title: Kallikrein-related peptidase expression in odontogenic cysts and tumors: An immunohistochemical comparative study.
abstract: OBJECTIVE
  The aim of the present study was to profile the expression of human kallikrein (KLK)-related peptidases (KLK) in odontogenic lesions.
  
  
  METHODS
  Paraffin-embedded, formalin-fixed, non-odontogenic (control) and odontogenic lesions were stained for KLK using a standard immunohistochemical technique. The intensity and proportion of epithelial cells stained was scored. Reverse transcription-polymerase chain reaction was utilized to evaluate KLK 1-15 mRNA expression in ameloblastomas.
  
  
  RESULTS
  KLK 3, 4, 9, 11, and 14 were present in all lesions. KLK 3 staining was increased in ameloblastomas and keratocystic odontogenic tumors. KLK 5 was present only in Keratocystic odontogenic tumor. KLK 6 was significantly higher in ameloblastomas than in other lesions. For KLK 7, keratocystic odontogenic tumors and nasopalatine duct cysts were significantly different. KLK 6, 8, 10, 11, and 13 were significantly higher in ameloblastomas than in other lesions. KLK 9 was increased in keratocystic odontogenic tumors and dentigerous cysts. The expression of KLK 1, 4, 7, 8, 10, and 12 mRNA was found in ameloblastomas.
  
  
  CONCLUSIONS
  The results suggested that KLK 6, 8, 10, and 13 could be involved in the progression of ameloblastomas. KLK 10 could have a greater role in odontogenic lesions, rather than non-odontogenic lesions. Future studies aim to define the specific roles of KLK cascades in odontogenic lesions.
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Western Ontario, London, Ontario, Canada.
authors.forename: Mark Roger
authors.initials: MR
authors.lastname: Darling
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Western Ontario, London, Ontario, Canada.
authors.forename: Rebecca
authors.initials: R
authors.lastname: Woodford
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Western Ontario, London, Ontario, Canada.
authors.forename: Karl Kevin
authors.initials: KK
authors.lastname: Cuddy
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Western Ontario, London, Ontario, Canada.
authors.forename: Linda
authors.initials: L
authors.lastname: Jackson-Boeters
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Western Ontario, London, Ontario, Canada.
authors.forename: Alyssa
authors.initials: A
authors.lastname: Hayter
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Western Ontario, London, Ontario, Canada.
authors.forename: Jeyanth
authors.initials: J
authors.lastname: Inkaran
authors.affiliation: Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, Ontario, Canada.|Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, Ontario, Canada.
authors.forename: Eleftherios P
authors.initials: EP
authors.lastname: Diamandis
authors.affiliation: Department of Pathology and Laboratory Medicine, University of Western Ontario, London, Ontario, Canada.
authors.forename: Zia
authors.initials: Z
authors.lastname: Khan
mesh_terms: D000293:Adolescent; D000328:Adult; D000368:Aged; D005260:Female; D006801:Humans; D007150:Immunohistochemistry; D007610:Kallikreins / Q000032:analysis / Q000096:biosynthesis*; D008297:Male; D008875:Middle Aged; D009807:Odontogenic Cysts / Q000737:chemistry / Q000378:metabolism*; D009808:Odontogenic Tumors / Q000737:chemistry / Q000378:metabolism*
chemical_list: D007610:Kallikreins
keywords: immunohistochemistry, kallikrein; odontogenic cyst; odontogenic tumor; polymerase chain reaction