title: Identification and Characterization of Glycopeptides from Egg Protein Ovomucin with Anti-Agglutinating Activity against Porcine K88 Enterotoxigenic Escherichia coli Strains.
abstract: Ovomucin is a glycoprotein from egg white with potential to act as an anti-adhesive agent against infectious diseases. This study aimed to determine whether ovomucin or ovomucin hydrolysates could prevent adhesion of two porcine K88 enterotoxigenic Escherichia coli (ETEC) strains. Adhesion was assessed in vitro using a hemagglutination assay. Ovomucin hydrolysates, but not intact ovomucin, prevented adhesion of ETEC to porcine erythrocytes. The ovomucin hydrolysate prepared by acid protease II exhibited the best anti-agglutinating activity against both strains; this hydrolysate was fractionated by cation exchange chromatography and reverse-phase high-performance liquid chromatography (HPLC). The most active fractions, F3(9) and F7(1), with minimal inhibitory concentrations of 0.03 and 0.25 g/L against strains ECL13795 and ECL13998, respectively, were subjected to structural characterization. Six identified glycopeptides were all derived from α-ovomucin, composed of a pentasaccharide core of two N-acetylglucosamine and three mannose residues (GlcNAc2Man3) and a bisecting N-acetylglucosamine (GlcNAc). The terminal β-linked galactose from these glycopeptides could be one of the binding sites for K88acfimbriae.
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta , 4-10 Ag/For Building, Edmonton, Alberta, Canada T6G 2P5.
authors.forename: Xiaohong
authors.identifier: http://orcid.org/0000-0002-2346-0347
authors.initials: X
authors.lastname: Sun
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta , 4-10 Ag/For Building, Edmonton, Alberta, Canada T6G 2P5.
authors.forename: Michael
authors.identifier: http://orcid.org/0000-0003-0972-928X
authors.initials: M
authors.lastname: Gänzle
authors.affiliation: Department of Agricultural, Food and Nutritional Science, University of Alberta , 4-10 Ag/For Building, Edmonton, Alberta, Canada T6G 2P5.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wu
mesh_terms: D018830:Adhesins, Escherichia coli / Q000235:genetics / Q000378:metabolism; D000371:Agglutination / Q000187:drug effects; D000818:Animals; D001422:Bacterial Adhesion / Q000187:drug effects*; D002645:Chickens; D054307:Enterotoxigenic Escherichia coli / Q000187:drug effects* / Q000235:genetics / Q000502:physiology; D004927:Escherichia coli Infections / Q000188:drug therapy* / Q000382:microbiology; D029968:Escherichia coli Proteins; D006020:Glycopeptides / Q000008:administration & dosage* / Q000737:chemistry / Q000302:isolation & purification; D007413:Intestinal Mucosa / Q000382:microbiology; D010059:Ovomucin / Q000008:administration & dosage / Q000737:chemistry*; D013552:Swine; D013553:Swine Diseases / Q000188:drug therapy* / Q000382:microbiology
chemical_list: D018830:Adhesins, Escherichia coli; D029968:Escherichia coli Proteins; D006020:Glycopeptides; D010059:Ovomucin
keywords: anti-adhesive agent; anti-agglutinating activity; glycopeptides; ovomucin; β-linked galactose