title: Antimicrobial peptide coatings for hydroxyapatite: electrostatic and covalent attachment of antimicrobial peptides to surfaces.
abstract: The interface between implanted devices and their host tissue is complex and is often optimized for maximal integration and cell adhesion. However, this also gives a surface suitable for bacterial colonization. We have developed a novel method of modifying the surface at the material-tissue interface with an antimicrobial peptide (AMP) coating to allow cell attachment while inhibiting bacterial colonization. The technology reported here is a dual AMP coating. The dual coating consists of AMPs covalently bonded to the hydroxyapatite surface, followed by deposition of electrostatically bound AMPs. The dual approach gives an efficacious coating which is stable for over 12 months and can prevent colonization of the surface by both Gram-positive and Gram-negative bacteria.
authors.affiliation: Institute of Inflammation and Ageing, University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Leigh
authors.initials: L
authors.lastname: Townsend
authors.affiliation: School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Richard L
authors.initials: RL
authors.lastname: Williams
authors.affiliation: Institute of Inflammation and Ageing, University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Olachi
authors.initials: O
authors.lastname: Anuforom
authors.affiliation: School of Chemistry, University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Matthew R
authors.initials: MR
authors.lastname: Berwick
authors.affiliation: University Hospitals Birmingham NHS Foundation Trust, Birmingham B15 2TH, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Fenella
authors.initials: F
authors.lastname: Halstead
authors.affiliation: School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Erik
authors.initials: E
authors.lastname: Hughes
authors.affiliation: School of Metallurgy and Materials, University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Artemis
authors.initials: A
authors.lastname: Stamboulis
authors.affiliation: University Hospitals Birmingham NHS Foundation Trust, Birmingham B15 2TH, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Beryl
authors.initials: B
authors.lastname: Oppenheim
authors.affiliation: School of Materials, University of Manchester, Manchester M1 7HS, UK.
authors.forename: Julie
authors.initials: J
authors.lastname: Gough
authors.affiliation: School of Chemical Engineering, University of Birmingham, Birmingham B15 2TT, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Liam
authors.initials: L
authors.lastname: Grover
authors.affiliation: Royal Centre for Defence Medicine, Birmingham B15 2TH, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Robert A H
authors.initials: RA
authors.lastname: Scott
authors.affiliation: Institute of Microbiology and Infection, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Mark
authors.initials: M
authors.lastname: Webber
authors.affiliation: School of Chemistry, University of Birmingham, Birmingham B15 2TT, UK.
authors.forename: Anna F A
authors.initials: AF
authors.lastname: Peacock
authors.affiliation: Institute of Inflammation and Ageing, University of Birmingham, Birmingham B15 2TT, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Antonio
authors.initials: A
authors.lastname: Belli
authors.affiliation: Institute of Inflammation and Ageing, University of Birmingham, Birmingham B15 2TT, UK.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Ann
authors.initials: A
authors.lastname: Logan
authors.affiliation: Institute of Inflammation and Ageing, University of Birmingham, Birmingham B15 2TT, UK f.decogan@bham.ac.uk.|NIHR Surgical Reconstruction and Microbiology Research Centre, Queen Elizabeth Hospital, Birmingham B15 2TH, UK.
authors.forename: Felicity
authors.identifier: 0000-0001-5083-7130
authors.initials: F
authors.lastname: de Cogan
mesh_terms: D000818:Animals; D023181:Antimicrobial Cationic Peptides / Q000737:chemistry*; D002460:Cell Line; D020099:Coated Materials, Biocompatible / Q000737:chemistry*; D017886:Durapatite / Q000737:chemistry*; D006090:Gram-Negative Bacteria / Q000254:growth & development; D006094:Gram-Positive Bacteria / Q000254:growth & development; D008422:Materials Testing; D051379:Mice; D010006:Osteoblasts / Q000166:cytology / Q000378:metabolism*; D055672:Static Electricity
chemical_list: D023181:Antimicrobial Cationic Peptides; D020099:Coated Materials, Biocompatible; D017886:Durapatite
keywords: antimicrobial; device–tissue interface; surface engineering