title: 3-hydroxi-anthranilic acid is early expressed in stroke.
abstract: Using an immunohistochemical technique, we have studied the distribution of 3-OH-anthranilic acid (3-HAA) in the rat brain. Our study was carried out in control animals and in rats in which a stroke model (single transient middle cerebral artery occlusion) was performed. A monoclonal antibody directed against 3-HAA was also developed. 3-HAA was exclusively observed in the infarcted regions (ipsilateral striatum/cerebral cortex), 2, 5 and 21 days after the induction of stroke. In control rats and in the contralateral side of the stroke animals, no immunoreactivity for 3-HAA was visualized. Under pathological conditions (from early phases of stroke), we reported for the first time the presence of 3-HAA in the mammalian brain. By double immunohistochemistry, the coexistence of 3-HAA and GFAP was observed in astrocytes. The distribution of 3-HAA matched perfectly with the infarcted regions. Our findings suggest that, in stroke, 3-HAA could be involved in the tissue damage observed in the infarcted regions, since it is well known that 3-HAA exerts cytotoxic effects.
authors.affiliation: Gemacbio - Institute for the Development of Research in Human Pathology and Therapeutic (IDRPHT) - University of Salamanca. amangas@gemacbio.com.
authors.forename: A
authors.initials: A
authors.lastname: Mangas
authors.forename: J
authors.initials: J
authors.lastname: Yajeya
authors.forename: N
authors.initials: N
authors.lastname: González
authors.forename: I
authors.initials: I
authors.lastname: Ruiz
authors.forename: M
authors.initials: M
authors.lastname: Geffard
authors.forename: R
authors.initials: R
authors.lastname: Coveñas
mesh_terms: D015095:3-Hydroxyanthranilic Acid / Q000378:metabolism*; D000818:Animals; D001921:Brain / Q000378:metabolism* / Q000473:pathology; D001923:Brain Chemistry; D008297:Male; D051381:Rats; D017208:Rats, Wistar; D020521:Stroke / Q000378:metabolism* / Q000473:pathology
chemical_list: D015095:3-Hydroxyanthranilic Acid