title: Measuring abscission spatiotemporal dynamics using quantitative high-resolution microscopy.
abstract: The spatiotemporal characteristics of ESCRT (Endosomal Sorting Complex Required for Transport)-mediated mammalian cytokinetic abscission have been studied in recent years using quantitative high-resolution light microscopy techniques. Here we describe how to apply spinning disk live cell imaging and structured illumination microscopy (SIM) to define the dynamics and structural organization of abscission and of proteins involved in abscission in a quantitative manner. We further provide a protocol to correlate the structural data, obtained by SIM, to the dynamic information obtained by live cell recordings.
authors.affiliation: Ben Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: O
authors.initials: O
authors.lastname: Gershony
authors.affiliation: Ben Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Sherman
authors.affiliation: Ben Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: S
authors.initials: S
authors.lastname: Adar
authors.affiliation: Ben Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Segal
authors.affiliation: Ben Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: D
authors.initials: D
authors.lastname: Nachmias
authors.affiliation: Ben Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: I
authors.initials: I
authors.lastname: Goliand
authors.affiliation: Ben Gurion University of the Negev, Beer Sheva, Israel.
authors.forename: N
authors.initials: N
authors.lastname: Elia
mesh_terms: D048749:Cytokinesis / Q000235:genetics*; D056827:Endosomal Sorting Complexes Required for Transport / Q000235:genetics / Q000648:ultrastructure; D011992:Endosomes / Q000235:genetics / Q000648:ultrastructure*; D006367:HeLa Cells; D006801:Humans; D008853:Microscopy / Q000379:methods*; D057054:Molecular Imaging / Q000379:methods*
chemical_list: D056827:Endosomal Sorting Complexes Required for Transport
keywords: Cell cycle; Cell division; Cytokinesis; ESCRT; Intercellular bridge; Live cell imaging; Membrane fission; Structured illumination microscopy