title: Expressions of Inhibitors of DNA Binding-1 and Matrix Metalloproteinase-9 in Colorectal Adenocarcinoma Tissues and Their Correlations with Microvessel Density.
abstract: Objective To explore the expressions of inhibitors of DNA binding-1 (Id-1) and matrix metalloproteinase-9 (MMP-9) in colorectal carcinoma tissues and its correlation with microvessel density (MVD). Methods The expressions of Id-1 and MMP-9 as well as CD34-labelled MVD in colorectal adenocarcinoma tissues (n=50) and normal adjacent tissues (n=50) were examined by immunohistochemistry. Results The positive expressions of Id-1 and MMP-9 were seen in 72.00% (36/50) and 78.00%(39/50) of colorectal adenocarcinoma tissues,which were significantly higher than those [24.00%(12/50) and 28.00% (14/50)] in normal adjacent tissues (P=0.000). The MVD value (17.22±2.08) in colorectal adenocarcinoma tissues was significantly higher than that (5.36±2.17) in normal adjacent tissues (P=0.000). The expressions of Id-1 and MMP-9 and MVD were significantly correlated with serosa invasion,TNM stage,carcinoembryonic antigen(+),lymph node metastasis,vascular invasion,and liver metastasis (all P<0.05) but not with the patient's age,gender,tumor size,and differentiation degree (all P>0.05). The MVD value with Id-1 and MMP-9 positive expression were significantly higher than those with Id-1 and MMP-9 negative expression (all P=0.000). The expression of Id-1 in colorectal adenocarcinoma tissues showed significantly positive correlation with that of MMP-9 (r=0.429,P=0.000). Cox multivariate analysis showed that Id-1 and MMP-9 expressions were independent prognostic factors for colorectal carcinoma. Conclusions The high expressions of Id-1 and MMP-9 have high correlations with the development and progression of colorectal adenocarcinoma and have positive correlation with MVD. Both of them may be involved in the microvascular generation and the invasion and hematogenous metastasis of colorectal carcinoma.
authors.affiliation: Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Xue-Liang
authors.initials: XL
authors.lastname: Wu
authors.affiliation: Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Jun
authors.initials: J
authors.lastname: Xue
authors.affiliation: Department of Ultrasound,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Li-Kun
authors.initials: LK
authors.lastname: Wang
authors.affiliation: Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Dong-Dong
authors.initials: DD
authors.lastname: Yang
authors.affiliation: Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Ming
authors.initials: M
authors.lastname: Qu
authors.affiliation: Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Fei
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Guang-Yuan
authors.initials: GY
authors.lastname: Sun
authors.affiliation: Department of Vascular Gland,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Lei
authors.initials: L
authors.lastname: Han
authors.affiliation: Department of Ultrasound,the First Affiliated Hospital of Hebei North University, Zhangjiakou,Hebei 075000,China.
authors.forename: Rui-Min
authors.initials: RM
authors.lastname: Yang
mesh_terms: D000230:Adenocarcinoma / Q000098:blood supply / Q000378:metabolism*; D015179:Colorectal Neoplasms / Q000098:blood supply / Q000378:metabolism*; D018450:Disease Progression; D006801:Humans; D007150:Immunohistochemistry; D051798:Inhibitor of Differentiation Protein 1 / Q000378:metabolism*; D008113:Liver Neoplasms; D008207:Lymphatic Metastasis; D020780:Matrix Metalloproteinase 9 / Q000378:metabolism*; D008833:Microcirculation; D055806:Microvessels; D009389:Neovascularization, Pathologic
chemical_list: C497453:ID1 protein, human; D051798:Inhibitor of Differentiation Protein 1; C579270:MMP9 protein, human; D020780:Matrix Metalloproteinase 9