title: Immune responses in mice vaccinated with a DNA vaccine expressing serine protease-like protein from the new-born larval stage of Trichinella spiralis.
abstract: Trichinella spiralis is a parasitic helminth that can infect almost all mammals, including humans. Trichinella spiralis infection elicits a typical type 2 immune responses, while suppresses type 1 immune responses, which is in favour of their parasitism. DNA vaccines have been shown to be capable of eliciting balanced CD4+ and CD8+ T cell responses as well as humoral immune responses in small-animal models, which will be advantage to induce protective immune response against helminth infection. In this study, serine protease (Ts-NBLsp) was encoded by a cDNA fragment of new-born T. spiralis larvae, and was inserted after CMV promoter to construct a DNA vaccine [pcDNA3·1(+)-Ts-NBLsp]. Ts-NBLsp expression was demonstrated by immunofluorescence. Sera samples were obtained from vaccinated mice, and they showed strong anti-Ts-NBLsp-specific IgG response. Mice immunized with the pcDNA3·1(+)-Ts-NBLsp DNA vaccine showed a 77·93% reduction in muscle larvae (ML) following challenge with T. spiralis ML. Our results demonstrate that the vaccination with pcDNA3·1(+)-Ts-NBLsp plasmid promoted the balance of type 1 and 2 immune responses and produced a significant protection against T. spiralis infection in mice.
authors.affiliation: Key Laboratory for Zoonoses Research,Ministry of Education,Institute of Zoonoses,Jilin University,Changchun,People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Xu
authors.affiliation: Key Laboratory for Zoonoses Research,Ministry of Education,Institute of Zoonoses,Jilin University,Changchun,People's Republic of China.
authors.forename: Xue
authors.initials: X
authors.lastname: Bai
authors.affiliation: Key Laboratory for Zoonoses Research,Ministry of Education,Institute of Zoonoses,Jilin University,Changchun,People's Republic of China.
authors.forename: Li Bo
authors.initials: LB
authors.lastname: Wang
authors.affiliation: Mucosal Immunology Laboratory,Pediatric Gastroenterology Unit,Massachusetts General Hospital East,Massachusetts,USA.
authors.forename: Hai Ning
authors.initials: HN
authors.lastname: Shi
authors.affiliation: Centre for Infectious Disease Control,National Institute for Public Health and the Environment,Amsterdam,The Netherlands.
authors.forename: Joke W B
authors.initials: JWB
authors.lastname: Van Der Giessen
authors.affiliation: Key Laboratory for Zoonoses Research,Ministry of Education,Institute of Zoonoses,Jilin University,Changchun,People's Republic of China.
authors.forename: Pascal
authors.initials: P
authors.lastname: Boireau
authors.affiliation: Key Laboratory for Zoonoses Research,Ministry of Education,Institute of Zoonoses,Jilin University,Changchun,People's Republic of China.
authors.forename: Ming Yuan
authors.initials: MY
authors.lastname: Liu
authors.affiliation: Key Laboratory for Zoonoses Research,Ministry of Education,Institute of Zoonoses,Jilin University,Changchun,People's Republic of China.
authors.forename: Xiao Lei
authors.initials: XL
authors.lastname: Liu
mesh_terms: D000818:Animals; D000909:Antibodies, Helminth / Q000096:biosynthesis / Q000097:blood; D016516:CD4-CD8 Ratio; D016207:Cytokines / Q000096:biosynthesis / Q000097:blood; D005260:Female; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D007074:Immunoglobulin G / Q000096:biosynthesis / Q000097:blood; D007814:Larva / Q000276:immunology; D051379:Mice; D010957:Plasmids / Q000235:genetics / Q000378:metabolism; D051381:Rats; D017208:Rats, Wistar; D011994:Recombinant Proteins / Q000276:immunology / Q000302:isolation & purification / Q000378:metabolism; D057057:Serine Proteases / Q000235:genetics / Q000276:immunology; D013601:T-Lymphocytes / Q000166:cytology; D017160:Trichinella spiralis / Q000276:immunology*; D014235:Trichinellosis / Q000276:immunology / Q000517:prevention & control*; D019444:Vaccines, DNA / Q000276:immunology
chemical_list: D000909:Antibodies, Helminth; D016207:Cytokines; D015801:Helminth Proteins; D007074:Immunoglobulin G; D011994:Recombinant Proteins; D019444:Vaccines, DNA; D057057:Serine Proteases
keywords: 
                 Trichinella spiralis
              ; DNA vaccine; new-born larvae; protective immunity