title: pH sensitive dexamethasone encapsulated laponite nanoplatelets: Release mechanism and cytotoxicity.
abstract: The purpose of this study was to develop an efficient strategy to use laponite (LAP) nanoplates as a platform for the efficient release of anionic dexamethasone (DEX). Results revealed that DEX was encapsulated into the interlayer space of LAP nanodisks through an intercalation process with a high loading efficiency of 95.10±0.80%. X-Ray diffraction (XRD) patterns and Fourier transform infrared (FTIR) spectra of the hybrid LAP/DEX nanoplates (LD-NPs) indicated that DEX molecules could successfully adsorb into the LAP nanoplates depending on the pH value. Moreover, in vitro drug release study showed that the release of DEX from LD-NPs was pH-dependent, and DEX released at a faster rate at acidic pH (pH=5.4) than physiological one. Importantly, the results of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay confirmed that the released DEX from LD-NPs not only did not show cytotoxic effect but also improved the viability of MG63 cells compared to LAP-free samples (DEX enriched medium). Our work indicated that LAP nanoplates could be a promising candidate for release of anionic DEX in the controlled manner depending on the pH environment. Moreover, the merits of LD-NPs such as good cytocompatibility, excellent physiological stability and sustained pH-responsive release properties, make them a promising platform for the delivery of other therapeutic agents beyond DEX.
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: M
authors.initials: M
authors.lastname: Roozbahani
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran. Electronic address: ma.kharaziha@gmail.com.
authors.forename: M
authors.initials: M
authors.lastname: Kharaziha
authors.affiliation: Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
authors.forename: R
authors.initials: R
authors.lastname: Emadi
mesh_terms: D045744:Cell Line, Tumor; D002470:Cell Survival / Q000187:drug effects; D003907:Dexamethasone / Q000008:administration & dosage* / Q000737:chemistry*; D065546:Drug Liberation; D006801:Humans; D006863:Hydrogen-Ion Concentration; D049329:Nanostructures / Q000008:administration & dosage* / Q000737:chemistry*; D017640:Silicates / Q000008:administration & dosage* / Q000737:chemistry*
chemical_list: D017640:Silicates; D003907:Dexamethasone; C524813:laponite
keywords: Cytotoxicity; Dexamethasone; Drug release; Laponite nanoplates