title: Hemodynamic effects of intraoperative anesthetics administration in photothrombotic stroke model: a study using laser speckle imaging.
abstract: Previous neuroimaging studies have shown the hemodynamic effect of either preconditioning or postconditioning anesthesia in ischemic stroke model. However, the anesthetic effect in hemodynamics during and immediately after the stroke modeling surgery remains unknown due to the lack of appropriate anesthesia-free stroke model and intraoperative imaging technology. In the present study, we utilized our recently developed photothrombotic model of focal cerebral ischemia in conscious and freely moving rats, and investigated transient hemodynamic changes with or without isoflurane administration. Laser speckle imaging was applied to acquire real-time two-dimensional full-field cerebral blood flow (CBF) information throughout the surgical operations and early after.
  
  
  
  Significantly larger CBF reduction area was observed in conscious rats from 8 min immediately after the onset of stroke modeling, compared with anesthetized rats. Stroke rats without isoflurane administration also showed larger lesion volume identified by magnetic resonance imaging 3 h post occlusion (58.9%), higher neurological severity score 24 h post occlusion (28.3%), and larger infarct volume from 2,3,5-triphenyltetrazolium chloride staining 24 h post occlusion (46.9%).
  
  
  
  Our results demonstrated that the hemodynamic features were affected by anesthetics at as early as during the stroke induction. Also, our findings about the neuroprotection of intraoperative anesthetics administration bring additional insights into understanding the translational difficulty in stroke research.
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.|Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Lu
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. yaoli118@gmail.com.|Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China. yaoli118@gmail.com.
authors.forename: Yao
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Bo
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yuan
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China.
authors.forename: Hangdao
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Biomedical Engineering, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai, 200240, China. shanbao.tong@gmail.com.|Med-X Research Institute, Shanghai Jiao Tong University, 1954 Huashan Road, Shanghai, 200030, China. shanbao.tong@gmail.com.
authors.forename: Shanbao
authors.initials: S
authors.lastname: Tong
mesh_terms: D018685:Anesthetics, Inhalation / Q000008:administration & dosage*; D000818:Animals; D001921:Brain / Q000098:blood supply / Q000000981:diagnostic imaging / Q000187:drug effects* / Q000502:physiology*; D002545:Brain Ischemia / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*; D004195:Disease Models, Animal; D006439:Hemodynamics / Q000187:drug effects*; D007530:Isoflurane / Q000008:administration & dosage*; D007834:Lasers; D008279:Magnetic Resonance Imaging; D008297:Male; D061848:Optical Imaging / Q000379:methods; D017207:Rats, Sprague-Dawley; D020521:Stroke / Q000000981:diagnostic imaging / Q000473:pathology / Q000503:physiopathology*
chemical_list: D018685:Anesthetics, Inhalation; D007530:Isoflurane
keywords: Anesthetics; Hemodynamic effect; Laser speckle imaging; Photothrombotic stroke model