title: Redox proteomic identification of carbonylated proteins in autism plasma: insight into oxidative stress and its related biomarkers in autism.
abstract: BACKGROUND
  Autism is a severe childhood neurological disorder with poorly understood etiology and pathology. Currently, there is no authentic laboratory test to confirm the diagnosis of autism. Oxidative damage may play a central role in the pathogenesis of autism. Present study is an effort to search for possible biomarkers of autism and further clarify the molecular changes associated with oxidative stress that occurs in the plasma of autistic children.
  
  
  METHODS
  We performed redox proteomics analysis to compare carbonylated proteins in the plasma of autistic subjects and healthy controls. Immunoprecipitation and Western blot analysis were used to validate carbonylated proteins identified by the redox proteomics.
  
  
  RESULTS
  Protein carbonylation levels in two proteins, complement component C8 alpha chain and Ig kappa chain C were found to be significantly increased in autistic patients compared with controls. These two proteins were successfully validated via immunoprecipitation and Western blot analysis.
  
  
  CONCLUSIONS
  The results further highlight the role of oxidative stress in the pathogenesis of autism and provide some information for the diagnosis and/or monitoring of autism.
authors.affiliation: Early Childhood Development Center, Populations and Family Planning Hospital of Baoan, Shenzhen, 518101 People's Republic of China.
authors.forename: Chengyun
authors.initials: C
authors.lastname: Feng
authors.affiliation: College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 People's Republic of China.
authors.forename: Youjiao
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Early Childhood Development Center, Populations and Family Planning Hospital of Baoan, Shenzhen, 518101 People's Republic of China.
authors.forename: Jintao
authors.initials: J
authors.lastname: Pan
authors.affiliation: College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 People's Republic of China.
authors.forename: Aochu
authors.initials: A
authors.lastname: Yang
authors.affiliation: Early Childhood Development Center, Populations and Family Planning Hospital of Baoan, Shenzhen, 518101 People's Republic of China.
authors.forename: Li
authors.initials: L
authors.lastname: Niu
authors.affiliation: Early Childhood Development Center, Populations and Family Planning Hospital of Baoan, Shenzhen, 518101 People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Min
authors.affiliation: Early Childhood Development Center, Populations and Family Planning Hospital of Baoan, Shenzhen, 518101 People's Republic of China.
authors.forename: Xianling
authors.initials: X
authors.lastname: Meng
authors.affiliation: College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 People's Republic of China.
authors.forename: Liping
authors.initials: L
authors.lastname: Liao
authors.affiliation: College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 People's Republic of China.
authors.forename: Kaoyuan
authors.initials: K
authors.lastname: Zhang
authors.affiliation: College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060 People's Republic of China.
authors.forename: Liming
authors.initials: L
authors.lastname: Shen
keywords: Autism; Biomarkers; Plasma; Protein carbonylation; Redox proteomics