title: Short communication: Progressive motility of frozen-thawed canine semen is highest five minutes after thawing.
abstract: Progressive motility is usually estimated by visual inspection using a light contrast microscope at X 100 immediately after semen collection or immediately after thawing frozen semen. Standard operating procedures have never been established for this test. The objective of this experiment was to examine time-dependent changes of motility after thawing cryopreserved canine semen. Semen of 35 dogs was collected, and volume, concentration, progressive motility, morphology, membrane integrity and HOS test were evaluated. For cryopreservation, CaniPRO®Freeze A&B was used. Semen was thawed and diluted using CaniPRO®culture medium. After thawing, semen was evaluated as before. In addition, every sample was evaluated for progressively motile sperm cells 0, 5, 20 and 60 min after thawing. Progressive semen motility was significantly highest five minutes after thawing.
authors.affiliation: Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität, Berlin, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Karger
authors.affiliation: Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität, Berlin, Germany.
authors.forename: B
authors.initials: B
authors.lastname: Geiser
authors.affiliation: Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität, Berlin, Germany.
authors.forename: M
authors.initials: M
authors.lastname: Grau
authors.affiliation: Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität, Berlin, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Heuwieser
authors.affiliation: Clinic for Animal Reproduction, Faculty of Veterinary Medicine, Freie Universität, Berlin, Germany.
authors.forename: S P
authors.initials: SP
authors.lastname: Arlt
mesh_terms: D000818:Animals; D015925:Cryopreservation / Q000662:veterinary*; D004285:Dogs; D008297:Male; D012662:Semen Preservation / Q000379:methods / Q000662:veterinary*; D013081:Sperm Motility / Q000502:physiology*; D013094:Spermatozoa / Q000502:physiology*; D013997:Time Factors
keywords: cryopreservation; dogs; freezing; motility; semen; thawing