title: Urinary1H Nuclear Magnetic Resonance Metabolomic Fingerprinting Reveals Biomarkers of Pulse Consumption Related to Energy-Metabolism Modulation in a Subcohort from the PREDIMED study.
abstract: Little is known about the metabolome fingerprint of pulse consumption. The study of robust and accurate biomarkers for pulse dietary assessment has great value for nutritional epidemiology regarding health benefits and their mechanisms. To characterize the fingerprinting of dietary pulses (chickpeas, lentils, and beans), spot urine samples from a subcohort from the PREDIMED study were stratified using a validated food frequency questionnaire. Urine samples of nonpulse consumers (≤4 g/day of pulse intake) and habitual pulse consumers (≥25 g/day of pulse intake) were analyzed using a1H nuclear magnetic resonance (NMR) metabolomics approach combined with multi- and univariate data analysis. Pulse consumption showed differences through 16 metabolites coming from (i) choline metabolism, (ii) protein-related compounds, and (iii) energy metabolism (including lower urinary glucose). Stepwise logistic regression analysis was applied to design a combined model of pulse exposure, which resulted in glutamine, dimethylamine, and 3-methylhistidine. This model was evaluated by a receiver operating characteristic curve (AUC > 90% in both training and validation sets). The application of NMR-based metabolomics to reported pulse exposure highlighted new candidates for biomarkers of pulse consumption and the impact on energy metabolism, generating new hypotheses on energy modulation. Further intervention studies will confirm these findings.
authors.affiliation: Biomarkers & Nutrimetabolomics Laboratory, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA, Campus Torribera, Faculty of Pharmacy and Food Science, University of Barcelona , Barcelona 08028, Spain.
authors.forename: Francisco
authors.initials: F
authors.lastname: Madrid-Gambin
authors.affiliation: Biomarkers & Nutrimetabolomics Laboratory, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA, Campus Torribera, Faculty of Pharmacy and Food Science, University of Barcelona , Barcelona 08028, Spain.|CIBER Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III , Barcelona 08028, Spain.
authors.forename: Rafael
authors.initials: R
authors.lastname: Llorach
authors.affiliation: Biomarkers & Nutrimetabolomics Laboratory, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA, Campus Torribera, Faculty of Pharmacy and Food Science, University of Barcelona , Barcelona 08028, Spain.|Department of Biological Sciences and Department of Computing Science, University of Alberta , Edmonton AB T6G 2E9, Canada.
authors.forename: Rosa
authors.initials: R
authors.lastname: Vázquez-Fresno
authors.affiliation: Biomarkers & Nutrimetabolomics Laboratory, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA, Campus Torribera, Faculty of Pharmacy and Food Science, University of Barcelona , Barcelona 08028, Spain.|CIBER Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III , Barcelona 08028, Spain.
authors.forename: Mireia
authors.initials: M
authors.lastname: Urpi-Sarda
authors.affiliation: Biomarkers & Nutrimetabolomics Laboratory, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA, Campus Torribera, Faculty of Pharmacy and Food Science, University of Barcelona , Barcelona 08028, Spain.
authors.forename: Enrique
authors.initials: E
authors.lastname: Almanza-Aguilera
authors.affiliation: Biomarkers & Nutrimetabolomics Laboratory, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA, Campus Torribera, Faculty of Pharmacy and Food Science, University of Barcelona , Barcelona 08028, Spain.|CIBER Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III , Barcelona 08028, Spain.
authors.forename: Mar
authors.initials: M
authors.lastname: Garcia-Aloy
authors.affiliation: Department of Internal Medicine, Hospital Clinic, Institut d'Investigacions Biomèdiques August Pi Sunyer (IDIBAPS) , Barcelona 08036, Spain.|CIBER OBN, The Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition, Instituto de Salud Carlos III , Madrid 28029, Spain.
authors.forename: Ramon
authors.initials: R
authors.lastname: Estruch
authors.affiliation: CIBER OBN, The Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition, Instituto de Salud Carlos III , Madrid 28029, Spain.|Department of Preventive Medicine and Public Health, University of Valencia , Valencia 46010, Spain.
authors.forename: Dolores
authors.initials: D
authors.lastname: Corella
authors.affiliation: Biomarkers & Nutrimetabolomics Laboratory, Nutrition, Food Science and Gastronomy Department, XaRTA, INSA, Campus Torribera, Faculty of Pharmacy and Food Science, University of Barcelona , Barcelona 08028, Spain.|CIBER Fragilidad y Envejecimiento Saludable (CIBERFES), Instituto de Salud Carlos III , Barcelona 08028, Spain.
authors.forename: Cristina
authors.identifier: 0000-0002-8494-4978
authors.initials: C
authors.lastname: Andres-Lacueva
mesh_terms: D015415:Biomarkers / Q000378:metabolism*; D004032:Diet; D004734:Energy Metabolism / Q000235:genetics*; D007887:Fabaceae / Q000378:metabolism; D006801:Humans; D009682:Magnetic Resonance Spectroscopy / Q000379:methods*; D055442:Metabolome / Q000235:genetics; D055432:Metabolomics; D054647:Nutrigenomics
chemical_list: D015415:Biomarkers
keywords: NMR; ROC curve; biomarkers; choline metabolism; energy; legumes; metabolomics; pulses