title: Antioxidant Effect of Natural Table Olives Phenolic Extract Against Oxidative Stress and Membrane Damage in Enterocyte-Like Cells.
abstract: The phenolic fraction of a naturally fermented cultivar of table olives, "Tonda di Cagliari," was investigated for the ability to protect Caco-2 cells against oxidative stress and membrane damage induced by tert-butyl hydroperoxyde (TBH). TBH exposure resulted in an alteration of cellular redox status, with an increase in reactive oxygen species (ROS) and a decrease in reduced glutathione (GSH) level. A loss of the epithelial integrity, as indicated by the decrease of the transepithelial electrical resistance value, was also observed over time, together with an intense lipid peroxidation process. The olives phenolic extract significantly counteracted ROS generation and subsequent alteration of monolayer integrity and membrane oxidative damage. The protective action of the extract is likely due to the scavenging ability of its main components, as hydroxytyrosol, oleuropein, and verbascoside among the secoiridoids and derivatives. Since olives phenolic compounds concentrate in the intestinal lumen, they may be a useful tool in the prevention of intestinal disorders related to oxidative damage.
authors.affiliation: Dept. of Biomedical Sciences, Unit of Experimental Pathology, Univ. of Cagliari, Cittadella Universitaria, SS 554, Monserrato (CA), 09042, Italy.
authors.forename: Gabriele
authors.initials: G
authors.lastname: Serreli
authors.affiliation: Dept. of Biomedical Sciences, Unit of Experimental Pathology, Univ. of Cagliari, Cittadella Universitaria, SS 554, Monserrato (CA), 09042, Italy.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Incani
authors.affiliation: Dept. of Biomedical Sciences, Unit of Experimental Pathology, Univ. of Cagliari, Cittadella Universitaria, SS 554, Monserrato (CA), 09042, Italy.
authors.forename: Angela
authors.initials: A
authors.lastname: Atzeri
authors.affiliation: Dept. of Life and Environmental Sciences, Univ. of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy.
authors.forename: Alberto
authors.initials: A
authors.lastname: Angioni
authors.affiliation: Agricultural Research Agency of Sardinia (Agris Sardegna), Km 18.600 S.S. 291, Loc. Bonassai, Sassari, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Campus
authors.affiliation: Agricultural Research Agency of Sardinia (Agris Sardegna), Km 18.600 S.S. 291, Loc. Bonassai, Sassari, Italy.
authors.forename: Emanuele
authors.initials: E
authors.lastname: Cauli
authors.affiliation: Agricultural Research Agency of Sardinia (Agris Sardegna), Km 18.600 S.S. 291, Loc. Bonassai, Sassari, Italy.
authors.forename: Roberto
authors.initials: R
authors.lastname: Zurru
authors.affiliation: Dept. of Biomedical Sciences, Unit of Experimental Pathology, Univ. of Cagliari, Cittadella Universitaria, SS 554, Monserrato (CA), 09042, Italy.
authors.forename: Monica
authors.initials: M
authors.lastname: Deiana
mesh_terms: D000975:Antioxidants / Q000494:pharmacology*; D018938:Caco-2 Cells; D002462:Cell Membrane / Q000187:drug effects / Q000378:metabolism; D020895:Enterocytes / Q000187:drug effects* / Q000378:metabolism; D005978:Glutathione / Q000378:metabolism; D006801:Humans; D015227:Lipid Peroxidation / Q000187:drug effects; D031658:Olea / Q000737:chemistry*; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000187:drug effects*; D010636:Phenols / Q000494:pharmacology*; D010936:Plant Extracts / Q000494:pharmacology*; D017382:Reactive Oxygen Species
chemical_list: D000975:Antioxidants; D010636:Phenols; D010936:Plant Extracts; D017382:Reactive Oxygen Species; D005978:Glutathione
keywords: Caco-2 cells; Tonda di Cagliari; natural table olives; oxidative stress; phenols