title: Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing.
abstract: Analyses of nucleic acids are routinely performed in hospital laboratories to detect gene alterations for cancer diagnosis and treatment decision. Among the different possible investigations, mRNA analysis provides information on abnormal levels of genes expression. Standard laboratory methods are still not adapted to the isolation and quantitation of low mRNA amounts and new techniques needs to be developed in particular for rare subsets analysis. By reducing the volume involved, time process, and the contamination risks, droplet microfluidics provide numerous advantages to perform analysis down to the single cell level.We report on a droplet microfluidic platform based on the manipulation of magnetic particles that allows the clinical analysis of tumor tissues. In particular, it allows the extraction of mRNA from the total-RNA sample, Reverse Transcription, and cDNA amplification, all in droplets.
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Davide
authors.initials: D
authors.lastname: Ferraro
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.|University Paris-Diderot, PRES Paris Cité, INSERM/CNRS UMR944/7212, Paris, France.|Molecular Oncology Unit, APHP, Saint-Louis Hospital, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Champ
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Bruno
authors.initials: B
authors.lastname: Teste
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: M
authors.initials: M
authors.lastname: Serra
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Malaquin
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Descroix
authors.affiliation: University Paris-Diderot, PRES Paris Cité, INSERM/CNRS UMR944/7212, Paris, France.|Molecular Oncology Unit, APHP, Saint-Louis Hospital, Paris, France.
authors.forename: Patricia
authors.initials: P
authors.lastname: de Cremoux
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France. jean-louis.viovy@curie.fr.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
mesh_terms: D014408:Biomarkers, Tumor; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis* / Q000235:genetics; D015203:Reproducibility of Results; D020133:Reverse Transcriptase Polymerase Chain Reaction; D057188:Workflow
chemical_list: D014408:Biomarkers, Tumor
keywords: Droplet microfluidics; Magnetic tweezers; PCR; Reverse Transcription (RT)