title: Effect of Previous High Glutamine Infusion on Inflammatory Mediators and Mortality in an Acute Pancreatitis Model.
abstract: Parenteral glutamine supplementation in acute inflammatory conditions is controversial. We evaluated the inflammatory and survival responses after parenteral glutamine infusion in sodium taurocholate-induced acute pancreatitis (AP) model. Lewis rats received 1 g/kg parenteral glutamine (n= 42), saline (n= 44), or no treatment (n= 45) for 48 h before AP induction. Blood, lung, and liver samples were collected 2, 12, and 24 h after AP to measure serum cytokines levels and tissue heat shock protein (HSP) expression. From each group, 20 animals were not sacrificed after AP for a 7-day mortality study. Serum cytokine levels did not differ among groups at any time point, but the intragroup analysis over time showed higher interferon-γonly in the nontreatment and saline groups at 2 h (versus 12 and 24 h; bothp≤ 0.05). The glutamine group exhibited greater lung and liver HSP90 expression than did the nontreatment group at 2 and 12 h, respectively; greater liver HSP90 and HSP70 expression than did the saline group at 12 h; and smaller lung HSP70 and liver HSP90 expression than did the nontreatment group at 24 h (allp≤ 0.019). The 7-day mortality rate did not differ among groups. In experimental AP, pretreatment with parenteral glutamine was safe and improved early inflammatory mediator profiles without affecting mortality.
authors.affiliation: Department of Gastroenterology, Digestive Surgery Division, University of São Paulo School of Medicine, LIM 35, São Paulo, SP, Brazil.
authors.forename: Ricardo
authors.initials: R
authors.lastname: Garib
authors.affiliation: Department of Gastroenterology, Digestive Surgery Division, University of São Paulo School of Medicine, LIM 35, São Paulo, SP, Brazil.
authors.forename: Priscila
authors.initials: P
authors.lastname: Garla
authors.affiliation: Department of Gastroenterology, Digestive Surgery Division, University of São Paulo School of Medicine, LIM 35, São Paulo, SP, Brazil; Food and Nutrition Research Center (NAPAN), University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Raquel S
authors.identifier: 0000-0003-0874-3995
authors.initials: RS
authors.lastname: Torrinhas
authors.affiliation: Department of Clinical Medicine, Clinical Emergency Laboratory, University of São Paulo School of Medicine, LIM 51, São Paulo, SP, Brazil.
authors.forename: Ana I S
authors.initials: AI
authors.lastname: Moretti
authors.affiliation: Department of Gastroenterology, Digestive Surgery Division, University of São Paulo School of Medicine, LIM 35, São Paulo, SP, Brazil.
authors.forename: Marcel C C
authors.initials: MC
authors.lastname: Machado
authors.affiliation: Department of Gastroenterology, Digestive Surgery Division, University of São Paulo School of Medicine, LIM 35, São Paulo, SP, Brazil; Food and Nutrition Research Center (NAPAN), University of São Paulo, São Paulo, SP, Brazil.
authors.forename: Dan L
authors.initials: DL
authors.lastname: Waitzberg
mesh_terms: D000208:Acute Disease; D000818:Animals; D016207:Cytokines / Q000097:blood / Q000378:metabolism; D004195:Disease Models, Animal; D005973:Glutamine / Q000008:administration & dosage*; D018840:HSP70 Heat-Shock Proteins / Q000378:metabolism; D018841:HSP90 Heat-Shock Proteins / Q000378:metabolism; D006360:Heat-Shock Proteins / Q000378:metabolism; D007249:Inflammation / Q000188:drug therapy*; D007262:Infusions, Intravenous; D007371:Interferon-gamma / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008168:Lung / Q000378:metabolism; D008297:Male; D010195:Pancreatitis / Q000188:drug therapy*; D051381:Rats; D011917:Rats, Inbred Lew; D013656:Taurocholic Acid / Q000378:metabolism; D013997:Time Factors
chemical_list: D016207:Cytokines; D018840:HSP70 Heat-Shock Proteins; D018841:HSP90 Heat-Shock Proteins; D006360:Heat-Shock Proteins; D005973:Glutamine; D013656:Taurocholic Acid; D007371:Interferon-gamma