title: Isolation of Hepatic Progenitor Cells From Human Liver With Cirrhosis Secondary to Biliary Atresia Using EpCAM or Thy-1 Markers.
abstract: We sought to determine whether hepatic progenitor cells can be isolated from cirrhotic liver using epithelial cell adhesion molecule (EpCAM) or Thy-1 markers. Liver tissue with cirrhosis secondary to biliary atresia (BA) was collagenase digested, and nonparenchymal cells (NPCs) were cultivated for 24 h. Noncirrhotic NPCs derived from patients with carbamyl phosphate synthetase and ornithine transcarbamylase deficiencies were used as controls. Flow cytometric analysis demonstrated that the percentages of EpCAM- and Thy-1-positive cells were significantly higher in NPC populations derived from BA liver than in those derived from control liver. Reverse transcription polymerase chain reaction analysis revealed that EpCAM-positive sorted cells expressed EpCAM, Thy-1, albumin, and CK-19, whereas Thy-1-positive sorted cells expressed Thy-1, albumin, and CK-19. These findings indicate that EpCAM- or Thy-1-positive hepatic progenitor cells can be more efficiently isolated from BA liver than from control liver and suggest that the properties of EpCAM-positive cells are somewhat different from those of Thy-1-positive cells.
authors.affiliation: Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute , Shinagawa-ku, Tokyo , Japan.
authors.forename: Taisuke
authors.initials: T
authors.lastname: Yamazaki
authors.affiliation: † Division for Advanced Medical Science, National Center for Child Health and Development , Tokyo , Japan.
authors.forename: Shin
authors.initials: S
authors.lastname: Enosawa
authors.affiliation: ‡ Division of Surgery, National Center for Child Health and Development , Tokyo , Japan.
authors.forename: Mureo
authors.initials: M
authors.lastname: Kasahara
authors.affiliation: ‡ Division of Surgery, National Center for Child Health and Development , Tokyo , Japan.
authors.forename: Akinari
authors.initials: A
authors.lastname: Fukuda
authors.affiliation: ‡ Division of Surgery, National Center for Child Health and Development , Tokyo , Japan.
authors.forename: Seisuke
authors.initials: S
authors.lastname: Sakamoto
authors.affiliation: ‡ Division of Surgery, National Center for Child Health and Development , Tokyo , Japan.
authors.forename: Takanobu
authors.initials: T
authors.lastname: Shigeta
authors.affiliation: § Department of Clinical Pathology, National Center for Child Health and Development , Tokyo , Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Nakazawa
authors.affiliation: Department of Liver Cell Biology, Kohno Clinical Medicine Research Institute , Shinagawa-ku, Tokyo , Japan.
authors.forename: Takayoshi
authors.initials: T
authors.lastname: Tokiwa
keywords: Biliary atresia; Cirrhosis; EpCAM; Flow cytometry; Hepatic progenitor cells; Thy-1