title: Transcriptional regulation of follicle-stimulating hormone β-subunit in marmoset by an alternate distal promoter.
abstract: Follicle-stimulating hormone (FSH) is essential for mammalian folliculogenesis and spermatogenesis. Common marmoset (Callithrix jacchus) is a New World primate which exhibits an unusual FSH profile across the ovarian cycle with a mid-follicular FSH peak that is not observed in Catarrhini primates like humans. Since transcription of FSH β-subunit gene (FSHβ) is a rate-limiting step in the production of mature FSH, this study aimed to investigate the regulation of marmoset FSHβ gene expression in comparison to human. In silico analysis of the FSHβ promoter sequences identified a TATA box element upstream of the conventional TATA box element in marmoset but not in human sequence. FSHβ mRNA transcript longer than the conventional transcript was detected in marmoset pituitary implying presence of a distal transcription start site. In luciferase reporter assays, the marmoset putative distal promoter had higher activity than the corresponding human region even in absence of the conventional proximal promoter. Indeed higher affinity binding of TATA box-binding protein to the putative distal TATA box element was obtained in electrophoretic mobility shift assay. This suggests existence of a differential regulation of FSHβ transcription in marmoset compared to humans.
authors.affiliation: Division of Structural Biology, National Institute for Research in Reproductive Health (Indian Council of Medical Research), Jehangir Merwanji Street, Parel, Mumbai 400012, India.
authors.forename: Susha S
authors.initials: SS
authors.lastname: Kutteyil
authors.affiliation: Division of Structural Biology, National Institute for Research in Reproductive Health (Indian Council of Medical Research), Jehangir Merwanji Street, Parel, Mumbai 400012, India. Electronic address: pathakb@nirrh.res.in.
authors.forename: Bhakti R
authors.initials: BR
authors.lastname: Pathak
authors.affiliation: Division of Structural Biology, National Institute for Research in Reproductive Health (Indian Council of Medical Research), Jehangir Merwanji Street, Parel, Mumbai 400012, India. Electronic address: smitamahale@hotmail.com.
authors.forename: Smita D
authors.initials: SD
authors.lastname: Mahale
mesh_terms: D000818:Animals; D002144:Callithrix / Q000378:metabolism*; D005260:Female; D005640:Follicle Stimulating Hormone / Q000378:metabolism*; D006801:Humans; D008992:Monkey Diseases / Q000378:metabolism*; D015533:Transcriptional Activation
chemical_list: D005640:Follicle Stimulating Hormone
keywords: Follicle-stimulating hormone; Marmoset; TATA box; Transcription; β-Subunit