title: Transplantation of human umbilical cord mesenchymal stem cells in cochlea to repair sensorineural hearing.
abstract: To examine if transplantation of human umbilical cord mesenchymal stem cells (UMSC) into cochlea can be used to repair sensorineural hearing. Here we transplanted the fifth and sixth generations of UMSCs through the subarachnoid cavity of congenital deaf albino pigs. Auditory brainstem responses (ABR) were measured before and after UMSC transplantation. Cochlear samples were collected at 2 hrs, 3 days, 1, 2, 3, 4 and 8 weeks after transplantation. Immunohistochemistry was used to detect the proliferated cell nuclear antigen (PCNA). The UMSCs were found in different regions of the cochlea, including the stria vascularis, the basal membrane and the spiral ganglions, 3 days to 4 weeks after the transplantation. UMSCs and their DNA were found also in the areas of the brain, the heart, the liver, the kidney and the lung. ABR tests displayed a new waveform in the congenital deaf albino pigs after the UMSCs transplantation. We conclude that human UMSCs injected into the subarachnoid space can migrate into the inner ear, the central nervous system and the periphery organs. The presence of UMSCs in the cochlea maybe associated with changes of ABR waveforms in the congenital deaf albino pigs.
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Beijing Friendship Hospital, Capital Medical UniversityBeijing, China; Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General HospitalBeijing, China.
authors.forename: Yueying
authors.initials: Y
authors.lastname: Ma
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Weiwei
authors.initials: W
authors.lastname: Guo
authors.affiliation: Department of Otolaryngology, Head & Neck Surgery, Beijing Tsinghua Changgung Hospital, Medical center, Tsinghua University 168# Litang Street, Changping District, Beijing, China.
authors.forename: Haijin
authors.initials: H
authors.lastname: Yi
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Ren
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Lidong
authors.initials: L
authors.lastname: Zhao
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Shuolong
authors.initials: S
authors.lastname: Yuan
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Riyuan
authors.initials: R
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Liangwei
authors.initials: L
authors.lastname: Xu
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Tao
authors.initials: T
authors.lastname: Cong
authors.affiliation: Department of Chemical Pathology, Faculty of Basic & Allied Medical Sciences, College of Heath Sciences, Benue State University Makurdi, Nigeria.
authors.forename: Oghagbon
authors.initials: O
authors.lastname: Ek
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Suoqiang
authors.initials: S
authors.lastname: Zhai
authors.affiliation: Department of Otolaryngology, Head and Neck Surgery, Chinese PLA General Hospital Beijing, China.
authors.forename: Shiming
authors.initials: S
authors.lastname: Yang
keywords: Human umbilical cord mesenchymal stem cells (UMSC); auditory brainstem response; pigs; subarachnoid cavity