title: Intrinsic blinking of red fluorescent proteins for super-resolution microscopy.
abstract: Single-molecule localization microscopy relies on either controllable photoswitching of fluorescent probes or their robust blinking. We have found that blinking of monomeric red fluorescent proteins TagRFP, TagRFP-T, and FusionRed occurs at moderate illumination power and matches well with camera acquisition speed. It allows for super-resolution image reconstruction of densely labelled structures in live cells using various algorithms.
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Natalia V
authors.identifier: http://orcid.org/0000-0002-2742-372X
authors.initials: NV
authors.lastname: Klementieva
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Anton I
authors.initials: AI
authors.lastname: Pavlikov
authors.affiliation: Institute of Applied Physics, Nizhny Novgorod, Russia.
authors.forename: Alexander A
authors.initials: AA
authors.lastname: Moiseev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
authors.forename: Nina G
authors.initials: NG
authors.lastname: Bozhanova
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
authors.forename: Natalie M
authors.initials: NM
authors.lastname: Mishina
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru and Pirogov Russian National Research Medical University, Moscow, Russia.
authors.forename: Sergey A
authors.initials: SA
authors.lastname: Lukyanov
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Zagaynova
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
authors.forename: Konstantin A
authors.initials: KA
authors.lastname: Lukyanov
authors.affiliation: Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia and Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia. mishin@ibch.ru.
authors.forename: Alexander S
authors.identifier: http://orcid.org/0000-0002-4935-7030
authors.initials: AS
authors.lastname: Mishin
mesh_terms: D000465:Algorithms; D006367:HeLa Cells; D006801:Humans; D008164:Luminescent Proteins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein