title: A surface tension magnetophoretic device for rare cell isolation and characterization.
abstract: The cancer community continues to search for an efficient and cost-effective technique to isolate and characterize circulating cells (CTCs) as a 'real-time liquid biopsy'. Existing methods to isolate and analyze CTCs require various transfer, wash, and staining steps that can be time consuming, expensive, and led to the loss of rare cells. To overcome the limitations of existing CTC isolation strategies, we have developed an inexpensive 'lab on a chip' device for the enrichment, staining, and analysis of rare cell populations. This device utilizes immunomagnetic positive selection of antibody-bound cells, isolation of cells through an immiscible interface, and filtration. The isolated cells can then be stained utilizing immunofluorescence or used for other downstream detection methods. We describe the construction and initial preclinical testing of the device. Initial tests suggest that the device may be well suited for the isolation of CTCs and could allow the monitoring of cancer progression and the response to therapy over time.
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.|Department of Urology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Emma E
authors.initials: EE
authors.lastname: van der Toom
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.
authors.forename: James E
authors.initials: JE
authors.lastname: Verdone
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.
authors.forename: Changhan
authors.initials: C
authors.lastname: Jun
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.
authors.forename: Doru
authors.initials: D
authors.lastname: Petrisor
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.
authors.forename: Sunghwan
authors.initials: S
authors.lastname: Lim
authors.affiliation: Department of Urology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Jean J M C H
authors.initials: JJ
authors.lastname: de la Rosette
authors.affiliation: Department of Urology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
authors.forename: Theo M
authors.initials: TM
authors.lastname: de Reijke
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.
authors.forename: Michael A
authors.initials: MA
authors.lastname: Gorin
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA.
authors.forename: Kenneth J
authors.initials: KJ
authors.lastname: Pienta
authors.affiliation: Department of Urology, The James Buchanan Brady Urological Institute, School of Medicine, Johns Hopkins University, Baltimore, MD, 21287, USA. dss@jhu.edu.
authors.forename: Dan
authors.identifier: http://orcid.org/0000-0001-6635-9647
authors.initials: D
authors.lastname: Stoianovici
mesh_terms: D045744:Cell Line, Tumor; D006801:Humans; D018189:Immunomagnetic Separation / Q000295:instrumentation* / Q000379:methods*; D056656:Lab-On-A-Chip Devices; D008297:Male; D009360:Neoplastic Cells, Circulating / Q000473:pathology*; D011471:Prostatic Neoplasms / Q000473:pathology; D013499:Surface Properties
keywords: Circulating tumor cells; Magnetophoresis; Micro-fluidic device; Prostate cancer; Rare cells