title: Rotaxane probes for protease detection by129Xe hyperCEST NMR.
abstract: We report a CB6 rotaxane for the129Xe hyperCEST NMR detection of matrix metalloprotease 2 (MMP-2) activity. MMP-2 is overexpressed in cancer tissue, and hence is a cancer marker. A peptide containing an MMP-2 recognition sequence was incorporated into the rotaxane, synthesized via CB6-promoted click chemistry. Upon cleavage of the rotaxane by MMP-2, CB6 became accessible for129Xe@CB6 interactions, leading to protease-responsive hyperCEST activation.
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu and Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA 94720, USA.
authors.forename: Clancy C
authors.identifier: http://orcid.org/0000-0001-6663-9112
authors.initials: CC
authors.lastname: Slack
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu.
authors.forename: Joel A
authors.identifier: http://orcid.org/0000-0003-0411-1755
authors.initials: JA
authors.lastname: Finbloom
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu and Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA 94720, USA.
authors.forename: Keunhong
authors.initials: K
authors.lastname: Jeong
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu.
authors.forename: Carson J
authors.initials: CJ
authors.lastname: Bruns
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu and Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA.
authors.forename: David E
authors.initials: DE
authors.lastname: Wemmer
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu and Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA 94720, USA.
authors.forename: Alexander
authors.initials: A
authors.lastname: Pines
authors.affiliation: Department of Chemistry, University of California, Berkeley, Berkeley, CA 94720, USA. mbfrancis@berkeley.edu and Materials Sciences Division, Lawrence Berkeley National Laboratories, Berkeley, CA 94720, USA.
authors.forename: Matthew B
authors.identifier: http://orcid.org/0000-0003-2837-2538
authors.initials: MB
authors.lastname: Francis