title: Real-time measurement of small molecules directly in awake, ambulatory animals.
abstract: The development of a technology capable of tracking the levels of drugs, metabolites, and biomarkers in the body continuously and in real time would advance our understanding of health and our ability to detect and treat disease. It would, for example, enable therapies guided by high-resolution, patient-specific pharmacokinetics (including feedback-controlled drug delivery), opening new dimensions in personalized medicine. In response, we demonstrate here the ability of electrochemical aptamer-based (E-AB) sensors to support continuous, real-time, multihour measurements when emplaced directly in the circulatory systems of living animals. Specifically, we have used E-AB sensors to perform the multihour, real-time measurement of four drugs in the bloodstream of even awake, ambulatory rats, achieving precise molecular measurements at clinically relevant detection limits and high (3 s) temporal resolution, attributes suggesting that the approach could provide an important window into the study of physiology and pharmacokinetics.
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106.|Center for Bioengineering, University of California, Santa Barbara, CA 93106.
authors.forename: Netzahualcóyotl
authors.identifier: 0000-0002-2740-6276
authors.initials: N
authors.lastname: Arroyo-Currás
authors.affiliation: Center for Bioengineering, University of California, Santa Barbara, CA 93106.|Interdepartmental Program in Biomolecular Science and Engineering, University of California, Santa Barbara, CA 93106.
authors.forename: Jacob
authors.initials: J
authors.lastname: Somerson
authors.affiliation: Department of Psychology, California State University, Dominguez Hills, Carson, CA 90747.
authors.forename: Philip A
authors.initials: PA
authors.lastname: Vieira
authors.affiliation: Department of Psychological and Brain Sciences, University of California, Santa Barbara, CA 93106.
authors.forename: Kyle L
authors.initials: KL
authors.lastname: Ploense
authors.affiliation: Department of Psychological and Brain Sciences, University of California, Santa Barbara, CA 93106.|The Neuroscience Research Institute, University of California, Santa Barbara, CA 93106.|Department of Molecular Cellular and Developmental Biology, University of California, Santa Barbara, CA 93106.
authors.forename: Tod E
authors.initials: TE
authors.lastname: Kippin
authors.affiliation: Department of Chemistry and Biochemistry, University of California, Santa Barbara, CA 93106; kwp@chem.ucsb.edu.|Center for Bioengineering, University of California, Santa Barbara, CA 93106.|Interdepartmental Program in Biomolecular Science and Engineering, University of California, Santa Barbara, CA 93106.
authors.forename: Kevin W
authors.initials: KW
authors.lastname: Plaxco
mesh_terms: D000818:Animals; D052157:Aptamers, Nucleotide / Q000378:metabolism; D015374:Biosensing Techniques / Q000379:methods; D002417:Cattle; D006801:Humans; D057230:Limit of Detection; D008297:Male; D004364:Pharmaceutical Preparations / Q000097:blood* / Q000378:metabolism*; D051381:Rats; D017207:Rats, Sprague-Dawley; D054852:Small Molecule Libraries / Q000378:metabolism*
chemical_list: D052157:Aptamers, Nucleotide; D004364:Pharmaceutical Preparations; D054852:Small Molecule Libraries
keywords: E-DNA; aptamer; in vivo; precision medicine; square-wave voltammetry