title: Caspase mediated beclin-1 dependent autophagy tuning activity and apoptosis promotion by surface modified hausmannite nanoparticle.
abstract: Hidden effects of nano-materials to induced autophagy, a lysosomal degradative pathway, remain an exciting topic, in the level of material-protein interaction and subsequent cellular signaling features. Here, our studies show that surface modified hausmannite nanoparticles (Mn3O4NPs) can uniformly cleave/splice Beclin-1 protein and alter cellular mechanism on the emphasis of tuning autophagy and subsequently promote enhancement of apoptosis. Details investigation of Beclin-1 dependency and its uniform cleavage/splice pattern by surface modified Mn3O4NPs, shows tuning of cellular mechanism on emphasis of caspase mediated autophagy tuning. Our findings will also clarify the conflict between apoptosis-autophagy on the basis of its unique property derived from surface chemistry modulation, in context of Beclin-1 eminent cleavage/splice which remarks novel effect of Beclin-1 dependent tuning of autophagosomes formation and switch to enhance apoptotic index, mediates by PI3KC3 cleavage and caspase activation. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1299-1310, 2017.
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, 230027, China.|Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui, 230027, China.
authors.forename: Souvik
authors.identifier: 0000-0003-3299-9579
authors.initials: S
authors.lastname: Mondal
authors.affiliation: Department of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk, 790-784, Korea.
authors.forename: Anupam
authors.initials: A
authors.lastname: Giri
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, 230027, China.|Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui, 230027, China.
authors.forename: Yunjiao
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Chemical, Biological & Macromolecular Sciences, S. N. Bose National Centre for Basic Sciences, Block JD, Sector III, Salt Lake, Kolkata, 700098, India.
authors.forename: Samir
authors.initials: S
authors.lastname: Kumar Pal
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, 230027, China.|Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui, 230027, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: The CAS Key Laboratory of Innate Immunity and Chronic Disease, Innovation Center for Cell Signaling Network, School of Life Sciences and Medical Center, University of Science and Technology of China, Hefei, Anhui, 230027, China.|Hefei National Laboratory for Physical Sciences at Microscale, University of Science and Technology of China, Hefei, Anhui, 230027, China.
authors.forename: Long-Ping
authors.initials: LP
authors.lastname: Wen
mesh_terms: D017209:Apoptosis / Q000187:drug effects*; D001343:Autophagy / Q000187:drug effects*; D000071186:Beclin-1 / Q000737:chemistry / Q000494:pharmacology; D020169:Caspases / Q000378:metabolism*; D020099:Coated Materials, Biocompatible / Q000737:chemistry / Q000494:pharmacology; D006367:HeLa Cells; D006801:Humans; D017895:Manganese Compounds; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides
chemical_list: C491997:BECN1 protein, human; D000071186:Beclin-1; D020099:Coated Materials, Biocompatible; D017895:Manganese Compounds; D010087:Oxides; C027424:manganese oxide; D020169:Caspases
keywords: Mn3O4 nanoparticle; autophagy tuning; beclin-1 distinguished cleavage/splice; caspase activation; enhancement of apoptosis; surface chemistry modification