title: Malonyl-CoA pathway: a promising route for 3-hydroxypropionate biosynthesis.
abstract: 3-Hydroxypropionate (3HP) is an attractive platform chemical, serving as a precursor to a variety of commodity chemicals like acrylate and acrylamide, as well as a monomer of a biodegradable plastic. To establish a sustainable way to produce these commercially important chemicals and materials, fermentative production of 3HP is widely investigated in recent years. It is reported that 3HP can be produced from several intermediates, such as glycerol, malonyl-CoA, and β-alanine. Among all these biosynthetic routes, the malonyl-CoA pathway has some distinct advantages, including a broad feedstock spectrum, thermodynamic feasibility, and redox neutrality. To date, this pathway has been successfully constructed in various species including Escherichia coli, yeast and cyanobacteria, and optimized through carbon flux redirection, enzyme screening and engineering, and an increasing supply of energy and cofactors, resulting in significantly enhanced 3HP titer up to 40 g/L. These results show the feasibility of commercial manufacturing of 3HP and its derivatives in the future.
authors.affiliation: a CAS Key Lab of Biobased Materials , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.|b Institute of Oceanology , Chinese Academy of Sciences , Qingdao , China.
authors.forename: Changshui
authors.initials: C
authors.lastname: Liu
authors.affiliation: b Institute of Oceanology , Chinese Academy of Sciences , Qingdao , China.
authors.forename: Yamei
authors.initials: Y
authors.lastname: Ding
authors.affiliation: a CAS Key Lab of Biobased Materials , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Mo
authors.initials: M
authors.lastname: Xian
authors.affiliation: a CAS Key Lab of Biobased Materials , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: a CAS Key Lab of Biobased Materials , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Huizhou
authors.initials: H
authors.lastname: Liu
authors.affiliation: b Institute of Oceanology , Chinese Academy of Sciences , Qingdao , China.
authors.forename: Qingjun
authors.initials: Q
authors.lastname: Ma
authors.affiliation: a CAS Key Lab of Biobased Materials , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , Qingdao , China.
authors.forename: Guang
authors.identifier: http://orcid.org/0000-0003-0002-5972
authors.initials: G
authors.lastname: Zhao
mesh_terms: D004926:Escherichia coli; D019344:Lactic Acid; D008316:Malonyl Coenzyme A / Q000378:metabolism*; D010084:Oxidation-Reduction; D012441:Saccharomyces cerevisiae
chemical_list: D019344:Lactic Acid; D008316:Malonyl Coenzyme A
keywords: 3-Hydroxypropionate; 3HP tolerance; acetyl-CoA carboxylase; carbon flux; enzyme engineering; malonyl-CoA pathway; malonyl-CoA reductase; metabolic engineering; supply of energy and cofactor