title: Influence of Mechanical Force on Bone Matrix Proteins in Ovariectomised Mice and Osteoblast-like MC3T3-E1 Cells.
abstract: To investigate the effect of mechanical stress on periostin and semaphorin-3A expression in a murine model of postmenopausal osteoporosis and in osteoblast-like MC3T3-E1 cells.
  
  
  
  Female mice were divided into three groups and treated with a sham operation, ovariectomy (OVX) or OVX plus treadmill training (OVX+Run). After 10 weeks, tibias were used for histological analysis. MC3T3-E1 cells were burdened by mechanical stress using a centrifuge or were treated with periostin, and the production of biologically-active semaphorin-3A was examined in vitro.
  
  
  
  In OVX+Run group tibias, the number of tartrate-resistant acid phosphatase-positive osteoclasts was lower than in the OVX group, and the expression of periostin and semaphorin-3A was higher. In MC3T3-E1 cells, centrifugal stress significantly increased periostin and semaphorin-3A mRNA expression. Treatment with periostin increased the semaphorin-3A level.
  
  
  
  We speculate that mechanical load may increase periostin production in osteoblasts, and periostin may inhibit osteoclast differentiation by its effects on semaphorin-3A. Our results support the concept of a positive correlation between exercise and inhibition of osteoclasts in post-menopausal osteoporosis.
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
authors.forename: Meng
authors.initials: M
authors.lastname: Zhang
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan s-ishikawa@med.showa-u.ac.jp.
authors.forename: Shintaro
authors.initials: S
authors.lastname: Ishikawa
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
authors.forename: Tomoko
authors.initials: T
authors.lastname: Inagawa
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
authors.forename: Hideshi
authors.initials: H
authors.lastname: Ikemoto
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
authors.forename: Shiyu
authors.initials: S
authors.lastname: Guo
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
authors.forename: Masataka
authors.initials: M
authors.lastname: Sunagawa
authors.affiliation: Department of Physiology, School of Medicine, Showa University, Tokyo, Japan.
authors.forename: Tadashi
authors.initials: T
authors.lastname: Hisamitsu
mesh_terms: D000818:Animals; D015815:Cell Adhesion Molecules / Q000494:pharmacology*; D004195:Disease Models, Animal; D004797:Enzyme-Linked Immunosorbent Assay; D016326:Extracellular Matrix Proteins / Q000235:genetics / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation / Q000187:drug effects*; D006801:Humans; D007124:Immunoenzyme Techniques; D051379:Mice; D008813:Mice, Inbred ICR; D010006:Osteoblasts / Q000166:cytology / Q000187:drug effects / Q000378:metabolism*; D015663:Osteoporosis, Postmenopausal / Q000188:drug therapy / Q000209:etiology / Q000378:metabolism*; D010052:Ovariectomy; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D040121:Semaphorin-3A / Q000235:genetics / Q000378:metabolism*; D013314:Stress, Mechanical
chemical_list: D015815:Cell Adhesion Molecules; D016326:Extracellular Matrix Proteins; C512835:POSTN protein, human; D040121:Semaphorin-3A
keywords: Osteoclast; osteoblast; osteoporosis; periostin; semaphorin-3A