title: Evaluation of in vitro fertilization outcomes using interleukin-8 in culture medium of human preimplantation embryos.
abstract: To investigate whether selected cytokines are detectable in the embryo culture medium (EM) of human preimplantation embryos (HPE) and what the relationship is of the cytokines with clinical outcomes.
  
  
  
  Cross-sectional study.
  
  
  
  University-affiliated tertiary teaching hospital.
  
  
  
  Three-hundred and thirty infertile women who underwent fresh cycle in vitro fertilization (IVF) between January and December 2014.
  
  
  
  Collection on the day of transfer of the EM of each embryo that was transferred in all patients for measurement of cytokine levels.
  
  
  
  Measurement of 13 selected cytokines in the EM of day-3 HPE to analyze the relationship of the cytokine with embryo quality and clinical outcome.
  
  
  
  Of the cytokines measured, only interleukin-8 (IL-8) was statistically significantly associated with clinical outcome. The rate of detectable IL-8 in the EM was 32.42%, and the pregnancy rate, implantation rate, and number of live births per in vitro fertilization (IVF) or intracytoplasmic sperm injection patient (N LBPP) were higher, and 0 IR was lower in patients for whom the medium from transferred embryos was positive for IL-8 (IL-8 positive group) compared with the patients for whom the medium tested negative for IL-8 (IL-8 negative group). Compared with the IL-8 negative group, a higher pregnancy rate was observed in the IL-8 positive group when the patients received equal good-ordinary quality embryos.
  
  
  
  In the EM from HPE, IL-8 is associated with higher pregnancy rates, higher IRs, and higher N LBPP, so IL-8 may be an independent predictor for pretransfer assessment of the embryo development potential in IVF patients.
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China. Electronic address: guanyouhuang@hotmail.com.
authors.forename: Guanyou
authors.initials: G
authors.lastname: Huang
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Congrong
authors.initials: C
authors.lastname: Zhou
authors.affiliation: Multidisciplinary Research Center, Shantou University, Shantou, People's Republic of China.
authors.forename: Chi-Ju
authors.initials: CJ
authors.lastname: Wei
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Shuyun
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Urinary Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Fa
authors.initials: F
authors.lastname: Sun
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhou
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Wenjie
authors.initials: W
authors.lastname: Xu
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Chao
authors.initials: C
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Second Affiliated Hospital, Shantou University Medical College, Shantou, People's Republic of China.
authors.forename: Lingfei
authors.initials: L
authors.lastname: Wu
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Guidan
authors.initials: G
authors.lastname: Ye
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Zhuo
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, People's Republic of China.
authors.forename: Yongli
authors.initials: Y
authors.lastname: Huang
mesh_terms: D000328:Adult; D015415:Biomarkers / Q000378:metabolism; D001755:Blastocyst / Q000378:metabolism*; D017077:Culture Media, Conditioned / Q000378:metabolism*; D046149:Embryo Culture Techniques; D010064:Embryo Implantation; D004624:Embryo Transfer; D005260:Female; D005298:Fertility; D005307:Fertilization in Vitro / Q000009:adverse effects; D006801:Humans; D007247:Infertility, Female / Q000175:diagnosis / Q000503:physiopathology / Q000628:therapy*; D016209:Interleukin-8 / Q000378:metabolism*; D050498:Live Birth; D008875:Middle Aged; D011237:Predictive Value of Tests; D011247:Pregnancy; D018873:Pregnancy Rate; D016896:Treatment Outcome; D055815:Young Adult
chemical_list: D015415:Biomarkers; C508606:CXCL8 protein, human; D017077:Culture Media, Conditioned; D016209:Interleukin-8
keywords: Culture medium; IVF outcome; embryo quality; interleukin-8