title: Interaction between Antibacterial Peptide Apep10 and Escherichia coli Membrane Lipids Evaluated Using Liposome as Pseudo-Stationary Phase.
abstract: Liposomes constructed from Escherichia coli membrane lipids were used as a pseudo-stationary phase in capillary electrophoresis and immobilised liposome chromatography to evaluate the interaction between antibacterial peptide (ABP) Apep10 and bacterial membrane lipids. The peptide mobility decreased as the concentration of liposomes increased, providing evidence for the existence of this interaction. The binding constant between Apep10 and the Escherichia coli membranes lipid liposome was higher than that of Apep10 with a mixed phospholipids liposome at the same temperature. The capillary electrophoresis results indicate that the binding ability of Apep10 with a liposome was dependent on the liposome's lipid compositions. Thermodynamic analysis by immobilised liposome chromatography indicated that hydrophobic and electrostatic effects contributed to the partitioning of Apep10 in the membrane lipids. The liposomes constructed from bacterial membrane lipid were more suitable as the model membranes used to study dynamic ABP/membrane interactions than those constructed from specific ratios of particular phospholipids, with its more biomimetic phospholipid composition and contents. This study provides an appropriate model for the evaluation of ABP-membrane interactions.
authors.affiliation: School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Wenting
authors.initials: W
authors.lastname: Tang
authors.affiliation: School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Chuanfen
authors.initials: C
authors.lastname: Pu
authors.affiliation: School of Food Science and Engineering, Qingdao Agricultural University, Qingdao, 266109, China.
authors.forename: Man
authors.initials: M
authors.lastname: Li
mesh_terms: D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002021:Buffers; D002845:Chromatography; D019075:Electrophoresis, Capillary; D004926:Escherichia coli / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry*; D008563:Membrane Lipids / Q000737:chemistry*; D010455:Peptides / Q000494:pharmacology*
chemical_list: D000900:Anti-Bacterial Agents; D002021:Buffers; D008081:Liposomes; D008563:Membrane Lipids; D010455:Peptides