title: Synthesis of a Novel IR-822-Met near-infrared probe for in vivo tumor diagnosis.
abstract: OBJECTIVE
  Methionine is a valid target for the treatment of cancer and to achieve in vivo imaging and early diagnosis of tumors, we have synthesized near-infrared (NIR) fluorochrome IR-822-labeled methionine (IR-822-Met).
  
  
  RESULTS
  NIR fluorescent dye IR-822 was conjugated with methionine through its amide bond. It had low toxicity to normal cell/tissues. In vitro and in vivo studies demonstrated its high targeting capability to tumors. The results support the potential of using ligand-modified methionine probe for tumor diagnosis and targeted therapy. The probe also exhibited good photostability, and excellent cell membrane permeability.
  
  
  CONCLUSIONS
  IR-822-Met is a promising imaging agent for tumor diagnosis, especially in their early stage.
authors.affiliation: School of Life Sciences, Binzhou University, Binzhou, 256600, China.
authors.forename: Chunlong
authors.initials: C
authors.lastname: Sun
authors.affiliation: Qingdao Municipal Hospital (Group), Qingdao, 266011, China.
authors.forename: Hongtao
authors.initials: H
authors.lastname: Zhang
authors.affiliation: School of Life Sciences, Binzhou University, Binzhou, 256600, China.
authors.forename: Wen
authors.initials: W
authors.lastname: Du
authors.affiliation: School of Life Sciences, Binzhou University, Binzhou, 256600, China.
authors.forename: Baoqin
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Biological Science and Medical Engineering & Collaborative Innovation Center of Suzhou NanoScience and Technology, Southeast University, Suzhou, 215123, China. seujimin@sina.cn.|School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China. seujimin@sina.cn.
authors.forename: Min
authors.initials: M
authors.lastname: Ji
mesh_terms: D000818:Animals; D002232:Carbocyanines / Q000138:chemical synthesis*; D045744:Cell Line, Tumor; D005456:Fluorescent Dyes / Q000138:chemical synthesis*; D006801:Humans; D061986:MCF-7 Cells; D008297:Male; D008715:Methionine / Q000138:chemical synthesis*; D051379:Mice; D008819:Mice, Nude; D009369:Neoplasms / Q000000981:diagnostic imaging*; D061848:Optical Imaging; D019265:Spectroscopy, Near-Infrared
chemical_list: D002232:Carbocyanines; D005456:Fluorescent Dyes; C000617014:IR-822-Met; D008715:Methionine
keywords: Fluorescent dye; IR-822-Met; L-Methionine; Near-infrared fluorescence imaging; Tumor diagnosis