title: Control of nuclear organization by F-actin binding proteins.
abstract: The regulation of nuclear shape and deformability is a key factor in controlling diverse events from embryonic development to cancer cell metastasis, but the mechanisms governing this process are still unclear. Our recent study demonstrated an unexpected role for the F-actin bundling protein fascin in controlling nuclear plasticity through a direct interaction with Nesprin-2. Nesprin-2 is a component of the LINC complex that is known to couple the F-actin cytoskeleton to the nuclear envelope. We demonstrated that fascin, which is predominantly associated with peripheral F-actin rich filopodia, binds directly to Nesprin-2 at the nuclear envelope in a range of cell types. Depleting fascin or specifically blocking the fascin-Nesprin-2 complex leads to defects in nuclear polarization, movement and cell invasion. These studies reveal a novel role for an F-actin bundling protein in control of nuclear plasticity and underline the importance of defining nuclear-associated roles for F-actin binding proteins in future.
authors.affiliation: a Randall Division of Cell and Molecular Biophysics , King's College London, New Hunts House , Guys Campus, London , UK.
authors.forename: Karin
authors.initials: K
authors.lastname: Pfisterer
authors.affiliation: a Randall Division of Cell and Molecular Biophysics , King's College London, New Hunts House , Guys Campus, London , UK.|b Department of Basic Sciences , Physiology Unit, San Pablo CEU University , Monteprincipe Campus, Madrid , Spain.
authors.forename: Asier
authors.initials: A
authors.lastname: Jayo
authors.affiliation: a Randall Division of Cell and Molecular Biophysics , King's College London, New Hunts House , Guys Campus, London , UK.
authors.forename: Maddy
authors.initials: M
authors.lastname: Parsons
mesh_terms: D000818:Animals; D002352:Carrier Proteins / Q000378:metabolism; D003599:Cytoskeleton / Q000378:metabolism; D006801:Humans; D008840:Microfilament Proteins / Q000378:metabolism*; D009685:Nuclear Envelope / Q000378:metabolism; D021381:Protein Transport
chemical_list: D002352:Carrier Proteins; D008840:Microfilament Proteins; C020324:fascin
keywords: Fascin; Nesprin-2; actin; cell migration; nuclear deformation; nuclear movement