title: Extracellular matrix metalloproteinase inducer enhances host resistance against pseudomonas aeruginosa infection through MAPK signaling pathway.
abstract: This study aims to explore the role of extra-cellular matrix metalloproteinase inducer (EMMPRIN) in the drug resistance of the pseudomonas aeruginosa (PA). The BALB/c mice were transfected with PA, then the mice were infected with the siRNA of EMMPRIN to silence the EMMPRIN gene. The EMMPRIN mRNA and protein were detected by using RT-PCR and western blot, respectively. In order to examine the function of EMMPRIN in drug resistance of PA, the BALB/c and C57BL/6 mice were treated with EMMPRIN siRNA. The cytokines, EMMPRIN and MMP9 were examined by the RP-PCR and ELISA, respectively, undergoing the silence of EMMPRIN siRNA. Moreover, the western blot assay was also used to test the phosphorylated MAPK in the murine macrophages after silenced by the EMMPRIN siRNA. The EMMPRIN was activated, with lipopolysaccharide stimulation and treated with the MAPK inhibitor, to evaluate whether the MAPK participates in the EMMPRIN-triggered drug resistance. The results indicated that the EMMPRIN expression was elevated in the infected BALB/c at 3 or 5 days post-infection. Silence of EMMPRIN Enhanced the Production of pro-inflammatory cytokines in PA keratitis. Silence of EMMPRIN significantly up-regulated Th1-type cytokines IFN-γ, IL-12, and IL-18, but down-regulated Th2-type cytokines IL-4, IL-5, and IL-10. MMP9 was increased in the cells with rEMMPRIN treatment. EMMPRIN inhibits pro-inflammatory cytokine production via a MAPK signaling pathway. In conclusion, EMMPRIN promotes host resistance against pseudomonas aeruginosa infection via MAPK signaling pathway.
authors.affiliation: Wuhan University School of Public HealthWuhan 430071, China; Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese MedicineZhengzhou 450002, China.
authors.forename: Yongwei
authors.initials: Y
authors.lastname: Li
authors.affiliation: Beijing University of Chinese Medicine Beijing 100029, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
authors.forename: Chunxia
authors.initials: C
authors.lastname: Wang
authors.affiliation: Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
authors.forename: Jianshe
authors.initials: J
authors.lastname: Chen
authors.affiliation: Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
authors.forename: Xiaoqian
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
authors.forename: Xiaobin
authors.initials: X
authors.lastname: Niu
authors.affiliation: Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
authors.forename: Dongxu
authors.initials: D
authors.lastname: Pei
authors.affiliation: Department of Clinical Laboratory, Henan Province Hospital of Traditional Chinese Medicine Zhengzhou 450002, China.
authors.forename: Zhiqiang
authors.initials: Z
authors.lastname: He
authors.affiliation: Wuhan University School of Public Health Wuhan 430071, China.
authors.forename: Yongyi
authors.initials: Y
authors.lastname: Bi
keywords: EMMPRIN; MAPK; durg resistance; pseudomonas aeruginosa