title: Eradication of melanoma in vitro and in vivo via targeting with a Killer-Red-containing telomerase-dependent adenovirus.
abstract: Melanoma is a highly recalcitrant cancer and transformative therapy is necessary for the cure of this disease. We recently developed a telomerase-dependent adenovirus containing the fluorescent protein Killer-Red. In the present report, we first determined the efficacy of Killer-Red adenovirus combined with laser irradiation on human melanoma cell lines in vitro. Cell viability of human melanoma cells was reduced in a dose-dependent and irradiation-time-dependent manner. We used an intradermal xenografted melanoma model in nude mice to determine efficacy of the Killer-Red adenovirus. Intratumoral injection of Killer-Red adenovirus, combined with laser irradiation, eradicated the melanoma indicating the potential of a new paradigm of cancer therapy.
authors.affiliation: a AntiCancer, Inc. , San Diego , CA , USA.|b Department of Surgery , University of California San Diego , San Diego , CA , USA.|c Department of Gastroenterological Surgery , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Kiyoto
authors.initials: K
authors.lastname: Takehara
authors.affiliation: a AntiCancer, Inc. , San Diego , CA , USA.|b Department of Surgery , University of California San Diego , San Diego , CA , USA.|c Department of Gastroenterological Surgery , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Shuya
authors.initials: S
authors.lastname: Yano
authors.affiliation: d Center for Innovative Clinical Medicine , Okayama University Hospital , Okayama , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Tazawa
authors.affiliation: c Department of Gastroenterological Surgery , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Kishimoto
authors.affiliation: e Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences , Osaka University , Osaka , Japan.
authors.forename: Nobuhiro
authors.initials: N
authors.lastname: Narii
authors.affiliation: e Laboratory of Biochemistry and Molecular Biology, Graduate School of Pharmaceutical Sciences , Osaka University , Osaka , Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Mizuguchi
authors.affiliation: f Oncolys BioPharma, Inc. , Tokyo , Japan.
authors.forename: Yasuo
authors.initials: Y
authors.lastname: Urata
authors.affiliation: c Department of Gastroenterological Surgery , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Shunsuke
authors.initials: S
authors.lastname: Kagawa
authors.affiliation: c Department of Gastroenterological Surgery , Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Toshiyoshi
authors.initials: T
authors.lastname: Fujiwara
authors.affiliation: a AntiCancer, Inc. , San Diego , CA , USA.|b Department of Surgery , University of California San Diego , San Diego , CA , USA.
authors.forename: Robert M
authors.initials: RM
authors.lastname: Hoffman
mesh_terms: D000256:Adenoviridae / Q000378:metabolism*; D000818:Animals; D045744:Cell Line, Tumor; D002470:Cell Survival / Q000528:radiation effects; D049452:Green Fluorescent Proteins / Q000378:metabolism*; D006801:Humans; D008027:Light; D008545:Melanoma / Q000473:pathology* / Q000532:radiotherapy; D008819:Mice, Nude; D010778:Photochemotherapy; D019098:Telomerase / Q000378:metabolism*; D013997:Time Factors
chemical_list: C508771:killer red protein, Anthomedusae; D049452:Green Fluorescent Proteins; D019098:Telomerase
keywords: adenovirus; killer-red; melanoma; nude mouse; photodynamic therapy; telomerase