title: Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo.
abstract: Salidriside (SDS), a phenylpropanoid glycoside derived from Rhodiola rosea L, has been shown to be neuroprotective in many studies, which may be promising in nerve recovery. In this study, the neuroprotective effects of SDS on engineered nerve constructed by Schwann cells (SCs) and Poly (lactic-co-glycolic acid) (PLGA) were studied in vitro. We further investigated the effect of combinational therapy of SDS and PLGA/SCs based tissue engineering on peripheral nerve regeneration based on the rat model of nerve injury by sciatic transection. The results showed that SDS dramatically enhanced the proliferation and function of SCs. The underlying mechanism may be that SDS affects SCs growth through the modulation of neurotrophic factors (BDNF, GDNF and CNTF). 12 weeks after implantation with a 12 mm gap of sciatic nerve injury, SDS-PLGA/SCs achieved satisfying outcomes of nerve regeneration, as evidenced by morphological and functional improvements upon therapy by SDS, PLGA/SCs or direct suture group assessed by sciatic function index, nerve conduction assay, HE staining and immunohistochemical analysis. Our results demonstrated the significant role of introducing SDS into neural tissue engineering to promote nerve regeneration.
authors.affiliation: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China.|The Collaborative Innovation Center of Guangxi Biological Medicine, Guangxi Medical University, Nanning, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Liu
authors.affiliation: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China.|Department of Spine Surgery, The Third Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Peizhen
authors.initials: P
authors.lastname: Lv
authors.affiliation: Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Yongjia
authors.initials: Y
authors.lastname: Zhu
authors.affiliation: Department of Cell Biology &Genetics, School of Premedical Sciences, Guangxi Medical University, Nanning, China.
authors.forename: Huayu
authors.initials: H
authors.lastname: Wu
authors.affiliation: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China.|Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.
authors.forename: Kun
authors.initials: K
authors.lastname: Zhang
authors.affiliation: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China.|The Medical and Scientific Research Center, Guangxi Medical University, Nanning, China.
authors.forename: Fuben
authors.initials: F
authors.lastname: Xu
authors.affiliation: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China.|The Collaborative Innovation Center of Guangxi Biological Medicine, Guangxi Medical University, Nanning, China.|The Medical and Scientific Research Center, Guangxi Medical University, Nanning, China.
authors.forename: Li
authors.initials: L
authors.lastname: Zheng
authors.affiliation: Guangxi Engineering Center in Biomedical Material for Tissue and Organ Regeneration, Guangxi Medical University, Nanning, China.|The Collaborative Innovation Center of Guangxi Biological Medicine, Guangxi Medical University, Nanning, China.|Department of Orthopaedics Trauma and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning, China.|Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Nanning, China.
authors.forename: Jinmin
authors.initials: J
authors.lastname: Zhao
mesh_terms: D000818:Animals; D002460:Cell Line; D002478:Cells, Cultured; D005960:Glucosides / Q000494:pharmacology*; D019344:Lactic Acid / Q000737:chemistry; D009414:Nerve Growth Factors / Q000378:metabolism; D009416:Nerve Regeneration; D018696:Neuroprotective Agents / Q000494:pharmacology*; D059348:Peripheral Nerve Injuries / Q000628:therapy*; D010636:Phenols / Q000494:pharmacology*; D011100:Polyglycolic Acid / Q000737:chemistry; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D051381:Rats; D017207:Rats, Sprague-Dawley; D012583:Schwann Cells / Q000187:drug effects* / Q000502:physiology; D012584:Sciatic Nerve / Q000187:drug effects* / Q000378:metabolism / Q000502:physiology; D023822:Tissue Engineering / Q000379:methods*; D054457:Tissue Scaffolds / Q000737:chemistry
chemical_list: D005960:Glucosides; D009414:Nerve Growth Factors; D018696:Neuroprotective Agents; D010636:Phenols; D000077182:Polylactic Acid-Polyglycolic Acid Copolymer; D011100:Polyglycolic Acid; D019344:Lactic Acid; C009172:rhodioloside