title: The Markers of Glutamate Metabolism in Peripheral Blood Mononuclear Cells and Neurological Complications in Lung Cancer Patients.
abstract: Objective. To evaluate the involvement of glutamate metabolism in peripheral blood mononuclear cells (PBMC) in the development of neurological complications in lung cancer and during chemotherapy.Methods. The prospective study included 221 lung cancer patients treated with chemotherapeutics. Neurological status and cognitive functions were evaluated at baseline and after 6-month follow-up. Glutamate level, the activities of glutaminase- (GLS-) glutamate synthetizing enzyme, glutamate dehydrogenase (GDH), and glutamate decarboxylase catalyzing glutamate degradation were analyzed in PBMC and in sera of lung cancer patients by means of spectrophotometric and colorimetric methods.Results. Chemotherapy of lung neoplasms induced increase of glutamate content in PBMC and its concentration in serum increased the activity of GDH in PBMC and decreased activity of glutaminase in PBMC. The changes in glutamate metabolism markers were associated with initial manifestation of neurological deficit in lung cancer patients and with new symptoms, which appear as a complication of chemotherapy. Moreover, the analyzed parameters of glutamate control correlated with a spectrum of cognitive functions measures in lung cancer patients.Conclusion. We have demonstrated dysregulation in glutamate and glutamate metabolism controlling enzymes as promising indicators of risk for chemotherapy-induced neurological complications in lung cancer patients with particular emphasis on cognitive impairment.
authors.affiliation: Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, Poznan, Przybyszewskiego str. 49, 60-355 Poznan, Poland.
authors.forename: Slawomir
authors.identifier: 0000-0002-5602-4306
authors.initials: S
authors.lastname: Michalak
authors.affiliation: Department of Neurochemistry and Neuropathology, Poznan University of Medical Sciences, Poznan, Przybyszewskiego str. 49, 60-355 Poznan, Poland.
authors.forename: Joanna
authors.identifier: 0000-0002-7201-1951
authors.initials: J
authors.lastname: Rybacka-Mossakowska
authors.affiliation: Department of Neurology, Poznan University of Medical Sciences, Przybyszewskiego Str. 49, 60-355 Poznan, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Ambrosius
authors.affiliation: Department of Chemotherapy, Wielkopolska Cancer Center, Garbary Str. 15, 61-866 Poznan, Poland.
authors.forename: Joanna
authors.initials: J
authors.lastname: Gazdulska
authors.affiliation: Sub-Department of Diurnal Chemotherapy Wielkopolska Center of Pulmonology and Thoracosurgery of Eugenia and Janusz Zeyland, Szamarzewskiego 62, 60-569 Poznan, Poland.
authors.forename: Iwona
authors.initials: I
authors.lastname: Gołda-Gocka
authors.affiliation: Department of Neurology, Poznan University of Medical Sciences, Przybyszewskiego Str. 49, 60-355 Poznan, Poland.
authors.forename: Wojciech
authors.initials: W
authors.lastname: Kozubski
authors.affiliation: Department of Oncology, Poznan University of Medical Sciences, Poznan, Szamarzewskiego Str. 82/84, 60-569 Poznan, Poland.
authors.forename: Rodryg
authors.initials: R
authors.lastname: Ramlau
mesh_terms: D000368:Aged; D015415:Biomarkers / Q000097:blood / Q000378:metabolism; D002289:Carcinoma, Non-Small-Cell Lung / Q000097:blood* / Q000150:complications / Q000188:drug therapy; D060825:Cognitive Dysfunction / Q000097:blood* / Q000209:etiology; D005260:Female; D005968:Glutamate Decarboxylase / Q000097:blood; D005969:Glutamate Dehydrogenase / Q000097:blood; D018698:Glutamic Acid / Q000097:blood*; D005972:Glutaminase / Q000097:blood; D006801:Humans; D008175:Lung Neoplasms / Q000097:blood* / Q000150:complications / Q000188:drug therapy; D008297:Male; D008875:Middle Aged; D009000:Monocytes / Q000378:metabolism*
chemical_list: D015415:Biomarkers; D018698:Glutamic Acid; D005969:Glutamate Dehydrogenase; D005972:Glutaminase; D005968:Glutamate Decarboxylase