title: [Effect of umbilical cord mesenchymal stem cell transplantation on immune function and prognosis of patients with decompensated hepatitis B cirrhosis].
abstract: Objective:To investigate the effect of human umbilical cord mesenchymal stem cells (hUCMSCs) on the immune function and prognosis of patients with decompensated hepatitis B cirrhosis.Methods:A total of 65 patients with decompensated hepatitis B cirrhosis were divided into observation group and control group. The patients in the observation group were given intervention (via the proper hepatic artery or the portal vein) and intravenous infusion of 4×108hUCMSCs in two doses, as well as the same basic treatment as in the control group. The patients in the control group were given conventional medical treatment. ELISA as used to measure the serum levels of interleukin-6 (IL-6), tumor necrosis factor-α (TNFα), interleukin-10 (IL-10), and transforming growth factor-β (TGFβ) in the observation group before surgery and at 1 week after surgery, as well as the serum levels of IL-6, TNFα, IL-10, and TGFβ in the control group on admission and at 1 week after admission. Flow cytometry was used to measure the percentage of lymphocyte subsets in the observation group before surgery and at 1 week after surgery, as well as that in the control group on admission and at 1 week after admission. In addition, the patients' prognosis and major complications during hospitalization were observed in both groups, and the patients were followed up for 24 weeks to record the number of deaths. The t-test was used for comparison of continuous data, and the chi-square test was used for comparison of categorical data which were expressed as percentages.Results:At 1 week after the transplantation of hUCMSCs, compared with the control group, the observation group had significant reductions in the serum levels of IL-6 and TNFα and significant increases in the serum levels of IL-10 and TGFβ (allP< 0.001), as well as significant increases in the percentages of T4 cells and Treg cells and significant reductions in the percentages of T8 cells and B cells (allP< 0.05). There were no significant differences in the changes in T3 cells and natural killer cells between the two groups (P> 0.05). Compared with the control group, the observation group had a significantly lower probability of progression to liver failure (6.45% vs 14.71%,P= 0.017).Conclusion:In the treatment of patients with decompensated hepatitis B cirrhosis, transplantation of UCMSCs can inhibit the proliferation of T cells and B cells and the differentiation of T8 cells, upregulate Treg cells, promote the secretion of immunosuppressive cytokines, and reduce the production of inflammatory cytokines. Therefore, it can alleviate liver inflammatory response and liver cell damage and reduce the probability of hepatic failure.
authors.affiliation: Clinical College of PLA of Anhui Medical University, Hefei 230001, China.
authors.forename: X Q
authors.initials: XQ
authors.lastname: Fang
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: J F
authors.initials: JF
authors.lastname: Zhang
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Song
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: Z L
authors.initials: ZL
authors.lastname: Chen
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: J
authors.initials: J
authors.lastname: Dong
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: X
authors.initials: X
authors.lastname: Chen
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: J J
authors.initials: JJ
authors.lastname: Pan
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: B
authors.initials: B
authors.lastname: Liu
authors.affiliation: The Infectious Department in the NO.105 Hospital of PLA, Hefei 230001, China.
authors.forename: C X
authors.initials: CX
authors.lastname: Chen
mesh_terms: D006801:Humans; D001402:B-Lymphocytes; D002454:Cell Differentiation; D016207:Cytokines / Q000097:blood; D005434:Flow Cytometry; D006509:Hepatitis B / Q000097:blood / Q000601:surgery; D016753:Interleukin-10 / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008103:Liver Cirrhosis / Q000097:blood / Q000601:surgery; D045164:Mesenchymal Stem Cell Transplantation; D059630:Mesenchymal Stem Cells; D011379:Prognosis; D050378:T-Lymphocytes, Regulatory; D016212:Transforming Growth Factor beta / Q000097:blood; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D014470:Umbilical Cord
chemical_list: D016207:Cytokines; C508609:IL10 protein, human; C508600:IL6 protein, human; D016753:Interleukin-10; D015850:Interleukin-6; D016212:Transforming Growth Factor beta; D014409:Tumor Necrosis Factor-alpha