title: Identification and Transcript Analysis of the TCP Transcription Factors in the Diploid Woodland StrawberryFragaria vesca.
abstract: Plant-specific TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (TCP) transcription factors play versatile functions in multiple processes of plant growth and development. However, no systematic study has been performed in strawberry. In this study, 19FvTCPgenes were identified in the diploid woodland strawberry (Fragaria vesca) accession Heilongjiang-3. Phylogenetic analysis suggested that theFvTCPgenes were classified into two main classes, with the second class further divided into two subclasses, which was supported by the exon-intron organizations and the conserved motif structures. Promoter analysis revealed variouscis-acting elements related to growth and development, hormone and/or stress responses. We analyzedFvTCPgene transcript accumulation patterns in different tissues and fruit developmental stages. Among them, 12FvTCPgenes exhibited distinct tissue-specific transcript accumulation patterns. ElevenFvTCPgenes were down-regulated in different fruit developmental stages, while fiveFvTCPgenes were up-regulated. Transcripts ofFvTCPgenes also varied with different subcultural propagation periods and were induced by hormone treatments and biotic and abiotic stresses. Subcellular localization analysis showed that six FvTCP-GFP fusion proteins showed distinct localizations inArabidopsismesophyll protoplasts. Notably, transient over-expression ofFvTCP9in strawberry fruits dramatically affected the expression of a series of genes implicated in fruit development and ripening. Taken together, the present study may provide the basis for functional studies to reveal the role of this gene familyin strawberry growth and development.
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F UniversityShaanxi, China; Key Laboratory of Protected Horticulture Engineering in Northwest China, Ministry of AgricultureShaanxi, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University Shaanxi, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F UniversityShaanxi, China; Key Laboratory of Protected Horticulture Engineering in Northwest China, Ministry of AgricultureShaanxi, China.
authors.forename: Meng-Yuan
authors.initials: MY
authors.lastname: Cui
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University Shaanxi, China.
authors.forename: Yong-Tao
authors.initials: YT
authors.lastname: Han
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F UniversityShaanxi, China; Key Laboratory of Protected Horticulture Engineering in Northwest China, Ministry of AgricultureShaanxi, China.
authors.forename: Kuan
authors.initials: K
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F UniversityShaanxi, China; Key Laboratory of Protected Horticulture Engineering in Northwest China, Ministry of AgricultureShaanxi, China.
authors.forename: Jia-Yue
authors.initials: JY
authors.lastname: Feng
keywords: Strawberry (Fragaria vesca); TCP transcription factors; plant growth and development; subcellular localization; transcript accumulation profiles; transient over-expression