title: Evaluation of size-exclusion chromatography for the analysis of phosphorothioate oligonucleotides.
abstract: We evaluated size exclusion chromatography (SEC) for the detection of high-order structure of phosphorothioate oligonucleotides (PS-oligo). Because of strong interaction between PS-oligo and column packing material, peaks were broader and elution time was longer than those of the corresponding natural DNA oligonucleotides. However, single- and double-stranded structures of PS-oligo were clearly separated and discriminated, while single-stranded with high-order structures such as G-quadruplex and hairpin structure were not distinguished from each other.
authors.affiliation: Analytical Research Laboratories, Technology, Astellas Pharma Inc., 5-2-3 Tokodai, Tsukuba-Shi, Ibaraki 300-2698, Japan; Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan.
authors.forename: Atsuko
authors.initials: A
authors.lastname: Shimoyama
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan; Faculty of Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiori-Kita, Tondabayashi, Osaka 584-8540, Japan.
authors.forename: Aki
authors.initials: A
authors.lastname: Fujisaka
authors.affiliation: Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: obika@phs.osaka-u.ac.jp.
authors.forename: Satoshi
authors.initials: S
authors.lastname: Obika
mesh_terms: D001483:Base Sequence; D002021:Buffers; D002138:Calibration; D002850:Chromatography, Gel / Q000379:methods*; D004277:DNA, Single-Stranded / Q000032:analysis* / Q000737:chemistry; D006863:Hydrogen-Ion Concentration; D054735:Phosphorothioate Oligonucleotides / Q000032:analysis* / Q000737:chemistry
chemical_list: D002021:Buffers; D004277:DNA, Single-Stranded; D054735:Phosphorothioate Oligonucleotides
keywords: Nucleic acids therapeutics; Phosphorothioate oligonucleotides; Size exclusion chromatography