title: Effect of Spinal Manometers on Cerebrospinal Fluid Amyloid-β Concentration.
abstract: The effect of spinal manometers on cerebrospinal fluid (CSF) amyloid-β (Aβ) concentration was investigated. Pooled human CSF samples were divided in two, one half passed through a manometer into a collection tube, the other transferred directly to a collection tube. CSF was analyzed for Aβ38/40/42 using an electrochemiluminescence immunoassay. Relative to control, use of a manometer decreased Aβ38/40/42 concentration by 5.6% (±1.5SE), 4.4% (±1.7SE), and 4.3% (±2.4SE), respectively. The ratios of Aβ42 :40, Aβ42 :38, and Aβ40 :38 were not affected by manometer treatment. Factors which artificially lower CSF Aβ concentrations are relevant to clinical diagnosis for AD and study design.
authors.affiliation: Department of Molecular Neuroscience, Institute of Neurology, University College London, London, UK.
authors.forename: Jamie
authors.initials: J
authors.lastname: Toombs
authors.affiliation: Department of Molecular Neuroscience, Institute of Neurology, University College London, London, UK.
authors.forename: Martha S
authors.initials: MS
authors.lastname: Foiani
authors.affiliation: Department of Neurodegeneration, Dementia Research Centre, Institute of Neurology, London, UK.
authors.forename: Ross W
authors.initials: RW
authors.lastname: Paterson
authors.affiliation: Department of Molecular Neuroscience, Institute of Neurology, University College London, London, UK.
authors.forename: Amanda
authors.initials: A
authors.lastname: Heslegrave
authors.affiliation: Department of Molecular Neuroscience, Institute of Neurology, University College London, London, UK.
authors.forename: Selina
authors.initials: S
authors.lastname: Wray
authors.affiliation: Department of Neurodegeneration, Dementia Research Centre, Institute of Neurology, London, UK.
authors.forename: Jonathan M
authors.initials: JM
authors.lastname: Schott
authors.affiliation: Department of Neurodegeneration, Dementia Research Centre, Institute of Neurology, London, UK.
authors.forename: Nick C
authors.initials: NC
authors.lastname: Fox
authors.affiliation: Department of Neuroimmunology, Institute of Neurology, University College London, London, UK.
authors.forename: Michael P
authors.initials: MP
authors.lastname: Lunn
authors.affiliation: Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.
authors.forename: Kaj
authors.initials: K
authors.lastname: Blennow
authors.affiliation: Department of Molecular Neuroscience, Institute of Neurology, University College London, London, UK.|Department of Psychiatry and Neurochemistry, Institute of Neuroscience and Physiology, The Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Zetterberg
mesh_terms: D016229:Amyloid beta-Peptides / Q000134:cerebrospinal fluid*; D015415:Biomarkers / Q000134:cerebrospinal fluid; D055664:Electrochemical Techniques; D006801:Humans; D008163:Luminescent Measurements; D008365:Manometry / Q000295:instrumentation; D013048:Specimen Handling; D013129:Spinal Puncture
chemical_list: D016229:Amyloid beta-Peptides; D015415:Biomarkers
keywords: Alzheimer’s disease; amyloid-β; biomarkers; cerebrospinal fluid; manometer; pre-analytical factor