title: Effect of pterostilbene on glioma cells and related mechanisms.
abstract: Neuroglioma is the most common primary malignant tumor in neurosurgery. Due to unfavorable life quality of patients, the treatment of glioma is a major challenge in clinics. The search for effect treatment drugs thus benefits patient prognosis. As one derivative of resveratrol, pterostilbene has a wide spectrum of pharmaceutical functions, especially with the anti-tumor effects. This study thus investigated the effect of pterostilbene on neuroglioma and related mechanisms. U87 glioma cell line was divided into control, normal culture and different dosages of pterostilbene groups, which received 5 mM or 10 mM pterostilbene for 48 h. MTT assay was used to detect U87 cell proliferation, while invasion assay was employed to test the effect of pterostilbene on cell invasion, followed by flow cytometry assay for analyzing U87 cell apoptosis. Real-time PCR was used to test mRNA expression of Bcl-2 and Bax in glioma cells under the effect of pterostilbene, while Western blotting was used to detect alternation of Bcl-2 and Bax protein levels. Pterostilbene significantly inhibited proliferation and invasion abilities of glioma cells compared to those in control group (P<0.05). It can also enhance cell apoptosis, decrease mRNA and protein of Bcl-2 expression, and increase mRNA and protein expressions of Bax (P<0.05 compared to control group) in a dose-dependent manner. Pterostilbene can facilitate apoptosis of glioma cells, and inhibit their proliferation and invasion via mediating apoptotic/anti-apoptotic homeostasis.
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Liang
authors.initials: L
authors.lastname: Yu
authors.affiliation: Institute of Laboratory Animals, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhong
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Hongbin
authors.initials: H
authors.lastname: Sun
authors.affiliation: Chengdu Lilai Biotechnology Co., LTD, Tianfu Life Science Park Keyuan Nanlu High-Tech Zone, Chengdu, China.
authors.forename: Linxia
authors.initials: L
authors.lastname: Yan
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Baomin
authors.initials: B
authors.lastname: He
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Supin
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Ma
authors.affiliation: Department of Neurology, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Yang
authors.affiliation: Psychosomatic Medicine Center, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital Sichuan, Chengdu, China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Huang
keywords: Bax; Bcl-2; Glioma; cell invasion; cell proliferation; pterostilbene