title: Expression of the UVR8 photoreceptor in different tissues reveals tissue-autonomous features of UV-B signalling.
abstract: The Arabidopsis UV-B photoreceptor UV RESISTANCE LOCUS 8 (UVR8) orchestrates the expression of hundreds of genes, many of which can be associated with UV-B tolerance. UV-B does not efficiently penetrate into tissues, yet UV-B regulates complex growth and developmental responses. To unravel to what extent and how UVR8 located in different tissues contributes to UV-B-induced responses, we expressed UVR8 fused to the YELLOW FLUORESCENT PROTEIN (YFP) under the control of tissue-specific promoters in a uvr8 null mutant background. We show that (1) UVR8 localized in the epidermis plays a major role in regulating cotyledon expansion, and (2) expression of UVR8 in the mesophyll is important to protect adult plants from the damaging effects of UV-B. We found that UV-B induces transcription of selected genes, including the key transcriptional regulator ELONGATED HYPOCOTYL 5 (HY5), only in tissues that express UVR8. Thus, we suggest that tissue-autonomous and simultaneous UVR8 signalling in different tissues mediates, at least partly, developmental and defence responses to UV-B.
authors.affiliation: Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62, H-6726, Szeged, Hungary.
authors.forename: Péter
authors.initials: P
authors.lastname: Bernula
authors.affiliation: Department of Botany and Plant Biology, Sciences III, University of Geneva, CH-1211, Geneva 4, Switzerland.
authors.forename: Carlos Daniel
authors.initials: CD
authors.lastname: Crocco
authors.affiliation: Department of Botany and Plant Biology, Sciences III, University of Geneva, CH-1211, Geneva 4, Switzerland.
authors.forename: Adriana Beatriz
authors.initials: AB
authors.lastname: Arongaus
authors.affiliation: Department of Botany and Plant Biology, Sciences III, University of Geneva, CH-1211, Geneva 4, Switzerland.
authors.forename: Roman
authors.initials: R
authors.lastname: Ulm
authors.affiliation: Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62, H-6726, Szeged, Hungary.|Institute of Molecular Plant Science, School of Biological Sciences, University of Edinburgh, Edinburgh, EH9 3JH, UK.
authors.forename: Ferenc
authors.initials: F
authors.lastname: Nagy
authors.affiliation: Institute of Plant Biology, Biological Research Centre, Temesvári krt. 62, H-6726, Szeged, Hungary.
authors.forename: András
authors.identifier: http://orcid.org/0000-0003-2055-3430
authors.initials: A
authors.lastname: Viczián
mesh_terms: D000064:Acclimatization; D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism / Q000528:radiation effects*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D050976:Basic-Leucine Zipper Transcription Factors / Q000235:genetics / Q000378:metabolism; D002868:Chromosomal Proteins, Non-Histone / Q000235:genetics / Q000378:metabolism*; D005419:Flavonoids / Q000378:metabolism; D018506:Gene Expression Regulation, Plant; D018546:Hypocotyl / Q000254:growth & development / Q000378:metabolism; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D058503:Mesophyll Cells / Q000378:metabolism; D009154:Mutation; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D055519:Photoreceptors, Plant / Q000235:genetics / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic; D036226:Seedlings / Q000235:genetics / Q000378:metabolism; D015398:Signal Transduction; D014466:Ultraviolet Rays
chemical_list: D029681:Arabidopsis Proteins; D001426:Bacterial Proteins; D050976:Basic-Leucine Zipper Transcription Factors; D002868:Chromosomal Proteins, Non-Histone; D005419:Flavonoids; C109254:HY5 protein, Arabidopsis; D008164:Luminescent Proteins; D009687:Nuclear Proteins; D055519:Photoreceptors, Plant; C525052:Uvr8 protein, Arabidopsis; C054562:yellow fluorescent protein, Bacteria
keywords: Arabidopsis; tissue specificity; ultraviolet-B