title: Microfluidic DNA Stretching Device for Single-Molecule Diagnostics.
abstract: The method described here enables the automatic stretching and patterning of single DNA molecules onto a solid surface. It does not require chemical modification of the DNA or surface modification of the substrate. To detect a signal variation caused by sequence-specific dye binding or partial melting, it is crucial that the DNA molecules are arrayed in a parallel direction inside the narrow microscopic field. The method uses zigzag-shaped microgrooves in a densely-arranged molecular patterning apparatus in a microfluidic channel. By syringing through the microchannel, over 1500 DNA molecules can be arrayed simultaneously in the microgrooves. It will therefore serve as a template preparation for DNA molecular diagnosis by high-resolution imaging.
authors.affiliation: Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. onoshima-d@nanobio.nagoya-u.ac.jp.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan. onoshima-d@nanobio.nagoya-u.ac.jp.
authors.forename: Daisuke
authors.initials: D
authors.lastname: Onoshima
authors.affiliation: Institute of Innovation for Future Society, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.|ImPACT Research Center for Advanced Nanobiodevices, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.|Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8603, Japan.|Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14, Hayashi-cho, Takamatsu, 761-0395, Japan.
authors.forename: Yoshinobu
authors.initials: Y
authors.lastname: Baba
mesh_terms: D004247:DNA; D004867:Equipment Design; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000379:methods*; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D036103:Nanotechnology; D000072760:Single Molecule Imaging / Q000295:instrumentation / Q000379:methods*
chemical_list: D004247:DNA
keywords: DNA stretching; Microfluidic device; Molecular diagnosis; Optical mapping; Single-Molecule detection