title: Xuebijing injection treatment inhibits vasopermeability and reduces fluid requirements in a canine burn model.
abstract: OBJECTIVE
  High vasopermeability and excessive inflammation following severe burns may result in tissue edema, organ dysfunction and the loss of circulatory plasma volume, which can influence the doctor to do the prognosis to the patients. The study aims to examine whether Xuebijing injection (XBJ), an extracts of a traditional Chinese medicine used to treat sepsis in clinic, can reduces fluid requirements by inhibiting vasopermeability and tissue edema in a canine model after burn injury.
  
  
  METHODS
  Twenty-four beagle dogs were subjected to 50% TBSA burns, and then were randomly allocated to the following three groups: lactated Ringer's resuscitation (LR) group (n = 8), immediate LR containing Xuebijing injection (LR/XBJ) group (n = 8), and operation control group (n = 8). Hemodynamic variables and net fluid accumulation were measured. Blood samples were collected for measurement of hematocrit and circulatory plasma volume (PV). At 24 h after burn injury, heart, lung, small intestine and kidney were harvested for evaluation of the activities of myeloperoxidase (MPO) and neutrophil elastase (NE), vasopermeability, tissue water content and the amount of neutrophil infiltration.
  
  
  RESULTS
  XBJ treatment significantly reduced net fluid accumulation, and pulmonary vascular permeability index (PVPI), extravascular lung water index (ELWI), and water content of heart, small intestine, kidney and lung compared with LR group. Furthermore, XBJ infusion significantly reduced tissue activities of MPO and NE compared with LR group. The amount of neutrophil infiltration in LR/XBJ group was lower than that in LR group.
  
  
  CONCLUSIONS
  These results indicate that XBJ injection can reduce fluid requirements by inhibition of neutrophil protease-induced high vasopermeability and tissue edema.
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: F-B
authors.initials: FB
authors.lastname: Tang
authors.affiliation: Chinese PLA General Hospital, Beijing, China.|Chinese People's Armed Police Forces Academy, Hebei, China.
authors.forename: Y-L
authors.initials: YL
authors.lastname: Dai
authors.affiliation: Key Research Laboratory of Tissue Repair and Regeneration of PLA, First Hospital Affiliated to the Chinese PLA General Hospital, 100048, Beijing, China. bs0425@163.com.
authors.forename: S
authors.initials: S
authors.lastname: Hu
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: L-Q
authors.initials: LQ
authors.lastname: Ma
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: J-Y
authors.initials: JY
authors.lastname: Li
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: H-P
authors.initials: HP
authors.lastname: Zhang
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: W-H
authors.initials: WH
authors.lastname: Zhang
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: Y-G
authors.initials: YG
authors.lastname: Li
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: H-B
authors.initials: HB
authors.lastname: Wang
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: H-Y
authors.initials: HY
authors.lastname: Lin
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: Q
authors.initials: Q
authors.lastname: Hu
authors.affiliation: Chinese PLA General Hospital, Beijing, China.
authors.forename: L
authors.initials: L
authors.lastname: Li
mesh_terms: D000818:Animals; D002056:Burns / Q000188:drug therapy*; D002199:Capillary Permeability / Q000187:drug effects*; D004195:Disease Models, Animal; D004285:Dogs / Q000293:injuries*; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D005440:Fluid Therapy; D007275:Injections, Intravenous; D007552:Isotonic Solutions / Q000008:administration & dosage / Q000494:pharmacology* / Q000627:therapeutic use; D011897:Random Allocation; D012151:Resuscitation; D000077325:Ringer's Lactate
chemical_list: D004365:Drugs, Chinese Herbal; D007552:Isotonic Solutions; D000077325:Ringer's Lactate; C549376:Xuebijing
keywords: Burn injury; Canine; Fluid resuscitation; Vasopermeability; Xuebijing injection