title: Haloactinomyces albus gen. nov., sp. nov., isolated from the Dead Sea.
abstract: A novel halophilic, filamentous actinomycete strain, designated AFM 10258T, was isolated from a sediment sample collected from the Dead Sea of Israel. The isolate grew with 10-35 % NaCl and did not grow without NaCl. The isolate formed white aerial mycelium and long spore chains, and two spores were separated by sterile mycelium. The spores were non-motile, spherical and rugose-surfaced. The isolate contained meso-diaminopimelic acid as the diagnostic diamino acid and galactose and arabinose as the major whole-cell sugars. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol and three unknown phospholipids. Major fatty acids were anteiso-C17 : 0, iso-C16 : 0 and iso-C15 : 0. MK-9(H4) was the predominant menaquinone and the DNA G+C content was 62.8 mol%. 16S rRNA gene sequence analysis indicated that strain AFM 10258T shared low sequence similarity with the closely related representatives of the families Pseudonocardiaceae (below 94.47 %) and Actinopolysporaceae (below 93.76 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AFM 10258T formed a robust clade with members of the family Actinopolysporaceae. On the basis of analysis of phenotypic, chemical and molecular characteristics, strain AFM 10258T is considered to represent a novel species of a new genus, for which the name Haloactinomyces albus gen. nov., sp. nov., is proposed. The type strain is AFM 10258T (=DSM 45977T=CGMCC 4.7115T).
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Hangxian
authors.initials: H
authors.lastname: Lai
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Yingying
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: Yunnan Institute of Microbiology and Life Sciences Lab Center, Yunnan University, Kunming 650091, PR China.
authors.forename: Xiu
authors.initials: X
authors.lastname: Chen
authors.affiliation: Yunnan Institute of Microbiology and Life Sciences Lab Center, Yunnan University, Kunming 650091, PR China.
authors.forename: Qinyuan
authors.initials: Q
authors.lastname: Li
authors.affiliation: Yunnan Institute of Microbiology and Life Sciences Lab Center, Yunnan University, Kunming 650091, PR China.
authors.forename: Chenglin
authors.initials: C
authors.lastname: Jiang
authors.affiliation: Yunnan Institute of Microbiology and Life Sciences Lab Center, Yunnan University, Kunming 650091, PR China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, PR China.
authors.forename: Xiaomin
authors.initials: X
authors.lastname: Wei
mesh_terms: D039903:Actinobacteria / Q000235:genetics; D000192:Actinomycetales / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D015373:Bacterial Typing Techniques; D001482:Base Composition; D004269:DNA, Bacterial / Q000235:genetics; D003960:Diaminopimelic Acid / Q000737:chemistry; D005227:Fatty Acids / Q000737:chemistry; D007557:Israel; D009792:Oceans and Seas; D010743:Phospholipids / Q000737:chemistry; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D054712:Salinity; D012623:Seawater / Q000382:microbiology*; D017422:Sequence Analysis, DNA; D024482:Vitamin K 2 / Q000031:analogs & derivatives / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D010743:Phospholipids; D012336:RNA, Ribosomal, 16S; D024482:Vitamin K 2; C073355:menaquinone 9; D003960:Diaminopimelic Acid