title: Photo-Cross-Linkable Polymer Dots with Stable Sensitizer Loading and Amplified Singlet Oxygen Generation for Photodynamic Therapy.
abstract: Photodynamic therapy (PDT) is a promising treatment modality for clinical cancer therapy. However, the therapeutic effect of PDT is strongly dependent on the property of photosensitizer. Here, we developed photo-cross-linkable semiconductor polymer dots doped with photosensitizer Chlorin e6 (Ce6) to construct a nanoparticle platform for photodynamic therapy. Photoreactive oxetane groups were attached to the side chains of the semiconductor polymer. After photo-cross-linking reaction, the Ce6-doped Pdots formed an interpenetrated structure to prevent Ce6 leaching out from the Pdot matrix. Spectroscopic characterizations revealed an efficient energy transfer from the polymer to Ce6 molecules, resulting in amplified generation of singlet oxygen. We evaluated the cellular uptake, cytotoxicity, and photodynamic effect of the Pdots in gastric adenocarcinoma cells. In vitro photodynamic experiments indicated that the Ce6-doped Pdots (∼10 μg/mL) effectively killed the cancer cells under low dose of light irradiation (∼60 J/cm2). Furthermore, in vivo photodynamic experiments were carried out in tumor-bearing nude mice, which indicated that the Pdot photosensitizer apparently suppressed the growth of solid tumors. Our results demonstrate that the photo-cross-linkable Pdots doped with photosensitizer are promising for photodynamic cancer treatment.
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University , Changchun 130021, China.
authors.forename: Ying
authors.identifier: http://orcid.org/0000-0002-1105-9003
authors.initials: Y
authors.lastname: Tang
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , Changchun 130012, China.
authors.forename: Haobin
authors.initials: H
authors.lastname: Chen
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , Changchun 130012, China.
authors.forename: Kaiwen
authors.initials: K
authors.lastname: Chang
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , Changchun 130012, China.
authors.forename: Zhihe
authors.initials: Z
authors.lastname: Liu
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University , Changchun 130021, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Luminescence and Applications, Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences , Changchun 130033, China.
authors.forename: Songnan
authors.initials: S
authors.lastname: Qu
authors.affiliation: Department of Gastroenterology, The First Hospital of Jilin University , Changchun 130021, China.
authors.forename: Hong
authors.initials: H
authors.lastname: Xu
authors.affiliation: State Key Laboratory on Integrated Optoelectronics, College of Electronic Science and Engineering, Jilin University , Changchun 130012, China.
authors.forename: Changfeng
authors.initials: C
authors.lastname: Wu
mesh_terms: D000818:Animals; D045744:Cell Line, Tumor; D051379:Mice; D008819:Mice, Nude; D010778:Photochemotherapy; D017319:Photosensitizing Agents; D011108:Polymers; D011166:Porphyrins; D026082:Singlet Oxygen / Q000737:chemistry*
chemical_list: D017319:Photosensitizing Agents; D011108:Polymers; D011166:Porphyrins; D026082:Singlet Oxygen
keywords: energy transfer; photo-cross-linking; photodynamic therapy; photosensitizer; semiconducting polymer