title: Pregabalin enhances myelin repair and attenuates glial activation in lysolecithin-induced demyelination model of rat optic chiasm.
abstract: Multiple sclerosis (MS) is an autoimmune disease in which more than 70% of patients experience visual disturbance as the earliest symptoms. Lysolecithin (LPC)-induced focal demyelination model has been developed to evaluate the effects of different therapies on myelin repair improvement. In this study, the effects of pregabalin administration on myelin repair and glial activation were investigated. Local demyelination was induced by administration of LPC (1%, 2μL) into the rat optic chiasm. Rats underwent daily injection of pregabalin (30mg/kg, i.p) or vehicle. Visual-evoked potentials (VEPs) recordings were performed for evaluating the function of optic pathway on days 3, 7, 14 and 28 post lesions. Myelin specific staining and immunostaining against GFAP and Iba1 were also carried out for assessment of myelination and glial activation respectively. Electrophysiological data indicated that pregabalin administration could significantly reduce the P1-N1 latency and increase the amplitude of VEPs waves compared to saline group. Luxol fast blue staining and immunostaining against PLP, as mature myelin marker, showed that myelin repair was improved in animals received pregabalin treatment. In addition, pregabalin effectively reduced the expression of GFAP and Iba1 as activated glial markers in optic chiasm. The present study indicates that pregabalin administration enhances myelin repair and ameliorates glial activation of optic chiasm following local injection of LPC.
authors.affiliation: Student Research Committee, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Mazandaran, Iran.
authors.forename: Danyal
authors.initials: D
authors.lastname: Daneshdoust
authors.affiliation: Student Research Committee, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Mazandaran, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Khalili-Fomeshi
authors.affiliation: Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Mazandaran, Iran; Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Mazandaran, Iran. Electronic address: m.ghasemi@mubabol.ac.ir.
authors.forename: Maryam
authors.initials: M
authors.lastname: Ghasemi-Kasman
authors.affiliation: Student Research Committee, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Mazandaran, Iran.
authors.forename: Davoud
authors.initials: D
authors.lastname: Ghorbanian
authors.affiliation: Student Research Committee, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Mazandaran, Iran.
authors.forename: Mona
authors.initials: M
authors.lastname: Hashemian
authors.affiliation: Student Research Committee, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Mazandaran, Iran.
authors.forename: Mohammad
authors.initials: M
authors.lastname: Gholami
authors.affiliation: Department of Pharmacology, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Mazandaran, Iran.
authors.forename: Aliakbar
authors.initials: A
authors.lastname: Moghadamnia
authors.affiliation: Department of Physiology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
authors.forename: Amir
authors.initials: A
authors.lastname: Shojaei
mesh_terms: D000818:Animals; D002135:Calcium-Binding Proteins / Q000378:metabolism; D020278:Demyelinating Autoimmune Diseases, CNS / Q000188:drug therapy / Q000473:pathology / Q000503:physiopathology; D005074:Evoked Potentials, Visual / Q000187:drug effects; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D008244:Lysophosphatidylcholines; D008297:Male; D008840:Microfilament Proteins / Q000378:metabolism; D009186:Myelin Sheath / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D020721:Nervous System Autoimmune Disease, Experimental / Q000188:drug therapy* / Q000473:pathology / Q000503:physiopathology; D009457:Neuroglia / Q000187:drug effects* / Q000473:pathology / Q000502:physiology; D018696:Neuroprotective Agents / Q000494:pharmacology*; D009897:Optic Chiasm / Q000187:drug effects* / Q000473:pathology / Q000503:physiopathology; D000069583:Pregabalin / Q000494:pharmacology*; D011897:Random Allocation; D017208:Rats, Wistar
chemical_list: C100745:Aif1 protein, rat; D002135:Calcium-Binding Proteins; C000614142:Gfap protein, rat; D005904:Glial Fibrillary Acidic Protein; D008244:Lysophosphatidylcholines; D008840:Microfilament Proteins; D018696:Neuroprotective Agents; D000069583:Pregabalin
keywords: glial activation; lysolecithin; multiple sclerosis; myelin repair; optic chiasm; pregabalin