title: Adenovirus transduction: More complicated than receptor expression.
abstract: The abundance and accessibility of a primary virus receptor are critical factors that impact the susceptibility of a host cell to virus infection. The Coxsackievirus and adenovirus receptor (CAR) has two transmembrane isoforms that occur due to alternative splicing and differ in localization and function in polarized epithelia. To determine the relevance of isoform-specific expression across cell types, the abundance and localization of both isoforms were determined in ten common cell lines, and correlated with susceptibility to adenovirus transduction relative to polarized primary human airway epithelia. Data show that the gene and protein expression for each isoform of CAR varies significantly between cell lines and polarization, as indicated by high transepithelial resistance, is inversely related to adenovirus transduction. In summary, the variability of polarity and isoform-specific expression among model cells are critical parameters that must be considered when evaluating the clinical relevance of potential adenovirus-mediated gene therapy and anti-adenovirus strategies.
authors.affiliation: Department of Biological Sciences, Wright State University, Dayton, OH, USA.
authors.forename: Priyanka
authors.initials: P
authors.lastname: Sharma
authors.affiliation: Biomedical Sciences PhD Program, Wright State University, Dayton, OH 45435, USA.
authors.forename: Prithy C
authors.initials: PC
authors.lastname: Martis
authors.affiliation: Department of Biological Sciences, Wright State University, Dayton, OH, USA; Biomedical Sciences PhD Program, Wright State University, Dayton, OH 45435, USA. Electronic address: katherine.excoffon@wright.edu.
authors.forename: Katherine J D A
authors.initials: KJDA
authors.lastname: Excoffon
mesh_terms: D000256:Adenoviridae / Q000235:genetics* / Q000502:physiology; D000257:Adenoviridae Infections / Q000235:genetics / Q000378:metabolism* / Q000503:physiopathology / Q000821:virology; D002460:Cell Line; D016764:Cell Polarity; D002478:Cells, Cultured; D004847:Epithelial Cells / Q000166:cytology / Q000378:metabolism / Q000821:virology*; D006801:Humans; D020033:Protein Isoforms / Q000235:genetics / Q000378:metabolism; D011991:Receptors, Virus / Q000235:genetics / Q000378:metabolism*; D012137:Respiratory System / Q000166:cytology / Q000378:metabolism / Q000821:virology; D014161:Transduction, Genetic
chemical_list: D020033:Protein Isoforms; D011991:Receptors, Virus
keywords: Adenovirus; Apical and basolateral; Coxsackievirus and adenovirus receptor (CAR); Epithelia; Non-polarized; Polarized; Tight junction