title: Inhibitory effect of miR-145 on RPE cell proliferation.
abstract: OBJECTIVE
  This study aims to explore the impact of micro RNA miR-145 on retinal pigment epithelial cell proliferation and apoptosis.
  
  
  METHODS
  A stable culture and passage system of hPNE cells was first established, and its migration ability was determined. Then, miR-145 lentiviral vectors were constructed to transfect hPRE cells. Thereafter, hRPE cell proliferation was detected by MTT assay after they were transfected by lentivirus, cell cycle was analyzed by flow cytometry, and apoptosis was detected by Annexin V/PI double staining immunofluorescence.
  
  
  RESULTS
  Cultured hPRE cells had good migrating and metastatic ability, in which subsequent lentivirus infection experiments can be carried out. After transfection by miR-145 lentiviral vectors, hPRE cell proliferation slowed down and RPE cells in the G1phase was inhibited; thus, apoptosis rate increased.
  
  
  CONCLUSIONS
  MiR-145 can slow down retinal pigment epithelial cell proliferation and increase their apoptosis rate. This has a certain therapeutic potential for diseases caused by RPE cell proliferation such as PVR.
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Hubei Polytechnic College; The People's Hospital of Daye City Daye 435100, Hubei Province, P. R. China.
authors.forename: Ken
authors.initials: K
authors.lastname: Zhao
authors.affiliation: Department of Ophthalmology, Renmin Hospital of Wuhan University Wuhan 430060, Hubei Province, P. R. China.
authors.forename: Zhen
authors.initials: Z
authors.lastname: Chen
authors.affiliation: Department of Ophthalmology, Xianning Central Hospital, The Fist Affiliated Hospital Of Hubei University Of Science And Technology Xianning 437100, Hubei Province, P. R. China.
authors.forename: Xu-Dong
authors.initials: XD
authors.lastname: Lv
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Hubei Polytechnic College; The People's Hospital of Daye City Daye 435100, Hubei Province, P. R. China.
authors.forename: Gang
authors.initials: G
authors.lastname: Dong
authors.affiliation: Department of Ophthalmology, The Second Affiliated Hospital of Hubei Polytechnic College; The People's Hospital of Daye City Daye 435100, Hubei Province, P. R. China.
authors.forename: Huan
authors.initials: H
authors.lastname: Xia
keywords: RPE cells; apoptosis; lentiviruses; miR-145; proliferation