title: Design and Construction of a Cloning Vector Containing thehspXGene ofMycobacterium tuberculosis.
abstract: Tuberculosis (TB) is a major cause of death worldwide. Finding an effective vaccine against TB is the best way to control it. Several vaccines against this disease have been developed but none are completely protective. The aim of this study was to design and construct a cloning vector containing theMycobacterium tuberculosis (M. tuberculosis) heat shock protein X (hspX).
  
  
  
  First, anhspXfragment was amplified by PCR and cloned into plasmid pcDNA3.1(+) and recombinant vector was confirmed.
  
  
  
  A 435 bphspXfragment was isolated. The fragment was 100% homologous withhspXof M. tuberculosis strain H37Rv in GenBank.
  
  
  
  In this study, the cloning vector pcDNA3.1(+), containing a 435-bphspXfragment ofM. tuberculosis, was constructed. This could be used as a DNA vaccine to induce immune responses in animal models in future studies.
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Atieh
authors.initials: A
authors.lastname: Yaghoubi
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ehsan
authors.initials: E
authors.lastname: Aryan
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Hosna
authors.initials: H
authors.lastname: Zare
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Shadi
authors.initials: Sh
authors.lastname: Alami
authors.affiliation: Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran.
authors.forename: Roghayeh
authors.initials: R
authors.lastname: Teimourpour
authors.affiliation: Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Meshkat
keywords: Cloning; DNA vaccine; Mycobacterium tuberculosis; hspX