title: Dynamic imaging of allogeneic adipose-derived regenerative cells transplanted in ischemic hind limb of apolipoprotein E mouse model.
abstract: BACKGROUND
  Transplantation of allogeneic adipose-derived regenerative cells (ADRCs) is a promising treatment modality for severe ischemic diseases. However, minimal information is available on the in vivo effects, fate, and migration of ADRCs, as well as the mechanisms of their therapeutic angiogenesis.
  
  
  METHODS
  In this study, green fluorescent protein-expressing ADRCs (GFP-ADRCs) were obtained, labeled with acetylated 3-aminopropyltrimethoxysilane (APTS)-coated iron oxide nanoparticles (APTS NPs), and injected into an old apolipoprotein E knockout (ApoE-KO) mouse model with hind limb ischemia. Then, 3.0 T magnetic resonance imaging (MRI) was performed to dynamically trace the role of ADRCs targeting hind limb ischemia in the ApoE-KO mice model.
  
  
  RESULTS
  Labeled cells were visualized as large hypointense spots in ischemic muscles by serial 3.0 T MRI scans during a 4-week follow-up. The presence of labeled GFP-ADRCs was confirmed by Prussian blue staining and fluorescence microscopy on postmortem specimens.
  
  
  CONCLUSIONS
  This study showed that allogeneic ADRCs offer great potential application for therapeutic angiogenesis in severe ischemic disease based on the efficacy and feasibility of ADRC transplantation and on the available amounts of tissue.
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Guangxi.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Department of Vascular Surgery, School of Medicine, Shanghai Ninth People's Hospital Affiliated to Shanghai JiaoTong University.
authors.forename: Jinbao
authors.initials: J
authors.lastname: Qin
authors.affiliation: Department of Vascular Surgery, School of Medicine, Shanghai Ninth People's Hospital Affiliated to Shanghai JiaoTong University.
authors.forename: Xin
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Vascular Surgery, School of Medicine, Shanghai Ninth People's Hospital Affiliated to Shanghai JiaoTong University.
authors.forename: Zhiyou
authors.initials: Z
authors.lastname: Peng
authors.affiliation: Department of General Surgery, The Fourth Affiliated Hospital of Guangxi Medical University, Guangxi.
authors.forename: Peiyong
authors.initials: P
authors.lastname: Hou
authors.affiliation: Department of Vascular Surgery, School of Medicine, Shanghai Ninth People's Hospital Affiliated to Shanghai JiaoTong University; Vascular Center of Shanghai JiaoTong University, Shanghai, People's Republic of China.
authors.forename: Xinwu
authors.initials: X
authors.lastname: Lu
mesh_terms: D000273:Adipose Tissue / Q000166:cytology* / Q000637:transplantation*; D000818:Animals; D001057:Apolipoproteins E / Q000502:physiology*; D004195:Disease Models, Animal; D005260:Female; D006614:Hindlimb / Q000098:blood supply* / Q000473:pathology; D007091:Image Processing, Computer-Assisted; D007511:Ischemia / Q000503:physiopathology*; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D009389:Neovascularization, Pathologic / Q000517:prevention & control*; D012038:Regeneration / Q000502:physiology*
chemical_list: D001057:Apolipoproteins E
keywords: APTS nanoparticles; ApoE knockout mouse; allogeneic adipose-derived stem cells; cell tracking; hind limb ischemia