title: [The effect and mechanism investigation of intermedin on the apoptosis of bone marrow derived mesenchymal stem cells induced by hydrogen peroxide].
abstract: Objective:To investigate the effect and mechanism of intermedin (IMD)on the apoptosis of BMSCs induced by H2O2and its mechanism.Methods:BMSCs being cultured in vitro. After 30 min pretreatment with 10-6mol/L IMD, cells were cultured in presence of 100 μmol/L H2O2for 2 hour, cell apoptosis was determined by flow cytometry; Apoptosis related protein expression was determined by Western blotting and immunofluorescence; Protein and phosphorylation of ERK1/2 was detected by Western blotting and observe the influence of IMD after pretreatment with inhibitor of ERK1/2.Results:IMD could increase phosphorylation of ERK1/2 pathway of BMSCs (P<0.01). Pretreatment of IMD could decrease BMSCs apoptosis induced by H2O2, and up regulation expression of Caspase-3 and Bax, down regulation expression of Bcl-2 (P<0.01), and the effect was abrogated by U0126.Conclusion:IMD can activate ERK1/2 signaling pathway of BMSCs, and can regulate the expression of apoptosis related proteins. Furthermore, it can protect BMSCs from apoptosis induced by H2O2. Activation of ERK1/2 pathway was involved in the effect of IMD on apoptosis.
authors.affiliation: Department of Cardiology, Affiliated Hospital of Zunyi Medical University, Guizhou Zunyi 563001, China.
authors.forename: D M
authors.initials: DM
authors.lastname: Wang
authors.forename: J
authors.initials: J
authors.lastname: Li
authors.forename: W M
authors.initials: WM
authors.lastname: Chen
authors.forename: X P
authors.initials: XP
authors.lastname: Long
authors.forename: B
authors.initials: B
authors.lastname: Shi
authors.forename: R Z
authors.initials: RZ
authors.lastname: Zhao
mesh_terms: D017209:Apoptosis; D001853:Bone Marrow; D001854:Bone Marrow Cells; D053148:Caspase 3 / Q000378:metabolism; D006801:Humans; D006861:Hydrogen Peroxide / Q000494:pharmacology*; D059630:Mesenchymal Stem Cells; D048052:Mitogen-Activated Protein Kinase 3 / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D010766:Phosphorylation; D015398:Signal Transduction
chemical_list: D006861:Hydrogen Peroxide; D048052:Mitogen-Activated Protein Kinase 3; D020928:Mitogen-Activated Protein Kinases; C505407:CASP3 protein, human; D053148:Caspase 3