title: A Novel Clerodane Diterpene from Vitex cofassus.
abstract: New clerodane diterpene, 16-hydroxy-pentandralactone (1) and known diterpene acuminolide (2) were isolated from the methanol extract of Vitex cofassus leaves. The chemical structure and the absolute configuration of 1 were determined by MS, NMR and electron circular dichroism (ECD) experiments. The isolated compounds were evaluated for their antiproliferative activities against a panel of human tumor cell lines, including a multidrug-resistant (MDR) cell line. Both compounds showed potent antiproliferative activities against all the tested cell lines with IC50values of 5.4-11.4 µM. Their effects on cell viability were also tested using vascular endothelial growth factor (VEGF)-stimulated human umbilical vein endothelial cells (HUVECs). Compound 1 inhibited VEGF-stimulated HUVEC proliferation in a dose-dependent manner. Based on these results, compound 1 could be a candidate for antitumor agent and inhibitor of angiogenesis.
authors.affiliation: School of Pharmaceutical Sciences, College of Medical, Pharmaceutical and Health Sciences, Kanazawa University.
authors.forename: Faradiba Abdul
authors.initials: FA
authors.lastname: Rasyid
authors.forename: Shuichi
authors.initials: S
authors.lastname: Fukuyoshi
authors.forename: Hirokazu
authors.initials: H
authors.lastname: Ando
authors.forename: Katsunori
authors.initials: K
authors.lastname: Miyake
authors.forename: Toshiyuki
authors.initials: T
authors.lastname: Atsumi
authors.forename: Tetsuo
authors.initials: T
authors.lastname: Fujie
authors.forename: Yohei
authors.initials: Y
authors.lastname: Saito
authors.forename: Masuo
authors.initials: M
authors.lastname: Goto
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Shinya
authors.forename: Masayuki
authors.initials: M
authors.lastname: Mikage
authors.forename: Yohei
authors.initials: Y
authors.lastname: Sasaki
authors.forename: Kyoko
authors.initials: K
authors.lastname: Nakagawa-Goto
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D045785:Diterpenes, Clerodane / Q000737:chemistry / Q000302:isolation & purification / Q000494:pharmacology*; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D061307:Human Umbilical Vein Endothelial Cells / Q000187:drug effects / Q000378:metabolism; D006801:Humans; D015394:Molecular Structure; D009389:Neovascularization, Pathologic / Q000188:drug therapy* / Q000473:pathology; D013329:Structure-Activity Relationship; D042442:Vascular Endothelial Growth Factors / Q000037:antagonists & inhibitors* / Q000378:metabolism; D020950:Vitex / Q000737:chemistry*
chemical_list: D000972:Antineoplastic Agents, Phytogenic; D045785:Diterpenes, Clerodane; D042442:Vascular Endothelial Growth Factors