title: Analysis of 12 X-STR loci in the population of south Croatia.
abstract: The aim of the study was to assess forensic pertinence of 12 short tandem repeats (STRs) on X-chromosome in south Croatia population. Investigator®Argus X-12 kit was used to co-amplify 12 STR loci belonging to four linkage groups (LGs) on X-chromosome in 99 male and 98 female DNA samples of unrelated donors. PCR products were analyzed by capillary electrophoresis. Population genetic and forensic parameters were calculated by the Arlequin and POPTREE2 software, and an on-line tool available at ChrX-STR.org. Hardy-Weinberg equilibrium was confirmed for all X-STR markers in female samples. Biallelic patterns at DXS10079 locus were detected in four male samples. Polymorphism information content for the most (DXS10135) and the least (DXS8378) informative markers was 0.9212 and 0.6347, respectively. In both male and female samples, combined power of discrimination exceeded 0.999999999. As confirmed by linkage disequilibrium test, significant association of marker pair DXS10074-DXS10079 (P = 0.0004) within LG2 and marker pair DXS10101-DXS10103 (P = 0.0003) within LG3 was found only in male samples. Number of observed haplotypes in our sample pool amounted 3.01, 7.53, 5 and 3.25% of the number of possible haplotypes for LG1, LG2, LG3 and LG4, respectively. According to haplotype diversity value of 0.9981, LG1 was the most informative. In comparison of south Croatia with 26 world populations, pair-wise [Formula: see text] values increase in parallel with geographical distance. Overall statistical assessment confirmed suitability of Investigator®Argus X-12 kit for forensic casework in both identification and familial testing in the population of south Croatia.
authors.affiliation: Forensic Science Centre "Ivan Vučetić", Zagreb, Croatia.|Forensic Science Office, University of Zagreb, Zagreb, Croatia.
authors.forename: Gordan
authors.initials: G
authors.lastname: Mršić
authors.affiliation: Ruđer Bošković Institute, Zagreb, Croatia.
authors.forename: Petar
authors.initials: P
authors.lastname: Ozretić
authors.affiliation: University Department for Forensic Sciences, University of Split, Split, Croatia.
authors.forename: Josip
authors.initials: J
authors.lastname: Crnjac
authors.affiliation: Forensic Science Centre "Ivan Vučetić", Zagreb, Croatia.|Forensic Science Office, University of Zagreb, Zagreb, Croatia.
authors.forename: Siniša
authors.initials: S
authors.lastname: Merkaš
authors.affiliation: Forensic Science Centre "Ivan Vučetić", Zagreb, Croatia.|Forensic Science Office, University of Zagreb, Zagreb, Croatia.
authors.forename: Ivana
authors.initials: I
authors.lastname: Račić
authors.affiliation: Forensic Science Centre "Ivan Vučetić", Zagreb, Croatia.|Forensic Science Office, University of Zagreb, Zagreb, Croatia.
authors.forename: Sara
authors.initials: S
authors.lastname: Rožić
authors.affiliation: Forensic Science Centre "Ivan Vučetić", Zagreb, Croatia.|Forensic Science Office, University of Zagreb, Zagreb, Croatia.
authors.forename: Viktorija
authors.initials: V
authors.lastname: Sukser
authors.affiliation: Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia.
authors.forename: Maja
authors.initials: M
authors.lastname: Popović
authors.affiliation: Forensic Science Centre "Ivan Vučetić", Zagreb, Croatia. mkorolija@mup.hr.|Forensic Science Office, University of Zagreb, Zagreb, Croatia. mkorolija@mup.hr.
authors.forename: Marina
authors.initials: M
authors.lastname: Korolija
mesh_terms: D041321:Chromosomes, Human, X / Q000235:genetics*; D017523:Croatia; D005260:Female; D005787:Gene Frequency; D005828:Genetics, Population / Q000379:methods*; D006239:Haplotypes; D006801:Humans; D015810:Linkage Disequilibrium; D008297:Male; D018895:Microsatellite Repeats; D044465:White People / Q000235:genetics*
keywords: Forensics; Investigator® Argus X-12; Population study; South Croatia; X-STR