title: Distribution of Suicin Gene Clusters inStreptococcus suisSerotype 2 Belonging to Sequence Types 25 and 28.
abstract: Recently, we reported the purification and characterization of three distinct lantibiotics (named suicin 90-1330, suicin 3908, and suicin 65) produced byStreptococcus suis. In this study, we investigated the distribution of the three suicin lantibiotic gene clusters among serotype 2S. suisstrains belonging to sequence type (ST) 25 and ST28, the two dominant STs identified in North America. The genomes of 102 strains were interrogated for the presence of suicin gene clusters encoding suicins 90-1330, 3908, and 65. The gene cluster encoding suicin 65 was the most prevalent and mainly found among ST25 strains. In contrast, none of the genes related to suicin 90-1330 production were identified in 51 ST25 strains nor in 35/51 ST28 strains. However, the complete suicin 90-1330 gene cluster was found in ten ST28 strains, although some genes in the cluster were truncated in three of these isolates. The vast majority (101/102) ofS. suisstrains did not possess any of the genes encoding suicin 3908. In conclusion, this study indicates heterogeneous distribution of suicin genes inS. suis.
authors.affiliation: Public Health Ontario Laboratory, Toronto, ON, Canada.
authors.forename: Taryn B T
authors.initials: TB
authors.lastname: Athey
authors.affiliation: Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada.
authors.forename: Katy
authors.initials: K
authors.lastname: Vaillancourt
authors.affiliation: Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada; Centre de Recherche en Infectiologie Porcine et Avicole (CRIPA), Fonds de Recherche du Québec-Nature et Technologies (FQRNT), Saint-Hyacinthe, QC, Canada.
authors.forename: Michel
authors.initials: M
authors.lastname: Frenette
authors.affiliation: Public Health Ontario Laboratory, Toronto, ON, Canada; Department of Laboratory Medicine and Pathobiology, Faculty of Medicine, University of Toronto, Toronto, ON, Canada.
authors.forename: Nahuel
authors.identifier: 0000-0001-9553-3798
authors.initials: N
authors.lastname: Fittipaldi
authors.affiliation: Centre de Recherche en Infectiologie Porcine et Avicole (CRIPA), Fonds de Recherche du Québec-Nature et Technologies (FQRNT), Saint-Hyacinthe, QC, Canada; Groupe de Recherche sur les Maladies Infectieuses du Porc (GREMIP), Faculté de Médecine Vétérinaire, Université de Montréal, Saint-Hyacinthe, QC, Canada.
authors.forename: Marcelo
authors.initials: M
authors.lastname: Gottschalk
authors.affiliation: Groupe de Recherche en Écologie Buccale (GREB), Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada; Centre de Recherche en Infectiologie Porcine et Avicole (CRIPA), Fonds de Recherche du Québec-Nature et Technologies (FQRNT), Saint-Hyacinthe, QC, Canada.
authors.forename: Daniel
authors.identifier: 0000-0003-0878-4674
authors.initials: D
authors.lastname: Grenier
mesh_terms: D000818:Animals; D001426:Bacterial Proteins / Q000235:genetics*; D001430:Bacteriocins / Q000096:biosynthesis / Q000235:genetics*; D016680:Genome, Bacterial; D005810:Multigene Family; D065288:Serogroup; D013290:Streptococcal Infections / Q000235:genetics* / Q000382:microbiology / Q000662:veterinary; D017011:Streptococcus suis / Q000235:genetics*; D013552:Swine
chemical_list: D001426:Bacterial Proteins; D001430:Bacteriocins; C000613511:Suicin protein, Streptococcus suis