title: Dietary overload lithium decreases the adipogenesis in abdominal adipose tissue of broiler chickens.
abstract: To investigate the toxic effects of dietary overload lithium on the adipogenesis in adipose tissue of chicken and the role of hypothalamic neuropeptide Y (NPY) in this process, one-day-old male chicks were fed with the basal diet added with 0 (control) or 100mg lithium/kg diet from lithium chloride (overload lithium) for 35days. Abdominal adipose tissue and hypothalamus were collected at day 6, 14, and 35. As a percentage of body weight, abdominal fat decreased (p<0.001) at day 6, 14, and 35, and feed intake and body weight gain decreased during day 7-14, and day 15-35 in overload lithium treated broilers as compared to control. Adipocyte diameter and DNA content in abdominal adipose tissue were significantly lower in overload-lithium treatment than control at day 35, although no significant differences were observed at day 6 and 14. Dietary overload lithium decreased (p<0.01) transcriptional expression of preadipocyte proliferation makers ki-67 (KI67), microtubule-associated protein homolog (TPX2), and topoisomerase 2-alpha (TOP2A), and preadipocyte differentiation transcriptional factors peroxisome proliferator-activated receptor-γ (PPARγ), and CCAAT/enhancer binding protein (C/EBP) α mRNA abundance in abdominal adipose tissue. In hypothalamus, dietary overload lithium influenced (p<0.001) NPY, and NPY receptor (NPYR) 6 mRNA abundance at day 6 and 14, but not at day 35. In conclusion, dietary overload lithium decreased the adipogenesis in abdominal adipose tissue of chicken, which was accompanied by depressing transcriptional expression of adipogenesis-associated factors. Hypothalamic NPY had a potential role in the adipogenesis in abdominal adipose tissue of broilers with a short-term overload lithium treatment.
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China. Electronic address: shipingbai@sicau.edu.cn.
authors.forename: Shiping
authors.initials: S
authors.lastname: Bai
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Shuqin
authors.initials: S
authors.lastname: Pan
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Keying
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Xuemei
authors.initials: X
authors.lastname: Ding
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Jianping
authors.initials: J
authors.lastname: Wang
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Qiufeng
authors.initials: Q
authors.lastname: Zeng
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Yue
authors.initials: Y
authors.lastname: Xuan
authors.affiliation: Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, Sichuan, China.
authors.forename: Zuowei
authors.initials: Z
authors.lastname: Su
mesh_terms: D050153:Abdominal Fat / Q000187:drug effects* / Q000378:metabolism; D050156:Adipogenesis / Q000187:drug effects*; D000818:Animals; D002645:Chickens; D004247:DNA / Q000378:metabolism; D004032:Diet; D005786:Gene Expression Regulation / Q000187:drug effects; D005993:Glycerolphosphate Dehydrogenase / Q000378:metabolism; D007031:Hypothalamus / Q000187:drug effects / Q000378:metabolism; D018021:Lithium Chloride / Q000633:toxicity*; D008297:Male; D009478:Neuropeptide Y / Q000378:metabolism; D012333:RNA, Messenger / Q000378:metabolism; D017476:Receptors, Neuropeptide Y / Q000235:genetics; D059467:Transcriptome
chemical_list: D009478:Neuropeptide Y; D012333:RNA, Messenger; D017476:Receptors, Neuropeptide Y; C109492:neuropeptide Y6 receptor; D004247:DNA; D005993:Glycerolphosphate Dehydrogenase; D018021:Lithium Chloride
keywords: Adipocyte cellularity; Adipose tissue; Broiler; Lithium; Neuropeptide Y