title: Daily Intake of High-Fat Diet with Lysophosphatidic Acid-Rich Soybean Phospholipids Augments Colon Tumorigenesis in Kyoto Apc Delta Rats.
abstract: Oral administration of lysophosphatidic acid (LPA) was shown to attenuate gastric ulceration in rats and mice but aggravate intestinal tumorigenesis in mice.
  
  
  
  The present study examined whether dietary LPA induces or prevents development of colorectal tumor in rats.
  
  
  
  Kyoto Apc Delta rats fed high-fat diet with or without an LPA-rich soybean phospholipid mixture (LSP, 0.1 or 1%) were treated with azoxymethane and dextran sodium sulfate to induce colorectal tumorigenesis. Rats were killed 15 weeks after azoxymethane treatment, and size, total number, location, and severity of colorectal tumors were assessed. Expression of mRNA of LPA receptors in rat colon tissue was assayed.
  
  
  
  Rats fed the diet supplemented with 1% LSP had a higher number of tumors 2-4 mm long compared than those with or without 0.1% LSP. The mean distance of tumors >4 mm long from the anus was significantly higher than those of tumors <2 and 2-4 mm long in rats fed 1% LSP-supplemented diet. Supplementation of the diet with 0.1% LSP decreased mRNA expression of LPA5 in colon tumors of rats.
  
  
  
  Dietary supplementation of LPA-rich phospholipids dose-dependently augmented colorectal tumorigenesis. Decreased expression of LPA5 in colon tumors may be relevant to augmented tumorigenesis.
authors.affiliation: Department of Pharmaceutical Sciences, Kyushu University of Health and Welfare, 1714-1 Yoshinomachi, Nobeoka, Japan. t-tsutsumi@phoenix.ac.jp.
authors.forename: Toshihiko
authors.initials: T
authors.lastname: Tsutsumi
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, Tokushima, Japan.
authors.forename: Manami
authors.initials: M
authors.lastname: Inoue
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, Tokushima, Japan.
authors.forename: Yoko
authors.initials: Y
authors.lastname: Okamoto
authors.affiliation: Department of Anatomic Pathology, Prefectural Nobeoka Hospital, 2-1-10 Shinkoji, Nobeoka, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Ishihara
authors.affiliation: Department of Pharmaceutical Health Chemistry, Institute of Health Biosciences, University of Tokushima Graduate School, 1-78-1 Shomachi, Tokushima, Japan.|Department of Life Sciences, Faculty of Pharmacy, Yasuda Women's University, 6-13-1 Yasuhigashi Asaminami-ku, Hiroshima, Japan.
authors.forename: Akira
authors.initials: A
authors.lastname: Tokumura
mesh_terms: D000818:Animals; D001397:Azoxymethane / Q000494:pharmacology*; D063646:Carcinogenesis / Q000378:metabolism*; D002273:Carcinogens / Q000494:pharmacology; D002471:Cell Transformation, Neoplastic / Q000378:metabolism; D003106:Colon / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D003110:Colonic Neoplasms / Q000378:metabolism / Q000473:pathology; D045424:Complex Mixtures; D016264:Dextran Sulfate / Q000494:pharmacology*; D059305:Diet, High-Fat / Q000009:adverse effects / Q000379:methods; D004195:Disease Models, Animal; D004305:Dose-Response Relationship, Drug; D018565:Food-Drug Interactions; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008246:Lysophospholipids / Q000494:pharmacology*; D051381:Rats; D013025:Glycine max; D013223:Statistics as Topic
chemical_list: D002273:Carcinogens; D045424:Complex Mixtures; D008246:Lysophospholipids; D016264:Dextran Sulfate; D001397:Azoxymethane; C032881:lysophosphatidic acid
keywords: Azoxymethane/dextran sodium sulfate; Colorectal cancer; Dietary administration; Lysophosphatidic acid; Tumor location