title: Isotryptophan from Antarctic Bacillus amyloliquefaciens Pc3: purification, identification, characterization, and antifungal activity.
abstract: Strain Pc3 was isolated from Antarctic seawater and identified as Bacillus amyloliquefaciens. A compound with antifungal activity was purified from the fermentation supernatant of B. amyloliquefaciens Pc3. Its structure was determined to be (S)-2-amino-3-(1H-indol-2-yl) propanoic acid, named as isotryptophan, based on detailed analysis of its nuclear magnetic resonance (NMR) and high-resolution electrospray ionisation mass spectrometry (HR-ESI-MS). Isotryptophan exhibited good thermal stability and antifungal activity against several plant-pathogenic fungi with low minimum inhibitory concentrations. Therefore, the characterisation of isotryptophan from the Antarctic B. amyloliquefaciens Pc3 will facilitate its potential application in the control of plant-pathogenic fungal infection.
authors.affiliation: a Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography , State Oceanic Administration , Xiamen , P.R. China.
authors.forename: Pengfei
authors.initials: P
authors.lastname: Cui
authors.affiliation: a Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography , State Oceanic Administration , Xiamen , P.R. China.|b Key Laboratory of Marine Genetic Resources of Fujian Province , Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources , Xiamen , P.R. China.
authors.forename: Wenbin
authors.initials: W
authors.lastname: Guo
authors.affiliation: a Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography , State Oceanic Administration , Xiamen , P.R. China.|b Key Laboratory of Marine Genetic Resources of Fujian Province , Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources , Xiamen , P.R. China.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Chen
mesh_terms: D000864:Antarctic Regions; D000935:Antifungal Agents / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D000069977:Bacillus amyloliquefaciens / Q000737:chemistry*; D004353:Drug Evaluation, Preclinical / Q000379:methods; D009682:Magnetic Resonance Spectroscopy; D008826:Microbial Sensitivity Tests; D015394:Molecular Structure; D010944:Plants / Q000382:microbiology; D021241:Spectrometry, Mass, Electrospray Ionization; D014364:Tryptophan / Q000031:analogs & derivatives* / Q000737:chemistry / Q000494:pharmacology
chemical_list: D000935:Antifungal Agents; C000624328:isotryptophan; D014364:Tryptophan
keywords: Antarctic; Bacillus amyloliquefaciens; Isotryptophan; antifungal activity; plant-pathogenic fungi