title: Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion.
abstract: We describe a droplet microfluidics method to screen for multiple mutations of a same oncogene in a single experiment using passive droplet fusion. Genomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the KRAS oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly showing that the technique is quantitative.
authors.affiliation: CNRS, Univ. Bordeaux, CRPP, UPR 8641, 115 Avenue Schweitzer, F-33600, Pessac, France.
authors.forename: Deniz
authors.initials: D
authors.lastname: Pekin
authors.affiliation: INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, 45 rue des Saints-Pères, 75270, Paris Cedex 06, France. valerie.taly@parisdescartes.fr.
authors.forename: Valerie
authors.initials: V
authors.lastname: Taly
mesh_terms: D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis / Q000379:methods*; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D060885:Multiplex Polymerase Chain Reaction / Q000379:methods; D009154:Mutation; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods; D013223:Statistics as Topic / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor
keywords: Biomarkers; Cancer; Digital PCR; Droplet microfluidics; KRAS; Multiplexing; Passive droplet fusion