title: SUMOylation of KLF4 promotes IL-4 induced macrophage M2 polarization.
abstract: Macrophages, in response to different environmental cues, undergo the classical polarization (M1 macrophages) as well as the alternative polarization (M2 macrophages) that involve the functions of stimulus-specific transcription factors. Kruppel-like factor 4 (KLF4), a member of a subfamily of the zinc-finger class of DNA-binding transcription factors, plays as a critical regulator of macrophage polarization. KLF4 has been reported as a SUMOylated protein. In this study, we showed that SUMOylation of KLF4, is induced by IL-4 treatment in macrophages. IL4-induced KLF4 SUMOylation promotes RAW264.7 cells and bone marrow derived macrophages (BMDMs) to polarize into M2 subset. Thus, we identified an important post-translational modification (PTM), SUMOylation, plays a crucial role in regulating KLF4 activity during IL-4 induced macrophage M2 polarization. SUMOylation of KLF4 can be a potential therapeutic target in the resolution of inflammation.
authors.affiliation: a Department of Biochemistry and Molecular Cell Biology , Shanghai Jiaotong University School of Medicine , Shanghai , China.|b Department of Pathophysiology , Dalian Medical University , Dalian , China.
authors.forename: Kezhou
authors.initials: K
authors.lastname: Wang
authors.affiliation: a Department of Biochemistry and Molecular Cell Biology , Shanghai Jiaotong University School of Medicine , Shanghai , China.
authors.forename: Wei
authors.initials: W
authors.lastname: Zhou
authors.affiliation: a Department of Biochemistry and Molecular Cell Biology , Shanghai Jiaotong University School of Medicine , Shanghai , China.|c Department of Clinical Laboratory , Ruijin Hospital, Shanghai Jiaotong University School of Medicine , Shanghai , China.
authors.forename: Qi
authors.initials: Q
authors.lastname: Cai
authors.affiliation: a Department of Biochemistry and Molecular Cell Biology , Shanghai Jiaotong University School of Medicine , Shanghai , China.
authors.forename: Jinke
authors.initials: J
authors.lastname: Cheng
authors.affiliation: a Department of Biochemistry and Molecular Cell Biology , Shanghai Jiaotong University School of Medicine , Shanghai , China.
authors.forename: Rong
authors.initials: R
authors.lastname: Cai
authors.affiliation: b Department of Pathophysiology , Dalian Medical University , Dalian , China.
authors.forename: Rong
authors.initials: R
authors.lastname: Xing
mesh_terms: D000818:Animals; D001119:Arginase / Q000235:genetics; D002460:Cell Line; D016764:Cell Polarity / Q000187:drug effects*; D006801:Humans; D015847:Interleukin-4 / Q000494:pharmacology*; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors / Q000378:metabolism*; D008264:Macrophages / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism*; D051379:Mice; D008954:Models, Biological; D011401:Promoter Regions, Genetic / Q000235:genetics; D011485:Protein Binding / Q000187:drug effects; D058207:Sumoylation / Q000187:drug effects*
chemical_list: C000715467:KLF4 protein, human; C000715468:Klf4 protein, mouse; D000090062:Kruppel-Like Factor 4; D051741:Kruppel-Like Transcription Factors; D015847:Interleukin-4; D001119:Arginase
keywords: IL-4; Kruppel-like factor 4 (KLF4); SUMOylation; alternative polarization; macrophage