title: Multilocus Phylogeny and Antifungal Susceptibility of Aspergillus Section Circumdati from Clinical Samples and Description of A. pseudosclerotiorum sp. nov.
abstract: A multilocus phylogenetic study was carried out to assess species identity of a set of 34 clinical isolates fromAspergillussectionCircumdatifrom the United States and to determine theirin vitroantifungal susceptibility against eight antifungal drugs. The genetic markers used were the internal transcribed spacer (ITS) region, and fragments of the beta-tubulin (BenA), calmodulin (CaM), and RNA polymerase II second largest subunit (RPB2) genes. The drugs tested were amphotericin B, itraconazole, posaconazole, voriconazole, anidulafungin, caspofungin, micafungin, and terbinafine. The most common species sampled wasA. westerdijkiae(29.4%), followed by a novel species, which was described here asA. pseudosclerotiorum(23.5%). Other species identified wereA. sclerotiorum(17.6%),A. ochraceus(8.8%),A. subramanianii(8.8%), andA. insulicolaandA. ochraceopetaliformis, with two isolates (5.9%) of each. The drugs that showed the most potent activity were caspofungin, micafungin, and terbinafine, while amphotericin B showed the least activity.
authors.affiliation: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.|Laboratório de Microbiologia, Faculdade de Medicina de São José do Rio Preto, São José do Rio Preto, Brazil.
authors.forename: J P Z
authors.initials: JP
authors.lastname: Siqueira
authors.affiliation: Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas, USA.
authors.forename: D A
authors.initials: DA
authors.lastname: Sutton
authors.affiliation: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain josepa.gene@urv.cat.
authors.forename: J
authors.identifier: 0000-0001-6195-9299
authors.initials: J
authors.lastname: Gené
authors.affiliation: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: D
authors.initials: D
authors.lastname: García
authors.affiliation: Fungus Testing Laboratory, University of Texas Health Science Center, San Antonio, Texas, USA.
authors.forename: N
authors.initials: N
authors.lastname: Wiederhold
authors.affiliation: Mycotoxin Prevention and Applied Microbiology Research Unit, National Center for Agricultural Utilization Research, Peoria, Illinois, USA.
authors.forename: S W
authors.initials: SW
authors.lastname: Peterson
authors.affiliation: Unitat de Micologia, Facultat de Medicina i Ciències de la Salut, IISPV, Universitat Rovira i Virgili, Reus, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Guarro
mesh_terms: D000935:Antifungal Agents / Q000494:pharmacology*; D001228:Aspergillosis / Q000382:microbiology*; D001230:Aspergillus / Q000145:classification* / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002147:Calmodulin / Q000235:genetics; D016000:Cluster Analysis; D004271:DNA, Fungal / Q000737:chemistry / Q000235:genetics; D021903:DNA, Ribosomal Spacer / Q000737:chemistry / Q000235:genetics; D006801:Humans; D008826:Microbial Sensitivity Tests; D010802:Phylogeny; D012319:RNA Polymerase II / Q000235:genetics; D017422:Sequence Analysis, DNA; D014404:Tubulin / Q000235:genetics; D014481:United States
chemical_list: D000935:Antifungal Agents; D002147:Calmodulin; D004271:DNA, Fungal; D021903:DNA, Ribosomal Spacer; D014404:Tubulin; D012319:RNA Polymerase II
keywords: Aspergillus; Circumdati section; clinical isolates; molecular identification; phenotypic identification