title: Preparation of Cell Cultures and Vaccinia Virus Stocks.
abstract: The culturing of cell lines used with vaccinia virus, both as monolayer and in suspension, is described. The preparation of chick embryo fibroblasts (CEF) is presented for use in the production of the highly attenuated and host range-restricted modified vaccinia virus Ankara (MVA) strain of vaccinia virus. Protocols for the preparation, titration, and trypsinization of vaccinia virus stocks, as well as viral DNA preparation and virus purification methods are also included. © 2017 by John Wiley & Sons, Inc.
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Catherine A
authors.initials: CA
authors.lastname: Cotter
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Patricia L
authors.initials: PL
authors.lastname: Earl
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Linda S
authors.initials: LS
authors.lastname: Wyatt
authors.affiliation: Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland.
authors.forename: Bernard
authors.initials: B
authors.lastname: Moss
mesh_terms: D000818:Animals; D018929:Cell Culture Techniques / Q000379:methods*; D002460:Cell Line; D002478:Cells, Cultured; D002642:Chick Embryo; D004279:DNA, Viral / Q000302:isolation & purification; D005347:Fibroblasts / Q000821:virology; D006801:Humans; D014616:Vaccinia virus / Q000254:growth & development* / Q000302:isolation & purification*
chemical_list: D004279:DNA, Viral
keywords: cell culture; modified vaccinia virus Ankara; vaccinia virus