title: MAMP (microbe-associated molecular pattern)-induced changes in plasma membrane-associated proteins.
abstract: Plant plasma membrane associated proteins play significant roles in Microbe-Associated Molecular Pattern (MAMP) mediated defence responses including signal transduction, membrane transport or energetic metabolism. To elucidate the dynamics of proteins associated with plasma membrane in response to cryptogein, a well-known MAMP of defence reaction secreted by the oomycete Phytophthora cryptogea, 2D-Blue Native/SDS gel electrophoresis of plasma membrane fractions was employed. This approach revealed 21 up- or down-regulated protein spots of which 15 were successfully identified as proteins related to transport through plasma membrane, vesicle trafficking, and metabolic enzymes including cytosolic NADP-malic enzyme and glutamine synthetase. Observed changes in proteins were also confirmed on transcriptional level by qRT-PCR analysis. In addition, a significantly decreased accumulation of transcripts observed after employment of a mutant variant of cryptogein Leu41Phe, exhibiting a conspicuous defect in induction of resistance, sustains the contribution of identified proteins in cryptogein-triggered cellular responses. Our data provide further evidence for dynamic MAMP-induced changes in plasma membrane associated proteins.
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czechia.
authors.forename: Hana
authors.initials: H
authors.lastname: Uhlíková
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czechia.
authors.forename: Martin
authors.initials: M
authors.lastname: Solanský
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czechia; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia.
authors.forename: Vendula
authors.initials: V
authors.lastname: Hrdinová
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czechia; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia.
authors.forename: Ondrej
authors.initials: O
authors.lastname: Šedo
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czechia.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Kašparovský
authors.affiliation: Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czechia; National Centre for Biomolecular Research, Faculty of Science, Masaryk University, Brno, Czechia.
authors.forename: Jan
authors.initials: J
authors.lastname: Hejátko
authors.affiliation: Department of Biochemistry, Faculty of Science, Masaryk University, Kotlářská 2, 61137 Brno, Czechia. Electronic address: lochik@mail.muni.cz.
authors.forename: Jan
authors.initials: J
authors.lastname: Lochman
mesh_terms: D002462:Cell Membrane / Q000378:metabolism; D002853:Chromatography, Liquid; D004591:Electrophoresis, Polyacrylamide Gel; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000378:metabolism; D010838:Phytophthora / Q000502:physiology*; D010940:Plant Proteins / Q000235:genetics / Q000378:metabolism; D053719:Tandem Mass Spectrometry; D014026:Nicotiana / Q000235:genetics* / Q000378:metabolism / Q000382:microbiology*; D014357:Trypsin / Q000737:chemistry
chemical_list: D005656:Fungal Proteins; D008565:Membrane Proteins; D000069452:Pathogen-Associated Molecular Pattern Molecules; D010940:Plant Proteins; D014357:Trypsin
keywords: ATPase; Cryptogein; Glutamine synthetase; NADP-malic enzyme; Rab8; Resistance