title: Antioxidant, antiglycation and insulinotrophic properties ofCoccinia grandis(L.)in vitro: Possible role in prevention of diabetic complications.
abstract: In an attempt to develop Complementary and Alternative Medicine (CAM) for the treatment of diabetes and related complications, the antidiabetic potential of the mature unripe fruits ofCoccinia grandis(CGF) was evaluated. Oxidative stress and glycation plays an important role in manifesting of diabetes and vascular complications. Agents with antioxidant and antiglycation properties may retard these pathological alterations. In this study, the edible plantCoccinia grandiswas assessed forin vitroestimation of antioxidant and antiglycation potential and its insulinotrophic properties in RINm5F cells. Antioxidant activity was evaluated as DPPH (1,1-diphenyl-2-picrylhydrazyl), hydrogen peroxide and superoxide anion scavenging activities, whereas the protein glycation inhibitory potential was evaluated usingin vitroalbumin-fructose glycation model. Glycation inhibition was estimated by different biochemical parametersviz.fructosamine, protein carbonyl group and protein aggregation using thioflavin T fluorescence.C. grandisextract exerted a dose dependent radical scavenging activity and exhibited a significant antiglycation potential. The extract also showed a significant insulinotrophic property with 1.28 and 1.71-fold increase in insulin release when compared to control at 0.25 and 0.50 mg/mL, respectively. These data suggest the possible antidiabetic role of CGF extract, presumably by its antioxidant, antiglycation and insulin secretory effects. Present findings provide experimental evidence that the fruits ofC. grandishave potential antidiabetic activity which might be used as a functional food and safe remedy for the treatment of diabetes and associated complications. This study also revealed that the plant can be a promising source for development of natural antiglycating agents and novel insulin secretagogues.
authors.affiliation: Department of Biochemistry, Bharathidasan University Constituent College for Women, Orathanadu 614 625, Thanjavur-District, Tamil Nadu, India.
authors.forename: Packirisamy
authors.initials: P
authors.lastname: Meenatchi
authors.affiliation: PG & Research Department of Biochemistry, Mohamed Sathak College of Arts and Science, Chennai 600 119, Tamil Nadu, India.
authors.forename: Ayyakkanuu
authors.initials: A
authors.lastname: Purushothaman
authors.affiliation: Department of Biochemistry, Bharathidasan University Constituent College for Women, Orathanadu 614 625, Thanjavur-District, Tamil Nadu, India.
authors.forename: Sivaprakasam
authors.initials: S
authors.lastname: Maneemegalai
keywords: Coccinia grandis; RINm5F cells; advanced glycation end products (AGEs); antiglycation; antioxidant; polyphenols