title: Retrospective growth kinetics and radiosensitivity analysis of various human xenograft models.
abstract: The purpose of this study was to delineate the various factors that affect the growth characteristics of human cancer xenografts in nude mice and to reveal the relationship between the growth characteristics and radiosensitivity. We retrospectively analyzed 390 xenografts comprising nine different human cancer lines grown in nude mice used in our institute between 2009 and 2015. Tumor growth rate (TGR) was calculated using exponential growth equations. The relationship between the TGR of xenografts and the proliferation of the cellsin vitrowas examined. Additionally, we examined the correlations between the surviving fractions of cells after 2 Gy irradiationin vitroand the response of the xenograft to radiation. The TGR of xenografts was positively related to the proliferation of the cellsin vitro(rP=0.9714,p<0.0001), whereas it was independent of the histological type of the xenografts. Radiation-induced suppression of the growth rate (T/C%) of xenografts was positively related to the radiosensitivity of the cellsin vitro(SF2;rP=0.8684,p=0.0284) and TGR (rP=0.7623,p=0.0780). The proliferation of human cancer cellsin vitroand the growth rate of xenografts were positively related. The radiosensitivity of cancer cells, as judged from the SF2valuesin vitro, and the radiation-induced suppression of xenograft growth were positively related. In conclusion, the growth rate of human xenografts was independent of histological type and origin of the cancer cells, and was positively related to the proliferation of the cancer cellsin vitro.
authors.affiliation: Radiation Non-clinical Center, Seoul, Korea.; Department of Biosystems Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors.forename: Ji Young
authors.initials: JY
authors.lastname: Lee
authors.affiliation: Department of Radiation Oncology, Seoul, Korea.
authors.forename: Mi-Sook
authors.initials: MS
authors.lastname: Kim
authors.affiliation: Division of Heavy Ion Clinical Research, Korea Institute of Radiological and Medical Sciences, Seoul, Korea.
authors.forename: Eun Ho
authors.initials: EH
authors.lastname: Kim
authors.affiliation: Department of Biosystems Engineering, College of Life Sciences and Biotechnology, Korea University, Seoul, Korea.
authors.forename: Namhyun
authors.initials: N
authors.lastname: Chung
authors.affiliation: Radiation Non-clinical Center, Seoul, Korea.
authors.forename: Youn Kyoung
authors.initials: YK
authors.lastname: Jeong
keywords: Human xenograft model; cell line characteristics; in vitro-in vivo correlation; radiation; retrospective analysis