title: Chemical input reduction in the arabinoxylan and lignocellulose alkaline extraction and xylooligosaccharides production.
abstract: Lignocellulosic material breakdown by hydrolysis is an important step to open new perspectives for bioenergy and special foods production like prebiotic xylooligosaccharides. Improvement of lignocellulose and arabinoxylan alkaline extraction from sugarcane bagasse and enzymatic hydrolysis were performed. Treatments 1 (10% KOH at 70°C), 3 (5% KOH at 121°C) and ZD method (24% KOH at 35°C) showed solid lignocellulose recovery of respectively 75.2%, 74.2% and 73%. A range of 24.8-27% extracted material with high arabinoxylan content (72.1-76.3%) was obtained with these treatments. Treatment 1 and 3 exhibited great KOH reduction in the method reaction, 54.1% and 76.2%, respectively. Likewise, in treatment 3 there was a decrease in ethanol consumption (40.9%) when compared to ZD method. The extracted arabinoxylan showed susceptibility to enzymatic hydrolysis with high solid loading (7%) since Trichoderma reesei xylanases were advantageous for xylose production (54.9%), while Aspergillus fumigatus xylanases achieved better XOS production (27.1%).
authors.affiliation: Departament of Biochemistry and Microbiology, Institute of Biosciences, São Paulo State University (UNESP), Avenida 24 A, 1515, ZIP Code 13506-900 Rio Claro, SP, Brazil; Laboratory of Development of Bioprocesses, Bioenergy Research Institute (IPBEN), School of Sciences and Languages, São Paulo State University (UNESP), Avenida Dom Antonio, 2100, ZIP Code 19806-900 Assis, SP, Brazil. Electronic address: franciane.c.figueiredo@gmail.com.
authors.forename: Franciane Cristina
authors.initials: FC
authors.lastname: de Figueiredo
authors.affiliation: Laboratory of Development of Bioprocesses, Bioenergy Research Institute (IPBEN), School of Sciences and Languages, São Paulo State University (UNESP), Avenida Dom Antonio, 2100, ZIP Code 19806-900 Assis, SP, Brazil.
authors.forename: Ana Flavia Azevedo
authors.initials: AFA
authors.lastname: Carvalho
authors.affiliation: Laboratory of Biomass Characterization, Bioenergy Research Institute (IPBEN), São Paulo State University (UNESP), Avenida 24 A, 1515, ZIP Code 13506-900 Rio Claro, SP, Brazil.
authors.forename: Michel
authors.initials: M
authors.lastname: Brienzo
authors.affiliation: Laboratory of Development of Bioprocesses, Bioenergy Research Institute (IPBEN), School of Sciences and Languages, São Paulo State University (UNESP), Avenida Dom Antonio, 2100, ZIP Code 19806-900 Assis, SP, Brazil.
authors.forename: Tania Sila
authors.initials: TS
authors.lastname: Campioni
authors.affiliation: Laboratory of Development of Bioprocesses, Bioenergy Research Institute (IPBEN), School of Sciences and Languages, São Paulo State University (UNESP), Avenida Dom Antonio, 2100, ZIP Code 19806-900 Assis, SP, Brazil.
authors.forename: Pedro
authors.initials: P
authors.lastname: de Oliva-Neto
mesh_terms: D019149:Bioreactors; D002482:Cellulose / Q000737:chemistry*; D005965:Glucuronates / Q000138:chemical synthesis* / Q000737:chemistry; D006868:Hydrolysis; D008031:Lignin / Q000737:chemistry* / Q000378:metabolism; D009844:Oligosaccharides / Q000138:chemical synthesis*; D031786:Saccharum / Q000737:chemistry; D014865:Waste Disposal, Fluid / Q000379:methods*; D014990:Xylans / Q000737:chemistry*; D014994:Xylose / Q000378:metabolism
chemical_list: D005965:Glucuronates; D009844:Oligosaccharides; D014990:Xylans; C570991:xylooligosaccharide; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; C085118:arabinoxylan; D014994:Xylose
keywords: Alkaline treatment; Enzymatic hydrolysis; Lignocellulose; Sugarcane bagasse; Xylooligosaccharides