title: Nestin Expression in the Adult Mouse Retina with Pharmaceutically Induced Retinal Degeneration.
abstract: The present study investigated the temporal pattern and cellular localization of nestin in the adult mouse retina with pharmaceutically induced retinal degeneration using N-methyl-N-nitrosourea (MNU). After a single intraperitoneal injection of MNU in 8-week-old C57BL/6 mice, the animals were sacrificed at 1, 3, 5, 7, and 21 days (n = 6, in each stage). The eyes were examined by means of immunohistochemical tests using nestin, ionized calcium-binding adaptor molecule (Iba-1), CD11b, F4/80, and glial fibrillary acidic protein (GFAP). Western blot analysis and manual cell counting were performed for quantification. Nestin expression was increased after MNU administration. Nestin+/Iba-1+ cells were migrated into outer nuclear layer (ONL) and peaked at day 3 post injection (PI). Nestin+/CD11b+ cells were also mainly identified in ONL at day 3 PI and peaked at day 5. Nestin+/F4/80+ cells were shown in the subretinal space and peaked at day 3 PI. Nestin+/GFAP+ cells were distinctly increased at day 1 PI and peaked at day 5 PI. The up-regulation of nestin expression after MNU administration in adult mouse retinal microglia, and monocyte/macrophage suggests that when retinal degeneration progresses, these cells may revert to a more developmentally immature state. Müller cells also showed reactive gliosis and differentiational changes.
authors.affiliation: Department of Ophthalmology, University of Ulsan College of Medicine, Asan Medical Center, Seoul, Korea.
authors.forename: Chan Hee
authors.identifier: http://orcid.org/0000-0003-1393-0242
authors.initials: CH
authors.lastname: Moon
authors.affiliation: Department of Ophthalmology, Hanyang University Guri Hospital, Guri, Korea.
authors.forename: Heeyoon
authors.identifier: http://orcid.org/0000-0001-8744-4533
authors.initials: H
authors.lastname: Cho
authors.affiliation: Department of Ophthalmology, Soonchunhyang University Bucheon Hospital, Bucheon, Korea.
authors.forename: Yoon Kyung
authors.identifier: http://orcid.org/0000-0002-2910-6904
authors.initials: YK
authors.lastname: Kim
authors.affiliation: Department of Ophthalmology, Soonchunhyang University Bucheon Hospital, Bucheon, Korea. tkpark@schmc.ac.kr.
authors.forename: Tae Kwann
authors.identifier: http://orcid.org/0000-0001-9689-4384
authors.initials: TK
authors.lastname: Park
mesh_terms: D000818:Animals; D015153:Blotting, Western; D002135:Calcium-Binding Proteins / Q000378:metabolism; D004195:Disease Models, Animal; D005260:Female; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D007150:Immunohistochemistry; D007958:Leukocyte Count; D008770:Methylnitrosourea / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D008840:Microfilament Proteins / Q000378:metabolism; D008856:Microscopy, Fluorescence; D064231:Nestin / Q000378:metabolism*; D012160:Retina / Q000187:drug effects / Q000378:metabolism*; D012162:Retinal Degeneration / Q000139:chemically induced / Q000378:metabolism / Q000473:pathology*; D015854:Up-Regulation
chemical_list: C491049:Aif1 protein, mouse; D002135:Calcium-Binding Proteins; D005904:Glial Fibrillary Acidic Protein; D008840:Microfilament Proteins; D064231:Nestin; D008770:Methylnitrosourea
keywords: Mouse; N-methyl-N-nitrosourea (MNU); Nestin; Retinal Degeneration