title: Burkholderia contaminansBiofilm Regulating Operon and Its Distribution in Bacterial Genomes.
abstract: Biofilm formation byBurkholderiaspp. is a principal cause of lung chronic infections in cystic fibrosis patients. A "lacking biofilm production" (LBP) strainB. contaminansGIMC4587:Bct370-19 has been obtained by insertion modification of clinical strain with plasposon mutagenesis. It has an interrupted transcriptional response regulator (RR) gene. The focus of our investigation was a two-component signal transduction system determination, including this RR.B. contaminansclinical and LBP strains were analyzed by whole genome sequencing and bioinformatics resources. A four-component operon (BiofilmReg) has a key role in biofilm formation. The relative location (i.e., by being separated by another gene) of RR and histidine kinase genes is unique in BiofilmReg. Orthologs were found in other members of the Burkholderiales order. Phylogenetic analysis of strains containing BiofilmReg operons demonstrated evidence for earlier inheritance of a three-component operon. During further evolution one lineage acquired a fourth gene, whereas others lost the third component of the operon. Mutations in sensor domains have created biodiversity which is advantageous for adaptation to various ecological niches. Different speciesBurkholderiaandAchromobacterstrains all demonstrated similar BiofilmReg operon structure. Therefore, there may be an opportunity to develop a common drug which is effective for treating all these causative agents.
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, Moscow 123098, Russia.
authors.forename: Olga L
authors.identifier: 0000-0001-7206-3594
authors.initials: OL
authors.lastname: Voronina
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, Moscow 123098, Russia.
authors.forename: Marina S
authors.identifier: 0000-0003-1945-0397
authors.initials: MS
authors.lastname: Kunda
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, Moscow 123098, Russia.
authors.forename: Natalia N
authors.identifier: 0000-0001-5361-870X
authors.initials: NN
authors.lastname: Ryzhova
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, Moscow 123098, Russia.
authors.forename: Ekaterina I
authors.identifier: 0000-0002-0437-9404
authors.initials: EI
authors.lastname: Aksenova
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, Moscow 123098, Russia.
authors.forename: Andrey N
authors.initials: AN
authors.lastname: Semenov
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, Moscow 123098, Russia; I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.
authors.forename: Yulia M
authors.identifier: 0000-0002-8547-1711
authors.initials: YM
authors.lastname: Romanova
authors.affiliation: N.F. Gamaleya Federal Research Center for Epidemiology and Microbiology, Ministry of Health of Russia, Gamaleya Street 18, Moscow 123098, Russia; I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.
authors.forename: Alexandr L
authors.initials: AL
authors.lastname: Gintsburg
mesh_terms: D042402:Achromobacter; D018441:Biofilms; D019121:Burkholderia Infections / Q000382:microbiology; D042602:Burkholderia cepacia complex / Q000235:genetics* / Q000378:metabolism*; D019295:Computational Biology; D003550:Cystic Fibrosis / Q000150:complications / Q000382:microbiology; D004275:DNA, Ribosomal / Q000235:genetics; D015964:Gene Expression Regulation, Bacterial; D018014:Gene Transfer Techniques; D016678:Genome; D016680:Genome, Bacterial; D000071677:Histidine Kinase / Q000235:genetics; D006801:Humans; D008171:Lung Diseases / Q000382:microbiology*; D016296:Mutagenesis; D009876:Operon; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D015398:Signal Transduction; D014158:Transcription, Genetic
chemical_list: D004275:DNA, Ribosomal; D000071677:Histidine Kinase