title: Evaluation of P2X7 receptor expression in peripheral lymphocytes and immune profile from patients with indeterminate form of Chagas disease.
abstract: Chagas disease (CD) is caused by Trypanosoma cruzi, an intracellular protozoan which is a potent stimulator of cell-mediated immunity. In the indeterminate form of CD (IFCD) a modulation between pro- and anti-inflammatory responses establishes a host-parasite adaptation. It was previously demonstrated that purinergic ecto-enzymes regulates extracellular ATP and adenosine levels, influencing immune and inflammatory processes during IFCD. In inflammatory sites ATP, as well as its degradation product, adenosine, function as signaling molecules and immunoregulators through the activation of purinergic receptors. In this work, it was analyzed the gene and protein expression of P2X7 purinergic receptor in peripheral lymphocytes and serum immunoregulatory cytokines from IFCD patients. Gene and protein expression of P2X7 receptor (P2X7R), and serum cytokines (IL-2, IL-10, IL-17 and IFN-γ) were unaltered. However, IFCD group showed significantly higher IL-4 and IL-6 levels while TNF-α was significantly decreased. These results indicate that imune profile of IFCD patients displays anti-inflammatory characteristics, consistent with the establishment of an immunomodulatory response. Further study about the molecular knowledge of P2X7R in IFCD is useful to clarify the participation of purinergic system in the regulatory mechanism which avoid the progression of CD.
authors.affiliation: Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Viviane do Carmo Gonçalves
authors.initials: VD
authors.lastname: Souza
authors.affiliation: Department of Large Animal Clinic de Clínica, Rural Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Joabel Tonellotto
authors.initials: JT
authors.lastname: Dos Santos
authors.affiliation: Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Fernanda Licker
authors.initials: FL
authors.lastname: Cabral
authors.affiliation: Department of Morphology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Fernanda
authors.initials: F
authors.lastname: Barbisan
authors.affiliation: Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Maria Isabel
authors.initials: MI
authors.lastname: Azevedo
authors.affiliation: Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Luiz Felipe
authors.initials: LF
authors.lastname: Dias Carli
authors.affiliation: Department of Preventive Veterinary Medicine, Rural Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil.
authors.forename: Sonia
authors.initials: S
authors.lastname: de Avila Botton
authors.affiliation: Center of Biological and Health Sciences, Federal University of Mato Grosso do Sul, Campo Grande, MS 79.070-900, Brazil.
authors.forename: Jeandre Augusto
authors.initials: JA
authors.lastname: Dos Santos Jaques
authors.affiliation: Department of Microbiology and Parasitology, Health Sciences Center, Federal University of Santa Maria, Santa Maria, RS 97105-900, Brazil. Electronic address: dbitencourtrosaleal@gmail.com.
authors.forename: Daniela Bitencourt
authors.initials: DB
authors.lastname: Rosa Leal
mesh_terms: D016022:Case-Control Studies; D014355:Chagas Disease / Q000175:diagnosis / Q000235:genetics* / Q000276:immunology* / Q000469:parasitology; D016207:Cytokines / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D007111:Immunity, Cellular; D056747:Immunomodulation; D018836:Inflammation Mediators / Q000378:metabolism; D008214:Lymphocytes / Q000276:immunology* / Q000378:metabolism*; D008297:Male; D008875:Middle Aged; D058486:Receptors, Purinergic P2X7 / Q000235:genetics* / Q000378:metabolism
chemical_list: D016207:Cytokines; D018836:Inflammation Mediators; D058486:Receptors, Purinergic P2X7
keywords: Chagas disease; Cytokines; Lymphocytes; P2X7