title: Canine Central Nervous System Neoplasm Phenotyping Using Tissue Microarray Technique.
abstract: Tissue microarrays (TMAs) represent a useful technique for the simultaneous phenotyping of large sample numbers and are particularly suitable for histopathologic tumor research. In this study, TMAs were used to evaluate semiquantitatively the expression of multiple antigens in various canine central nervous system (CNS) neoplasms and to identify markers with potential discriminative diagnostic relevance. Ninety-seven canine CNS neoplasms, previously diagnosed on hematoxylin and eosin sections according to the World Health Organization classification, were investigated on TMAs, with each tumor consisting of 2 cylindrical samples from the center and the periphery of the neoplasm. Tumor cells were phenotyped using a panel of 28 monoclonal and polyclonal antibodies, and hierarchical clustering analysis was applied to group neoplasms according to similarities in their expression profiles. Hierarchical clustering generally grouped cases with similar histologic diagnoses; however, gliomas especially exhibited a considerable heterogeneity in their positivity scores. Multiple tumor groups, such as astrocytomas and oligodendrogliomas, significantly differed in the proportion of positive immunoreaction for certain markers such as p75NTR, AQP4, GFAP, and S100 protein. The study highlights AQP4 and p75NTRas novel markers, helping to discriminate between canine astrocytoma and oligodendroglioma. Furthermore, the results suggest that p75NTRand proteolipid protein may represent useful markers, whose expression inversely correlates with malignant transformation in canine astrocytomas and oligodendrogliomas, respectively. Tissue microarray was demonstrated to be a useful and time-saving tool for the simultaneous immunohistochemical characterization of multiple canine CNS neoplasms. The present study provides a detailed overview of the expression patterns of different types of canine CNS neoplasms.
authors.affiliation: 1 Department of Pathology, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.|2 Center for Systems Neuroscience, Hannover, Germany.
authors.forename: I
authors.initials: I
authors.lastname: Spitzbarth
authors.affiliation: 1 Department of Pathology, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.|2 Center for Systems Neuroscience, Hannover, Germany.
authors.forename: F
authors.initials: F
authors.lastname: Heinrich
authors.affiliation: 1 Department of Pathology, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.|2 Center for Systems Neuroscience, Hannover, Germany.
authors.forename: V
authors.initials: V
authors.lastname: Herder
authors.affiliation: 1 Department of Pathology, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.
authors.forename: T
authors.initials: T
authors.lastname: Recker
authors.affiliation: 1 Department of Pathology, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.
authors.forename: P
authors.initials: P
authors.lastname: Wohlsein
authors.affiliation: 1 Department of Pathology, University of Veterinary Medicine Hannover Foundation, Hannover, Germany.|2 Center for Systems Neuroscience, Hannover, Germany.
authors.forename: W
authors.initials: W
authors.lastname: Baumgärtner
mesh_terms: D000818:Animals; D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor; D002490:Central Nervous System / Q000473:pathology; D016543:Central Nervous System Neoplasms / Q000175:diagnosis / Q000473:pathology / Q000662:veterinary*; D004283:Dog Diseases / Q000175:diagnosis / Q000473:pathology*; D004285:Dogs; D010641:Phenotype; D046888:Tissue Array Analysis / Q000662:veterinary
chemical_list: D000911:Antibodies, Monoclonal; D014408:Biomarkers, Tumor
keywords: aquaporin 4; astrocytoma; central nervous system; dogs; hierarchical clustering; immunohistochemistry; neoplasia; oligodendroglioma; p75 neurotrophin receptor; periaxin; proteolipid protein; tissue microarray; tumor