title: Expression Analysis of Genes Associated with Prolificacy in FecB Carrier and Noncarrier Indian Sheep.
abstract: The effect of FecB mutation on the gene expression in FecB carrier and noncarrier estrous synchronized ewes, has been analyzed. For this study the whole ovarian tissues and Graafian follicles were collected from estrus synchronized FecB carrier Garole, and non-carrier Deccani Indian sheep, showing remarkable differences in the numbers of preovulatory follicles among two groups. Eleven potential candidate genes (BMP15, GDF9, BMP4, BMP7, BMPR1B, BMPR1A, SMAD9, LHCGR, FSHR, IGF1R, and STAT5) were selected for their expression analysis by SybrGreen based real-time PCR, across ovaries and Graafian follicles of different fecundity groups, for having better insights into the effect of FecB genotypes on follicular development. Variable expression was observed for almost all the genes included in the present study among high and low fecundity groups that was most significant for the BMP7, BMP4, LHCGR, and FSHR transcripts in the ovarian follicles of high and low fecundity ewes, indicating their importance in governing the fecundity in FecB carrier, Indian Garole sheep. BMP4 expression among the genes studied was significantly higher in FecB carrier Garole sheep. This study confirms the changes in mRNA expression of the genes implicated in follicular development in FecB carrier and noncarrier Indian sheep breeds.
authors.affiliation: a Animal Biotechnology Division , National Bureau of Animal Genetic Resources , Karnal , Haryana , India.|b RIKEN Center for Life Science Technologies , Yokohama , Japan.
authors.forename: S
authors.initials: S
authors.lastname: Goyal
authors.affiliation: a Animal Biotechnology Division , National Bureau of Animal Genetic Resources , Karnal , Haryana , India.
authors.forename: J
authors.initials: J
authors.lastname: Aggarwal
authors.affiliation: a Animal Biotechnology Division , National Bureau of Animal Genetic Resources , Karnal , Haryana , India.|c Immune Regulation , WPI-IFREC, Osaka University , Osaka , Japan.
authors.forename: P K
authors.initials: PK
authors.lastname: Dubey
authors.affiliation: a Animal Biotechnology Division , National Bureau of Animal Genetic Resources , Karnal , Haryana , India.|d Indian Veterinary Research Institute , Izatnagar , Uttar Pradesh , India.
authors.forename: B P
authors.initials: BP
authors.lastname: Mishra
authors.affiliation: e Animal Husbandry Division , Nimbkar Agricultural Research Institute , Phaltan , Maharashtra , India.
authors.forename: P
authors.initials: P
authors.lastname: Ghalsasi
authors.affiliation: e Animal Husbandry Division , Nimbkar Agricultural Research Institute , Phaltan , Maharashtra , India.
authors.forename: C
authors.initials: C
authors.lastname: Nimbkar
authors.affiliation: a Animal Biotechnology Division , National Bureau of Animal Genetic Resources , Karnal , Haryana , India.
authors.forename: B K
authors.initials: BK
authors.lastname: Joshi
authors.affiliation: a Animal Biotechnology Division , National Bureau of Animal Genetic Resources , Karnal , Haryana , India.
authors.forename: R S
authors.initials: RS
authors.lastname: Kataria
mesh_terms: D000818:Animals; D052005:Bone Morphogenetic Protein Receptors, Type I / Q000235:genetics* / Q000378:metabolism; D005260:Female; D005298:Fertility / Q000235:genetics*; D020869:Gene Expression Profiling; D006080:Ovarian Follicle / Q000737:chemistry; D011967:Receptors, Gonadotropin / Q000235:genetics / Q000378:metabolism; D012756:Sheep / Q000235:genetics* / Q000502:physiology; D051785:Smad Proteins / Q000235:genetics / Q000378:metabolism; D016212:Transforming Growth Factor beta / Q000235:genetics / Q000378:metabolism
chemical_list: D011967:Receptors, Gonadotropin; D051785:Smad Proteins; D016212:Transforming Growth Factor beta; D052005:Bone Morphogenetic Protein Receptors, Type I
keywords: Differential expression; FecB carrier; fecundity genes; folliculogenesis; sheep