title: Programmed death-1 ligands 1 and 2 expression in cutaneous squamous cell carcinoma and their relationship with tumour- infiltrating dendritic cells.
abstract: The programmed death-1 (PD-1) receptor ligands, PD-L1 and PD-L2, are co-stimulatory molecules that contribute to the negative regulation of T lymphocyte activation. It is still unclear whether there is correlation between PD-L1 or PD-L2 and tumour-infiltrating dendritic cells (TIDCs) in cutaneous squamous cell carcinoma (CSCC). The aim of this study was to analyse PD-L1 and PD-L2 expression and dendritic cells infiltration in tumour tissue of CSCC patients and investigate their clinical significance. Immunohistochemical analysis was used to evaluate the expression of PD-L1, PD-L2, CD1a and CD83 in 61 CSCC tissues. The immunofluoresence double-labelling technique was performed to detect the co-expression of PD-L1 or PD-L2 and CD1a or CD83 in tumour tissues. We found that 25 of 61 cases CSCC (40·98%) exhibited positivity for PD-L1, whereas 37 of 61 cases CSCC (60·66%) exhibited positivity for PD-L2. A higher percentage of CD1a-positive cases were observed on both PD-L1-positive and PD-L2-positive specimens compared with that of CD83-positive cases (92·29% versus 37·60%, 83·20% versus 33·16%). The expression of PD-L1 and PD-L2 on CD1a+cells was significantly higher than that on CD83+cells in tumour tissues of CSCC patients. Furthermore, the expression rate of PD-L1 was associated with UICC stage, and the expression rate of PD-L2 was associated with predominant differentiation and tumour size in CSCC. Our results indicated that higher expression of PD-L1 and PD-L2 on CD1a+cells than that on CD83+cells in CSCC tumour tissues may contribute to negative regulation in anti-tumour immune responses.
authors.affiliation: Department of Dermatology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Jiao
authors.affiliation: Department of Dermatology, First Affiliated Hospital, Soochow University, Suzhou, China.|Department of Clinical Immunology Laboratory, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: C
authors.initials: C
authors.lastname: Liu
authors.affiliation: Biomedical Research Center, University of Rostock, Rostock, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Dermatology, Soochow University Affiliated Children's Hospital, Suzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Li
authors.affiliation: Department of Dermatology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: F
authors.initials: F
authors.lastname: Fang
authors.affiliation: Department of Dermatology, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Qian
authors.affiliation: Department of Dermatology, First Affiliated Hospital, Soochow University, Suzhou, China.|Department of Clinical Immunology Laboratory, First Affiliated Hospital, Soochow University, Suzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Zhang
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015703:Antigens, CD / Q000378:metabolism; D060890:B7-H1 Antigen / Q000378:metabolism*; D002294:Carcinoma, Squamous Cell / Q000276:immunology* / Q000473:pathology; D003713:Dendritic Cells / Q000276:immunology*; D005260:Female; D005858:Germany; D006801:Humans; D007150:Immunohistochemistry; D008213:Lymphocyte Activation / Q000276:immunology; D016246:Lymphocytes, Tumor-Infiltrating / Q000276:immunology*; D008297:Male; D008875:Middle Aged; D061027:Programmed Cell Death 1 Ligand 2 Protein / Q000378:metabolism*; D012878:Skin Neoplasms / Q000276:immunology* / Q000473:pathology
chemical_list: D015703:Antigens, CD; D060890:B7-H1 Antigen; D061027:Programmed Cell Death 1 Ligand 2 Protein
keywords: cutaneous squamous cell carcinoma; programmed death-1 ligand 1; programmed death-1 ligand 2; tumour-infiltrating dendritic cells