title: Chamomile (Matricaria recutita L.) decoction extract inhibits in vitro intestinal glucose absorption and attenuates high fat diet-induced lipotoxicity and oxidative stress.
abstract: The present study aimed to investigate the inhibitory effect of chamomile decoction extract (CDE) on intestinal glucose absorption as well as its protective role against high fat diet (HFD)-induced obesity and lipotoxicity in rats. We used the Ussing chamber system to investigate the effect of CDE on intestinal transport of glucose. Male Wistar rats were fed HFD for six weeks to provoke obesity. CDE (100mg/kg, b.w. p.o.) has been per orally administered to HFD fed rats. Ex vivo, we found that CDE significantly and dose-dependently increased intestinal absorption of glucose. In vivo, HFD increased the body, liver and kidney weights, while CDE treatment showed a significant protective effects. High fat diet induced also a lipid profiles disorder and a disturbances in kidney and liver function parameters. Moreover liver and kidney lipotoxicity is accompanied by an oxidative stress status characterized by increased lipoperoxidation, depletion of antioxidant enzymes activity and non-enzymatic antioxidant (-SH groups and GSH) levels as well as increased levels of free iron, hydrogen peroxide (H2O2) and calcium. However, treatment with CDE alleviated all the deleterious effects of HFD feed. These findings suggest that chamomile decoction extract can be used as functional beverage against obesity, hyperglycemia and hyperlipidemia.
authors.affiliation: Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisie; Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources, Institut Supérieur de Biotechnologie de Béja, Avenue Habib Bourguiba, B.P. 382, 9000 Béja, Université de Jendouba, Tunisie. Electronic address: jabri.amino@gmail.com.
authors.forename: Mohamed-Amine
authors.initials: MA
authors.lastname: Jabri
authors.affiliation: Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisie.
authors.forename: Mohsen
authors.initials: M
authors.lastname: Sakly
authors.affiliation: Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources, Institut Supérieur de Biotechnologie de Béja, Avenue Habib Bourguiba, B.P. 382, 9000 Béja, Université de Jendouba, Tunisie.
authors.forename: Lamjed
authors.initials: L
authors.lastname: Marzouki
authors.affiliation: Laboratoire de Physiologie Intégrée, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisie; Laboratoire de Physiologie Fonctionnelle et Valorisation des Bio-Ressources, Institut Supérieur de Biotechnologie de Béja, Avenue Habib Bourguiba, B.P. 382, 9000 Béja, Université de Jendouba, Tunisie.
authors.forename: Hichem
authors.initials: H
authors.lastname: Sebai
mesh_terms: D000818:Animals; D020843:Chamomile; D059305:Diet, High-Fat / Q000009:adverse effects*; D005947:Glucose / Q000037:antagonists & inhibitors / Q000378:metabolism*; D007408:Intestinal Absorption / Q000187:drug effects / Q000502:physiology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology*; D008297:Male; D031168:Matricaria; D051379:Mice; D009924:Organ Culture Techniques; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D010936:Plant Extracts; D005947:Glucose
keywords: Free iron; Intestinal absorption; Lipotoxicity oxidative stress; Ussing chamber