title: Viral effector protein manipulates host hormone signaling to attract insect vectors.
abstract: Some plant and animal pathogens can manipulate their hosts to cause them to release odors that are attractive to the pathogens' arthropod vectors. However, the molecular mechanism underlying this process is largely unexplored, and the specific effectors the pathogens employ as well as the pathways within the hosts they target are currently unknown. Here we reveal that the aphid-borne cucumber mosaic virus (CMV) employs its 2b protein, a well-characterized viral suppressor of host RNA interference (VSR), to target the host's jasmonate (JA) hormone pathway, thus acting as a viral inducer of host attractiveness to insect vectors (VIA). 2b inhibits JA signaling by directly interacting with and repressing JA-induced degradation of host jasmonate ZIM-domain proteins, instead of using its VSR activity. Our findings identify a previously defined VSR protein as a VIA and uncover a molecular mechanism CMV uses to manipulate host's attractiveness to insect vectors by targeting host hormone signaling.
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Dewei
authors.initials: D
authors.lastname: Wu
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Tiancong
authors.initials: T
authors.lastname: Qi
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, USA.
authors.forename: Wan-Xiang
authors.initials: WX
authors.lastname: Li
authors.affiliation: College of Bioscience and Biotechnology, Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Haixia
authors.initials: H
authors.lastname: Tian
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Hua
authors.initials: H
authors.lastname: Gao
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Jiaojiao
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Ge
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Ruifeng
authors.initials: R
authors.lastname: Yao
authors.affiliation: College of Bioscience and Biotechnology, Crop Gene Engineering Key Laboratory of Hunan Province, Hunan Agricultural University, Changsha, Hunan 410128, China.
authors.forename: Chunmei
authors.initials: C
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
authors.forename: Xian-Bing
authors.initials: XB
authors.lastname: Wang
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Yule
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
authors.forename: Le
authors.initials: L
authors.lastname: Kang
authors.affiliation: Department of Plant Pathology and Microbiology, University of California, Riverside, CA 92521, USA.
authors.forename: Shou-Wei
authors.initials: SW
authors.lastname: Ding
authors.affiliation: Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University, Beijing 100084, China.
authors.forename: Daoxin
authors.initials: D
authors.lastname: Xie
mesh_terms: D000818:Animals; D001042:Aphids / Q000821:virology; D017360:Arabidopsis / Q000235:genetics / Q000469:parasitology* / Q000821:virology; D003517:Cyclopentanes / Q000494:pharmacology; D006790:Host-Parasite Interactions / Q000187:drug effects; D007303:Insect Vectors / Q000502:physiology*; D054883:Oxylipins / Q000494:pharmacology; D010937:Plant Growth Regulators / Q000378:metabolism*; D011485:Protein Binding / Q000187:drug effects; D059748:Proteolysis / Q000187:drug effects; D034622:RNA Interference; D015398:Signal Transduction; D014764:Viral Proteins / Q000378:metabolism*
chemical_list: C404227:2b protein, cucumber mosaic virus; D003517:Cyclopentanes; D054883:Oxylipins; D010937:Plant Growth Regulators; D014764:Viral Proteins; C011006:jasmonic acid