title: Phylogenetic characterization of genes encoding for viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of porcine reproductive & respiratory syndrome virus found in Malaysia in 2013 and 2014.
abstract: BACKGROUND
  Porcine reproductive and respiratory syndrome (PRRS) is one of the most expensive diseases of modern swine production & results in annual economic losses and cost the industry over 600 million USD in U.S. alone and billions of dollars worldwide. Two atypical PRRS cases were observed in 2013 and 2014 characterized by late-term abortion, fever and sudden increase in sow mortality which persisted for a prolonged period of time.
  
  
  METHODS
  Lungs, lymph nodes and other samples were collected for disease investigation. Sequencing of the viral envelope glycoprotein (ORF5) and nucleocapsid protein (ORF7) of PRRSV was done using the BigDye Terminator v3.1 cycle sequencing kit chemistry. The phylogenetic tree was constructed by using the Maximum Likelihood method, generated by Mega 6.06®.
  
  
  RESULTS
  Analysis of the ORF5 and ORF7 showed high degree of sequence homology to PRRSV parent vaccine strain VR-2332, RespPRRSV and other mutant/chimeric virus strains.
  
  
  CONCLUSIONS
  Our study suggests that recombination events between vaccine strains and field isolates may contribute to PRRSV virulence in the field.
authors.affiliation: Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia.|Asia-Pacific Special Nutrients Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
authors.forename: Seetha Jaganathan
authors.initials: SJ
authors.lastname: King
authors.affiliation: Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia. ooi@upm.edu.my.
authors.forename: Peck Toung
authors.initials: PT
authors.lastname: Ooi
authors.affiliation: Department of Biotechnology, Faculty of Biotechnology & Molecular Science, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia.
authors.forename: Lai Yee
authors.initials: LY
authors.lastname: Phang
authors.affiliation: Department of Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia, UPM, Serdang, Selangor, 43400, Malaysia.
authors.forename: Zeenathul Nazariah Binti
authors.initials: ZN
authors.lastname: Allaudin
authors.affiliation: Vet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
authors.forename: Wei Hoong
authors.initials: WH
authors.lastname: Loh
authors.affiliation: Vet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
authors.forename: Chiou Yan
authors.initials: CY
authors.lastname: Tee
authors.affiliation: Vet Food Agro Diagnostic Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
authors.forename: Shiao Pau
authors.initials: SP
authors.lastname: How
authors.affiliation: Vet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
authors.forename: Lai Siong
authors.initials: LS
authors.lastname: Yip
authors.affiliation: Vet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
authors.forename: Pow Yoon
authors.initials: PY
authors.lastname: Choo
authors.affiliation: Vet Food Agro Diagnostic (M) Sdn. Bhd, Lot 18B, Jalan 241, Section 51A, Petaling Jaya, Selangor, 46100, Malaysia.
authors.forename: Ban Keong
authors.initials: BK
authors.lastname: Lim
mesh_terms: D000818:Animals; D015967:Gene Expression Regulation, Viral / Q000502:physiology; D008296:Malaysia / Q000453:epidemiology; D010802:Phylogeny; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology / Q000821:virology*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D013552:Swine; D013997:Time Factors; D014759:Viral Envelope Proteins / Q000235:genetics* / Q000378:metabolism*
chemical_list: D014759:Viral Envelope Proteins; C114789:glycoprotein 5, PRRSV
keywords: Genetic characterization; ORF5 gene; ORF7 gene; PRRSV; Porcine reproductive and respiratory syndrome virus