title: Characterization of human hybrid cell line, F2N78, through a comparison of culture performances and protein qualities.
abstract: OBJECTIVE
  To evaluate the characteristics of a novel human cell line, F2N78, including growth performance, physicochemical properties, and biological activity via direct comparison with CHO cells.
  
  
  RESULTS
  The culture performance and physicochemical properties of antibodies produced from F2N78 and CHO cells were compared. For charge variants, antibodies produced from F2N78 cells contained a greater acidic charge variants than CHO cells. Regarding main glycoforms, degree of galactosylation was 52% in CT-A produced from F2N78 cells compared to CHO cells (37%). For sialic acid forms, α-2,6-linked sialic acid and N-acetylneuraminic acid (NANA) residues were observed in antibodies produced from F2N78 cells. In contrast, only α-2,3 linked sialic acid forms were detected in antibodies produced from CHO cells, and NANA and N-glycolylneuraminic acid were detected. Hybrid structure and bisecting structure were only observed in F2N78 cells.
  
  
  CONCLUSIONS
  F2N78 cells stably produced antibodies with human specific N-glycan. The novel expression system based on human cells may facilitate the development of an alternative host cell for production of recombinant proteins.
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 22212, Korea.
authors.forename: Joon Serk
authors.initials: JS
authors.lastname: Seo
authors.affiliation: R&D Center, Celltrion, Incheon, 22014, Korea.
authors.forename: Byung Sub
authors.initials: BS
authors.lastname: Min
authors.affiliation: R&D Center, Celltrion, Incheon, 22014, Korea.
authors.forename: Yeon Jung
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: R&D Center, Celltrion, Incheon, 22014, Korea.
authors.forename: Jong Moon
authors.initials: JM
authors.lastname: Cho
authors.affiliation: R&D Center, Celltrion, Incheon, 22014, Korea.
authors.forename: Gi-Seong
authors.initials: GS
authors.lastname: Kwon
authors.affiliation: R&D Center, Celltrion, Incheon, 22014, Korea.
authors.forename: Byeong-Pil
authors.initials: BP
authors.lastname: Lim
authors.affiliation: R&D Center, Celltrion, Incheon, 22014, Korea.
authors.forename: Shin-Jae
authors.initials: SJ
authors.lastname: Chang
authors.affiliation: Department of Biological Engineering, Inha University, Incheon, 22212, Korea. kimdi@inha.ac.kr.
authors.forename: Dong-Il
authors.identifier: http://orcid.org/0000-0001-7633-382X
authors.initials: DI
authors.lastname: Kim
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis* / Q000737:chemistry; D016466:CHO Cells; D002460:Cell Line / Q000166:cytology*; D002851:Chromatography, High Pressure Liquid; D003412:Cricetulus; D006031:Glycosylation; D006801:Humans; D006822:Hybrid Cells / Q000166:cytology; D009438:Neuraminic Acids / Q000737:chemistry; D011134:Polysaccharides / Q000737:chemistry; D012794:Sialic Acids / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: D000911:Antibodies, Monoclonal; D009438:Neuraminic Acids; D011134:Polysaccharides; D012794:Sialic Acids; C032592:N-glycolylneuraminic acid
keywords: CHO cells; Charge variants; F2N78 cells; Glycan structure; Glycoform; Human cells