title: Clathrin-mediated endocytosis is involved in Tomato yellow leaf curl virus transport across the midgut barrier of its whitefly vector.
abstract: Tomato yellow leaf curl virus (TYLCV) is a begomovirus transmitted by the whitefly Bemisia tabaci. The circulative translocation of the virus in the insect is known in its broad line. However, transit of TYLCV from the digestive tract into the haemolymph is poorly understood. We studied the involvement of clathrin in this process by disrupting the clathrin-mediated endocytosis and the endosome network using inhibitor feeding, antibody blocking and dsRNA silencing. We monitored the quantities of TYLCV in the whitefly and virus transmission efficiency. Following endocytosis and endosome network disruption, the quantity of virus was higher in the midgut relative to that of the whole insect body, and the quantity of virus in the haemolymph was reduced. The transmission efficiency of TYLCV by the treated insects was also reduced. These findings indicate that clathrin-mediated endocytosis and endosomes play an important role in the transport of TYLCV across the whitefly midgut.
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Li-Long
authors.initials: LL
authors.lastname: Pan
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Qun-Fang
authors.initials: QF
authors.lastname: Chen
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Juan-Juan
authors.initials: JJ
authors.lastname: Zhao
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Guo
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China.
authors.forename: Xiao-Wei
authors.initials: XW
authors.lastname: Wang
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 10, Rehovot 76100, Israel.
authors.forename: Aliza
authors.initials: A
authors.lastname: Hariton-Shalev
authors.affiliation: Institute of Plant Sciences and Genetics in Agriculture, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O. Box 10, Rehovot 76100, Israel.
authors.forename: Henryk
authors.initials: H
authors.lastname: Czosnek
authors.affiliation: Ministry of Agriculture Key Laboratory of Agricultural Entomology, Institute of Insect Sciences, Zhejiang University, Hangzhou 310058, China. Electronic address: shshliu@zju.edu.cn.
authors.forename: Shu-Sheng
authors.initials: SS
authors.lastname: Liu
mesh_terms: D000818:Animals; D053839:Begomovirus / Q000502:physiology*; D002966:Clathrin / Q000378:metabolism*; D004705:Endocytosis; D011992:Endosomes / Q000378:metabolism / Q000821:virology; D041981:Gastrointestinal Tract / Q000378:metabolism / Q000821:virology; D006430:Hemiptera / Q000502:physiology / Q000821:virology*; D019476:Insect Proteins / Q000378:metabolism*; D007303:Insect Vectors / Q000502:physiology / Q000821:virology*; D018551:Solanum lycopersicum / Q000821:virology; D010935:Plant Diseases / Q000821:virology
chemical_list: D002966:Clathrin; D019476:Insect Proteins
keywords: Bemisia tabaci; Clathrin-mediated endocytosis; Digestive tract; Tomato yellow leaf curl virus; Virus transmission