title: Probiotics enhance the effect of allergy immunotherapy on regulating antigen specific B cell activity in asthma patients.
abstract: Immune regulatory system dysfunction plays a role in the pathogenesis of asthma. The therapeutic effect of allergic asthma is to be improved. The immune regulatory function of probiotics has been recognized. This study tests a hypothesis thatClostridium butyricum(CB) enhances the effect of allergen specific immunotherapy (AIT) on asthma. In this study patients with allergic asthma were treated with AIT or/and CB for six months. The therapeutic effect and IgE production of the patients were observed. The results showed that administration with AIT alone alleviated the asthma symptoms; but the serum levels of interleukin (IL)-4, IL-5, IL-13 and specific IgE were not altered, which was markedly improved by the administration with CB plus AIT. Such effects were maintained only for two months in the patients treated with AIT alone; but maintained more than 12 months in those patients treated with both AIT and CB. CB facilitated AIT to induce IL-10+B cells (B10 cells) in asthma patients. AIT/CB therapy converted antigen specific B cells to antigen specific regulatory B cells. Butyrate modulated the gene transcription of IgE and IL-10 in the allergen specific B cells. In conclusion, administration of CB can enhance the therapeutic effect of AIT in the treatment of allergic asthma via facilitating generation of B10 cells.
authors.affiliation: Shenzhen Maternity & Child Health HospitalShenzhen 518052, China; The Center of Allergy & Immunology, School of Medicine, Shenzhen UniversityShenzhen 518060, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: The Center of Allergy & Immunology, School of Medicine, Shenzhen UniversityShenzhen 518060, China; Allergy Center, Otorhinolaryngology Hospital, the First Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510080, China.
authors.forename: Feng-Hong
authors.initials: FH
authors.lastname: Chen
authors.affiliation: The Center of Allergy & Immunology, School of Medicine, Shenzhen UniversityShenzhen 518060, China; Shenzhen ENT Institute and The Affiliated ENT Hospital of Shenzhen UniversityShenzhen 518116, China.
authors.forename: Shu-Qi
authors.initials: SQ
authors.lastname: Qiu
authors.affiliation: Shenzhen ENT Institute and The Affiliated ENT Hospital of Shenzhen UniversityShenzhen 518116, China; Brain Body Institute and Department of Pathology & Molecular Medicine, McMaster UniversityHamilton, ON L8N 4A6, Canada.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Yang
authors.affiliation: Shenzhen ENT Institute and The Affiliated ENT Hospital of Shenzhen UniversityShenzhen 518116, China; Department of Respirology, The First Hospital of Shanxi Medical UniversityTaiyuan 030001, China.
authors.forename: Huan-Ping
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: Shenzhen ENT Institute and The Affiliated ENT Hospital of Shenzhen UniversityShenzhen 518116, China; Brain Body Institute and Department of Pathology & Molecular Medicine, McMaster UniversityHamilton, ON L8N 4A6, Canada.
authors.forename: Jiang-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: Shenzhen ENT Institute and The Affiliated ENT Hospital of Shenzhen UniversityShenzhen 518116, China; Brain Body Institute and Department of Pathology & Molecular Medicine, McMaster UniversityHamilton, ON L8N 4A6, Canada.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Geng
authors.affiliation: Shenzhen ENT Institute and The Affiliated ENT Hospital of Shenzhen UniversityShenzhen 518116, China; Brain Body Institute and Department of Pathology & Molecular Medicine, McMaster UniversityHamilton, ON L8N 4A6, Canada.
authors.forename: Gui
authors.initials: G
authors.lastname: Yang
authors.affiliation: Shenzhen ENT Institute and The Affiliated ENT Hospital of Shenzhen UniversityShenzhen 518116, China; Brain Body Institute and Department of Pathology & Molecular Medicine, McMaster UniversityHamilton, ON L8N 4A6, Canada.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: Department of Allergy and Clinical Immunology, State Key Laboratory of Respiratory Disease, The First Affiliated Hospital, Guangzhou Medical University Guangzhou 510120, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: The Center of Allergy & Immunology, School of Medicine, Shenzhen University Shenzhen 518060, China.
authors.forename: Zhi-Gang
authors.initials: ZG
authors.lastname: Liu
authors.affiliation: Allergy Center, Otorhinolaryngology Hospital, the First Affiliated Hospital of Sun Yat-sen University Guangzhou 510080, China.
authors.forename: Hua-Bin
authors.initials: HB
authors.lastname: Li
authors.affiliation: The Center of Allergy & Immunology, School of Medicine, Shenzhen University Shenzhen 518060, China.
authors.forename: Ping-Chang
authors.initials: PC
authors.lastname: Yang
keywords: Allergy; IgE; allergen specific immunotherapy; asthma; probiotics