title: Paramagnetic Manganese in the Atherosclerotic Plaque of Carotid Arteries.
abstract: The search for adequate markers of atherosclerotic plaque (AP) instability in the context of assessment of the ischemic stroke risk in patients with atherosclerosis of the carotid arteries as well as for solid physical and chemical factors that are connected with the AP stability is extremely important. We investigate the inner lining of the carotid artery specimens from the male patients with atherosclerosis (27 patients, 42-64 years old) obtained during carotid endarterectomy by using different analytical tools including ultrasound angiography, X-ray analysis, immunological, histochemical analyses, and high-field (3.4 T) pulse electron paramagnetic resonance (EPR) at 94 GHz. No correlation between the stable and unstable APs in the sense of the calcification is revealed. In all of the investigated samples, the EPR spectra of manganese, namely, Mn2+ions, are registered. Spectral and relaxation characteristics of Mn2+ions are close to those obtained for the synthetic (nano) hydroxyapatite species but differ from each other for stable and unstable APs. This demonstrates that AP stability could be specified by the molecular organization of their hydroxyapatite components. The origin of the obtained differences and the possibility of using EPR of Mn2+as an AP stability marker are discussed.
authors.affiliation: Kazan Federal University, Kazan, Russia; Kazan Medical University, Kazan, Russia.
authors.forename: Yury
authors.initials: Y
authors.lastname: Chelyshev
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Marat
authors.identifier: 0000-0002-2179-2823
authors.initials: M
authors.lastname: Gafurov
authors.affiliation: Interregional Clinical Diagnostic Centre (ICDC), Kazan, Russia.
authors.forename: Igor
authors.initials: I
authors.lastname: Ignatyev
authors.affiliation: Interregional Clinical Diagnostic Centre (ICDC), Kazan, Russia.
authors.forename: Alexey
authors.initials: A
authors.lastname: Zanochkin
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Georgy
authors.initials: G
authors.lastname: Mamin
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Boris
authors.initials: B
authors.lastname: Sorokin
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Alexandra
authors.initials: A
authors.lastname: Sorokina
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Natalya
authors.initials: N
authors.lastname: Lyapkalo
authors.affiliation: Interregional Clinical Diagnostic Centre (ICDC), Kazan, Russia.
authors.forename: Nazima
authors.initials: N
authors.lastname: Gizatullina
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Yana
authors.identifier: 0000-0002-9435-340X
authors.initials: Y
authors.lastname: Mukhamedshina
authors.affiliation: Kazan Federal University, Kazan, Russia.
authors.forename: Sergei
authors.initials: S
authors.lastname: Orlinskii
mesh_terms: D000328:Adult; D050197:Atherosclerosis / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery; D002339:Carotid Arteries / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000601:surgery; D017886:Durapatite / Q000378:metabolism; D004578:Electron Spin Resonance Spectroscopy; D016894:Endarterectomy, Carotid; D006801:Humans; D008297:Male; D008345:Manganese / Q000378:metabolism*; D008875:Middle Aged; D058226:Plaque, Atherosclerotic / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000503:physiopathology / Q000601:surgery
chemical_list: D008345:Manganese; D017886:Durapatite