title: Resolvase OsGEN1 Mediates DNA Repair by Homologous Recombination.
abstract: Yen1/GEN1 are canonical Holliday junction resolvases that belong to the RAD2/XPG family. In eukaryotes, such as budding yeast, mice, worms, and humans, Yen1/GEN1 work together with Mus81-Mms4/MUS81-EME1 and Slx1-Slx4/SLX1-SLX4 in DNA repair by homologous recombination to maintain genome stability. In plants, the biological function of Yen1/GEN1 remains largely unclear. In this study, we characterized the loss of function mutants of OsGEN1 and OsSEND1, a pair of paralogs of Yen1/GEN1 in rice (Oryza sativa). We first investigated the role of OsGEN1 during meiosis and found a reduction in chiasma frequency by ∼6% in osgen1 mutants, compared to the wild type, suggesting a possible involvement of OsGEN1 in the formation of crossovers. Postmeiosis, OsGEN1 foci were detected in wild-type microspore nuclei, but not in the osgen1 mutant concomitant with an increase in double-strand breaks. Persistent double-strand breaks led to programmed cell death of the male gametes and complete male sterility. In contrast, depletion of OsSEND1 had no effects on plant development and did not enhance osgen1 defects. Our results indicate that OsGEN1 is essential for homologous recombinational DNA repair at two stages of microsporogenesis in rice.
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China (C.W., Y.H., D.Z., W.L.).|Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom (J.D.H.).|State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China (P.L.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Chong
authors.identifier: 0000-0001-9433-0112
authors.initials: C
authors.lastname: Wang
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China (C.W., Y.H., D.Z., W.L.).|Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom (J.D.H.).|State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China (P.L.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: James D
authors.identifier: 0000-0001-6027-8678
authors.initials: JD
authors.lastname: Higgins
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China (C.W., Y.H., D.Z., W.L.).|Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom (J.D.H.).|State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China (P.L.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Yi
authors.identifier: 0000-0002-5218-541X
authors.initials: Y
authors.lastname: He
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China (C.W., Y.H., D.Z., W.L.).|Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom (J.D.H.).|State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China (P.L.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Pingli
authors.initials: P
authors.lastname: Lu
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China (C.W., Y.H., D.Z., W.L.).|Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom (J.D.H.).|State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China (P.L.); and.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.).
authors.forename: Dabing
authors.identifier: 0000-0002-1764-2929
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Joint International Research Laboratory of Metabolic and Developmental Sciences, Shanghai Jiao Tong University-University of Adelaide Joint Centre for Agriculture and Health, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 20040, China (C.W., Y.H., D.Z., W.L.); wqliang@sjtu.edu.cn.|Department of Genetics, University of Leicester, Leicester LE1 7RH, United Kingdom (J.D.H.); wqliang@sjtu.edu.cn.|State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, Institute of Plant Biology, School of Life Sciences, Fudan University, Shanghai 200433, China (P.L.); and wqliang@sjtu.edu.cn.|School of Agriculture, Food and Wine, University of Adelaide, Urrbrae, South Australia 5064, Australia (D.Z.) wqliang@sjtu.edu.cn.
authors.forename: Wanqi
authors.identifier: 0000-0002-9938-5793
authors.initials: W
authors.lastname: Liang
mesh_terms: D032461:Chromosomes, Plant / Q000235:genetics / Q000378:metabolism; D004260:DNA Repair / Q000502:physiology*; D059765:Homologous Recombination; D008540:Meiosis; D009154:Mutation; D012275:Oryza / Q000235:genetics* / Q000254:growth & development; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D011058:Pollen / Q000235:genetics; D011994:Recombinant Proteins / Q000235:genetics / Q000378:metabolism; D045522:Recombinases / Q000235:genetics / Q000378:metabolism*; D013573:Synaptonemal Complex / Q000235:genetics / Q000378:metabolism
chemical_list: D010940:Plant Proteins; D011994:Recombinant Proteins; D045522:Recombinases