title: Molecular identification of Mycobacterium tuberculosis in cattle.
abstract: Bovine tuberculosis continued to be a re-emerging problem in some countries especially in endemic areas due to the fact that human and animal health surveillance system is not adopted to diagnose the infection. This crisis can be attributed due to sharing of the same habitat especially in rural areas. In the present study, a total of 148 samples were collected from cattle for isolation over a period of 3 years from cattle with and without lesions, of which 67 isolates were obtained by culture. Fifty one isolates were identified as Mycobacterium tuberculosis complex (MTBC) by IS6110 PCR of which 43 (84.3%) were identified as M. tuberculosis and 08 (15.6%) were identified as M. bovis by using 12.7kb fragment multiplex PCR. Among this, 31 isolates which were positive for IS6110 PCR were subjected to spoligotyping and revealed 28 isolates belonging to MANU1 strain of M. tuberculosis. This study clearly indicates that high prevalence of M. tuberculosis than M. bovis in bovine was identified by means of culture and by molecular methods M. tuberculosis can affect cattle producing lesion in contradiction to the earlier thoughts. This study speculates that M. tuberculosis MANU1 strain infection in cattle could be due to spill over from human or other non specific hosts in tuberculosis endemic areas. Though bovine tuberculosis due to M. tuberculosis in cattle is not considered a serious threat worldwide, in countries where human TB is endemic, M. tuberculosis infection of cattle needs to be considered.
authors.affiliation: Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai-600 051, Tamilnadu, India. Electronic address: sweetvet06@gmail.com.
authors.forename: N
authors.initials: N
authors.lastname: Sweetline Anne
authors.affiliation: Veterinary College and Research Institute, Orathanadu, TANUVAS, Tamilnadu, India. Electronic address: romasa68@yahoo.com.
authors.forename: B S M
authors.initials: BS
authors.lastname: Ronald
authors.affiliation: Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai-600 051, Tamilnadu, India.
authors.forename: T M A Senthil
authors.initials: TM
authors.lastname: Kumar
authors.affiliation: National Institute for Research In Tuberculosis (NIRT), Chennai, Tamilnadu, India.
authors.forename: P
authors.initials: P
authors.lastname: Kannan
authors.affiliation: Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University (TANUVAS), Chennai-600 051, Tamilnadu, India.
authors.forename: A
authors.initials: A
authors.lastname: Thangavelu
mesh_terms: D000818:Animals; D002417:Cattle; D004269:DNA, Bacterial / Q000032:analysis; D005838:Genotype; D006801:Humans; D007194:India / Q000453:epidemiology; D058889:Molecular Typing; D060885:Multiplex Polymerase Chain Reaction; D009163:Mycobacterium bovis / Q000235:genetics / Q000302:isolation & purification; D009169:Mycobacterium tuberculosis / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification; D015995:Prevalence; D014380:Tuberculosis, Bovine / Q000175:diagnosis / Q000453:epidemiology / Q000382:microbiology* / Q000473:pathology; D015047:Zoonoses / Q000453:epidemiology / Q000382:microbiology
chemical_list: D004269:DNA, Bacterial
keywords: Bovine tuberculosis; Isolation and identification; Multiplex PCR; Spoligotyping