title: Imaging of Cell Shape Alteration and Cell Movement in Drosophila Gastrulation Using DE-cadherin Reporter Transgenic Flies.
abstract: Gastrulation is the first set of morphologically dynamic events that occur during the embryonic development of multicellular animals such as Drosophila. This morphological alteration is also recognized as epithelial to mesenchymal transition (EMT). Dysregulation of EMT is associated with fibrosis and cancer metastasis. There is emerging evidence that EMT is controlled by a number of molecular mechanisms. As such, many key genes that control apical constriction are also known to be important factors in the EMT observed in cancer metastasis. Like EMT during Drosophila gastrulation, epithelial cells can be induced to change their shape and be reprogrammed to redirect cell fate towards various other cell types. Here we provide a robust imaging method of Drosophila gastrulation to assay the initiation of morphogenetic cellular movements and cell fate identification during this stage of embryonic development. Using this method, we identify cell rearrangement at the time of gastrulation and demonstrate the importance of apical constriction during gastrulation using GFP labeled DE-cadherin.
authors.affiliation: Biotechnology and Genetic Engineering Department, Jahangirnagar University.
authors.forename: M Rezaul
authors.initials: MR
authors.lastname: Karim
authors.affiliation: JT Biohistory Research Hall.
authors.forename: Tomohiro
authors.initials: T
authors.lastname: Haruta
authors.affiliation: Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University.
authors.forename: Taro
authors.initials: T
authors.lastname: Matsumoto
authors.affiliation: JT Biohistory Research Hall.
authors.forename: Hiroki
authors.initials: H
authors.lastname: Oda
authors.affiliation: Division of Cell Regeneration and Transplantation, School of Medicine, Nihon University; Graduate School of Life and Medical Sciences, Doshisha University; Institute of Genetics and Animal Breeding of the Polish Academy of Sciences; hiroatanigu@gmail.com.
authors.forename: Hiroaki
authors.initials: H
authors.lastname: Taniguchi
mesh_terms: D000818:Animals; D030801:Animals, Genetically Modified; D015820:Cadherins / Q000235:genetics*; D002465:Cell Movement; D048430:Cell Shape; D065150:Cellular Reprogramming; D004330:Drosophila / Q000196:embryology*; D004847:Epithelial Cells / Q000166:cytology*; D058750:Epithelial-Mesenchymal Transition; D054262:Gastrulation; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics
chemical_list: D015820:Cadherins; D049452:Green Fluorescent Proteins