title: Light enhanced the accumulation of total fatty acids (TFA) and docosahexaenoic acid (DHA) in a newly isolated heterotrophic microalga Crypthecodinium sp. SUN.
abstract: In the present study, light illumination was found to be efficient in elevating the total fatty acid content in a newly isolated heterotrophic microalga, Crypthecodinium sp. SUN. Under light illumination, the highest total fatty acid and DHA contents were achieved at 96h as 24.9% of dry weight and 82.8mgg-1dry weight, respectively, which were equivalent to 1.46-fold and 1.68-fold of those under the dark conditions. The elevation of total fatty acid content was mainly contributed by an increase of neutral lipids at the expense of starches. Moreover, light was found to alter the cell metabolism and led to a higher specific growth rate, higher glucose consumption rate and lower non-motile cell percentage. This is the first report that light can promote the total fatty acids accumulation in Crypthecodinium without growth inhibition.
authors.affiliation: Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Dongzhe
authors.initials: D
authors.lastname: Sun
authors.affiliation: Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Mao
authors.affiliation: Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wu
authors.affiliation: Runke Bioengineering Co. Ltd., Zhangzhou, Fujian, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Liu
authors.affiliation: Institute for Food & Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China; BIC-ESAT, College of Engineering, Peking University, Beijing 100871, China. Electronic address: sfchencoe@pku.edu.cn.
authors.forename: Feng
authors.initials: F
authors.lastname: Chen
mesh_terms: D004281:Docosahexaenoic Acids / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism*; D052836:Heterotrophic Processes / Q000502:physiology*; D008027:Light; D058086:Microalgae / Q000254:growth & development / Q000378:metabolism*
chemical_list: D005227:Fatty Acids; D004281:Docosahexaenoic Acids
keywords: Crypthecodinium sp.; DHA; Neutral lipids; Starch; Total fatty acids