title: Hapten-Specific T Cell-Mediated Skin Inflammation: Flow Cytometry Analysis of Mouse Skin Inflammatory Infiltrate.
abstract: Hapten-specific T cell-mediated skin inflammation also known as contact hypersensitivity (CHS) is characterized by a strong influx of CD8+cytotoxic T cells within the skin upon reexposure of sensitized individuals to the same hapten. As many other leukocytes are also recruited during this elicitation phase, we attempted to revisit the skin infiltrate and characterize the inflammatory pattern. Recent improvement in the isolation in conventional as well as inflammatory dendritic cell and macrophage subsets from tissues and in the use of appropriate surface markers unraveling their heterogeneity should allow to determinate their specific functions in the CHS model. Here, we describe procedures to extract those cells from the skin and to analyze them by flow cytometry using a combination of appropriate surface markers allowing further transcriptomic analysis and functional assays.
authors.affiliation: Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Nicolas
authors.initials: N
authors.lastname: Bouladoux
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France.|DBV, 92120, Montrouge, France.
authors.forename: Clotilde
authors.initials: C
authors.lastname: Hennequin
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France.
authors.forename: Camille
authors.initials: C
authors.lastname: Malosse
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France.
authors.forename: Bernard
authors.initials: B
authors.lastname: Malissen
authors.affiliation: Mucosal Immunology Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Disease, National Institutes of Health, Bethesda, MD, USA.
authors.forename: Yasmine
authors.initials: Y
authors.lastname: Belkaid
authors.affiliation: Centre d'Immunologie de Marseille-Luminy, Aix Marseille Université, Inserm, CNRS, Parc scientifique de Luminy, Case 906, 13288, Marseille Cedex 9, France. henri@ciml.univ-mrs.fr.
authors.forename: Sandrine
authors.initials: S
authors.lastname: Henri
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D015496:CD4-Positive T-Lymphocytes / Q000187:drug effects / Q000276:immunology*; D002465:Cell Movement; D003713:Dendritic Cells / Q000187:drug effects / Q000276:immunology*; D003877:Dermatitis, Contact / Q000209:etiology / Q000276:immunology* / Q000473:pathology; D004139:Dinitrofluorobenzene / Q000633:toxicity; D004423:Ear; D005434:Flow Cytometry / Q000379:methods*; D006241:Haptens / Q000633:toxicity; D051379:Mice; D008810:Mice, Inbred C57BL; D009504:Neutrophils / Q000187:drug effects / Q000276:immunology*; D012867:Skin / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D013602:T-Lymphocytes, Cytotoxic / Q000187:drug effects / Q000276:immunology*
chemical_list: D015703:Antigens, CD; D006241:Haptens; D004139:Dinitrofluorobenzene
keywords: CD4+ T cells; CD8+ T cells; Contact hypersensitivity (CHS); Dendritic cell subsets; Flow cytometry; Macrophages; Monocyte-derived cells; Neutrophils; Skin; γδ T cells