title: Lack of correlation between X-ray repair cross-complementing group 1 gene polymorphisms and the susceptibility to colorectal cancer in a Malaysian cohort.
abstract: X-ray repair cross-complementing group 1 (XRCC1) is one of the key components in the base excision repair pathway that repairs erroneous DNA lesions and removes nonbulky base adducts for the maintenance of genome integrity. Studies have revealed that differences in individual DNA repair capacity can impact the interindividual variation in cancer susceptibility, tumour aggressiveness and treatment response. The relationship between XRCC1 and sporadic colorectal cancer (CRC) susceptibility, which is hitherto inconclusive, has been explored in many association studies of different populations. In view of the conflicting findings generated, we aimed to investigate the association between XRCC1 and genetic predisposition to CRC among Malaysians. The present case-control association study was conducted on 130 CRC patients and 212 age-matched healthy controls. The genotyping of XRCC1 Arg194Trp, Arg280His and Arg399Gln single nucleotide polymorphisms was performed with allele-specific real-time PCR approach. This was followed by basic statistical analysis on the single nucleotide polymorphisms and haplotype data obtained. No significant difference in the allele and genotype frequencies was observed between CRC patients and healthy controls (P>0.05). There was also no association observed between XRCC1 haplotypes and CRC (P>0.05). In conclusion, a positive association between XRCC1 gene polymorphisms and CRC risk was not established in our Malaysian population.
authors.affiliation: Departments of aPathology bBiomedical Science cSurgery dMedicine, Division of Gastroenterology and Hepatology, Faculty of Medicine, University of Malaya, Kuala Lumpur eCentre for Research & Innovation, Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, Kota Kinabalu, Sabah, Malaysia.
authors.forename: Tze-Pheng
authors.initials: TP
authors.lastname: Lau
authors.forename: Lay-Hoong
authors.initials: LH
authors.lastname: Lian
authors.forename: Phaik-Leng
authors.initials: PL
authors.lastname: Cheah
authors.forename: Lai-Meng
authors.initials: LM
authors.lastname: Looi
authors.forename: April C
authors.initials: AC
authors.lastname: Roslani
authors.forename: Khean-Lee
authors.initials: KL
authors.lastname: Goh
authors.forename: Ping-Chin
authors.initials: PC
authors.lastname: Lee
authors.forename: Kek-Heng
authors.initials: KH
authors.lastname: Chua
mesh_terms: D000328:Adult; D000368:Aged; D000369:Aged, 80 and over; D015331:Cohort Studies; D015179:Colorectal Neoplasms / Q000175:diagnosis / Q000453:epidemiology* / Q000235:genetics*; D005260:Female; D005819:Genetic Markers / Q000235:genetics; D020022:Genetic Predisposition to Disease / Q000453:epidemiology / Q000235:genetics*; D006801:Humans; D008296:Malaysia / Q000453:epidemiology; D008297:Male; D008875:Middle Aged; D020641:Polymorphism, Single Nucleotide / Q000235:genetics*; D000076105:X-ray Repair Cross Complementing Protein 1 / Q000235:genetics*
chemical_list: D005819:Genetic Markers; D000076105:X-ray Repair Cross Complementing Protein 1; C000619371:XRCC1 protein, human