title: Evaluation of sodium arsenite exposure on reproductive competence in pregnant and postlactational dams and their offspring.
abstract: We investigated arsenite exposure on the reproductive axis of dams (during pregnancy and at cyclicity resumption) and their offspring. Pregnant rats were exposed to 5 (A5) or 50ppm (A50) of sodium arsenite in drinking water from gestational day 1 (GD1) until sacrifice at GD18 or two months postpartum. Offspring were exposed to the same treatment as their mothers from weaning to adulthood. A50-pregnant rats gained less weight, showed increased testosterone and estradiol but pregnancy was unaffected. After lactation, arsenic-exposed dams presented compromised cyclicity, decreased estradiol, increased follicle-stimulating hormone (FSH), less preovulatory follicles and presence of ovarian cysts, suggesting impaired reproduction. A50-offspring presented lower body weight; A50-female-offspring showed elevated gonadotropin releasing hormone (GnRH), FSH and testosterone, while A50-males showed diminished GnRH/FSH, but normal testosterone. We conclude that arsenite at the present exposure levels did not compromise pregnancy outcome while it negatively affected reproductive physiology in postpartum dams and their offspring.
authors.affiliation: Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.
authors.forename: Nadia Soledad
authors.initials: NS
authors.lastname: Bourguignon
authors.affiliation: Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.
authors.forename: María Marta
authors.initials: MM
authors.lastname: Bonaventura
authors.affiliation: Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.
authors.forename: Diego
authors.initials: D
authors.lastname: Rodríguez
authors.affiliation: Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina.
authors.forename: Marianne
authors.initials: M
authors.lastname: Bizzozzero
authors.affiliation: Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
authors.forename: Clara
authors.initials: C
authors.lastname: Ventura
authors.affiliation: Laboratorio de Radioisótopos, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
authors.forename: Mariel
authors.initials: M
authors.lastname: Nuñez
authors.affiliation: Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina. Electronic address: vlux@ibyme.conicet.gov.ar.
authors.forename: Victoria Adela
authors.initials: VA
authors.lastname: Lux-Lantos
authors.affiliation: Laboratorio de Neuroendocrinología, Instituto de Biología y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina; Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires (UBA), Argentina.
authors.forename: Carlos
authors.initials: C
authors.lastname: Libertun
mesh_terms: D000311:Adrenal Glands / Q000187:drug effects / Q000378:metabolism; D000818:Animals; D001151:Arsenic / Q000378:metabolism; D018053:Arsenites / Q000633:toxicity*; D005260:Female; D006728:Hormones / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D007774:Lactation; D008099:Liver / Q000378:metabolism; D008297:Male; D008431:Maternal-Fetal Exchange; D010053:Ovary / Q000187:drug effects / Q000378:metabolism; D010902:Pituitary Gland / Q000187:drug effects / Q000378:metabolism; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects; D051381:Rats; D017207:Rats, Sprague-Dawley; D012098:Reproduction / Q000187:drug effects; D012741:Sexual Maturation / Q000187:drug effects; D017670:Sodium Compounds / Q000633:toxicity*
chemical_list: D018053:Arsenites; D006728:Hormones; D017670:Sodium Compounds; C017947:sodium arsenite; D001151:Arsenic
keywords: Hormones; Offspring; Oxidative stress; Postlactational estrous cycles; Pregnancy; Sodium arsenite