title: Nanotopography Induced Human Bone MarrowMesangiogenic Progenitor Cells(MPCs) toMesenchymal Stromal Cells(MSCs) Transition.
abstract: Mesangiogenic progenitor cells(MPCs) are a very peculiar population of cells present in the human adult bone marrow, only recently discovered and characterized. Owing to their differentiation potential, MPCs can be considered progenitors for mesenchymal stromal cells (MSCs), and for this reason they potentially represent a promising cell population to apply for skeletal tissue regeneration applications. Here, we evaluate the effects of surface nanotopography on MPCs, considering the possibility that this specific physical stimulus alone can trigger MPC differentiation toward the mesenchymal lineage. In particular, we exploit nanogratings to deliver a mechanical, directional stimulus by contact interaction to promote cell morphological polarization and stretching. Following this interaction, we study the MPC-MSC transition by i. analyzing the change in cell morphotype by immunostaining of the key cell-adhesion structures and confocal fluorescence microscopy, and ii. quantifying the expression of cell-phenotype characterizing markers by flow cytometry. We demonstrate that the MPC mesengenic differentiation can be induced by the solely interaction with the NGs, in absence of any other external, chemical stimulus. This aspect is of particular interest in the case of multipotent progenitors as MPCs that, retaining both mesengenic and angiogenic potential, possess a high clinical appeal.
authors.affiliation: NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore Pisa, Italy.
authors.forename: Sara
authors.initials: S
authors.lastname: Antonini
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa Pisa, Italy.
authors.forename: Marina
authors.initials: M
authors.lastname: Montali
authors.affiliation: Dipartimento di Chimica, Materiali e Ingegneria Chimica "G.Natta", Politecnico di Milano Milan, Italy.
authors.forename: Emanuela
authors.initials: E
authors.lastname: Jacchetti
authors.affiliation: NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore Pisa, Italy.
authors.forename: Sandro
authors.initials: S
authors.lastname: Meucci
authors.affiliation: Department of Surgical, Medical and Molecular Pathology and Critical Care Medicine, University of Pisa Pisa, Italy.
authors.forename: Paolo D
authors.initials: PD
authors.lastname: Parchi
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa Pisa, Italy.
authors.forename: Serena
authors.initials: S
authors.lastname: Barachini
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa Pisa, Italy.
authors.forename: Francesca M
authors.initials: FM
authors.lastname: Panvini
authors.affiliation: Department of Clinical and Experimental Medicine, University of Pisa Pisa, Italy.
authors.forename: Simone
authors.initials: S
authors.lastname: Pacini
authors.affiliation: Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa Pisa, Italy.
authors.forename: Iacopo
authors.initials: I
authors.lastname: Petrini
authors.affiliation: NEST, Istituto Nanoscienze-CNR and Scuola Normale Superiore Pisa, Italy.
authors.forename: Marco
authors.initials: M
authors.lastname: Cecchini
keywords: bone marrow culture; mesangiogenic progenitor cells; mesenchymal stromal cells; nanograting; polyethylene terephthalate