title: Ectopic expression of fungal EcGDH improves nitrogen assimilation and grain yield in rice.
abstract: NADP(H)-dependent glutamate dehydrogenases (GDH) in lower organisms have stronger ammonium affinity than those in higher plants. Here we report that transgenic rice overexpressing the EcGDH from Eurotium cheralieri exhibited significantly enhanced aminating activities. Hydroponic and field tests showed that nitrogen assimilation efficiency and grain yields were markedly increased in these transgenic plants, especially at the low nitrogen conditions. These results suggest that EcGDH may have potential to be used to improve nitrogen assimilation and grain yield in rice.
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, China.|Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Dongying
authors.initials: D
authors.lastname: Tang
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Yuchong
authors.initials: Y
authors.lastname: Peng
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Jianzhong
authors.initials: J
authors.lastname: Lin
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Changqing
authors.initials: C
authors.lastname: Du
authors.affiliation: Academy of Seed Industry of Hunan Yahua, Changsha, 410001, China.
authors.forename: Yuanzhu
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Dan
authors.initials: D
authors.lastname: Wang
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Cong
authors.initials: C
authors.lastname: Liu
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Lu
authors.initials: L
authors.lastname: Yan
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Xiaoying
authors.initials: X
authors.lastname: Zhao
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Li
authors.affiliation: College of Life Sciences, Hunan Normal University, Changsha, 410081, China.
authors.forename: Liangbi
authors.initials: L
authors.lastname: Chen
authors.affiliation: Hunan Province Key Laboratory of Plant Functional Genomics and Developmental Regulation, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, 410082, China.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
mesh_terms: D000066630:Ectopic Gene Expression; D055325:Eurotium / Q000201:enzymology*; D005969:Glutamate Dehydrogenase / Q000235:genetics* / Q000378:metabolism; D009584:Nitrogen / Q000378:metabolism* / Q000494:pharmacology; D012275:Oryza / Q000187:drug effects / Q000235:genetics / Q000378:metabolism*; D030821:Plants, Genetically Modified; D036226:Seedlings / Q000187:drug effects / Q000235:genetics; D012639:Seeds / Q000187:drug effects / Q000254:growth & development*
chemical_list: D005969:Glutamate Dehydrogenase; D009584:Nitrogen