title: Comparative Evaluation of Raw and Ripe Fruits of Forsythia suspensa by HPLC-ESI-MS/MS Analysis and Anti-Microbial Assay.
abstract: A multi-component quantification fingerprint based on high-performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry technique has been established for the comparative analysis of raw and ripe fruits of Forsythia suspensa originated from different provinces. Eighteen bioactive constituents including three phenylethanoid glycosides derivatives, six phenolic acids, three flavonoids, four phenylpropanoids, one fatty acid and one terpenoid were identified and quantified. Total contents of phenylethanoid glycosides, phenylpropanoids and flavonoids from raw samples were found much higher than those from ripe samples, while total content of phenolic acids showed a contrary tendency. Moreover, the anti-microbial activities were comparatively assayed for the first time using five different bacterial strains. Results revealed a positive relationship between contents of total phenolic and anti-microbial activity. The results obtained in the present study may provide useful information for future utilization of F. suspensa.
authors.affiliation: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China.|Clinical Laboratory of Integrative Medicine, The First Affiliated Hospital of Dalian Medical University, 222 Zhongshan Rd., Dalian 116011, China.
authors.forename: Jialin
authors.initials: J
authors.lastname: Qu
authors.affiliation: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China.|College of Pharmacy, Harbin University of Commerce, Harbin 150076, China.
authors.forename: Xinjia
authors.initials: X
authors.lastname: Yan
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Chunyan
authors.initials: C
authors.lastname: Li
authors.affiliation: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China.|College of Pharmacy, Harbin University of Commerce, Harbin 150076, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wen
authors.affiliation: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China.
authors.forename: Chongning
authors.initials: C
authors.lastname: Lu
authors.affiliation: College of Pharmacy, Harbin University of Commerce, Harbin 150076, China.
authors.forename: Jungang
authors.initials: J
authors.lastname: Ren
authors.affiliation: School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Peng
authors.affiliation: School of Traditional Chinese Medicine, Shenyang Pharmaceutical University, 103 Wenhua Rd., Shenyang 110016, China.
authors.forename: Shaojiang
authors.initials: S
authors.lastname: Song
mesh_terms: D000900:Anti-Bacterial Agents / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D001419:Bacteria / Q000187:drug effects; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D005419:Flavonoids; D031657:Forsythia / Q000737:chemistry*; D005638:Fruit / Q000737:chemistry*; D062385:Hydroxybenzoates; D057230:Limit of Detection; D016014:Linear Models; D050296:Microbial Viability / Q000187:drug effects; D010936:Plant Extracts / Q000032:analysis* / Q000737:chemistry / Q000494:pharmacology; D015203:Reproducibility of Results; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods
chemical_list: D000900:Anti-Bacterial Agents; D005419:Flavonoids; D062385:Hydroxybenzoates; D010936:Plant Extracts; C017616:phenolic acid