title: Assessment of Vascular Dysfunction and Inflammation Induced by Angiotensin II in Mice.
abstract: Vascular inflammation in cardiovascular diseases is recognized to be linked with immune cell activation. Recruitment of immune cells into the vessel wall is an early step in angiotensin II-induced vascular dysfunction and arterial hypertension. Exploring the role of monocytes and macrophages in angiotensin II-induced hypertension and vascular inflammation in mouse models highlights the importance of these pathophysiological processes. Here we describe our routinely used protocols concerning angiotensin II-induced hypertension, assessment of blood pressure, vascular function, and immune cell infiltration.
authors.affiliation: Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, Langenbeckstrasse 1, 55131, Mainz, Germany.
authors.forename: Jeremy
authors.initials: J
authors.lastname: Lagrange
authors.affiliation: Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, Langenbeckstrasse 1, 55131, Mainz, Germany.|Center for Cardiology - cardiology I, University Medical Center Mainz, Langenbeckstrasse 1, 55131, Mainz, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Kossmann
authors.affiliation: Center for Thrombosis and Hemostasis Mainz, University Medical Center Mainz, Langenbeckstrasse 1, 55131, Mainz, Germany. wenzelp@uni-mainz.de.|Center for Cardiology - cardiology I, University Medical Center Mainz, Langenbeckstrasse 1, 55131, Mainz, Germany. wenzelp@uni-mainz.de.|German Center for Cardiovascular Research (DZHK), partner site Rhine Main, Langenbeckstrasse 1, 55131, Mainz, Germany. wenzelp@uni-mainz.de.
authors.forename: Philip
authors.initials: P
authors.lastname: Wenzel
mesh_terms: D000109:Acetylcholine / Q000494:pharmacology; D000804:Angiotensin II / Q000008:administration & dosage*; D000818:Animals; D001011:Aorta / Q000187:drug effects / Q000276:immunology / Q000503:physiopathology*; D001794:Blood Pressure; D015924:Blood Pressure Monitors; D002469:Cell Separation / Q000379:methods; D004334:Drug Administration Schedule; D004730:Endothelium, Vascular / Q000187:drug effects / Q000276:immunology / Q000503:physiopathology*; D006973:Hypertension / Q000139:chemically induced / Q000276:immunology / Q000503:physiopathology*; D015918:Infusion Pumps, Implantable; D008264:Macrophages / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D051379:Mice; D009000:Monocytes / Q000187:drug effects / Q000276:immunology / Q000473:pathology; D009924:Organ Culture Techniques; D009997:Osmotic Pressure; D014657:Vasculitis / Q000139:chemically induced / Q000276:immunology / Q000503:physiopathology*
chemical_list: D000804:Angiotensin II; D000109:Acetylcholine
keywords: Endothelial dysfunction; Inflammation; Vascular dysfunction