title: Enrichment of total steroidal saponins from the extracts of Trillium tschonoskii Maxim by macroporous resin and the simultaneous determination of eight steroidal saponins in the final product by HPLC.
abstract: An effective and simple method was established for the separation and enrichment of steroidal saponins from Trillium tschonoskii Maxim. The adsorption and desorption properties of seven macroporous resins were investigated. Among the tested resins, AB-8 resin showed the best adsorption and desorption capacities. The adsorption of steroidal saponins on AB-8 at 25°C was quite consistent with both the Freundlich isotherm model and the pseudo-second-order kinetics model. By optimizing the dynamic adsorption and desorption parameters, the content of steroidal saponins increased from 5.20% in the crude extracts to 51.93% in the final product, with a recovery yield of 86.67%. Furthermore, by scale-up separation, the concentration and recovery of total steroidal saponins were 43.8 and 85.5%, respectively, which suggested that AB-8 resin had great industrial and pharmaceutical potential because of its high efficiency and cost-effectiveness. In addition, a high-performance liquid chromatography method for the simultaneous determination of eight steroidal saponins was established for the first time, which was employed to qualitatively and quantitatively analyze the final product. Based on the methodological validation results, the high-performance liquid chromatography method can be widely applied to the quality control of steroidal saponins from Trillium tschonoskii Maxim due to its excellent accuracy, stability, and repeatability.
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Yulan
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Department of Pharmacognosy, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Xin
authors.initials: X
authors.lastname: Gao
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Fu
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Pengqi
authors.initials: P
authors.lastname: Guo
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Xinya
authors.initials: X
authors.lastname: Xu
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Ting
authors.initials: T
authors.lastname: Zhang
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Yanhui
authors.initials: Y
authors.lastname: Ge
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Bilin
authors.initials: B
authors.lastname: Zhang
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Mingchan
authors.initials: M
authors.lastname: Wang
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Aiguo
authors.initials: A
authors.lastname: Zeng
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Zhimin
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Pharmacutical Analysis, School of Pharmacy, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
authors.forename: Chun
authors.initials: C
authors.lastname: Chang
mesh_terms: D000327:Adsorption; D002851:Chromatography, High Pressure Liquid; D010936:Plant Extracts / Q000737:chemistry*; D012117:Resins, Synthetic; D012503:Saponins / Q000302:isolation & purification*; D031446:Trillium / Q000737:chemistry*
chemical_list: D010936:Plant Extracts; D012117:Resins, Synthetic; D012503:Saponins
keywords: AB-8 macroporous resin; Trillium tschonoskii Maxim; total saponins