title: A new method of genotyping MDX4CVmice by PCR-RFLP analysis.
abstract: The mdx4cvmouse is a common model to study Duchenne muscular dystrophy. The most used methodology to identify the genotype of these mice is Sanger DNA sequencing.
  
  
  
  Here, we provide a simple, cost-effective alternative approach to identify the wild-type, heterozygous, or homozygous/hemizygous genotypes of these mice, using commonly available laboratory equipment and reagents.
  
  
  
  Our technique exploits a restriction fragment length polymorphism that is generated by the point mutation found in exon 53 of mdx4cvmice.
  
  
  
  This technique can benefit laboratories that require complex breeding strategies involving mdx4cvmice. Muscle Nerve 56: 522-524, 2017.
authors.affiliation: Department of Orthopaedic Surgery, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania, USA.
authors.forename: Elisia D
authors.initials: ED
authors.lastname: Tichy
authors.affiliation: Department of Orthopaedic Surgery, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, Pennsylvania, USA.|Department of Cell and Developmental Biology, Perelman School of Medicine, The University of Pennsylvania, Philadelphia, PA, USA.
authors.forename: Foteini
authors.initials: F
authors.lastname: Mourkioti
mesh_terms: D000818:Animals; D005260:Female; D005838:Genotype; D008297:Male; D051379:Mice; D008810:Mice, Inbred C57BL; D018101:Mice, Inbred mdx; D020388:Muscular Dystrophy, Duchenne / Q000235:genetics*; D012150:Polymorphism, Restriction Fragment Length / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction / Q000379:methods*
keywords: DMD; Duchenne muscular dystrophy; PCR-RFLP; genotyping; mdx; mdx4cv; restriction fragment length polymorphism