title: Therapeutic Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Acute Lung Injury Mice.
abstract: The incidence and mortality of acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) are still very high, but stem cells show some promise for its treatment. Here we found that intratracheal administration of human umbilical cord-mesenchymal stem cells (UC-MSCs) significantly improved survival and attenuated the lung inflammation in lipopolysaccharide (LPS)-induced ALI mice. We also used the proteins-chip and bioinformatics to analyze interactions between UC-MSCs treatment and immune-response alternations of ALI mice. Then we demonstrated that UC-MSCs could inhibit the inflammatory response of mouse macrophage in ALI mice, as well as enhance its IL-10 expression. We provide data to support the concept that the therapeutic capacity of UC-MSCs for ALI was primarily through paracrine secretion, particularly of prostaglandin-E2 (PGE2). Furthermore, we showed that UC-MSCs might secrete a panel of factors including GM-CSF, IL-6 and IL-13 to ameliorate ALI. Our study suggested that UC-MSCs could protect LPS-induced ALI model by immune regulation and paracrine factors, indicating that UC-MSCs should be a promising strategy for ALI/ARDS.
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Pediatrics, First Affiliated Hospital of China Medical University, Shenyang 110001, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Yunqiu
authors.initials: Y
authors.lastname: Xia
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Rong
authors.initials: R
authors.lastname: Zhang
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Daiyin
authors.initials: D
authors.lastname: Tian
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Wang
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Jihong
authors.initials: J
authors.lastname: Dai
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Lijia
authors.initials: L
authors.lastname: Wang
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Otorhinolaryngology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Hongbing
authors.initials: H
authors.lastname: Yao
authors.affiliation: Department of Pediatrics, First Affiliated Hospital of China Medical University, Shenyang 110001, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jiang
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Chongqing Engineering Research Center of Stem Cell Therapy, Chongqing 400014, China.
authors.forename: Ke
authors.initials: K
authors.lastname: Yang
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Enmei
authors.initials: E
authors.lastname: Liu
authors.affiliation: Laboratoryof Pathology, West China Hospital, Sichuan University, Chengdu 610041, China.
authors.forename: Yujun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Respiratory Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Chongqing Engineering Research Center of Stem Cell Therapy, Chongqing 400014, China.
authors.forename: Zhou
authors.initials: Z
authors.lastname: Fu
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Department of Otorhinolaryngology, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Chongqing Engineering Research Center of Stem Cell Therapy, Chongqing 400014, China.
authors.forename: Li
authors.initials: L
authors.lastname: Gao
authors.affiliation: Pediatrics Research Institute, Ministry of Education Key Laboratory of Child Development and Disorders, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.|Chongqing Engineering Research Center of Stem Cell Therapy, Chongqing 400014, China.|Center for Clinical Molecular Medicine, Children's Hospital of Chongqing Medical University, Chongqing 400014, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zou
mesh_terms: D055371:Acute Lung Injury / Q000209:etiology / Q000401:mortality / Q000628:therapy*; D000818:Animals; D001992:Bronchoalveolar Lavage Fluid / Q000737:chemistry; D002478:Cells, Cultured; D015232:Dinoprostone / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000378:metabolism; D006801:Humans; D016753:Interleukin-10 / Q000378:metabolism; D008070:Lipopolysaccharides / Q000633:toxicity; D008168:Lung / Q000378:metabolism / Q000473:pathology; D008264:Macrophages / Q000166:cytology / Q000378:metabolism; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells / Q000166:cytology* / Q000378:metabolism; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D015996:Survival Rate; D014470:Umbilical Cord / Q000166:cytology
chemical_list: D008070:Lipopolysaccharides; D016753:Interleukin-10; D016178:Granulocyte-Macrophage Colony-Stimulating Factor; D015232:Dinoprostone