title: Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.
abstract: The putrescine aminotransferase KES24511 from Pseudomonas sp. strain AAC was previously identified as an industrially relevant enzyme based on the discovery that it is able to promiscuously catalyse the transamination of 12-aminododecanoic acid. Here, the cloning, heterologous expression, purification and successful crystallization of the KES24511 protein are reported, which ultimately generated crystals adopting space group I2. The crystals diffracted X-rays to 2.07 Å resolution and data were collected using the microfocus beamline of the Australian Synchrotron. The structure was solved using molecular replacement, with a monomer from PDB entry 4a6t as the search model.
authors.affiliation: Land and Water, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Matthew
authors.initials: M
authors.lastname: Wilding
authors.affiliation: Land and Water, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Colin
authors.initials: C
authors.lastname: Scott
authors.affiliation: Biomedical Program, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
authors.affiliation: Biomedical Program, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Peat
mesh_terms: D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D007850:Lauric Acids / Q000737:chemistry* / Q000378:metabolism; D008958:Models, Molecular; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D011549:Pseudomonas / Q000737:chemistry* / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D000637:Transaminases / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D007850:Lauric Acids; D011994:Recombinant Proteins; C046049:12-aminododecanoic acid; D000637:Transaminases; C048018:diamine aminotransferase
keywords: 12-aminododecanoic acid; PLP-dependent; aminotransferases; transaminase