title: Short-term high-fat diet compromises myocardial function: a radial strain rate imaging study.
abstract: OBJECTIVE
  Long-term high-fat diet (HFD) induces both cardiac remodelling and myocardial dysfunction in murine models. The aim was to assess the time course and mechanisms of metabolic and cardiac modifications induced by short-term HFD in wild-type (WT) mice.
  
  
  RESULTS
  Thirty-three WT mice were subjected to HFD (60% fat, n = 16) and chow diet (CD, 13% fat, n = 17). Metabolic and echocardiographic data were collected at baseline and every 5 weeks for 20 weeks. Invasive haemodynamic data and myocardial samples were collected at 5 and 20 weeks. Echocardiographic data included left ventricular (LV) diameters and thickness, and systolic function using radial strain rate (SR). Histological assessment of cardiomyocyte and adipocyte sizes, interstitial fibrosis, and apoptosis index were performed. During follow-up, body weight, and glycaemia levels were higher in HFD than in CD mice, in association with an early adipose tissue remodelling. Despite no difference between both groups in blood pressure and LV mass at 5 weeks, an early LV dysfunction was observed in HFD mice as assessed by radial SR (21 ± 0.8 vs. 27 ± 0.8 unit/s, P < 0.001) and haemodynamic assessment. During follow-up, both groups demonstrated a progressive systolic and diastolic LV dysfunction and remodelling including dilatation and hypertrophy, which were more severe in HFD mice. Compared with CD mice, the early LV impairment in HFD mice was coupled with a higher cardiomyocyte apoptosis level (0.95 vs. 0.02%, P < 0.05) associated with an interstitial fibrosis process (2.3 vs. 0.2%, P < 0.05), which worsen during follow-up.
  
  
  CONCLUSIONS
  The HFD promoted early metabolic and cardiac dysfunctions, and adipose and myocardial tissues remodelling.
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.|AP-HP, Department of Cardiology, Henri Mondor Hospital, DHU-ATVB, Créteil, France.
authors.forename: Julien
authors.initials: J
authors.lastname: Ternacle
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.
authors.forename: Feng
authors.initials: F
authors.lastname: Wan
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.
authors.forename: Daigo
authors.initials: D
authors.lastname: Sawaki
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.|AP-HP Vaccine Research Institute (VRI), Créteil F-94010, France.
authors.forename: Mathieu
authors.initials: M
authors.lastname: Surenaud
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.
authors.forename: Maria
authors.initials: M
authors.lastname: Pini
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.
authors.forename: Raquel
authors.initials: R
authors.lastname: Mercedes
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.|AP-HP, Department of Physiology, Henri Mondor Hospital, DHU-ATVB, Créteil, France.
authors.forename: Laura
authors.initials: L
authors.lastname: Ernande
authors.affiliation: AP-HP, Public Health Department, Henri Mondor Hospital, DHU-ATVB, Créteil, France.
authors.forename: Etienne
authors.initials: E
authors.lastname: Audureau
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.|AP-HP, Department of Cardiology, Henri Mondor Hospital, DHU-ATVB, Créteil, France.
authors.forename: Jean-Luc
authors.initials: JL
authors.lastname: Dubois-Rande
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.|AP-HP, Department of Physiology, Henri Mondor Hospital, DHU-ATVB, Créteil, France.
authors.forename: Serge
authors.initials: S
authors.lastname: Adnot
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.|AP-HP Vaccine Research Institute (VRI), Créteil F-94010, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Hue
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.
authors.forename: Gabor
authors.initials: G
authors.lastname: Czibik
authors.affiliation: INSERM U955, Université Paris-Est Creteil (UPEC), 51 Av de Lattre de Tassigny, 94100 Créteil, France.|AP-HP, Department of Cardiology, Henri Mondor Hospital, DHU-ATVB, Créteil, France.
authors.forename: Genevieve
authors.initials: G
authors.lastname: Derumeaux
mesh_terms: D000818:Animals; D017209:Apoptosis; D015415:Biomarkers / Q000378:metabolism; D009202:Cardiomyopathies / Q000000981:diagnostic imaging* / Q000209:etiology*; D059305:Diet, High-Fat; D004452:Echocardiography; D005951:Glucose Tolerance Test; D006801:Humans; D020287:In Situ Nick-End Labeling; D007328:Insulin / Q000378:metabolism; D008297:Male; D008315:Malondialdehyde / Q000378:metabolism; D051379:Mice; D008810:Mice, Inbred C57BL; D020257:Ventricular Remodeling
chemical_list: D015415:Biomarkers; D007328:Insulin; D008315:Malondialdehyde
keywords: cardiomyopathy; high-fat diet; obesity; strain rate imaging