title: Green Sonoextraction of Protein from Oleaginous Press Rapeseed Cake.
abstract: In this study, extraction of soluble proteins from rapeseed cake using different conventional and innovative extraction processes in order to maximize the extraction yield has been investigated. Firstly, various extraction techniques including ultrasound, microwave, and percolation were tested to increase the protein recovery efficiency. Secondly, response surface methodology (RSM) using a central composite design (CCD) approach was applied to investigate the influence of process variables on ultrasound-assisted extraction (UAE). Statistical analysis revealed that the optimized conditions providing a protein yield of 4.24 g/100 g DM were an ultrasound power of 5.6 W·cm-2and temperature of 45 °C. Quantitatively UAE followed by two stages of conventional extraction gave the best total protein yield of 9.81 g/100 g DM. Qualitatively, the protein efficiency ratio (PER) used as measure of the nutritive value (12S/2S ratio) which indicates protein quality in terms of S-containing essential amino acids, was similar to that of the conventional extraction method. Small amounts of protein aggregate were observed in the HPLC profile of the extract.
authors.affiliation: GREEN Extraction Team, Avignon University, INRA, UMR408, F-84000 Avignon, France. meriem.boukroufa@alumni.univ-avignon.fr.
authors.forename: Meryem
authors.initials: M
authors.lastname: Boukroufa
authors.affiliation: Terres Inovia, 33600 Pessac, France. ag.sicaire@terresinovia.fr.
authors.forename: Anne-Gaëlle
authors.initials: AG
authors.lastname: Sicaire
authors.affiliation: Terres Inovia, 33600 Pessac, France. f.fine@terresinovia.fr.
authors.forename: Frederic
authors.initials: F
authors.lastname: Fine
authors.affiliation: INRA, UR 1268 Biopolymeres Interactions Assemblages, 44316 Nantes CEDEX 3, France. colette.larre@inra.fr.
authors.forename: Colette
authors.initials: C
authors.lastname: Larré
authors.affiliation: INRA, UR 1268 Biopolymeres Interactions Assemblages, 44316 Nantes CEDEX 3, France. aude.le-goff@inra.fr.
authors.forename: Aude Le
authors.initials: AL
authors.lastname: Goff
authors.affiliation: INRA, UR 1268 Biopolymeres Interactions Assemblages, 44316 Nantes CEDEX 3, France. veronique.sole@inra.fr.
authors.forename: Véronique Solé
authors.initials: VS
authors.lastname: Jamault
authors.affiliation: GREEN Extraction Team, Avignon University, INRA, UMR408, F-84000 Avignon, France. njara.rakotomanomana@univ-avignon.fr.
authors.forename: Njara
authors.initials: N
authors.lastname: Rakotomanomana
authors.affiliation: GREEN Extraction Team, Avignon University, INRA, UMR408, F-84000 Avignon, France. farid.chemat@univ-avignon.fr.
authors.forename: Farid
authors.initials: F
authors.lastname: Chemat
mesh_terms: D029741:Brassica rapa / Q000737:chemistry*; D002851:Chromatography, High Pressure Liquid; D005163:Factor Analysis, Statistical; D055772:Green Chemistry Technology; D008872:Microwaves; D010936:Plant Extracts / Q000737:chemistry*; D010940:Plant Proteins / Q000302:isolation & purification*; D052616:Solid Phase Extraction / Q000379:methods*; D013010:Sonication; D013696:Temperature
chemical_list: D010936:Plant Extracts; D010940:Plant Proteins
keywords: green extraction; microwave; protein; rapeseed; ultrasound