title: Translational control of lipogenic enzymes in the cell cycle of synchronous, growing yeast cells.
abstract: Translational control during cell division determines when cells start a new cell cycle, how fast they complete it, the number of successive divisions, and how cells coordinate proliferation with available nutrients. The translational efficiencies of mRNAs in cells progressing synchronously through the mitotic cell cycle, while preserving the coupling of cell division with cell growth, remain uninvestigated. We now report comprehensive ribosome profiling of a yeast cell size series from the time of cell birth, to identify mRNAs under periodic translational control. The data reveal coordinate translational activation of mRNAs encoding lipogenic enzymes late in the cell cycle including Acc1p, the rate-limiting enzyme acetyl-CoA carboxylase. An upstream open reading frame (uORF) confers the translational control ofACC1and adjusts Acc1p protein levels in different nutrients. TheACC1uORF is relevant for cell division because its ablation delays cell cycle progression, reduces cell size, and suppresses the replicative longevity of cells lacking the Sch9p protein kinase regulator of ribosome biogenesis. These findings establish an unexpected relationship between lipogenesis and protein synthesis in mitotic cell divisions.
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.
authors.forename: Heidi M
authors.initials: HM
authors.lastname: Blank
authors.affiliation: Department of Biology, Texas A&M University, College Station, TX, USA.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Perez
authors.affiliation: The Buck Institute for Research on Aging, Novato, CA, USA.
authors.forename: Chong
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.
authors.forename: Nairita
authors.initials: N
authors.lastname: Maitra
authors.affiliation: Genomics and Bioinformatics Services, Texas A&M Agrilife Research, College Station, TX, USA.
authors.forename: Richard
authors.initials: R
authors.lastname: Metz
authors.affiliation: Genomics and Bioinformatics Services, Texas A&M Agrilife Research, College Station, TX, USA.
authors.forename: Joshua
authors.initials: J
authors.lastname: Hill
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.
authors.forename: Yuhong
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Genomics and Bioinformatics Services, Texas A&M Agrilife Research, College Station, TX, USA.
authors.forename: Charles D
authors.initials: CD
authors.lastname: Johnson
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA.|Department of Molecular & Cellular Medicine, Texas A&M Health Sciences Center, College Station, TX, USA.|Department of Chemistry, Texas A&M University, College Station, TX, USA.
authors.forename: Vytas A
authors.initials: VA
authors.lastname: Bankaitis
authors.affiliation: The Buck Institute for Research on Aging, Novato, CA, USA.
authors.forename: Brian K
authors.initials: BK
authors.lastname: Kennedy
authors.affiliation: Department of Biology, Texas A&M University, College Station, TX, USA.
authors.forename: Rodolfo
authors.initials: R
authors.lastname: Aramayo
authors.affiliation: Department of Biochemistry and Biophysics, Texas A&M University, College Station, TX, USA polymenis@tamu.edu.
authors.forename: Michael
authors.identifier: 0000-0003-1507-0936
authors.initials: M
authors.lastname: Polymenis
mesh_terms: D000103:Acetyl-CoA Carboxylase / Q000096:biosynthesis* / Q000235:genetics; D015966:Gene Expression Regulation, Fungal; D050356:Lipid Metabolism; D008938:Mitosis; D016366:Open Reading Frames; D014176:Protein Biosynthesis; D012270:Ribosomes / Q000378:metabolism; D015003:Yeasts / Q000235:genetics* / Q000254:growth & development* / Q000378:metabolism
chemical_list: D000103:Acetyl-CoA Carboxylase
keywords: 
  ACC1
  ; cell size; mitosis; translation; upstream open reading frame