title: Functional significance of CD105-positive cells in papillary renal cell carcinoma.
abstract: CD105 was postulated as a renal cell carcinoma (RCC) stem cell marker, and CD133 as a putative RCC progenitor. Hypoxia, a natural microenvironment that prevails in tumors, was also incorporated into the study, especially in terms of the promotion of hypothetical stem-like cell properties.
  
  
  
  Within this study, we verify the existence of CD105+ and CD133+ populations in selected papillary subtype RCC (pRCC) cell lines. Both populations were analyzed for correlation with stem-like cell properties, such as stemness gene expression, and sphere and colony formation. For the preliminary analysis, several RCC cell lines were chosen (786-O, SMKT-R2, Caki-2, 796-P, ACHN, RCC6) and the control was human kidney cancer stem cells (HKCSC) and renal cells of embryonic origin (ASE-5063). Four cell lines were chosen for further investigation: Caki-2 (one of the highest numbers of CD105+ cells; primary origin), ACHN (a low number of CD105+ cells; metastatic origin), HKCSC (putative positive control), and ASE-5063 (additional control).
  
  
  
  In 769-P and RCC6, we could not detect a CD105+ population. Hypoxia variously affects pRCC cell growth, and mainly diminishes the stem-like properties of cells. Furthermore, we could not observe the correlation of CD105 and/or CD133 expression with the enhancement of stem-like properties.
  
  
  
  Based on this analysis, CD105/CD133 cannot be validated as cancer stem cell markers of pRCC cell lines.
authors.affiliation: Department of Oncology with Laboratory of Molecular Oncology, Military Institute of Medicine, Szaserow 128, 04-141, Warsaw, Poland.|School of Molecular Medicine, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Damian
authors.initials: D
authors.lastname: Matak
authors.affiliation: Department of Oncology with Laboratory of Molecular Oncology, Military Institute of Medicine, Szaserow 128, 04-141, Warsaw, Poland.
authors.forename: Klaudia K
authors.initials: KK
authors.lastname: Brodaczewska
authors.affiliation: Department of Oncology with Laboratory of Molecular Oncology, Military Institute of Medicine, Szaserow 128, 04-141, Warsaw, Poland.
authors.forename: Cezary
authors.initials: C
authors.lastname: Szczylik
authors.affiliation: Department of Pathomorphology, Institute of Mother and Child, Warsaw, Poland.
authors.forename: Irena
authors.initials: I
authors.lastname: Koch
authors.affiliation: Department of Oncology with Laboratory of Molecular Oncology, Military Institute of Medicine, Szaserow 128, 04-141, Warsaw, Poland.|Department of Molecular Biology, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Warsaw, Poland.|Current address: Max-Delbrück-Centrum für Molekulare Medizin, Berlin, Germany.
authors.forename: Adam
authors.initials: A
authors.lastname: Myszczyszyn
authors.affiliation: Department of Oncology with Laboratory of Molecular Oncology, Military Institute of Medicine, Szaserow 128, 04-141, Warsaw, Poland.|Faculty of Pharmacy with Laboratory Medicine Division, Medical University of Warsaw, Warsaw, Poland.
authors.forename: Monika
authors.initials: M
authors.lastname: Lipiec
authors.affiliation: Department of Regenerative Medicine, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
authors.forename: Slawomir
authors.initials: S
authors.lastname: Lewicki
authors.affiliation: Department of Oncology with Laboratory of Molecular Oncology, Military Institute of Medicine, Szaserow 128, 04-141, Warsaw, Poland.|Institute of Genetics and Biotechnology, Faculty of Biology, University of Warsaw, Warsaw, Poland.|Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
authors.forename: Lukasz
authors.initials: L
authors.lastname: Szymanski
authors.affiliation: Department of Regenerative Medicine, Military Institute of Hygiene and Epidemiology, Warsaw, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Zdanowski
authors.affiliation: Department of Oncology with Laboratory of Molecular Oncology, Military Institute of Medicine, Szaserow 128, 04-141, Warsaw, Poland. anna.czarnecka@gmail.com.
authors.forename: Anna M
authors.initials: AM
authors.lastname: Czarnecka
mesh_terms: D002291:Carcinoma, Papillary / Q000473:pathology*; D002292:Carcinoma, Renal Cell / Q000473:pathology*; D045744:Cell Line, Tumor; D002469:Cell Separation; D000071063:Endoglin / Q000032:analysis*; D020869:Gene Expression Profiling; D006801:Humans; D007150:Immunohistochemistry; D007680:Kidney Neoplasms / Q000473:pathology*; D014411:Neoplastic Stem Cells / Q000473:pathology*; D060888:Real-Time Polymerase Chain Reaction; D059467:Transcriptome
chemical_list: C064206:ENG protein, human; D000071063:Endoglin
keywords: CD105; Cancer stem cells; Endoglin; Papillary cancer; Renal cell cancer; Tumor initiating cells