title: Oxazolone-Induced Intestinal Inflammation in Adult Zebrafish.
abstract: Zebrafish are an excellent model for the study of intestinal immunity. The availability of several transgenic reporter fish for different innate and adaptive immune cells and the high homology in terms of gut function and morphology enables in depth analysis of the process of intestinal inflammation. Here, we describe a method to induce intestinal inflammation by intra-rectal injection of the hapten oxazolone in adult zebrafish.
authors.affiliation: Animal Sciences Group, Cell Biology and Immunology, Wageningen University, De Elst 1, 6708, WD, Wageningen, The Netherlands. Sylvia.brugman@wur.nl.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Brugman
authors.affiliation: Department of Pediatrics, Wilhelmina Children's Hospital Utrecht, University Medical Center Utrecht, Utrecht, The Netherlands.
authors.forename: Edward E S
authors.initials: EES
authors.lastname: Nieuwenhuis
mesh_terms: D000285:Administration, Rectal; D062365:Aminobenzoates; D018681:Anesthetics, General; D000818:Animals; D030801:Animals, Genetically Modified; D003424:Crohn Disease / Q000139:chemically induced / Q000276:immunology / Q000473:pathology*; D004195:Disease Models, Animal; D005260:Female; D015870:Gene Expression; D017930:Genes, Reporter; D049452:Green Fluorescent Proteins / Q000235:genetics / Q000378:metabolism; D006651:Histocytochemistry / Q000379:methods*; D006801:Humans; D018928:Immunity, Mucosal; D007413:Intestinal Mucosa / Q000276:immunology / Q000473:pathology*; D008164:Luminescent Proteins / Q000235:genetics / Q000378:metabolism; D008297:Male; D010081:Oxazolone; D012720:Severity of Illness Index; D015027:Zebrafish / Q000276:immunology; D000097573:Red Fluorescent Protein
chemical_list: D062365:Aminobenzoates; D018681:Anesthetics, General; D049452:Green Fluorescent Proteins; D008164:Luminescent Proteins; D010081:Oxazolone; D000097573:Red Fluorescent Protein; C003636:tricaine
keywords: Crohn’s disease; Granulocytes; Gut barrier function; IBD; Intestinal inflammation; Intra-rectal injection; Oxazolone; Ulcerative colitis; Zebrafish