title: Description of a new anaerobic thermophilic bacterium, Thermoanaerobacterium butyriciformans sp. nov.
abstract: Strain USBA-019T, an anaerobic and thermophilic strain, was identified as a new member of the genus Thermoanaerobacterium. USBA-019Tcells are gram-positive, strictly anaerobic, thermophilic, chemoorganotrophic, moderately acidophilic, non-motile, endospore-forming, slightly curved, and rod-shaped. Cells measure 0.4×3.0-7.0μm. Optimal growth occurs at 50-55°C (35-65°C). Optimum pH is 5.0-5.5 (4.0-8.5). Thiosulfate, elemental sulfur and nitrate were utilized as electron acceptors. Fermentation of glucose, lactose, cellobiose, galactose, arabinose, xylose, starch and xylan primarily produced acetate and butyrate. Xylan, starch and cellobiose produced ethanol and starch, cellobiose, galactose, arabinose and mannose produced lactic acid. Phylogenetic analyses based on 16S rRNA gene sequence comparison and genomic relatedness indices show the close relation of USBA-019Tto Thermoanaerobacterium thermostercoris and Thermoanaerobacterium aotearoense (similarity value: 99%). Hybridization of USBA-019T, Th. thermostercoris DSM22141Tand Th. aotearoense DMS10170Tfound DNA-DNA relatedness of 33.2% and 18.2%, respectively. Based on phenotypic, chemotaxonomic and phylogenetic evidence, along with low identity at whole genome level, USBA-019Tis a novel species of the genus Thermoanaerobacterium which we propose to name Thermoanaerobacterium butyriciformans sp. nov. The type strain is USBA-019T(=CMPUJ U-019T=DSM 101588T).
authors.affiliation: Departamento de Biología, Pontificia Universidad Javeriana, POB 56710, Bogotá D.C., Colombia.
authors.forename: G
authors.initials: G
authors.lastname: López
authors.affiliation: Biological Sciences Department, Universidad de los Andes, Cra 1 No. 18A - 12, Bogotá D.C., Colombia; Department of Systems Biology, Harvard Medical School, Boston, MA 02115, USA.
authors.forename: S J
authors.initials: SJ
authors.lastname: Cañas-Duarte
authors.affiliation: Grupo de Investigación en Bioinformática y Biología de Sistemas, Instituto de Genética, Universidad Nacional de Colombia, Bogotá D.C., Colombia.
authors.forename: A M
authors.initials: AM
authors.lastname: Pinzón-Velasco
authors.affiliation: Centro de Bioinformática y Biología Computacional de Colombia-BIOS, Manizales, Colombia.
authors.forename: N E
authors.initials: NE
authors.lastname: Vega-Vela
authors.affiliation: Centro de Bioinformática y Biología Computacional de Colombia-BIOS, Manizales, Colombia.
authors.forename: M
authors.initials: M
authors.lastname: Rodríguez
authors.affiliation: Biological Sciences Department, Universidad de los Andes, Cra 1 No. 18A - 12, Bogotá D.C., Colombia.
authors.forename: S
authors.initials: S
authors.lastname: Restrepo
authors.affiliation: Departamento de Biología, Pontificia Universidad Javeriana, POB 56710, Bogotá D.C., Colombia. Electronic address: baena@javeriana.edu.co.
authors.forename: S
authors.initials: S
authors.lastname: Baena
mesh_terms: D000438:Alcohols / Q000378:metabolism; D000693:Anaerobiosis; D015373:Bacterial Typing Techniques; D050260:Carbohydrate Metabolism; D002264:Carboxylic Acids / Q000378:metabolism; D016000:Cluster Analysis; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D004275:DNA, Ribosomal / Q000737:chemistry / Q000235:genetics; D005285:Fermentation; D045482:Hot Springs / Q000382:microbiology*; D006863:Hydrogen-Ion Concentration; D009566:Nitrates / Q000378:metabolism; D009693:Nucleic Acid Hybridization; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D013171:Spores, Bacterial / Q000166:cytology; D013455:Sulfur / Q000378:metabolism; D013696:Temperature; D046950:Thermoanaerobacterium / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification*; D013885:Thiosulfates / Q000378:metabolism
chemical_list: D000438:Alcohols; D002264:Carboxylic Acids; D004269:DNA, Bacterial; D004275:DNA, Ribosomal; D009566:Nitrates; D012336:RNA, Ribosomal, 16S; D013885:Thiosulfates; D013455:Sulfur
keywords: Genomic relatedness indexes; Hot springs; New species; Taxonomic; Thermoanaerobacterium