title: FISH-in-CHIPS: A Microfluidic Platform for Molecular Typing of Cancer Cells.
abstract: Microfluidics offer powerful tools for the control, manipulation, and analysis of cells, in particular for the assessment of cell malignancy or the study of cell subpopulations. However, implementing complex biological protocols on chip remains a challenge. Sample preparation is often performed off chip using multiple manually performed steps, and protocols usually include different dehydration and drying steps that are not always compatible with a microfluidic format.Here, we report the implementation of a Fluorescence in situ Hybridization (FISH) protocol for the molecular typing of cancer cells in a simple and low-cost device. The geometry of the chip allows integrating the sample preparation steps to efficiently assess the genomic content of individual cells using a minute amount of sample. The FISH protocol can be fully automated, thus enabling its use in routine clinical practice.
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.|Univ Paris Diderot, Sorbonne Paris Cité, F-75205, Paris, France.|Université Paris Sorbonne Cité, INSERM UMR-S1147, Centre Universitaire des Saints-Pères, 45 rue des Saints-Pères, 75270, Paris, Cedex 06, France.
authors.forename: Karla
authors.initials: K
authors.lastname: Perez-Toralla
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Guillaume
authors.initials: G
authors.lastname: Mottet
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Ezgi
authors.initials: E
authors.lastname: Tulukcuoglu-Guneri
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.|University Paris-Diderot, PRES Paris Cité, INSERM/CNRS UMR944/7212, Paris, France.|Molecular Oncology Unit, APHP, Saint-Louis Hospital, Paris, France.
authors.forename: Jérôme
authors.initials: J
authors.lastname: Champ
authors.affiliation: Department of Medical Oncology, Institut Curie, 26 rue d'Ulm, 75005, Paris, France.
authors.forename: François-Clément
authors.initials: FC
authors.lastname: Bidard
authors.affiliation: Department of Medical Oncology, Institut Curie, 26 rue d'Ulm, 75005, Paris, France.|Université Paris Descartes, Paris, France.
authors.forename: Jean-Yves
authors.initials: JY
authors.lastname: Pierga
authors.affiliation: Department of Pathology, Institut Curie, Paris, France.
authors.forename: Jerzy
authors.initials: J
authors.lastname: Klijanienko
authors.affiliation: Telomeres & Cancer laboratory, Institut Curie, UPMC Univ. Paris 06, EquipeLabellisé « Ligue », Paris, France.
authors.forename: Irena
authors.initials: I
authors.lastname: Draskovic
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Malaquin
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Viovy
authors.affiliation: Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, 75005, Paris, France. stephanie.descroix@curie.fr.
authors.forename: Stéphanie
authors.initials: S
authors.lastname: Descroix
mesh_terms: D045744:Cell Line, Tumor; D004867:Equipment Design; D006801:Humans; D007091:Image Processing, Computer-Assisted / Q000379:methods; D017404:In Situ Hybridization, Fluorescence / Q000295:instrumentation / Q000379:methods*; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D058889:Molecular Typing / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics; D061848:Optical Imaging; D012984:Software
keywords: Cancer; FISH; Gene amplification; Microfluidic; Sample preparation