title: Hyaluronic acid-coated chitosan nanoparticles induce ROS-mediated tumor cell apoptosis and enhance antitumor efficiency by targeted drug delivery via CD44.
abstract: BACKGROUND
  A targeted drug nanoparticle (NP) delivery system has shown potential as a possible cancer treatment. Given its merits, such as its selective distribution at tumor sites and its controllable drug release, drug-loaded NPs can be effectively delivered to selected organs and targeted cells, thus enhancing its antitumor efficiency and reducing its toxicity.
  
  
  METHODS
  We reported that hyaluronic acid (HA)-coated chitosan NPs promoted the drug delivery of 5-fluorouracil (5-Fu) into tumor cells that highly expressed CD44.
  
  
  RESULTS
  Our new findings suggested that HA-coated chitosan NPs enhanced drug accumulation by effectively transporting NPs into CD44-overexpressed tumor cells, and they also resulted in mitochondrial damage induced by the production of reactive oxygen species (ROS). Compared to free drug and uncoated NPs, HA-coated chitosan NPs exhibited stronger inhibition rates and induced obvious apoptosis in CD44-overexpressed A549 cells.
  
  
  CONCLUSIONS
  Biocompatible and biodegradable HA-coated chitosan NPs were developed to encapsulate a chemotherapeutic drug (5-Fu) to enhance drug accumulation in tumor cells and to improve the agent's antitumor efficiency by offering targeted drug delivery via CD44.
authors.affiliation: School of Pharmacy, Jinzhou Medical University, Jinzhou, 121000, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Wang
authors.affiliation: School of Pharmacy, Jinzhou Medical University, Jinzhou, 121000, People's Republic of China.
authors.forename: Jiahui
authors.initials: J
authors.lastname: Hou
authors.affiliation: School of Veterinary Medicine, Jinzhou Medical University, Jinzhou, 121000, People's Republic of China.
authors.forename: Chang
authors.initials: C
authors.lastname: Su
authors.affiliation: School of Pharmacy, Jinzhou Medical University, Jinzhou, 121000, People's Republic of China. liangzhao79@163.com.
authors.forename: Liang
authors.initials: L
authors.lastname: Zhao
authors.affiliation: School of Pharmacy, Jinzhou Medical University, Jinzhou, 121000, People's Republic of China. shiyijie119@163.com.
authors.forename: Yijie
authors.initials: Y
authors.lastname: Shi
mesh_terms: D000072283:A549 Cells; D000970:Antineoplastic Agents / Q000494:pharmacology; D017209:Apoptosis / Q000187:drug effects; D048271:Chitosan / Q000737:chemistry*; D016503:Drug Delivery Systems; D065546:Drug Liberation; D059865:Endoplasmic Reticulum Stress; D005472:Fluorouracil / Q000494:pharmacology; D056945:Hep G2 Cells; D006801:Humans; D018960:Hyaluronan Receptors / Q000378:metabolism*; D006820:Hyaluronic Acid / Q000737:chemistry*; D008928:Mitochondria / Q000378:metabolism; D053758:Nanoparticles / Q000737:chemistry*; D010316:Particle Size; D017382:Reactive Oxygen Species / Q000378:metabolism
chemical_list: D000970:Antineoplastic Agents; C497483:CD44 protein, human; D018960:Hyaluronan Receptors; D017382:Reactive Oxygen Species; D006820:Hyaluronic Acid; D048271:Chitosan; D005472:Fluorouracil
keywords: CD44; Hyaluronic acid; Mitochondria; Nanoparticles; Reactive oxygen species