title: [Clinicopathologic features of mammary analogue secretory carcinoma of salivary glands].
abstract: Objective:To investigate the clinicopathological features of mammary analogue secretory carcinoma (MASC) of salivary glands, and its diagnosis, differential diagnosis, immunohistochemistry and molecular pathology.Methods:Seventeen cases of MASC were enrolled, with 9 cases of salivary acinar cell carcinoma and 18 cases of adenoid cystic carcinoma as control groups from Nanjing General Hospital from 1997 to 2014 were included in this retrospective study, combined with immunohistochemistry and molecular detection of ETV6-NTRK3 gene fusion. All cases were histologically reviewed with immunohistochemical staining (EnVision) for S-100 protein, SOX10, GATA3, CD117 expression in each group. Fluorescence in situ hybridization (FISH) was used to detect the ETV6-NTRK3 gene fusion.Results:The age of MASC patients ranged from 27 to 74 years with mean age of 47 and ratio of male and female was 4∶3. All cases showed infiltrative growth and diverse cytology and histology, including lobular (8 cases), cystic papillary (3 cases), cribriform mixed with papillary and glandular structures (6 cases) at various proportions. Some tumors of MASC also exhibited solid growth areas with occasional microcystic honeycombed pattern composed of small cysts merged into larger cysts resembling thyroid follicles. S-100 protein and SOX10 were strongly positive in all MASC cases (17/17). In addition, there was insignificant positivity for GATA3 (3/17) and CD117 (4/17). ETV6 gene fusion detection was informative in 12 MASC cases by FISH with 10 positive cases and 2 negative cases.Conclusions:Combined immunohistochemical positivity of S-100 protein, CD117 and SOX10 are useful in the diagnosis and differential diagnosis of MASC. FISH detection of ETV6-NTRK3 fusion offers an additional molecular diagnostic marker for the diagnosis.
authors.affiliation: Department of Pathology, Medicine School of Nanjing University/Nanjing Jinling Hospital, Nanjing 210002, China.
authors.forename: X P
authors.initials: XP
authors.lastname: Zhang
authors.forename: H
authors.initials: H
authors.lastname: Ni
authors.forename: X
authors.initials: X
authors.lastname: Wang
authors.forename: H
authors.initials: H
authors.lastname: Chen
authors.forename: S S
authors.initials: SS
authors.lastname: Shi
authors.forename: B
authors.initials: B
authors.lastname: Yu
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhou
authors.forename: Q
authors.initials: Q
authors.lastname: Rao
mesh_terms: D000328:Adult; D000368:Aged; D014408:Biomarkers, Tumor / Q000032:analysis; D018267:Carcinoma, Acinar Cell / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D003937:Diagnosis, Differential; D005260:Female; D050990:GATA3 Transcription Factor / Q000032:analysis; D050939:Gene Fusion; D006801:Humans; D007150:Immunohistochemistry; D017404:In Situ Hybridization, Fluorescence; D008297:Male; D000069295:Mammary Analogue Secretory Carcinoma / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D008875:Middle Aged; D015514:Oncogene Proteins, Fusion; D050783:Proto-Oncogene Proteins c-ets / Q000235:genetics; D019009:Proto-Oncogene Proteins c-kit / Q000032:analysis; D020812:Receptor, trkC / Q000235:genetics; D012097:Repressor Proteins / Q000235:genetics; D012189:Retrospective Studies; D009418:S100 Proteins / Q000032:analysis; D055757:SOXE Transcription Factors / Q000032:analysis; D012468:Salivary Gland Neoplasms / Q000175:diagnosis / Q000235:genetics / Q000473:pathology*; D012469:Salivary Glands; D000097764:ETS Translocation Variant 6 Protein
chemical_list: D014408:Biomarkers, Tumor; D050990:GATA3 Transcription Factor; D015514:Oncogene Proteins, Fusion; D050783:Proto-Oncogene Proteins c-ets; D019009:Proto-Oncogene Proteins c-kit; D020812:Receptor, trkC; D012097:Repressor Proteins; D009418:S100 Proteins; D055757:SOXE Transcription Factors; D000097764:ETS Translocation Variant 6 Protein; C494749:GATA3 protein, human; C527923:SOX10 protein, human