title: miR-324-3p suppresses migration and invasion by targeting WNT2B in nasopharyngeal carcinoma.
abstract: BACKGROUND
  Nasopharyngeal carcinoma (NPC) is a malignant epithelial carcinoma of the head and neck with strong ability of invasion and metastasis. Our previous study indicated that miR-324-3p, as a tumor-suppressive factor, could regulate radioresistance of NPC cells by targetingWNT2B. The purpose of this study is to investigate the role of miR-324-3p on migration and invasion in NPC cells.
  
  
  METHODS
  Quantitative real time PCR was applied to measure the expression level of miR-324-3p andWNT2BmRNA in both cells and tissues, and the expression level ofWNT2Bprotein was determined by western blotting. The capacity of migration and invasion were tested by using wound healing and transwell invasion assay.
  
  
  RESULTS
  Ectopic expression of miR-324-3p or silencing its target geneWNT2Bcould dramatically suppress migration and invasion capacity of NPC cells. Meanwhile, the alterations of miR-324-3p in NPC cells could influence the expression level of the biomarkers of epithelial-mesenchymal transition (EMT), including E-cadherin and Vimentin. Moreover, the expression of miR-324-3p was obviously downregulated andWNT2Bwas significantly upregulated in NPC tissues. The expression levels of miR-324-3p andWNT2Bwere closely correlated with T stage, clinic stage and cervical lymph node metastasis of NPC (P< 0.05).
  
  
  CONCLUSIONS
  miR-324-3p could suppress the migration and invasion of NPC by targetingWNT2Band the miR-324-3p/WNT2B pathway possibly provide new potential therapeutic clues for NPC.
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Chao
authors.initials: C
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Guo
authors.initials: G
authors.lastname: Li
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Nianting
authors.initials: N
authors.lastname: Yang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Zhongwu
authors.initials: Z
authors.lastname: Su
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Shuiting
authors.initials: S
authors.lastname: Zhang
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Tengbo
authors.initials: T
authors.lastname: Deng
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Shuling
authors.initials: S
authors.lastname: Ren
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Shanhong
authors.initials: S
authors.lastname: Lu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Yongquan
authors.initials: Y
authors.lastname: Tian
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Yong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: Department of Otolaryngology Head and Neck Surgery, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, 410008 Hunan China.|Otolaryngology Major Disease Research Key Laboratory of Hunan Province, Changsha, 410008 Hunan China.
authors.forename: Yuanzheng
authors.initials: Y
authors.lastname: Qiu
keywords: Invasion; Nasopharyngeal carcinoma; WNT2B; miR-324-3p