title: Rapid Classification of Turmeric Based on DNA Fingerprint by Near-Infrared Spectroscopy Combined with Moving Window Partial Least Squares-Discrimination Analysis.
abstract: In this research, near-infrared (NIR) spectroscopy in combination with moving window partial least squares-discrimination analysis (MWPLS-DA) was utilized to discriminate the variety of turmeric based on DNA markers, which correlated to the quantity of curcuminoid. Curcuminoid was used as a marker compound in variety identification due to the most pharmacological properties of turmeric possessed from it. MWPLS-DA optimized informative NIR spectral regions for the fitting and prediction to {-1/1}-coded turmeric varieties, indicating variables in the development of latent variables in discrimination analysis. Consequently, MWPLS-DA benefited in the selection of combined informative NIR spectral regions of 1100 - 1260, 1300 - 1500 and 1880 - 2500 nm for classification modeling of turmeric variety with 148 calibration samples, and yielded the results better than that obtained from a partial least squares-discrimination analysis (PLS-DA) model built by using the whole NIR spectral region. An effective and rapid strategy of using NIR in combination with MWPLS-DA provided the best variety identification results of 100% in both specificity and total accuracy for 48 test samples.
authors.affiliation: Special Research Unit of Non-destructive Quality Evaluation of Commodities, Kasetsart Agricultural and Agro-Industrial Product Improvement Institute, Kasetsart University.
authors.forename: Sumaporn
authors.initials: S
authors.lastname: Kasemsumran
authors.forename: Nattaporn
authors.initials: N
authors.lastname: Suttiwijitpukdee
authors.forename: Vichein
authors.initials: V
authors.lastname: Keeratinijakal
mesh_terms: D030024:Curcuma / Q000145:classification* / Q000235:genetics*; D016172:DNA Fingerprinting; D016002:Discriminant Analysis; D016018:Least-Squares Analysis; D019265:Spectroscopy, Near-Infrared; D013997:Time Factors