title: Zinc supplementation of vitrification medium improves in vitro maturation and fertilization of oocytes derived from vitrified-warmed mouse ovaries.
abstract: Oocyte cryopreservation is an approach for fertility preservation for normal women and cancer patients facing chemo and radiotherapy. The present study evaluated the effect of adding zinc chloride to the vitrification medium used for whole mouse ovaries and then assessing the in vitro maturation and fertilization of oocytes when they were subsequently extracted from these vitrified ovarian tissues. Four vitrification solutions with 0, 100,150 and 200 μg/dl zinc (V0, V1, V2 and V3 respectively) were compared. The viability of oocytes isolated from ovaries vitrified-warmed in the highest concentration of zinc (V3) was significantly higher after 24 than in the control V0 group (72.99 vs 85.97). Progression to the MII stage, fertilization and cleavage by 48 h was also higher in the V3 than V0 control group (35.55 vs 44.73), (47.67 vs 63.74), (28.72 vs 43.03) (P < 0.05) respectively. These results indicate that supplementation of vitrification medium for intact ovaries with zinc can improve the oocyte viability and in vitro maturation-fertilization rate.
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Razi University, Taq-e Bostan, Bagh-e Abrisham, 6714967346, Kermanshah, Iran. Electronic address: Geravandi.sh90@yahoo.com.
authors.forename: Shirin
authors.initials: S
authors.lastname: Geravandi
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Razi University, Taq-e Bostan, Bagh-e Abrisham, 6714967346, Kermanshah, Iran. Electronic address: azadbakhtm_tmu@yahoo.com.
authors.forename: Mehri
authors.initials: M
authors.lastname: Azadbakht
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Razi University, Taq-e Bostan, Bagh-e Abrisham, 6714967346, Kermanshah, Iran. Electronic address: Mahsapourmoradi@yahoo.com.
authors.forename: Mahsa
authors.initials: M
authors.lastname: Pourmoradi
authors.affiliation: Department of Biology, Faculty of Basic Sciences, Razi University, Taq-e Bostan, Bagh-e Abrisham, 6714967346, Kermanshah, Iran. Electronic address: f.nowrouzi92@gmail.com.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Nowrouzi
mesh_terms: D000818:Animals; D002454:Cell Differentiation / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D002712:Chlorides / Q000494:pharmacology*; D015925:Cryopreservation / Q000379:methods*; D003451:Cryoprotective Agents / Q000494:pharmacology*; D005260:Female; D059247:Fertility Preservation / Q000379:methods; D005306:Fertilization; D005307:Fertilization in Vitro / Q000379:methods; D051379:Mice; D009865:Oocytes / Q000502:physiology*; D010053:Ovary / Q000187:drug effects; D058989:Vitrification; D017967:Zinc Compounds / Q000494:pharmacology*
chemical_list: D002712:Chlorides; D003451:Cryoprotective Agents; D017967:Zinc Compounds; C016837:zinc chloride
keywords: In vitro fertilization; In vitro maturation; Mouse; Ovary; Vitrification; Zinc