title: The role of bradykinin in lung ischemia-reperfusion injury in a rat lung transplantation model.
abstract: To investigate the role of bradykinin in a rat lung transplantation (LTx) model and preliminarily discuss the relationship between bradykinin and CD26/DPP-4.
  Rats were randomly divided into four groups: Control (CON), Sham, low potassium dextranglucose (LPD), and AB192 (n=15/group). Orthotopic single LTx was performed in the LPD and AB192 groups. The donor lungs were flush-perfused and preserved with low potassium dextranglucose (LPD) or LPD+CD26/DPP-4 catalytic inhibitor (AB192). LTx was performed after 18 h cold ischemia time and harvested two days post-LTx. Blood gas analysis (PO2), wet/dry weight ratio (W/D), myeloperoxidase activity (MPO), and lipid peroxidation (MDA) were analyzed at 48 hr after transplantation. Immunohistochemical (IHC) analysis was performed in the same sample and validated by Western-Blot.
  Compared to the LPD group, the AB192 group showed higher PO2, lower W/D ratio, and decreased MPO and MDA. IHC studies showed strong bradykinin β2 receptor (B2R) staining in the LPD group, especially in inflammatory cells, alveolar macrophages, and respiratory epithelial cells. Expression of B2R by Western-Blot was significantly different between the AB192 and LPD groups.
  Bradykinin may be a competitive substrate of DPP-4, and decreased bradykinin levels may enhance protective effects against ischemia/reperfusion injury during LTx.
authors.affiliation: PhD, Department of Cardiovascular Surgery, Renmin Hospital, Wuhan University, China. Conception and design of the study, acquisition and interpretation of data, manuscript writing.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Tang
authors.affiliation: PhD, Full Professor, Department of Cardiovascular Surgery, Renmin Hospital, Wuhan University, China. Design and supervised all phases of the study, critical revision.
authors.forename: Zhiwei
authors.initials: Z
authors.lastname: Wang
authors.affiliation: PhD, Department of Cardiovascular Surgery, Renmin Hospital, Wuhan University, China. Acquisition of data, critical revision.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Hu
authors.affiliation: PhD, Department of Cardiovascular Surgery, Renmin Hospital, Wuhan University, China. Acquisition of data, critical revision.
authors.forename: Min
authors.initials: M
authors.lastname: Zhang
authors.affiliation: PhD, Department of Cardiovascular Surgery, Renmin Hospital, Wuhan University, China. Acquisition of data, critical revision.
authors.forename: Luocheng
authors.initials: L
authors.lastname: Li
authors.affiliation: Master, Department of Cardiovascular Surgery, Renmin Hospital, Wuhan University, China. Acquisition of data.
authors.forename: Bowen
authors.initials: B
authors.lastname: Li
mesh_terms: D000818:Animals; D015153:Blotting, Western; D001920:Bradykinin / Q000502:physiology*; D065094:Bradykinin B2 Receptor Antagonists / Q000378:metabolism; D018819:Dipeptidyl Peptidase 4 / Q000502:physiology*; D004195:Disease Models, Animal; D007150:Immunohistochemistry; D015227:Lipid Peroxidation; D008168:Lung / Q000098:blood supply* / Q000187:drug effects; D016040:Lung Transplantation; D008297:Male; D055031:Primary Graft Dysfunction / Q000473:pathology* / Q000503:physiopathology; D011897:Random Allocation; D051381:Rats; D015427:Reperfusion Injury / Q000378:metabolism / Q000473:pathology* / Q000503:physiopathology
chemical_list: D065094:Bradykinin B2 Receptor Antagonists; C042807:DPP4 protein, human; D018819:Dipeptidyl Peptidase 4; D001920:Bradykinin