title: Autophagy-related protein MoAtg14 is involved in differentiation, development and pathogenicity in the rice blast fungus Magnaporthe oryzae.
abstract: Autophagy is the major intracellular degradation system by which cytoplasmic materials are delivered to and degraded in the vacuole/lysosome in eukaryotic cells. MoAtg14 in M. oryzae, a hitherto uncharacterized protein, is the highly divergent homolog of the yeast Atg14 and the mammal BARKOR. The MoATG14 deletion mutant exhibited collapse in the center of the colonies, poor conidiation and a complete loss of virulence. Significantly, the ΔMoatg14 mutant showed delayed breakdown of glycogen, less lipid bodies, reduced turgor pressure in the appressorium and impaired conidial autophagic cell death. The autophagic process was blocked in the ΔMoatg14 mutant, and the autophagic degradation of the marker protein GFP-MoAtg8 was interrupted. GFP-MoAtg14 co-localized with mCherry-MoAtg8 in the aerial hypha. In addition, a conserved coiled-coil domain was predicted in the N-terminal region of the MoAtg14 protein, a domain which could mediate the interaction between MoAtg14 and MoAtg6. The coiled-coil domain of the MoAtg14 protein is essential for its function in autophagy and pathogenicity.
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Xiao-Hong
authors.initials: XH
authors.lastname: Liu
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Ya-Hui
authors.initials: YH
authors.lastname: Zhao
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Xue-Ming
authors.initials: XM
authors.lastname: Zhu
authors.affiliation: State Intellectual Property Office of the People's Republic of China, Beijing, 100080, China.
authors.forename: Xiao-Qing
authors.initials: XQ
authors.lastname: Zeng
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Lu-Yao
authors.initials: LY
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Dong
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, 310058, China.|Agricultural Technology Extension Center, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Zhen-Zhu
authors.initials: ZZ
authors.lastname: Su
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Yao
authors.initials: Y
authors.lastname: Wang
authors.affiliation: College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Jian-Ping
authors.initials: JP
authors.lastname: Lu
authors.affiliation: State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou, 310058, China.
authors.forename: Fu-Cheng
authors.initials: FC
authors.lastname: Lin
mesh_terms: D000595:Amino Acid Sequence; D001343:Autophagy; D000071183:Autophagy-Related Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005656:Fungal Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D006003:Glycogen / Q000378:metabolism; D025301:Hyphae / Q000254:growth & development / Q000378:metabolism; D066292:Lipid Droplets / Q000378:metabolism; D020082:Magnaporthe / Q000378:metabolism* / Q000472:pathogenicity; D016296:Mutagenesis; D012275:Oryza / Q000254:growth & development / Q000378:metabolism / Q000382:microbiology*; D010935:Plant Diseases / Q000382:microbiology; D000072417:Protein Domains; D016415:Sequence Alignment; D020798:Two-Hybrid System Techniques
chemical_list: D000071183:Autophagy-Related Proteins; D005656:Fungal Proteins; D006003:Glycogen