title: Hydrophilic Mesoporous Silica Materials for Highly Specific Enrichment of N-Linked Glycopeptide.
abstract: Hydrophilic interaction liquid chromatography (HILIC) is a significant enrichment strategy in glycoproteomics profiling. In this report, hydrophilic magnetic mesoporous silica materials (denoted as Fe3O4@mSiO2-IDA) were designed and synthesized as an outstanding enrichment platform for glycopeptide analysis. By taking advantage of their merits, such as large surface area, excellent hydrophilicity and unbiased affinity toward all types of glycopeptides, the Fe3O4@mSiO2-IDA nanomaterials were successfully applied to capture glycopeptides from complex samples. A total of 25 glycopeptides from horseradish peroxidase (HRP) digests and 33 glycopeptides from IgG were identified, respectively. Especially, as a result, 424 glycopeptides assigned to 140 glycoproteins were identified from only 2 μL human serum.
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University , Shanghai 200433, China.
authors.forename: Nianrong
authors.initials: N
authors.lastname: Sun
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University , Shanghai 200433, China.
authors.forename: Jiawen
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University , Shanghai 200433, China.
authors.forename: Jizong
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Chemistry and Institutes of Biomedical Sciences, Fudan University , Shanghai 200433, China.
authors.forename: Chunhui
authors.identifier: 0000-0002-8704-7543
authors.initials: C
authors.lastname: Deng
mesh_terms: D002853:Chromatography, Liquid; D052203:Ferrosoferric Oxide / Q000737:chemistry; D006020:Glycopeptides / Q000032:analysis* / Q000097:blood / Q000302:isolation & purification; D006735:Horseradish Peroxidase / Q000737:chemistry / Q000378:metabolism; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D007098:Imino Acids / Q000737:chemistry; D007074:Immunoglobulin G / Q000737:chemistry / Q000378:metabolism; D058185:Magnetite Nanoparticles / Q000737:chemistry; D049329:Nanostructures / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*
chemical_list: D006020:Glycopeptides; D007098:Imino Acids; D007074:Immunoglobulin G; D058185:Magnetite Nanoparticles; D012822:Silicon Dioxide; D006735:Horseradish Peroxidase; D052203:Ferrosoferric Oxide; C008109:iminodiacetic acid