title: Direct digestion of living cells via a gel-based strategy for mass spectrometric analysis.
abstract: A novel sample preparation method was established for proteomic analysis, during which living cells were absorbed into vacuum-dried polyacrylamide gel and directly digested into peptides for subsequent LC-MS/MS assays. As a consequence, both of the steps for cell lysis and protein extraction involved in a conventional digestion method were skipped.
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P. R. China. luhaojie@fudan.edu.cn and Department of Chemistry, Fudan University, Shanghai 200433, P. R. China.
authors.forename: Yun
authors.initials: Y
authors.lastname: Xiong
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P. R. China. luhaojie@fudan.edu.cn.
authors.forename: Ying
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P. R. China. luhaojie@fudan.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Yao
authors.affiliation: Department of Chemistry, Fudan University, Shanghai 200433, P. R. China.
authors.forename: Guoquan
authors.initials: G
authors.lastname: Yan
authors.affiliation: Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, P. R. China. luhaojie@fudan.edu.cn and Department of Chemistry, Fudan University, Shanghai 200433, P. R. China.
authors.forename: Haojie
authors.identifier: http://orcid.org/0000-0003-3477-7662
authors.initials: H
authors.lastname: Lu
mesh_terms: D000180:Acrylic Resins / Q000737:chemistry*; D002477:Cells / Q000378:metabolism*; D002853:Chromatography, Liquid; D006801:Humans; D010455:Peptides / Q000737:chemistry / Q000378:metabolism*; D040901:Proteomics; D053719:Tandem Mass Spectrometry
chemical_list: D000180:Acrylic Resins; D010455:Peptides; C016680:polyacrylamide gels