title: The effect of sinomenine in diabetic neuropathic pain mediated by the P2X3receptor in dorsal root ganglia.
abstract: Type 2 diabetes mellitus (T2DM) accounts for more than 90% of all cases of diabetes mellitus (DM). Diabetic neuropathic pain (DNP) is a common complication of T2DM. Sinomenine is a natural bioactive component extracted from the Sinomenium acutum and has anti-inflammatory effects. The aim of our study was to investigate the effects of sinomenine on DNP mediated by the P2X3receptor in dorsal root ganglia (DRG). The mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) in T2DM rats were lower than those of control rats. MWT and TWL in T2DM rats treated with sinomenine were higher compared with those in T2DM rats. The expression levels of the P2X3protein and mRNA in T2DM rat DRG were higher compared with those of the control, while those in T2DM rats treated with sinomenine were significantly lower compared with those of the T2DM rats. Sinomenine significantly inhibited P2X3agonist ATP-activated currents in HEK293 cells transfected with the P2X3receptor. Sinomenine decreased the phosphorylation and activation of P38MAPK in T2DM DRG. Therefore, sinomenine treatment may suppress the up-regulated expression and activation of the P2X3receptor and relieve the hyperalgesia potentiated by the activation of P38MAPK in T2DM rats.
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shenqiang
authors.initials: S
authors.lastname: Rao
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shuangmei
authors.initials: S
authors.lastname: Liu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Lifang
authors.initials: L
authors.lastname: Zou
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Tianyu
authors.initials: T
authors.lastname: Jia
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shanhong
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Wu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Zhihua
authors.initials: Z
authors.lastname: Yi
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Shouyu
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xue
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Changshui
authors.initials: C
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Guilin
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China.
authors.forename: Chunping
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Department of Physiology, Medical College of Nanchang University, Nanchang, Jiangxi, 330006, People's Republic of China. liangsd@hotmail.com.
authors.forename: Shangdong
authors.initials: S
authors.lastname: Liang
mesh_terms: D000818:Animals; D003921:Diabetes Mellitus, Experimental; D003924:Diabetes Mellitus, Type 2 / Q000150:complications; D003929:Diabetic Neuropathies; D005727:Ganglia, Spinal / Q000187:drug effects; D057809:HEK293 Cells; D006801:Humans; D006930:Hyperalgesia; D008297:Male; D009019:Morphinans / Q000494:pharmacology*; D017288:Pain Threshold / Q000187:drug effects; D058920:Purinergic P2X Receptor Antagonists / Q000494:pharmacology*; D051381:Rats; D017207:Rats, Sprague-Dawley; D058477:Receptors, Purinergic P2X3 / Q000187:drug effects*
chemical_list: D009019:Morphinans; D058920:Purinergic P2X Receptor Antagonists; D058477:Receptors, Purinergic P2X3; C009271:sinomenine
keywords: Diabetic neuropathic pain; Dorsal root ganglia; P2X3 receptor; Sinomenine