title: Physico-chemical characterization and pharmacological activities of sulfated polysaccharide from sea urchin, Paracentrotus lividus.
abstract: Sulfated polysaccharide (SP) from the eggs of sea urchin Paracentrotus lividus, extracted by papain digestion, was characterized by size exclusion chromatography coupling on-line with light scattering and viscosity detectors (SEC/MALS/VD/DRI), gas chromatography coupled to mass spectrometer (GC-MS), and Fourier transform infrared spectroscopy (FTIR) analysis. The native molecular mass of the extracted polysaccharide is high (≥22 000 KDa) and it is composed mainly of arabinose, accompanied by other monosaccharides (mostly galactose, glucose and fucose), significant amounts of uronic acids (18.4%) and relatively high proportions of sulfate (22.4%). The pharmacological evaluation of SP showed a significant in vivo anti-inflammatory activity (p<0.001), 3h after injection, the edema inhibition was 75.8% at the dose of 100mg/Kg; a significant peripheral analgesic activity (p<0.001), with 64.9% of writhing inhibition, and a significant increase in the hot plate reaction time in mice indicating central analgesic activity. In addition, an interesting gastroprotective effect was observed with this polysaccharide; the gastric ulcer inhibition was 69.7%, at the dose of 100mg/Kg.
authors.affiliation: Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisie.
authors.forename: Yosra Ben
authors.initials: YB
authors.lastname: Salem
authors.affiliation: Laboratoire de Développement Chimique, Galénique et Pharmacologique des Médicaments, Faculté de Pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisie.
authors.forename: Safa
authors.initials: S
authors.lastname: Amri
authors.affiliation: Laboratoire des Plantes Aromatiques et Médicinales (LPAM), Centre de Biotechnologie de Borj- Cédria, BP 901, 2050 Hammam-lif, Tunisie.
authors.forename: Khaoula Mkadmini
authors.initials: KM
authors.lastname: Hammi
authors.affiliation: Laboratoire de Développement Chimique, Galénique et Pharmacologique des Médicaments, Faculté de Pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisie.
authors.forename: Amal
authors.initials: A
authors.lastname: Abdelhamid
authors.affiliation: Normandie Univ, UNIROUEN, INSA Rouen, CNRS, PBS, 76000 Rouen, France.
authors.forename: Didier Le
authors.initials: DL
authors.lastname: Cerf
authors.affiliation: Laboratoire de Développement Chimique, Galénique et Pharmacologique des Médicaments, Faculté de Pharmacie de Monastir, Université de Monastir, 5000 Monastir, Tunisie.
authors.forename: Abderrahman
authors.initials: A
authors.lastname: Bouraoui
authors.affiliation: Laboratoire des Interfaces et des Matériaux Avancés (LIMA), Faculté des Sciences de Monastir, Université de Monastir, Bd. de l'environnement, 5019 Monastir, Tunisie. Electronic address: hatemmajdoub.fsm@gmail.com.
authors.forename: Hatem
authors.initials: H
authors.lastname: Majdoub
mesh_terms: D000700:Analgesics / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000737:chemistry / Q000494:pharmacology / Q000627:therapeutic use; D055598:Chemical Phenomena; D004487:Edema / Q000188:drug therapy; D051379:Mice; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis; D047328:Paracentrotus / Q000737:chemistry*; D011134:Polysaccharides / Q000737:chemistry* / Q000494:pharmacology* / Q000627:therapeutic use; D051381:Rats; D013431:Sulfates / Q000737:chemistry*
chemical_list: D000700:Analgesics; D000894:Anti-Inflammatory Agents, Non-Steroidal; D009005:Monosaccharides; D011134:Polysaccharides; D013431:Sulfates
keywords: Paracentrotus lividus; Pharmacological activities; Physico-chemical characterization; Sulfated polysaccharide