title: Personalised proteome analysis by means of protein microarrays made from individual patient samples.
abstract: DNA sequencing has advanced to a state that permits studying the genomes of individual patients as nearly a matter of routine. Towards analysing a tissue's protein content in a similar manner, we established a method for the production of microarrays that represent full-length proteins as they are encoded in individual specimens, exhibiting the particular variations, such as mutations or splice variations, present in these samples. From total RNA isolates, each transcript is copied to a specific location on the array by an on-chip polymerase elongation reaction, followed by in situ cell-free transcription and translation. These microarrays permit parallel analyses of variations in protein structure and interaction that are specific to particular samples.
authors.affiliation: Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.|Department of Chemistry, Faculty of Mathematics and Natural Sciences, Andalas University, Kampus Limau Manis, Padang, Indonesia.
authors.lastname: Syafrizayanti
authors.affiliation: Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
authors.forename: Smiths S
authors.initials: SS
authors.lastname: Lueong
authors.affiliation: Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.|Key Laboratory of Heavy Ion Radiation Medicine of Gansu Province, Institution of Modern Physics, Chinese Academy of Science, 509 Nanchang Road, 730000 Lanzhou, China.
authors.forename: Cuixia
authors.initials: C
authors.lastname: Di
authors.affiliation: Department of Biochemistry, University of Zürich, Winterthurerstraße 190, 8057 Zürich, Switzerland.
authors.forename: Jonas V
authors.initials: JV
authors.lastname: Schaefer
authors.affiliation: Department of Biochemistry, University of Zürich, Winterthurerstraße 190, 8057 Zürich, Switzerland.
authors.forename: Andreas
authors.initials: A
authors.lastname: Plückthun
authors.affiliation: Division of Functional Genome Analysis, Deutsches Krebsforschungszentrum (DKFZ), Im Neuenheimer Feld 580, 69120 Heidelberg, Germany.
authors.forename: Jörg D
authors.initials: JD
authors.lastname: Hoheisel
mesh_terms: D002474:Cell-Free System; D004247:DNA / Q000032:analysis; D003955:Diagnostic Tests, Routine; D005456:Fluorescent Dyes; D015870:Gene Expression; D016678:Genome; D006801:Humans; D020411:Oligonucleotide Array Sequence Analysis / Q000379:methods; D057285:Precision Medicine; D040081:Protein Array Analysis / Q000379:methods*; D020543:Proteome / Q000302:isolation & purification*; D012313:RNA / Q000302:isolation & purification
chemical_list: D005456:Fluorescent Dyes; D020543:Proteome; D012313:RNA; D004247:DNA