title: FIP-sch2, a new fungal immunomodulatory protein from Stachybotrys chlorohalonata, suppresses proliferation and migration in lung cancer cells.
abstract: Fungal immunomodulatory protein (FIP)-sch2, an immunomodulatory protein identified in the ascomycete Stachybotrys chlorohalonata by a sequence similarity search, is a novel member of the FIP family. FIP-sch2 shares high sequence identity, structure, and evolutionary conservation with previously reported FIPs. It was satisfactorily expressed in Escherichia coli with a glutathione S-transferase (GST) tag and purified by GST-affinity magnetic beads. To characterize the direct antitumor effects, human lung adenocarcinoma A549 cells were treated with different concentrations of recombinant FIP (rFIP)-sch2 in vitro, and the results showed that rFIP-sch2 could reduce cell viability dose-dependently with a half-maximal inhibitory concentration (IC50) of 9.48 μg/mL. Furthermore, rFIP-sch2 at 8 μg/mL could significantly induce apoptosis and interrupt migration in A549 cells. Notably, the antitumor effect of rFIP-sch2 was equivalent to that of rLZ-8 but was obviously increased compared to rFIP-fve. In addition, the exploration of the antitumor mechanism suggested that rFIP-sch2 induced lung cancer cell death by activating apoptosis and inhibiting migration. Our results indicated that rFIP-sch2 was a promising candidate for use in future cancer therapy.
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Shuying
authors.initials: S
authors.lastname: Li
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Zhonghao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Xu
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Xie
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Leiming
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Tang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. wangfengzhong@sina.com.
authors.forename: Fengzhong
authors.initials: F
authors.lastname: Wang
authors.affiliation: Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. 2002hongzhi30@163.com.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Xin
mesh_terms: D000072283:A549 Cells; D017209:Apoptosis / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects*; D002470:Cell Survival / Q000187:drug effects; D004926:Escherichia coli / Q000235:genetics; D005656:Fungal Proteins / Q000235:genetics / Q000302:isolation & purification / Q000494:pharmacology*; D005982:Glutathione Transferase / Q000235:genetics; D006801:Humans; D056747:Immunomodulation; D008175:Lung Neoplasms / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D011994:Recombinant Proteins / Q000302:isolation & purification / Q000494:pharmacology; D016415:Sequence Alignment; D013191:Stachybotrys / Q000737:chemistry* / Q000235:genetics / Q000302:isolation & purification
chemical_list: D005656:Fungal Proteins; D011994:Recombinant Proteins; D005982:Glutathione Transferase
keywords: Apoptosis; Fungal immunomodulatory protein; Migration; Proliferation