title: Association of ANRIL gene polymorphisms with major adverse cardiovascular events in hemodialysis patients.
abstract: BACKGROUND
  Cardiovascular disease is the leading cause of death in patients with chronic kidney disease (CKD). Single nucleotide polymorphisms (SNPs) in ANRIL gene have been associated with higher cardiovascular morbidity and mortality in general population. The main objective was to ascertain whether ANRIL polymorphisms could identify risk of major adverse cardiovascular event (MACE) in patients starting on hemodialysis (HD).
  
  
  METHODS
  This was a prospective observational cohort study. 284 CKD patients starting on HD were included in the study and followed until achievement of the primary end-point (MACE) or end of the study. All patients were genotyped for four ANRIL SNPs (rs10757278, rs4977574, rs10757274 and rs6475606). Kaplan-Meier curves and multivariate Cox survival analyses, together with multiple logistic regression were used to analyze the association between ANRIL SNPs and MACE.
  
  
  RESULTS
  We found that ANRIL SNP rs10757278 was a representative SNP of a strong linkage disequilibrium block and showed significant genotypic associations with MACE in hemodialysis patients. Homozygous patients for the risk allele (GG) showed 2.17 (1.05-4.49) fold increased risk of MACE during hemodialysis than carriers of the protective allele (AA or AG). Diabetes mellitus was a strong enhancer of this effect.
  
  
  CONCLUSIONS
  Our results indicate that ANRIL polymorphisms may confer risk to development of MACE in incident patients on hemodialysis.
authors.affiliation: Biochemistry Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Arbiol-Roca
authors.affiliation: Biochemistry Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: A
authors.initials: A
authors.lastname: Padró-Miquel
authors.affiliation: Nephrology Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: M
authors.initials: M
authors.lastname: Hueso
authors.affiliation: Molecular Oncology Laboratory, IDIBELL, Barcelona, Spain.
authors.forename: E
authors.initials: E
authors.lastname: Navarro
authors.affiliation: Biochemistry Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: P
authors.initials: P
authors.lastname: Alía-Ramos
authors.affiliation: Nephrology Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: M T
authors.initials: MT
authors.lastname: González-Álvarez
authors.affiliation: Nephrology Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: I
authors.initials: I
authors.lastname: Rama
authors.affiliation: Nephrology Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: J
authors.initials: J
authors.lastname: Torras
authors.affiliation: Nephrology Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Grinyó
authors.affiliation: Nephrology Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain.
authors.forename: J M
authors.initials: JM
authors.lastname: Cruzado
authors.affiliation: Nephrology Department, IDIBELL, Hospital Universitari de Bellvitge, Barcelona, Spain. Electronic address: nlloberas@ub.edu.
authors.forename: N
authors.initials: N
authors.lastname: Lloberas
mesh_terms: D000368:Aged; D002318:Cardiovascular Diseases / Q000235:genetics*; D015331:Cohort Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006801:Humans; D015810:Linkage Disequilibrium; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D011446:Prospective Studies; D062085:RNA, Long Noncoding / Q000235:genetics*; D006435:Renal Dialysis; D051436:Renal Insufficiency, Chronic / Q000150:complications*
chemical_list: C561518:CDKN2B antisense RNA, human; D062085:RNA, Long Noncoding
keywords: ANRIL gene; Chronic kidney disease; Hemodialysis; Major adverse cardiovascular event; Single nucleotide polymorphism