title: Dynamin-2 Stabilizes the HIV-1 Fusion Pore with a Low Oligomeric State.
abstract: One of the key research areas surrounding HIV-1 concerns the regulation of the fusion event that occurs between the virus particle and the host cell during entry. Even if it is universally accepted that the large GTPase dynamin-2 is important during HIV-1 entry, its exact role during the first steps of HIV-1 infection is not well characterized. Here, we have utilized a multidisciplinary approach to study the DNM2 role during fusion of HIV-1 in primary resting CD4 T and TZM-bl cells. We have combined advanced light microscopy and functional cell-based assays to experimentally assess the role of dynamin-2 during these processes. Overall, our data suggest that dynamin-2, as a tetramer, might help to establish hemi-fusion and stabilizes the pore during HIV-1 fusion.
authors.affiliation: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK.
authors.forename: Daniel M
authors.initials: DM
authors.lastname: Jones
authors.affiliation: Wellcome Trust Human Genetics, Cellular Imaging Core, University of Oxford, Oxford OX3 7BN, UK.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Alvarez
authors.affiliation: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK.
authors.forename: Rory
authors.initials: R
authors.lastname: Nolan
authors.affiliation: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK.
authors.forename: Margarita
authors.initials: M
authors.lastname: Ferriz
authors.affiliation: Wellcome Trust Human Genetics, Cellular Imaging Core, University of Oxford, Oxford OX3 7BN, UK.
authors.forename: Raquel
authors.initials: R
authors.lastname: Sainz Urruela
authors.affiliation: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK.
authors.forename: Xènia
authors.initials: X
authors.lastname: Massana-Muñoz
authors.affiliation: The Kennedy Institute of Rheumatology, University of Oxford, Headington, Oxford OX3 7BN, UK.
authors.forename: Hila
authors.initials: H
authors.lastname: Novak-Kotzer
authors.affiliation: The Kennedy Institute of Rheumatology, University of Oxford, Headington, Oxford OX3 7BN, UK.
authors.forename: Michael L
authors.initials: ML
authors.lastname: Dustin
authors.affiliation: Division of Structural Biology, University of Oxford, The Henry Wellcome Building for Genomic Medicine, Headington, Oxford OX3 7BN, UK; Wellcome Trust Human Genetics, Cellular Imaging Core, University of Oxford, Oxford OX3 7BN, UK. Electronic address: spadilla@well.ox.ac.uk.
authors.forename: Sergi
authors.initials: S
authors.lastname: Padilla-Parra
mesh_terms: D015496:CD4-Positive T-Lymphocytes / Q000276:immunology; D002452:Cell Count; D002459:Cell Fusion; D034285:Dynamin II / Q000737:chemistry* / Q000378:metabolism*; D031541:Fluorescence Resonance Energy Transfer; D017930:Genes, Reporter; D057809:HEK293 Cells; D015497:HIV-1 / Q000502:physiology*; D006801:Humans; D006835:Hydrazones / Q000378:metabolism; D007700:Kinetics; D008561:Membrane Fusion; D008954:Models, Biological; D055503:Protein Multimerization; D014771:Virion / Q000378:metabolism; D053586:Virus Internalization
chemical_list: D006835:Hydrazones; C511794:N'-(3,4-dihydroxybenzylidene)-3-hydroxy-2-naphthahydrazide; D034285:Dynamin II
keywords: Dynamin-2; HIV-1 fusion; advanced light imaging; cell-cell fusion; number and brightness