title: Impact of preformed donor-specific antibodies against HLA class I on kidney graft outcomes: Comparative analysis of exclusively anti-Cwvsanti-A and/or -B antibodies.
abstract: OBJECTIVE
  To analyze the clinical impact of preformed antiHLA-CwvsantiHLA-A and/or -B donor-specific antibodies (DSA) in kidney transplantation.
  
  
  METHODS
  Retrospective study, comparing 12 patients transplanted with DSA exclusively antiHLA-Cw with 23 patients with preformed DSA antiHLA-A and/or B.
  
  
  RESULTS
  One year after transplantation there were no differences in terms of acute rejection between the two groups (3 and 6 cases, respectively in the DSA-Cw and the DSA-A-B groups;P= 1). At one year, eGFR was not significantly different between groups (median 59 mL/min in DSA-Cw group, compared to median 51 mL/min in DSA-A-B group,P= 0.192). Moreover, kidney graft survival was similar between groups at 5-years (100% in DSA-Cw groupvs91% in DSA-A-B group,P= 0.528). The sole independent predictor of antibody mediated rejection (AMR) incidence was DSA strength (HR = 1.07 per 1000 increase in MFI,P= 0.034). AMR was associated with shortened graft survival at 5-years, with 75% and 100% grafts surviving in patients with or without AMR, respectively (Log-rankP= 0.005).
  
  
  CONCLUSIONS
  Our data indicate that DSA-Cw are associated with an identical risk of AMR and impact on graft function in comparison with "classical" class I DSA.
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Sofia
authors.initials: S
authors.lastname: Santos
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Jorge
authors.initials: J
authors.lastname: Malheiro
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Sandra
authors.initials: S
authors.lastname: Tafulo
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Leonídio
authors.initials: L
authors.lastname: Dias
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Rute
authors.initials: R
authors.lastname: Carmo
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Susana
authors.initials: S
authors.lastname: Sampaio
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Marta
authors.initials: M
authors.lastname: Costa
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Andreia
authors.initials: A
authors.lastname: Campos
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Sofia
authors.initials: S
authors.lastname: Pedroso
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Manuela
authors.initials: M
authors.lastname: Almeida
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: La Salete
authors.initials: S
authors.lastname: Martins
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: Castro
authors.initials: C
authors.lastname: Henriques
authors.affiliation: Sofia Santos, Jorge Malheiro, Leonídio Dias, Marta Costa, Andreia Campos, Sofia Pedroso, Manuela Almeida, La Salete Martins, Castro Henriques, António Cabrita, Department of Nephrology and Kidney Transplantation, Centro Hospitalar do Porto, 4099-001 Porto, Portugal.
authors.forename: António
authors.initials: A
authors.lastname: Cabrita
keywords: Anti human leukocyte antigen class I; AntiHLA-Cw antibodies; Antibody-mediated rejection; Donor-specific antibodies; Graft survival; Solid-phase immunoassays