title: The biological properties of different Epstein-Barr virus strains explain their association with various types of cancers.
abstract: The Epstein-Barr virus (EBV) is etiologically associated with the development of multiple types of tumors, but it is unclear whether this diversity is due to infection with different EBV strains. We report a comparative characterization of SNU719, GP202, and YCCEL1, three EBV strains that were isolated from gastric carcinomas, M81, a virus isolated in a nasopharyngeal carcinoma and several well-characterized laboratory type A strains. We found that B95-8, Akata and GP202 induced cell growth more efficiently than YCCEL1, SNU719 and M81 and this correlated positively with the expression levels of the viral BHRF1 miRNAs. In infected B cells, all strains except Akata and B95-8 induced lytic replication, a risk factor for carcinoma development, although less efficiently than M81. The panel of viruses induced tumors in immunocompromised mice with variable speed and efficacy that did not strictly mirror their in vitro characteristics, suggesting that additional parameters play an important role. We found that YCCEL1 and M81 infected primary epithelial cells, gastric carcinoma cells and gastric spheroids more efficiently than Akata or B95-8. Reciprocally, Akata and B95-8 had a stronger tropism for B cells than YCCEL1 or M81. These data suggest that different EBV strains will induce the development of lymphoid tumors with variable efficacy in immunocompromised patients and that there is a parallel between the cell tropism of the viral strains and the lineage of the tumors they induce. Thus, EBV strains can be endowed with properties that will influence their transforming abilities and the type of tumor they induce.
authors.affiliation: German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany.|Inserm unit U1074, DKFZ, 69120 Heidelberg, Germany.
authors.forename: Ming-Han
authors.initials: MH
authors.lastname: Tsai
authors.affiliation: German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany.|Inserm unit U1074, DKFZ, 69120 Heidelberg, Germany.
authors.forename: Xiaochen
authors.initials: X
authors.lastname: Lin
authors.affiliation: German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany.|Inserm unit U1074, DKFZ, 69120 Heidelberg, Germany.
authors.forename: Anatoliy
authors.initials: A
authors.lastname: Shumilov
authors.affiliation: German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany.|Inserm unit U1074, DKFZ, 69120 Heidelberg, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Bernhardt
authors.affiliation: German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany.|Inserm unit U1074, DKFZ, 69120 Heidelberg, Germany.|Institute for Diabetes and Obesitas, Monoclonal Antibody Core Facility, German Research Center for Environmental Health, Helmholtz Zentrum München, 81377 Munich, Germany.
authors.forename: Regina
authors.initials: R
authors.lastname: Feederle
authors.affiliation: German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany.|Inserm unit U1074, DKFZ, 69120 Heidelberg, Germany.
authors.forename: Remy
authors.initials: R
authors.lastname: Poirey
authors.affiliation: German Cancer Research Centre (DKFZ), Unit C060, 69120 Heidelberg, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Kopp-Schneider
authors.affiliation: Differentiation and Cancer Group, IPATIMUP, Rua Dr Roberto Frias s/n, 4200 - 465 Porto, Portugal.
authors.forename: Bruno
authors.initials: B
authors.lastname: Pereira
authors.affiliation: Differentiation and Cancer Group, IPATIMUP, Rua Dr Roberto Frias s/n, 4200 - 465 Porto, Portugal.
authors.forename: Raquel
authors.initials: R
authors.lastname: Almeida
authors.affiliation: German Cancer Research Centre (DKFZ), Unit F100, 69120 Heidelberg, Germany.|Inserm unit U1074, DKFZ, 69120 Heidelberg, Germany.
authors.forename: Henri-Jacques
authors.initials: HJ
authors.lastname: Delecluse
mesh_terms: D000818:Animals; D001402:B-Lymphocytes / Q000378:metabolism / Q000473:pathology / Q000821:virology; D018938:Caco-2 Cells; D002277:Carcinoma / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D049109:Cell Proliferation; D002472:Cell Transformation, Viral; D018920:Coculture Techniques; D004847:Epithelial Cells / Q000378:metabolism / Q000473:pathology / Q000821:virology; D020031:Epstein-Barr Virus Infections / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D057809:HEK293 Cells; D004854:Herpesvirus 4, Human / Q000145:classification / Q000235:genetics / Q000302:isolation & purification / Q000472:pathogenicity*; D054884:Host-Pathogen Interactions; D006801:Humans; D016513:Mice, SCID; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D012367:RNA, Viral / Q000235:genetics / Q000378:metabolism; D013274:Stomach Neoplasms / Q000235:genetics / Q000378:metabolism / Q000473:pathology / Q000821:virology*; D013997:Time Factors; D014764:Viral Proteins / Q000235:genetics / Q000378:metabolism; D056189:Viral Tropism; D053586:Virus Internalization; D014779:Virus Replication
chemical_list: C083278:BHRF1 protein, Human herpesvirus 4; D035683:MicroRNAs; D012367:RNA, Viral; D014764:Viral Proteins
keywords: Epstein-Barr virus strains; carcinoma and lymphoma; host-virus interactions; human tumor viruses; viral infection and transformation