title: Microscopy based studies on the interaction of bio-based silver nanoparticles with Bombyx mori Nuclear Polyhedrosis virus.
abstract: In the present investigation, silver nanoparticles (AgNPs) interactions with Bombyx mori Nuclear Polyhedrosis virus (BmNPV) were characterized using High-Resolution Scanning Electron Microscopy (HR-SEM), Energy Dispersive X-ray Analysis (EDAX), Transmission Electron Microscopy (TEM), Atomic Force Microcopy (AFM) and Confocal Microscope (CM). HR-SEM study reveals that the biosynthesized AgNPs have interacted with BmNPV and were found on the surface. TEM micrographs of normal and viral polyhedra treated with AgNPs showed that the nanoparticles were accumulated in the membrane and it was noted that some of the AgNPs successfully penetrated the membrane by reaching the capsid of BmNPV. AFM and confocal microscopy studies reveal that the disruption in the shell membrane tends to lose its stability due to exposure of AgNPs to BmNPV.
authors.affiliation: Department of Zoology, Thiruvalluvar University, Vellore 632 115, India. Electronic address: tamilphd85@gmail.com.
authors.forename: Selvaraj
authors.initials: S
authors.lastname: Tamilselvan
authors.affiliation: Department of Chemical Engineering, Indian Institute of Technology Madras, Chennai 600 026, India.
authors.forename: Thirunavukkarasu
authors.initials: T
authors.lastname: Ashokkumar
authors.affiliation: Centre for Ocean Research, Sathyabama University, Chennai 600 119, India.
authors.forename: Kasivelu
authors.initials: K
authors.lastname: Govindaraju
mesh_terms: D000818:Animals; D012831:Bombyx / Q000821:virology*; D053768:Metal Nanoparticles / Q000737:chemistry; D018625:Microscopy, Atomic Force; D018613:Microscopy, Confocal; D008855:Microscopy, Electron, Scanning; D046529:Microscopy, Electron, Transmission; D017924:Nucleopolyhedroviruses / Q000187:drug effects / Q000302:isolation & purification / Q000378:metabolism / Q000648:ultrastructure*; D012834:Silver / Q000494:pharmacology; D053545:Spirulina / Q000378:metabolism
chemical_list: D012834:Silver
keywords: AFM; BmNPV; HR-SEM; Silver nanoparticles; TEM