title: CD95-Mediated Calcium Signaling.
abstract: Intracellular calcium signals regulate cell function and cell survival by controlling many processes. CD95 engagement results in distinct intracellular calcium signals that control the cell fate, apoptosis, or survival, depending on the ligand (membrane or soluble). Intracellular calcium determination is an exquisite readout to explore the molecular mechanisms elicited by CD95 engagement. The most widely applied methods for studying calcium signaling pathways use fluorescent indicators and imaging methods with fluorescence microscopy. This technical approach, however, requires many precautions that we discuss in this chapter.
authors.affiliation: INSERM U1218, 229 COURS DE L'ARGONNE, CS 61283, 33076, Bordeaux Cedex, France.
authors.forename: Mehdi
authors.initials: M
authors.lastname: Hammadi
authors.affiliation: INSERM U1218, 229 COURS DE L'ARGONNE, CS 61283, 33076, Bordeaux Cedex, France.
authors.forename: Vanessa
authors.initials: V
authors.lastname: Delcroix
authors.affiliation: INSERM U1218, 229 COURS DE L'ARGONNE, CS 61283, 33076, Bordeaux Cedex, France.
authors.forename: Anne-Marie
authors.initials: AM
authors.lastname: Vacher
authors.affiliation: INSERM U1218, 229 COURS DE L'ARGONNE, CS 61283, 33076, Bordeaux Cedex, France.
authors.forename: Thomas
authors.initials: T
authors.lastname: Ducret
authors.affiliation: INSERM U1218, 229 COURS DE L'ARGONNE, CS 61283, 33076, Bordeaux Cedex, France. Pierre.Vacher@inserm.fr.
authors.forename: Pierre
authors.initials: P
authors.lastname: Vacher
mesh_terms: D002118:Calcium / Q000378:metabolism*; D020013:Calcium Signaling; D002460:Cell Line; D053222:Fas Ligand Protein / Q000378:metabolism; D006801:Humans; D018613:Microscopy, Confocal; D008928:Mitochondria / Q000378:metabolism; D057054:Molecular Imaging / Q000379:methods; D011485:Protein Binding; D019014:fas Receptor / Q000378:metabolism*
chemical_list: D053222:Fas Ligand Protein; D019014:fas Receptor; D002118:Calcium
keywords: Confocal microscopy; Fluo2; Fura2; MitoTracker; Mitochondria; Pluronic acid® F-127; Rhod2