title: Sijunzi Decoction attenuates 2, 4, 6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats and ameliorates TNBS-induced claudin-2 damage via NF-κB pathway in Caco2 cells.
abstract: BACKGROUND
  SijunziDecoction (SJZD) is a traditional Chinese medicine prescription used to treat the diseases of gastrointestinal tract since ancient times. The objective of this study was to investigate the protective effects of SJZD on TNBS-induced colitis in rats and TNBS-damaged Caco2 cells.
  
  
  METHODS
  The rat colitis model was induced by 2, 4, 6-trinitrobenzene sulfonic acid (TNBS). SJZD (2.8 5.6, 11.2 g/kg) or salazosulfapyridine (SASP) (0.4 g/kg) was administrated orally in rats for 7 days. DAI, pathological scores and the expression of claudin-2 were evaluated. Then we explored the effect and mechanism of SijunziDecoction Serum (SJZDS) onTNBS-damaged Caco2 cells to figure out intestinal barrier protective effect and mechanism of SJZD.
  
  
  RESULTS
  SJZD significantly ameliorated the severity of TNBS-induced colitis and downregulated the level of claudin-2 in colonic tissues. SJZDS promoted proliferation and inhibited apoptosis ofTNBS-damaged Caco2 cells. In Caco2 cell monolayers, we provided mechanistic evidence that SJZDS-induced increased TEER and decreased permeability after TNBS damage, which were mediated through claudin-2 and NF-κB pathway, including the upregulation of claudin-2, decreased activity of NF-κB p65, reduced level of NF-κB p65 and MLCK.
  
  
  CONCLUSIONS
  Our results indicated that SJZD possesses protective effect of intestinal barrier towards TNBS-induced colitis in rats and TNBS-damaged Caco2 cells in vitro. SJZDis a potential protective agent of intestinal barrier that deserves further investigation.
authors.affiliation: The Second Clinical College, GuangzhouUniversity of Chinese Medicine, Guangzhou, 510000, China.|Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510000, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Lu
authors.affiliation: The Second Clinical College, GuangzhouUniversity of Chinese Medicine, Guangzhou, 510000, China.|Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510000, China.
authors.forename: HanJie
authors.initials: H
authors.lastname: Lin
authors.affiliation: The Second Clinical College, GuangzhouUniversity of Chinese Medicine, Guangzhou, 510000, China.|Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510000, China.
authors.forename: JinWei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: The Second Clinical College, GuangzhouUniversity of Chinese Medicine, Guangzhou, 510000, China.|Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510000, China.
authors.forename: JianAn
authors.initials: J
authors.lastname: Wei
authors.affiliation: The Second Clinical College, GuangzhouUniversity of Chinese Medicine, Guangzhou, 510000, China.|Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510000, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Sun
authors.affiliation: The Second Clinical College, GuangzhouUniversity of Chinese Medicine, Guangzhou, 510000, China. linghan36@163.com.|Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, 510000, China. linghan36@163.com.
authors.forename: Ling
authors.initials: L
authors.lastname: Han
mesh_terms: D000818:Animals; D018938:Caco-2 Cells; D062446:Claudin-2 / Q000235:genetics / Q000378:metabolism*; D003092:Colitis / Q000139:chemically induced / Q000188:drug therapy* / Q000235:genetics / Q000378:metabolism; D004365:Drugs, Chinese Herbal / Q000008:administration & dosage*; D006801:Humans; D007413:Intestinal Mucosa / Q000187:drug effects / Q000378:metabolism; D008297:Male; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism*; D051381:Rats; D015398:Signal Transduction / Q000187:drug effects; D014302:Trinitrobenzenesulfonic Acid
chemical_list: D062446:Claudin-2; D004365:Drugs, Chinese Herbal; D016328:NF-kappa B; D014302:Trinitrobenzenesulfonic Acid
keywords: Claudin-2; Inflammatory bowel disease; NF-κB pathway; SijunziDecoction; Tight junction