title: Protective Aptitude of Annexin A1 in Arterial Neointima Formation in Atherosclerosis-Prone Mice-Brief Report.
abstract: OBJECTIVE
  Restenosis as a consequence of arterial injury is aggravated by inflammatory pathways. Here, we investigate the role of the proresolving protein annexin A1 (AnxA1) in healing after wire injury.
  
  
  RESULTS
  Apoe-/-and Apoe-/-Anxa1-/-mice were subjected to wire injury while fed a high-cholesterol diet. Subsequently, localization of AnxA1 and AnxA1 plasma levels were examined. AnxA1 was found to localize within endothelial cells and macrophages in the neointima. Levels of AnxA1 in the plasma and its lesional expression negatively correlated with neointima size, and in the absence of AnxA1, neointima formation was aggravated by the accumulation and proliferation of macrophages. In contrast, reendothelialization and smooth muscle cell infiltration were not affected in Apoe-/-Anxa1-/-mice.
  
  
  CONCLUSIONS
  AnxA1 is protective in healing after wire injury and could, therefore, be an attractive therapeutic compound to prevent from restenosis after vascular damage.
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Renske J
authors.initials: RJ
authors.lastname: de Jong
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Nicole
authors.initials: N
authors.lastname: Paulin
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Patricia
authors.initials: P
authors.lastname: Lemnitzer
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Joana R
authors.initials: JR
authors.lastname: Viola
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Carla
authors.initials: C
authors.lastname: Winter
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Bartolo
authors.initials: B
authors.lastname: Ferraro
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Jochen
authors.initials: J
authors.lastname: Grommes
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Christian
authors.initials: C
authors.lastname: Weber
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Chris
authors.initials: C
authors.lastname: Reutelingsperger
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.).
authors.forename: Maik
authors.initials: M
authors.lastname: Drechsler
authors.affiliation: From the IPEK, LMU Munich, Germany (R.J.d.J., N.P., P.L., J.R.V., C. Winter, B.F., J.G., C. Weber, M.D., O.S.); Department of Pathology, AMC, Amsterdam University, The Netherlands (R.J.d.J., J.R.V., M.D., O.S.); Department of Experimental Medicine, Second University of Naples, Italy (B.F.); European Vascular Center Aachen-Maastricht, University Hospital RWTH Aachen, Germany (J.G.); Department of Biochemistry, CARIM, Maastricht University, The Netherlands (C. Weber, C.R.); and DZHK, partner site Munich Heart Alliance, Germany (C. Weber, M.D., O.S.). oliver.soehnlein@gmail.com.
authors.forename: Oliver
authors.initials: O
authors.lastname: Soehnlein
mesh_terms: D000818:Animals; D017305:Annexin A1 / Q000172:deficiency / Q000235:genetics / Q000378:metabolism*; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D002339:Carotid Arteries / Q000378:metabolism* / Q000473:pathology; D020212:Carotid Artery Injuries / Q000235:genetics / Q000378:metabolism* / Q000473:pathology; D049109:Cell Proliferation; D002478:Cells, Cultured; D059305:Diet, High-Fat; D004195:Disease Models, Animal; D042783:Endothelial Cells / Q000378:metabolism / Q000473:pathology; D020022:Genetic Predisposition to Disease; D006801:Humans; D008264:Macrophages / Q000378:metabolism / Q000473:pathology; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D058426:Neointima; D010641:Phenotype; D062326:Re-Epithelialization; D015398:Signal Transduction; D066253:Vascular Remodeling; D014945:Wound Healing
chemical_list: D017305:Annexin A1; D001057:Apolipoproteins E; C573274:annexin A1, mouse
keywords: annexin A1; macrophage; neointima formation; resolution of inflammation