title: Recovery of Lactobacillus casei strain Shirota (LcS) from the intestine of healthy Vietnamese adults after intake of fermented milk.
abstract: To demonstrate the gastrointestinal survival of Lactobacillus casei strain Shirota (LcS) in healthy Vietnamese adults, a fermented milk drink containing LcS was administered daily for 14 days. Twenty-six healthy Vietnamese adults took part in the study. Each participant consumed 65 mL of a fermented milk drink containing LcS daily for 14 days. The drink contained a dose of 10 8 CFU/mL LcS. Fecal samples were collected before, during and after consuming the fermented milk drink. LcS was confirmed by culture and ELISA. After 7 and 14 days of ingesting fermented milk drink, LcS was recovered from fecal samples at average of 5.0×10 7 CFU/g feces (n=26) and 5.4×10 7 CFU/g feces (n=26), respectively. LcS persisted in 8 voluteers until day 42 (after 14 days stopping fermented milk drink) at 0.0033×10 7 CFU/g feces (n=8). We confirmed survival of LcS after passage through the gastrointestinal tract of Vietnamese adults.
authors.affiliation: National Institute of Nutrition, Vietnam. Email: truongmai1976@yahoo.com; truongmai1976@gmail.com.|Institute of Microbiology and Biotechnology, Vietnam.
authors.forename: Truong Tuyet
authors.initials: TT
authors.lastname: Mai
authors.affiliation: Institute of Microbiology and Biotechnology, Vietnam.
authors.forename: Duong Van
authors.initials: DV
authors.lastname: Hop
authors.affiliation: National Institute of Nutrition, Vietnam.
authors.forename: Trinh Thi Van
authors.initials: TT
authors.lastname: Anh
authors.affiliation: National Institute of Nutrition, Vietnam.
authors.forename: Nguyen Thi
authors.initials: NT
authors.lastname: Lam
mesh_terms: D000293:Adolescent; D000328:Adult; D015169:Colony Count, Microbial; D003248:Constipation / Q000628:therapy; D043302:Cultured Milk Products / Q000009:adverse effects / Q000382:microbiology*; D003672:Defecation / Q000502:physiology; D005243:Feces / Q000382:microbiology; D005260:Female; D005772:Gastrointestinal Transit; D006801:Humans; D007422:Intestines / Q000382:microbiology*; D007780:Lacticaseibacillus casei / Q000302:isolation & purification* / Q000502:physiology; D008297:Male; D019936:Probiotics; D014744:Vietnam; D055815:Young Adult