title: Assembling of multifunctional latex-based hybrid nanocarriers from Calotropis gigantea for sustained (doxorubicin) DOX releases.
abstract: Natural rubber Latex (Lax) is a colloidal dispersion of polymer particles in liquid and shows good biodegradable, biocompatibility, and non-toxicity. Natural polymers are the most important materials used in food packaging, micro/nano-drug delivery, tissue engineering, agriculture, and coating. In the present study, natural compounds extracted from plant Lax were designed to function as drug carriers using various surfactants via emulation and solvent evaporation method. Calotropis gigantea belongs to the family Apocynaceae and has received considerable attention in modern medicine, ayurvedeic, siddha, and traditional medicine. Since, we were isolated biodegradable, non-toxic, and biocompatible materials as latex from Calotropis gigantea plant. The Lax was separated as per their solubility nature and it was designed as a carrier using surfactant namely; Sorbitanmonolaurate (Span-20), sodium lauryl sulfate (SLS), and cetyltrimethylammonium bromide (CTAB). The isolated compounds from Lax of Calotropis gigantea were analyzed using high-performance liquid chromatography. To confirm the encapsulation efficiency and in vitro drug release of the carriers, doxorubicin (DOX) was used as a model natural drug. The hybrid nanocarriers were successfully synthesized through simple solvent evaporation using three surfactants, and the morphology was characterized by SEM and TEM technique. The functionality and crystalline nature of the nanocarriers were confirmed using FTIR and XRD, respectively. Within 90min, the maximum amount of DOX was encapsulated in the carriers, and prolonged cumulative drug release by the nanocarriers was observed. The formulated natural carriers were found to have potentially effective cytotoxic effects on lung cancer cells.
authors.affiliation: Biomaterials in Medicinal Chemistry Laboratory, Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India.
authors.forename: Periyakaruppan
authors.initials: P
authors.lastname: Pradeepkumar
authors.affiliation: Biomaterials in Medicinal Chemistry Laboratory, Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India.
authors.forename: Dharman
authors.initials: D
authors.lastname: Govindaraj
authors.affiliation: National Centre for Nanoscience and Nanotechnology, University of Madras, Guindy campus, Chennai, 25, India.
authors.forename: Murugaraj
authors.initials: M
authors.lastname: Jeyaraj
authors.affiliation: Student training Unit, Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
authors.forename: Murugan A
authors.initials: MA
authors.lastname: Munusamy
authors.affiliation: Biomaterials in Medicinal Chemistry Laboratory, Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai, 625021, Tamil Nadu, India. Electronic address: rajanm153@gmail.com.
authors.forename: Mariappan
authors.initials: M
authors.lastname: Rajan
mesh_terms: D000072283:A549 Cells; D031107:Calotropis / Q000737:chemistry*; D045744:Cell Line, Tumor; D000077286:Cetrimonium; D002593:Cetrimonium Compounds / Q000737:chemistry; D002626:Chemistry, Pharmaceutical / Q000379:methods; D003692:Delayed-Action Preparations / Q000737:chemistry* / Q000494:pharmacology; D004317:Doxorubicin / Q000737:chemistry* / Q000494:pharmacology; D004337:Drug Carriers / Q000737:chemistry*; D006601:Hexoses / Q000737:chemistry; D006801:Humans; D007840:Latex / Q000737:chemistry*; D008175:Lung Neoplasms / Q000188:drug therapy; D053758:Nanoparticles / Q000737:chemistry*; D010936:Plant Extracts / Q000737:chemistry; D012967:Sodium Dodecyl Sulfate / Q000737:chemistry; D012995:Solubility
chemical_list: D002593:Cetrimonium Compounds; D003692:Delayed-Action Preparations; D004337:Drug Carriers; D006601:Hexoses; D007840:Latex; D010936:Plant Extracts; D012967:Sodium Dodecyl Sulfate; C014822:sorbitan monolaurate; D004317:Doxorubicin; D000077286:Cetrimonium
keywords: Biodegradable; Calotropis gigantea; Cancer; Drug delivery; Emulation method; Surfactant