title: HPV Genotypes distribution in Indian women with and without cervical carcinoma: Implication for HPV vaccination program in Odisha, Eastern India.
abstract: Considering the limited cross protection offered by the current HPV vaccines, understanding the HPV genotype distribution among the different population is essential in predicting the efficacy of current vaccine and devising new vaccine strategy. The present work aimed at investigating the HPV genotypes distribution among women with and without cervical carcinoma in Odisha, Eastern India.
  
  
  
  A total of 607 participants have been enrolled between January 2014 and June 2016. L1-PCR, sequencing, and E6/E7 nested multiplex type- specific PCR were performed for HPV detection and genotyping. Cytological distribution of 440 cases includes invasive cervical carcinoma or ICC (n = 210), inflammatory smear (n = 162), normal cytology (n = 68). Statistical analyses were performed by using SPSS version 20.0 software and MediCal version 14.10.2(7). A p-value of ≤ 0.05 was considered statistically significant.
  
  
  
  The overall prevalence of HPV infection was (359/595) 60.33%. Prevalence of HPV infection was 93.80% (197/210) in invasive cervical cancer (ICC) cases, 54.32% (88/162) in inflammatory smear and 19.11% (13/68) in normal cervical cytology. The most prevalent genotype was HPV16 (87.28%) followed by HPV18 (24.56%) and HPV 51(3.46%). The overall prevalence of single type was 76.58% and highest (78.9%) among ICC cases. The most frequent genotype combination after HPV16 + 18(9.4%) was HPV16 + 66 + 68(2.7%) which was frequently observed in inflammatory cytology. Age > 45years, parity ≥3, low socio-economic condition, rural residential area and post menopause state were significantly associated with HPV infection. Multiple infections did not have a significant association with any of the clinicopathological variables (stage, LN metastasis, cell type) except tumor size ≥ 2cm in ICC cases. The impact of 2v, 4v, and 9v vaccines in preventing cervical cancer in Odisha were 89.99, 91.65, and 92.16% respectively.
  
  
  
  This data would help planning an appropriate strategy for disease monitoring and provides baseline data for post-vaccination surveillance in the region. The nonavalent vaccine would be significant in preventing cervical carcinoma in Odisha. Hence an effective vaccination program based on regional HPV epidemiological profile along with the cervical cancer screening is necessary to reduce the cervical cancer burden in India.
authors.affiliation: Viral Research and Diagnostic Laboratory, Regional Medical research Centre (ICMR), Nalcosquare, Chandrasekharpur, Bhubanewar, 751023, Odisha, India.
authors.forename: Rashmirani
authors.initials: R
authors.lastname: Senapati
authors.affiliation: Department of Gynecologic oncology, Acharya Harihara Regional Cancer Centre, Cuttack, Odisha, India.
authors.forename: Bhagyalaxmi
authors.initials: B
authors.lastname: Nayak
authors.affiliation: Institute of Medical Science and Sum Hospital, Shiksha O Anusandhana University, Bhubanewar, Odisha, India.
authors.forename: Shantanu Kumar
authors.initials: SK
authors.lastname: Kar
authors.affiliation: Viral Research and Diagnostic Laboratory, Regional Medical research Centre (ICMR), Nalcosquare, Chandrasekharpur, Bhubanewar, 751023, Odisha, India. bhagirathidwibedi@yahoo.com.
authors.forename: Bhagirathi
authors.initials: B
authors.lastname: Dwibedi
mesh_terms: D000328:Adult; D005260:Female; D005838:Genotype; D052162:Human papillomavirus 16 / Q000235:genetics / Q000302:isolation & purification; D006801:Humans; D007194:India / Q000453:epidemiology; D008875:Middle Aged; D027383:Papillomaviridae / Q000235:genetics* / Q000302:isolation & purification; D030361:Papillomavirus Infections / Q000453:epidemiology* / Q000821:virology*; D053918:Papillomavirus Vaccines; D012307:Risk Factors; D002583:Uterine Cervical Neoplasms / Q000821:virology*; D014626:Vaginal Smears
chemical_list: D053918:Papillomavirus Vaccines
keywords: Cervical cancer; Eastern India; HPV genotype distribution; Impact of HPV vaccine; Odisha