title: Telomerase-specific oncolytic adenovirus expressing TRAIL suppresses peritoneal dissemination of gastric cancer.
abstract: Peritoneal dissemination is the most common condition of metastasis in gastric cancer. The survival duration of a patient with advanced stage gastric cancer, may be improved by gene therapy. In this study, we used an oncolytic adenovirus vector (Ad/TRAIL-E1) that expresses both the TRAIL and E1A genes under the control of a tumor-specific promoter. We evaluated the anti-tumor effect of Ad/TRAIL-E1 on gastric cancer cells in vitro, as well as in vivo in a xenograft peritoneal carcinomatosis mouse model. Our data showed that Ad/TRAIL-E1 induced TRAIL-mediated apoptosis in gastric cancer cell lines, but not in the normal cell lines. In addition, Ad/TRAIL-E1 significantly inhibited peritoneal metastasis and prolonged the survival of mice without treatment-related toxicity. Therefore, tumor-specific TRAIL expression from an oncolytic adenovirus vector may provide a novel therapeutic approach for the treatment of advance stage gastric cancer with peritoneal dissemination.
authors.affiliation: Department of Colorectal Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Biotherapy of Zhejiang province, Hangzhou, China.
authors.forename: W
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Department of Colorectal Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Biotherapy of Zhejiang province, Hangzhou, China.
authors.forename: S
authors.initials: S
authors.lastname: Dai
authors.affiliation: Department of Colorectal Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Biotherapy of Zhejiang province, Hangzhou, China.
authors.forename: H
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Colorectal Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Biotherapy of Zhejiang province, Hangzhou, China.
authors.forename: Z
authors.initials: Z
authors.lastname: Song
authors.affiliation: Department of Biochemistry and Molecular Medicine, The George Washington University Medical School, Washington, DC, USA.
authors.forename: Y
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Biochemistry and Molecular Medicine, The George Washington University Medical School, Washington, DC, USA.
authors.forename: J B
authors.initials: JB
authors.lastname: Lee
authors.affiliation: Department of Biochemistry and Molecular Medicine, The George Washington University Medical School, Washington, DC, USA.
authors.forename: Z
authors.initials: Z
authors.lastname: Li
authors.affiliation: Key Laboratory of Biotherapy of Zhejiang province, Hangzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Hu
authors.affiliation: Department of Thoracic and Cardiovascular Surgery, The University of Texas M. D. Anderson Cancer Center, Houston, TX, USA.
authors.forename: B
authors.initials: B
authors.lastname: Fang
authors.affiliation: Department of Colorectal Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.|Key Laboratory of Biotherapy of Zhejiang province, Hangzhou, China.
authors.forename: C
authors.initials: C
authors.lastname: He
authors.affiliation: Department of Colorectal Surgery, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China.
authors.forename: X
authors.initials: X
authors.lastname: Huang
mesh_terms: D000256:Adenoviridae / Q000235:genetics; D017500:Adenovirus E1A Proteins / Q000235:genetics* / Q000627:therapeutic use; D000818:Animals; D017209:Apoptosis / Q000235:genetics; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000235:genetics; D015967:Gene Expression Regulation, Viral; D005822:Genetic Vectors; D006801:Humans; D051379:Mice; D050130:Oncolytic Virotherapy; D050504:Oncolytic Viruses / Q000235:genetics*; D010534:Peritoneal Neoplasms / Q000235:genetics / Q000556:secondary / Q000628:therapy* / Q000821:virology; D013274:Stomach Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy* / Q000821:virology; D053221:TNF-Related Apoptosis-Inducing Ligand / Q000235:genetics* / Q000627:therapeutic use; D019098:Telomerase / Q000235:genetics; D023041:Xenograft Model Antitumor Assays
chemical_list: D017500:Adenovirus E1A Proteins; D053221:TNF-Related Apoptosis-Inducing Ligand; C505653:TNFSF10 protein, human; D019098:Telomerase