title: Complete genome sequence ofLutibacter profundiLP1Tisolated from an Arctic deep-sea hydrothermal vent system.
abstract: Lutibacter profundiLP1Twithin the familyFlavobacteriaceaewas isolated from a biofilm growing on the surface of a black smoker chimney at the Loki's Castle vent field, located on the Arctic Mid-Ocean Ridge. The complete genome ofL. profundiLP1Tis the first genome to be published within the genusLutibacter. L. profundiLP1Tconsists of a single 2,966,978 bp circular chromosome with a GC content of 29.8%. The genome comprises 2,537 protein-coding genes, 40 tRNA species and 2 rRNA operons. The microaerophilic, organotrophic isolate contains genes for all central carbohydrate metabolic pathways. However, genes for the oxidative branch of the pentose-phosphate-pathway, the glyoxylate shunt of the tricarboxylic acid cycle and the ATP citrate lyase for reverse TCA are not present.L. profundiLP1Tutilizes starch, sucrose and diverse proteinous carbon sources. In accordance, the genome harbours 130 proteases and 104 carbohydrate-active enzymes, indicating a specialization in degrading organic matter. Among a small arsenal of 24 glycosyl hydrolases, which offer the possibility to hydrolyse diverse poly- and oligosaccharides, a starch utilization cluster was identified. Furthermore, a variety of enzymes may be secreted via T9SS and contribute to the hydrolytic variety of the microorganism. Genes for gliding motility are present, which may enable the bacteria to move within the biofilm. A substantial number of genes encoding for extracellular polysaccharide synthesis pathways, curli fibres and attachment to surfaces could mediate adhesion in the biofilm and may contribute to the biofilm formation. In addition to aerobic respiration, the complete denitrification pathway and genes for sulphide oxidation e.g. sulphide:quinone reductase are present in the genome. sulphide:quinone reductase and denitrification may serve as detoxification systems allowingL. profundiLP1Tto thrive in a sulphide and nitrate enriched environment. The information gained from the genome gives a greater insight in the functional role ofL. profundiLP1Tin the biofilm and its adaption strategy in an extreme environment.
authors.affiliation: Centre for Geobiology, University of Bergen, Bergen, Norway ; Department of Biology, University of Bergen, Bergen, Norway.
authors.forename: Juliane
authors.initials: J
authors.lastname: Wissuwa
authors.affiliation: Centre for Geobiology, University of Bergen, Bergen, Norway ; Department of Biology, University of Bergen, Bergen, Norway.
authors.forename: Sven Le Moine
authors.initials: SL
authors.lastname: Bauer
authors.affiliation: Centre for Geobiology, University of Bergen, Bergen, Norway ; Department of Biology, University of Bergen, Bergen, Norway.
authors.forename: Ida Helene
authors.initials: IH
authors.lastname: Steen
authors.affiliation: Centre for Geobiology, University of Bergen, Bergen, Norway ; Department of Biology, University of Bergen, Bergen, Norway.
authors.forename: Runar
authors.identifier: 0000-0002-6056-7119
authors.initials: R
authors.lastname: Stokke
keywords: Biofilm; Deep-sea hydrothermal vent; Flavobacteriaceae; Loki’s castle; Lutibacter