title: First description of Leishmania (Viannia) infection in Evandromyia saulensis, Pressatia sp. and Trichophoromyia auraensis (Psychodidae: Phlebotominae) in a transmission area of cutaneous leishmaniasis in Acre state, Amazon Basin, Brazil.
abstract: Studies on the sandfly fauna to evaluate natural infection indexes are still limited in the Brazilian Amazon, a region with an increasing incidence of cutaneous leishmaniasis. Here, by using a multiplex polymerase chain reaction directed to Leishmania kDNA and hybridisation, we were able to identify L. (Viannia) subgenus in 12 out of 173 sandflies captured in the municipality of Rio Branco, Acre state, revealing a positivity of 6.94%. By sequencing the Leishmania 234 bp-hsp70 amplified products from positive samples, infection by L. (V.) braziliensis was confirmed in five sandflies: one Evandromyia saulensis, three Trichophoromyia auraensis and one Pressatia sp. The finding of L. (Viannia) DNA in two Ev. saulensis corresponds to the first record of possible infection associated with this sandfly. Moreover, our study reveals for the first time in Brazil, Th. auraensis and Pressatia sp. infected by L. (Viannia) parasites.
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
authors.forename: Thais de
authors.initials: T
authors.lastname: Araujo-Pereira
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
authors.forename: Daniela de
authors.initials: D
authors.lastname: Pita-Pereira
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Pesquisa em Leishmaniose, Rio de Janeiro, RJ, Brasil.
authors.forename: Mariana Côrtes
authors.initials: MC
authors.lastname: Boité
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
authors.forename: Myllena
authors.initials: M
authors.lastname: Melo
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
authors.forename: Taiana Amancio da
authors.initials: TA
authors.lastname: Costa-Rego
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Doenças Parasitárias, Rio de Janeiro, RJ, Brasil.
authors.forename: Andressa Alencastre
authors.initials: AA
authors.lastname: Fuzari
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Doenças Parasitárias, Rio de Janeiro, RJ, Brasil.
authors.forename: Reginaldo Peçanha
authors.initials: RP
authors.lastname: Brazil
authors.affiliation: Fundação Oswaldo Cruz-Fiocruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular e Doenças Endêmicas, Rio de Janeiro, RJ, Brasil.
authors.forename: Constança
authors.initials: C
authors.lastname: Britto
mesh_terms: D000818:Animals; D001938:Brazil; D016054:DNA, Protozoan / Q000235:genetics; D007303:Insect Vectors / Q000145:classification / Q000469:parasitology*; D007891:Leishmania / Q000145:classification* / Q000302:isolation & purification; D016773:Leishmaniasis, Cutaneous / Q000635:transmission*; D060885:Multiplex Polymerase Chain Reaction; D011576:Psychodidae / Q000145:classification / Q000469:parasitology*
chemical_list: D016054:DNA, Protozoan