title: Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division.
abstract: The neuronal apoptosis inhibitory protein (NAIP) is a constituent of the inflammasome and a key component of the innate immune system. Here we use immunofluorescence to position NAIP within the cytokinetic apparatus, contiguous to chromosomal passenger complex (CPC), Centralspindlin, PRC1 and KIF4A. During metaphase, NAIP accumulates in the mitotic spindle poles and is shown in spindle microtubules; in anaphase NAIP is detected in the middle of the central spindle. At the end of cytokinesis, NAIP is localized in the outlying region of the stem body, the center of the intercellular bridge formed between daughter cells prior to cellular abscission. We also describe the sustained presence of NAIP mRNA and protein throughout the cell cycle with a significant increase observed in the G2/M phase. Consistent with a role for NAIP in cytokinesis, NAIP overexpression in HeLa cells promotes the acquisition of a multinuclear phenotype. Conversely, NAIP siRNA gene silencing results in an apoptotic lethal phenotype. Our confocal and super resolution stimulated-emission-depletion (STED) examination of mammalian cell cytokinesis demonstrate a potential new role for NAIP in addition to anti-apoptotic and innate immunology functions.
authors.affiliation: Department of Cell Biology, University of Granada, Granada 18071, Spain.|Biomedical Research Centre, University of Granada, Granada 18100, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Abadía-Molina
authors.affiliation: Department of Cell Biology, University of Granada, Granada 18071, Spain.|Biomedical Research Centre, University of Granada, Granada 18100, Spain.
authors.forename: Virginia
authors.initials: V
authors.lastname: Morón-Calvente
authors.affiliation: Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa ON K1H 8L1, Canada.
authors.forename: Stephen D
authors.initials: SD
authors.lastname: Baird
authors.affiliation: Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa ON K1H 8L1, Canada.
authors.forename: Fahad
authors.initials: F
authors.lastname: Shamim
authors.affiliation: Department of Human DNA Variability, GENYO, Centre for Genomics and Oncological Research, Pfizer, University of Granada, Andalusian Regional Government, Granada, 18016 Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Martín
authors.affiliation: Children's Hospital of Eastern Ontario Research Institute, University of Ottawa, Ottawa ON K1H 8L1, Canada.|Department of Pediatrics, University of Ottawa, Ottawa ON K1H 8L1, Canada.
authors.forename: Alex
authors.initials: A
authors.lastname: MacKenzie
mesh_terms: D018797:Cell Cycle Proteins / Q000378:metabolism; D002470:Cell Survival; D048749:Cytokinesis; D006367:HeLa Cells; D006801:Humans; D016547:Kinesins / Q000378:metabolism; D018613:Microscopy, Confocal; D008938:Mitosis; D051616:Neuronal Apoptosis-Inhibitory Protein / Q000235:genetics* / Q000378:metabolism*; D010641:Phenotype; D008941:Spindle Apparatus / Q000378:metabolism*; D064047:Spindle Poles / Q000378:metabolism; D015854:Up-Regulation
chemical_list: D018797:Cell Cycle Proteins; C496800:NAIP protein, human; D051616:Neuronal Apoptosis-Inhibitory Protein; C116662:PRC1 protein, human; C484228:KIF4A protein, human; D016547:Kinesins