title: Localization of functional β-xylosidases, encoded by the same single gene, xlsIV (xlnD), from Aspergillus niger E-1.
abstract: Cell wall-associated β-xylosidase was isolated from Aspergillus niger E-1 and identified as XlsIV, corresponding to the extracellular enzyme XlnD reported previously. xlsIV was transcribed only in the early cultivation period. Cell wall-associated enzyme activity gradually decreased, but extracellular activity increased as the strain grew. These results indicate that XlsIV (XlnD) was secreted into culture after localizing at cell wall.
authors.affiliation: a Department of Agricultural Chemistry , School of Agriculture, Meiji University , Kawasaki , Japan.
authors.forename: Kotomi
authors.initials: K
authors.lastname: Inoue
authors.affiliation: a Department of Agricultural Chemistry , School of Agriculture, Meiji University , Kawasaki , Japan.
authors.forename: Yui
authors.initials: Y
authors.lastname: Takahashi
authors.affiliation: a Department of Agricultural Chemistry , School of Agriculture, Meiji University , Kawasaki , Japan.
authors.forename: Ken
authors.initials: K
authors.lastname: Obara
authors.affiliation: a Department of Agricultural Chemistry , School of Agriculture, Meiji University , Kawasaki , Japan.
authors.forename: Shuichiro
authors.initials: S
authors.lastname: Murakami
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology* / Q000235:genetics / Q000254:growth & development; D002473:Cell Wall / Q000378:metabolism; D005656:Fungal Proteins / Q000235:genetics / Q000378:metabolism*; D014995:Xylosidases / Q000235:genetics / Q000378:metabolism*
chemical_list: D005656:Fungal Proteins; D014995:Xylosidases
keywords: Aspergillus niger; localization; xlnD; β-xylosidases