title: Ferritin Protein Regulates the Degradation of Iron Oxide Nanoparticles.
abstract: Proteins implicated in iron homeostasis are assumed to be also involved in the cellular processing of iron oxide nanoparticles. In this work, the role of an endogenous iron storage protein-namely the ferritin-is examined in the remediation and biodegradation of magnetic iron oxide nanoparticles. Previous in vivo studies suggest the intracellular transfer of the iron ions released during the degradation of nanoparticles to endogenous protein cages within lysosomal compartments. Here, the capacity of ferritin cages to accommodate and store the degradation products of nanoparticles is investigated in vitro in the physiological acidic environment of the lysosomes. Moreover, it is questioned whether ferritin proteins can play an active role in the degradation of the nanoparticles. The magnetic, colloidal, and structural follow-up of iron oxide nanoparticles and proteins in lysosome-like medium confirms the efficient remediation of potentially harmful iron ions generated by nanoparticles within ferritins. The presence of ferritins, however, delays the degradation of particles due to a complex colloidal behavior of the mixture in acidic medium. This study exemplifies the important implications of intracellular proteins in processes of degradation and metabolization of iron oxide nanoparticles.
authors.affiliation: Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
authors.forename: Jeanne
authors.initials: J
authors.lastname: Volatron
authors.affiliation: Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
authors.forename: Florent
authors.initials: F
authors.lastname: Carn
authors.affiliation: Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
authors.forename: Jelena
authors.initials: J
authors.lastname: Kolosnjaj-Tabi
authors.affiliation: Laboratoire Matériaux et Phénomènes Quantiques, UMR 7162 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.|Department of Physics, University of Agriculture, Faisalabad, Pakistan.
authors.forename: Yasir
authors.initials: Y
authors.lastname: Javed
authors.affiliation: Service de Physique Biomédicale, Université de Mons, 20 Place du Parc, 7000, Mons, Belgium.
authors.forename: Quoc Lam
authors.initials: QL
authors.lastname: Vuong
authors.affiliation: Service de Physique Biomédicale, Université de Mons, 20 Place du Parc, 7000, Mons, Belgium.
authors.forename: Yves
authors.initials: Y
authors.lastname: Gossuin
authors.affiliation: Laboratoire PHENIX, UMR 7195, CNRS/Université Pierre et Marie Curie/ESPCI, 4 place Jussieu, 75252, Paris Cedex 05, France.
authors.forename: Christine
authors.initials: C
authors.lastname: Ménager
authors.affiliation: Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Luciani
authors.affiliation: Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
authors.forename: Gaëlle
authors.initials: G
authors.lastname: Charron
authors.affiliation: ITODYS, Interfaces, Traitements, Organisation et Dynamique des Systèmes, UMR 7086 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 15 rue Jean-Antoine de Baïf, 75205, Paris Cedex 13, France.
authors.forename: Miryana
authors.initials: M
authors.lastname: Hémadi
authors.affiliation: Laboratoire Matériaux et Phénomènes Quantiques, UMR 7162 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
authors.forename: Damien
authors.initials: D
authors.lastname: Alloyeau
authors.affiliation: Laboratoire Matières et Systèmes Complexes, UMR 7057 CNRS/Université Paris Diderot, Sorbonne Paris Cité, 10 rue Alice Domon et Léonie Duquet, 75205, Paris Cedex 13, France.
authors.forename: Florence
authors.initials: F
authors.lastname: Gazeau
mesh_terms: D000143:Acids / Q000737:chemistry; D000818:Animals; D001052:Apoferritins / Q000378:metabolism; D005290:Ferric Compounds / Q000737:chemistry*; D005293:Ferritins / Q000378:metabolism*; D006736:Horses; D006863:Hydrogen-Ion Concentration; D007700:Kinetics; D008247:Lysosomes / Q000378:metabolism; D060328:Magnetic Phenomena; D008670:Metals / Q000737:chemistry; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D053838:Scattering, Small Angle; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D000143:Acids; D005290:Ferric Compounds; D008670:Metals; C000499:ferric oxide; D005293:Ferritins; D001052:Apoferritins
keywords: biodegradation; ferritin; iron metabolism; iron oxide nanoparticles; nanomagnetism