title: Neurobrucellosis: Unexpected Answer From Metagenomic Next-Generation Sequencing.
abstract: A diagnosis of brucellosis can be difficult because routine culture and serological methods exhibit variable sensitivity and specificity. We present the use of a metagenomic next- generation sequencing assay to diagnose a case of neurobrucellosis from cerebrospinal fluid, resulting in the institution of appropriate antibiotic treatment and a favorable clinical outcome.
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases.
authors.forename: Kanokporn
authors.initials: K
authors.lastname: Mongkolrattanothai
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles and Keck School of Medicine, University of Southern California, Los Angeles.|Department of Laboratory Medicine.|UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California.
authors.forename: Samia N
authors.initials: SN
authors.lastname: Naccache
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Bender
authors.affiliation: Department of Laboratory Medicine.|UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California.
authors.forename: Erik
authors.initials: E
authors.lastname: Samayoa
authors.affiliation: Department of Laboratory Medicine.|UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California.
authors.forename: Elizabeth
authors.initials: E
authors.lastname: Pham
authors.affiliation: Department of Laboratory Medicine.|UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California.
authors.forename: Guixia
authors.initials: G
authors.lastname: Yu
authors.affiliation: Department of Pathology and Laboratory Medicine, Children's Hospital Los Angeles and Keck School of Medicine, University of Southern California, Los Angeles.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Dien Bard
authors.affiliation: Department of Laboratory Medicine.|UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California.
authors.forename: Steve
authors.initials: S
authors.lastname: Miller
authors.affiliation: Department of Pediatrics, Division of Infectious Diseases.|Department of Pediatrics, Division of Infectious Diseases, University of California, Los Angeles.
authors.forename: Grace
authors.initials: G
authors.lastname: Aldrovandi
authors.affiliation: Department of Laboratory Medicine.|Department of Medicine, Division of Infectious Diseases, University of California, San Francisco.|UCSF-Abbott Viral Diagnostics and Discovery Center, San Francisco, California.
authors.forename: Charles Y
authors.initials: CY
authors.lastname: Chiu
mesh_terms: D002002:Brucella / Q000235:genetics; D002006:Brucellosis / Q000134:cerebrospinal fluid / Q000175:diagnosis*; D002648:Child; D005260:Female; D059014:High-Throughput Nucleotide Sequencing / Q000379:methods*; D006801:Humans; D008279:Magnetic Resonance Imaging; D056186:Metagenomics / Q000379:methods; D013116:Spinal Cord / Q000000981:diagnostic imaging
keywords: diagnostic microbiology; encephalitis; meningitis; metagenomic next-generation sequencing; neurobrucellosis