title: Synthesis of magnetic zwitterionic-hydrophilic material for the selective enrichment of N-linked glycopeptides.
abstract: In consideration of the close connection between glycopeptides and human diseases, the efficient method to separate and enrich glycopeptides from complex biological samples is urgently required. In the work, we developed a magnetic zwitterionic-hydrophilic material for highly effective separation and analysis of glycopeptides from complex samples. The Fe3O4particles were covered with a thick layer of polymer by one-step reflux-precipitation polymerization (RPP), subsequently decorated by Au nanoparticles (Au NPs) through in situ reduction and finally modified with zwitterionic groups. The abundant zwitterionic sites facilitate the selective enrichment of glycopeptides. Besides, the prepared Fe3O4@PGMA@Au-l-cys showed high detection sensitivity (5 fmol IgG digest), approving enrichment capacity (75mgg-1), satisfactory enrichment recovery (89.8%), and great performance in the analysis and profiling of low-abundance N-linked glycopeptides. Furthermore, the prepared material was employed in the enrichment of glycopeptides in intricate biological samples, and 774 unique N-glycosylation sites from 411 N-glycosylated proteins were reliably identified in three replicate analyses of a 75μg protein sample extracted from mouse liver, suggesting wide application prospect in glycoproteomics.
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
authors.forename: Yiman
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
authors.forename: Yajing
authors.initials: Y
authors.lastname: Chen
authors.affiliation: State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai, 200050, PR China.
authors.forename: Zhichao
authors.initials: Z
authors.lastname: Xiong
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
authors.forename: Xudong
authors.initials: X
authors.lastname: Sun
authors.affiliation: Fudan University, Shanghai, 200433, PR China.
authors.forename: Quanqing
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
authors.forename: Yangyang
authors.initials: Y
authors.lastname: Gan
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China. Electronic address: zhanglingyi@ecust.edu.cn.
authors.forename: Lingyi
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China. Electronic address: weibingzhang@ecust.edu.cn.
authors.forename: Weibing
authors.initials: W
authors.lastname: Zhang
mesh_terms: D000818:Animals; D006020:Glycopeptides / Q000737:chemistry* / Q000302:isolation & purification*; D006031:Glycosylation; D057927:Hydrophobic and Hydrophilic Interactions; D008099:Liver / Q000737:chemistry; D008280:Magnetics / Q000379:methods*; D053768:Metal Nanoparticles / Q000737:chemistry; D051379:Mice; D058105:Polymerization; D011108:Polymers / Q000138:chemical synthesis / Q000737:chemistry; D040901:Proteomics; D012834:Silver / Q000737:chemistry
chemical_list: D006020:Glycopeptides; D011108:Polymers; D012834:Silver
keywords: Enrichment; Glycopeptides; Magnetic nanoparticles; Mass spectrum; Zwitterionic-hydrophilic interaction liquid chromatography