title: Alendronate-anchored PEGylation of ceria nanoparticles promotes human hepatoma cell proliferation via AKT/ERK signaling pathways.
abstract: Previous work has suggested that ceria nanoparticles (CNPs) have regenerative antioxidant properties, which have motivated researchers to consider CNPs as therapeutic agents for treating a number of diseases, including cancer. Recent studies have shown CNPs to be toxic to cancer cells, to inhibit invasion and sensitize cancer cells to radiotherapy. In addition, several hydrophilic polymers have been used to coat the CNP surface in order to enhance its properties of extensive biocompatibility and systemic nontoxicity to normal cells and tissues. However, the results of previous studies were based on high CNP doses (10 μg/mL or more), and these doses may cause serious side effects in clinical applications. The impact of low CNP doses on tumor cells remains unknown. In this study, we report experiments indicating that CNPs-AL- polyethylene glycol (PEG)600, a type of surface-modified CNP that is more stable and less toxic than traditional CNPs could promote proliferation of hepatoma cells in a dose-dependent manner. In addition, further research showed that a low dose (0.01 μg/mL) of CNPs-AL-PEG600 could reduce hepatoma cell apoptosis and activate AKT/ERK signaling pathways. These results may provide information that is important for using CNPs-AL-PEG600 as a therapeutic agent in clinical cancer treatments.
authors.affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Heng
authors.initials: H
authors.lastname: Cheng
authors.affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Zhong-Li
authors.initials: ZL
authors.lastname: Liao
authors.affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Lin-Hong
authors.initials: LH
authors.lastname: Ning
authors.affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Hong-Yan
authors.initials: HY
authors.lastname: Chen
authors.affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Shan-Shan
authors.initials: SS
authors.lastname: Wei
authors.affiliation: Department of Biomedical Materials Science, School of Biomedical Engineering, Third Military Medical University, Chongqing, 400038, China.
authors.forename: Xiao-Chao
authors.initials: XC
authors.lastname: Yang
authors.affiliation: Department of Gastroenterology, Xinqiao Hospital, Third Military Medical University, Chongqing, 400037, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Guo
mesh_terms: D019386:Alendronate / Q000008:administration & dosage* / Q000494:pharmacology; D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D006528:Carcinoma, Hepatocellular / Q000235:genetics*; D045744:Cell Line, Tumor; D049109:Cell Proliferation; D002563:Cerium / Q000737:chemistry*; D004305:Dose-Response Relationship, Drug; D020869:Gene Expression Profiling; D056945:Hep G2 Cells; D006801:Humans; D008113:Liver Neoplasms / Q000378:metabolism*; D020935:MAP Kinase Signaling System / Q000187:drug effects*; D051379:Mice; D008819:Mice, Nude; D053758:Nanoparticles / Q000737:chemistry; D009368:Neoplasm Transplantation; D020411:Oligonucleotide Array Sequence Analysis; D011092:Polyethylene Glycols / Q000737:chemistry; D051057:Proto-Oncogene Proteins c-akt / Q000235:genetics*
chemical_list: D002563:Cerium; D011092:Polyethylene Glycols; D051057:Proto-Oncogene Proteins c-akt; D019386:Alendronate
keywords: AKT/ERK signaling pathways; alendronate-anchored; cerium oxide nanoparticles (CNPs); hepatoma; proliferation