title: Cytotoxic, Antiproliferative and Apoptotic Effects of Perillyl Alcohol and Its Biotransformation Metabolite on A549 and HepG2 Cancer Cell Lines.
abstract: BACKGROUND
  A monoterpene, perillyl alcohol, has attracted attention in medicinal chemistry since it exhibited chemo-preventive and therapeutic properties against a variety of cancers.
  
  
  OBJECTIVE
  In the present work, it was aimed to obtain derivatives of perillyl alcohol through microbial biotransformation and investigate their anticancer activities against A549 and HepG2 cancer cell lines.
  
  
  METHODS
  Biotransformation studies were carried out in a α-medium for 7 days at 25oC. XTT assay was performed to investigate the anticancer activities of perillyl alcohol and its biotransformation metabolite, dehydroperillic acid, against A549 and HepG2 cell lines and their selectivity using healthy cell line, NIH/3T3. Cell proliferation ELISA, BRDU (colorimetric) assay was used for measurement of proliferation in replicative cells in which DNA synthesis occurs. Flow cytometric analyses were also carried out for measuring apoptotic cell percentages, caspase 3 activation and mitochondrial membrane potential.
  
  
  RESULTS
  Biotransformation of perillyl alcohol with Fusarium culmorum yielded dehydroperillic acid in a yield of 20.4 %. In in vitro anticancer studies, perillyl alcohol was found to exert cytotoxicity against HepG2 cell line with an IC50 value of 409.2 μg/mL. However, this effect was not found to be selective because of its higher IC50 (250 μg/mL) value against NIH/3T3 cell line. On the other hand, dehydroperillic acid was found to be effective and also selective against A549 cell line with an IC50 value of 125 μg/mL and a selectivity index (SI) value of 400. Apoptosis inducing effects of dehydroperillic acid was better in A549 cell line.
  
  
  CONCLUSIONS
  Dehydroperillic acid may be a good candidate for therapy of lung adenocarcinoma and may show this anticancer activity by inducing apoptosis.
authors.affiliation: Department of Chemistry, Faculty of Science and Letters, Eskisehir Osmangazi University, 26480 Eskisehir. Turkey.
authors.forename: Ceren E
authors.initials: CE
authors.lastname: Oturanel
authors.affiliation: Department of Chemistry, Faculty of Science and Letters, Eskisehir Osmangazi University, 26480 Eskisehir. Turkey.
authors.forename: İsmail
authors.initials: İ
authors.lastname: Kıran
authors.affiliation: Department of Chemistry, Faculty of Science and Letters, Eskisehir Osmangazi University, 26480 Eskisehir. Turkey.
authors.forename: Özge
authors.initials: Ö
authors.lastname: Özşen
authors.affiliation: Department of Biochemistry, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir. Turkey.
authors.forename: Gülşen A
authors.initials: GA
authors.lastname: Çiftçi
authors.affiliation: Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Anadolu University, 26470 Eskisehir. Turkey.
authors.forename: Özlem
authors.initials: Ö
authors.lastname: Atlı
mesh_terms: D000818:Animals; D000970:Antineoplastic Agents / Q000138:chemical synthesis / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D001711:Biotransformation; D045744:Cell Line, Tumor; D049109:Cell Proliferation / Q000187:drug effects; D004305:Dose-Response Relationship, Drug; D004354:Drug Screening Assays, Antitumor; D005434:Flow Cytometry; D056945:Hep G2 Cells; D006801:Humans; D051379:Mice; D008968:Molecular Conformation; D039821:Monoterpenes / Q000737:chemistry / Q000378:metabolism* / Q000494:pharmacology*; D041681:NIH 3T3 Cells; D013329:Structure-Activity Relationship
chemical_list: D000970:Antineoplastic Agents; D039821:Monoterpenes; C032208:perillyl alcohol
keywords: Anticancer; antiproliferative; apoptosis; biotransformation; cytotoxicity; perillyl alcohol.