title: Evaluation of the phototoxicity of unsubstituted and alkylated polycyclic aromatic hydrocarbons to mysid shrimp (Americamysis bahia): Validation of predictive models.
abstract: Crude oils are composed of an assortment of hydrocarbons, some of which are polycyclic aromatic hydrocarbons (PAHs). Polycyclic aromatic hydrocarbons are of particular interest due to their narcotic and potential phototoxic effects. Several studies have examined the phototoxicity of individual PAHs and fresh and weathered crude oils, and several models have been developed to predict PAH toxicity. Fingerprint analyses of oils have shown that PAHs in crude oils are predominantly alkylated. However, current models for estimating PAH phototoxicity assume toxic equivalence between unsubstituted (i.e., parent) and alkyl-substituted compounds. This approach may be incorrect if substantial differences in toxic potency exist between unsubstituted and substituted PAHs. The objective of the present study was to examine the narcotic and photo-enhanced toxicity of commercially available unsubstituted and alkylated PAHs to mysid shrimp (Americamysis bahia). Data were used to validate predictive models of phototoxicity based on the highest occupied molecular orbital-lowest unoccupied molecular orbital (HOMO-LUMO) gap approach and to develop relative effect potencies. Results demonstrated that photo-enhanced toxicity increased with increasing methylation and that phototoxic PAH potencies vary significantly among unsubstituted compounds. Overall, predictive models based on the HOMO-LUMO gap were relatively accurate in predicting phototoxicity for unsubstituted PAHs but are limited to qualitative assessments. Environ Toxicol Chem 2017;36:2043-2049. © 2017 SETAC.
authors.affiliation: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon, USA.
authors.forename: Bryson E
authors.initials: BE
authors.lastname: Finch
authors.affiliation: Department of Civil and Environmental Engineering, University of Delaware, Newark, Delaware, USA.
authors.forename: Solmaz
authors.initials: S
authors.lastname: Marzooghi
authors.affiliation: Department of Civil and Environmental Engineering, University of Delaware, Newark, Delaware, USA.
authors.forename: Dominic M
authors.initials: DM
authors.lastname: Di Toro
authors.affiliation: Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, Oregon, USA.
authors.forename: William A
authors.initials: WA
authors.lastname: Stubblefield
mesh_terms: D000478:Alkylation; D000818:Animals; D003445:Crustacea / Q000187:drug effects* / Q000528:radiation effects; D004784:Environmental Monitoring; D007928:Lethal Dose 50; D008027:Light / Q000009:adverse effects; D008962:Models, Theoretical; D019055:No-Observed-Adverse-Effect Level; D010578:Petroleum / Q000633:toxicity*; D011084:Polycyclic Aromatic Hydrocarbons / Q000737:chemistry / Q000633:toxicity*; D013329:Structure-Activity Relationship; D053608:Stupor / Q000139:chemically induced; D016019:Survival Analysis; D014466:Ultraviolet Rays / Q000009:adverse effects*
chemical_list: D010578:Petroleum; D011084:Polycyclic Aromatic Hydrocarbons
keywords: Narcotic toxicity; Phototoxicity; Polycyclic aromatic hydrocarbons (PAHs); Predictive model; Relative effect potency