title: Anti-inflammatory effects of secondary metabolites isolated from the marine-derived fungal strain Penicillium sp. SF-5629.
abstract: After the chemical investigation of the ethyl acetate extract of the marine-derived fungal strain Penicillium sp. SF-5629, the isolation and structural elucidation of eight secondary metabolites, including (3R,4S)-6,8-dihydroxy-3,4,7-trimethylisocoumarin (1), (3S,4S)-sclerotinin A (2), penicitrinone A (3), citrinin H1 (4), emodin (5), ω-hydroxyemodin (6), 8-hydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (7), and 3,8-dihydroxy-6-methyl-9-oxo-9H-xanthene-1-carboxylate (8) were carried out. Evaluation of the anti-inflammatory activity of these metabolites showed that 4 inhibited nitric oxide and prostaglandin E2 production in lipopolysaccharide-stimulated BV2 microglia, with IC50values of 8.1 ± 1.9 and 8.0 ± 2.8 μM, respectively. The inhibitory function of 4 was confirmed based on decreases in inducible nitric oxide synthesis and cyclooxygenase-2 gene expression. In addition, 4 was found to suppress the phosphorylation of inhibitor kappa B-α, interrupt the nuclear translocation of nuclear factor kappa B, and decrease the activation of p38 mitogen-activated protein kinase.
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea.|Institute of Genome Research, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
authors.forename: Nguyen Thi Thanh
authors.initials: NT
authors.lastname: Ngan
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea.|Institute of Marine Biochemistry, Vietnam Academy of Science and Technology (VAST), 18 Hoang Quoc Viet, Caugiay, Hanoi, Vietnam.
authors.forename: Tran Hong
authors.initials: TH
authors.lastname: Quang
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea.
authors.forename: Kwan-Woo
authors.initials: KW
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea.
authors.forename: Hye Jin
authors.initials: HJ
authors.lastname: Kim
authors.affiliation: College of Medical and Life Sciences, Silla University, Busan, 46958, Republic of Korea.
authors.forename: Jae Hak
authors.initials: JH
authors.lastname: Sohn
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, 54538, Republic of Korea.
authors.forename: Dae Gill
authors.initials: DG
authors.lastname: Kang
authors.affiliation: Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, 54538, Republic of Korea.
authors.forename: Ho Sub
authors.initials: HS
authors.lastname: Lee
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea.|Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, 54538, Republic of Korea.
authors.forename: Youn-Chul
authors.initials: YC
authors.lastname: Kim
authors.affiliation: College of Pharmacy, Wonkwang University, Iksan, 54538, Republic of Korea. hoh@wonkwang.ac.kr.|Hanbang Body-Fluid Research Center, Wonkwang University, Iksan, 54538, Republic of Korea. hoh@wonkwang.ac.kr.
authors.forename: Hyuncheol
authors.initials: H
authors.lastname: Oh
mesh_terms: D000894:Anti-Inflammatory Agents, Non-Steroidal / Q000494:pharmacology*; D002460:Cell Line; D051546:Cyclooxygenase 2 / Q000096:biosynthesis / Q000235:genetics; D016207:Cytokines; D015232:Dinoprostone / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D006801:Humans; D017628:Microglia / Q000187:drug effects / Q000378:metabolism; D020928:Mitogen-Activated Protein Kinases / Q000378:metabolism; D016328:NF-kappa B / Q000187:drug effects / Q000378:metabolism; D009569:Nitric Oxide / Q000037:antagonists & inhibitors / Q000096:biosynthesis; D009792:Oceans and Seas; D010407:Penicillium / Q000737:chemistry*; D010766:Phosphorylation; D011485:Protein Binding
chemical_list: D000894:Anti-Inflammatory Agents, Non-Steroidal; D016207:Cytokines; D016328:NF-kappa B; D009569:Nitric Oxide; D051546:Cyclooxygenase 2; D020928:Mitogen-Activated Protein Kinases; D015232:Dinoprostone
keywords: Anti-inflammatory; Citrinin H1; Marine-derived fungus; Penicillium