title: Cancer metabolism: the volatile signature of glycolysis-in vitro model in lung cancer cells.
abstract: Discovering the volatile signature of cancer cells is an emerging approach in cancer research, as it may contribute to a fast and simple diagnosis of tumors in vivo and in vitro. One of the main contributors to such a volatile signature is hyperglycolysis, which characterizes the cancerous cell. The metabolic perturbation in cancer cells is known as the Warburg effect; glycolysis is preferred over oxidative phosphorylation (OXPHOS), even in the presence of oxygen. The precise mitochondrial alterations that underlie the increased dependence of cancer cells on aerobic glycolysis for energy generation have remained a mystery. We aimed to profile the volatile signature of the glycolysis activity in lung cancer cells. For that an in vitro model, using lung cancer cell line cultures (A549, H2030, H358, H322), was developed. The volatile signature was measured by proton transfer reaction mass spectrometry under normal conditions and glycolysis inhibition. Glycolysis inhibition and mitochondrial activity were also assessed by mitochondrial respiration capacity measurements. Cells were divided into two groups upon their glycolytic profile (PET positive and PET negative). Glycolysis blockade had a unique characteristic that was shared by all cells. Furthermore, each group had a characteristic volatile signature that enabled us to discriminate between those sub-groups of cells. In conclusion, lung cancer cells may have different subpopulations of cells upon low and high mitochondrial capacity. In both groups, glycolysis blockade induced a unique volatile signature.
authors.affiliation: Thoracic Cancer Research and Detection Center, Sheba Medical Center, Tel-Aviv University, Israel.
authors.forename: Tali
authors.initials: T
authors.lastname: Feinberg
authors.forename: Jens
authors.initials: J
authors.lastname: Herbig
authors.forename: Ingrid
authors.initials: I
authors.lastname: Kohl
authors.forename: Guy
authors.initials: G
authors.lastname: Las
authors.forename: John C
authors.initials: JC
authors.lastname: Cancilla
authors.forename: Jose S
authors.initials: JS
authors.lastname: Torrecilla
authors.forename: Maya
authors.initials: M
authors.lastname: Ilouze
authors.forename: Hossam
authors.initials: H
authors.lastname: Haick
authors.forename: Nir
authors.initials: N
authors.lastname: Peled
mesh_terms: D000143:Acids / Q000378:metabolism; D045744:Cell Line, Tumor; D005110:Extracellular Space / Q000378:metabolism; D006019:Glycolysis; D006801:Humans; D008175:Lung Neoplasms / Q000378:metabolism*; D008954:Models, Biological; D010101:Oxygen Consumption; D055549:Volatile Organic Compounds / Q000378:metabolism*
chemical_list: D000143:Acids; D055549:Volatile Organic Compounds