title: Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells.
abstract: During tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface. Importantly, the compound CAs of PDLSCs and JBMSCs regenerated periodontal ligament-like fibers and mineralized matrix on the Ti scaffold surface, both in nude mice ectopic and minipig orthotopic transplantations. Our data revealed that an effective regenerative microenvironment, reconstructed by JBMSCs, promoted periodontium regeneration by regulating PDLSCs function on the Ti material.
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Department of Stomatology, PLA Xizang Military Region General Hospital, Lhasa, Tibet, People's Republic of China.
authors.forename: Bin
authors.initials: B
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Wenjia
authors.initials: W
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.|Department of Stomatology, PLA 301th Hospital, Beijing, People's Republic of China.
authors.forename: Yihan
authors.initials: Y
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Xicong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics Surgery, Xijing Hospital, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Zhuojing
authors.initials: Z
authors.lastname: Luo
authors.affiliation: State Key Laboratory of Military Stomatology, Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, Shaanxi, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Jin
mesh_terms: D000818:Animals; D002448:Cell Adhesion; D060833:Cellular Microenvironment; D016557:Guided Tissue Regeneration, Periodontal; D007568:Jaw / Q000502:physiology*; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D008819:Mice, Nude; D010513:Periodontal Ligament / Q000502:physiology*; D013552:Swine; D013556:Swine, Miniature