title: Mapping and Genetic Structure Analysis of the Anthracnose Resistance Locus Co-1HY in the Common Bean (Phaseolus vulgaris L.).
abstract: Anthracnose is a destructive disease of the common bean (Phaseolus vulgaris L.). The Andean cultivar Hongyundou has been demonstrated to possess strong resistance to anthracnose race 81. To study the genetics of this resistance, the Hongyundou cultivar was crossed with a susceptible genotype Jingdou. Segregation of resistance for race 81 was assessed in the F2 population and F2:3 lines under controlled conditions. Results indicate that Hongyundou carries a single dominant gene for anthracnose resistance. An allele test by crossing Hongyundou with another resistant cultivar revealed that the resistance gene is in the Co-1 locus (therefore named Co-1HY). The physical distance between this locus and the two flanking markers was 46 kb, and this region included four candidate genes, namely, Phvul.001G243500, Phvul.001G243600, Phvul.001G243700 and Phvul.001G243800. These candidate genes encoded serine/threonine-protein kinases. Expression analysis of the four candidate genes in the resistant and susceptible cultivars under control condition and inoculated treatment revealed that all the four candidate genes are expressed at significantly higher levels in the resistant genotype than in susceptible genotype. Phvul.001G243600 and Phvul.001G243700 are expressed nearly 15-fold and 90-fold higher in the resistant genotype than in the susceptible parent before inoculation, respectively. Four candidate genes will provide useful information for further research into the resistance mechanism of anthracnose in common bean. The closely linked flanking markers identified here may be useful for transferring the resistance allele Co-1HY from Hongyundou to elite anthracnose susceptible common bean lines.
authors.affiliation: Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture; The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, China.|Tobacco Research Institute, Chinese Academy of Agricultural Science, Qingdao, Shandong, China.
authors.forename: Mingli
authors.initials: M
authors.lastname: Chen
authors.affiliation: Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture; The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Wu
authors.affiliation: Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture; The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Lanfen
authors.initials: L
authors.lastname: Wang
authors.affiliation: RMIT University, School of Science, Melbourne, Victoria, Australia.
authors.forename: Nitin
authors.initials: N
authors.lastname: Mantri
authors.affiliation: Qingdao Academy of Agricultural Sciences, Shandong, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Zhang
authors.affiliation: Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture; The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Zhendong
authors.initials: Z
authors.lastname: Zhu
authors.affiliation: Key Laboratory of Crop Germplasm Resources and Utilization, Ministry of Agriculture; The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, the Chinese Academy of Agricultural Sciences, Beijing, China.
authors.forename: Shumin
authors.initials: S
authors.lastname: Wang
mesh_terms: D002874:Chromosome Mapping; D020231:Colletotrichum / Q000472:pathogenicity*; D060467:Disease Resistance / Q000235:genetics*; D017343:Genes, Plant; D056726:Genetic Association Studies; D008040:Genetic Linkage; D056426:Genetic Loci; D005819:Genetic Markers; D027805:Phaseolus / Q000235:genetics* / Q000276:immunology*; D010935:Plant Diseases / Q000235:genetics / Q000276:immunology*; D011110:Polymorphism, Genetic
chemical_list: D005819:Genetic Markers