title: Porphyromonas gingivalis activates NFκB and MAPK pathways in human oral epithelial cells.
abstract: The bacterial biofilm at the gingival margin induces a host immune reaction. In this local inflammation epithelial cells defend the host against bacterial challenge. Porphyromonas gingivalis (P. gingivalis), a keystone pathogen, infects epithelial cells. The aim of this study was to investigate the activation of signaling cascades in primary epithelial cells and oral cancer cell lines by a profiler PCR array.
  
  
  
  After infection with P. gingivalis membranes the RNA﻿ of ﻿16 to 33 of 84 key genes involved in the antibacterial immune response was up-regulated, amongst them were IKBKB (NF-κB signaling pathway), IRF5 (TLR signaling) and JUN, MAP2K4, MAPK14 and MAPK8 (MAPK pathway) in SCC-25 cells and IKBKB, IRF5, JUN, MAP2K4, MAPK14 and MAPK8 in PHGK. Statistically significant up-regulation of IKBKB (4.7 ×), MAP2K4 (4.6 ×), MAPK14 (4.2 ×) and IRF5 (9.8 ×) (p < 0.01) was demonstrated in SCC-25 cells and IKBKB (3.1 ×), MAP2K4 (4.0 ×) MAPK 14 (3.0 ×) (p < 0.05), IRF5 (3.0 ×) and JUN (7.7 ×) (p < 0.01) were up-regulated in PHGK.
  
  
  
  P. gingivalis membrane up-regulates the expression of genes involved in downstream TLR, NFκB and MAPK signaling pathways involved in the pro-inflammatory immune response in primary and malignant oral epithelial cells.
authors.affiliation: Department of Periodontology, Justus-Liebig-University of Giessen, Giessen, Germany. Sabine.E.Groeger@dentist.med.uni-giessen.de.
authors.forename: Sabine
authors.initials: S
authors.lastname: Groeger
authors.affiliation: Department of Periodontology, Justus-Liebig-University of Giessen, Giessen, Germany.
authors.forename: Fabian
authors.initials: F
authors.lastname: Jarzina
authors.affiliation: Institute for Medical Microbiology - German Center for Infection Research, DZIF Partner Site Giessen-Marburg-Langen - Justus-Liebig-University of Giessen, Giessen, Germany.
authors.forename: Eugen
authors.initials: E
authors.lastname: Domann
authors.affiliation: Department of Periodontology, Justus-Liebig-University of Giessen, Giessen, Germany.
authors.forename: Joerg
authors.initials: J
authors.lastname: Meyle
mesh_terms: D016866:Bacteroidaceae Infections / Q000276:immunology*; D018441:Biofilms; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000382:microbiology; D045744:Cell Line, Tumor; D004847:Epithelial Cells / Q000276:immunology* / Q000382:microbiology; D048049:Extracellular Signal-Regulated MAP Kinases / Q000378:metabolism; D005786:Gene Expression Regulation; D006801:Humans; D007249:Inflammation / Q000276:immunology*; D009055:Mouth / Q000473:pathology*; D009062:Mouth Neoplasms / Q000276:immunology* / Q000382:microbiology; D016328:NF-kappa B / Q000378:metabolism; D016966:Porphyromonas gingivalis / Q000502:physiology*; D015398:Signal Transduction; D051193:Toll-Like Receptors / Q000378:metabolism
chemical_list: D016328:NF-kappa B; D051193:Toll-Like Receptors; D048049:Extracellular Signal-Regulated MAP Kinases
keywords: MAPK; NF-κB; Oral cells; P. gingivalis; Signaling pathway