title: Cisplatin-induced self-assembly of graphene oxide sheets into spherical nanoparticles for damaging sub-cellular DNA.
abstract: This report describes the hitherto unobserved cisplatin induced self-assembly of 2D-graphene oxide sheets into 3D-spherical nano-scale particles. These nanoparticles can encompass dual DNA damaging drugs simultaneously. A combination of confocal microscopy, gel electrophoresis and flow cytometry studies clearly demonstrated that these novel nanoparticles can internalize into cancer cells by endocytosis, localize into lysosomes, and damage DNA, leading to apoptosis. Cell viability assays indicated that these nanoparticles were more cytotoxic towards cancer cells compared to healthy cells.
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Pune, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India. sudipta.basu@iiserpune.ac.in.
authors.forename: Aditi
authors.initials: A
authors.lastname: Nandi
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Pune, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India. sudipta.basu@iiserpune.ac.in.
authors.forename: Abhik
authors.initials: A
authors.lastname: Mallick
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Pune, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India. sudipta.basu@iiserpune.ac.in.
authors.forename: Piyush
authors.initials: P
authors.lastname: More
authors.affiliation: Academy of Scientific & Innovative Research (AcSIR), Physical Chemistry Division, CSIR National Chemical Laboratory, Dr. Homi Bhabha Road, Pune 411008, Maharashtra, India.
authors.forename: Poulomi
authors.initials: P
authors.lastname: Sengupta
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Pune, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India. sudipta.basu@iiserpune.ac.in.
authors.forename: Nirmalya
authors.initials: N
authors.lastname: Ballav
authors.affiliation: Department of Chemistry, Indian Institute of Science Education and Research (IISER) Pune, Dr. Homi Bhabha Road, Pashan, Pune, Maharashtra 411008, India. sudipta.basu@iiserpune.ac.in.
authors.forename: Sudipta
authors.identifier: http://orcid.org/0000-0002-0433-8899
authors.initials: S
authors.lastname: Basu
mesh_terms: D000970:Antineoplastic Agents / Q000737:chemistry* / Q000494:pharmacology; D016923:Cell Death / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D002945:Cisplatin / Q000737:chemistry* / Q000494:pharmacology; D004249:DNA Damage; D004305:Dose-Response Relationship, Drug; D006108:Graphite / Q000737:chemistry*; D006367:HeLa Cells; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010087:Oxides / Q000138:chemical synthesis* / Q000737:chemistry; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D010087:Oxides; D006108:Graphite; D002945:Cisplatin