title: Bio-inspired virus imprinted polymer for prevention of viral infections.
abstract: A novel virus-imprinted polymer for prevention of viral infection was prepared by anchoring molecularly imprinted polymer (MIP) on the surface of poly-dopamine (PDA)-coated silica particles. The imprinting reaction was carried out via self-polymerization of dopamine in the presence of a virus template. Plaque forming assay indicated that the MIP exhibited selective anti-viral infection properties for the template virus in complex media containing different interfering substances, and even other types of viruses. Remarkable dose-dependent and time-dependent inhibition of virus infection was observed due to the MIP's selective binding to the template virus. When the MIP was incubated with the virus and host cells together, rapid and selective adsorption of template viruses by the MIP prevented the viruses to infect the host cells in a period of 12h. The MIP was biocompatible and non-toxic, and had excellent stability and reusability. Furthermore, the MIPs prepared using different viruses as templates showed similar anti-viral infection properties. The MIP synthesized using dopamine as monomer and crude virus as template provided an attractive possibility for clinical applications in the field of antiviral therapy.
  
  
  
  This is the first report to prepare artificial antibody (molecularly imprinted polymer, MIP) that can selectively prevent virus infection using dopamine self-polymerization system. Only MIP anchoring on the surface of poly-dopamine coated silica particles and polymerized using ammonium persulfate as radical initiator showed dose-dependent and time-dependent inhibition of template virus infection in complex media containing interferences and even other viruses. Viruses bond to MIP lost infectious capability. When incubated with virus and host cells, MIP rebond viruses rapidly and selectively to prevent viruses infecting host cells for 12h. The achieved MIPs were biocompatibility, non-toxicity with excellent stability and reusability, and can be used to different viruses. The bio-mimic MIPs provided an attractive prospect for clinical applications in antiviral therapy.
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: lining@hust.edu.cn.
authors.forename: Ning
authors.initials: N
authors.lastname: Li
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: 729789596@qq.com.
authors.forename: Yan-Jie
authors.initials: YJ
authors.lastname: Liu
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: smelly.l@163.com.
authors.forename: Fei
authors.initials: F
authors.lastname: Liu
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: 236694462@qq.com.
authors.forename: Mi-Fang
authors.initials: MF
authors.lastname: Luo
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: 779589528@qq.com.
authors.forename: Ying-Chun
authors.initials: YC
authors.lastname: Wan
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: huangzhg@mails.tjmu.edu.cn.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Huang
authors.affiliation: Jingzhou Center for Disease Control and Prevention, #91 Yuanlin Road, Shashi District, Jingzhou 434000, Hubei, China. Electronic address: 649713739@qq.com.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liao
authors.affiliation: Jingzhou Center for Disease Control and Prevention, #91 Yuanlin Road, Shashi District, Jingzhou 434000, Hubei, China. Electronic address: 1034200085@qq.com.
authors.forename: Fang-Sheng
authors.initials: FS
authors.lastname: Mei
authors.affiliation: Jingzhou Center for Disease Control and Prevention, #91 Yuanlin Road, Shashi District, Jingzhou 434000, Hubei, China. Electronic address: 1300636886@qq.com.
authors.forename: Zhi-Cheng
authors.initials: ZC
authors.lastname: Wang
authors.affiliation: Jingzhou Center for Disease Control and Prevention, #91 Yuanlin Road, Shashi District, Jingzhou 434000, Hubei, China. Electronic address: 645858931@qq.com.
authors.forename: Ai-Yin
authors.initials: AY
authors.lastname: Jin
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: yunshi@hust.edu.cn.
authors.forename: Yun
authors.initials: Y
authors.lastname: Shi
authors.affiliation: Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (Incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, #13 Hangkong Road, Wuhan, Hubei 430030, China. Electronic address: lubin@mails.tjmu.edu.cn.
authors.forename: Bin
authors.initials: B
authors.lastname: Lu
mesh_terms: D040761:Biomimetic Materials / Q000737:chemistry*; D004298:Dopamine / Q000737:chemistry; D006461:Hemolysis; D056945:Hep G2 Cells; D006801:Humans; D054802:Molecular Imprinting / Q000379:methods*; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D012822:Silicon Dioxide / Q000737:chemistry; D017550:Spectroscopy, Fourier Transform Infrared; D014777:Virus Diseases / Q000517:prevention & control*
chemical_list: D011108:Polymers; D012822:Silicon Dioxide; D004298:Dopamine
keywords: Anti-viral infection; Biocompatibility; Dopamine; Toxicity; Virus-imprinted polymer