title: Genome sequence of bubaline alphaherpesvirus 1 (BuHV1) isolated in Australia in 1972.
abstract: Bubaline alphaherpesvirus 1 (BuHV1) is a member of the family Herpesviridae, subfamily Alphaherpesvirinae, genus Varicellovirus. To date, no full genome sequence of BuHV has been published. Here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (BuHV1) strain b6 (BuHV1-b6), isolated from a water buffalo (Bubalus bubalis) in 1972 in Australia. The virus was multiplied in MDBK cells, and the DNA was extracted and subjected to high-throughput sequencing. The reads were aligned and combined into a single genome sequence, with bovine alphaherpesvirus 5 (BoHV5) strain SV507/99 (accession number NC005261) as a reference. The BuHV1-b6 genome is a linear double-stranded DNA molecule, 137,452 bp long, with a GC content of 76.8%. The genome consists of two unique sequences: a long, or UL, sequence (103,818 bp) and a short, or US, sequence (9,586 bp), with the latter being flanked by inverted IR and TR elements of 12,024 bp each. The arrangement is typical of herpesvirus genomes of the D-type. The overall sequence has a 92.2% similarity at the nucleotide level to the reference BoHV5 strain. Our report provides a significant landmark in the history of herpesviruses, represented by the genome sequence of this 44-year-old virus isolate.
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil. scheffer.cm@gmail.com.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil. scheffer.cm@gmail.com.
authors.forename: Camila Mengue
authors.initials: CM
authors.lastname: Scheffer
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Ana Paula Muterle
authors.initials: AP
authors.lastname: Varela
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Samuel Paulo
authors.initials: SP
authors.lastname: Cibulski
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Candice
authors.initials: C
authors.lastname: Schmidt
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Fabrício Souza
authors.initials: FS
authors.lastname: Campos
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Willian Pinto
authors.initials: WP
authors.lastname: Paim
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Raíssa Nunes
authors.initials: RN
authors.lastname: Dos Santos
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Thais Fumaco
authors.initials: TF
authors.lastname: Teixeira
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Márcia Regina
authors.initials: MR
authors.lastname: Loiko
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Tochetto
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Helton Fernandes
authors.initials: HF
authors.lastname: Dos Santos
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Diane Alves
authors.initials: DA
authors.lastname: de Lima
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Cristine
authors.initials: C
authors.lastname: Cerva
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.
authors.forename: Fabiana Quoos
authors.initials: FQ
authors.lastname: Mayer
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.
authors.forename: Sylio Alfredo
authors.initials: SA
authors.lastname: Petzhold
authors.affiliation: Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Ana Cláudia
authors.initials: AC
authors.lastname: Franco
authors.affiliation: Unit 44 (formerly with CSIRO Australia), 260 Cliveden Avenue, Corinda, QLD, 4075, Australia.
authors.forename: Toby St
authors.initials: TS
authors.lastname: George
authors.affiliation: Institute of Health Sciences, Feevale University, Rodovia RS-239, Novo Hamburgo, Rio Grande do Sul, CEP 93525075, Brazil.
authors.forename: Fernando Rosado
authors.initials: FR
authors.lastname: Spilki
authors.affiliation: Institute of Veterinary Research "Desidério Finamor" (IPVDF), Estrada do Conde 6000, Eldorado do Sul, Rio Grande do Sul, CEP 92990-000, Brazil.|Virology Laboratory, Department of Microbiology, Immunology and Parasitology, Institute of Basic Health Sciences, Federal University of Rio Grande do Sul (UFRGS), Rua Sarmento Leite 500, Porto Alegre, Rio Grande do Sul, CEP 90050-170, Brazil.
authors.forename: Paulo Michel
authors.initials: PM
authors.lastname: Roehe
mesh_terms: D000818:Animals; D001315:Australia; D001483:Base Sequence; D002020:Buffaloes / Q000821:virology*; D002417:Cattle; D002460:Cell Line; D004279:DNA, Viral / Q000235:genetics*; D004285:Dogs; D016679:Genome, Viral / Q000235:genetics*; D059014:High-Throughput Nucleotide Sequencing; D061985:Madin Darby Canine Kidney Cells; D017422:Sequence Analysis, DNA; D018140:Varicellovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification
chemical_list: D004279:DNA, Viral