title: Daboialectin, a C-type lectin from Russell's viper venom induces cytoskeletal damage and apoptosis in human lung cancer cells in vitro.
abstract: 'Daboialectin', a low molecular weight C-type lectin (18.5 kDa) isolated from Russell's viper venom showed cytotoxic effects on human broncho-alveolar carcinoma derived (A549) cell lines. Daboialectin induced inhibition of A549 cell growth was time and concentration dependent with severe morphological changes by altering the functions of small GTPases such as Rac, Rho and cdc42. ROS induced DNA damage may result in apoptosis by inducing disruption of membrane integrity, blebbing and nuclear disintegration by activating caspases. Our results indicate that Daboialectin, a snake c type lectin (Snaclec) isolated from RVV alters morphology of A549 cells via regulation of cytoskeleton through RHO-GTPases. On other hand, the HSP70 and some other anti-apoptotic proteins required for the survival of cancer cells was found to be down-regulated in presence of Daboialectin. Daboialectin was also found to be inhibitory to anti-adhesive and anti-invasive to A549 cells in vitro. Daboialectin is the first Snaclec reported to induce cytoskeletal changes through regulation of RHO-GTPases and blocking anti-apoptotic pathway for a cancer cell line.
authors.affiliation: Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K K Birla Goa Campus, Zuarinagar, Goa, India.
authors.forename: Jigni
authors.initials: J
authors.lastname: Pathan
authors.affiliation: Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K K Birla Goa Campus, Zuarinagar, Goa, India.
authors.forename: Sukanta
authors.initials: S
authors.lastname: Mondal
authors.affiliation: Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K K Birla Goa Campus, Zuarinagar, Goa, India.
authors.forename: Angshuman
authors.initials: A
authors.lastname: Sarkar
authors.affiliation: Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, K K Birla Goa Campus, Zuarinagar, Goa, India. Electronic address: dibakarchakrabarty@goa.bits-pilani.ac.in.
authors.forename: Dibakar
authors.initials: D
authors.lastname: Chakrabarty
mesh_terms: D000072283:A549 Cells; D000818:Animals; D000970:Antineoplastic Agents / Q000494:pharmacology*; D017209:Apoptosis / Q000187:drug effects*; D049109:Cell Proliferation / Q000187:drug effects; D002470:Cell Survival / Q000187:drug effects; D003599:Cytoskeleton / Q000187:drug effects* / Q000648:ultrastructure; D004249:DNA Damage / Q000187:drug effects; D006801:Humans; D037181:Lectins, C-Type; D020559:Monomeric GTP-Binding Proteins / Q000378:metabolism; D017840:Daboia; D014757:Viper Venoms / Q000494:pharmacology*
chemical_list: D000970:Antineoplastic Agents; D037181:Lectins, C-Type; D014757:Viper Venoms; D020559:Monomeric GTP-Binding Proteins
keywords: A549 cells; Apoptosis; Cytotoxicity; Daboialectin; RHO-GTPases; Russell's viper venom