title: Structural characterization and antioxidant activity of polysaccharide from Hohenbuehelia serotina.
abstract: Previous research found that the polysaccharides isolated from Hohenbuehelia serotina possessed various biological activities, such as antioxidant, immunomodulation and radioprotective effects. However, the structural information of H. serotina polysaccharides has not yet been reported. Therefore, based on the investigation of the antioxidative tracking, a novel polysaccharide named as NTHSP-A1 was isolated from H. serotina by ultrasonic-assistance extraction and anion-exchange and gel permeation chromatography approaches. Structural characterization revealed that NTHSP-A1 had an average molecular weight of 8.09×103Da and composed of arabinose, mannose, glucose and galactose in a molar ratio of 4:16:28:11. The polysaccharide was semi-crystalline substance with multi-branching structure. The backbone of NTHSP-A1 was shown to contain →3,6)-α-d-Glcp-(1→, with branches substituted at C-3 of →2)-α-l-Arap-(1→, C-3 of α-d-Manp-(1→, and C-6 of →6)-β-d-Galp-(1→, respectively. This study provides a theoretical basis for the further research on the relationship between biological activity and structure of NTHSP-A1.
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China. Electronic address: lixiaoyu@ysu.edu.cn.
authors.forename: Xiaoyu
authors.initials: X
authors.lastname: Li
authors.affiliation: School of Environmental and Chemical Engineering, Yanshan University, Qinhuangdao 066004, PR China.
authors.forename: Lu
authors.initials: L
authors.lastname: Wang
authors.affiliation: School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
authors.forename: Zhenyu
authors.initials: Z
authors.lastname: Wang
mesh_terms: D000363:Agaricales / Q000737:chemistry*; D000975:Antioxidants / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D002240:Carbohydrate Sequence; D062610:Fungal Polysaccharides / Q000737:chemistry* / Q000302:isolation & purification / Q000494:pharmacology*; D008745:Methylation; D008970:Molecular Weight; D009005:Monosaccharides / Q000032:analysis
chemical_list: D000975:Antioxidants; D062610:Fungal Polysaccharides; D009005:Monosaccharides
keywords: Antioxidant activity; Polysaccharide; Structural characterization