title: An ultrasensitive sandwich-type electrochemical immunosensor based on the signal amplification system of double-deck gold film and thionine unite with platinum nanowire inlaid globular SBA-15 microsphere.
abstract: A novel thionine unites with platinum nanowire inlaid globular SBA-15 (Pt NWs@g-SBA-15/Thi) not only utilizes as an efficient electrical signal probe but also constitutes an amplifying system with double-deck gold film (D-Au film) have been applied to the fabrication of sandwich-type immunosensor for detecting hepatitis B surface antigen (HBs Ag). The D-Au film can accelerate the electron transfer on the electrode interface due to the tunneling effect between the two Au films and can improve the load capacity of primary antibodies (Ab1) because of the good biocompatibility. The Pt NWs@g-SBA-15/Thi with uniform globular morphology not only can effectively reduce the spatial limitation for loading the secondary antibodies (Ab2) but also can provide outstanding pore accessibility of guest species from outside and offer catalytically active sites in a large scale. Besides, the presence of Thi can well enhance the electrical conductivity of Pt NWs@g-SBA-15/Thi. With the good cooperation between D-Au film and Pt NWs@g-SBA-15/Thi, a linear relationship between current signals and the concentrations of HBs Ag was obtained in the wide range from 10 fg/mL to 100ng/mL and the detection limit of HBs Ag was 3.3 fg/mL (signal-to-noise ratio of 3). Furthermore, the designed immunosensor with excellent selectivity, reproducibility and stability shows excellent performance in detection of human serum samples and provides a promising capacity for detecting a wide range of other tumor markers in clinical application.
authors.affiliation: College of Chemistry and Molecolar Engineering, NanJing University of Technology, 211816 Nanjing, PR China; School of Chemical Engineering, Shandong University of Technology, 255049 Zibo, PR China.
authors.forename: Ping
authors.initials: P
authors.lastname: Wang
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, 255049 Zibo, PR China.
authors.forename: Mingdang
authors.initials: M
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, 255049 Zibo, PR China.
authors.forename: Fubin
authors.initials: F
authors.lastname: Pei
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, 255049 Zibo, PR China.
authors.forename: Yueyun
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, 255049 Zibo, PR China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Liu
authors.affiliation: School of Chemical Engineering, Shandong University of Technology, 255049 Zibo, PR China.
authors.forename: Yunhui
authors.initials: Y
authors.lastname: Dong
authors.affiliation: College of Chemistry and Molecolar Engineering, NanJing University of Technology, 211816 Nanjing, PR China.
authors.forename: Qingyan
authors.initials: Q
authors.lastname: Chu
authors.affiliation: College of Chemistry and Molecolar Engineering, NanJing University of Technology, 211816 Nanjing, PR China. Electronic address: zhuhj@njtech.edu.cn.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Zhu
mesh_terms: D055910:Antibodies, Immobilized / Q000737:chemistry; D015374:Biosensing Techniques / Q000379:methods; D055664:Electrochemical Techniques / Q000379:methods*; D006046:Gold / Q000737:chemistry; D006509:Hepatitis B / Q000097:blood*; D006514:Hepatitis B Surface Antigens / Q000032:analysis / Q000097:blood*; D006515:Hepatitis B virus / Q000302:isolation & purification*; D006801:Humans; D007118:Immunoassay / Q000379:methods; D057230:Limit of Detection; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D010640:Phenothiazines / Q000737:chemistry*; D010984:Platinum / Q000737:chemistry*; D015203:Reproducibility of Results; D012822:Silicon Dioxide / Q000737:chemistry
chemical_list: D055910:Antibodies, Immobilized; D006514:Hepatitis B Surface Antigens; D010640:Phenothiazines; C509969:SBA-15; D010984:Platinum; D006046:Gold; D012822:Silicon Dioxide; C009469:thionine
keywords: Double-deck gold film; Globular SBA-15; Hepatitis B surface antigen; Platinum nanowire; Sandwich-type immunosensor