title: Interaction between in vivo bioluminescence and extracellular electron transfer in Shewanella woodyi via charge and discharge.
abstract: Extracellular electron transfer (EET) and bioluminescence are both important for microbial growth and metabolism, but the mechanism of interaction between EET and bioluminescence is poorly understood. Herein, we demonstrate an exclusively respiratory luminous bacterium, Shewanella woodyi, which possesses EET ability and electron communication at the interface of S. woodyi and solid substrates via charge and discharge methods. Using an electro-chemiluminescence apparatus, our results confirmed that the FMN/FMNH2content and the redox status of cytochrome c conjointly regulated the bioluminescence intensity when the potential of an indium-tin oxide electrode was changed. More importantly, this work revealed that there is an interaction between the redox reaction of single cells and bioluminescence of group communication via the EET pathway.
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. yxjiang@xmu.edu.cn.
authors.forename: Xiaochun
authors.initials: X
authors.lastname: Tian
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China. fzhao@iue.ac.cn.
authors.forename: Feng
authors.identifier: http://orcid.org/0000-0002-1525-042X
authors.initials: F
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. yxjiang@xmu.edu.cn.
authors.forename: Lexing
authors.initials: L
authors.lastname: You
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. yxjiang@xmu.edu.cn.
authors.forename: Xuee
authors.initials: X
authors.lastname: Wu
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China. fzhao@iue.ac.cn.
authors.forename: Zhiyong
authors.initials: Z
authors.lastname: Zheng
authors.affiliation: CAS Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361021, China. fzhao@iue.ac.cn.
authors.forename: Ranran
authors.initials: R
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. yxjiang@xmu.edu.cn.
authors.forename: Yanxia
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Physical Chemistry of Solid Surfaces, Department of Chemistry, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China. yxjiang@xmu.edu.cn.
authors.forename: Shigang
authors.initials: S
authors.lastname: Sun
mesh_terms: D002460:Cell Line; D045304:Cytochromes c / Q000378:metabolism; D055664:Electrochemical Techniques / Q000379:methods*; D004579:Electron Transport / Q000502:physiology; D005486:Flavin Mononucleotide / Q000378:metabolism; D006873:Hydroquinones / Q000378:metabolism; D008163:Luminescent Measurements / Q000379:methods*; D010084:Oxidation-Reduction; D020592:Shewanella / Q000378:metabolism*; D017971:Tin Compounds
chemical_list: D006873:Hydroquinones; D017971:Tin Compounds; C540087:flavin mononucleotide hydroquinone; C109984:indium tin oxide; D005486:Flavin Mononucleotide; D045304:Cytochromes c