title: CsA Promotes XIST Expression to Regulate Human Trophoblast Cells Proliferation and Invasion Through miR-144/Titin Axis.
abstract: In our previous study, we revealed that Cyclosporin A (CsA) could inhibit miR-144 expression to regulate proliferation and invasion of human trophoblast (HT) cells through miR-144 targeting titin. This partially demonstrated the mechanism by which CsA promotes titin expression to increase the vitality of HT cells. However, the mechanism by which CsA inhibits miR-144 expression remains to be investigated. Recently, the interaction between lncRNA and miRNA has been frequently reported to play major role in several biological processes. In the present study, online tools were used to figure out that X-inactive specific transcript (XIST) could interact with miR-144. XIST and miR-144 reciprocally inhibited each other in HT cells; as exhibited by luciferase reporter gene assays, miR-144 bind to XIST by direct targeting. XIST suppressed miR-144 expression to promote titin expression. As exhibited by the Spearman's correlation analysis, in CsA treated HT cells, miR-144 was inversely correlated with titin and XIST, respectively; XIST was positively correlated with titin. Moreover, CsA could promote the proliferation and invasion of HT cells through XIST and the downstream MAPK and MMPs pathway. Taken together, these findings will shed light to the role and mechanism of CsA/XIST/miR-144/titin in regulating HT cells proliferation and invasion. XIST may serve as a potential therapeutic target in HT in the future. J. Cell. Biochem. 118: 2208-2218, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, Changsha, 410008, P.R. China.|Department of Pharmacy, Changsha Hospital for Maternal and Child Health Care, Changsha, 410007, P.R. China.
authors.forename: Nanhui
authors.initials: N
authors.lastname: Yu
authors.affiliation: Department of Food Science and Engineering, Central South University of Forestry and Technology, Changsha, 410007, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Liang
authors.affiliation: Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, Changsha, 410008, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Zhu
authors.affiliation: Department of Gynaecology, Changsha Hospital for Maternal and Child Health Care, Changsha, 410007, P.R. China.
authors.forename: Hongying
authors.initials: H
authors.lastname: Mo
authors.affiliation: Department of Gastrointestinal Surgery, Xiangya Hospital, Central South University, Changsha, 410008, P.R. China.
authors.forename: Haiping
authors.initials: H
authors.lastname: Pei
mesh_terms: D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000187:drug effects* / Q000235:genetics; D049109:Cell Proliferation / Q000187:drug effects* / Q000235:genetics; D064211:Connectin / Q000235:genetics / Q000378:metabolism*; D016572:Cyclosporine / Q000494:pharmacology*; D006801:Humans; D020782:Matrix Metalloproteinases / Q000235:genetics / Q000378:metabolism; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D020928:Mitogen-Activated Protein Kinases / Q000235:genetics / Q000378:metabolism; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*; D014327:Trophoblasts / Q000166:cytology / Q000187:drug effects* / Q000378:metabolism*
chemical_list: D064211:Connectin; C554293:MIRN144 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C077885:XIST non-coding RNA; D016572:Cyclosporine; D020928:Mitogen-Activated Protein Kinases; D020782:Matrix Metalloproteinases
keywords: CsA; HUMAN TROPHOBLAST CELL; TITIN; XIST; miR-144