title: Methicillin-ResistantStaphylococcus aureusBiofilms and Their Influence on Bacterial Adhesion and Cohesion.
abstract: Twenty-five methicillin-resistantStaphylococcus aureus(MRSA) isolates were characterized by staphylococcal protein A gene typing and the ability to form biofilms. The presence of exopolysaccharides, proteins, and extracellular DNA and RNA in biofilms was assessed by a dispersal assay. In addition, cell adhesion to surfaces and cell cohesion were evaluated using the packed-bead method and mechanical disruption, respectively. The predominant genotype wasspatype t127 (22 out of 25 isolates); the majority of isolates were categorized as moderate biofilm producers. Twelve isolates displayed PIA-independent biofilm formation, while the remaining 13 isolates were PIA-dependent. Both groups showed strong dispersal in response to RNase and DNase digestion followed by proteinase K treatment. PIA-dependent biofilms showed variable dispersal after sodium metaperiodate treatment, whereas PIA-independent biofilms showed enhanced biofilm formation. There was no correlation between the extent of biofilm formation or biofilm components and the adhesion or cohesion abilities of the bacteria, but the efficiency of adherence to glass beads increased after biofilm depletion. In conclusion, nucleic acids and proteins formed the main components of the MRSA clone t127 biofilm matrix, and there seems to be an association between adhesion and cohesion in the biofilms tested.
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Khulood Hamid
authors.initials: KH
authors.lastname: Dakheel
authors.affiliation: Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Raha
authors.initials: R
authors.lastname: Abdul Rahim
authors.affiliation: Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Vasantha Kumari
authors.identifier: 0000-0002-4076-8335
authors.initials: VK
authors.lastname: Neela
authors.affiliation: Agro-Biotechnology Institute Malaysia (ABI), c/o MARDI Headquarters, 43400 Serdang, Selangor, Malaysia.
authors.forename: Jameel R
authors.identifier: 0000-0002-5705-0223
authors.initials: JR
authors.lastname: Al-Obaidi
authors.affiliation: Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
authors.forename: Tan Geok
authors.initials: TG
authors.lastname: Hun
authors.affiliation: Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia; Institute of Bioscience, Universiti Putra Malaysia, 43400 Serdang, Selangor Darul Ehsan, Malaysia.
authors.forename: Khatijah
authors.identifier: 0000-0002-7683-4760
authors.initials: K
authors.lastname: Yusoff
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects* / Q000235:genetics; D018441:Biofilms / Q000187:drug effects / Q000254:growth & development*; D006801:Humans; D016106:Methicillin Resistance / Q000187:drug effects; D055624:Methicillin-Resistant Staphylococcus aureus / Q000187:drug effects / Q000235:genetics / Q000254:growth & development*; D010504:Periodic Acid / Q000494:pharmacology; D013203:Staphylococcal Infections / Q000188:drug therapy* / Q000235:genetics / Q000382:microbiology; D013205:Staphylococcal Protein A / Q000235:genetics / Q000378:metabolism
chemical_list: D013205:Staphylococcal Protein A; D010504:Periodic Acid; C009288:metaperiodate