title: Saccharomyces cerevisiae Shuttle vectors.
abstract: Yeast shuttle vectors are indispensable tools in yeast research. They enable cloning of defined DNA sequences in Escherichia coli and their direct transfer into Saccharomyces cerevisiae cells. There are three types of commonly used yeast shuttle vectors: centromeric plasmids, episomal plasmids and integrating plasmids. In this review, we discuss the different plasmid systems and their characteristic features. We focus on their segregational stability and copy number and indicate how to modify these properties. Copyright © 2017 John Wiley & Sons, Ltd.
authors.affiliation: D-BSSE, ETH Zurich and Swiss Institute of Bioinformatics, Zurich, Switzerland.|Life Science Zurich PhD Program on Molecular and Translational Biomedicine, Zurich, Switzerland.|Competence Centre for Personalized Medicine, Zurich, Switzerland.
authors.forename: Robert
authors.initials: R
authors.lastname: Gnügge
authors.affiliation: D-BSSE, ETH Zurich and Swiss Institute of Bioinformatics, Zurich, Switzerland.
authors.forename: Fabian
authors.identifier: 0000-0001-5264-333X
authors.initials: F
authors.lastname: Rudolf
mesh_terms: D003001:Cloning, Molecular; D004926:Escherichia coli / Q000235:genetics; D005818:Genetic Engineering / Q000379:methods; D005822:Genetic Vectors / Q000235:genetics; D010957:Plasmids / Q000235:genetics; D012441:Saccharomyces cerevisiae / Q000235:genetics*
keywords: Saccharomyces cerevisiae; copy number; genomic integration; plasmid loss; plasmids; shuttle vectors