title: Extracellular vesicle mediated intercellular communication at the porcine maternal-fetal interface: A new paradigm for conceptus-endometrial cross-talk.
abstract: Exosomes and microvesicles are extracellular vesicles released from cells and can contain lipids, miRNAs and proteins that affect cells at distant sites. Recently, microvesicles containing miRNA have been implicated in uterine microenvironment of pigs, a species with unique epitheliochorial (non-invasive) placentation. Here we report a novel role of conceptus-derived exosomes/microvesicles (hereafter referred to as extracellular vesicles; EVs) in embryo-endometrial cross-talk. We also demonstrate the stimulatory effects of EVs (PTr2-Exo) derived from porcine trophectoderm-cells on various biological processes including the proliferation of maternal endothelial cells (PAOEC), potentially promoting angiogenesis. Transmission immuno-electron microscopy confirmed the presence of EVs in tissue biopsies, PTr2-Exo and PAOEC-derived EVs (PAOEC-Exo). RT-PCR detected 14 select miRNAs in CD63 positive EVs in which miR-126-5P, miR-296-5P, miR-16, and miR-17-5P were the most abundant angiogenic miRNAs. Proteomic analysis revealed EV proteins that play a role in angiogenesis. In-vitro experiments, using two representative cell lines of maternal-fetal interface, demonstrated bidirectional EVs shuttling between PTr2 and PAOEC cells. Importantly, these studies support the idea that PTr2-Exo and PAOEC-Exo containing select miRNAs and proteins can be successfully delivered to recipient cells and that they may have a biological role in conceptus-endometrial cross-talk crucial for the pregnancy success.
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
authors.forename: Mallikarjun
authors.initials: M
authors.lastname: Bidarimath
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.|Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Kasra
authors.initials: K
authors.lastname: Khalaj
authors.affiliation: Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.|Department of Animal Production, Faculty of Agriculture, Jordan University of Science and Technology, Irbid, 22110, Jordan.
authors.forename: Rami T
authors.initials: RT
authors.lastname: Kridli
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
authors.forename: Frederick W K
authors.initials: FW
authors.lastname: Kan
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.
authors.forename: Madhuri
authors.initials: M
authors.lastname: Koti
authors.affiliation: Department of Biomedical and Molecular Sciences, Queen's University, Kingston, Ontario, K7L 3N6, Canada.|Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario, N1G 2W1, Canada.
authors.forename: Chandrakant
authors.initials: C
authors.lastname: Tayade
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D002450:Cell Communication; D049109:Cell Proliferation; D049033:Chorioallantoic Membrane / Q000378:metabolism / Q000648:ultrastructure; D004717:Endometrium / Q000166:cytology* / Q000378:metabolism; D042783:Endothelial Cells / Q000166:cytology; D055354:Exosomes / Q000378:metabolism; D000067128:Extracellular Vesicles / Q000378:metabolism* / Q000648:ultrastructure; D005260:Female; D005333:Fetus / Q000166:cytology*; D008431:Maternal-Fetal Exchange; D035683:MicroRNAs / Q000378:metabolism; D008954:Models, Biological; D011247:Pregnancy; D040901:Proteomics; D034421:Sus scrofa; D060149:Tetraspanin 30 / Q000378:metabolism; D014327:Trophoblasts / Q000166:cytology
chemical_list: D015415:Biomarkers; D035683:MicroRNAs; D060149:Tetraspanin 30