title: [Association between eye absent homolog 4 gene polymorphisms and occupational noise-induced hearing loss].
abstract: Objective:To identify the association between genetic polymorphisms in the eye absent homolog 4 (EYA4) gene and noise-induced hearing loss (NIHL).Method:A nested case control study was conducted based on a cohort of noise-exposed subjects. In total, 292 cases were selected from a steel factory from 6 297 subjects during Jan 1, 2006 to Dec 12, 2015,who had an average hearing threshold of more than 40 dB(A); 584 matched control subjects for each case were designated on the basis of matched criteria including same gender, age (±5 years) and duration of exposure to noise (±2 years). What's more, the control group had an average hearing threshold of less than 35 dB(A) in high frequency and less than 25 dB(A) in speech frequency. Four single nucleotide polymorphisms (SNPs) of the EYA4 gene were genotyped using a SNPscanTMmultiplex SNP genotyping kit. Hardy-Weinberg equilibrium tests were performed using a χ2test for goodness-of-fit for each SNP among the control group, and the effects of genotypes of the EYA4 gene on NIHL were analyzed by logistic regression. The haplotypes were established and their frequencies in the two groups were assessed using Haploview 4.2 and Phase 2.1 software, and interactive effects between haplotypes and cumulative noise exposure were analyzed.Results:The average age of the subjects was (40.1±8.4) years and the average number of noise-exposed working years was 20.3 (8.4, 27.3) years. The range of noise exposure levels and the cumulative noise exposure were 80.2- 98.8 dB (A) and 86.6- 111.2 dB(A) · year, respectively. After adjustment for covariates including height, blood pressure, drinking status and smoking status, in the noise intensity>85 dB (A) group, subjects carrying the rs3813346 TT genotype had a higher NIHL risk than those carrying the GG genotype, and the adjustedOR(95%CI) value was 2.12 (1.21- 3.69). In the cumulative noise exposure>98 dB (A) · year group, compared with haplotype TGC, haplotype CGT showed a protective effect in the development of NIHL, with an adjustedOR(95%CI) value of 0.60 (0.37-0.97), however, the significance of intercation between EY4 gene of noise was lost after Bonferroni correction.Conclusion:Genetic polymorphism in the EYA4 gene may be a genetic susceptibility factor for NIHL.
authors.affiliation: Department of Occupational and Environment, Peking University School of Public Health, Beijing 100191, China.
authors.forename: Q Y
authors.initials: QY
authors.lastname: Yang
authors.forename: X R
authors.initials: XR
authors.lastname: Xu
authors.forename: J
authors.initials: J
authors.lastname: Jiao
authors.forename: Y X
authors.initials: YX
authors.lastname: Zheng
authors.forename: L H
authors.initials: LH
authors.lastname: He
authors.forename: S F
authors.initials: SF
authors.lastname: Yu
authors.forename: G Z
authors.initials: GZ
authors.lastname: Gu
authors.forename: G S
authors.initials: GS
authors.lastname: Chen
authors.forename: W H
authors.initials: WH
authors.lastname: Zhou
authors.forename: H
authors.initials: H
authors.lastname: Wu
authors.forename: Y H
authors.initials: YH
authors.lastname: Li
authors.forename: H L
authors.initials: HL
authors.lastname: Zhang
authors.forename: Z R
authors.initials: ZR
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000428:Alcohol Drinking; D016022:Case-Control Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006317:Hearing Loss, Noise-Induced / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D016015:Logistic Models; D008297:Male; D008669:Metallurgy; D009623:Noise, Occupational / Q000009:adverse effects*; D016273:Occupational Exposure / Q000009:adverse effects* / Q000706:statistics & numerical data; D020641:Polymorphism, Single Nucleotide; D013232:Steel; D015534:Trans-Activators / Q000235:genetics*
chemical_list: C117736:EYA4 protein, human; D015534:Trans-Activators; D013232:Steel