title: Effect of incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder on ACE inhibitory activity in fermented milk by L. plantarum LP69.
abstract: BACKGROUND
  Angiotensin I converting enzyme (ACE) plays an important physiological role in regulating hypertension. Lactic acid bacteria are known to produce ACE inhibitory peptides which can lower hypertension during fermentation.
  
  
  METHODS
  The effect of incubation time (0~36 h), inoculum size (3, 4, 5, 6 and 7%, v/v), temperature (25, 30, 35, 40 and 45°C), sterilization time (5, 10, 15, 20 and 25 min), concentration of goat milk powder (8, 10, 12, 14 and 16%, w/v) and whey powder (0.5, 0.6, 0.7, 0.8 and 0.9%, w/v) on ACE inhibitory peptides fermented from goat milk by Lactobacillus plantarum LP69 was investigated using single factor experiment.
  
  
  RESULTS
  The optimal incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder in fermented milk by L. plantarum LP69 was 14 h, 3.0%, 35°C, 20 min, 14% and 0.70% for ACE inhibitory activity and 22 h, 3.0%, 40°C, 25 min, 16% and 0.60% for viable cell counts, respectively.
  
  
  CONCLUSIONS
  The incubation time, inoculum size, temperature, pasteurization time, goat milk powder and whey powder had a significant influence on ACE inhibitory activity in fermented milk by Lactobacillus plantarum LP69, the results are beneficial for further screening of main factors by using fractional factorial designs.
authors.affiliation: College of Life Science and Engineering, Shaanxi University of Science and Technology, Xián, China.
authors.forename: Guowei
authors.initials: G
authors.lastname: Shu
authors.affiliation: College of Life Science and Engineering, Shaanxi University of Science and Technology, Xián, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: College of Life Science and Engineering, Shaanxi University of Science and Technology, Xián, China.
authors.forename: He
authors.initials: H
authors.lastname: Chen
authors.affiliation: College of Life Science and Engineering, Shaanxi University of Science and Technology, Xián, China.
authors.forename: Qiuhong
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: College of Life Science and Engineering, Shaanxi University of Science and Technology, Xián, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Tian
mesh_terms: D000806:Angiotensin-Converting Enzyme Inhibitors / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology*; D000818:Animals; D000959:Antihypertensive Agents / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D001426:Bacterial Proteins / Q000096:biosynthesis / Q000737:chemistry / Q000494:pharmacology*; D002681:China; D043302:Cultured Milk Products / Q000032:analysis* / Q000382:microbiology; D005511:Food Handling; D057141:Food, Preserved / Q000032:analysis* / Q000382:microbiology; D006041:Goats; D006358:Hot Temperature; D048191:Lactiplantibacillus plantarum / Q000254:growth & development* / Q000378:metabolism; D050296:Microbial Viability; D008892:Milk / Q000737:chemistry* / Q000382:microbiology; D061352:Pasteurization; D010446:Peptide Fragments / Q000737:chemistry / Q000378:metabolism / Q000494:pharmacology; D007703:Peptidyl-Dipeptidase A / Q000737:chemistry / Q000378:metabolism; D055550:Protein Stability; D011817:Rabbits; D013997:Time Factors; D000067796:Whey / Q000737:chemistry* / Q000382:microbiology
chemical_list: D000806:Angiotensin-Converting Enzyme Inhibitors; D000959:Antihypertensive Agents; D001426:Bacterial Proteins; D010446:Peptide Fragments; D007703:Peptidyl-Dipeptidase A
keywords: ACE inhibitory peptide; Lactobacillus plantarum; goat milk; incubation time; inoculum size; pasteurization time; whey powder