title: Visualization of the T Cell Response in Contact Hypersensitivity.
abstract: Contact hypersensitivity (CHS) is the most basic murine model for type IV hypersensitivity. This dermatitis model is mediated by hapten-sensitized, skin-infiltrating T cells. Recent intravital imaging studies have demonstrated the dynamic migratory property of skin-infiltrating T cells and the orchestrated interaction of T cells with dermal dendritic cells. Multiphoton microscopy enables the direct, three-dimensional, and minimally invasive imaging of in vivo skin samples with high spatiotemporal resolution. Here, we describe a basic method for the intravital imaging of skin-infiltrating T cells in the DNFB-induced CHS response.
authors.affiliation: Department of Dermatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara, Kyoto, 606-8507, Japan. gyohei@kuhp.kyoto-u.ac.jp.
authors.forename: Gyohei
authors.initials: G
authors.lastname: Egawa
authors.affiliation: Department of Dermatology, Kyoto University Graduate School of Medicine, 54 Shogoin-Kawahara, Kyoto, 606-8507, Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Kabashima
mesh_terms: D000005:Abdomen; D019264:Adoptive Transfer; D000818:Animals; D017951:Antigen Presentation; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001426:Bacterial Proteins / Q000737:chemistry; D002465:Cell Movement; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D020405:Dermis / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000633:toxicity; D004423:Ear; D004817:Epidermis / Q000187:drug effects / Q000276:immunology* / Q000473:pathology; D006241:Haptens / Q000633:toxicity; D007801:Langerhans Cells / Q000187:drug effects / Q000276:immunology*; D008164:Luminescent Proteins / Q000737:chemistry; D008198:Lymph Nodes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D008807:Mice, Inbred BALB C; D036641:Microscopy, Fluorescence, Multiphoton / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods; D013601:T-Lymphocytes / Q000187:drug effects / Q000276:immunology* / Q000637:transplantation
chemical_list: D015703:Antigens, CD; D001426:Bacterial Proteins; D006241:Haptens; D008164:Luminescent Proteins; C054562:yellow fluorescent protein, Bacteria; D004139:Dinitrofluorobenzene
keywords: Adoptive transfer; Contact hypersensitivity; Dendritic cell; Hapten; Imaging; Multiphoton microscopy; T cell