title: Murine strain differences in inflammatory angiogenesis of internal wound in diabetes.
abstract: Genetic susceptibility is associated with inflammation, neovascularization, and diabetes phenotypes. However, to what extent this susceptibility influences inflammatory angiogenesis in internal injuries in diabetes has not been fully investigated. Using the subcutaneous implantation of a synthetic matrix as an internal wound model in Swiss, C57BL/6 and Balb/c mice, we have studied inflammation, angiogenesis, and cytokine production in the fibrovascular tissue induced by implants in diabetic animals. The hyperglycemic levels (mg/dl) after the diabetogenic treatment were 455.0±15 in Swiss, 393.0±22 in C57BL/6, and 190.0±10 in Balb/c mice. Angiogenesis in Swiss implants from non-diabetic animals were higher than those in the implants from the other strains. However, the angiogenic inducers VEGF and nitric oxide (NO) were higher in implants from non-diabetic Swiss and Balb/c mice. Strain-related differences were also observed in the angiogenic parameters in implants from diabetic mice. Hb content and number of vessels decreased more than 40% in Swiss implants. In contrast, Hb content did not alter in implants from Balb/c diabetic mice and the number of vessels decreased. VEGF levels increased in implants from Swiss and C57BL/6 diabetic mice, but decreased in Balb/c implants. The levels of pro-inflammatory markers intra-implant also varied among the strains in both conditions. In the hyperglycemic environment, almost all inflammatory markers increased in implants from diabetic Swiss mice. These findings demonstrate the major contribution of genetic background in the pattern of inflammatory angiogenesis components of internal injury, in both normoglycemic and hyperglycemic animals.
authors.affiliation: Department of General Pathology, Federal University of Minas Gerais, Institute of Biological Sciences, Av. Antônio Carlos 6627 - Campus Pampulha, Cx Post 468, CEP 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Simone Aparecida de
authors.initials: SA
authors.lastname: Almeida
authors.affiliation: Department of General Pathology, Federal University of Minas Gerais, Institute of Biological Sciences, Av. Antônio Carlos 6627 - Campus Pampulha, Cx Post 468, CEP 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Laura Alejandra Ariza
authors.initials: LA
authors.lastname: Orellano
authors.affiliation: Department of General Pathology, Federal University of Minas Gerais, Institute of Biological Sciences, Av. Antônio Carlos 6627 - Campus Pampulha, Cx Post 468, CEP 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Luciana Xavier
authors.initials: LX
authors.lastname: Pereira
authors.affiliation: Department of General Pathology, Federal University of Minas Gerais, Institute of Biological Sciences, Av. Antônio Carlos 6627 - Campus Pampulha, Cx Post 468, CEP 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Celso Tarso Rodrigues
authors.initials: CT
authors.lastname: Viana
authors.affiliation: Department of General Pathology, Federal University of Minas Gerais, Institute of Biological Sciences, Av. Antônio Carlos 6627 - Campus Pampulha, Cx Post 468, CEP 31270-901, Belo Horizonte, MG, Brazil. Electronic address: paulapc@icb.ufmg.br.
authors.forename: Paula Peixoto
authors.initials: PP
authors.lastname: Campos
authors.affiliation: Department of Physiology and Biophysics Institute of Biological Sciences, Federal University of Minas Gerais, Av. Antônio Carlos 6627 - Campus Pampulha, Cx Post 468, CEP 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Silvia Passos
authors.initials: SP
authors.lastname: Andrade
authors.affiliation: Department of General Pathology, Federal University of Minas Gerais, Institute of Biological Sciences, Av. Antônio Carlos 6627 - Campus Pampulha, Cx Post 468, CEP 31270-901, Belo Horizonte, MG, Brazil.
authors.forename: Monica Alves Neves Diniz
authors.initials: MA
authors.lastname: Ferreira
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D003921:Diabetes Mellitus, Experimental / Q000378:metabolism / Q000473:pathology*; D007249:Inflammation / Q000378:metabolism / Q000473:pathology*; D051379:Mice; D008807:Mice, Inbred BALB C; D008810:Mice, Inbred C57BL; D009389:Neovascularization, Pathologic / Q000378:metabolism / Q000473:pathology*; D009569:Nitric Oxide / Q000378:metabolism; D042461:Vascular Endothelial Growth Factor A / Q000378:metabolism; D014945:Wound Healing / Q000502:physiology; D014947:Wounds and Injuries / Q000378:metabolism / Q000473:pathology*
chemical_list: D015415:Biomarkers; D042461:Vascular Endothelial Growth Factor A; D009569:Nitric Oxide
keywords: Angiogenesis; Diabetes; Genetic background; Internal wound