title: Study on the mitochondrial apoptosis pathways of small cell lung cancer H446 cells induced by Trichinella spiralis muscle larvae ESPs.
abstract: Trichinella spiralis (T.spiralis) muscle-larva (ML) excretory-secretory proteins (ESPs) contain antitumour-active substances. ESPs have been shown to inhibit tumour growth. To explore the effects of these proteins on small cell lung cancer cells and the possible mechanisms of their antineoplastic action, H446 SCLC cells were co-cultured with different concentrations of T. spiralis ML ESPs for 12, 24 and 48 h. Our results showed that T. spiralis ML ESPs significantly inhibited H446 cell proliferation, which was dose-and time-dependent. The results of flow cytometry testing indicate a clear apoptosis trend in H446 cells co-cultured with ESPs for 24 h. Reverse transcription polymerase chain reaction and Western blotting results showed increased expression of pro-apoptosis genes Bax, Cyt-C, Apaf-1, caspase-9 and caspase-3, compared with the negative control group, and decreased the expression of anti-apoptosis genes Bcl-2 and Livin. Our results suggest that T. spiralis ML ESPs can induce apoptosis in H446 cells through a mitochondrial pathway, which may be a mechanism of antineoplastic action in T. spiralis ML ESPs.
authors.affiliation: Department of Pathogenic Biology,Chengde Medical University,Chengde 06700,Hebei,China.
authors.forename: Jingmei
authors.initials: J
authors.lastname: Luo
authors.affiliation: Department of Pathogenic Biology,Chengde Medical University,Chengde 06700,Hebei,China.
authors.forename: Li
authors.initials: L
authors.lastname: Yu
authors.affiliation: Department of Pathogenic Biology,Chengde Medical University,Chengde 06700,Hebei,China.
authors.forename: Guangcheng
authors.initials: G
authors.lastname: Xie
authors.affiliation: Department of Pathogenic Biology,Chengde Medical University,Chengde 06700,Hebei,China.
authors.forename: Dan
authors.initials: D
authors.lastname: Li
authors.affiliation: Department of Pathogenic Biology,Chengde Medical University,Chengde 06700,Hebei,China.
authors.forename: Meng
authors.initials: M
authors.lastname: Su
authors.affiliation: Department of Immunology,Chengde Medical University,Chengde 06700,Hebei,China.
authors.forename: Xuerong
authors.initials: X
authors.lastname: Zhao
authors.affiliation: Department of Pathogenic Biology,Chengde Medical University,Chengde 06700,Hebei,China.
authors.forename: Luanying
authors.initials: L
authors.lastname: Du
mesh_terms: D000818:Animals; D000947:Antigens, Helminth / Q000276:immunology / Q000502:physiology*; D017209:Apoptosis / Q000502:physiology*; D049109:Cell Proliferation; D015801:Helminth Proteins / Q000276:immunology / Q000502:physiology*; D007814:Larva; D008175:Lung Neoplasms / Q000473:pathology*; D051379:Mice; D008928:Mitochondria / Q000473:pathology*; D009132:Muscles / Q000469:parasitology; D012333:RNA, Messenger / Q000378:metabolism; D055752:Small Cell Lung Carcinoma / Q000473:pathology*; D017160:Trichinella spiralis / Q000235:genetics / Q000502:physiology*; D014407:Tumor Cells, Cultured
chemical_list: D000947:Antigens, Helminth; D015801:Helminth Proteins; D012333:RNA, Messenger; C097419:excretory-secretory antigen, Trichinella
keywords: 
                 Trichinella spiralis
              ; Apoptosis; excretory–secretory proteins; mitochondrial pathway; small cell lung cancer