title: Effect of short-term zinc supplementation on zinc and selenium tissue distribution and serum antioxidant enzymes.
abstract: BACKGROUND
  A significant association between Zn and Se homeostasis exists. At the same time, data on the influence of zinc supplementation on selenium distribution in organs and tissues seem to be absent. Therefore, the primary objective of the current study is to investigate the influence of zinc asparaginate supplementation on zinc and selenium distribution and serum superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity in Wistar rats.
  
  
  METHODS
  36 rats were used in the experiment. The duration of the experiment was 7 and 14 days in the first and second series, respectively. The rats in Group I were used as the control ones. Animals in Groups II and III daily obtained zinc asparaginate (ZnA) in the doses of 5 and 15 mg/kg weight, respectively. Zinc and selenium content in liver, kidneys, heart, muscle, serum and hair was assessed using inductively coupled plasma mass spectrometry. Serum SOD and GPx activity was analysed spectrophotometrically using Randox kits.
  
  
  RESULTS
  Intragastric administration of zinc asparaginate significantly increased liver, kidney, and serum zinc content without affecting skeletal and cardiac muscle levels. Zinc supplementation also stimulated selenium retention in the rats' organs. Moreover, a significant positive correlation between zinc and selenium content was observed. Finally, zinc asparaginate treatment has been shown to modulate serum GPx but not SOD activity.
  
  
  CONCLUSIONS
  The obtained data indicate that zinc-induced increase in GPx activity may be mediated through modulation of selenium status. However, future studies are required to estimate the exact mechanisms of zinc and selenium interplay.
authors.affiliation: Federal State Scientific Institution Institute of Toxicology, Federal Medico-Biological Agency, St. Petersburg, Russia.|Russian Society of Trace Elements in Medicine, ANO Centre for Biotic Medicine, Moscow, Russia.
authors.forename: Andrey A
authors.initials: AA
authors.lastname: Skalny
authors.affiliation: Russian Society of Trace Elements in Medicine, ANO Centre for Biotic Medicine, Moscow, Russia.|Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State University, Russia.|Department of Biochemistry, Orenburg State Medical University, Russia.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Tinkov
authors.affiliation: Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscov, Russia.
authors.forename: Yulia S
authors.initials: YS
authors.lastname: Medvedeva
authors.affiliation: Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscov, Russia.
authors.forename: Irina B
authors.initials: IB
authors.lastname: Alchinova
authors.affiliation: Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscov, Russia.
authors.forename: Mikhail Y
authors.initials: MY
authors.lastname: Karganov
authors.affiliation: Russian Society of Trace Elements in Medicine, ANO Centre for Biotic Medicine, Moscow, Russia.|Laboratory of Biotechnology and Applied Bioelementology, Yaroslavl State University, Russia.|Laboratory of Physicochemical and Ecological Pathophysiology, Institute of General Pathology and Pathophysiology, Moscov, Russia.
authors.forename: Anatoly V
authors.initials: AV
authors.lastname: Skalny
authors.affiliation: Department of Biochemistry, Orenburg State Medical University, Russia.
authors.forename: Alexandr A
authors.initials: AA
authors.lastname: Nikonorov
mesh_terms: D000818:Animals; D001216:Asparagine / Q000008:administration & dosage; D003067:Coenzymes / Q000008:administration & dosage / Q000097:blood / Q000378:metabolism; D019587:Dietary Supplements; D005979:Glutathione Peroxidase / Q000097:blood* / Q000737:chemistry; D007668:Kidney / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008297:Male; D009752:Nutritional Status; D010088:Oxidoreductases / Q000097:blood*; D017207:Rats, Sprague-Dawley; D012643:Selenium / Q000378:metabolism*; D013054:Spectrophotometry, Atomic; D013482:Superoxide Dismutase / Q000097:blood; D013997:Time Factors; D014018:Tissue Distribution; D015032:Zinc / Q000008:administration & dosage* / Q000097:blood / Q000378:metabolism
chemical_list: D003067:Coenzymes; D001216:Asparagine; D010088:Oxidoreductases; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D012643:Selenium; D015032:Zinc
keywords: glutathione peroxidase; selenium; superoxide dismutase; zinc asparaginate