title: Altered mucosal expression of microRNAs in pediatric patients with inflammatory bowel disease.
abstract: BACKGROUND
  MicroRNAs (miRs) came recently into focus as promising novel research targets offering new insights into the pathogenesis of inflammatory bowel diseases (IBD).
  
  
  OBJECTIVE
  The aim of our study was to identify a pediatric IBD (pIBD) characteristic miR profile serving as potential Crohn's disease (CD) and ulcerative colitis (UC) specific diagnostic pattern and to further analyze the related target genes.
  
  
  METHODS
  Small RNA sequencing was performed on inflamed and intact colonic biopsies of CD, and control patients. Selected miRs were further investigated by RT-PCR, complemented with an UC group, in order to address the differential diagnostic potential of miRs in the two IBD subtypes. To analyze network connection of differentially expressed miRs and their target genes MiRTarBase database and previous transcriptome sequencing data from pediatric patient groups were used.
  
  
  RESULTS
  Sequencing analysis identified 170 miRs with altered expression. RT-PCR analysis revealed altered expression of miR-31, -125a, -142-3p, and -146a discriminating between the inflamed mucosa of CD and UC. In the intact mucosa of CD patients the expression of miR-18a, -20a, -21, -31, -99a, -99b, -100, -125a, -126, -142-5p, -146a, -185, -204, -221, and -223 was elevated compared to the controls. The expression of miR-20a, -204 and -221 was elevated exclusively in the intact region of CD patients compared to the controls. Enrichment analysis identified main IBD-related functional groups.
  
  
  CONCLUSIONS
  We demonstrated a characteristic colonic miR pattern in pIBD that could facilitate deeper understanding of the pathomechanism of IBD and may serve as a diagnostic tool.
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: Nóra Judit
authors.initials: NJ
authors.lastname: Béres
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: Zoltán
authors.initials: Z
authors.lastname: Kiss
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: Zsófia
authors.initials: Z
authors.lastname: Sztupinszki
authors.affiliation: MTA-SE Tumor Progression Research Group, Budapest, Hungary.
authors.forename: Gábor
authors.initials: G
authors.lastname: Lendvai
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: András
authors.initials: A
authors.lastname: Arató
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary; MTA-SE Pediatrics and Nephrology Research Group, Budapest, Hungary.
authors.forename: Erna
authors.initials: E
authors.lastname: Sziksz
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary; MTA-SE Pediatrics and Nephrology Research Group, Budapest, Hungary.
authors.forename: Ádám
authors.initials: Á
authors.lastname: Vannay
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary; MTA-SE Pediatrics and Nephrology Research Group, Budapest, Hungary.
authors.forename: Attila J
authors.initials: AJ
authors.lastname: Szabó
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: Katalin Eszter
authors.initials: KE
authors.lastname: Müller
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: Áron
authors.initials: Á
authors.lastname: Cseh
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary.
authors.forename: Kriszta
authors.initials: K
authors.lastname: Boros
authors.affiliation: 1st Department of Pediatrics, Semmelweis University, Budapest, Hungary; MTA-SE Pediatrics and Nephrology Research Group, Budapest, Hungary. Electronic address: veres.gabor@med.semmelweis-univ.hu.
authors.forename: Gábor
authors.initials: G
authors.lastname: Veres
mesh_terms: D000293:Adolescent; D002648:Child; D003106:Colon / Q000473:pathology*; D005260:Female; D020869:Gene Expression Profiling; D005786:Gene Expression Regulation; D005819:Genetic Markers; D020022:Genetic Predisposition to Disease; D006801:Humans; D006814:Hungary; D015212:Inflammatory Bowel Diseases / Q000175:diagnosis* / Q000235:genetics* / Q000473:pathology; D007413:Intestinal Mucosa / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D010372:Pediatrics
chemical_list: D005819:Genetic Markers; D035683:MicroRNAs
keywords: Inflammatory bowel disease; MicroRNA; Mucosal; Pediatric