title: Efficient biosynthesis of a Cecropin A-melittin mutant in Bacillus subtilis WB700.
abstract: The efficient production of antimicrobial peptides (AMPs) for clinical applications has attracted the attention of the scientific community. To develop a novel microbial cell factory for the efficient biosynthesis of a cecropin A-melittin mutant (CAM-W), a recombinant Bacillus subtilis WB700 expression system was genetically modified with a novel vector, including a fusion gene encoding CAM-W, the autoprotease EDDIE and the signal peptide SacB under the control of the maltose-inducible promoter Pglv. A total of 159 mg of CAM-W was obtained from 1 L of fermentation supernatant. The purified CAM-W showed a consistent size with the expected molecular weight of 3.2 kDa. Our findings suggest that this novel expression system can be used as a powerful tool for the efficient production of CAM-W.
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.|College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Shengyue
authors.initials: S
authors.lastname: Ji
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.|College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Weili
authors.initials: W
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Abdul Rasheed
authors.initials: AR
authors.lastname: Baloch
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Meng
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
authors.forename: Hengxin
authors.initials: H
authors.lastname: Li
authors.affiliation: College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, China.
authors.forename: Binyun
authors.initials: B
authors.lastname: Cao
authors.affiliation: Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, China.
authors.forename: Hongfu
authors.initials: H
authors.lastname: Zhang
mesh_terms: D023181:Antimicrobial Cationic Peptides / Q000096:biosynthesis*; D001412:Bacillus subtilis / Q000254:growth & development / Q000378:metabolism*; D008555:Melitten / Q000096:biosynthesis*; D008826:Microbial Sensitivity Tests; D050505:Mutant Proteins / Q000096:biosynthesis*; D010957:Plasmids / Q000378:metabolism; D011994:Recombinant Proteins / Q000096:biosynthesis*; D011995:Recombination, Genetic / Q000235:genetics
chemical_list: D023181:Antimicrobial Cationic Peptides; D050505:Mutant Proteins; D011994:Recombinant Proteins; D008555:Melitten; C031161:cecropin A