title: The Functional Haplotypes ofCHRM3Modulate mRNA Expression and Associate with Bladder Cancer among a Chinese Han Population in Kaohsiung City.
abstract: Bladder cancer is one of the major cancer types and both environmental factors and genetic background play important roles in its pathology. Kaohsiung is a high industrialized city in Taiwan, and here we focused on this region to evaluate the genetic effects on bladder cancer. Muscarinic acetylcholine receptor M3 (CHRM3) was reported as a key receptor in different cancer types.CHRM3is located at 1q42-43 which was reported to associate with bladder cancer. Our study attempted to delineate whether genetic variants ofCHRM3contribute to bladder cancer in Chinese Han population in south Taiwan. Five selected SNPs (rs2165870, rs10802789, rs685550, rs7520974, and rs3738435) were genotyped for 30 bladder cancer patients and 60 control individuals and genetic association studies were performed. Five haplotypes (GTTAT, ATTGT, GCTAC, ACTAC, and ACCAC) were found significantly associated with lowCHRM3mRNA level and contributed to increased susceptibility of bladder cancer in Kaohsiung city after rigid 10000 consecutive permutation tests. To our knowledge, this is the first genetic association study that reveals the genetic contribution ofCHRM3gene in bladder cancer etiology.
authors.affiliation: Division of Urology, Department of Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
authors.forename: Chiang-Ting
authors.initials: CT
authors.lastname: Wang
authors.affiliation: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan.
authors.forename: Tsung-Ming
authors.initials: TM
authors.lastname: Chen
authors.affiliation: Division of Urology, Department of Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
authors.forename: Chien-Tai
authors.initials: CT
authors.lastname: Mei
authors.affiliation: Division of Urology, Department of Medicine, Kaohsiung Armed Forces General Hospital, Kaohsiung, Taiwan.
authors.forename: Chun-Feng
authors.initials: CF
authors.lastname: Chang
authors.affiliation: Department of Oceanography, National Sun Yat-sen University, Kaohsiung 804, Taiwan.
authors.forename: Li-Lian
authors.initials: LL
authors.lastname: Liu
authors.affiliation: Department and Graduate Institute of Aquaculture, National Kaohsiung Marine University, Kaohsiung, Taiwan.
authors.forename: Kuo-Hsun
authors.initials: KH
authors.lastname: Chiu
authors.affiliation: Department of Aquaculture, National Pingtung University of Science and Technology, Pingtung, Taiwan.
authors.forename: Tsung-Meng
authors.initials: TM
authors.lastname: Wu
authors.affiliation: Department of Health Risk Management, China Medical University, Taichung, Taiwan.
authors.forename: Yu-Ching
authors.initials: YC
authors.lastname: Lan
authors.affiliation: Asia-Pacific Biotech Developing Inc., Kaohsiung, Taiwan.
authors.forename: Wen-Sheng
authors.initials: WS
authors.lastname: Liu
authors.affiliation: Graduate Institute of Biomedical Science, China Medical University, Taichung, Taiwan; Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan; Cancer Biology and Drug Discovery Ph.D. Program, College of Medicine, China Medical University, Taichung, Taiwan.
authors.forename: Ya-Huey
authors.identifier: 0000-0002-5757-8634
authors.initials: YH
authors.lastname: Chen
authors.affiliation: Institute of Biomedical Sciences, National Chung Hsing University, Taichung, Taiwan.
authors.forename: Yi-Mei Joy
authors.identifier: 0000-0002-8509-0628
authors.initials: YJ
authors.lastname: Lin
mesh_terms: D000368:Aged; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D056726:Genetic Association Studies; D020022:Genetic Predisposition to Disease; D005838:Genotype; D006239:Haplotypes; D006801:Humans; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide; D012333:RNA, Messenger / Q000235:genetics; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic / Q000235:genetics*; D013624:Taiwan; D001749:Urinary Bladder Neoplasms / Q000235:genetics* / Q000473:pathology
chemical_list: C000595896:CHRM3 protein, human; D012333:RNA, Messenger; D043587:Receptor, Muscarinic M3; D011976:Receptors, Muscarinic