title: In vitrobiocontrol activity of halotolerantStreptomyces aureofaciensK20: A potent antagonist againstMacrophomina phaseolina(Tassi) Goid.
abstract: A halotolerant actinobacterial strain isolated from salinity affected soil of Eastern Indo-Gangetic plains (IGP), Uttar Pradesh, India, was characterised for its antagonistic potential againstMacrophomina phaseolinaby dual-culture assay. It was shown to effectively inhibit the growth ofM. phaseolinawith an inhibition zone of 27 ± 1.33 mm. Further the actinobacterial strain was evaluated for its plant growth promoting (PGP) properties and its ability to produce biocontrol related extracellular enzymesviz.amylase, protease, cellulase, chitinase, gelatinase and urease. The results revealed that the actinobacterial strain had PGP potential along with positive assay for amylase, chitinase and urease. The interaction study between antagonist strain and fungal pathogen, performed by scanning electron microscopy technique revealed that the actinobacterium was able to damage fungal mycelia may be due to chitinase, establishing its role as a potential antagonist againstM. phaseolina. The actinobacterial isolate was characterised by 16S rDNA gene sequencing, and was identified asStreptomycesgenera. The identified gene sequence was deposited to NCBI GenBank with an accession number KP331758.
authors.affiliation: Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University (A Central Unversity), Lucknow 226025, Uttar Pradesh, India.
authors.forename: P
authors.initials: P
authors.lastname: Shrivastava
authors.affiliation: Department of Environmental Microbiology, Babasaheb Bhimrao Ambedkar University (A Central Unversity), Lucknow 226025, Uttar Pradesh, India.
authors.forename: R
authors.initials: R
authors.lastname: Kumar
authors.affiliation: National Bureau of Agricultural Insect Resources, H.A. Farm Post, Bellary Road, Bengaluru 560024, India.
authors.forename: M S
authors.initials: MS
authors.lastname: Yandigeri
keywords: Actinobacteria; Antagonism; Chitinase; Macrophomina phaseolina; PGP; Siderophore