title: Live births after polar body biopsy and frozen-thawed cleavage stage embryo transfer: case report.
abstract: Pre-implantation genetic diagnosis (PGD) or screening (PGS) technology, has emerged and developed in the past few years, benefiting couples as it allows the selection and transfer of healthy embryos during IVF treatments. These techniques can be performed in oocytes (polar-body biopsy) or embryos (blastomere or trophectoderm biopsy). In this case report, we describe the first two live births to be published in Brazil after a polar-body (PB) biopsy. In case 1, a 42-year-old was submitted to PB biopsy with PGS due to advanced maternal age and poor ovarian reserve. Five MII oocytes underwent first and second polar body biopsy and four cleavage embryos were cryopreserved. The PGS analysis resulted in two euploid embryos (next generation sequence). A frozen-thawed embryo transfer (FET) was performed after endometrial priming and a healthy baby was delivered after a cesarean section (37 weeks, female, 3390g, 47.5 cm). In case 2, a 40-year old patient with balanced translocation and poor ovarian response was submitted to PB biopsy. Two MII oocytes underwent first and second polar body biopsy and two embryos were cryopreserved in cleavage stage. The analysis resulted in one euploid embryo that was transferred after endometrial priming. A preterm healthy baby (34 weeks, female, 2100g, 40 cm) was delivered via cesarean section. In conclusion, although the blastocyst biopsy is the norm when performing PGS/PGD during IVF treatments, other alternatives (as PB biopsy) should be considered in some specific situations.
authors.affiliation: ORIGEN - Center for Reproductive Medicine, Rio de Janeiro/RJ - Brazil.
authors.forename: Fernando
authors.initials: F
authors.lastname: Guimarães
authors.affiliation: ORIGEN - Center for Reproductive Medicine, Rio de Janeiro/RJ - Brazil.|UFMG - Universidade Federal de Minas Gerais, Belo Horizonte/MG - Brazil.
authors.forename: Matheus
authors.initials: M
authors.lastname: Roque
authors.affiliation: ORIGEN - Center for Reproductive Medicine, Rio de Janeiro/RJ - Brazil.
authors.forename: Marcello
authors.initials: M
authors.lastname: Valle
authors.affiliation: ORIGEN - Center for Reproductive Medicine, Rio de Janeiro/RJ - Brazil.
authors.forename: Alessandra
authors.initials: A
authors.lastname: Kostolias
authors.affiliation: ORIGEN - Center for Reproductive Medicine, Rio de Janeiro/RJ - Brazil.
authors.forename: Rodrigo A de
authors.initials: RA
authors.lastname: Azevedo
authors.affiliation: Chromosome Genomic Medicine, São Paulo/SP - Brazil.
authors.forename: Ciro D
authors.initials: CD
authors.lastname: Martinhago
authors.affiliation: ORIGEN - Center for Reproductive Medicine, Belo Horizonte/MG - Brazil.
authors.forename: Marcos
authors.initials: M
authors.lastname: Sampaio
authors.affiliation: UFMG - Universidade Federal de Minas Gerais, Belo Horizonte/MG - Brazil.|ORIGEN - Center for Reproductive Medicine, Belo Horizonte/MG - Brazil.
authors.forename: Selmo
authors.initials: S
authors.lastname: Geber
keywords: PGD; PGS; Polar body biopsy; pre-implantation genetic diagnosis; pre-implantation genetic screening