title: Anti-Diabetic Effects of Jiang Tang Xiao Ke Granule via PI3K/Akt Signalling Pathway in Type 2 Diabetes KKAy Mice.
abstract: Jiang Tang Xiao Ke (JTXK) granule, a Chinese herbal formula, has been used clinically to treat type 2 diabetes (T2DM) for decades. Our previous studies showed that JTXK granule exhibited anti-diabetic and anti-oxidative functions in experimental diabetic rats induced by a high fat diet and streptozotocin. However, the underlying mechanisms remain poorly understood. Herein, we aimed to investigate the therapeutic effect of JTXK granule on T2DM KKAy mice and the possible associations with skeletal muscle in the current study. Our results showed that JTXK granule significantly reduced food intake and body weight in T2DM KKAy mice. JTXK granule treatment also decreased the blood glucose and HbA1c levels and increased the insulin sensitivity in a time-dependent manner. Additionally, it ameliorated hyperlipidaemia and induced a lower free fatty acid level, displaying an effect on disorders of lipid metabolism. JTXK granule significantly increased the expression of insulin receptor substrate-1 (IRS-1), phosphoinositide 3-kinase (PI3K), protein kinase B (PKB/Akt) and glucose transporter 4 (Glut4) and decreased the expression of glycogen synthase kinase 3β (GSK3β). We concluded that JTXK granule is an effective drug for T2DM through regulating the PI3K/Akt signalling pathway in skeletal muscle.
authors.affiliation: Preclinical Medicine School, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Na
authors.initials: N
authors.lastname: Yu
authors.affiliation: Department of Endocrinology, Beijing University of Chinese Medicine Third Affiliated Hospital, Beijing, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Fang
authors.affiliation: Diabetes Research Center, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Zhao
authors.affiliation: Preclinical Medicine School, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Qianqian
authors.initials: Q
authors.lastname: Mu
authors.affiliation: Preclinical Medicine School, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Jiacheng
authors.initials: J
authors.lastname: Zuo
authors.affiliation: Preclinical Medicine School, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Preclinical Medicine School, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Diabetes Research Center, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Fangfang
authors.initials: F
authors.lastname: Mo
authors.affiliation: Diabetes Research Center, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Dongwei
authors.initials: D
authors.lastname: Zhang
authors.affiliation: Diabetes Research Center, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Guangjian
authors.initials: G
authors.lastname: Jiang
authors.affiliation: Department of Endocrinology, South Area of Guang'anmen Hospital, China Academy of Chinese Medical Sciences, Beijing, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: Diabetes Research Center, Beijing University of Chinese Medicine, Beijing, China.
authors.forename: Sihua
authors.initials: S
authors.lastname: Gao
mesh_terms: D000818:Animals; D001786:Blood Glucose / Q000378:metabolism; D001835:Body Weight / Q000187:drug effects; D003924:Diabetes Mellitus, Type 2 / Q000097:blood / Q000188:drug therapy*; D004365:Drugs, Chinese Herbal / Q000494:pharmacology / Q000627:therapeutic use*; D005227:Fatty Acids / Q000097:blood; D005247:Feeding Behavior / Q000187:drug effects; D005951:Glucose Tolerance Test; D006442:Glycated Hemoglobin / Q000378:metabolism; D007004:Hypoglycemic Agents / Q000494:pharmacology / Q000627:therapeutic use*; D007328:Insulin / Q000097:blood; D007333:Insulin Resistance; D008297:Male; D008810:Mice, Inbred C57BL; D018482:Muscle, Skeletal / Q000378:metabolism; D019869:Phosphatidylinositol 3-Kinases / Q000378:metabolism*; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction / Q000187:drug effects
chemical_list: D001786:Blood Glucose; D004365:Drugs, Chinese Herbal; D005227:Fatty Acids; D006442:Glycated Hemoglobin; D007004:Hypoglycemic Agents; D007328:Insulin; D019869:Phosphatidylinositol 3-Kinases; D051057:Proto-Oncogene Proteins c-akt; D012333:RNA, Messenger; C000621855:jiang tang xiao ke