title: Association of HLA-DRB1 and HLA-DQB1 with red-blood-cell alloimmunization in the Czech population.
abstract: OBJECTIVE
  Alloimmune antibodies against red-blood-cell (RBC) antigens induced in susceptible individuals (responders) by transfusion, pregnancy or transplantation may have serious clinical consequences. The aim of this study was to investigate association of alloimmunization against selected RBC antigens with HLA-Class II.
  
  
  METHODS
  A total of 230 responders (106 monoresponders and 124 multiresponders) were enrolled into the study. HLA-DRB1 and HLA-DQB1 variants were determined by PCR-SSO and their frequencies compared between the patients (patient subgroups) and 375 ethnically and regionally matched controls.
  
  
  RESULTS
  Development of multiple RBC antibodies was associated with HLA-DRB1*15 and HLA-DQB1*06 allelic groups in the patients, with the relationship being particularly apparent in those with anti-C+D antibodies. Furthermore, DRB1*13 and DQB1*06 were more frequent in multiresponders with anti-E+c antibodies and DRB1*03 and DQB1*02 in those with anti-E+Cw.
  
  
  CONCLUSIONS
  For the first time, we confirmed the association of HLA-DRB1*15 with RBC antibody multiresponder status and found HLA-Class II associations for three frequent RBC antibody combinations. Our data support the concept that HLA restriction plays an important role in the response to RBC alloantigens.
authors.affiliation: Blood Centre, University Hospital, Ostrava, Czech Republic.
authors.forename: A
authors.initials: A
authors.lastname: Maluskova
authors.affiliation: Department of Immunology, Faculty of Medicine and Dentistry, Palacky University, Olomouc, Czech Republic.
authors.forename: F
authors.initials: F
authors.lastname: Mrazek
authors.affiliation: Blood Centre, University Hospital, Ostrava, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Pauliskova
authors.affiliation: Blood Centre, University Hospital, Ostrava, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Kovarova
authors.affiliation: Blood Centre, University Hospital, Ostrava, Czech Republic.
authors.forename: M
authors.initials: M
authors.lastname: Koristka
authors.affiliation: Department of Haematology and Oncology, University Hospital, Plzen, Czech Republic.
authors.forename: P
authors.initials: P
authors.lastname: Jindra
authors.affiliation: Blood Centre, University Hospital, Ostrava, Czech Republic.|Faculty of Medicine, Ostrava University, Ostrava, Czech Republic.
authors.forename: Z
authors.initials: Z
authors.lastname: Cermakova
mesh_terms: D000328:Adult; D000483:Alleles; D018153:Czech Republic; D004912:Erythrocytes / Q000276:immunology*; D005260:Female; D005787:Gene Frequency; D005838:Genotype; D059866:HLA-DQ beta-Chains / Q000235:genetics / Q000276:immunology*; D059811:HLA-DRB1 Chains / Q000235:genetics / Q000276:immunology*; D006239:Haplotypes; D006801:Humans; D007518:Isoantibodies / Q000097:blood; D008297:Male; D011247:Pregnancy
chemical_list: D059866:HLA-DQ beta-Chains; C069051:HLA-DQB1 antigen; D059811:HLA-DRB1 Chains; D007518:Isoantibodies
keywords: HLA; red-blood-cell antigens and antibodies; transfusion medicine (in general)