title: The Complete Maternally and Paternally Inherited Mitochondrial Genomes of a Freshwater Mussel Potamilus alatus (Bivalvia: Unionidae).
abstract: Doubly uniparental inheritance (DUI) of mitochondrial DNA, found only in some bivalve families and characterized by the existence of gender-associated mtDNA lineages that are inherited through males (M-type) or females (F-type), is one of the very few exceptions to the general rule of strict maternal mtDNA inheritance in animals. M-type sequences are often undetected and hence still underrepresented in the GenBank, which hinders the progress of the understanding of the DUI phenomenon. We have sequenced and analyzed the complete M and F mitogenomes of a freshwater mussel, Potamilus alatus. The M-type was 493 bp longer (M = 16 560, F = 16 067 bp). Gene contents, order and the distribution of genes between L and H strands were typical for unionid mussels. Candidates for the two ORFan genes (forf and morf) were found in respective mitogenomes. Both mitogenomes had a very similar A+T bias: F = 61% and M = 62.2%. The M mitogenome-specific cox2 extension (144 bp) is much shorter than in other sequenced unionid mitogenomes (531-576 bp), which might be characteristic for the Potamilus genus. The overall topology of the phylogenetic tree is in very good agreement with the currently accepted phylogenetic relationships within the Unionidae: both studied sequences were placed within the Ambleminae subfamily clusters in the corresponding M and F clades.
authors.affiliation: Wuxi Fishery College, Nanjing Agriculture University, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes-Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.|Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Hai B
authors.initials: HB
authors.lastname: Wen
authors.affiliation: Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes-Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Zhe M
authors.initials: ZM
authors.lastname: Cao
authors.affiliation: Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Hua
authors.affiliation: Wuxi Fishery College, Nanjing Agriculture University, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes-Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.|Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Pao
authors.initials: P
authors.lastname: Xu
authors.affiliation: Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes-Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.|Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Xue Y
authors.initials: XY
authors.lastname: Ma
authors.affiliation: Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes-Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.|Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Wu
authors.initials: W
authors.lastname: Jin
authors.affiliation: Wuxi Fishery College, Nanjing Agriculture University, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes-Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.|Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Xin H
authors.initials: XH
authors.lastname: Yuan
authors.affiliation: Wuxi Fishery College, Nanjing Agriculture University, Jiangsu, China.|Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes-Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.|Sino-US Cooperative Laboratory for Germplasm Conservation and Utilization of Freshwater Mollusks, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Jiangsu, China.
authors.forename: Ruo B
authors.initials: RB
authors.lastname: Gu
mesh_terms: D000818:Animals; D001482:Base Composition; D005260:Female; D023061:Gene Order; D059646:Genome Size; D054629:Genome, Mitochondrial; D023281:Genomics; D008297:Male; D000072741:Maternal Inheritance; D016366:Open Reading Frames; D000072740:Paternal Inheritance; D010802:Phylogeny; D017422:Sequence Analysis, DNA; D049876:Unionidae / Q000145:classification / Q000235:genetics*