title: Glutamicibacter halophytocola sp. nov., an endophytic actinomycete isolated from the roots of a coastal halophyte, Limonium sinense.
abstract: A novel actinobacterium, designated KLBMP 5180T, was isolated from the surface-sterilized root of a coastal halophyte, Limonium sinense, collected from the city of Lianyungang, Jiangsu Province, eastern China. The isolate was Gram-stain-positive, aerobic and non-motile. The components of the cell-wall peptidoglycan were lysine, glutamic acid and alanine. The predominant menaquinone was MK-9. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phospholipid, one unidentified glycolipid and two unidentified lipids. anteiso-C15 : 0 and iso-C16 : 0 were the major cellular fatty acids. The DNA G+C content of strain KLBMP 5180T was 60.0 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KLBMP 5180T belongs to the genus Glutamicibacter and was related most closely to Glutamicibacter nicotianae DSM 20123T (99.3 % similarity), Glutamicibacterarilaitensis Re117T (99.3 %) and Glutamicibacter mysorens LMG 16219T (99.1 %); similarity to other type strains of the genus Glutamicibacter was lower than 98.5 %. However, DNA-DNA relatedness values between strain KLBMP 5180T, G . nicotianae DSM 20123T, G. arilaitensis Re117T and G. mysorens LMG 16219T were 47.5±2.6, 51.3±3.1 and 41.2±4.3 %, respectively. The combination of DNA-DNA hybridization, phylogenetic, phenotypic and chemotaxonomic data supported the suggestion that strain KLBMP 5180T represents a novel species of the genus Glutamicibacter, for which the name Glutamicibacterhalophytocola sp. nov. is proposed. The type strain is KLBMP 5180T (=DSM 101718T=KCTC 39692T).
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Wei-Wei
authors.initials: WW
authors.lastname: Feng
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Tian-Tian
authors.initials: TT
authors.lastname: Wang
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Juan-Luan
authors.initials: JL
authors.lastname: Bai
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ding
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Ke
authors.initials: K
authors.lastname: Xing
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Ji-Hong
authors.initials: JH
authors.lastname: Jiang
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Xue
authors.initials: X
authors.lastname: Peng
authors.affiliation: The Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, PR China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Qin
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D002681:China; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D008835:Micrococcaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D009693:Nucleic Acid Hybridization; D010457:Peptidoglycan / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D018517:Plant Roots / Q000382:microbiology*; D029606:Plumbaginaceae / Q000382:microbiology*; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D055051:Salt-Tolerant Plants / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010457:Peptidoglycan; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9