title: Antibacterial properties of nanostructured Cu-TiO2surfaces for dental implants.
abstract: The influence of copper derived TiO2surfaces (nCu-nT-TiO2) on the death of nosocomial Staphylococcus aureus (Sa) and Escherichia coli (Ec), was investigated. TiO2nanotube (nT-TiO2) arrays were fabricated by anodic oxidation of pure titanium sheets in fluorhydric solutions, leading to surface nanostructuration and creation of specific reactive sites. Copper nanocubes with a mean size of 20 nm have been synthesized and deposited on the nT-TiO2surface by pulsed electrodeposition from a copper sulphate solution. Scanning Electron Microscopy (SEM) reveals that Cu nanocubes are both inserted into the TiO2nanotubes and on the nanotube edges. X-ray Photoemission Spectroscopy (XPS) and SEM-EDX confirm the metallic nature of copper nanoparticles, covered with a thin mixed CuO-Cu2O thin layer. As the adsorption of proteins is one of the early stages of biomaterial surface interactions with body fluids before bacterial colonization, Infrared Spectroscopy (IR) in reflection-absorption mode, SEM and XPS have been used to follow the evolution of nCu-nT-TiO2surfaces when exposed to a simulated plasma solution containing Bovine Serum Albumin (BSA). Finally bacterial tests have revealed a high biocide potential of the nCu-nT-TiO2surface, which leads to the entire death of SA and EC.
authors.affiliation: UFR d'Odontologie Garancière, Université Paris Diderot Paris 7, France.
authors.forename: Jonathan
authors.initials: J
authors.lastname: Rosenbaum
authors.affiliation: Institut de Chimie et des Matériaux de Paris-Est, Université Paris-Est, CNRS, Thiais, UMR7182, France. Pdubot@icmpe.cnrs.fr.
authors.forename: Davy Louis
authors.initials: DL
authors.lastname: Versace
authors.affiliation: Institut de Chimie et des Matériaux de Paris-Est, Université Paris-Est, CNRS, Thiais, UMR7182, France. Pdubot@icmpe.cnrs.fr.
authors.forename: Samir
authors.initials: S
authors.lastname: Abbad-Andallousi
authors.affiliation: Institut de Chimie et des Matériaux de Paris-Est, Université Paris-Est, CNRS, Thiais, UMR7182, France. Pdubot@icmpe.cnrs.fr.
authors.forename: Remi
authors.initials: R
authors.lastname: Pires
authors.affiliation: UFR d'Odontologie Garancière, Université Paris Diderot Paris 7, France.
authors.forename: Christophe
authors.initials: C
authors.lastname: Azevedo
authors.affiliation: Institut de Chimie et des Matériaux de Paris-Est, Université Paris-Est, CNRS, Thiais, UMR7182, France. Pdubot@icmpe.cnrs.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Cénédese
authors.affiliation: Institut de Chimie et des Matériaux de Paris-Est, Université Paris-Est, CNRS, Thiais, UMR7182, France. Pdubot@icmpe.cnrs.fr.
authors.forename: Pierre
authors.identifier: http://orcid.org/0000-0003-4339-9683
authors.initials: P
authors.lastname: Dubot
mesh_terms: D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000494:pharmacology*; D001672:Biocompatible Materials / Q000737:chemistry / Q000494:pharmacology; D003300:Copper / Q000737:chemistry / Q000494:pharmacology*; D015921:Dental Implants / Q000382:microbiology*; D003764:Dental Materials / Q000737:chemistry / Q000494:pharmacology*; D004926:Escherichia coli / Q000187:drug effects; D004927:Escherichia coli Infections / Q000517:prevention & control; D006801:Humans; D049329:Nanostructures / Q000737:chemistry / Q000648:ultrastructure; D013203:Staphylococcal Infections / Q000517:prevention & control; D013211:Staphylococcus aureus / Q000187:drug effects; D014025:Titanium / Q000737:chemistry / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D001672:Biocompatible Materials; D015921:Dental Implants; D003764:Dental Materials; C009495:titanium dioxide; D003300:Copper; D014025:Titanium