title: Evaluation of a New Biosensor Based on in Situ Synthesized PPy-Ag-PVP Nanohybrid for Selective Detection of Dopamine.
abstract: In the present work, in situ synthesis of polypyrrole-silver-polyvinylpyrrolidone (PPy-Ag-PVP) nanohybrid using AgNO3as an oxidant and polyvinylpyrrolidone (PVP) as a stabilizer and surfactant is demonstrated. The obtained ternary PPy-Ag-PVP nanohybrid was characterized by UV-vis, FT-IR, XRD, Raman, TGA, SEM, and HR-TEM analysis. Further the synthesized PPy-Ag-PVP has been investigated for its selective and sensitive sensing of dopamine (DA). The PPy-Ag-PVP modified glassy carbon electrode shows a reversible electrochemical behavior with superior response for DA. The limit of detection and limit of quantification are found to be 0.0126 and 0.042 μM (S/N = 3 and 10), respectively, with remarkable sensitivity (7.26 μA mM-1cm-2). The practical application of the present modified electrode has been validated by determining the concentration of DA in human urine samples of different age group.
authors.affiliation: Department of Chemistry, Thiagarajar College , Madurai 625 009, Tamil Nadu, India.
authors.forename: Balakumar
authors.initials: B
authors.lastname: Vellaichamy
authors.affiliation: Department of Chemistry, Thiagarajar College , Madurai 625 009, Tamil Nadu, India.
authors.forename: Prakash
authors.identifier: 0000-0001-5316-009X
authors.initials: P
authors.lastname: Periakaruppan
authors.affiliation: Department of Chemistry, Thiagarajar College , Madurai 625 009, Tamil Nadu, India.
authors.forename: Tharmaraj
authors.initials: T
authors.lastname: Paulmony
mesh_terms: D015374:Biosensing Techniques; D002244:Carbon / Q000737:chemistry; D004298:Dopamine / Q000032:analysis*; D055664:Electrochemical Techniques; D004566:Electrodes; D015394:Molecular Structure; D053761:Nanocomposites / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D011205:Povidone / Q000737:chemistry*; D011758:Pyrroles / Q000737:chemistry*; D012835:Silver Nitrate / Q000737:chemistry*
chemical_list: D011108:Polymers; D011758:Pyrroles; C067635:polypyrrole; D002244:Carbon; D012835:Silver Nitrate; D011205:Povidone; D004298:Dopamine