title: Use of Membrane Potential to Achieve Transmembrane Modification with an Artificial Receptor.
abstract: We developed a strategy to modify cell membranes with an artificial transmembrane receptor. Coulomb force on the receptor, caused by the membrane potential, was used to achieve membrane penetration. A hydrophobically modified cationic peptide was used as a membrane potential sensitive region that was connected to biotin through a transmembrane oligoethylene glycol (OEG) chain. This artificial receptor gradually disappeared from the cell membrane via penetration despite the presence of a hydrophilic OEG chain. However, when the receptor was bound to streptavidin (SA), it remained on the cell membrane because of the large and hydrophilic nature of SA.
authors.forename: Wataru
authors.initials: W
authors.lastname: Hatanaka
authors.forename: Miki
authors.initials: M
authors.lastname: Kawaguchi
authors.forename: Xizheng
authors.initials: X
authors.lastname: Sun
authors.forename: Yusuke
authors.initials: Y
authors.lastname: Nagao
authors.affiliation: Faculty of Pharmaceutical Sciences, Tokyo University of Science , 2641 Yamazaki, Noda, Chiba 278-8501, Japan.
authors.forename: Hiroyuki
authors.initials: H
authors.lastname: Ohshima
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University , Kyoto 606-8501, Japan.
authors.forename: Mitsuru
authors.initials: M
authors.lastname: Hashida
authors.affiliation: Graduate School of Pharmaceutical Sciences, Kyoto University , Kyoto 606-8501, Japan.
authors.forename: Yuriko
authors.initials: Y
authors.lastname: Higuchi
authors.forename: Akihiro
authors.identifier: 0000-0002-0503-1418
authors.initials: A
authors.lastname: Kishimura
authors.forename: Yoshiki
authors.initials: Y
authors.lastname: Katayama
authors.forename: Takeshi
authors.identifier: 0000-0002-1821-5427
authors.initials: T
authors.lastname: Mori
mesh_terms: D002462:Cell Membrane / Q000378:metabolism*; D006801:Humans; D057927:Hydrophobic and Hydrophilic Interactions; D042541:Intracellular Space / Q000378:metabolism; D020014:K562 Cells; D008564:Membrane Potentials; D011092:Polyethylene Glycols / Q000737:chemistry; D062165:Receptors, Artificial / Q000737:chemistry / Q000378:metabolism*; D012995:Solubility; D019809:Streptavidin / Q000378:metabolism; D014867:Water / Q000737:chemistry
chemical_list: D062165:Receptors, Artificial; D014867:Water; D011092:Polyethylene Glycols; D019809:Streptavidin