title: MiR-142 modulates human pancreatic cancer proliferation and invasion by targeting hypoxia-inducible factor 1 (HIF-1α) in the tumor microenvironments.
abstract: MicroRNAs regulate most protein-coding genes, including genes important in cancer and other diseases. In this study, we demonstrated that the expression of miR-142 could be significantly suppressed in pancreatic cancer specimens and cell lines compared to their adjacent tissues and normal pancreatic cells. Growth and invasion of PANC-1 and SW1990 cells were attenuated by overexpression of miR-142in vitroWith the help of bioinformatics analysis, hypoxia-inducible factor 1 (HIF-1α) was identified to be a direct target of miR-142, and a luciferase reporter experiment confirmed this discovery. Overexpression of miR-142 decreases protein expression of HIF-1α. In the hypoxic microenvironment, HIF-1α was up-regulated while miR-142 was down-regulated. The invaded cells significantly increased in the hypoxic microenvironment compared to the normoxic microenvironment. The hypoxia treatment induced cells' proliferation, and invasion could be inhibited by miR-142 overexpression or HIF-1α inhibition. Moreover, expression of epithelial-mesenchymal transition (EMT) markers, Vimentin, VEGF-C and E-cad, was altered under hypoxia conditions and regulated by miR-142/HIF-1α. Above all, these findings provided insights on the functional mechanism of miR-142, suggesting that the miR-142/HIF-1α axis may interfere with the proliferative and invasive properties of pancreatic cancer cells, and indicated that miR-142 could be a potential therapeutic target for pancreatic cancer.
authors.affiliation: Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: Yebin
authors.initials: Y
authors.lastname: Lu
authors.affiliation: Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: Niandong
authors.initials: N
authors.lastname: Ji
authors.affiliation: Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, People's Republic of China xy_weiwei@163.com.
authors.forename: Wei
authors.identifier: http://orcid.org/0000-0002-4027-9152
authors.initials: W
authors.lastname: Wei
authors.affiliation: Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: Weijia
authors.initials: W
authors.lastname: Sun
authors.affiliation: Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: Xuejun
authors.initials: X
authors.lastname: Gong
authors.affiliation: Department of Gerneral Surgery, Xiangya Hospital, Central South University, Changsha, Hunan Province, People's Republic of China.
authors.forename: Xitao
authors.initials: X
authors.lastname: Wang
keywords: Cell invasion; Cell proliferation; HIF-1α; Hypoxia microenvironment; MiR-142; Pancreatic cancer