title: Identification of SSR markers closely linked to the yellow seed coat color gene in heading Chinese cabbage (Brassica rapaL. ssp.pekinensis).
abstract: Research on the yellow-seeded variety of heading Chinese cabbage will aid in broadening its germplasm resources and lay a foundation for AA genome research inBrassicacrops. Here, an F2segregating population of 1575 individuals was constructed from two inbred lines (brown-seeded '92S105' and yellow-seeded '91-125'). This population was used to identify the linkage molecular markers of the yellow seed coat trait using simple sequence repeat (SSR) techniques combined with a bulk segregant analysis (BSA). Of the 144 SSR primer pairs on the A01-A10 chromosomes from theBrassicadatabase (http://brassicadb.org/brad/), two pairs located on the A06 chromosome showed polymorphic bands between the bulk DNA pools of eight brown-seeded and eight yellow-seeded F2progeny. Based on the genome sequence, 454 SSR markers were designed to A06 to detect these polymorphic bands and were synthesized. Six SSR markers linked to the seed coat color gene were successfully selected for fine linkage genetic map construction, in which the two closest flanking markers, SSR449a and SSR317, mapped theBrsc-yegene to a 40.2 kb region with distances of 0.07 and 0.06 cM, respectively. The molecular markers obtained in this report will assist in the marker-assisted selection and breeding of yellow-seeded lines inBrassica rapaL. and other close species.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Yanjing
authors.initials: Y
authors.lastname: Ren
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Junqing
authors.initials: J
authors.lastname: Wu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
authors.forename: Lingyu
authors.initials: L
authors.lastname: Hao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Area, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China lugangzh@163.com.
authors.forename: Lugang
authors.identifier: http://orcid.org/0000-0002-3309-3640
authors.initials: L
authors.lastname: Zhang
keywords: Brassica rapa; Genetic map; SSR markers; Seed coat color