title: Validation of18F-FDG-PET Single-Subject Optimized SPM Procedure with Different PET Scanners.
abstract: 18F-fluoro-deoxy-glucose Positron Emission Tomography (FDG-PET) allows early identification of neurodegeneration in dementia. The use of an optimized method based on the SPM software package highly improves diagnostic accuracy. However, the impact of different scanners for data acquisition on the SPM results and the effects of different pools of healthy subjects on the statistical comparison have not been investigated yet. Images from 144 AD patients acquired using six different PET scanners were analysed with an optimized single-subject SPM procedure to identify the typical AD hypometabolism pattern at single subject level. We compared between-scanners differences on the SPM outcomes in a factorial design. Single-subject SPM comparison analyses were also performed against a different group of healthy controls from the ADNI initiative. The concordance between the two analyses (112 vs. 157 control subjects) was tested using Dice scores. In addition, we applied the optimized single-subject SPM procedure to the FDG-PET data acquired with 3 different scanners in 57 MCI subjects, in order to assess for tomograph influence in early disease phase. All the patients showed comparable AD-like hypometabolic patterns, also in the prodromal phase, in spite of being acquired with different PET scanners. SPM statistical comparisons performed with the two different healthy control databases showed a high degree of concordance (76% average pattern volume overlap and 90% voxel-wise agreement in AD-related brain structures). The validated optimized SPM-based single-subject procedure is influenced neither by the scanners used for image acquisition, nor by differences in healthy control groups, thus implying a great reliability of this method for longitudinal and multicentre studies.
authors.affiliation: Division of Neuroscience, In vivo human molecular and structural neuroimaging unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.|Vita-Salute San Raffaele University, Via Olgettina 60, 20132, Milan, Italy.
authors.forename: Luca
authors.initials: L
authors.lastname: Presotto
authors.affiliation: Division of Neuroscience, In vivo human molecular and structural neuroimaging unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.
authors.forename: Tommaso
authors.initials: T
authors.lastname: Ballarini
authors.affiliation: Division of Neuroscience, In vivo human molecular and structural neuroimaging unit, IRCCS San Raffaele Scientific Institute, Milan, Italy.|Vita-Salute San Raffaele University, Via Olgettina 60, 20132, Milan, Italy.
authors.forename: Silvia Paola
authors.initials: SP
authors.lastname: Caminiti
authors.affiliation: Nuclear Medicine Unit, IRCCS Ospedale San Raffaele, Milan, Italy.
authors.forename: Valentino
authors.initials: V
authors.lastname: Bettinardi
authors.affiliation: Nuclear Medicine Unit, IRCCS Ospedale San Raffaele, Milan, Italy.
authors.forename: Luigi
authors.initials: L
authors.lastname: Gianolli
authors.affiliation: Division of Neuroscience, In vivo human molecular and structural neuroimaging unit, IRCCS San Raffaele Scientific Institute, Milan, Italy. perani.daniela@hsr.it.|Vita-Salute San Raffaele University, Via Olgettina 60, 20132, Milan, Italy. perani.daniela@hsr.it.|Nuclear Medicine Unit, IRCCS Ospedale San Raffaele, Milan, Italy. perani.daniela@hsr.it.
authors.forename: Daniela
authors.initials: D
authors.lastname: Perani
mesh_terms: D000368:Aged; D000369:Aged, 80 and over; D000544:Alzheimer Disease / Q000000981:diagnostic imaging* / Q000473:pathology; D001921:Brain / Q000000981:diagnostic imaging*; D016208:Databases, Factual; D042241:Early Diagnosis; D005260:Female; D019788:Fluorodeoxyglucose F18 / Q000378:metabolism*; D006801:Humans; D008279:Magnetic Resonance Imaging; D008297:Male; D049268:Positron-Emission Tomography / Q000379:methods*; D055818:Public-Private Sector Partnerships; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity
chemical_list: D019788:Fluorodeoxyglucose F18
keywords: 18F–fluoro-deoxy-glucose-PET; Biomarker; Dementia; Early diagnosis; Semi-quantitative method; Single-subject; Statistical method