title: Expression of epithelial cell-derived cytokine genes in the duodenal and colonic mucosae of dogs with chronic enteropathy.
abstract: It remains unclear whether epithelial cell-derived cytokines, including interleukin (IL)-25, IL-33 and thymic stromal lymphopoietin (TSLP), contribute to development of canine chronic enteropathy (CE), which includes antibiotic-responsive enteropathy (ARE), food-responsive enteropathy (FRE) and inflammatory bowel disease (IBD). In the present study, we examined mRNA expression of il-25, il-33 and tslp in the duodenal and colonic mucosae of dogs with ARE, FRE and IBD. Real-time PCR analysis revealed that mRNA expression of il-33 was significantly lower in the duodenum in dogs with FRE than in healthy dogs. The results suggest that epithelial cell-derived cytokines may not be an inducer of Th2-type immunity in the gut of dogs with CE, and decreased expression of IL-33 may be involved in induction of FRE. Further studies are required to clarify roles of epithelial cell-derived cytokines, especially IL-33, in the pathogenesis of canine CE.
authors.affiliation: Cooperative Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu, Tokyo 183-8509, Japan.
authors.forename: Hironari
authors.initials: H
authors.lastname: Osada
authors.forename: Misato
authors.initials: M
authors.lastname: Ogawa
authors.forename: Ayana
authors.initials: A
authors.lastname: Hasegawa
authors.forename: Makoto
authors.initials: M
authors.lastname: Nagai
authors.forename: Junsuke
authors.initials: J
authors.lastname: Shirai
authors.forename: Kazuaki
authors.initials: K
authors.lastname: Sasaki
authors.forename: Minoru
authors.initials: M
authors.lastname: Shimoda
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Itoh
authors.forename: Hirotaka
authors.initials: H
authors.lastname: Kondo
authors.forename: Keitaro
authors.initials: K
authors.lastname: Ohmori
mesh_terms: D000818:Animals; D002908:Chronic Disease; D003106:Colon / Q000378:metabolism*; D016207:Cytokines / Q000235:genetics* / Q000378:metabolism; D004283:Dog Diseases / Q000235:genetics / Q000378:metabolism*; D004285:Dogs; D004386:Duodenum / Q000378:metabolism*; D020381:Interleukin-17 / Q000235:genetics / Q000378:metabolism; D000067596:Interleukin-33 / Q000235:genetics / Q000378:metabolism; D007410:Intestinal Diseases / Q000235:genetics / Q000378:metabolism / Q000662:veterinary*; D007413:Intestinal Mucosa / Q000378:metabolism*; D008297:Male; D012333:RNA, Messenger / Q000378:metabolism; D000094632:Thymic Stromal Lymphopoietin
chemical_list: D016207:Cytokines; D020381:Interleukin-17; D000067596:Interleukin-33; D012333:RNA, Messenger; D000094632:Thymic Stromal Lymphopoietin