title: Development of Advanced Dressings for the Delivery of Progenitor Cells.
abstract: Culture surfaces that substantially reduce the degree of cell manipulation in the delivery of cell sheets to patients are described. These surfaces support the attachment, culture, and delivery of multipotent adult progenitor cells (MAPC). It was essential that the processes of attachment/detachment to the surface did not affect cell phenotype nor the function of the cultured cells. Both acid-based and amine-based surface coatings were generated from acrylic acid, propanoic acid, diaminopropane, and heptylamine precursors, respectively. While both functional groups supported cell attachment/detachment, amine coated surfaces gave optimal performance. X-ray photoelectron spectroscopy (XPS) showed that at a primary amine to carbon surface ratio of between 0.01 and 0.02, greater than 90% of attached cells were effectively transferred to a model wound bed. A dependence on primary amine concentration has not previously been reported. After 48 h of culture on the optimized amine surface, PCR, functional, and viability assays showed that MAPC retained their stem cell phenotype, full metabolic activity, and biological function. Consequently, in a proof of concept experiment, it was shown that this amine surface when coated onto a surgical dressing provides an effective and simple technology for the delivery of MAPC to murine dorsal excisional wounds, with MAPC delivery verified histologically. By optimizing for cell delivery using a combination of in vitro and in vivo techniques, we developed an effective surface for the delivery of MAPC in a clinically relevant format.
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Giles T S
authors.identifier: http://orcid.org/0000-0003-4557-9936
authors.initials: GT
authors.lastname: Kirby
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Stuart J
authors.initials: SJ
authors.lastname: Mills
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|ReGenesys BVBA, Bio-Incubator Leuven , Gaston Geenslaan 1, 3001 Heverlee, Belgium.
authors.forename: Liesbeth
authors.initials: L
authors.lastname: Vandenpoel
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|ReGenesys BVBA, Bio-Incubator Leuven , Gaston Geenslaan 1, 3001 Heverlee, Belgium.
authors.forename: Jef
authors.initials: J
authors.lastname: Pinxteren
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|Athersys, Inc. , Cleveland, Ohio 44115-2634, United States.
authors.forename: Anthony
authors.initials: A
authors.lastname: Ting
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Robert D
authors.initials: RD
authors.lastname: Short
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Allison J
authors.initials: AJ
authors.lastname: Cowin
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.|School of Engineering, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Andrew
authors.identifier: http://orcid.org/0000-0003-3215-8841
authors.initials: A
authors.lastname: Michelmore
authors.affiliation: Cooperative Research Centre for Cell Therapy Manufacturing , North Terrace, Adelaide, South Australia 5000, Australia.|Future Industries Institute, University of South Australia , Mawson Lakes, South Australia 5095, Australia.
authors.forename: Louise E
authors.identifier: http://orcid.org/0000-0003-0659-0884
authors.initials: LE
authors.lastname: Smith
mesh_terms: D053687:Adult Stem Cells; D000818:Animals; D001458:Bandages; D002478:Cells, Cultured; D006801:Humans; D051379:Mice; D039902:Multipotent Stem Cells; D013234:Stem Cells
keywords: cell delivery; cell therapy; chronic wounds; progenitor cell; surface modification