title: Antioxidant Capacity Determination in Plants and Plant-Derived Products: A Review.
abstract: The present paper aims at reviewing and commenting on the analytical methods applied to antioxidant and antioxidant capacity assessment in plant-derived products. Aspects related to oxidative stress, reactive oxidative species' influence on key biomolecules, and antioxidant benefits and modalities of action are discussed. Also, the oxidant-antioxidant balance is critically discussed. The conventional and nonconventional extraction procedures applied prior to analysis are also presented, as the extraction step is of pivotal importance for isolation and concentration of the compound(s) of interest before analysis. Then, the chromatographic, spectrometric, and electrochemical methods for antioxidant and antioxidant capacity determination in plant-derived products are detailed with respect to their principles, characteristics, and specific applications. Peculiarities related to the matrix characteristics and other factors influencing the method's performances are discussed. Health benefits of plants and derived products are described, as indicated in the original source. Finally, critical and conclusive aspects are given when it comes to the choice of a particular extraction procedure and detection method, which should consider the nature of the sample, prevalent antioxidant/antioxidant class, and the mechanism underlying each technique. Advantages and disadvantages are discussed for each method.
authors.affiliation: Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Splaiul Independentei, Sector 5, 050097 Bucharest, Romania.
authors.forename: Aurelia Magdalena
authors.identifier: 0000-0002-9697-4429
authors.initials: AM
authors.lastname: Pisoschi
authors.affiliation: Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Splaiul Independentei, Sector 5, 050097 Bucharest, Romania.
authors.forename: Aneta
authors.initials: A
authors.lastname: Pop
authors.affiliation: Faculty of Land Reclamation and Environmental Engineering, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 59 Marasti Blvd, Sector 1, 011464 Bucharest, Romania.
authors.forename: Carmen
authors.initials: C
authors.lastname: Cimpeanu
authors.affiliation: Faculty of Veterinary Medicine, University of Agronomic Sciences and Veterinary Medicine of Bucharest, 105 Splaiul Independentei, Sector 5, 050097 Bucharest, Romania.
authors.forename: Gabriel
authors.initials: G
authors.lastname: Predoi
mesh_terms: D053000:Analytic Sample Preparation Methods; D000975:Antioxidants / Q000032:analysis* / Q000302:isolation & purification; D064209:Phytochemicals / Q000032:analysis*; D010936:Plant Extracts / Q000737:chemistry; D010944:Plants / Q000737:chemistry*
chemical_list: D000975:Antioxidants; D064209:Phytochemicals; D010936:Plant Extracts