title: Nature of fly ash amendments differently influences oxidative stress alleviation in four forest tree species and metal trace element phytostabilization in aged contaminated soil: A long-term field experiment.
abstract: Aided phytostabilization using coal fly ashes (CFAs) is an interesting technique to clean-up polluted soils and valorizing industrial wastes. In this context, our work aims to study the effect of two CFAs: silico-aluminous (CFA1) and sulfo-calcic (CFA2) ones, 10 years after their addition, on the phytostabilization of a highly Cd (cadmium), Pb (lead) and Zn (zinc) contaminated agricultural soil, with four forest tree species: Robinia pseudoacacia, Alnus glutinosa, Acer pseudoplatanus and Salix alba. To assess the effect of CFAs on trees, leaf fatty acid composition, malondialdehyde (MDA), oxidized and reduced glutathione contents ratio (GSSG: GSH), 8-hydroxy-2'-deoxyguanosine (8-OHdG), Peroxidase (PO) and Superoxide dismutase (SOD) activities were examined. Our results showed that CFA amendments decreased the CaCl2-extractable fraction of Cd and Zn from the soil. However, no significant effect was observed on metal trace element (MTE) concentrations in leaves. Fatty acid percentages were only affected by the addition of sulfo-calcic CFA. The most affected species were A. glutinosa and R. pseudoacacia in which C16:0, C18:0 and C18:2 percentages increased significantly whereas the C18:3 decreased. The addition of sulfo-calcic CFA induced the antioxidant systems response in tree leaves. An increase of SOD and POD activities in leaves of trees planted on the CFA2-amended plot was recorded. Conversely, silico-aluminous CFA generated a reduction of lipid and DNA oxidation associated with the absence or low induction of anti-oxidative processes. Our study evidenced oxidative stress alleviation in tree leaves due to CFA amendments. MTE mobility in contaminated soil and their accumulation in leaves differed with the nature of CFA amendments and the selected tree species.
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, 50, rue Ferdinand Buisson, F-62228 Calais cedex, France; Université de Carthage, Laboratoire des Sciences Horticoles LR13AGR01, Institut National Agronomique de Tunisie, 43 Ave Charles Nicolle, 1082 Tunis, Mahrajène, Tunisia.
authors.forename: Sonia
authors.initials: S
authors.lastname: Labidi
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, 50, rue Ferdinand Buisson, F-62228 Calais cedex, France; UniLaSalle, Beauvais, UP-HydrISE2012.10.102, SFR Condorcet FR CNRS 3417, 19 rue Pierre Waguet, Beauvais Cedex, France.
authors.forename: Stéphane
authors.initials: S
authors.lastname: Firmin
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, 50, rue Ferdinand Buisson, F-62228 Calais cedex, France.
authors.forename: Anthony
authors.initials: A
authors.lastname: Verdin
authors.affiliation: ISA Lille, Laboratoire Génie Civil et géo Environnement (LGCgE), 48 boulevard Vauban, 59046 Lille Cedex, France.
authors.forename: Géraldine
authors.initials: G
authors.lastname: Bidar
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, 50, rue Ferdinand Buisson, F-62228 Calais cedex, France.
authors.forename: Frédéric
authors.initials: F
authors.lastname: Laruelle
authors.affiliation: ISA Lille, Laboratoire Génie Civil et géo Environnement (LGCgE), 48 boulevard Vauban, 59046 Lille Cedex, France.
authors.forename: Francis
authors.initials: F
authors.lastname: Douay
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, 50, rue Ferdinand Buisson, F-62228 Calais cedex, France.
authors.forename: Pirouz
authors.initials: P
authors.lastname: Shirali
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, 50, rue Ferdinand Buisson, F-62228 Calais cedex, France.
authors.forename: Joël
authors.initials: J
authors.lastname: Fontaine
authors.affiliation: Unité de Chimie Environnementale et Interactions sur le Vivant (UCEIV), Université du Littoral Côte d'Opale, SFR Condorcet FR CNRS 3417, 50, rue Ferdinand Buisson, F-62228 Calais cedex, France. Electronic address: lounes@univ-littoral.fr.
authors.forename: Anissa
authors.initials: A
authors.lastname: Lounès-Hadj Sahraoui
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D031002:Acer; D029661:Alnus; D001673:Biodegradation, Environmental; D002104:Cadmium / Q000032:analysis; D060729:Coal Ash; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D005227:Fatty Acids / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D007854:Lead / Q000032:analysis; D008315:Malondialdehyde / Q000378:metabolism; D018384:Oxidative Stress; D009195:Peroxidase / Q000378:metabolism; D018515:Plant Leaves / Q000737:chemistry / Q000201:enzymology / Q000378:metabolism*; D031301:Robinia; D032108:Salix; D012987:Soil / Q000737:chemistry*; D012989:Soil Pollutants / Q000032:analysis*; D013482:Superoxide Dismutase / Q000378:metabolism; D013997:Time Factors; D014131:Trace Elements / Q000032:analysis; D014197:Trees / Q000378:metabolism*; D015032:Zinc / Q000032:analysis
chemical_list: D060729:Coal Ash; D005227:Fatty Acids; D012987:Soil; D012989:Soil Pollutants; D014131:Trace Elements; D002104:Cadmium; D007854:Lead; D008315:Malondialdehyde; D000080242:8-Hydroxy-2'-Deoxyguanosine; D009195:Peroxidase; D013482:Superoxide Dismutase; D003849:Deoxyguanosine; D005978:Glutathione; D015032:Zinc
keywords: Coal fly ashes; Forest trees; MTE mobility; Oxidative stress; Phytostabilization