title: Rapid Molecular Approach for Simultaneous Detection ofSalmonellaspp.,Shigellaspp., andVibrio cholera.
abstract: OBJECTIVE
  Gastrointestinal tract infection is still one of the serious public health problems in many geographic areas and is endemic in most countries including Iran. Early detection of the gastrointestinal tract pathogens can be extremely important. The aim of the current study was to apply a shortened time-multiplex polymerase chain reaction (PCR) for rapid and simultaneous detection ofSalmonellaspp.,Shigellaspp., andVibrio cholera.
  
  
  METHODS
  The standard and clinical strains ofSalmonellaspp.,Shigellaspp., andV. choleraewere used in the assay. Multiplex PCR was performed and optimized based on amplification ofinvA, putative integrase, andompWgenes for detectingSalmonellaspp.,Shigellaspp., andV. cholerae, respectively. The specificity of the assay was evaluated by testing 12 different bacterial species.
  
  
  RESULTS
  OnlySalmonellaspp.,Shigellaspp., andV. choleraestrains had positive results when subjected to the assay using multiplex PCR. The assay showed a high sensitivity, and no amplification products were observed in multiplex PCR with any of the other microorganisms.
  
  
  CONCLUSIONS
  Our study indicated that theinvA, putative integrase, andompW-based multiplex PCR assay appears to be an efficient method for rapid and simultaneous detection ofSalmonellaspp.,Shigellaspp., andV. cholerae.
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Reza
authors.initials: R
authors.lastname: Ranjbar
authors.affiliation: Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Naghoni
authors.affiliation: Department of Microbiology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran.
authors.forename: Davoud
authors.initials: D
authors.lastname: Afshar
authors.affiliation: Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
authors.forename: Farhad
authors.initials: F
authors.lastname: Nikkhahi
authors.affiliation: Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Lorestan University of Medical Sciences, Khorramabad, Iran.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Mohammadi
keywords: Salmonella spp.; Shigella spp.; Vibrio cholerae; multiplex PCR