title: SlipChip Device for Digital Nucleic Acid Amplification.
abstract: Digital nucleic acid amplification (Digital NAA) quantifies nucleic acid by compartmentalizing a sample of DNA or RNA into a large number of discrete partitions and performing parallel nucleic acid amplification, such as polymerase chain reaction (PCR) or isothermal amplification reactions. With the counts of positive wells, total number of wells, and volumes of wells, the concentration of the target nucleic acid in the sample can be quantified. Digital NAA is considered increasingly powerful for ultra-sensitive detection and accurate quantification of nucleic acid for biological research and potentially medical diagnostics. Here, we describe glass SlipChip devices to perform digital NAA without cumbersome manual manipulation or complex fluidic control systems.
authors.affiliation: SlipChip Corporation, 230 Constitution Drive, Menlo Park, CA, 94025, USA. fengshen@slipchip.com.
authors.forename: Feng
authors.initials: F
authors.lastname: Shen
mesh_terms: D056656:Lab-On-A-Chip Devices; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation* / Q000379:methods; D044085:Microfluidics / Q000295:instrumentation* / Q000379:methods; D008856:Microscopy, Fluorescence; D021141:Nucleic Acid Amplification Techniques / Q000295:instrumentation* / Q000379:methods; D061848:Optical Imaging / Q000295:instrumentation / Q000379:methods; D016133:Polymerase Chain Reaction; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D013223:Statistics as Topic / Q000379:methods
keywords: Digital PCR; Lab on a chip; Microfluidics; Nucleic acid amplification; Quantification; Single molecule; Ultra-sensitive detection