title: A disturbance of intestinal epithelial cell population and kinetics in APC1638T mice.
abstract: The adenomatous polyposis coli (APC) is a multifunctional protein as well as a tumor suppressor. To determine the functions of the C-terminal domain of APC, we explored APC1638T/1638T(APC1638T) mice that express a truncated APC lacking the C-terminal domain. The APC1638T mice were tumor free and exhibited growth retardation. In the present study, we compared small intestinal crypt-villus cells homeostasis in APC+/+(WT) mice and APC1638T mice. The body weight of APC1638T mice was significantly smaller than that of WT mice at all ages. The length of small intestine of APC1638T mice was significantly shorter than that of WT mice. The crypt-villus axis was significantly elongated, and the number of intestinal epithelial cells also increased in APC1638T mice compared with those in WT mice. However, the number of intestinal epithelial cells per 100 µm of villi was not different between WT and APC1638T mice. Migration and proliferation of intestinal epithelial cells in APC1638T mice were faster than that in WT mice. The population of Goblet cells, Paneth cells, and enteroendocrine cells was significantly altered in APC1638T mice. These results indicate that C-terminal domain of APC has a role in the regulation of intestinal epithelium homeostasis.
authors.affiliation: Department of Anatomy, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1194, Japan.
authors.forename: Tuya
authors.initials: T
authors.lastname: Wang
authors.affiliation: Department of Pathology I, Fujita Health University School of Medicine, Toyoake, 470-1192, Japan.
authors.forename: Takanori
authors.initials: T
authors.lastname: Onouchi
authors.affiliation: Department of Anatomy, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1194, Japan.
authors.forename: Nami O
authors.initials: NO
authors.lastname: Yamada
authors.affiliation: Department of Anatomy, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1194, Japan.
authors.forename: Shuji
authors.initials: S
authors.lastname: Matsuda
authors.affiliation: Department of Anatomy, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, 501-1194, Japan. tsenda@gifu-u.ac.jp.
authors.forename: Takao
authors.initials: T
authors.lastname: Senda
mesh_terms: D025601:Adenomatous Polyposis Coli Protein / Q000235:genetics* / Q000378:metabolism; D000818:Animals; D001483:Base Sequence; D049628:Body Size; D002452:Cell Count; D002465:Cell Movement; D049109:Cell Proliferation; D019858:Enteroendocrine Cells / Q000378:metabolism / Q000473:pathology*; D005260:Female; D015870:Gene Expression; D020397:Goblet Cells / Q000378:metabolism / Q000473:pathology*; D007413:Intestinal Mucosa / Q000378:metabolism / Q000473:pathology*; D007421:Intestine, Small / Q000378:metabolism / Q000473:pathology*; D008297:Male; D051379:Mice; D008822:Mice, Transgenic; D019879:Paneth Cells / Q000378:metabolism / Q000473:pathology*; D000072417:Protein Domains; D017384:Sequence Deletion
chemical_list: D025601:Adenomatous Polyposis Coli Protein; C000613192:adenomatous polyposis coli protein, mouse
keywords: APC 1638T/1638T mice; Adenomatous polyposis coli; Enteroendocrine cell; Goblet cell; Intestinal epithelial cells; Paneth cell