title: The nuclear RNA binding protein RBP33 influences mRNA and spliced leader RNA abundance in Trypanosoma brucei.
abstract: RNA recognition motif (RRM) containing proteins are important regulators of gene expression in trypanosomes. Here we expand our current knowledge on the exclusively nuclear localized RRM domain containing protein RBP33 of Trypanosoma brucei. Overexpression of RBP33 leads to a quick growth arrest in G2/M in bloodstream form cells likely due to an overall mRNA- and spliced leader abundance decrease while the ribosomal RNAs remain unaffected. The recombinant RBP33 binds to poly(A) and random sequence RNA in vitro confirming its role as a RNA binding protein. Finally super-resolution microscopy detects RBP33 in small punctae throughout the nucleus and surrounding the nucleolus, however the signal is depleted inside the nucleolus.
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences at the University of Bern, Switzerland.
authors.forename: Olivera
authors.initials: O
authors.lastname: Cirovic
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences at the University of Bern, Switzerland.
authors.forename: Roman
authors.initials: R
authors.lastname: Trikin
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland; Graduate School for Cellular and Biomedical Sciences at the University of Bern, Switzerland.
authors.forename: Anneliese
authors.initials: A
authors.lastname: Hoffmann
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland.
authors.forename: Nicholas
authors.initials: N
authors.lastname: Doiron
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland.
authors.forename: Martin
authors.initials: M
authors.lastname: Jakob
authors.affiliation: Institute of Cell Biology, University of Bern, Switzerland. Electronic address: torsten.ochsenreiter@izb.unibe.ch.
authors.forename: Torsten
authors.initials: T
authors.lastname: Ochsenreiter
mesh_terms: D015870:Gene Expression; D011485:Protein Binding; D015800:Protozoan Proteins / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics*; D016053:RNA, Protozoan; D020038:RNA, Spliced Leader / Q000235:genetics*; D016601:RNA-Binding Proteins / Q000235:genetics / Q000378:metabolism*; D011994:Recombinant Proteins / Q000378:metabolism; D014346:Trypanosoma brucei brucei / Q000235:genetics* / Q000378:metabolism*
chemical_list: D015800:Protozoan Proteins; D012333:RNA, Messenger; D016053:RNA, Protozoan; D020038:RNA, Spliced Leader; D016601:RNA-Binding Proteins; D011994:Recombinant Proteins
keywords: Electrophoretic mobility shift assay; RNA binding protein; Super-resolution microscopy; Trypanosoma brucei