title: Prognostic value of microRNAs in osteosarcoma: A meta-analysis.
abstract: BACKGROUND
  Osteosarcoma is the most common primary bone malignancy. We meta-analyzed the prognostic value of altered miRNAs in patients with osteosarcoma.
  
  
  METHODS
  Sources from MEDLINE (from inception to August 2016) and EMBASE (from inception to August 2016) were searched. Studies of osteosarcoma with results of miRNA and studies that reported survival data were included and two authors performed the data extraction independently. Any discrepancies were resolved by a consensus. The outcome was overall survival and event-free survival assessed using hazard ratios (HRs).
  
  
  RESULTS
  After reviewing the full text of 65 articles, 25 studies including 2,278 patients were eligible in this study. The pooled HR for deaths was 1.40 (95% confidence interval [CI] 1.01-1.94, p=0.04) with random-effects model (χ2=113.08, p<0.00001, I2=79%) for patients of osteosarcoma with lower expression of miRNA. However, the pooled HR for events was not significant (HR 0.97, 0.63-1.48, p=0.87, χ2=72.65, p<0.00001, I2=79%). In pathway analysis of miRNAs, miRNA449a, 199-5p, 542-5p have common target genes.
  
  
  CONCLUSIONS
  Expression level of miRNA in patients of osteosarcoma is important as a prognostic factor.
authors.affiliation: BEER, Busan Society of Evidence-Based Medicine and Research, Busan, Republic of Korea.|Department of Anatomy, School of Medicine, Pusan National University, Yangsan, Gyeongnam, Republic of Korea.
authors.forename: Yun Hak
authors.initials: YH
authors.lastname: Kim
authors.affiliation: BEER, Busan Society of Evidence-Based Medicine and Research, Busan, Republic of Korea.|Department of Orthopaedic Surgery and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
authors.forename: Tae Sik
authors.initials: TS
authors.lastname: Goh
authors.affiliation: Biomedical Research Institute, Pusan National University Hospital and School of Medicine, Pusan National University, Busan, Republic of Korea.
authors.forename: Chi-Seung
authors.initials: CS
authors.lastname: Lee
authors.affiliation: Department of Anatomy, School of Medicine, Pusan National University, Yangsan, Gyeongnam, Republic of Korea.
authors.forename: Sae Ock
authors.initials: SO
authors.lastname: Oh
authors.affiliation: Department of Orthopaedic Surgery and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
authors.forename: Jeung Il
authors.initials: JI
authors.lastname: Kim
authors.affiliation: Department of Orthopaedic Surgery and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
authors.forename: Seung Hyeon
authors.initials: SH
authors.lastname: Jeung
authors.affiliation: BEER, Busan Society of Evidence-Based Medicine and Research, Busan, Republic of Korea.|Department of Nuclear Medicine and Biomedical Research Institute, Pusan National University Hospital, Busan, Republic of Korea.
authors.forename: Kyoungjune
authors.initials: K
authors.lastname: Pak
mesh_terms: D014408:Biomarkers, Tumor / Q000235:genetics*; D001859:Bone Neoplasms / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D016009:Chi-Square Distribution; D018450:Disease Progression; D018572:Disease-Free Survival; D020022:Genetic Predisposition to Disease; D006801:Humans; D053208:Kaplan-Meier Estimate; D035683:MicroRNAs / Q000235:genetics*; D016017:Odds Ratio; D012516:Osteosarcoma / Q000235:genetics* / Q000401:mortality / Q000473:pathology / Q000628:therapy; D010641:Phenotype; D018570:Risk Assessment; D012307:Risk Factors; D013997:Time Factors; D016896:Treatment Outcome
chemical_list: D014408:Biomarkers, Tumor; D035683:MicroRNAs
keywords: meta-analysis; microRNA; osteosarcoma; prognosis