title: Silibinin-loaded magnetic nanoparticles inhibit hTERT gene expression and proliferation of lung cancer cells.
abstract: Nanoparticle-based targeted drug delivery has the potential for rendering silibinin specifically at the favorite site using an external magnetic field. Also, it can circumvent the pitfalls of poor solubility. For this purpose, silibinin-loaded magnetic nanoparticles are fabricated, characterized and evaluated cytotoxicity and hTERT gene expression in A549 lung cancer cell line. silibinin-loaded PLGA-PEG-Fe3O4had dose- and time-dependent cytotoxicity than pure silibinin. Additionally, hTERT expression is more efficiently reduced with increasing concentrations of nanosilibinin than pure silibinin. The present study indicates that PLGA-PEG-Fe3O4nanoparticles, as an effective targeted carrier, can make a promising horizon in targeted lung cancer therapy.
authors.affiliation: a Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Soumaye
authors.initials: S
authors.lastname: Amirsaadat
authors.affiliation: a Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Younes
authors.initials: Y
authors.lastname: Pilehvar-Soltanahmadi
authors.affiliation: a Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Faraz
authors.initials: F
authors.lastname: Zarghami
authors.affiliation: b Molecular Medicine, Faculty of Advanced Medical Sciences , Tabriz university of Medical Sciences , Iran.
authors.forename: Shahriar
authors.initials: S
authors.lastname: Alipour
authors.affiliation: a Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Zohreh
authors.initials: Z
authors.lastname: Ebrahimnezhad
authors.affiliation: a Department of Medical Biotechnology, Faculty of Advanced Medical Sciences , Tabriz University of Medical Sciences , Tabriz , Iran.
authors.forename: Nosratollah
authors.initials: N
authors.lastname: Zarghami
mesh_terms: D000072283:A549 Cells; D049109:Cell Proliferation / Q000187:drug effects; D004337:Drug Carriers / Q000737:chemistry*; D065546:Drug Liberation; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects*; D006801:Humans; D019344:Lactic Acid / Q000737:chemistry; D008175:Lung Neoplasms / Q000473:pathology*; D058185:Magnetite Nanoparticles / Q000737:chemistry*; D011092:Polyethylene Glycols / Q000737:chemistry; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D000077385:Silybin; D012838:Silymarin / Q000737:chemistry* / Q000494:pharmacology*; D019098:Telomerase / Q000235:genetics*
chemical_list: D004337:Drug Carriers; D058185:Magnetite Nanoparticles; D012838:Silymarin; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; D011092:Polyethylene Glycols; D000077385:Silybin; C509186:TERT protein, human; D019098:Telomerase
keywords: A549; Fe3O4; PLGA-PEG; Silibinin; hTERT