title: Mitigation of Membrane Biofouling in MBR Using a Cellulolytic Bacterium,Undibacteriumsp. DM-1, Isolated from Activated Sludge.
abstract: Biofilm formation on the membrane surface results in the loss of permeability in membrane bioreactors (MBRs) for wastewater treatment. Studies have revealed that cellulose is not only produced by a number of bacterial species but also plays a key role during formation of their biofilm. Hence, in this study, cellulase was introduced to a MBR as a cellulose-induced biofilm control strategy. For practical application of cellulase to MBR, a cellulolytic (i.e., cellulase-producing) bacterium,Undibacteriumsp. DM-1, was isolated from a lab-scale MBR for wastewater treatment. Prior to its application to MBR, it was confirmed that the cell-free supernatant of DM-1 was capable of inhibiting biofilm formation and of detaching the mature biofilm of activated sludge and cellulose-producing bacteria. This suggested that cellulase could be an effective anti-biofouling agent for MBRs used in wastewater treatment.Undibacteriumsp. DM-1-entrapping beads (i.e., cellulolytic-beads) were applied to a continuous MBR to mitigate membrane biofouling 2.2-fold, compared with an MBR with vacant-beads as a control. Subsequent analysis of the cellulose content in the biofilm formed on the membrane surface revealed that this mitigation was associated with an approximately 30% reduction in cellulose by cellulolytic-beads in MBR.
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Chang Hyun
authors.initials: CH
authors.lastname: Nahm
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Seonki
authors.initials: S
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Sang Hyun
authors.initials: SH
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Kibaek
authors.initials: K
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Jaewoo
authors.initials: J
authors.lastname: Lee
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Hyeokpil
authors.initials: H
authors.lastname: Kwon
authors.affiliation: Department of Environmental Engineering, Kyungpook National University, Daegu 41566, Republic of Korea.
authors.forename: Kwang-Ho
authors.initials: KH
authors.lastname: Choo
authors.affiliation: Department of Biomedicinal Science and Biotechnology, Paichai University, Daejeon 35345, Republic of Korea.
authors.forename: Jung-Kee
authors.initials: JK
authors.lastname: Lee
authors.affiliation: Pure Envitech Co., Ltd, Siheung 15116, Republic of Korea.
authors.forename: Jae Young
authors.initials: JY
authors.lastname: Jang
authors.affiliation: School of Chemical and Biological Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Chung-Hak
authors.initials: CH
authors.lastname: Lee
authors.affiliation: Department of Environmental Engineering, Yonsei University, Wonju 26439, Republic of Korea.
authors.forename: Pyung-Kyu
authors.initials: PK
authors.lastname: Park
mesh_terms: D018441:Biofilms; D057219:Biofouling; D019149:Bioreactors; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism*; D005285:Fermentation; D042802:Oxalobacteraceae / Q000302:isolation & purification / Q000502:physiology*; D012722:Sewage / Q000382:microbiology*
chemical_list: D012722:Sewage; D002482:Cellulose; D002480:Cellulase
keywords: Cellulose; Undibacterium sp. DM-1; anti-biofouling; cellulase; membrane bioreactors