title: Evaluation of Alere i RSV for Rapid Detection of Respiratory Syncytial Virus in Children Hospitalized with Acute Respiratory Tract Infection.
abstract: Alere i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized with acute respiratory tract infection during the 2015-2016 winter season. Alere i RSV assay results were compared to those for Altona RealStar RSV real-time reverse transcription-PCR (RT-PCR). We found that the overall sensitivity and specificity of the Alere i RSV test was 100% (95% confidence intervals [CI], 93% to 100%) and 97% (95% CI, 89% to 100%), respectively. Positive samples were identified within 5 to 7 min from sample collection. Overall, the Alere i RSV test performed well compared to the RT-PCR assay and has the potential to facilitate the detection of RSV in point-of-care settings.
authors.affiliation: Center for Childhood and Adolescent Medicine (General Pediatrics), University Hospital Heidelberg, Heidelberg, Germany.|German Center for Infectious Diseases (DZIF), Heidelberg partner site, Heidelberg, Germany.|Center for Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany.
authors.forename: Rebecca Marie
authors.initials: RM
authors.lastname: Peters
authors.affiliation: Center for Childhood and Adolescent Medicine (General Pediatrics), University Hospital Heidelberg, Heidelberg, Germany.|German Center for Infectious Diseases (DZIF), Heidelberg partner site, Heidelberg, Germany.|Center for Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany.
authors.forename: Sarah Valerie
authors.initials: SV
authors.lastname: Schnee
authors.affiliation: Center for Childhood and Adolescent Medicine (General Pediatrics), University Hospital Heidelberg, Heidelberg, Germany.|German Center for Infectious Diseases (DZIF), Heidelberg partner site, Heidelberg, Germany.|Center for Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany.
authors.forename: Julia
authors.initials: J
authors.lastname: Tabatabai
authors.affiliation: Center for Infectious Diseases, Virology, University Hospital Heidelberg, Heidelberg, Germany.
authors.forename: Paul
authors.initials: P
authors.lastname: Schnitzler
authors.affiliation: Center for Childhood and Adolescent Medicine (General Pediatrics), University Hospital Heidelberg, Heidelberg, Germany Johannes.Pfeil@med.uni-heidelberg.de.
authors.forename: Johannes
authors.initials: J
authors.lastname: Pfeil
mesh_terms: D000293:Adolescent; D002648:Child; D002675:Child, Preschool; D005260:Female; D006760:Hospitalization; D006801:Humans; D007223:Infant; D008297:Male; D025202:Molecular Diagnostic Techniques / Q000379:methods*; D021141:Nucleic Acid Amplification Techniques / Q000379:methods*; D019095:Point-of-Care Systems; D011446:Prospective Studies; D012136:Respiratory Syncytial Viruses / Q000302:isolation & purification*; D012141:Respiratory Tract Infections / Q000175:diagnosis*; D012680:Sensitivity and Specificity; D013997:Time Factors
keywords: Alere i RSV; pediatric infectious disease; point-of-care; rapid tests; respiratory syncytial virus; respiratory tract infection