title: Geroprotective and Radioprotective Activity of Quercetin, (-)-Epicatechin, and Ibuprofen inDrosophila melanogaster.
abstract: The modulation of longevity genes and aging-associated signaling pathways using pharmacological agents is one of the potential ways to prolong the lifespan and increase the vitality of an organism. Phytochemicals flavonoids and non-steroidal anti-inflammatory drugs have a large potential as geroprotectors. The goal of the present study was to investigate the effects of long-term and short-term consumption of quercetin, (-)-epicatechin, and ibuprofen on the lifespan, resistance to stress factors (paraquat, hyperthermia, γ-radiation, and starvation), as well as age-dependent physiological parameters (locomotor activity and fecundity) ofDrosophila melanogaster. The long-term treatment with quercetin and (-)-epicatechin didn't change or decreased the lifespan of males and females. In contrast, the short-term treatment with flavonoids had a beneficial effect and stimulated the resistance to paraquat and acute γ-irradiation. The short-term ibuprofen consumption had a positive effect on the lifespan of females when it was carried out at the middle age (30-40 days), and to the survival of flies under conditions of oxidative and genotoxic stresses. However, it didn't change the lifespan of males and females after the treatment during first 10 days of an imago life. Additionally, quercetin, (-)-epicatechin, and ibuprofen decreased the spontaneous locomotor activity of males, but had no effect of stimulated the physical activity and fecundity of females. Revealed quercetin, (-)-epicatechin, and ibuprofen activity can be associated with the stimulation of stress response mechanisms through the activation of pro-longevity pathways, or the induction of hormesis.
authors.affiliation: Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences (RAS) Syktyvkar, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Proshkina
authors.affiliation: Laboratory of Genetics of Aging and Longevity, Moscow Institute of Physics and Technology (MIPT) Dolgoprudny, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Lashmanova
authors.affiliation: Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences (RAS) Syktyvkar, Russia.
authors.forename: Eugenia
authors.initials: E
authors.lastname: Dobrovolskaya
authors.affiliation: Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences (RAS)Syktyvkar, Russia; Department of Ecology, Institute of Natural Sciences, Syktyvkar State UniversitySyktyvkar, Russia.
authors.forename: Nadezhda
authors.initials: N
authors.lastname: Zemskaya
authors.affiliation: Engelhardt Institute of Molecular Biology (EIMB), Russian Academy of Sciences (RAS) Moscow, Russia.
authors.forename: Anna
authors.initials: A
authors.lastname: Kudryavtseva
authors.affiliation: Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences (RAS)Syktyvkar, Russia; Department of Ecology, Institute of Natural Sciences, Syktyvkar State UniversitySyktyvkar, Russia.
authors.forename: Mikhail
authors.initials: M
authors.lastname: Shaposhnikov
authors.affiliation: Institute of Biology, Komi Science Center, Ural Branch, Russian Academy of Sciences (RAS)Syktyvkar, Russia; Laboratory of Genetics of Aging and Longevity, Moscow Institute of Physics and Technology (MIPT)Dolgoprudny, Russia; Department of Ecology, Institute of Natural Sciences, Syktyvkar State UniversitySyktyvkar, Russia; Engelhardt Institute of Molecular Biology (EIMB), Russian Academy of Sciences (RAS)Moscow, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Moskalev
keywords: (-)-epicatechin; Caenorhabditis elegans; Drosophila melanogaster; ibuprofen; lifespan; quercetin; stress resistance