title: A simple and selective UHPLC-MS/MS method for quantification of plantagoguanidinic acid in rat plasma and its application to a pharmacokinetic study.
abstract: A simple, sensitive and specific UHPLC-MS/MS method for quantification of plantagoguanidinic acid (PGA) in rat plasma was applied to investigate the pharmacokinetic behavior in vivo, using protopine as internal standard. The chromatography was separated on a Phenomenex® Luna-C18column (2.1 × 150 mm, 3.0 μm) within 7.0 min using a mobile phase consisting of acetonitrile-0.1% formic acid solution under gradient elution at a flow rate of 0.4 mL/min. Prepared samples were monitored by multiple reaction monitoring mode, with the target fragmentions m/z 226.2 → 84.2 for PGA and m/z 354.2 → 188.9 for IS in positive electrospray ionization. The calibration curve of PGA was linear throughout the range 1-1000 ng/mL (r = 0.9962). The lower limit of quantitation in plasma for PGA was 0.1 ng/mL, and the recovery was >88.6%. Intra- and interday accuracy ranged from -8.6 to 4.9%. Furthermore, this validated method was successfully used for a pre-clinical pharmacokinetic study of PGA at a single dose of 20 and 5 mg/kg in rats via oral and intravenous administration. The study showed that PGA was absorpted rapidly and eliminated gradually with a greater absolute oral bioavailability of 70.1% in rats.
authors.affiliation: Jiangxi Institute for Drug Control, Nanchang, People's Republic of China.|School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Ruijian
authors.initials: R
authors.lastname: Zhong
authors.affiliation: Jiangxi Institute for Drug Control, Nanchang, People's Republic of China.|School of Pharmacy, Nanchang University, Nanchang, People's Republic of China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Yu
authors.affiliation: Jiangxi Institute for Drug Control, Nanchang, People's Republic of China.
authors.forename: Yangbing
authors.initials: Y
authors.lastname: Zheng
authors.affiliation: Jiangxi Institute for Drug Control, Nanchang, People's Republic of China.
authors.forename: Weikang
authors.identifier: http://orcid.org/0000-0001-8639-9910
authors.initials: W
authors.lastname: Chen
authors.affiliation: Jiangxi Institute for Drug Control, Nanchang, People's Republic of China.|School of Pharmacy, Nanchang University, Nanchang, People's Republic of China.|School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Guoping
authors.initials: G
authors.lastname: Zhou
authors.affiliation: Jiangxi Institute for Drug Control, Nanchang, People's Republic of China.|School of Pharmacy, Jiangxi University of Traditional Chinese Medicine, Nanchang, People's Republic of China.
authors.forename: Jianhong
authors.initials: J
authors.lastname: Ding
authors.affiliation: Jiangxi Institute for Drug Control, Nanchang, People's Republic of China.
authors.forename: Mingming
authors.initials: M
authors.lastname: Yuan
mesh_terms: D061605:Administration, Intravenous; D000284:Administration, Oral; D000470:Alkaloids / Q000097:blood*; D000818:Animals; D000893:Anti-Inflammatory Agents / Q000097:blood*; D001682:Biological Availability; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D004365:Drugs, Chinese Herbal / Q000493:pharmacokinetics; D006146:Guanidines / Q000097:blood*; D007004:Hypoglycemic Agents / Q000097:blood*; D057230:Limit of Detection; D008297:Male; D010943:Plantago / Q000737:chemistry*; D011620:Psyllium / Q000737:chemistry; D017207:Rats, Sprague-Dawley; D053719:Tandem Mass Spectrometry / Q000379:methods*
chemical_list: D000470:Alkaloids; D000893:Anti-Inflammatory Agents; D004365:Drugs, Chinese Herbal; D006146:Guanidines; D007004:Hypoglycemic Agents; C000621445:plantagoguanidinic acid; D011620:Psyllium
keywords: UHPLC-MS/MS; bioavailability; pharmacokinetics; plantagoguanidinic acid; rat plasma