title: NADPH oxidase activation played a critical role in the oxidative stress process in stable coronary artery disease.
abstract: OBJECTIVE
  The study was designed to investigate the oxidative stress levels of endothelial progenitor cells (EPCs) in stable coronary artery disease (CAD) and to explore the underlying mechanisms of NADPH oxidase activation and subsequent EPCs dysfunction.
  
  
  METHODS
  EPCs were isolated from patients with stable CAD (n=50) and matched healthy volunteers (n=50). NADPH oxidase activation was detected by measuring the expression of each subunit using western blotting and qPCR analyses and the membrane translocation of p47phoxusing immunofluorescence. The in vivo angiogenesis capacity was evaluated using immunofluorescence by transplanting EPCs into a rat hind limb ischemia model. The PKC inhibitor GÖ-6983 was used to determine the role of PKC in NADPH oxidase activation.
  
  
  RESULTS
  Oxidative stress level was increased and the in vivo angiogenesis capacity was impaired in EPCs obtained from CAD subjects with the activation of NADPH oxidase. P47phoxmembrane translocation increased in CAD group vs controls. These effects were resolved by NADPH oxidase inhibition. Up-regulation of PKCα/β2 was found in EPCs from CAD subjects, PKC inhibition GÖ-6983 could reduce the expression and activity of NADPH oxidation.
  
  
  CONCLUSIONS
  NADPH oxidase activation via p47phoxmembrane translocation played a critical role in the initiation and progression of CAD, and the PKCα/β2 signaling pathway might be involved.
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityZhejiang, China; Biomedical Research (Therapy) Center, Sir Run Run Shaw HospitalHangzhou, Zhejiang, China.
authors.forename: Jiefang
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital Hangzhou, Zhejiang, China.
authors.forename: Meihui
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University Zhejiang, China.
authors.forename: Zhengwei
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University Zhejiang, China.
authors.forename: Xukun
authors.initials: X
authors.lastname: Bi
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang University Zhejiang, China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Song
authors.affiliation: Biomedical Research (Therapy) Center, Sir Run Run Shaw Hospital Hangzhou, Zhejiang, China.
authors.forename: Shaoxiang
authors.initials: S
authors.lastname: Weng
authors.affiliation: Department of Cardiology, Sir Run Run Shaw Hospital, College of Medicine, Zhejiang UniversityZhejiang, China; Biomedical Research (Therapy) Center, Sir Run Run Shaw HospitalHangzhou, Zhejiang, China.
authors.forename: Guosheng
authors.initials: G
authors.lastname: Fu
keywords: NADPH oxidase; PKC; endothelial progenitor cells; reactive oxygen species; stable coronary artery disease