title: Comparative molecular analysis of bacterial species associated with periodontal disease.
abstract: Periodontal disease is an inflammatory disorder affecting the supporting teeth structures, including gingiva, periodontal ligament and alveolar bone, causing loss of connective tissue, reabsorption of alveolar bone and formation of periodontal pockets. The aim of this study is to find a correlation between bacterial growth and periodontal disease. Fifty-seven patients aged between 21 and 65 years, median age 46 years, were enrolled. According to gingival pocket depth, ranging from 3 to 7 mm, patients were divided into two groups: the first (30 patients, 53%) with deep pockets ³ 5 mm and the second (27 patients, 47%) less than 5 mm. The samples taken were processed for microbiological analysis by absolute quantitative real-time Taq-Man technique. Patients affected by periodontal disease were 32 (56%) and patients with gingival bleeding were 35 (61%). This data showed that the presence, the type and the bacterial load in gingival pockets were strongly correlated with gingival depth, periodontal disease and gingival bleeding. Quantitative microbiological analysis is a key point to improve patient compliance, allowing to choose the specific antibiotic treatment. avoiding antibiotic resistance and ensuring the successful outcome of therapy for periodontal disease.
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: V
authors.initials: V
authors.lastname: De Iuliis
authors.affiliation: SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, ASL Lanciano-Vasto-Chieti, Chieti, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Ursi
authors.affiliation: SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, ASL Lanciano-Vasto-Chieti, Chieti, Italy.
authors.forename: L M
authors.initials: LM
authors.lastname: Di Tommaso
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Caruso
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: A
authors.initials: A
authors.lastname: Marino
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: S
authors.initials: S
authors.lastname: D'Ercole
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Caputi
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: B
authors.initials: B
authors.lastname: Sinjari
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: F
authors.initials: F
authors.lastname: Festa
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: M
authors.initials: M
authors.lastname: Macri
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.|SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, ASL Lanciano-Vasto-Chieti, Chieti, Italy.
authors.forename: S
authors.initials: S
authors.lastname: Martinotti
authors.affiliation: SS Annunziata University Hospital, Unit of Clinical Molecular Biology and Predictive Medicine, University of Chieti, ASL Lanciano-Vasto-Chieti, Chieti, Italy.
authors.forename: G
authors.initials: G
authors.lastname: Vitullo
authors.affiliation: Department of Medical, Oral and Biotechnological Sciences, University of Chieti, Chieti, Italy.
authors.forename: E
authors.initials: E
authors.lastname: Toniato
mesh_terms: D000328:Adult; D000368:Aged; D005260:Female; D005884:Gingival Hemorrhage / Q000209:etiology / Q000382:microbiology; D006801:Humans; D008297:Male; D008875:Middle Aged; D010510:Periodontal Diseases / Q000150:complications / Q000382:microbiology*; D010514:Periodontal Pocket / Q000209:etiology / Q000382:microbiology; D016133:Polymerase Chain Reaction; D055815:Young Adult