title: Detection of intermolecular transferred-NOE interactions in small and medium size protein complexes: RANTES complexed with a CCR5 N-terminal peptide.
abstract: NMR is a powerful tool for studying structural details of protein/peptide complexes exhibiting weak to medium binding (KD> 10 μm). However, it has been assumed that intermolecular nuclear Overhauser effect (NOE) interactions are difficult to observe in such complexes. We demonstrate that intermolecular NOEs can be revealed by combining the13C-edited/13C-filtered experiment with the transferred NOE effect (TRNOE). Due to the TRNOE phenomenon, intermolecular NOE cross peaks are characterized by both the chemical shifts (CSs) of the protein protons and the average CSs of the peptide protons, which are dominated by the CSs of the protons of the free peptide. Previously, the TRNOE phenomenon was used almost exclusively to investigate the conformation of small ligands bound to large biomolecules. Here, we demonstrate that TRNOE can be extended to enable the study of intermolecular interactions in small- and medium-sized protein complexes. We used the13C-edited/13C-filtered TRNOE experiment to study the interactions of the chemokine regulated upon activation, normal T cell, expressed and secreted (RANTES) with a 27-residue peptide, containing two sulfotyrosine residues, representing the N-terminal segment of the CCR5 receptor ((Nt-CCR5(1-27). The TRNOE phenomenon led to more than doubling of the signal-to-noise ratios (SNRs) for the intermolecular NOEs observed in the13C-edited/13C-filtered experiment for the 11.5-kDa monomeric RANTES/Nt-CCR5(1-27) complex. An even better improvement in the SNR was achieved with dimeric Nt-CCR5(1-27)/RANTES (23 kDa), especially in comparison with the spectra measured with a 1 : 1 protein to peptide ratio. In principle, the isotope-edited/isotope-filtered TRNOE spectrum can discern all intermolecular interactions involving nonexchangeable protons in the complex.
authors.affiliation: Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Meital
authors.initials: M
authors.lastname: Abayev
authors.affiliation: Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Gautam
authors.initials: G
authors.lastname: Srivastava
authors.affiliation: Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, NY, USA.|Department of Biochemistry and Chemistry, The Graduate Center of the City University of New York, NY, USA.
authors.forename: Boris
authors.initials: B
authors.lastname: Arshava
authors.affiliation: Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.|Department of Chemistry and Macromolecular Assembly Institute, College of Staten Island of the City University of New York, NY, USA.|Department of Biochemistry and Chemistry, The Graduate Center of the City University of New York, NY, USA.
authors.forename: Fred
authors.initials: F
authors.lastname: Naider
authors.affiliation: Department of Structural Biology, Weizmann Institute of Science, Rehovot, Israel.
authors.forename: Jacob
authors.initials: J
authors.lastname: Anglister
mesh_terms: D000595:Amino Acid Sequence; D001665:Binding Sites; D002247:Carbon Isotopes; D018946:Chemokine CCL5 / Q000737:chemistry* / Q000235:genetics; D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007700:Kinetics; D008970:Molecular Weight; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D011485:Protein Binding; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D019713:Receptors, CCR5 / Q000737:chemistry* / Q000235:genetics; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013194:Staining and Labeling / Q000379:methods
chemical_list: C483024:CCL5 protein, human; C584933:CCR5 protein, human; D002247:Carbon Isotopes; D018946:Chemokine CCL5; D019713:Receptors, CCR5; D011994:Recombinant Proteins
keywords: 
  NMR
  ; 
  NOE
  ; 
  RANTES
  ; 
  TRNOE
  ; CCR5; chemokines; intermolecular interactions; proteins