title: Distribution of neurosensory progenitor pools during inner ear morphogenesis unveiled by cell lineage reconstruction.
abstract: Reconstructing the lineage of cells is central to understanding how the wide diversity of cell types develops. Here, we provide the neurosensory lineage reconstruction of a complex sensory organ, the inner ear, by imaging zebrafish embryos in vivo over an extended timespan, combining cell tracing and cell fate marker expression over time. We deliver the first dynamic map of early neuronal and sensory progenitor pools in the whole otic vesicle. It highlights the remodeling of the neuronal progenitor domain upon neuroblast delamination, and reveals that the order and place of neuroblasts' delamination from the otic epithelium prefigure their position within the SAG. Sensory and non-sensory domains harbor different proliferative activity contributing distinctly to the overall growth of the structure. Therefore, the otic vesicle case exemplifies a generic morphogenetic process where spatial and temporal cues regulate cell fate and functional organization of the rudiment of the definitive organ.
authors.affiliation: Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Sylvia
authors.initials: S
authors.lastname: Dyballa
authors.affiliation: Multilevel Dynamics in Morphogenesis Unit, USR3695 CNRS, Gif sur Yvette, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Savy
authors.affiliation: Systems Biology Unit, Center for Genomic Regulation, Barcelona, Spain.
authors.forename: Philipp
authors.identifier: http://orcid.org/0000-0002-2057-4883
authors.initials: P
authors.lastname: Germann
authors.affiliation: Department of Mathematics, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Karol
authors.initials: K
authors.lastname: Mikula
authors.affiliation: Department of Mathematics, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Mariana
authors.initials: M
authors.lastname: Remesikova
authors.affiliation: Department of Mathematics, Slovak University of Technology, Bratislava, Slovakia.
authors.forename: Róbert
authors.initials: R
authors.lastname: Špir
authors.affiliation: Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Andrea
authors.initials: A
authors.lastname: Zecca
authors.affiliation: Multilevel Dynamics in Morphogenesis Unit, USR3695 CNRS, Gif sur Yvette, France.
authors.forename: Nadine
authors.initials: N
authors.lastname: Peyriéras
authors.affiliation: Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Barcelona, Spain.
authors.forename: Cristina
authors.identifier: http://orcid.org/0000-0001-6423-7451
authors.initials: C
authors.lastname: Pujades
mesh_terms: D000818:Animals; D019070:Cell Lineage; D007758:Ear, Inner / Q000166:cytology* / Q000196:embryology*; D009024:Morphogenesis; D061848:Optical Imaging; D011984:Sensory Receptor Cells / Q000502:physiology*; D013234:Stem Cells / Q000502:physiology*; D015027:Zebrafish
keywords: cell biology; cell lineage; developmental biology; inner ear; morphogenesis; neurogenesis; progenitors; sensory development; stem cells; zebrafish