title: Increased Proangiogenic Activity of Mobilized CD34+ Progenitor Cells of Patients With Acute ST-Segment-Elevation Myocardial Infarction: Role of Differential MicroRNA-378 Expression.
abstract: OBJECTIVE
  Proangiogenic effects of mobilized bone marrow-derived stem/progenitor cells are essential for cardiac repair after myocardial infarction. MicroRNAs (miRNA/miR) are key regulators of angiogenesis. We investigated the differential regulation of angio-miRs, that is, miRNAs regulating neovascularization, in mobilized CD34+progenitor cells obtained from patients with an acute ST-segment-elevation myocardial infarction (STEMI) as compared with those with stable coronary artery disease or healthy subjects.
  
  
  RESULTS
  CD34+progenitor cells were isolated from patients with STEMI (on day 0 and day 5), stable coronary artery disease, and healthy subjects (n=27). CD34+progenitor cells of patients with STEMI exhibited increased proangiogenic activity as compared with CD34+cells from the other groups. Using a polymerase chain reaction-based miRNA-array and real-time polymerase chain reaction validation, we identified a profound upregulation of 2 known angio-miRs, that are, miR-378 and let-7b, in CD34+cells of patients with STEMI. Especially, we demonstrate that miR-378 is a critical regulator of the proangiogenic capacity of CD34+progenitor cells and its stimulatory effects on endothelial cells in vitro and in vivo, whereas let-7b upregulation in CD34+cells failed to proof its effect on endothelial cells in vivo.
  
  
  CONCLUSIONS
  The present study demonstrates a significant upregulation of the angio-miRs miR-378 and let-7b in mobilized CD34+progenitor cells of patients with STEMI. The increased proangiogenic activity of these cells in patients with STEMI and the observation that in particular miR-378 regulates the angiogenic capacity of CD34+progenitor cells in vivo suggest that this unique miRNA expression pattern represents a novel endogenous repair mechanism activated in acute myocardial infarction.
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.). Christian.Templin@usz.ch.
authors.forename: Christian
authors.initials: C
authors.lastname: Templin
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Julia
authors.initials: J
authors.lastname: Volkmann
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Maximilian Y
authors.initials: MY
authors.lastname: Emmert
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Pavani
authors.initials: P
authors.lastname: Mocharla
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Maja
authors.initials: M
authors.lastname: Müller
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Nicolle
authors.initials: N
authors.lastname: Kraenkel
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Jelena-R
authors.initials: JR
authors.lastname: Ghadri
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Martin
authors.initials: M
authors.lastname: Meyer
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Beata
authors.initials: B
authors.lastname: Styp-Rekowska
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Sylvie
authors.initials: S
authors.lastname: Briand
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Roland
authors.initials: R
authors.lastname: Klingenberg
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Milosz
authors.initials: M
authors.lastname: Jaguszewski
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Christian M
authors.initials: CM
authors.lastname: Matter
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Valentin
authors.initials: V
authors.lastname: Djonov
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Francois
authors.initials: F
authors.lastname: Mach
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Stephan
authors.initials: S
authors.lastname: Windecker
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Simon P
authors.initials: SP
authors.lastname: Hoerstrup
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Thomas
authors.initials: T
authors.lastname: Thum
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Thomas F
authors.initials: TF
authors.lastname: Lüscher
authors.affiliation: From the Department of Cardiology, University Heart Center (C.T., P.M., M.M., J.-R.G., M.J., C.M.M., T.F.L.), Department of Cardiovascular Surgery, Department of Surgical Research (M.Y.E., S.P.H.), University Hospital Zurich, Switzerland; Division of Nephrology and Hypertension, Department of Internal Medicine, Hannover Medical School, Germany (J.V.); Department of Cardiology, Campus Benjamin Franklin, Charité Universitätsmedizin Berlin, Germany (N.K., U.L.); Institute of Anatomy, University of Berne, Switzerland (B.S.-R., V.D.); Division of Cardiology, Kantonsspital Frauenfeld, Switzerland (M.M.); Division of Cardiology, Kerckhoff Klinik, Bad Nauheim, Germany (R.K.); Center for Molecular Cardiology, Schlieren Campus and Zurich Center of Integrative Human Physiology (ZIHP), University of Zurich, Switzerland (S.B., T.F.L.); First Department of Cardiology, Medical University of Gdansk, Poland (M.J.); Department of Cardiology, University of Geneva, Switzerland (F.M.); Department of Cardiology, University Hospital Bern, Switzerland (S.W.); Institute of Molecular and Translational Therapeutic Strategies (IMTTS), Hannover Medical School, Germany (T.T.); and National Heart and Lung Institute, Imperial College London, United Kingdom (T.T.).
authors.forename: Ulf
authors.initials: U
authors.lastname: Landmesser
mesh_terms: D000368:Aged; D000818:Animals; D018952:Antigens, CD34 / Q000378:metabolism*; D016022:Case-Control Studies; D002465:Cell Movement; D049109:Cell Proliferation; D002478:Cells, Cultured; D002642:Chick Embryo; D018920:Coculture Techniques; D066026:Endothelial Progenitor Cells / Q000378:metabolism* / Q000473:pathology / Q000637:transplantation; D005260:Female; D061307:Human Umbilical Vein Endothelial Cells / Q000378:metabolism; D006801:Humans; D008297:Male; D008819:Mice, Nude; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D008875:Middle Aged; D018919:Neovascularization, Physiologic; D019899:Paracrine Communication; D000072657:ST Elevation Myocardial Infarction / Q000235:genetics / Q000378:metabolism* / Q000473:pathology / Q000503:physiopathology; D015398:Signal Transduction; D013997:Time Factors; D014162:Transfection; D015854:Up-Regulation
chemical_list: D018952:Antigens, CD34; C526012:MIRN378 microRNA, human; D035683:MicroRNAs; C542687:mirnlet7 microRNA, mouse
keywords: coronary artery disease; endothelial cells; microRNAs; myocardial infarction; real-time polymerase chain reaction