title: MicroRNA-155 is upregulated in ascites in patients with spontaneous bacterial peritonitis.
abstract: MircoRNA's (miR) have been recognised as important modulators of gene expression and potential biomarkers. However, they have been rarely investigated in bio fluids apart from blood. We investigated the association of miR-125b and miR-155 with complications of cirrhosis. Ascites was prospectively collected from patients with cirrhosis undergoing paracentesis at our department. miR's were determined in the supernatant using qPCR and normalized by SV-40. Clinical parameters were assessed at paracentesis and during follow-up. 76 specimens from 72 patients were analysed. MiR's were not associated to age, sex or aetiology of cirrhosis. MiR-125b levels differed between patients with low and high MELD score, and miR-125b levels showed an inverse correlation to serum creatinine (r2= -0.23; p = 0.05). MiR-155 was elevated in patients with spontaneous bacterial peritonitis (SBP) (n = 10; p = 0.04). MiR-155 levels differed between patients with and without 30-day survival (p = 0.02). No association of ascites levels of investigated miR's to size of varices, episodes of gastrointestinal bleeding or hepatorenal syndrome was observed. While miR-125b levels in ascites seem to be associated with liver and renal dysfunction, miR-155 might be implicated in local immune response in SBP.
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.|German Center for Infection Research, Partner site Bonn-Cologne, Bonn, Germany.
authors.forename: Philipp
authors.initials: P
authors.lastname: Lutz
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.
authors.forename: Mohamed
authors.initials: M
authors.lastname: M Haimid
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Pohlmann
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.
authors.forename: Jennifer
authors.initials: J
authors.lastname: Lehmann
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.
authors.forename: Christian
authors.initials: C
authors.lastname: Jansen
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.
authors.forename: Robert
authors.initials: R
authors.lastname: Schierwagen
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.
authors.forename: Sabine
authors.initials: S
authors.lastname: Klein
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.|German Center for Infection Research, Partner site Bonn-Cologne, Bonn, Germany.
authors.forename: Christian P
authors.initials: CP
authors.lastname: Strassburg
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.|German Center for Infection Research, Partner site Bonn-Cologne, Bonn, Germany.
authors.forename: Ulrich
authors.initials: U
authors.lastname: Spengler
authors.affiliation: Department of Internal Medicine I, University of Bonn, Sigmund-Freud-Strasse 25, 53127 Bonn, Germany.|Institute of Clinical Research, Faculty of Health Sciences, University of Southern Denmark, Campusvej 55, DK- 5230 Odense M, Denmark.
authors.forename: Jonel
authors.initials: J
authors.lastname: Trebicka
mesh_terms: D000368:Aged; D001201:Ascites / Q000097:blood / Q000150:complications / Q000235:genetics* / Q000382:microbiology*; D001424:Bacterial Infections / Q000097:blood / Q000150:complications / Q000235:genetics* / Q000382:microbiology*; D015415:Biomarkers / Q000097:blood; D015331:Cohort Studies; D003404:Creatinine / Q000097:blood; D004932:Esophageal and Gastric Varices / Q000097:blood / Q000150:complications / Q000235:genetics / Q000382:microbiology; D005260:Female; D006530:Hepatorenal Syndrome / Q000097:blood / Q000150:complications / Q000382:microbiology; D006801:Humans; D007249:Inflammation / Q000097:blood / Q000150:complications / Q000473:pathology; D008113:Liver Neoplasms / Q000150:complications / Q000473:pathology; D008297:Male; D035683:MicroRNAs / Q000235:genetics* / Q000378:metabolism; D008875:Middle Aged; D010538:Peritonitis / Q000097:blood / Q000150:complications / Q000235:genetics* / Q000382:microbiology*; D016019:Survival Analysis; D015854:Up-Regulation / Q000235:genetics*
chemical_list: D015415:Biomarkers; C524942:MIRN155 microRNA, human; D035683:MicroRNAs; D003404:Creatinine