title: Wavelength-Scanning SPR Imaging Sensors Based on an Acousto-Optic Tunable Filter and a White Light Laser.
abstract: A fast surface plasmon resonance (SPR) imaging biosensor system based on wavelength interrogation using an acousto-optic tunable filter (AOTF) and a white light laser is presented. The system combines the merits of a wide-dynamic detection range and high sensitivity offered by the spectral approach with multiplexed high-throughput data collection and a two-dimensional (2D) biosensor array. The key feature is the use of AOTF to realize wavelength scan from a white laser source and thus to achieve fast tracking of the SPR dip movement caused by target molecules binding to the sensor surface. Experimental results show that the system is capable of completing a SPR dip measurement within 0.35 s. To the best of our knowledge, this is the fastest time ever reported in the literature for imaging spectral interrogation. Based on a spectral window with a width of approximately 100 nm, a dynamic detection range and resolution of 4.63 × 10-2refractive index unit (RIU) and 1.27 × 10-6RIU achieved in a 2D-array sensor is reported here. The spectral SPR imaging sensor scheme has the capability of performing fast high-throughput detection of biomolecular interactions from 2D sensor arrays. The design has no mechanical moving parts, thus making the scheme completely solid-state.
authors.affiliation: College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen University, Shenzhen 518060, China. zyoujun@yeah.net.
authors.forename: Youjun
authors.initials: Y
authors.lastname: Zeng
authors.affiliation: College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen University, Shenzhen 518060, China. wanglei_mn@yahoo.com.
authors.forename: Lei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin 999077, Hong Kong, China. sywu@ee.cuhk.edu.hk.
authors.forename: Shu-Yuen
authors.initials: SY
authors.lastname: Wu
authors.affiliation: Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518033, China. hejianan6398@163.com.
authors.forename: Jianan
authors.initials: J
authors.lastname: He
authors.affiliation: College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen University, Shenzhen 518060, China. jlqu@szu.edu.cn.
authors.forename: Junle
authors.initials: J
authors.lastname: Qu
authors.affiliation: College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen University, Shenzhen 518060, China. lixuejin@szu.edu.cn.
authors.forename: Xuejin
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Electronic Engineering, The Chinese University of Hong Kong, Shatin 999077, Hong Kong, China. hpho@ee.cuhk.edu.hk.
authors.forename: Ho-Pui
authors.initials: HP
authors.lastname: Ho
authors.affiliation: Shenzhen Entry-exit Inspection and Quarantine Bureau, Shenzhen 518033, China. wanhood@163.com.
authors.forename: Dayong
authors.initials: D
authors.lastname: Gu
authors.affiliation: Department of Bioengineering and COMSET, Clemson University, Clemson, SC 29634, USA. zgao@clemson.edu.
authors.forename: Bruce Zhi
authors.initials: BZ
authors.lastname: Gao
authors.affiliation: College of Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, Shenzhen Key Laboratory of Sensor Technology, Shenzhen University, Shenzhen 518060, China. shaoyh@szu.edu.cn.
authors.forename: Yonghong
authors.initials: Y
authors.lastname: Shao
keywords: SPR imaging; acousto-optic tunable filter (AOTF); microarray analysis; surface plasmon resonance; wavelength scanning; white light laser