title: Wettability and surface morphology of eroded dentin treated with chitosan.
abstract: OBJECTIVE
  To assess the effect of chitosan, at concentrations of 2.5% and 5.0%, on the wettability of the eroded dentin, followed by analysis of surface morphology by SEM.
  
  
  METHODS
  104 bovine dentin slabs were ground, polished and then immersed in 20mL of citric acid (pH=3.2) under continuous stirring for 2h. Specimens were randomly divided according to the dentin substrate: sound and eroded, and then, subdivided into 4 groups (n=10): without rewetting (control), 1% acetic acid, 2.5% chitosan and 5.0% chitosan. Then, a drop of the adhesive system Single Bond 2 (3M) was deposited onto surface of each specimen. The contact angle between dentin surface and the adhesive system was measured by using a goniometer. The other 24 specimens were subjected to analysis under SEM. Statistical analysis was performed using the normality test (Kolmogorov-Smirnov) and Analysis of Variance (ANOVA) (p>0.05).
  
  
  RESULTS
  No differences were found between the angles produced on the eroded dentin rewetting with chitosan at the concentrations of 2.5% and 5%.
  
  
  CONCLUSIONS
  The chitosan, regardless of the concentration used, did not influence the eroded dentin wettability. Through SEM analysis, it was found particles of chitosan deposited on the surface and within the dentinal tubules.
authors.affiliation: Department of Restorative Dentistry, Sao Paulo University, Café Avenue, Monte Alegre, Ribeirão Preto, SP, Brazil.
authors.forename: Mirian Saavedra
authors.initials: MS
authors.lastname: Ururahy
authors.affiliation: Department of Restorative Dentistry, Sao Paulo University, Café Avenue, Monte Alegre, Ribeirão Preto, SP, Brazil.
authors.forename: Fabiana Almeida
authors.initials: FA
authors.lastname: Curylofo-Zotti
authors.affiliation: Department of Dentistry, Federal University of Jequitinhonha and Mucuri Valleys, Diamantina, MG, Brazil.
authors.forename: Rodrigo
authors.initials: R
authors.lastname: Galo
authors.affiliation: Department of Chemistry, Sao Paulo University, Bandeirantes Avenue, Ribeirao Preto, SP, Brazil.
authors.forename: Lucas Fabricio Bahia
authors.initials: LF
authors.lastname: Nogueira
authors.affiliation: Department of Chemistry, Sao Paulo University, Bandeirantes Avenue, Ribeirao Preto, SP, Brazil.
authors.forename: Ana Paula
authors.initials: AP
authors.lastname: Ramos
authors.affiliation: Department of Restorative Dentistry, Sao Paulo University, Café Avenue, Monte Alegre, Ribeirão Preto, SP, Brazil. Electronic address: silmaracorona@forp.usp.br.
authors.forename: Silmara Aparecida Milori
authors.initials: SA
authors.lastname: Corona
mesh_terms: D019342:Acetic Acid; D000704:Analysis of Variance; D000818:Animals; D017438:Bisphenol A-Glycidyl Methacrylate; D002417:Cattle; D048271:Chitosan / Q000494:pharmacology*; D019343:Citric Acid; D001840:Dental Bonding; D003804:Dentin / Q000737:chemistry* / Q000000981:diagnostic imaging / Q000473:pathology; D017220:Dentin-Bonding Agents / Q000737:chemistry; D007180:Incisor / Q000187:drug effects; D008855:Microscopy, Electron, Scanning; D014077:Tooth Erosion / Q000139:chemically induced / Q000000981:diagnostic imaging / Q000473:pathology*; D017155:Wettability / Q000187:drug effects
chemical_list: D017220:Dentin-Bonding Agents; C115612:single bond; D019343:Citric Acid; D017438:Bisphenol A-Glycidyl Methacrylate; D048271:Chitosan; D019342:Acetic Acid
keywords: Chitosan; Contact angle; Eroded dentin; Wettability