title: Effect of pore-size optimization on the performance of polysaccharide-based superficially porous chiral stationary phases for the separation of enantiomers in high-performance liquid chromatography.
abstract: Our earlier studies on the preparation of chiral stationary phases (CSP) based on superficially porous (or core-shell) silica (SPS) particles for the separation of enantiomers in HPLC have provided proof to the advantages of such sorbents. In particular, higher enantioselectivity was observed with the columns packed with superficially porous CSP compared to the columns packed with fully-porous (FP) silica-based CSPs at comparable content of chiral selector (polysaccharide derivative) in CSP. Also, less dependence of plate height on mobile phase flow rate and higher plate numbers and resolution calculated per unit time (i.e. speed of separation) were observed with SPS-based CSPs. Thirty years of CSP development have demonstrated that wide-pore silica has to be used as a support for large molecular weight chiral selectors such as the ones based on polysaccharides. In this study the effect of pore size of the core-shell silica support and of other experimental factors on column performance is demonstrated. Reduced plate heights in the range 1.4-1.5 were obtained, as well as highly effective baseline separations of enantiomers were observed with analysis times of less than 15s.
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
authors.forename: Lia
authors.initials: L
authors.lastname: Bezhitashvili
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
authors.forename: Anna
authors.initials: A
authors.lastname: Bardavelidze
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
authors.forename: Teona
authors.initials: T
authors.lastname: Ordjonikidze
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia.
authors.forename: Lali
authors.initials: L
authors.lastname: Chankvetadze
authors.affiliation: Phenomenex Inc., 411 Madrid Ave., Torrance, 90501 CA, USA.
authors.forename: Mike
authors.initials: M
authors.lastname: Chity
authors.affiliation: Phenomenex Inc., 411 Madrid Ave., Torrance, 90501 CA, USA.
authors.forename: Tivadar
authors.initials: T
authors.lastname: Farkas
authors.affiliation: Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Chavchavadze Ave 3, 0179 Tbilisi, Georgia. Electronic address: jpba_bezhan@yahoo.com.
authors.forename: Bezhan
authors.initials: B
authors.lastname: Chankvetadze
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000295:instrumentation / Q000379:methods*; D008970:Molecular Weight; D011134:Polysaccharides / Q000737:chemistry*; D016062:Porosity; D012822:Silicon Dioxide / Q000737:chemistry*; D013237:Stereoisomerism
chemical_list: D011134:Polysaccharides; D012822:Silicon Dioxide
keywords: Chiral HPLC; Chiral stationary phases based on superficially porous silica; Enantioseparations; Polysaccharide-based chiral stationary phases