title: The effect of capsaicin on expression patterns of CGRP in trigeminal ganglion and trigeminal nucleus caudalis following experimental tooth movement in rats.
abstract: The aim of this study was to explore the effect of capsaicin on expression patterns of calcitonin gene-related peptide (CGRP) in the trigeminal ganglion (TG) and trigeminal subnucleus caudalis (Vc) following experimental tooth movement.
  
  
  
  Male Sprague-Dawley rats were used in this study and divided into small-dose capsaicin+force group, large-dose capsaicin+force group, saline+force group, and no force group. Closed coil springs were used to mimic orthodontic forces in all groups except for the no force group, in which springs were inactivated. Capsaicin and saline were injected into periodontal tissues. Rats were euthanized at 0 h, 12 h, 1 d, 3 d, 5 d, and 7 d following experimental tooth movement. Then, TG and Vc were obtained for immunohistochemical staining and western blotting against CGRP.
  
  
  
  Immunohistochemical results indicated that CGRP positive neurons were located in the TG, and CGRP immunoreactive fibers were distributed in the Vc. Immunohistochemical semiquantitative analysis and western blotting analysis demonstrated that CGRP expression levels both in TG and Vc were elevated at 12 h, 1 d, 3 d, 5 d, and 7 d in the saline + force group. However, both small-dose and large-dose capsaicin could decrease CGRP expression in TG and Vc at 1 d and 3 d following experimental tooth movement, as compared with the saline + force group.
  
  
  
  These results suggest that capsaicin could regulate CGRP expression in TG and Vc following experimental tooth movement in rats.
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Hu
authors.initials: H
authors.lastname: Long
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Niansong
authors.initials: N
authors.lastname: Ye
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Lina
authors.initials: L
authors.lastname: Liao
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Yang
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Fan
authors.initials: F
authors.lastname: Jian
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Sichuan University, West China Hospital of Stomatology, Department of Orthodontics, State Key Laboratory of Oral Diseases, Chengdu, China.
authors.forename: Wenli
authors.initials: W
authors.lastname: Lai
mesh_terms: D000818:Animals; D015153:Blotting, Western; D015740:Calcitonin Gene-Related Peptide / Q000032:analysis / Q000187:drug effects*; D002211:Capsaicin / Q000494:pharmacology*; D005157:Facial Pain; D007150:Immunohistochemistry; D008297:Male; D011897:Random Allocation; D017207:Rats, Sprague-Dawley; D012016:Reference Values; D015203:Reproducibility of Results; D018689:Sensory System Agents / Q000494:pharmacology*; D012965:Sodium Chloride; D013997:Time Factors; D014087:Tooth Movement Techniques / Q000379:methods*; D014275:Trigeminal Caudal Nucleus / Q000737:chemistry / Q000187:drug effects*; D012668:Trigeminal Ganglion / Q000737:chemistry / Q000187:drug effects*
chemical_list: D018689:Sensory System Agents; D012965:Sodium Chloride; D015740:Calcitonin Gene-Related Peptide; D002211:Capsaicin