title: Dataset of the proteome of purified outer membrane vesicles from the human pathogenAggregatibacter actinomycetemcomintans.
abstract: The Gram-negative bacteriumAggregatibacter actinomycetemcomitansis an oral and systemic pathogen, which is linked to aggressive forms of periodontitis and can be associated with endocarditis. The outer membrane vesicles (OMVs) of this species contain effector proteins such as cytolethal distending toxin (CDT) and leukotoxin (LtxA), which they can deliver into human host cells. The OMVs can also activate innate immunity through NOD1- and NOD2-active pathogen-associated molecular patterns. This dataset provides a proteome of highly purified OMVs fromA. actinomycetemcomitansserotypeestrain 173. The experimental data do not only include the raw data of the LC-MS/MS analysis of four independent preparations of purified OMVs but also the mass lists of the processed data and the Mascot.dat files from the database searches. In total 501 proteins are identified, of which 151 are detected in at least three of four independent preparations. In addition, this dataset contains the COG definitions and the predicted subcellular locations (PSORTb 3.0) for the entire genome ofA. actinomycetemcomitansserotypeestrain SC1083, which is used for the evaluation of the LC-MS/MS data. These data are deposited in ProteomeXchange in the public dataset PXD002509. In addition, a scientific interpretation of this dataset by Kieselbach et al. (2015) [2] is available at http://dx.doi.org/10.1371/journal.pone.0138591.
authors.affiliation: Department of Chemistry, Umeå University, Umeå, Sweden.
authors.forename: Thomas
authors.initials: T
authors.lastname: Kieselbach
authors.affiliation: Oral Microbiology, Department of Odontology, Umeå University, Umeå, Sweden.
authors.forename: Jan
authors.initials: J
authors.lastname: Oscarsson
keywords: Micobiology; Odontology; Outer membrane vesicle; Periodontitis; Proteomics