title: Pretreatment optimization of the biomass of Microcystis aeruginosa for efficient bioethanol production.
abstract: Microalgae are considered to be the future promising sources of biofuels and bio products. The algal carbohydrates can be fermented to bioethanol after pretreatment process. Efficient pretreatment of the biomass is one of the major requirements for commercialization of the algal based biofuels. In present study the microalga, M. aeruginsa was used for pretreatment optimization and bioethanol production. Treatment of algal biomass with CaO before acid and/or enzymatic hydrolysis enhanced the degradation of algal cells. Monomeric sugars yield was increased more than twice when biomass was pretreated with CaO. Similarly, an increase was noted in the amount of fermentable sugars when biomass was subjected to invertase saccharification after acid or lysozyme pretreatment. Highest yield of fermentable sugars (16 mM/ml) in the centrifuged algal juice was obtained. 4 Different microorganisms' species were used individually and in combination for converting centrifuged algal juice to bioethanol. Comparatively higher yield of bioethanol (60 mM/ml) was obtained when the fermenter microorganisms were used in combination. The results demonstrated that M. arginase biomass can be efficiently pretreated to get higher yield of fermentable sugars for enhanced yield of bioethanol production.
authors.affiliation: Department of Biotechnology, Chonnam Natational University, San96-1, Dun-Duk Dong, Yeosu, Chonnam, 550-749, Korea.
authors.forename: Muhammad Imran
authors.initials: MI
authors.lastname: Khan
authors.affiliation: Department of Biotechnology, Chonnam Natational University, San96-1, Dun-Duk Dong, Yeosu, Chonnam, 550-749, Korea.
authors.forename: Moon Geon
authors.initials: MG
authors.lastname: Lee
authors.affiliation: Department of Biotechnology, Chonnam Natational University, San96-1, Dun-Duk Dong, Yeosu, Chonnam, 550-749, Korea.
authors.forename: Jin Hyuk
authors.initials: JH
authors.lastname: Shin
authors.affiliation: Department of Biotechnology, Chonnam Natational University, San96-1, Dun-Duk Dong, Yeosu, Chonnam, 550-749, Korea. pasteur@jnu.ac.kr.|Research Center on Anti-Obesity and Health Care, Chonnam National University, San96-1, Dun-Duk Dong, Yeosu, Chonnam, 550-749, Korea. pasteur@jnu.ac.kr.
authors.forename: Jong Deog
authors.initials: JD
authors.lastname: Kim
keywords: Bioethanol; Fermentable sugars; Fermentation; Microcystis aeruginosa; Saccharification