title: miR-375 and miR-205 Regulate the Invasion and Migration of Laryngeal Squamous Cell Carcinoma Synergistically via AKT-Mediated EMT.
abstract: Previous studies have found that miR-375 and miR-205 were significantly dysregulated in laryngeal squamous cell carcinoma, which contributed to the invasion and migration of LSCC. However, the mechanisms of miR-375 and miR-205 regulating the invasion and migration of LSCC remain unknown. qRT-PCR was performed in 40 pairs of tissue samples to investigate the expression of miR-375 and miR-205 in LSCC and paracarcinoma tissues. To investigate whether or not miR-375 and miR-205 regulated the invasion and migration of LSCC synergistically via AKT-mediated epithelial-mesenchymal transition, miR-375 mimic and miR-205 inhibitor were transfected into SNU899 cells and miR-375 inhibitor and miR-205 mimic were transfected into SNU899 cells, respectively, with or without AKT inhibitor. Then the expressions of miR-375 and miR-205 were validated by qRT-PCR, cell migration and invasion were determined by wound healing assay and transwell invasive assay, and western blot analysis was performed to detect the expression of related proteins. Our results showed that miR-375 and miR-205 regulated the invasion and migration of LSCC via AKT-mediated EMT synergistically. In conclusion, our findings provided not only new information about the molecular mechanism of miRNAs regulating invasion and migration of LSCC, but also a theoretical principle for potential targeting therapy of laryngeal squamous carcinoma.
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Otorhinolaryngology Institute, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Wang
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Otorhinolaryngology Institute, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Kexing
authors.initials: K
authors.lastname: Lv
authors.affiliation: Department of Otorhinolaryngology, The First People's Hospital of Foshan, Foshan, China.
authors.forename: Weixiong
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Otorhinolaryngology Institute, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Otorhinolaryngology Institute, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Otorhinolaryngology Institute, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Jianhui
authors.initials: J
authors.lastname: Wu
authors.affiliation: Department of Otorhinolaryngology, The First People's Hospital of Foshan, Foshan, China.
authors.forename: Zenghong
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Otorhinolaryngology, The First People's Hospital of Foshan, Foshan, China.
authors.forename: Hao
authors.initials: H
authors.lastname: Qin
authors.affiliation: Department of Surgery, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong.
authors.forename: Thian-Sze
authors.initials: TS
authors.lastname: Wong
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Otorhinolaryngology Institute, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Weiqiang
authors.initials: W
authors.lastname: Yang
authors.affiliation: Otorhinolaryngology Hospital, The First Affiliated Hospital, Sun Yat-Sen University, 58 Zhongshan Road II, Guangzhou, Guangdong 510080, China.
authors.forename: Qing-Ling
authors.initials: QL
authors.lastname: Fu
authors.affiliation: Department of Otorhinolaryngology, The First Affiliated Hospital of Sun Yat-Sen University, Otorhinolaryngology Institute, Sun Yat-Sen University, Guangzhou, China.
authors.forename: Wenbin
authors.identifier: 0000-0002-9720-1997
authors.initials: W
authors.lastname: Lei
mesh_terms: D000368:Aged; D017209:Apoptosis / Q000235:genetics; D002294:Carcinoma, Squamous Cell / Q000235:genetics* / Q000473:pathology; D002465:Cell Movement / Q000235:genetics; D049109:Cell Proliferation / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007822:Laryngeal Neoplasms / Q000235:genetics* / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000096:biosynthesis / Q000235:genetics*; D008875:Middle Aged; D009361:Neoplasm Invasiveness / Q000235:genetics; D051058:Oncogene Protein v-akt / Q000235:genetics*
chemical_list: D035683:MicroRNAs; D051058:Oncogene Protein v-akt; C534425:MIR205, human; C540933:MIRN375 microRNA, human