title: Tinospora cordifolia extract attenuates cadmium-induced biochemical and histological alterations in the heart of male Wistar rats.
abstract: Persistence of cadmium (Cd) in the environment causes serious ecological problems. Tinospora cordifolia is a medicinal herb used in Ayurveda for treating various metabolic disorders and toxic conditions. The present study investigates the protective effect of T. cordifolia stem methanolic extract (TCME) on a heavy metal, Cd-induced cardiotoxicity in male Wistar rats. Male albino Wistar rats were divided into four groups (n=6). The animals after treatment for 28days with Cd and TCME were analysed for biochemical and histological changes in the serum and heart tissues. Cd induced lipid peroxidation and protein carbonylation was significantly reduced by TCME. TCME also reduced the histological alterations induced by Cd treatment in the heart tissues with diminished loss of myocardial fibers. Administration of TCME effectively prevented the altered levels of serum marker enzymes (creatine kinase and lactate dehydrogenase), antioxidants, such as superoxide dismutase, catalase, glutathione, glutathione peroxidase and glutathione-S-transferase, and glycoproteins contents such as hexose, hexoseamine, fucose, and sialic acid by Cd intoxication. TCME also offered protection against the change in levels of Na+K+ATPase, Mg2+ATPase and Ca2+ATPase activities against Cd toxicity. The study suggests TCME as a potent cardioprotective agent against Cd induced toxicity.
authors.affiliation: Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors.forename: Lohanathan Bharathi
authors.initials: LB
authors.lastname: Priya
authors.affiliation: Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India; DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors.forename: Rathinasamy
authors.initials: R
authors.lastname: Baskaran
authors.affiliation: Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors.forename: Pitchai
authors.initials: P
authors.lastname: Elangovan
authors.affiliation: Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India.
authors.forename: Velumani
authors.initials: V
authors.lastname: Dhivya
authors.affiliation: Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; Graduate Institute of Chinese Medical Science, China Medical University, Taichung, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan.
authors.forename: Chih-Yang
authors.initials: CY
authors.lastname: Huang
authors.affiliation: Translational Research Laboratory, Department of Biotechnology, School of Biotechnology and Genetic Engineering, Bharathiar University, Coimbatore 641046, Tamil Nadu, India; DRDO-BU Centre for Life Sciences, Bharathiar University, Coimbatore 641046, Tamil Nadu, India; Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan; Department of Health and Nutrition Biotechnology, Asia University, Taichung, Taiwan. Electronic address: vvijayapadma@rediffmail.com.
authors.forename: Viswanadha Vijaya
authors.initials: VV
authors.lastname: Padma
mesh_terms: D000818:Animals; D002104:Cadmium / Q000633:toxicity*; D002316:Cardiotonic Agents / Q000302:isolation & purification / Q000494:pharmacology*; D006321:Heart / Q000187:drug effects; D008297:Male; D009206:Myocardium / Q000737:chemistry / Q000378:metabolism* / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects / Q000502:physiology*; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar; D031610:Tinospora
chemical_list: D002316:Cardiotonic Agents; D010936:Plant Extracts; D002104:Cadmium
keywords: Antioxidant enzymes; Cadmium; Heart; Oxidative stress; Tinospora cordifolia