title: Transcription Factors PvERF15 and PvMTF-1 Form a Cadmium Stress Transcriptional Pathway.
abstract: In plants, cadmium (Cd)-responsive transcription factors are key downstream effectors of Cd stress transcriptional pathways, which are capable of converging Cd stress signals through triggering the expression of Cd detoxification genes. However, the upstream transcriptional regulatory pathways that modulate their responses to Cd are less clear. Previously, we identified the bean (Phaseolus vulgaris) METAL RESPONSE ELEMENT-BINDING TRANSCRIPTION FACTOR1 (PvMTF-1) that responds to Cd and confers Cd tolerance in planta. Here, we demonstrate an upstream transcriptional regulation of thePvMTF-1response to Cd Using a yeast one-hybrid system, we cloned the bean ETHYLENE RESPONSE FACTOR15 (PvERF15) that binds to thePvMTF-1promoter.PvERF15was strongly induced by Cd stress, and its overexpression resulted in the up-regulation ofPvMTF-1DNA-protein interaction assays further revealed that PvERF15 binds directly to a 19-bp AC-rich element in thePvMTF-1promoter. The AC-rich element serves as a positive element bound by PvERF15 to activate gene expression. More importantly, knockdown ofPvERF15by RNA interference resulted in reduced Cd-induced expression ofPvMTF-1PvERF15seems to be involved in Cd tolerance, since knockdown ofPvERF15by RNA interference in bean leaf discs decreased Cd tolerance in a transient assay. Since PvERF15 is a component of the Cd stress transcriptional pathway in beans andPvMTF-1is one of its downstream targets, our findings provide a PvERF15/PvMTF-1 transcriptional pathway and thereby contribute to the understanding of Cd stress transcriptional regulatory pathways in plants.
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, China.
authors.forename: Tingting
authors.initials: T
authors.lastname: Lin
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, China.
authors.forename: Wanning
authors.initials: W
authors.lastname: Yang
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Lu
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Science, Capital Normal University, Beijing 100048, China qixiaoting@cnu.edu.cn.
authors.forename: Xiaoting
authors.identifier: 0000-0001-7172-2391
authors.initials: X
authors.lastname: Qi
mesh_terms: D000222:Adaptation, Physiological / Q000235:genetics; D000595:Amino Acid Sequence; D001483:Base Sequence; D015153:Blotting, Western; D002104:Cadmium / Q000378:metabolism / Q000633:toxicity*; D018506:Gene Expression Regulation, Plant / Q000187:drug effects*; D018613:Microscopy, Confocal; D027805:Phaseolus / Q000235:genetics* / Q000378:metabolism; D010940:Plant Proteins / Q000235:genetics* / Q000378:metabolism; D030821:Plants, Genetically Modified; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding; D034622:RNA Interference; D020218:Response Elements / Q000235:genetics; D020133:Reverse Transcriptase Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics; D013312:Stress, Physiological; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism; D020798:Two-Hybrid System Techniques
chemical_list: D010940:Plant Proteins; D014157:Transcription Factors; D002104:Cadmium