title: Quick Histochemical Staining Methods to Detect Cell Death in Xylem Elements of Plant Tissues.
abstract: Histochemical assays of xylem cell death cannot take advantage of the conventional methods for detection of cell death, such as staining with propidium iodide or trypan/Evans blue or the TUNEL staining. This chapter presents two alternative histochemical methods that can be used to detect xylem cell death quickly and reliably using light microscopy. The first method is a viability stain that can be used to detect cell death of different types of xylem elements in basically any plant species. The second method reveals cell death in xylem vessel elements based on their functionality in transport of water and small water-soluble stains.
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187, Umeå, Sweden.
authors.forename: Sacha
authors.initials: S
authors.lastname: Escamez
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187, Umeå, Sweden.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Bollhöner
authors.affiliation: Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, 90187, Umeå, Sweden. Hannele.tuominen@umu.se.
authors.forename: Hannele
authors.initials: H
authors.lastname: Tuominen
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000378:metabolism; D001692:Biological Transport; D016923:Cell Death; D002470:Cell Survival; D006651:Histocytochemistry / Q000379:methods; D008856:Microscopy, Fluorescence; D052584:Xylem / Q000166:cytology* / Q000378:metabolism*
keywords: Arabidopsis; Libriform fiber; Nitroblue tetrazolium; Populus; Programmed cell death; Sulforhodamine; Tracheary element; Viability stain; Wood; Xylem