title: Dynamic change in the NS3 protease domain in HIV/HCV-coinfected patients naïve to anti-HCV protease inhibitors.
abstract: The genetic analysis of THE natural protease inhibitors (PI) resistance of HCV genotype (GT)1 involves subtypes 1a and 1b. NS3 protease domain was sequentially analysed in 10 HIV/HCV GT1-coinfected individuals naïve to HCV treatment. Analysis at different time points showed that 2/3 GT1b patients were infected by a GT1a clade1 during follow-up. In one patient a switch from clade1 to clade2 and in one other patient a switch from clade2 to clade1 was revealed. Four out of ten patients had resistance-associated substitutions (RASs) at baseline. The dynamics of the dominant infecting subtype suggests the presence of mixed infection in some patients.
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.|Vita-Salute University, Milan, Italy.
authors.forename: Simona
authors.initials: S
authors.lastname: Bagaglio
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Messina
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.
authors.forename: Hamid
authors.initials: H
authors.lastname: Hasson
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Merli
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Andolina
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.|Vita-Salute University, Milan, Italy.
authors.forename: Adriano
authors.initials: A
authors.lastname: Lazzarin
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.
authors.forename: Caterina
authors.initials: C
authors.lastname: Uberti-Foppa
authors.affiliation: Department of Infectious Diseases, Ospedale San Raffaele, Milan, Italy.
authors.forename: Giulia
authors.initials: G
authors.lastname: Morsica
mesh_terms: D000998:Antiviral Agents / Q000494:pharmacology; D015967:Gene Expression Regulation, Viral / Q000187:drug effects; D005838:Genotype; D015658:HIV Infections / Q000150:complications*; D016174:Hepacivirus / Q000235:genetics* / Q000378:metabolism; D006526:Hepatitis C / Q000150:complications* / Q000188:drug therapy*; D006801:Humans; D010802:Phylogeny; D011480:Protease Inhibitors / Q000627:therapeutic use*; D019562:Viral Load; D017361:Viral Nonstructural Proteins / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
chemical_list: D000998:Antiviral Agents; D011480:Protease Inhibitors; D017361:Viral Nonstructural Proteins; C084422:NS3 protein, hepatitis C virus; D012697:Serine Endopeptidases; D043583:Nucleoside-Triphosphatase; D053487:DEAD-box RNA Helicases; D000085242:Viral Proteases
keywords: HIV/HCV coinfection; NS3 protease inhibitors; RAS