title: Combined thermosensitive in situ gel with AMD3100 in sutureless technique improves the survival and function of kidney transplants in mice.
abstract: The mouse is an optimal animal model for kidney transplantation. Recent reports suggest that application of poloxamer 407, a thermosensitive in situ gel, during the sutureless technique significantly increases animal survival, compared to traditional methods. However, further improvement of this technology is greatly needed but remains unexplored. Here, we detected significant inflammation at the region of ureter anastomosis, after kidney transplantation using poloxamer 407. Since chemokines play a pivotal role during inflammation, we implanted an Alzet osmotic pump that gradually releases AMD3100 (a specific inhibitor of the binding of stromal cell-derived factor 1 (SDF-1) to its receptor, CXCR4) at the site of ureter anastomosis in mice that had undergone kidney transplantation. We found that AMD3100 significantly reduced local inflammation, significantly improved animal survival after kidney transplantation, and significantly improved kidney function. Together, these data suggest that inhibition of chemokine signaling at the site of ureter anastomosis may substantially improve animal survival after kidney transplantation through suppression of suturing-related inflammation.
authors.affiliation: Department of Urology, Shandong University Qilu HospitalJinan 250012, China; Department of Urology, General Hospital of Jinan Military CommandJinan 250031, China.
authors.forename: Nengwang
authors.initials: N
authors.lastname: Yu
authors.affiliation: Shandong Cancer Hospital & Institute, Shandong Academy of Medical Sciences Jinan 250117, China.
authors.forename: Shuai
authors.initials: S
authors.lastname: Fu
authors.affiliation: Department of Urology, General Hospital of Jinan Military Command Jinan 250031, China.
authors.forename: Junwen
authors.initials: J
authors.lastname: Hao
authors.affiliation: Department of Urology, General Hospital of Jinan Military Command Jinan 250031, China.
authors.forename: Aimin
authors.initials: A
authors.lastname: Zhang
authors.affiliation: Department of Orthopedics, General Hospital of Jinan Military Command Jinan 250031, China.
authors.forename: Zhihou
authors.initials: Z
authors.lastname: Fu
keywords: AM3100; CXCR4; Kidney transplantation; inflammation; stromal cell-derived factor 1 (SDF-1); thermosensitive in situ gel poloxamer 407; ureter anastomosis