title: Protective effects of melatonin on the activity of SOD, CAT, GSH-Px and GSH content in organs of mice after administration of SNP.
abstract: Sodium nitroprusside (SNP) is an antihypertensive drug with proven dose-dependent toxic
  effects attributed mainly to the production of cyanide but also excesive nitric oxide (NO) and derived
  reactive species. The present study evaluated whether melatonin administration would have time-dependent
  protective effect against SNP−induced toxicity. Male Swiss mice were used in this study.
  Control mice were treated with 0.9% NaCl; the second group was injected with 10 mg melatonin
  (MEL)/kg body weight (b.w.); the third group was given SNP at the dose of 3,6 mg/kg b.w.; the fourth
  group received both MEL and SNP at the same doses. In homogenates of brain, liver and kidneys,
  activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) were
  estimated after 3, 6 and 24 h of drugs administration. The concentration of reduced glutathione
  (GSH) was also evaluated in the blood, brain, liver and kidneys of mice at the same time intervals.
  In animals receiving MEL, the highest levels of GSH were observed in all the organs as compared to
  the control after 3, 6 h. Meanwhile, SNP decreased GSH concentration in the blood, brain, liver and
  kidneys in all time intervals. Administration of MEL in combination with SNP increased the GSH
  levels in all organs, as compared to the administration of SNP alone; this effect was observed after 3,
  6 and 24 h. The activity of SOD, CAT and GSH-Px in the MEL-treated group increased after 3 h in
  all the organs, while in liver and kidney the increase was also observed after 6 h. Conversely, the SNP
  intoxication caused a decrease of the activity of enzymes in the tested organs in all intervals, while
  administration of MEL + SNP resulted in increased activities of SOD, CAT and GSH-Px in all the
  organs after 3 h and 6 h. The investigation carried out in the present study provide new data to add
  to the study of antioxidant properties of MEL and SNP-induced oxidative stress with regard to time-dependent
  properties in different types of tissues.
authors.affiliation: Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow, Podbrzezie 3, 31-054 Cracow, Poland.
authors.forename: Zofia
authors.initials: Z
authors.lastname: Goc
authors.affiliation: Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow, Podbrzezie 3, 31-054 Cracow, Poland.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Szaroma
authors.affiliation: Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow, Podbrzezie 3, 31-054 Cracow, Poland.
authors.forename: Edyta
authors.initials: E
authors.lastname: Kapusta
authors.affiliation: Department of Animal Physiology and Toxicology, Institute of Biology, Pedagogical University of Cracow, Podbrzezie 3, 31-054 Cracow, Poland.
authors.forename: Karol
authors.initials: K
authors.lastname: Dziubek
mesh_terms: D000818:Animals; D000959:Antihypertensive Agents / Q000009:adverse effects*; D000975:Antioxidants / Q000378:metabolism / Q000627:therapeutic use*; D002374:Catalase / Q000378:metabolism; D004353:Drug Evaluation, Preclinical; D005978:Glutathione / Q000378:metabolism; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D008550:Melatonin / Q000627:therapeutic use*; D051379:Mice; D009599:Nitroprusside / Q000009:adverse effects*; D018384:Oxidative Stress / Q000187:drug effects*; D013482:Superoxide Dismutase / Q000378:metabolism
chemical_list: D000959:Antihypertensive Agents; D000975:Antioxidants; D009599:Nitroprusside; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D005978:Glutathione; D008550:Melatonin
keywords: catalase; glutathione peroxidase; melatonin; reduced glutathione; sodium nitroprusside; superoxide dismutase