title: Phenylketonuria is not a risk factor for changes of inflammation status as assessed by interleukin 6 and interleukin 8 concentrations.
abstract: BACKGROUND
  High oxidative stress and a reduced potential for free radical scavenging in phenylketonuria (PKU) patients, a phenomenon confirmed in a few studies, may lead to systemic chronic inflammation. The aim of this study was to compare the inflammation status, as assessed by interleukin 6 and interleukin 8 concentrations, in patients with PKU and in healthy controls.
  
  
  METHODS
  Twenty patients with classical PKU, aged 18-34 years and under dietary control, were enrolled in the study. The control group comprised of 20 healthy subjects matched for age and sex. Interleukin 6 and 8 levels were measured by enzyme-linked immunosorbent assay (ELISA) kits in all study participants.
  
  
  RESULTS
  IL-6 concentrations in the study group ranged from 0.74 pg/ml to 1.34 pg/ml. No significant differences were found between IL-6 concentration between the study group and the control group (p = 0.989). IL-8 concentrations ranged from 17.56 pg/ml to 20.87 pg/ml. The obtained results of IL-8 levels did not differ significantly between the study group and control group (p = 0.192). No significant correlation was observed between Phe blood levels and IL-6 or IL-8 concentrations in the study group (ρ respectively: -0.225, 0.177). In a multivariate analysis, neither IL-6 nor IL-8 concentrations were correlated with sex, age, BMI and Phe levels.
  
  
  CONCLUSIONS
  Phenylketonuria is not a risk factor for changes of inflammation status as assessed by IL-6 and IL-8 concentrations.
authors.affiliation: Voivodeship Hospital, Wrocław, Poland.
authors.forename: Renata
authors.initials: R
authors.lastname: Mozrzymas
authors.affiliation: Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Duś-Żuchowska
authors.affiliation: Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poland.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Kałużny
authors.affiliation: Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poland.
authors.forename: Ewa
authors.initials: E
authors.lastname: Wenska-Chyży
authors.affiliation: Department of Pediatric Gastroenterology and Metabolic Diseases, Poznan University of Medical Sciences, Poland.
authors.forename: Jarosław
authors.initials: J
authors.lastname: Walkowiak
mesh_terms: D000293:Adolescent; D000328:Adult; D015992:Body Mass Index; D001835:Body Weight; D016022:Case-Control Studies; D004797:Enzyme-Linked Immunosorbent Assay; D005260:Female; D006801:Humans; D007249:Inflammation / Q000097:blood*; D015850:Interleukin-6 / Q000097:blood*; D016209:Interleukin-8 / Q000097:blood*; D016014:Linear Models; D008297:Male; D010649:Phenylalanine / Q000097:blood; D010661:Phenylketonurias / Q000097:blood*; D012307:Risk Factors; D055815:Young Adult
chemical_list: C508606:CXCL8 protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D016209:Interleukin-8; D010649:Phenylalanine
keywords: inflammation; interleukin 6; interleukin 8; phenylketonuria