title: Synthetic Fluorophores for Visualizing Biomolecules in Living Systems.
abstract: The last decade has witnessed significant advance in the imaging of living systems using fluorescent markers. This progress has been primarily associated with the discovery of different spectral variants of fluorescent proteins. However, the fluorescent protein technology has its own limitations and, in some cases, the use of low-molecular-weight fluorophores is preferable. In this review, we describe the arsenal of synthetic fluorescent tools that are currently in researchers' hands and span virtually the entire spectrum, from the UV to visible and, further, to the near-infrared region. An overview of recent advances in site-directed introduction of synthetic fluorophores into target cellular objects is provided. Application of these fluorescent probes to the solution of a wide range of biological problems, in particular, to the determination of local ion concentrations and pH in living systems, is discussed.
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St., 16/10, Moscow, 117997, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Martynov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St., 16/10, Moscow, 117997, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Pakhomov
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St., 16/10, Moscow, 117997, Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Popova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St., 16/10, Moscow, 117997, Russia.
authors.forename: I E
authors.initials: IE
authors.lastname: Deyev
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya St., 16/10, Moscow, 117997, Russia.
authors.forename: A G
authors.initials: AG
authors.lastname: Petrenko
keywords: fluorescence microscopy; fluorophore; measurement of ion concentration; measurement of local pH; site-directed reaction