title: An insight into the sialome of Hyalomma excavatum.
abstract: Tick saliva contains hundreds or thousands of proteins that help blood feeding by impairing their hosts' hemostasis, inflammation and immunity. Salivary gland transcriptomes allow the disclosure of this pharmacologically active potion that consists of several multi-gene families, many of which are tick-specific. We here report the "de novo" assembly of ∼138 million reads deriving from a cDNA library from salivary glands of adult male and female Hyalomma excavatum leading to the public deposition of 5337 coding sequences to GenBank. Among the deducted putative secreted proteins, metalloproteases, glycine rich proteins, mucins, anticoagulants of the madanin family and lipocalins were the most expressed. Novel protein families were identified. These sequences will permit proteomic studies aiming at identification of target antigens, epidemiological markers or salivary pharmaceuticals of interest, and contribute to our understanding of the fast evolution of the tick sialome.
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda MD 20852, USA. Electronic address: jribeiro@nih.gov.
authors.forename: José M C
authors.initials: JM
authors.lastname: Ribeiro
authors.affiliation: Institute of Zoology, Slovak Academy of Sciences, Dúbravská cesta 9, 84506, Bratislava, Slovakia. Electronic address: slovak.mirko@gmail.com.
authors.forename: Mirko
authors.initials: M
authors.lastname: Slovák
authors.affiliation: Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, Bethesda MD 20852, USA. Electronic address: ifrancischetti@niaid.nih.gov.
authors.forename: Ivo M B
authors.initials: IM
authors.lastname: Francischetti
mesh_terms: D000818:Animals; D060829:Arthropod Proteins / Q000737:chemistry* / Q000378:metabolism*; D005260:Female; D005786:Gene Expression Regulation; D026863:Ixodidae / Q000502:physiology*; D008297:Male; D012463:Saliva / Q000737:chemistry*; D059467:Transcriptome
chemical_list: D060829:Arthropod Proteins
keywords: Saliva; Salivary gland; Sialome; Tick; Transcriptome