title: Cu(i) and Ag(i) complex formation with the hydrophilic phosphine 1,3,5-triaza-7-phosphadamantane in different ionic media. How to estimate the effect of a complexing medium.
abstract: The complexes of Cu(i) and Ag(i) with 1,3,5-triaza-7-phosphadamantane (PTA) are currently studied for their potential clinical use as anticancer agents, given the cytotoxicity they exhibited in vitro towards a panel of several human tumor cell lines. These metallodrugs are prepared in the form of [M(PTA)4]+(M = Cu+, Ag+) compounds and dissolved in physiological solution for their administration. However, the nature of the species involved in the cytotoxic activity of the compounds is often unknown. In the present work, the thermodynamics of formation of the complexes of Cu(i) and Ag(i) with PTA in aqueous solution is investigated by means of potentiometric, spectrophotometric and microcalorimetric methods. The results show that both metal(i) ions form up to four successive complexes with PTA. The formation of Ag(i) complexes is studied at 298.15 K in 0.1 M NaNO3whereas the formation of the Cu(i) one is studied in 1 M NaCl, where Cu(i) is stabilized by the formation of three successive chloro-complexes. Therefore, for this latter system, conditional stability constants and thermodynamic data are obtained. To estimate the affinity of Cu(i) for PTA in the absence of chloride, Density Functional Theory (DFT) calculations have been done to obtain the stoichiometry and the relative stability of the possible Cu/PTA/Cl species. Results indicate that one chloride ion is involved in the formation of the first two complexes of Cu(i) ([CuCl(PTA)] and [CuCl(PTA)2]) whereas it is absent in the successive ones ([Cu(PTA)3]+and [Cu(PTA)4]+). The combination of DFT results and thermodynamic experimental data has been used to estimate the stability constants of the four [Cu(PTA)n]+(n = 1-4) complexes in an ideal non-complexing medium. The calculated stability constants are higher than the corresponding conditional values and show that PTA prefers Cu(i) to the Ag(i) ion. The approach used here to estimate the hidden role of chloride on the conditional stability constants of Cu(i) complexes may be applied to any Cu(i)/ligand system, provided that the stoichiometry of the species in NaCl solution is known. The speciation for the two systems shows that the [M(PTA)4]+(M = Cu+, Ag+) complexes present in the metallodrugs are dissociated into lower stoichiometry species when diluted to the micromolar concentration range, typical of the in vitro biological testing. Accordingly, [Cu(PTA)2]+, [Cu(PTA)3]+and [Ag(PTA)2]+are predicted to be the species actually involved in the cytotoxic activity of these compounds.
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Padova, Via Marzolo 1, 35131 Padova, Italy. francesco.endrizzi@kit.edu and Institute for Nuclear Waste Disposal, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
authors.forename: Francesco
authors.identifier: http://orcid.org/0000-0003-0122-6362
authors.initials: F
authors.lastname: Endrizzi
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Padova, Via Marzolo 1, 35131 Padova, Italy. francesco.endrizzi@kit.edu.
authors.forename: Plinio
authors.initials: P
authors.lastname: Di Bernardo
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Padova, Via Marzolo 1, 35131 Padova, Italy. francesco.endrizzi@kit.edu.
authors.forename: Pier Luigi
authors.initials: PL
authors.lastname: Zanonato
authors.affiliation: CNR - ICMATE, Corso Stati Uniti 4, 35127 Padova, Italy.
authors.forename: Francesco
authors.initials: F
authors.lastname: Tisato
authors.affiliation: CNR - ICMATE, Corso Stati Uniti 4, 35127 Padova, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Porchia
authors.affiliation: Dipartimento di Scienze Chimiche, Università di Padova, Via Marzolo 1, 35131 Padova, Italy. francesco.endrizzi@kit.edu.
authors.forename: Abdirisak
authors.identifier: http://orcid.org/0000-0003-0966-1983
authors.initials: A
authors.lastname: Ahmed Isse
authors.affiliation: Dipartimento Politecnico di Ingegneria e Architettura, Università di Udine, Laboratori di Scienze e Tecnologie Chimiche, via Cotonificio 108, 33100 Udine, Italy.
authors.forename: Andrea
authors.initials: A
authors.lastname: Melchior
authors.affiliation: Dipartimento Politecnico di Ingegneria e Architettura, Università di Udine, Laboratori di Scienze e Tecnologie Chimiche, via Cotonificio 108, 33100 Udine, Italy.
authors.forename: Marilena
authors.initials: M
authors.lastname: Tolazzi