title: Antifungal Activity and Aflatoxin Degradation ofBifidobacterium BifidumandLactobacillus FermentumAgainst ToxigenicAspergillus Parasiticus.
abstract: Food and feedstuff contamination with aflatoxins (AFTs) is a serious health problem for humans and animals, especially in developing countries. The present study evaluated antifungal activities of two lactic acid bacteria (LAB)against growth and aflatoxin production of toxigenicAspergillus parasiticus. The mycelial growth inhibition rate ofA. parasiticusPTCC 5286 was investigated in the presence ofBifidobacterium bifidumPTCC 1644 andLactobacillus fermentumPTCC 1744 by the pour plate method. After seven days incubation in yeast extract sucrose broth at 30°C, the mycelial mass was weighed after drying. The inhibitory activity of LAB metabolites against aflatoxin production byA. parasiticuswas evaluated using HPLC method.B. bifidumandL. fermentumsignificantly reduced aflatoxin production and growth rate ofA. parasiticusin comparison with the controls (p≤0.05). LAB reduced total aflatoxins and B1, B2, G1and G2fractions by more than 99%. Moreover, LAB metabolites reduced the level of standard AFB1, B2, G1and G2from 88.8% to 99.8% (p≤0.05). Based on these findings,B. bifidumandL. fermentumare recommended as suitable biocontrol agents against the growth and aflatoxin production by aflatoxigenicAspergillusspecies.
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Roshanak Daie
authors.initials: RD
authors.lastname: Ghazvini
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ebrahim
authors.initials: E
authors.lastname: Kouhsari
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Ensieh
authors.initials: E
authors.lastname: Zibafar
authors.affiliation: Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran; Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Jamal
authors.initials: SJ
authors.lastname: Hashemi
authors.affiliation: Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Abolfazl
authors.initials: A
authors.lastname: Amini
authors.affiliation: Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Niknejad
keywords: Aflatoxin; Antifungal activity; Aspergillus parasiticus; Bifidobacterium bifidum; Lactobacillus fermentum