title: Changes in liver proteins of rats fed standard and high-fat and sucrose diets induced by fish omega-3 PUFAs and their combination with grape polyphenols according to quantitative proteomics.
abstract: This study considered the physiological modulation of liver proteins due to the supplementation with fish oils under two dietary backgrounds: standard or high in fat and sucrose (HFHS), and their combination with grape polyphenols. By using a quantitative proteomics approach, we showed that the capacity of the supplements for regulating proteins depended on the diet; namely, 10 different proteins changed into standard diets, while 45 changed into the HFHS diets and only scarcely proteins were found altered in common. However, in both contexts, fish oils were the main regulatory force, although the addition of polyphenols was able to modulate some fish oils' effects. Moreover, we demonstrated the ability of fish oils and their combination with grape polyphenols in improving biochemical parameters and reducing lipogenesis and glycolysis enzymes, enhancing fatty acid beta-oxidation and insulin signaling and ameliorating endoplasmic reticulum stress and protein oxidation when they are included in an unhealthy diet.
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC), E-36208 Vigo, Spain. Electronic address: luciamendez@iim.csic.es.
authors.forename: Lucía
authors.initials: L
authors.lastname: Méndez
authors.affiliation: Unidad de Proteómica, Centro Nacional de Biotecnología (CNB-CSIC), E-28049 Madrid, Spain.
authors.forename: Sergio
authors.initials: S
authors.lastname: Ciordia
authors.affiliation: Unidad de Proteómica, Centro Nacional de Biotecnología (CNB-CSIC), E-28049 Madrid, Spain.
authors.forename: María Soledad
authors.initials: MS
authors.lastname: Fernández
authors.affiliation: Unidad de Proteómica, Centro Nacional de Biotecnología (CNB-CSIC), E-28049 Madrid, Spain.
authors.forename: Silvia
authors.initials: S
authors.lastname: Juárez
authors.affiliation: Unidad de Proteómica, Centro Nacional de Biotecnología (CNB-CSIC), E-28049 Madrid, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Ramos
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC), E-36208 Vigo, Spain.
authors.forename: Manuel
authors.initials: M
authors.lastname: Pazos
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC), E-36208 Vigo, Spain.
authors.forename: José M
authors.initials: JM
authors.lastname: Gallardo
authors.affiliation: Instituto de Química Avanzada de Cataluña (IQAC-CSIC), E-08034 Barcelona, Spain.
authors.forename: Josep Lluís
authors.initials: JL
authors.lastname: Torres
authors.affiliation: Unidad de Farmacología, Facultad de Medicina y Ciencias de la Salud, Universidad Rovira i Virgili, E-43201 Reus, Spain.
authors.forename: M Rosa
authors.initials: MR
authors.lastname: Nogués
authors.affiliation: Instituto de Investigaciones Marinas (IIM-CSIC), E-36208 Vigo, Spain.
authors.forename: Isabel
authors.initials: I
authors.lastname: Medina
mesh_terms: D000818:Animals; D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000627:therapeutic use; D000975:Antioxidants / Q000627:therapeutic use; D000070601:Diet, Carbohydrate Loading / Q000009:adverse effects; D059305:Diet, High-Fat / Q000009:adverse effects; D019422:Dietary Sucrose / Q000009:adverse effects; D019587:Dietary Supplements; D059865:Endoplasmic Reticulum Stress; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D005260:Female; D005395:Fish Oils / Q000627:therapeutic use*; D005786:Gene Expression Regulation; D056604:Grape Seed Extract / Q000627:therapeutic use*; D007333:Insulin Resistance; D008099:Liver / Q000378:metabolism*; D065626:Non-alcoholic Fatty Liver Disease / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism; D059808:Polyphenols / Q000627:therapeutic use; D040901:Proteomics / Q000379:methods; D011897:Random Allocation; D011921:Rats, Inbred WKY
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D000975:Antioxidants; D019422:Dietary Sucrose; D015525:Fatty Acids, Omega-3; D005395:Fish Oils; D056604:Grape Seed Extract; D059808:Polyphenols
keywords: Bioactive compounds; Fish oils; Grape polyphenols; High-fat, high-sucrose diet; Liver protein regulation; Wistar Kyoto rat