title: The effect of serum and follicular fluid anti-Mullerian hormone level on the number of oocytes retrieved and rate of fertilization and clinical pregnancy.
abstract: OBJECTIVE
  The objective of this study was to evaluate the relationship between oocyte yield, fertilization, and clinical pregnancy (CP), and anti-Mullerian hormone (AMH) level in serum and follicular fluid during in vitro fertilization treatment.
  
  
  METHODS
  Forty-four infertile women who underwent IVF treatment using multiagonist protocol were included in this study. Baseline level of AMH in serum and follicular fluid was measured on third day of menstrual cycle. AMH level in serum and follicular fluid was then measured again on day of oocyte pick-up. Pearson correlation and binary regression tests were used for statistical analysis. For Type 1 error, p=5% was selected as cut-off value for statistical significance.
  
  
  RESULTS
  Serum AMH level was positively correlated with total number of oocytes retrieved and rate of fertilization and CP (r=0.397, p=0.008; r=0.401, p=0.007; and r=0.382, p=0.011, respectively). There was significantly negative correlation between serum level of follicle-stimulating hormone (FSH) and fertilization rate (r=-0.320; p=0.034), as well as serum FSH level and CP rate (r=-0.308; p=0.042). There were no significant correlations between AMH level in follicular fluid and IVF treatment outcomes.
  
  
  CONCLUSIONS
  Serum AMH levels may be more reliable for prediction of total number of oocytes retrieved and rate of fertilization and CP than AMH levels in follicular fluid.
authors.affiliation: Department of Obstetrics and Gynecology, Yalvac State Hospital, Yalvac, Isparta, Turkey.
authors.forename: Seda Eymen
authors.initials: SE
authors.lastname: Bolat
authors.affiliation: Department of Gynecology, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: Safak
authors.initials: S
authors.lastname: Ozdemirci
authors.affiliation: Department of Gynecology Risky Pregnancy, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: Taner
authors.initials: T
authors.lastname: Kasapoglu
authors.affiliation: Department of Obstetrics and Gynecology, Abant Izzet Baysal University Faculty of Medicine, Bolu, Turkey.
authors.forename: Bulent
authors.initials: B
authors.lastname: Duran
authors.affiliation: Vocational School of Health Services, Igdir University, Igdir, Turkey.
authors.forename: Levent
authors.initials: L
authors.lastname: Goktas
authors.affiliation: Department of Gynecology Risky Pregnancy, Etlik Zubeyde Hanim Women's Health Training and Research Hospital, Ankara, Turkey.
authors.forename: Ertugrul
authors.initials: E
authors.lastname: Karahanoglu
keywords: Anti-Mullerian hormone (AMH); clinical pregnancy; fertilization; follicle-stimulating hormone (FSH); follicular fluid; in vitro fertilization; infertility