title: Optical coherence tomography-based topography determination of corneal grafts in eye bank cultivation.
abstract: Vision loss due to corneal injuries or diseases can be treated by transplantation of human corneal grafts (keratoplasty). However, quality assurance in retrieving and cultivating the tissue transplants is confined to visual and microbiological testing. To identify previous refractive surgery or morphological alterations, an automatic, noncontact, sterile screening procedure is required. Twenty-three corneal grafts have been measured in organ culture with a clinical spectral-domain optical coherence tomographer. Employing a biconic surface fit with 10 degrees of freedom, the radii of curvature and conic constants could be estimated for the anterior and posterior corneal surfaces. Thereupon, central corneal thickness, refractive values, and astigmatism have been calculated. Clinical investigations are required to elaborate specific donor–host matching in the future.
authors.affiliation: Saarland University, Institute of Experimental Ophthalmology, Kirrberger Street 100, Building 22, 66421 Homburg/Saar, Germany.
authors.forename: Angela
authors.initials: A
authors.lastname: Damian
authors.affiliation: Saarland University Medical Center (UKS), Department of Ophthalmology, Kirrberger Street 100, Building 22, 66421 Homburg/Saar, Germany.
authors.forename: Berthold
authors.initials: B
authors.lastname: Seitz
authors.affiliation: Saarland University, Institute of Experimental Ophthalmology, Kirrberger Street 100, Building 22, 66421 Homburg/Saar, Germany.
authors.forename: Achim
authors.initials: A
authors.lastname: Langenbucher
authors.affiliation: Saarland University, Institute of Experimental Ophthalmology, Kirrberger Street 100, Building 22, 66421 Homburg/Saar, Germany.
authors.forename: Timo
authors.initials: T
authors.lastname: Eppig
mesh_terms: D003315:Cornea; D019781:Corneal Topography / Q000379:methods*; D016039:Corneal Transplantation; D005125:Eye Banks; D006801:Humans; D041623:Tomography, Optical Coherence