title: Adipose-specific ablation ofNrf2transiently delayed high-fat diet-induced obesity by altering glucose, lipid and energy metabolism of male mice.
abstract: Nuclear factor E2-related factor 2 (NRF2) is a well-known master controller of the cellular adaptive antioxidant and detoxification response. Recent studies demonstrated altered glucose, lipid and energy metabolism in mice with a globalNrf2knockout. In the present study, we aim to determine the effects of an adipose-specific ablation ofNrf2(ASAN) on diet-induced obesity (DIO) in male mice. The 6-week-old adipose-specificNrf2knockout (NK) and itsNrf2control (NC) mice were fed with either control diet (CD) or high-fat diet (HFD) for 14 weeks. NK mice exhibited transiently delayed body weight (BW) growth from week 5 to week 11 of HFD feeding, higher daily physical activity levels and preferential use of fat over carbohydrates as a source of energy at week 8 of the CD-feeding period. After 14 weeks of feeding, NK mice showed comparable results with NC mice with respect to the overall BW and body fat content, but exhibited reduced blood glucose, reduced number but increased size of adipocytes, accompanied with elevated expression of many genes and proteins in the visceral fat related to glucose, lipid and energy metabolism (e.g.Fgf21,Pgc1a). These results indicated that NRF2 is an important mediator for glucose, lipid and energy metabolism in adipose tissue, and ASAN could have beneficial effect for prevention of DIO during the early development of mice.
authors.affiliation: Institute on Aging, Department of Geriatrics, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology1095 Jiefang Road, Wuhan 430030, Hubei, China; Pennington Biomedical Research Center/LSU System6400 Perkins Road, Baton Rouge, LA 70808, USA.
authors.forename: Le
authors.initials: L
authors.lastname: Zhang
authors.affiliation: Pennington Biomedical Research Center/LSU System 6400 Perkins Road, Baton Rouge, LA 70808, USA.
authors.forename: Kalavathi
authors.initials: K
authors.lastname: Dasuri
authors.affiliation: Pennington Biomedical Research Center/LSU System 6400 Perkins Road, Baton Rouge, LA 70808, USA.
authors.forename: Sun-Ok
authors.initials: SO
authors.lastname: Fernandez-Kim
authors.affiliation: Pennington Biomedical Research Center/LSU System 6400 Perkins Road, Baton Rouge, LA 70808, USA.
authors.forename: Annadora J
authors.initials: AJ
authors.lastname: Bruce-Keller
authors.affiliation: Pennington Biomedical Research Center/LSU System 6400 Perkins Road, Baton Rouge, LA 70808, USA.
authors.forename: Jeffrey N
authors.initials: JN
authors.lastname: Keller
keywords: Adipose tissue; adipose-specific ablation of Nrf2; diet-induced obesity; glucose and lipid and energy metabolism; nuclear factor E2-related factor 2 (NRF2)