title: Remission effect of vitamin C on isoflurane-induced apoptosis and its mechanism.
abstract: This study aims to discuss the remission effect of vitamin C on isoflurane-induced apoptosis of rats and its possible mechanism of action, to provide a theoretical basis for postoperative cognitive impairment. Reactive oxygen species (ROS) detection, adenosine triphosphate (ATP) test, MTT method and Morris water maze were applied for detection tests. For data statistics, double factor analysis of variance (ANOVA) and post hoc Bonferroni test were adopted. It was found that vitamin C could slow down the isoflurane-induced accumulation of ROS in H4-APP cells; moreover, it could relieve the activation of caspase-3 and increase cell survival rate to inhibit the occurrence of apoptosis, indicating that ROS was the source of cell toxicity. On the other hand, vitamin C could protect the cells with its antioxidant effect. It was proved that vitamin C could remit isoflurane-induced apoptosis and relieve the decline in learning and memory ability of rats.
authors.affiliation: Anesthesia Department, Shandong Jiaotong hospital, Jinan, Shandong, China.
authors.forename: J
authors.initials: J
authors.lastname: Hu
authors.affiliation: Anesthesia Department, Shandong Jiaotong hospital, Jinan, Shandong, China.
authors.forename: B F
authors.initials: BF
authors.lastname: Gao
authors.affiliation: Anesthesia Department, Shandong Jiaotong hospital, Jinan, Shandong, China.
authors.forename: H Q
authors.initials: HQ
authors.lastname: Li
authors.affiliation: Anesthesia Department, Shandong Jiaotong hospital, Jinan, Shandong, China.
authors.forename: Z Z
authors.initials: ZZ
authors.lastname: Zhang
authors.affiliation: Anesthesia Department, Shandong Jiaotong hospital, Jinan, Shandong, China.
authors.forename: Y L
authors.initials: YL
authors.lastname: Lei
authors.affiliation: Anesthesia Department, Shandong Jiaotong hospital, Jinan, Shandong, China.
authors.forename: G Q
authors.initials: GQ
authors.lastname: Sun
authors.affiliation: Shandong University Shool of Public Health, Jinan, Shandong, China.
authors.forename: D M
authors.initials: DM
authors.lastname: Guo
mesh_terms: D018685:Anesthetics, Inhalation / Q000633:toxicity*; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001205:Ascorbic Acid / Q000494:pharmacology*; D015153:Blotting, Western; D001921:Brain / Q000187:drug effects; D003072:Cognition Disorders / Q000139:chemically induced*; D004195:Disease Models, Animal; D006801:Humans; D007530:Isoflurane / Q000633:toxicity*; D018782:Maze Learning / Q000187:drug effects; D009474:Neurons / Q000187:drug effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D018685:Anesthetics, Inhalation; D000975:Antioxidants; D017382:Reactive Oxygen Species; D007530:Isoflurane; D001205:Ascorbic Acid