title: Protective Effect of SFE-CO2of Ligusticum chuanxiong Hort Against d-Galactose-Induced Injury in the Mouse Liver and Kidney.
abstract: This study was designed to explore how supercritical fluid CO2extract of Ligusticum chuanxiong Hort (CX) protects mouse liver and kidney from d-galactose-induced injury. The antioxidant capacity of CX was confirmed both in vitro and in vivo. The d-galactose-induced malondialdehyde increase was attenuated by CX, as well as the increase in aspartate aminotransferase, alanine aminotransferase, blood urea nitrogen, and creatinine level. In addition, the activities of antioxidant enzymes were markedly renewed, and the gene expressions of these enzymes were upregulated in CX groups. The results of histological analysis suggested that CX could effectively attenuate the d-galactose-induced structure damage. Furthermore, results of Western blotting analysis showed that CX significantly inhibited the upregulation of nuclear factor protein expression caused by d-galactose. In conclusion, CX could attenuate the liver and kidney injury in d-galactose-treated mice, and the mechanism might be associated with attenuating oxidative stress and inflammatory response.
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .|2 School of Chinese Medicine, The Chinese University of Hong Kong , Shatin, N.T., Hong Kong SAR, China .
authors.forename: Zhi-Zhun
authors.initials: ZZ
authors.lastname: Mo
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .
authors.forename: Yu-Hong
authors.initials: YH
authors.lastname: Liu
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .
authors.forename: Cai-Lan
authors.initials: CL
authors.lastname: Li
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .
authors.forename: Lie-Qiang
authors.initials: LQ
authors.lastname: Xu
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .
authors.forename: Ling-Ling
authors.initials: LL
authors.lastname: Wen
authors.affiliation: 2 School of Chinese Medicine, The Chinese University of Hong Kong , Shatin, N.T., Hong Kong SAR, China .
authors.forename: Yan-Fang
authors.initials: YF
authors.lastname: Xian
authors.affiliation: 2 School of Chinese Medicine, The Chinese University of Hong Kong , Shatin, N.T., Hong Kong SAR, China .
authors.forename: Zhi-Xiu
authors.initials: ZX
authors.lastname: Lin
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .
authors.forename: Janis Ya-Xian
authors.initials: JY
authors.lastname: Zhan
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .
authors.forename: Jian-Nan
authors.initials: JN
authors.lastname: Chen
authors.affiliation: 3 The Second Affiliated Hospital of Guangzhou University of Chinese Medicine , Guangdong Provincial Academy of Chinese Medical Sciences, Guangzhou, P.R. China .
authors.forename: Fang-Fang
authors.initials: FF
authors.lastname: Xu
authors.affiliation: 1 Guangdong Provincial Key Laboratory of New Chinese Medicinals Development and Research, Guangzhou University of Chinese Medicine , Guangzhou, P.R. China .|4 Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine , Dongguan, P.R. China .
authors.forename: Zi-Ren
authors.initials: ZR
authors.lastname: Su
mesh_terms: D000375:Aging / Q000473:pathology; D000410:Alanine Transaminase / Q000097:blood; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology; D001219:Aspartate Aminotransferases / Q000097:blood; D001806:Blood Urea Nitrogen; D001835:Body Weight / Q000187:drug effects; D002245:Carbon Dioxide / Q000737:chemistry*; D025924:Chromatography, Supercritical Fluid; D003404:Creatinine / Q000097:blood; D051546:Cyclooxygenase 2 / Q000378:metabolism; D005690:Galactose; D008401:Gas Chromatography-Mass Spectrometry; D005786:Gene Expression Regulation / Q000187:drug effects; D007249:Inflammation / Q000473:pathology; D020128:Inhibitory Concentration 50; D007668:Kidney / Q000187:drug effects / Q000201:enzymology / Q000293:injuries* / Q000473:pathology; D030004:Ligusticum / Q000737:chemistry*; D015227:Lipid Peroxidation / Q000187:drug effects; D008099:Liver / Q000187:drug effects / Q000201:enzymology / Q000293:injuries* / Q000473:pathology; D008297:Male; D051379:Mice; D016328:NF-kappa B / Q000378:metabolism; D052247:Nitric Oxide Synthase Type II / Q000378:metabolism; D009928:Organ Specificity; D010936:Plant Extracts / Q000737:chemistry / Q000494:pharmacology*; D020011:Protective Agents / Q000494:pharmacology*
chemical_list: D000975:Antioxidants; D016328:NF-kappa B; D010936:Plant Extracts; D020011:Protective Agents; D002245:Carbon Dioxide; D003404:Creatinine; D052247:Nitric Oxide Synthase Type II; D051546:Cyclooxygenase 2; D001219:Aspartate Aminotransferases; D000410:Alanine Transaminase; D005690:Galactose
keywords: Ligusticum chuanxiong; d-galactose; inflammatory response; oxidative stress