title: Isolation and characterization of an HIV-1 envelope glycoprotein-specific B-cell from an immortalized human naïve B-cell library.
abstract: With the recent development of single B-cell cloning techniques, an increasing number of human immunodeficiency virus type 1 (HIV-1)-specific broadly neutralizing antibodies have been isolated since 2009. However, knowledge regarding HIV-1-specific B cells in vivo is limited. In this study, an HIV-1-specific B-cell line was established using healthy PBMC donors by the highly efficient Epstein-Barr virus transformation method to generate immortalized human naïve B-cell libraries. The enrichment of HIV-1 envelope-specific B cells was observed after four rounds of cell panning with the HIV-1 envelope glycoprotein. An HIV-1 envelope-specific stable B-cell line (LCL-P4) was generated. Although this cell line acquired a lymphoblastic phenotype, no expression was observed for activation-induced cytidine deaminase, an enzyme responsible for initiating somatic hypermutation and class switch recombination in B cells. This study describes a method that enables fast isolation of HIV-1-specific B cells, and this approach may extend to isolating other B-cell-specific antigens for further experiments.
authors.affiliation: AIDS Institute, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong, PR China.|Department of Medicine, National Jewish Health, Denver, CO 80206, USA.|Department of Anatomy, The University of Hong Kong, Hong Kong, PR China.
authors.forename: Zehua
authors.initials: Z
authors.lastname: Sun
authors.affiliation: AIDS Institute, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong, PR China.|Department of Biochemistry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong, PR China.
authors.forename: Shiqiang
authors.initials: S
authors.lastname: Lu
authors.affiliation: Liver Disease Institute, Shenzhen Third People's Hospital, Shenzhen 518112, PR China.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Yang
authors.affiliation: AIDS Institute, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong, PR China.
authors.forename: Jingjing
authors.initials: J
authors.lastname: Li
authors.affiliation: AIDS Institute, Department of Microbiology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Pok Fu Lam, Hong Kong, PR China.|Liver Disease Institute, Shenzhen Third People's Hospital, Shenzhen 518112, PR China.
authors.forename: Meiyun
authors.initials: M
authors.lastname: Zhang
mesh_terms: D057134:Antibodies, Neutralizing / Q000378:metabolism*; D001402:B-Lymphocytes / Q000276:immunology*; D002472:Cell Transformation, Viral; D015483:HIV Antibodies / Q000378:metabolism*; D015497:HIV-1 / Q000276:immunology*; D004854:Herpesvirus 4, Human / Q000235:genetics; D006801:Humans; D054299:env Gene Products, Human Immunodeficiency Virus / Q000276:immunology*
chemical_list: D057134:Antibodies, Neutralizing; D015483:HIV Antibodies; D054299:env Gene Products, Human Immunodeficiency Virus