title: [Changes in the receptor activity of β2-adrenoreceptors of human T-lymphocytes under the effect of β2-agonists].
abstract: Changes in the activity of β2-adrenergic receptors of human T-lymphocytes under the effect of salbutamol (a short-acting β2-agonist) have been evaluated with a new modified radioligand method utilizing [^(125)I]cyanopindolol and a specific ligand ICI 118551. In healthy volunteers, the receptor activity decreased after 30 min upon the inhalation of salbutamol and restored to the initial level after 2 h. At the same time, there were changes in the transcription level of the ADRB2 gene, which encodes the protein component of the β2-adrenoreceptor. The dynamics of β2-adrenergic receptor activity of T-lymphocytes after salbutamol treatment in patients with cardiorespiratory pathology significantly differed from that in healthy volunteers.
authors.affiliation: Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, 127473 Russia.
authors.forename: O Y
authors.initials: OY
authors.lastname: Agapova
authors.affiliation: Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997 Russia.|sur@ibch.ru.
authors.forename: Y S
authors.initials: YS
authors.lastname: Skoblov
authors.affiliation: Russian Cardiology Research and Production Complex, Moscow, 121552 Russia.
authors.forename: G A
authors.initials: GA
authors.lastname: Tkachev
authors.affiliation: Russian Cardiology Research and Production Complex, Moscow, 121552 Russia.
authors.forename: N A
authors.initials: NA
authors.lastname: Mironova
authors.affiliation: Russian Cardiology Research and Production Complex, Moscow, 121552 Russia.
authors.forename: S P
authors.initials: SP
authors.lastname: Golitsyn
authors.affiliation: Russian Cardiology Research and Production Complex, Moscow, 121552 Russia.
authors.forename: V P
authors.initials: VP
authors.lastname: Masenko
authors.affiliation: Russian Cardiology Research and Production Complex, Moscow, 121552 Russia.
authors.forename: I E
authors.initials: IE
authors.lastname: Chazova
authors.affiliation: Moscow State University of Medicine and Dentistry, Laboratory of Pulmonology, Moscow, 127473 Russia.
authors.forename: K A
authors.initials: KA
authors.lastname: Zykov
mesh_terms: D058666:Adrenergic beta-2 Receptor Agonists / Q000008:administration & dosage*; D000368:Aged; D000369:Aged, 80 and over; D000420:Albuterol / Q000008:administration & dosage*; D005260:Female; D006801:Humans; D006973:Hypertension / Q000188:drug therapy / Q000378:metabolism; D008297:Male; D008875:Middle Aged; D018343:Receptors, Adrenergic, beta-2 / Q000378:metabolism*; D011948:Receptors, Antigen, T-Cell / Q000378:metabolism*; D012140:Respiratory Tract Diseases / Q000188:drug therapy / Q000378:metabolism; D013601:T-Lymphocytes / Q000378:metabolism*
chemical_list: D058666:Adrenergic beta-2 Receptor Agonists; D018343:Receptors, Adrenergic, beta-2; D011948:Receptors, Antigen, T-Cell; D000420:Albuterol
keywords: ADRB2; T-lymphocytes; activity of β2-adrenoreceptors; gene expression; radioligand binding assay