title: In vivo feasibility test using transparent carbon nanotube-coated polydimethylsiloxane sheet at brain tissue and sciatic nerve.
abstract: Carbon nanotubes, with their unique and outstanding properties, such as strong mechanical strength and high electrical conductivity, have become very popular for the repair of tissues, particularly for those requiring electrical stimuli. Polydimethylsiloxane (PDMS)-based elastomers have been used in a wide range of biomedical applications because of their optical transparency, physiological inertness, blood compatibility, non-toxicity, and gas permeability. In present study, most of artificial nerve guidance conduits (ANGCs) are not transparent. It is hard to confirm the position of two stumps of damaged nerve during nerve surgery and the conduits must be cut open again to observe regenerative nerves after surgery. Thus, a novel preparation method was utilized to produce a transparent sheet using PDMS and multiwalled carbon nanotubes (MWNTs) via printing transfer method. Characterization of the PDMS/MWNT (PM) sheets revealed their unique physicochemical properties, such as superior mechanical strength, a certain degree of electrical conductivity, and high transparency. Characterization of the in vitro and in vivo usability was evaluated. PM sheets showed high biocompatibility and adhesive ability. In vivo feasibility tests of rat brain tissue and sciatic nerve revealed the high transparency of PM sheets, suggesting that it can be used in the further development of ANGCs. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 1736-1745, 2017.
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
authors.forename: Caifeng
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
authors.forename: Sangjin
authors.initials: S
authors.lastname: Oh
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea.
authors.forename: Hyun Ah
authors.initials: HA
authors.lastname: Lee
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
authors.forename: Jieun
authors.initials: J
authors.lastname: Kang
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
authors.forename: Ki-Jae
authors.initials: KJ
authors.lastname: Jeong
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
authors.forename: Seon Woo
authors.initials: SW
authors.lastname: Kang
authors.affiliation: Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Republic of Korea.
authors.forename: Dae Youn
authors.initials: DY
authors.lastname: Hwang
authors.affiliation: Department of Cogno-Mechatronics Engineering, Pusan National University, Busan, 46241, Republic of Korea.
authors.forename: Jaebeom
authors.initials: J
authors.lastname: Lee
mesh_terms: D000818:Animals; D001921:Brain / Q000502:physiology*; D002448:Cell Adhesion; D002460:Cell Line; D002470:Cell Survival / Q000187:drug effects; D020099:Coated Materials, Biocompatible / Q000737:chemistry* / Q000633:toxicity; D004129:Dimethylpolysiloxanes / Q000737:chemistry* / Q000633:toxicity; D048091:Guided Tissue Regeneration / Q000379:methods*; D006801:Humans; D008297:Male; D037742:Nanotubes, Carbon / Q000737:chemistry* / Q000633:toxicity; D009416:Nerve Regeneration; D017207:Rats, Sprague-Dawley; D012584:Sciatic Nerve / Q000502:physiology*
chemical_list: D020099:Coated Materials, Biocompatible; D004129:Dimethylpolysiloxanes; D037742:Nanotubes, Carbon; C013830:baysilon
keywords: carbon nanotube; cell adhesion; neuronal cell culture; polydimethylsiloxane; transparent sheet