title: Developmental expression of B cell molecules in equine lymphoid tissues.
abstract: Identification and classification of B cell subpopulations has been shown to be challenging and inconsistent among different species. Our study tested aspects of ontogeny, phenotype, tissue distribution, and function of equine CD5hiB cells, which represented a greater proportion of B cells early in development and in the peritoneal cavity. CD5hiand CD5loB cells differentially expressed B cell markers (CD2, CD21, IgM) measured using flow cytometry, but similar mRNA expression of signature genes (DGKA, FGL2, PAX5, IGHM, IL10) measured using quantitative RT-PCR. Sequencing lambda light chain segments revealed that CD5hiB cells generated diverse immunoglobulin repertoires, and more frequently bound to fluorescence-labeled phosphorylcholine. This study shows developmental characteristics and tissue distribution of a newly described subpopulation of B cells in the horse.
authors.affiliation: Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. Electronic address: jmb279@cornell.edu.
authors.forename: J M B
authors.initials: JMB
authors.lastname: Prieto
authors.affiliation: Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. Electronic address: rlt8@cornell.edu.
authors.forename: R L
authors.initials: RL
authors.lastname: Tallmadge
authors.affiliation: Equine Immunology Laboratory, Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA. Electronic address: mbf6@cornell.edu.
authors.forename: M J B
authors.initials: MJB
authors.lastname: Felippe
mesh_terms: D000375:Aging / Q000276:immunology; D000818:Animals; D016175:B-Lymphocyte Subsets / Q000166:cytology; D001402:B-Lymphocytes / Q000166:cytology* / Q000276:immunology; D018956:CD5 Antigens / Q000096:biosynthesis; D005434:Flow Cytometry / Q000662:veterinary; D006736:Horses / Q000196:embryology / Q000254:growth & development* / Q000276:immunology*; D007147:Immunoglobulin Light Chains / Q000235:genetics; D016130:Immunophenotyping / Q000662:veterinary; D008221:Lymphoid Tissue / Q000166:cytology* / Q000196:embryology; D010767:Phosphorylcholine / Q000378:metabolism
chemical_list: D018956:CD5 Antigens; D007147:Immunoglobulin Light Chains; D010767:Phosphorylcholine
keywords: B cell; CD5; Development; Equine; Immunoglobulin repertoire