title: Inhibition of invasion by N-trans-feruloyloctopamine via AKT, p38MAPK and EMT related signals in hepatocellular carcinoma cells.
abstract: N-trans-feruloyloctopamine (FO) isolated from Garlic skin was identified as the primary antioxidant constituents, however, the effect of which on HCC invasion is still unclear. Herein, the FO was synthesized and its antitumor activities were evaluated in HCC cell lines. Cellular functional analyses have revealed that the reformed FO owns strong abilities of inhibiting cell proliferation and invasion in HCC cells. Molecular data have further showed that FO could significantly decrease the phosphorylation levels of Akt and p38 MAPK. In addition, the expression of Slug was inhibited and the level of E-cadherin increased. Molecular docking analysis indicates that the H-bond and hydrophobic interactions were critical for FO and E-cadherin binding, but FO did not seem to act directly on phosphorylated Akt and p38 MAPK. We have thus concluded that reformed FO inhibits cell invasion might be directly through EMT related signals (E-cadherin) and indirectly through PI3K/Akt, p38 MAPK signaling pathways. FO might be a promising drug in HCC treatment and prognosis.
authors.affiliation: The Second Department of General Surgery, The First Hospital of Lanzhou University, Key Laboratory of Biotherapy and Regenerative Medicine, Gansu Province, The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu, China.
authors.forename: Zhong-Tian
authors.initials: ZT
authors.lastname: Bai
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730020, China.
authors.forename: Zheng-Rong
authors.initials: ZR
authors.lastname: Wu
authors.affiliation: Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Li-Li
authors.initials: LL
authors.lastname: Xi
authors.affiliation: The Second Department of General Surgery, The First Hospital of Lanzhou University, Key Laboratory of Biotherapy and Regenerative Medicine, Gansu Province, The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu, China. Electronic address: lix@lzu.edu.cn.
authors.forename: Xun
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Pharmacy, Lanzhou University, Lanzhou 730020, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Chen
authors.affiliation: The Second Department of General Surgery, The First Hospital of Lanzhou University, Key Laboratory of Biotherapy and Regenerative Medicine, Gansu Province, The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu, China.
authors.forename: Fu-Qiang
authors.initials: FQ
authors.lastname: Wang
authors.affiliation: The Second Department of General Surgery, The First Hospital of Lanzhou University, Key Laboratory of Biotherapy and Regenerative Medicine, Gansu Province, The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu, China.
authors.forename: Wen-Bo
authors.initials: WB
authors.lastname: Meng
authors.affiliation: The Second Department of General Surgery, The First Hospital of Lanzhou University, Key Laboratory of Biotherapy and Regenerative Medicine, Gansu Province, The First Clinical Medical College of Lanzhou University, Lanzhou 730000, Gansu, China.
authors.forename: Wen-Ce
authors.initials: WC
authors.lastname: Zhou
authors.affiliation: Department of Pharmacy, The First Hospital of Lanzhou University, Lanzhou 730000, China.
authors.forename: Xin-An
authors.initials: XA
authors.lastname: Wu
authors.affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
authors.forename: Xiao-Jun
authors.initials: XJ
authors.lastname: Yao
authors.affiliation: Gansu Provincial Hospital, Lanzhou 730000, China.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D045744:Cell Line, Tumor; D003373:Coumaric Acids / Q000494:pharmacology*; D058750:Epithelial-Mesenchymal Transition / Q000187:drug effects*; D006801:Humans; D008113:Liver Neoplasms / Q000201:enzymology / Q000378:metabolism / Q000473:pathology*; D009361:Neoplasm Invasiveness / Q000517:prevention & control*; D009655:Octopamine / Q000031:analogs & derivatives* / Q000494:pharmacology; D051057:Proto-Oncogene Proteins c-akt / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D048051:p38 Mitogen-Activated Protein Kinases / Q000378:metabolism*
chemical_list: D003373:Coumaric Acids; C498173:N-feruloyloctopamine; D009655:Octopamine; D051057:Proto-Oncogene Proteins c-akt; D048051:p38 Mitogen-Activated Protein Kinases
keywords: AKT; E-cadherin; Epithelial to mesenchymal transition (EMT); Hepatocellular carcinoma; MAPK; N-trans-feruloyloctopamine (FO)