title: Centriolin, a centriole-appendage protein, regulates peripheral spindle migration and asymmetric division in mouse meiotic oocytes.
abstract: Unlike somatic cells mitosis, germ cell meiosis consists of 2 consecutive rounds of division that segregate homologous chromosomes and sister chromatids, respectively. The meiotic oocyte is characterized by an absence of centrioles and asymmetric division. Centriolin is a relatively novel centriolar protein that functions in mitotic cell cycle progression and cytokinesis. Here, we explored the function of centriolin in meiosis and showed that it is localized to meiotic spindles and concentrated at the spindle poles and midbody during oocyte meiotic maturation. Unexpectedly, knockdown of centriolin in oocytes with either siRNA or Morpholino micro-injection, did not affect meiotic spindle organization, cell cycle progression, or cytokinesis (as indicated by polar body emission), but led to a failure of peripheral meiotic spindle migration, large polar body emission, and 2-cell like oocytes. These data suggest that, unlike in mitotic cells, the centriolar protein centriolin does not regulate cytokinesis, but plays an important role in regulating asymmetric division of meiotic oocytes.
authors.affiliation: a Department of Animal Sciences , Chungbuk National University , Cheongju , Korea.
authors.forename: Tian-Yi
authors.initials: TY
authors.lastname: Sun
authors.affiliation: a Department of Animal Sciences , Chungbuk National University , Cheongju , Korea.
authors.forename: Hai-Yang
authors.initials: HY
authors.lastname: Wang
authors.affiliation: a Department of Animal Sciences , Chungbuk National University , Cheongju , Korea.
authors.forename: Jung-Woo
authors.initials: JW
authors.lastname: Kwon
authors.affiliation: a Department of Animal Sciences , Chungbuk National University , Cheongju , Korea.|b Department of Laboratory Animal , College of Animal Sciences, Jilin University , Changchun , China.
authors.forename: Bao
authors.identifier: http://orcid.org/0000-0003-3490-0755
authors.initials: B
authors.lastname: Yuan
authors.affiliation: a Department of Animal Sciences , Chungbuk National University , Cheongju , Korea.
authors.forename: In-Won
authors.initials: IW
authors.lastname: Lee
authors.affiliation: a Department of Animal Sciences , Chungbuk National University , Cheongju , Korea.
authors.forename: Xiang-Shun
authors.initials: XS
authors.lastname: Cui
authors.affiliation: a Department of Animal Sciences , Chungbuk National University , Cheongju , Korea.|b Department of Laboratory Animal , College of Animal Sciences, Jilin University , Changchun , China.
authors.forename: Nam-Hyung
authors.initials: NH
authors.lastname: Kim
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D002502:Centrioles / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D048749:Cytokinesis / Q000187:drug effects; D005260:Female; D015870:Gene Expression; D008540:Meiosis / Q000187:drug effects*; D051379:Mice; D008813:Mice, Inbred ICR; D015739:Nocodazole / Q000494:pharmacology; D009865:Oocytes / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D061251:Primary Cell Culture; D012333:RNA, Messenger / Q000037:antagonists & inhibitors / Q000235:genetics* / Q000378:metabolism; D034741:RNA, Small Interfering / Q000235:genetics / Q000378:metabolism; D008941:Spindle Apparatus / Q000187:drug effects / Q000378:metabolism* / Q000648:ultrastructure; D050257:Tubulin Modulators / Q000494:pharmacology
chemical_list: D018797:Cell Cycle Proteins; C000627219:Cntrl protein, mouse; D012333:RNA, Messenger; D034741:RNA, Small Interfering; D050257:Tubulin Modulators; D015739:Nocodazole
keywords: asymmetric division; centriolin; cytokinesis; meiosis; oocyte