title: Practical Guidelines for Studies on Sandfly-Borne Phleboviruses: Part I: Important Points to Consider Ante Field Work.
abstract: The purpose of this review is to provide practical information to help researchers intending to perform "from field to laboratory" studies on phleboviruses transmitted by sandflies. This guideline addresses the different steps to be considered starting from the field collection of sandflies to the laboratory techniques aiming at the detection, isolation, and characterization of sandfly-borne phleboviruses. In this guideline article, we address the impact of various types of data for an optimal organization of the field work intending to collect wildlife sandflies for subsequent virology studies. Analysis of different data sets should result in the geographic positioning of the trapping stations. The overall planning, the equipment and tools needed, the manpower to be deployed, and the logistics to be anticipated and set up should be organized according to the objectives of the field study for optimal efficiency.
authors.affiliation: 1 UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille Univ. - IRD 190 - Inserm 1207 - EHESP), Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille , Marseille, France .
authors.forename: Nazli
authors.initials: N
authors.lastname: Ayhan
authors.affiliation: 1 UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille Univ. - IRD 190 - Inserm 1207 - EHESP), Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille , Marseille, France .
authors.forename: Amal
authors.initials: A
authors.lastname: Baklouti
authors.affiliation: 2 Centre IRD, UMR MIVEGEC (IRD 224 - CNRS 5290 - Université Montpellier) , Montpellier, France .
authors.forename: Jorian
authors.initials: J
authors.lastname: Prudhomme
authors.affiliation: 3 GmbH , 9931 Außervillgraten, Austria .
authors.forename: Gernot
authors.initials: G
authors.lastname: Walder
authors.affiliation: 4 Centre for Vectors and Infectious Diseases Research, National Institute of Health Ricardo Jorge , Águas de Moura, Portugal .
authors.forename: Fatima
authors.initials: F
authors.lastname: Amaro
authors.affiliation: 5 Ecology Section, ESRL Laboratories, Department of Biology, Faculty of Science, Hacettepe University , Ankara, Turkey .
authors.forename: Bulent
authors.initials: B
authors.lastname: Alten
authors.affiliation: 6 Animal Health Laboratory , UMR BIPAR, ANSES Maisons-Alfort, Paris, France .
authors.forename: Sara
authors.initials: S
authors.lastname: Moutailler
authors.affiliation: 7 Virology Unit, Department of Medical Microbiology, Faculty of Medicine, Hacettepe University , Ankara, Turkey .
authors.forename: Koray
authors.initials: K
authors.lastname: Ergunay
authors.affiliation: 1 UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille Univ. - IRD 190 - Inserm 1207 - EHESP), Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille , Marseille, France .
authors.forename: Remi N
authors.initials: RN
authors.lastname: Charrel
authors.affiliation: 8 Division of Virology, Departments Hygiene, Microbiology and Social Medicine, Innsbruck Medical University , Innsbruck, Austria .
authors.forename: Hartwig
authors.initials: H
authors.lastname: Huemer
mesh_terms: D063147:Animal Distribution; D000818:Animals; D007303:Insect Vectors / Q000821:virology*; D016856:Phlebovirus / Q000235:genetics / Q000502:physiology*; D010802:Phylogeny; D011576:Psychodidae / Q000821:virology*; D012107:Research Design
keywords: Bunyaviridae; Toscana virus; arbovirus(es); field studies; sand fly (flies); vector-borne