title: microRNA expression changes after lipopolysaccharide treatment in gills of amphioxus Branchiostoma belcheri.
abstract: Recently, amphioxus has served as a model for studying the origin and evolution of vertebrate immunity. However, little is known about how microRNAs (miRNAs) are involved in the immune defense in amphioxus. In this article, we identified the amphioxus miRNAs in the acute-phase response to lipopolysaccharide (LPS). We determined the time point for the peak of immune response in amphioxus after LPS challenge by evaluating the expression of Branchiostoma belcheri toll-like receptor 1, NF-κb (c-Rel), and big defensin which react with pathogen-associated molecular patterns(PAMPs). Then we chose 12 h as the point to perform miRNA microarray analysis to select the differentially expressed miRNAs. Furthermore, we used quantitative real-time PCR to detect the expression patterns of selected amphioxus miRNAs under effective LPS challenge during the time course. The microarray data revealed that the miRNA expression file was significantly changed after LPS stimulation. The changes of the 10 most upregulated and 7 most downregualted miRNAs in gills of the amphioxus following challenge with LPS revealed a temporal induction kinetic. Our current study will provide valuable information to take an insight into molecular mechanism of innate immune and the evolution of the miRNA family.
authors.affiliation: School of Life Sciences, Nanjing University, China; Beihai Marine Station of Evo-devo Institute, Nanjing University, China.
authors.forename: Xin
authors.initials: X
authors.lastname: Liao
authors.affiliation: Department of Biomedical Research Center, The First People's Hospital of Kunming, China.
authors.forename: Liu
authors.initials: L
authors.lastname: Yang
authors.affiliation: School of Life Sciences, Nanjing University, China; Beihai Marine Station of Evo-devo Institute, Nanjing University, China.
authors.forename: Qilin
authors.initials: Q
authors.lastname: Zhang
authors.affiliation: Beihai Marine Station of Evo-devo Institute, Nanjing University, China; Nanjing Institute of Geology and Paleontology, Nanjing, China. Electronic address: junyuan_chen@126.com.
authors.forename: Junyuan
authors.initials: J
authors.lastname: Chen
mesh_terms: D000210:Acute-Phase Reaction / Q000276:immunology*; D000818:Animals; D005075:Biological Evolution; D002478:Cells, Cultured; D023082:Defensins / Q000235:genetics / Q000378:metabolism; D005880:Gills / Q000502:physiology*; D007113:Immunity, Innate; D064175:Lancelets / Q000276:immunology*; D008070:Lipopolysaccharides / Q000276:immunology; D035683:MicroRNAs / Q000235:genetics*; D046228:Microarray Analysis; D016328:NF-kappa B / Q000235:genetics / Q000378:metabolism; D000069452:Pathogen-Associated Molecular Pattern Molecules / Q000276:immunology; D051194:Toll-Like Receptor 1 / Q000235:genetics / Q000378:metabolism; D059467:Transcriptome
chemical_list: D023082:Defensins; D008070:Lipopolysaccharides; D035683:MicroRNAs; D016328:NF-kappa B; D000069452:Pathogen-Associated Molecular Pattern Molecules; D051194:Toll-Like Receptor 1
keywords: Branchiostoma belcheri; Innate immune; Lipopolysaccharide; microRNA