title: DNA methylation assessment from human slow- and fast-twitch skeletal muscle fibers.
abstract: A new application of the reduced representation bisulfite sequencing method was developed using low-DNA input to investigate the epigenetic profile of human slow- and fast-twitch skeletal muscle fibers. Successful library construction was completed with as little as 15 ng of DNA, and high-quality sequencing data were obtained with 32 ng of DNA. Analysis identified 143,160 differentially methylated CpG sites across 14,046 genes. In both fiber types, selected genes predominantly expressed in slow or fast fibers were hypomethylated, which was supported by the RNA-sequencing analysis. These are the first fiber type-specific methylation data from human skeletal muscle and provide a unique platform for future research.NEW & NOTEWORTHYThis study validates a low-DNA input reduced representation bisulfite sequencing method for human muscle biopsy samples to investigate the methylation patterns at a fiber type-specific level. These are the first fiber type-specific methylation data reported from human skeletal muscle and thus provide initial insight into basal state differences in myosin heavy chain I and IIa muscle fibers among young, healthy men.
authors.affiliation: Human Performance Laboratory, Ball State University, Muncie, Indiana.
authors.forename: Gwénaëlle
authors.initials: G
authors.lastname: Begue
authors.affiliation: Human Performance Laboratory, Ball State University, Muncie, Indiana.
authors.forename: Ulrika
authors.initials: U
authors.lastname: Raue
authors.affiliation: Human Performance Laboratory, Ball State University, Muncie, Indiana.
authors.forename: Bozena
authors.initials: B
authors.lastname: Jemiolo
authors.affiliation: Human Performance Laboratory, Ball State University, Muncie, Indiana strappe@bsu.edu.
authors.forename: Scott
authors.initials: S
authors.lastname: Trappe
mesh_terms: D000328:Adult; D004247:DNA / Q000378:metabolism; D019175:DNA Methylation / Q000502:physiology*; D044127:Epigenesis, Genetic / Q000502:physiology; D005260:Female; D006801:Humans; D008297:Male; D018656:Muscle Fibers, Fast-Twitch / Q000378:metabolism / Q000502:physiology*; D018657:Muscle Fibers, Slow-Twitch / Q000378:metabolism / Q000502:physiology*
chemical_list: D004247:DNA
keywords: DNA methylation; RNA sequencing; low-DNA input; reduced representation bisulfite sequencing; skeletal muscle fibers