title: Decreased intracellular [Ca2+] coincides with reduced expression of Dhprα1s, RyR1, and diaphragmatic dysfunction in a rat model of sepsis.
abstract: BACKGROUND
  Sepsis can cause decreased diaphragmatic contractility. Intracellular calcium as a second messenger is central to diaphragmatic contractility. However, changes in intracellular calcium concentration ([Ca2+]) and the distribution and co-localization of relevant calcium channels [dihydropyridine receptors, (DHPRα1s) and ryanodine receptors (RyR1)] remain unclear during sepsis. In this study we investigated the effect of changed intracellular [Ca2+] and expression and distribution of DHPRα1s and RyR1 on diaphragm function during sepsis.
  
  
  METHODS
  We measured diaphragm contractility and isolated diaphragm muscle cells in a rat model of sepsis. The distribution and co-localization of DHPRα1s and RyR1 were determined using immunohistochemistry and immunofluorescence, whereas intracellular [Ca2+] was measured by confocal microscopy and fluorescence spectrophotometry.
  
  
  RESULTS
  Septic rat diaphragm contractility, expression of DHPRα1s and RyR1, and intracellular [Ca2+] were significantly decreased in the rat sepsis model compared with controls.
  
  
  CONCLUSIONS
  Decreased intracellular [Ca2+] coincides with diaphragmatic contractility and decreased expression of DHPRα1s and RyR1 in sepsis. Muscle Nerve 56: 1128-1136, 2017.
authors.affiliation: Department of Respiratory and Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang, Postal Code 110004, People's Republic of China.
authors.forename: Meng-Meng
authors.initials: MM
authors.lastname: Wang
authors.affiliation: Department of Pharmaceutical Toxicology, School of Pharmaceutical Sciences, China Medical University, Shenyang, People's Republic of China.
authors.forename: Li-Ying
authors.initials: LY
authors.lastname: Hao
authors.affiliation: Department of Pharmaceutical Toxicology, School of Pharmaceutical Sciences, China Medical University, Shenyang, People's Republic of China.
authors.forename: Feng
authors.initials: F
authors.lastname: Guo
authors.affiliation: Department of Respiratory Medicine, The First Affiliated Hospital of Gannan Medical University, Ganzhou, Jiangxi Province, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhong
authors.affiliation: Department of Respiratory and Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang, Postal Code 110004, People's Republic of China.
authors.forename: Xiao-Mei
authors.initials: XM
authors.lastname: Zhong
authors.affiliation: Department of Respiratory and Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang, Postal Code 110004, People's Republic of China.
authors.forename: Jing
authors.initials: J
authors.lastname: Yuan
authors.affiliation: Department of Orthopedic Surgery, Shengjing Hospital of China Medical University, Shenyang, People's Republic of China.
authors.forename: Yi-Fei
authors.initials: YF
authors.lastname: Hao
authors.affiliation: Department of Respiratory and Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang, Postal Code 110004, People's Republic of China.
authors.forename: Shuang
authors.initials: S
authors.lastname: Zhao
authors.affiliation: Department of Pharmaceutical Toxicology, School of Pharmaceutical Sciences, China Medical University, Shenyang, People's Republic of China.
authors.forename: Xue-Fei
authors.initials: XF
authors.lastname: Sun
authors.affiliation: Department of Pharmaceutical Toxicology, School of Pharmaceutical Sciences, China Medical University, Shenyang, People's Republic of China.
authors.forename: Ming
authors.initials: M
authors.lastname: Lei
authors.affiliation: Department of Respiratory and Intensive Care Unit, Shengjing Hospital of China Medical University, Shenyang, Postal Code 110004, People's Republic of China.
authors.forename: Guang-Yu
authors.initials: GY
authors.lastname: Jiao
mesh_terms: D000818:Animals; D002118:Calcium / Q000378:metabolism*; D020746:Calcium Channels, L-Type / Q000096:biosynthesis* / Q000235:genetics; D003964:Diaphragm / Q000378:metabolism* / Q000503:physiopathology; D015870:Gene Expression; D007424:Intracellular Fluid / Q000378:metabolism*; D008297:Male; D009119:Muscle Contraction / Q000502:physiology; D009924:Organ Culture Techniques; D051381:Rats; D017207:Rats, Sprague-Dawley; D019837:Ryanodine Receptor Calcium Release Channel / Q000096:biosynthesis* / Q000235:genetics; D018805:Sepsis / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology
chemical_list: C529218:CACNA1S protein, mouse; D020746:Calcium Channels, L-Type; D019837:Ryanodine Receptor Calcium Release Channel; D002118:Calcium
keywords: DHPRα1s; RyR1; diaphragm; intracellular [Ca2+]; sepsis