title: Differential expression and miRNA regulation of the GSTP1 gene in the regenerating liver of Chiloscyllium plagiosum.
abstract: Liver regeneration is a complicated process, and understanding the regulatory mechanism will be helpful in the treatment of diseases associated with liver. In this study, the one-third liver resection model was established in Chiloscyllium plagiosum, and the whole transcriptome of the C. plagiosum was generated using the Illumina-Solexa sequencing platform. Differentially expressed genes were analyzed using bioinformatics methods and verified using quantitative real-time PCR (qRT-PCR). Using miRanda and TargetScan, we screened the microRNA library for miRNAs that target the glutathione S-transferase P1(GSTP1) gene. Dual-luciferase reporter assays were used to confirm binding between the miRNA and GSTP1. Finally, we used western blotting analysis to determine expression of the GSTP1 protein. As a result, 65,356 unigenes were obtained in normal and damaged liver tissues, with mean length of 955 bp. A total of 359 differentially expressed genes were acquired; 217 of which were upregulated, and 142 were downregulated, including the GSTP1 gene, following liver resection. The presence of the GSTP1 protein in C. plagiosum was shown for the first time. Luciferase reporter assay revealed that GSTP1 messenger RNA was targeted by ipu-miR-143. The discovery and differential expression analysis of GSTP1 in C. plagiosum will be a valuable resource to explain the molecular mechanism of GSTP1 regulation of liver repair.
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Yinghua
authors.initials: Y
authors.lastname: Ge
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Jiewen
authors.initials: J
authors.lastname: Zhang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Xinyi
authors.initials: X
authors.lastname: Shi
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Conger
authors.initials: C
authors.lastname: Lu
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Lingrong
authors.initials: L
authors.lastname: Yang
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Yuanyuan
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Yanna
authors.initials: Y
authors.lastname: Chen
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Dandan
authors.initials: D
authors.lastname: Cheng
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Bai
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China. zhengbingl@zstu.edu.cn.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China. zhengbingl@zstu.edu.cn.
authors.forename: Zhengbing
authors.initials: Z
authors.lastname: Lv
authors.affiliation: College of Life Sciences, Zhejiang Sci-Tech University, Hangzhou, 310018, China. llliu@zstu.edu.cn.|Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou, 310018, China. llliu@zstu.edu.cn.
authors.forename: Lili
authors.initials: L
authors.lastname: Liu
mesh_terms: D020413:3' Untranslated Regions; D000818:Animals; D004247:DNA / Q000235:genetics; D015971:Gene Expression Regulation, Enzymologic / Q000502:physiology*; D005982:Glutathione Transferase / Q000235:genetics / Q000378:metabolism*; D008099:Liver / Q000378:metabolism*; D008115:Liver Regeneration / Q000502:physiology*; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D060888:Real-Time Polymerase Chain Reaction; D012754:Sharks / Q000502:physiology*; D059467:Transcriptome
chemical_list: D020413:3' Untranslated Regions; D035683:MicroRNAs; D004247:DNA; D005982:Glutathione Transferase
keywords: Chiloscyllium plagiosum; Glutathione S-transferase P1; Liver regeneration; MicroRNA; Transcriptome