title: Poly(glycerol) Used for Constructing Mixed Polymeric Micelles as T1MRI Contrast Agent for Tumor-Targeted Imaging.
abstract: There was much interest in the development of nanoscale delivery vehicles based on polymeric micelles to realize the diagnostic and therapeutic applications in biomedicine. Here, with the purpose of constructing a micellar magnetic resonance imaging (MRI) contrast agent (CA) with well biocompatibility and targeting specificity, two types of amphiphilic diblock polymers, mPEG-PG(DOTA(Gd))-b-PCL and FA-PEG-b-PCL, were synthesized to form mixed micelles by coassembly. The nanostructure of the resulting micellar system consisted of poly(caprolactone) (PCL) as core and poly(glycerol) (PG) and poly(ethylene glycol) (PEG) as shell, simultaneously modified with DOTA(Gd) chelates and folic acid (FA), which afforded functions of MRI contrast enhancement and tumor targeting. The mixed micelles in aqueous solution presented a hydrodynamic diameter of about 85 nm. Additionally, this mixed micelles exhibited higher r1relaxivity (14.01 mM-1S1-) compared with commercial Magnevist (3.95 mM-1S1-) and showed negligible cytotoxicity estimated by WST assay. In vitro and in vivo MRI experiments revealed excellent targeting specificity to tumor cells and tissue. Furthermore, considerably enhanced signal intensity and prominent positive contrast effect were achieved at tumor region after tumor-bearing mice were intravenously injected with the mixed micelles. These preliminary results indicated the potential of the mixed micelle as T1MRI CA for tumor-targeted imaging.
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.|School of Materials Science and Engineering, Nanjing University of Science and Technology , Nanjing 210094, China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Min
authors.identifier: 0000-0003-4779-8489
authors.initials: M
authors.lastname: Liu
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Kunchi
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Guangyue
authors.initials: G
authors.lastname: Zu
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Ye
authors.initials: Y
authors.lastname: Kuang
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Tong
authors.affiliation: School of Materials Science and Engineering, Nanjing University of Science and Technology , Nanjing 210094, China.
authors.forename: Dangsheng
authors.initials: D
authors.lastname: Xiong
authors.affiliation: Key Laboratory of Nano-Bio Interface, Division of Nanobiomedicine, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences , Suzhou 215123, China.
authors.forename: Renjun
authors.initials: R
authors.lastname: Pei
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D003287:Contrast Media / Q000378:metabolism*; D005260:Female; D005492:Folic Acid / Q000378:metabolism; D005990:Glycerol / Q000737:chemistry*; D006801:Humans; D007624:KB Cells; D008279:Magnetic Resonance Imaging / Q000379:methods*; D051379:Mice; D008819:Mice, Nude; D008823:Micelles; D009062:Mouth Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism / Q000473:pathology; D011108:Polymers / Q000008:administration & dosage* / Q000737:chemistry; D023041:Xenograft Model Antitumor Assays
chemical_list: D003287:Contrast Media; D008823:Micelles; D011108:Polymers; D005492:Folic Acid; D005990:Glycerol