title: Isolation and characterisation of cytotoxic compounds from Euphorbia clementei Boiss.
abstract: A new phorbol-type diterpene ester, 4,20-dideoxy-4α-phorbol-12β-acetate-13α-isobutyrate, in addition to 11 known compounds were isolated from the latex and roots of Euphorbia clementei Boiss. Structure elucidation was performed by comprehensive 1D and 2D NMR analyses (1H and13C NMR, COSY, ROESY, HSQC and HMBC experiments), mass spectrometry (HR-ESI-MS) and by comparison with literature data. The inhibitory activity of all isolated compounds was evaluated against promyelocytic leukemia HL60 and human erythromyeloblastoid leukemia K562 cell lines and seven of these compounds exhibited a weak cytotoxicity with IC50values ranging from 40 to 97 μM.
authors.affiliation: a Laboratoire d'Obtention des Substances Thérapeutiques (LOST), Département de chimie , Université des frères Mentouri-Constantine , Constantine , Algeria.|b ICMR-UMR CNRS 7312 , Groupe Isolement et Structure , Reims , France.
authors.forename: Abbes
authors.initials: A
authors.lastname: Benmerache
authors.affiliation: b ICMR-UMR CNRS 7312 , Groupe Isolement et Structure , Reims , France.
authors.forename: Abdulmagid
authors.initials: A
authors.lastname: Alabdul Magid
authors.affiliation: a Laboratoire d'Obtention des Substances Thérapeutiques (LOST), Département de chimie , Université des frères Mentouri-Constantine , Constantine , Algeria.
authors.forename: Amira
authors.initials: A
authors.lastname: Labed
authors.affiliation: a Laboratoire d'Obtention des Substances Thérapeutiques (LOST), Département de chimie , Université des frères Mentouri-Constantine , Constantine , Algeria.
authors.forename: Ahmed
authors.initials: A
authors.lastname: Kabouche
authors.affiliation: b ICMR-UMR CNRS 7312 , Groupe Isolement et Structure , Reims , France.
authors.forename: Laurence
authors.initials: L
authors.lastname: Voutquenne-Nazabadioko
authors.affiliation: b ICMR-UMR CNRS 7312 , Groupe Isolement et Structure , Reims , France.
authors.forename: Jane
authors.initials: J
authors.lastname: Hubert
authors.affiliation: c Faculté de Pharmacie, MEDyC UMR CNRS 7369 , URCA , Reims , France.
authors.forename: Hamid
authors.initials: H
authors.lastname: Morjani
authors.affiliation: a Laboratoire d'Obtention des Substances Thérapeutiques (LOST), Département de chimie , Université des frères Mentouri-Constantine , Constantine , Algeria.
authors.forename: Zahia
authors.initials: Z
authors.lastname: Kabouche
mesh_terms: D000972:Antineoplastic Agents, Phytogenic / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D004224:Diterpenes / Q000737:chemistry / Q000302:isolation & purification; D004354:Drug Screening Assays, Antitumor; D028481:Euphorbia / Q000737:chemistry*; D018922:HL-60 Cells; D006801:Humans; D020128:Inhibitory Concentration 50; D020014:K562 Cells; D007840:Latex / Q000737:chemistry; D009682:Magnetic Resonance Spectroscopy; D015394:Molecular Structure; D010704:Phorbols / Q000737:chemistry; D018517:Plant Roots / Q000737:chemistry; D021241:Spectrometry, Mass, Electrospray Ionization
chemical_list: C000624335:4,20-dideoxy-4alpha-phorbol-12beta-acetate-13alpha-isobutyrate; D000972:Antineoplastic Agents, Phytogenic; D004224:Diterpenes; D007840:Latex; D010704:Phorbols; C033085:phorbol
keywords: Euphorbia clementei; Euphorbiaceae; cytotoxic activity; diterpenoids; triterpenoids