title: miR-211-5p Suppresses Metastatic Behavior by Targeting SNAI1 in Renal Cancer.
abstract: The Snail family transcriptional repressor 1 (SNAI1) is known to promote metastatic phenotypes in renal cell carcinoma (RCC). However, the mechanism by which SNAI1 promotes RCC metastasis remains largely unexplored. Here, bioinformatics and quantitative validation revealed that miR-211-5p was downregulated in metastatic RCC clinical specimens compared with nonmetastatic RCC tissues. Overexpression of miR-211-5p suppressed RCC cell migration and invasionviadownregulation of SNAI1 expression. Luciferase reporter assays demonstrated that miR-211-5p directly targeted 3'-UTR of SNAI1. Furthermore, miR-211-5p decreased xenograft tumor weight and reducedin vivotumor metastasis in mice. These findings indicate that miR-211-5p-mediated inhibition of SNAIL1 expression contributes to the suppression of RCC progression.Implications:Targeting the miR-211-5p/SNAI1 signaling pathway may be a novel therapeutic approach for the treatment of RCC metastasis.Mol Cancer Res; 15(4); 448-56. ©2017 AACR.
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Kefeng
authors.initials: K
authors.lastname: Wang
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Jin
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Peng
authors.initials: P
authors.lastname: Jin
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Xiang
authors.initials: X
authors.lastname: Fei
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Urology, Shengjing Hospital of China Medical University, Shenyang, China. chenxn80@126.com.
authors.forename: Xiaonan
authors.initials: X
authors.lastname: Chen
mesh_terms: D002292:Carcinoma, Renal Cell / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D019295:Computational Biology / Q000379:methods; D018450:Disease Progression; D015536:Down-Regulation; D015972:Gene Expression Regulation, Neoplastic; D006801:Humans; D007680:Kidney Neoplasms / Q000235:genetics / Q000473:pathology*; D035683:MicroRNAs / Q000235:genetics*; D009361:Neoplasm Invasiveness; D009362:Neoplasm Metastasis; D011401:Promoter Regions, Genetic; D000071250:Snail Family Transcription Factors / Q000235:genetics*
chemical_list: C549405:MIRN211 microRNA, human; D035683:MicroRNAs; C000604407:SNAI1 protein, human; D000071250:Snail Family Transcription Factors