title: Effects of cranberry extracts on gene expression in THP-1 cells.
abstract: Cranberry contains high levels of nutrients and bioactive molecules that have health-promoting properties. The purpose of the present studies was to determine if cranberry extracts (CEs) contain phytochemicals that exert anti-inflammatory effects. The human monocytic cell line THP-1 was treated with two CEs (CE and 90MX) and subsequently challenged with Lipopolysaccharides (LPS). Tumor necrosis factorα(TNFα) expression was decreased in the CE-treated cells, indicative of an anti-inflammatory effect. Gene expression microarrays identified several immune-related genes that were responsive to CEs including interferon-induced protein with tetratricopeptide repeats 1 and 3 (IFIT 1 and 3), macrophage scavenger receptor 1 (MSR1) and colony-stimulating factor 2 (CSF2). In addition, in the CE-treated cells, metallothionein 1F and other metal-responsive genes were induced. Taken together, this data indicates that CEs contain bioactive components that have anti-inflammatory effects and may protect cells from oxidative damage.
authors.affiliation: Department of Veterinary and Biomedical Sciences and Center for Molecular Toxicology and Carcinogenesis Penn State University 325 Life Sciences Building University Park Pennsylvania 16802.
authors.forename: Daniel B
authors.initials: DB
authors.lastname: Hannon
authors.affiliation: Department of Veterinary and Biomedical Sciences and Center for Molecular Toxicology and Carcinogenesis Penn State University 325 Life Sciences Building University Park Pennsylvania 16802.
authors.forename: Jerry T
authors.initials: JT
authors.lastname: Thompson
authors.affiliation: Ocean Spray Cranberries, Inc. One Ocean Spray Drive Lakeville-Middleboro Massachusetts 02349.
authors.forename: Christina
authors.initials: C
authors.lastname: Khoo
authors.affiliation: UnitedBio-Med Inc. 102 Hunters Run Dobbs Ferry New York 10502.
authors.forename: Vijaya
authors.initials: V
authors.lastname: Juturu
authors.affiliation: Department of Veterinary and Biomedical Sciences and Center for Molecular Toxicology and Carcinogenesis Penn State University 325 Life Sciences Building University Park Pennsylvania 16802; INDIGO Biosciences Inc.1981 Pine Hall Road State College Pennsylvania 16801.
authors.forename: John P
authors.initials: JP
authors.lastname: Vanden Heuvel
keywords: Gene expression; macrophage; nutrigenomics; proanthocyanidins; reactive oxygen species