title: CD95 Stimulation with CD95L and DISC Analysis.
abstract: CD95 and its ligand CD95L play a major role in immune surveillance and homeostasis. CD95L is expressed by activated T lymphocytes and NK cells to induce apoptosis in cancer and virus-infected cells. The goal of this chapter is to describe a method used to immunoprecipitate CD95 and analyze its associated protein complex in cells stimulated with a cytotoxic CD95L (i.e., Ig-CD95L).
authors.affiliation: Centre Eugène Marquis, rue Bataille Flandres Dunkerque, 35042, Rennes, France.|INSERM ERL440-OSS, Equipe Labellisée Ligue Contre Le Cancer, Rennes, France.|Université de Rennes-1, 2 av. du Prof. Léon Bernard, 35043, Rennes, France.
authors.forename: Matthieu
authors.initials: M
authors.lastname: Le Gallo
authors.affiliation: Centre Eugène Marquis, rue Bataille Flandres Dunkerque, 35042, Rennes, France. patrick.legembre@inserm.fr.|INSERM ERL440-OSS, Equipe Labellisée Ligue Contre Le Cancer, Rennes, France. patrick.legembre@inserm.fr.|Université de Rennes-1, 2 av. du Prof. Léon Bernard, 35043, Rennes, France. patrick.legembre@inserm.fr.
authors.forename: Patrick
authors.initials: P
authors.lastname: Legembre
mesh_terms: D017209:Apoptosis; D015153:Blotting, Western; D053418:Death Domain Receptor Signaling Adaptor Proteins / Q000378:metabolism*; D053222:Fas Ligand Protein / Q000378:metabolism*; D047468:Immunoprecipitation; D046912:Multiprotein Complexes / Q000378:metabolism; D011485:Protein Binding; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D053418:Death Domain Receptor Signaling Adaptor Proteins; D053222:Fas Ligand Protein; D046912:Multiprotein Complexes; D019014:fas Receptor
keywords: Apoptosis; Death receptors; Fas; FasL; Immunoprecipitation