title: Optimization and Characterization of the Synthetic Secondary Chromosome synVicII inEscherichia coli.
abstract: Learning by building is one of the core ideas of synthetic biology research. Consequently, building synthetic chromosomes is the way to fully understand chromosome characteristics. The last years have seen exciting synthetic chromosome studies. We had previously introduced the synthetic secondary chromosome synVicII inEscherichia coli. It is based on the replication mechanism of the secondary chromosome inVibrio cholerae. Here, we present a detailed analysis of its genetic characteristics and a selection approach to optimize replicon stability. We probe the origin diversity of secondary chromosomes fromVibrionaceaeby construction of several new respective replicons. Finally, we present a synVicII version 2.0 with several innovations including its full compatibility with the popular modular cloning (MoClo) assembly system.
authors.affiliation: LOEWE Center for Synthetic Microbiology, SYNMIKRO, Philipps-Universität Marburg , Marburg , Germany.
authors.forename: Sonja J
authors.initials: SJ
authors.lastname: Messerschmidt
authors.affiliation: LOEWE Center for Synthetic Microbiology, SYNMIKRO, Philipps-Universität Marburg , Marburg , Germany.
authors.forename: Daniel
authors.initials: D
authors.lastname: Schindler
authors.affiliation: LOEWE Center for Synthetic Microbiology, SYNMIKRO, Philipps-Universität Marburg , Marburg , Germany.
authors.forename: Celine M
authors.initials: CM
authors.lastname: Zumkeller
authors.affiliation: LOEWE Center for Synthetic Microbiology, SYNMIKRO, Philipps-Universität Marburg , Marburg , Germany.
authors.forename: Franziska S
authors.initials: FS
authors.lastname: Kemter
authors.affiliation: LOEWE Center for Synthetic Microbiology, SYNMIKRO, Philipps-Universität Marburg , Marburg , Germany.
authors.forename: Nadine
authors.initials: N
authors.lastname: Schallopp
authors.affiliation: LOEWE Center for Synthetic Microbiology, SYNMIKRO, Philipps-Universität Marburg , Marburg , Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Waldminghaus
keywords: Vibrio cholerae; bacteria; chromosome biology; directed evolution; genetic engineering; replicon integrity; synthetic chromosomes; synthetic genomes