title: Metagenomic analysis of microbial community of a parasitoid waspMegaphragma amalphitanum.
abstract: The vast majority of multicellular organisms coexist with bacterial symbionts that may play various roles during their life cycle. Parasitoid waspMegaphragma amalphitanum(Hymenoptera: Trichogrammatidae) belongs to the smallest known insects whose size is comparable with some bacteria. Using 16S rRNA gene sequencing and Whole Genome Sequencing (WGS), we described microbiota diversity for this arthropod and its potential impact on their lifecycle. Metagenomic sequences were deposited to SRA database which is available at NCBI with accession number SRX2363723 and SRX2363724. We found that small body size and limited lifespan do not lead to a significant reduction of bacterial symbionts diversity. At the same time, we show here a specific feature of microbiota composition inM. amalphitanum- the absence of the Rickettsiaceae family representatives that are known to cause sex-ratio distortion in arthropods and well represented in other populations of parasitoid wasps.
authors.affiliation: National Research Centre "Kurchatov Institute", Russian Federation.
authors.forename: A V
authors.initials: AV
authors.lastname: Nedoluzhko
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation.
authors.forename: F S
authors.initials: FS
authors.lastname: Sharko
authors.affiliation: National Research Centre "Kurchatov Institute", Russian Federation.
authors.forename: S V
authors.initials: SV
authors.lastname: Tsygankova
authors.affiliation: National Research Centre "Kurchatov Institute", Russian Federation.
authors.forename: E S
authors.initials: ES
authors.lastname: Boulygina
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation.
authors.forename: A S
authors.initials: AS
authors.lastname: Sokolov
authors.affiliation: National Research Centre "Kurchatov Institute", Russian Federation.
authors.forename: S M
authors.initials: SM
authors.lastname: Rastorguev
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation.
authors.forename: V V
authors.initials: VV
authors.lastname: Kadnikov
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation.
authors.forename: A V
authors.initials: AV
authors.lastname: Mardanov
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation; Lomonosov Moscow State University, Faculty of Biology, Russian Federation.
authors.forename: N V
authors.initials: NV
authors.lastname: Ravin
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation.
authors.forename: A M
authors.initials: AM
authors.lastname: Mazur
authors.affiliation: Lomonosov Moscow State University, Faculty of Biology, Russian Federation.
authors.forename: A A
authors.initials: AA
authors.lastname: Polilov
authors.affiliation: National Research Centre "Kurchatov Institute", Russian Federation.
authors.forename: N M
authors.initials: NM
authors.lastname: Gruzdeva
authors.affiliation: Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation; Lomonosov Moscow State University, Faculty of Biology, Russian Federation.
authors.forename: E B
authors.initials: EB
authors.lastname: Prokhortchouk
authors.affiliation: National Research Centre "Kurchatov Institute", Russian Federation; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Russian Federation; Lomonosov Moscow State University, Faculty of Biology, Russian Federation.
authors.forename: K G
authors.initials: KG
authors.lastname: Skryabin