title: Comparative study of oxidative stress parameters and acetylcholinesterase activity in the liver ofPelophylax esculentuscomplex frogs.
abstract: Comparative activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), the phase II biotransformation enzyme glutathione-S-transferase (GST), the concentrations of total glutathione (GSH), sulfhydryl groups (-SH) and the activity of the neurotoxicity biomarker acetylcholinesterase (AChE) were investigated in the livers of species belonging to thePelophylax esculentus"complex" (parental speciesPelophylax ridibundus,Pelophylax lessonae, and their hybridPelophylaxkl.esculentus) from the wetland, Obedska bara in Serbia. The condition factor (CF) and hepato somatic index (HSI) were also calculated. All three species were caught at same locality and were exposed to the same environmental conditions. Liver SOD activity was lower inP. ridibundusthan inP.kl. esculentusandP. lessonae; higher activities of CAT, GR and GST were observed inP.kl. esculentusfrogs as compared to their parental species. The activity of GSH-Px was significantly lower inP.kl. esculentus. The activity of AChE was increased inP. lessonaeas compared toP.kl. esculentusandP. ridibundus. Similar concentrations of GSH and -SH groups were observed in all investigated species.P.kl. esculentushad a higher CF, while the HSI was lower when compared to the parental species. Our findings suggest that the parental species (P. ridibundusandP. lessonae) possess more similar antioxidative responses to environmental conditions than the hybrid speciesP. kl. esculentus. The obtained results improve our understanding of the biology and physiology of these three closely related species.
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Marko
authors.initials: M
authors.lastname: Prokić
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Slavica
authors.initials: S
authors.lastname: Borković-Mitić
authors.affiliation: Faculty of Biology, Institute of Zoology, University of Belgrade, Studentski trg 16, 11000 Belgrade, Serbia.
authors.forename: Imre
authors.initials: I
authors.lastname: Krizmanić
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Jelena
authors.initials: J
authors.lastname: Gavrić
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Svetlana
authors.initials: S
authors.lastname: Despotović
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Branka
authors.initials: B
authors.lastname: Gavrilović
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Tijana
authors.initials: T
authors.lastname: Radovanović
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Slađan
authors.initials: S
authors.lastname: Pavlović
authors.affiliation: Institute for Biological Research ''Siniša Stanković'', Department of Physiology, University of Belgrade, Bulevar despota Stefana 142, 11060 Belgrade, Serbia.
authors.forename: Zorica
authors.initials: Z
authors.lastname: Saičić
keywords: Acetylcholinesterase; Antioxidative defense system; Frogs; Liver; Oxidative stress; Pelophylax esculentus complex