title: Preparation and characterization of novel functionalized multiwalled carbon nanotubes/chitosan/β-Glycerophosphate scaffolds for bone tissue engineering.
abstract: A major limitation in current tissue engineering scaffolds is that some of the most important characteristics of the intended tissue are ignored. As piezoelectricity and high mechanical strength are two of the most important characteristics of the bone tissue, carbon nanotubes are getting a lot of attention as a bone tissue scaffold component in recent years. In the present study, composite scaffolds comprised of functionalized Multiwalled Carbon Nanotubes (f-MWCNT), medium molecular weight chitosan and β-Glycerophosphate were fabricated and characterized. Biodegradability and mechanical tests indicate that while increasing f-MWCNT content can improve electrical conductivity and mechanical properties, there are some limitations for these increases, such as a decrease in mechanical properties and biodegradability in 1w/v% content of f-MWCNTs. Also, MTT cytotoxicity assay was conducted for the scaffolds and no significant cytotoxicity was observed. Increasing f-MWCNT content led to higher alkaline Phosphatase activity. The overall results show that composites with f-MWCNT content between 0.1w/v% and 0.5w/v% are the most suitable for bone tissue engineering application. Additionally, Preliminary cell electrical tests proved the efficiency of the prepared scaffolds for cell electrical applications.
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran 1316943551, Iran; Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran 1591634311, Iran.
authors.forename: Shayan
authors.initials: S
authors.lastname: Gholizadeh
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran 1591634311, Iran.
authors.forename: Fathollah
authors.initials: F
authors.lastname: Moztarzadeh
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran 1316943551, Iran.
authors.forename: Nooshin
authors.initials: N
authors.lastname: Haghighipour
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran 1316943551, Iran.
authors.forename: Leila
authors.initials: L
authors.lastname: Ghazizadeh
authors.affiliation: Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran 1591634311, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Baghbani
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran 1316943551, Iran. Electronic address: mashokrgozar@pasteur.ac.ir.
authors.forename: Mohammad Ali
authors.initials: MA
authors.lastname: Shokrgozar
authors.affiliation: National Cell Bank of Iran, Pasteur Institute of Iran, Tehran 1316943551, Iran; Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran 1591634311, Iran. Electronic address: Allahyari.aut@gmail.com.
authors.forename: Zahra
authors.initials: Z
authors.lastname: Allahyari
mesh_terms: D000469:Alkaline Phosphatase / Q000378:metabolism; D001672:Biocompatible Materials / Q000737:chemistry* / Q000494:pharmacology / Q000633:toxicity; D001842:Bone and Bones / Q000166:cytology* / Q000187:drug effects; D002460:Cell Line; D048271:Chitosan / Q000737:chemistry*; D019245:Compressive Strength; D004553:Electric Conductivity; D005240:Feasibility Studies; D005994:Glycerophosphates / Q000737:chemistry*; D006801:Humans; D008422:Materials Testing; D037742:Nanotubes, Carbon / Q000737:chemistry*; D016062:Porosity; D013718:Tensile Strength; D023822:Tissue Engineering; D054457:Tissue Scaffolds / Q000737:chemistry*; D014867:Water / Q000737:chemistry
chemical_list: D001672:Biocompatible Materials; D005994:Glycerophosphates; D037742:Nanotubes, Carbon; D014867:Water; D048271:Chitosan; D000469:Alkaline Phosphatase; C031463:beta-glycerophosphoric acid
keywords: Bone tissue engineering; Carbon nanotubes; Chitosan; Conductive scaffolds; Freeze-drying method; β-Glycerophosphate