title: Regulation of FADS2 transcription by SREBP-1 and PPAR-α influences LC-PUFA biosynthesis in fish.
abstract: The present study was conducted to explore the mechanisms leading to differences among fishes in the ability to biosynthesize long-chain polyunsaturated fatty acids (LC-PUFAs). Replacement of fish oil with vegetable oil caused varied degrees of increase in 18-carbon fatty acid content and decrease in n-3 LC-PUFA content in the muscle and liver of rainbow trout (Oncorhynchus mykiss), Japanese seabass (Lateolabrax japonicus) and large yellow croaker (Larimichthys crocea), suggesting that these fishes have differing abilities to biosynthesize LC-PUFAs. Fish oil replacement also led to significantly up-regulated expression of FADS2 and SREBP-1 but different responses of the two PPAR-α homologues in the livers of these three fishes. An in vitro experiment indicated that the basic transcription activity of the FADS2 promoter was significantly higher in rainbow trout than in Japanese seabass or large yellow croaker, which was consistent with their LC-PUFA biosynthetic abilities. In addition, SREBP-1 and PPAR-α up-regulated FADS2 promoter activity. These regulatory effects varied considerably between SREBP-1 and PPAR-α, as well as among the three fishes. Taken together, the differences in regulatory activities of the two transcription factors targeting FADS2 may be responsible for the different LC-PUFA biosynthetic abilities in these three fishes that have adapted to different ambient salinity.
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Xiaojing
authors.initials: X
authors.lastname: Dong
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Peng
authors.initials: P
authors.lastname: Tan
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Zuonan
authors.initials: Z
authors.lastname: Cai
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Hanlin
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Jingqi
authors.initials: J
authors.lastname: Li
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Wei
authors.initials: W
authors.lastname: Ren
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Houguo
authors.initials: H
authors.lastname: Xu
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Rantao
authors.initials: R
authors.lastname: Zuo
authors.affiliation: Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Jianfeng
authors.initials: J
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.|Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Kangsen
authors.initials: K
authors.lastname: Mai
authors.affiliation: Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, People's Republic of China.|Laboratory for Marine Fisheries and Aquaculture, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266003, People's Republic of China.
authors.forename: Qinghui
authors.initials: Q
authors.lastname: Ai
mesh_terms: D000818:Animals; D001483:Base Sequence; D004041:Dietary Fats / Q000494:pharmacology; D044943:Fatty Acid Desaturases / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D005227:Fatty Acids / Q000378:metabolism; D015525:Fatty Acids, Omega-3 / Q000096:biosynthesis*; D029941:Fish Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000187:drug effects / Q000378:metabolism; D009132:Muscles / Q000187:drug effects / Q000378:metabolism; D017686:Oncorhynchus mykiss / Q000254:growth & development / Q000378:metabolism*; D047493:PPAR alpha / Q000145:classification / Q000235:genetics / Q000378:metabolism*; D010473:Perciformes / Q000254:growth & development / Q000378:metabolism*; D010802:Phylogeny; D010938:Plant Oils / Q000494:pharmacology; D011401:Promoter Regions, Genetic; D016415:Sequence Alignment; D051780:Sterol Regulatory Element Binding Protein 1 / Q000235:genetics / Q000378:metabolism*; D014158:Transcription, Genetic / Q000187:drug effects
chemical_list: D004041:Dietary Fats; D005227:Fatty Acids; D015525:Fatty Acids, Omega-3; D029941:Fish Proteins; D047493:PPAR alpha; D010938:Plant Oils; D051780:Sterol Regulatory Element Binding Protein 1; D044943:Fatty Acid Desaturases