title: Silencing of GP73 inhibits invasion and metastasis via suppression of epithelial-mesenchymal transition in hepatocellular carcinoma.
abstract: Epithelial-mesenchymal transition (EMT) is associated with invasion and metastasis of cancer cells. Golgi protein 73 (GP73) is a serum biomarker for hepatocellular carcinoma (HCC) and our previous study demonstrated that the expression of GP73 correlated with aggressive behavior and EMT molecules in HCC. However, its role in metastatic mechanism of HCC is not clear. The aim of this study was to investigate the effect of GP73 on invasion and migration, and underlying mechanism of GP73 involved in EMT of HCC. The expression of GP73 was downregulated by small interfering RNA (siRNA). The metastatic and invasive abilities were analyzed using scratch assay and Transwell assay. Changes in EMT-related molecules were evaluated by western blot and qRT‑PCR analyses, and epithelial-mesenchymal phenotype changes were also observed. Expression of GP73 was upregulated in the more metastatic HCC cell lines. Knockdown of GP73 by siRNA resulted in a significant decrease in migratory and invasive abilities in both MHCC97H and Bel-7404 cell lines. Importantly, EMT-related markers and morphological phenotypes significantly changed following by the inhibition of GP73. Silencing GP73 contributed to the reduction of invasion and metastasis via suppressing EMT in HCC. GP73 may serve as a novel molecular target against EMT in HCC metastasis therapy.
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Ying
authors.initials: Y
authors.lastname: Yang
authors.affiliation: Department of Urology, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Qiang
authors.initials: Q
authors.lastname: Liu
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Hua
authors.initials: H
authors.lastname: Zhang
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Huarong
authors.initials: H
authors.lastname: Zhao
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Ruin
authors.initials: R
authors.lastname: Mao
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Zhipeng
authors.initials: Z
authors.lastname: Li
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Sha
authors.initials: S
authors.lastname: Ya
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Chunli
authors.initials: C
authors.lastname: Jia
authors.affiliation: Department of Cancer Center, The First Affiliated Hospital of XinJiang Medical University, Urumqi, Xinjiang 830054, P.R. China.
authors.forename: Yongxing
authors.initials: Y
authors.lastname: Bao
mesh_terms: D006528:Carcinoma, Hepatocellular / Q000235:genetics / Q000473:pathology*; D045744:Cell Line, Tumor; D002465:Cell Movement / Q000235:genetics; D058750:Epithelial-Mesenchymal Transition / Q000235:genetics*; D015972:Gene Expression Regulation, Neoplastic; D020868:Gene Silencing; D006801:Humans; D008113:Liver Neoplasms / Q000235:genetics / Q000473:pathology*; D008565:Membrane Proteins / Q000235:genetics*; D034741:RNA, Small Interfering; D014162:Transfection; D015854:Up-Regulation
chemical_list: C410824:GOLM1 protein, human; D008565:Membrane Proteins; D034741:RNA, Small Interfering