title: Multiplexed detection of lung cancer biomarkers in patients serum with CMOS-compatible silicon nanowire arrays.
abstract: In this work, a real-time assay for highly sensitive, label-free, multiplexed electrical detection of lung cancer biomarkers was developed by using silicon nanowire field-effect (SiNW-FET) devices. Highly responsive SiNW arrays were fabricated using a CMOS-compatible anisotropic self-stop etching technique with mass reproducibility and low cost character. The SiNW nanosensor was integrated with PDMS microfluidic device, which allows rapid analyte delivery, makes the analysis to be conducted using exceedingly small samples and enables potential multiplexed detection. The nanowire arrays allowed highly selective and sensitive multiplexed detection of microRNA (miRNA)-126 and CEA. Due to high surface-to-volume ratio that the nanowire dimensions confer, the detection floor of single molecule was achieved. The potential utility in identifying clinical samples for early diagnosis of cancer was demonstrated by analyzing biomarkers in clinical related samples. The developed nanosensor with capability for multiplexed real-time monitoring of biomarkers with high sensitivity and selectivity in clinically relevant samples is highly attractive for diagnosis and treatment of cancer and other diseases.
authors.affiliation: Science and Technology on Micro-system Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Anran
authors.initials: A
authors.lastname: Gao
authors.affiliation: Science and Technology on Micro-system Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Xun
authors.initials: X
authors.lastname: Yang
authors.affiliation: Science and Technology on Micro-system Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Jing
authors.initials: J
authors.lastname: Tong
authors.affiliation: State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Lin
authors.initials: L
authors.lastname: Zhou
authors.affiliation: Science and Technology on Micro-system Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Yuelin
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China.
authors.forename: Jianlong
authors.initials: J
authors.lastname: Zhao
authors.affiliation: State Key Laboratories of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China. Electronic address: hjmao@mail.sim.ac.cn.
authors.forename: Hongju
authors.initials: H
authors.lastname: Mao
authors.affiliation: Science and Technology on Micro-system Laboratory, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, PR China. Electronic address: tli@mail.sim.ac.cn.
authors.forename: Tie
authors.initials: T
authors.lastname: Li
mesh_terms: D014408:Biomarkers, Tumor / Q000097:blood; D015374:Biosensing Techniques / Q000295:instrumentation*; D002272:Carcinoembryonic Antigen / Q000097:blood*; D004129:Dimethylpolysiloxanes / Q000737:chemistry; D004867:Equipment Design; D006801:Humans; D056656:Lab-On-A-Chip Devices; D008175:Lung Neoplasms / Q000097:blood*; D035683:MicroRNAs / Q000097:blood*; D053770:Nanowires / Q000737:chemistry* / Q000648:ultrastructure; D012825:Silicon / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D002272:Carcinoembryonic Antigen; D004129:Dimethylpolysiloxanes; C527111:MIRN126 microRNA, human; D035683:MicroRNAs; C013830:baysilon; D012825:Silicon
keywords: Multiplexed detection; PDMS microfluidic chip, lung cancer; SiNW-FET; Ultrasensitive