title: Spatial mapping of metals in tissue-sections using combination of mass-spectrometry and histology through image registration.
abstract: We describe a new procedure for the parallel mapping of selected metals in histologically characterized tissue samples. Mapping is achieved via image registration of digital data obtained from two neighbouring cryosections by scanning the first as a histological sample and subjecting the second to laser ablation inductively coupled plasma mass spectrometry. This computer supported procedure enables determination of the distribution and content of metals of interest directly in the chosen histological zones and represents a substantial improvement over the standard approach, which determines these values in tissue homogenates or whole tissue sections. The potential of the described procedure was demonstrated in a pilot study that analysed Zn and Cu levels in successive development stages of pig melanoma tissue using MeLiM (Melanoma-bearing-Libechov-Minipig) model. We anticipate that the procedure could be useful for a complex understanding of the role that the spatial distribution of metals plays within tissues affected by pathological states including cancer.
authors.affiliation: Department of Cybernetics, Faculty of Electrical Engineering, Czech Technical University, Technicka 2, CZ-166 27 Prague 6, Czech Republic.
authors.forename: Jiri
authors.initials: J
authors.lastname: Anyz
authors.affiliation: Czech Institue of Informatics, Robotics, and Cybernetics, CTU in Prague, Jugoslávských partyzánů 1580/3, CZ-160 00 Prague 6, Czech Republic.
authors.forename: Lenka
authors.initials: L
authors.lastname: Vyslouzilova
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic.|Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic.
authors.forename: Tomas
authors.initials: T
authors.lastname: Vaculovic
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic.|Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic.
authors.forename: Michaela
authors.initials: M
authors.lastname: Tvrdonova
authors.affiliation: Department of Chemistry, Faculty of Science, Masaryk University, Kotlarska 2, CZ-611 37 Brno, Czech Republic.|Central European Institute of Technology, Masaryk University, Kamenice 5, CZ-625 00 Brno, Czech Republic.
authors.forename: Viktor
authors.initials: V
authors.lastname: Kanicky
authors.affiliation: Department of Food Chemistry and Toxicology, Berlin Institute of Technology, Gustav-Meyer-Allee 25, D-133 55 Berlin, Germany.
authors.forename: Hajo
authors.initials: H
authors.lastname: Haase
authors.affiliation: Laboratory of Tumour Biology, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, v.v.i., Rumburska 89, CZ-277 21 Libechov, Czech Republic.
authors.forename: Vratislav
authors.initials: V
authors.lastname: Horak
authors.affiliation: Department of Cybernetics, Faculty of Electrical Engineering, Czech Technical University, Technicka 2, CZ-166 27 Prague 6, Czech Republic.|Czech Institue of Informatics, Robotics, and Cybernetics, CTU in Prague, Jugoslávských partyzánů 1580/3, CZ-160 00 Prague 6, Czech Republic.
authors.forename: Olga
authors.initials: O
authors.lastname: Stepankova
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic.
authors.forename: Zbynek
authors.initials: Z
authors.lastname: Heger
authors.affiliation: Department of Chemistry and Biochemistry, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic.|Central European Institute of Technology, Brno University of Technology, Technicka 3058/10, CZ-616 00 Brno, Czech Republic.
authors.forename: Vojtech
authors.initials: V
authors.lastname: Adam
mesh_terms: D000818:Animals; D003300:Copper / Q000032:analysis*; D004195:Disease Models, Animal; D006652:Histological Techniques / Q000379:methods*; D007091:Image Processing, Computer-Assisted / Q000379:methods*; D013058:Mass Spectrometry / Q000379:methods*; D008545:Melanoma / Q000473:pathology*; D010865:Pilot Projects; D013552:Swine; D015032:Zinc / Q000032:analysis*
chemical_list: D003300:Copper; D015032:Zinc