title: A proteomic approach to identify metalloproteins and metal-binding proteins in liver from diabetic rats.
abstract: Proteins play crucial roles in biological systems, thus studies comparing the protein pattern present in a healthy sample with an affected sample have been widely used for disease biomarker discovery. Although proteins containing metal ions constitute only a small proportion of the proteome, they are essential in a multitude of structural and functional processes. The correct association between metal ions and proteins is essential because this binding can significantly interfere with normal protein function. Employment of a metalloproteomic study of liver samples from diabetic rats permitted determination of the differential abundance of copper-, selenium-, zinc- and magnesium-associated proteins between diabetic, diabetic treatment with insulin and non-diabetic rats. Proteins were detected by ESI-MS/MS. Seventy-five different proteins were found with alterations in the metal ions of interest. The most prominent pathways affected under the diabetic model included: amino-acid metabolism and its derivates, glycogen storage, metabolism of carbohydrates, redox systems and glucose metabolism. Overall, the current methods employed yielded a greater understanding of metal binding and how type 1 diabetes and insulin treatment can modify some metal bonds in proteins, and therefore affect their mechanism of action and function.
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Bioscience of Botucatu, São Paulo State University, Botucatu, SP, Brazil. Electronic address: braga_ca@ibb.unesp.br.
authors.forename: Camila Pereira
authors.initials: CP
authors.lastname: Braga
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Bioscience of Botucatu, São Paulo State University, Botucatu, SP, Brazil.
authors.forename: José Cavalcante Souza
authors.initials: JCS
authors.lastname: Vieira
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: Ryan A
authors.initials: RA
authors.lastname: Grove
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: Cory H T
authors.initials: CHT
authors.lastname: Boone
authors.affiliation: Bauru Dental School, University of São Paulo- USP, Bauru, SP, Brazil.
authors.forename: Aline de Lima
authors.initials: AL
authors.lastname: Leite
authors.affiliation: Bauru Dental School, University of São Paulo- USP, Bauru, SP, Brazil.
authors.forename: Marília Afonso Rabelo
authors.initials: MAR
authors.lastname: Buzalaf
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Bioscience of Botucatu, São Paulo State University, Botucatu, SP, Brazil.
authors.forename: Ana Angélica Henrique
authors.initials: AAH
authors.lastname: Fernandes
authors.affiliation: Department of Biochemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
authors.forename: Jiri
authors.initials: J
authors.lastname: Adamec
authors.affiliation: Department of Chemistry and Biochemistry, Institute of Bioscience of Botucatu, São Paulo State University, Botucatu, SP, Brazil.
authors.forename: Pedro de Magalhaes
authors.initials: PM
authors.lastname: Padilha
mesh_terms: D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D050260:Carbohydrate Metabolism; D003920:Diabetes Mellitus / Q000378:metabolism*; D004734:Energy Metabolism; D008099:Liver / Q000378:metabolism*; D008297:Male; D008667:Metalloproteins / Q000378:metabolism*; D008670:Metals / Q000378:metabolism*; D018384:Oxidative Stress; D040901:Proteomics; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000596:Amino Acids; D008667:Metalloproteins; D008670:Metals
keywords: Electrospray ionization-tandem mass spectrometry; Flame atomic absorption spectrometry; Graphite furnace atomic absorption spectrometry; Metalloproteomic; Two-dimensional electrophoresis; Type 1 diabetes