title: Transformation and Tumorigenicity Testing of Simian Cell Lines and Evaluation of Poliovirus Replication.
abstract: The key role of cell cultures in different scientific fields is worldwide recognized, both as in vitro research models alternative to laboratory animals and substrates for biological production. However, many safety concerns rise from the use of animal/human cell lines that may be tumorigenic, leading to potential adverse contaminations in cell-derived biologicals. In order to evaluate the suitability of 13 different cell lines for Poliovirus vaccine production, safety and quality, in vitro/in vivo tumorigenicity and Poliovirus propagation properties were evaluated. Our results revealed that non-human primate cell lines CYNOM-K1, FRhK-4, 4MBr-5 and 4647 are free of tumorigenic features and represent highly susceptible substrates for attenuated Sabin Poliovirus strains. In particular, FRhK-4 and 4647 cell lines are characterized by a higher in vitro replication, resulting indicated for the use in large-scale production field.
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Silvia
authors.initials: S
authors.lastname: Dotti
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Tina
authors.initials: T
authors.lastname: Lombardo
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Riccardo
authors.initials: R
authors.lastname: Villa
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Cacciamali
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Cinzia
authors.initials: C
authors.lastname: Zanotti
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Nadia Andrea
authors.initials: NA
authors.lastname: Andreani
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Stefano
authors.initials: S
authors.lastname: Cinotti
authors.affiliation: Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Brescia, Italy.
authors.forename: Maura
authors.initials: M
authors.lastname: Ferrari
mesh_terms: D000818:Animals; D001681:Biological Assay; D063646:Carcinogenesis / Q000473:pathology*; D002460:Cell Line; D002471:Cell Transformation, Neoplastic / Q000473:pathology*; D017955:Poliovirus / Q000502:physiology*; D011323:Primates; D014779:Virus Replication