title: Practical Guidelines for Studies on Sandfly-Borne Phleboviruses: Part II: Important Points to Consider for Fieldwork and Subsequent Virological Screening.
abstract: In this series of review articles entitled "Practical guidelines for studies on sandfly-borne phleboviruses," the important points to be considered at the prefieldwork stage were addressed in part I, including parameters to be taken into account to define the geographic area for sand fly trapping and how to organize field collections. Here in part II, the following points have been addressed: (1) factors influencing the efficacy of trapping and the different types of traps with their respective advantages and drawbacks, (2) how to process the trapped sand flies in the field, and (3) how to process the sand flies in the virology laboratory. These chapters provide the necessary information for adopting the most appropriate procedures depending on the requirements of the study. In addition, practical information gathered through years of experience of translational projects is included to help newcomers to fieldwork studies.
authors.affiliation: 1 Division for Human Medicine, Austrian Agency for Health and Food Safety (AGES) , Vienna, Austria .
authors.forename: Hartwig
authors.initials: H
authors.lastname: Huemer
authors.affiliation: 2 Centre IRD, UMR MIVEGEC (IRD 224-CNRS 5290-Universite Montpellier) , Montpellier, France .
authors.forename: Jorian
authors.initials: J
authors.lastname: Prudhomme
authors.affiliation: 3 Centre for Vectors and Infectious Diseases Research, National Institute of Health Ricardo Jorge , Aguas de Moura, Portugal .
authors.forename: Fatima
authors.initials: F
authors.lastname: Amaro
authors.affiliation: 4 UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille University-IRD 190-INSERM 1207-EHESP) , Marseille, France .|5 Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille , Marseille, France .
authors.forename: Amal
authors.initials: A
authors.lastname: Baklouti
authors.affiliation: 6 GmbH , Ausservillgraten, Austria .
authors.forename: Gernot
authors.initials: G
authors.lastname: Walder
authors.affiliation: 7 EBAL-VERG Laboratories, Ecology Division, Department of Biology, Faculty of Science, Science and Engineering Institute, Hacettepe University , Ankara, Turkey .
authors.forename: Bulent
authors.initials: B
authors.lastname: Alten
authors.affiliation: 8 UMR BIPAR, Animal Health Laboratory , ANSES, Maisons-Alfort, France .
authors.forename: Sara
authors.initials: S
authors.lastname: Moutailler
authors.affiliation: 9 Department of Medical Microbiology, Faculty of Medicine, Hacettepe University , Ankara, Turkey .
authors.forename: Koray
authors.initials: K
authors.lastname: Ergunay
authors.affiliation: 4 UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille University-IRD 190-INSERM 1207-EHESP) , Marseille, France .|5 Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille , Marseille, France .|10 Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University , Jeddah, Saudi Arabia .
authors.forename: Remi N
authors.initials: RN
authors.lastname: Charrel
authors.affiliation: 4 UMR "Emergence des Pathologies Virales" (EPV: Aix-Marseille University-IRD 190-INSERM 1207-EHESP) , Marseille, France .|5 Fondation IHU Méditerranée Infection, APHM Public Hospitals of Marseille , Marseille, France .
authors.forename: Nazli
authors.initials: N
authors.lastname: Ayhan
mesh_terms: D063147:Animal Distribution; D000818:Animals; D006801:Humans; D007303:Insect Vectors / Q000821:virology*; D016856:Phlebovirus / Q000235:genetics / Q000302:isolation & purification / Q000502:physiology*; D010802:Phylogeny; D011576:Psychodidae / Q000145:classification / Q000821:virology*; D012107:Research Design
keywords: Bunyaviridae; Phlebotomus; Toscana virus; arbovirus; phlebovirus