title: Monitoring and Modulation of Inducible Foxp3+Regulatory T-Cell Differentiation in the Lymph Nodes Draining the Small Intestine and Colon.
abstract: The mucosa-draining lymphoid tissue favors differentiation of inducible Foxp3+regulatory T cells. Adoptive transfer of T-cell receptor (TCR) transgenic (Tg) T cells is a powerful tool to study antigen-specific regulatory T-cell differentiation in lymphoid tissues in vivo. The kinetics and nature of the T-cell response largely depend on the route of antigen administration and degree of clonal competition. Here, we describe that adoptive transfer of CD4+DO11.10 TCR Tg T cells can be used for monitoring Foxp3+regulatory T-cell differentiation in the gut-draining lymph nodes. We describe two routes of mucosal antigen administration, e.g., the oral and intracolonic route known to induce T-cell responses in the small intestine-draining mesenteric lymph nodes (MLN) and distal colon-draining caudal and iliac lymph nodes (ILN), respectively. In particular, we discuss differences in frequency of inducible Foxp3+regulatory T cells after adoptive transfer of variable numbers of Tg T cells and various amounts of orally gavaged ovalbumin (OVA), and explain how Foxp3+regulatory T-cell differentiation can be modulated by coadministration of the adjuvant cholera toxin (CT) with OVA using this adoptive transfer system.
authors.affiliation: Laboratory of Pediatrics, Division Gastroenterology and Nutrition, Erasmus University Medical Center, Room Ee1567A, 2040, 3000, CA, Rotterdam, The Netherlands.
authors.forename: S
authors.initials: S
authors.lastname: Veenbergen
authors.affiliation: Laboratory of Pediatrics, Division Gastroenterology and Nutrition, Erasmus University Medical Center, Room Ee1567A, 2040, 3000, CA, Rotterdam, The Netherlands.
authors.forename: L A
authors.initials: LA
authors.lastname: van Berkel
authors.affiliation: Laboratory of Pediatrics, Division Gastroenterology and Nutrition, Erasmus University Medical Center, Room Ee1567A, 2040, 3000, CA, Rotterdam, The Netherlands.
authors.forename: M F
authors.initials: MF
authors.lastname: du Pré
authors.affiliation: Laboratory of Pediatrics, Division Gastroenterology and Nutrition, Erasmus University Medical Center, Room Ee1567A, 2040, 3000, CA, Rotterdam, The Netherlands.
authors.forename: A E
authors.initials: AE
authors.lastname: Kozijn
authors.affiliation: Laboratory of Pediatrics, Division Gastroenterology and Nutrition, Erasmus University Medical Center, Room Ee1567A, 2040, 3000, CA, Rotterdam, The Netherlands. j.samsom@erasmusmc.nl.
authors.forename: Janneke N
authors.initials: JN
authors.lastname: Samsom
mesh_terms: D000276:Adjuvants, Immunologic / Q000008:administration & dosage; D019264:Adoptive Transfer / Q000379:methods*; D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002454:Cell Differentiation / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D002772:Cholera Toxin / Q000008:administration & dosage; D003106:Colon / Q000166:cytology* / Q000276:immunology; D005434:Flow Cytometry; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D018928:Immunity, Mucosal; D007421:Intestine, Small / Q000166:cytology* / Q000276:immunology; D008198:Lymph Nodes / Q000166:cytology* / Q000276:immunology; D051379:Mice; D008807:Mice, Inbred BALB C; D008822:Mice, Transgenic; D010047:Ovalbumin / Q000008:administration & dosage; D011948:Receptors, Antigen, T-Cell / Q000235:genetics / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods*; D050378:T-Lymphocytes, Regulatory / Q000166:cytology* / Q000276:immunology / Q000637:transplantation
chemical_list: D000276:Adjuvants, Immunologic; D015415:Biomarkers; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D011948:Receptors, Antigen, T-Cell; D010047:Ovalbumin; D002772:Cholera Toxin
keywords: Adoptive transfer; Clonal competition; Foxp3; Mucosal immune regulation; OT-II TCR transgenic; Small intestine and colon; Tregs; iTregs