title: RP-HPLC-DAD-ESI-QTOF-MS based metabolic profiling of the potential Olea europaea by-product "wood" and its comparison with leaf counterpart.
abstract: BACKGROUND
  Olea europaea L. organs such as leaves, stems and roots have been associated with numerous in vivo and in vitro biological activities and used for traditional medicinal purposes. However, tree wood is an untapped resource with little information about their chemical composition.
  
  
  OBJECTIVE
  That is why, the objective of this study is to increase the knowledge about phytochemicals from 'Chemlali' olive wood by means of mass spectrometry-based analyses. Its comparison with by-products derived from leaves was also studied.
  
  
  METHODS
  Hydromethanol extracts from wood and leaves with stems of 'Chemlali' olive cultivar were analysed using reversed-phase (RP) high-performance liquid chromatography (HPLC) coupled to two detection systems: diode-array detection (DAD) and quadrupole time-of-flight (QTOF) mass spectrometry (MS) in negative ion mode. Tandem MS experiments were performed to establish the chemical structure of olive phytochemicals.
  
  
  RESULTS
  A total of 85 compounds were characterised in the studied olive parts and classified as: sugars (3), organic acids (5), one phenolic aldehyde, simple phenolic acids (6), simple phenylethanoids (5), flavonoids (14), coumarins (3), caffeoyl phenylethanoid derivatives (6), iridoids (5), secoiridoids (32), and lignans (5). To our knowledge, the major part of these metabolites was not previously reported in olive tree wood, and 10 olive chemical constituents were identified for the first time in the Oleaceae family.
  
  
  CONCLUSIONS
  The results presented here demonstrated the usefulness of the methodology proposed, based on RP-HPLC-DAD-ESI-QTOF-MS and MS/MS, to develop an exhaustive metabolic profiling and to recover new biologically active compounds in olive wood with pharmacologic and cosmetic potential. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016, Granada, Spain.|Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP "1173", 3038, Tunisia.
authors.forename: Sonda
authors.initials: S
authors.lastname: Ammar
authors.affiliation: Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016, Granada, Spain.|Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.|Department of Analytical Chemistry, University of Cordoba, Annex C-3 Building, Campus of Rabanales, 14071, Córdoba, Spain.
authors.forename: Maria Del Mar
authors.initials: MDM
authors.lastname: Contreras
authors.affiliation: Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP "1173", 3038, Tunisia.
authors.forename: Boutheina
authors.initials: B
authors.lastname: Gargouri
authors.affiliation: Research and Development Functional Food Centre (CIDAF), Bioregión Building, Health Science Technological Park, Avenida del Conocimiento s/n, 18016, Granada, Spain.|Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avda. Fuentenueva s/n, 18071, Granada, Spain.
authors.forename: Antonio
authors.initials: A
authors.lastname: Segura-Carretero
authors.affiliation: Laboratoire d'Electrochimie et Environnement, Ecole Nationale d'Ingénieurs de Sfax, Université de Sfax, BP "1173", 3038, Tunisia.
authors.forename: Mohamed
authors.initials: M
authors.lastname: Bouaziz
mesh_terms: D002851:Chromatography, High Pressure Liquid / Q000379:methods; D056148:Chromatography, Reverse-Phase / Q000379:methods; D003374:Coumarins / Q000032:analysis / Q000737:chemistry; D005419:Flavonoids / Q000032:analysis / Q000737:chemistry; D039823:Iridoids / Q000032:analysis / Q000737:chemistry; D017705:Lignans / Q000032:analysis / Q000737:chemistry; D055432:Metabolomics / Q000379:methods*; D031658:Olea / Q000737:chemistry*; D010636:Phenols / Q000032:analysis / Q000737:chemistry; D064209:Phytochemicals / Q000032:analysis* / Q000737:chemistry; D018515:Plant Leaves / Q000737:chemistry*; D021241:Spectrometry, Mass, Electrospray Ionization / Q000379:methods; D053719:Tandem Mass Spectrometry / Q000379:methods; D014934:Wood / Q000737:chemistry*
chemical_list: D003374:Coumarins; D005419:Flavonoids; D039823:Iridoids; D017705:Lignans; D010636:Phenols; D064209:Phytochemicals
keywords: olive leaves; olive wood; phytochemicals; secoiridoids; tandem mass spectrometry