title: Expression of glia maturation factor γ is associated with colorectal cancer metastasis and its downregulation suppresses colorectal cancer cell migration and invasion in vitro.
abstract: Glia maturation factor γ (GMFG) functions to reorganize the actin cytoskeleton and appears to play a causative role in cell migration and adherence. The present study assessed GMFG expression in colorectal cancer cells and tissue specimens and then explored the role of GMFG in colorectal cancer progression in vitro. GMFG protein was highly expressed in colorectal cancer tissues and a metastatic colon cancer cell line. Knockdown of GMFG expression using GMFG siRNA or anti-GMFG antibody decreased the capacity of colon cancer LoVo cell migration and invasion in vitro, while recombinant GMFG treatment induced LoVo cell migration. Furthermore, GMFG knockdown also decreased expression of MMP2 protein and reversed epithelial-mesenchymal transition (EMT) phenotypes in LoVo cells. Co-culture of LoVo cells with human umbilical vein endothelial cells (HUVECs) and exogenous GMFG treatment promoted LoVo cell migration and invasion. The data from the present study indicate that GMFG should be further evaluated as a biomarker for detection of colorectal cancer metastasis and that the targeting of GMFG expression or function could be a novel strategy in the future control of colorectal cancer.
authors.affiliation: Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510000, P.R. China.
authors.forename: Huili
authors.initials: H
authors.lastname: Wang
authors.affiliation: Pediatric Center of Zhujiang Hospital, Southern Medical University, Guangzhou 510000, P.R. China.
authors.forename: Zhijiang
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Neurology, Liuzhou Hospital of Traditional Chinese Medicine, Guangxi 545001, P.R. China.
authors.forename: Hongen
authors.initials: H
authors.lastname: Chang
authors.affiliation: Guangdong Women and Children Hospital, Guangzhou 510000, P.R. China.
authors.forename: Xiaoping
authors.initials: X
authors.lastname: Mu
authors.affiliation: Guangdong Women and Children Hospital, Guangzhou 510000, P.R. China.
authors.forename: Wenyu
authors.initials: W
authors.lastname: Deng
authors.affiliation: Department of Blood Transfusion Center, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou 510000, P.R. China.
authors.forename: Zhaohu
authors.initials: Z
authors.lastname: Yuan
authors.affiliation: Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510000, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Yao
authors.affiliation: Guangdong Women and Children Hospital, Guangzhou 510000, P.R. China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Guangdong Women and Children Hospital, Guangzhou 510000, P.R. China.
authors.forename: Rongjia
authors.initials: R
authors.lastname: Mai
authors.affiliation: Research Center of Clinical Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510000, P.R. China.
authors.forename: Bingyi
authors.initials: B
authors.lastname: Wu
mesh_terms: D017209:Apoptosis; D014408:Biomarkers, Tumor / Q000378:metabolism*; D015153:Blotting, Western; D002465:Cell Movement; D049109:Cell Proliferation; D015179:Colorectal Neoplasms / Q000378:metabolism / Q000556:secondary*; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D020904:Glia Maturation Factor / Q000037:antagonists & inhibitors / Q000235:genetics / Q000378:metabolism*; D006801:Humans; D007124:Immunoenzyme Techniques; D066298:In Vitro Techniques; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009367:Neoplasm Staging; D011379:Prognosis; D012333:RNA, Messenger / Q000235:genetics; D034741:RNA, Small Interfering / Q000235:genetics; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D014407:Tumor Cells, Cultured
chemical_list: D014408:Biomarkers, Tumor; D020904:Glia Maturation Factor; D012333:RNA, Messenger; D034741:RNA, Small Interfering; C117487:glia maturation factor gamma