title: A characterization of structural proteins expressed by Bombyx mori bidensovirus.
abstract: Bombyx mori bidensiovirus (BmBDV) is a species of Bidensovirus that has been was placed into a new genus within the new family Bidnaviridae by the International Committee on Taxonomy of Viruses. BmBDV causes fatal flacherie disease in silkworms, which causes large losses to the sericulture industry. BmBDV contains two sets of complementary linear single-stranded DNAs of approximately 6.5kb (viral DNA 1, VD1) and 6.0kb (viral DNA 2, VD2). VD1 and VD2 are encapsidated in separate icosahedral non-enveloped capsids, which are similar in size and shape. However, the strategies used to express BmBDV structural proteins remains unclear. In this work, a total of six structural proteins were separated by two-dimensional electrophoresis and shown to be encoded by the BmBDV VP gene via mass spectrometry. The transmission electron microscopy results showed that co-expression of the BmBDV VP and SP structural proteins in Spodoptera frugiperda sf9 cells resulted in the formation of 22-24nm virus-like particles. Furthermore, a mutation of the major structural protein-encoding VP gene, in which the second in-frame ATG codon was mutated to GCG, abrogated the production of several structural proteins, indicating that this strategy of expressing BmBDV VP is dependent on a leaky scanning translation mechanism.
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Lü
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yali
authors.initials: Y
authors.lastname: Xing
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Zhaoyang
authors.initials: Z
authors.lastname: Hu
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yanhua
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China; Laboratory Animal Research Center, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Analysis and Testing Center, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Kangmin
authors.initials: K
authors.lastname: Chen
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Feifei
authors.initials: F
authors.lastname: Zhu
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China.
authors.forename: Keping
authors.initials: K
authors.lastname: Chen
authors.affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang 212013, China. Electronic address: yaoqin@ujs.edu.cn.
authors.forename: Qin
authors.initials: Q
authors.lastname: Yao
mesh_terms: D000595:Amino Acid Sequence; D000818:Animals; D015153:Blotting, Western; D012831:Bombyx / Q000821:virology; D017990:Densovirus / Q000502:physiology*; D015180:Electrophoresis, Gel, Two-Dimensional; D046529:Microscopy, Electron, Transmission; D061987:Sf9 Cells; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; D015678:Viral Structural Proteins / Q000502:physiology* / Q000648:ultrastructure
chemical_list: D015678:Viral Structural Proteins
keywords: BmBDV; Leaky scanning translation; Structural proteins; Two-dimensional electrophoresis; Virus-like particles