title: Use of lignocellulose biomass for endoxylanase production by Streptomyces termitum.
abstract: Actinobacteria isolates from Brazilian Cerrado soil were evaluated for their ability to produce enzymes of the cellulolytic and xylanolytic complex using lignocellulose residual biomass. Preliminary semiquantitative tests, made in Petri plates containing carboxymethylcellulose and beechwood xylan, indicated 11 potential species producing enzymes, all belonging to the genus Streptomyces. The species were subsequently grown in pure substrates in submerged fermentation and analyzed for the production of enzymes endoglucanase, β-glucosidase, endoxylanase, and β-xylosidase. The best results were obtained for endoxylanase enzyme production with Streptomyces termitum(UFLA CES 93). The strain was grown on lignocellulose biomass (bagasse, straw sugarcane, and cocoa pod husk) that was used in natura or acid pretreated. The medium containing sugarcane bagasse in natura favored the production of the endoxylanase that was subsequently optimized through an experimental model. The highest enzyme production 0.387 U mL-1, (25.8 times higher), compared to the lowest value obtained in one of the trials, was observed when combining 2.75% sugar cane bagasse and 1.0 g L-1of yeast extract to the alkaline medium (pH 9.7). This is the first study using S. termitum as a producer of endoxylanase.
authors.affiliation: a Department of Biology , Federal University of Lavras, Campus Universitário , Lavras , Minas Gerais , Brazil.
authors.forename: Alenir Naves
authors.initials: AN
authors.lastname: de Sales
authors.affiliation: a Department of Biology , Federal University of Lavras, Campus Universitário , Lavras , Minas Gerais , Brazil.
authors.forename: Angélica Cristina
authors.initials: AC
authors.lastname: de Souza
authors.affiliation: b Catalysis Division, Biocatalysis Laboratory , National Institute of Technology, Ministry of Science, Technology and Innovation, Rio de Janeiro , Rio de Janeiro , Brazil.|c Department of Biochemistry , Federal University of Rio de Janeiro, Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Rondinele de Oliveira
authors.initials: RO
authors.lastname: Moutta
authors.affiliation: b Catalysis Division, Biocatalysis Laboratory , National Institute of Technology, Ministry of Science, Technology and Innovation, Rio de Janeiro , Rio de Janeiro , Brazil.|c Department of Biochemistry , Federal University of Rio de Janeiro, Rio de Janeiro , Rio de Janeiro , Brazil.
authors.forename: Viridiana Santana
authors.initials: VS
authors.lastname: Ferreira-Leitão
authors.affiliation: a Department of Biology , Federal University of Lavras, Campus Universitário , Lavras , Minas Gerais , Brazil.
authors.forename: Rosane Freitas
authors.initials: RF
authors.lastname: Schwan
authors.affiliation: d Department of Food Science , Federal University of Lavras, Campus Universitário , Lavras , Mines Gerais , Brazil.
authors.forename: Disney Ribeiro
authors.initials: DR
authors.lastname: Dias
mesh_terms: D039903:Actinobacteria / Q000201:enzymology / Q000378:metabolism; D018533:Biomass; D002480:Cellulase / Q000378:metabolism; D002482:Cellulose / Q000378:metabolism; D043364:Endo-1,4-beta Xylanases / Q000378:metabolism*; D007218:Industrial Microbiology / Q000379:methods*; D008031:Lignin / Q000378:metabolism*; D031786:Saccharum / Q000378:metabolism; D013302:Streptomyces / Q000201:enzymology* / Q000378:metabolism; D014990:Xylans / Q000378:metabolism; D014995:Xylosidases / Q000378:metabolism
chemical_list: D014990:Xylans; C036909:lignocellulose; D002482:Cellulose; D008031:Lignin; C027433:bagasse; D014995:Xylosidases; C026579:exo-1,4-beta-D-xylosidase; D002480:Cellulase; D043364:Endo-1,4-beta Xylanases
keywords: Actinobacteria; enzymes; lignocellulosic biomass