title: Data on pharmacological applications and hypothermia protection against in vitro oxygen-glucose-deprivation-related neurodegeneration of adult rat CA1 region.
abstract: In this data article, the level of chemical neuroprotection against oxygen-glucose-deprivation (OGD)-related neurodegeneration in CA1 was analyzed using the measurements on CA1 stratum pyramidale (CA1sp) width. Adult rat hippocampal slices were incubated in OGD medium for 60 min to create a model for severe ischemic conditions. Alternatively, control slices were incubated in artificial cerebrospinal fluid (ACSF) for 60 min. A study of OGD induced neurodegeneration and partial prevention by pharmacological agents reported; baclofen, memantine and l-carnitine effects were included. Also, the use of hypothermia was reported (P. Öz, H. Saybaşılı, 2016) [1]. Here, the use CA1sp width measurements on Nissl-stained hippocampal slices is introduced as a valid and affordable method for detecting the level of neurodegeneration and neuroprotection on hippocampal slices. The protective effect of hypothermia was found to be more pronounced compared to other agents.
authors.affiliation: Neuropsychopharmacology Application and Research Center, Üsküdar University Central Campus, Altunizade Mah. Haluk Türksoy Sk. No:14, 34662 Istanbul, Turkey; Institute of Biomedical Engineering Boğaziçi Üniversity Kandilli Campus, Kandilli Mah., 34684 Istanbul, Turkey.
authors.forename: Pınar
authors.initials: P
authors.lastname: Öz
authors.affiliation: Institute of Biomedical Engineering Boğaziçi Üniversity Kandilli Campus, Kandilli Mah., 34684 Istanbul, Turkey.
authors.forename: Hale
authors.initials: H
authors.lastname: Saybaşılı
keywords: Hippocampus; Histology; Oxygen-glucose-deprivation