title: The Regulatory Roles of MicroRNA in Effects of 2,2'4,4'-Tetrabromodiphenyl Ether (BDE47) on the Transcriptome of Zebrafish Larvae.
abstract: The developmental neurotoxicity caused by environmental pollutants has received great concern; however, there were still barely known about the underlying toxic mechanisms, especially the influence of varieties of regulatory factors such as microRNA (miRNA). A representative flame retardant, 2,2',4,4'-tetrabromodiphenyl ether (BDE47), was found to disrupt zebrafish development in visual perception and bone formation in previous study, thus here we investigated its effects on miRNA expression profiling of 6 days post fertilization (dpf) zebrafish larvae by deep sequencing. To overcome the shortage of zebrafish miRNA annotation, multiple data processing approaches, especially constructed network based on the interactions between miRNAs and enrichment terms, were adopted and helped us acquire several validated zebrafish miRNAs and two novel miRNAs in BDE47-induced effects, and identify corresponding biological processes of the miRNAs. Among them, miR-735 was supposed to play essential roles in larval sensory development according to analysis results. Our study also provided an effective strategy for analyzing biological effects on non-mammalian miRNAs with limited basic information.
authors.affiliation: Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Centre of Shanghai Polytechnic University, Shanghai, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Xu
authors.affiliation: Key Laboratory of Yangtze River Water Environment, Ministry of Education, College of Environmental Science and Technology, Tongji University, Shanghai, China.
authors.forename: Daqiang
authors.initials: D
authors.lastname: Yin
authors.affiliation: Novel Bioinformatics Co., Ltd, Shanghai, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Shanghai Collaborative Innovation Centre for WEEE Recycling, WEEE Research Centre of Shanghai Polytechnic University, Shanghai, China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Bai
mesh_terms: D000818:Animals; D019295:Computational Biology / Q000379:methods; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000187:drug effects*; D053263:Gene Regulatory Networks; D055768:Halogenated Diphenyl Ethers / Q000494:pharmacology* / Q000633:toxicity; D059014:High-Throughput Nucleotide Sequencing; D035683:MicroRNAs / Q000235:genetics*; D059467:Transcriptome; D015027:Zebrafish / Q000235:genetics*
chemical_list: D055768:Halogenated Diphenyl Ethers; D035683:MicroRNAs; C511295:2,2',4,4'-tetrabromodiphenyl ether