title: Metabolic profiling of root exudates from two ecotypes of Sedum alfredii treated with Pb based on GC-MS.
abstract: Phytoremediation is an effective method to remediate Pb-contaminated soils and root exudates play an important role in this process. Based on gas chromatography-mass spectrometry (GC-MS) and metabolomics method, this study focuses on the comparative metabolic profiling analysis of root exudates from the Pb-accumulating and non-accumulating ecotypes of Sedum alfredii treated with 0 and 50 μmol/L Pb. The results obtained show that plant type and Pb stress can significantly change the concentrations and species of root exudates, and fifteen compounds were identified and assumed to be potential biomarkers. Leaching experiments showed that l-alanine, l-proline and oxalic acid have a good effect to activate Pb in soil, glyceric acid and 2-hydroxyacetic acid have a general effect to activate Pb in soil. 4-Methylphenol and 2-methoxyphenol might be able to activate Pb in soil, glycerol and diethyleneglycol might be able to stabilize Pb in soil, but these activation effect and stabilization effect were all not obvious.
authors.affiliation: Key Laboratory of Regional Environment and Eco-Remediation of Ministry of Educatione, College of Environment, Shenyang University, Shenyang 110044, China.
authors.forename: Qing
authors.initials: Q
authors.lastname: Luo
authors.affiliation: Key Laboratory of Regional Environment and Eco-Remediation of Ministry of Educatione, College of Environment, Shenyang University, Shenyang 110044, China.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Regional Environment and Eco-Remediation of Ministry of Educatione, College of Environment, Shenyang University, Shenyang 110044, China.
authors.forename: Li-Na
authors.initials: LN
authors.lastname: Sun
authors.affiliation: Key Laboratory of Regional Environment and Eco-Remediation of Ministry of Educatione, College of Environment, Shenyang University, Shenyang 110044, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
mesh_terms: D000409:Alanine / Q000032:analysis; D015415:Biomarkers / Q000032:analysis / Q000378:metabolism; D060146:Ecotype; D008401:Gas Chromatography-Mass Spectrometry; D007854:Lead / Q000633:toxicity*; D055442:Metabolome / Q000187:drug effects; D019815:Oxalic Acid / Q000032:analysis; D018517:Plant Roots / Q000187:drug effects / Q000378:metabolism; D025341:Principal Component Analysis; D011392:Proline / Q000032:analysis; D031963:Sedum / Q000187:drug effects* / Q000254:growth & development / Q000378:metabolism; D012989:Soil Pollutants / Q000737:chemistry / Q000633:toxicity
chemical_list: D015415:Biomarkers; D012989:Soil Pollutants; D007854:Lead; D011392:Proline; D019815:Oxalic Acid; D000409:Alanine