title: Tibeticola sediminis gen. nov., sp. nov., a thermophilic bacterium isolated from a hot spring.
abstract: Two closely related thermophilic bacterial strains, designated YIM 73013T and YIM 73008, were isolated from a sediment sample collected from a hot spring in Tibet, western Tibet province, China. The taxonomic positions of the two isolates were investigated using a polyphasic approach. The novel isolates were Gram-stain-negative, aerobic, short-rod-shaped and motile by means of a polar flagellum. They were oxidase- and catalase-positive and were able to grow at 30-55 °C (optimum, 37-45 °C), at pH 6.0-8.0 (optimum, pH 7.0) and with NaCl tolerance up to 1 % (w/v). Phylogenetic analyses based on 16S rRNA gene sequences showed that strains YIM 73013T and YIM 73008 formed a distinct lineage with respect to closely related genera in the family Comamonadaceae and shared highest 16S rRNA gene sequences similarities with Acidovorax caeni R-24608T (96.3 and 96.4 %, respectively). The respiratory quinone was ubiquinone-8 (Q-8) and the major cellular fatty acids observed were C17 : 1ω6c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The genomic DNA G+C contents of strains YIM 73013T and YIM 73008 were 68.7 and 68.3 mol%, respectively. Based on the morphological, phylogenetic and chemotaxonomic results, the two isolates represent a novel species in a new genus, for which the name Tibeticola sediminis gen. nov., sp. nov. is proposed. The type strain of Tibeticola sediminis is YIM 73013T (=DSM 101684T=KCTC 42873T).
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.
authors.forename: Inam Ullah
authors.initials: IU
authors.lastname: Khan
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.
authors.forename: Firasat
authors.initials: F
authors.lastname: Hussain
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.
authors.forename: Neeli
authors.initials: N
authors.lastname: Habib
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Wen-Dong
authors.initials: WD
authors.lastname: Xian
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Jiang
authors.affiliation: Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Centre (NARC), Islamabad 45500, Pakistan.|Department of Microbiology, Quaid-i-Azam University, Islamabad 45320, Pakistan.
authors.forename: Arshia
authors.initials: A
authors.lastname: Amin
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.
authors.forename: Chang-Guo
authors.initials: CG
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: En-Min
authors.initials: EM
authors.lastname: Zhou
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.
authors.forename: Xiao-Yang
authors.initials: XY
authors.lastname: Zhi
authors.affiliation: Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, PR China.|State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China.
authors.forename: Wen-Jun
authors.initials: WJ
authors.lastname: Li
mesh_terms: D015373:Bacterial Typing Techniques; D001482:Base Composition; D042621:Comamonadaceae / Q000145:classification* / Q000235:genetics / Q000302:isolation & purification; D004269:DNA, Bacterial / Q000235:genetics; D005227:Fatty Acids / Q000737:chemistry; D045482:Hot Springs / Q000382:microbiology*; D010802:Phylogeny; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D017422:Sequence Analysis, DNA; D018609:Tibet; D014451:Ubiquinone / Q000737:chemistry
chemical_list: D004269:DNA, Bacterial; D005227:Fatty Acids; D012336:RNA, Ribosomal, 16S; D014451:Ubiquinone; C030778:ubiquinone 8