title: [Effects of methylseleninic acid on cisplatin-resistant ovarian cancer cells (SKOV3/DDP) and the mechanisms].
abstract: To investigate the reversal effect of methylseleninic acid on cisplatin (DDP)-resistant ovarian cancer cells and the underlying mechanisms.  Methods: SKOV3/DDP cells were incubated with cisplatin at different concentrations for 48 h, then the proliferation rate of SKOV3/DDP cells was detected by MTT assays, and the expression of β-catenin in SKOV3/DDP cells was examined by Western blot. The inhibitory effect of methyl-seleninic acid (MSA) combined with DDP at different concentrations on SKOV3/DDP cells was assayed by MTT method. Western blot was used to detect the expression of β-catenin protein in the cells.  Results: The inhibitory rate for proliferation in DDP-treated SKOV3/DDP cells with different concentrations is lower than that in the SKOV3 cells (P<0.05); β-catenin expression in SKOV3/DDP cells was significantly higher than that in the SKOV3 cells (P<0.05). The inhibitory rate for proliferation in SKOV3/DDP cells with different concentrations of MSA was increased with the increase in concentration (P<0.05). The inhibitory rate for proliferation in SKOV3/DDP cells with 2 or 6 μmol/L MSA plus cisplatin was lower than that in cisplatin alone group (P<0.05). β-catenin expression in SKOV3 /DDP cells with 2 or 6 μmol/L MSA plus cisplatin was higher than that in the cisplatin alone group (P<0.05).  Conclusion: MSA can reverse cisplatin resistance on SKOV3 / DDP cells, which may be related to the decrease in β-catenin expression.
authors.affiliation: Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Feng
authors.affiliation: Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Min
authors.initials: M
authors.lastname: Xue
authors.affiliation: Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Ning
authors.initials: N
authors.lastname: Jiang
authors.affiliation: Department of Obstetrics and Gynecology, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Xinliang
authors.initials: X
authors.lastname: Deng
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002277:Carcinoma / Q000503:physiopathology; D045744:Cell Line, Tumor / Q000502:physiology; D049109:Cell Proliferation / Q000187:drug effects; D002945:Cisplatin / Q000494:pharmacology; D019008:Drug Resistance, Neoplasm / Q000187:drug effects* / Q000235:genetics; D005260:Female; D006801:Humans; D016566:Organoselenium Compounds / Q000494:pharmacology*; D010051:Ovarian Neoplasms / Q000503:physiopathology; D051176:beta Catenin / Q000187:drug effects / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; D016566:Organoselenium Compounds; D051176:beta Catenin; C008493:methylselenic acid; D002945:Cisplatin