title: A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy.
abstract: Here we present mGarnet2, a monomeric, far-red fluorescent marker protein derived from mRuby, with absorption and emission bands peaking at 598 and 671 nm, respectively. The protein shows excellent performance as a live-cell fusion marker for STED nanoscopy with 640 nm excitation and 780 nm depletion wavelengths.
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Gabriela
authors.initials: G
authors.lastname: Matela
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany and Institute of Nanotechnology and Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany.
authors.forename: Peng
authors.initials: P
authors.lastname: Gao
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Gernot
authors.initials: G
authors.lastname: Guigas
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Antonia F
authors.initials: AF
authors.lastname: Eckert
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
authors.forename: Karin
authors.initials: K
authors.lastname: Nienhaus
authors.affiliation: Institute of Applied Physics, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany and Institute of Nanotechnology and Institute of Toxicology and Genetics, Karlsruhe Institute of Technology (KIT), Eggenstein-Leopoldshafen, Germany and Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA. uli@uiuc.edu.
authors.forename: G U
authors.identifier: 0000-0002-5027-3192
authors.initials: GU
authors.lastname: Nienhaus
mesh_terms: D008164:Luminescent Proteins / Q000737:chemistry*; D008856:Microscopy, Fluorescence; D036103:Nanotechnology; D000097573:Red Fluorescent Protein
chemical_list: D008164:Luminescent Proteins; D000097573:Red Fluorescent Protein