title: Simultaneous analysis of bisphenol A based compounds and other monomers leaching from resin-based dental materials by UHPLC-MS/MS.
abstract: Resin-based dental materials have raised debates concerning their safety and biocompatibility, resulting in a growing necessity of profound knowledge on the quantity of released compounds into the oral cavity. In this context, the aim of this study was to develop a comprehensive and reliable procedure based on liquid chromatography with mass spectrometry for the simultaneous analysis of various leached compounds (including bisphenol A based compounds) in samples from in vitro experiments. Different experiments were performed to determine the optimal analytical parameters, comprising mass spectrometry parameters, chromatographic separation conditions, and sample preparation. Four internal standards were used as follows: deuterated diethyl phthalate and bisphenol A (commercially available), and deuterated analogues of triethylene glycol dimethacrylate and urethane dimethacrylate (custom-made). The optimized method was validated for linearity of the calibration curves and the associated correlation coefficient, lower limit of quantification, higher limit of quantification, and intra- and interassay accuracy and precision. Additionally, the developed liquid chromatography with tandem mass spectrometry method was applied to the analysis of leaching compounds from four resin-based dental materials. The results indicated that this method is suitable for the analysis of different target compounds leaching from dental materials. This method might serve as a valuable basis for quick and accurate quantification of leached compounds from resin-based dental materials in biological samples.
authors.affiliation: BIOMAT, Department of Oral Health Sciences, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Eveline
authors.initials: E
authors.lastname: Putzeys
authors.affiliation: BIOMAT, Department of Oral Health Sciences, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Stevan M
authors.initials: SM
authors.lastname: Cokic
authors.affiliation: Polymer Chemistry and Materials, Department of Chemistry, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Hui
authors.initials: H
authors.lastname: Chong
authors.affiliation: Polymer Chemistry and Materials, Department of Chemistry, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Mario
authors.initials: M
authors.lastname: Smet
authors.affiliation: Environment and Health, Department of Public Health and Primary Care, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Jeroen
authors.initials: J
authors.lastname: Vanoirbeek
authors.affiliation: Environment and Health, Department of Public Health and Primary Care, KU Leuven (University of Leuven), Leuven, Belgium.|IDEWE, External Service for Prevention and Protection at Work, Heverlee, Belgium.
authors.forename: Lode
authors.initials: L
authors.lastname: Godderis
authors.affiliation: BIOMAT, Department of Oral Health Sciences, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Van Meerbeek
authors.affiliation: BIOMAT, Department of Oral Health Sciences, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Kirsten L
authors.initials: KL
authors.lastname: Van Landuyt
authors.affiliation: Environment and Health, Department of Public Health and Primary Care, KU Leuven (University of Leuven), Leuven, Belgium.
authors.forename: Radu Corneliu
authors.initials: RC
authors.lastname: Duca
mesh_terms: D001559:Benzhydryl Compounds / Q000032:analysis*; D002851:Chromatography, High Pressure Liquid; D003188:Composite Resins / Q000032:analysis*; D003764:Dental Materials / Q000737:chemistry*; D010636:Phenols / Q000032:analysis*; D015203:Reproducibility of Results; D053719:Tandem Mass Spectrometry; D000098826:Bisphenol A Compounds
chemical_list: D001559:Benzhydryl Compounds; D003188:Composite Resins; D003764:Dental Materials; D010636:Phenols; C006780:bisphenol A; D000098826:Bisphenol A Compounds
keywords: dental materials; liquid chromatography; mass spectrometry; monomers; saliva