title: Combined Autoradiography and Formaldehyde-induced Fluorescence Methods for Localization of Radioactively Labeled Substances in Relation to Monoamine Neurons1.
abstract: A combined technique of formaldehyde-induced fluorescence (FIF) and autoradiography is described for the localization of radioactively labeled substances in relation to monoamine neurons. This method permits the simultaneous visualization of 3H-labeled steroid hormone or drug uptake sites and fluorescing monoamine neural elements (cell bodies, fiber projections, terminals) in the same tissue section. Thin frozen sections cut in a cryostat are freeze-dried, exposed to formaldehyde vapor at 80°C, and carried through dry-mount autoradiography processing steps before fluorescence microscopy screening. Subsequent histological staining of sections and light microscopy are employed for conventional autoradiogram screening. With this procedure, 3H-estradiol and 3H-dihydro-testosterone are localized in various catecholamine (CA) neurons in the diencephalon and lower brain stem of the rat. Also, catecholaminergic as well as noncatechol-aminergic sex steroid target neurons are seen to be innervated by CA terminals in various rat brain regions.
authors.affiliation: Department of Anatomy and Biological Sciences Research Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27514.
authors.forename: Lester D
authors.initials: LD
authors.lastname: Grant
authors.affiliation: Department of Anatomy and Biological Sciences Research Center, University of North Carolina School of Medicine, Chapel Hill, North Carolina 27514.
authors.forename: Walter E
authors.initials: WE
authors.lastname: Stumpf
keywords: Androgen; Autoradiography; Catecholamine; Combined histochemical methods.; Estrogen; Formaldehyde-induced fluorescence; Steroid hormone