title: A bioinspired ionic liquid tagged cobalt-salophen complex for nonenzymatic detection of glucose.
abstract: The development of efficient and cost effective nonenzymatic biosensors with remarkable sensitivity, selectivity and stability for the detection of biomolecules, especially glucose is one of the major challenges in materials- and electrochemistry. Herein, we report the design and preparation of nonenzymatic biosensor based on an ionic liquid tagged cobalt-salophen metal complex (Co-salophen-IL) immobilized on electrochemically reduced graphene oxide (ERGO) for the detection of glucose via an electrochemical oxidation. The bioinspired Co-salophen-IL complex has been synthesized and immobilized on ERGO, which was previously deposited on a screen printed carbon electrode (SPE) to form the Co-salophen-IL/ERGO/SPE nonenzymatic biosensor. The electrochemical behaviour of this modified electrode was studied using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Notably, the Co-salophen-IL/ERGO/SPE biosensor exhibited excellent electrocatalytic activity towards glucose oxidation in 0.1M NaOH, based on which an amperometric sensor has been developed. The modified electrode has shown prominent performance towards glucose detection over a wide linear range from 0.2µM to 1.8mM with a detection limit and sensitivity of 0.79µM and 62µAmM-1respectively. The detection was carried out at 0.40V and such a less working potential excludes the interference from the coexisting oxidizable analytes. The role of Co-salophen, IL and ERGO in the electrocatalytic activity has been systematically investigated. Furthermore, the biosensor demonstrated high stability with good reproducibility.
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Michael
authors.initials: M
authors.lastname: Benjamin
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Devaraj
authors.initials: D
authors.lastname: Manoj
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Kathavarayan
authors.initials: K
authors.lastname: Thenmozhi
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India.
authors.forename: Pundlik Rambhau
authors.initials: PR
authors.lastname: Bhagat
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India. Electronic address: duraisarav@gmail.com.
authors.forename: Duraisamy
authors.initials: D
authors.lastname: Saravanakumar
authors.affiliation: Department of Chemistry, School of Advanced Sciences, VIT University, Vellore 632014, India. Electronic address: senthilanalytical@gmail.com.
authors.forename: Sellappan
authors.initials: S
authors.lastname: Senthilkumar
mesh_terms: D002384:Catalysis; D003035:Cobalt / Q000737:chemistry*; D055664:Electrochemical Techniques / Q000379:methods*; D005947:Glucose / Q000032:analysis*; D006108:Graphite / Q000737:chemistry*; D052578:Ionic Liquids / Q000737:chemistry*; D057230:Limit of Detection; D010084:Oxidation-Reduction; D010087:Oxides / Q000737:chemistry; D015203:Reproducibility of Results; D012459:Salicylates / Q000737:chemistry*
chemical_list: D052578:Ionic Liquids; D010087:Oxides; D012459:Salicylates; C090416:salophen; D003035:Cobalt; D006108:Graphite; D005947:Glucose
keywords: Bioinspired complex; Glucose; Graphene oxide; Ionic liquid; Nonenzymatic biosensor; Salophen