Streptozocin; D020909:Acarbose
keywords: Acarbose; Anti-prediabetes; Mechanism; OISE; Synergistic