title: Superovulatory responses in cynomolgus monkeys (Macaca fascicularis) depend on the interaction between donor status and superovulation method used.
abstract: The current study was performed to investigate the effect of oocyte donor status, including age and body weight, on metaphase II (MII) oocyte recovery using two superovulation methods in cynomolgus monkeys. The use of Method A [recombinant gonadotrophin (75 IU/kg, 3 ×, 3-day intervals) and human chorionic gonadotropin (hCG)] led to great increases in ovary size and the mean number of MII oocytes retrieved in age- and body-weight-dependent manner; in contrast, both the parameters were similar in Method B [recombinant gonadotrophin (60 IU, twice daily, 6 days), recombinant gonadotropin and recombinant human luteinizing hormone (rhLH) (60 IU, twice daily, 3 days), and hCG]. Importantly, Method A showed maximal MII oocyte recovery rate in > 60-month-old or 4.5-5.0-kg female monkeys, whereas Method B was equally effective regardless of the donor age and body weight. These results indicate that superovulatory responses depend on the interaction between oocyte donor status and the superovulation method used in cynomolgus monkeys.
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Department of Functional Genomics, University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Ji-Su
authors.initials: JS
authors.lastname: Kim
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Department of Functional Genomics, University of Science and Technology, Daejeon 34113, Republic of Korea.|Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Seung-Bin
authors.initials: SB
authors.lastname: Yoon
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Kang-Jin
authors.initials: KJ
authors.lastname: Jeong
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Bo-Woong
authors.initials: BW
authors.lastname: Sim
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Seon-A
authors.initials: SA
authors.lastname: Choi
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Sang-Il
authors.initials: SI
authors.lastname: Lee
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Yeung Bae
authors.initials: YB
authors.lastname: Jin
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Bong-Seok
authors.initials: BS
authors.lastname: Song
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Department of Functional Genomics, University of Science and Technology, Daejeon 34113, Republic of Korea.
authors.forename: Sang-Rae
authors.initials: SR
authors.lastname: Lee
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Department of Functional Genomics, University of Science and Technology, Daejeon 34113, Republic of Korea.|Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Sun-Uk
authors.initials: SU
authors.lastname: Kim
authors.affiliation: National Primate Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.|Department of Functional Genomics, University of Science and Technology, Daejeon 34113, Republic of Korea.|Futuristic Animal Resource & Research Center, Korea Research Institute of Bioscience and Biotechnology, Cheongju 28116, Republic of Korea.
authors.forename: Kyu-Tae
authors.initials: KT
authors.lastname: Chang
mesh_terms: D000818:Animals; D006063:Chorionic Gonadotropin / Q000008:administration & dosage; D054885:Cumulus Cells / Q000187:drug effects / Q000502:physiology; D005260:Female; D005640:Follicle Stimulating Hormone / Q000008:administration & dosage; D007986:Luteinizing Hormone / Q000008:administration & dosage; D008252:Macaca fascicularis; D054315:Oocyte Retrieval / Q000379:methods / Q000662:veterinary*; D009865:Oocytes / Q000187:drug effects / Q000502:physiology*; D010062:Ovulation Induction / Q000379:methods / Q000662:veterinary*; D013480:Superovulation / Q000187:drug effects / Q000502:physiology*
chemical_list: D006063:Chorionic Gonadotropin; D007986:Luteinizing Hormone; D005640:Follicle Stimulating Hormone
keywords: Age; Body weight; Cynomolgus monkey; MII oocyte; Superovulation