title: H19 non-coding RNA in urine cells detects urothelial carcinoma: a pilot study.
abstract: BACKGROUND
  Urothelial carcinoma (UC) is common and highly recurrent. Diagnosis and follow-up involve invasive cystoscopies.
  
  
  OBJECTIVE
  To evaluate H19 RNA in urine cells as diagnostic tool for UC.
  
  
  METHODS
  RT-PCR analysis of urine samples from healthy volunteers and UC patients.
  
  
  RESULTS
  H19 RNA was unequivocally detected in the urine of 90.5% of patients and 25.9% of controls. H19 copies were three orders of magnitude higher in patients. Receiver operating characteristic analysis showed an area under the curve of 0.933.
  
  
  CONCLUSIONS
  This pilot study shows that urinary cell H19 is a highly sensitive test for UC and pending verification could transform patient management.
authors.affiliation: a Department of Urology , Hadassah - Hebrew University Medical Centre , Jerusalem , Israel.
authors.forename: Ilan
authors.initials: I
authors.lastname: Gielchinsky
authors.affiliation: b Department of Biological Chemistry , Institute of Life Sciences, the Hebrew University , Jerusalem , Israel.
authors.forename: Michal
authors.initials: M
authors.lastname: Gilon
authors.affiliation: b Department of Biological Chemistry , Institute of Life Sciences, the Hebrew University , Jerusalem , Israel.
authors.forename: Rasha
authors.initials: R
authors.lastname: Abu-Lail
authors.affiliation: b Department of Biological Chemistry , Institute of Life Sciences, the Hebrew University , Jerusalem , Israel.
authors.forename: Imad
authors.initials: I
authors.lastname: Matouk
authors.affiliation: b Department of Biological Chemistry , Institute of Life Sciences, the Hebrew University , Jerusalem , Israel.
authors.forename: Avraham
authors.initials: A
authors.lastname: Hochberg
authors.affiliation: a Department of Urology , Hadassah - Hebrew University Medical Centre , Jerusalem , Israel.
authors.forename: Ofer N
authors.initials: ON
authors.lastname: Gofrit
authors.affiliation: c Nephrology and Hypertension Services , Hadassah - Hebrew University Medical Centre , Jerusalem , Israel.
authors.forename: Iddo Z
authors.identifier: http://orcid.org/0000-0002-9180-3604
authors.initials: IZ
authors.lastname: Ben-Dov
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D014408:Biomarkers, Tumor / Q000652:urine; D016022:Case-Control Studies; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010865:Pilot Projects; D016133:Polymerase Chain Reaction; D062085:RNA, Long Noncoding / Q000652:urine*; D012372:ROC Curve; D014571:Urologic Neoplasms / Q000175:diagnosis* / Q000652:urine; D019459:Urothelium / Q000473:pathology*
chemical_list: D014408:Biomarkers, Tumor; C094335:H19 long non-coding RNA; D062085:RNA, Long Noncoding
keywords: Non-coding RNA; bladder cancer; diagnostic biomarkers; urine biomarkers