title: TGFβ1-Induced LncRNA UCA1 Upregulation Promotes Gastric Cancer Invasion and Migration.
abstract: According to recent studies, long noncoding RNA urothelial carcinoma associated 1 (UCA1) is involved in the development and progression of many malignant tumors, including gastric cancer (GC). We validated the detailed role of UCA1 in human GC cell lines and GC tissues so as to determine its exact function and the underlying mechanism of GC invasion and migration. In our research, lncRNA-UCA1 was specifically upregulated in GC tissues and cell lines, and augmented GC cell proliferation, and invasive and migratory capabilities. High UCA1 expression in GC was related with poorer prognosis (poorer invasion depth, lymph node metastasis, advanced TNM [T is for the original (primary) tumor, N for nearby (regional) lymph nodes that are involved, and M for distant metastasis] stage, and shorter overall survival). Epithelial mesenchymal transition (EMT), associated with malignancy of cancers, was reported to be responsible for invasion and migration of cancer cells. Transforming growth factor β1 (TGFβ1)-induced EMT was well evaluated. UCA1 silence reduced the protein levels of EMT-related factors, vimentin and snail, while promoted E-cadherin and zonula occludens-1 protein levels in GC cells; the effect of UCA1 could be partly restored by TGFβ1 treatment. Taken together, UCA1 might regulate the tumor proliferation, invasion, and metastasis under TGFβ1 induction. Taken together, UCA1 might present a potential oncogenic factor by promoting GC cell proliferation, invasion, and migration. UCA1 could serve as a novel biomarker for prognosis and a novel therapeutic target of GC treatment.
authors.affiliation: Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University , Changsha, People's Republic of China .
authors.forename: Zhong-Kun
authors.initials: ZK
authors.lastname: Zuo
authors.affiliation: Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University , Changsha, People's Republic of China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Gong
authors.affiliation: Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University , Changsha, People's Republic of China .
authors.forename: Xiang-Heng
authors.initials: XH
authors.lastname: Chen
authors.affiliation: Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University , Changsha, People's Republic of China .
authors.forename: Fei
authors.initials: F
authors.lastname: Ye
authors.affiliation: Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University , Changsha, People's Republic of China .
authors.forename: Zheng-Ming
authors.initials: ZM
authors.lastname: Yin
authors.affiliation: Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University , Changsha, People's Republic of China .
authors.forename: Qian-Ni
authors.initials: QN
authors.lastname: Gong
authors.affiliation: Department of Minimally Invasive Surgery, The Second Xiangya Hospital, Central South University , Changsha, People's Republic of China .
authors.forename: Jiang-Sheng
authors.initials: JS
authors.lastname: Huang
mesh_terms: D000328:Adult; D015820:Cadherins / Q000235:genetics; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects; D005260:Female; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D006801:Humans; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D062085:RNA, Long Noncoding / Q000235:genetics*; D000071250:Snail Family Transcription Factors / Q000235:genetics; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology*; D016019:Survival Analysis; D053773:Transforming Growth Factor beta1 / Q000494:pharmacology*; D015854:Up-Regulation / Q000187:drug effects*; D014746:Vimentin / Q000235:genetics; D062826:Zonula Occludens-1 Protein / Q000235:genetics
chemical_list: D015820:Cadherins; D062085:RNA, Long Noncoding; D000071250:Snail Family Transcription Factors; C569042:TJP1 protein, human; D053773:Transforming Growth Factor beta1; D014746:Vimentin; D062826:Zonula Occludens-1 Protein
keywords: TGFβ1; UCA1; gastric cancer; invasion; migration