title: The use of regenerated immunoaffinity columns for aflatoxins B1, B2, G1and G2in peanut confection.
abstract: The aim of this study was to investigate the feasibility of using multitime-regenerated immunoaffinity column (IAC) for aflatoxins B1, B2, G1and G2in peanut confection. After each use, the IAC was washed immediately with phosphate-buffered saline and stored for >12h prior to reuse. The evaluation procedure consisted of using extracts of naturallycontaminated peanut confection (4 replicates), aflatoxin-free peanut confection (duplicates), and aflatoxin-free peanut confection sample spiked with the 4 aflatoxins (AFT) at 3 levels in 4 replicates. Each day, 18 test extracts were analyzed using 18 designated IACs. After each use, the IACs were regenerated and reused for corresponding test extracts on the following day. This procedure was repeated daily over the course of 9days. Analytical steps included passing the test extracts through the IACs, washing the columns with water, and eluting AFT with methanol. The eluates were diluted with water and were subjected to reversed phase LC separation, post-column photochemical derivatization and fluorescence detection. After eluting AFT, IACs were immediately regenerated by washing with phosphate buffer solution and storing overnight at 8°C for re-use the following day. Results were analyzed using ANOVA and Tukey tests. The numbers of reuse varied for each AF: For AFB1AFB2, AFG1and AFG2could be reused for 9, 6, 6 and 0 times, respectively. According to AOAC method performance criteria, recoveries ranging from 70% to 125% are considered acceptable at the spiking levels used in this study.
authors.affiliation: Center for Chemical Sciences and Bromatological, Regional Laboratory Centre - Adolfo Lutz Institute of Ribeirão Preto VI, Rua Minas, 877, CEP 14085-410, Ribeirão Preto-SP, Brazil. Electronic address: mhiha@ial.sp.gov.br.
authors.forename: Maria Helena
authors.initials: MH
authors.lastname: Iha
authors.affiliation: Center for Chemical Sciences and Bromatological, Regional Laboratory Centre - Adolfo Lutz Institute of Ribeirão Preto VI, Rua Minas, 877, CEP 14085-410, Ribeirão Preto-SP, Brazil. Electronic address: camilaamini@gmail.com.
authors.forename: Camila Alessandra
authors.initials: CA
authors.lastname: Mini
authors.affiliation: Center for Chemical Sciences and Bromatological, Regional Laboratory Centre - Adolfo Lutz Institute of Ribeirão Preto VI, Rua Minas, 877, CEP 14085-410, Ribeirão Preto-SP, Brazil. Electronic address: Isaura@ial.sp.gov.br.
authors.forename: Isaura Akemi
authors.initials: IA
authors.lastname: Okada
authors.affiliation: Center for Chemical Sciences and Bromatological, Regional Laboratory Centre - Adolfo Lutz Institute of Ribeirão Preto VI, Rua Minas, 877, CEP 14085-410, Ribeirão Preto-SP, Brazil. Electronic address: rcbriganti@ial.sp.gov.br.
authors.forename: Rita de Cássia
authors.initials: RC
authors.lastname: Briganti
authors.affiliation: U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, 5100 Paint Branch Parkway, College Park, MD, USA. Electronic address: mary.trucksess@fda.hhs.gov.
authors.forename: Mary W
authors.initials: MW
authors.lastname: Trucksess
mesh_terms: D016604:Aflatoxin B1 / Q000032:analysis; D000348:Aflatoxins / Q000032:analysis*; D010367:Arachis / Q000737:chemistry*; D002182:Candy / Q000032:analysis*; D002846:Chromatography, Affinity / Q000379:methods*; D002851:Chromatography, High Pressure Liquid
chemical_list: D000348:Aflatoxins; D016604:Aflatoxin B1
keywords: Aflatoxins; Immunoaffinity column; Peanut confection “paçoca”; Regeneration