title: Degradation of Di(2-Ethylhexyl) Phthalate by a Novel Gordonia alkanivorans Strain YC-RL2.
abstract: One bacterial strain, YC-RL2, isolated from petroleum-contaminated soil, could utilize environmental hormone Di(2-Ethylhexyl) phthalate (DEHP) as a sole carbon source for growth. Strain YC-RL2 was identified as Gordonia alkanivorans by 16S rRNA gene analysis and Biolog tests. The effects of environmental factors which might affect the degrading process were optimized at 30 °C and pH 8.0. Strain YC-RL2 showed superior halotolerance and could tolerate up to 0-5% NaCl in trace element medium supplemented with DEHP, although the DEHP degradation rates slowed as NaCl concentration increased. It also showed an outstanding performance in a wide range of pH (6.0-11.0). Meanwhile, strain YC-RL2 was able to withstand high concentrations of DEHP (from 100 to 800 mg/L), and the degradation rates were all above 94%. The DEHP intermediates were detected by HPLC-MS, and the degradation pathway was deduced tentatively. DEHP was transformed into phthalic acid (PA) via mono (2-ethylhexyl) phthalate (MEHP), and PA was further utilized for growth via benzoic acid (BA). The enzyme expected to catalyze the hydrolysis of MEHP to PA was identified from strain YC-RL2. Further investigation found that the enzyme could catalyze the transformation of a wide range of monoalkyl phthalates to PA. This study is the first report about species G. alkanivorans which could degrade several kinds of phthalic acid esters (PAEs), and indicates its application potential for bioremediation of PAE-polluted sites.
authors.affiliation: Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Ruth
authors.initials: R
authors.lastname: Nahurira
authors.affiliation: Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Ren
authors.affiliation: Chinese Academy of Fishery Sciences, Beijing, 100141, China.
authors.forename: Jinlong
authors.initials: J
authors.lastname: Song
authors.affiliation: Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Jia
authors.affiliation: Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Junhuan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Shuanghu
authors.initials: S
authors.lastname: Fan
authors.affiliation: Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
authors.forename: Haisheng
authors.initials: H
authors.lastname: Wang
authors.affiliation: Graduate School of Chinese Academy of Agricultural Sciences, Beijing, 100081, China. yanyanchun@caas.cn.
authors.forename: Yanchun
authors.initials: Y
authors.lastname: Yan
mesh_terms: D001673:Biodegradation, Environmental; D004051:Diethylhexyl Phthalate / Q000378:metabolism; D033601:Gordonia Bacterium / Q000378:metabolism*; D010795:Phthalic Acids / Q000378:metabolism*; D012988:Soil Microbiology
chemical_list: D010795:Phthalic Acids; C032279:phthalic acid; D004051:Diethylhexyl Phthalate