title: Identification of microRNAs associated with glioma diagnosis and prognosis.
abstract: The sensitivity and specificity of microRNAs (miRNAs) for diagnosing glioma are controversial. We therefore performed a meta-analysis to systematically identify glioma-associated miRNAs. We initially screened five miRNA microarray datasets to evaluate the differential expression of miRNAs between glioma and normal tissues. We next compared the expression of the miRNAs in different organs and tissues to assess the sensitivity and specificity of the differentially expressed miRNAs in the diagnosis of glioma. Finally, pathway analysis was performed using GeneGO. We identified 27 candidate miRNAs associated with glioma initiation, progression, and patient prognosis. Sensitivity and specificity analysis indicated miR-15a, miR-16, miR-21, miR-23a, and miR-9 were up-regulated, while miR-124 was down-regulated in glioma. Ten signaling pathways showed the strongest association with glioma development and progression: the p53 pathway feedback loops 2, Interleukin signaling pathway, Toll receptor signaling pathway, Parkinson's disease, Notch signaling pathway, Cadherin signaling pathway, Apoptosis signaling pathway, VEGF signaling pathway, Alzheimer disease-amyloid secretase pathway, and the FGF signaling pathway. Our results indicate that the integration of miRNA, gene, and protein expression data can yield valuable biomarkers for glioma diagnosis and treatment. Indeed, six of the miRNAs identified in this study may be useful diagnostic and prognostic biomarkers in glioma.
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Xinyun
authors.initials: X
authors.lastname: Ye
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Wenjin
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Chunming
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Ruijin
authors.initials: R
authors.lastname: Yang
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Jinshi
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Zhiwu
authors.initials: Z
authors.lastname: Wu
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Qianliang
authors.initials: Q
authors.lastname: Huang
authors.affiliation: Department of Neurosurgery, The Affiliated Ganzhou Hospital of Nanchang University, Ganzhou, Jiangxi 341000, China.
authors.forename: Qiuhua
authors.initials: Q
authors.lastname: Jiang
mesh_terms: D019295:Computational Biology / Q000379:methods; D030541:Databases, Genetic; D020869:Gene Expression Profiling; D015972:Gene Expression Regulation, Neoplastic; D063990:Gene Ontology; D005910:Glioma / Q000175:diagnosis / Q000235:genetics* / Q000401:mortality; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D011237:Predictive Value of Tests; D011379:Prognosis; D034622:RNA Interference; D012333:RNA, Messenger / Q000235:genetics; D059467:Transcriptome
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger
keywords: diagnosis; glioma; meta-analysis; miRNA; prognosis