title: Signal-Amplified Lateral Flow Test Strip for Visual Detection of Cu2.
abstract: A signal-amplified lateral flow test strip (SA-LFTS) for the detection of Cu2+ in aqueous solution was constructed based on Cu+-catalyzed click chemistry and hybridization of single-stranded DNA (ssDNA). Alkyne and azide modified ssDNA acted as specific elements for Cu2+ recognition, and a chemical ligation product formed through Cu+-catalyzed alkyne-azide cycloaddition. Hybridization of ssDNA-labeled gold nanoparticles resulted in high sensitivity, and the output signal could be observed directly by the naked eye. Using the developed SA-LFTS under optimal conditions, Cu2+ could be detected rapidly with limit of detections of 5 nM and 4.2 nM by visual observation and quantitative analysis, respectively. The sensitivity (i.e. the visual limit of detection) of the SA-LFTS was 80-times higher than that of traditional LFTS. The SA-LFTS was applied to the determination of Cu2+ in municipal water and river water samples with the results showing good recovery and accuracy. The developed test strip is promising for point-of-care applications and detection of Cu2+ in the field.
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, College of Plant Protection, Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Yulong
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Limin
authors.initials: L
authors.lastname: Wang
authors.affiliation: College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Juanjuan
authors.initials: J
authors.lastname: Xue
authors.affiliation: College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Jinbo
authors.initials: J
authors.lastname: Dong
authors.affiliation: College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Jia
authors.initials: J
authors.lastname: Cai
authors.affiliation: College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Xiude
authors.initials: X
authors.lastname: Hua
authors.affiliation: College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Minghua
authors.initials: M
authors.lastname: Wang
authors.affiliation: Institute of Food Quality Safety and Detection Research, Jiangsu Academy of Agricultural Sciences, Nanjing, P. R. China.
authors.forename: Cunzheng
authors.initials: C
authors.lastname: Zhang
authors.affiliation: Institute of Plant Protection, Jiangsu Academy of Agricultural Science, College of Plant Protection, Nanjing Agricultural University, Nanjing, P. R. China.|College of Plant Protection (Key Laboratory of Integrated Management of Crop Diseases and Pests), Nanjing Agricultural University, Nanjing, P. R. China.
authors.forename: Fengquan
authors.initials: F
authors.lastname: Liu
mesh_terms: D000480:Alkynes; D001386:Azides; D015374:Biosensing Techniques; D057930:Click Chemistry; D003300:Copper / Q000032:analysis*; D015342:DNA Probes; D004277:DNA, Single-Stranded; D004784:Environmental Monitoring; D006046:Gold; D053768:Metal Nanoparticles; D012680:Sensitivity and Specificity
chemical_list: D000480:Alkynes; D001386:Azides; D015342:DNA Probes; D004277:DNA, Single-Stranded; D006046:Gold; D003300:Copper