title: Shifting molecular localization by plasmonic coupling in a single-molecule mirage.
abstract: Over the last decade, two fields have dominated the attention of sub-diffraction photonics research: plasmonics and fluorescence nanoscopy. Nanoscopy based on single-molecule localization offers a practical way to explore plasmonic interactions with nanometre resolution. However, this seemingly straightforward technique may retrieve false positional information. Here, we make use of the DNA origami technique to both control a nanometric separation between emitters and a gold nanoparticle, and as a platform for super-resolution imaging based on single-molecule localization. This enables a quantitative comparison between the position retrieved from single-molecule localization, the true position of the emitter and full-field simulations. We demonstrate that plasmonic coupling leads to shifted molecular localizations of up to 30 nm: a single-molecule mirage.
authors.affiliation: Institute for Physical &Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Rebenring 56, 38106 Braunschweig, Germany.
authors.forename: Mario
authors.initials: M
authors.lastname: Raab
authors.affiliation: Institute for Physical &Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Rebenring 56, 38106 Braunschweig, Germany.
authors.forename: Carolin
authors.initials: C
authors.lastname: Vietz
authors.affiliation: Centro de Investigaciones en Bionanociencias (CIBION), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Godoy Cruz 2390, C1425FQD Ciudad de Buenos Aires, Argentina.|Departamento de Física, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Güiraldes 2620, C1428EAH Ciudad de Buenos Aires, Argentina.
authors.forename: Fernando Daniel
authors.initials: FD
authors.lastname: Stefani
authors.affiliation: Institute for Physical &Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Rebenring 56, 38106 Braunschweig, Germany.
authors.forename: Guillermo Pedro
authors.initials: GP
authors.lastname: Acuna
authors.affiliation: Institute for Physical &Theoretical Chemistry, and Braunschweig Integrated Centre of Systems Biology (BRICS), and Laboratory for Emerging Nanometrology (LENA), Braunschweig University of Technology, Rebenring 56, 38106 Braunschweig, Germany.
authors.forename: Philip
authors.initials: P
authors.lastname: Tinnefeld