title: [MicroRNA-150 inhibits osteosarcoma cell proliferation by targeting RUNX2 gene].
abstract: To investigate the microRNA (miR)-150 expression level in human osteosarcoma cell lines (Saos-2, MG-63) and its function in cell proliferation, and to explore the potential molecular mechanisms.   Methods: MiR-150 expression levels in human osteosarcoma cell lines (Saos-2, MG-63) and normal osteoblast cell line (NHOst) were detected by relative quantitative real-time PCR (qRT-PCR). MiR-150 was overexpressed in Saos-2 and MG-63 cells by lentivirus infection. Cell proliferation rates were monitored by MTS assay. RUNX2 and β-actin protein levels were examined by Western blot. Inhibitory effect of miR-150 on binding RUNX2 3'-UTR was detected by Dual-Luciferase assay.  Results: MiR-150 expression level is lower in human osteosarcoma cell lines (Saos-2, MG-63) compared to the normal osteoblast cell line (NHOst) (0.23±0.02 and 0.32±0.03 vs 1.00±0.02), which showed statistical significance (P<0.01). After lentivirus infection, miR-150 level increased in Saos-2 (P<0.01) and MG-63 cells (P<0.01). Overexpression of miR-150 decreased cell proliferation and RUNX2 protein level in Saos-2 and MG-63 cells. The binding of miR-150 to RUNX2 3'-UTR decreased luciferase activity by 69% in Saos-2 cells (P<0.05) and 59% in MG-63 cells (P<0.05). Administration of exogenous RUNX2 recovered the cell proliferation in miR-150 overexpressed Saos-2 and MG-63 cell lines (P<0.01).  Conclusion: MiR-150 inhibites proliferation in human osteosarcoma cell lines through binding to RUNX2 3'-UTR, resulting in the reducion of RUNX2 protein level.
authors.affiliation: Department of Orthopaedic Trauma, Tianjin Hospital, Tianjin 300211, China.
authors.forename: Longfei
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Weiguo
authors.initials: W
authors.lastname: Wang
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Jinsong
authors.initials: J
authors.lastname: Li
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Shijie
authors.initials: S
authors.lastname: Chen
authors.affiliation: Department of Orthopedics, Third Xiangya Hospital, Central South University, Changsha 410013, China.
authors.forename: Ruisen
authors.initials: R
authors.lastname: Zhan
mesh_terms: D020413:3' Untranslated Regions; D000199:Actins / Q000378:metabolism; D001859:Bone Neoplasms; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics*; D050659:Core Binding Factor Alpha 1 Subunit / Q000187:drug effects* / Q000235:genetics / Q000494:pharmacology / Q000502:physiology*; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects* / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000494:pharmacology*; D010006:Osteoblasts / Q000502:physiology; D012516:Osteosarcoma / Q000235:genetics* / Q000503:physiopathology*
chemical_list: D020413:3' Untranslated Regions; D000199:Actins; D050659:Core Binding Factor Alpha 1 Subunit; D035683:MicroRNAs; C533097:MIR150, human; C493709:RUNX2 protein, human