title: Energetic, oxidative and ionic exchange in rat brain and liver mitochondria at experimental audiogenic epilepsy (Krushinsky-Molodkina model).
abstract: The role of brain and liver mitochondria at epileptic seizure was studied on Krushinsky-Molodkina (KM) rats which respond to sound with an intensive epileptic seizure (audiogenic epilepsy). We didn't find significant changes in respiration rats of brain and liver mitochondria of KM and control rats; however the efficiency of АТР synthesis in the KM rat mitochondria was 10% lower. In rats with audiogenic epilepsy the concentration of oxidative stress marker malondialdehyde in mitochondria of the brain (but not liver) was 2-fold higher than that in the control rats. The rate of H2O2generation in brain mitochondria of КМ rats was twofold higher than in the control animals when using NAD-dependent substrates. This difference was less pronounced in liver mitochondria. In KM rats, the activity of mitochondrial ATP-dependent potassium channel was lower than in liver mitochondria of control rats. The comparative study of the mitochondria ability to retain calcium ions revealed that in the case of using the complex I and complex II substrates, permeability transition pore is easier to trigger in brain and liver mitochondria of KM and КМs rats than in the control ones. The role of the changes in the energetic, oxidative, and ionic exchange in the mechanism of audiogenic epilepsy generation in rats and the possible correction of the epilepsy seizures are discussed.
authors.affiliation: Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia. tiggra4@yandex.ru.
authors.forename: Natalya I
authors.initials: NI
authors.lastname: Venediktova
authors.affiliation: Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Olga S
authors.initials: OS
authors.lastname: Gorbacheva
authors.affiliation: Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Natalia V
authors.initials: NV
authors.lastname: Belosludtseva
authors.affiliation: Biology Department, Laboratory for Physiology and Genetics of Behavior, Lomonosov Moscow State University, Leninskie Gory, 1, Build. 12, Moscow, 119992, Russia.
authors.forename: Irina B
authors.initials: IB
authors.lastname: Fedotova
authors.affiliation: Biology Department, Laboratory for Physiology and Genetics of Behavior, Lomonosov Moscow State University, Leninskie Gory, 1, Build. 12, Moscow, 119992, Russia.
authors.forename: Natalia M
authors.initials: NM
authors.lastname: Surina
authors.affiliation: Biology Department, Laboratory for Physiology and Genetics of Behavior, Lomonosov Moscow State University, Leninskie Gory, 1, Build. 12, Moscow, 119992, Russia.
authors.forename: Inga I
authors.initials: II
authors.lastname: Poletaeva
authors.affiliation: Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Oleg V
authors.initials: OV
authors.lastname: Kolomytkin
authors.affiliation: Institute of Theoretical and Experimental Biophysics RAS, Pushchino, Moscow Region, 142290, Russia.
authors.forename: Galina D
authors.initials: GD
authors.lastname: Mironova
mesh_terms: D000255:Adenosine Triphosphate / Q000096:biosynthesis; D000818:Animals; D001921:Brain / Q000378:metabolism*; D002118:Calcium / Q000378:metabolism; D020195:Epilepsy, Reflex / Q000378:metabolism*; D006861:Hydrogen Peroxide / Q000378:metabolism; D008099:Liver / Q000378:metabolism; D008928:Mitochondria / Q000378:metabolism*; D008930:Mitochondria, Liver / Q000378:metabolism*; D033681:Mitochondrial Membrane Transport Proteins / Q000378:metabolism; D000083162:Mitochondrial Permeability Transition Pore; D018384:Oxidative Stress; D011919:Rats, Inbred Strains
chemical_list: D033681:Mitochondrial Membrane Transport Proteins; D000083162:Mitochondrial Permeability Transition Pore; D000255:Adenosine Triphosphate; D006861:Hydrogen Peroxide; D002118:Calcium
keywords: Audiogenic epilepsy; H2O2 production; Ions transport; Respiration; mitoKATP