title: Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007-2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%-99.7% and 80.9%-100%, respectively. Phylogenetic analysis based on theNSP2gene showed that highly pathogenic (HP)-PRRSV was still the dominant virus in southern China from 2013 to 2014. Compared with reference strains CH-1a and VR-2332, the field strain 131101-GD-SHC, which shared high homology with JXA1-P170, had a novel 12 amino acid deletion at position 499-510. Phylogenetic analysis based on theORF5gene showed that HP-PRRSV, VR2332-like strains, and QYYZ-like strains were simultaneously circulating in southern China from 2007 to 2014, suggesting that, in recent years, the type 2 PRRSV was more diverse in southern China. In conclusion, mutations in the decoy epitope and primary neutralizing epitope could be markers of viral evolution and used to study evolutionary relationships among PRRSV strains in China.
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Qiwen
authors.initials: Q
authors.lastname: Wu
authors.affiliation: Henan University of Science and Technology, Luoyang 471000, China.
authors.forename: Zhili
authors.initials: Z
authors.lastname: Li
authors.affiliation: Guangdong Wen's Foodstuff Group Co., Ltd., Yunfu 527400, China.
authors.forename: Guanqun
authors.initials: G
authors.lastname: Zhang
authors.affiliation: Guangdong Wen's Foodstuff Group Co., Ltd., Yunfu 527400, China.
authors.forename: Jianqiang
authors.initials: J
authors.lastname: Niu
authors.affiliation: Guangdong Wen's Foodstuff Group Co., Ltd., Yunfu 527400, China.
authors.forename: Xiduo
authors.initials: X
authors.lastname: Zeng
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Baoli
authors.initials: B
authors.lastname: Sun
authors.affiliation: College of Animal Science, South China Agricultural University, Guangzhou 510642, China.
authors.forename: Jingyun
authors.initials: J
authors.lastname: Ma
mesh_terms: D000818:Animals; D002681:China / Q000453:epidemiology; D014644:Genetic Variation / Q000235:genetics*; D010802:Phylogeny; D016133:Polymerase Chain Reaction / Q000662:veterinary; D019318:Porcine Reproductive and Respiratory Syndrome / Q000453:epidemiology / Q000821:virology; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics*; D016415:Sequence Alignment / Q000662:veterinary; D017422:Sequence Analysis, DNA / Q000662:veterinary; D020539:Sequence Analysis, Protein / Q000662:veterinary; D013552:Swine
keywords: GP5; NSP2; phylogenetic analysis; porcine reproductive and respiratory syndrome virus