title: The clinical significance and underlying correlation of pStat-3 and integrin αvβ6 expression in gallbladder cancer.
abstract: BACKGROUND
  Both phosphorylated signal transducer and activator of transcription 3(pStat-3) and integrin αvβ6 can play vital role in the development and progression of cancer. However, little is known about their expression correlation and clinical significance in gallbladder cancer(GBC).
  
  
  OBJECTIVE
  The aim of our present study was to investigate the expression of pStat-3 and integrin αvβ6, two proteins' correlation and their clinical significance in GBC tissues.
  
  
  RESULTS
  The expression of pStat-3 and integrin αvβ6 were both significantly associated with T stage, lymph node metastasis status, TNM stage (P=0.008, P=0.000, P=0.000 and P=0.036, P=0.001,P=0.000,respectively). IHC and Western blot showed their expressions in GBC tissues were higher than that in paraneoplastic tissues. Moderate positive correlation existed between the two proteins (r =0.349, P <0.001). The survival analysis by Kaplan-Meier and Cox regression model showed that GBC patients with pStat-3 or integrin αvβ6 positive expression had a significantly poorer 2-year survival rate (P = 0.002 and 0.000, the log-rank test, respectively), and either marker could act as unfavorable independent prognostic factors(RR=1.907, P=0.021 and RR=2.046, P=0.038).
  
  
  METHODS
  The expression levels of pStat-3 and integrin αvβ6 were analyzed in GBC cancerous and paraneoplastic tissues of 97 cases via immunohistochemistry(IHC) and further validated by western blot method. Besides, SPSS software was used to observe their clinical significance as well as the two proteins' correlation.
  
  
  CONCLUSIONS
  pStat-3 and integrin αvβ6 were indicators of tumor's progression and poor prognosis of patients with GBC. And the further study involving them may provide a helpful therapeutic target in prevention and treatment of GBC patients.
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Liu
authors.initials: L
authors.lastname: Enyu
authors.affiliation: Department of Dermatology, Shandong Provinical Institute of dermatology and venereology, Jinan 250012, Shandong, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Na
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Zhao
authors.initials: Z
authors.lastname: Chuanzong
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Ben
authors.affiliation: Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Wu
authors.initials: W
authors.lastname: Xiaojuan
authors.affiliation: Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Yan
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.|Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Public Health, Jinan 250012, Shandong, P.R. China.
authors.forename: Li
authors.initials: L
authors.lastname: Zequn
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Hong
authors.initials: H
authors.lastname: Jianguo
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Wang
authors.initials: W
authors.lastname: Jiayong
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.|Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Public Health, Jinan 250012, Shandong, P.R. China.
authors.forename: Liang
authors.initials: L
authors.lastname: Benjia
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Peng
authors.initials: P
authors.lastname: Cheng
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Zhu
authors.initials: Z
authors.lastname: Min
authors.affiliation: Department of General Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, P.R. China.
authors.forename: Zhang
authors.initials: Z
authors.lastname: Zongli
mesh_terms: D000230:Adenocarcinoma / Q000378:metabolism / Q000556:secondary*; D000328:Adult; D000368:Aged; D000951:Antigens, Neoplasm / Q000378:metabolism*; D014408:Biomarkers, Tumor / Q000378:metabolism*; D018196:Carcinoma, Adenosquamous / Q000378:metabolism / Q000556:secondary*; D005260:Female; D005500:Follow-Up Studies; D005706:Gallbladder Neoplasms / Q000378:metabolism / Q000473:pathology*; D006801:Humans; D016023:Integrins / Q000378:metabolism*; D008207:Lymphatic Metastasis; D008297:Male; D008875:Middle Aged; D009361:Neoplasm Invasiveness; D009367:Neoplasm Staging; D010766:Phosphorylation; D011379:Prognosis; D050796:STAT3 Transcription Factor / Q000378:metabolism*; D015996:Survival Rate
chemical_list: D000951:Antigens, Neoplasm; D014408:Biomarkers, Tumor; D016023:Integrins; D050796:STAT3 Transcription Factor; C494086:STAT3 protein, human; C073878:integrin alphavbeta6
keywords: gallbladder cancer; immunohistochemistry; integrin β6; pStat-3; tumorigenesis