title: Selective Tuning of Elastin-like Polypeptide Properties via Methionine Oxidation.
abstract: We have designed and prepared a recombinant elastin-like polypeptide (ELP) containing precisely positioned methionine residues, and performed the selective and complete oxidation of its methionine thioether groups to both sulfoxide and sulfone derivatives. Since these oxidation reactions substantially increase methionine residue polarity, they were found to be a useful means to precisely adjust the temperature responsive behavior of ELPs in aqueous solutions. In particular, lower critical solution temperatures were found to be elevated in oxidized sample solutions, but were not eliminated. These transition temperatures were found to be further tunable by the use of solvents containing different Hofmeister salts. Overall, the ability to selectively and fully oxidize methionine residues in ELPs proved to be a convenient postmodification strategy for tuning their transition temperatures in aqueous media.
authors.affiliation: Université de Bordeaux/Bordeaux INP, ENSCBP , and CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Rosine
authors.initials: R
authors.lastname: Petitdemange
authors.affiliation: Université de Bordeaux/Bordeaux INP, ENSCBP , and CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Elisabeth
authors.initials: E
authors.lastname: Garanger
authors.affiliation: Université de Bordeaux/Bordeaux INP, ENSCBP , and CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Laure
authors.identifier: 0000-0003-2351-8796
authors.initials: L
authors.lastname: Bataille
authors.affiliation: Université de Bordeaux/Bordeaux INP and CNRS, Chimie et Biologie des Membranes et des Nano-objets (UMR5248), Allée Geoffroy Saint Hilaire, Pessac 33600, France.
authors.forename: Wilfrid
authors.initials: W
authors.lastname: Dieryck
authors.affiliation: Université de Bordeaux/Bordeaux INP and CNRS, Chimie et Biologie des Membranes et des Nano-objets (UMR5248), Allée Geoffroy Saint Hilaire, Pessac 33600, France.
authors.forename: Katell
authors.initials: K
authors.lastname: Bathany
authors.affiliation: Université de Bordeaux/Bordeaux INP, ENSCBP , and CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Bertrand
authors.initials: B
authors.lastname: Garbay
authors.affiliation: Department of Chemistry and Biochemistry, and Department of Bioengineering, University of California , Los Angeles, California 90095, United States.
authors.forename: Timothy J
authors.identifier: 0000-0002-0594-5025
authors.initials: TJ
authors.lastname: Deming
authors.affiliation: Université de Bordeaux/Bordeaux INP, ENSCBP , and CNRS, Laboratoire de Chimie des Polymères Organiques (UMR5629), 16 avenue Pey-Berland, Pessac 33607, France.
authors.forename: Sébastien
authors.identifier: 0000-0003-0465-8603
authors.initials: S
authors.lastname: Lecommandoux
mesh_terms: D000595:Amino Acid Sequence; D001483:Base Sequence; D004549:Elastin / Q000737:chemistry*; D006801:Humans; D008715:Methionine / Q000737:chemistry*; D010084:Oxidation-Reduction; D010455:Peptides / Q000737:chemistry*; D044366:Transition Temperature; D014867:Water / Q000737:chemistry*
chemical_list: D010455:Peptides; D014867:Water; D004549:Elastin; D008715:Methionine