title: Ancestral benzo[a]pyrene exposure affects bone integrity in F3 adult fish (Oryzias latipes).
abstract: Benzo[a]pyrene (BaP) at an environmentally relevant concentration (1μg/L) has previously been shown to affect bone development in a transgenerational manner in F3 medaka (Oryzias latipes) larvae (17dph). Here, we provide novel histomorphometric data demonstrating that the impaired bone formation at an early life stage is not recoverable and can result in a persistent transgenerational impairment of bone metabolism in F3 adult fish. A decrease in bone thickness and the occurrence of microcracks in ancestrally BaP-treated adult male fish (F3) were revealed by MicroCt measurement and histopathological analysis. The expression of twenty conserved bone miRNAs were screened in medaka and their relative expression (in the F3 ancestral BaP treatment vs the F3 control fish) were determined by quantitative real-time PCR. Attempt was made to link bone miRNA expression with the potential target bone mRNA expression in medaka. Five functional pairs of mRNA/miRNA were identified (Osx/miR-214, Col2a1b/miR-29b, Runx2/miR-204, Sox9b/miR-199a-3p, APC/miR-27b). Unique knowledge of bone-related miRNA expression in medaka in response to ancestral BaP-exposure in the F3 generation is presented. From the ecological risk assessment perspective, BaP needs to be regarded as a transgenerational skeletal toxicant which exerts a far-reaching impact on fish survival and fitness. Given that the underlying mechanisms of cartilage/bone formation are conserved between medaka and mammals, the results may also shed light on the potential transgenerational effect of BaP on skeletal disorders in mammals/humans.
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
authors.forename: Frauke
authors.initials: F
authors.lastname: Seemann
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Chang-Bum
authors.initials: CB
authors.lastname: Jeong
authors.affiliation: Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.
authors.forename: Ge
authors.initials: G
authors.lastname: Zhang
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
authors.forename: Miles Teng
authors.initials: MT
authors.lastname: Wan
authors.affiliation: Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong.
authors.forename: Baosheng
authors.initials: B
authors.lastname: Guo
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Hong Kong.
authors.forename: Drew Ryan
authors.initials: DR
authors.lastname: Peterson
authors.affiliation: Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
authors.forename: Jae-Seong
authors.initials: JS
authors.lastname: Lee
authors.affiliation: State Key Laboratory in Marine Pollution, Department of Biology and Chemistry, City University of Hong Kong, Tat Chee Avenue, Hong Kong. Electronic address: bhdwtau@cityu.edu.hk.
authors.forename: Doris Wai-Ting
authors.initials: DW
authors.lastname: Au
mesh_terms: D000818:Animals; D001564:Benzo(a)pyrene / Q000633:toxicity*; D001842:Bone and Bones / Q000187:drug effects* / Q000378:metabolism; D005260:Female; D008297:Male; D035683:MicroRNAs / Q000378:metabolism; D009990:Oryzias / Q000235:genetics / Q000502:physiology*; D010012:Osteogenesis / Q000187:drug effects; D012333:RNA, Messenger / Q000378:metabolism; D014874:Water Pollutants, Chemical / Q000633:toxicity*
chemical_list: D035683:MicroRNAs; D012333:RNA, Messenger; D014874:Water Pollutants, Chemical; D001564:Benzo(a)pyrene
keywords: Epigenetics; Osteoblast activity; Osteogenic miRNAs; Osterix; Polycyclic aromatic hydrocarbons; Transgenerational inheritance