title: MicroRNA-638 inhibits cell proliferation by targeting suppress PIM1 expression in human osteosarcoma.
abstract: MicroRNAs (miRNAs) are a type of small noncoding RNAs that often play important roles in carcinogenesis, but the carcinogenic mechanism of miRNAs is still unclear. This study will investigate the functions and the mechanism of miR-638 in osteosarcoma (OS). The expression of miR-638 in OS and the DNA copy number of miR-638 were detected by real-time PCR. The effect of miR-638 on cell proliferation was measured by CCK8 assay. Different assays, including bioinformatics algorithms, luciferase report assay, and Western blotting, were used to identify the target gene proviral integration site for Moloney murine leukemia virus 1 (PIM1) of miR-638 in OS. The expression of PIM1 in clinical OS tissues was also validated by immunohistochemical assay. From this research, we found that miR-638 was downregulated in OS tissues compared with corresponding noncancerous tissues (NCTs), and the DNA copy number of miR-638 was lower in OS than in NCTs, which may induce the corresponding downregulation of miR-638 in OS. Ectopic expression of miR-638 inhibited OS cell growth in vitro. Subsequently, we identified that PIM1 is the downstream target gene of miR-638 in OS cells, and silencing PIM1 expression phenocopied the inhibitory effect of miR-638 on OS cell proliferation. Furthermore, we observed that PIM1 was overexpressed in OS tissues, and high expression of PIM1 in OS predicted poor overall survival. In summary, we revealed that miR-638 functions as a tumor suppressor through inhibiting PIM1 expression in OS.
authors.affiliation: Department of Joint Surgery, the Second Affiliated Hospital, University of South China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China.
authors.forename: Xiao-Xu
authors.initials: XX
authors.lastname: Wang
authors.affiliation: Department of Dobstertics and Gynecology, the Second Affiliated Hospital, University of South China, Hengyang, Hunan, People's Republic of China.
authors.forename: Jue
authors.initials: J
authors.lastname: Liu
authors.affiliation: Department of Nursing, the First Affiliated Hospital, University of South China, Hengyang, Hunan, People's Republic of China.
authors.forename: Yi-Min
authors.initials: YM
authors.lastname: Tang
authors.affiliation: Department of Joint Surgery, the Second Affiliated Hospital, University of South China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China.
authors.forename: Liang
authors.initials: L
authors.lastname: Hong
authors.affiliation: Department of Joint Surgery, the Second Affiliated Hospital, University of South China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China.
authors.forename: Zhi
authors.initials: Z
authors.lastname: Zeng
authors.affiliation: Department of Joint Surgery, the Second Affiliated Hospital, University of South China, 35 Jiefang Road, Hengyang, Hunan, People's Republic of China. fueryisheng@163.com.
authors.forename: Guang-Hua
authors.initials: GH
authors.lastname: Tan
keywords: Osteosarcoma; PIM1; Proliferation; miR-638