title: Distribution of rhodopin and spirilloxanthin between LH1 and LH2 complexes when incorporating carotenoid mixture into the membrane of purple sulfur bacterium Allochromatium minutissimum in vitro.
abstract: Carotenoid mixture enriched by rhodopin and spirilloxanthin was incorporated in LH2 and LH1 complexes from Allochromatium (Alc.) minutissimum in vitro. The maximum incorporating level was ~95%. Rhodopin (56.4%) and spirilloxanthin (13.8%) were incorporated into the LH1 complex, in contrast to the control complex, which contained primarily spirilloxanthin (66.8%). After incorporating, the LH2 complex contained rhodopin (66.7%) and didehydrorhodopin (14.6%), which was close to their content in the control (67.4 and 20.5%, respectively). Thus, it was shown that carotenoids from the total pool are not selectively incorporated into LH2 and LH1 complexes in vitro in the proportion corresponding to the carotenoid content in the complexes in vivo.
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia. lfbv22@gmail.com.
authors.forename: M A
authors.initials: MA
authors.lastname: Bolshakov
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Ashikhmin
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: Z K
authors.initials: ZK
authors.lastname: Makhneva
authors.affiliation: Institute of Basic Biological Problems, Russian Academy of Sciences, ul. Institutskaya 2, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: A A
authors.initials: AA
authors.lastname: Moskalenko
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D002338:Carotenoids / Q000378:metabolism*; D002462:Cell Membrane / Q000378:metabolism*; D002841:Chromatiaceae / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D045342:Light-Harvesting Protein Complexes / Q000378:metabolism*; D013057:Spectrum Analysis; D024341:Xanthophylls / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D045342:Light-Harvesting Protein Complexes; D024341:Xanthophylls; D002338:Carotenoids; C113102:rhodopin; C055255:spirilloxanthin