title: Inhibition of Staphylococcus aureus in vitro by bacteriocinogenic Lactococcus lactis KTH0-1S isolated from Thai fermented shrimp (Kung-som) and safety evaluation.
abstract: Lactococcus lactis KTH0-1S isolated from Thai traditional fermented shrimp (Kung-som) is able to produce heat-stable bacteriocin and inhibits food spoilage bacteria and food-borne pathogens. The inhibitory effect of bacteriocin remained intact after treatment with different pHs and after heating, but was sensitive to some proteolytic enzymes. Addition of bacteriocin KTH0-1S to Staphylococcus aureus cultures decreased viable cell counts by 2.8 log CFU/ml, demonstrating a bactericidal mode of action. Furthermore, the growth of S. aureus decreased significantly after 12-h co-cultivation with bacteriocinogenic strain. The molecular mass of bacteriocin KTH0-1S was found to be 3.346 kDa after ammonium sulfate precipitation, reversed phase (C8Sep-Pak), cation-exchange chromatography, RP-HPLC on C8column and mass spectrometry (MS/MS) analysis. Bacteriocin KTH0-1S was identified as nisin Z using PCR amplification and sequencing. The majority of tested virulence factors were absent, confirming the safety. Evidenced inhibitory effect of this strain, the absence of virulence factors creates the possibility for its application as protective culture to inhibit pathogenic bacteria in the several fermented seafood products.
authors.affiliation: Biotechnology for Bioresource Utilization Laboratory, Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat-Yai, 90112, Thailand.
authors.forename: Sutanate
authors.initials: S
authors.lastname: Saelao
authors.affiliation: Biotechnology for Bioresource Utilization Laboratory, Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat-Yai, 90112, Thailand. suppasil.m@psu.ac.th.
authors.forename: Suppasil
authors.initials: S
authors.lastname: Maneerat
authors.affiliation: Marine Natural Products Research Unit, Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat-Yai, Songkla, 90112, Thailand.
authors.forename: Sireewan
authors.initials: S
authors.lastname: Kaewsuwan
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, Équipe Fonctions et Interactions des Protéines, INRA, 44316, Nantes Cedex 03, France.
authors.forename: Hanitra
authors.initials: H
authors.lastname: Rabesona
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, Équipe Fonctions et Interactions des Protéines, INRA, 44316, Nantes Cedex 03, France.
authors.forename: Yvan
authors.initials: Y
authors.lastname: Choiset
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, Équipe Fonctions et Interactions des Protéines, INRA, 44316, Nantes Cedex 03, France.|Department of Animal Nutrition and Feed Management, Poznan University of Life Sciences, ul. Wołyńska 33, 60-637, Poznan, Poland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Haertlé
authors.affiliation: UR 1268 Biopolymères Interactions Assemblages, Équipe Fonctions et Interactions des Protéines, INRA, 44316, Nantes Cedex 03, France.
authors.forename: Jean-Marc
authors.initials: JM
authors.lastname: Chobert
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D001430:Bacteriocins / Q000737:chemistry / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D005285:Fermentation; D013294:Lactococcus lactis / Q000187:drug effects / Q000302:isolation & purification / Q000472:pathogenicity / Q000502:physiology*; D056265:Microbial Interactions; D009561:Nisin / Q000031:analogs & derivatives* / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology; D033561:Penaeidae / Q000382:microbiology; D012758:Shellfish / Q000382:microbiology; D013211:Staphylococcus aureus / Q000187:drug effects*; D013785:Thailand; D037521:Virulence Factors / Q000235:genetics
chemical_list: D000900:Anti-Bacterial Agents; D001430:Bacteriocins; D037521:Virulence Factors; D009561:Nisin; C071364:nisin Z
keywords: Inhibition; Kung-som; Lactococcus lactis; Nisin Z; Staphylococcus aureus