title: Sulfatides Primarily Exist in the Substantia Nigra Region of Mouse Brain Tissue.
abstract: Lipid distribution in the brain is important for many biological functions and has been associated with some brain diseases. The aim of this study was to investigate lipid distribution in different regions of brain tissue in mice. To this end, substantia nigra (SN), caudate putamen (CPu), hippocampus (Hip), hypothalamus (Hyp), and cortex (Cx) tissues of mice were analyzed using direct infusion nanoelectrospray-ion trap mass spectrometry and multivariate analyses. The SN, CPu, Hip, Hyp, and Cx groups showed clear differences in lipid distribution using principal component analysis and a partial least-squares discriminant analysis score plot, and lipid levels were significantly different in different brain regions. In particular, sulfatides were mainly distributed in the SN region. Our results could be used to help understand the functions and mechanisms of lipids in various brain diseases.
authors.affiliation: BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea.
authors.forename: Kab-Tae
authors.initials: KT
authors.lastname: Park
authors.affiliation: BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea.
authors.forename: Jong Cheol
authors.initials: JC
authors.lastname: Shon
authors.affiliation: BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea.
authors.forename: Ji-Eun
authors.initials: JE
authors.lastname: Kim
authors.affiliation: College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea.
authors.forename: Gyu Hwan
authors.initials: GH
authors.lastname: Park
authors.affiliation: College of Pharmacy and Institute of Pharmaceutical Sciences, CHA University, Seongnam, 13488, Korea. hjchoi3@cha.ac.kr.
authors.forename: Hyun Jin
authors.initials: HJ
authors.lastname: Choi
authors.affiliation: BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea. dstlkh@knu.ac.kr.
authors.forename: Kwang-Hyeon
authors.initials: KH
authors.lastname: Liu
mesh_terms: D000818:Animals; D006624:Hippocampus / Q000378:metabolism; D007031:Hypothalamus / Q000378:metabolism; D008297:Male; D051379:Mice; D009928:Organ Specificity; D025341:Principal Component Analysis; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods*; D013378:Substantia Nigra / Q000378:metabolism*; D013433:Sulfoglycosphingolipids / Q000378:metabolism*; D014018:Tissue Distribution
chemical_list: D013433:Sulfoglycosphingolipids
keywords: Brain regions; Direct infusion nanoelectrospray-ion trap mass spectrometry; Substantia nigra; Sulfatides