title: Immunocytochemical detection of rhamnogalacturonan II on forming cell plates in cultured tobacco cells.
abstract: Rhamnogalacturonan II (RG-II) is a region of pectin macromolecules that is present in plant primary cell walls. The RG-II region serves as the site of borate cross-linking within pectin, via which pectin macromolecules link together to form a gel. In this study, we examined whether RG-II is present in the cell plate, the precursor of primary cell walls that forms during cytokinesis. A structure inside dividing cells was labeled with a rabbit polyclonal anti-RG-II antibody and detected by immunofluorescence microscopy. An antibody against callose, a marker polysaccharide for the cell plate, also labeled the structure. In immunoelectron microscopy analyses using the anti-RG-II antibody, gold particles were distributed in electron-lucent vesicular structures that appeared to correspond to the forming cell plates in late anaphase cells. Together, these results suggest that RG-II is present in cell plates from the early phase of their assembly.
authors.affiliation: a Laboratory of Plant Nutrition, Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Ye
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: b Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Tatsuya
authors.initials: T
authors.lastname: Awano
authors.affiliation: a Laboratory of Plant Nutrition, Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Masaru
authors.initials: M
authors.lastname: Kobayashi
authors.affiliation: a Laboratory of Plant Nutrition, Division of Applied Life Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Matoh
authors.affiliation: b Laboratory of Tree Cell Biology, Division of Forest and Biomaterials Sciences , Graduate School of Agriculture, Kyoto University , Kyoto , Japan.
authors.forename: Keiji
authors.initials: K
authors.lastname: Takabe
mesh_terms: D000818:Animals; D000918:Antibody Specificity; D001692:Biological Transport; D002455:Cell Division; D002478:Cells, Cultured; D000939:Epitopes / Q000276:immunology; D007150:Immunohistochemistry; D010368:Pectins / Q000276:immunology / Q000378:metabolism*; D011817:Rabbits; D014026:Nicotiana / Q000166:cytology* / Q000378:metabolism
chemical_list: D000939:Epitopes; C042492:rhamnogalacturonan II; D010368:Pectins
keywords: Nicotiana tabacum; antibody; cell plate; pectin; rhamnogalacturonan II