title: A Single-Step Enrichment Medium for Nonchromogenic Isolation of Healthy and Cold-Injured Salmonella spp. from Fresh Vegetables.
abstract: Culture-based detection of nontyphoidal Salmonella spp. in foods requires at least four working days; therefore, new detection methods that shorten the test time are needed. In this study, we developed a novel single-step Salmonella enrichment broth, SSE-1, and compared its detection capability with that of commercial single-step ONE broth-Salmonella (OBS) medium and a conventional two-step enrichment method using buffered peptone water and Rappaport-Vassiliadis soy broth (BPW-RVS). Minimally processed lettuce samples were artificially inoculated with low levels of healthy and cold-injured Salmonella Enteritidis (100or 101colony-forming unit/25 g), incubated in OBS, BPW-RVS, and SSE-1 broths, and streaked on xylose lysine deoxycholate (XLD) agar. Salmonella recoverability was significantly higher in BPW-RVS (79.2%) and SSE-1 (83.3%) compared to OBS (39.3%) (p < 0.05). Our data suggest that the SSE-1 single-step enrichment broth could completely replace two-step enrichment with reduced enrichment time from 48 to 24 h, performing better than commercial single-step enrichment medium in the conventional nonchromogenic Salmonella detection, thus saving time, labor, and cost.
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Hong-Seok
authors.initials: HS
authors.lastname: Kim
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Dasom
authors.initials: D
authors.lastname: Choi
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Il-Byeong
authors.initials: IB
authors.lastname: Kang
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Dong-Hyeon
authors.initials: DH
authors.lastname: Kim
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Jin-Hyeok
authors.initials: JH
authors.lastname: Yim
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Young-Ji
authors.initials: YJ
authors.lastname: Kim
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Jung-Whan
authors.initials: JW
authors.lastname: Chon
authors.affiliation: 2 Department of Food Science and Biotechnology, School of Bioconvergence Science and Technology, Kangwon National University , Chuncheon, Gangwon, South Korea .
authors.forename: Deog-Hwan
authors.initials: DH
authors.lastname: Oh
authors.affiliation: 1 Center for One Health, College of Veterinary Medicine, Konkuk University , Seoul, South Korea .
authors.forename: Kun-Ho
authors.initials: KH
authors.lastname: Seo
mesh_terms: D015169:Colony Count, Microbial; D003470:Culture Media / Q000737:chemistry*; D005506:Food Contamination / Q000032:analysis*; D005516:Food Microbiology / Q000379:methods*; D018545:Lactuca / Q000382:microbiology; D012477:Salmonella enteritidis / Q000254:growth & development / Q000302:isolation & purification*; D014675:Vegetables / Q000382:microbiology*
chemical_list: D003470:Culture Media
keywords: Salmonella; foodborne disease; rapid detection; vegetables