title: The Time Course of Markers of Neutrophil Extracellular Traps in Patients Undergoing Revascularisation for Acute Myocardial Infarction or Stable Angina Pectoris.
abstract: Background and Aims. Neutrophil extracellular traps (NETs) have been identified in acute myocardial infarction. We assessed the time profile and association with infarct size for NETs markers in ST-elevation myocardial infarction (STEMI) and stable angina pectoris (AP).Methods.In 20 patients with STEMI and 10 with AP undergoing percutaneous coronary intervention (PCI), blood samples were collected before PCI (only AP group) and after 3 and 12 hours, days 1, 3, 5, 7, and 14 for measurement of NETs markers.Results. Double-stranded deoxyribonucleic acid (dsDNA) and nucleosome levels were higher in STEMI than AP until day 3 and 12 hours (p< 0.03, all). DsDNA declined after day 5 in both groups (p< 0.04, all), while nucleosomes declined until day 3 only in the AP group (p< 0.05, all). DsDNA correlated with peak troponin T and creatine kinase MB (CKMB) at day 5 (r= 0.48,p= 0.03, both) and with MRI-measured infarct size at days 5 and 7 (r= 0.61,p= 0.01 andr= 0.52,p= 0.04, resp.), while nucleosomes correlated with infarct size at day 5 (r= 0.58,p= 0.02).Conclusions. High levels of NETs markers were observed in STEMI shortly after revascularisation and were partly associated with infarct size. The decline thereafter in both groups indicates a role for NETs in both acute and chronic atherothrombosis.
authors.affiliation: Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, Oslo, Norway; Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Ragnhild
authors.identifier: 0000-0001-5012-9214
authors.initials: R
authors.lastname: Helseth
authors.affiliation: Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, Oslo, Norway.
authors.forename: Svein
authors.identifier: 0000-0003-3321-6572
authors.initials: S
authors.lastname: Solheim
authors.affiliation: Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, Oslo, Norway; Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Harald
authors.initials: H
authors.lastname: Arnesen
authors.affiliation: Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, Oslo, Norway; Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Ingebjørg
authors.identifier: 0000-0003-4071-3358
authors.initials: I
authors.lastname: Seljeflot
authors.affiliation: Center for Clinical Heart Research, Department of Cardiology, Oslo University Hospital Ullevål, Oslo, Norway; Faculty of Medicine, University of Oslo, Oslo, Norway.
authors.forename: Trine Baur
authors.initials: TB
authors.lastname: Opstad
mesh_terms: D000208:Acute Disease; D000368:Aged; D060050:Angina, Stable / Q000097:blood*; D015331:Cohort Studies; D052279:Creatine Kinase, MB Form / Q000097:blood; D004247:DNA / Q000737:chemistry; D065206:Extracellular Traps / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood*; D017202:Myocardial Ischemia / Q000473:pathology; D009204:Myocardial Revascularization; D009206:Myocardium / Q000473:pathology; D009707:Nucleosomes / Q000378:metabolism; D062645:Percutaneous Coronary Intervention; D013927:Thrombosis / Q000378:metabolism; D013997:Time Factors; D020107:Troponin T / Q000097:blood
chemical_list: D009707:Nucleosomes; D020107:Troponin T; D004247:DNA; D052279:Creatine Kinase, MB Form