title: Physiological Remediation of Cobalt Ferrite Nanoparticles by Ferritin.
abstract: Metallic nanoparticles have been increasingly suggested as prospective therapeutic nanoplatforms, yet their long-term fate and cellular processing in the body is poorly understood. Here we examined the role of an endogenous iron storage protein - namely the ferritin - in the remediation of biodegradable cobalt ferrite magnetic nanoparticles. Structural and elemental analysis of ferritins close to exogenous nanoparticles within spleens and livers of mice injected in vivo with cobalt ferrite nanoparticles, suggests the intracellular transfer of degradation-derived cobalt and iron, entrapped within endogenous protein cages. In addition, the capacity of ferritin cages to accommodate and store the degradation products of cobalt ferrite nanoparticles was investigated in vitro in the acidic environment mimicking the physiological conditions that are present within the lysosomes. The magnetic, colloidal and structural follow-up of nanoparticles and proteins in the lysosome-like medium confirmed the efficient remediation of nanoparticle-released cobalt and iron ions by ferritins in solution. Metal transfer into ferritins could represent a quintessential process in which biomolecules and homeostasis regulate the local degradation of nanoparticles and recycle their by-products.
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Volatron
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.|Inserm U970, Paris Cardiovascular Research Center-PARCC/Université Paris-Descartes, Sorbonne Paris Cité, 56 rue Leblanc, 75015 France.
authors.forename: Jelena
authors.initials: J
authors.lastname: Kolosnjaj-Tabi
authors.affiliation: Laboratoire Matériaux et Phénomènes Quantiques, UMR 7162 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.|Department of Physics, University of Agriculture Faisalabad, Pakistan.
authors.forename: Yasir
authors.initials: Y
authors.lastname: Javed
authors.affiliation: Service de Physique Biomédicale, Université de Mons, 20 Place du Parc, 7000 Mons, Belgium.
authors.forename: Quoc Lam
authors.initials: QL
authors.lastname: Vuong
authors.affiliation: Service de Physique Biomédicale, Université de Mons, 20 Place du Parc, 7000 Mons, Belgium.
authors.forename: Yves
authors.initials: Y
authors.lastname: Gossuin
authors.affiliation: Laboratoire PHENIX, UMR 7195, CNRS/Université Pierre et Marie Curie/ESPCI, 4 place Jussieu, 75252 Paris Cedex 05, France.
authors.forename: Sophie
authors.initials: S
authors.lastname: Neveu
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Luciani
authors.affiliation: ITODYS, Interfaces, Traitements, Organisation et Dynamique des Systèmes, UMR 7086 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, 75205 Paris Cedex 13, France.
authors.forename: Miryana
authors.initials: M
authors.lastname: Hémadi
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Carn
authors.affiliation: Laboratoire Matériaux et Phénomènes Quantiques, UMR 7162 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Alloyeau
authors.affiliation: Laboratoire Matière et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205 Paris Cedex 13, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Gazeau