title: The complete genome sequence ofCronobacter sakazakiiATCC 29544T, a food-borne pathogen, isolated from a child's throat.
abstract: BACKGROUND
  Cronobacter sakazakiiis an emerging opportunistic pathogen that is associated with rare but life-threatening cases of severe diseases: meningitis, necrotizing enterocolitis, and sepsis in premature and full-term infants. However, the pathogenesis mechanism of this pathogen remains largely unknown. To determine its pathogenesis at the genomic level, the genome ofC. sakazakiiATCC 29544Twas completely sequenced and analyzed.
  
  
  RESULTS
  The genomic DNA, containing a circular chromosome and three plasmids, is composed of 4,511,265 bp with a GC content of 56.71%, containing 4380 predicted open reading frames (ORFs), 22 rRNA genes, and 83 tRNA genes. The plasmids, designated pCSK29544_p1, pCSK29544_p2, and pCSK29544_p3, were 93,905-bp, 4938-bp, and 53,457-bp with GC contents of 57.02, 54.88, and 50.07%, respectively. They were also predicted to have 72, 6, and 57 ORFs without RNA genes.
  
  
  CONCLUSIONS
  The strain ATCC 29544Tgenome hasompAandibeB-homologouscusCgenes, probably associated with the invasion of human brain microvascular endothelial cells (BMECs). In addition, gene clusters for siderophore production (iucABCD/iutA) and the related transport system (eitCBAD) were detected in pCSK29544_p1 plasmid, indicating better iron uptake ability for survival. Furthermore, to survive under extremely dry condition like milk powder, this genome has gene clusters for biosynthesis of capsular proteins (CSK29544_00281-00284) and cellulose (CSK29544_01124-01127) for biofilm formation and a gene cluster for utilization of sialic acid in the milk (nanKTAR). The genome information ofC. sakazakiiATCC 29544Twould provide further understanding of its pathogenesis at the molecular level for the regulation of pathogenicity and the development of a rapid detection method using biomarkers.
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826 Republic of Korea.|Department of Applied Chemistry and Biological Engineering, Ajou University, 206 Worldcup-ro, Yeongtong-gu, Suwon, 16499 Republic of Korea.
authors.forename: Seongok
authors.initials: S
authors.lastname: Kim
authors.affiliation: Department of Food Science and Biotechnology and Institute of Life Science and Resources, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do, 17104 Republic of Korea.
authors.forename: You-Tae
authors.initials: YT
authors.lastname: Kim
authors.affiliation: Department of Applied Chemistry and Biological Engineering, Ajou University, 206 Worldcup-ro, Yeongtong-gu, Suwon, 16499 Republic of Korea.
authors.forename: Hyunjin
authors.initials: H
authors.lastname: Yoon
authors.affiliation: Department of Food Science and Biotechnology and Institute of Life Science and Resources, Kyung Hee University, 1732 Deogyeong-daero, Giheung-gu, Yongin-si, Gyeonggi-do, 17104 Republic of Korea.
authors.forename: Ju-Hoon
authors.initials: JH
authors.lastname: Lee
authors.affiliation: Department of Food and Animal Biotechnology, Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, and Center for Food and Bioconvergence, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, 08826 Republic of Korea.
authors.forename: Sangryeol
authors.initials: S
authors.lastname: Ryu
keywords: Complete genome sequence; Cronobacter sakazakii; Infant milk formula; Pathogenesis; Virulence factor