title: Pex35 is a regulator of peroxisome abundance.
abstract: Peroxisomes are cellular organelles with vital functions in lipid, amino acid and redox metabolism. The cellular formation and dynamics of peroxisomes are governed byPEXgenes; however, the regulation of peroxisome abundance is still poorly understood. Here, we use a high-content microscopy screen inSaccharomyces cerevisiaeto identify new regulators of peroxisome size and abundance. Our screen led to the identification of a previously uncharacterized gene, which we termPEX35, which affects peroxisome abundance.PEX35encodes a peroxisomal membrane protein, a remote homolog to several curvature-generating human proteins. We systematically characterized the genetic and physical interactome as well as the metabolome of mutants inPEX35, and we found that Pex35 functionally interacts with the vesicle-budding-inducer Arf1. Our results highlight the functional interaction between peroxisomes and the secretory pathway.
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Ido
authors.initials: I
authors.lastname: Yofe
authors.affiliation: Department of Child and Adolescent Health, University Medical Center, Göttingen 37075, Germany.
authors.forename: Kareem
authors.initials: K
authors.lastname: Soliman
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Silvia G
authors.initials: SG
authors.lastname: Chuartzman
authors.affiliation: Department of Cellular Biochemistry, University of Kaiserslautern, Kaiserslautern 67653, Germany.|Division of Redox Regulation, ZMBH-DKFZ Alliance, German Cancer Research Center (DKFZ), Heidelberg 69121, Germany.
authors.forename: Bruce
authors.initials: B
authors.lastname: Morgan
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Uri
authors.initials: U
authors.lastname: Weill
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel.
authors.forename: Eden
authors.initials: E
authors.lastname: Yifrach
authors.affiliation: Division of Redox Regulation, ZMBH-DKFZ Alliance, German Cancer Research Center (DKFZ), Heidelberg 69121, Germany.
authors.forename: Tobias P
authors.initials: TP
authors.lastname: Dick
authors.affiliation: HudsonAlpha Institute for Biotechnology, Huntsville, AL 35806, USA.
authors.forename: Sara J
authors.initials: SJ
authors.lastname: Cooper
authors.affiliation: Department of Biochemistry and Molecular Biology, VILLUM Center for Bioanalytical Sciences, University of Southern Denmark, Odense 5230, Denmark.
authors.forename: Christer S
authors.initials: CS
authors.lastname: Ejsing
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel maya.schuldiner@weizmann.ac.il einat.zalckvar@weizmann.ac.il sven.thoms@med.uni-goettingen.de.
authors.forename: Maya
authors.initials: M
authors.lastname: Schuldiner
authors.affiliation: Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 7610001, Israel maya.schuldiner@weizmann.ac.il einat.zalckvar@weizmann.ac.il sven.thoms@med.uni-goettingen.de.
authors.forename: Einat
authors.initials: E
authors.lastname: Zalckvar
authors.affiliation: Department of Child and Adolescent Health, University Medical Center, Göttingen 37075, Germany maya.schuldiner@weizmann.ac.il einat.zalckvar@weizmann.ac.il sven.thoms@med.uni-goettingen.de.
authors.forename: Sven
authors.identifier: 0000-0003-3018-6363
authors.initials: S
authors.lastname: Thoms
mesh_terms: D004843:Epistasis, Genetic; D017353:Gene Deletion; D005800:Genes, Fungal; D008565:Membrane Proteins / Q000378:metabolism*; D008853:Microscopy; D020675:Peroxisomes / Q000378:metabolism*; D012441:Saccharomyces cerevisiae / Q000235:genetics / Q000378:metabolism*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*
chemical_list: D008565:Membrane Proteins; C000621741:Pex35 protein, S cerevisiae; D029701:Saccharomyces cerevisiae Proteins
keywords: Arf1; High-content screen; Peroxisomes; Pex35; Yeast; Ygr168c