title: Epithelial to mesenchymal transition and HPV infection in squamous cell oropharyngeal carcinomas: the papillophar study.
abstract: Human Papillomavirus (HPV) infection is recognised as aetiological factor of carcinogenesis in oropharyngeal squamous cell carcinomas (OPC). HPV-related OPC respond better to treatments and have a significantly favourable outcome. Epithelial to mesenchymal transition (EMT) implicated in tumour invasion, is a hallmark of a poor prognosis in carcinomas.
  
  
  
  We have studied the relationship of EMT markers (E-cadherin, β-catenin and vimentin) with HPV infection (DNA and E6/E7 mRNA detection), p16INK4aexpression and survival outcomes in a cohort of 296 patients with OPC.
  
  
  
  Among the 296 OPSSC, 26% were HPV positive, 20.3% had overt EMT (>25% of vimentin positive tumour cells). Lower E-cadherin expression was associated with a higher risk of distant metastasis in univariate (P=0.0110) and multivariate analyses (hazard ratios (HR)=6.86 (1.98; 23.84)). Vimentin expression tends towards worse metastasis-free survival (MFS; HR=2.53 (1.00; 6.41)) and was an independent prognostic factor of progression-free survival (HR=1.55 (1.03; 2.34)).
  
  
  
  There was a non significant association of EMT with HPV status. This may be explained by a mixed subpopulation of patients HPV positive with associated risk factors (HPV, tobacco and alcohol). Thus, the detection of EMT in OPC represents another reliable approach in the prognosis and the management of OPC whatever their HPV status.
authors.affiliation: Laboratoire d'Anatomie Pathologique, Hôpital Tenon, 4, rue de la Chine, Université Pierre et Marie Curie Paris 6,75050 Paris, France.
authors.forename: Marine
authors.initials: M
authors.lastname: Lefevre
authors.affiliation: AP-HP Hôpital Saint Antoine, URC-Est, 184 rue du Faubourg Saint Antoine, Université Pierre et Marie Curie Paris 6, 75012 Paris, France.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Rousseau
authors.affiliation: AP-HP Hôpital Saint Antoine, URC-Est, 184 rue du Faubourg Saint Antoine, Université Pierre et Marie Curie Paris 6, 75012 Paris, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Rayon
authors.affiliation: INSERM UMR-S 903, Université de Reims-Champagne-Ardenne and Laboratoire de Biopathologie CHU Maison Blanche, 45, rue Cognacq-Jay, 51100 Reims, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Dalstein
authors.affiliation: INSERM UMR-S 903, Université de Reims-Champagne-Ardenne and Laboratoire de Biopathologie CHU Maison Blanche, 45, rue Cognacq-Jay, 51100 Reims, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Clavel
authors.affiliation: Unité de Virologie, CHU de POITIERS, Faculté de Médecine de Poitiers Equipe émergente 2RCT, Université de Poitiers, Poitiers 86000, France.
authors.forename: Agnès
authors.initials: A
authors.lastname: Beby-Defaux
authors.affiliation: Université de Franche Comté, COMUE UBFC, 25000 Besancon, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Pretet
authors.affiliation: Laboratoire de Virologie, Hôpital Tenon, 4, rue de la Chine, 75050 Paris, France.|INSERM U1135 Université Pierre et Marie Curie, Paris 6, Paris 75000, France.
authors.forename: Patrick
authors.initials: P
authors.lastname: Soussan
authors.affiliation: INSERM UMR-S 903, Université de Reims-Champagne-Ardenne and Laboratoire de Biopathologie CHU Maison Blanche, 45, rue Cognacq-Jay, 51100 Reims, France.
authors.forename: Myriam
authors.initials: M
authors.lastname: Polette
authors.affiliation: AP-HP Hôpital Tenon, Service d'Oto-Rhino-Laryngologie, 4, rue de la Chine, Université Pierre et Marie Curie Paris 6, 75050 Paris, France.
authors.forename: Jean
authors.initials: J
authors.lastname: Lacau Saint Guily
authors.affiliation: INSERM UMR-S 903, Université de Reims-Champagne-Ardenne and Laboratoire de Biopathologie CHU Maison Blanche, 45, rue Cognacq-Jay, 51100 Reims, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Birembaut
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis*; D002294:Carcinoma, Squamous Cell / Q000150:complications / Q000175:diagnosis* / Q000628:therapy / Q000821:virology; D004279:DNA, Viral / Q000032:analysis; D018572:Disease-Free Survival; D058750:Epithelial-Mesenchymal Transition / Q000502:physiology*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009959:Oropharyngeal Neoplasms / Q000150:complications / Q000175:diagnosis* / Q000628:therapy / Q000821:virology; D027383:Papillomaviridae / Q000235:genetics; D030361:Papillomavirus Infections / Q000150:complications / Q000175:diagnosis* / Q000235:genetics; D011379:Prognosis
chemical_list: D014408:Biomarkers, Tumor; D004279:DNA, Viral