title: Alleviation Mechanisms of Selenium on Cadmium-Spiked Neutrophil Injury to Chicken.
abstract: To determine the negative effects of cadmium (Cd) exposure and the protective role of selenium (Se) on Cd-spiked neutrophils of chicken, forty-eight 28-day-old Isa Brown male chickens were divided randomly into four groups. Group I (control group) was fed with the basic diet containing 0.2 mg/kg Se. Group II (Se-treated group) was fed with the basic diet supplemented with Na2SeO3, and the total Se content was 2 mg/kg. Group III (Se/Cd-treated group) was fed with the basic diet supplemented with Na2SeO3; the total Se content was 2 mg/kg and supplemented with 150 mg/kg CdCl2. Group IV (Cd-treated group) was fed with the basic diet supplemented with 150 mg/kg CdCl2. Analyses of inflammatory factors, cytokines, and heat shock protein (Hsp) messenger RNA (mRNA) expression were detected by real-time PCR (RT-PCR). Additionally, we evaluated the phagocytic rate of neutrophils in peripheral blood. First, we observed that Cd significantly induced the mRNA expression levels of inflammatory factors NF-κB, iNOS, COX-2, and TNF-α, while Se/Cd treatment reduced their mRNA expression, although these expression levels remained higher than that of the control group. In addition, the mRNA expression levels of cytokines (IL-2, IL-4, and IL-10) for the Se-treated group exhibited significant differences between the Se/Cd-treated group and the Cd-treated group. Furthermore, the mRNA expression levels of Hsps demonstrated that the Se/Cd-treated group and the Cd-treated group were significantly higher (P < 0.05) than the control group and the Se-treated group. These results demonstrated that Se presented partial protection on Cd-spiked neutrophils of chicken with Hsps being involved in the process of the Cd-spiked toxic effects in chicken peripheral blood neutrophils.
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Siran
authors.initials: S
authors.lastname: Tan
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Qianru
authors.initials: Q
authors.lastname: Chi
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Tianqi
authors.initials: T
authors.lastname: Liu
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Zhepeng
authors.initials: Z
authors.lastname: Sun
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China.
authors.forename: Yahong
authors.initials: Y
authors.lastname: Min
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. zhangziwei@neau.edu.cn.
authors.forename: Ziwei
authors.initials: Z
authors.lastname: Zhang
authors.affiliation: College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People's Republic of China. lishu@neau.edu.cn.
authors.forename: Shu
authors.initials: S
authors.lastname: Li
mesh_terms: D000821:Animal Feed; D000818:Animals; D030161:Avian Proteins / Q000096:biosynthesis; D002104:Cadmium / Q000494:pharmacology; D019256:Cadmium Chloride / Q000494:pharmacology*; D002645:Chickens / Q000378:metabolism*; D005786:Gene Expression Regulation / Q000187:drug effects; D008297:Male; D009504:Neutrophils / Q000378:metabolism*; D010587:Phagocytosis / Q000187:drug effects*; D012643:Selenium / Q000494:pharmacology; D018038:Sodium Selenite / Q000494:pharmacology*
chemical_list: D030161:Avian Proteins; D002104:Cadmium; D012643:Selenium; D018038:Sodium Selenite; D019256:Cadmium Chloride
keywords: Cadmium; Chicken; Cytokines; Heat shock protein; Neutrophils; Selenium