title: [Effect of triptolide on human oral cancer cell proliferation and PTEN gene mRNA expression in oral cancer].
abstract: Objective:To investigate the effect of triptolide on human oral cancer cell (HB) proliferation and phosphates and tensin homologue deleted on chromosome ten gene (PTEN) mRNA expression in oral cancer.Methods:The cancer cells were cultured in the medium containing triptolide of different concentrations for 24, 48 and 72 h. Methyl thiazolyl tetrazolium (MTT) method was used to test the rate of growth inhibition of cancer cells, flow cytometer to detect the change of cell cycle and reveres transcription-PCR (RT-PCR) to examine the expression of PTEN mRNA. The expression of PTEN protein was examined by Western blotting.Results:The rate of growth inhibition was (26.92 ± 0.14)%, (38.67 ± 0.11)%, (72.62 ± 0.89)% and (90.42 ± 0.28)%, respectively. The corresponding expression of PTEN mRNA was (3.59±0.21)%, (5.27±0.40)%, (7.18±0.44)% and (9.16±0.50)%, respectively and the correspondingAvalue of PTEN protein was 0.135±0.007, 0.410±0.020, 0.447±0.017 and 0.884±0.066, respectively. The proportion of G1 phase cells increased from (58.78±0.98)% to (84.13±0.47)%, but the proportion of S phase cells decreased from (25.40±0.43)% to (9.41±0.73)%.Conclusions:The triptolide not only had inhibitory effect on the HB proliferation, but also affected the cell cycle.
authors.affiliation: Department of Prosthodontics, School of Stomatology, North China University of Science and Technology, Tangshan 063000, China.
authors.forename: L J
authors.initials: LJ
authors.lastname: Pei
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tangshan Xiehe Hospital, Tangshan 063004, China.
authors.forename: J D
authors.initials: JD
authors.lastname: Li
authors.affiliation: Department of Prosthodontics, School of Stomatology, North China University of Science and Technology, Tangshan 063000, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Zhao
authors.affiliation: Department of Stomatology, Tangshan People's Hospital, Tangshan 063000, China.
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Prosthodontics, School of Stomatology, North China University of Science and Technology, Tangshan 063000, China.
authors.forename: R Y
authors.initials: RY
authors.lastname: Liang
authors.affiliation: Department of Oral and Maxillofacial Surgery, Tangshan Xiehe Hospital, Tangshan 063004, China.
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Xin
mesh_terms: D018906:Antineoplastic Agents, Alkylating / Q000494:pharmacology*; D017209:Apoptosis; D002453:Cell Cycle / Q000187:drug effects; D002455:Cell Division / Q000187:drug effects; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects*; D004224:Diterpenes / Q000494:pharmacology*; D004852:Epoxy Compounds / Q000494:pharmacology; D006801:Humans; D009062:Mouth Neoplasms / Q000378:metabolism* / Q000473:pathology; D051059:PTEN Phosphohydrolase / Q000235:genetics*; D010616:Phenanthrenes / Q000494:pharmacology*; D012333:RNA, Messenger / Q000378:metabolism*; D013997:Time Factors
chemical_list: D018906:Antineoplastic Agents, Alkylating; D004224:Diterpenes; D004852:Epoxy Compounds; D010616:Phenanthrenes; D012333:RNA, Messenger; C001899:triptolide; D051059:PTEN Phosphohydrolase; C494929:PTEN protein, human