title: The Expression Level of mRNA, Protein, and DNA Methylation Status ofFOSL2of Uyghur in XinJiang in Type 2 Diabetes.
abstract: Objective.We investigated the expression levels of bothFOSL2mRNA and protein as well as evaluating DNA methylation in the blood of type 2 diabetes mellitus (T2DM) Uyghur patients from Xinjiang. This study also evaluated whetherFOSL2gene expression had demonstrated any associations with clinical and biochemical indicators of T2DM.Methods.One hundred Uyghur subjects where divided into two groups, T2DM and nonimpaired glucose tolerance (NGT) groups. DNA methylation ofFOSL2was also analyzed byMassARRAYSpectrometry and methylation data of individual units were generated by the EpiTyper v1.0.5 software. The expression levels ofFOS-like antigen 2 (FOSL2)and the protein expression levels were analyzed.Results.Significant differences were observed in mRNA and protein levels when compared with the NGT group, while methylation rates of eight CpG units within theFOSL2gene were higher in the T2DM group. Methylation of CpG sites was found to inversely correlate with expression of other markers.Conclusions.Results show that a correlation between mRNA, protein, and DNA methylation ofFOSL2gene exists among T2DM patients from Uyghur.FOSL2protein and mRNA were downregulated and the DNA became hypermethylated, all of which may be involved in T2DM pathogenesis in this population.
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China.
authors.forename: Jun
authors.identifier: 0000-0002-1590-2336
authors.initials: J
authors.lastname: Li
authors.affiliation: Medical College, Shihezi University, Shihezi 832002, China.
authors.forename: Siyuan
authors.identifier: 0000-0003-0617-2279
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China.
authors.forename: Ying
authors.identifier: 0000-0003-2763-580X
authors.initials: Y
authors.lastname: Hu
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China; The First Department of General Medicine, The Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830000, China.
authors.forename: Guolei
authors.initials: G
authors.lastname: Cao
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China; Department of Endocrinology and Metabolism, The Fifth Affiliated Hospital, Xinjiang Medicine University, Urumqi, Xinjiang 830000, China.
authors.forename: Siyao
authors.identifier: 0000-0003-4362-1344
authors.initials: S
authors.lastname: Wang
authors.affiliation: Medical College, Shihezi University, Shihezi 832002, China.
authors.forename: Partab
authors.identifier: 0000-0002-6236-2408
authors.initials: P
authors.lastname: Rai
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China.
authors.forename: Xiaoli
authors.initials: X
authors.lastname: Wang
authors.affiliation: Department of Endocrinology and Metabolism, The First Affiliated Hospital, Shihezi University School of Medicine, Shihezi, Xinjiang 832002, China.
authors.forename: Kan
authors.identifier: 0000-0002-5007-5081
authors.initials: K
authors.lastname: Sun
mesh_terms: D000328:Adult; D000368:Aged; D002681:China; D019175:DNA Methylation; D003924:Diabetes Mellitus, Type 2 / Q000235:genetics / Q000378:metabolism*; D015536:Down-Regulation; D005260:Female; D006801:Humans; D008297:Male; D008875:Middle Aged; D016760:Proto-Oncogene Proteins c-fos / Q000235:genetics / Q000378:metabolism*; D012333:RNA, Messenger / Q000235:genetics / Q000378:metabolism; D000099169:Fos-Related Antigen 1
chemical_list: D016760:Proto-Oncogene Proteins c-fos; D012333:RNA, Messenger; D000099169:Fos-Related Antigen 1