title: Expression and functional role of long non-coding RNA AFAP1-AS1 in ovarian cancer.
abstract: The present study aimed to determine the expression, roles and functional mechanisms of long non-coding RNA AFAP1-AS1 (AFAP1-AS1) in the progression of ovarian cancer.
  
  
  
  AFAP1-AS1 expression was assessed in ovarian cancer samples and ovarian cancer cell lines by quantitative real-time PCR. The association of AFAP1-AS1 expression with clinicopathological factors was also analyzed. Gain-/loss-of-function studies were performed to investigate the expression pattern and functional role of AFAP1-AS1 in ovarian cancer. Effects of AFAP1-AS1 on cell proliferation were studied using cell-counting kit-8 assay. The effect of AFAP1-AS1 on ovarian cancer cells apoptosis was assessed by flow cytometry assay.
  
  
  
  AFAP1-AS1 was highly expressed both in ovarian cancer samples and cell lines compared with their corresponding normal counterparts. High AFAP1-AS1 expression was significantly associated with response and FIGO stage. Gain-of-function and loss-of-function experiments showed that AFAP1-AS1 promoted ovarian cancer cells proliferation. Flow cytometry assay showed that knockdown of AFAP1-AS1 promoted ovarian cancer cells apoptosis.
  
  
  
  Our results suggested that AFAP1-AS1 might be considered as a novel oncogene involved in ovarian cancer progression.
authors.affiliation: Department of Obstetrics and Gynecology, The Second Hospital of Jilin University, Changchun, China. ff76ff1@163.com.
authors.forename: S-L
authors.initials: SL
authors.lastname: Yang
authors.forename: R-X
authors.initials: RX
authors.lastname: Lin
authors.forename: L-H
authors.initials: LH
authors.lastname: Si
authors.forename: M-H
authors.initials: MH
authors.lastname: Cui
authors.forename: X-W
authors.initials: XW
authors.lastname: Zhang
authors.forename: L-M
authors.initials: LM
authors.lastname: Fan
mesh_terms: D017209:Apoptosis; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D005260:Female; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D009857:Oncogenes; D010051:Ovarian Neoplasms / Q000235:genetics*; D062085:RNA, Long Noncoding / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction
chemical_list: C000605367:AFAP1-AS1 long noncoding RNA, human; D062085:RNA, Long Noncoding