title: Dose-rate plays a significant role in synchrotron radiation X-ray-induced damage of rodent testes.
abstract: Synchrotron radiation (SR) X-ray has significant potential for applications in medical imaging and cancer treatment. However, the mechanisms underlying SR X-ray-induced tissue damage remain unclear. Previous studies on regular X-ray-induced tissue damage have suggested that dose-rate could affect radiation damage. Because SR X-ray has exceedingly high dose-rate compared to regular X-ray, it remains to be determined if dose-rate may affect SR X-ray-induced tissue damage. We used rodent testes as a model to investigate the role of dose-rate in SR X-ray-induced tissue damage. One day after SR X-ray irradiation, we determined the effects of the irradiation of the same dosage at two different dose-rates, 0.11 Gy/s and 1.1 Gy/s, on TUNEL signals, caspase-3 activation and DNA double-strand breaks (DSBs) of the testes. Compared to those produced by the irradiation at 0.11 Gy/s, irradiation at 1.1 Gy/s produced higher levels of DSBs, TUNEL signals, and caspase-3 activation in the testes. Our study has provided the first evidence suggesting that dose-rate could be a significant factor in SR X-ray-induced tissue damage, which may establish a valuable base for utilizing this factor to manipulate the tissue damage in SR X-ray-based medical applications.
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Heyu
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Ban
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Caixia
authors.initials: C
authors.lastname: Wang
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Wei
authors.initials: W
authors.lastname: Cao
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Jie
authors.initials: J
authors.lastname: Zhang
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Yingxin
authors.initials: Y
authors.lastname: Ma
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Yunyi
authors.initials: Y
authors.lastname: Hong
authors.affiliation: Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Fudan University Cancer Hospital Shanghai, P. R. China.
authors.forename: Shen
authors.initials: S
authors.lastname: Fu
authors.affiliation: Department of Civil Engineering, School of Naval Architecture, Ocean & Civil Engineering, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Fan
authors.initials: F
authors.lastname: Wu
authors.affiliation: School of Biomedical Engineering and Med-X Research Institute, Shanghai Jiao Tong University Shanghai, P. R. China.
authors.forename: Weihai
authors.initials: W
authors.lastname: Ying
keywords: Synchrotron radiation X-ray; dose-rate; rodent testes; tissue damage