title: Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis.
abstract: The pine weevil, Hylobius abietis, is a severe forest pest insect as it feeds on newly planted conifer seedlings. To identify and develop an antifeedant could be one step towards the protection of seedlings from feeding damage by the pine weevil. With the aim to trace the origin of the antifeedants previously found in feces of the pine weevil, we investigated the culturable bacteria associated with the gut and identified the volatiles they produced. Bacterial isolates were identified by 16S ribosomal RNA gene analysis. The volatile emissions of selected bacteria, cultivated on NB media or on the grated phloem of Scots pine twigs dispersed in water, were collected and analyzed by solid-phase microextraction gas chromatography-mass spectrometry. The bacterial isolates released a variety of compounds, among others 2-methoxyphenol, 2-phenylethanol, 3-methyl-1-butanol, 1-octen-3-ol, 3-octanone, dimethyl disulfide, and dimethyl trisulfide. A strong antifeedant effect was observed by 2-phenylethanol, which could thus be a good candidate for use to protect planted conifer seedlings against feeding damage caused by H. abietis.
authors.affiliation: Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Ecological Chemistry Group, SE-100 44, Stockholm, Sweden.|Royal Institute of Technology, School of Biotechnology, Division of Microbiology, SE-106 91, Stockholm, Sweden.
authors.forename: Karolin
authors.initials: K
authors.lastname: Axelsson
authors.affiliation: Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Ecological Chemistry Group, SE-100 44, Stockholm, Sweden.
authors.forename: Vera
authors.initials: V
authors.lastname: Konstanzer
authors.affiliation: Royal Institute of Technology, School of Biotechnology, Division of Microbiology, SE-106 91, Stockholm, Sweden.
authors.forename: Gunaratna Kuttuva
authors.initials: GK
authors.lastname: Rajarao
authors.affiliation: Swedish University of Agricultural Sciences, Department of Ecology, P.O. Box 7044, SE-750 07, Uppsala, Sweden.
authors.forename: Olle
authors.initials: O
authors.lastname: Terenius
authors.affiliation: Royal Institute of Technology, School of Biotechnology, Division of Microbiology, SE-106 91, Stockholm, Sweden.
authors.forename: Lisa
authors.initials: L
authors.lastname: Seriot
authors.affiliation: Swedish University of Agricultural Sciences, Department of Ecology, P.O. Box 7044, SE-750 07, Uppsala, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Nordenhem
authors.affiliation: Swedish University of Agricultural Sciences, Department of Ecology, P.O. Box 7044, SE-750 07, Uppsala, Sweden.
authors.forename: Göran
authors.initials: G
authors.lastname: Nordlander
authors.affiliation: Royal Institute of Technology, School of Chemical Science and Engineering, Department of Chemistry, Ecological Chemistry Group, SE-100 44, Stockholm, Sweden. akbk@kth.se.|Institute of Technology, Division of Organic Chemistry, Nooruse 1, Tartu University, Tartu, Estonia. akbk@kth.se.
authors.forename: Anna-Karin
authors.initials: AK
authors.lastname: Borg-Karlson
mesh_terms: D000818:Animals; D001419:Bacteria / Q000737:chemistry*; D005247:Feeding Behavior; D008401:Gas Chromatography-Mass Spectrometry; D060434:Herbivory; D028223:Pinus; D052617:Solid Phase Microextraction; D055549:Volatile Organic Compounds / Q000737:chemistry*; D048889:Weevils / Q000382:microbiology* / Q000502:physiology
chemical_list: D055549:Volatile Organic Compounds
keywords: 2-Methoxyphenol; 2-Phenylethanol; Antifeedant; Brevundimonas; Hylobius abietis; Rahnella