title: Comparative genomic analysis of bacteriocin-producing Weissella cibaria 110.
abstract: Weissella cibaria 110 was isolated from plaa-som, a Thai fermented fish product, and known to produce the weissellicin 110 bacteriocin. We carried out comprehensive comparative genomic analysis of W. cibaria 110 with four other non-bacteriocin-producing W. cibaria strains and identified potential antibiotic-resistant genes. We further identified a type III restriction-modification system, a TA system, and a bacteriocin gene cluster that are unique in W. cibaria 110. Genes related to bacteriocin biosynthesis are organized in clusters and are encoded with minimum genetic machinery consisting of structural cognate immunity genes, including ABC transporter and immunity protein. Finally, we predicted W. cibaria 110 to produce a class IId bacteriocin, weissellicin 110, which is 31 amino acids in length and contains a 21-amino-acid N-terminal leader peptide. This is the first bacteriocin-producing sequencing genome in W. cibaria, and we describe the difference between the bacteriocin-producing and non bacteriocin-producing strains from genome point of view.
authors.affiliation: Institute of Biomedical Informatics, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei, 11221, Taiwan.|Bioinformatics Program, Taiwan International Graduate Program, Institute of Information Science, Academia Sinica, Taipei, 11529, Taiwan.
authors.forename: Shiao-Wen
authors.initials: SW
authors.lastname: Li
authors.affiliation: Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd, GuiShan Township, Taoyuan County, 333, Taiwan.
authors.forename: Yi-Sheng
authors.initials: YS
authors.lastname: Chen
authors.affiliation: Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd, GuiShan Township, Taoyuan County, 333, Taiwan.
authors.forename: Yun-Shien
authors.initials: YS
authors.lastname: Lee
authors.affiliation: Institute of Biomedical Informatics, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei, 11221, Taiwan.
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Yang
authors.affiliation: Faculty of Agro-Industry, Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat Campus, Thung Yai, 80240, Thailand.
authors.forename: Sirinat
authors.initials: S
authors.lastname: Srionnual
authors.affiliation: Department of Biotechnology, Ming Chuan University, No. 5, De-Ming Rd, GuiShan Township, Taoyuan County, 333, Taiwan.
authors.forename: Hui-Chung
authors.initials: HC
authors.lastname: Wu
authors.affiliation: Institute of Biomedical Informatics, National Yang-Ming University, No. 155, Sec 2, Linong Street, Taipei, 11221, Taiwan. cchang@ym.edu.tw.|Center for Systems and Synthetic Biology, National Yang-Ming University, Taipei, 11221, Taiwan. cchang@ym.edu.tw.
authors.forename: Chuan-Hsiung
authors.initials: CH
authors.lastname: Chang
mesh_terms: D000595:Amino Acid Sequence; D001430:Bacteriocins / Q000096:biosynthesis* / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification; D001483:Base Sequence; D005516:Food Microbiology; D016680:Genome, Bacterial; D023281:Genomics; D005810:Multigene Family; D010802:Phylogeny; D058836:Weissella / Q000145:classification / Q000235:genetics* / Q000276:immunology
chemical_list: D001430:Bacteriocins; C519800:weissellicin 110
keywords: Bacteriocin; Draft genome sequence; Probiotics; Weissella cibaria