title: Wolbachia Infections in Aedes aegypti Differ Markedly in Their Response to Cyclical Heat Stress.
abstract: Aedes aegypti mosquitoes infected with Wolbachia bacteria are currently being released for arbovirus suppression around the world. Their potential to invade populations and persist will depend on interactions with environmental conditions, particularly as larvae are often exposed to fluctuating and extreme temperatures in the field. We reared Ae. aegypti larvae infected with different types of Wolbachia (wMel, wAlbB and wMelPop-CLA) under diurnal cyclical temperatures. Rearing wMel and wMelPop-CLA-infected larvae at 26-37°C reduced the expression of cytoplasmic incompatibility, a reproductive manipulation induced by Wolbachia. We also observed a sharp reduction in the density of Wolbachia in adults. Furthermore, the wMel and wMelPop-CLA infections were not transmitted to the next generation when mosquitoes were exposed to 26-37°C across all life stages. In contrast, the wAlbB infection was maintained at a high density, exhibited complete cytoplasmic incompatibility, and was transmitted from mother to offspring with a high fidelity under this temperature cycle. These findings have implications for the potential success of Wolbachia interventions across different environments and highlight the importance of temperature control in rearing.
authors.affiliation: Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Perran A
authors.identifier: 0000-0001-7645-7523
authors.initials: PA
authors.lastname: Ross
authors.affiliation: Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.|Department of Plant Production Technology, Faculty of Agricultural Technology, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand.
authors.forename: Itsanun
authors.initials: I
authors.lastname: Wiwatanaratanabutr
authors.affiliation: Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Jason K
authors.identifier: 0000-0003-4788-3691
authors.initials: JK
authors.lastname: Axford
authors.affiliation: Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Vanessa L
authors.initials: VL
authors.lastname: White
authors.affiliation: Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Nancy M
authors.identifier: 0000-0002-9834-4068
authors.initials: NM
authors.lastname: Endersby-Harshman
authors.affiliation: Pest and Environmental Adaptation Research Group, Bio21 Institute and the School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
authors.forename: Ary A
authors.initials: AA
authors.lastname: Hoffmann
mesh_terms: D000330:Aedes / Q000382:microbiology*; D000818:Animals; D004777:Environment; D006358:Hot Temperature; D007303:Insect Vectors / Q000382:microbiology; D010572:Pest Control, Biological / Q000379:methods*; D020577:Wolbachia / Q000254:growth & development*