title: Antimicrobial capacity of the freshwater planarians against S. aureus is under the control of Timeless.
abstract: Planarians, which are non-parasitic flatworms, are highly resistant to bacterial infections. To better understand the mechanisms underlying this resistance, we investigated the role of the circadian machinery in the anti-bacterial response of the freshwater planarian Schmidtea mediterranea. We identified Smed-Tim from S. mediterranea as a homolog of the mammalian clock gene Tim. We showed via RNA interference that Smed-Tim is required for the anti-microbial activities of Schmidtea mediterranea against Staphylococcus aureus infection during the light/dark cycle. Indeed, S. aureus infection leads to the expression of Smed-Tim, which in turn promotes Smed-Traf6 and Smed-morn2, but not Smed-p38 MAPK expression, 2 master regulators of planarian anti-microbial responses.
authors.affiliation: a CNRS UMR 7278, IRD198, INSERM U1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université , Marseille , France.
authors.forename: Landry Laure
authors.initials: LL
authors.lastname: Tsoumtsa
authors.affiliation: a CNRS UMR 7278, IRD198, INSERM U1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université , Marseille , France.
authors.forename: Cedric
authors.initials: C
authors.lastname: Torre
authors.affiliation: a CNRS UMR 7278, IRD198, INSERM U1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université , Marseille , France.
authors.forename: Virginie
authors.initials: V
authors.lastname: Trouplin
authors.affiliation: a CNRS UMR 7278, IRD198, INSERM U1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université , Marseille , France.|b Department of Respiratory Medicine , Hôpital Nord, Aix-Marseille Université , Marseille , France.
authors.forename: Benjamin
authors.initials: B
authors.lastname: Coiffard
authors.affiliation: c Department of Biochemistry , Otago Genomics & Bioinformatics Facility, University of Otago , Dunedin , New Zealand.
authors.forename: Gregory
authors.initials: G
authors.lastname: Gimenez
authors.affiliation: a CNRS UMR 7278, IRD198, INSERM U1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université , Marseille , France.|d Department of Immunology , APHM, Conception Hospital , Marseille , France.
authors.forename: Jean-Louis
authors.initials: JL
authors.lastname: Mege
authors.affiliation: a CNRS UMR 7278, IRD198, INSERM U1095, Institut Hospitalier Universitaire Méditerranée-Infection, Aix-Marseille Université , Marseille , France.
authors.forename: Eric
authors.initials: E
authors.lastname: Ghigo
mesh_terms: D000818:Animals; D015801:Helminth Proteins / Q000235:genetics / Q000276:immunology*; D008027:Light; D056950:Period Circadian Proteins / Q000235:genetics / Q000276:immunology*; D010932:Planarians / Q000235:genetics / Q000276:immunology* / Q000382:microbiology / Q000528:radiation effects; D013211:Staphylococcus aureus / Q000502:physiology*
chemical_list: D015801:Helminth Proteins; D056950:Period Circadian Proteins
keywords: MORN2; S. aureus; Tim; anti-bacterial response; planarians;