title: Better Management of Alcohol Liver Disease Using a 'Microstructured Synbox' System Comprising L. plantarum and EGCG.
abstract: Synergistic combination of probiotics with carbohydrate based prebiotics is widely employed for the treatment of various gut related disorders. However, such carbohydrate based prebiotics encourage the growth of pathogens and probiotics, equally. Aim of the study was (i) to explore the possibility of using epigallocatechin gallate (EGCG) a phenolic compound, as a prebiotic for L.plantarum; (ii) to develop and evaluate a microstructured synbox (microencapsulating both probiotic and EGCG together) in rat model of alcohol liver disease (ALD); and, (iii) to confirm whether the combination can address issues of EGCG bioavailability and probiotic survivability in adverse gut conditions. Growth enhancing effect of EGCG on L. plantarum (12.8±0.5 log 10 units) was significantly (p≤0.05) better than inulin (11.4±0.38 log 10 units), a natural storage carbohydrate. The formulated synbox significantly modulated the levels of alcohol, endotoxin, hepatic enzymes and restored the hepatoarchitecture in comparison to simultaneous administration of free agents. Additionally, using a battery of techniques, levels of various cellular and molecular markers viz. NF-kB/p50, TNF-α, IL12/p40, and signalling molecules TLR4, CD14, MD2, MyD88 and COX-2 were observed to be suppressed. Developed microbead synbox, as a single delivery system for both the agents showed synergism and hence, holds promise as a therapeutic option for ALD management.
authors.affiliation: Department of Microbiology, Basic Medical Sciences Block, South Campus, Panjab University, Chandigarh, India.
authors.forename: Praveen
authors.initials: P
authors.lastname: Rishi
authors.affiliation: Department of Microbiology, Basic Medical Sciences Block, South Campus, Panjab University, Chandigarh, India.
authors.forename: Sumeha
authors.initials: S
authors.lastname: Arora
authors.affiliation: Department of Microbiology, Basic Medical Sciences Block, South Campus, Panjab University, Chandigarh, India.
authors.forename: Ujjwal Jit
authors.initials: UJ
authors.lastname: Kaur
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
authors.forename: Kanwaljit
authors.initials: K
authors.lastname: Chopra
authors.affiliation: University Institute of Pharmaceutical Sciences, Panjab University, Chandigarh, India.
authors.forename: Indu Pal
authors.initials: IP
authors.lastname: Kaur
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000008:administration & dosage / Q000627:therapeutic use*; D002392:Catechin / Q000008:administration & dosage / Q000031:analogs & derivatives* / Q000627:therapeutic use; D004338:Drug Combinations; D004339:Drug Compounding; D004357:Drug Synergism; D000431:Ethanol / Q000008:administration & dosage / Q000097:blood; D005260:Female; D048191:Lactiplantibacillus plantarum / Q000187:drug effects / Q000254:growth & development; D008108:Liver Diseases, Alcoholic / Q000188:drug therapy*; D010539:Permeability; D056692:Prebiotics / Q000008:administration & dosage*; D019936:Probiotics / Q000008:administration & dosage* / Q000627:therapeutic use; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D004338:Drug Combinations; D056692:Prebiotics; D000431:Ethanol; D002392:Catechin; C045651:epigallocatechin gallate