title: [Research of the expression of subchondral bone of Indian hedgehog with early experimental osteoarthritis induced by mechanical stress].
abstract: Objective:To investigate the the expression of subchondral bone of Indian hedgehog(Ihh) with early experimental osteoarthritis induced by mechanical stress.Methods:The animals were equally divided into two groups: experimental group(E-group,n=15) and control group(S-group,n=15). The right knee joints of E-group underwent surgery, which involved in both medial collateral ligament (MCL) transaction and medial meniscectomy, while the control group was only carried out with a sham operation.The rats were killed at 1, 2 and 4 weeks postsurgery to obtain the right knee joints. Immunostaining and immunofluorescence double staining were performed to evaluate the expression of Ihh in subchondral bone, respectively.Results:The polynuclear giant cells in the subchondral bone of E-Group expressed Ihh in their cytoplasm 1 and 2 weeks post-surgery, except for 4 week, while those of S-Group appeared negative at all three time points postsurgery. There is statistically difference between the mean density of positive area of Ihh in sections of E-group and S-group both in 1 week (E-group: 0.351+ 0.086, S-group: 0.153±0.017,P<0.05) and 2 weeks (E-group: 0.303±0.026, S-group: 0.176±0.013,P<0.05), but without statistically difference in 4 weeks (E-group: 0.092±0.033, S-group: 0.136±0.014,P>0.05) post surgery. Trap positive giant cells in subchondral bone of E-group were also found Ihh positive, which indicated expression of Ihh in osteoclast .Conclusion:Ihh maybe play an important role in pathogenesis of early experimental osteoarthritis.
authors.affiliation: Department of Orthopedics, the Fifth Affiliated Hospital of Sun Yat-sen University, Zhuhai 519000, China.
authors.forename: R K
authors.initials: RK
authors.lastname: Zhang
authors.forename: G W
authors.initials: GW
authors.lastname: Li
authors.forename: D W
authors.initials: DW
authors.lastname: Zhang
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: S Y
authors.initials: SY
authors.lastname: Feng
mesh_terms: D000818:Animals; D001842:Bone and Bones; D002358:Cartilage, Articular; D004195:Disease Models, Animal; D053823:Hedgehog Proteins; D007719:Knee Joint; D010003:Osteoarthritis; D010010:Osteoclasts; D051381:Rats; D013314:Stress, Mechanical
chemical_list: D053823:Hedgehog Proteins