title: [Structural studies of chromatin remodeling factors].
abstract: Changes of chromatin structure require participation of chromatin remodeling factors (CRFs), which are ATP-dependent multisubunit complexes that change the structure of the nucleosome without covalently modifying its components. CRFs act together with other protein factors to regulate the extent of chromatin condensation. Four CRF families are currently distinguished based on their structural and biochemical characteristics: SWI/SNF, ISWI, Mi-2/CHD, and SWR/INO80. X-ray diffraction analysis and electron microscopy are the main methods to obtain structural information about macromolecules. CRFs are difficult to obtain in crystal because of their large sizes and structural heterogeneity, and transmission electron microscopy (TEM) is mostly employed in their structural studies. The review considers all structures obtained for CRFs by TEM and discusses several models of CRF-nucleosome interactions.
authors.affiliation: Biological Faculty, Moscow State University, Moscow, 119234 Russia.
authors.forename: O I
authors.initials: OI
authors.lastname: Volokh
authors.affiliation: Biochemistry Department, Evdokimov University of Medicine and Dentistry, Moscow, 127473 Russia.
authors.forename: N I
authors.initials: NI
authors.lastname: Derkacheva
authors.affiliation: Biological Faculty, Moscow State University, Moscow, 119234 Russia.|Fox Chase Cancer Center, Philadelphia, PA, 19111 USA.
authors.forename: V M
authors.initials: VM
authors.lastname: Studitsky
authors.affiliation: Biological Faculty, Moscow State University, Moscow, 119234 Russia.|sokolova184@gmail.com.
authors.forename: O S
authors.initials: OS
authors.lastname: Sokolova
mesh_terms: D000074183:ATPases Associated with Diverse Cellular Activities; D000251:Adenosine Triphosphatases / Q000737:chemistry* / Q000378:metabolism; D000818:Animals; D002843:Chromatin / Q000737:chemistry*; D042002:Chromatin Assembly and Disassembly; D002868:Chromosomal Proteins, Non-Histone / Q000737:chemistry* / Q000378:metabolism; D018360:Crystallography, X-Ray / Q000379:methods; D004265:DNA Helicases / Q000737:chemistry* / Q000378:metabolism; D004268:DNA-Binding Proteins; D006801:Humans; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex / Q000737:chemistry*; D046529:Microscopy, Electron, Transmission / Q000379:methods; D020836:Protein Structure, Quaternary; D014157:Transcription Factors / Q000737:chemistry* / Q000378:metabolism
chemical_list: D002843:Chromatin; D002868:Chromosomal Proteins, Non-Histone; D004268:DNA-Binding Proteins; C086468:ISWI protein; C445179:SWI-SNF-B chromatin-remodeling complex; D014157:Transcription Factors; D056245:Mi-2 Nucleosome Remodeling and Deacetylase Complex; D000251:Adenosine Triphosphatases; D000074183:ATPases Associated with Diverse Cellular Activities; D004265:DNA Helicases; C524421:INO80 protein, human
keywords: chromatin; chromatin remodeling factors; nucleosome; transcription; transmission electron microscopy