title: Structures of biomolecular complexes by combination of NMR and cryoEM methods.
abstract: CryoEM is presently providing structures of biocomplexes considered intractable to analysis by other structural techniques. NMR is playing an important role in delivering structural information on dynamics events and conformational heterogeneity. Impressive results were obtained by combining cryoEM and either liquid- or solid-state NMR, revealing the structures of cellular machines, filaments and amyloid fibrils. NMR solution structures of proteins and nucleic acids were fitted, together with crystallographic structures, into cryoEM maps of large complexes, to decipher their assembly mechanisms and describe their functional dynamics. Modelling based on solid-state NMR and cryoEM data provided 3D structure of filaments and fibrils. These NMR approaches validated, but also corrected, atomic models built de novo in cryoEM maps, and provided new structural data on flexible or structurally heterogeneous systems. Combination of cryoEM and NMR became an established hybrid approach in structural biology that significantly contributes to our understanding of functional mechanisms in supramolecular assemblies.
authors.affiliation: Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Univ. Paris Sud, Université Paris-Saclay, F-91198 Gif-sur-Yvette, France; Institut de Biologie et de Technologies de Saclay, CEA, F-91191 Gif-sur-Yvette, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Cuniasse
authors.affiliation: Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Univ. Paris Sud, Université Paris-Saclay, F-91198 Gif-sur-Yvette, France.
authors.forename: Paulo
authors.initials: P
authors.lastname: Tavares
authors.affiliation: Institute of Structural and Molecular Biology, Birkbeck College, London WC1E 7HX, United Kingdom.
authors.forename: Elena V
authors.initials: EV
authors.lastname: Orlova
authors.affiliation: Institut de Biologie Intégrative de la Cellule (I2BC), CEA, CNRS, Univ. Paris Sud, Université Paris-Saclay, F-91198 Gif-sur-Yvette, France; Institut de Biologie et de Technologies de Saclay, CEA, F-91191 Gif-sur-Yvette, France. Electronic address: sophie.zinn@cea.fr.
authors.forename: Sophie
authors.initials: S
authors.lastname: Zinn-Justin
mesh_terms: D000682:Amyloid / Q000737:chemistry; D020285:Cryoelectron Microscopy / Q000379:methods*; D006801:Humans; D046911:Macromolecular Substances / Q000737:chemistry*; D019906:Nuclear Magnetic Resonance, Biomolecular / Q000379:methods*; D014771:Virion / Q000737:chemistry
chemical_list: D000682:Amyloid; D046911:Macromolecular Substances