title: Improvement of glucoamylase production for raw-starch digestion in Aspergillus niger F-01 by maltose stearic acid ester.
abstract: OBJECTIVE
  To investigate the role of maltose stearic acid ester (MSAE) for improving raw-starch-digesting glucoamylase (RSDG) production by Aspergillus niger F-01 and its regulatory mechanism.
  
  
  RESULTS
  RSDG activity was enhanced 8.3-fold by adding 5 g MSAE to 1 l basal medium at the beginning of the culture. RSDG biosynthesis nearly ceased by adding 100 μg actinomycin D/ml or 50 μg cycloheximide/ml in a 48 h culture in the basal medium. RSDG biosynthesis was restarted by adding MSAE in the culture repressed by actinomycin D. No revival of RSDG synthesis was observed by adding MSAE in the culture repressed by cycloheximide.
  
  
  CONCLUSIONS
  MSAE improved RSDG production in A. niger by inducing the transcription of RSDG.
authors.affiliation: College of Agriculture, Hainan University, Haikou, 570228, People's Republic of China.|Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People's Republic of China.
authors.forename: Haiyan
authors.identifier: http://orcid.org/0000-0002-1253-5147
authors.initials: H
authors.lastname: Sun
authors.affiliation: College of Agriculture, Hainan University, Haikou, 570228, People's Republic of China. mingpeng2000@yeah.net.|Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, People's Republic of China. mingpeng2000@yeah.net.
authors.forename: Ming
authors.initials: M
authors.lastname: Peng
mesh_terms: D001234:Aspergillus niger / Q000201:enzymology*; D050260:Carbohydrate Metabolism; D004952:Esters / Q000378:metabolism; D005087:Glucan 1,4-alpha-Glucosidase / Q000096:biosynthesis*; D007218:Industrial Microbiology; D008320:Maltose / Q000378:metabolism*; D013213:Starch / Q000378:metabolism*; D013229:Stearic Acids / Q000378:metabolism*
chemical_list: D004952:Esters; D013229:Stearic Acids; D008320:Maltose; D013213:Starch; D005087:Glucan 1,4-alpha-Glucosidase
keywords: Aspergillus niger; Glucoamylase; Maltose stearic acid ester; Raw-starch-digesting-glucoamylase; Transcription; Translation