title: Specific membrane dynamics during neural stem cell division.
abstract: Neural stem and progenitor cells in the developing cerebral cortex, but also when grown in culture, display a range of distinct phenomena during cytokinesis. Cleavage furrow ingression in neural progenitor cells can bisect their basal processes and, later on, result in midbody formation at the apical surface. After abscission, these midbodies are released as membrane-bound particles into the extracellular space, in contrast to uptake and degradation of postabscission midbodies in other cell types. Whether these cellular dynamics are unique to neural stem cells, or more ubiquitously found, and what biological significance these processes have for cell differentiation or cell-cell communication, are open questions that require a combination of approaches. Here, we discuss techniques to study the specific membrane dynamics underlying the basal process splitting and postabscission midbody release in neural stem cells. We provide some basic concepts and protocols to isolate, enrich and stain released midbodies, and follow midbody dynamics over time. Moreover, we discuss techniques to prepare cortical sections for high-voltage electron microscopy to visualize the fine basal processes of progenitor cells.
authors.affiliation: Institute of Epigenetics and Stem Cells, Munich, Germany.
authors.forename: A
authors.initials: A
authors.lastname: Ettinger
authors.affiliation: Korea Brain Research Institute, Daegu, Korea.
authors.forename: Y
authors.initials: Y
authors.lastname: Kosodo
authors.affiliation: Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.
authors.forename: W B
authors.initials: WB
authors.lastname: Huttner
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000235:genetics; D002455:Cell Division / Q000235:genetics; D002462:Cell Membrane; D002469:Cell Separation / Q000379:methods*; D058948:Cell Tracking / Q000379:methods*; D002540:Cerebral Cortex / Q000648:ultrastructure; D048749:Cytokinesis / Q000235:genetics*; D006367:HeLa Cells; D006801:Humans; D008856:Microscopy, Fluorescence; D058953:Neural Stem Cells / Q000648:ultrastructure*
keywords: Basal process; Differential centrifugation; High-voltage electron microscopy; Live-cell microscopy; Membrane dynamics; Midbody