title: Assessment of phospholipid synthesis related biomarkers for perinatal asphyxia: a piglet study.
abstract: The prompt and reliable identification of infants at risk of hypoxic-ischemic encephalopathy secondary to perinatal asphyxia in the first critical hours is important for clinical decision-making and yet still remains a challenge. This work strives for the evaluation of a panel of metabolic biomarkers that have been associated with the hypoxic-ischemic insult in the perinatal period. Plasma and urine samples from a consolidated newborn piglet model of hypoxia and withdrawn before and at different time points after a hypoxic insult were analyzed and compared to a control group. Time-dependent metabolic biomarker profiles were studied and observed patterns were similar to those of lactate levels, which are currently considered the gold standard for assessing hypoxia. Class prediction performance could be improved by the use of a combination of the whole panel of determined metabolites in plasma as compared to lactate values. Using a multivariate model including lactate together with the studied metabolic biomarkers allowed to improve the prediction performance of duration of hypoxia time, which correlates with the degree of brain damage. The present study evidences the usefulness of choline and related metabolites for improving the early assessment of the severity of the hypoxic insult.
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
authors.forename: Ángel
authors.initials: Á
authors.lastname: Sánchez-Illana
authors.affiliation: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Sognsvannsveien 20, 0372 Oslo, Norway.|Pediatric Department, Vestfold Hospital Trust, Halfdan Wilhelmsens allé 17, 3103 Tønsberg, Norway.
authors.forename: Rønnaug
authors.initials: R
authors.lastname: Solberg
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Lliso
authors.affiliation: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Sognsvannsveien 20, 0372 Oslo, Norway.
authors.forename: Leonid
authors.initials: L
authors.lastname: Pankratov
authors.affiliation: Human &Environmental Health &Safety (HEHS), Leitat Technological Center, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.|Unidad Analítica, Health Research Institute La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Quintás
authors.affiliation: Department of Pediatric Research, Institute for Surgical Research, University of Oslo, Oslo University Hospital - Rikshospitalet, Sognsvannsveien 20, 0372 Oslo, Norway.
authors.forename: Ola Didrik
authors.initials: OD
authors.lastname: Saugstad
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.|Division of Neonatology, University &Polytechnic Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
authors.forename: Máximo
authors.initials: M
authors.lastname: Vento
authors.affiliation: Neonatal Research Group, Health Research Institute Hospital La Fe, Avenida Fernando Abril Martorell 106, 46026 Valencia, Spain.
authors.forename: Julia
authors.initials: J
authors.lastname: Kuligowski
mesh_terms: D000818:Animals; D000831:Animals, Newborn; D001238:Asphyxia Neonatorum / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D015415:Biomarkers / Q000097:blood / Q000652:urine; D002794:Choline; D005260:Female; D006801:Humans; D002534:Hypoxia, Brain / Q000378:metabolism / Q000503:physiopathology; D020925:Hypoxia-Ischemia, Brain / Q000097:blood / Q000503:physiopathology* / Q000652:urine; D019344:Lactic Acid / Q000378:metabolism*; D011247:Pregnancy; D012720:Severity of Illness Index; D013552:Swine
chemical_list: D015415:Biomarkers; D019344:Lactic Acid; D002794:Choline