title: Generation of a Floxed Allele of the Mouse MicroRNA-200 Clusters.
abstract: MicroRNA-8 and its target gene, u-shaped (ush), regulate body size in Drosophila. As the mouse homolog of the Drosophila miR-8, whether the miR-200 family has similar functions and how they perform their regulatory roles in body size control is unknown. In order to discover the biological function of the miR-200s in vivo, we generated mice lacking the miR-200b/200a/429 gene cluster using the Cre/loxp system. miR-200b/200a/429 null mutant mice were viable, fertile, and showed normal development.
authors.affiliation: Key Laboratory of Animal Embryo & Molecular Breeding of Hubei Province, Institute of Veterinary and Animal Science, Hubei Academy of Agricultural Science, Wuhan, 430064, China.
authors.forename: Hongyan
authors.initials: H
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Tao
authors.affiliation: State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
authors.forename: Kui
authors.initials: K
authors.lastname: Li
authors.affiliation: Key Laboratory of Animal Embryo & Molecular Breeding of Hubei Province, Institute of Veterinary and Animal Science, Hubei Academy of Agricultural Science, Wuhan, 430064, China.
authors.forename: Yanzhen
authors.initials: Y
authors.lastname: Bi
authors.affiliation: Key Laboratory of Animal Embryo & Molecular Breeding of Hubei Province, Institute of Veterinary and Animal Science, Hubei Academy of Agricultural Science, Wuhan, 430064, China. renhongyan507@163.com.
authors.forename: Xinmin
authors.initials: X
authors.lastname: Zheng
mesh_terms: D000483:Alleles; D000818:Animals; D004331:Drosophila melanogaster; D017353:Gene Deletion; D019426:Integrases / Q000235:genetics / Q000378:metabolism; D051379:Mice; D018345:Mice, Knockout; D035683:MicroRNAs / Q000235:genetics*; D005810:Multigene Family
chemical_list: D035683:MicroRNAs; C543719:Mirn200 microRNA, mouse; C045073:Cre recombinase; D019426:Integrases
keywords: Cre-loxp; Gene targeting; MicroRNA-200s