title: Surface charge-bias impact of amine-contained pseudozwitterionic biointerfaces on the human blood compatibility.
abstract: This work discusses the impact of the charge bias and the hydrophilicity on the human blood compatibility of pseudozwitterionic biomaterial gels. Four series of hydrogels were prepared, all containing negatively-charged 3-sulfopropyl methacrylate (SA), and either acrylamide, N-isopropylacrylamide, 2-dimethylaminoethyl methacrylate (DMAEMA) or [2-(methacryloyloxy)ethyl]trimethylammonium (TMA), to form SnAm, SnNm, SnDmor SnTmhydrogels, respectively. An XPS analysis proved that the polymerization was well controlled from the initial monomer ratios. All gels present high surface hydrophilicity, but varying bulk hydration, depending on the nature/content of the comonomer, and on the immersion medium. The most negative interfaces (pure SA, S7A3, S5A5) showed significant fibrinogen adsorption, ascribed to the interactions of the αC domains of the protein with the gels, then correlated to considerable platelet adhesion; but low leukocyte/erythrocyte attachments were measured. Positive gels (excess of DMAEMA or TMA) are not hemocompatible. They mediate protein adsorption and the adhesion of human blood cells, through electrostatic attractive interactions. The neutral interfaces (zeta potential between -10mV and +10mV) are blood-inert only if they present a high surface and bulk hydrophilicity. Overall, this study presents a map of the hemocompatible behavior of hydrogels as a function of their surface charge-bias, essential to the design of blood-contacting devices.
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taoyuan 320, Taiwan. Electronic address: avenault@cycu.edu.tw.
authors.forename: Antoine
authors.initials: A
authors.lastname: Venault
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taoyuan 320, Taiwan.
authors.forename: Ko-Jen
authors.initials: KJ
authors.lastname: Hsu
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taoyuan 320, Taiwan.
authors.forename: Lu-Chen
authors.initials: LC
authors.lastname: Yeh
authors.affiliation: Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Chinnathambi
authors.affiliation: Laboratory Medicine, Mackay Memorial Hospital, Taipei 104, Taiwan.
authors.forename: Hsin-Tsung
authors.initials: HT
authors.lastname: Ho
authors.affiliation: R&D Center for Membrane Technology and Department of Chemical Engineering, Chung Yuan Christian University, Chung-Li, Taoyuan 320, Taiwan. Electronic address: ychang@cycu.edu.tw.
authors.forename: Yung
authors.initials: Y
authors.lastname: Chang
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000327:Adsorption; D000588:Amines / Q000737:chemistry*; D001672:Biocompatible Materials / Q000737:chemistry*; D004912:Erythrocytes / Q000166:cytology / Q000187:drug effects*; D005340:Fibrinogen / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry; D007962:Leukocytes / Q000166:cytology / Q000187:drug effects*; D008689:Methacrylates / Q000737:chemistry*; D010973:Platelet Adhesiveness; D000072417:Protein Domains; D055672:Static Electricity; D013499:Surface Properties; D014867:Water / Q000737:chemistry
chemical_list: C120889:2-(trimethylammonio)ethyl methacrylate; C529206:3-sulfopropyl methacrylate; D000178:Acrylamides; D000588:Amines; D001672:Biocompatible Materials; D020100:Hydrogels; D008689:Methacrylates; D014867:Water; D005340:Fibrinogen; C067295:N-isopropylacrylamide; C049840:2-(dimethylamino)ethyl methacrylate
keywords: Blood compatibility control; Charge bias; Hydrogel interfaces; Poly(SA-co-amide/amine); Pseudozwitterionic biomaterials