title: Assessment of ocular discomfort caused by 5 shampoos using the Slug Mucosal Irritation test.
abstract: Assessment of ocular discomfort caused by veterinary care products is less legitimately regulated than that caused by human care products. The Slug Mucosal Irritation (SMI) assay was adapted to evaluate canine hygiene shampoos to predict ocular discomfort. Experiments were performed using four commercial canine shampoos, a baby care product, and two controls (ArtTear® and BAC1%). Groups of 3 slugs were tested with 5% dilution of the 7 test substances. The negative control (ArtTear®) was the best tolerated. The baby care product Mixa bébé as well as Douxo Entretien Démêlant and Phlox Shampooing Entretien were classified to cause mild ocular discomfort. Together with the positive control (BAC 1%), Shampooing Physiologique Virbac and Physiovet Shampooing were considered to cause severe ocular discomfort. Different intensities of ocular discomfort were measured for veterinary care products. The SMI model was considered as a reproducible and adaptable evaluation method for screening veterinary care products causing ocular discomfort.
authors.affiliation: Institut de Recherche Clinique Animale, Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, 7 avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France. Electronic address: jeanyanique.petit@hotmail.fr.
authors.forename: Jean-Yanique
authors.initials: JY
authors.lastname: Petit
authors.affiliation: Institut de Recherche Clinique Animale, Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, 7 avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Doré
authors.affiliation: Institut de Recherche Clinique Animale, Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, 7 avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France.
authors.forename: Geneviève
authors.initials: G
authors.lastname: Marignac
authors.affiliation: Institut de Recherche Clinique Animale, Université Paris-Est, Ecole Nationale Vétérinaire d'Alfort, 7 avenue du Général de Gaulle, 94704 Maisons-Alfort Cedex, France.
authors.forename: Sébastien
authors.initials: S
authors.lastname: Perrot
mesh_terms: D000818:Animals; D005123:Eye / Q000187:drug effects*; D049851:Gastropoda; D006203:Hair Preparations / Q000633:toxicity*; D007509:Irritants / Q000633:toxicity*; D009092:Mucous Membrane / Q000187:drug effects*; D018675:Toxicity Tests / Q000379:methods
chemical_list: D006203:Hair Preparations; D007509:Irritants
keywords: Ocular discomfort; Slug Mucosal Irritation test; Veterinary shampoos