title: Inhibitory Effects of Probiotic Lactobacillus on the Growth of Human Colonic Carcinoma Cell Line HT-29.
abstract: This study was conducted to investigate the inhibitory effect ofLactobacilluscells and supernatants on the growth of the human colon cancer cell line HT-29. Our study results indicated that the PM153 strain exhibits the best adhesion ability and the highest survival in the gastrointestinal tract simulation experiment. Furthermore, after an 8-h co-culture of PM153 and HT-29 cells, the PM153 strain can induce the secretion of nitric oxide from the HT-29 cells. In addition, after the co-culture of the BCRC17010 strain (10⁸ cfu/mL) and HT-29 cells, the Bax/Bcl-2 ratio in the HT-29 cells was 1.19, which showed a significant difference from the other control and LAB groups (p< 0.05), which therefore led to the inference that the BCRC17010 strain exerts a pro-apoptotic effect on the HT-29 cells. Upon co-culture with HT-29 cells for 4, 8 and 12 h, the BCRC14625 strain (10⁸ cfu/mL) demonstrated a significant increase in lactate dehydrogenase (LDH) activity (p< 0.05), causing harm to the HT-29 cell membrane; further, after an 8-h co-culture with the HT-29 cells, it induced the secretion of nitric oxide (NO) from the HT-29 cells. Some lactic acid bacteria (LAB) strains have ability to inhibit the growth of the colorectal cancer cell line HT-29 Bax/Bcl-2 pathway or NO production. In summary, we demonstrated that the BCRC17010 strain, good abilities of adhesion and increased LDH release, was the best probiotic potential for inhibition of HT-29 growth amongst the seven LAB strains tested in vitro.
authors.affiliation: Department of Food and Nutrition, Providence University, Taichung City 43301, Taiwan. g1010046@pu.edu.tw.
authors.forename: Zhung-Yuan
authors.initials: ZY
authors.lastname: Chen
authors.affiliation: Department of Food and Nutrition, Providence University, Taichung City 43301, Taiwan. ymhsieh@pu.edu.tw.
authors.forename: You-Miin
authors.initials: YM
authors.lastname: Hsieh
authors.affiliation: New Bellus Enterprises Co., Ltd. No. 48, Industrial Rd., Erh Chen Vil., Kuan Tien Dist., Tainan City 72042, Taiwan. john@newbellus.com.tw.
authors.forename: Chun-Chih
authors.initials: CC
authors.lastname: Huang
authors.affiliation: Department of Food Science and Technology, Hungkuang University, Taichung City 43302, Taiwan. tsaicc@sunrise.hk.edu.tw.
authors.forename: Cheng-Chih
authors.initials: CC
authors.lastname: Tsai
mesh_terms: D001422:Bacterial Adhesion / Q000187:drug effects; D002277:Carcinoma / Q000188:drug therapy* / Q000382:microbiology; D018920:Coculture Techniques / Q000379:methods; D003110:Colonic Neoplasms / Q000188:drug therapy* / Q000201:enzymology / Q000473:pathology; D015972:Gene Expression Regulation, Neoplastic / Q000187:drug effects; D019073:HT29 Cells; D006801:Humans; D007778:Lactobacillus / Q000737:chemistry; D009363:Neoplasm Proteins / Q000096:biosynthesis* / Q000235:genetics; D019936:Probiotics / Q000008:administration & dosage*
chemical_list: D009363:Neoplasm Proteins
keywords: Bax/Bcl-2 ratio; HT-29 cells; Lactobacillus; interleukin-8; lactate dehydrogenase