title: In vitro antiprotozoal activity of (S)-cis-Verbenol against Leishmania spp. and Trypanosoma cruzi.
abstract: (S)-cis-Verbenol, a monoterpene frequently found as a component of essential oils, was assayed against Leishmania amazonensis, Leishmania infantum, Leishmania brasiliensis and against two strains of Trypanosoma cruzi. The cytotoxicity of the compound was also assayed against human fibroblast cells using a colorimetric method. Benznidazole was used as reference drug against T. cruzi and amphotericin B was used against Leishmania spp. The compound showed good activity against the trypanosomes, being more active against the CL Brenner strain, with an IC50value of 8.3μg/mL. Against Leishmania, the IC50values were between 2.1 and 3.8μg/mL. The compound showed no cytotoxicity against human fibroblasts at the concentrations assayed and was 100-500 times more toxic for the parasites than for the human cells, as indicated by the selectivity indexes. The results open interesting perspectives about the potential of (S)-cis-Verbenol and other individual components of essential oils for the treatment of these diseases.
authors.affiliation: Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asunción, Dr. Cecilio Báez c/ Dr. Gaspar Villamayor, Campus Universitario de San Lorenzo Km 9.5, San Lorenzo, Paraguay.
authors.forename: Gloria
authors.initials: G
authors.lastname: Yaluff
authors.affiliation: Centro para el Desarrollo de la Investigación Científica, Manduvirá 635 e/ 15 de Agosto y O' Leary, Asuncion, Paraguay.
authors.forename: Celeste
authors.initials: C
authors.lastname: Vega
authors.affiliation: Departamento de Fitoquímica, Facultad de Ciencias Químicas, Universidad Nacional de Asunción, Dr. José Decoud c/ Escuela Agrícola Mcal. López, Campus Universitario de San Lorenzo Km 9.5, San Lorenzo, Paraguay. Electronic address: nelson@qui.una.py.
authors.forename: Nelson
authors.initials: N
authors.lastname: Alvarenga
mesh_terms: D000818:Animals; D000981:Antiprotozoal Agents / Q000494:pharmacology*; D000081008:Bicyclic Monoterpenes; D014355:Chagas Disease / Q000188:drug therapy / Q000469:parasitology; D005347:Fibroblasts / Q000187:drug effects / Q000469:parasitology; D006801:Humans; D020128:Inhibitory Concentration 50; D007891:Leishmania / Q000187:drug effects*; D007896:Leishmaniasis / Q000188:drug therapy / Q000469:parasitology; D039821:Monoterpenes / Q000737:chemistry* / Q000494:pharmacology*; D009822:Oils, Volatile / Q000494:pharmacology*; D010239:Paraguay; D010936:Plant Extracts / Q000494:pharmacology*; D014349:Trypanosoma cruzi / Q000187:drug effects*
chemical_list: D000981:Antiprotozoal Agents; D000081008:Bicyclic Monoterpenes; D039821:Monoterpenes; D009822:Oils, Volatile; D010936:Plant Extracts; C009951:verbenol
keywords: Antiprotozoal; IC(50); Leishmania; Trypanosoma cruzi; cis-Verbenol