title: Combining In Vivo and In Vitro Approaches To Identify Human Odorant Receptors Responsive to Food Odorants.
abstract: Olfactory perception plays an important role in food flavor. Humans have around 400 odorant receptors (ORs), which can be activated by an enormous number of odorants in a combinatorial fashion. To date, only a few odorant receptors have been linked to their respective odorants, due to the difficulties in expressing these receptor proteins in heterologous cell systems. In vivo approaches allow for the analysis of odorant-receptor interactions in their native environment and have the advantage that the complete OR repertoire is simultaneously tested. Once mouse odorant-receptor pairs are defined, one can search for the corresponding human orthologues, which can be validated against the odorants in heterologous cells. Thus, the combination of in vivo and in vitro methods should contribute to the identification of human ORs that recognize odorants of interest, such as key food odorants.
authors.affiliation: Department of Biological Sciences, Diadema Campus , Federal University of São Paulo , São Paulo , Brazil.
authors.forename: Lucia M
authors.initials: LM
authors.lastname: Armelin-Correa
authors.affiliation: Department of Biochemistry , University of São Paulo , São Paulo , Brazil.
authors.forename: Bettina
authors.identifier: http://orcid.org/0000-0002-5877-4784
authors.initials: B
authors.lastname: Malnic
mesh_terms: D000818:Animals; D005504:Food Analysis; D006801:Humans; D051379:Mice; D009812:Odorants / Q000032:analysis*; D055696:Olfactory Perception; D018035:Receptors, Odorant / Q000235:genetics / Q000378:metabolism*; D012903:Smell
chemical_list: D018035:Receptors, Odorant
keywords: key food odorants; odorant receptors; odorants; olfactory sensory neurons; receptor deorphanization