title: Light Intensity is Important for Hydrogen Production in NaHSO3-Treated Chlamydomonas reinhardtii.
abstract: Chlamydomonas reinhardtii is a unicellular green alga that can use light energy to produce H2 from H2O in the background of NaHSO3 treatment. However, the role of light intensity in such H2 production remains elusive. Here, light intensity significantly affected the yield of H2 production in NaHSO3-treated C. reinhardtii, which was consistent with its effects on the content of O2 and the expression and activity of hydrogenase. Further, NaHSO3 was found to be able to remove O2 via a reaction of bisulfite with superoxide anion produced at the acceptor side of PSI, and light intensity affected the reaction rate significantly. Accordingly, high light and strong light but not low light can create an anaerobic environment, which is important to activate hydrogenase and produce H2. Based on the above results, we conclude that light intensity plays an important role in removing O2 and consequently activating hydrogenase and producing H2 in NaHSO3-treated C. reinhardtii.
authors.affiliation: Chongqing Engineering Research Center for Rapeseed, College of Agronomy and Biotechnology, Southwest University, Chongqing, China.
authors.forename: Lanzhen
authors.initials: L
authors.lastname: Wei
authors.affiliation: College of Life Sciences, South China Agricultural University, Guangzhou, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yi
authors.affiliation: Institute of Applied Ecology, Chinese Academy of Sciences, Wenhua Road, Shenhe District, Shenyang, Liaoning, China.
authors.forename: Lianjun
authors.initials: L
authors.lastname: Wang
authors.affiliation: Lab of Brain and Gut Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon TongHong Kong SAR, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Shuangqing Road, Haidian District, Beijing, China
authors.forename: Fudan
authors.initials: F
authors.lastname: Gao
authors.affiliation: College of Life Sciences, Capital Normal University, Beijing, China.
authors.forename: Quanxi
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China.
authors.forename: Weimin
authors.initials: W
authors.lastname: Ma
mesh_terms: D016825:Chlamydomonas reinhardtii / Q000187:drug effects* / Q000378:metabolism* / Q000528:radiation effects; D006859:Hydrogen / Q000378:metabolism*; D006864:Hydrogenase / Q000378:metabolism; D008027:Light; D010084:Oxidation-Reduction; D013447:Sulfites / Q000494:pharmacology*
chemical_list: D013447:Sulfites; D006859:Hydrogen; D006864:Hydrogenase; C009279:sodium bisulfite
keywords: Bisulfite photooxidation; Chlamydomonas reinhardtii; Hydrogen photoproduction; Light intensity; NaHSO3