title: Superoxide dismutase 3 attenuates experimental Th2-driven allergic conjunctivitis.
abstract: Allergic conjunctivitis is an inflammatory eye disease mediated by Th2 type immune response. The role of extracellular superoxide dismutase 3 (SOD3) in immune response and allergic conjunctival inflammation was examined in a murine model for experimental allergic conjunctivitis (EAC). Allergic conjunctivitis was induced in mice by allergen challenge with ovalbumin in alum via the conjunctival sac. SOD3 was topically applied and allergy indicators were compared. Clinical signs associated with conjunctivitis, such as OVA-specific IgE production, IgG1/G2a ratio and eosinophil infiltration, were drastically reduced in mice treated with SOD3. They also had less dendritic cells and CD4+T cells in conjunctiva than controls. Attenuated allergic inflammation was accredited to reduced Th2 type cytokine responses and increased Treg cytokine in draining lymph node. The characteristics of EAC were attributed to the absence of SOD3. Our findings suggest that SOD3 might be considered as a potential target for Th2-driven allergic conjunctival inflammation.
authors.affiliation: Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea; Catholic Institute for Visual Science, Catholic University of Korea, College of Medicine, Seoul, Republic of Korea.
authors.forename: Hyun Jung
authors.initials: HJ
authors.lastname: Lee
authors.affiliation: Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea; Catholic Institute for Visual Science, Catholic University of Korea, College of Medicine, Seoul, Republic of Korea.
authors.forename: Bo-Mi
authors.initials: BM
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea; Catholic Institute for Visual Science, Catholic University of Korea, College of Medicine, Seoul, Republic of Korea.
authors.forename: Soojung
authors.initials: S
authors.lastname: Shin
authors.affiliation: Laboratory of Dermato-Immunology, Catholic Research Institute of Medical Science, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea. Electronic address: tykimder@catholic.ac.kr.
authors.forename: Tae-Yoon
authors.initials: TY
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, College of Medicine, Catholic University of Korea, Seoul, Republic of Korea; Catholic Institute for Visual Science, Catholic University of Korea, College of Medicine, Seoul, Republic of Korea. Electronic address: chungsh@catholic.ac.kr.
authors.forename: So-Hyang
authors.initials: SH
authors.lastname: Chung
mesh_terms: D000485:Allergens / Q000276:immunology; D000818:Animals; D003228:Conjunctiva / Q000276:immunology; D003233:Conjunctivitis, Allergic / Q000276:immunology*; D016207:Cytokines / Q000276:immunology; D004195:Disease Models, Animal; D004804:Eosinophils / Q000276:immunology; D005260:Female; D007073:Immunoglobulin E / Q000276:immunology; D007074:Immunoglobulin G / Q000276:immunology; D007249:Inflammation / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D010047:Ovalbumin / Q000276:immunology; D013482:Superoxide Dismutase / Q000276:immunology*; D018418:Th2 Cells / Q000276:immunology*
chemical_list: D000485:Allergens; D016207:Cytokines; D007074:Immunoglobulin G; D007073:Immunoglobulin E; D010047:Ovalbumin; C499090:Sod3 protein, mouse; D013482:Superoxide Dismutase
keywords: Allergic conjunctivitis; Eosinophil; IgE; SOD3; Th2 type immune response