title: Genomewide association studies for hematological traits and T lymphocyte subpopulations in a Duroc × Erhualian F resource population.
abstract: It has been shown that hematological traits can act as important indicators of immune function in both humans and livestock. T lymphocytes are key components of the adaptive immune system, playing a critical role in immune response. To identify genomic regions affecting hematological traits and T lymphocyte subpopulations, we performed both a SNP-based genomewide association study (GWAS) and a haplotype analysis for 20 hematological traits and 8 T cell subpopulations at 3 different time points (d 20, 33, and 35) in a Duroc × Erhualian F intercross population. Bonferroni correction was used to calculate the threshold -values for suggestive and 5% genomewide significance levels. In total, for SNP-based GWAS, we detected 96 significant SNP, including 15 genomewide-significant SNP, associated with 23 hematological traits and 234 significant SNP, including 27 genomewide-significant SNP, associated with 8 T cell subpopulations. Meanwhile, we identified 563 significant SNP, including 7 genomewide-significant SNP, associated with 5 hematological traits and 2,407 significant SNP, including 1,261 genomewide-significant SNP, associated with 8 T cell subpopulations by haplotype analysis. Among the significant regions detected, we propose both the () gene and the () gene on SSC3 as plausible candidate genes associated with CD/CD T lymphocytes at d 20. The findings provide insights into the basis of molecular mechanisms that are involved with immune response in the domestic pig and would aid further identification of causative mutations.
authors.forename: J
authors.initials: J
authors.lastname: Zhang
authors.forename: J H
authors.initials: JH
authors.lastname: Chen
authors.forename: X D
authors.initials: XD
authors.lastname: Liu
authors.forename: H Y
authors.initials: HY
authors.lastname: Wang
authors.forename: X L
authors.initials: XL
authors.lastname: Liu
authors.forename: X Y
authors.initials: XY
authors.lastname: Li
authors.forename: Z F
authors.initials: ZF
authors.lastname: Wu
authors.forename: M J
authors.initials: MJ
authors.lastname: Zhu
authors.forename: S H
authors.initials: SH
authors.lastname: Zhao
mesh_terms: D000818:Animals; D005260:Female; D055106:Genome-Wide Association Study; D023281:Genomics; D006239:Haplotypes / Q000235:genetics; D006405:Hematology; D016131:Lymphocyte Subsets; D008297:Male; D010641:Phenotype; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D040641:Quantitative Trait Loci / Q000235:genetics; D034421:Sus scrofa / Q000235:genetics; D013552:Swine / Q000235:genetics* / Q000502:physiology