title: A genetic method to enhance the accumulation of S-adenosylmethionine in yeast.
abstract: S-Adenosylmethionine (SAM) is a key component of sulphur amino acid metabolism in living organisms and is synthesised from methionine and adenosine triphosphate by methionine adenosyltransferase. This molecule serves as the main biological methyl donor due to its active methylthio ether group. Notably, SAM has shown beneficial effects in clinical trials for the treatment of alcoholic liver disease, depression and joint pain. Due to the high potential value of SAM, current research efforts are attempting to develop a more rapid, cost-effective and higher yielding SAM production method than the conventional production system. In this mini-review, we describe the previously reported yeast gene that contributes to SAM accumulation by overexpression, mutation or deletion and summarise the genetic approach for the production of SAM in large industrial quantities.
authors.affiliation: National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-0046, Japan. kanai@nrib.go.jp.
authors.forename: Muneyoshi
authors.identifier: http://orcid.org/0000-0001-8673-8273
authors.initials: M
authors.lastname: Kanai
authors.affiliation: Hiroshima Research Center for Healthy Aging, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8530, Japan.
authors.forename: Masaki
authors.initials: M
authors.lastname: Mizunuma
authors.affiliation: National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-0046, Japan.|Graduate School of Biosphere Science, Hiroshima University, 1-4-4, Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8528, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Fujii
authors.affiliation: National Research Institute of Brewing, 3-7-1 Kagamiyama, Higashi-hiroshima, Hiroshima, 739-0046, Japan.|Graduate School of Biosphere Science, Hiroshima University, 1-4-4, Kagamiyama, Higashi-hiroshima, Hiroshima, 739-8528, Japan.|Present Address: Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime, 790-8566, Japan.
authors.forename: Haruyuki
authors.initials: H
authors.lastname: Iefuji
mesh_terms: D008715:Methionine / Q000378:metabolism; D010843:Pichia / Q000378:metabolism; D012436:S-Adenosylmethionine / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000378:metabolism*
chemical_list: D012436:S-Adenosylmethionine; D008715:Methionine
keywords: Methionine synthesis pathway; Pichia pastoris; S-Adenosylmethionine; Saccharomyces cerevisiae; Sake yeast; Yeast