title: Preparation of cellulase concoction using differential adsorption phenomenon.
abstract: Controlled depolymerization of cellulose is essential for the production of valuable cellooligosaccharides and cellobiose from lignocellulosic biomass. However, enzymatic cellulose hydrolysis involves multiple synergistically acting enzymes, making difficult to control the depolymerization process and generate desired product. This work exploits the varying adsorption properties of the cellulase components to the cellulosic substrate and aims to control the enzyme activity. Cellulase adsorption was favored on pretreated cellulosic biomass as compared to synthetic cellulose. Preferential adsorption of exocellulases was observed over endocellulase, while β-glucosidases remained unadsorbed. Adsorbed enzyme fraction with bound exocellulases when used for hydrolysis generated cellobiose predominantly, while the unadsorbed enzymes in the liquid fraction produced cellooligosaccharides majorly, owing to its high endocellulases activity. Thus, the differential adsorption phenomenon of the cellulase components can be used for the controlling cellulose hydrolysis for the production of an array of sugars.
authors.affiliation: a DBT-ICT Centre of Energy Biosciences , Institute of Chemical Technology, Nathalal Parikh Marg, Matunga , Mumbai , Maharashtra , India.
authors.forename: Sachinkumar
authors.initials: S
authors.lastname: Birhade
authors.affiliation: a DBT-ICT Centre of Energy Biosciences , Institute of Chemical Technology, Nathalal Parikh Marg, Matunga , Mumbai , Maharashtra , India.
authors.forename: Mukesh
authors.initials: M
authors.lastname: Pednekar
authors.affiliation: a DBT-ICT Centre of Energy Biosciences , Institute of Chemical Technology, Nathalal Parikh Marg, Matunga , Mumbai , Maharashtra , India.
authors.forename: Shilpa
authors.initials: S
authors.lastname: Sagwal
authors.affiliation: a DBT-ICT Centre of Energy Biosciences , Institute of Chemical Technology, Nathalal Parikh Marg, Matunga , Mumbai , Maharashtra , India.
authors.forename: Annamma
authors.initials: A
authors.lastname: Odaneth
authors.affiliation: a DBT-ICT Centre of Energy Biosciences , Institute of Chemical Technology, Nathalal Parikh Marg, Matunga , Mumbai , Maharashtra , India.
authors.forename: Arvind
authors.initials: A
authors.lastname: Lali
mesh_terms: D000327:Adsorption; D018533:Biomass; D001709:Biotechnology; D002475:Cellobiose / Q000378:metabolism; D002480:Cellulase / Q000302:isolation & purification / Q000378:metabolism*; D002482:Cellulose / Q000378:metabolism*; D004789:Enzyme Activation; D004800:Enzymes, Immobilized / Q000302:isolation & purification / Q000378:metabolism; D006868:Hydrolysis; D014908:Triticum / Q000378:metabolism
chemical_list: D004800:Enzymes, Immobilized; D002475:Cellobiose; D002482:Cellulose; D002480:Cellulase
keywords: Cellooligosaccharides; cellulase; cellulose hydrolysis; controlled depolymerization; differential adsorption; exocellulases