title: Physical properties and biological interactions of liposomes developed as a drug carrier in the field of regenerative medicine.
abstract: Liposomes are used for encapsulation of the active compounds in different therapies, with the increasing frequency. The important areas of clinical applications of liposomes are cancer targeted treatment, antibiotic delivery or regenerative medicine. The liposomes can transfer both hydrophilic and hydrophobic compounds and have the lipid bilayer which imitates the cell membrane. Liposomes additionally may extend half-live period of drugs and protect them against the elimination in different ways, such as phagocytosis, enzymatic cleavage or exclusion by detoxification. The size and charge of liposomes play an important role in drug distribution and absorption into the cell. Limited data is available on the effects of liposomes on stem cells and progenitor cells. In this article, we examined the effect of charged conventional liposomes on growth of mesenchymal and blood stem cells isolated from umbilical cord. The data suggest a likelihood, that positively charged liposomes could impair stem cell growth and metabolism. Different methodological approaches allowed for the selection of negatively charged liposomes for further experiments, as the only type of liposomes which has the lowest cytotoxicity and does not affect hematopoietic cell proliferation.
authors.affiliation: a Department of Regenerative Medicine , Military Institute of Hygiene and Epidemiology , Warsaw , Poland.
authors.forename: Sławomir
authors.initials: S
authors.lastname: Lewicki
authors.affiliation: a Department of Regenerative Medicine , Military Institute of Hygiene and Epidemiology , Warsaw , Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Leśniak
authors.affiliation: b Department of Cellular Engineering , The Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology , Warsaw , Poland.
authors.forename: Eugeniusz Krzysztof
authors.initials: EK
authors.lastname: Machaj
authors.affiliation: c Department of Microbiology , Military Institute of Hygiene and Epidemiology , Warsaw , Poland , and.
authors.forename: Małgorzata
authors.initials: M
authors.lastname: Antos-Bielska
authors.affiliation: d Military Institute of Technology, Biomedical Engineering Center, Institute of Optoelectronics , Warsaw , Poland.
authors.forename: Elżbieta Anna
authors.initials: EA
authors.lastname: Trafny
authors.affiliation: a Department of Regenerative Medicine , Military Institute of Hygiene and Epidemiology , Warsaw , Poland.
authors.forename: Janusz
authors.initials: J
authors.lastname: Kocik
authors.affiliation: b Department of Cellular Engineering , The Maria Skłodowska-Curie Memorial Cancer Center and Institute of Oncology , Warsaw , Poland.
authors.forename: Zygmunt
authors.initials: Z
authors.lastname: Pojda
mesh_terms: D049109:Cell Proliferation; D002470:Cell Survival / Q000187:drug effects; D004337:Drug Carriers / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D005260:Female; D006412:Hematopoietic Stem Cells / Q000187:drug effects*; D006801:Humans; D008081:Liposomes / Q000138:chemical synthesis / Q000737:chemistry / Q000494:pharmacology; D059630:Mesenchymal Stem Cells / Q000187:drug effects*; D044968:Regenerative Medicine
chemical_list: D004337:Drug Carriers; D008081:Liposomes
keywords: Cord blood hematopoietic stem cells; liposomes; thin-lipid film hydration method; umbical cord stem cells (UCSC); wound healing