title: On-chip wavelength multiplexed detection of cancer DNA biomarkers in blood.
abstract: We have developed an optofluidic analysis system that processes biomolecular samples starting from whole blood and then analyzes and identifies multiple targets on a silicon-based molecular detection platform. We demonstrate blood filtration, sample extraction, target enrichment, and fluorescent labeling using programmable microfluidic circuits. We detect and identify multiple targets using a spectral multiplexing technique based on wavelength-dependent multi-spot excitation on an antiresonant reflecting optical waveguide chip. Specifically, we extract two types of melanoma biomarkers, mutated cell-free nucleic acids -BRAFV600E and NRAS, from whole blood. We detect and identify these two targets simultaneously using the spectral multiplexing approach with up to a 96% success rate. These results point the way toward a full front-to-back chip-based optofluidic compact system for high-performance analysis of complex biological samples.
authors.affiliation: School of Engineering, University of California , Santa Cruz. 1156 High Street, Santa Cruz, California 95064, USA.
authors.forename: H
authors.initials: H
authors.lastname: Cai
authors.affiliation: Department of Electrical and Computer Engineering, Brigham Young University , 459 Clyde Building, Provo, Utah 84602, USA.
authors.forename: M A
authors.initials: MA
authors.lastname: Stott
authors.affiliation: School of Engineering, University of California , Santa Cruz. 1156 High Street, Santa Cruz, California 95064, USA.
authors.forename: D
authors.initials: D
authors.lastname: Ozcelik
authors.affiliation: School of Engineering, University of California , Santa Cruz. 1156 High Street, Santa Cruz, California 95064, USA.
authors.forename: J W
authors.initials: JW
authors.lastname: Parks
authors.affiliation: Department of Electrical and Computer Engineering, Brigham Young University , 459 Clyde Building, Provo, Utah 84602, USA.
authors.forename: A R
authors.initials: AR
authors.lastname: Hawkins
authors.affiliation: School of Engineering, University of California , Santa Cruz. 1156 High Street, Santa Cruz, California 95064, USA.
authors.forename: H
authors.initials: H
authors.lastname: Schmidt