title: Interplay Between Long Noncoding RNA ZEB1-AS1 and miR-200s Regulates Osteosarcoma Cell Proliferation and Migration.
abstract: In our previous study, we found long noncoding RNA ZEB1-AS1 is upregulated and functions as an oncogene in osteosarcoma. MiR-200 family (miR-200s) functions as tumor suppressor via directly targeting ZEB1 in various cancers. In this study, we further investigate the potential interplay between ZEB1-AS1, miR-200s, and ZEB1 in osteosarcoma. Our results showed that ZEB1-AS1 functions as a molecular sponge for miR-200s and relieves the inhibition of ZEB1 caused by miR-200s. ZEB1-AS1 and miR-200s reciprocally negatively regulate each other. MiR-200s are downregulated in osteosarcoma tissues, and negatively correlated with ZEB1-AS1 and ZEB1 expression levels in osteosarcoma. Functional experiments showed that consistent with ZEB1-AS1 depletion, miR-200s overexpression and ZEB1 depletion both inhibit osteosarcoma cell proliferation and migration. Overexpression of miR-200s partially abolished the effects of ZEB1-AS1 on osteosarcoma cell proliferation and migration. Moreover, the combination of ZEB1-AS1 depletion and miR-200s overexpression significantly inhibits osteosarcoma cell proliferation and migration. In conclusion, this study revealed a novel regulatory mechanism between ZEB1-AS1, miR-200s, and ZEB1. The interplay between ZEB1-AS1 and miR-200s contributes to osteosarcoma cell proliferation and migration, and targeting this interplay could be a promising strategy for osteosarcoma treatment. J. Cell. Biochem. 118: 2250-2260, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: Department of Clinical Laboratory, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Chibo
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Clinical Laboratory, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Chunqin
authors.initials: C
authors.lastname: Pan
authors.affiliation: Department of Clinical Laboratory, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Yanqun
authors.initials: Y
authors.lastname: Cai
authors.affiliation: Department of Orthopedics, Taizhou Municipal Hospital, Taizhou, Zhejiang, China.
authors.forename: Haibao
authors.initials: H
authors.lastname: Wang
mesh_terms: D015152:Blotting, Northern; D015153:Blotting, Western; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics / Q000502:physiology; D049109:Cell Proliferation / Q000235:genetics / Q000502:physiology; D015972:Gene Expression Regulation, Neoplastic / Q000235:genetics / Q000502:physiology; D006801:Humans; D047468:Immunoprecipitation; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D012516:Osteosarcoma / Q000235:genetics / Q000378:metabolism*; D016133:Polymerase Chain Reaction; D062085:RNA, Long Noncoding / Q000235:genetics / Q000378:metabolism*
chemical_list: C569392:MIRN202 microRNA, human; D035683:MicroRNAs; D062085:RNA, Long Noncoding; C000610978:long non-coding RNA ZEB1-AS1, human
keywords: CELL MIGRATION; CELL PROLIFERATION; LONG NONCODING RNA; OSTEOSARCOMA; ZEB1-AS1; miR-200 FAMILY; microRNA