title: Immunolocalization in Secondary Xylem of Populus.
abstract: Immunolocalization can be used to precisely visualize the location of specific proteins, cell wall components, or any other molecules within cells or tissues for which specific antibodies are available. Here we describe an immunolocalization protocol for tissue sections of woody Populus stems. The protocol includes descriptions of the required sectioning, fixation, probing, detection, and imaging parameters, as well suggested controls useful in interpreting results.
authors.affiliation: Forest Service, Pacific Southwest Research Station, 1738 Research Park Dr, Davis, CA, USA.
authors.forename: Suzanne
authors.initials: S
authors.lastname: Gerttula
authors.affiliation: Forest Service, Pacific Southwest Research Station, 1738 Research Park Dr, Davis, CA, USA. agroover@fs.fed.us.|Department of Plant Biology, Life Sciences Addition, University of California, Davis, CA, USA. agroover@fs.fed.us.
authors.forename: Andrew
authors.initials: A
authors.lastname: Groover
mesh_terms: D015415:Biomarkers; D005455:Fluorescent Antibody Technique; D018613:Microscopy, Confocal; D057054:Molecular Imaging; D010940:Plant Proteins / Q000378:metabolism; D032107:Populus / Q000166:cytology* / Q000378:metabolism*; D021381:Protein Transport; D014934:Wood; D052584:Xylem / Q000166:cytology* / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D010940:Plant Proteins
keywords: Antibodies; Antigen; Cell wall; Forest trees; Immunolocalization; Wood development