title: The reasons of Trithorax-like expression disturbance in Trl3609allele of Drosophila melanogaster.
abstract: The regulatory region of the Trl gene was analyzed using the mutation Trl3609, resulting from the insertion of the P-element into the promoter region of the gene as well as mutations obtained on its basis. It is shown that two last transcription start sites, which are most often used in vitro in S2 cells, are almost not used in vivo. Experimental data indicate that transcription terminators in transposons play an important role in the decrease in the transcription level of the recipient gene.
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: D A
authors.initials: DA
authors.lastname: Karagodin
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Battulina
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia.
authors.forename: T I
authors.initials: TI
authors.lastname: Merkulova
authors.affiliation: Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences, Novosibirsk, 630090, Russia. barich@bionet.nsc.ru.
authors.forename: E M
authors.initials: EM
authors.lastname: Baricheva
mesh_terms: D000273:Adipose Tissue / Q000378:metabolism; D000483:Alleles; D000818:Animals; D015152:Blotting, Northern; D002460:Cell Line; D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins / Q000235:genetics* / Q000378:metabolism*; D029721:Drosophila Proteins / Q000235:genetics* / Q000378:metabolism*; D004331:Drosophila melanogaster; D017952:Ganglia, Invertebrate / Q000378:metabolism; D005786:Gene Expression Regulation; D005821:Genetic Techniques; D060227:Imaginal Discs / Q000378:metabolism; D007814:Larva; D016254:Mutagenesis, Insertional; D011401:Promoter Regions, Genetic; D012469:Salivary Glands / Q000378:metabolism; D014157:Transcription Factors / Q000235:genetics* / Q000378:metabolism*
chemical_list: D004251:DNA Transposable Elements; D004268:DNA-Binding Proteins; D029721:Drosophila Proteins; D014157:Transcription Factors; C063460:Trl protein, Drosophila