title: In vitroDegradation of Antimicrobials during Use of Broth Microdilution Method Can Increase the Measured Minimal Inhibitory and Minimal Bactericidal Concentrations.
abstract: The antibacterial activity of some antimicrobials may be under-estimated duringin vitromicrobiological susceptibility tests, due to their instability under such conditions. Thein vitrodegradation of seven widely used antimicrobials (amoxicillin, cephalexin monohydrate, cefotaxime sodium salt, ciprofloxacin, erythromycin hydrate, clarithromycin, and doxycycline hyclate) and its effect on MIC and MBC determinations was studied using the broth microdilution method, considered as the gold standard for MIC determinations.In vitroconcentrations of antimicrobials, over a 24 h incubation period in the medium tested without bacteria, decreased from 33% for ciprofloxacin to 69% for clarithromycin. For cephalexin, cefotaxime, clarithromycin, and doxycycline which were the most degraded drugs, MIC and MBC values for one strain ofE. coliand one strain ofS. aureuswere compared using the standard method or afterad-hocdrug complementation aiming at maintaining constant drug concentration. Abiotic degradation during the standard method was associated with a significant increase of the MIC (2 antibiotics) and MBC (3 antibiotics). However, the observed discrepancy (less than one twofold dilution), even for the most degraded drug for which the concentration at 24 h was reduced by two thirds, suggests that this would only be clinically significant in special cases such as slow-growing bacteria.
authors.affiliation: TOXALIM, Université de Toulouse, Institut National de la Recherche Agronomique, ENVT, INP-Purpan, UPS Toulouse, France.
authors.forename: Elodie A
authors.initials: EA
authors.lastname: Lallemand
authors.affiliation: TOXALIM, Université de Toulouse, Institut National de la Recherche Agronomique, ENVT, INP-Purpan, UPS Toulouse, France.
authors.forename: Marlène Z
authors.initials: MZ
authors.lastname: Lacroix
authors.affiliation: TOXALIM, Université de Toulouse, Institut National de la Recherche Agronomique, ENVT, INP-Purpan, UPS Toulouse, France.
authors.forename: Pierre-Louis
authors.initials: PL
authors.lastname: Toutain
authors.affiliation: Université de Toulouse, ENVT Toulouse, France.
authors.forename: Séverine
authors.initials: S
authors.lastname: Boullier
authors.affiliation: TOXALIM, Université de Toulouse, Institut National de la Recherche Agronomique, ENVT, INP-Purpan, UPS Toulouse, France.
authors.forename: Aude A
authors.initials: AA
authors.lastname: Ferran
authors.affiliation: TOXALIM, Université de Toulouse, Institut National de la Recherche Agronomique, ENVT, INP-Purpan, UPS Toulouse, France.
authors.forename: Alain
authors.initials: A
authors.lastname: Bousquet-Melou
keywords: MBC; MIC; antimicrobial; degradation; stability