title: Therapeutic effect of low molecular weight chitosan containing sepia ink on ethanol-induced gastric ulcer in rats.
abstract: To evaluate the role of low molecular chitosan containing sepia ink (LMCS) in ethanol-induced (5 ml/kg) gastric ulcer in rats.
  Animals were divided into four groups (n = 12): normal group (Normal), negative control group (Con), experiment group (LMCS) and positive control Omeprazole group (OMZ). Gastric empty rate was detected in the first 7 days. Rats were sacrificed at 7, 14 and 21 day for histology and ELISA detections.
  Gastric empty was no significant differences among the groups (P > 0.05). Histological observation showed gastric mucosal LMCS treated had better healing effect. Hydroxyproline (Hyp) was significantly increased from 7 day (P < 0.05). LMCS significantly inhibited malondialdehyde (MDA) generation for lipid peroxidation from 7 day (P < 0.05). LMCS significantly promoted the activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) at the earlier stage (P < 0.05). OMZ had the similar effects above. As for myeloperoxidase (MPO), LMCS significantly decreased and restored it to normal levels from 7 day (P < 0.05), it is earlier than OMZ which is from 14 day.
  LMCS can improve gastric mucosa tissue repair, exert significant influences on oxidative and antioxidant enzyme activities and neutrophil infiltration.
authors.affiliation: Fellow Master degree, Postgraduate Program in Biomedcial Materimals, Lingnan Normal University, Round Beibu Gulf Institute for the Protection and Utilization of Marine Animals in Medicine (RBGPUM), China. Conception, design, intellectual and scientific content of the study; acquisition and interpretation of data; manuscript writing.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhang
authors.affiliation: PhD, The Key Laboratory of nutrition and feed, College of Fisheries, Guangdong Ocean University, China. Design of the study, technical procedures.
authors.forename: Kang
authors.initials: K
authors.lastname: Liu
authors.affiliation: Undergraduates, Life Science and Technology School, Lingnan Normal University, China. Acquisition of data.
authors.forename: Lei
authors.initials: L
authors.lastname: Li
authors.affiliation: Undergraduates, Life Science and Technology School, Lingnan Normal University, China. Acquisition of data.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Li
authors.affiliation: PhD, Department of Pathology and Pathological Physiology, Shanghai Medical College, Fudan University, China. Design of the study, acquisition and interpretation of data, technical procedures.
authors.forename: Xianxian
authors.initials: X
authors.lastname: Sui
authors.affiliation: Associate Professor, Postgraduate Program in Medicinal Animals, Lingnan Normal University, RBGPUM, China. Conception, design, intellectual and scientific content of the study.
authors.forename: Yinzhu
authors.initials: Y
authors.lastname: Rao
authors.affiliation: Undergraduates, Life Science and Technology School, Lingnan Normal University, China. Acquisition of data.
authors.forename: Jiahao
authors.initials: J
authors.lastname: Wu
authors.affiliation: Undergraduates, Life Science and Technology School, Lingnan Normal University, China. Acquisition of data.
authors.forename: Qiuping
authors.initials: Q
authors.lastname: Wu
mesh_terms: D000818:Animals; D000897:Anti-Ulcer Agents / Q000627:therapeutic use*; D000975:Antioxidants / Q000378:metabolism / Q000494:pharmacology*; D048271:Chitosan / Q000737:chemistry / Q000627:therapeutic use*; D004195:Disease Models, Animal; D000431:Ethanol; D005753:Gastric Mucosa / Q000187:drug effects* / Q000473:pathology; D006909:Hydroxyproline / Q000378:metabolism; D007281:Ink; D008315:Malondialdehyde / Q000378:metabolism; D008970:Molecular Weight; D011897:Random Allocation; D051381:Rats; D049833:Sepia / Q000737:chemistry*; D013276:Stomach Ulcer / Q000139:chemically induced / Q000188:drug therapy*
chemical_list: D000897:Anti-Ulcer Agents; D000975:Antioxidants; D000431:Ethanol; D008315:Malondialdehyde; D048271:Chitosan; D006909:Hydroxyproline