title: Interaction of the CD43 Sialomucin with the Mycobacterium tuberculosis Cpn60.2 Chaperonin Leads to Tumor Necrosis Factor Alpha Production.
abstract: Mycobacterium tuberculosisis the causal agent of tuberculosis. Tumor necrosis factor alpha (TNF-α), transforming growth factor β (TGF-β), and gamma interferon (IFN-γ) secreted by activated macrophages and lymphocytes are considered essential to containMycobacterium tuberculosisinfection. The CD43 sialomucin has been reported to act as a receptor for bacilli through its interaction with the chaperonin Cpn60.2, facilitating mycobacterium-macrophage contact. We report here that Cpn60.2 induces both human THP-1 cells and mouse-derived bone marrow-derived macrophages (BMMs) to produce TNF-α and that this production is CD43 dependent. In addition, we present evidence that the signaling pathway leading to TNF-α production upon interaction with Cpn60.2 requires active Src family kinases, phospholipase C-γ (PLC-γ), phosphatidylinositol 3-kinase (PI3K), p38, and Jun N-terminal protein kinase (JNK), both in BMMs and in THP-1 cells. Our data highlight the role of CD43 and Cpn60.2 in TNF-α production and underscore an important role for CD43 in the host-mycobacterium interaction.
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Cuernavaca, Morelos, Mexico.|Posgrado en Ciencias, Universidad Autónoma del Estado de Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Alvaro
authors.initials: A
authors.lastname: Torres-Huerta
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Tomás
authors.initials: T
authors.lastname: Villaseñor
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Angel
authors.initials: A
authors.lastname: Flores-Alcantar
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Cristina
authors.initials: C
authors.lastname: Parada
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Cuernavaca, Morelos, Mexico.|Posgrado en Ciencias Bioquímicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Estefanía
authors.initials: E
authors.lastname: Alemán-Navarro
authors.affiliation: Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, Mexico City, Mexico.
authors.forename: Clara
authors.initials: C
authors.lastname: Espitia
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Cuernavaca, Morelos, Mexico.
authors.forename: Gustavo
authors.initials: G
authors.lastname: Pedraza-Alva
authors.affiliation: Instituto de Biotecnología, Universidad Nacional Autónoma de México, Campus Morelos, Cuernavaca, Morelos, Mexico yvonne@ibt.unam.mx.
authors.forename: Yvonne
authors.initials: Y
authors.lastname: Rosenstein
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002460:Cell Line; D018834:Chaperonin 60 / Q000378:metabolism*; D006801:Humans; D051917:Leukosialin / Q000378:metabolism*; D008264:Macrophages / Q000276:immunology / Q000378:metabolism / Q000382:microbiology; D009169:Mycobacterium tuberculosis / Q000502:physiology*; D016328:NF-kappa B / Q000378:metabolism; D011485:Protein Binding; D015398:Signal Transduction; D018808:Transcription Factor AP-1 / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000096:biosynthesis*
chemical_list: D001426:Bacterial Proteins; D018834:Chaperonin 60; D051917:Leukosialin; D016328:NF-kappa B; D018808:Transcription Factor AP-1; D014409:Tumor Necrosis Factor-alpha
keywords: CD43; Cpn60.2; Mycobacterium tuberculosis; cell signaling; macrophages; tumor necrosis factor alpha