title: Nanoparticle delivery of Wnt-1 siRNA enhances photodynamic therapy by inhibiting epithelial-mesenchymal transition for oral cancer.
abstract: Activation of the epithelial to mesenchymal transition (EMT) in photodynamic therapy (PDT) can lead to the recurrence and progression of tumors. To enhance the effects of PDT, it is essential to inhibit the Wnt/β-catenin signaling pathway involved in EMT progression. Herein, we used polyethylene glycol-polyethyleneimine-chlorin e6 (PEG-PEI-Ce6) nanoparticles to efficiently deliver Wnt-1 small interfering RNA (siRNA) to the cytoplasm of KB cells (oral squamous cell carcinoma) that were subjected to PDT. Wnt-1 siRNA effectively inhibited the Wnt/β-catenin signaling pathway, reducing the expression of Wnt-1, β-catenin and vimentin that are crucial to the EMT. Combined with Wnt-1 siRNA, PEG-PEI-Ce6 nanoparticle mediated PDT inhibited cell growth and enhanced the cancer cell killing effect remarkably. Our results show the promise of combination therapy of PEG-PEI-Ce6 nanoparticles for delivery of Wnt-1 siRNA along with PDT in the treatment of oral cancer.
authors.affiliation: Ninth People's Hospital Affiliated to Shanghai Jiao Tong University Medical College, School of Stomatology, Department of Oral Maxillofacial Surgery, Shanghai Institute of Stomatology, Shanghai Key Point Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai 200011, P. R. China. guoyuzhou@hotmail.com.
authors.forename: Chuan
authors.initials: C
authors.lastname: Ma
authors.affiliation: School of Chemistry and Chemical Engineering, Shanghai Key Lab of Electrical Insulation and Thermal Aging, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China. xyzhu@sjtu.edu.cn.
authors.forename: Leilei
authors.initials: L
authors.lastname: Shi
authors.affiliation: School of Chemistry and Chemical Engineering, Shanghai Key Lab of Electrical Insulation and Thermal Aging, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China. xyzhu@sjtu.edu.cn.
authors.forename: Yu
authors.initials: Y
authors.lastname: Huang
authors.affiliation: Ninth People's Hospital Affiliated to Shanghai Jiao Tong University Medical College, School of Stomatology, Department of Oral Maxillofacial Surgery, Shanghai Institute of Stomatology, Shanghai Key Point Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai 200011, P. R. China. guoyuzhou@hotmail.com.
authors.forename: Lingyue
authors.initials: L
authors.lastname: Shen
authors.affiliation: Ninth People's Hospital Affiliated to Shanghai Jiao Tong University Medical College, School of Stomatology, Department of Oral Maxillofacial Surgery, Shanghai Institute of Stomatology, Shanghai Key Point Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai 200011, P. R. China. guoyuzhou@hotmail.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Peng
authors.affiliation: School of Chemistry and Chemical Engineering, Shanghai Key Lab of Electrical Insulation and Thermal Aging, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, P. R. China. xyzhu@sjtu.edu.cn.
authors.forename: Xinyuan
authors.identifier: http://orcid.org/0000-0002-2891-837X
authors.initials: X
authors.lastname: Zhu
authors.affiliation: Ninth People's Hospital Affiliated to Shanghai Jiao Tong University Medical College, School of Stomatology, Department of Oral Maxillofacial Surgery, Shanghai Institute of Stomatology, Shanghai Key Point Laboratory of Stomatology, 639 Zhizaoju Road, Shanghai 200011, P. R. China. guoyuzhou@hotmail.com.
authors.forename: Guoyu
authors.initials: G
authors.lastname: Zhou
mesh_terms: D045744:Cell Line, Tumor; D002735:Chlorophyllides; D058750:Epithelial-Mesenchymal Transition; D006801:Humans; D009062:Mouth Neoplasms / Q000235:genetics / Q000473:pathology / Q000628:therapy*; D053758:Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D010778:Photochemotherapy / Q000379:methods*; D011092:Polyethylene Glycols / Q000737:chemistry; D011094:Polyethyleneimine / Q000031:analogs & derivatives / Q000737:chemistry; D011166:Porphyrins / Q000737:chemistry; D034741:RNA, Small Interfering / Q000008:administration & dosage / Q000235:genetics / Q000627:therapeutic use*; D000067492:RNAi Therapeutics / Q000379:methods*; D051155:Wnt1 Protein / Q000235:genetics*
chemical_list: D002735:Chlorophyllides; D011166:Porphyrins; D034741:RNA, Small Interfering; D051155:Wnt1 Protein; C544016:poly(ethylene glycol)-co-poly(ethyleneimine); D011092:Polyethylene Glycols; C062985:phytochlorin; D011094:Polyethyleneimine