title: Rice bran polysaccharides and oligosaccharides modified by Grifola frondosa fermentation: Antioxidant activities and effects on the production of NO.
abstract: Rice bran polysaccharides (RBPSs) are valuable compounds with many biological activities. In this work, a fungus called Grifola frondosa, was selected to ferment defatted rice bran water extracts and modify the RBPSs, which were then isolated by ethanol precipitation and deproteinization. GC analysis of fermented products suggested they are composed of glucose, arabinose, galactose, mannose, and xylose at a molar ratio of 9:5:8:2:5, which was 32:4:6:2:5 before fermentation. HPLC analysis revealed that the molecular weight of unfermented RBPS was distributed mainly from 103to 104Da, and it changed to 102to 103Da after fermentation. Antioxidant activities and effects on the production of NO were analyzed and it indicated that the scavenging ratios of hydroxyl and DPPH radicals by the fermented products were significantly enhanced compared to the unfermented ones, and also the products fermented for 9days exhibited two-way adjusting effects on the production of NO in macrophages.
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, PR China.
authors.forename: Qian
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, PR China; School of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Xiujuan
authors.initials: X
authors.lastname: Cao
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, PR China.
authors.forename: Xuhui
authors.initials: X
authors.lastname: Zhuang
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Han
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, PR China.
authors.forename: Weiqun
authors.initials: W
authors.lastname: Guo
authors.affiliation: School of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, PR China.
authors.forename: Jian
authors.initials: J
authors.lastname: Xiong
authors.affiliation: Academy of State Administration of Grain, Beijing 100037, PR China. Electronic address: zxl@chinagrain.org.
authors.forename: Xiaolin
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000737:chemistry; D002460:Cell Line; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004305:Dose-Response Relationship, Drug; D005285:Fermentation / Q000187:drug effects / Q000502:physiology*; D016166:Free Radical Scavengers / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D048691:Grifola / Q000737:chemistry / Q000378:metabolism*; D051379:Mice; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis*; D009844:Oligosaccharides / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology; D012275:Oryza / Q000737:chemistry / Q000378:metabolism*; D010084:Oxidation-Reduction / Q000187:drug effects; D010936:Plant Extracts / Q000302:isolation & purification / Q000378:metabolism / Q000494:pharmacology; D011134:Polysaccharides / Q000302:isolation & purification / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D000975:Antioxidants; D016166:Free Radical Scavengers; D009844:Oligosaccharides; D010936:Plant Extracts; D011134:Polysaccharides; D009569:Nitric Oxide
keywords: Antioxidant activity; Fermentation; Grifola frondosa; NO; Oligosaccharides; Rice bran polysaccharides