title: A link between transcription fidelity and pausing in vivo.
abstract: Pausing by RNA polymerase is a major mechanism that regulates transcription elongation but can cause conflicts with fellow RNA polymerases and other cellular machineries. Here, we summarize our recent finding that misincorporation could be a major source of transcription pausing in vivo, and discuss the role of misincorporation-induced pausing.
authors.affiliation: a Centre for Bacterial Cell Biology , Institute for Cell and Molecular Biosciences, Newcastle University , Newcastle upon Tyne , UK.
authors.forename: Pamela
authors.initials: P
authors.lastname: Gamba
authors.affiliation: a Centre for Bacterial Cell Biology , Institute for Cell and Molecular Biosciences, Newcastle University , Newcastle upon Tyne , UK.
authors.forename: Katherine
authors.initials: K
authors.lastname: James
authors.affiliation: a Centre for Bacterial Cell Biology , Institute for Cell and Molecular Biosciences, Newcastle University , Newcastle upon Tyne , UK.
authors.forename: Nikolay
authors.initials: N
authors.lastname: Zenkin
mesh_terms: D012321:DNA-Directed RNA Polymerases / Q000378:metabolism*; D004926:Escherichia coli / Q000378:metabolism; D029968:Escherichia coli Proteins / Q000235:genetics / Q000378:metabolism; D061805:Transcription Elongation, Genetic / Q000502:physiology*; D014157:Transcription Factors / Q000235:genetics / Q000378:metabolism; D035602:Transcriptional Elongation Factors / Q000235:genetics / Q000378:metabolism
chemical_list: D029968:Escherichia coli Proteins; C079668:GreA protein, E coli; C079667:GreB protein, E coli; D014157:Transcription Factors; D035602:Transcriptional Elongation Factors; C090751:dksA protein, E coli; D012321:DNA-Directed RNA Polymerases
keywords: Gre; TFIIS; collisions of transcription with replication; fidelity; misincorporation; pausing; proofreading; transcription