title: [The effects of platelet-rich fibrin extract on MC3T3-E1 cells cultured on the titanium discs].
abstract: Objective:To evaluate the effect of platelet-rich fibrin extract (PRFe) on the adhesion, proliferation and differentiation of MC3T3-E1 cells cultured on the titanium discs.Methods:Samples were divided into experimental group (P) and control group (D). Group P used the α-minimal essential medium (α-MEM) containing PRFe (0.5%), while group D used only the α-MEM. Cell adhesion and cytoskeleton were observed using scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). Methyl thiazolyl tetrazolium (MTT) assay to detect the number of the osteoblasts at 1, 3, 5, 7 d; the activity of alkaline phosphatase (ALP) to detect the differentiation of osteoblast at 1, 3, 5, 7 d; the level of osteogenetic biomarkers core-binding factorα1 (cbfα1) and osteocalcin (OCN) were quantified by quantitative real-time PCR (qRT-PCR) at 3 and 7 d.Results:SEM and LSCM showed that the adhesion and filaments of group P were higher than those of group D at each time point. MTT assay showed that the absorbance were significantly increased in group P (1 d: 0.299±0.002, 3 d: 0.517±0.004, 5 d: 0.810±0.002, 7 d: 1.203±0.011) compared with group D (1 d: 0.198±0.003, 3 d: 0.399±0.002, 5 d: 0.588±0.002, 7 d: 0.897±0.005) at each time points (P<0.05). Furthermore, the ALP activity of group P (1 d: 0.162±0.004, 3 d: 0.289±0.001, 5 d: 0.491±0.006, 7 d: 0.647±0.005) was significantly higher than that of group D (1 d: 0.121±0.003, 3 d: 0.191± 0.006, 5 d: 0.252±0.004, 7 d: 0.365±0.012), (P<0.05). Moreover, the qRT-PCR showed that the Cbfα1 and OCN gene expression in group P (Cfbα1, 3 d: 1.50±0.04, 7 d: 1.94±0.06; OCN, 3 d: 3.37±0.17, 7 d: 3.92± 0.04) were significantly higher than that in group D(Cfbα1, 3 d: 1, 7 d: 1.18±0.13; OCN, 3 d: 1, 7 d: 2.34± 0.09) (P<0.05).Conclusions:PRFe promoted the adhension, proliferation and differentiation of MC3T3-E1 cells on the titanium discs.
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical College, Yantai Shandong 264008, China.
authors.forename: X J
authors.initials: XJ
authors.lastname: Zhang
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical College, Yantai Shandong 264008, China.
authors.forename: S
authors.initials: S
authors.lastname: Xu
authors.affiliation: Department of Dental Implantology, School of Stomatology, Jilin University, Changchun 130021, China.
authors.forename: W Y
authors.initials: WY
authors.lastname: Meng
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical College, Yantai Shandong 264008, China.
authors.forename: H J
authors.initials: HJ
authors.lastname: Xiao
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical College, Yantai Shandong 264008, China.
authors.forename: K
authors.initials: K
authors.lastname: Dong
authors.affiliation: Department of Dental Implantology, Yantai Stomatological Hospital Affiliated to Binzhou Medical College, Yantai Shandong 264008, China.
authors.forename: Z H
authors.initials: ZH
authors.lastname: Liu
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D000818:Animals; D001792:Blood Platelets; D002448:Cell Adhesion; D002454:Cell Differentiation; D002460:Cell Line; D049109:Cell Proliferation; D002478:Cells, Cultured; D003599:Cytoskeleton; D005337:Fibrin; D051379:Mice; D010006:Osteoblasts; D015675:Osteocalcin / Q000378:metabolism; D010012:Osteogenesis; D060888:Real-Time Polymerase Chain Reaction; D013778:Tetrazolium Salts; D014025:Titanium
chemical_list: D013778:Tetrazolium Salts; D015675:Osteocalcin; D005337:Fibrin; D014025:Titanium; D000469:Alkaline Phosphatase