title: [Main regulatory element (MRE) of the Danio rerio α/β-globin gene domain exerts enhancer activity toward the promoters of the embryonic-larval and adult globin genes].
abstract: In warm-blooded vertebrates, the α- and β-globin genes are organized in domains of different types and are regulated in different fashion. In cold-blooded vertebrates and, in particular, the tropical fish Danio rerio, the α- and β-globin genes form two gene clusters. A major D. rerio globin gene cluster is in chromosome 3 and includes the α- and β-globin genes of embryonic-larval and adult types. The region upstream of the cluster contains c16orf35, harbors the main regulatory element (MRE) of the α-globin gene domain in warm-blooded vertebrates. In this study, transient transfection of erythroid cells with genetic constructs containing a reporter gene under the control of potential regulatory elements of the domain was performed to characterize the promoters of the embryonic-larval and adult α- and β-globin genes of the major cluster. Also, in the 5th intron of c16orf35 in Danio reriowas detected a functional analog of the warm-blooded vertebrate MRE. This enhancer stimulated activity of the promoters of both adult and embryonic-larval α- and β-globin genes.
authors.affiliation: Biological Faculty, Moscow State University, Moscow, 119992 Russia.|anastasiya.kovina@mail.ru.
authors.forename: A P
authors.initials: AP
authors.lastname: Kovina
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: N V
authors.initials: NV
authors.lastname: Petrova
authors.affiliation: Biological Faculty, Moscow State University, Moscow, 119992 Russia.|Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: S V
authors.initials: SV
authors.lastname: Razin
authors.affiliation: Institute of Gene Biology, Russian Academy of Sciences, Moscow, 119334 Russia.
authors.forename: O V
authors.initials: OV
authors.lastname: Yarovaia
mesh_terms: D000818:Animals; D004742:Enhancer Elements, Genetic; D011401:Promoter Regions, Genetic; D015027:Zebrafish / Q000196:embryology / Q000235:genetics*; D055542:alpha-Globins / Q000096:biosynthesis / Q000235:genetics*; D055544:beta-Globins / Q000096:biosynthesis / Q000235:genetics*
chemical_list: D055542:alpha-Globins; D055544:beta-Globins
keywords: Danio rerio; enhancer; globin genes; promoter