title: Toxicity Assessment of Binary Metal Mixtures (Copper-Zinc) to Nitrification in Soilless Culture with the Extended Biotic Ligand Model.
abstract: Metals are always found in the environment as mixtures rather than as solitary elements. Only a limited number of studies have developed appropriate models that incorporate bioavailability to estimate the toxicity of heavy-metal mixtures. In the present study, we explored the applicability of two extended biotic ligand model (BLM) approaches-BLM-fmixand BLM-toxicity unit (TU)-to predict and interpret mixture toxicity with the assumption that interactions between metal ions obey the BLM theory. Exposure assays of single and mixed metals were performed with inoculums of an ammonia-oxidizing bacterium SD5 isolated from soil. Nitrification of the cultures was the end point used to quantify the toxic response. The results indicated that the developed BLM-fmixapproach could well estimate the single toxicity of Cu2+and Zn2+as well as their binary mixture toxicity to nitrification with >90% of toxicity variation explained. Assuming that metal ions compete with each other for binding at a single biotic ligand, the BLM-fmixapproach (root-mean-square error [RMSE] = 19.66, R2= 0.8879) showed better predictive power than the BLM-TU approach (RMSE = 31.12, R2= 0.6892). The present study supports the use of the accumulation of metal ions at the biotic ligands as predictor of toxicity of single metals and metal mixtures.
authors.affiliation: Shandong University of Technology, Zibo, 255049, China.
authors.forename: Aiju
authors.initials: A
authors.lastname: Liu
authors.affiliation: Shandong University of Technology, Zibo, 255049, China.
authors.forename: JinXin
authors.initials: J
authors.lastname: Li
authors.affiliation: Shandong University of Technology, Zibo, 255049, China.
authors.forename: Menghong
authors.initials: M
authors.lastname: Li
authors.affiliation: Shandong University of Technology, Zibo, 255049, China.
authors.forename: Xiao Yin
authors.initials: XY
authors.lastname: Niu
authors.affiliation: Key Laboratory of Agriculture Environment in Universities of Shandong, College of Resources and Environment, Shandong Agricultural University, Taian, 271018, China. jwang@sdau.edu.cn.
authors.forename: Jun
authors.initials: J
authors.lastname: Wang
mesh_terms: D003300:Copper / Q000737:chemistry / Q000633:toxicity*; D004784:Environmental Monitoring / Q000379:methods*; D008024:Ligands; D008956:Models, Chemical; D058465:Nitrification; D012988:Soil Microbiology; D012989:Soil Pollutants / Q000737:chemistry / Q000633:toxicity*; D015032:Zinc / Q000737:chemistry / Q000633:toxicity*
chemical_list: D008024:Ligands; D012989:Soil Pollutants; D003300:Copper; D015032:Zinc