title: Nitrogen Fertilizer and Straw Applications Affect Uptake of 13C,15N-Glycine by Soil Microorganisms in Wheat Growth Stages.
abstract: This study investigated the influence of nitrogen (N) fertilizer and straw on intact amino acid N uptake by soil microorganisms and the relationship between amino acid turnover and soil properties during the wheat growing season. A wheat pot experiment was carried out with three treatments: control (CK), N fertilizer (NF) and N fertilizer plus rice straw (NS). We used stable isotope compound-specific analysis to determine the uptake of 13C,15N-glycine by soil microorganisms. In the NF treatment, microbial 13C,15N-glycine uptake was lower compared with CK, suggesting that inorganic N was the preferred N source for soil microorganisms. However, The application of straw with N fertilizer (in NS treatment) increased microbial 13C,15N-glycine uptake even with the same amount of N fertilizer application. In this treatment, enzyme activities, soil microbial biomass C and microbial biomass N increased simultaneously because more C was available. Soil mineral N and plant N contents all decreased substantially. The increased uptake of intact 13C,15N-glycine in the NS treatment can be attributed to direct assimilation by soil microorganisms to satisfy the demand for N when inorganic N was consumed.
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.|Graduate School, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Lijie
authors.initials: L
authors.lastname: Yang
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.
authors.forename: Lili
authors.initials: L
authors.lastname: Zhang
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.|Graduate School, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Chunxiao
authors.initials: C
authors.lastname: Yu
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.
authors.forename: Dongpo
authors.initials: D
authors.lastname: Li
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Gong
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xue
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.
authors.forename: Yuchao
authors.initials: Y
authors.lastname: Song
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.|Graduate School, University of Chinese Academy of Sciences, Beijing, China.
authors.forename: Yalan
authors.initials: Y
authors.lastname: Cui
authors.affiliation: Department of Land, Air, and Water Resources, University of California Davis, Davis California, United States of America.
authors.forename: Timothy A
authors.initials: TA
authors.lastname: Doane
authors.affiliation: National Nutrition and Engineering Lab, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang Liaoning, China.
authors.forename: Zhijie
authors.initials: Z
authors.lastname: Wu
mesh_terms: D000596:Amino Acids / Q000032:analysis; D001419:Bacteria / Q000378:metabolism*; D018533:Biomass; D002244:Carbon / Q000032:analysis; D002247:Carbon Isotopes; D005308:Fertilizers / Q000032:analysis*; D005998:Glycine / Q000378:metabolism*; D009584:Nitrogen / Q000032:analysis*; D012987:Soil / Q000737:chemistry; D012988:Soil Microbiology; D012996:Solutions; D014908:Triticum / Q000254:growth & development*
chemical_list: D000596:Amino Acids; D002247:Carbon Isotopes; D005308:Fertilizers; D012987:Soil; D012996:Solutions; D002244:Carbon; D009584:Nitrogen; D005998:Glycine