title: Tunable Adsorption and Film Formation of Mussel Adhesive Protein by Potential Control.
abstract: Mussel adhesive proteins are of great interest in many applications because of their outstanding adhesive property and film-forming ability. Understanding and controlling the film formation and its performance is crucial for the effective use of such proteins. In this study, we focus on the potential controlled film formation and compaction of one mussel adhesive protein, Mefp-1. The adsorption and film-forming behavior of Mefp-1 on a platinum (Pt) substrate under applied potentials were investigated by cyclic voltammetry, potential-controlled electrochemical impedance spectroscopy (EIS), and quartz crystal microbalance with dissipation monitoring (QCM-D). Moreover, microfriction measurements were performed to evaluate the mechanical properties of the Mefp-1 films formed at selected potentials. The results led to the conclusion that Mefp-1 adsorbs on the Pt substrate through both electrostatic and nonelectrostatic interactions and shows an effective blocking effect for the electroactive sites on the substrate. The properties of the adsorbed Mefp-1 film vary with the applied potential, and the compactness of the adsorbed Mefp-1 film can be reversibly tuned by the applied potential.
authors.affiliation: Division of Surface and Corrosion Science, Department of Chemistry, School of Chemical Science and Engineering, KTH Royal Institute of Technology , Drottning Kristinas väg 51, SE-100 44 Stockholm, Sweden.
authors.forename: Fan
authors.identifier: 0000-0001-5180-9895
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Division of Surface and Corrosion Science, Department of Chemistry, School of Chemical Science and Engineering, KTH Royal Institute of Technology , Drottning Kristinas väg 51, SE-100 44 Stockholm, Sweden.|State Key Laboratory of Tribology, Tsinghua University , Beijing 100084, China.
authors.forename: Guoxin
authors.initials: G
authors.lastname: Xie
authors.affiliation: Division of Surface and Corrosion Science, Department of Chemistry, School of Chemical Science and Engineering, KTH Royal Institute of Technology , Drottning Kristinas väg 51, SE-100 44 Stockholm, Sweden.
authors.forename: Jinshan
authors.initials: J
authors.lastname: Pan
mesh_terms: D000327:Adsorption; D011506:Proteins / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D011506:Proteins; C037403:adhesive protein, mussel