title: miR-2861 is involved in osteogenic commitment of human periodontal ligament stem cells grown onto 3D scaffold.
abstract: miR-2861 endorsing osteoblast differentiation through the overexpression of Runt-related transcription factor 2 (RUNX2) protein has been recently described. In this study we evaluated: the performance of living construct, composed by human Periodontal Ligament Stem Cells (hPDLSCs) and 3D scaffold (EXg), and the behaviour of miR-2861/RUNX2 expression pathway on the osteogenic commitment. Human PDLSCs were seeded with and without EXg scaffold and cultured under basal and osteogenic conditions. Morphological features, adhesiveness and differentiation abilities were analysed using scanning electron and confocal laser scanning microscopy. Time-course of RUNX2, ALP, OPN and miR-2861 were evaluated through RT-PCR analysis. Our results highlighted that the osteogenic differentiation was mostly obvious in the hPDLSCs, grown onto 3D scaffold in presence of osteoinductive medium. Moreover, the overexpression of miR-2861 and RUNX2 in hPDLSCs cultured in presence of EXg under osteogenic and standard conditions was demonstrated. In synthesis, the increased expression of miR-2861/RUNX2 provides new insights regarding miRNA signaling network in the presence of scaffold providing an additional method to evaluate the performance of biomaterial in bone regeneration.
authors.affiliation: Laboratory of Stem Cells and Regenerative Medicine, University “G. d’Annunzio”, Chieti-Pescara, Chieti, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Diomede
authors.affiliation: Laboratory of Stem Cells and Regenerative Medicine, University “G. d’Annunzio”, Chieti-Pescara, Chieti, Italy.
authors.forename: I
authors.initials: I
authors.lastname: Merciaro
authors.affiliation: Department of Pharmacy, University “G. d’Annunzio”; Chieti-Pescara, Chieti, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Martinotti
authors.affiliation: UMR 7365 CNRS-UL IMoPA et FR UL-CNRS-INSERM, Faculté de Médecine, Lorraine University Vandoeuvre-les-Nancy, France.|Department of Medical, Oral and Biotechnological Sciences; University “G. d’Annunzio”, Chieti-Pescara, Chieti, Italy.
authors.forename: M F X B
authors.initials: MF
authors.lastname: Cavalcanti
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences; University “G. d’Annunzio”, Chieti-Pescara, Chieti, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Caputi
authors.affiliation: IRCCS Centro Neurolesi “Bonino-Pulejo”, Messina, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Mazzon
authors.affiliation: Laboratory of Stem Cells and Regenerative Medicine, University “G. d’Annunzio”, Chieti-Pescara, Chieti, Italy.
authors.forename: O
authors.initials: O
authors.lastname: Trubiani
mesh_terms: D000818:Animals; D001861:Bone Regeneration / Q000235:genetics; D002454:Cell Differentiation / Q000235:genetics; D002478:Cells, Cultured; D050659:Core Binding Factor Alpha 1 Subunit / Q000096:biosynthesis; D005434:Flow Cytometry; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D010012:Osteogenesis / Q000235:genetics*; D010513:Periodontal Ligament / Q000166:cytology*; D060888:Real-Time Polymerase Chain Reaction; D013234:Stem Cells / Q000166:cytology*; D013552:Swine; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D050659:Core Binding Factor Alpha 1 Subunit; C583983:MIRN2861 microRNA, human; D035683:MicroRNAs; C493709:RUNX2 protein, human