title: Assessment of genotoxic and antigenotoxic activities of artepillin C in somatic cells of Drosophila melanogaster.
abstract: The significant contents of artepillin C (AC) in green propolis have prompted research on the biological activities of the compound. The present study evaluated the activity of this phenolic compound on DNA, assessing its genotoxic and antigenotoxic potentials in the somatic mutation and recombination test in Drosophila melanogaster. The standard (ST) and high-bioactivation (HB) crosses were used in the assessment of genotoxic potential, since they express cytochrome P450 metabolization enzymes differently. In the 0.1-1.6 mM concentration range, AC did not have any genotoxic action in either cross. Antigenotoxic potential was investigated using the ST cross. In co- and post-treatment protocols, AC 0.4, 0.8, and 1.6 mM did not modulate mutagenic action of ethyl methanesulphonate. However, though it did not influence the frequency of damage induced by mitomycin C in co-treatment, AC reduced genotoxicity of the mutagen when administered after damage, but only at 0.4 mM. This modulation is associated with the reduction of genetic damage caused by recombinational events. The results of the present study and literature findings indicate that the various responses elicited by AC, namely induction of DNA damage, production of genetic lesions, or activation of DNA repair mechanisms are functions of AC concentration.
authors.affiliation: PPG em Biologia Celular e Molecular Aplicada à Saúde (PPGBIOSAÚDE), Laboratório de Toxicidade Genética (TOXIGEN), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil.
authors.forename: Carmem Regine Faleiro
authors.initials: CRF
authors.lastname: Rodrigues
authors.affiliation: PPG em Biologia Celular e Molecular Aplicada à Saúde (PPGBIOSAÚDE), Laboratório de Toxicidade Genética (TOXIGEN), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil.
authors.forename: Luciana Ciarelli
authors.initials: LC
authors.lastname: Plentz
authors.affiliation: PPG em Biologia Celular e Molecular Aplicada à Saúde (PPGBIOSAÚDE), Laboratório de Toxicidade Genética (TOXIGEN), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil.
authors.forename: Mariana do Amaral
authors.initials: MDA
authors.lastname: Flores
authors.affiliation: PPG em Biologia Celular e Molecular Aplicada à Saúde (PPGBIOSAÚDE), Laboratório de Toxicidade Genética (TOXIGEN), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil.
authors.forename: Rafael Rodrigues
authors.initials: RR
authors.lastname: Dihl
authors.affiliation: PPG em Biologia Celular e Molecular Aplicada à Saúde (PPGBIOSAÚDE), Laboratório de Toxicidade Genética (TOXIGEN), Universidade Luterana do Brasil, ULBRA, Canoas, RS, Brazil. Electronic address: mauriciol@ulbra.br.
authors.forename: Mauricio
authors.initials: M
authors.lastname: Lehmann
mesh_terms: D000818:Animals; D002478:Cells, Cultured; D004249:DNA Damage / Q000187:drug effects* / Q000235:genetics; D004331:Drosophila melanogaster / Q000187:drug effects* / Q000235:genetics; D009152:Mutagenicity Tests / Q000379:methods; D019384:Nucleic Acid Synthesis Inhibitors / Q000633:toxicity; D010666:Phenylpropionates / Q000633:toxicity*; D011995:Recombination, Genetic / Q000235:genetics*
chemical_list: D019384:Nucleic Acid Synthesis Inhibitors; D010666:Phenylpropionates; C102259:artepillin C
keywords: DNA damage; Mutation; Propolis; SMART assay; Somatic recombination