title: Effects of long non-coding RNA SPRY4-IT1 on osteosarcoma cell biological behavior.
abstract: Recent findings indicate that long noncoding RNAs (lncRNAs) were dysregulated in many kinds of tumors including osteosarcoma (OS). SPRY4-IT1 has been recently revealed as oncogenic regulator in various cancers, while its clinical value and potential function in OS are still unknown. To investigate the role of SPRY4-IT1 in OS, we evaluated the expression SPRY4-IT1 in OS tissues and cell lines, and investigated the effect of SPRY4-IT1 siRNA on cell proliferation, migration and invasion of OSin vitro. Our result showed that SPRY4-IT1 was upregulated in OS tissues. Further experiments revealed that SPRY4-IT1 knockdown significantly inhibited OS cells proliferation by causing G1 arrest and promoting apoptosis. Furthermore, inhibitory effects of SPRY4-IT1 on cell migration and invasion were partly associated with EMT process. In conclusion, these data suggest that SPRY4-IT1 could be an oncogene for OS, and may be served as a candidate target for new therapies in human OS.
authors.affiliation: Department of Orthopedics, Baoshan District Shanghai Integrated Traditional Chinese and Western Medicine Hospital Shanghai, China.
authors.forename: Jin
authors.initials: J
authors.lastname: Xu
authors.affiliation: Department of Orthopedics, Baoshan District Shanghai Integrated Traditional Chinese and Western Medicine Hospital Shanghai, China.
authors.forename: Ren
authors.initials: R
authors.lastname: Ding
authors.affiliation: Department of Orthopedics, The First Affiliated Hospital of Soochow University Suzhou, China.
authors.forename: Yaozeng
authors.initials: Y
authors.lastname: Xu
keywords: EMT; SPRY4-IT1; apoptosis; long noncoding RNA; migration; osteosarcoma; proliferation