title: Implementation of a novel ultra fast metabolic stability analysis method using exact mass TOF-MS.
abstract: OBJECTIVE
  Increasing numbers of compounds requiring stability data means highly optimized methods capable of rapid turnaround are desirable during early discovery. Materials and methods/results: An advanced, generic analytical workflow for metabolic stability has been developed that utilizing ballistic gradient LC (sub 1 min run times), exact mass TOF-MS (Waters Xevo-G2-XS Q-TOF) and automated data processing (Waters UNIFI software) allowed for rapid integration and interpretation of all data produced, eliminating the need for method development and manual processing. We can analyze and process 96 compounds across two species in quadruplicate in a 24-h period with no method development.
  
  
  CONCLUSIONS
  An advanced bioanalytical workflow has increased our capacity threefold and reduced our instrument/processing needs threefold.
authors.affiliation: Department of Drug Metabolism & Pharmacokinetics, Celgene Corporation, 10300 Campus Point Dr., San Diego, CA 92121, USA.
authors.forename: Joseph D
authors.initials: JD
authors.lastname: Manna
authors.affiliation: Department of Drug Metabolism & Pharmacokinetics, Celgene Corporation, 10300 Campus Point Dr., San Diego, CA 92121, USA.
authors.forename: Samantha J
authors.initials: SJ
authors.lastname: Richardson
authors.affiliation: Department of Drug Metabolism & Pharmacokinetics, Celgene Corporation, 10300 Campus Point Dr., San Diego, CA 92121, USA.
authors.forename: Mehran F
authors.initials: MF
authors.lastname: Moghaddam
mesh_terms: D000818:Animals; D002626:Chemistry, Pharmaceutical / Q000379:methods*; D002851:Chromatography, High Pressure Liquid; D055808:Drug Discovery; D001330:Electronic Data Processing; D006801:Humans; D008099:Liver / Q000378:metabolism; D013058:Mass Spectrometry / Q000379:methods*; D008970:Molecular Weight; D051381:Rats; D015203:Reproducibility of Results; D012984:Software; D053719:Tandem Mass Spectrometry; D013678:Technology, Pharmaceutical; D057188:Workflow
keywords: automated data; automation; ballistic gradient; discovery processing; efficiency; high resolution; mass spectrometry; metabolic stability