title: Protective Effects of Pterostilbene Against Myocardial Ischemia/Reperfusion Injury in Rats.
abstract: Pterostilbene (PTB) has been suggested to protect against myocardial ischemia/reperfusion (MI/R) injury. Gas6/Axl signaling has been suggested to play an important role in cell survival. However, the interaction between PTB and Gas6/Axl signaling in MI/R remains unclear. This study aims to evaluate the role of Gas6/Axl signaling in the protective effects of PTB against MI/R injury. In experiment 1, the rats were subjected to 30 min of ischemia, followed by 3, 6, and 12 h of reperfusion, respectively. In experiment 2, the rats were administered intraperitoneally with PTB or vehicle and subjected to MI/R injury. The results suggested that the expression of Gas6 and Axl decreased significantly after MI/R injury. PTB treatment conferred a cardioprotective effect with an improved post-ischemic cardiac function, a reduced myocardial infarct size, and decreased lactate dehydrogenase and creatine kinase-MB in the serum, a decreased oxidative stress and inflammation, and a reduced number of apoptotic cardiomyocytes. Moreover, PTB treatment up-regulated the expression of Gas6, Axl, and Bcl-2 and down-regulated Bax expression. Our findings suggest that PTB treatment exerts cardioprotection against MI/R injury via attenuating inflammatory response, oxidative stress, and apoptosis and up-regulating the expression of Gas6 and Axl. The application of PTB may be a new strategy for the treatment of MI/R injury.
authors.affiliation: Department of Cardiology, Haikou People's Hospital, Xiangya School of Medicine Affiliated Haikou Hospital, Central South University, Haikou, 570208, People's Republic of China.|Department of Cardiology, Xiangya Hospital, Central South University, Changsha, 410078, People's Republic of China.
authors.forename: Miao
authors.initials: M
authors.lastname: Wu
authors.affiliation: Department of Cardiology, Haikou People's Hospital, Xiangya School of Medicine Affiliated Haikou Hospital, Central South University, Haikou, 570208, People's Republic of China.
authors.forename: Shijuan
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Cardiology, Haikou People's Hospital, Xiangya School of Medicine Affiliated Haikou Hospital, Central South University, Haikou, 570208, People's Republic of China.
authors.forename: Jianghua
authors.initials: J
authors.lastname: Zhong
authors.affiliation: Department of Cardiology, Haikou People's Hospital, Xiangya School of Medicine Affiliated Haikou Hospital, Central South University, Haikou, 570208, People's Republic of China.
authors.forename: Kang
authors.initials: K
authors.lastname: Huang
authors.affiliation: Department of Cardiology, Xiangya Hospital, Central South University, Changsha, 410078, People's Republic of China. zhangxyxin@sina.com.
authors.forename: Saidan
authors.initials: S
authors.lastname: Zhang
mesh_terms: D000818:Animals; D017209:Apoptosis / Q000187:drug effects; D036341:Intercellular Signaling Peptides and Proteins / Q000378:metabolism*; D009203:Myocardial Infarction / Q000188:drug therapy; D015428:Myocardial Reperfusion Injury / Q000188:drug therapy*; D032383:Myocytes, Cardiac / Q000473:pathology; D018384:Oxidative Stress / Q000187:drug effects; D020011:Protective Agents / Q000494:pharmacology; D011518:Proto-Oncogene Proteins / Q000378:metabolism*; D051381:Rats; D020794:Receptor Protein-Tyrosine Kinases / Q000378:metabolism*; D015398:Signal Transduction / Q000187:drug effects*; D013267:Stilbenes / Q000494:pharmacology* / Q000627:therapeutic use; D000094963:Axl Receptor Tyrosine Kinase; D000099255:Growth Arrest-Specific Protein 6
chemical_list: D036341:Intercellular Signaling Peptides and Proteins; D020011:Protective Agents; D011518:Proto-Oncogene Proteins; D020794:Receptor Protein-Tyrosine Kinases; D013267:Stilbenes; D000094963:Axl Receptor Tyrosine Kinase; D000099255:Growth Arrest-Specific Protein 6; C107773:pterostilbene; C000617974:Axl protein, rat
keywords: Gas6/Axl signaling; inflammation; myocardial ischemia/reperfusion injury; oxidative stress; pterostilbene