title: Electroporation in the Rodent Embryonic Brain Using Whole Embryo Culture System.
abstract: This unit describes basic methods for mammalian whole embryo culture (WEC) using embryonic day 10.5 mouse embryos, including the preparation of high-quality immediately centrifuged (IC) rat serum that is commonly used for WEC and is essential for normal growth and development of cultured mouse and rat embryos in vitro. An alternative protocol for different stages of rodent embryos is also introduced. Since embryos for WEC are dissected out of the uterus and manipulated under the microscope, one can overcome many of the difficulties of gene delivery encountered using in utero electroporation. A description for a gene transfer method to label neural stem/progenitor cells of the cortical primordium in a highly region-specific manner is also included. © 2017 by John Wiley & Sons, Inc.
authors.affiliation: Department of Developmental Neuroscience, United Center for Advanced Research and Translational Medicine (ART), Tohoku University School of Medicine, Sendai, Miyagi, Japan.
authors.forename: Takako
authors.initials: T
authors.lastname: Kikkawa
authors.affiliation: Jichi Medical University Graduate School of Medicine, Shimotsuke, Tochigi, Japan.|Division of Biology, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.
authors.forename: Masanori
authors.initials: M
authors.lastname: Takahashi
authors.affiliation: Department of Developmental Neuroscience, United Center for Advanced Research and Translational Medicine (ART), Tohoku University School of Medicine, Sendai, Miyagi, Japan.
authors.forename: Noriko
authors.initials: N
authors.lastname: Osumi
mesh_terms: D000818:Animals; D001921:Brain / Q000378:metabolism; D018274:Electroporation / Q000379:methods*; D046149:Embryo Culture Techniques / Q000379:methods; D004622:Embryo, Mammalian / Q000378:metabolism*; D047108:Embryonic Development / Q000502:physiology; D018014:Gene Transfer Techniques; D051379:Mice; D051381:Rats; D012377:Rodentia
keywords: brain development; electroporation; whole embryo culture