title: Recent progress in Bacillus subtilis spore-surface display: concept, progress, and future.
abstract: With the increased knowledge on spore structure and advances in biotechnology engineering, the newly developed spore-surface display system confers several inherent advantages over other microbial cell-surface display systems including enhanced stability and high safety. Bacillus subtilis is the most commonly used Bacillus species for spore-surface display. The expression of heterologous antigen or protein on the surface of B. subtilis spores has now been practiced for over a decade with noteworthy success. As an update and supplement to other previous reviews, we comprehensively summarize recent studies in the B. subtilis spore-surface display technique. We focus on its benefits as well as the critical factors affecting its display efficiency and offer suggestions for the future success of this field.
authors.affiliation: Jiyang College, Zhejiang Agriculture and Forestry University, Zhuji, Zhejiang, 311800, China. wh2989@zafu.edu.cn.
authors.forename: He
authors.initials: H
authors.lastname: Wang
authors.affiliation: Jiyang College, Zhejiang Agriculture and Forestry University, Zhuji, Zhejiang, 311800, China.
authors.forename: Yunxiang
authors.initials: Y
authors.lastname: Wang
authors.affiliation: School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China.
authors.forename: Ruijin
authors.initials: R
authors.lastname: Yang
mesh_terms: D001412:Bacillus subtilis / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D001426:Bacterial Proteins / Q000235:genetics / Q000378:metabolism; D001709:Biotechnology / Q000379:methods*; D061505:Cell Surface Display Techniques; D008565:Membrane Proteins / Q000235:genetics / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000378:metabolism*; D013171:Spores, Bacterial / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008565:Membrane Proteins; D011993:Recombinant Fusion Proteins
keywords: Application; Bacillus subtilis; Display efficiency; Factor; Spore; Surface display