title: In Situ Growth of Self-Assembled Protein-Polymer Nanovesicles for Enhanced Intracellular Protein Delivery.
abstract: We report a new and general method, in situ growth, for designing self-assembled protein-polymer nanovesicles for intracellular protein delivery.In situ polymerization of a water-soluble monomer from a protein attached with a polymerization initiator yields amphiphilic protein conjugates of a water-insoluble polymer. These conjugates can in situ self-assemble into nanostructures with tunable morphologies from spheres to vesicles. Interestingly, an exogenous protein can be in situ encapsulated inside protein-polymer nanovesicles for enhanced intracellular protein delivery. The in situ growth method may open up new opportunities for designing a variety of self-assembled protein-polymer nanostructures tailored to specific applications.
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Xinyu
authors.initials: X
authors.lastname: Liu
authors.affiliation: Department of Biomedical Engineering, School of Medicine, Tsinghua University , Beijing 100084, P. R. China.
authors.forename: Weiping
authors.identifier: http://orcid.org/0000-0002-2916-3044
authors.initials: W
authors.lastname: Gao
mesh_terms: D053758:Nanoparticles; D049329:Nanostructures; D058105:Polymerization; D011108:Polymers; D011506:Proteins
chemical_list: D011108:Polymers; D011506:Proteins
keywords: nanovesicles; protein delivery,atom transfer radical polymerization; protein−polymer conjugates; self-assembly