title: Evaluation of Different Primers for Detection ofBrucellaby Using PCR Method.
abstract: BACKGROUND
  Brucellosis is a worldwide zoonosis and a significant cause of loss of health in humans and animals. Traditionally, classic diagnosis is carried out by isolation ofBrucella, which is time-consuming, technically challenging and potentially dangerous. The aim of this study was to expand a molecular test that would be used for the develop detection ofBrucellain a single reaction with high sensitivity and specificity, by targeting IS711element.
  
  
  METHODS
  This study was carried out from 2015 to 2016 at the Ayatolla Rohani hospital in Babol, Iran. The present study was designed to develop PCR assay, based on IS711 gene for rapid diagnosis ofBrucella spp. and immediate detection ofBrucella, with high sensitivity and specificity. Four pairs of oligo-nucleotide primers with sizes of 547, 403, 291 and 127bp respectively, were planned to exclusively amplify the targeted genes of Brucella species.
  
  
  RESULTS
  Our results show that, five PCR primers set up, would be helpful in amplifying the DNAs from the genusBrucellawith high specificity and sensitivity so it can be 12 fg, forBrucellaspecies to provide a valuable tool for diagnosis.
  
  
  CONCLUSIONS
  This method can be more useful than serological and biochemical tests and in addition, this reduces the number of required tests more rapidly and economically.
authors.affiliation: Ph.D. in Biomedical Sciences, Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Moulana
authors.affiliation: Professor of Infectious Diseases, Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
authors.forename: Mohammad Reza Hasanjani
authors.initials: MR
authors.lastname: Roushan
authors.affiliation: Assistant Professor in Medical Bacteriology Department of Microbiology and Immunology Alborz University of medical sciences, Karaj, Iran.
authors.forename: Seyed Mahmoud Amin
authors.initials: SM
authors.lastname: Marashi
keywords: Brucellosis; Diagnosis; IS711gene; Polymerase chain reaction