title: Ortho-phthalic acid esters in lipophilic extract from the cell culture of Aconitum baicalense Turcz ex Rapaics 1907.
abstract: Optically active bis-2R(-)ethylhexyl o-phthalate was obtained with 0.18% yield from dry cultured cells of Aconitum baicalense Turcz ex Rapaics 1907 by extraction with petroleum ether followed by silica gel column chromatography. Its structure was confirmed by the analysis of13C and1H NMR spectra. Seasonal fluctuations of quantitative phthalate content in A. baicalense cells were identified. The tests were performed under conditions excluding the presence of phthalates in reagents, materials, and laboratory dishes. The same substance was shown to be produced by cultivated cells of other plants. Biosynthesis of esters of ortho-phthalic acid by cultivated plant cells was discovered for the first time.
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia.|Irkutsk National Research Technical University, ul. Lermontova 89, Irkutsk, 664074, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Semenov
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia. enikeev@sifibr.irk.ru.
authors.forename: A G
authors.initials: AG
authors.lastname: Enikeev
authors.affiliation: Irkutsk State University, Irkutsk, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Snetkova
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia.
authors.forename: A V
authors.initials: AV
authors.lastname: Permyakov
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Sokolova
authors.affiliation: Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences, ul. Lermontova 132, Irkutsk, 664033, Russia.
authors.forename: L V
authors.initials: LV
authors.lastname: Dudareva
mesh_terms: D031841:Aconitum / Q000737:chemistry*; D000473:Alkanes / Q000737:chemistry; D066241:Carbon-13 Magnetic Resonance Spectroscopy; D002478:Cells, Cultured; D002850:Chromatography, Gel; D003993:Dibutyl Phthalate / Q000737:chemistry / Q000302:isolation & purification; D004224:Diterpenes; D005612:Freeze Drying; D008401:Gas Chromatography-Mass Spectrometry; D006586:Hexanes / Q000737:chemistry; D007211:Indoles; D010795:Phthalic Acids / Q000737:chemistry* / Q000302:isolation & purification; D010936:Plant Extracts / Q000737:chemistry* / Q000302:isolation & purification; D066244:Proton Magnetic Resonance Spectroscopy; D012822:Silicon Dioxide; D012997:Solvents / Q000737:chemistry
chemical_list: D000473:Alkanes; D004224:Diterpenes; D006586:Hexanes; D007211:Indoles; D010795:Phthalic Acids; D010936:Plant Extracts; D012997:Solvents; C510263:sespendole; D003993:Dibutyl Phthalate; D012822:Silicon Dioxide; C004544:naphtha; C007379:diethyl phthalate