title: A Label-free and Turn-on Fluorescence Strategy for DNA Detection with a Wide Detection Range Based on Exonuclease III-aided Target Recycling Amplification.
abstract: In the present work, a simple and sensitive turn-on fluorescence method for DNA detection was developed. It was explored based on the N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter and exonuclease III (Exo III)-aided target recycling amplification to ensure sensitivity. Our method showed an ultra-wide detection range from 10 fM to 100 nM with a low linear detection limit of 0.76 nM. It also had excellent selectivity in a selectivity experiment.
authors.affiliation: Edible and Medicinal Fungi Research Center, College of Life Science, Yangtze University.
authors.forename: Hualin
authors.initials: H
authors.lastname: Yang
authors.forename: Xiaoda
authors.initials: X
authors.lastname: Song
authors.forename: Baomiao
authors.initials: B
authors.lastname: Ding
authors.forename: Zhenshun
authors.initials: Z
authors.lastname: Li
authors.forename: Xingping
authors.initials: X
authors.lastname: Zhang
mesh_terms: D015374:Biosensing Techniques / Q000379:methods*; D004247:DNA / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism; D005090:Exodeoxyribonucleases / Q000378:metabolism*; D054856:G-Quadruplexes; D057230:Limit of Detection; D008652:Mesoporphyrins / Q000737:chemistry; D021141:Nucleic Acid Amplification Techniques; D013050:Spectrometry, Fluorescence
chemical_list: D008652:Mesoporphyrins; D004247:DNA; D005090:Exodeoxyribonucleases; C020133:exodeoxyribonuclease III