title: 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts.
abstract: BACKGROUND This study aimed to explore whether 5-aminolaevulinic acid-based photodynamic therapy (ALA-PDT) restrains pathological hyperplasia of fibroblasts from hyperplastic scar tissues, and to investigate the potential mechanism. MATERIAL AND METHODS We used MTT assay, flow cytometry, and terminal-deoxynucleotidyl transferase mediated nick-end labeling (TUNEL) to examine the effects of ALA-PDT on proliferation, cell cycle, and apoptosis of fibroblasts isolated from hyperplastic scar tissues. The growth-promoting effect of fibroblasts on vascular endothelial cells was measured by cell co-culture. Real-time PCR and Western blot analysis were performed to detect the expression levels of transforming growth factor-β1 (TGF-β1), α-smooth muscle actin (a-SMA), Collagen I, Collagen III, vascular endothelial growth factor-A (VEGFA), and basic fibroblast growth factor (bFGF). RESULTS ALA-PDT inhibited proliferation delayed cell cycle progress, promoted apoptosis of fibroblasts, and suppressed its growth-promoting effect on vascular endothelial cells, and decreased expression of TGF-β1, α-SMA, Collagen I, Collagen III, VEGFA, and bFGF. CONCLUSIONS ALA-PDT effectively restrained pathological hyperplasia of fibroblasts from hyperplastic scar tissues, which may provide a research basis for clinical therapy of hyperplastic scars.
authors.affiliation: Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Xiaochuan
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Ping
authors.initials: P
authors.lastname: Cao
authors.affiliation: Department of Hepatobiliary Surgery, The First People's Hospital of Kunming, Kunming, Yunnan, China (mainland).
authors.forename: Jian
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Peng
authors.initials: P
authors.lastname: Du
authors.affiliation: Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Zhiqiong
authors.initials: Z
authors.lastname: Wang
authors.affiliation: Department of Genetics, Kunming University of Science and Technology, Kunming, Yunnan, China (mainland).
authors.forename: Wei
authors.initials: W
authors.lastname: Chen
authors.affiliation: Department of Clinical Medicine, Kunming University of Traditional Chinese Medicine, Kunming, China (mainland).
authors.forename: Chang
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Dermatology, The First People's Hospital of Yunnan Province, Kunming, Yunnan, China (mainland).
authors.forename: Yifei
authors.initials: Y
authors.lastname: Wu
mesh_terms: D000328:Adult; D000622:Aminolevulinic Acid / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002478:Cells, Cultured; D002921:Cicatrix / Q000188:drug therapy* / Q000473:pathology; D003094:Collagen / Q000235:genetics / Q000378:metabolism; D005260:Female; D016222:Fibroblast Growth Factor 2 / Q000378:metabolism; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects* / Q000473:pathology; D006801:Humans; D006965:Hyperplasia / Q000188:drug therapy; D008297:Male; D010778:Photochemotherapy / Q000379:methods*; D055815:Young Adult
chemical_list: D016222:Fibroblast Growth Factor 2; D000622:Aminolevulinic Acid; D003094:Collagen