title: Brucella abortus is Prevalent in Both Humans and Animals in Bangladesh.
abstract: To determine the role of different Brucella (B.) spp. in Bangladesh, 62 animal samples and 500 human sera were tested. Animal samples from cattle, goats and sheep (including milk, bull semen, vaginal swabs and placentas) were cultured for Brucella spp. Three test-positive human sera and all animal samples were screened by Brucella genus-specific real-time PCR (RT-PCR), and positive samples were then tested by IS711 RT-PCR to detect B. abortus and B. melitensis DNA. Only B. abortus DNA was amplified from 13 human and six animal samples. This is the first report describing B. abortus as the aetiological agent of brucellosis in occupationally exposed humans in Bangladesh. Of note is failure to detect B. melitensis DNA, the species most often associated with human brucellosis worldwide. Further studies are required to explore the occurrence of Brucella melitensis in Bangladesh.
authors.affiliation: Department of Medicine, Bangladesh Agricultural University, Mymensingh, Bangladesh.|Research Unit of Epidemiology and Risk Analysis applied to the Veterinary Sciences (UREAR-Ulg), Fundamental and Applied Research for Animals & Health (FARAH), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.|Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerpen, Belgium.
authors.forename: A K M A
authors.identifier: 0000-0001-9660-4949
authors.initials: AKMA
authors.lastname: Rahman
authors.affiliation: Research Unit of Epidemiology and Risk Analysis applied to the Veterinary Sciences (UREAR-Ulg), Fundamental and Applied Research for Animals & Health (FARAH), Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, University of Liège, Liège, Belgium.
authors.forename: C
authors.initials: C
authors.lastname: Saegerman
authors.affiliation: Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerpen, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Berkvens
authors.affiliation: Federal Research Institute for Animal Health, Reference Laboratory for Brucellosis and CEM, Jena, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Melzer
authors.affiliation: Federal Research Institute for Animal Health, Reference Laboratory for Brucellosis and CEM, Jena, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Neubauer
authors.affiliation: Department of Bacteriology and Immunology, Veterinary and Agrochemical Research Center, Brussels, Belgium.
authors.forename: D
authors.initials: D
authors.lastname: Fretin
authors.affiliation: Department of Applied Mathematics, Computer Science and Statistics, Faculty of Sciences, Ghent University, Ghent, Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Abatih
authors.affiliation: School of Veterinary Science, The University of Sydney, Camden, NSW, Australia.
authors.forename: N
authors.initials: N
authors.lastname: Dhand
authors.affiliation: School of Veterinary Science, The University of Sydney, Camden, NSW, Australia.
authors.forename: M P
authors.initials: MP
authors.lastname: Ward
mesh_terms: D000818:Animals; D001459:Bangladesh / Q000453:epidemiology; D002003:Brucella abortus; D002006:Brucellosis / Q000453:epidemiology / Q000662:veterinary*; D004269:DNA, Bacterial / Q000235:genetics*; D006801:Humans
chemical_list: D004269:DNA, Bacterial
keywords: 
  Brucella abortus
  ; Bangladesh; animals; clinical samples; humans; real-time PCR