title: Effects of Active Components of Fuzi and Gancao Compatibility on Bax, Bcl-2, and Caspase-3 in Chronic Heart Failure Rats.
abstract: Hypaconitine (HA) and glycyrrhetinic acid (GA) are active components of Fuzi (Aconitum carmichaelii) and Gancao (Glycyrrhiza uralensisFisch); they have been used in compatibility for chronic heart failure (CHF) from ancient times. The purpose of the present research was to explore whether apoptosis pathways were related with the protective effects of HA + GA against CHF rats or not. The rats were progressed with transverse-aortic constriction (TAC) operation for 4 weeks to build the CHF state, and then the Digoxin (1 mg/kg), HA (2.07 mg/kg), GA (25 mg/kg), and HA (2.07 mg/kg) + GA (25 mg/kg) were orally administrated to rats for 1 week. The levels of BNP and cTnI in the plasma were decreased in the HA + GA group, and the heart/body weight ratio (H/B) and left ventricular (LV) parameters of transthoracic echocardiography were also declined; moreover, the expressions of Bax, Bcl-2, and caspase-3 were all improved in the HA + GA group than other groups in the immunohistochemistry and western blot methods. In general, the data suggested that Fuzi and Gancao compatibility could protect the CHF rats from apoptosis, which provided a strong evidence for further searching for mechanisms of them.
authors.affiliation: Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
authors.forename: Liqin
authors.initials: L
authors.lastname: Wang
authors.affiliation: Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: He
authors.affiliation: Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
authors.forename: Yuyan
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
authors.forename: Huifen
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
authors.forename: Jiehong
authors.identifier: 0000-0002-2758-0485
authors.initials: J
authors.lastname: Yang
authors.affiliation: Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, Zhejiang 310053, China.
authors.forename: Haitong
authors.identifier: 0000-0001-5726-7214
authors.initials: H
authors.lastname: Wan