title: Population dynamics, antibiotics resistance and biofilm formation of Aeromonas and Vibrio species isolated from aquatic sources in Northern Malaysia.
abstract: This study aimed to compare population dynamics, antibiotic resistance and biofilm formation of Aeromonas and Vibrio species from seawater and sediment collected from Northern Malaysia. Isolates with different colony morphology were characterized using both biochemical and molecular methods before testing for antibiotic resistance and biofilm formation. Results obtained from this study showed that in Kedah, the population of Aeromonas isolated from sediment was highest in Pantai Merdeka (8.22 log CFU/ml), Pulau Bunting recorded the highest population of Aeromonas from sediment (8.43 log CFU/g). It was observed that Vibrio species isolated from seawater and sediment were highest in Kuala Sanglang (9.21 log CFU/ml). In Kuala Perlis, the population of Aeromonas isolated from seawater was highest in Jeti (7.94 log CFU/ml). Highest population of Aeromonas from sediment was recorded in Kampong Tanah Baru (7.99 log CFU/g). It was observed that Vibrio species isolated from seawater was highest in Padang Benta (8.42 log CFU/g) while Jeti Kuala Perlis had highest population of Vibrio isolated from sediment. It was observed that location does not influence population of Aeromonas. The results of the independent t - test revealed that there was no significant relationship between location and population of Vibrio (df = 10, t = 1.144, p > 0.05). The occurrence of biofilm formation and prevalence of antibiotic resistant Aeromonas and Vibrio species in seawater and sediment pose danger to human and aquatic animals' health.
authors.affiliation: Aquatic Microbiology Laboratory, School of Biotechnology and Biosciences, Faculty of Science and Technology, National University of Malaysia, Malaysia. Electronic address: oluodeyemi@gmail.com.
authors.forename: Olumide A
authors.initials: OA
authors.lastname: Odeyemi
authors.affiliation: Aquatic Microbiology Laboratory, School of Biotechnology and Biosciences, Faculty of Science and Technology, National University of Malaysia, Malaysia. Electronic address: asmat@ukm.edu.my.
authors.forename: Asmat
authors.initials: A
authors.lastname: Ahmad
mesh_terms: D000333:Aeromonas / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D018441:Biofilms; D024881:Drug Resistance, Bacterial; D005843:Geography; D019015:Geologic Sediments / Q000382:microbiology; D016905:Gram-Negative Bacterial Infections / Q000453:epidemiology* / Q000382:microbiology*; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012623:Seawater / Q000382:microbiology; D014733:Vibrio / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D014735:Vibrio Infections / Q000453:epidemiology / Q000382:microbiology; D014871:Water Microbiology
chemical_list: D012336:RNA, Ribosomal, 16S
keywords: Aeromonas species; Aquatic sources; Biofilm formation; Vibrio spp.