title: Comparison of cellular and transcriptomic effects between electronic cigarette vapor and cigarette smoke in human bronchial epithelial cells.
abstract: The use of electronic cigarette (e-cig) can be considered as an alternative to smoking. However, due to a lack of thorough toxicological studies, absolute safety of these products cannot be guaranteed. The aim of this in vitro work was to investigate the potential toxicity of e-vapors generated by a smoking machine in human bronchial epithelial BEAS-2B cells cultured at air-liquid interface, in comparison to cigarette smoke (CS). Although CS decreased strongly cell viability from 48min exposure, e-vapors induced no cytotoxicity up to 288min exposure. Moreover, oxidative stress was evidenced only after exposure to CS, with a decrease secretion of GRO-ɑ from 8min and of IL-8 and MCP-1 after 48min exposure. Only a low increase of IL-6 secretion was measured in cells exposed to e-vapors. Finally, transcriptomic data of exposed cells indicated that a large number of genes were deregulated in response to CS, especially genes involved in important biological functions as oxidative stress and cell death, while e-vapors elicited very discrete modulation. These results strongly suggest a lower toxicity of e-vapors compared to CS in the BEAS-2B cell line and constitute a baseline for further experimental studies with a larger spectrum of e-liquids and e-cig models.
authors.affiliation: Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA 4483, IMPECS - IMPact of Environmental ChemicalS on human health, F-59000 Lille, France. Electronic address: sebastien.antherieu@univ-lille2.fr.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Anthérieu
authors.affiliation: Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA 4483, IMPECS - IMPact of Environmental ChemicalS on human health, F-59000 Lille, France.
authors.forename: Anne
authors.initials: A
authors.lastname: Garat
authors.affiliation: Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA 4483, IMPECS - IMPact of Environmental ChemicalS on human health, F-59000 Lille, France; Mines Douai, SAGE, F-59508 Douai, France.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Beauval
authors.affiliation: Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA 4483, IMPECS - IMPact of Environmental ChemicalS on human health, F-59000 Lille, France.
authors.forename: Mélissa
authors.initials: M
authors.lastname: Soyez
authors.affiliation: Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA 4483, IMPECS - IMPact of Environmental ChemicalS on human health, F-59000 Lille, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Allorge
authors.affiliation: Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA 4483, IMPECS - IMPact of Environmental ChemicalS on human health, F-59000 Lille, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Garçon
authors.affiliation: Univ. Lille, CHU Lille, Institut Pasteur de Lille, EA 4483, IMPECS - IMPact of Environmental ChemicalS on human health, F-59000 Lille, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Lo-Guidice
mesh_terms: D000255:Adenosine Triphosphate / Q000032:analysis / Q000378:metabolism; D002470:Cell Survival / Q000187:drug effects; D066300:Electronic Nicotine Delivery Systems; D005740:Gases; D005786:Gene Expression Regulation / Q000187:drug effects; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000139:chemically induced / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D012906:Smoke / Q000009:adverse effects*; D014026:Nicotiana / Q000009:adverse effects; D014028:Tobacco Smoke Pollution / Q000009:adverse effects*; D059467:Transcriptome / Q000187:drug effects*
chemical_list: D005740:Gases; D012906:Smoke; D014028:Tobacco Smoke Pollution; D000255:Adenosine Triphosphate; D005978:Glutathione
keywords: BEAS-2B cell line; E-cigarette; Toxicity; Transcriptomic; Vapors