title: Transgenic RNAi of BmREEPa in silkworms can enhance the resistance of silkworm to Bombyxmori Nucleopolyhedrovirus.
abstract: Our previous study has identified a gene, BmREEPa, which affects BmNPV invasion in silkworm cells. In this study, we interfered with BmREEPa in silkworm larvae through transgenic technology and screened BmREEPa-RNAi silkworm strains (RP). We found the mortality in RP was lower than that in Dazao, when silkworm larvae were infected with BmNPV via oral and injection routes. And the expression level of VP39 was lower in RP than in Dazao in the group infected via injection. In the oral infection group, VP39 expression level showed significant reduction at 48 h post-infection. These results revealed that the anti-BmNPV activity was enhanced in RP, and this enhancement probably presents itself during secondary infection via BVs.
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China; College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Xiao-Long
authors.initials: XL
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Tai-Hang
authors.initials: TH
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Cai-Xia
authors.initials: CX
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Guo-Yu
authors.initials: GY
authors.lastname: Du
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.
authors.forename: Yun-Fei
authors.initials: YF
authors.lastname: Wu
authors.affiliation: AnimalScience Department, Iowa State University, USA.
authors.forename: Malavika
authors.initials: M
authors.lastname: Adur
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, China.
authors.forename: Min-Juan
authors.initials: MJ
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China. Electronic address: pmh0476@hotmail.com.
authors.forename: Min-Hui
authors.initials: MH
authors.lastname: Pan
authors.affiliation: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China. Electronic address: lucheng@swu.edu.cn.
authors.forename: Cheng
authors.initials: C
authors.lastname: Lu
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D012831:Bombyx / Q000235:genetics* / Q000821:virology*; D015870:Gene Expression; D017344:Genes, Insect; D005814:Genes, Viral; D017924:Nucleopolyhedroviruses / Q000235:genetics / Q000472:pathogenicity*; D034622:RNA Interference
keywords: Anti-BmNPV activity; BmREEPa-RNAi; Bombyx mori; Transgenosis