title: Nitric oxide acts upstream of ethylene in cell wall phosphorus reutilization in phosphorus-deficient rice.
abstract: Nitric oxide (NO) and ethylene are both involved in cell wall phosphorus (P) reutilization in P-deficient rice; however, the crosstalk between them remains unclear. In the present study using P-deficient 'Nipponbare' (Nip), root NO accumulation significantly increased after 1 h and reached a maximum at 3 h, while ethylene production significantly increased after 3 h and reached a maximum at 6 h, indicating NO responded more quickly than ethylene. Irrespective of P status, addition of the NO donor sodium nitroprusside (SNP) significantly increased while the NO scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (c-PTIO) significantly decreased the production of ethylene, while neither the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) nor the ethylene inhibitor aminoethoxyvinylglycine (AVG) had any influence on NO accumulation, suggesting NO acted upstream of ethylene. Under P-deficient conditions, SNP and ACC alone significantly increased root soluble P content through increasing pectin content, and c-PTIO addition to the ACC treatment still showed the same tendency; however, AVG+SNP treatment had no effect, further indicating that ethylene was the downstream signal affecting pectin content. The expression of the phosphate transporter gene OsPT2 showed the same tendency as the NO-ethylene-pectin pathway. Taken together, we conclude that ethylene functions downstream of NO in cell wall P reutilization in P-deficient rice.
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, China.
authors.forename: Xiao Fang
authors.initials: XF
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, China.
authors.forename: Chun Quan
authors.initials: CQ
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, China.
authors.forename: Chao
authors.initials: C
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, China.
authors.forename: Xiao Ying
authors.initials: XY
authors.lastname: Dong
authors.affiliation: State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Science, Nanjing, China.
authors.forename: Ren Fang
authors.initials: RF
authors.lastname: Shen
mesh_terms: D002473:Cell Wall / Q000378:metabolism; D005030:Ethylenes / Q000378:metabolism*; D009569:Nitric Oxide / Q000378:metabolism*; D012275:Oryza / Q000378:metabolism*; D010758:Phosphorus / Q000378:metabolism*; D010937:Plant Growth Regulators / Q000378:metabolism*
chemical_list: D005030:Ethylenes; D010937:Plant Growth Regulators; D010758:Phosphorus; D009569:Nitric Oxide; C036216:ethylene
keywords: Cell wall; NO; Oryza sativa; ethylene; pectin; phosphorus; remobilization; translocation