title: Sequence-Controlled Delivery of Peptides from Hierarchically Structured Nanomaterials.
abstract: Peptide drugs delivered orally need to be protected from degradation for achieving their functions. To fulfill the complicated task of oral drug delivery, we present a hierarchically structured drug-delivery system that can undertake structural changes, so multiple functions can be triggered by a sequence of stimuli. Such hierarchical system is achieved in a nanoparticle-in-nanofiber configuration, in which both the nanofibers and the nanoparticles are pH-responsive and biocompatible. A model peptide is efficiently encapsulated under mild condition, and the nanocarriers are further electrospun with a pH-responsive mucoadhesive polymer. The nanoparticles are released from the nanofibers, and thereafter the peptides are released from the nanoparticles in a pH-responsive manner. The nanoparticles are compatible with caco-2 cells, and the endocytosis of the nanoparticles is described in detail.
authors.affiliation: Max Planck Institute for Polymer Research , Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Carl Wei
authors.initials: CW
authors.lastname: He
authors.affiliation: Max Planck Institute for Polymer Research , Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Maria
authors.initials: M
authors.lastname: Parowatkin
authors.affiliation: Max Planck Institute for Polymer Research , Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Volker
authors.initials: V
authors.lastname: Mailänder
authors.forename: Marion
authors.initials: M
authors.lastname: Flechtner-Mors
authors.forename: Ulrich
authors.identifier: http://orcid.org/0000-0001-7582-1143
authors.initials: U
authors.lastname: Ziener
authors.affiliation: Max Planck Institute for Polymer Research , Ackermannweg 10, 55128 Mainz, Germany.
authors.forename: Katharina
authors.initials: K
authors.lastname: Landfester
authors.affiliation: Max Planck Institute for Polymer Research , Ackermannweg 10, 55128 Mainz, Germany.|Department of Materials Science and Engineering, School of Molecular Science and Engineering, Vidyasirimedhi Institute of Science and Technology , 21210 Rayong, Thailand.
authors.forename: Daniel
authors.identifier: http://orcid.org/0000-0002-6023-703X
authors.initials: D
authors.lastname: Crespy
keywords: glycol chitosan; hierarchical nanostructures; interfacial cross-linking; miniemulsion; nanoconfinement; polyelectrolyte complexation; protein drug delivery