title: Horizontal pollen transmission of Gentian ovary ring-spot virus is initiated during penetration of the stigma and style by infected pollen tubes.
abstract: Gentian ovary ring-spot virus (GORV) infected gentian plants by pollination with GORV-infected gentian pollen grains, but the virus was not horizontally transmitted to gentian plants by transfer of pollen from GORV-infected Nicotiana benthamiana plants. However, N. benthamiana plants were infected with the virus by pollination with infected gentian pollen as well as by pollination with infected N. benthamiana pollen. When infected gentian pollen grains were placed on N. benthamiana stigmas, germinating pollen tubes penetrated into the stigmas and the styles (stigma-style). Virus infection occurred during penetration of the stigma-style, and the virus subsequently spread systemically to the mother plant. On the other hand, most infected N. benthamiana pollen grains failed to germinate on gentian stigmas, and virus infections were not detected in the stigma-style.
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan. Electronic address: isogai@iwate-u.ac.jp.
authors.forename: Masamichi
authors.initials: M
authors.lastname: Isogai
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Kamata
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
authors.forename: Syunpei
authors.initials: S
authors.lastname: Ando
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
authors.forename: Misaki
authors.initials: M
authors.lastname: Kamata
authors.affiliation: Plant Pathology Group, Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami 024-0003, Iwate, Japan.
authors.forename: Asuka
authors.initials: A
authors.lastname: Shirakawa
authors.affiliation: Plant Pathology Group, Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami 024-0003, Iwate, Japan.
authors.forename: Ken-Taro
authors.initials: KT
authors.lastname: Sekine
authors.affiliation: Plant Pathology Laboratory, Faculty of Agriculture, Iwate University, Ueda 3-chome 18-8, Morioka 020-8550, Iwate, Japan.
authors.forename: Nobuyuki
authors.initials: N
authors.lastname: Yoshikawa
mesh_terms: D010935:Plant Diseases / Q000821:virology*; D010942:Plant Viruses / Q000472:pathogenicity*; D011058:Pollen / Q000821:virology*; D053205:Pollen Tube / Q000821:virology*; D012328:RNA Viruses / Q000472:pathogenicity*; D014026:Nicotiana / Q000821:virology*
keywords: GORV; Gentian ovary ring-spot virus; Horizontal transmission by pollen; Ovary; Pollen grain; Pollen transmission; Pollen tube; Stigma; Style