title: Proliferation-Related Activity in Endothelial Cells Is Enhanced by Micropower Plasma.
abstract: Nonthermal plasma has received a lot of attention as a medical treatment technique in recent years. It can easily create various reactive chemical species (ROS) and is harmless to living body. Although plasma at gas-liquid interface has a potential for a biomedical application, the interactions between the gas-liquid plasma and living cells remain unclear. Here, we show characteristics of a micropower plasma with 0.018 W of the power input, generated at gas-liquid interface. We also provide the evidence of plasma-induced enhancement in proliferation activity of endothelial cells. The plasma produced H2O2, HNO2, and HNO3in phosphate buffered saline containing Mg++and Ca++(PBS(+)), and their concentration increased linearly during 600-second discharge. The value of pH in PBS(+) against the plasma discharge time was stable at about 7.0. Temperature in PBS(+) rose monotonically, and its rise was up to 0.8°C at the bottom of a cell-cultured dish by the plasma discharge for 600 s. Short-time treatment of the plasma enhanced proliferation activity of endothelial cells. In contrast, the treatment of H2O2does not enhance the cell proliferation. Thus, the ROS production and the nuclear factor-kappa B (NF-κB) activation due to the plasma treatment might be related to enhancement of the cell proliferation. Our results may potentially provide the basis for developing the biomedical applications using the gas-liquid plasma.
authors.affiliation: Graduate School of Engineering, Tohoku University, 6-6-01 Aramaki-Aoba, Aoba, Sendai 980-8579, Japan.
authors.forename: Kotaro
authors.initials: K
authors.lastname: Suzuki
authors.affiliation: Institute of Fluid Science, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.
authors.forename: Daisuke
authors.identifier: 0000-0002-2948-1210
authors.initials: D
authors.lastname: Yoshino
mesh_terms: D049109:Cell Proliferation / Q000187:drug effects*; D061307:Human Umbilical Vein Endothelial Cells / Q000166:cytology / Q000378:metabolism*; D006801:Humans; D058626:Plasma Gases / Q000494:pharmacology*
chemical_list: D058626:Plasma Gases