title: The effect ofZataria multifloraBoiss hydroalcoholic extract and fractions in pentylenetetrazole-induced kindling in mice.
abstract: OBJECTIVE
  At present, there are many antiepileptic drugs with a wide range of side effects on the human body. It was assumed thatZataria multiflora Boiss(Z. multiflora) with sedative, anti-spasmodic and anti-inflammatory activity may be effective in the treatment of epilepsy. The aim of the present study was to elucidate the effect ofZ. multiflorahydroalcoholic extract and its fraction extracts on pentylenetetrazole (PTZ)-induced chemical kindling.
  
  
  METHODS
  In this experimental study, eight separate groups of male albino mice were used. All groups received 11 separate intraperitoneal injections of PTZ (35 mg/kg) with two-day intervals. 30 min before the injection of PTZ, mice received vehicle,Z. multiflorahydroalcoholic extract (300 and 600 mg/kg), n-hexane, acetone, methanol fraction extracts (150 mg/kg), or diazepam (10 mg/kg).
  
  
  RESULTS
  The kindled mice that were pretreated with vehicle showed a gradual increase in their seizure scores up to the end of the study. The hydroalcoholic extract ofZ. multiflora(300 and 600 mg/kg) reduced seizure scores significantly. However, n-hexane, acetone and methanol extracts did not affect seizure scores significantly.
  
  
  CONCLUSIONS
  The present findings demonstrate that the hydroalcoholic extract ofZ. multifloradid reduce the severity of seizure attacks in PTZ-induced chemical kindling in mice.
authors.affiliation: Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Shamsizadeh
authors.affiliation: Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Farangis
authors.initials: F
authors.lastname: Fatehi
authors.affiliation: Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Arab Baniasad
authors.affiliation: Physiology-Pharmacology Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.
authors.forename: Fatemeh
authors.initials: F
authors.lastname: Ayoobi
authors.affiliation: Department of Physiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
authors.forename: Mohammad Ebrahim
authors.initials: ME
authors.lastname: Rezvani
authors.affiliation: Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.
authors.forename: Ali
authors.initials: A
authors.lastname: Roohbakhsh
keywords: Pentylenetetrazole; Plant extracts; Seizure; Zataria multiflora Boiss