title: Epigallocatechin-3-gallate attenuates acrylamide-induced apoptosis and astrogliosis in rat cerebral cortex.
abstract: The potent neurotoxic agent acrylamide (ACR) is formed during Maillard reaction in food processing. Epigallocatechin-3-gallate (EGCG), a major bioactive component of green tea, is an antioxidant, but its effects on ACR-induced neurotoxicity are unclear. Here, we investigated the neuroprotective effects of EGCG against ACR-induced apoptosis and astrogliosis in the cerebral cortex. Rats were pretreated with EGCG for 4 d and then co-administered ACR for 14 d. Immunohistochemical analysis of glial fibrillary acidic protein and 8-hydroxy-2'-deoxyguanosine indicated that EGCG attenuated astrogliosis and DNA damage in ACR-treated rats. Analysis of DNA fragmentation and protein expression of Bax, Bcl-2, caspase 3, and cytochrome c revealed that EGCG inhibited ACR-induced apoptosis. Furthermore, EGCG inhibited oxidative stress by enhancing the activity of antioxidant enzymes and glutathione levels and reducing the formation of reactive oxygen species and lipid peroxidation. Taken together, our data demonstrate that EGCG inhibits ACR-induced apoptosis and astrogliosis in the cerebral cortex.
authors.affiliation: a College of Food , Shenyang Agricultural University , Shenhe District , Shenyang City , People's Republic of China.
authors.forename: Yin
authors.initials: Y
authors.lastname: He
authors.affiliation: a College of Food , Shenyang Agricultural University , Shenhe District , Shenyang City , People's Republic of China.
authors.forename: Dehong
authors.initials: D
authors.lastname: Tan
authors.affiliation: a College of Food , Shenyang Agricultural University , Shenhe District , Shenyang City , People's Republic of China.
authors.forename: Bing
authors.initials: B
authors.lastname: Bai
authors.affiliation: a College of Food , Shenyang Agricultural University , Shenhe District , Shenyang City , People's Republic of China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Wu
authors.affiliation: a College of Food , Shenyang Agricultural University , Shenhe District , Shenyang City , People's Republic of China.
authors.forename: Shujuan
authors.initials: S
authors.lastname: Ji
mesh_terms: D000080242:8-Hydroxy-2'-Deoxyguanosine; D020106:Acrylamide / Q000633:toxicity*; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001253:Astrocytes / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D002540:Cerebral Cortex / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D004249:DNA Damage; D003849:Deoxyguanosine / Q000031:analogs & derivatives / Q000378:metabolism; D004305:Dose-Response Relationship, Drug; D005904:Glial Fibrillary Acidic Protein / Q000378:metabolism; D005911:Gliosis / Q000517:prevention & control*; D015227:Lipid Peroxidation / Q000187:drug effects; D008297:Male; D018696:Neuroprotective Agents / Q000494:pharmacology*; D018384:Oxidative Stress / Q000187:drug effects; D017207:Rats, Sprague-Dawley
chemical_list: D005904:Glial Fibrillary Acidic Protein; D018696:Neuroprotective Agents; D020106:Acrylamide; D000080242:8-Hydroxy-2'-Deoxyguanosine; D002392:Catechin; C045651:epigallocatechin gallate; D003849:Deoxyguanosine
keywords: Acrylamide; apoptosis; astrogliosis; epigallocatechin-3-gallate; oxidative stress