title: The influence of selenium status on body composition, oxidative DNA damage and total antioxidant capacity in newly diagnosed type 2 diabetes mellitus: A case-control study.
abstract: Selenium is involved in the complex system of defense against oxidative stress in diabetes through its biological function of selenoproteins and the antioxidant enzyme. A case-control study was carried out to determine the association of plasma selenium with oxidative stress and body composition status presented in Type 2 Diabetes Mellitus (T2DM) patient and healthy control. This study involved 82 newly diagnosed T2DM patients and 82 healthy controls. Plasma selenium status was determined with Graphite Furnace Atomic Absorption Spectrometry. Body Mass Index, total body fat and visceral fat was assessed for body composition using Body Composition Analyzer (TANITA). Oxidative DNA damage and total antioxidant capacity were determined for oxidative stress biomarker status. In age, gender and BMI adjustment, no significant difference of plasma selenium level between T2DM and healthy controls was observed. There was as a significant difference of Oxidative DNA damage and total antioxidant capacity between T2DM patients and healthy controls with tail DNA% 20.62 [95% CI: 19.71,21.49] (T2DM), 17.67 [95% CI: 16.87,18.56] (control); log tail moment 0.41[95% CI: 0.30,0.52] (T2DM), 0.41[95% CI: 0.30,0.52] (control); total antioxidant capacity 0.56 [95% CI: 0.54,0.58] (T2DM), 0.60 [95% CI: 0.57,0.62] (control). Waist circumference, BMI, visceral fat, body fat and oxidative DNA damage in the T2DM group were significantly lower in the first plasma selenium tertile (38.65-80.90μg/L) compared to the second (80.91-98.20μg/L) and the third selenium tertiles (98.21-158.20μg/L). A similar trend, but not statistically significant, was observed in the control group.
authors.affiliation: Centre of Nutrition Epidemiology Research, Institute For Public Health, Ministry of Health, Jalan Bangsar, 50590 Kuala Lumpur, Malaysia; Nutrition and Dietetics Programme, School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia. Electronic address: fatimah.oth@moh.gov.my.
authors.forename: Fatimah Binti
authors.initials: FB
authors.lastname: Othman
authors.affiliation: Nutrition and Dietetics Programme, School of Health Sciences, Universiti Sains Malaysia, Kelantan, Malaysia. Electronic address: hamidjan@usm.my.
authors.forename: Hamid Jan Bin Jan
authors.initials: HJBJ
authors.lastname: Mohamed
authors.affiliation: Department of Chemical Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Kelantan, Malaysia. Electronic address: sirajuden@usm.my.
authors.forename: K N S
authors.initials: KNS
authors.lastname: Sirajudeen
authors.affiliation: Cardiovascular, Diabetes and Nutrition Research Centre, Institute for Medical Research, Kuala Lumpur, Malaysia. Electronic address: fairul@imr.gov.my.
authors.forename: Mohd Fairulnizal B Md
authors.initials: MFBM
authors.lastname: Noh
authors.affiliation: Biomedical Science Program, School of Diagnostic and Applied Health Sciences, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia. Electronic address: nfadilah@ukm.edu.my.
authors.forename: Nor Fadilah
authors.initials: NF
authors.lastname: Rajab
mesh_terms: D000328:Adult; D000975:Antioxidants / Q000378:metabolism*; D001786:Blood Glucose / Q000378:metabolism; D001823:Body Composition / Q000235:genetics / Q000502:physiology; D015992:Body Mass Index; D016022:Case-Control Studies; D004249:DNA Damage / Q000235:genetics; D003924:Diabetes Mellitus, Type 2 / Q000097:blood* / Q000378:metabolism*; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D010084:Oxidation-Reduction; D018384:Oxidative Stress / Q000502:physiology; D012643:Selenium / Q000378:metabolism*; D055105:Waist Circumference
chemical_list: D000975:Antioxidants; D001786:Blood Glucose; D012643:Selenium
keywords: Body composition; Graphite Furnace Atomic Absorption Spectrometry; Oxidative DNA damage; Selenium; Total antioxidant capacity; Type 2 diabetes mellitus