title: Enhancement of Gastric Ulcer Healing and Angiogenesis by Hepatocyte Growth Factor Gene Mediated by Attenuated Salmonella in Rats.
abstract: The present study developed an oral hepatocyte growth factor (HGF) gene therapy strategy for gastric ulcers treatment. An attenuated Salmonella typhimurium that stably expressed high HGF (named as TPH) was constructed, and the antiulcerogenic effect of TPH was evaluated in a rat model of gastric ulcers that created by acetic acid subserosal injection. From day 5 after injection, TPH (1 × 10⁹ cfu), vehicle (TP, 1 × 10⁹ cfu), or sodium bicarbonate (model control) was administered orally every alternate day for three times. Then ulcer size was measured at day 21 after ulcer induction. The ulcer area in TPH-treated group was 10.56 ± 3.30 mm², which was smaller when compared with those in the TP-treated and model control groups (43.47 ± 4.18 and 56.25 ± 6.38 mm², respectively). A higher level of reepithelialization was found in TPH-treated group and the crawling length of gastric epithelial cells was significantly longer than in the other two groups (P < 0.05). The microvessel density in the ulcer granulation tissues of the TPH-treated rats was 39.9 vessels/mm², which was greater than in the TP-treated and model control rats, with a significant statistical difference. These results suggest that TPH treatment significantly accelerates the healing of gastric ulcers via stimulating proliferation of gastric epithelial cells and enhancing angiogenesis on gastric ulcer site.
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China. haxiaoqin2013@163.com.
authors.forename: Xiaoqin
authors.identifier: http://orcid.org/0000-0002-3440-9985
authors.initials: X
authors.lastname: Ha
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Junhua
authors.identifier: http://orcid.org/0000-0001-6259-1326
authors.initials: J
authors.lastname: Peng
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Hongbin
authors.identifier: http://orcid.org/0000-0001-6439-4131
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Zhiyun
authors.identifier: http://orcid.org/0000-0003-3337-1046
authors.initials: Z
authors.lastname: Deng
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Juzi
authors.identifier: http://orcid.org/0000-0002-1925-5201
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Hongyan
authors.identifier: http://orcid.org/0000-0001-8774-0017
authors.initials: H
authors.lastname: Fan
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Yong
authors.identifier: http://orcid.org/0000-0001-9851-9035
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Bing
authors.identifier: http://orcid.org/0000-0001-8163-8329
authors.initials: B
authors.lastname: Li
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Qiangsheng
authors.identifier: http://orcid.org/0000-0003-0096-5462
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Department of Clinical Laboratory Medicine, Lanzhou General Hospital of Lanzhou Military Region, People's Liberation Army, Key Laboratory of Stem Cell and Gene Drug in Gansu Province, Lanzhou, China.
authors.forename: Zhihua
authors.identifier: http://orcid.org/0000-0002-0837-7772
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000284:Administration, Oral; D000818:Animals; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D005753:Gastric Mucosa / Q000378:metabolism / Q000473:pathology; D015316:Genetic Therapy; D017228:Hepatocyte Growth Factor / Q000032:analysis / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D008297:Male; D018919:Neovascularization, Physiologic; D019859:Proto-Oncogene Proteins c-met / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D012475:Salmonella / Q000235:genetics*; D013276:Stomach Ulcer / Q000473:pathology / Q000628:therapy*; D014945:Wound Healing
chemical_list: D017228:Hepatocyte Growth Factor; D019859:Proto-Oncogene Proteins c-met
keywords: Gastric Ulcer; Gene Therapy; Hepatocyte Growth Factor; Salmonella typhimurium; Wistar Rat