title: Imaging, biodistribution and efficacy evaluation of188Re-human serum albumin microspheres via intraarterial route in an orthotopic hepatoma model.
abstract: Liver cancer is the second most common cause of death worldwide. This study was to investigate the SPECT/CT, ultrasound, biodistribution and therapeutic evaluation of188Re-human serum albumin microspheres (188Re-HSAM) in the GP7TB orthotopic hepatoma rat model.
  
  
  
  HSAM was labeled with188Re by using a home-made kit and microwave system. The188Re-HSAM was administered via intraarterial route. The in vivo distribution of188Re-HSAM was determined by biodistribution analysis and nanoSPECT/CT system. In efficacy, tumor volumes were tracked longitudinally by three-dimensional ultrasound.
  
  
  
  The biodistribution and nanoSPECT/CT imaging showed that188Re-HSAM could accumulate in liver and tumor. The correlation coefficient of tumor volumes done by three-dimensional ultrasound and at autopsy was 0.997. In efficacy, tumor volume in the normal saline-treated group was 1803.2 mm3at 54 days after tumor inoculation. Tumor volumes in the 103.6 MBq and 240.5 MBq of188Re-HSAM treated groups were 381 and 267.4 mm3(p = 0.001 and 0.004), respectively.
  
  
  
  These results show that three-dimensional ultrasound with a high spatial resolution and contrast in soft tissue can become imaging modality in assessing tumor burden and tumor progression in an orthotopic rat model. The longitudinally therapeutic evaluation of188Re-HSAM demonstrated dose-dependent tumor growth inhibition with increased dose in the GP7TB orthotopic hepatoma rat model.
authors.affiliation: a Department of Veterinary Medicine , National Chung Hsing University , Taichung , Taiwan.|b Isotope Application Division , Institute of Nuclear Energy Research , Taoyuan , Taiwan.
authors.forename: Liang-Cheng
authors.initials: LC
authors.lastname: Chen
authors.affiliation: b Isotope Application Division , Institute of Nuclear Energy Research , Taoyuan , Taiwan.
authors.forename: Ya-Jen
authors.initials: YJ
authors.lastname: Chang
authors.affiliation: b Isotope Application Division , Institute of Nuclear Energy Research , Taoyuan , Taiwan.
authors.forename: Su-Jung
authors.initials: SJ
authors.lastname: Chen
authors.affiliation: b Isotope Application Division , Institute of Nuclear Energy Research , Taoyuan , Taiwan.
authors.forename: Wan-Chi
authors.initials: WC
authors.lastname: Lee
authors.affiliation: b Isotope Application Division , Institute of Nuclear Energy Research , Taoyuan , Taiwan.
authors.forename: Chih-Hsien
authors.initials: CH
authors.lastname: Chang
authors.affiliation: b Isotope Application Division , Institute of Nuclear Energy Research , Taoyuan , Taiwan.
authors.forename: Te-Wei
authors.initials: TW
authors.lastname: Lee
authors.affiliation: a Department of Veterinary Medicine , National Chung Hsing University , Taichung , Taiwan.
authors.forename: Jui-Hung
authors.initials: JH
authors.lastname: Shien
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000528:radiation effects; D002214:Capsules / Q000138:chemical synthesis / Q000493:pharmacokinetics; D006528:Carcinoma, Hepatocellular / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000532:radiotherapy*; D045744:Cell Line, Tumor; D007269:Injections, Intra-Arterial; D008113:Liver Neoplasms / Q000000981:diagnostic imaging / Q000378:metabolism* / Q000532:radiotherapy*; D008297:Male; D009928:Organ Specificity; D011868:Radioisotopes / Q000008:administration & dosage* / Q000493:pharmacokinetics; D019275:Radiopharmaceuticals / Q000008:administration & dosage / Q000493:pharmacokinetics; D011916:Rats, Inbred F344; D012211:Rhenium / Q000008:administration & dosage* / Q000493:pharmacokinetics*; D012709:Serum Albumin / Q000737:chemistry; D000072098:Single Photon Emission Computed Tomography Computed Tomography; D000068936:Theranostic Nanomedicine; D014018:Tissue Distribution; D016896:Treatment Outcome; D047368:Tumor Burden / Q000528:radiation effects; D014463:Ultrasonography
chemical_list: D002214:Capsules; D011868:Radioisotopes; D019275:Radiopharmaceuticals; D012709:Serum Albumin; D012211:Rhenium
keywords: 188Re-HSAM; GP7TB orthotopic hepatoma model; SPECT/CT; intraarterial route; three-dimensional ultrasound