title: Leptin, Leptin Soluble Receptor, and the Free Leptin Index following a Diet and Physical Activity Lifestyle Intervention in Obese Males and Females.
abstract: Leptin (LEP) is associated with appetite regulation and metabolism. Concentration is linear with adiposity, suggesting LEP resistance. LEP circulates freely and bound with its soluble receptor (sOB-r); the ratio is the free leptin index (FLI), an index of leptin resistance; lower FLI suggests reduced biological action.Purpose. The aim was to determine the effect of changes in adipose tissue distribution on LEP, sOB-r, and FLI following 6 months (6 M) of a diet/exercise weight loss program (WLP). In addition, we aim to identify predictors of the FLI.Methods. 6 M WLP consisted of diet/lifestyle interventions following ADA guidelines. Body composition was assessed by DXA. LEP and sOB-r analysis were done via ELISA.Results. 10 adults completed the WLP. Significant reductions were seen in total fat percentage (% fat), nontrunk fat, (NTF), and trunk fat (TF) from base to 3 m and 6 M (p≤ 0.05). The FLI were reduced at 3 M and 6 M for males and 6 M for females. Total body fat and body weight predicted the FLI in both sexes.Conclusions. LEP and FLI reductions following 6 M of WLP were achieved independent of sOB-r changes. We also demonstrate that the FLI can be predicted noninvasively through total fat mass and body weight in kilograms.
authors.affiliation: Department of Rehabilitation Science, George Mason University, 4400 University Drive, MS 2G7, Fairfax, VA 22030, USA.
authors.forename: Jeffrey E
authors.identifier: 0000-0002-6466-0679
authors.initials: JE
authors.lastname: Herrick
authors.affiliation: Department of Rehabilitation Science, George Mason University, 4400 University Drive, MS 2G7, Fairfax, VA 22030, USA.
authors.forename: Gino S
authors.initials: GS
authors.lastname: Panza
authors.affiliation: Department of Rehabilitation Science, George Mason University, 4400 University Drive, MS 2G7, Fairfax, VA 22030, USA.
authors.forename: Jared M
authors.initials: JM
authors.lastname: Gollie
mesh_terms: D015992:Body Mass Index; D004038:Diet, Reducing; D015444:Exercise; D005260:Female; D006801:Humans; D020738:Leptin / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D009765:Obesity / Q000097:blood / Q000178:diet therapy / Q000628:therapy*; D054411:Receptors, Leptin / Q000378:metabolism*; D016896:Treatment Outcome
chemical_list: C515448:LEPR protein, human; D020738:Leptin; D054411:Receptors, Leptin