title: Utility of11C-methionine and11C-donepezil for imaging ofStaphylococcus aureusinduced osteomyelitis in a juvenile porcine model: comparison to autologous111In-labelled leukocytes,99mTc-DPD, and18F-FDG.
abstract: The aim of this study was to compare11C-methionine and11C-donepezil positron emission tomography (PET) with111In-labeled leukocyte and99mTc-DPD (Tc-99m 3,3-diphosphono-1,2-propanedicarboxylic acid) single-photon emission computed tomography (SPECT), and18F-fluorodeoxyglucose (18F-FDG) PET to improve detection of osteomyelitis. The tracers' diagnostic utility where tested in a juvenile porcine hematogenously induced osteomyelitis model comparable to osteomyelitis in children. Five 8-9 weeks old female domestic pigs were scanned seven days after intra-arterial inoculation in the right femoral artery with a porcine strain ofStaphylococcus aureus. The sequential scan protocol included Computed Tomography,11C-methionine and11C-donepezil PET,99mTc-DPD and111In-labelled leukocytes scintigraphy, and18F-FDG PET. This was followed by necropsy of the pigs and gross pathology, histopathology, and microbial examination. The pigs developed a total of 24 osteomyelitic lesions, 4 lesions characterized as contiguous abscesses and pulmonary abscesses (in two pigs). By comparing the 24 osteomyelitic lesions,18F-FDG accumulated in 100%,111In-leukocytes in 79%,11C-methionine in 79%,11C-donepezil in 58%, and99mTc-DPD in none. Overall,18F-FDG PET was superior to111In-leukocyte SPECT and11C-methionine in marking infectious lesions.
authors.affiliation: Department of Diagnostic Imaging, North Zealand Hospital, Hillerød, University Hospital of Copenhagen Copenhagen, Denmark.
authors.forename: Pia
authors.initials: P
authors.lastname: Afzelius
authors.affiliation: Department of Nuclear Medicine and PET Centre, Aarhus University Hospital Aarhus, Denmark.
authors.forename: Aage Ko
authors.initials: AK
authors.lastname: Alstrup
authors.affiliation: Department of Clinical Microbiology, Aalborg University HospitalAalborg, Denmark; Department of Clinical Medicine, Aalborg UniversityAalborg, Denmark.
authors.forename: Henrik C
authors.initials: HC
authors.lastname: Schønheyder
authors.affiliation: Department of Nuclear Medicine and PET Centre, Aarhus University Hospital Aarhus, Denmark.
authors.forename: Per
authors.initials: P
authors.lastname: Borghammer
authors.affiliation: Department of Nuclear Medicine, Aalborg University HospitalAalborg, Denmark; Department of Chemistry and Biochemistry, Aalborg UniversityAalborg, Denmark.
authors.forename: Svend B
authors.initials: SB
authors.lastname: Jensen
authors.affiliation: Department of Nuclear Medicine and PET Centre, Aarhus University Hospital Aarhus, Denmark.
authors.forename: Dirk
authors.initials: D
authors.lastname: Bender
authors.affiliation: Department of Veterinary Disease Biology, University of Copenhagen Copenhagen, Denmark.
authors.forename: Ole L
authors.initials: OL
authors.lastname: Nielsen
keywords: 111In-labelled leukocytes; 11C-donepezil; 11C-methionine; 18F-FDG; 99mTc-DPD; CT; PET/CT; SPECT/CT; Staphylococcus aureus; osteomyelitis; pig; porcine; scintigraphy; swine