title: Bone Healing Improvements Using Hyaluronic Acid and Hydroxyapatite/Beta-Tricalcium Phosphate in Combination: An Animal Study.
abstract: The purpose of this study was to investigate whether the use of HLA as an aqueous binder of hydroxyapatite/beta-tricalcium phosphate (HA-βTCP) particles can reduce the amount of bone graft needed and increase ease of handling in clinical situations. In this study, HA/βTCP was loaded in commercially available crosslinking HLA to form a novel HLA/HA-βTCP composite. Six New Zealand White rabbits (3.0-3.6 kg) were used as test subjects. Four 6 mm defects were prepared in the parietal bone. The defects were filled with the HLA/HA-βTCP composite as well as HA-βTCP particle alone. New bone formation was analyzed by micro-CT and histomorphometry. Our results indicated that even when the HA-βTCP particle numbers were reduced, the regenerative effect on bone remained when the HLA existed. The bone volume density (BV/TV ratio) of HLA/HA-βTCP samples was 1.7 times larger than that of the control sample at week 2. The new bone increasing ratio (NBIR) of HLA/HA-βTCP samples was 1.78 times higher than the control group at week 2. In conclusion, HA-βTCP powder with HLA contributed to bone healing in rabbit calvarial bone defects. The addition of HLA to bone grafts not only promoted osteoconduction but also improved handling characteristics in clinical situations.
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Dental Department, Mackey Memorial Hospital, Taipei, Taiwan.
authors.forename: Yen-Lan
authors.initials: YL
authors.lastname: Chang
authors.affiliation: Dental Department, Wan Fang Hospital, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yi-June
authors.initials: YJ
authors.lastname: Lo
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; School of Oral Hygiene, College of Oral Sciences, Taipei Medical University, Taipei, Taiwan.
authors.forename: Sheng-Wei
authors.identifier: 0000-0001-6637-7044
authors.initials: SW
authors.lastname: Feng
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Yu-Chih
authors.initials: YC
authors.lastname: Huang
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Hsin-Yuan
authors.initials: HY
authors.lastname: Tsai
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan.
authors.forename: Che-Tong
authors.initials: CT
authors.lastname: Lin
authors.affiliation: Dental Department, En Chu Kong Hospital, New Taipei City, Taiwan.
authors.forename: Kan-Hsin
authors.initials: KH
authors.lastname: Fan
authors.affiliation: School of Dentistry, College of Oral Medicine, Taipei Medical University, Taipei, Taiwan; Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei, Taiwan.
authors.forename: Haw-Ming
authors.identifier: 0000-0003-4184-3744
authors.initials: HM
authors.lastname: Huang
mesh_terms: D000818:Animals; D001847:Bone Diseases / Q000628:therapy*; D001861:Bone Regeneration; D018786:Bone Substitutes / Q000737:chemistry*; D001842:Bone and Bones / Q000000981:diagnostic imaging* / Q000473:pathology* / Q000601:surgery; D004195:Disease Models, Animal; D006882:Hydroxyapatites / Q000737:chemistry*; D010012:Osteogenesis; D011208:Powders; D011817:Rabbits; D012886:Skull / Q000473:pathology; D055114:X-Ray Microtomography
chemical_list: D018786:Bone Substitutes; D006882:Hydroxyapatites; D011208:Powders; C074950:hydroxyapatite-beta tricalcium phosphate