title: Development of a novel model of hypertriglyceridemic acute pancreatitis in mice.
abstract: The morbidity rate of hypertriglyceridemic acute pancreatitis (HTG-AP) increased rapidly over the last decade. However an appropriate animal model was lacking to recapitulate this complicated human disease. We established a novel mice model of HTG-AP by poloxamer 407 (P-407) combined with caerulein (Cae). In our study, serum triglyceride levels of P-407 induced mice were elevated in a dose-dependent manner, and the pancreatic and pulmonary injuries were much severer in HTG mice than normal mice when injected with conventional dose Cae (50 ug/kg), what's more, the severity of AP was positively correlative with duration and extent of HTG. In addition, we found that a low dose Cae (5 ug/kg) could induce pancreatic injury in HTG mice while there was no obvious pathological injury in normal mice. Finally, we observed that HTG leaded to the increased infiltrations of macrophages and neutrophils in mice pancreatic tissues. In conclusion, we have developed a novel animal model of HTG-AP that can mimic physiological, histological, clinical features of human HTG-AP and it could promote the development of therapeutic strategies and advance the mechanism research on HTG-AP.
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Yiyuan
authors.initials: Y
authors.lastname: Pan
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Li
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Lin
authors.initials: L
authors.lastname: Gao
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Zhihui
authors.initials: Z
authors.lastname: Tong
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Bo
authors.initials: B
authors.lastname: Ye
authors.affiliation: Center for Immunology and Infectious Diseases, Biosciences Division, SRI International, Harrisonburg, VA 22802, USA.
authors.forename: Shufeng
authors.initials: S
authors.lastname: Liu
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Baiqiang
authors.initials: B
authors.lastname: Li
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Yizhe
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Yang
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Lei
authors.initials: L
authors.lastname: Meng
authors.affiliation: Institute of Cardiovascular Science, Key Laboratory of Molecular Cardiovascular Science Ministry of Education, Institute of Cardiovascular Science, Peking University, Beijing, 100191, China.
authors.forename: Yuhui
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Institute of Cardiovascular Science, Key Laboratory of Molecular Cardiovascular Science Ministry of Education, Institute of Cardiovascular Science, Peking University, Beijing, 100191, China.
authors.forename: George
authors.initials: G
authors.lastname: Liu
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.|Department of Gastroenterology, The Second Clinical Medical College, Yangzhou University, Yangzhou, China.
authors.forename: Guotao
authors.initials: G
authors.lastname: Lu
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Weiqin
authors.initials: W
authors.lastname: Li
authors.affiliation: Surgical Intensive Care Unit (SICU), Department of General Surgery, Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing 210002, Jiangsu Province, China.
authors.forename: Jieshou
authors.initials: J
authors.lastname: Li
mesh_terms: D000208:Acute Disease; D000818:Animals; D004195:Disease Models, Animal; D004198:Disease Susceptibility; D015228:Hypertriglyceridemia / Q000139:chemically induced / Q000150:complications*; D008079:Lipoproteins, VLDL / Q000009:adverse effects; D008264:Macrophages / Q000473:pathology; D051379:Mice; D020556:Neutrophil Infiltration; D010195:Pancreatitis / Q000209:etiology* / Q000473:pathology*; D012720:Severity of Illness Index
chemical_list: D008079:Lipoproteins, VLDL