title: Capparis spinosa leaves extract: Source of bioantioxidants with nephroprotective and hepatoprotective effects.
abstract: Capparis spinosa, Capparidaceae, is largely distributed all over the Mediterranean Basin and is traditionally used to treat many illnesses, such as liver and kidney diseases. The aim of the current study was to explore the antioxidant, nephroprotective and hepatoprotective effects of methanolic extract of Capparis spinosa leaves (MECS) associated with its phytochemical content. The levels of total phenolics, flavonoids and condensed tannins were 23.37mgGAE/g, 9.05mgQE/g and 9.35mgTAE/g, respectively. HPLC analysis revealed nine compounds, namely rutin, resveratrol, coumarin, epicatechin, luteolin, catechin, kaempferol, vanillic acid and gallic acid. The MECS showed interesting antioxidant capacity. The MECS-treatment significantly reduced the increased plasma levels of creatinine, urea and uric acid, reduced the elevated MDA levels, significantly reduced the antioxidant enzyme activities and restored the kidney damage, provoked by cisplatin-treatment. Furthermore, MECS-treatment significantly prevented the increase in serum ALT, AST and LDH levels in acute liver damage induced by CCl4, decreased the amount of hepatic malondialdehyde (MDA) formation and elevated the activities of SOD, CAT and GPx, and restored liver injury. This study supports the traditionally use of C. spinosa to cure kidney and liver diseases. The obtained results highlighted the possible use of C. spinosa as a source of phytochemical with important biological advantages.
authors.affiliation: Laboratoire de biochimie, Faculté des Sciences de Tunis, Université Tunis El-Manar, 2092 Tunis, Tunisie; Institut National de Recherches en Génie Rural, Eaux et Forêts, Université de Carthage, BP 10, Ariana 2080, Tunisie. Electronic address: Nizar.Tlili@fst.rnu.tn.
authors.forename: Nizar
authors.initials: N
authors.lastname: Tlili
authors.affiliation: Unité de Biochimie Macromoléculaire et Génétique, Faculté des Sciences de Gafsa, cité Zarroug, Université de Gafsa, 2112 Gafsa, Tunisie; Laboratoire d'Ecophysiologie Animale, Faculté des Sciences de Sfax, Tunisia.
authors.forename: Anouar
authors.initials: A
authors.lastname: Feriani
authors.affiliation: Institut National de Recherches en Génie Rural, Eaux et Forêts, Université de Carthage, BP 10, Ariana 2080, Tunisie.
authors.forename: Ezzeddine
authors.initials: E
authors.lastname: Saadoui
authors.affiliation: Laboratoire de biochimie, Faculté des Sciences de Tunis, Université Tunis El-Manar, 2092 Tunis, Tunisie.
authors.forename: Nizar
authors.initials: N
authors.lastname: Nasri
authors.affiliation: Institut National de Recherches en Génie Rural, Eaux et Forêts, Université de Carthage, BP 10, Ariana 2080, Tunisie.
authors.forename: Abdelhamid
authors.initials: A
authors.lastname: Khaldi
mesh_terms: D000818:Animals; D000975:Antioxidants / Q000302:isolation & purification / Q000494:pharmacology*; D031484:Capparis; D007668:Kidney / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D015227:Lipid Peroxidation / Q000187:drug effects / Q000502:physiology; D008099:Liver / Q000187:drug effects / Q000378:metabolism* / Q000473:pathology; D008297:Male; D010936:Plant Extracts / Q000302:isolation & purification / Q000494:pharmacology*; D018515:Plant Leaves; D020011:Protective Agents / Q000302:isolation & purification / Q000494:pharmacology; D051381:Rats
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D020011:Protective Agents
keywords: Antioxidant; Capparis spinosa; Hepatoprotective; Nephroprotective; Phytochemical