title: Microneedle-Assisted Skin Permeation by Nontoxic Bioengineerable Gas Vesicle Nanoparticles.
abstract: Gas vesicle nanoparticles (GVNPs) are hollow, buoyant protein organelles produced by the extremophilic microbe Halobacterium sp. NRC-1 and are being developed as bioengineerable and biocompatible antigen and drug-delivery systems (DDS). Dynamic light scattering measurements of purified GVNP suspensions showed a mean diameter of 245 nm. In vitro diffusion studies using Yucatan miniature pig skin showed GVNP permeation to be enhanced after MN-treatment compared to untreated skin. GVNPs were found to be nontoxic to mammalian cells (human kidney and rat mycocardial myoblasts). These findings support the use of GVNPs as DDS for intradermal/transdermal permeation of protein- and peptide-based drugs.
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland , Baltimore, Maryland 21201, United States.
authors.forename: Abhay U
authors.initials: AU
authors.lastname: Andar
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, and Institute of Marine and Environmental Technology, University System of Maryland , Baltimore, Maryland 21202, United States.
authors.forename: Ram
authors.initials: R
authors.lastname: Karan
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, and Institute of Marine and Environmental Technology, University System of Maryland , Baltimore, Maryland 21202, United States.|Yale Gordon College of Arts and Sciences, University of Baltimore , Baltimore, Maryland 21201, United States.
authors.forename: Wolf T
authors.initials: WT
authors.lastname: Pecher
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, and Institute of Marine and Environmental Technology, University System of Maryland , Baltimore, Maryland 21202, United States.
authors.forename: Priya
authors.initials: P
authors.lastname: DasSarma
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland , Baltimore, Maryland 21201, United States.
authors.forename: William D
authors.initials: WD
authors.lastname: Hedrich
authors.affiliation: Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland , Baltimore, Maryland 21201, United States.
authors.forename: Audra L
authors.identifier: 0000-0001-7316-969X
authors.initials: AL
authors.lastname: Stinchcomb
authors.affiliation: Department of Microbiology and Immunology, School of Medicine, and Institute of Marine and Environmental Technology, University System of Maryland , Baltimore, Maryland 21202, United States.
authors.forename: Shiladitya
authors.initials: S
authors.lastname: DasSarma
mesh_terms: D000279:Administration, Cutaneous; D000818:Animals; D004058:Diffusion; D004337:Drug Carriers / Q000008:administration & dosage*; D016503:Drug Delivery Systems / Q000379:methods; D000067493:Dynamic Light Scattering / Q000379:methods; D005740:Gases / Q000008:administration & dosage*; D006801:Humans; D008322:Mammals / Q000378:metabolism; D053758:Nanoparticles / Q000008:administration & dosage*; D009339:Needles; D010539:Permeability; D004364:Pharmaceutical Preparations / Q000008:administration & dosage*; D051381:Rats; D012867:Skin / Q000378:metabolism*; D012869:Skin Absorption / Q000502:physiology; D013552:Swine
chemical_list: D004337:Drug Carriers; D005740:Gases; D004364:Pharmaceutical Preparations
keywords: Yucatan minipig skin; gas vesicle nanoparticles; halobacterium; in vitro; microneedles