title: Cytokinin Response Factor 5 has transcriptional activity governed by its C-terminal domain.
abstract: Cytokinin Response Factors (CRFs) are AP2/ERF transcription factors involved in cytokinin signal transduction. CRF proteins consist of a N-terminal dimerization domain (CRF domain), an AP2 DNA-binding domain, and a clade-specific C-terminal region of unknown function. Using a series of sequential deletions in yeast-2-hybrid assays, we provide evidence that the C-terminal region of Arabidopsis CRF5 can confer transactivation activity. Although comparative analyses identified evolutionarily conserved protein sequence within the C-terminal region, deletion experiments suggest that this transactivation domain has a partially redundant modular structure required for activation of target gene transcription.
authors.affiliation: a Department of Arctic and Marine Biology , UiT The Arctic University of Norway in Tromsø , Dramsvegen, Tromsø , Norway.|c ArcticZymes AS , Sykehusveien, Tromsø , Norway.
authors.forename: Bernd
authors.initials: B
authors.lastname: Striberny
authors.affiliation: b Department of Biological Sciences , Auburn University , Auburn , AL , USA.
authors.forename: Anthony E
authors.initials: AE
authors.lastname: Melton
authors.affiliation: a Department of Arctic and Marine Biology , UiT The Arctic University of Norway in Tromsø , Dramsvegen, Tromsø , Norway.
authors.forename: Rainer
authors.initials: R
authors.lastname: Schwacke
authors.affiliation: a Department of Arctic and Marine Biology , UiT The Arctic University of Norway in Tromsø , Dramsvegen, Tromsø , Norway.
authors.forename: Kirsten
authors.initials: K
authors.lastname: Krause
authors.affiliation: a Department of Arctic and Marine Biology , UiT The Arctic University of Norway in Tromsø , Dramsvegen, Tromsø , Norway.
authors.forename: Karsten
authors.initials: K
authors.lastname: Fischer
authors.affiliation: b Department of Biological Sciences , Auburn University , Auburn , AL , USA.
authors.forename: Leslie R
authors.initials: LR
authors.lastname: Goertzen
authors.affiliation: b Department of Biological Sciences , Auburn University , Auburn , AL , USA.
authors.forename: Aaron M
authors.initials: AM
authors.lastname: Rashotte
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant / Q000235:genetics / Q000502:physiology; D018398:Homeodomain Proteins / Q000235:genetics / Q000378:metabolism; D009687:Nuclear Proteins / Q000235:genetics / Q000378:metabolism; D000072417:Protein Domains / Q000235:genetics / Q000502:physiology; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism*
chemical_list: C499534:APETALA2 protein, Arabidopsis; D029681:Arabidopsis Proteins; D018398:Homeodomain Proteins; D009687:Nuclear Proteins; D014157:Transcription Factors
keywords: AP2 transcription factors; arabidopsis thaliana; cytokinin response factors; transactivation domain