title: Piceatannol exhibits anti-inflammatory effects on macrophages interacting with adipocytes.
abstract: Piceatannol (PIC), a natural analog of resveratrol (RES), is a phytochemical found in passion fruit seeds. To clarify the effects of PIC on obesity-induced inflammation in adipose tissue, we investigated the anti-inflammatory activity of PIC-related compounds (PIC, RES, and metabolites from PIC) in culture models of obese adipose tissue. Lipopolysaccharide (LPS) and conditioned medium from 3T3-L1 adipocytes (3T3-L1-CM) enhanced proinflammatory gene expression and synthesis of nitric oxide (NO), tumor necrosis factor-α(TNF-α), and interleukin-6 (IL-6) in RAW264.7 macrophages. Although each compound inhibited the mRNA expression of iNOS (inducible NO synthase), TNF-α, and IL-6, PIC potently inhibited them, and 30μmol/L PIC suppressed the LPS- and 3T3-L1-CM-induced mRNA expression of iNOS (70.4% and 69.2% suppression, respectively), TNF-α(42.6% and 47.0% suppression), and IL-6 (27.3% and 42.1% suppression). PIC also significantly suppressed production of NO (80.3% suppression) and inflammatory cytokines (TNF-α; 33.7% suppression, IL-6; 66.5% suppression). Furthermore, PIC was found to rescue the uncoupling protein 1 mRNA expression induced by isoproterenol in 10T1/2 adipocytes, which was suppressed by LPS-activated macrophages. These results suggest that PIC may attenuate the pathologic inflammation triggered by adipose tissues.
authors.affiliation: Research Institute Morinaga and Company Ltd.2-1-1 Shimosueyoshi Tsurumi-ku Yokohama 230-8504 Japan; Laboratory of Molecular Functions of Food Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto 611-0011 Japan.
authors.forename: Takayuki
authors.initials: T
authors.lastname: Yamamoto
authors.affiliation: Laboratory of Molecular Functions of Food Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto 611-0011 Japan.
authors.forename: Yongjia
authors.initials: Y
authors.lastname: Li
authors.affiliation: Laboratory of Molecular Functions of Food Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto 611-0011 Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Hanafusa
authors.affiliation: Laboratory of Molecular Functions of Food Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto 611-0011 Japan.
authors.forename: Yu-Sheng
authors.initials: YS
authors.lastname: Yeh
authors.affiliation: Research Institute Morinaga and Company Ltd. 2-1-1 Shimosueyoshi Tsurumi-ku Yokohama 230-8504 Japan.
authors.forename: Hiroko
authors.initials: H
authors.lastname: Maruki-Uchida
authors.affiliation: Research Institute Morinaga and Company Ltd. 2-1-1 Shimosueyoshi Tsurumi-ku Yokohama 230-8504 Japan.
authors.forename: Shinpei
authors.initials: S
authors.lastname: Kawakami
authors.affiliation: Research Institute Morinaga and Company Ltd. 2-1-1 Shimosueyoshi Tsurumi-ku Yokohama 230-8504 Japan.
authors.forename: Masahiko
authors.initials: M
authors.lastname: Sai
authors.affiliation: Laboratory of Molecular Functions of Food Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto 611-0011 Japan; Research Unit for Physiological Chemistry Center for the Promotion of Interdisciplinary Education and Research Kyoto University Kyoto 606-8502 Japan.
authors.forename: Tsuyoshi
authors.initials: T
authors.lastname: Goto
authors.affiliation: Research Institute Morinaga and Company Ltd. 2-1-1 Shimosueyoshi Tsurumi-ku Yokohama 230-8504 Japan.
authors.forename: Tatsuhiko
authors.initials: T
authors.lastname: Ito
authors.affiliation: Laboratory of Molecular Functions of Food Division of Food Science and Biotechnology Graduate School of Agriculture Kyoto University Uji Kyoto 611-0011 Japan; Research Unit for Physiological Chemistry Center for the Promotion of Interdisciplinary Education and Research Kyoto University Kyoto 606-8502 Japan.
authors.forename: Teruo
authors.initials: T
authors.lastname: Kawada
keywords: 3T3‐L1 Adipocytes; C3H10T1/2 adipocytes; RAW264.7 macrophages; UCP1; inflammation; piceatannol