title: Epigallocatechin-3-gallate Modulates MicroRNA Expression Profiles in Human Nasopharyngeal Carcinoma CNE2 Cells.
abstract: BACKGROUND
  Epigallocatechin-3-gallate (EGCG) has exhibited antitumor properties in several types of cancers, including nasopharyngeal carcinoma (NPC), but the molecular mechanisms underlying this function remain incompletely understood. The aim of the present study was to characterize the global impact of EGCG on the expression of microRNAs (miRNAs) in NPC cells.
  
  
  METHODS
  Using microarray analysis, the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells. Furthermore, the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis.
  
  
  RESULTS
  A total of 14 miRNAs exhibited >2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG. Totally 43, 49, and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis, cell cycle regulation, and cell proliferation, respectively. A total of 66 signaling pathways, primarily involved in cancer development and lipid and glucose metabolism, were shown to be regulated by EGCG-specific miRNAs.
  
  
  CONCLUSIONS
  EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells, which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG.
authors.affiliation: Department of Pathophysiology, Guangdong Medical University, Dongguan, Guangdong 523808; Sino-American Cancer Research Institute, Key Laboratory for Medical Diagnostics of Guangdong Province, Guangdong Medical University, Dongguan, Guangdong 523808, China.
authors.forename: Bin-Bin
authors.initials: BB
authors.lastname: Li
authors.affiliation: Sino-American Cancer Research Institute, Key Laboratory for Medical Diagnostics of Guangdong Province, Guangdong Medical University, Dongguan, Guangdong 523808, China.
authors.forename: Guo-Liang
authors.initials: GL
authors.lastname: Huang
authors.affiliation: Sino-American Cancer Research Institute, Key Laboratory for Medical Diagnostics of Guangdong Province, Guangdong Medical University, Dongguan, Guangdong 523808, China.
authors.forename: Hua-Hui
authors.initials: HH
authors.lastname: Li
authors.affiliation: Department of Pathophysiology, Guangdong Medical University, Dongguan, Guangdong 523808, China.
authors.forename: Xia
authors.initials: X
authors.lastname: Kong
authors.affiliation: Department of Pathophysiology, Guangdong Medical University, Dongguan, Guangdong 523808; Sino-American Cancer Research Institute, Key Laboratory for Medical Diagnostics of Guangdong Province, Guangdong Medical University, Dongguan, Guangdong 523808, China.
authors.forename: Zhi-Wei
authors.initials: ZW
authors.lastname: He
mesh_terms: D000970:Antineoplastic Agents / Q000494:pharmacology; D002277:Carcinoma; D002392:Catechin / Q000031:analogs & derivatives* / Q000494:pharmacology; D045744:Cell Line, Tumor; D019295:Computational Biology; D015870:Gene Expression / Q000187:drug effects / Q000235:genetics; D006801:Humans; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism*; D000077274:Nasopharyngeal Carcinoma; D009303:Nasopharyngeal Neoplasms / Q000235:genetics*; D060888:Real-Time Polymerase Chain Reaction; D015398:Signal Transduction / Q000187:drug effects / Q000235:genetics
chemical_list: D000970:Antineoplastic Agents; D035683:MicroRNAs; D002392:Catechin; C045651:epigallocatechin gallate