title: Surgical Denervation in the Imiquimod-Induced Psoriasiform Mouse Model.
abstract: Psoriasis is a chronic inflammatory skin disease with a prevalence of 2-3 %. It appears to result from a combination of genetic and environmental factors, but the precise pathogenesis is still unknown. Neurogenic inflammation is involved in psoriasis pathogenesis as well, but the role of neurogenic factors is currently unclear. Molecular studies often involve material obtained from patients. However, many questions and especially experimental manipulations are not suited for study in humans. Imiquimod application on mouse skin leads to immune cell infiltration, inflammation with intense redness, epidermal thickening, and scaling that jointly greatly resembles human psoriasis. Here we describe the use of surgical denervation in the imiquimod-induced psoriasiform model, to study the role of skin innervation and neuropeptides in the pathogenesis of psoriasis.
authors.affiliation: Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Armanda J
authors.initials: AJ
authors.lastname: Onderdijk
authors.affiliation: Department of Plastic Surgery, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Ineke M
authors.initials: IM
authors.lastname: Hekking-Weijma
authors.affiliation: Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands.
authors.forename: Edwin F
authors.initials: EF
authors.lastname: Florencia
authors.affiliation: Department of Dermatology, Erasmus University Medical Center, Rotterdam, The Netherlands. e.prens@erasmusmc.nl.
authors.forename: Errol P
authors.initials: EP
authors.lastname: Prens
mesh_terms: D000634:Aminoquinolines / Q000009:adverse effects; D000818:Animals; D003714:Denervation / Q000295:instrumentation / Q000379:methods*; D062109:Dermatologic Surgical Procedures / Q000295:instrumentation / Q000379:methods*; D004195:Disease Models, Animal; D005260:Female; D005786:Gene Expression Regulation; D006801:Humans; D000077271:Imiquimod; D020381:Interleukin-17 / Q000235:genetics / Q000276:immunology; D053759:Interleukin-23 / Q000235:genetics / Q000276:immunology; D008562:Membrane Glycoproteins / Q000235:genetics / Q000276:immunology; D051379:Mice; D008810:Mice, Inbred C57BL; D011565:Psoriasis / Q000139:chemically induced / Q000235:genetics / Q000276:immunology* / Q000473:pathology; D016692:Receptors, Antigen, T-Cell, gamma-delta / Q000235:genetics / Q000276:immunology; D012867:Skin / Q000187:drug effects* / Q000276:immunology / Q000294:innervation; D013601:T-Lymphocytes / Q000187:drug effects* / Q000276:immunology; D051199:Toll-Like Receptor 7 / Q000235:genetics / Q000276:immunology
chemical_list: D000634:Aminoquinolines; D020381:Interleukin-17; D053759:Interleukin-23; D008562:Membrane Glycoproteins; D016692:Receptors, Antigen, T-Cell, gamma-delta; C477038:Tlr7 protein, mouse; D051199:Toll-Like Receptor 7; D000077271:Imiquimod
keywords: (γδ) T cells; Denervation; IL-17; IL-23; Imiquimod; Mouse model; Nerves; Neuropeptides; Psoriasis; TLR7