title: Liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) based bioavailability determination of the major classes of phytochemicals.
abstract: Natural products derived from plants have served as an inexhausted source for drug discovery and drug development. They have been evolutionary amplified with drug-like properties and have already illustrated immense therapeutic potential over an array of different diseases. However, their incorporation in the drug discovery pipeline has been diminished the last two decades. This was probably due to barriers related to their inherent difficulties to be integrated in high-throughput screening assays as also their largely unexplored bioavailability. Analytical procedures have come into the spotlight, a result of the continuous development of the instrumentation's capabilities as far as detection and separation is concerned. Integral part of this technological evolution is LC-MS instrumentation and its extended use for the determination of various compounds. The fact that it provides extra sensitivity, specificity and good separation in complex samples, makes LC-MS/MS the ultimate tool in the determination of many types of chemical compounds, such as phytochemicals. Herein, we focus on the achievements of the last five years in quantitative analysis of the major classes of phytochemicals (flavonoids, alkaloids, terpenes, glycosides and saponins) in plasma, through LC-MS/MS, as also their bioavailability.
authors.affiliation: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece; Biotechnology Laboratory, Department of Biological Applications and Technologies, University of Ioannina, Ioannina, 45110, Greece.
authors.forename: Evgenios
authors.initials: E
authors.lastname: Stylos
authors.affiliation: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece.
authors.forename: Maria V
authors.initials: MV
authors.lastname: Chatziathanasiadou
authors.affiliation: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece.
authors.forename: Aggeliki
authors.initials: A
authors.lastname: Syriopoulou
authors.affiliation: Section of Organic Chemistry and Biochemistry, Department of Chemistry, University of Ioannina, Ioannina, 45110, Greece. Electronic address: agtzakos@gmail.com.
authors.forename: Andreas G
authors.initials: AG
authors.lastname: Tzakos
mesh_terms: D000470:Alkaloids / Q000032:analysis / Q000493:pharmacokinetics; D000818:Animals; D001682:Biological Availability; D002853:Chromatography, Liquid / Q000379:methods*; D005419:Flavonoids / Q000032:analysis / Q000493:pharmacokinetics; D006027:Glycosides / Q000032:analysis / Q000493:pharmacokinetics; D006801:Humans; D064209:Phytochemicals / Q000032:analysis / Q000493:pharmacokinetics*; D012503:Saponins / Q000032:analysis / Q000493:pharmacokinetics; D053719:Tandem Mass Spectrometry / Q000379:methods*; D013729:Terpenes / Q000032:analysis / Q000493:pharmacokinetics
chemical_list: D000470:Alkaloids; D005419:Flavonoids; D006027:Glycosides; D064209:Phytochemicals; D012503:Saponins; D013729:Terpenes
keywords: Bioavailability; Liquid chromatography; Phytochemicals; Plasma; Triple quadrupole