title: Association ofCOX-2Promoter Polymorphisms -765G/C and -1195A/G with Migraine.
abstract: BACKGROUND
  Migraine is a common debilitating primary headache disorder with current head pain attacks, which contributes to physical activity dysfunctions in chronic pain phase. PGE2 and PGI2 are two important prostaglandins synthesised byCOX-2enzymes, involved in migraine pain signals.COX-2modulation is essential in treatment and pathogenesis of migraine. This study aimed to investigating the association betweenCOX-2gene polymorphisms with the risk of migraine susceptibility in migraine patients with related and unrelated parents.
  
  
  METHODS
  This case- control study was based on 100 migraine patients and 100 non-migraine subjects in Bushehr province, Iran in 2013. Genomic DNA of blood samples was extracted and genotyping ofCOX-2-765G>C (rs20417)andCOX-2-1195A>G (rs689466)gene variants was investigated by PCR-RFLP method. Statistical analyses were accomplished using the SPSS software package.
  
  
  RESULTS
  There was a significant differences in the frequencies of theCOX-2-765G>CandCOX-2-1195A>Ggenotypes between migraine patients and controls (P≤0.05).
  
  
  CONCLUSIONS
  COX-2-765CC,COX-2-765CG,COX-2-1195GGandCOX-2-1195AGgenotypes can increase the risk of migraine significantly. As the first study in Iran, we are hopeful to achieve greater results about the relevancy ofCOX-2gene, migraine and pain signals pathway by repeating these experiments on more samples.
authors.affiliation: Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
authors.forename: Elahe
authors.initials: E
authors.lastname: Mozaffari
authors.affiliation: Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran.
authors.forename: Abbas
authors.initials: A
authors.lastname: Doosti
authors.affiliation: Young Researchers and Elite Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
authors.forename: Asghar
authors.initials: A
authors.lastname: Arshi
authors.affiliation: Dept. of Animal Sciences, Faculty of Agriculture, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran.
authors.forename: Mostafa
authors.initials: M
authors.lastname: Faghani
keywords: COX-2 gene; Iran; Migraine; Polymorphism; RFLP