title: Porcine GPX1 enhances GP5-based DNA vaccination against porcine reproductive and respiratory syndrome virus.
abstract: Porcine reproductive and respiratory syndrome virus (PRRSV) has been causing huge economic losses to the swine industry worldwide. Commercial PRRSV vaccines including killed and modified live ones are available. However the protective efficacy of these vaccines is incomplete. Thus, it is in urgent need to develop safer and more effective PRRSV vaccines. In this study, we constructed a recombinant plasmid co-expressing porcine glutathione peroxidase-1 (GPX1) and the envelope glycoprotein (GP5) encoding-gene of PRRSV (pcDNA3.1-GPX1-LSynORF5), and investigated the immune response induced following inoculation of mice and piglets. Significantly enhanced GP5-specific antibody, PRRSV-specific neutralizing antibody, IFN-γ level, as well as lymphocyte proliferation response, were induced in mice and pigs immunized with the DNA construct encoding GPX1 and GP5 compared with those inoculated with a construct encoding PRRSV GP5 only (pcDNA3.1-SynORF5). The enhanced cellular immune response in pigs induced by pcDNA3.1-GPX1-LSynORF5 was comparable to that induced by the attenuated virus vaccine JXA1-R, although the humoral immune response induced by the plasmid was much lower than the response induced by JXA1-R. Following the challenge with highly pathogenic PRRSV, less-severe clinical signs and rectal temperatures were observed in pigs immunized with the GPX1-GP5 construct compared with the control group. However, the viraemia of groups immunized with plasmid was more severe than that inoculated with JXA1-R, and it is likely that this could be attributed to the poor humoral response induced by the GPX1-GP5 construct. These results demonstrated that inclusion of GPX1 in a PRRSV DNA vaccine leads to an adjuvant effect, enhancing the humoral and cellular responses following vaccination.
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-gang, Nanjing 210095, China; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China; Jiangsu Co-infection Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 Jiangsu Province, China.
authors.forename: Luping
authors.initials: L
authors.lastname: Du
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China; Jiangsu Co-infection Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 Jiangsu Province, China.
authors.forename: Bin
authors.initials: B
authors.lastname: Li
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-gang, Nanjing 210095, China; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China.
authors.forename: Fengjiao
authors.initials: F
authors.lastname: Pang
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China; Jiangsu Co-infection Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 Jiangsu Province, China.
authors.forename: Zhengyu
authors.initials: Z
authors.lastname: Yu
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-gang, Nanjing 210095, China; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China.
authors.forename: Xiangwei
authors.initials: X
authors.lastname: Xu
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China; Jiangsu Co-infection Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 Jiangsu Province, China.
authors.forename: Baochao
authors.initials: B
authors.lastname: Fan
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China; Jiangsu Co-infection Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 Jiangsu Province, China.
authors.forename: Yeping
authors.initials: Y
authors.lastname: Tan
authors.affiliation: Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing, 210014 Jiangsu Province, China; Jiangsu Co-infection Center for Prevention and Control of Important Animal Infectious Disease and Zoonoses, Yangzhou, 225009 Jiangsu Province, China.
authors.forename: Kongwang
authors.initials: K
authors.lastname: He
authors.affiliation: College of Veterinary Medicine, Nanjing Agricultural University, No. 1 Wei-gang, Nanjing 210095, China. Electronic address: khhuang@njau.edu.cn.
authors.forename: Kehe
authors.initials: K
authors.lastname: Huang
mesh_terms: D000818:Animals; D001831:Body Temperature; D002522:Chlorocebus aethiops; D004279:DNA, Viral; D005979:Glutathione Peroxidase / Q000235:genetics / Q000276:immunology*; D007111:Immunity, Cellular; D056724:Immunity, Humoral; D000071497:Immunogenicity, Vaccine; D051379:Mice; D008807:Mice, Inbred BALB C; D010957:Plasmids; D019318:Porcine Reproductive and Respiratory Syndrome / Q000276:immunology / Q000473:pathology / Q000517:prevention & control*; D019316:Porcine respiratory and reproductive syndrome virus / Q000235:genetics / Q000276:immunology; D013552:Swine; D013553:Swine Diseases / Q000276:immunology / Q000473:pathology / Q000517:prevention & control* / Q000821:virology; D013601:T-Lymphocytes / Q000276:immunology; D019444:Vaccines, DNA / Q000008:administration & dosage* / Q000276:immunology; D014614:Vaccines, Synthetic / Q000008:administration & dosage / Q000276:immunology; D014709:Vero Cells; D014759:Viral Envelope Proteins / Q000235:genetics / Q000276:immunology*; D014765:Viral Vaccines / Q000008:administration & dosage* / Q000276:immunology; D014766:Viremia / Q000517:prevention & control; D000094765:Glutathione Peroxidase GPX1
chemical_list: D004279:DNA, Viral; D019444:Vaccines, DNA; D014614:Vaccines, Synthetic; D014759:Viral Envelope Proteins; D014765:Viral Vaccines; C114789:glycoprotein 5, PRRSV; D005979:Glutathione Peroxidase; D000094765:Glutathione Peroxidase GPX1
keywords: DNA vaccine; GPX1; Immune response; PRRSV