title: Biocatalytic Self-Assembly Using Reversible and Irreversible Enzyme Immobilization.
abstract: Biocatalytic control of molecular self-assembly provides an effective approach for developing smart biomaterials, allowing versatile enzyme-mediated tuning of material structure and properties as well as enabling biomedical applications. We functionalized surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localization and surface release on the self-assembly process. We show how these coatings could be conveniently used to release enzymes for bulk gelation as well as to irreversibly immobilize enzymes for localizing the self-assembly to the surface. The results provide insights to the mode of action of biocatalytic self-assembly relevant to nanofabrication and enzyme-responsive materials.
authors.affiliation: WestCHEM/Department of Pure & Applied Chemistry, University of Strathclyde , 295 Cathedral Street, Glasgow G1 1XL, United Kingdom.
authors.forename: M P
authors.identifier: http://orcid.org/0000-0001-8706-4901
authors.initials: MP
authors.lastname: Conte
authors.affiliation: WestCHEM/Department of Pure & Applied Chemistry, University of Strathclyde , 295 Cathedral Street, Glasgow G1 1XL, United Kingdom.
authors.forename: K H A
authors.identifier: http://orcid.org/0000-0003-3676-9228
authors.initials: KH
authors.lastname: Lau
authors.affiliation: WestCHEM/Department of Pure & Applied Chemistry, University of Strathclyde , 295 Cathedral Street, Glasgow G1 1XL, United Kingdom.|Advanced Science Research Center (ASRC), City University of New York , 85 St Nicholas Terrace, New York, New York 10027, United States.|Department of Chemistry and Biochemistry, City University of New York-Hunter College , 695 Park Avenue, New York, New York 10065, United States.|PhD Programs in Chemistry and Biochemistry, The Graduate Center of the City University of New York , New York, New York 10016, United States.
authors.forename: R V
authors.initials: RV
authors.lastname: Ulijn
mesh_terms: D055162:Biocatalysis; D001672:Biocompatible Materials; D004800:Enzymes, Immobilized
chemical_list: D001672:Biocompatible Materials; D004800:Enzymes, Immobilized
keywords: bioinspired materials; biointerfaces; hydrogel; polydopamine; polyphenol; protein; supramolecular chemistry; surface functionalization