title: MiR-221-3p targets ARF4 and inhibits the proliferation and migration of epithelial ovarian cancer cells.
abstract: Epithelial ovarian cancer (EOC) is the most lethal gynecologic cancer. Although molecular diagnostic tools and targeted therapies have been developed over the past few decades, the survival rate is still rather low. Numerous researches suggest that some microRNAs (miRNAs) are key regulators of tumor progression. Among those miRNAs that has attracted much attention for their multiple roles in human cancers, the function of miR-221-3p in EOC has not been elucidated. Herein, we examined the expression of miR-221-3p in EOC patients and cell lines. Our data revealed that higher expression of miR-221-3p was linked to better overall survival in EOC patients. In-vitro experiments indicated that miR-221-3p inhibited EOC cell proliferation and migration. By performing subsequent systematic molecular biological and bioinformatic analyses, we found ADP-ribosylation factor (ARF) 4 is one of the putative target genes, the direct binding relationship was further confirmed by dual-luciferase reporter assay. Finally, a distinct gene expression between miR-221-3p and ARF4 in EOC group and normal group was identified, and the negative correlation between their expression levels in EOC specimens was further confirmed. Taken together, our research uncovered the tumor suppressive role of miR-221-3p in EOC and directly targeted ARF4, suggesting that miR-221-3p might be a novel potential candidate for clinical prognosis and therapeutics of EOC.
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Qihui
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Xiaolei
authors.initials: X
authors.lastname: Ren
authors.affiliation: Department of Pharmacy, Central South University, Changsha, 410013, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Xiaodan
authors.initials: X
authors.lastname: Fu
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Yimin
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Xiao
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Tong
authors.initials: T
authors.lastname: Li
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Ouyang
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Yixi
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Gynecology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China; School of Nursing, Central South University, Changsha, 410013, China.
authors.forename: Wenjuan
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China; Department of Rehabilitation, The Third Xiangya Hospital, Central South University, Changsha, 410013, China.
authors.forename: Wenguang
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China; Department of Internal Neurology, The Third Xiangya Hospital, Central South University, Changsha, 410013, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Guo
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China; Department of E.N.T., The Third Xiangya Hospital, Central South University, Changsha, 410013, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Yongbin
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Guang
authors.initials: G
authors.lastname: Shu
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Haofan
authors.initials: H
authors.lastname: Xue
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Zhangming
authors.initials: Z
authors.lastname: Wei
authors.affiliation: Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Luo
authors.affiliation: Department of Pathology, Xiangya Hospital, Central South University, Changsha, 410008, China; Department of Pathology, School of Basic Medical Sciences, Central South University, Changsha, 410013, China. Electronic address: gang.yin@csu.edu.cn.
authors.forename: Gang
authors.initials: G
authors.lastname: Yin
mesh_terms: D020727:ADP-Ribosylation Factors / Q000037:antagonists & inhibitors* / Q000235:genetics; D000328:Adult; D000077216:Carcinoma, Ovarian Epithelial; D002460:Cell Line; D002465:Cell Movement; D049109:Cell Proliferation; D005260:Female; D006801:Humans; D035683:MicroRNAs / Q000502:physiology*; D008875:Middle Aged; D009375:Neoplasms, Glandular and Epithelial / Q000188:drug therapy / Q000401:mortality / Q000473:pathology*; D010051:Ovarian Neoplasms / Q000188:drug therapy / Q000401:mortality / Q000473:pathology*; D015996:Survival Rate
chemical_list: D020727:ADP-Ribosylation Factors; D035683:MicroRNAs; C525709:MIR221, human; C116688:ARF4 protein, human
keywords: ADP-Ribosylation factor 4; Cell migration; Cell proliferation; Epithelial ovarian cancer; Overall survival; microRNA-221-3p