title: Porcine parvovirus capsid protein expressed in Escherichia coli self-assembles into virus-like particles with high immunogenicity in mice and guinea pigs.
abstract: Porcine parvovirus (PPV) is a causative agent of reproductive failure in pregnant sows. Classical inactivated vaccine is extensively used to control PPV infection, but problems concerning safety, such as incomplete inactivation may occur. In this study, a novel subunit vaccine against PPV based on virus-like particles (VLPs) formed from the complete PPV VP2 protein expressed in a prokaryotic system with co-expressed chaperones is reported. The VLPs have a similar size, shape, and hemagglutination property to the PPV. Immunization with these VLPs stimulated the neutralization antibody and hemagglutination inhibition (HI) antibody responses in mice and guinea pigs. The lymphocyte proliferation response and cytokine secretion was also induced in immunized guinea pigs comparable to those immunized with PPV inactivated vaccine. In addition, immunization with VLPs also significantly reduced the PPV content in the spleen of guinea pigs 14 days after the challenge with intact virus. These studies suggest that PPV VLPs created as described here could be a potential candidate for vaccine development.
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China; Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
authors.forename: Pengchao
authors.initials: P
authors.lastname: Ji
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
authors.forename: Yunchao
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
authors.forename: Yumei
authors.initials: Y
authors.lastname: Chen
authors.affiliation: School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China.
authors.forename: Aiping
authors.initials: A
authors.lastname: Wang
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Dawei
authors.initials: D
authors.lastname: Jiang
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Baolei
authors.initials: B
authors.lastname: Zhao
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China.
authors.forename: Jvcai
authors.initials: J
authors.lastname: Wang
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China.
authors.forename: Shujun
authors.initials: S
authors.lastname: Chai
authors.affiliation: College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, China.
authors.forename: Enmin
authors.initials: E
authors.lastname: Zhou
authors.affiliation: Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, 450002, China; School of Life Sciences, Zhengzhou University, Zhengzhou, 450001, China; College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China. Electronic address: zhanggaiping2003@163.com.
authors.forename: Gaiping
authors.initials: G
authors.lastname: Zhang
mesh_terms: D000818:Animals; D057134:Antibodies, Neutralizing / Q000097:blood; D000956:Antigens, Viral / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism*; D036022:Capsid Proteins / Q000008:administration & dosage / Q000235:genetics* / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism; D004926:Escherichia coli / Q000235:genetics; D006168:Guinea Pigs; D006385:Hemagglutination Inhibition Tests; D000071497:Immunogenicity, Vaccine; D008213:Lymphocyte Activation; D051379:Mice; D028323:Parvovirus, Porcine / Q000737:chemistry; D013154:Spleen / Q000821:virology; D013552:Swine; D022223:Vaccines, Subunit / Q000008:administration & dosage / Q000235:genetics / Q000276:immunology; D058425:Vaccines, Virus-Like Particle / Q000008:administration & dosage / Q000737:chemistry / Q000235:genetics / Q000276:immunology*; D019065:Virus Assembly
chemical_list: D057134:Antibodies, Neutralizing; D000956:Antigens, Viral; D036022:Capsid Proteins; D016207:Cytokines; C528774:VP2 protein, porcine parvovirus; D022223:Vaccines, Subunit; D058425:Vaccines, Virus-Like Particle
keywords: Escherichia coli; Immunogenicity; Porcine parvovirus; Virus like particle