title: A bio-chemical application of N-GQDs and g-C3N4QDs sensitized TiO2nanopillars for the quantitative detection of pcDNA3-HBV.
abstract: Herein, TiO2nanopillars (NPs)/N-doped graphene quantum dots (N-GQDs)/g-C3N4QDs heterojunction efficiently suppressed the photogenerated charges recombination and improved photo-to-current conversion efficiency. The introduced N-GQDs and g-C3N4QDs could result in more effective separation of the photogenerated charges, and thus produce a further increase of the photocurrent. TiO2NPs/N-GQDs/g-C3N4QDs were firstly applied as the photoactive materials for the fabrication of the biosensors, and the primers of pcDNA3-HBV were then adsorbed on the TiO2NPs/N-GQDs/g-C3N4QDs modified electrode under the activation of EDC/NHS. With increase of the pcDNA3-HBV concentration, the photocurrent reduced once the double helix between the primers and pcDNA3-HBV formed. The developed photoelectrochemical (PEC) biosensor showed a sensitive response to pcDNA3-HBV in a linear range of 0.01 fmol/L to 20nmol/L with a detection limit of 0.005 fmol/L under the optimal conditions. The biosensor exhibited high sensitivity, good selectivity, good stability and reproducibility.
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, China.
authors.forename: Xuehui
authors.initials: X
authors.lastname: Pang
authors.affiliation: Department of Emergency, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Hongjun
authors.initials: H
authors.lastname: Bian
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, China.
authors.forename: Weijie
authors.initials: W
authors.lastname: Wang
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, China.
authors.forename: Malik Saddam
authors.initials: MS
authors.lastname: Khan
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, China.
authors.forename: Qiao
authors.initials: Q
authors.lastname: Wang
authors.affiliation: Central Laboratory, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China.
authors.forename: Jianni
authors.initials: J
authors.lastname: Qi
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, China. Electronic address: sdjndxwq@163.com.
authors.forename: Qin
authors.initials: Q
authors.lastname: Wei
authors.affiliation: Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong, University of Jinan, Jinan 250022, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Du
mesh_terms: D015374:Biosensing Techniques / Q000379:methods; D002460:Cell Line; D004279:DNA, Viral / Q000032:analysis* / Q000235:genetics; D055664:Electrochemical Techniques / Q000379:methods; D005822:Genetic Vectors / Q000032:analysis* / Q000235:genetics; D006108:Graphite / Q000737:chemistry*; D006509:Hepatitis B / Q000175:diagnosis / Q000821:virology; D006515:Hepatitis B virus / Q000235:genetics / Q000302:isolation & purification*; D006801:Humans; D057230:Limit of Detection; D049329:Nanostructures / Q000737:chemistry* / Q000648:ultrastructure; D009570:Nitriles / Q000737:chemistry*; D009584:Nitrogen / Q000737:chemistry; D045663:Quantum Dots / Q000737:chemistry; D015203:Reproducibility of Results; D014025:Titanium / Q000737:chemistry*
chemical_list: D004279:DNA, Viral; D009570:Nitriles; C009495:titanium dioxide; C011206:cyanogen; D006108:Graphite; D014025:Titanium; D009584:Nitrogen
keywords: Photoelectrochemistry; TiO(2) NPs/N-GQDs/g-C(3)N(4) QDs; pcDNA3-HBV