title: Principles of 60S ribosomal subunit assembly emerging from recent studies in yeast.
abstract: Ribosome biogenesis requires the intertwined processes of folding, modification, and processing of ribosomal RNA, together with binding of ribosomal proteins. In eukaryotic cells, ribosome assembly begins in the nucleolus, continues in the nucleoplasm, and is not completed until after nascent particles are exported to the cytoplasm. The efficiency and fidelity of ribosome biogenesis are facilitated by >200 assembly factors and ∼76 different small nucleolar RNAs. The pathway is driven forward by numerous remodeling events to rearrange the ribonucleoprotein architecture of pre-ribosomes. Here, we describe principles of ribosome assembly that have emerged from recent studies of biogenesis of the large ribosomal subunit in the yeast Saccharomyces cerevisiae We describe tools that have empowered investigations of ribosome biogenesis, and then summarize recent discoveries about each of the consecutive steps of subunit assembly.
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, 616 Mellon Institute, 4400 Fifth Avenue, Pittsburgh, PA 15213, U.S.A.
authors.forename: Salini
authors.initials: S
authors.lastname: Konikkat
authors.affiliation: Department of Biological Sciences, Carnegie Mellon University, 616 Mellon Institute, 4400 Fifth Avenue, Pittsburgh, PA 15213, U.S.A. jw17@andrew.cmu.edu.
authors.forename: John L
authors.initials: JL
authors.lastname: Woolford
mesh_terms: D021581:Active Transport, Cell Nucleus; D001665:Binding Sites; D002466:Cell Nucleolus / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D003593:Cytoplasm / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure; D008958:Models, Molecular; D001678:Organelle Biogenesis; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D012322:RNA Precursors / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D012335:RNA, Ribosomal / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D020537:RNA, Small Nucleolar / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D054683:Ribosome Subunits, Large, Eukaryotic / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism* / Q000648:ultrastructure
chemical_list: D012322:RNA Precursors; D012335:RNA, Ribosomal; D020537:RNA, Small Nucleolar; D012269:Ribosomal Proteins
keywords: ribonucleoproteins; ribosome assembly; ribosomes; yeast