title: Microglial Activation by Genetically Targeted Conditional Neuronal Ablation in the Zebrafish.
abstract: In neurodegenerative diseases activation of immune cells is thought to play a major role. Microglia are the main immune cells of the central nervous system. When encountering disease related stimuli microglia adopt an activated phenotype that typically includes a rounded morphology. The exact role of microglia or other potentially infiltrating myeloid cells in different brain diseases is not fully understood. In this chapter we present techniques in zebrafish to induce degeneration of neurons, to activate the microglia, and to study activation phenotypes by immunohistochemistry and in vivo by fluorescence microscopic imaging.
authors.affiliation: Department of Clinical Genetics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Wytemaweg 80, 3015 CN, The Netherlands.
authors.forename: Nynke
authors.initials: N
authors.lastname: Oosterhof
authors.affiliation: Department of Clinical Genetics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Wytemaweg 80, 3015 CN, The Netherlands.
authors.forename: Laura E
authors.initials: LE
authors.lastname: Kuil
authors.affiliation: Department of Clinical Genetics, Erasmus MC, University Medical Center Rotterdam, Rotterdam, Wytemaweg 80, 3015 CN, The Netherlands. t.vanham@erasmusmc.nl.
authors.forename: Tjakko J
authors.initials: TJ
authors.lastname: van Ham
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D017209:Apoptosis / Q000235:genetics*; D001921:Brain / Q000378:metabolism / Q000648:ultrastructure*; D004195:Disease Models, Animal; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D007814:Larva / Q000235:genetics / Q000378:metabolism / Q000648:ultrastructure*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D017628:Microglia / Q000378:metabolism / Q000648:ultrastructure*; D008856:Microscopy, Fluorescence / Q000379:methods; D019636:Neurodegenerative Diseases / Q000235:genetics / Q000378:metabolism / Q000473:pathology; D009474:Neurons / Q000378:metabolism / Q000648:ultrastructure*; D010587:Phagocytosis; D044367:Phase Transition; D012685:Sepharose / Q000737:chemistry; D015027:Zebrafish / Q000235:genetics / Q000378:metabolism; D000097573:Red Fluorescent Protein
chemical_list: D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D012685:Sepharose; D000097573:Red Fluorescent Protein
keywords: Aging; Alzheimer’s disease; Brain; Immunohistochemistry; Live imaging; Macrophages; Microglia; Neurodegeneration; Nitroreductase; Phagocytosis; Zebrafish