title: Iridoids and anti-inflammatory properties of n-butanol extract of Linaria tingitana Boiss. & Reut.
abstract: The analysis of the n-butanol extract of Linaria tingitana (BELT) by HPLC-DAD-ESI/MS allowed the identification of four iridoids structurally confirmed by NMR and ESI-MS/MS extensive experiments data. The subjection of BELT to the anti-inflammatory activity showed that it exhibited a concentration dependent stabilisation of HRBC membrane, inhibition of protein denaturation and nitric oxide scavenging effect in the in vitro process. These results were confirmed in the in vivo experiments which showed that BELT was found to be most pronounced at 200 mg/kg after carrageenan injection which significantly reduced the swelling in both early and late phases of carrageenan-evoked oedema, as well as a significant reduce at the accumulation of infiltrating cells, inhibition of the myeloperoxidase activity and suppressed the lipid peroxidation. These results were supported by the histological analysis which revealed the reduction of oedema and cells infiltration, this might be influenced by the synergistic action of the above isolated compounds.
authors.affiliation: a Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie , Université des Frères Mentouri Constantine , Constantine , Algeria.
authors.forename: Mourad
authors.initials: M
authors.lastname: Hanfer
authors.affiliation: b Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine , Constantine , Algeria.|c Laboratorio di Chimica Bioorganica, Dipartimento di Fisica , Università di Trento , Povo- Trento , Italy.
authors.forename: Thamere
authors.initials: T
authors.lastname: Cheriet
authors.affiliation: a Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie , Université des Frères Mentouri Constantine , Constantine , Algeria.
authors.forename: Souad
authors.initials: S
authors.lastname: Ameddah
authors.affiliation: c Laboratorio di Chimica Bioorganica, Dipartimento di Fisica , Università di Trento , Povo- Trento , Italy.
authors.forename: Ines
authors.initials: I
authors.lastname: Mancini
authors.affiliation: b Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine , Constantine , Algeria.
authors.forename: Ramdane
authors.initials: R
authors.lastname: Seghiri
authors.affiliation: a Laboratoire de Biologie et Environnement, Faculté des Sciences de la Nature et de la Vie , Université des Frères Mentouri Constantine , Constantine , Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Menad
authors.affiliation: b Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine , Constantine , Algeria.
authors.forename: Samir
authors.initials: S
authors.lastname: Benayache
authors.affiliation: b Unité de Valorisation des Ressources Naturelles, Molécules Bioactives et Analyse Physicochimiques et Biologiques (VARENBIOMOL) , Université des Frères Mentouri Constantine , Constantine , Algeria.
authors.forename: Fadila
authors.initials: F
authors.lastname: Benayache
mesh_terms: D020001:1-Butanol / Q000737:chemistry; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology*; D002351:Carrageenan / Q000633:toxicity; D002851:Chromatography, High Pressure Liquid; D004353:Drug Evaluation, Preclinical / Q000379:methods; D004487:Edema / Q000139:chemically induced / Q000188:drug therapy; D006801:Humans; D039823:Iridoids / Q000032:analysis / Q000737:chemistry* / Q000494:pharmacology*; D032221:Linaria / Q000737:chemistry*; D008297:Male; D009569:Nitric Oxide / Q000378:metabolism; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D011489:Protein Denaturation / Q000187:drug effects; D017208:Rats, Wistar; D021241:Spectrometry, Mass, Electrospray Ionization; D053719:Tandem Mass Spectrometry
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D039823:Iridoids; D010936:Plant Extracts; D009569:Nitric Oxide; D020001:1-Butanol; D002351:Carrageenan
keywords: Anti-inflammatory activity; Iridoids; LC-MS analysis; Linaria tingitana