title: Luteinizing hormone modulates intracellular calcium, protein tyrosine phosphorylation and motility during human sperm capacitation.
abstract: In order to fertilize, spermatozoa must undergo physiological and biochemical changes during their transit along the female reproductive tract before reaching and fusing with the oocyte, process known as capacitation. Sperm modifications associated with capacitation are modulated by their interaction with molecules present in the female reproductive tract. During the woman fertile window, some reproductive hormones reach their maximum concentrations in serum, such as the luteinizing hormone (LH). Since spermatozoa preparing to fertilize may be exposed to LH, the purpose of this work was to study the effects of this hormone on intracellular Ca2+concentrations ([Ca2+]i), protein tyrosine phosphorylation, sperm motility and acrosome reaction under capacitating conditions. The results showed that LH increases the duration and amplitude of Ca2+oscillations. Furthermore, motility analysis indicated that LH decreases rapid progressive motility and that sperm hyperactivation as well as several kinetic parameters augment in the presence of 0.5 and 1 μg/ml of the hormone. In addition, these two hormone concentrations also consistently promoted protein tyrosine phosphorylation. However, no effects on acrosome reaction were observed. In conclusion, the evidence indicates that LH modulates several sperm function variables involved in capacitation, suggesting that may have an important and unexplored role during human fertilization.
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Av. Vasco de Quiroga 15, Tlalpan, 14080, Mexico City, Mexico; Posgrado en Ciencias Biológicas, Universidad Nacional Autónoma de México, Av. Ciudad Universitaria 3000, Coyoacán, 04510, Mexico City, Mexico.
authors.forename: Aideé S
authors.initials: AS
authors.lastname: López-Torres
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Av. Vasco de Quiroga 15, Tlalpan, 14080, Mexico City, Mexico.
authors.forename: María E
authors.initials: ME
authors.lastname: González-González
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Cuernavaca, 62210, Mexico.
authors.forename: Esperanza
authors.initials: E
authors.lastname: Mata-Martínez
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Av. Vasco de Quiroga 15, Tlalpan, 14080, Mexico City, Mexico.
authors.forename: Fernando
authors.initials: F
authors.lastname: Larrea
authors.affiliation: Departamento de Genética del Desarrollo y Fisiología Molecular, Instituto de Biotecnología, UNAM, Cuernavaca, 62210, Mexico.
authors.forename: Claudia L
authors.initials: CL
authors.lastname: Treviño
authors.affiliation: Departamento de Biología de la Reproducción Dr. Carlos Gual Castro, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Av. Vasco de Quiroga 15, Tlalpan, 14080, Mexico City, Mexico. Electronic address: mayel.chirinose@incmnsz.mx.
authors.forename: Mayel
authors.initials: M
authors.lastname: Chirinos
mesh_terms: D020101:Acrosome Reaction / Q000187:drug effects / Q000502:physiology; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects; D005260:Female; D006801:Humans; D007986:Luteinizing Hormone / Q000378:metabolism* / Q000494:pharmacology; D008297:Male; D010766:Phosphorylation; D019000:Phosphotyrosine / Q000378:metabolism; D013075:Sperm Capacitation / Q000187:drug effects / Q000502:physiology*; D013081:Sperm Motility / Q000187:drug effects / Q000502:physiology
chemical_list: D019000:Phosphotyrosine; D007986:Luteinizing Hormone; D002118:Calcium
keywords: Capacitation; Fertilization; Luteinizing hormone; Sperm function