title: Oxidative Stress in Human Aorta of Patients with Advanced Aortoiliac Occlusive Disease.
abstract: Oxidative stress seems to be a role in the atherosclerosis process, but research in human beings is scarce.
  
  
  
  To evaluate the role of oxidative stress on human aortas of patients submitted to surgical treatment for advanced aortoiliac occlusive disease.
  
  
  
  Twenty-six patients were divided into three groups: control group (n=10) formed by cadaveric organ donors; severe aortoiliac stenosis group (patients with severe aortoiliac stenosis; n=9); and total aortoiliac occlusion group (patients with chronic total aortoiliac occlusion; n=7). We evaluated the reactive oxygen species concentration, nicotinamide adenine dinucleotide phosphate-oxidase, superoxide dismutase and catalase activities as well as nitrite levels in samples of aortas harvested during aortofemoral bypass for treatment of advanced aortoiliac occlusive disease.
  
  
  
  We observed a higher level of reactive oxygen species in total aortoiliac occlusion group (48.3±9.56 pmol/mg protein) when compared to severe aortoiliac stenosis (33.5±7.4 pmol/mg protein) and control (4.91±0.8 pmol/mg protein) groups (P<0.05). Nicotinamide adenine dinucleotide phosphate oxidase activity was also higher in total aortoiliac occlusion group when compared to the control group (3.81±1.7 versus 1.05±0.31 µmol/min.mg protein; P<0.05). Furthermore, superoxide dismutase and catalase activities were significantly higher in the severe aortoiliac stenosis and total aortoiliac occlusion groups when compared to the control cases (P<0.05). Nitrite concentration was smaller in the severe aortoiliac stenosis group in comparing to the other groups.
  
  
  
  Our results indicated an increase of reactive oxygen species levels and nicotinamide adenine dinucleotide phosphate-oxidase activity in human aortic samples of patients with advanced aortoiliac occlusive disease. The increase of antioxidant enzymes activities may be due to a compensative phenomenon to reactive oxygen species production mediated by nicotinamide adenine dinucleotide phosphate oxidase. This preliminary study offers us a more comprehensive knowledge about the role of oxidative stress in advanced aortoiliac occlusive disease in human beings.
authors.affiliation: Santa Casa de Porto Alegre, Porto Alegre, RS, Brazil.
authors.forename: Márcio Luís
authors.initials: ML
authors.lastname: Lucas
authors.affiliation: Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Cristina Campos
authors.initials: CC
authors.lastname: Carraro
authors.affiliation: Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
authors.forename: Adriane
authors.initials: A
authors.lastname: Belló-Klein
authors.affiliation: Universidade Federal de Ciências da Saúde (UFCSPA), Porto Alegre, RS, Brazil.
authors.forename: Antonio Nocchi
authors.initials: AN
authors.lastname: Kalil
authors.affiliation: Universidade Federal de Ciências da Saúde (UFCSPA), Porto Alegre, RS, Brazil.
authors.forename: Newton
authors.initials: N
authors.lastname: Aerts
mesh_terms: D000328:Adult; D001018:Aortic Diseases / Q000201:enzymology / Q000601:surgery*; D001157:Arterial Occlusive Diseases / Q000201:enzymology / Q000601:surgery*; D016022:Case-Control Studies; D002374:Catalase / Q000032:analysis; D005260:Female; D006801:Humans; D007083:Iliac Artery / Q000601:surgery*; D008297:Male; D008875:Middle Aged; D009249:NADP / Q000032:analysis; D018384:Oxidative Stress; D012720:Severity of Illness Index; D013482:Superoxide Dismutase / Q000032:analysis; D055815:Young Adult
chemical_list: D009249:NADP; D002374:Catalase; D013482:Superoxide Dismutase