title: Lipid Emulsions Containing Medium Chain Triacylglycerols Blunt Bradykinin-Induced Endothelium-Dependent Relaxation in Porcine Coronary Artery Rings.
abstract: Lipid emulsions for parenteral nutrition are used to provide calories and essential fatty acids for patients. They have been associated with hypertriglyceridemia, hypercholesterolemia, and metabolic stress, which may promote the development of endothelial dysfunction in patients. The aim of the present study was to determine whether five different industrial lipid emulsions may affect the endothelial function of coronary arteries. Porcine coronary artery rings were incubated with lipid emulsions 0.5, 1, or 2% (v/v) for 30 min before the determination of vascular reactivity in organ chambers and the level of oxidative stress using electron paramagnetic resonance. Incubation of coronary artery rings with either Lipidem®, Medialipid®containing long- and medium-chain triacylglycerols (LCT/MCT), or SMOFlipid®containing LCT, MCT, omega-9, and -3, significantly reduced the bradykinin-induced endothelium-dependent relaxation, affecting both the nitric oxide (NO) and endothelium-dependent hyperpolarization (EDH) components, whereas, Intralipid®containing LCT (soybean oil) and ClinOleic®containing LCT (soybean and olive oil) did not have such an effect. The endothelial dysfunction induced by Lipidem®was significantly improved by indomethacin, a cyclooxygenase (COX) inhibitor, inhibitors of oxidative stress (N-acetylcysteine, superoxide dismutase, catalase) and transition metal chelating agents (neocuproine, tetrathiomolybdate, deferoxamine and L-histidine). Lipidem®significantly increased the arterial level of oxidative stress. The present findings indicate that lipid emulsions containing LCT/MCT induce endothelial dysfunction in coronary artery rings by blunting both NO- and EDH-mediated relaxations. The Lipidem®-induced endothelial dysfunction is associated with increased vascular oxidative stress and the formation of COX-derived vasoconstrictor prostanoids.
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France.
authors.forename: Said
authors.initials: S
authors.lastname: Amissi
authors.affiliation: EA 7293, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Faculté de Médecine, Université de Strasbourg, Strasbourg, France.|Service de Réanimation Médicale, Nouvel Hôpital Civil, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
authors.forename: Julie
authors.initials: J
authors.lastname: Boisramé-Helms
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France.
authors.forename: Mélanie
authors.initials: M
authors.lastname: Burban
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France.
authors.forename: Sherzad K
authors.initials: SK
authors.lastname: Rashid
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France.
authors.forename: Antonio J
authors.initials: AJ
authors.lastname: León-González
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France.
authors.forename: Cyril
authors.initials: C
authors.lastname: Auger
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Toti
authors.affiliation: EA 7293, Fédération de Médecine Translationnelle de Strasbourg (FMTS), Faculté de Médecine, Université de Strasbourg, Strasbourg, France.|Service de Réanimation Médicale, Nouvel Hôpital Civil, Hôpitaux Universitaires de Strasbourg, Strasbourg, France.
authors.forename: Ferhat
authors.initials: F
authors.lastname: Meziani
authors.affiliation: UMR CNRS 7213, Laboratoire de Biophotonique et Pharmacologie, Faculté de Pharmacie, Université de Strasbourg, 74, route du Rhin, 67401, Illkirch, France. valerie.schini-kerth@unistra.fr.
authors.forename: Valérie B
authors.initials: VB
authors.lastname: Schini-Kerth
mesh_terms: D000818:Animals; D001920:Bradykinin / Q000378:metabolism*; D003331:Coronary Vessels / Q000187:drug effects*; D004195:Disease Models, Animal; D004578:Electron Spin Resonance Spectroscopy; D004655:Emulsions / Q000494:pharmacology; D004727:Endothelium / Q000187:drug effects*; D006801:Humans; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects; D013024:Soybean Oil / Q000494:pharmacology; D013552:Swine; D014280:Triglycerides / Q000494:pharmacology*
chemical_list: D004655:Emulsions; D014280:Triglycerides; D013024:Soybean Oil; D001920:Bradykinin
keywords: Cyclooxygenase; Endothelial dysfunction; Lipid emulsion; Medium-chain triacylglycerols; Oxidative stress