title: Evaluate the Relationship Between Class 1 Integrons and Drug Resistance Genes in Clinical Isolates ofPseudomonas aeruginosa.
abstract: BACKGROUND
  The prevalence of resistantPseudomonas aeruginosaisolates is increasing and it is considered as one of the major public health concerns in the world. The association between integrons and drug resistance has been proven and evidences suggest that integrons are coding and responsible for dissemination of antibiotic resistance amongP. aeruginosaisolates.
  
  
  OBJECTIVE
  This study is aimed to evaluate the relationship between class 1 integrons and drug resistance genes in clinical isolates ofP. aeruginosafrom burn patients.
  
  
  METHODS
  100 isolates ofP. aeruginosawere collected from burn patients hospitalized in the skin ward of Shahid Motahari hospital and susceptibility testing was performed by disk diffusion method (Kirby-Bauer). Then DNA was extracted and PCR technique was performed for the detection of class 1 integrons and drug resistance genes. Then data was analyzed using SPSS software.
  
  
  RESULTS
  The most effective antibiotic was polymyxin B with sensitivity 100%, and the most resistance was observed to the ciprofloxacin (93%) and amikacin (67%), respectively. The maximum and lowest frequencies of drug resistance genes belonged to the aac (6 ') - 1, VEB-1 with prevalence rate 93% and 10%, respectively. The statistical Chi-square test did not find any significant correlation between class 1 integrons and drug resistance genes (p˃ 0.05).
  
  
  CONCLUSIONS
  Although no significant correlation between class 1 integrons and drug resistance was observed, but the resistance rate to antibiotics tested amongP. aeruginosaisolates was high. So, surveillance, optimization and strict consideration of antimicrobial use and control of infection are necessary.
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Seyed Mohammad Javad
authors.initials: SM
authors.lastname: Hosseini
authors.affiliation: Pharmaceutical Sciences Brnach, Islamic Azad University, Tehran, Iran.
authors.forename: Niloofar Shoaee
authors.initials: NS
authors.lastname: Naeini
authors.affiliation: Antimicrobial Resistance Research Center, Avicenna Research Institute, Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Azad
authors.initials: A
authors.lastname: Khaledi
authors.affiliation: Applied Microbiology Research Center, Microbiology Department, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Seyede Fatemeh
authors.initials: SF
authors.lastname: Daymad
authors.affiliation: Applied Microbiology Research Center, Microbiology Department, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Davoud
authors.initials: D
authors.lastname: Esmaeili
keywords: Burn ward; Integrons; Pseudomonas aeruginosa; Resistance