title: Corilagin ameliorates the extreme inflammatory status in sepsis through TLR4 signaling pathways.
abstract: BACKGROUND
  Sepsis is one of the serious disorders in clinical practice. Recent studies found toll-like receptors 4 (TLR4) played an important role in sepsis. In this study, we tried to find the influence of Corilagin on TLR4 signal pathways in vitro and in vivo.
  
  
  METHODS
  The cellular and animal models of sepsis were established by LPS and then interfered with Corilagin. Real-time PCR and western blot were employed to detect the mRNA and protein expressions of TLR4, MyD88, TRIF and TRAF6. ELISA was used to determine the IL-6 and IL-1β levels in supernatant and serum.
  
  
  RESULTS
  The survival rate was improved in the LPS + Corilagin group, and the mRNA and protein expressions of TLR4, MyD88, TRIF and TRAF6 were significantly decreased than that in the LPS group both in cellular and animal models (P < 0.01). The pro-inflammatory cytokines IL-6 and IL-1β were greatly decreased in the LPS + Corilagin group both in supernatant and serum (P < 0.01).
  
  
  CONCLUSIONS
  Corilagin exerts the anti-inflammatory effects by down-regulating the TLR4 signaling molecules to ameliorate the extreme inflammatory status in sepsis.
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.|Department of Integrated Traditional Chinese and Western Medicine, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
authors.forename: Hua-Rong
authors.initials: HR
authors.lastname: Li
authors.affiliation: Department of Intensive Care Unit, Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, People's Republic of China.
authors.forename: Jie
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
authors.forename: Shu-Ling
authors.initials: SL
authors.lastname: Zhang
authors.affiliation: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
authors.forename: Tao
authors.initials: T
authors.lastname: Luo
authors.affiliation: Department of Neurology, Wuhan General Hospital of Guangzhou Military Command, Wuhan, 430070, People's Republic of China.
authors.forename: Fei
authors.initials: F
authors.lastname: Wu
authors.affiliation: Central Lab, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
authors.forename: Ji-Hua
authors.initials: JH
authors.lastname: Dong
authors.affiliation: Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China.
authors.forename: Yuan-Jin
authors.initials: YJ
authors.lastname: Guo
authors.affiliation: Department of Infectious Diseases, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, 430022, People's Republic of China. leizhao@hust.edu.cn.
authors.forename: Lei
authors.initials: L
authors.lastname: Zhao
mesh_terms: D000818:Animals; D005960:Glucosides / Q000008:administration & dosage*; D006801:Humans; D047348:Hydrolyzable Tannins / Q000008:administration & dosage*; D053583:Interleukin-1beta / Q000235:genetics / Q000276:immunology; D015850:Interleukin-6 / Q000235:genetics / Q000276:immunology; D008297:Male; D051379:Mice; D008807:Mice, Inbred BALB C; D053594:Myeloid Differentiation Factor 88 / Q000235:genetics / Q000276:immunology; D000067996:RAW 264.7 Cells; D018805:Sepsis / Q000188:drug therapy* / Q000235:genetics / Q000276:immunology; D015398:Signal Transduction / Q000187:drug effects; D051197:Toll-Like Receptor 4 / Q000235:genetics / Q000276:immunology*
chemical_list: D005960:Glucosides; D047348:Hydrolyzable Tannins; D053583:Interleukin-1beta; D015850:Interleukin-6; D053594:Myeloid Differentiation Factor 88; C493487:Tlr4 protein, mouse; D051197:Toll-Like Receptor 4; C049096:corilagin
keywords: Corilagin; Epsis; Pro-inflammatory cytokines; Signal pathway; TLR4