title: Short Communication: Immunohistochemical localization of the immune cell marker CD68 in bovine adipose tissue: impact of tissue alterations and excessive fat accumulation in dairy cows.
abstract: With the onset of lactation energy from feed intake is mostly insufficient to meet the requirements of dairy cows. Lipid mobilization from adipose tissue (AT) could lead to a compromised inflammatory response enhancing the incidence for diseases. In addition, tissue alterations can occur, displaying areas of necrosis and inflammation. Furthermore, over-conditioned cows mobilizing more lipids from AT than thin cows are prone to develop metabolic disorders. This might lead to an increased infiltration of phagocytic immune cells into AT. In the present study, CD68 positive cells were localized in AT from 10 early lactating Holstein cows displaying different grades of AT alterations. Biopsies were sampled from visceral and subcutaneous AT and the number of CD68 positive cells was immunohistochemically determined. In addition, AT biopsies from over-conditioned, non-pregnant, non-lactating cows (n=8) were immunohistochemically analyzed for CD68 positive cells. The percentage of CD68 positive cells was less than 2% in AT biopsies with tissue alterations and in AT from over-conditioned cows. Therefore, immune cell infiltration demonstrated via the localization of CD68 positive cells seems to play only a minor role in AT from over-conditioned cows as well as in different bovine AT depots with tissue alterations.
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, 53115 Bonn, Germany. Electronic address: susanne.haeussler@uni-bonn.de.
authors.forename: S
authors.initials: S
authors.lastname: Häussler
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: D
authors.initials: D
authors.lastname: Germeroth
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: L
authors.initials: L
authors.lastname: Laubenthal
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
authors.forename: L F
authors.initials: LF
authors.lastname: Ruda
authors.affiliation: Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, 30173 Hannover, Germany.
authors.forename: J
authors.initials: J
authors.lastname: Rehage
authors.affiliation: Institute of Animal Nutrition, Friedrich-Loeffler-Institute (FLI), Federal Research Institute for Animal Health, 38116 Braunschweig, Germany.
authors.forename: S
authors.initials: S
authors.lastname: Dänicke
authors.affiliation: Institute of Animal Science, Physiology and Hygiene Unit, University of Bonn, 53115 Bonn, Germany.
authors.forename: H
authors.initials: H
authors.lastname: Sauerwein
mesh_terms: D000273:Adipose Tissue / Q000166:cytology / Q000276:immunology* / Q000473:pathology; D000818:Animals; D015703:Antigens, CD / Q000032:analysis* / Q000276:immunology; D015214:Antigens, Differentiation, Myelomonocytic / Q000032:analysis* / Q000276:immunology; D001706:Biopsy / Q000662:veterinary; D002417:Cattle / Q000033:anatomy & histology / Q000276:immunology*; D004032:Diet; D005260:Female; D007150:Immunohistochemistry / Q000662:veterinary; D008264:Macrophages / Q000276:immunology; D000097382:CD68 Molecule
chemical_list: D015703:Antigens, CD; D015214:Antigens, Differentiation, Myelomonocytic; C067980:CD68 antigen, human; D000097382:CD68 Molecule
keywords: Adipose tissue; CD68; Dairy cow