title: Enhanced wound healing activity of desert locust (Schistocerca gregaria) vs. shrimp (Penaeus monodon) chitosan based scaffolds.
abstract: Chitosan (CS) has received great attention in tissue engineering, especially in wound healing acceleration. In this study, chitin was isolated from desert locust (Schistocerca gregaria) and shrimp (Penaeus monodon) then deacetylated to chitosan. Then, chitosan was characterized by degree of deacetylation (DD), molecular weight (M.Wt), swelling index (SI), Fourier transform infrared (FTIR) and X ray diffraction (XRD). The chitosan was then casted into 2D scaffolds and was pictured using scanning electron microscope (SEM). In a comparative study, primary cell cultures of neonatal (1-2day old) mice skin tissue, supplemented with 10% fetal calf serum, were seeded onto locust chitosan based scaffolds (LCSBS) and shrimp chitosan based scaffold (SCSBS). Their attachment percentage was determined after 1h. The cell proliferation rate was tested for 5days on LCSBS and SCSBS. Wound healing activity progress of LCSBS and SCSBS was tested in vivo using histopathology, and results revealed that seeded and unseeded LCSBS accelerated healing in contrast to SCSBS. The data demonstrated that LCSBS shows a high degree of biocompatibility in vivo. These results suggest that LCSBS is a potential substitute for the development of low cost implantable materials to accelerate wound healing.
authors.affiliation: Nanotechnology Program, School of Sciences and Engineering, American University in Cairo, New Cairo, Egypt.
authors.forename: Narguess H
authors.initials: NH
authors.lastname: Marei
authors.affiliation: Research and Development Unit, Holding Company for Biological products and Vaccines-VACSERA, Giza, Egypt.
authors.forename: W
authors.initials: W
authors.lastname: El-Mazny
authors.affiliation: Research and Development Unit, Holding Company for Biological products and Vaccines-VACSERA, Giza, Egypt.
authors.forename: Aida
authors.initials: A
authors.lastname: El-Shaer
authors.affiliation: Biotechnology Program, Faculty of Sciences, Cairo University, Giza, Egypt.
authors.forename: Kareem Dorri
authors.initials: KD
authors.lastname: Zaki
authors.affiliation: Faculty of Biotechnology, Modern Sciences and Arts University, 6th of October, Egypt.
authors.forename: Zahra S
authors.initials: ZS
authors.lastname: Hussein
authors.affiliation: Entomology Department, Faculty of Sciences, Cairo University, Giza, Egypt. Electronic address: emtithal_a@yahoo.com.
authors.forename: Emtithal M
authors.initials: EM
authors.lastname: Abd-El-Samie
mesh_terms: D000818:Animals; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D048271:Chitosan / Q000494:pharmacology*; D006110:Grasshoppers / Q000737:chemistry*; D051379:Mice; D033561:Penaeidae / Q000737:chemistry*; D016062:Porosity; D012867:Skin / Q000166:cytology / Q000187:drug effects; D054457:Tissue Scaffolds / Q000737:chemistry*; D014945:Wound Healing / Q000187:drug effects*
chemical_list: D048271:Chitosan
keywords: Chitosan; Tissue engineering; Wound healing