title: Characterisation of Early-Life Fecal Microbiota in Susceptible and Healthy Pigs to Post-Weaning Diarrhoea.
abstract: Early-life microbial exposure is of particular importance to growth, immune system development and long-lasting health. Hence, early microbiota composition is a promising predictive biomarker for health and disease but still remains poorly characterized in regards to susceptibility to diarrhoea. In the present study, we aimed to assess if gut bacterial community diversity and composition during the suckling period were associated with differences in susceptibility of pigs to post-weaning diarrhoea. Twenty piglets from 5 sows (4 piglets / litter) were weaned in poor housing conditions to challenge their susceptibility to post-weaning diarrhoea. Two weeks after weaning, 13 pigs exhibited liquid faeces during 2 or 3 days and were defined as diarrhoeic (D) pigs. The other 7 pigs did not have diarrhea during the whole post-weaning experimental periodand were defined as healthy (H) pigs. Using a molecular characterisation of fecal microbiota with CE-SSCP fingerprint, Next Generation Sequencing and qPCR, we show that D and H pigs were mainly discriminated as early as postnatal day (PND) 7, i.e. 4 weeks before post-weaning diarrhoea occurence. At PND 7 H pigs displayed a lower evenness and a higher abundance of Prevotellaceae, Lachnospiraceae, Ruminocacaceae and Lactobacillaceae compared to D pigs. The sPLS regression method indicates that these bacterial families were strongly correlated to a higher Bacteroidetes abundance observed in PND 30 H pigs one week before diarrhoea. These results emphasize the potential of early microbiota diversity and composition as being an indicator of susceptibility to post-weaning diarrhoea. Furthermore, they support the health promoting strategies of pig herds through gut microbiota engineering.
authors.affiliation: UP 2012.10.101.EGEAL, Institut Polytechnique LaSalle Beauvais, rue Pierre Waguet Beauvais, France.
authors.forename: Samir
authors.initials: S
authors.lastname: Dou
authors.affiliation: UP 2012.10.101.EGEAL, Institut Polytechnique LaSalle Beauvais, rue Pierre Waguet Beauvais, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Gadonna-Widehem
authors.affiliation: INRA, UR1341 ADNC, Domaine de la Prise, Saint-Gilles, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Rome
authors.affiliation: UP 2012.10.101.EGEAL, Institut Polytechnique LaSalle Beauvais, rue Pierre Waguet Beauvais, France.
authors.forename: Dounia
authors.initials: D
authors.lastname: Hamoudi
authors.affiliation: UP 2012.10.101.EGEAL, Institut Polytechnique LaSalle Beauvais, rue Pierre Waguet Beauvais, France.
authors.forename: Larbi
authors.initials: L
authors.lastname: Rhazi
authors.affiliation: Institut polytechnique LaSalle Beauvais, Direction de la recherche, rue Pierre Waguet Beauvais, France.
authors.forename: Lyes
authors.initials: L
authors.lastname: Lakhal
authors.affiliation: INRA, UMR 703 APEX, Ecole Nationale Vétérinaire Agroalimentaire et de l'Alimentation Nantes-Atlantique (Oniris), Nantes, France.
authors.forename: Thibaut
authors.initials: T
authors.lastname: Larcher
authors.affiliation: UP 2012.10.101.EGEAL, Institut Polytechnique LaSalle Beauvais, rue Pierre Waguet Beauvais, France.
authors.forename: Narges
authors.initials: N
authors.lastname: Bahi-Jaber
authors.affiliation: INVIVO-NSA, Direction Scientifique Technique et Innovation, Département Innovation et Prospectives, Talhouët Saint Nolff, France.
authors.forename: Arturo
authors.initials: A
authors.lastname: Pinon-Quintana
authors.affiliation: INVIVO-NSA, Direction Scientifique Technique et Innovation, Département Innovation et Prospectives, Talhouët Saint Nolff, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Guyonvarch
authors.affiliation: INRA, UR1341 ADNC, Domaine de la Prise, Saint-Gilles, France.
authors.forename: Isabelle L E
authors.initials: IL
authors.lastname: Huërou-Luron
authors.affiliation: UP 2012.10.101.EGEAL, Institut Polytechnique LaSalle Beauvais, rue Pierre Waguet Beauvais, France.
authors.forename: Latifa
authors.initials: L
authors.lastname: Abdennebi-Najar
mesh_terms: D000818:Animals; D016022:Case-Control Studies; D003967:Diarrhea / Q000382:microbiology / Q000662:veterinary*; D000069196:Gastrointestinal Microbiome; D007777:Lactobacillaceae / Q000235:genetics / Q000302:isolation & purification; D018720:Prevotella / Q000235:genetics / Q000302:isolation & purification; D045857:Ruminococcus / Q000235:genetics / Q000302:isolation & purification; D013552:Swine; D013553:Swine Diseases / Q000382:microbiology*; D014886:Weaning