title: EXPRESSION OF E-CADHERIN AND WNT PATHWAY PROTEINS BETACATENIN, APC, TCF-4 AND SURVIVIN IN GASTRIC ADENOCARCINOMA: CLINICAL AND PATHOLOGICAL IMPLICATION.
abstract: Gastric cancer is the fifth most frequent cancer and the third most common cause of cancer-related deaths worldwide.It has been reported that Wnt/ betacatenin pathway is activated in 30-50% of these tumors. However,the deregulation of this pathway has not been fully elucidated.
  
  
  
  To determine the expression of E-cadherin, betacatenin, APC, TCF-4 and survivin proteins in gastric adenocarcinoma tissues and correlate with clinical and pathological parameters.
  
  
  
  Seventy-one patients with gastric adenocarcinoma undergoing gastrectomy were enrolled. The expression of E-cadherin, betacatenin, APC, TCF-4 and survivin proteins was detected by immunohistochemistryand related to the clinical and pathological parameters.
  
  
  
  The expression rates of E-cadherin in the membrane was 3%; betacatenin in the cytoplasm and nucleus were 23,4% and 3,1% respectively; APC in the cytoplasm was 94,6%; TCF-4 in the nucleus was 19,4%; and survivin in the nucleus 93,9%. The expression rate of E-cadherin was correlated with older patients (p=0,007), while betacatenin with tumors <5 cm (p=0,041) and APC with proximal tumors (p=0,047). Moreover, the expression of TCF-4 was significantly higher in the diffuse type (p=0,017) and T4 tumors (p=0,002).
  
  
  
  The Wnt/betacatenin is not involved in gastric carcinogenesis. However, the high frequency of survivin allows to suggest that other signaling pathways must be involved in the transformation of gastric tissue.
authors.affiliation: Postgraduate Program in Interdisciplinary Surgical Science, Federal University of São Paulo - UNIFESP, São Paulo, SP; Brazil.
authors.forename: Rodrigo Rego
authors.initials: RR
authors.lastname: Lins
authors.affiliation: Department of Pathology, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Celina Tizuko Fujiyama
authors.initials: CT
authors.lastname: Oshima
authors.affiliation: Postgraduate Program in Interdisciplinary Surgical Science, Federal University of São Paulo - UNIFESP, São Paulo, SP; Brazil.
authors.forename: Levindo Alves de
authors.initials: LA
authors.lastname: Oliveira
authors.affiliation: Department of Pathology, UNIFESP, São Paulo, SP, Brazil.
authors.forename: Marcelo Souza
authors.initials: MS
authors.lastname: Silva
authors.affiliation: Discipline of Pathology, ABC Medical School, Santo André, SP, Brazil.
authors.forename: Ana Maria Amaral Antonio
authors.initials: AM
authors.lastname: Mader
authors.affiliation: Postgraduate Program in Interdisciplinary Surgical Science, Federal University of São Paulo - UNIFESP, São Paulo, SP; Brazil.
authors.forename: Jaques
authors.initials: J
authors.lastname: Waisberg
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism*; D025601:Adenomatous Polyposis Coli Protein / Q000096:biosynthesis; D015703:Antigens, CD; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors / Q000096:biosynthesis; D015820:Cadherins / Q000096:biosynthesis*; D005260:Female; D006801:Humans; D051034:Inhibitor of Apoptosis Proteins / Q000096:biosynthesis; D008297:Male; D008875:Middle Aged; D013274:Stomach Neoplasms / Q000378:metabolism*; D000077022:Survivin; D000073940:Transcription Factor 4; D014157:Transcription Factors / Q000096:biosynthesis; D051153:Wnt Proteins / Q000096:biosynthesis*; D060449:Wnt Signaling Pathway
chemical_list: D025601:Adenomatous Polyposis Coli Protein; D015703:Antigens, CD; C107489:BIRC5 protein, human; D051778:Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; C513384:CDH1 protein, human; D015820:Cadherins; D051034:Inhibitor of Apoptosis Proteins; D000077022:Survivin; C532096:TCF4 protein, human; D000073940:Transcription Factor 4; D014157:Transcription Factors; D051153:Wnt Proteins