title: Evaluating the Contribution of Gut Microbiota to the Variation of Porcine Fatness with the Cecum and Fecal Samples.
abstract: Microbial community in gastrointestinal tract participates in the development of the obesity as well as quite a few metabolic diseases in human. However, there are few studies about the relationship between gut microbiota and porcine fatness. Here, we used high-throughput sequencing to perform 16S rRNA gene analysis in 256 cecum luminal samples fromErhualianpigs and 244 stools fromBamaxiangpigs, and adopted a two-part model statistical method to evaluate the association of gut microbes with porcine fatness. As the results, we identified a total of 6 and 108 operational taxonomic units (OTUs), and 9 and 10 bacterial taxa which showed significant associations with fatness traits in the stool and cecum samples, respectively. Cross-validation analysis indicated that gut microbiome showed the largest effect on abdominal adipose by explaining 2.73% phenotypic variance of abdominal fat weight. Significantly more fatness-associated OTUs were identified in the cecum samples than that in the stools, suggesting that cecum luminal samples were better used for identification of fatness-associated microbes than stools. The fatness-associated OTUs were mainly annotated toLachnospiraceae,Ruminococcaceae,Prevotella,Treponema, andBacteroides. These microbes have been reported to produce short-chain fatty acids by fermenting dietary indigested polysaccharide and pectin. The short-chain fatty acids can regulate host body energy homeostasis, protect host from inflammation and inhibit fat mass development. Our findings suggested that the gut microbiome may be an important factor modulating fatness in pigs.
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Maozhang
authors.initials: M
authors.lastname: He
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Shaoming
authors.initials: S
authors.lastname: Fang
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Xiaochang
authors.initials: X
authors.lastname: Huang
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Yuanzhang
authors.initials: Y
authors.lastname: Zhao
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Shanlin
authors.initials: S
authors.lastname: Ke
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Yang
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Zhuojun
authors.initials: Z
authors.lastname: Li
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Gao
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Congying
authors.initials: C
authors.lastname: Chen
authors.affiliation: State Key Laboratory of Pig Genetic Improvement and Production Technology, Jiangxi Agricultural University Nanchang, China.
authors.forename: Lusheng
authors.initials: L
authors.lastname: Huang
keywords: 16S rRNA gene; fatness; gut microbiome; swine; two-part model analysis