title: Efficient screening of potential cellulases and hemicellulases produced by Bosea sp. FBZP-16 using the combination of enzyme assays and genome analysis.
abstract: Identification of bacteria that produce carbohydrolytic enzymes is extremely important given the increased demand for these enzymes in many industries. Twenty lignocellulose-degrading bacterial isolates from Algerian compost and different soils were screened for their potential to produce different enzymes involved in biomass deconstruction. Based on 16S rRNA gene sequencing, the isolates belonged to Proteobacteria and Actinobacteria. Differences among species were reflected both as the presence/absence of enzymes or at the level of enzyme activity. Among the most active species, Bosea sp. FBZP-16 demonstrated cellulolytic activity on both amorphous cellulose (CMC) and complex lignocellulose (wheat straw) and was selected for whole-genomic sequencing. The genome sequencing revealed the presence of a complex enzymatic machinery required for organic matter decomposition. Analysis of the enzyme-encoding genes indicated that multiple genes for endoglucanase, xylanase, β-glucosidase and β-mannosidase are present in the genome with enzyme activities displayed by the bacterium, while other enzymes, such as certain cellobiohydrolases, were not detected at the genomic level. This indicates that a combination of functional screening of bacterial cultures with the use of genome-derived information is important for the prediction of potential enzyme production. These results provide insight into their possible exploitation for the production of fuels and chemicals derived from plant biomass.
authors.affiliation: Laboratoire de Microbiologie Appliquée (LMA), Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algeria.|Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences (CAS), Videnska 1083, 14220, Praha 4, Czech Republic.
authors.forename: Aicha Asma
authors.initials: AA
authors.lastname: Houfani
authors.affiliation: Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences (CAS), Videnska 1083, 14220, Praha 4, Czech Republic.
authors.forename: Tomáš
authors.initials: T
authors.lastname: Větrovský
authors.affiliation: Laboratory of Environmental Microbiology, Institute of Microbiology of the Czech Academy of Sciences (CAS), Videnska 1083, 14220, Praha 4, Czech Republic.
authors.forename: Petr
authors.initials: P
authors.lastname: Baldrian
authors.affiliation: Laboratoire de Microbiologie Appliquée (LMA), Département de Microbiologie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algeria. sbenal@hotmail.com.
authors.forename: Said
authors.initials: S
authors.lastname: Benallaoua
mesh_terms: D039903:Actinobacteria / Q000235:genetics / Q000302:isolation & purification; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D002480:Cellulase / Q000235:genetics* / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism; D006026:Glycoside Hydrolases / Q000235:genetics* / Q000378:metabolism*; D008031:Lignin / Q000378:metabolism; D010802:Phylogeny; D020560:Proteobacteria / Q000235:genetics / Q000302:isolation & purification; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012230:Rhizobiaceae / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification*; D017423:Sequence Analysis, RNA / Q000379:methods*; D012987:Soil; D012988:Soil Microbiology
chemical_list: D001426:Bacterial Proteins; D012336:RNA, Ribosomal, 16S; D012987:Soil; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; D006026:Glycoside Hydrolases; C023305:hemicellulase; D002480:Cellulase
keywords: Bosea; Cellulases; Enzyme assays; Genome sequencing; Hemicellulases