title: A Surface Pattern on MALDI Steel Plate for One-Step In-Situ Self-Desalting and Enrichment of Peptides/Proteins.
abstract: We report a novel strategy to achieve simultaneous one-step in-situ self-desalting and enrichment (OISE) of peptides/proteins on a facilely fabricated patterned MALDI steel plate with a circular paraffin-steel-polystyrene structure. The OISE plate could efficiently segregate salts from both analytes and matrices while retaining both analyte and matrix concentrate, and facilitating them to form homogeneous co-crystals on the centrally located polystyrene pattern. With the OISE plate, high quality and reproducible spectra could be obtained for low abundance peptides even in the presence of high salt concentrations (200 mM NH4HCO3, 1 M NaCl, or 400 mM urea). Using this strategy, a significant sensitivity enhancement was gained over traditional MALDI plate. The practical utility of this method was further demonstrated by the successful profiling of BSA digests and human serum. Graphical Abstract ᅟ.
authors.affiliation: College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Wang
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China. xiaocs@ciac.ac.cn.
authors.forename: Chunsheng
authors.initials: C
authors.lastname: Xiao
authors.affiliation: College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Li
authors.affiliation: College of Chemistry, Jilin University, Changchun, 130012, China.
authors.forename: Ling
authors.initials: L
authors.lastname: Ling
authors.affiliation: Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China.
authors.forename: Xuesi
authors.initials: X
authors.lastname: Chen
authors.affiliation: College of Chemistry, Jilin University, Changchun, 130012, China. guoxh@jlu.edu.cn.
authors.forename: Xinhua
authors.initials: X
authors.lastname: Guo
mesh_terms: D006801:Humans; D010455:Peptides / Q000032:analysis / Q000737:chemistry; D011137:Polystyrenes / Q000737:chemistry; D011506:Proteins / Q000032:analysis* / Q000737:chemistry*; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D044967:Serum / Q000737:chemistry; D012710:Serum Albumin, Bovine / Q000032:analysis / Q000737:chemistry; D012965:Sodium Chloride / Q000737:chemistry; D019032:Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / Q000295:instrumentation* / Q000379:methods*; D013232:Steel; D013499:Surface Properties
chemical_list: D010455:Peptides; D011137:Polystyrenes; D011506:Proteins; D013232:Steel; D012710:Serum Albumin, Bovine; D012965:Sodium Chloride
keywords: Desalting; Enrichment; MALDI; Surface pattern