title: Anticancer and Cytotoxic Activities of [Cu(C6H16N2O2)2][Ni(CN)4] and [Cu(C6H16N2O2)Pd(CN)4] Cyanidometallate Compounds on HT29, HeLa, C6 and Vero Cell Lines.
abstract: BACKGROUND
  In cancer, apoptosis relevant proteins-such as CaM kinase, Bcl-2 or P53, topoisomerase I, cell migration feature and DNA/BSA-macromolecules represent significant targets for current chemotherapeutics.
  
  
  OBJECTIVE
  We recently reported two coordination compounds-[Cu(C6H16N2O2)2][Ni(CN)4] (1) and [Cu(C6H16 N2O2)Pd(CN)4] (2)-together with their IR spectra, magnetic properties, thermal analyses and crystal structures. Herein, we describe the ability of these complexes to induce apoptosis in relevant proteins and stimulate topoisomerase I activity, cell migration velocity and DNA/BSA binding properties.
  
  
  METHODS
  The in vitro antiproliferative effects and cell toxicity of both compounds were investigated through pharmacological measurement techniques, and interactions between both compounds and CT-DNA/BSA were studied with UV-Vis spectroscopy and fluorescence spectroscopy. Results &amp; Conclusion: Studies on cells revealed that 2 (i) demonstrated a high antiproliferative effect, which was higher toward HeLa and C6 cancer cells than toward healthy Vero cells; (ii) impaired the migration of HeLa cells; (iii) altered the P53-Bcl-2 ratio in favor of apoptosis; (iv) strongly bound to DNA/BSA macromolecules; and (v) inhibited human topoisomerase I and KpnI or BamHI restriction endonucleases. In conclusion, this preliminary information demonstrates that 2 may represent a promising antiproliferative agent and a potential candidate for a therapeutic approach against HeLa.
authors.affiliation: Department of Molecular Biology and Genetics, Faculty of Science, Gaziosmanpaşa University, P.O. Box: 60240, Tokat, Turkey.
authors.forename: Ali
authors.initials: A
authors.lastname: Aydın
authors.affiliation: Department of Bioengineering, Faculty of Engineering, Tunceli University, Tunceli, Turkey.
authors.forename: Sengul Aslan
authors.initials: SA
authors.lastname: Korkmaz
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Tunceli University, Tunceli, Turkey.
authors.forename: Veysel
authors.initials: V
authors.lastname: Demir
authors.affiliation: Department of Molecular Biology and Genetics, Gaziosmanpaşa University, Tokat, Turkey.
authors.forename: Saban
authors.initials: S
authors.lastname: Tekin
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D002460:Cell Line; D049109:Cell Proliferation / Q000187:drug effects; D002522:Chlorocebus aethiops; D004354:Drug Screening Assays, Antitumor; D006801:Humans; D013057:Spectrum Analysis / Q000379:methods; D014709:Vero Cells
chemical_list: D000970:Antineoplastic Agents
keywords: Cyanidometallate compound; antiproliferative effect; apoptosis; copper; nickel; palladium