title: Prevalence of Chlamydophila pneumoniae and Mycoplasma pneumoniae IgM and IgG antibodies in Tunisian patients presenting with exacerbation of chronic obstructive pulmonary disease.
abstract: OBJECTIVE
  We aimed to assess the prevalence of Chlamydophila pneumoniae and Mycoplasma pneumoniae acute infections, using serological testing, in patients admitted to the emergency department for acute exacerbations of chronic obstructive pulmonary disease (COPD).
  
  
  METHODS
  We performed a prospective observational study of 100 consecutive patients. Serum specimens were collected at day 0 and day 15. C. pneumoniae and M. pneumoniae antibodies (IgM and IgG) were tested by commercial ELISA and immunofluorescence assay, respectively.
  
  
  RESULTS
  We did not observe any acute M. pneumoniae infection; however, 11 patients (11%) showed a profile compatible with a recent C. pneumoniae infection (nine patients with specific IgM and two with an IgG antibody rise). Demographic and clinical parameters did not differ between patients with and without biological profile of recent C. pneumoniae infection.
  
  
  CONCLUSIONS
  C. pneumoniae is a pathogen that requires specific antimicrobial treatment. Its detection must always be performed considering its prevalence in patients presenting with acute COPD exacerbations.
authors.affiliation: Research laboratory (LR12SP18), university of Monastir, Monastir, Tunisia; Microbiology laboratory, Fattouma Bourguiba university hospital of Monastir, Monastir, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Messous
authors.affiliation: Research laboratory (LR12SP18), university of Monastir, Monastir, Tunisia.
authors.forename: I
authors.initials: I
authors.lastname: Trabelsi
authors.affiliation: Research laboratory (LR12SP18), university of Monastir, Monastir, Tunisia; Department of emergency, Fattouma Bourguiba university hospital of Monastir, Monastir, Tunisia.
authors.forename: M H
authors.initials: MH
authors.lastname: Grissa
authors.affiliation: Research laboratory (LR12SP18), university of Monastir, Monastir, Tunisia; Department of emergency, Fattouma Bourguiba university hospital of Monastir, Monastir, Tunisia.
authors.forename: S
authors.initials: S
authors.lastname: Nouira
authors.affiliation: Laboratory of infectious agents and hygiene, university hospital of Saint-Étienne, Hôpital Nord, 42055 Saint-Étienne cedex 02, France. Electronic address: bruno.pozzetto@univ-st-etienne.fr.
authors.forename: B
authors.initials: B
authors.lastname: Pozzetto
authors.affiliation: Microbiology laboratory, Fattouma Bourguiba university hospital of Monastir, Monastir, Tunisia.
authors.forename: M
authors.initials: M
authors.lastname: Mastouri
mesh_terms: D000368:Aged; D000907:Antibodies, Bacterial / Q000097:blood*; D023521:Chlamydophila Infections / Q000097:blood* / Q000150:complications / Q000453:epidemiology*; D016993:Chlamydophila pneumoniae / Q000276:immunology*; D018450:Disease Progression; D005260:Female; D006801:Humans; D007074:Immunoglobulin G / Q000097:blood*; D007075:Immunoglobulin M / Q000097:blood*; D008297:Male; D008875:Middle Aged; D009177:Mycoplasma pneumoniae / Q000276:immunology*; D018410:Pneumonia, Bacterial / Q000097:blood* / Q000150:complications / Q000453:epidemiology*; D011019:Pneumonia, Mycoplasma / Q000097:blood* / Q000150:complications / Q000453:epidemiology*; D015995:Prevalence; D011446:Prospective Studies; D029424:Pulmonary Disease, Chronic Obstructive / Q000097:blood* / Q000150:complications*; D014416:Tunisia
chemical_list: D000907:Antibodies, Bacterial; D007074:Immunoglobulin G; D007075:Immunoglobulin M
keywords: Bronchopneumopathie chronique obstructive; Chlamydophila pneumoniae; Chronic obstructive pulmonary disease; Mycoplasma pneumoniae