title: miRNA-144 induces microglial autophagy and inflammation following intracerebral hemorrhage.
abstract: Autophagic activation mediated inflammation contributes to brain injury of intracerebral hemorrhage (ICH). MiRNAs play a key role in inflammation, which negatively and posttranscriptionally regulate gene expression and function. Modulating the mTOR signal, a central regulator of autophagy, could be of great significance for ICH. However, the specific of miRNA is unknown. In the current study, we detected the miRNA-144 expression, autophagic activity and inflammation of microglia in ICH. We also knocked down endogenous miRNA-144 to regulate autophagy and inflammation in ICH. In addition, we assessed the neurological damge in ICH mice. We found that ICH promoted miRNA-144 expression but downregulated mTOR expression. In addition, upregulation of mTOR attenuated microglial autophagy and inflammation in ICH. Furthermore, downregulation of miRNA-144 also inhibited inflammation, brain edema and improved neurological functions in ICH mice. Taken together, our findings suggested that miRNA-144 was a crucial regulator of autophagy via regulation of mTOR, and represented a promising therapeutical strategy for ICH.
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
authors.forename: Anyong
authors.initials: A
authors.lastname: Yu
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
authors.forename: Tianxi
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
authors.forename: Wenyi
authors.initials: W
authors.lastname: Zhong
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
authors.forename: Haizhen
authors.initials: H
authors.lastname: Duan
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
authors.forename: Song
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Emergency, The First Affiliated Hospital of Zunyi Medical College, Guizhou 563003, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Ye
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Juan
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China.
authors.forename: Shanchuan
authors.initials: S
authors.lastname: Zhong
authors.affiliation: Department of Neurology, Yongchuan Hospital, Chongqing Medical University, Chongqing 402160, China. Electronic address: yangzhao5140@sohu.com.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Yang
mesh_terms: D000818:Animals; D001343:Autophagy / Q000235:genetics*; D002543:Cerebral Hemorrhage / Q000150:complications* / Q000209:etiology / Q000378:metabolism / Q000473:pathology; D015870:Gene Expression; D007249:Inflammation / Q000209:etiology* / Q000378:metabolism / Q000473:pathology; D051379:Mice; D035683:MicroRNAs / Q000235:genetics*; D017628:Microglia / Q000378:metabolism* / Q000473:pathology / Q000648:ultrastructure; D058570:TOR Serine-Threonine Kinases / Q000378:metabolism
chemical_list: C551064:MIRN144 microRNA, mouse; D035683:MicroRNAs; D058570:TOR Serine-Threonine Kinases
keywords: Autophagy; ICH; Inflammation; Microglia; miRNA-144