title: Characterization of Group 2 Innate Lymphoid Cells in Allergic Airway Inflammation Models in the Mouse.
abstract: Allergic asthma is a chronic inflammatory lung disease mediated by type 2 cytokines produced by T helper 2 (Th2) cells as well as the recently discovered group 2 innate lymphoid cells (ILC2). Due to a lack of unique markers, the accurate phenotypic characterization and quantification of ILC2 requires a comprehensive panel of fluorescently labeled antibodies. The markers that are currently used to characterize ILC2 have not been standardized and often vary between research groups, which poses significant challenges when comparing data. Intranasal administration of the pro-inflammatory cytokine IL-33 in mice is associated with strong, Th2 cell-independent ILC2 activation. ILC2 are also activated in mouse models of allergic asthma based on the physiologically relevant house dust mite (HDM) allergen, which parallel eosinophilic airway inflammation observed in asthma patients. Here, we describe the analysis of ILC2 by flow cytometry in these two commonly used allergic airway inflammation models in the mouse.
authors.affiliation: Department of Pulmonary Medicine, Erasmus MC Rotterdam, 2040, 3000, CA, Rotterdam, The Netherlands.
authors.forename: Bobby W S
authors.initials: BWS
authors.lastname: Li
authors.affiliation: Department of Pulmonary Medicine, Erasmus MC Rotterdam, 2040, 3000, CA, Rotterdam, The Netherlands.
authors.forename: Dior M J M
authors.initials: DMJM
authors.lastname: Beerens
authors.affiliation: Department of Pulmonary Medicine, Erasmus MC Rotterdam, 2040, 3000, CA, Rotterdam, The Netherlands.
authors.forename: Maarten D
authors.initials: MD
authors.lastname: Brem
authors.affiliation: Department of Pulmonary Medicine, Erasmus MC Rotterdam, 2040, 3000, CA, Rotterdam, The Netherlands. r.hendriks@erasmusmc.nl.
authors.forename: Rudi W
authors.initials: RW
authors.lastname: Hendriks
mesh_terms: D000281:Administration, Intranasal; D000485:Allergens / Q000008:administration & dosage*; D000818:Animals; D000906:Antibodies / Q000737:chemistry; D001249:Asthma / Q000139:chemically induced / Q000276:immunology* / Q000473:pathology; D015415:Biomarkers / Q000032:analysis; D001992:Bronchoalveolar Lavage Fluid / Q000166:cytology / Q000276:immunology; D019070:Cell Lineage / Q000276:immunology; D004195:Disease Models, Animal; D005260:Female; D005434:Flow Cytometry / Q000295:instrumentation; D006801:Humans; D006967:Hypersensitivity / Q000276:immunology* / Q000473:pathology; D016130:Immunophenotyping / Q000379:methods*; D000067596:Interleukin-33 / Q000008:administration & dosage*; D008168:Lung / Q000276:immunology / Q000473:pathology; D008213:Lymphocyte Activation; D008214:Lymphocytes / Q000145:classification / Q000276:immunology* / Q000473:pathology; D051379:Mice; D008810:Mice, Inbred C57BL; D039981:Pyroglyphidae / Q000737:chemistry / Q000276:immunology; D013194:Staining and Labeling / Q000379:methods
chemical_list: D000485:Allergens; D000906:Antibodies; D015415:Biomarkers; C506535:Il33 protein, mouse; D000067596:Interleukin-33
keywords: Allergy and asthma; Group 2 innate lymphoid cells (ILC2); House dust mite; Interleukin-33; Murine airway inflammation model