title: Promotion effect of sulfite on deoxyosones and 4-methylimidazole in caramel model system.
abstract: In this study, hydrogen-deuterium (H/D) exchange experiment was carried out to reveal the promotion effect of sulfite on the formation of deoxyosones and 4-methylimidazole (4-MeI) in the Maillard reaction. Glucose-ammonium (40mmol/L, pH 7.4 in PBS) model systems with different levels of sulfite were incubated at 110°C for 2h. Alpha-dicarbonyls were detected after derivatization by a high-performance liquid chromatography with a diode array detector (HPLC-DAD). 4-MeI in the Maillard reaction was tested using a high-performance anion exchange chromatography with an electrochemical detector (HPAEC-ED). The H/D exchange ratios of hexose (fructose, glucose and mannose) were tested by HPAEC-MS. Results showed that the aldo-enol transition of enediol was promoted by sulfite, which promoted the formation of deoxyosones and 4-MeI. In addition, the oxidation reaction of enediols was inhibited by the antioxidant sulfite, which caused the inhibition of osones formation in the Maillard reaction.
authors.affiliation: National Engineering Research Center of Seafood, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China.
authors.forename: Xian-Bing
authors.initials: XB
authors.lastname: Xu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China.
authors.forename: Pei
authors.initials: P
authors.lastname: Yu
authors.affiliation: College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China; Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China. Electronic address: shujuanyu8@gmail.com.
authors.forename: Shu-Juan
authors.initials: SJ
authors.lastname: Yu
mesh_terms: D002182:Candy / Q000032:analysis*; D002241:Carbohydrates; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D041961:Deuterium Exchange Measurement / Q000379:methods; D005632:Fructose / Q000032:analysis; D005947:Glucose / Q000032:analysis; D007093:Imidazoles / Q000032:analysis* / Q000378:metabolism*; D015416:Maillard Reaction; D013447:Sulfites / Q000378:metabolism* / Q000494:pharmacology
chemical_list: D002241:Carbohydrates; D007093:Imidazoles; D013447:Sulfites; C569050:caramel coloring; D005632:Fructose; D005947:Glucose; C023439:4-methylimidazole; C025026:sodium sulfite
keywords: 4-Methylimidazole; Alpha-dicarbonyls; Class IV caramel color; Enediol; H/D exchange; Sulfite