title: CD95-Mediated Proton Regulation.
abstract: The Na+/H+exchanger NHE1 is at the crossroads of a large diversity of signaling pathways, whose activation modifies the cooperative response of the transporter to intracellular H+ions. Here we show how the activation of the Na+/H+exchanger NHE1 by the cleaved ligand of CD95 can be measured. We demonstrate two different methods designed to set intracellular pH at precise values. Then we show how these can be coupled to fast kinetics of lithium transport, which will enable to measure the NHE1 activity like for an enzyme, because they will yield rates of transport.
authors.affiliation: LP2M UMR 7370 Faculté de Médecine, Université Nice-Sophia Antipolis, 28 Avenue de Valombrose, 06107, Nice, France.|Laboratories of Excellence, Ion Channels Sciences and Therapeutics, Université Lille, Villeneuve d'Ascq, France.
authors.forename: Auréa
authors.initials: A
authors.lastname: Cophignon
authors.affiliation: LP2M UMR 7370 Faculté de Médecine, Université Nice-Sophia Antipolis, 28 Avenue de Valombrose, 06107, Nice, France.|Laboratories of Excellence, Ion Channels Sciences and Therapeutics, Université Lille, Villeneuve d'Ascq, France.
authors.forename: Mallorie
authors.initials: M
authors.lastname: Poët
authors.affiliation: LP2M UMR 7370 Faculté de Médecine, Université Nice-Sophia Antipolis, 28 Avenue de Valombrose, 06107, Nice, France.|Laboratories of Excellence, Ion Channels Sciences and Therapeutics, Université Lille, Villeneuve d'Ascq, France.|LPCM EA4667, Université de Picardie Jules Verne, 33 rue st Leu, 80000, Amiens, France.
authors.forename: Michael
authors.initials: M
authors.lastname: Monet
authors.affiliation: LP2M UMR 7370 Faculté de Médecine, Université Nice-Sophia Antipolis, 28 Avenue de Valombrose, 06107, Nice, France.|Laboratories of Excellence, Ion Channels Sciences and Therapeutics, Université Lille, Villeneuve d'Ascq, France.
authors.forename: Michel
authors.initials: M
authors.lastname: Tauc
authors.affiliation: LP2M UMR 7370 Faculté de Médecine, Université Nice-Sophia Antipolis, 28 Avenue de Valombrose, 06107, Nice, France. Laurent.Counillon@unice.fr.|Laboratories of Excellence, Ion Channels Sciences and Therapeutics, Université Lille, Villeneuve d'Ascq, France. Laurent.Counillon@unice.fr.
authors.forename: Laurent
authors.initials: L
authors.lastname: Counillon
mesh_terms: D002478:Cells, Cultured; D006863:Hydrogen-Ion Concentration; D042541:Intracellular Space / Q000378:metabolism; D017136:Ion Transport; D007700:Kinetics; D011522:Protons; D000074082:Sodium-Hydrogen Exchanger 1 / Q000378:metabolism; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D011522:Protons; D000074082:Sodium-Hydrogen Exchanger 1; D019014:fas Receptor
keywords: CD95 signaling; Intracellular acidification; Ion transport; Kinetic measurements