title: Calcium Detection in Secretion Granules of Avian Oviduct by Scanning Electron Microscopy (SEM) and Energy-dispersive X-Ray Microanalysis (EDX)1.
abstract: Secretion granules in the shell gland, isthmus, and albumin-secreting region of the hen oviduct were analyzed with WET-scanning electron microscopy (SEM) and EDX, a combination of wide-angle backscattered electron detector (BED) and energy-dispersive X-ray microanalyzer (EDX). Glutaraldehyde-fixed but unhydrated, unstained, and uncoated samples were analyzed; Ca was localized in all secretion granules in all three sections of the hen oviduct studied.
authors.affiliation: Department of Veterinary Anatomy (T.M; M.H.), Yamaguchi University, Yamaguchi City, and Department of Anatomy (Y.S.), Saitama Medical College, Japan (OA 82-281P1).
authors.forename: Takashi
authors.initials: T
authors.lastname: Makita
authors.affiliation: Department of Veterinary Anatomy (T.M; M.H.), Yamaguchi University, Yamaguchi City, and Department of Anatomy (Y.S.), Saitama Medical College, Japan (OA 82-281P1).
authors.forename: Masanori
authors.initials: M
authors.lastname: Hatsuoka
authors.affiliation: Department of Veterinary Anatomy (T.M; M.H.), Yamaguchi University, Yamaguchi City, and Department of Anatomy (Y.S.), Saitama Medical College, Japan (OA 82-281P1).
authors.forename: Yukiko
authors.initials: Y
authors.lastname: Sugi
keywords: Backscattered electron image; Calcium detection; Hydrated specimen; Secretion granules; WET-SEM; X-ray microanalysis.