title: Xylanases of Cellulomonas flavigena: expression, biochemical characterization, and biotechnological potential.
abstract: Four xylanases of Cellulomonas flavigena were cloned, expressed in Escherichia coli and purified. Three enzymes (CFXyl1, CFXyl2, and CFXyl4) were from the GH10 family, while CFXyl3 was from the GH11 family. The enzymes possessed moderate temperature stability and a neutral pH optimum. The enzymes were more stable at alkaline pH values. CFXyl1 and CFXyl2 hydrolyzed xylan to form xylobiose, xylotriose, xylohexaose, xylopentaose, and xylose, which is typical for GH10. CFXyl3 (GH11) and CFXyl4 (GH10) formed the same xylooligosaccharides, but xylose was formed in small amounts. The xylanases made efficient saccharification of rye, wheat and oat, common components of animal feed, which indicates their high biotechnological potential.
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia. ssl204@rambler.ru.
authors.forename: Alexander V
authors.initials: AV
authors.lastname: Lisov
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Oksana V
authors.initials: OV
authors.lastname: Belova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Zoya A
authors.initials: ZA
authors.lastname: Lisova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Nataliy G
authors.initials: NG
authors.lastname: Vinokurova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Alexey S
authors.initials: AS
authors.lastname: Nagel
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Zhanna I
authors.initials: ZI
authors.lastname: Andreeva-Kovalevskaya
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Zhanna I
authors.initials: ZI
authors.lastname: Budarina
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Maxim O
authors.initials: MO
authors.lastname: Nagornykh
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Marina V
authors.initials: MV
authors.lastname: Zakharova
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Andrey M
authors.initials: AM
authors.lastname: Shadrin
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.|Pushchino State Institute of Life Sciences, 3 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Alexander S
authors.initials: AS
authors.lastname: Solonin
authors.affiliation: G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences (IBPM RAS), 5 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.|Pushchino State Institute of Life Sciences, 3 Prospekt Nauki, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Alexey A
authors.initials: AA
authors.lastname: Leontievsky
keywords: Cellulomonas flavigena; Saccharification of cereals; Xylanases; Xylooligosaccharides