title: MicroRNA expression patterns in post-natal mouse skeletal muscle development.
abstract: MiRNAs are essential regulators of skeletal muscle development and homeostasis. To date, the role and regulation of miRNAs in myogenesis have been mostly studied in tissue culture and during embryogenesis. However, little information relating to miRNA regulation during early post-natal skeletal muscle growth in mammals is available. Using a high-throughput miRNA qPCR-based array, followed by stringent statistical and bioinformatics analysis, we describe the expression pattern and putative role of 768 miRNAs in the quadriceps muscle of mice aged 2 days, 2 weeks, 4 weeks and 12 weeks.
  
  
  
  Forty-six percent of all measured miRNAs were expressed in mouse quadriceps muscle during the first 12 weeks of life. We report unprecedented changes in miRNA expression levels over time. The expression of a majority of miRNAs significantly decreased with post-natal muscle maturation in vivo. MiRNA clustering identified 2 subsets of miRNAs that are potentially involved in cell proliferation and differentiation, mainly via the regulation of non-muscle specific targets.
  
  
  
  Collective miRNA expression in mouse quadriceps muscle is subjected to substantial levels of regulation during the first 12 weeks of age. This study identified a new suite of highly conserved miRNAs that are predicted to influence early muscle development. As such it provides novel knowledge pertaining to post-natal myogenesis and muscle regeneration in mammals.
authors.affiliation: Deakin University, School of Exercise and Nutrition Sciences, Institute for Physical Activity and Nutrition (I-PAN), Geelong, Australia. severine.lamon@deakin.edu.au.
authors.forename: Séverine
authors.initials: S
authors.lastname: Lamon
authors.affiliation: Deakin University, School of Exercise and Nutrition Sciences, Institute for Physical Activity and Nutrition (I-PAN), Geelong, Australia.
authors.forename: Evelyn
authors.initials: E
authors.lastname: Zacharewicz
authors.affiliation: The University of Western Australia, School of Anatomy, Physiology and Human Biology, Perth, WA, Australia.
authors.forename: Lauren C
authors.initials: LC
authors.lastname: Butchart
authors.affiliation: Deakin University, Biostatistics Unit, Faculty of Health, Geelong, Australia.
authors.forename: Liliana
authors.initials: L
authors.lastname: Orellana
authors.affiliation: Deakin University, School of Exercise and Nutrition Sciences, Institute for Physical Activity and Nutrition (I-PAN), Geelong, Australia.
authors.forename: Jasmine
authors.initials: J
authors.lastname: Mikovic
authors.affiliation: The University of Western Australia, School of Anatomy, Physiology and Human Biology, Perth, WA, Australia.
authors.forename: Miranda D
authors.initials: MD
authors.lastname: Grounds
authors.affiliation: Deakin University, School of Exercise and Nutrition Sciences, Institute for Physical Activity and Nutrition (I-PAN), Geelong, Australia.
authors.forename: Aaron P
authors.initials: AP
authors.lastname: Russell
mesh_terms: D000818:Animals; D020869:Gene Expression Profiling; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D035683:MicroRNAs / Q000235:genetics*; D024510:Muscle Development / Q000235:genetics*; D018482:Muscle, Skeletal / Q000254:growth & development*; D018006:Myogenic Regulatory Factors / Q000235:genetics
chemical_list: D035683:MicroRNAs; D018006:Myogenic Regulatory Factors
keywords: Cell differentiation; Cell proliferation; Growth; MiRNAs; Myogenesis; Skeletal muscle