title: Design and control of integrated chromatography column sequences.
abstract: To increase the productivity in biopharmaceutical production, a natural step is to introduce integrated continuous biomanufacturing which leads to fewer buffer and storage tanks, smaller sizes of integrated unit operations, and full automation of the operation. The main contribution of this work is to illustrate a methodology for design and control of a downstream process based on integrated column sequences. For small scale production, for example, pre-clinical studies, integrated column sequences can be implemented on a single chromatography system. This makes for a very efficient drug development platform. The proposed methodology is composed of four steps and is governed by a set of tools, that is presented, that makes the transition from batch separations to a complete integrated separation sequence as easy as possible. This methodology, its associated tools and the physical implementation is presented and illustrated on a case study where the target protein is separated from impurities through an integrated four column sequence. This article shows that the design and control of an integrated column sequence was successfully implemented for a tertiary protein separation problem. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:923-930, 2017.
authors.affiliation: Department of Chemical Engineering, Lund University, P.O. Box 124, SE, 211 00, Lund, Sweden.
authors.forename: Niklas
authors.identifier: http://orcid.org/0000-0002-6032-5494
authors.initials: N
authors.lastname: Andersson
authors.affiliation: Department of Chemical Engineering, Lund University, P.O. Box 124, SE, 211 00, Lund, Sweden.
authors.forename: Anton
authors.initials: A
authors.lastname: Löfgren
authors.affiliation: Department of Chemical Engineering, Lund University, P.O. Box 124, SE, 211 00, Lund, Sweden.
authors.forename: Marianne
authors.initials: M
authors.lastname: Olofsson
authors.affiliation: Department of Chemical Engineering, Lund University, P.O. Box 124, SE, 211 00, Lund, Sweden.
authors.forename: Anton
authors.initials: A
authors.lastname: Sellberg
authors.affiliation: Department of Chemical Engineering, Lund University, P.O. Box 124, SE, 211 00, Lund, Sweden.
authors.forename: Bernt
authors.initials: B
authors.lastname: Nilsson
authors.affiliation: Global Research, Novo Nordisk A/S, Novo Allé, DK, 2880, Bagsvaerd, Denmark.
authors.forename: Peter
authors.initials: P
authors.lastname: Tiainen
mesh_terms: D000595:Amino Acid Sequence; D002623:Chemistry Techniques, Analytical / Q000295:instrumentation*; D002845:Chromatography / Q000295:instrumentation; D004867:Equipment Design; D011506:Proteins / Q000737:chemistry / Q000302:isolation & purification*
chemical_list: D011506:Proteins
keywords: autonomous downstream processing; continuous bioprocessing; integrated column sequences; multiple column control; straight through processing