title: Isolation and Characterization of Aortic Dendritic Cells and Lymphocytes in Atherosclerosis.
abstract: Dendritic cells (DCs) are central to initiate antigen-specific immunity and tolerance. The in vivo development and distribution of DCs are now better understood even in nonlymphoid tissues [1]. Atherosclerosis is a chronic inflammatory disease of blood vessels and DCs are highly enriched in the intimal area of the aorta, which is predisposed to develop atherosclerosis. Previously, we were the first to show antigen presenting DCs and their subsets in the aorta [2, 3]. Here, we discuss several useful methods to characterize not only DCs but also other immune cells in steady state and atherosclerotic aorta. These comprise multiparameter flow cytometry strategies including intracellular staining and cell sorting, en face immunohistochemistry of DCs and regulatory T cells (Tregs), and Oil Red O staining of atherosclerotic lesions in the aorta.
authors.affiliation: Laboratory of Cellular Physiology and Immunology, Institut de Recherches Cliniques de Montréal, 110 Ave des pins Quest, Montréal, QC, Canada, H2 W 1R7.|Division of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC, Canada.
authors.forename: Tae Jin
authors.initials: TJ
authors.lastname: Yun
authors.affiliation: Laboratory of Cellular Physiology and Immunology, Institut de Recherches Cliniques de Montréal, 110 Ave des pins Quest, Montréal, QC, Canada, H2 W 1R7.|Department of Microbiology, Infectiology, and Immunology, University of Montreal, Montreal, QC, Canada.
authors.forename: Jun Seong
authors.initials: JS
authors.lastname: Lee
authors.affiliation: Department of Life Science, College of Natural Sciences, Research Institute of Natural Sciences, Hanyang University, Seoul, Republic of Korea.
authors.forename: Dahee
authors.initials: D
authors.lastname: Shim
authors.affiliation: Department of Life Science, College of Natural Sciences, Research Institute of Natural Sciences, Hanyang University, Seoul, Republic of Korea. jchoi75@hanyang.ac.kr.
authors.forename: Jae-Hoon
authors.initials: JH
authors.lastname: Choi
authors.affiliation: Laboratory of Cellular Physiology and Immunology, Institut de Recherches Cliniques de Montréal, 110 Ave des pins Quest, Montréal, QC, Canada, H2 W 1R7. Cheolho.Cheong@ircm.qc.ca.|Division of Experimental Medicine, Department of Medicine, McGill University, Montreal, QC, Canada. Cheolho.Cheong@ircm.qc.ca.|Department of Microbiology, Infectiology, and Immunology, University of Montreal, Montreal, QC, Canada. Cheolho.Cheong@ircm.qc.ca.
authors.forename: Cheolho
authors.initials: C
authors.lastname: Cheong
mesh_terms: D000818:Animals; D015703:Antigens, CD / Q000235:genetics / Q000276:immunology; D001011:Aorta / Q000276:immunology / Q000473:pathology*; D001057:Apolipoproteins E / Q000172:deficiency / Q000235:genetics; D050197:Atherosclerosis / Q000235:genetics / Q000276:immunology / Q000473:pathology*; D001391:Azo Compounds; D015415:Biomarkers / Q000378:metabolism; D003713:Dendritic Cells / Q000276:immunology / Q000473:pathology*; D005434:Flow Cytometry / Q000379:methods*; D051858:Forkhead Transcription Factors / Q000235:genetics / Q000276:immunology; D015870:Gene Expression; D007150:Immunohistochemistry / Q000379:methods; D051379:Mice; D008810:Mice, Inbred C57BL; D018345:Mice, Knockout; D008867:Microtomy / Q000379:methods; D011973:Receptors, LDL / Q000172:deficiency / Q000235:genetics; D050378:T-Lymphocytes, Regulatory / Q000276:immunology / Q000473:pathology*; D017539:Tunica Intima / Q000276:immunology / Q000473:pathology*
chemical_list: D015703:Antigens, CD; D001057:Apolipoproteins E; D001391:Azo Compounds; D015415:Biomarkers; D051858:Forkhead Transcription Factors; C498833:Foxp3 protein, mouse; D011973:Receptors, LDL; C011049:oil red O
keywords: Aorta; Atherosclerosis; CD11c; Dendritic cells; Macrophage; Regulatory T cells (Tregs)