title: Interactome of E. piscicida and grouper liver proteins reveals strategies of bacterial infection and host immune response.
abstract: The occurrence of infectious diseases is related to heterogeneous protein interactions between a host and a microbe. Therefore, elucidating the host-pathogen interplay is essential. We previously revealed the protein interactome between Edwardsiella piscicida and fish gill cells, and the present study identified the protein interactome between E. piscicida and E. drummondhayi liver cells. E. drummondhayi liver cells and bacterial pull-down approaches were used to identify E. piscicida outer membrane proteins that bind to liver cells and fish liver cell proteins that interact with bacterial cells, respectively. Eight bacterial proteins and 11 fish proteins were characterized. Heterogeneous protein-protein interactions between these bacterial cells and fish liver cells were investigated through far-Western blotting and co-immunoprecipitation. A network was constructed based on 42 heterogeneous protein-protein interactions between seven bacterial proteins and 10 fish proteins. A comparison of the new interactome with the previously reported interactome showed that four bacterial proteins overlapped, whereas all of the identified fish proteins were new, suggesting a difference between bacterial tricks for evading host immunity and the host strategy for combating bacterial infection. Furthermore, these bacterial proteins were found to regulate the expression of host innate immune-related proteins. These findings indicate that the interactome contributes to bacterial infection and host immunity.
authors.affiliation: Center for Proteomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquat Food Safety, School of Life Sciences, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
authors.forename: Hui
authors.initials: H
authors.lastname: Li
authors.affiliation: Center for Proteomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquat Food Safety, School of Life Sciences, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
authors.forename: Qing-Feng
authors.initials: QF
authors.lastname: Zhu
authors.affiliation: Center for Proteomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquat Food Safety, School of Life Sciences, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
authors.forename: Xuan-Xian
authors.initials: XX
authors.lastname: Peng
authors.affiliation: Center for Proteomics, State Key Laboratory of Bio-Control, MOE Key Lab Aquat Food Safety, School of Life Sciences, Guangdong Province Key Laboratory for Pharmaceutical Functional Genes, Sun Yat-sen University, University City, Guangzhou 510006, People's Republic of China.
authors.forename: Bo
authors.initials: B
authors.lastname: Peng
mesh_terms: D000818:Animals; D001425:Bacterial Outer Membrane Proteins / Q000378:metabolism*; D020594:Edwardsiella / Q000378:metabolism / Q000472:pathogenicity*; D004756:Enterobacteriaceae Infections / Q000378:metabolism; D005393:Fish Diseases / Q000378:metabolism; D029941:Fish Proteins / Q000378:metabolism*; D054884:Host-Pathogen Interactions; D008099:Liver / Q000378:metabolism* / Q000382:microbiology; D010473:Perciformes / Q000276:immunology / Q000378:metabolism / Q000382:microbiology*; D011485:Protein Binding
chemical_list: D001425:Bacterial Outer Membrane Proteins; D029941:Fish Proteins