title: A SUMO-ubiquitin relay recruits proteasomes to chromosome axes to regulate meiotic recombination.
abstract: Meiosis produces haploid gametes through a succession of chromosomal events, including pairing, synapsis, and recombination. Mechanisms that orchestrate these events remain poorly understood. We found that the SUMO (small ubiquitin-like modifier)-modification and ubiquitin-proteasome systems regulate the major events of meiotic prophase in mouse. Interdependent localization of SUMO, ubiquitin, and proteasomes along chromosome axes was mediated largely by RNF212 and HEI10, two E3 ligases that are also essential for crossover recombination. RNF212-dependent SUMO conjugation effected a checkpointlike process that stalls recombination by rendering the turnover of a subset of recombination factors dependent on HEI10-mediated ubiquitylation. We propose that SUMO conjugation establishes a precondition for designating crossover sites via selective protein stabilization. Thus, meiotic chromosome axes are hubs for regulated proteolysis via SUMO-dependent control of the ubiquitin-proteasome system.
authors.affiliation: Howard Hughes Medical Institute, University of California, Davis, CA 95616, USA.|Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: H B D Prasada
authors.initials: HB
authors.lastname: Rao
authors.affiliation: Howard Hughes Medical Institute, University of California, Davis, CA 95616, USA.|Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Huanyu
authors.initials: H
authors.lastname: Qiao
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Shubhang K
authors.initials: SK
authors.lastname: Bhatt
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Logan R J
authors.initials: LR
authors.lastname: Bailey
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Hung D
authors.initials: HD
authors.lastname: Tran
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Sarah L
authors.initials: SL
authors.lastname: Bourne
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Wendy
authors.initials: W
authors.lastname: Qiu
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Anusha
authors.initials: A
authors.lastname: Deshpande
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Ajay N
authors.initials: AN
authors.lastname: Sharma
authors.affiliation: Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
authors.forename: Connor J
authors.initials: CJ
authors.lastname: Beebout
authors.affiliation: Cell Cycle and Cancer Biology Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA.
authors.forename: Roberto J
authors.initials: RJ
authors.lastname: Pezza
authors.affiliation: Howard Hughes Medical Institute, University of California, Davis, CA 95616, USA. nhunter@ucdavis.edu.|Department of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.|Department of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.|Department of Cell Biology and Human Anatomy, University of California, Davis, CA 95616, USA.
authors.forename: Neil
authors.initials: N
authors.lastname: Hunter
mesh_terms: D000818:Animals; D018797:Cell Cycle Proteins; D023902:Chromosome Pairing; D033481:Chromosomes, Mammalian / Q000378:metabolism; D003434:Crossing Over, Genetic / Q000235:genetics / Q000502:physiology*; D008025:Ligases / Q000235:genetics / Q000378:metabolism*; D008297:Male; D008540:Meiosis / Q000235:genetics / Q000502:physiology*; D051379:Mice; D008817:Mice, Mutant Strains; D046988:Proteasome Endopeptidase Complex / Q000378:metabolism*; D059748:Proteolysis; D025841:Small Ubiquitin-Related Modifier Proteins / Q000378:metabolism*; D013090:Spermatocytes / Q000166:cytology / Q000378:metabolism; D025801:Ubiquitin / Q000378:metabolism; D044767:Ubiquitin-Protein Ligases / Q000235:genetics / Q000378:metabolism*; D054875:Ubiquitination
chemical_list: D018797:Cell Cycle Proteins; D025841:Small Ubiquitin-Related Modifier Proteins; D025801:Ubiquitin; C523697:Ccnb1ip1 protein, mouse; D044767:Ubiquitin-Protein Ligases; D046988:Proteasome Endopeptidase Complex; D008025:Ligases; C581307:Rnf212 protein, mouse