title: Post-transcriptional regulation of interleukin-10 in peripheral B cells of airway allergy patients.
abstract: The dysfunction of peripheral immune tolerance plays an important role in the pathogenesis of allergic diseases. Recent reports indicate that micro RNA (miR)-98 is associated with the process of aberrant immune responses. This study aims to test a hypothesis that miR-98 is associated with the pathogenesis of airway allergy via interfering with the development of regulatory B cells (Breg). In this study, patients with airway allergy were recruited into this study. The frequency of Bregs was assessed by flow cytometry. The levels of miR-98 in peripheral B cells were determined by RT-qPCR. A cell-culture model of B cells was developed to test the role of miR-98 in the repressing of interleukin (IL)-10 in B cells. The results showed that the levels of IL-10 in peripheral B cells were significantly lower in patients with airway allergy as compared with healthy subjects. High levels of miR-98 (one of the miR-98 members) were detected in peripheral B cells of patients with airway allergy, which was mimicked by stimulating B cells with IL-4. Histone acetyltransferase p300 was involved in the IL-4-induced miR-98 expression. miR-98 mediated the IL-4-inhibited IL-10 expression in B cells. In conclusion, miR-98 affects the expression of IL-10 in B cells and may be a novel therapeutic target for the treatment of allergic diseases.
authors.affiliation: Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510010, China.
authors.forename: Xiang-Qian
authors.initials: XQ
authors.lastname: Luo
authors.affiliation: Department of Cadre Clinic, Chinese PLA General Hospital Beijing 100853, China.
authors.forename: Shao-Bo
authors.initials: SB
authors.lastname: Yang
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Shu-Qi
authors.initials: SQ
authors.lastname: Qiu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of Medicine Shenzhen 518060, China.
authors.forename: Rui-Di
authors.initials: RD
authors.lastname: Xie
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Li-Tao
authors.initials: LT
authors.lastname: Yang
authors.affiliation: Shenzhen Maternity & Child Health Hospital Shenzhen 518052, China.
authors.forename: Yu-Xing
authors.initials: YX
authors.lastname: Ke
authors.affiliation: Shenzhen Maternity & Child Health Hospital Shenzhen 518052, China.
authors.forename: Hong-Xia
authors.initials: HX
authors.lastname: Zhao
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Xiao-Rui
authors.initials: XR
authors.lastname: Geng
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Gui
authors.initials: G
authors.lastname: Yang
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Zhi-Qiang
authors.initials: ZQ
authors.lastname: Liu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Jiang-Qi
authors.initials: JQ
authors.lastname: Liu
authors.affiliation: The Research Center of Allergy & Immunology, Shenzhen University School of MedicineShenzhen 518060, China; Longgang ENT HospitalShenzhen 518116, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Wang
authors.affiliation: Department of Otolaryngology, Guangzhou Women and Children's Medical Center, Guangzhou Medical University Guangzhou 510010, China.
authors.forename: Da-Bo
authors.initials: DB
authors.lastname: Liu
authors.affiliation: Shenzhen Maternity & Child Health Hospital Shenzhen 518052, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
keywords: Allergy; B cell; airway; interleukin-10; micro RNA