title: Chitosan-ceramide coating on gold nanorod to improve its physiological stability and reduce the lipid surface-related toxicity.
abstract: Gold nanoparticles are promising materials for many applications that include imaging, drug delivery, and photothermal therapy. However, AuNPs can be unstable and/or toxic. We purposed to improve the stability and reduce toxicity of gold nanorods (AuNR) upon coating with biocompatible polymer, chitosan-ceramide (CS-CE), without replacing the original layer, CTAB. CS-CE-coated AuNR was prepared by simple mixing for 24 h and purified by centrifugation. The coating was confirmed by UV-Vis absorption analysis and surface charge and size measurement. We prepared nanorods with CS or CS-CE coating at two different concentrations (5 and 10% AuNR), the resulting in larger nanorods with a more positive surface-charge than that of AuNR. We investigated the UV-absorption and protein adsorption of the polymer coated nanorods. Based on the protein adsorption, AuNR-CS-CE was found to be more stable under physiological conditions than AuNR-CS. The cell internalization assay revealed that Hela cells internalized higher amounts of AuNR-CS-CE than that of AuNR-CS. Cytotoxicity study revealed that AuNR-CS-CE has lower toxicity than AuNR against HeLa cells. The CS-CE coating improved the stability of AuNR under physiological conditions via the hydrophobic interactions between the AuNR lipid surface and the ceramide anchor in the CS backbone as well as.
authors.affiliation: College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 406-799, South Korea.
authors.forename: Gantumur
authors.initials: G
authors.lastname: Battogtokh
authors.affiliation: College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 406-799, South Korea.
authors.forename: Oyuntuya
authors.initials: O
authors.lastname: Gotov
authors.affiliation: College of Pharmacy and Gachon Institute of Pharmaceutical Sciences, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 406-799, South Korea. youngtakko@gachon.ac.kr.
authors.forename: Young Tag
authors.initials: YT
authors.lastname: Ko
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry* / Q000633:toxicity*; D002470:Cell Survival; D002518:Ceramides / Q000737:chemistry*; D048271:Chitosan / Q000737:chemistry*; D003102:Colloids / Q000737:chemistry; D006046:Gold / Q000737:chemistry* / Q000633:toxicity*; D006367:HeLa Cells; D006801:Humans; D008055:Lipids / Q000737:chemistry* / Q000633:toxicity*; D043942:Nanotubes / Q000737:chemistry* / Q000633:toxicity*; D011485:Protein Binding; D013056:Spectrophotometry, Ultraviolet; D013499:Surface Properties
chemical_list: D001672:Biocompatible Materials; D002518:Ceramides; D003102:Colloids; D008055:Lipids; D006046:Gold; D048271:Chitosan
keywords: Ceramide; Chitosan; Colloidal stability; Gold nanorod; Polymer coating