title: Bacteria isolated from lung modulate asthma susceptibility in mice.
abstract: Asthma is a chronic, non-curable, multifactorial disease with increasing incidence in industrial countries. This study evaluates the direct contribution of lung microbial components in allergic asthma in mice. Germ-Free and Specific-Pathogen-Free mice display similar susceptibilities to House Dust Mice-induced allergic asthma, indicating that the absence of bacteria confers no protection or increased risk to aeroallergens. In early life, allergic asthma changes the pattern of lung microbiota, and lung bacteria reciprocally modulate aeroallergen responsiveness. Primo-colonizing cultivable strains were screened for their immunoregulatory properties following their isolation from neonatal lungs. Intranasal inoculation of lung bacteria influenced the outcome of allergic asthma development: the strain CNCM I 4970 exacerbated some asthma features whereas the pro-Th1 strain CNCM I 4969 had protective effects. Thus, we confirm that appropriate bacterial lung stimuli during early life are critical for susceptibility to allergic asthma in young adults.
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Aude
authors.identifier: 0000-0001-9896-3216
authors.initials: A
authors.lastname: Remot
authors.affiliation: VIM, INRA, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Delphyne
authors.initials: D
authors.lastname: Descamps
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Marie-Louise
authors.initials: ML
authors.lastname: Noordine
authors.affiliation: Animal Genetics and Integrative Biology (UMR1313, GABI), INRA-AgroParisTech, Jouy-en-Josas, France.
authors.forename: Abdelhak
authors.initials: A
authors.lastname: Boukadiri
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Elliot
authors.initials: E
authors.lastname: Mathieu
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Véronique
authors.initials: V
authors.lastname: Robert
authors.affiliation: VIM, INRA, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Sabine
authors.initials: S
authors.lastname: Riffault
authors.affiliation: Inflammation Research Center, VIB, Ghent, Belgium.|Department of Respiratory Medicine, Ghent University, Ghent, Belgium.
authors.forename: Bart
authors.initials: B
authors.lastname: Lambrecht
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Philippe
authors.initials: P
authors.lastname: Langella
authors.affiliation: Inflammation Research Center, VIB, Ghent, Belgium.|Department of Respiratory Medicine, Ghent University, Ghent, Belgium.
authors.forename: Hamida
authors.initials: H
authors.lastname: Hammad
authors.affiliation: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France.
authors.forename: Muriel
authors.identifier: 0000-0002-7608-3274
authors.initials: M
authors.lastname: Thomas
mesh_terms: D000485:Allergens; D000818:Animals; D001249:Asthma / Q000382:microbiology*; D001419:Bacteria / Q000302:isolation & purification; D018407:Bacterial Physiological Phenomena; D004198:Disease Susceptibility; D004391:Dust / Q000276:immunology; D008168:Lung / Q000382:microbiology*; D051379:Mice; D008810:Mice, Inbred C57BL
chemical_list: D000485:Allergens; D004391:Dust