title: On-line MSPD-SPE-HPLC/FLD analysis of polycyclic aromatic hydrocarbons in bovine tissues.
abstract: A fast method was optimized and validated for simultaneous trace determination of four polycyclic aromatic hydrocarbons: benzo[a]anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene and benzo[a]pyrene in bovine tissues. The determination was performed by matrix solid-phase dispersion (MSPD) coupled on-line to solid phase extraction (SPE) and high performance liquid chromatography (HPLC) with fluorescence detection (FLD). The sample was dispersed on C18silica sorbent and then the on-line MSPD-SPE-HPLC/FLD method was applied. Several parameters were optimized: cleaning and elution sequences applied to the MSPD cartridge, the flow rate and dilution of extract used for SPE loading. The on-line method was validated over a concentration range of 0.1-0.6ngg-1obtaining good linearity (r⩾0.998) and precision (RSD)⩽10%. Recovery ranged from 96 to 99% and the limits of detection were 0.012ngg-1. This methodology was applied to liver samples from unhealthy animals. The results demonstrate that MSDP-SPE-HPLC/FLD method provides reliable, sensitive, accurate and fast data to the food control.
authors.affiliation: Laboratorio de Análisis de Trazas, Departamento de Química Analítica, Facultad de Química de la Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, 04510, Mexico; Departamento de Química, Facultad de Ciencias Naturales, Exactas y de la Educación, Universidad del Cauca, Sector Tulcán, 190003 Popayán, Colombia. Electronic address: tgutierrez@unicauca.edu.co.
authors.forename: Tania M
authors.initials: TM
authors.lastname: Gutiérrez-Valencia
authors.affiliation: Laboratorio de Análisis de Trazas, Departamento de Química Analítica, Facultad de Química de la Universidad Nacional Autónoma de México (UNAM), Ciudad Universitaria, 04510, Mexico.
authors.forename: Martha P
authors.initials: MP
authors.lastname: García de Llasera
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000032:analysis / Q000378:metabolism; D002417:Cattle; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D005449:Fluorenes / Q000032:analysis / Q000378:metabolism; D008099:Liver / Q000737:chemistry* / Q000378:metabolism*; D011084:Polycyclic Aromatic Hydrocarbons / Q000032:analysis* / Q000378:metabolism*; D052616:Solid Phase Extraction / Q000379:methods*; D014018:Tissue Distribution / Q000502:physiology
chemical_list: D005449:Fluorenes; D011084:Polycyclic Aromatic Hydrocarbons; D001564:Benzo(a)pyrene; C006703:benzo(b)fluoranthene; C022921:benzo(k)fluoranthene
keywords: Benzo[a]anthracene (PubChem CID: 5954); Benzo[a]pyrene (PubChem CID: 2336); Benzo[b]fluoranthene (PubChem CID: 9153); Benzo[k]fluoranthene (PubChem CID: 9158); Bovine tissues; HPLC analysis; Matrix solid-phase dispersion; On-line coupling analysis; Polycyclic aromatic hydrocarbons