title: Conformational SERS Classification of K-Ras Point Mutations for Cancer Diagnostics.
abstract: Point mutations in Ras oncogenes are routinely screened for diagnostics and treatment of tumors (especially in colorectal cancer). Here, we develop an optical approach based on direct SERS coupled with chemometrics for the study of the specific conformations that single-point mutations impose on a relatively large fragment of the K-Ras gene (141 nucleobases). Results obtained offer the unambiguous classification of different mutations providing a potentially useful insight for diagnostics and treatment of cancer in a sensitive, fast, direct and inexpensive manner.
authors.affiliation: Deapartment of Physical Chemistry, Universitat Rovira i Virgili, Carrer de Marcellí Domingo s/n, 43007, Tarragona, Spain.|Medcom Advance S.A., Av. Roma, 08840, Barcelona, Spain.
authors.forename: Judit
authors.identifier: 0000-0003-1059-6939
authors.initials: J
authors.lastname: Morla-Folch
authors.affiliation: Deapartment of Physical Chemistry, Universitat Rovira i Virgili, Carrer de Marcellí Domingo s/n, 43007, Tarragona, Spain.|Medcom Advance S.A., Av. Roma, 08840, Barcelona, Spain.
authors.forename: Patricia
authors.identifier: 0000-0001-5893-1674
authors.initials: P
authors.lastname: Gisbert-Quilis
authors.affiliation: Department of Pharmacy and Biotechnology, Alma Mater Studiorum-Università di Bologna, via Belmeloro 6, 40126, Bologna, Italy.
authors.forename: Matteo
authors.identifier: 0000-0002-3757-7802
authors.initials: M
authors.lastname: Masetti
authors.affiliation: Department of Clinical Oncology, Hospital Univesitario, HM Torrelodones, 28250, Madrid, Spain.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Garcia-Rico
authors.affiliation: Deapartment of Physical Chemistry, Universitat Rovira i Virgili, Carrer de Marcellí Domingo s/n, 43007, Tarragona, Spain.|ICREA, Passeig Lluís Companys 23, 08010, Barcelona, Spain.
authors.forename: Ramon A
authors.identifier: 0000-0003-4770-5756
authors.initials: RA
authors.lastname: Alvarez-Puebla
authors.affiliation: Medcom Advance S.A., Av. Roma, 08840, Barcelona, Spain.
authors.forename: Luca
authors.identifier: 0000-0002-2925-1562
authors.initials: L
authors.lastname: Guerrini
mesh_terms: D004247:DNA / Q000235:genetics*; D004252:DNA Mutational Analysis / Q000379:methods; D011905:Genes, ras; D006801:Humans; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics; D017354:Point Mutation; D013059:Spectrum Analysis, Raman / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA; Raman spectroscopy; cancer diagnostics; gene technology; sensors