title: Effect of hydrogen peroxide on improving the heat stability of whey protein isolate solutions.
abstract: Whey protein isolate (WPI) solutions (12.8%w/w protein) were treated with varying concentrations of H2O2in the range of 0-0.144 H2O2to protein ratios (HTPR) by the addition of the required quantity of H2O2and deionized water. The samples were analyzed for heat stability, rheological properties, denaturation level of β-lactoglobulin (β-LG) and α-lactalbumin (α-LA). The samples treated with H2O2concentration >0.072 (HTPR) showed significant improvement in the heat stability, and decreased whey protein denaturation and aggregation. The WPI solution treated with H2O2(>0.072 HTPR) remained in the liquid state after heat treatment at 120°C, whereas the control samples formed gel upon heat treatment. Detailed analysis of these samples suggested that the improvement in the heat stability of H2O2treated WPI solution was attributed to the significant reduction in the sulfhydryl-disulfide interchange reaction during denaturation of β-LG and α-LA.
authors.affiliation: Dairy Science Department, South Dakota State University, Brookings, SD 57007, USA. Electronic address: sutariyasuresh83@gmail.com.
authors.forename: Suresh
authors.initials: S
authors.lastname: Sutariya
authors.affiliation: Dairy Science Department, South Dakota State University, Brookings, SD 57007, USA; Dairy Foods R&D, Land O'Lakes, Inc., Arden Hills, MN 55126, USA. Electronic address: HPatel@landolakes.com.
authors.forename: Hasmukh
authors.initials: H
authors.lastname: Patel
mesh_terms: D006358:Hot Temperature / Q000009:adverse effects*; D006861:Hydrogen Peroxide / Q000633:toxicity*; D006863:Hydrogen-Ion Concentration; D007768:Lactalbumin / Q000032:analysis / Q000378:metabolism; D007782:Lactoglobulins / Q000032:analysis / Q000378:metabolism; D008894:Milk Proteins / Q000032:analysis / Q000378:metabolism; D011489:Protein Denaturation / Q000187:drug effects; D055550:Protein Stability / Q000187:drug effects; D012212:Rheology / Q000379:methods; D012996:Solutions / Q000032:analysis / Q000378:metabolism; D000067816:Whey Proteins / Q000032:analysis* / Q000378:metabolism
chemical_list: D007782:Lactoglobulins; D008894:Milk Proteins; D012996:Solutions; D000067816:Whey Proteins; D007768:Lactalbumin; D006861:Hydrogen Peroxide
keywords: Gelation; Heat stability; Hydrogen peroxide; Whey protein; β-Lactoglobulin