title: Development and characterization of polymer lipid hybrid nanoparticles for oral delivery of LMWH.
abstract: The present study aimed to develop an improved oral delivery system for low-molecular-weight heparin (LMWH), novel polymer lipid hybrid nanoparticles were developed. LMWH loaded chitosan polymer lipid hybrid nanoparticles (LMWH-CS-PLNs) were developed using double emulsification and solvent evaporation method. The performance of developed formulations was evaluated by using in vitro and in vivo behavior, such as drug release studies, in vitro permeation study, in vivo venous thrombolytic study, in vitro uptake studies by using intestinal epithelium resembling Caco-2 cell lines. The new CS-PLNs might provide an effective strategy for oral delivery of LMWH with improved encapsulation efficiency as compared to CS-NPs and SA-LNPs.
authors.affiliation: a Nanomedicine Research Centre, I.S.F. College of Pharmacy , Moga , India.
authors.forename: Supandeep Singh
authors.initials: SS
authors.lastname: Hallan
authors.affiliation: a Nanomedicine Research Centre, I.S.F. College of Pharmacy , Moga , India.
authors.forename: Veerpal
authors.initials: V
authors.lastname: Kaur
authors.affiliation: b Dr. Reddy's Laboratories Limited , Hyderabad , India.
authors.forename: Vikas
authors.initials: V
authors.lastname: Jain
authors.affiliation: a Nanomedicine Research Centre, I.S.F. College of Pharmacy , Moga , India.
authors.forename: Neeraj
authors.initials: N
authors.lastname: Mishra
mesh_terms: D000284:Administration, Oral; D000588:Amines / Q000737:chemistry*; D001692:Biological Transport; D018938:Caco-2 Cells; D048271:Chitosan / Q000737:chemistry*; D004337:Drug Carriers / Q000737:chemistry* / Q000378:metabolism; D006495:Heparin, Low-Molecular-Weight / Q000008:administration & dosage* / Q000737:chemistry* / Q000627:therapeutic use; D006801:Humans; D053758:Nanoparticles / Q000737:chemistry*; D010539:Permeability; D020246:Venous Thrombosis / Q000188:drug therapy
chemical_list: D000588:Amines; D004337:Drug Carriers; D006495:Heparin, Low-Molecular-Weight; D048271:Chitosan; C009317:stearylamine
keywords: Lipid nanoparticles; chitosan nanoparticles; hydrophilic; low molecular weight heparin; polymer lipid hybrid nanoparticles