title: Live visualization of genomic loci with BiFC-TALE.
abstract: Tracking the dynamics of genomic loci is important for understanding the mechanisms of fundamental intracellular processes. However, fluorescent labeling and imaging of such loci in live cells have been challenging. One of the major reasons is the low signal-to-background ratio (SBR) of images mainly caused by the background fluorescence from diffuse full-length fluorescent proteins (FPs) in the living nucleus, hampering the application of live cell genomic labeling methods. Here, combining bimolecular fluorescence complementation (BiFC) and transcription activator-like effector (TALE) technologies, we developed a novel method for labeling genomic loci (BiFC-TALE), which largely reduces the background fluorescence level. Using BiFC-TALE, we demonstrated a significantly improved SBR by imaging telomeres and centromeres in living cells in comparison with the methods using full-length FP.
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Center for Quantitative Biology, Peking University, Beijing 100871, China.
authors.forename: Huan
authors.initials: H
authors.lastname: Hu
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.|Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China.|Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, China.|Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China.|State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Hongmin
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China.
authors.forename: Miao
authors.initials: M
authors.lastname: Ding
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Center for Quantitative Biology, Peking University, Beijing 100871, China.
authors.forename: Hui
authors.initials: H
authors.lastname: An
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China.
authors.forename: Yingping
authors.initials: Y
authors.lastname: Hou
authors.affiliation: Center for Quantitative Biology, Peking University, Beijing 100871, China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.|Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China.|Beijing Advanced Innovation Center for Genomics (ICG), Peking University, Beijing 100871, China.|State Key Laboratory of Protein and Plant Gene Research, Peking University, Beijing 100871, People's Republic of China.
authors.forename: Wensheng
authors.initials: W
authors.lastname: Wei
authors.affiliation: School of Life Sciences, Peking University, Beijing 100871, China.|Biodynamic Optical Imaging Center (BIOPIC), Peking University, Beijing 100871, China.
authors.forename: Yujie
authors.initials: Y
authors.lastname: Sun
authors.affiliation: Center for Quantitative Biology, Peking University, Beijing 100871, China.|Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Tang
mesh_terms: D002460:Cell Line; D056426:Genetic Loci; D006801:Humans; D000069416:Intravital Microscopy / Q000379:methods*; D008856:Microscopy, Fluorescence / Q000379:methods*; D013194:Staining and Labeling / Q000379:methods*