title: A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3'-terminal 2'-O-methylated plant microRNA.
abstract: Plant microRNAs (miRNAs) differ from animal miRNAs in that they are 2'-O-methylated at the 3' end nucleotide which limits the ability of plant miRNAs to serve as primers for polymerization reactions. Herein, we have rationally designed a three-way junction structure-based isothermal exponential amplification reaction (3WJ-EXPAR) which is well-suited for sensitive detection of 3'-terminal 2'-O-methylated miRNAs.
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
authors.forename: Xiangdong
authors.initials: X
authors.lastname: Wang
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
authors.forename: Hui
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
authors.forename: Chenghui
authors.identifier: http://orcid.org/0000-0001-8375-0242
authors.initials: C
authors.lastname: Liu
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
authors.forename: Honghong
authors.initials: H
authors.lastname: Wang
authors.affiliation: Key Laboratory of Applied Surface and Colloid Chemistry, Ministry of Education, Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, Xi'an 710062, Shaanxi Province, P. R. China. liuch@snnu.edu.cn lzpbd@snnu.edu.cn.
authors.forename: Zhengping
authors.identifier: http://orcid.org/0000-0002-7573-8822
authors.initials: Z
authors.lastname: Li
mesh_terms: D017360:Arabidopsis / Q000737:chemistry*; D015374:Biosensing Techniques; D035683:MicroRNAs / Q000032:analysis*; D015394:Molecular Structure; D021141:Nucleic Acid Amplification Techniques; D013050:Spectrometry, Fluorescence; D013696:Temperature
chemical_list: D035683:MicroRNAs