title: Establishment and Utilization of Habituated Cell Suspension Cultures for Hormone-Inducible Xylogenesis.
abstract: The development of inducible cell differentiation in suspension cultures led to multiple breakthroughs. It enabled the understanding of the chronology, duration, regulation and interdependency of the multiple events leading to fully functional specialized cells. The most studied cell differentiation in plants using inducible suspension cultures is the formation of tracheary elements (TEs) - the hydro-mineral sap conducting cells. Several in vitro systems established in different plant species have been developed to trigger TE formation on-demand. Here, we describe the establishment, harvesting and analysis of Arabidopsis thaliana stable habituated cell lines inducible by hormones to differentiate into TEs on-demand. Moreover, we explain the means to monitor and modify the chronology, duration and regulation of the progression of TE formation.
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.|Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91, Stockholm, Sweden.
authors.forename: Delphine
authors.initials: D
authors.lastname: Ménard
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.|Department of Medical Biochemistry and Biophysics, Umeå University, 901 87, Umeå, Sweden.
authors.forename: Henrik
authors.initials: H
authors.lastname: Serk
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden.
authors.forename: Raphaël
authors.initials: R
authors.lastname: Decou
authors.affiliation: Umeå Plant Science Centre (UPSC), Department of Plant Physiology, Umeå University, 901 87, Umeå, Sweden. edouard.pesquet@su.se.|Arrhenius Laboratories, Department of Ecology, Environment and Plant Sciences (DEEP), Svante Arrhenius väg 20A, Stockholm University, 160 91, Stockholm, Sweden. edouard.pesquet@su.se.
authors.forename: Edouard
authors.initials: E
authors.lastname: Pesquet
mesh_terms: D017360:Arabidopsis / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*; D018929:Cell Culture Techniques; D002454:Cell Differentiation / Q000187:drug effects; D008856:Microscopy, Fluorescence; D052585:Phloem / Q000166:cytology / Q000187:drug effects / Q000254:growth & development; D010937:Plant Growth Regulators / Q000494:pharmacology*; D014170:Transformation, Genetic; D052584:Xylem / Q000166:cytology / Q000187:drug effects* / Q000254:growth & development*
chemical_list: D010937:Plant Growth Regulators
keywords: Arabidopsis thaliana; Habituated cell cultures; Hormone inducible differentiation; Tracheary elements