title: Description of a new Neoactinomyxum type actinosporean from the oligochaete Branchiura sowerbyi Beddard.
abstract: Actinosporean infection of oligochaetes living in the mud of a commercial gibel carp pond with myxosporean disease was studied. Six actinospore types were detected exclusively from the oligochaete Branchiura sowerbyi Beddard with very high prevalence (18%). Five out of the six types were identified as the same actinosporeans described in previous reports, the sixth actinosporean was identified as a new Neoactinomyxum type and described here based on morphological and molecular characterisation. Spore body of the actinospore was globular, much smaller than caudal processes. Three caudal processes were disc-like in apical view, hemispherical in side view, closer together and encircling the spore body. The number of sporoplasm cells was detected as eight in one specimen. The new actinosporean markedly differed from other Neoactinomyxum types in literature having much bigger caudal processes. DNA sequence analyses further confirmed the morphological identification, and revealed the actinosporean described here (KU641392) possessed less than 94% sequence similarity with myxozoans available in the GenBank database.
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China. xibw@ffrc.cn.
authors.forename: Bing Wen
authors.initials: BW
authors.lastname: Xi
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.|Wuxi Fishery College, Nanjing Agricultural University, Wuxi, 210095, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Li
authors.affiliation: Nanyang Medical College, Nanyang, 473061, China.
authors.forename: Qing Chun
authors.initials: QC
authors.lastname: Liu
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.
authors.forename: Kai
authors.initials: K
authors.lastname: Chen
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China.|Wuxi Fishery College, Nanjing Agricultural University, Wuxi, 210095, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Teng
authors.affiliation: Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi, 214081, China. xiej@ffrc.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Xie
mesh_terms: D000818:Animals; D055764:Myxozoa / Q000145:classification* / Q000166:cytology / Q000235:genetics; D009835:Oligochaeta / Q000469:parasitology*; D012337:RNA, Ribosomal, 18S / Q000235:genetics; D013045:Species Specificity; D033761:Spores, Protozoan / Q000166:cytology
chemical_list: D012337:RNA, Ribosomal, 18S