title: Metabolic phenotype of clinical and environmentalMycobacterium aviumsubsp.hominissuisisolates.
abstract: BACKGROUND
  Mycobacterium aviumsubsp.hominissuis(MAH) is an emerging opportunistic human pathogen. It can cause pulmonary infections, lymphadenitis and disseminated infections in immuno-compromised patients. In addition, MAH is widespread in the environment, since it has been isolated from water, soil or dust. In recent years, knowledge on MAH at the molecular level has increased substantially. In contrast, knowledge of the MAH metabolic phenotypes remains limited.
  
  
  METHODS
  In this study, for the first time we analyzed the metabolic substrate utilization of ten MAH isolates, five from a clinical source and five from an environmental source. We used BIOLOG Phenotype MicroarrayTMtechnology for the analysis. This technology permits the rapid and global analysis of metabolic phenotypes.
  
  
  RESULTS
  The ten MAH isolates tested showed different metabolic patterns pointing to high intra-species diversity. Our MAH isolates preferred to use fatty acids such as Tween, caproic, butyric and propionic acid as a carbon source, and L-cysteine as a nitrogen source. Environmental MAH isolates resulted in being more metabolically active than clinical isolates, since the former metabolized more strongly butyric acid (p= 0.0209) and propionic acid (p= 0.00307).
  
  
  CONCLUSIONS
  Our study provides new insight into the metabolism of MAH. Understanding how bacteria utilize substrates during infection might help the developing of strategies to fight such infections.
authors.affiliation: Division 16, Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute , Berlin , Germany.
authors.forename: Andrea
authors.initials: A
authors.lastname: Sanchini
authors.affiliation: Institute of Microbiology and Epizootics, Free University Berlin , Berlin , Germany.
authors.forename: Flavia
authors.initials: F
authors.lastname: Dematheis
authors.affiliation: NG 1 Microbial Genomics, Robert Koch Institute , Berlin , Germany.
authors.forename: Torsten
authors.initials: T
authors.lastname: Semmler
authors.affiliation: Division 16, Mycotic and Parasitic Agents and Mycobacteria, Robert Koch Institute , Berlin , Germany.
authors.forename: Astrid
authors.initials: A
authors.lastname: Lewin
keywords: Biolog; Metabolism; Mycobacterium avium; OmniLog®; Phenotype microarray