title: Biological characteristic effects of human dental pulp stem cells on poly-ε-caprolactone-biphasic calcium phosphate fabricated scaffolds using modified melt stretching and multilayer deposition.
abstract: Craniofacial bone defects such as alveolar cleft affect the esthetics and functions that need bone reconstruction. The advanced techniques of biomaterials combined with stem cells have been a challenging role for maxillofacial surgeons and scientists. PCL-coated biphasic calcium phosphate (PCL-BCP) scaffolds were created with the modified melt stretching and multilayer deposition (mMSMD) technique and merged with human dental pulp stem cells (hDPSCs) to fulfill the component of tissue engineering for bone substitution. In the present study, the objective was to test the biocompatibility and biofunctionalities that included cell proliferation, cell viability, alkaline phosphatase activity, osteocalcin, alizarin red staining for mineralization, and histological analysis. The results showed that mMSMD PCL-BCP scaffolds were suitable for hDPSCs viability since the cells attached and spread onto the scaffold. Furthermore, the constructs of induced hDPSCs and scaffolds performed ALP activity and produced osteocalcin and mineralized nodules. The results indicated that mMSMD PCL-BCP scaffolds with hDPSCs showed promise in bone regeneration for treatment of osseous defects.
authors.affiliation: Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand.
authors.forename: Natkrita
authors.initials: N
authors.lastname: Wongsupa
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand. drthongchai@hotmail.com.
authors.forename: Thongchai
authors.initials: T
authors.lastname: Nuntanaranont
authors.affiliation: Department of Preventive Dentistry, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand.
authors.forename: Suttatip
authors.initials: S
authors.lastname: Kamolmattayakul
authors.affiliation: Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Prince of Songkla University, Hat Yai, 90112, Songkhla, Thailand.
authors.forename: Nuttawut
authors.initials: N
authors.lastname: Thuaksuban
mesh_terms: D000293:Adolescent; D000328:Adult; D000469:Alkaline Phosphatase / Q000737:chemistry; D001672:Biocompatible Materials / Q000737:chemistry*; D001861:Bone Regeneration / Q000187:drug effects*; D002130:Calcium Phosphates / Q000737:chemistry; D002460:Cell Line; D049109:Cell Proliferation; D002470:Cell Survival; D003782:Dental Pulp / Q000166:cytology* / Q000187:drug effects; D006801:Humans; D016130:Immunophenotyping; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D015675:Osteocalcin / Q000737:chemistry; D011091:Polyesters / Q000737:chemistry; D013234:Stem Cells / Q000166:cytology / Q000187:drug effects*; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry*; D055815:Young Adult
chemical_list: D001672:Biocompatible Materials; D002130:Calcium Phosphates; D011091:Polyesters; D015675:Osteocalcin; C016240:polycaprolactone; C020243:calcium phosphate; D000469:Alkaline Phosphatase