title: Optimization, characterization, and in vitro assessment of alginate-pectin ionic cross-linked hydrogel film for wound dressing applications.
abstract: Natural polymer-based hydrogel films have great potential for biomedical applications and are good candidates for wound dressings. In this study, we aimed to develop simvastatin-loaded crosslinked alginate-pectin hydrogel films by ionic crosslinking to improve the mechanical characteristics, wound fluid uptake and drug release behavior. Alginate-pectin hydrocolloid films were chemically crosslinked by immersing in different concentrations of CaCl2(0.5-3% w/v) for 2-20min. The degree of crosslinking was influenced by both contact time and CaCl2concentration. The optimized conditions for crosslinking were 0.5% and 1% (CaCl2) for 2min. The optimized hydrogel films were then characterized for their physical, mechanical, morphological, thermal, in vitro drug release, and cytocompatibility profiles. Crosslinking improved the mechanical profile and wound fluid uptake capacity of dressings. The hydrogel films were able to maintain their physical integrity during use, and the best results were obtained with the film in which the extent of crosslinking was low (0.5%). Thermal analysis confirmed that the crosslinking process enhanced the thermal stability of hydrogel films. Sustained, slow release of simvastatin was obtained from the crosslinked films and in vitro cytotoxicity assay demonstrated that the hydrogel films were non-toxic.
authors.affiliation: Centre of Drug Delivery Research, Faculty of Pharmacy, University Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
authors.forename: Masoud
authors.initials: M
authors.lastname: Rezvanian
authors.affiliation: Department of Pharmacy, Forman Christian College (A Chartered University), Ferozepur Road 54600, Lahore, Pakistan.
authors.forename: Naveed
authors.initials: N
authors.lastname: Ahmad
authors.affiliation: Centre of Drug Delivery Research, Faculty of Pharmacy, University Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia.
authors.forename: Mohd Cairul Iqbal
authors.initials: MC
authors.lastname: Mohd Amin
authors.affiliation: Centre of Drug Delivery Research, Faculty of Pharmacy, University Kebangsaan Malaysia, Jalan Raja Muda Abdul Aziz, 50300, Kuala Lumpur, Malaysia. Electronic address: nsfern@gmail.com.
authors.forename: Shiow-Fern
authors.initials: SF
authors.lastname: Ng
mesh_terms: D000464:Alginates / Q000737:chemistry*; D001458:Bandages; D002122:Calcium Chloride / Q000737:chemistry; D004337:Drug Carriers / Q000737:chemistry* / Q000494:pharmacology; D065546:Drug Liberation; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D020723:Glucuronic Acid / Q000737:chemistry; D006603:Hexuronic Acids / Q000737:chemistry; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D007700:Kinetics; D008422:Materials Testing; D055595:Mechanical Phenomena; D010368:Pectins / Q000737:chemistry*; D019821:Simvastatin / Q000737:chemistry / Q000494:pharmacology; D012867:Skin / Q000166:cytology; D013227:Steam; D013696:Temperature; D014945:Wound Healing / Q000187:drug effects
chemical_list: D000464:Alginates; D004337:Drug Carriers; D006603:Hexuronic Acids; D020100:Hydrogels; D013227:Steam; D010368:Pectins; D020723:Glucuronic Acid; D019821:Simvastatin; D002122:Calcium Chloride
keywords: Alginate; Hydrogel film; Pectin; Simvastatin; Wound dressing