title: A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay.
abstract: Actinobacillus pleuropneumoniaecauses pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates ofA. pleuropneumoniaethat were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85/14 combined with the previous serological data show the existence of a sixteenth serovar-designated serovar 16-ofA. pleuropneumoniae.
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom j.bosse@imperial.ac.uk p.langford@imperial.ac.uk.
authors.forename: Janine T
authors.identifier: 0000-0002-8491-4779
authors.initials: JT
authors.lastname: Bossé
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom.
authors.forename: Yanwen
authors.initials: Y
authors.lastname: Li
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: Rita
authors.initials: R
authors.lastname: Sárközi
authors.affiliation: Groupe de Recherche sur les Maladies Infectieuses du Porc, Faculté de Médecine Vétérinaire, Université de Montréal, Montréal, Québec, Canada.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.affiliation: Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark.
authors.forename: Øystein
authors.initials: Ø
authors.lastname: Angen
authors.affiliation: Veterinary Research Institute, Brno, Czech Republic.
authors.forename: Katerina
authors.initials: K
authors.lastname: Nedbalcova
authors.affiliation: Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, Hatfield, United Kingdom.
authors.forename: Andrew N
authors.initials: AN
authors.lastname: Rycroft
authors.affiliation: Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary.
authors.forename: László
authors.initials: L
authors.lastname: Fodor
authors.affiliation: Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom j.bosse@imperial.ac.uk p.langford@imperial.ac.uk.
authors.forename: Paul R
authors.identifier: 0000-0002-6368-4724
authors.initials: PR
authors.lastname: Langford
mesh_terms: D000189:Actinobacillus Infections / Q000175:diagnosis / Q000662:veterinary*; D016977:Actinobacillus pleuropneumoniae / Q000145:classification* / Q000235:genetics; D000818:Animals; D016667:Bacterial Capsules / Q000235:genetics*; D017931:DNA Primers / Q000235:genetics; D004269:DNA, Bacterial / Q000737:chemistry / Q000235:genetics; D056426:Genetic Loci; D016680:Genome, Bacterial; D025202:Molecular Diagnostic Techniques / Q000379:methods; D011001:Pleuropneumonia / Q000382:microbiology / Q000662:veterinary; D016133:Polymerase Chain Reaction / Q000379:methods*; D017422:Sequence Analysis, DNA; D065288:Serogroup; D013552:Swine; D013553:Swine Diseases / Q000175:diagnosis*
chemical_list: D017931:DNA Primers; D004269:DNA, Bacterial
keywords: Actinobacillus pleuropneumoniae; PCR; diagnostics; serovar 16