title: The Prognostic Role of SIRT1-Autophagy Axis in Gastric Cancer.
abstract: Aim. Sirtuin 1 (SIRT1) can induce autophagy through deacetylation of Beclin-1 and other autophagy mediators. However, the relationship between SIRT1 and autophagy in GC has not been defined. Therefore, the aim of this study was to confirm the prognostic value of SIRT1 and Beclin-1 and their relationship in GC patients.Methods. Transmission electron microscopy (TEM) was performed to examine the autophagy in GC patients. Immunohistochemistry was used to examine the expression of SIRT1, Beclin-1 in GC, and adjacent nonneoplastic mucosa.Results. In 7 out of 8 GC patients' samples examined by TEM, more autophagic vesicles were observed in GC tissues compared to adjacent nonneoplastic mucosa tissue. A positive correlation between SIRT1 and Beclin-1 expression was observed. Furthermore, Beclin-1 or SIRT1 expression alone or their combined expression were significantly correlated with advanced clinicopathological parameters. High Beclin-1 and SIRT1 expression alone and their combined high expression predicted shorter overall survival and relapse-free survival. Both high Beclin-1 and SIRT1 expressions were independent prognostic factors for poor survival of GC.Conclusions. Based on our results we can conclude that SIRT1 and Beclin-1 expression alone or in combination can be used as prognostic indicator and may represent new therapeutic targets in GC.
authors.affiliation: Department of General Surgery, The First Affiliated Hospital Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Guanglin
authors.initials: G
authors.lastname: Qiu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xuqi
authors.identifier: 0000-0002-2497-020X
authors.initials: X
authors.lastname: Li
authors.affiliation: Xi'an Health School, Xi'an 710054, China.
authors.forename: Chao
authors.identifier: 0000-0002-2560-7517
authors.initials: C
authors.lastname: Wei
authors.affiliation: Department of General Surgery, The First Affiliated Hospital Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Xiangming
authors.identifier: 0000-0002-3730-7802
authors.initials: X
authors.lastname: Che
authors.affiliation: Department of General Surgery, The First Affiliated Hospital Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Shicai
authors.identifier: 0000-0001-5313-7711
authors.initials: S
authors.lastname: He
authors.affiliation: Department of General Surgery, The First Affiliated Hospital Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Jing
authors.identifier: 0000-0001-8938-006X
authors.initials: J
authors.lastname: Lu
authors.affiliation: Department of General Surgery, The First Affiliated Hospital Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Shufeng
authors.identifier: 0000-0001-9166-511X
authors.initials: S
authors.lastname: Wang
authors.affiliation: Shaanxi Friendship Hospital, Xi'an 710068, China.
authors.forename: Ke
authors.identifier: 0000-0001-5681-8635
authors.initials: K
authors.lastname: Pang
authors.affiliation: Department of General Surgery, The First Affiliated Hospital Medical College of Xi'an Jiaotong University, Xi'an 710061, China.
authors.forename: Lin
authors.identifier: 0000-0001-8260-1886
authors.initials: L
authors.lastname: Fan
mesh_terms: D000328:Adult; D000368:Aged; D001343:Autophagy; D000071186:Beclin-1 / Q000235:genetics / Q000378:metabolism; D014408:Biomarkers, Tumor / Q000235:genetics / Q000378:metabolism*; D005260:Female; D006801:Humans; D007413:Intestinal Mucosa / Q000378:metabolism / Q000648:ultrastructure; D008297:Male; D008875:Middle Aged; D056564:Sirtuin 1 / Q000235:genetics / Q000378:metabolism*; D013274:Stomach Neoplasms / Q000378:metabolism* / Q000473:pathology
chemical_list: C491997:BECN1 protein, human; D000071186:Beclin-1; D014408:Biomarkers, Tumor; C447939:SIRT1 protein, human; D056564:Sirtuin 1