title: Overexpression of PTP4A3 in ETV6-RUNX1 acute lymphoblastic leukemia.
abstract: Cell signalling, which is often derailed in cancer, is a network of multiple interconnected pathways with numerous feedback mechanisms. Dynamics of cell signalling is intimately regulated by addition and removal of phosphate groups by kinases and phosphatases. We examined expression of members of the PTP4A family of phosphatases across acute leukemias. While expression of PTP4A1 and PTP4A2 remained relatively unchanged across diseases, PTP4A3 showed marked overexpression in ETV6-RUNX1 and BCR-ABL1 subtypes of precursor B cell acute lymphoblastic leukemia. We show that PTP4A3 is regulated by the ETV6-RUNX1 fusion, but noticed no marked impact on cell viability either after PTP4A3 silencing or treatment with a PTP4A3 inhibitor. Regulation of PTP4A3 expression is altered in specific subgroups of acute leukemias and this is likely brought about by expression of the aberrant fusion genes.
authors.affiliation: Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland. Electronic address: toni.gronroos@staff.uta.fi.
authors.forename: Toni
authors.initials: T
authors.lastname: Grönroos
authors.affiliation: Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors.forename: Susanna
authors.initials: S
authors.lastname: Teppo
authors.affiliation: Institute of Biomedicine, School of Medicine, University of Eastern Finland, Kuopio, Finland.
authors.forename: Juha
authors.initials: J
authors.lastname: Mehtonen
authors.affiliation: Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors.forename: Saara
authors.initials: S
authors.lastname: Laukkanen
authors.affiliation: Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland; Institute of Biosciences and Medical Technology, University of Tampere, Finland.
authors.forename: Thomas
authors.initials: T
authors.lastname: Liuksiala
authors.affiliation: Institute of Biosciences and Medical Technology, University of Tampere, Finland; Department of Signal Processing, Tampere University of Technology, Tampere, Finland.
authors.forename: Matti
authors.initials: M
authors.lastname: Nykter
authors.affiliation: Institute of Biomedicine, School of Medicine, University of Eastern Finland, Kuopio, Finland.
authors.forename: Merja
authors.initials: M
authors.lastname: Heinäniemi
authors.affiliation: Tampere Center for Child Health Research, University of Tampere and Tampere University Hospital, Tampere, Finland.
authors.forename: Olli
authors.initials: O
authors.lastname: Lohi
mesh_terms: D002460:Cell Line; D002470:Cell Survival; D050676:Core Binding Factor Alpha 2 Subunit; D016044:Fusion Proteins, bcr-abl; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009363:Neoplasm Proteins / Q000235:genetics*; D015514:Oncogene Proteins, Fusion; D054198:Precursor Cell Lymphoblastic Leukemia-Lymphoma / Q000235:genetics*; D017027:Protein Tyrosine Phosphatases / Q000235:genetics*; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D050676:Core Binding Factor Alpha 2 Subunit; D016044:Fusion Proteins, bcr-abl; D009363:Neoplasm Proteins; D015514:Oncogene Proteins, Fusion; D017027:Protein Tyrosine Phosphatases; C102421:TEL-AML1 fusion protein; D000097764:ETS Translocation Variant 6 Protein; C484730:PTP4A3 protein, human
keywords: ETV6-RUNX1; Leukemia; PTP4A3