title: Maternal obesity in the rat impairs male offspring aging of the testicular antioxidant defence system.
abstract: A high-fat diet during intrauterine development predisposes offspring (F1) to phenotypic alterations, such as lipid synthesis imbalance and increased oxidative stress, causing changes in male fertility. The objective of this study was to evaluate the effects of maternal obesity during pregnancy and lactation on antioxidant enzymes in the F1testes. Female Wistar rats (F0) were fed either a control (C, 5% fat) or an obesogenic (MO, maternal obesity, 25% fat) diet from weaning and throughout subsequent pregnancy and lactation. F1offspring were weaned to the control diet. Testes were retrieved at 110, 450 and 650 postnatal days (PND) for real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) and immunohistochemical (IHC) antioxidant enzyme analyses. Catalase was similar between groups by RT-qPCR, whereas by IHC it was higher in the MO group at all ages than in the C group. Superoxide dismutase 1 (SOD1) had lower expression at PND 110 in MO than in C by both techniques; at PND 450 and 650 by immunoanalysis SOD1 was higher in MO than in C. Glutathione peroxidase 1 (GPX1), GPX2 and GPX4 by RT-qPCR were similar between groups and ages; by IHC GPX1/2 was higher in MO than in C, whereas GPX4 showed the opposite result at PND 110 and 450. In conclusion, antioxidant enzymes in the rat testes are modified with age. Maternal obesity negatively affects the F1testicular antioxidant defence system, which, in turn, can explain the decrease in reproductive capacity.
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Biología de la Reproducción, Vasco de Quiroga 15, Belisario Domínguez, Tlalpan, 14080, México, D.F. México.
authors.forename: Claudia J
authors.initials: CJ
authors.lastname: Bautista
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Biología de la Reproducción, Vasco de Quiroga 15, Belisario Domínguez, Tlalpan, 14080, México, D.F. México.
authors.forename: Guadalupe L
authors.initials: GL
authors.lastname: Rodríguez-González
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Biología de la Reproducción, Vasco de Quiroga 15, Belisario Domínguez, Tlalpan, 14080, México, D.F. México.
authors.forename: Angélica
authors.initials: A
authors.lastname: Morales
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Biología de la Reproducción, Vasco de Quiroga 15, Belisario Domínguez, Tlalpan, 14080, México, D.F. México.
authors.forename: Consuelo
authors.initials: C
authors.lastname: Lomas-Soria
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Biología de la Reproducción, Vasco de Quiroga 15, Belisario Domínguez, Tlalpan, 14080, México, D.F. México.
authors.forename: Fabiola
authors.initials: F
authors.lastname: Cruz-Pérez
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Biología de la Reproducción, Vasco de Quiroga 15, Belisario Domínguez, Tlalpan, 14080, México, D.F. México.
authors.forename: Luis A
authors.initials: LA
authors.lastname: Reyes-Castro
authors.affiliation: Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Departamento de Biología de la Reproducción, Vasco de Quiroga 15, Belisario Domínguez, Tlalpan, 14080, México, D.F. México.
authors.forename: Elena
authors.initials: E
authors.lastname: Zambrano
mesh_terms: D000375:Aging / Q000378:metabolism; D000818:Animals; D000975:Antioxidants / Q000378:metabolism*; D002374:Catalase / Q000378:metabolism; D059305:Diet, High-Fat; D005260:Female; D005979:Glutathione Peroxidase / Q000378:metabolism; D008297:Male; D039382:Maternal Nutritional Physiological Phenomena / Q000502:physiology*; D009765:Obesity / Q000378:metabolism*; D018384:Oxidative Stress / Q000502:physiology*; D011247:Pregnancy; D011297:Prenatal Exposure Delayed Effects / Q000378:metabolism*; D051381:Rats; D017208:Rats, Wistar; D013482:Superoxide Dismutase / Q000378:metabolism; D013737:Testis / Q000378:metabolism*; D000094765:Glutathione Peroxidase GPX1
chemical_list: D000975:Antioxidants; D002374:Catalase; D005979:Glutathione Peroxidase; D013482:Superoxide Dismutase; D000094765:Glutathione Peroxidase GPX1; C000722069:Gpx1 protein, rat