title: Cryopreservation of Induced Pluripotent Stem Cells.
abstract: Induced pluripotent stem (iPS) cells have attracted attention as a promising cell source for medical treatment that could replace marrow stromal cells (MSCs) and adipose tissue-derived stem cells (ASCs). These pluripotent cells can be induced in vitro and in vivo to differentiate into various tissues and organs. The cells will be useful for regenerative medicine, cell therapy, and drug screening. Vitrification is used, as well as a rapid-freeze method, for colony-forming iPS cells. However, the method requires a high degree of technical skill. We herein report a more convenient method for freezing iPS cells in suspension. We examined the proliferation potency of cryopreserved mouse iPS cells using culture medium, 10% DMSO, 10% glycerol, 5% DMSO, 5% glycerol, 5% DMSO + 5% glycerol, cell-freezing medium-DMSO, cell-freezing medium-glycerol, Cell Banker 1, Cell Banker 1+, Cell Banker 2, and Cell Banker 3 as cryopreservation solutions. Among them, Cell Banker 3 showed the highest efficacy in terms of the proliferation of mouse iPS cells. The mouse iPS cells cryopreserved in Cell Banker 3 at -80°C for 12 months maintained a high proliferation rate and an undifferentiated status. The formation of teratomas was also examined. In conclusion, Cell Banker 3 allows for freezing of iPS cells in suspension.
authors.affiliation: Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine, Higashi-ku, Nagoya, Japan; †Department of Oral Disease Research, National Center for Geriatrics and Gerontology, Aichi, Japan; ‡Clinical Research Center, National Center for Child Health and Development, Tokyo, Japan.
authors.forename: Yoshitaka
authors.initials: Y
authors.lastname: Miyamoto
authors.affiliation: § Department of Gastroenterological Surgery, Transplant and Surgical Oncology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences , Okayama , Japan.
authors.forename: Hirofumi
authors.initials: H
authors.lastname: Noguchi
authors.affiliation: Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine , Higashi-ku, Nagoya , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Yukawa
authors.affiliation: Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine , Higashi-ku, Nagoya , Japan.
authors.forename: Koichi
authors.initials: K
authors.lastname: Oishi
authors.affiliation: † Department of Oral Disease Research, National Center for Geriatrics and Gerontology , Aichi , Japan.
authors.forename: Kenji
authors.initials: K
authors.lastname: Matsushita
authors.affiliation: ¶ Department of Biomedical Sciences, Chubu University College of Life and Health Sciences , Aichi , Japan.
authors.forename: Hisashi
authors.initials: H
authors.lastname: Iwata
authors.affiliation: Department of Advanced Medicine in Biotechnology and Robotics, Nagoya University Graduate School of Medicine , Higashi-ku, Nagoya , Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Hayashi
keywords: Cryopreservation; Induced pluripotent stem (iPS) cells; Pluripotency; Slow freezing