title: Validation data supporting the characterization of novel copper complexes as anticancer agents.
abstract: Three copper(II) complexes, Cu(Sal-Gly)(phen), Cu(Sal-Gly)pheamine, Cu(Sal-Gly)phepoxy were synthesized and characterized for their anticancer properties and mechanism of action (Acilan et al., in press) [1]. Here, we provide supporting data on colon cancer cell lines complementing our previous findings in cervix cells. This paper also contains a data table for the fold changes andp-values of all genes analyzed in this study via a custom RT-qPCR array. All compounds induced DNA damage (based on 8-oxo-guanidine, ɣH2AX staining in cells) and apoptosis (based on elevated DNA condensation/fragmentation, Annexin V staining, caspase 3/7 activity and mitochondrial membrane depolarization) in HCT-116 colon cancer cells. The increase in oxidative stress was also further confirmed in these cells. Further interpretation of the data presented here can be found in the article entitled "Synthesis, biological characterization and evaluation of molecular mechanisms of novel copper complexes as anticancer agents" (Acilan et al., in press) [1].
authors.affiliation: TUBITAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey.
authors.forename: Ceyda
authors.initials: C
authors.lastname: Acilan
authors.affiliation: Uludag University, Faculty of Arts and Sciences, Department of Biology, Bursa, Turkey.
authors.forename: Buse
authors.initials: B
authors.lastname: Cevatemre
authors.affiliation: TUBITAK, Marmara Research Center, Genetic Engineering and Biotechnology Institute, Gebze, Kocaeli, Turkey.
authors.forename: Zelal
authors.initials: Z
authors.lastname: Adiguzel
authors.affiliation: Uludag University, Faculty of Arts and Sciences, Department of Biology, Bursa, Turkey.
authors.forename: Didem
authors.initials: D
authors.lastname: Karakas
authors.affiliation: Uludag University, Medical School, Department of Medical Biochemistry, Bursa, Turkey.
authors.forename: Engin
authors.initials: E
authors.lastname: Ulukaya
authors.affiliation: Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisbon, Portugal.
authors.forename: Nádia
authors.initials: N
authors.lastname: Ribeiro
authors.affiliation: Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisbon, Portugal.
authors.forename: Isabel
authors.initials: I
authors.lastname: Correia
authors.affiliation: Centro de Química Estrutural, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais 1, 1049-001 Lisbon, Portugal.
authors.forename: João Costa
authors.initials: JC
authors.lastname: Pessoa