title: Polyelectrolyte pH-Responsive Protein-Containing Nanoparticles: The Physicochemical Supramolecular Approach.
abstract: We report on the physicochemical properties and self-assembly behavior of novel efficient pH-sensitive nanocontainers based on the Food and Drug Administration-approved anionic polymer Eudragit L100-55 (poly(methacrylic acid-co-ethyl acrylate) 1:1) and nonionic surfactant Brij98. The features of the interaction between Eudragit L100-55 and Brij98 at different pH values and their optimal ratio for nanoparticle formation were studied using isothermal titration calorimetry. The influence of the polymer-to-surfactant ratio on the size and structure of particles was studied at different pH values using dynamic light scattering and small-angle X-ray scattering methods. It was shown that stable nanoparticles are formed at acidic pH at polymer-to-surfactant molar ratios from 1:43 to 1:139. Trypsin was successfully encapsulated into Eudragit-Brij98 nanoparticles as a model bioactive component. The loading efficiency was determined by labeling trypsin with radioactive iodine-125. Eudragit-Brij98 nanoparticles effectively protected trypsin against pepsin digestion. The results showed that trypsin encapsulated into novel pH-sensitive nanocontainers retained more than 50% of its activity after treatment with pepsin compared with nonencapsulated trypsin. The described concept will contribute both to understanding the principles of and designing next-generation nanocontainers.
authors.affiliation: Institute of Macromolecular Chemistry , AS CR, Heyrovsky Sq. 2, Prague, Prague 6 162 06, Czech Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Riabtseva
authors.affiliation: Institute of Macromolecular Chemistry , AS CR, Heyrovsky Sq. 2, Prague, Prague 6 162 06, Czech Republic.
authors.forename: Leonid I
authors.initials: LI
authors.lastname: Kaberov
authors.affiliation: Institute of Macromolecular Chemistry , AS CR, Heyrovsky Sq. 2, Prague, Prague 6 162 06, Czech Republic.
authors.forename: Jan
authors.initials: J
authors.lastname: Kučka
authors.affiliation: Institute of Macromolecular Chemistry , AS CR, Heyrovsky Sq. 2, Prague, Prague 6 162 06, Czech Republic.
authors.forename: Anna
authors.initials: A
authors.lastname: Bogomolova
authors.affiliation: Institute of Macromolecular Chemistry , AS CR, Heyrovsky Sq. 2, Prague, Prague 6 162 06, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Stepanek
authors.affiliation: Institute of Macromolecular Chemistry , AS CR, Heyrovsky Sq. 2, Prague, Prague 6 162 06, Czech Republic.
authors.forename: Sergey K
authors.identifier: 0000-0002-4253-5076
authors.initials: SK
authors.lastname: Filippov
authors.affiliation: Institute of Macromolecular Chemistry , AS CR, Heyrovsky Sq. 2, Prague, Prague 6 162 06, Czech Republic.
authors.forename: Martin
authors.identifier: 0000-0002-5075-261X
authors.initials: M
authors.lastname: Hruby
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D000818:Animals; D002417:Cattle; D004337:Drug Carriers / Q000737:chemistry*; D000067493:Dynamic Light Scattering; D006863:Hydrogen-Ion Concentration; D007457:Iodine Radioisotopes; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D010938:Plant Oils / Q000737:chemistry*; D000071228:Polyelectrolytes / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry*; D053838:Scattering, Small Angle; D013501:Surface-Active Agents / Q000737:chemistry; D014357:Trypsin / Q000737:chemistry*; D014961:X-Ray Diffraction
chemical_list: D000180:Acrylic Resins; D004337:Drug Carriers; C446821:Eudragit L100-55; D007457:Iodine Radioisotopes; D010938:Plant Oils; D000071228:Polyelectrolytes; D013501:Surface-Active Agents; D011092:Polyethylene Glycols; C033084:polyethylene glycol oleyl ether; D014357:Trypsin