title: Both mechanism and age of duplications contribute to biased gene retention patterns in plants.
abstract: All extant seed plants are successful paleopolyploids, whose genomes carry duplicate genes that have survived repeated episodes of diploidization. However, the survival of gene duplicates is biased with respect to gene function and mechanism of duplication. Transcription factors, in particular, are reported to be preferentially retained following whole-genome duplications (WGDs), but disproportionately lost when duplicated by tandem events. An explanation for this pattern is provided by the Gene Balance Hypothesis (GBH), which posits that duplicates of highly connected genes are retained following WGDs to maintain optimal stoichiometry among gene products; but such connected gene duplicates are disfavored following tandem duplications.
  
  
  
  We used genomic data from 25 taxonomically diverse plant species to investigate the roles of duplication mechanism, gene function, and age of duplication in the retention of duplicate genes. Enrichment analyses were conducted to identify Gene Ontology (GO) functional categories that were overrepresented in either WGD or tandem duplications, or across ranges of divergence times. Tandem paralogs were much younger, on average, than WGD paralogs and the most frequently overrepresented GO categories were not shared between tandem and WGD paralogs. Transcription factors were overrepresented among ancient paralogs regardless of mechanism of origin or presence of a WGD. Also, in many cases, there was no bias toward transcription factor retention following recent WGDs.
  
  
  
  Both the fixation and the retention of duplicated genes in plant genomes are context-dependent events. The strong bias toward ancient transcription factor duplicates can be reconciled with the GBH if selection for optimal stoichiometry among gene products is strongest following the earliest polyploidization events and becomes increasingly relaxed as gene families expand.
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, 36570-900, Minas Gerais, Brazil.
authors.forename: Hugo V S
authors.initials: HV
authors.lastname: Rody
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
authors.forename: Gregory J
authors.initials: GJ
authors.lastname: Baute
authors.affiliation: Department of Botany, University of British Columbia, Vancouver, BC, V6T 1Z4, Canada.
authors.forename: Loren H
authors.initials: LH
authors.lastname: Rieseberg
authors.affiliation: Department of Biochemistry and Molecular Biology, Universidade Federal de Viçosa, Viçosa, 36570-900, Minas Gerais, Brazil. lorlando@ufv.br.
authors.forename: Luiz O
authors.initials: LO
authors.lastname: Oliveira
mesh_terms: D019143:Evolution, Molecular; D020131:Genes, Duplicate; D017343:Genes, Plant / Q000235:genetics*; D023281:Genomics; D010944:Plants / Q000235:genetics* / Q000378:metabolism; D012689:Sequence Homology, Nucleic Acid; D013997:Time Factors; D014157:Transcription Factors / Q000378:metabolism
chemical_list: D014157:Transcription Factors
keywords: Biased gene retention; Polyploidy; Transcription factors; Whole-genome duplication