title: A novel pathogenic aviadenovirus from red-bellied parrots (Poicephalus rufiventris) unveils deep recombination events among avian host lineages.
abstract: Competing roles of coevolution, selective pressure and recombination are an emerging interest in virus evolution. We report a novel aviadenovirus from captive red-bellied parrots (Poicephalus rufiventris) that uncovers evidence of deep recombination among aviadenoviruses. The sequence identity of the virus was most closely related to Turkey adenovirus D (42% similarity) and other adenoviruses in chickens, turkeys and pigeons. Sequencing and comparative analysis showed that the genome comprised 40,930 nucleotides containing 42 predicted open reading frames (ORFs) 19 of which had strong similarity with genes from other adenovirus species. The new genome unveiled a lineage that likely participated in deep recombination events across the genus Aviadenovirus accounting for an ancient evolutionary relationship. We hypothesize frequent host switch events and recombination among adenovirus progenitors in Galloanserae hosts caused the radiation of extant aviadenoviruses and the newly assembled Poicephalus adenovirus genome points to a potentially broader host range of these viruses among birds.
authors.affiliation: School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, New South Wales 2678, Australia. Electronic address: sdas@csu.edu.au.
authors.forename: Shubhagata
authors.initials: S
authors.lastname: Das
authors.affiliation: Hills District Veterinary Hospital, Unit 1, 276 New Line Road, Dural, NSW 2158, Australia. Electronic address: kathyfearnside@exemail.com.au.
authors.forename: Kathleen
authors.initials: K
authors.lastname: Fearnside
authors.affiliation: Department of Physiology, Anatomy and Microbiology, School of Life Sciences, La Trobe University, Melbourne, VIC 3086, Australia. Electronic address: S.Sarker@latrobe.edu.au.
authors.forename: Subir
authors.initials: S
authors.lastname: Sarker
authors.affiliation: School of Biomedical Sciences, Faculty of Science, Charles Sturt University, Wagga Wagga, NSW 2650, Australia. Electronic address: jforwood@csu.edu.au.
authors.forename: Jade K
authors.initials: JK
authors.lastname: Forwood
authors.affiliation: School of Animal and Veterinary Sciences, Faculty of Science, Charles Sturt University, New South Wales 2678, Australia. Electronic address: shraidal@csu.edu.au.
authors.forename: Shane R
authors.initials: SR
authors.lastname: Raidal
mesh_terms: D000257:Adenoviridae Infections / Q000662:veterinary* / Q000821:virology; D000818:Animals; D000259:Aviadenovirus / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification* / Q000502:physiology; D001715:Bird Diseases / Q000821:virology*; D001717:Birds / Q000145:classification / Q000821:virology; D016679:Genome, Viral; D058507:Host Specificity; D016366:Open Reading Frames; D010311:Parrots / Q000821:virology*; D010802:Phylogeny; D011995:Recombination, Genetic
keywords: Adenovirus; Aviadenovirus; Fowl adenovirus; Recombination