title: Sequence specific sorting of DNA molecules with FACS using 3dPCR.
abstract: Genetic heterogeneity is an important feature of many biological systems, but introduces technical challenges to their characterization. Even with the best modern instruments, only a small fraction of DNA molecules present in a sample can be read, and they are recovered in the form of short, hundred-base reads. In this paper, we introduce 3dPCR, a method to sort DNA molecules with sequence specificity. 3dPCR allows heterogeneous populations of DNA to be sorted to recover long targets for deep sequencing. It is valuable whenever a target sequence is rare in a mixed population, such as for characterizing mutations in heterogeneous cancer cell populations or identifying cells containing a specific genetic sequence or infected with a target virus.
authors.affiliation: Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California, USA.|California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, California, USA.
authors.forename: David J
authors.initials: DJ
authors.lastname: Sukovich
authors.affiliation: Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California, USA.|California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, California, USA.|UC Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Shea T
authors.initials: ST
authors.lastname: Lance
authors.affiliation: Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California, USA.|California Institute for Quantitative Biosciences, University of California San Francisco, San Francisco, California, USA.|UC Berkeley-UCSF Graduate Program in Bioengineering, University of California San Francisco, San Francisco, CA, USA.
authors.forename: Adam R
authors.initials: AR
authors.lastname: Abate
mesh_terms: D004247:DNA / Q000145:classification* / Q000235:genetics*; D005434:Flow Cytometry / Q000379:methods*; D016133:Polymerase Chain Reaction / Q000379:methods*
chemical_list: D004247:DNA