title: Molecular cloning and characterization of porcine ribosomal protein L21.
abstract: Ribosomal protein L21 (RPL21) is a structural component of the 60S subunit of the eukaryotic ribosome. This protein has an important role in protein synthesis and the occurrence of hereditary diseases. Pig is a common laboratory model, however, to the best of our knowledge, itsRPL21gene has not been cloned to date. In this study, we cloned and identified the full-length sequence of the pigRPL21gene for the first time. In addition, we examined its expression pattern and function by using overexpression or knockdown approaches. As a result, we obtained a 604 bp segment that contains a 483 bp open reading frame encoding 160 amino acids. The pigRPL21gene is located in the "+" strand of chromosome 11, which spans 2167 bp from 4199792 to 4201958. PigRPL21protein has nine strands and two helices in its secondary structure. PigRPL21is predominantly expressed in ovary and lung, at lower levels in kidney, small intestine, and skin, and at the lowest levels in heart and liver. Furthermore,RPL21expression is closely connected with cell proliferation and cell cycle arrest. The results are intended to provide useful information for the further study of pigRPL21.
authors.affiliation: Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.|Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Wu-Sheng
authors.initials: WS
authors.lastname: Sun
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Ju-Lan
authors.initials: JL
authors.lastname: Chun
authors.affiliation: Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
authors.forename: Dong-Hwan
authors.initials: DH
authors.lastname: Kim
authors.affiliation: Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
authors.forename: Jin-Seop
authors.initials: JS
authors.lastname: Ahn
authors.affiliation: Department of Animal Science and Biotechnology, College of Agriculture and Life Science, Chungnam National University, Daejeon 34134, Korea.
authors.forename: Min-Kyu
authors.initials: MK
authors.lastname: Kim
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Korea.
authors.forename: In-Sul
authors.initials: IS
authors.lastname: Hwang
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Korea.
authors.forename: Dae-Jin
authors.initials: DJ
authors.lastname: Kwon
authors.affiliation: Animal Biotechnology Division, National Institute of Animal Science, Wanju 55365, Korea.
authors.forename: Seongsoo
authors.initials: S
authors.lastname: Hwang
authors.affiliation: Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea.
authors.forename: Jeong-Woong
authors.initials: JW
authors.lastname: Lee
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D001483:Base Sequence; D003001:Cloning, Molecular; D005260:Female; D016366:Open Reading Frames; D010802:Phylogeny; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D012269:Ribosomal Proteins / Q000235:genetics* / Q000378:metabolism; D016415:Sequence Alignment / Q000662:veterinary; D034421:Sus scrofa / Q000235:genetics* / Q000378:metabolism
chemical_list: D012333:RNA, Messenger; D012269:Ribosomal Proteins; C060232:ribosomal protein L21
keywords: Sus scrofa; conservation; gene expression; molecular cloning; ribosomal protein L21