title: Production of enzymes by a newly isolated Bacillus sp. TMF-1 in solid state fermentation on agricultural by-products: The evaluation of substrate pretreatment methods.
abstract: Study on potential of different agro-industrial waste residues for supporting the growth of newly isolated Bacillus sp. TMF-1 strain under solid-state fermentation (SSF) was conducted aiming to produce several industrially valuable enzymes. Since the feasibility of the initial study was confirmed, further objectives included evaluation of several pretreatments of the studied agricultural by-products (soybean meal, sunflower meal, maize bran, maize pericarp, olive oil cake and wheat bran) on the microbial productivity as means of enhancing the yields of produced proteases, α-amylases, cellulases and pectinases. Among acid/alkaline treatment, ultrasound and microwave assisted methods, chemical treatments superiorly affected most of the studied substrates. Highest yields of produced proteases (50.5IUg-1) and α-amylases (50.75IUg-1) were achieved on alkaline treated corn pericarp. Alkaline treatment also promoted the secretion of cellulases on maize bran (1.19IUg-1). Highest yield of pectinases was obtained on untreated soybean meal (64.90IUg-1).
authors.affiliation: Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Abdalla Ali
authors.initials: AA
authors.lastname: Salim
authors.affiliation: Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia. Electronic address: sgrbavcic@tmf.bg.ac.rs.
authors.forename: Sanja
authors.initials: S
authors.lastname: Grbavčić
authors.affiliation: Innovation Center of Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Nataša
authors.initials: N
authors.lastname: Šekuljica
authors.affiliation: Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Andrea
authors.initials: A
authors.lastname: Stefanović
authors.affiliation: Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Sonja
authors.initials: S
authors.lastname: Jakovetić Tanasković
authors.affiliation: Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Nevena
authors.initials: N
authors.lastname: Luković
authors.affiliation: Faculty of Technology and Metallurgy, University of Belgrade, Department of Biochemical Engineering and Biotechnology, Karnegijeva 4, 11000 Belgrade, Serbia.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Knežević-Jugović
mesh_terms: D000383:Agriculture; D001407:Bacillus / Q000254:growth & development / Q000378:metabolism*; D044602:Cellulases / Q000378:metabolism; D004043:Dietary Fiber / Q000378:metabolism; D010450:Endopeptidases / Q000378:metabolism; D005285:Fermentation; D007220:Industrial Waste; D011096:Polygalacturonase / Q000378:metabolism; D012037:Refuse Disposal / Q000379:methods*; D013025:Glycine max / Q000378:metabolism; D003313:Zea mays / Q000378:metabolism; D000516:alpha-Amylases / Q000096:biosynthesis
chemical_list: D004043:Dietary Fiber; D007220:Industrial Waste; D044602:Cellulases; D000516:alpha-Amylases; D011096:Polygalacturonase; D010450:Endopeptidases
keywords: Bacillus sp.; Cellulase; Enzyme production; Protease; Solid state fermentation