title: Complete genome sequence and expression profile of the commercial lytic enzyme producer Lysobacter enzymogenes M497-1.
abstract: Lysobacter enzymogenes M497-1 is a producer of commercialized achromopeptidase and is expected to harbour genes encoding various other antimicrobial enzymes. Here, we present the complete sequence of the genome of M497-1 and the expression profiles of the genes for various antimicrobial enzymes. Of the 117 peptidase-encoding genes found in the 6.1-Mb genome of M497-1, 15 genes (aside from the gene encoding the achromopeptidase) were expressed at a level higher than that of the average ribosomal protein genes in the 24-h culture. Thus, the strain was found more valuable than hitherto considered. In addition, M497-1 harbours 98 genes involved in the biosynthesis of various natural products, 16 of which are M497-1-specific across 4 Lysobacter species. A gene cluster starting at LEN_2603 through LEN_2673 among the 98 genes closely resembled the lysobactin biosynthesis gene cluster of Lysobacter sp. ATCC 53042. It is likely that M497-1 may produce lysobactin or related antibacterial compounds. Furthermore, comparative genomic analysis of M497-1 and four other Lysobacter species revealed that their core genome structure comprises 3,737 orthologous groups. Our findings are expected to advance further biotechnological application of Lysobacter spp. as a promising source of natural bioactive compounds.
authors.affiliation: Microbial Genome Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokohama, Kanagawa, Japan.
authors.forename: Hideto
authors.initials: H
authors.lastname: Takami
authors.affiliation: Comparative Genomics Laboratory, National Institute of Genetics, Mishima, Japan.
authors.forename: Atsushi
authors.initials: A
authors.lastname: Toyoda
authors.affiliation: Laboratory of Genome Informatics, National Institute for Basic Biology, National Institutes of Natural Sciences, Aichi, Japan.
authors.forename: Ikuo
authors.initials: I
authors.lastname: Uchiyama
authors.affiliation: Graduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, Meguro-ku, Tokyo, Japan.
authors.forename: Takehiko
authors.initials: T
authors.lastname: Itoh
authors.affiliation: Microbial Genome Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokohama, Kanagawa, Japan.
authors.forename: Yoshihiro
authors.initials: Y
authors.lastname: Takaki
authors.affiliation: Microbial Genome Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokohama, Kanagawa, Japan.
authors.forename: Wataru
authors.initials: W
authors.lastname: Arai
authors.affiliation: Microbial Genome Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokohama, Kanagawa, Japan.
authors.forename: Shinro
authors.initials: S
authors.lastname: Nishi
authors.affiliation: Microbial Genome Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), Yokohama, Kanagawa, Japan.
authors.forename: Mikihiko
authors.initials: M
authors.lastname: Kawai
authors.affiliation: National Institute of Advanced Industrial Science and Technology, Koto-ku, Tokyo, Japan.
authors.forename: Kazuo
authors.initials: K
authors.lastname: Shin-Ya
authors.affiliation: Kitasato Institute for Life Sciences, Kitasato University, Sagamihara, Kanagawa, Japan.
authors.forename: Haruo
authors.initials: H
authors.lastname: Ikeda
mesh_terms: D001483:Base Sequence; D047630:Depsipeptides / Q000096:biosynthesis; D015964:Gene Expression Regulation, Bacterial; D059646:Genome Size; D016680:Genome, Bacterial; D023281:Genomics; D053528:Lysobacter / Q000235:genetics*; D017423:Sequence Analysis, RNA; D059467:Transcriptome; D000073336:Whole Genome Sequencing
chemical_list: D047630:Depsipeptides; C056790:katanosin B
keywords: Lysobacter enzymogenes; RNA sequencing; genome sequence; lytic enzyme; metabolic potential