title: GDF8 inhibits bone formation and promotes bone resorption in mice.
abstract: Growth Differentiation Factor 8 (GDF8), also called myostatin, is a member of the transforming growth factor (TGF)-β super-family. As a negative regulator of skeletal muscle growth, GDF8 is also associated with bone metabolism. However, the function of GDF8 in bone metabolism is not fully understood. Our study aimed to investigate the role of GDF8 in bone metabolism, both in vitro and in vivo. Our results showed that GDF8 had a negative regulatory effect on primary mouse osteoblasts, and promoted receptor activator of nuclear factor κB ligand (RANKL)-induced osteoclastogenesis in vitro. Intraperitoneal injection of recombinant GDF8 repressed bone formation and accelerated bone resorption in mice. Furthermore, treatment of aged mice with a GDF8 neutralizing antibody stimulated new bone formation and prevented bone resorption. Thus, our study showed that GDF8 plays a significant regulatory role in bone formation and bone resorption, thus providing a potential therapeutic pathway for osteoporosis.
authors.affiliation: Department of Endocrinology, The Xiangya Hospital of Central South University, Changsha, Hunan, China.|Department of Gerontology, The Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Yu-Si
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Endocrinology, The Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Guo
authors.affiliation: Department of Endocrinology, The Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Li-Juan
authors.initials: LJ
authors.lastname: Guo
authors.affiliation: Institute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Liu
authors.affiliation: Institute of Endocrinology and Metabolism, The Second Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Xian-Ping
authors.initials: XP
authors.lastname: Wu
authors.affiliation: Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Zhang-Yuan
authors.identifier: http://orcid.org/0000-0002-3880-5600
authors.initials: ZY
authors.lastname: Lin
authors.affiliation: Department of Orthopedics, The Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Hong-Bo
authors.initials: HB
authors.lastname: He
authors.affiliation: Department of Endocrinology, The Xiangya Hospital of Central South University, Changsha, Hunan, China.
authors.forename: Tie-Jian
authors.initials: TJ
authors.lastname: Jiang
mesh_terms: D000818:Animals; D001862:Bone Resorption / Q000473:pathology / Q000503:physiopathology*; D002113:Calcification, Physiologic / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D005260:Female; D051379:Mice; D008810:Mice, Inbred C57BL; D055435:Myostatin / Q000378:metabolism*; D010006:Osteoblasts / Q000187:drug effects / Q000473:pathology; D010012:Osteogenesis / Q000187:drug effects; D053245:RANK Ligand / Q000494:pharmacology
chemical_list: D055435:Myostatin; D053245:RANK Ligand
keywords: GDF8; neutralizing antibody; osteoblast; osteoclastogenesis; osteoporosis