title: Nonomuraea ceibae sp. nov., an actinobacterium isolated from Ceiba speciosa rhizosphere.
abstract: Strain XMU 110T, isolated from the rhizosphere soil of a flowering tree, Ceiba speciosa, was characterized by polyphasic taxonomy. Phylogenetic analysis based on 16S rRNA gene comparisons revealed that strain XMU 110T showed the highest similarity of 97.9 % to Nonomuraea jabiensis DSM 45507T, and indicated the closest relatives were Nonomuraearoseoviolaceasubsp.roseoviolacea ATCC 27297T (97.8 % 16S rRNA gene sequence similarity) and Nonomuraea salmonea DSM 43678T (97.4 %) after a neighbour-joining analysis. The phenotypic characteristics, as well as the DNA-DNA relatedness values between strain XMU 110T and N. roseoviolaceasubsp. roseoviolacea ATCC 27297T (48.07±1.99 %) and N. salmonea DSM 43678T (40.55±8.30 %), distinguished the novel strain from its closest phylogenetic neighbours. The morphological, physiological and chemotaxonomic characteristics such as phospholipid type, diagnostic diamino acid of the peptidoglycan, whole-cell sugars, major menaquinones and major fatty acids further supported the assignment of strain XMU 110T to the genus Nonomuraea. The G+C content of the genomic DNA was 66.2 mol%. Based on the taxonomic data, strain XMU 110Trepresents a novel species of the genus Nonomuraea, for which the name Nonomuraea ceibae sp. nov. is proposed. The type strain is XMU 110T (=MCCC 1K03213T= KCTC 39826T).
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China.
authors.forename: Jindi
authors.initials: J
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China.
authors.forename: Yaojian
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 361102, PR China.
authors.forename: Xianming
authors.initials: X
authors.lastname: Deng
mesh_terms: D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D035164:Ceiba / Q000382:microbiology*; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D058441:Rhizosphere; D017422:Sequence Analysis, DNA; D012988:Soil Microbiology
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S