title: Evidence That Trimethyllysine Hydroxylase Catalyzes the Formation of (2S,3S)-3-Hydroxy-Nε-trimethyllysine.
abstract: Trimethyllysine hydroxylase (TMLH) is an Fe(II) and 2-oxoglutarate (2OG) dependent oxygenase involved in the biomedically important carnitine biosynthesis pathway. A combination of synthetic and NMR studies provides direct evidence that human TMLH catalyzes the stereoselective conversion of (2S)-Nε-trimethyllysine to (2S,3S)-3-hydroxy-Nε-trimethyllysine.
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Y Vijayendar
authors.initials: YV
authors.lastname: Reddy
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Abbas H K
authors.initials: AH
authors.lastname: Al Temimi
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Paul B
authors.initials: PB
authors.lastname: White
authors.affiliation: Institute for Molecules and Materials, Radboud University , Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands.
authors.forename: Jasmin
authors.identifier: 0000-0002-5559-3822
authors.initials: J
authors.lastname: Mecinović
mesh_terms: D055162:Biocatalysis; D002331:Carnitine; D006801:Humans; D008239:Lysine / Q000031:analogs & derivatives; D006899:Mixed Function Oxygenases / Q000378:metabolism*; D015394:Molecular Structure
chemical_list: C003712:trimethyllysine; D006899:Mixed Function Oxygenases; C025931:trimethyl-lysine hydroxylase; D008239:Lysine; D002331:Carnitine