title: Adeno-associated virus serotype rh.10 displays strong muscle tropism following intraperitoneal delivery.
abstract: Recombinant adeno-associated virus (rAAV) is an attractive tool for basic science and translational medicine including gene therapy, due to the versatility in its cell and organ transduction. Previous work indicates that rAAV transduction patterns are highly dependent on route of administration. Based on this relationship, we hypothesized that intraperitoneal (IP) administration of rAAV produces unique patterns of tissue tropism. To test this hypothesis, we investigated the transduction efficiency of 12 rAAV serotypes carrying an enhanced green fluorescent protein (EGFP) reporter gene in a panel of 12 organs after IP injection. Our data suggest that IP administration emphasizes transduction patterns that are different from previously reported intravascular delivery methods. Using this approach, rAAV efficiently transduces the liver, pancreas, skeletal muscle, heart and diaphragm without causing significant histopathological changes. Of note, rAAVrh.10 showed excellent muscle transduction following IP administration, highlighting its potential as a new muscle-targeting vector.
authors.affiliation: Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.|Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts, USA.|Department of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Ai
authors.affiliation: Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Jia
authors.initials: J
authors.lastname: Li
authors.affiliation: Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts, USA.|Department of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Dominic J
authors.initials: DJ
authors.lastname: Gessler
authors.affiliation: Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
authors.forename: Qin
authors.initials: Q
authors.lastname: Su
authors.affiliation: Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Institute of Urology, Department of Urology, West China Hospital, Sichuan University, Chengdu, Sichuan, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Li
authors.affiliation: Horae Gene Therapy Center, University of Massachusetts Medical School, Worcester, Massachusetts, USA.|Department of Microbiology and Physiology Systems, University of Massachusetts Medical School, Worcester, Massachusetts, USA.|State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, P.R. China.
authors.forename: Guangping
authors.initials: G
authors.lastname: Gao
mesh_terms: D000818:Animals; D000229:Dependovirus / Q000502:physiology*; D007274:Injections, Intraperitoneal; D008810:Mice, Inbred C57BL; D009132:Muscles / Q000821:virology*; D009928:Organ Specificity; D065288:Serogroup; D014161:Transduction, Genetic; D018523:Tropism / Q000502:physiology*