title: Genetic Variation in FABP4 and Evaluation of Its Effects on Beef Cattle Fat Content.
abstract: FABP4 is a protein primarily expressed in adipocytes and macrophages that plays a key role in fatty acid trafficking and lipid hydrolysis. FABP4 gene polymorphisms have been associated with meat quality traits in cattle, mostly in Asian breeds under feedlot conditions. The objectives of this work were to characterize FABP4 genetic variation in several worldwide cattle breeds and evaluate possible genotype effects on fat content in a pasture-fed crossbred (Angus-Hereford-Limousin) population. We re-sequenced 43 unrelated animals from nine cattle breeds (Angus, Brahman, Creole, Hereford, Holstein, Limousin, Nelore, Shorthorn, and Wagyu) and obtained 22 single nucleotide polymorphisms (SNPs) over 3,164 bp, including four novel polymorphisms. Haplotypes and linkage disequilibrium analyses showed a high variability. Five SNPs were selected to perform validation and association studies in our crossbred population. Four SNPs showed well-balanced allele frequencies (minor frequency > 0.159), and three showed no significant deviations from Hardy-Weinberg proportions. SNPs showed significant effects on backfat thickness and fatty acid composition (P < 0.05). The protein structure of one of the missense SNPs was analyzed to elucidate its possible effect on fat content in our studied population. Our results revealed a possible blockage of the fatty acid binding site by the missense mutation.
authors.affiliation: a IGEVET - Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP , La Plata , Argentina.
authors.forename: Daniel E
authors.initials: DE
authors.lastname: Goszczynski
authors.affiliation: b Unidad Integrada INTA Balcarce, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP) , Balcarce , Argentina.
authors.forename: Juliana
authors.initials: J
authors.lastname: Papaleo-Mazzucco
authors.affiliation: a IGEVET - Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP , La Plata , Argentina.
authors.forename: María V
authors.initials: MV
authors.lastname: Ripoli
authors.affiliation: b Unidad Integrada INTA Balcarce, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP) , Balcarce , Argentina.
authors.forename: Edgardo L
authors.initials: EL
authors.lastname: Villarreal
authors.affiliation: a IGEVET - Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP , La Plata , Argentina.
authors.forename: Andrés
authors.initials: A
authors.lastname: Rogberg-Muñoz
authors.affiliation: b Unidad Integrada INTA Balcarce, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP) , Balcarce , Argentina.
authors.forename: Carlos A
authors.initials: CA
authors.lastname: Mezzadra
authors.affiliation: b Unidad Integrada INTA Balcarce, Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata (UNMdP) , Balcarce , Argentina.
authors.forename: Lilia M
authors.initials: LM
authors.lastname: Melucci
authors.affiliation: a IGEVET - Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout" (UNLP-CONICET LA PLATA), Facultad de Ciencias Veterinarias UNLP , La Plata , Argentina.
authors.forename: Guillermo
authors.initials: G
authors.lastname: Giovambattista
mesh_terms: D000273:Adipose Tissue / Q000502:physiology*; D000818:Animals; D002417:Cattle / Q000235:genetics*; D050556:Fatty Acid-Binding Proteins / Q000235:genetics*; D005260:Female; D015810:Linkage Disequilibrium; D008297:Male; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D000069466:Red Meat / Q000032:analysis* / Q000592:standards
chemical_list: D050556:Fatty Acid-Binding Proteins
keywords: ALBP; SNPs; beef; fatty acid; meat quality