title: Diversity in VP3, NSP3, and NSP4 of rotavirus B detected from Japanese cattle.
abstract: Bovine rotavirus B (RVB) is an etiological agent of diarrhea mostly in adult cattle. Currently, a few sequences of viral protein (VP)1, 2, 4, 6, and 7 and nonstructural protein (NSP)1, 2, and 5 of bovine RVB are available in the DDBJ/EMBL/GenBank databases, and none have been reported for VP3, NSP3, and NSP4. In order to fill this gap in the genetic characterization of bovine RVB strains, we used a metagenomics approach and sequenced and analyzed the complete coding sequences (CDS) of VP3, NSP3, and NSP4 genes, as well as the partial or complete CDS of other genes of RVBs detected from Japanese cattle. VP3, NSP3, and NSP4 of bovine RVBs shared low nucleotide sequence identities (63.3-64.9% for VP3, 65.9-68.2% for NSP3, and 52.6-56.2% for NSP4) with those of murine, human, and porcine RVBs, suggesting that bovine RVBs belong to a novel genotype. Furthermore, significantly low amino acid sequence identities were observed for NSP4 (36.1-39.3%) between bovine RVBs and the RVBs of other species. In contrast, hydrophobic plot analysis of NSP4 revealed profiles similar to those of RVBs of other species and rotavirus A (RVA) strains. Phylogenetic analyses of all gene segments revealed that bovine RVB strains formed a cluster that branched distantly from other RVBs. These results suggest that bovine RVBs have evolved independently from other RVBs but in a similar manner to other rotaviruses. These findings provide insights into the evolution and diversity of RVB strains.
authors.affiliation: Ishikawa Prefectural Livestock Research Center, Hodatsushimizu, Ishikawa 929-1325, Japan.
authors.forename: Michiko
authors.initials: M
authors.lastname: Hayashi-Miyamoto
authors.affiliation: Ishikawa Hokubu Livestock Hygiene Service Center, Nanao, Ishikawa 929-2126, Japan.
authors.forename: Toshiaki
authors.initials: T
authors.lastname: Murakami
authors.affiliation: Ishikawa Hokubu Livestock Hygiene Service Center, Nanao, Ishikawa 929-2126, Japan.
authors.forename: Fujiko
authors.initials: F
authors.lastname: Minami-Fukuda
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Shinobu
authors.initials: S
authors.lastname: Tsuchiaka
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Mai
authors.initials: M
authors.lastname: Kishimoto
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Kaori
authors.initials: K
authors.lastname: Sano
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Yuki
authors.initials: Y
authors.lastname: Naoi
authors.affiliation: Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan.
authors.forename: Keigo
authors.initials: K
authors.lastname: Asano
authors.affiliation: Department of Health and Medical Sciences, Ishikawa Prefectural Nursing University, Kahoku, Ishikawa 929-1210, Japan.
authors.forename: Toru
authors.initials: T
authors.lastname: Ichimaru
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan; Laboratory of Viral Infection I, Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Minato, Tokyo 108-8641, Japan.
authors.forename: Kei
authors.initials: K
authors.lastname: Haga
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Tsutomu
authors.initials: T
authors.lastname: Omatsu
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Yukie
authors.initials: Y
authors.lastname: Katayama
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Mami
authors.initials: M
authors.lastname: Oba
authors.affiliation: Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Musashino, Tokyo 180-8602, Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Aoki
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan; Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Junsuke
authors.initials: J
authors.lastname: Shirai
authors.affiliation: Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan.
authors.forename: Motohiko
authors.initials: M
authors.lastname: Ishida
authors.affiliation: Department of Virology II, National Institute of Infectious Diseases, Musashimurayama, Tokyo 208-0011, Japan; Laboratory of Viral Infection I, Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Minato, Tokyo 108-8641, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Katayama
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Tetsuya
authors.initials: T
authors.lastname: Mizutani
authors.affiliation: Research and Education Center for Prevention of Global Infectious Disease of Animal, Tokyo University of Agriculture and Technology, Fuchu, Tokyo 183-8509, Japan; Department of Bioproduction Science, Ishikawa Prefectural University, Nonoichi, Ishikawa 921-8836, Japan. Electronic address: m-nagai@ishikawa-pu.ac.jp.
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
mesh_terms: D000818:Animals; D005075:Biological Evolution; D036022:Capsid Proteins / Q000235:genetics*; D002417:Cattle; D016000:Cluster Analysis; D030541:Databases, Genetic; D005243:Feces / Q000821:virology; D014644:Genetic Variation; D016679:Genome, Viral; D005838:Genotype; D006023:Glycoproteins / Q000235:genetics*; D007564:Japan / Q000453:epidemiology; D010802:Phylogeny; D012401:Rotavirus / Q000145:classification / Q000235:genetics*; D012400:Rotavirus Infections / Q000453:epidemiology / Q000821:virology; D014118:Toxins, Biological / Q000235:genetics*; D017361:Viral Nonstructural Proteins / Q000235:genetics*
chemical_list: D036022:Capsid Proteins; D006023:Glycoproteins; C081369:NS28 protein, rotavirus; C082740:NSP3 protein, Rotavirus; D014118:Toxins, Biological; C442546:VP3 protein, Rotavirus; D017361:Viral Nonstructural Proteins
keywords: Bovine rotavirus B; Genetic characterization; Japan; Metagenomics approach; Novel genotype