title: DNA Methylation Profiles of Selected Pro-Inflammatory Cytokines in Alzheimer Disease.
abstract: By means of functional genomics analysis, we recently described the mRNA expression profiles of various genes involved in the neuroinflammatory response in the brains of subjects with late-onset Alzheimer Disease (LOAD). Some of these genes, namely interleukin (IL)-1β and IL-6, showed distinct expression profiles with peak expression during the first stages of the disease and control-like levels at later stages. IL-1β and IL-6 genes are modulated by DNA methylation in different chronic and degenerative diseases; it is also well known that LOAD may have an epigenetic basis. Indeed, we and others have previously reported gene-specific DNA methylation alterations in LOAD and in related animal models. Based on these data, we studied the DNA methylation profiles, at single cytosine resolution, of IL-1β and IL-6 5'-flanking region by bisulphite modification in the cortex of healthy controls and LOAD patients at 2 different disease stages: Braak I-II/A and Braak V-VI/C. Our analysis provides evidence that neuroinflammation in LOAD is associated with (and possibly mediated by) epigenetic modifications.
authors.affiliation: Department of Surgery "P. Valdoni", Sapienza University of Rome, Italy.
authors.forename: Vincenzina
authors.initials: V
authors.lastname: Nicolia
authors.affiliation: Department of Surgery "P. Valdoni", Sapienza University of Rome, Italy.
authors.forename: Rosaria A
authors.initials: RA
authors.lastname: Cavallaro
authors.affiliation: Neuropathology, Service of Pathology, Bellvitge University Hospital; and University of Barcelona, Hospitalet de LLobregat, Spain.
authors.forename: Irene
authors.initials: I
authors.lastname: López-González
authors.affiliation: European Center for Brain Research (CERC)/IRCCS Santa Lucia Foundation, Rome, Italy.|Department of Medicine, Campus Bio-Medico University of Rome, Rome, Italy.
authors.forename: Mauro
authors.initials: M
authors.lastname: Maccarrone
authors.affiliation: Department of Surgery "P. Valdoni", Sapienza University of Rome, Italy.
authors.forename: Sigfrido
authors.initials: S
authors.lastname: Scarpa
authors.affiliation: Department of Surgery "P. Valdoni", Sapienza University of Rome, Italy.|Neuropathology, Service of Pathology, Bellvitge University Hospital; and University of Barcelona, Hospitalet de LLobregat, Spain.
authors.forename: Isidre
authors.initials: I
authors.lastname: Ferrer
authors.affiliation: European Center for Brain Research (CERC)/IRCCS Santa Lucia Foundation, Rome, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Fuso
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D016207:Cytokines / Q000235:genetics / Q000378:metabolism*; D019175:DNA Methylation / Q000502:physiology*; D005260:Female; D005625:Frontal Lobe / Q000378:metabolism / Q000473:pathology; D020869:Gene Expression Profiling / Q000379:methods*; D006801:Humans; D018836:Inflammation Mediators / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D011401:Promoter Regions, Genetic / Q000502:physiology
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators
keywords: Alzheimer disease; Cytokines; DNA methylation; Epigenetics; Neuroinflammation; Non-CpG methylation