title: "In-House" Production of Single Stranded Oligodeoxyribonucleotides.
abstract: The most widely used technique for the production of DNA aptamers/oligonucleotides is chemical synthesis. Despite its effectiveness, this technique cannot be performed "in house", making the user fully dependent on a supplier. In this work, we present a simplified method by which it is possible to enzymatically produce DNA aptamers "in house". This new method uses the rolling circle replication followed by a unique cleavage step using the SchI endonuclease. Potentially, any oligonucleotide can be produced by the enzymatic method proposed in this study. To illustrate, we present the production of three variations of the 31-TBA aptamer, a single stranded DNA which has anticoagulant action.
authors.affiliation: 1 Laboratório de Biologia Molecular Aplicada-LAPLIC, Departamento de Bioquímica, Centro de Biocieências Universidade Federal do Rio Grande do Norte , Natal, Brazil .|2 Programa de Pós-Graduação em Bioquímica, Departamento de Bioquímica, Centro de Biocieências Universidade Federal do Rio Grande do Norte , Natal, Brazil .
authors.forename: Allan R D
authors.initials: AR
authors.lastname: Nunes
authors.affiliation: 2 Programa de Pós-Graduação em Bioquímica, Departamento de Bioquímica, Centro de Biocieências Universidade Federal do Rio Grande do Norte , Natal, Brazil .
authors.forename: Suely F
authors.initials: SF
authors.lastname: Chavante
authors.affiliation: 2 Programa de Pós-Graduação em Bioquímica, Departamento de Bioquímica, Centro de Biocieências Universidade Federal do Rio Grande do Norte , Natal, Brazil .
authors.forename: Hugo A O
authors.initials: HA
authors.lastname: Rocha
authors.affiliation: 1 Laboratório de Biologia Molecular Aplicada-LAPLIC, Departamento de Bioquímica, Centro de Biocieências Universidade Federal do Rio Grande do Norte , Natal, Brazil .|2 Programa de Pós-Graduação em Bioquímica, Departamento de Bioquímica, Centro de Biocieências Universidade Federal do Rio Grande do Norte , Natal, Brazil .
authors.forename: Daniel C F
authors.initials: DC
authors.lastname: Lanza
mesh_terms: D000925:Anticoagulants / Q000138:chemical synthesis / Q000378:metabolism; D052157:Aptamers, Nucleotide / Q000096:biosynthesis* / Q000235:genetics; D001483:Base Sequence; D004262:DNA Restriction Enzymes / Q000378:metabolism; D004277:DNA, Single-Stranded / Q000096:biosynthesis* / Q000235:genetics; D054856:G-Quadruplexes; D006801:Humans; D021141:Nucleic Acid Amplification Techniques; D009838:Oligodeoxyribonucleotides / Q000096:biosynthesis* / Q000235:genetics
chemical_list: D000925:Anticoagulants; D052157:Aptamers, Nucleotide; D004277:DNA, Single-Stranded; D009838:Oligodeoxyribonucleotides; D004262:DNA Restriction Enzymes
keywords: G-quadruplex; anticoagulant aptamer; enzymatic synthesis; oligonucleotide production; rolling circle amplification