title: Behavior of Oxidative Stress Markers in Alcoholic Liver Cirrhosis Patients.
abstract: Alcohol is the most socially accepted addictive substance worldwide, and its metabolism is related with oxidative stress generation. The aim of this work was to evaluate the role of oxidative stress in alcoholic liver cirrhosis (ALC). This study included 187 patients divided into two groups: ALC, classified according to Child-Pugh score, and a control group. We determined the levels of reduced and oxidized glutathione (GSH and GSSG) and the GSH/GSSG ratio by an enzymatic method in blood. Also, protein carbonyl and malondialdehyde (MDA) content were estimated in serum. MDA levels increased in proportion to the severity of damage, whereas the GSH and GSSG levels decreased and increased, respectively, at different stages of cirrhosis. There were no differences in the GSH/GSSG ratio and carbonylated protein content between groups. We also evaluated whether the active consumption of or abstinence from alcoholic beverages affected the behavior of these oxidative markers and only found differences in the MDA, GSH, and GSSG determination and the GSH/GSSG ratio. Our results suggest that alcoholic cirrhotic subjects have an increase in oxidative stress in the early stages of disease severity and that abstinence from alcohol consumption favors the major antioxidant endogen: GSH in patients with advanced disease severity.
authors.affiliation: HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico; Departamento de Farmacología, Facultad de Medicina Mexicali, UABC, Mexicali, BC, Mexico.
authors.forename: Marina
authors.initials: M
authors.lastname: Galicia-Moreno
authors.affiliation: HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico.
authors.forename: Dorothy
authors.initials: D
authors.lastname: Rosique-Oramas
authors.affiliation: HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico.
authors.forename: Zaira
authors.initials: Z
authors.lastname: Medina-Avila
authors.affiliation: HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico.
authors.forename: Tania
authors.initials: T
authors.lastname: Álvarez-Torres
authors.affiliation: HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico.
authors.forename: Dalia
authors.initials: D
authors.lastname: Falcón
authors.affiliation: Liver Clinic, Gastroenterology Service, Hospital General de México, Mexico City, Mexico.
authors.forename: Fátima
authors.initials: F
authors.lastname: Higuera-de la Tijera
authors.affiliation: Blood Bank Service, Hospital General de México, Mexico City, Mexico.
authors.forename: Yadira L
authors.initials: YL
authors.lastname: Béjar
authors.affiliation: Liver Unit and Molecular Medicine, University Hospital, Universidad Autónoma de Nuevo León, Monterrey, NL, Mexico.
authors.forename: Paula
authors.initials: P
authors.lastname: Cordero-Pérez
authors.affiliation: Liver Unit and Molecular Medicine, University Hospital, Universidad Autónoma de Nuevo León, Monterrey, NL, Mexico.
authors.forename: Linda
authors.initials: L
authors.lastname: Muñoz-Espinosa
authors.affiliation: Liver Clinic, Gastroenterology Service, Hospital General de México, Mexico City, Mexico.
authors.forename: José Luis
authors.initials: JL
authors.lastname: Pérez-Hernández
authors.affiliation: HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico; Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, Mexico.
authors.forename: David
authors.initials: D
authors.lastname: Kershenobich
authors.affiliation: HIPAM Lab, Experimental Medicine Unit, School of Medicine, Universidad Nacional Autónoma de México, Hospital General de México, Mexico City, Mexico.
authors.forename: Gabriela
authors.identifier: 0000-0003-1961-9885
authors.initials: G
authors.lastname: Gutierrez-Reyes
mesh_terms: D000328:Adult; D000410:Alanine Transaminase / Q000097:blood; D001219:Aspartate Aminotransferases / Q000097:blood; D015415:Biomarkers / Q000097:blood*; D016022:Case-Control Studies; D005260:Female; D005978:Glutathione / Q000097:blood; D019803:Glutathione Disulfide / Q000097:blood; D006801:Humans; D008099:Liver / Q000378:metabolism; D008104:Liver Cirrhosis, Alcoholic / Q000378:metabolism / Q000473:pathology*; D008297:Male; D008315:Malondialdehyde / Q000097:blood; D008875:Middle Aged; D018384:Oxidative Stress; D050050:Protein Carbonylation; D012720:Severity of Illness Index
chemical_list: D015415:Biomarkers; D008315:Malondialdehyde; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005978:Glutathione; D019803:Glutathione Disulfide