title: Progress in bio-manufacture of platelets for transfusion.
abstract: Blood transfusion services face an ever-increasing demand for donor platelets to meet clinical needs. Whilst strategies for increasing platelet storage life and improving the efficiency of donor platelet collection are important, in the longer term, platelets generated by bio-manufacturing processes will be required to meet demands. Production of sufficient numbers of in vitro-derived platelets for transfusion represents a significant bioengineering challenge. In this review, we highlight recent progress in this area of research and outline the main technical and biological obstacles that need to be met before this becomes feasible and economic. A critical consideration is assurance of the functional properties of these cells as compared to their fresh, donor collected, counterparts. We contend that platelet-like particles and in vitro-derived platelets that phenotypically resemble fresh platelets must deliver the same functions as these cells upon transfusion. We also note recent progress with immortalized megakaryocyte progenitor cell lines, molecular strategies for reducing expression of HLA Class I to generate universal donor platelets and the move to early clinical studies with in vitro-derived platelets.
authors.affiliation: a Manufacturing , Commonwealth Scientific Industrial Research Organisation , Clayton , Australia.|b The Australian Regenerative Medicine Institute, Monash University , Clayton , Australia.
authors.forename: Shen Y
authors.initials: SY
authors.lastname: Heazlewood
authors.affiliation: a Manufacturing , Commonwealth Scientific Industrial Research Organisation , Clayton , Australia.|b The Australian Regenerative Medicine Institute, Monash University , Clayton , Australia.
authors.forename: Susan K
authors.initials: SK
authors.lastname: Nilsson
authors.affiliation: a Manufacturing , Commonwealth Scientific Industrial Research Organisation , Clayton , Australia.
authors.forename: Kellie
authors.initials: K
authors.lastname: Cartledge
authors.affiliation: a Manufacturing , Commonwealth Scientific Industrial Research Organisation , Clayton , Australia.
authors.forename: Cheang Ly
authors.initials: CL
authors.lastname: Be
authors.affiliation: a Manufacturing , Commonwealth Scientific Industrial Research Organisation , Clayton , Australia.|b The Australian Regenerative Medicine Institute, Monash University , Clayton , Australia.
authors.forename: Andrew
authors.initials: A
authors.lastname: Vinson
authors.affiliation: a Manufacturing , Commonwealth Scientific Industrial Research Organisation , Clayton , Australia.
authors.forename: Murat
authors.initials: M
authors.lastname: Gel
authors.affiliation: a Manufacturing , Commonwealth Scientific Industrial Research Organisation , Clayton , Australia.|b The Australian Regenerative Medicine Institute, Monash University , Clayton , Australia.
authors.forename: David N
authors.initials: DN
authors.lastname: Haylock
mesh_terms: D017667:Adipocytes / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D001792:Blood Platelets / Q000166:cytology* / Q000276:immunology; D018929:Cell Culture Techniques; D054337:Cell Dedifferentiation / Q000187:drug effects; D002454:Cell Differentiation / Q000187:drug effects; D002461:Cell Line, Transformed; D016207:Cytokines / Q000494:pharmacology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D020868:Gene Silencing; D015395:Histocompatibility Antigens Class I / Q000235:genetics / Q000276:immunology; D006801:Humans; D057026:Induced Pluripotent Stem Cells / Q000166:cytology / Q000187:drug effects / Q000276:immunology; D036341:Intercellular Signaling Peptides and Proteins / Q000494:pharmacology; D008533:Megakaryocytes / Q000166:cytology* / Q000187:drug effects / Q000276:immunology; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods; D017713:Platelet Transfusion / Q000592:standards* / Q000706:statistics & numerical data
chemical_list: D016207:Cytokines; D015395:Histocompatibility Antigens Class I; D036341:Intercellular Signaling Peptides and Proteins
keywords: Bio-manufacture; platelet; recent advances; transfusion