title: Isoniazid: A Review of Characteristics, Properties and Analytical Methods.
abstract: Isoniazid is a synthetic antimicrobial and one of the most important first-line drugs used in the treatment of tuberculosis. Since it was introduced in the therapy in 1952, the drug remains at the front line of the antituberculosis treatment mainly due to its potency and high selectivity against Mycobacterium tuberculosis. Pharmaceutical analysis and therapeutic drug monitoring of isoniazid in both, pharmaceuticals and biological samples, plays an important role to comprehend aspects regarding to bioavailability, bioequivalence and therapeutic monitoring during patients following-up. In the last case, validated and simple methods are extremely useful for Public Healthy in order to guarantee the drug efficacy, safety and reduce the tuberculosis resistance. Among the available analytical tools, HPLC-based methods coupled to ultraviolet or mass spectroscopy are the most widely used techniques to quantify isoniazid. Therefore, this review highlights the main analytical methods reported in the literature for determination of isoniazid focusing in HPLC-based methods.
authors.affiliation: a Institute of Chemistry, São Paulo State University (UNESP) , Araraquara , Brazil.|b School of Pharmaceutical Sciences , São Paulo State University (UNESP) , Araraquara , Brazil.
authors.forename: Guilherme Felipe Dos Santos
authors.identifier: http://orcid.org/0000-0002-7644-5466
authors.initials: GFDS
authors.lastname: Fernandes
authors.affiliation: b School of Pharmaceutical Sciences , São Paulo State University (UNESP) , Araraquara , Brazil.
authors.forename: Hérida Regina Nunes
authors.initials: HRN
authors.lastname: Salgado
authors.affiliation: a Institute of Chemistry, São Paulo State University (UNESP) , Araraquara , Brazil.|b School of Pharmaceutical Sciences , São Paulo State University (UNESP) , Araraquara , Brazil.
authors.forename: Jean Leandro Dos
authors.initials: JLD
authors.lastname: Santos
mesh_terms: D000818:Animals; D000995:Antitubercular Agents / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D002623:Chemistry Techniques, Analytical / Q000379:methods*; D006801:Humans; D007538:Isoniazid / Q000032:analysis* / Q000493:pharmacokinetics / Q000494:pharmacology; D009169:Mycobacterium tuberculosis / Q000187:drug effects
chemical_list: D000995:Antitubercular Agents; D007538:Isoniazid
keywords: Analytical methods; high performance liquid chromatography; isoniazid; mass spectroscopy; ultraviolet