title: Enzyme-responsive reporter molecules for selective localization and fluorescence imaging of pathogenic biofilms.
abstract: Pathogenic bacteria and their biofilm formation are responsible for a broad spectrum of microbial infections. A novel enzyme-responsive reporter molecule (ERM-1), which can specifically recognize AmpC β-lactamase (Bla) in drug resistant bacteria, has been developed to enable the selective localization of biofilms.
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore. bengang@ntu.edu.sg.
authors.forename: Junxin
authors.initials: J
authors.lastname: Aw
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore. bengang@ntu.edu.sg.
authors.forename: Frances
authors.identifier: 0000-0002-6258-4799
authors.initials: F
authors.lastname: Widjaja
authors.affiliation: Centre for Environmental Life Sciences Engineering (SCELSE), School of Biological Sciences, Nanyang Technological University, Singapore, 637551, Singapore and Interdisciplinary Graduate School (IGS), Nanyang Technological University, 639798, Singapore.
authors.forename: Yichen
authors.initials: Y
authors.lastname: Ding
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore. bengang@ntu.edu.sg.
authors.forename: Jing
authors.initials: J
authors.lastname: Mu
authors.forename: Liang
authors.lastname: Yang
authors.affiliation: Division of Chemistry and Biological Chemistry, School of Physical & Mathematical Sciences, Nanyang Technological University, Singapore, 637371, Singapore. bengang@ntu.edu.sg and Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), Singapore, 117602, Singapore.
authors.forename: Bengang
authors.identifier: 0000-0002-8391-1234
authors.initials: B
authors.lastname: Xing
mesh_terms: D001426:Bacterial Proteins / Q000737:chemistry; D001559:Benzhydryl Compounds / Q000737:chemistry*; D018441:Biofilms / Q000254:growth & development*; D002511:Cephalosporins / Q000737:chemistry*; D024881:Drug Resistance, Bacterial; D004755:Enterobacteriaceae / Q000502:physiology*; D005456:Fluorescent Dyes / Q000737:chemistry*; D006868:Hydrolysis; D018613:Microscopy, Confocal / Q000379:methods; D008856:Microscopy, Fluorescence / Q000379:methods; D057054:Molecular Imaging; D001618:beta-Lactamases / Q000737:chemistry
chemical_list: D001426:Bacterial Proteins; D001559:Benzhydryl Compounds; D002511:Cephalosporins; D005456:Fluorescent Dyes; C048795:AmpC beta-lactamases; D001618:beta-Lactamases; C034413:beta-lactamase TEM-1