title: Methyl-β-cyclodextrin inhibits Ca2+-responses induced by glutoxim and molixan in macrophages.
abstract: Using Fura-2AM microfluorimetry, we have shown for the first time that methyl-β-cyclodextrin, inducing cholesterol extraction from membranes and raft disruption, significantly inhibits glutoxim- and molixan-induced Ca2+-responses in rat peritoneal macrophages. The results suggest that intact rafts are necessary for signaling cascade induced by glutoxim or molixan and leading to intracellular Ca2+concentration increase in macrophages.
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia. z.krutetskaya@spbu.ru.
authors.forename: Z I
authors.initials: ZI
authors.lastname: Krutetskaya
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: L S
authors.initials: LS
authors.lastname: Milenina
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Naumova
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: S N
authors.initials: SN
authors.lastname: Butov
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: V G
authors.initials: VG
authors.lastname: Antonov
authors.affiliation: St. Petersburg State University, Universitetskaya nab. 7/9, St. Petersburg, 199034, Russia.
authors.forename: A D
authors.initials: AD
authors.lastname: Nozdrachev
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism; D020013:Calcium Signaling / Q000187:drug effects / Q000502:physiology; D002413:Cations, Divalent / Q000378:metabolism; D002478:Cells, Cultured; D002784:Cholesterol / Q000378:metabolism; D004338:Drug Combinations; D004347:Drug Interactions; D005456:Fluorescent Dyes; D005470:Fluorometry; D016257:Fura-2 / Q000031:analogs & derivatives; D007288:Inosine / Q000494:pharmacology*; D057847:Lipid Regulating Agents / Q000494:pharmacology*; D017737:Macrophages, Peritoneal / Q000187:drug effects* / Q000378:metabolism; D021962:Membrane Microdomains / Q000187:drug effects / Q000378:metabolism; D049990:Membrane Transport Modulators / Q000494:pharmacology*; D009842:Oligopeptides / Q000494:pharmacology*; D017208:Rats, Wistar; D047392:beta-Cyclodextrins / Q000494:pharmacology*
chemical_list: D002413:Cations, Divalent; D004338:Drug Combinations; D005456:Fluorescent Dyes; D057847:Lipid Regulating Agents; D049990:Membrane Transport Modulators; D009842:Oligopeptides; D047392:beta-Cyclodextrins; C462699:glutoxim; C000611436:inosine glycyl-cysteinyl-glutamate disodium; C108732:methyl-beta-cyclodextrin; C049925:fura-2-am; D007288:Inosine; D002784:Cholesterol; D002118:Calcium; D016257:Fura-2