title: Serum Cytokine Profiles in Children with Crohn's Disease.
abstract: Crohn's disease (CD) is a chronic inflammatory bowel disease that can be diagnosed at any age. There are two major patient groups based on diagnosis of this disease, before or after the age of 20 (juvenile/adolescent or adult), with disease progression in adults usually milder than in juvenile CD patients. Immune mechanisms have been suggested to play an important role in CD pathogenesis, with cytokines governing the development of the immune response. Upregulation of inflammatory cytokines in serum of juvenile and adult CD patients has been documented; still little is known about age-dependent differences in serum cytokine profiles of CD patients. We applied multiplex technology to analyze serum levels of 12 cytokines in juveniles and adults. We show that during the acute stage of the disease all CD patients have high serum levels of CXCL10, which remains upregulated during remission. Increased serum levels of TNF-αand IL-6 during the acute stage was characteristic of juvenile CD patients, whereas adult CD patients had upregulated levels of GM-CSF and IFN-γ. Taken together, these results demonstrate age-dependent differences in cytokine profiles, which may affect the pathogenesis of CD in patients at different ages of disease onset.
authors.affiliation: Volga Region Federal Medical Research Center of the Ministry of Public Health, Nizhny Novgorod, Russia.
authors.forename: Ekaterina
authors.initials: E
authors.lastname: Vasilyeva
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia; Kazan Federal University Clinic, Kazan, Russia.
authors.forename: Sayar
authors.initials: S
authors.lastname: Abdulkhakov
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia; Kazan Federal University Clinic, Kazan, Russia; Department of Clinical Laboratory Diagnostics, Kazan State Medical Academy, Kazan, Russia.
authors.forename: Georgi
authors.initials: G
authors.lastname: Cherepnev
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
authors.forename: Ekaterina
authors.identifier: 0000-0003-1537-3099
authors.initials: E
authors.lastname: Martynova
authors.affiliation: Volga Region Federal Medical Research Center of the Ministry of Public Health, Nizhny Novgorod, Russia.
authors.forename: Irina
authors.initials: I
authors.lastname: Mayanskaya
authors.affiliation: Kazan State Medical University, Kazan, Russia.
authors.forename: Alina
authors.initials: A
authors.lastname: Valeeva
authors.affiliation: Kazan State Medical University, Kazan, Russia.
authors.forename: Rustam
authors.initials: R
authors.lastname: Abdulkhakov
authors.affiliation: Kazan State Medical University, Kazan, Russia.
authors.forename: Dilyara
authors.initials: D
authors.lastname: Safina
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia; Department of Microbiology and Immunology, University of Nevada Reno, Reno, NV, USA.
authors.forename: Svetlana
authors.identifier: 0000-0002-5064-570X
authors.initials: S
authors.lastname: Khaiboullina
authors.affiliation: Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.
authors.forename: Albert
authors.identifier: 0000-0002-9427-5739
authors.initials: A
authors.lastname: Rizvanov
mesh_terms: D000208:Acute Disease; D000293:Adolescent; D000328:Adult; D054357:Chemokine CXCL10 / Q000097:blood; D002648:Child; D003424:Crohn Disease / Q000097:blood*; D016207:Cytokines / Q000097:blood*; D018450:Disease Progression; D005260:Female; D016178:Granulocyte-Macrophage Colony-Stimulating Factor / Q000097:blood; D006801:Humans; D007107:Immune System; D007249:Inflammation; D007371:Interferon-gamma / Q000097:blood; D015850:Interleukin-6 / Q000097:blood; D008297:Male; D008875:Middle Aged; D012074:Remission Induction; D014409:Tumor Necrosis Factor-alpha / Q000097:blood; D055815:Young Adult
chemical_list: C114753:CXCL10 protein, human; D054357:Chemokine CXCL10; D016207:Cytokines; C573415:IFNG protein, human; C508600:IL6 protein, human; D015850:Interleukin-6; D014409:Tumor Necrosis Factor-alpha; D007371:Interferon-gamma; D016178:Granulocyte-Macrophage Colony-Stimulating Factor