title: Cu@SnS/SnO2nanoparticles as novel sorbent for dispersive micro solid phase extraction of atorvastatin in human plasma and urine samples by high-performance liquid chromatography with UV detection: Application of central composite design (CCD).
abstract: Separation and detection of residual drug in biological fluids has directly related to human health in term of their beneficial or side effects. In biological fluid samples (complex matrix which containing drug at very low level) conduction of preliminary efficient technique as good choice for pre-concentration and clean-up of real sample before their instrumental analysis is highly recommended. In this result technique, based on nano-structure material which poses higher available surface area and larger member of reactive sites led to significant improvement in characteristic performance of analytical method. This paper focused on the synthesis and application of novel nano-sorbent for pre-concentration and extraction of atorvastatin (AT) from different biological fluids. Influence of various variables including sorbent dosage, eluent volume and sonication time on present method response was studied and optimized by central composite design under response surface methodology and accordingly an acceptable calibration curves over wide linear ranges (0.3-2000μgL-1) with high coefficient of determination higher than 0.999 strongly confirm high applicability of present method for quantification of analyte while limit of detection and quantification in plasma sample were 0.0608 and 0.2033μg L-1, respectively.
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Dastkhoon
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran. Electronic address: m_ghaedi@mail.yu.ac.ir.
authors.forename: Mehrorang
authors.initials: M
authors.lastname: Ghaedi
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Arash
authors.initials: A
authors.lastname: Asfaram
authors.affiliation: Chemistry Department, Yasouj University, Yasouj 75918-74831, Iran.
authors.forename: Maryam
authors.initials: M
authors.lastname: Arabi
authors.affiliation: Department of Chemistry, Kerman Branch, Islamic Azad University, Kerman, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Ostovan
authors.affiliation: Department of Polymer Engineering, Golestan University, Gorgan 49188-88369, Iran.
authors.forename: Alireza
authors.initials: A
authors.lastname: Goudarzi
mesh_terms: D000069059:Atorvastatin / Q000097:blood* / Q000302:isolation & purification / Q000652:urine*; D002851:Chromatography, High Pressure Liquid; D003300:Copper / Q000737:chemistry*; D006801:Humans; D057230:Limit of Detection; D053758:Nanoparticles / Q000737:chemistry*; D052617:Solid Phase Microextraction / Q000379:methods*; D012997:Solvents / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D013440:Sulfides / Q000737:chemistry*; D017971:Tin Compounds / Q000737:chemistry*
chemical_list: D012997:Solvents; D013440:Sulfides; D017971:Tin Compounds; C078041:tin sulfide; D003300:Copper; D000069059:Atorvastatin; C045358:stannic oxide
keywords: Atorvastatin; Central composite design; HPLC–UV; Human plasma samples; Plackett–Burman design; Ultrasound assisted extraction