title: The liver transcriptome of suckermouth armoured catfish (Pterygoplichthys anisitsi, Loricariidae): Identification of expansions in defensome gene families.
abstract: Pterygoplichthys is a genus of related suckermouth armoured catfishes native to South America, which have invaded tropical and subtropical regions worldwide. Physiological features, including an augmented resistance to organic xenobiotics, may have aided their settlement in foreign habitats. The liver transcriptome of Pterygoplichthys anisitsi was sequenced and used to characterize the diversity of mRNAs potentially involved in the responses to natural and anthropogenic chemicals. In total, 66,642 transcripts were assembled. Among the identified defensome genes, cytochromes P450 (CYP) were the most abundant, followed by sulfotransferases (SULT), nuclear receptors (NR) and ATP binding cassette transporters (ABC). A novel expansion in the CYP2Y subfamily was identified, as well as an independent expansion of the CYP2AAs. Two expansions were also observed among SULT1. Thirty-two transcripts were classified into twelve subfamilies of NR, while 21 encoded ABC transporters. The diversity of defensome transcripts sequenced herein could contribute to this species' resistance to organic xenobiotics.
authors.affiliation: Laboratório de Toxicologia Ambiental, Escola Nacional de Saúde Pública (ENSP), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, Brasil; Laboratório de Genética Molecular de Microrganismos, Instituto Oswaldo Cruz (IOC), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, Brasil; Woods Hole Oceanographic Institution (WHOI), Woods Hole, MA 02543, USA. Electronic address: parente@ensp.fiocruz.br.
authors.forename: Thiago E
authors.initials: TE
authors.lastname: Parente
authors.affiliation: Laboratório de Toxicologia Ambiental, Escola Nacional de Saúde Pública (ENSP), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, Brasil.
authors.forename: Daniel A
authors.initials: DA
authors.lastname: Moreira
authors.affiliation: Laboratório de Toxicologia Ambiental, Escola Nacional de Saúde Pública (ENSP), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, Brasil.
authors.forename: Maithê G P
authors.initials: MGP
authors.lastname: Magalhães
authors.affiliation: Laboratório de Toxicologia Ambiental, Escola Nacional de Saúde Pública (ENSP), Fundação Oswaldo Cruz (FIOCRUZ), Rio de Janeiro 21040-900, Brasil.
authors.forename: Paula C C
authors.initials: PCC
authors.lastname: de Andrade
authors.affiliation: Unidade de Genômica, Instituto Nacional do Cancer (INCA), Rio de Janeiro 20230-130, Brasil.
authors.forename: Carolina
authors.initials: C
authors.lastname: Furtado
authors.affiliation: Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, MA 02142, USA.
authors.forename: Brian J
authors.initials: BJ
authors.lastname: Haas
authors.affiliation: Woods Hole Oceanographic Institution (WHOI), Woods Hole, MA 02543, USA.
authors.forename: John J
authors.initials: JJ
authors.lastname: Stegeman
authors.affiliation: Woods Hole Oceanographic Institution (WHOI), Woods Hole, MA 02543, USA.
authors.forename: Mark E
authors.initials: ME
authors.lastname: Hahn
mesh_terms: D000818:Animals; D002397:Catfishes / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D005810:Multigene Family; D013020:South America; D059467:Transcriptome
keywords: ABC transporters; Nuclear receptors; P450; RNA-Seq; SULT