title: Analysis of cytokinesis by electron microscopy.
abstract: Following up on a chapter on the Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis (MCB 79, 101-119), we present an adaptation of our established protocol for the ultrastructural analysis of either permeabilized or injected embryonic systems. We prepared both drug-treated early C. elegans embryos and fluorescently labeled sea urchin embryos of Lytechinus pictus for ultrastructural studies on animal cytokinesis. Here we focus on the initial preparation steps of postmitotic embryos for high-pressure freezing and subsequent electron microscopy with an emphasis on electron tomography. The advantages and limitations of our extended protocol will be discussed.
authors.affiliation: Technische Universität Dresden, Dresden, Germany.
authors.forename: J
authors.initials: J
authors.lastname: König
authors.affiliation: European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Borrego-Pinto
authors.affiliation: Technische Universität Dresden, Dresden, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Streichert
authors.affiliation: Technische Universität Dresden, Dresden, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Munzig
authors.affiliation: European Molecular Biology Laboratory (EMBL), Heidelberg, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Lenart
authors.affiliation: Technische Universität Dresden, Dresden, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Müller-Reichert
mesh_terms: D000818:Animals; D017173:Caenorhabditis elegans / Q000648:ultrastructure; D048749:Cytokinesis / Q000235:genetics*; D055032:Electron Microscope Tomography / Q000379:methods*; D004625:Embryo, Nonmammalian / Q000648:ultrastructure*; D047108:Embryonic Development / Q000235:genetics; D008854:Microscopy, Electron / Q000379:methods*; D008938:Mitosis / Q000235:genetics; D012617:Sea Urchins / Q000235:genetics / Q000254:growth & development / Q000648:ultrastructure
keywords: Abscission; Correlative light electron microscopy; Cytokinesis; Electron microscopy; Electron tomography; High-pressure freezing