title: Newcastle disease virus vectored infectious laryngotracheitis vaccines protect commercial broiler chickens in the presence of maternally derived antibodies.
abstract: Newcastle disease virus (NDV) recombinants expressing the infectious laryngotracheitis virus (ILTV) glycoproteins B and D have previously been demonstrated to confer complete clinical protection against virulent ILTV and NDV challenges in naive chickens. We extended this study to assess whether maternally derived antibody (MDA) against NDV and ILTV would interfere with protection in vaccinated broiler chickens. Chickens with a mean NDV MDA hemagglutination inhibition (HI) titer of 6.4 (log2) and detectable ILTV neutralization (VN) antibodies at hatch were vaccinated with rLS/ILTV-gB or rLS/ILTV-gD at 1 or 10day of age (DOA) or on both days. Groups of birds vaccinated with the commercial ILT vaccines (FP-LT and CEO) or sham inoculated were also included in this study. All vaccinated birds were challenged with virulent ILTV strain at 21 DOA. By that time, NDV HI titers declined to 2.6 (log2) in unvaccinated birds, whereas the HI titers in NDV vectored vaccine groups increased to 3.5-6.3 (log2). At standard dosages, both vaccine candidates conferred significant clinical protection; however, the protection elicited by the rLS/ILTV-gD was superior to that of rLS/ILTV-gB. Recombinant rLS/ILTV-gD reduced ILTV shedding from tracheal and ocular tissues by approximately 3 log10TCID50. Notably, there was no improvement in protection after booster vaccination at 10 DOA. Overall results indicate that the presence of maternal antibodies to NDV and ILTV did not significantly interfere with the ability of the NDV LaSota strain-vectored ILTV gB and gD vaccine candidates to elicit protective immunity against infectious laryngotracheitis.
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA. Electronic address: qingzhong.yu@ars.usda.gov.
authors.forename: Qingzhong
authors.initials: Q
authors.lastname: Yu
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Stephen
authors.initials: S
authors.lastname: Spatz
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Yufeng
authors.initials: Y
authors.lastname: Li
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Jilong
authors.initials: J
authors.lastname: Yang
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhao
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Guoyuan
authors.initials: G
authors.lastname: Wen
authors.affiliation: Poultry Diagnostic and Research Center, Department of Population Health, College of Veterinary Medicine, The University of Georgia, 953 College Station Road, Athens, GA 30602, USA.
authors.forename: Maricarmen
authors.initials: M
authors.lastname: Garcia
authors.affiliation: US National Poultry Research Center, Southeast Poultry Research Laboratory, Agricultural Research Services, United States Department of Agriculture, 934 College Station Road, Athens, GA 30605, USA.
authors.forename: Laszlo
authors.initials: L
authors.lastname: Zsak
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000096:biosynthesis*; D000914:Antibodies, Viral / Q000096:biosynthesis*; D002645:Chickens / Q000821:virology; D015870:Gene Expression; D006385:Hemagglutination Inhibition Tests; D006566:Herpesviridae Infections / Q000276:immunology / Q000517:prevention & control / Q000662:veterinary* / Q000821:virology; D001352:Herpesvirus 1, Gallid / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D007117:Immunization, Secondary; D000072741:Maternal Inheritance; D009521:Newcastle Disease / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D009522:Newcastle disease virus / Q000187:drug effects / Q000235:genetics / Q000276:immunology; D011201:Poultry Diseases / Q000276:immunology / Q000517:prevention & control* / Q000821:virology; D011994:Recombinant Proteins / Q000235:genetics / Q000276:immunology; D013997:Time Factors; D014611:Vaccination; D064166:Vaccine Potency; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000096:biosynthesis / Q000235:genetics; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000096:biosynthesis / Q000235:genetics
chemical_list: D057134:Antibodies, Neutralizing; D000914:Antibodies, Viral; D011994:Recombinant Proteins; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C000591972:glycoprotein B, Gallid herpesvirus 1; C094674:glycoprotein D, Gallid herpesvirus 1
keywords: ILTV; Interference; Maternal antibody; NDV; Protection; Recombinant vaccine