title: Comparative Genomics Analysis of Two Different Virulent BovinePasteurella multocidaIsolates.
abstract: ThePasteurella multocidacapsular type A isolates can cause pneumonia and bovine respiratory disease (BRD). In this study, comparative genomics analysis was carried out to identify the virulence genes in two different virulentP. multocidacapsular type A isolates (high virulent PmCQ2 and low virulent PmCQ6). The draft genome sequence of PmCQ2 is 2.32 Mbp and contains 2,002 protein-coding genes, 9 insertion sequence (IS) elements, and 1 prophage region. The draft genome sequence of PmCQ6 is 2.29 Mbp and contains 1,970 protein-coding genes, 2 IS elements, and 3 prophage regions. The genome alignment analysis revealed that the genome similarity between PmCQ2 and PmCQ6 is 99% with high colinearity. To identify the candidate genes responsible for virulence, the PmCQ2 and PmCQ6 were compared together with that of the published genomes of high virulent Pm36950 and PmHN06 and avirulent Pm3480 and Pm70 (capsular type F). Five genes and two insertion sequences are identified in high virulent strains but not in low virulent or avirulent strains. These results indicated that these genes or insertion sequences might be responsible for the virulence ofP. multocida, providing prospective candidates for further studies on the pathogenesis and the host-pathogen interactions ofP. multocida.
authors.affiliation: The State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400716, China; College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Huihui
authors.identifier: 0000-0001-7843-7868
authors.initials: H
authors.lastname: Du
authors.affiliation: College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Rendong
authors.identifier: 0000-0001-9313-3088
authors.initials: R
authors.lastname: Fang
authors.affiliation: College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Pan
authors.affiliation: The State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Tian
authors.initials: T
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Nengzhang
authors.identifier: 0000-0002-3938-2363
authors.initials: N
authors.lastname: Li
authors.affiliation: The State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Qiang
authors.identifier: 0000-0003-2535-2515
authors.initials: Q
authors.lastname: He
authors.affiliation: College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Rui
authors.initials: R
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Yuanyi
authors.identifier: 0000-0002-5572-9945
authors.initials: Y
authors.lastname: Peng
authors.affiliation: The State Key Laboratory of Silkworm Genome Biology, Southwest University, Beibei, Chongqing 400716, China.
authors.forename: Zeyang
authors.identifier: 0000-0003-2296-0742
authors.initials: Z
authors.lastname: Zhou