title: Loss of Nrf2 exacerbates the visual deficits and optic neuritis elicited by experimental autoimmune encephalomyelitis.
abstract: Optic neuritis, inflammation of the optic nerve, is experienced by most patients with multiple sclerosis (MS) and is typically characterized by episodes of acute, monocular vision loss. These episodes of inflammation can lead to damage or degeneration of the retinal ganglion cells (RGCs), the axons of which comprise the optic nerve. Experimental autoimmune encephalomyelitis (EAE) is a well-established model of MS in which mice are immunized to produce a neuroautoimmunity that recapitulates the cardinal hallmarks of human disease, namely, inflammation, demyelination, and neurodegeneration of the brain, spinal cord, and optic nerve. Inflammation-associated oxidative stress plays a key role in promoting spinal cord damage in EAE. However, the role of oxidative stress in optic neuritis and the associated visual deficits has not been studied. To address this gap in research, we sought to determine how a deficiency in the master antioxidant transcription factor (using nuclear factor-E2-related factor [Nrf2]-deficient mice) affects visual pathology in the EAE model.
  
  
  
  EAE was induced in 8-week-old wild-type (WT) and Nrf2 knockout (KO) mice by immunization against the myelin oligodendrocyte glycoprotein (MOG) peptide antigen. Motor deficits were monitored daily, as was visual acuity using the established functional optokinetic tracking (OKT) assay. Mice were euthanized 21 days post-immunization for histological analyses. The optic nerves were paraffin-embedded and stained with hematoxylin and eosin (H&E) or immune cell type-specific antibodies to analyze inflammatory infiltrates. The retinas were flatmounted and stained with an RGC-specific antibody, and the RGCs were counted to assess neurodegeneration. T-helper (Th) cell-associated cytokines were measured in spleens with enzyme-linked immunosorbent assay (ELISA). Immune analyses of healthy, non-EAE mice were characterized with flow cytometry to assess the baseline immune cell profiles.
  
  
  
  Female Nrf2 KO mice exhibited more severe EAE-induced motor deficits compared with female WT mice. In both genders, EAE elicited more severe visual acuity deficits, inflammation of the optic nerve, and RGC degeneration in KO mice compared with their strain- and age-matched WT counterparts. Visual acuity deficits were primarily present in (and only exacerbated in) one eye of each mouse. Excess inflammatory cells within the optic nerves of the KO mice were primarily comprised of T-cells, and greater RGC degeneration in the KO mice was most prevalent in the central retina compared with the peripheral retina. Nrf2 KO spleens exhibited an increased Th1- but not Th17-associated immune response. This enhanced pathology in the KO mice was not due to global differences in immune system development between the two genotypes.
  
  
  
  This is the first study to report that genetic ablation of Nrf2 exacerbates visual deficits, inflammation of the optic nerve, and RGC degeneration in a murine model of MS, suggesting that Nrf2 plays a neuro- and cytoprotective role in EAE-associated optic neuritis.
authors.affiliation: Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, OK; Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK.
authors.forename: Chelsea M
authors.initials: CM
authors.lastname: Larabee
authors.affiliation: Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK.
authors.forename: Shruti
authors.initials: S
authors.lastname: Desai
authors.affiliation: Arthritis and Clinical Immunology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK.
authors.forename: Agnieshka
authors.initials: A
authors.lastname: Agasing
authors.affiliation: Arthritis and Clinical Immunology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK.
authors.forename: Constantin
authors.initials: C
authors.lastname: Georgescu
authors.affiliation: Arthritis and Clinical Immunology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK.
authors.forename: Jonathan D
authors.initials: JD
authors.lastname: Wren
authors.affiliation: Arthritis and Clinical Immunology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK.
authors.forename: Robert C
authors.initials: RC
authors.lastname: Axtell
authors.affiliation: Oklahoma Center for Neuroscience, University of Oklahoma Health Sciences Center, Oklahoma City, OK; Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK.
authors.forename: Scott M
authors.initials: SM
authors.lastname: Plafker
mesh_terms: D000818:Animals; D016207:Cytokines / Q000378:metabolism; D004681:Encephalomyelitis, Autoimmune, Experimental / Q000150:complications* / Q000473:pathology* / Q000503:physiopathology; D005260:Female; D007249:Inflammation / Q000473:pathology; D008297:Male; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D051267:NF-E2-Related Factor 2 / Q000172:deficiency* / Q000378:metabolism; D009900:Optic Nerve / Q000473:pathology; D009902:Optic Neuritis / Q000150:complications* / Q000473:pathology* / Q000503:physiopathology; D010243:Paralysis / Q000473:pathology; D012165:Retinal Ganglion Cells / Q000378:metabolism / Q000473:pathology; D012727:Sex Characteristics; D013154:Spleen / Q000473:pathology; D018417:Th1 Cells / Q000378:metabolism; D014792:Visual Acuity
chemical_list: D016207:Cytokines; D051267:NF-E2-Related Factor 2; C495636:Nfe2l2 protein, mouse