title: 1H-NMR-based metabolomic studies of bisphenol A in zebrafish (Danio rerio).
abstract: Proton nuclear magnetic resonance (1H-NMR) spectroscopy was used to study the response of zebrafish (Danio rerio) to increasing concentrations of bisphenol A (4,4'-(propane-2,2-diyl)diphenol, BPA). Orthogonal partial least squares discriminant analysis (OPLS-DA) was applied to detect aberrant metabolomic profiles after 72 h of BPA exposure at all levels tested (0.01, 0.1, and 1.0 mg/L). The OPLS-DA score plots showed that BPA exposure caused significant alterations in the metabolome. The metabolomic changes in response to BPA exposure generally exhibited nonlinear patterns, with the exception of reduced levels of several metabolites, including glutamine, inosine, lactate, and succinate. As the level of BPA exposure increased, individual metabolite patterns indicated that the zebrafish metabolome was subjected to severe oxidative stress. Interestingly, ATP levels increased significantly at all levels of BPA exposure. In the present study, we demonstrated the applicability of1H-NMR-based metabolomics to identify the discrete nature of metabolic changes.
authors.affiliation: a Department of Chemistry , Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University , Busan , Korea.
authors.forename: Changshin
authors.initials: C
authors.lastname: Yoon
authors.affiliation: a Department of Chemistry , Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University , Busan , Korea.
authors.forename: Dahye
authors.initials: D
authors.lastname: Yoon
authors.affiliation: a Department of Chemistry , Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University , Busan , Korea.
authors.forename: Junghee
authors.initials: J
authors.lastname: Cho
authors.affiliation: a Department of Chemistry , Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University , Busan , Korea.
authors.forename: Siwon
authors.initials: S
authors.lastname: Kim
authors.affiliation: a Department of Chemistry , Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University , Busan , Korea.
authors.forename: Heonho
authors.initials: H
authors.lastname: Lee
authors.affiliation: a Department of Chemistry , Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University , Busan , Korea.
authors.forename: Hyeonsoo
authors.initials: H
authors.lastname: Choi
authors.affiliation: a Department of Chemistry , Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University , Busan , Korea.
authors.forename: Suhkmann
authors.initials: S
authors.lastname: Kim
mesh_terms: D000818:Animals; D001559:Benzhydryl Compounds / Q000633:toxicity*; D016018:Least-Squares Analysis; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome / Q000187:drug effects; D055432:Metabolomics / Q000379:methods*; D018384:Oxidative Stress / Q000187:drug effects; D010636:Phenols / Q000633:toxicity*; D018675:Toxicity Tests / Q000379:methods; D015027:Zebrafish / Q000378:metabolism*; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: 1H-NMR spectroscopy; Bisphenol A; metabolites; zebrafish (Danio rerio)