title: Liquid Biopsy in Non-Small Cell Lung Cancer.
abstract: Liquid biopsy analyses are already incorporated in the routine clinical practice in many hospitals and oncology departments worldwide, improving the selection of treatments and monitoring of lung cancer patients. Although they have not yet reached its full potential, liquid biopsy-based tests will soon be as widespread as "standard" biopsies and imaging techniques, offering invaluable diagnostic, prognostic, and predictive information. This review summarizes the techniques available for the isolation and analysis of circulating free DNA and RNA, exosomes, tumor-educated platelets, and circulating tumor cells from the blood of cancer patients, presents the methodological challenges associated with each of these materials, and discusses the clinical applications of liquid biopsy testing in lung cancer.
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Miguel A
authors.initials: MA
authors.lastname: Molina-Vila
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Clara
authors.initials: C
authors.lastname: Mayo-de-Las-Casas
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Giménez-Capitán
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Núria
authors.initials: N
authors.lastname: Jordana-Ariza
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Mónica
authors.initials: M
authors.lastname: Garzón
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Ariadna
authors.initials: A
authors.lastname: Balada
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Sergi
authors.initials: S
authors.lastname: Villatoro
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Teixidó
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Beatriz
authors.initials: B
authors.lastname: García-Peláez
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Cristina
authors.initials: C
authors.lastname: Aguado
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: María José
authors.initials: MJ
authors.lastname: Catalán
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Raquel
authors.initials: R
authors.lastname: Campos
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Ana
authors.initials: A
authors.lastname: Pérez-Rosado
authors.affiliation: Laboratory of Oncology, Pangaea Biotech, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Jordi
authors.initials: J
authors.lastname: Bertran-Alamillo
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Alejandro
authors.initials: A
authors.lastname: Martínez-Bueno
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: María-de-Los-Llanos
authors.initials: MD
authors.lastname: Gil
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: María
authors.initials: M
authors.lastname: González-Cao
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Xavier
authors.initials: X
authors.lastname: González
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Daniela
authors.initials: D
authors.lastname: Morales-Espinosa
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Santiago
authors.initials: S
authors.lastname: Viteri
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital , Barcelona , Spain.
authors.forename: Niki
authors.initials: N
authors.lastname: Karachaliou
authors.affiliation: Instituto Oncológico Dr Rosell, Quirón Dexeus University Hospital, Barcelona, Spain; Cancer Biology and Precision Medicine Program, Catalan Institute of Oncology, Germans Trias i Pujol Health Sciences Institute and Hospital, Badalona, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Rosell
keywords: CTCs; ctDNA; ctRNA; exosomes; gene fusions; lung cancer; mutations; tumor-educated platelets