title: Effects of Extracts from Tiaozhi Granule and Its Components on Expression of Scavenger Receptor Class B Type I.
abstract: Sera from the rats with different drug treatments (atorvastatin, Tiaozhi granule, or its extracts) were collected. LO-2 cells or HepG2 cells were pretreated with different sera as the following groups randomly: (1) blank control group, (2) positive control group (atorvastatin group), (3) Tiaozhi granule water extract groups, (4) Tiaozhi granule alcohol extract groups, and (5) alcohol extracts for each component:Pollen Typhae Angustifoliae,Curcuma longaL., andRhizoma Alismatis. LO-2 cells were cotransfected with plasmid carrying SR-BI and pRL-TK promoter genes. Promoter activity was measured by the luciferase reporter gene assay. The mRNA and protein expressions of SR-BI were examined using real-time PCR and western blot analyses. Our results show that promoter activity and mRNA and protein expression levels of the SR-BI were significantly upregulated by Tiaozhi granules alcohol or water extracts in a dose-dependent manner.Pollen Typhae Angustifoliaealcohol extract with a high dosage could also increase SR-BI activity and expression, but not the extracts fromCurcuma longaL. andRhizoma Alismatis. Both Tiaozhi granule alcohol and water extracts can upregulate SR-BI gene expression. Among the components,Pollen Typhae Angustifoliaeare important for the regulatory effect coordinating withCurcuma longaL. andRhizoma Alismatis.
authors.affiliation: Laboratory of Cancer Molecular Genetics, Medical College of Soochow University, Dushu Lake Campus, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou 215123, China.
authors.forename: Xiao
authors.initials: X
authors.lastname: Yu
authors.affiliation: Department of Internal Medicine, The Affiliated Suzhou Chinese Traditional Medicine Hospital, Nanjing University of Chinese Medicine, 18 Yang-Su Road, Suzhou 215003, China.
authors.forename: Xiao-Dong
authors.initials: XD
authors.lastname: Zhao
authors.affiliation: Department of Internal Medicine, The Affiliated Suzhou Chinese Traditional Medicine Hospital, Nanjing University of Chinese Medicine, 18 Yang-Su Road, Suzhou 215003, China.
authors.forename: Rong-Qi
authors.initials: RQ
authors.lastname: Bao
authors.affiliation: Department of Internal Medicine, The Affiliated Suzhou Chinese Traditional Medicine Hospital, Nanjing University of Chinese Medicine, 18 Yang-Su Road, Suzhou 215003, China.
authors.forename: Jia-Yu
authors.initials: JY
authors.lastname: Yu
authors.affiliation: Department of Physiology, Medical College of Soochow University, Dushu Lake Campus, 199 Ren-Ai Road, Suzhou Industrial Park, Suzhou 215123, China.
authors.forename: Guo-Xing
authors.initials: GX
authors.lastname: Zhang
authors.affiliation: Department of Internal Medicine, The Affiliated Suzhou Chinese Traditional Medicine Hospital, Nanjing University of Chinese Medicine, 18 Yang-Su Road, Suzhou 215003, China.
authors.forename: Jing-Wei
authors.identifier: 0000-0001-5736-4807
authors.initials: JW
authors.lastname: Chen