title: A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering.
abstract: Photo-thermal angular light scattering (PT-AS) is a novel optical method for measuring the hemoglobin concentration ([Hb]) of blood samples. On the basis of the intrinsic photothermal response of hemoglobin molecules, the sensor enables high-sensitivity, chemical-free measurement of [Hb]. [Hb] detection capability with a limit of 0.12 g/dl over the range of 0.35 - 17.9 g/dl has been demonstrated previously. The method can be readily implemented using inexpensive consumer electronic devices such as a laser pointer and a webcam. The use of a micro-capillary tube as a blood container also enables the hemoglobin assay with a nanoliter-scale blood volume and a low operating cost. Here, detailed instructions for the PT-AS optical setup and signal processing procedures are presented. Experimental protocols and representative results for blood samples in anemic conditions ([Hb] = 5.3, 7.5, and 9.9 g/dl) are also provided, and the measurements are compared with those from a hematology analyzer. Its simplicity in implementation and operation should enable its wide adoption in clinical laboratories and resource-limited settings.
authors.affiliation: School of Mechanical Engineering, Yonsei University.
authors.forename: Uihan
authors.initials: U
authors.lastname: Kim
authors.affiliation: Department of Laboratory Medicine, Yonsei University.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Song
authors.affiliation: School of Mechanical Engineering, Yonsei University.
authors.forename: Suho
authors.initials: S
authors.lastname: Ryu
authors.affiliation: School of Mechanical Engineering, Yonsei University.
authors.forename: Soocheol
authors.initials: S
authors.lastname: Kim
authors.affiliation: School of Mechanical Engineering, Yonsei University; cjoo@yonsei.ac.kr.
authors.forename: Chulmin
authors.initials: C
authors.lastname: Joo
mesh_terms: D001681:Biological Assay; D001800:Blood Specimen Collection; D006454:Hemoglobins / Q000032:analysis*; D006801:Humans; D008027:Light
chemical_list: D006454:Hemoglobins