title: Assessment of biofilm formation in Pseudomonas aeruginosa by antisense mazE-PNA.
abstract: The hallmark patogenicity in Pseudomonas aeruginosa (P. aeruginosa) is biofilm formation that is not easy to eradicate, because it has variety mechanisms for antibiotic resistance. In addition, toxin-antitoxin (TA) system may play role in biofilm formation. The current study aimed to evaluate the role of TA loci in biofilm formation. Therefore, 18 P. aeruginosa clinical isolates were collected and evaluated for specific biofilm and TA genes. The analysis by RT-qPCR demonstrated that expression of mazE antitoxin in biofilm formation was increase. On the other hand, mazE antitoxin TA system was used as target for antisense PNA. mazE-PNA was able to influence in biofilm formation and was inhibit at 5,10 and 15 μM concentrations biofilm formation in P. aeruginosa. Therefore, it could be highlighted target for anti-biofilm target to eradicate P. aeruginosa biofilm producer.
authors.affiliation: Department of Microbiology, Science and Research Branch, Islamic Azad University, Fars, Iran; Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran. Electronic address: Hasan.valadbeigi@gmail.com.
authors.forename: Hassan
authors.initials: H
authors.lastname: Valadbeigi
authors.affiliation: Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran. Electronic address: sadeghifard@gmail.com.
authors.forename: Nourkhoda
authors.initials: N
authors.lastname: Sadeghifard
authors.affiliation: Department of Microbiology, Kazeroun Branch, Islamic Azad University, Kazeroun, Iran. Electronic address: Majidbaseri@hotmail.com.
authors.forename: Majid Baseri
authors.initials: MB
authors.lastname: Salehi
mesh_terms: D000992:Antitoxins / Q000235:genetics / Q000276:immunology*; D001426:Bacterial Proteins / Q000235:genetics / Q000276:immunology*; D018441:Biofilms; D015870:Gene Expression; D006801:Humans; D007106:Immune Sera / Q000276:immunology*; D011552:Pseudomonas Infections / Q000382:microbiology; D011550:Pseudomonas aeruginosa / Q000235:genetics* / Q000276:immunology*; D040641:Quantitative Trait Loci; D013312:Stress, Physiological
chemical_list: D000992:Antitoxins; D001426:Bacterial Proteins; D007106:Immune Sera
keywords: Biofilm; P. aeruginosa; PNA; TAsystem