title: Isolating DNA from Gram-Negative Bacteria.
abstract: The isolation of DNA from bacteria, described in this protocol, relies upon the use of sodium dodecyl sulfate and proteinase K to lyse the cells. High-molecular-weight DNA is then sheared (to reduce its viscosity and make it more manageable), extracted with phenol:chloroform, and precipitated with isopropanol. DNA isolated according to this procedure ranges from 30 to 80 kb in length.
authors.forename: Michael R
authors.initials: MR
authors.lastname: Green
authors.forename: Joseph
authors.initials: J
authors.lastname: Sambrook
mesh_terms: D019840:2-Propanol; D001433:Bacteriolysis; D002725:Chloroform / Q000378:metabolism; D004269:DNA, Bacterial / Q000235:genetics / Q000302:isolation & purification*; D003902:Detergents / Q000378:metabolism; D019286:Endopeptidase K / Q000378:metabolism; D006090:Gram-Negative Bacteria / Q000737:chemistry* / Q000235:genetics*; D008967:Molecular Biology / Q000379:methods*; D019800:Phenol / Q000378:metabolism; D012967:Sodium Dodecyl Sulfate / Q000378:metabolism; D012997:Solvents / Q000378:metabolism
chemical_list: D004269:DNA, Bacterial; D003902:Detergents; D012997:Solvents; D019800:Phenol; D012967:Sodium Dodecyl Sulfate; D002725:Chloroform; D019286:Endopeptidase K; D019840:2-Propanol