title: Isolation and Identification of Intestinal Myeloid Cells.
abstract: The identification of conventional dendritic cells (cDCs) and macrophages (mϕ) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from each other and from other intestinal myeloid cells. Here we detail the protocols we have developed to isolate live leukocytes from steady state mouse small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs, monocytes and macrophages in such preparations.
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK.|Inflammation Research Centre (IRC), Laboratory of Immunoregulation, VIB Ghent University, Ghent (Zwijnaarde), 9052, Belgium.
authors.forename: Charlotte L
authors.initials: CL
authors.lastname: Scott
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK.|MRC Centre for Inflammation Research, Queens Medical Research Institute, University of Edinburgh, Edinburgh, EH16 4TJ, Scotland, UK.
authors.forename: Calum C
authors.initials: CC
authors.lastname: Bain
authors.affiliation: Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK. Allan.Mowat@glasgow.ac.uk.
authors.forename: Allan McI
authors.initials: AM
authors.lastname: Mowat
mesh_terms: D000818:Animals; D000906:Antibodies / Q000737:chemistry; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D015415:Biomarkers / Q000378:metabolism; D002469:Cell Separation / Q000295:instrumentation / Q000379:methods*; D017364:Collagenases / Q000737:chemistry; D003106:Colon / Q000166:cytology* / Q000276:immunology; D003713:Dendritic Cells / Q000166:cytology / Q000276:immunology; D003851:Deoxyribonucleases / Q000737:chemistry; D010450:Endopeptidases / Q000737:chemistry; D005434:Flow Cytometry / Q000379:methods*; D015870:Gene Expression; D007421:Intestine, Small / Q000166:cytology* / Q000276:immunology; D008264:Macrophages / Q000166:cytology / Q000276:immunology; D051379:Mice; D009000:Monocytes / Q000166:cytology / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods*
chemical_list: D000906:Antibodies; D015703:Antigens, CD; D015415:Biomarkers; D003851:Deoxyribonucleases; D010450:Endopeptidases; D017364:Collagenases; C023139:dispase
keywords: Dendritic cells; Identification; Intestine; Isolation; Macrophages; Subsets