title: Endogenous Hydrogen Sulfide Homeostasis Is Responsible for the Alleviation of Senescence of Postharvest Daylily Flower via Increasing Antioxidant Capacity and Maintained Energy Status.
abstract: There are limited data concerning the role of endogenous H2S in prolonging the postharvest of vegetables and fruits. Using a fluorescence microscope with a specific probe, we discovered that, during the senescence of postharvest daylily flower, endogenous H2S homeostasis was impaired. The activities of two important synthetic enzymes of H2S, l- and d-cysteine desulfhydrase, exhibited decreasing tendencies. However, NaHS (a H2S donor) not only blocked the decreased H2S production but also extended the postharvest life of daylilies. These beneficial roles were verified by the alleviation of lipid peroxidation and the increased activities of antioxidant enzymes. Meanwhile, the energy status was sustained, and the respiration rate was decreased. In contrast to NaHS, the addition of an inhibitor of H2S synthesis alone aggravated lipid peroxidation and lowered energy charge. Together, the present study implies that endogenous H2S alleviates senescence of postharvest daylilies via increasing antioxidant capacity and maintained energy status.
authors.affiliation: College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University , Nanjing 210095, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Liu
authors.affiliation: Institute of Botany, Jiangsu Province and Chinese Academy of Sciences , Nanjing 210014, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Xu
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences , Nanjing 210014, China.
authors.forename: Huali
authors.initials: H
authors.lastname: Hu
authors.affiliation: College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University , Nanjing 210095, China.
authors.forename: Jincheng
authors.initials: J
authors.lastname: Pan
authors.affiliation: Institute of Agricultural Products Processing, Jiangsu Academy of Agricultural Sciences , Nanjing 210014, China.
authors.forename: Pengxia
authors.initials: P
authors.lastname: Li
authors.affiliation: College of Life Sciences, Laboratory Center of Life Sciences, Nanjing Agricultural University , Nanjing 210095, China.
authors.forename: Wenbiao
authors.identifier: http://orcid.org/0000-0003-1525-9472
authors.initials: W
authors.lastname: Shen
mesh_terms: D000975:Antioxidants / Q000378:metabolism*; D004734:Energy Metabolism; D035264:Flowers / Q000254:growth & development* / Q000378:metabolism; D031421:Hemerocallis / Q000254:growth & development / Q000378:metabolism*; D006706:Homeostasis; D006862:Hydrogen Sulfide / Q000378:metabolism*; D015227:Lipid Peroxidation
chemical_list: D000975:Antioxidants; D006862:Hydrogen Sulfide
keywords: antioxidant capacity; daylily; endogenous hydrogen sulfide homeostasis; energy status; senescence