title: Induction of Apoptosis in Normal Human Dermal Fibroblasts Infected with Borrelia burgdorferi Sensu Lato.
abstract: The spirochete Borrelia burgdorferi s.l. can enter into different eukaryotic cells. Intracellular localization of bacteria may cause many changes in different cell pathways like apoptosis-mediated caspase cascade. The present studies focused on gene expression associated with caspase cascade after normal human dermal fibroblasts (NHDF) infection with Borrelia garinii, Borrelia afzelii, and B. burgdorferi s.s. The use of oligonucleotide microarray technique enabled an expression level comparison of genes associated with caspase cascade in NHDF infected with spirochetes. The increased expression of genes associated with caspase cascade was observed in case of CASP5, CASP2, CARD10, CASP10, MALT1, and NLRP1. The decreased expression was observed in case of CASP4, CASP6, and CASP1. The mRNA expression for CASP3 was inhibited in cells infected with three genospecies of Borrelia. However, the intensity of fluorescence was not statistically significant. In addition, cell cultures were fixed and procedure of caspase-3 detection and the TUNEL assay were performed. The in situ caspase-3 detection procedure confirmed the results obtained from microarray analyses. Only several fluorescent signals were observed. Many apoptotic cells were detected in NHDF-infected cultures with all spirochete genospecies found using the TUNEL reaction.
authors.affiliation: 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
authors.forename: Beata
authors.initials: B
authors.lastname: Rozwadowska
authors.affiliation: 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
authors.forename: Marta
authors.initials: M
authors.lastname: Albertyńska
authors.affiliation: 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .|2 Institute of Materials Science, Faculty of Computer Science and Materials Science, University of Silesia in Katowice , Poland .
authors.forename: Hubert
authors.initials: H
authors.lastname: Okła
authors.affiliation: 1 Department of Skin Structural Studies, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
authors.forename: Krzysztof P
authors.initials: KP
authors.lastname: Jasik
authors.affiliation: 2 Institute of Materials Science, Faculty of Computer Science and Materials Science, University of Silesia in Katowice , Poland .
authors.forename: Andrzej S
authors.initials: AS
authors.lastname: Swinarew
authors.affiliation: 3 Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
authors.forename: Urszula
authors.initials: U
authors.lastname: Mazurek
authors.affiliation: 4 Department of Pharmacognosy and Phytochemistry, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia in Katowice , Poland .
authors.forename: Sławomir
authors.initials: S
authors.lastname: Dudek
authors.affiliation: 5 Department of Animal Histology and Embryology, Faculty of Biology and Environmental Protection, University of Silesia in Katowice , Poland .
authors.forename: Danuta
authors.initials: D
authors.lastname: Urbańska-Jasik
authors.affiliation: 5 Department of Animal Histology and Embryology, Faculty of Biology and Environmental Protection, University of Silesia in Katowice , Poland .
authors.forename: Izabela
authors.initials: I
authors.lastname: Poprawa
mesh_terms: D017209:Apoptosis; D015748:Borrelia burgdorferi Group / Q000502:physiology*; D020169:Caspases / Q000235:genetics / Q000378:metabolism; D002478:Cells, Cultured; D005347:Fibroblasts / Q000382:microbiology* / Q000502:physiology*; D005786:Gene Expression Regulation; D006801:Humans; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D012333:RNA, Messenger; D020169:Caspases
keywords: Borrelia spirochetes; TUNEL assay; apoptosis; caspase-3; normal human dermal fibroblasts; oligonucleotide microarray