title: Antioxidant activity measurement and potential antioxidant peptides exploration from hydrolysates of novel continuous microwave-assisted enzymolysis of the Scomberomorus niphonius protein.
abstract: A novel continuous microwave-assisted enzymatic digestion (cMAED) method is proposed for the digestion of protein from Scomberomorus niphonius to obtain potential antioxidant peptides. In this study, bromelain was found to have a high capacity for the digestion of the Scomberomorus niphonius protein. The following cMAED conditions were investigated: protease species, microwave power, temperature, bromelain content, acidity of the substrate solution, and incubation time. At 400W, 40°C, 1500U·g-1bromelain, 20% substrate concentration, pH 6.0 and 5min incubation, the degree of hydrolysis and total antioxidant activity of the hydrolysates were 15.86% and 131.49μg·mL-1, respectively. The peptide analyses showed that eight of the potential antioxidant peptide sequences, which ranged from 502.32 to 1080.55Da with 4-10 amino acid residues, had features typical of well-known antioxidant proteins. Thus, the new cMAED method can be useful to obtain potential antioxidant peptides from protein sources, such as Scomberomorus niphonius.
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
authors.forename: Yipeng
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China. Electronic address: guihuaruan@hotmail.com.
authors.forename: Guihua
authors.initials: G
authors.lastname: Ruan
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China.
authors.forename: Zhijun
authors.initials: Z
authors.lastname: Qin
authors.affiliation: Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials, College of Chemistry and Bioengineering, Guilin University of Technology, Guangxi 541004, China. Electronic address: xglihaiyun@126.com.
authors.forename: Haiyun
authors.initials: H
authors.lastname: Li
authors.affiliation: Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518131, China.
authors.forename: Yanjie
authors.initials: Y
authors.lastname: Zheng
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects; D001963:Bromelains / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects; D029941:Fish Proteins / Q000032:analysis* / Q000378:metabolism; D005399:Fishes; D006868:Hydrolysis / Q000528:radiation effects; D008872:Microwaves; D010084:Oxidation-Reduction / Q000528:radiation effects; D010446:Peptide Fragments / Q000032:analysis* / Q000378:metabolism / Q000528:radiation effects
chemical_list: D000975:Antioxidants; D029941:Fish Proteins; D010446:Peptide Fragments; D001963:Bromelains; C091760:stem bromelain
keywords: Acrylamide (PubChem CID: 6579); Ammonium molybdate (PubChem CID: 61578); Antioxidant peptides; Ascorbic acid (PubChem CID: 54670067); Bromelain (PubChem CID: 44263865); Bromophenol blue (PubChem CID: 8272); Continuous microwave-assisted enzymatic digestion; Degree of hydrolysis; Disodium phosphate dodecahydrate (PubChem CID: 61456); Monopotassium phosphate (PubChem CID: 516951); Ninhydrin (PubChem CID: 10236); Peptide analysis; Sodium dodecyl sulfate (PubChem CID: 3423265); Sodium phosphate (PubChem CID: 24243); Total antioxidant activity