title: Effect of the Lipoxygenase Inhibitor Baicalein on Muscles in Ovariectomized Rats.
abstract: Sarcopenia, a loss of muscle mass accompanying osteoporosis, leads to falls and fall-related injuries. Baicalein, as a phytochemical agent, has an antioxidative and anti-inflammatory effect in muscle. In this study, sixty-one female Sprague Dawley rats were divided into five groups: four groups were ovariectomized (OVX) and one control group was nonovariectomized (NON-OVX). Eight weeks after ovariectomy, three disparate concentrations (1 mg/kg body weight (BW), 10 mg/kg BW, and 100 mg/kg BW) of baicalein were applied subcutaneously daily in three OVX groups. Mm. soleus, gastrocnemius, and longissimus were extracted; their diameter, area, relation to body, and muscle weights as well as number of capillaries per fibre were recorded. In Mm. soleus and gastrocnemius, the baicalein effect (increasing number of capillaries per fibre) was proportional to the dose applied. The fibre diameters and area under baicalein treatment were significantly greater compared to OVX and NON-OVX groups. In M. longissimus, we observed a shift to type IIa fibres. Serum creatine kinase levels were significantly lower in highest baicalein concentration group. We conclude that baicalein can stimulate angiogenesis, though not fibre type-specific, in skeletal muscle and reduce the estrogen-related loss of fibre diameter and area in the skeletal muscle in rats. Therefore, a protective effect of baicalein on muscle cells can be assumed.
authors.affiliation: Department of Trauma, Orthopedics and Reconstructive Surgery, Georg-August-University of Göttingen, Göttingen, Germany.
authors.forename: D
authors.identifier: 0000-0002-0673-3710
authors.initials: D
authors.lastname: Saul
authors.affiliation: Department of Trauma, Orthopedics and Reconstructive Surgery, Georg-August-University of Göttingen, Göttingen, Germany.
authors.forename: J H
authors.initials: JH
authors.lastname: Kling
authors.affiliation: Department of General, Visceral and Pediatric Surgery, University Medical Center Göttingen, 37075 Göttingen, Germany.
authors.forename: R L
authors.initials: RL
authors.lastname: Kosinsky
authors.affiliation: Department of Trauma, Orthopedics and Reconstructive Surgery, Georg-August-University of Göttingen, Göttingen, Germany.
authors.forename: D B
authors.initials: DB
authors.lastname: Hoffmann
authors.affiliation: Department of Trauma, Orthopedics and Reconstructive Surgery, Georg-August-University of Göttingen, Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Komrakova
authors.affiliation: Department of Animal Science, University of Göttingen, Albrecht-Thaer-Weg 3, 37075 Göttingen, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Wicke
authors.affiliation: Department of Trauma, Orthopedics and Reconstructive Surgery, Georg-August-University of Göttingen, Göttingen, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Menger
authors.affiliation: Department of Trauma, Orthopedics and Reconstructive Surgery, Georg-August-University of Göttingen, Göttingen, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Sehmisch