title: Gene Expression Analysis of Cultured Rat-Endothelial Cells after Nd:YAG Laser Irradiation by Affymetrix GeneChip Array.
abstract: Endothelial cells and dental pulp cells enhance osteo-/odontogenic and angiogenic differentiation. In our previous study, rat pulp cells migrated to Nd:YAG laser-irradiated endothelial cells in an insert cell culture system. The purpose of this study was to examine the possible changes in the gene expression of cultured rat aortic endothelial cells after Nd:YAG laser irradiation using affymetrix GeneChip Array. Total RNA was extracted from the cells at 5 h after laser irradiation. Gene expressions were evaluated by DNA array chip. Up-regulated genes were related to cell migration and cell structure (membrane stretch, actin regulation and junctional complexes), neurotransmission and inflammation. Heat-shock 70 kDa protein (Hsp70) was related to the development of tooth germ. This study offers candidate genes for understanding the relationship between the laser-stimulated endothelial cells and dental pulp cells.
authors.affiliation: Meikai Pharmaco-Medical Laboratory (MPL), Meikai University School of Dentistry, Saitama, Japan yoshik@dent.meikai-u.ac.jp.
authors.forename: Yoshiko
authors.initials: Y
authors.lastname: Masuda
authors.affiliation: Division of Endodontics and Operative, Meikai University School of Dentistry, Saitama, Japan.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Yokose
authors.affiliation: Meikai Pharmaco-Medical Laboratory (MPL), Meikai University School of Dentistry, Saitama, Japan.|Division of Pharmacology, Meikai University School of Dentistry, Saitama, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Sakagami
mesh_terms: D000818:Animals; D001011:Aorta / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D015415:Biomarkers / Q000378:metabolism*; D002478:Cells, Cultured; D004730:Endothelium, Vascular / Q000166:cytology / Q000378:metabolism* / Q000528:radiation effects; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation / Q000528:radiation effects*; D007834:Lasers; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods*; D051381:Rats
chemical_list: D015415:Biomarkers
keywords: Affymetrix GeneChip Array; Nd:YAG laser; endothelial cell