title: Human heart valve-derived scaffold improves cardiac repair in a murine model of myocardial infarction.
abstract: Cardiac tissue engineering using biomaterials with or without combination of stem cell therapy offers a new option for repairing infarcted heart. However, the bioactivity of biomaterials remains to be optimized because currently available biomaterials do not mimic the biochemical components as well as the structural properties of native myocardial extracellular matrix. Here we hypothesized that human heart valve-derived scaffold (hHVS), as a clinically relevant novel biomaterial, may provide the proper microenvironment of native myocardial extracellular matrix for cardiac repair. In this study, human heart valve tissue was sliced into 100 μm tissue sheet by frozen-sectioning and then decellularized to form the hHVS. Upon anchoring onto the hHVS, post-infarct murine BM c-kit+ cells exhibited an increased capacity for proliferation and cardiomyogenic differentiation in vitro. When used to patch infarcted heart in a murine model of myocardial infarction, either implantation of the hHVS alone or c-kit+ cell-seeded hHVS significantly improved cardiac function and reduced infarct size; while c-kit+ cell-seeded hHVS was even superior to the hHVS alone. Thus, we have successfully developed a hHVS for cardiac repair. Our in vitro and in vivo observations provide the first clinically relevant evidence for translating the hHVS-based biomaterials into clinical strategies to treat myocardial infarction.
authors.affiliation: Laboratory of Cardiovascular Sciences, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Long
authors.initials: L
authors.lastname: Wan
authors.affiliation: Laboratory of Cardiovascular Sciences, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Zhenhua
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Weijun
authors.initials: W
authors.lastname: Wang
authors.affiliation: Laboratory of Cardiovascular Sciences, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Sebastian
authors.initials: S
authors.lastname: Schmull
authors.affiliation: German Rheumatism Research Centre, Berlin, Germany.
authors.forename: Jun
authors.initials: J
authors.lastname: Dong
authors.affiliation: Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Song
authors.initials: S
authors.lastname: Xue
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland.
authors.forename: Hans
authors.initials: H
authors.lastname: Imboden
authors.affiliation: Laboratory of Cardiovascular Sciences, Renji-Med X Clinical Stem Cell Research Center, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.|Department of Cardiovascular Surgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Li
mesh_terms: D000818:Animals; D001672:Biocompatible Materials; D001854:Bone Marrow Cells / Q000502:physiology* / Q000648:ultrastructure; D002454:Cell Differentiation; D049109:Cell Proliferation; D002478:Cells, Cultured; D004195:Disease Models, Animal; D006801:Humans; D008297:Male; D008810:Mice, Inbred C57BL; D008943:Mitral Valve / Q000502:physiology* / Q000648:ultrastructure; D009203:Myocardial Infarction / Q000628:therapy*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds
chemical_list: D001672:Biocompatible Materials