title: B-lymphocytes forming follicle-like structures in the lung tissue of tuberculosis-infected mice: Dynamics, phenotypes and functional activity.
abstract: During tuberculosis (TB) infection, B cells form follicles in close vicinity of lung granuloma. We assessed the dynamics of follicle formation, surface phenotypes and functional activity of lung B cells during TB course in genetically susceptible mice. The follicles appeared early post infection and peaked at weeks 7-8. Lung B cells resembled classical B2 cells (CD19+IgMloIgDhiCD1d-CD21/35intCD5-CD11b-CD43-), but differed from them by the absence of B2 marker CD23. Lung B-cells constitutively expressed MHC II molecules, presented mycobacterial antigens to immune CD4+T-cells and produced high amounts of IL-6 and IL-11, but no classical type 1 (TNF-α, IFN-γ), or anti-inflammatory (IL-10, TGF-β) cytokines. The total antibody response in tuberculous lung showed almost no specificity to mycobacteria. A panel of monoclonal antibodies obtained from lung B cells contained only few clones with reactivity to mycobacteria. Our results suggest that anti-TB B cell response in the lung has clear pathological and doubtful protective role.
authors.affiliation: Department of Immunology, Central Institute for Tuberculosis, Moscow, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Linge
authors.affiliation: Department of Immunology, Central Institute for Tuberculosis, Moscow, Russia.
authors.forename: Alexander
authors.initials: A
authors.lastname: Dyatlov
authors.affiliation: Department of Immunology, Central Institute for Tuberculosis, Moscow, Russia.
authors.forename: Elena
authors.initials: E
authors.lastname: Kondratieva
authors.affiliation: Department of Immunology, Central Institute for Tuberculosis, Moscow, Russia.
authors.forename: Vadim
authors.initials: V
authors.lastname: Avdienko
authors.affiliation: Department of Immunology, Central Institute for Tuberculosis, Moscow, Russia; Department of Immunology, School of Biology, Moscow State University, Moscow, Russia. Electronic address: asapt@aha.ru.
authors.forename: Alexander
authors.initials: A
authors.lastname: Apt
authors.affiliation: Department of Immunology, Central Institute for Tuberculosis, Moscow, Russia.
authors.forename: Tatiana
authors.initials: T
authors.lastname: Kondratieva
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal / Q000096:biosynthesis / Q000276:immunology; D000942:Antigens, Bacterial / Q000276:immunology; D001402:B-Lymphocytes / Q000276:immunology*; D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D016207:Cytokines / Q000096:biosynthesis; D005260:Female; D016130:Immunophenotyping; D008168:Lung / Q000276:immunology*; D008815:Mice, Inbred Strains; D009169:Mycobacterium tuberculosis / Q000276:immunology; D010994:Pleura / Q000276:immunology; D014397:Tuberculosis, Pulmonary / Q000276:immunology*
chemical_list: D000911:Antibodies, Monoclonal; D000942:Antigens, Bacterial; D016207:Cytokines
keywords: B-lymphocytes; Lung tissue; Monoclonal antibodies; Surface phenotype; Tuberculosis