title: Characterization of haloalkalophilic organic solvent tolerant protease for chitin extraction from shrimp shell waste.
abstract: Halophilic organic solvent tolerant protease (HOSP) producing Paracoccus saliphilus APCMST-CS5 was isolated from the marine sediment samples and identified through 16S rRNA sequence analysis. P. saliphilus APCMST-CS5 registered maximum HOSP production of 1,321.70U/ml in the medium contained the most significant parameters such as shrimp shell powder (SSP), CaCl2, NaCl, and sardinella powder (SP), obtained through Placket-Burman and Response Surface Methods. HOSP was further purified to 22.68 fold purity with 29.71 U/mg specific activity and its molecular weight was 39kDa. The HOSP was stable at 60°C, 9.0 pH, 3.0M NaCl concentration and it also showed maximum activity at other tested parameters. Interestingly the purified HOSP showed better antibiofilm ability against tested pathogens. Also, the HOSP effectively deproteinized (85.64%) shrimp shell chitin which in turn maximum and exhibited higher antioxidant activity. The commercial and experimental shrimp shell chitin showed similar peak pattern in FTIR and13C CP/MAS NMR spectral analysis.
authors.affiliation: Centre for Marine Science and Technology, Manonmaniam Sundaranar University, Rajakkamangalam-629 502, Kanyakumari District, Tamilnadu, India.
authors.forename: Thirumalai
authors.initials: T
authors.lastname: Maruthiah
authors.affiliation: Department of Animal Science, Manonmaniam Sundaranar University, Tirunelveli 627 012, Tamilnadu, India. Electronic address: plavesh06@gmail.com.
authors.forename: Arunachalam
authors.initials: A
authors.lastname: Palavesam
mesh_terms: D060105:Animal Shells / Q000737:chemistry*; D000818:Animals; D000975:Antioxidants / Q000737:chemistry / Q000302:isolation & purification; D018441:Biofilms / Q000187:drug effects; D001713:Biphenyl Compounds / Q000737:chemistry; D002686:Chitin / Q000737:chemistry / Q000302:isolation & purification*; D033364:Decapoda / Q000737:chemistry*; D004795:Enzyme Stability / Q000187:drug effects; D019015:Geologic Sediments / Q000382:microbiology; D008970:Molecular Weight; D009930:Organic Chemicals / Q000494:pharmacology*; D010230:Paracoccus / Q000201:enzymology / Q000235:genetics / Q000302:isolation & purification; D010447:Peptide Hydrolases / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology; D010851:Picrates / Q000737:chemistry; D059748:Proteolysis / Q000187:drug effects; D012336:RNA, Ribosomal, 16S / Q000235:genetics; D012492:Salts / Q000494:pharmacology; D012997:Solvents / Q000494:pharmacology*; D013223:Statistics as Topic; D014866:Waste Products
chemical_list: D000975:Antioxidants; D001713:Biphenyl Compounds; D009930:Organic Chemicals; D010851:Picrates; D012336:RNA, Ribosomal, 16S; D012492:Salts; D012997:Solvents; D014866:Waste Products; D002686:Chitin; C004931:1,1-diphenyl-2-picrylhydrazyl; D010447:Peptide Hydrolases
keywords: (13)C CP/MAS NMR; FTIR; HOSP; Marine fish wastes; Shrimp shell chitin