title: MiR-218 Induces Neuronal Differentiation of ASCs in a Temporally Sequential Manner with Fibroblast Growth Factor by Regulation of the Wnt Signaling Pathway.
abstract: Differentiation of neural lineages from mesenchymal stem cells has raised the hope of generating functional cells as seed cells for nerve tissue engineering. As important gene regulators, microRNAs (miRNAs) have been speculated to play a vital role in accelerating stem cell differentiation and repairing neuron damage. However, miRNA roles in directing differentiation of stem cells in current protocols are underexplored and the mechanisms of miRNAs as regulators of neuronal differentiation remain ambiguous. In this study, we have determined that miR-218 serves as crucial constituent regulator in neuronal differentiation of adipose stem cells (ASCs) through Wnt signaling pathway based on comprehensive annotation of miRNA sequencing data. Moreover, we have also discovered that miR-218 and Fibroblast Growth Factor-2 (FGF2) modulate neuronal differentiation in a sequential manner. These findings provide additional understanding of the mechanisms regulating stem cell neuronal differentiation as well as a new method for neural lineage differentiation of ASCs.
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu, China.|Medical School, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Feihu
authors.initials: F
authors.lastname: Hu
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Sun
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Xu
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Yanliang
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Xian-Hui
authors.initials: XH
authors.lastname: Meng
authors.affiliation: Medical School, Southeast University, Nanjing, Jiangsu, China.|Jiangsu Key Laboratory of Molecular and Functional Imaging, Department of Radiology, Zhongda Hospital, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Gao-Jun
authors.initials: GJ
authors.lastname: Teng
authors.affiliation: State Key Laboratory of Bioelectronics, Southeast University, Nanjing, Jiangsu, China.
authors.forename: Zhong-Dang
authors.initials: ZD
authors.lastname: Xiao
mesh_terms: D000818:Animals; D002454:Cell Differentiation; D005346:Fibroblast Growth Factors / Q000378:metabolism*; D005786:Gene Expression Regulation; D059630:Mesenchymal Stem Cells / Q000502:physiology*; D035683:MicroRNAs / Q000378:metabolism*; D009474:Neurons / Q000502:physiology*; D017207:Rats, Sprague-Dawley; D060449:Wnt Signaling Pathway
chemical_list: C000628521:MIRN218 microRNA, rat; D035683:MicroRNAs; D005346:Fibroblast Growth Factors