title: The effect of Platycodin D on the expression of cytoadherence proteins P1 and P30 in Mycoplasma pneumoniae models.
abstract: Platycodin D is one of the most important monomers of the Qinbaiqingfei pellet (Qinbai), which has already been approved as the first effective new Traditional Chinese Medicine used to fight against Mycoplasma pneumoniae (M. pneumoniae) in clinic in China. In previous studies, pharmacodynamics experiment has proved that Platycodin D has anti-M. pneumoniae effect and the minimum inhibitory concentration (MIC) is 16mμg/ml. This paper further clarified that the mechanism underlying the anti-M. pneumoniae effect of Platycodin D might be due to M. pneumoniae adhesion proteins P1 and P30. P1 and P30 expression levels in M. pneumoniae strain, M. pneumoniae-infected BALB/c mice, and M. pneumoniae-infected A549 cells were determined by reverse transcription PCR. Platycodin D strongly inhibited P1 and P30 expression in M. pneumonia and high dosage of Platycodin D exhibited a greater effect on reducing P1 and P30 expression than low dose Platycodin D. Platycodin D prevented M. pneumoniae infection through inhibiting the expression of adhesion proteins, which might be one of the mechanisms for the anti-M. pneumoniae properties of Qinbai. These results provide a foundation to further explore the mechanisms of action of Qinbai in future studies.
authors.affiliation: Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China.
authors.forename: Yan-Li
authors.initials: YL
authors.lastname: Meng
authors.affiliation: Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China. Electronic address: wangweiming475@yahoo.com.
authors.forename: Wei-Ming
authors.initials: WM
authors.lastname: Wang
authors.affiliation: Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China.
authors.forename: Dan-Dan
authors.initials: DD
authors.lastname: Lv
authors.affiliation: Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China.
authors.forename: Qiu-Xia
authors.initials: QX
authors.lastname: An
authors.affiliation: Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Wei-Hong
authors.initials: WH
authors.lastname: Lu
authors.affiliation: Heilongjiang Academy of Chinese Medicine Sciences, Harbin 150036, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Guixin
authors.initials: G
authors.lastname: Tang
mesh_terms: D000072283:A549 Cells; D018829:Adhesins, Bacterial / Q000235:genetics*; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology* / Q000627:therapeutic use; D006801:Humans; D008807:Mice, Inbred BALB C; D009177:Mycoplasma pneumoniae / Q000187:drug effects* / Q000235:genetics / Q000472:pathogenicity; D011019:Pneumonia, Mycoplasma / Q000517:prevention & control; D012333:RNA, Messenger / Q000378:metabolism; D012503:Saponins / Q000494:pharmacology* / Q000627:therapeutic use; D014315:Triterpenes / Q000494:pharmacology* / Q000627:therapeutic use
chemical_list: D018829:Adhesins, Bacterial; D000900:Anti-Bacterial Agents; C096205:P30 adhesin, Mycoplasma; D012333:RNA, Messenger; D012503:Saponins; D014315:Triterpenes; C035471:adhesin, Mycoplasma pneumoniae; C108953:platycodin D
keywords: Animal models; M. pneumoniae; P1; P30; Platycodin D