title: UUAT1 Is a Golgi-Localized UDP-Uronic Acid Transporter That Modulates the Polysaccharide Composition of Arabidopsis Seed Mucilage.
abstract: UDP-glucuronic acid (UDP-GlcA) is the precursor of many plant cell wall polysaccharides and is required for production of seed mucilage. Following synthesis in the cytosol, it is transported into the lumen of the Golgi apparatus, where it is converted to UDP-galacturonic acid (UDP-GalA), UDP-arabinose, and UDP-xylose. To identify the Golgi-localized UDP-GlcA transporter, we screenedArabidopsis thalianamutants in genes coding for putative nucleotide sugar transporters for altered seed mucilage, a structure rich in the GalA-containing polysaccharide rhamnogalacturonan I. As a result, we identifiedUUAT1, which encodes a Golgi-localized protein that transports UDP-GlcA and UDP-GalA in vitro. The seed coat ofuuat1mutants had less GalA, rhamnose, and xylose in the soluble mucilage, and the distal cell walls had decreased arabinan content. Cell walls of other organs and cells had lower arabinose levels in roots and pollen tubes, but no differences were observed in GalA or xylose contents. Furthermore, the GlcA content of glucuronoxylan in the stem was not affected in the mutant. Interestingly, the degree of homogalacturonan methylation increased inuuat1These results suggest that this UDP-GlcA transporter plays a key role defining the seed mucilage sugar composition and that its absence produces pleiotropic effects in this component of the plant extracellular matrix.
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Susana
authors.initials: S
authors.lastname: Saez-Aguayo
authors.affiliation: ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia.
authors.forename: Carsten
authors.initials: C
authors.lastname: Rautengarten
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Henry
authors.initials: H
authors.lastname: Temple
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Dayan
authors.initials: D
authors.lastname: Sanhueza
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Troy
authors.initials: T
authors.lastname: Ejsmentewicz
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Omar
authors.initials: O
authors.lastname: Sandoval-Ibañez
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Daniela
authors.identifier: 0000-0001-7703-5613
authors.initials: D
authors.lastname: Doñas
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile.
authors.forename: Juan Pablo
authors.initials: JP
authors.lastname: Parra-Rojas
authors.affiliation: ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia.
authors.forename: Berit
authors.initials: B
authors.lastname: Ebert
authors.affiliation: Normandy University, UniRouen, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, EA4358, IRIB, VASI, France.
authors.forename: Arnaud
authors.identifier: 0000-0003-4442-1731
authors.initials: A
authors.lastname: Lehner
authors.affiliation: Normandy University, UniRouen, Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale, EA4358, IRIB, VASI, France.
authors.forename: Jean-Claude
authors.identifier: 0000-0001-8717-0034
authors.initials: JC
authors.lastname: Mollet
authors.affiliation: Department of Biochemistry, University of Cambridge, Cambridge CB2 1QW, United Kingdom.
authors.forename: Paul
authors.identifier: 0000-0001-9270-6286
authors.initials: P
authors.lastname: Dupree
authors.affiliation: Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702.|Department of Plant and Microbial Biology, University of California, Berkeley, California 94720.
authors.forename: Henrik V
authors.identifier: 0000-0002-6702-3560
authors.initials: HV
authors.lastname: Scheller
authors.affiliation: ARC Centre of Excellence in Plant Cell Walls, School of BioSciences, The University of Melbourne, Victoria 3010, Australia.|Joint BioEnergy Institute and Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, California 94702.
authors.forename: Joshua L
authors.identifier: 0000-0002-2080-3826
authors.initials: JL
authors.lastname: Heazlewood
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile aorellana@unab.cl francisca.reyes.marquez@gmail.com.
authors.forename: Francisca C
authors.initials: FC
authors.lastname: Reyes
authors.affiliation: Centro de Biotecnología Vegetal, FONDAP Center for Genome Regulation, Facultad de Ciencias Biológicas, Universidad Andrés Bello, Santiago, Chile aorellana@unab.cl francisca.reyes.marquez@gmail.com.
authors.forename: Ariel
authors.identifier: 0000-0002-9243-808X
authors.initials: A
authors.lastname: Orellana
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D002473:Cell Wall / Q000235:genetics / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D006056:Golgi Apparatus / Q000378:metabolism*; D015151:Immunoblotting; D018613:Microscopy, Confocal; D009154:Mutation; D033704:Nucleotide Transport Proteins / Q000235:genetics / Q000378:metabolism*; D010368:Pectins / Q000378:metabolism; D030821:Plants, Genetically Modified; D011134:Polysaccharides / Q000378:metabolism*; D012639:Seeds / Q000235:genetics / Q000378:metabolism*; D014539:Uridine Diphosphate Sugars / Q000378:metabolism
chemical_list: D029681:Arabidopsis Proteins; D033704:Nucleotide Transport Proteins; D011134:Polysaccharides; C000624952:UUAT1 protein, Arabidopsis; D014539:Uridine Diphosphate Sugars; C042491:rhamnogalacturonan I; C050306:UDP-galacturonic acid; D010368:Pectins