title: Covalently Connected Polymer-Protein Nanostructures Fabricated by a Reactive Self-Assembly Approach.
abstract: The synthesis of polymer-protein nanostructures opens up a new avenue for the development of new biomaterials. In this research, covalently connected polymer-protein nanostructures were fabricated through a reactive self-assembly approach. Poly(tert-butyl methacrylate-co-pyridyl disulfide methacrylamide) (PtBMA-co-PPDSMA) was synthesized by reversible addition fragmentation chain transfer (RAFT) polymerization. Covalently connected nanostructures (CCNs) with hydrophobic polymer cores and hydrophilic protein coronae were prepared by adding solutions of PtBMA-co-PPDSMA/DMF to aqueous solutions of bovine serum albumin (BSA). The thiol-disulfide exchange reaction between pyridyl disulfide groups on the polymer chains and thiol groups on the protein molecules plays a key role in the fabrication of CCNs. The self-assembly process was investigated by dynamic light scattering (DLS) and stopped-flow techniques. DLS results indicated that the sizes of the CCNs were determined by the initial polymer concentration, the BSA concentration, and the average number of thiol groups on BSA molecules. TEM and sodium dodecyl sulfate polyacrylamide gel electrophoresis were used to analyze the nanostructures. Far-UV circular dichroism results demonstrated that the original folded conformations of BSA molecules were basically maintained in the reactive self-assembly process. Compared with native BSA, the secondary structure and conformation change of coronal BSA induced by urea or thermal treatment were remarkably suppressed. The cytotoxicity assays demonstrated that the CCNs were essentially nontoxic to Hela and COS-7 cells.
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Collaborative Innovation Center of Chemical, Science and Engineering (Tianjin), Nankai University, P.R. China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Ju
authors.affiliation: Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, P.R. China.
authors.forename: Cheng
authors.initials: C
authors.lastname: Xing
authors.affiliation: The Institute of Seawater Desalination and Multipurpose Utilization, SOA, Tianjin, 300192, P.R. China.
authors.forename: Dongxia
authors.initials: D
authors.lastname: Wu
authors.affiliation: The Institute of Seawater Desalination and Multipurpose Utilization, SOA, Tianjin, 300192, P.R. China.
authors.forename: Yunfang
authors.initials: Y
authors.lastname: Wu
authors.affiliation: Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Nankai University, P.R. China.
authors.forename: Lianyong
authors.initials: L
authors.lastname: Wang
authors.affiliation: Key Laboratory of Functional Polymer Materials, Ministry of Education, College of Chemistry, Collaborative Innovation Center of Chemical, Science and Engineering (Tianjin), Nankai University, P.R. China.
authors.forename: Hanying
authors.identifier: http://orcid.org/0000-0002-0706-9188
authors.initials: H
authors.lastname: Zhao
mesh_terms: D000818:Animals; D019556:COS Cells; D018929:Cell Culture Techniques; D002470:Cell Survival; D002522:Chlorocebus aethiops; D006367:HeLa Cells; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D008689:Methacrylates / Q000737:chemistry*; D049329:Nanostructures / Q000737:chemistry* / Q000633:toxicity; D010316:Particle Size; D058105:Polymerization; D011487:Protein Conformation; D055503:Protein Multimerization; D012710:Serum Albumin, Bovine / Q000737:chemistry*; D013499:Surface Properties
chemical_list: D008689:Methacrylates; D012710:Serum Albumin, Bovine
keywords: nanostructures; polymers; proteins; self-assembly; synthesis design