title: Enteric glial reactivity to systemic LPS administration: Changes in GFAP and S100B protein.
abstract: Lipopolysaccharide (LPS) is used to induce inflammation and promotes nervous system activation. Different regions of the brain present heterogeneous glial responses; thus, in order to verify whether systemic LPS-induced inflammation affects the enteric glia differently across the intestinal segments, we evaluated the expressions of two glial activity markers, GFAP and S100B protein, in different intestine segments, at 1h, 24h and 7days after acute systemic LPS administration (0.25 or 2.5mgkg-1) in rats. Histological inflammatory analysis indicated that the cecum was most affected when compared to the duodenum and proximal colon at the highest doses of LPS. LPS induced an increased S100B content after 24h in all three regions, which decreased at 7days after the highest dose in all regions. Moreover, at 24h, this dose of LPS increased ex-vivo S100B secretion only in the cecum. The highest dose of LPS also increased GFAP in all regions at 24h, but earlier in the cecum, where LPS-induced enteric S100B and GFAP alterations were dependent on dose, time and intestine region. No associated changes in serum S100B were observed. Our results indicate heterogeneous enteric glial responses to inflammatory insult, as observed in distinct brain areas.
authors.affiliation: Laboratory of Comparative Neurobiology, Department of Physiology, Institute of Basic Health Sciences (ICBS), Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Graduate Program in Neuroscience, ICBS, UFRGS, Brazil.
authors.forename: Raphaela
authors.initials: R
authors.lastname: da Cunha Franceschi
authors.affiliation: Laboratory of Calcium-Binding Proteins, Department of Biochemistry, ICBS, UFRGS, Brazil.
authors.forename: Patrícia
authors.initials: P
authors.lastname: Nardin
authors.affiliation: Laboratory of Comparative Neurobiology, Department of Physiology, Institute of Basic Health Sciences (ICBS), Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Graduate Program in Neuroscience, ICBS, UFRGS, Brazil.
authors.forename: Clivia Valle
authors.initials: CV
authors.lastname: Machado
authors.affiliation: Laboratory of Calcium-Binding Proteins, Department of Biochemistry, ICBS, UFRGS, Brazil.
authors.forename: Lucas Silva
authors.initials: LS
authors.lastname: Tortorelli
authors.affiliation: Health Science Center, University of Cruz Alta, Brazil.
authors.forename: Malcon Andrei
authors.initials: MA
authors.lastname: Martinez-Pereira
authors.affiliation: Laboratory of Calcium-Binding Proteins, Department of Biochemistry, ICBS, UFRGS, Brazil.
authors.forename: Caroline
authors.initials: C
authors.lastname: Zanotto
authors.affiliation: Graduate Program in Neuroscience, ICBS, UFRGS, Brazil; Laboratory of Calcium-Binding Proteins, Department of Biochemistry, ICBS, UFRGS, Brazil. Electronic address: casg@ufrgs.br.
authors.forename: Carlos-Alberto
authors.initials: CA
authors.lastname: Gonçalves
authors.affiliation: Laboratory of Comparative Neurobiology, Department of Physiology, Institute of Basic Health Sciences (ICBS), Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil; Graduate Program in Neuroscience, ICBS, UFRGS, Brazil.
authors.forename: Denise Maria
authors.initials: DM
authors.lastname: Zancan
mesh_terms: D000818:Animals; D002432:Cecum / Q000378:metabolism*; D003106:Colon / Q000378:metabolism*; D004305:Dose-Response Relationship, Drug; D004386:Duodenum / Q000378:metabolism*; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism*; D007249:Inflammation / Q000139:chemically induced / Q000378:metabolism*; D008070:Lipopolysaccharides / Q000008:administration & dosage* / Q000276:immunology / Q000494:pharmacology; D008297:Male; D009457:Neuroglia / Q000378:metabolism*; D051381:Rats; D064568:S100 Calcium Binding Protein beta Subunit / Q000097:blood / Q000378:metabolism*
chemical_list: D005904:Glial Fibrillary Acidic Protein; D008070:Lipopolysaccharides; D064568:S100 Calcium Binding Protein beta Subunit; C579158:S100b protein, rat
keywords: Enteric glia cell; GFAP; Inflammation; LPS; S100B