title: Molecular control of Rab activity by GEFs, GAPs and GDI.
abstract: Rab proteins are the major regulators of vesicular trafficking in eukaryotic cells. Their activity can be tightly controlled within cells: Regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs), they switch between an active GTP-bound state and an inactive GDP-bound state, interacting with downstream effector proteins only in the active state. Additionally, they can bind to membranes via C-terminal prenylated cysteine residues and they can be solubilized and shuttled between membranes by chaperone-like molecules called GDP dissociation inhibitors (GDIs). In this review we give an overview of Rab proteins with a focus on the current understanding of their regulation by GEFs, GAPs and GDI.
authors.affiliation: a Department of Structural Biochemistry , Max Planck Institute of Molecular Physiology , Dortmund , Germany.
authors.forename: Matthias P
authors.identifier: 0000-0002-1529-8933
authors.initials: MP
authors.lastname: Müller
authors.affiliation: a Department of Structural Biochemistry , Max Planck Institute of Molecular Physiology , Dortmund , Germany.
authors.forename: Roger S
authors.identifier: 0000-0002-0772-0444
authors.initials: RS
authors.lastname: Goody
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D002462:Cell Membrane / Q000378:metabolism; D020690:GTPase-Activating Proteins / Q000378:metabolism*; D020730:Guanine Nucleotide Dissociation Inhibitors / Q000378:metabolism*; D020662:Guanine Nucleotide Exchange Factors / Q000378:metabolism*; D006801:Humans; D017368:Protein Prenylation; D020691:rab GTP-Binding Proteins / Q000737:chemistry / Q000378:metabolism*
chemical_list: D020690:GTPase-Activating Proteins; D020730:Guanine Nucleotide Dissociation Inhibitors; D020662:Guanine Nucleotide Exchange Factors; D020691:rab GTP-Binding Proteins
keywords: GAPS; GDI; GEFs; Rab proteins; intracellular trafficking; posttranslational modifications; vesicular transport