title: MiR-365 participates in coronary atherosclerosis through regulating IL-6.
abstract: This study aims to investigate the role and mechanism of miR-365 in the coronary atherosclerosis (AS).
  
  
  
  Blood were collected from AS patients and healthy people, coronary plaque and adjacent arterial tissue were collected from AS patients. QRT-PCR method was used to detect expressions of miR-365 and IL-6 in coronary plaque, blood monocytes, and serum. Western blot was applied to detect IL-6 protein expression in coronary plaque and blood monocytes, and ELISA was used to detect IL-6 protein expression in serum.
  
  
  
  Compared with the normal group, IL-6 expression was significantly increased in coronary plaque, blood monocytes, and serum both in mRNA level and in protein level, while miR-365 expression was significantly decreased (p < 0.05).
  
  
  
  IL-6 expression was significantly up-regulated in coronary plaque, blood monocytes, and serum, whereas miR-365 expression was down-regulated. MiR-365 may regulate the pathogenesis and immune response in AS through targeting IL-6.
authors.affiliation: Department of Cardiovascular Surgery, Henan Provincial People's Hospital, Zheng Zhou, China. cfn777@126.com.
authors.forename: B
authors.initials: B
authors.lastname: Lin
authors.forename: D-G
authors.initials: DG
authors.lastname: Feng
authors.forename: F
authors.initials: F
authors.lastname: Wang
authors.forename: J-X
authors.initials: JX
authors.lastname: Wang
authors.forename: C-G
authors.initials: CG
authors.lastname: Xu
authors.forename: H
authors.initials: H
authors.lastname: Zhao
authors.forename: Z-Y
authors.initials: ZY
authors.lastname: Cheng
mesh_terms: D003324:Coronary Artery Disease / Q000097:blood*; D006801:Humans; D015850:Interleukin-6 / Q000378:metabolism*; D035683:MicroRNAs / Q000235:genetics; D058226:Plaque, Atherosclerotic
chemical_list: C508600:IL6 protein, human; D015850:Interleukin-6; C546819:MIRN365 microRNA, human; D035683:MicroRNAs