title: Transcriptomic Analysis of Calonectria pseudoreteaudii during Various Stages of Eucalyptus Infection.
abstract: Eucalyptus leaf blight caused by Calonectria spp. is a serious disease in Eucalyptus seedling and plantations. However, the molecular mechanisms of the infection process and pathogenesis of Calonectria to Eucalyptus is not well-studied. In this study, we analyzed the transcriptomes of C. pseudoreteaudii at three stages of Eucalyptus leaf infection, and in mycelium grown in potato dextrose broth using Illumina RNA-Seq technology. We identified 161 differentially expressed genes between C. pseudoreteaudii from leaf and mycelium grown in potato dextrose broth. GO and KEGG enrichment analyses of these genes suggested that they were mainly involved in oxidoreductase activity, hydrolase activity, and transmembrane transporter activity. Most of the differentially expressed genes at the early infection stage were upregulated. These upregulated genes were mainly involved in cell wall hydrolysis and toxin synthesis, suggesting a role for toxin and cell wall hydrolases in the establishment of Calonectria leaf blight. Genes related to detoxification of phytoalexins were continually upregulated during infection. The candidate effectors and putative pathogenicity determinants identified in this study will help in the functional analysis of C. pseudoreteaudii virulence and pathogenicity.
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.|Jinshan College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Xiaozhen
authors.initials: X
authors.lastname: Ye
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Hongyi
authors.initials: H
authors.lastname: Liu
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Yajie
authors.initials: Y
authors.lastname: Jin
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Mengmeng
authors.initials: M
authors.lastname: Guo
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Aizhen
authors.initials: A
authors.lastname: Huang
authors.affiliation: Jinshan College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Quanzhu
authors.initials: Q
authors.lastname: Chen
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Wenshuo
authors.initials: W
authors.lastname: Guo
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Feiping
authors.initials: F
authors.lastname: Zhang
authors.affiliation: Forestry College, Fujian Agriculture and Forestry University, Fuzhou, China.|Institute of Forestry Protection, Fujian Agriculture and Forestry University, Fuzhou, China.
authors.forename: Lizhen
authors.initials: L
authors.lastname: Feng
mesh_terms: D001203:Ascomycota / Q000235:genetics* / Q000648:ultrastructure; D019295:Computational Biology / Q000379:methods; D005052:Eucalyptus / Q000382:microbiology*; D020869:Gene Expression Profiling; D015966:Gene Expression Regulation, Fungal; D063990:Gene Ontology; D058977:Molecular Sequence Annotation; D010935:Plant Diseases / Q000382:microbiology*; D059467:Transcriptome