title: Bacterial community structure in simultaneous nitrification, denitrification and organic matter removal process treating saline mustard tuber wastewater as revealed by 16S rRNA sequencing.
abstract: A simultaneous nitrification, denitrification and organic matter removal (SNDOR) process in sequencing batch biofilm reactor (SBBR) was established to treat saline mustard tuber wastewater (MTWW) in this study. An average COD removal efficiency of 86.48% and total nitrogen removal efficiency of 86.48% were achieved at 30gNaClL-1during 100days' operation. The underlying mechanisms were investigated by PacBio SMRT DNA sequencing (V1-V9) to analyze the microbial community structures and its variation from low salinity at 10gNaClL-1to high salinity at 30gNaClL-1. Results showed elevated salinity did not affect biological performance but reduced microbial diversity in SBBR, and halophilic bacteria gradually predominated by succession. Despite of high C/N, autotrophic ammonia-oxidizing bacteria (AOB) Nitrosomonas and ammonia-oxidizing archaea (AOA) Candidatus Nitrososphaera both contributed to ammonium oxidation. As salinity increasing, nitrite-oxidizing bacteria (NOB) were significantly inhibited, partial nitrification and denitrification (PND) process gradually contributed to nitrogen removal.
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China.
authors.forename: Jiale
authors.initials: J
authors.lastname: Wang
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China.
authors.forename: Benzhou
authors.initials: B
authors.lastname: Gong
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China.
authors.forename: Wei
authors.initials: W
authors.lastname: Huang
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China.
authors.forename: Yingmu
authors.initials: Y
authors.lastname: Wang
authors.affiliation: Faculty of Urban Construction and Environmental Engineering, Chongqing University, Chongqing 400045, PR China; Key Laboratory of the Three Gorges Reservoir's Eco-Environments, Ministry of Education, Chongqing University, Chongqing 400045, PR China. Electronic address: zhoujiantg@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Zhou
mesh_terms: D000641:Ammonia / Q000737:chemistry / Q000378:metabolism; D001105:Archaea / Q000235:genetics / Q000378:metabolism; D052818:Autotrophic Processes; D001419:Bacteria / Q000235:genetics / Q000378:metabolism; D019149:Bioreactors / Q000382:microbiology; D058440:Denitrification; D005525:Food-Processing Industry; D059013:Microbial Consortia / Q000235:genetics*; D009149:Mustard Plant / Q000737:chemistry; D058465:Nitrification; D009584:Nitrogen / Q000378:metabolism; D010084:Oxidation-Reduction; D012336:RNA, Ribosomal, 16S; D054712:Salinity; D014865:Waste Disposal, Fluid / Q000295:instrumentation / Q000379:methods*; D062065:Wastewater / Q000737:chemistry
chemical_list: D000641:Ammonia; D009584:Nitrogen; D012336:RNA, Ribosomal, 16S; D062065:Wastewater
keywords: Archaea; Full-length 16S rRNA sequencing; Microbial community; Partial nitrification and denitrification; Saline mustard tuber wastewater