title: Evaluation of cancer cell deformability by microcavity array.
abstract: A cell entrapment device consisting of a microcavity array was used to analyze the deformability of MCF-10 human breast epithelial and MCF-7 human breast cancer cell lines by confocal laser scanning microscopy. Entrapment of up to 8 × 103cells was achieved within 3 min. Protrusions were formed at the bottom surface of the array with a pore size of 3 μm. Protrusion length increased at higher filtration pressures and could be used to distinguish between MCF-7 and MCF-10 cells. These results indicate that our system is useful for high-throughput deformability analysis of cancer cells, which can provide insight into the mechanisms underlying tumor cell malignancy.
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan. Electronic address: y-tomoko@cc.tuat.ac.jp.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Yoshino
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Tanaka
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Seita
authors.initials: S
authors.lastname: Nakamura
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Negishi
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Nozomi
authors.initials: N
authors.lastname: Shionoiri
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Masahito
authors.initials: M
authors.lastname: Hosokawa
authors.affiliation: Division of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Matsunaga
mesh_terms: D045744:Cell Line, Tumor; D002467:Cell Nucleus / Q000502:physiology; D048430:Cell Shape / Q000502:physiology*; D005452:Fluoresceins / Q000737:chemistry; D006801:Humans; D061986:MCF-7 Cells; D018613:Microscopy, Confocal; D009532:Nickel / Q000737:chemistry; D046888:Tissue Array Analysis
chemical_list: D005452:Fluoresceins; C069306:5-chloromethylfluorescein; D009532:Nickel
keywords: Breast cancer; Cell deformability