title: Combined microplate-ABTS and HPLC-ABTS analysis of tomato and pepper extracts reveals synergetic and antagonist effects of their lipophilic antioxidative components.
abstract: The antioxidant capacity of 9 pure lipophilic compounds was examined by microplate-ABTS and HPLC-ABTS, using similar experimental conditions. Results obtained showed that HPLC-ABTS method can be used for a rapid determination of individual antioxidant capacity of compounds in standard solutions or complex mixtures. The application of both methods to real lipophilic extracts from tomato (Solanum lycopersicum L.), green and red peppers (Capsicum annuum) reveals possible interactions between antioxidants. Thus, synthetic mixtures of two compounds identified in tomato and peppers were measured using microplate-ABTS and HPLC-ABTS. Synergistic effects were observed between (β-carotene-capsanthin) (1:9) and (1:1), (α-tocopherol-capsanthin) (1:9), (lutein-lycopene) (9:1) and (capsanthin-δ-tocopherol) (9:1). On the contrary, antagonistic effects were observed for (lutein-δ-tocopherol) and (α-tocopherol-δ-tocopherol). The interactions observed with two-compound mixtures are not systematically observed in the natural lipophilic extracts from tomato, green and red peppers, probably since extracts are more complex and are susceptible to cause interferences.
authors.affiliation: Aérial, ITAI-CRT, 250 rue Laurent Fries, F-67400 Illkirch-Graffenstaden, France. Electronic address: jlg@lesaffre.fr.
authors.forename: Julie
authors.initials: J
authors.lastname: Le Grandois
authors.affiliation: Aérial, ITAI-CRT, 250 rue Laurent Fries, F-67400 Illkirch-Graffenstaden, France. Electronic address: delphine.guffond@gmail.com.
authors.forename: Delphine
authors.initials: D
authors.lastname: Guffond
authors.affiliation: Aérial, ITAI-CRT, 250 rue Laurent Fries, F-67400 Illkirch-Graffenstaden, France. Electronic address: e.hamon@aerial-crt.com.
authors.forename: Erwann
authors.initials: E
authors.lastname: Hamon
authors.affiliation: CAMBA, Université de Strasbourg, IPHC, 74, route du Rhin, F-67400 Illkirch-Graffenstaden, France. Electronic address: eric.marchioni@unistra.fr.
authors.forename: Eric
authors.initials: E
authors.lastname: Marchioni
authors.affiliation: Aérial, ITAI-CRT, 250 rue Laurent Fries, F-67400 Illkirch-Graffenstaden, France. Electronic address: da.werner@aerial-crt.com.
authors.forename: Dalal
authors.initials: D
authors.lastname: Werner
mesh_terms: D000975:Antioxidants / Q000032:analysis* / Q000378:metabolism; D052160:Benzothiazoles / Q000737:chemistry*; D002212:Capsicum / Q000737:chemistry / Q000378:metabolism; D002338:Carotenoids / Q000032:analysis / Q000378:metabolism; D002851:Chromatography, High Pressure Liquid / Q000379:methods; D004357:Drug Synergism; D014975:Lutein / Q000032:analysis / Q000378:metabolism; D018551:Solanum lycopersicum / Q000737:chemistry / Q000378:metabolism; D010936:Plant Extracts / Q000032:analysis* / Q000378:metabolism; D013451:Sulfonic Acids / Q000737:chemistry*; D024341:Xanthophylls / Q000032:analysis / Q000378:metabolism; D024502:alpha-Tocopherol / Q000032:analysis / Q000378:metabolism; D019207:beta Carotene / Q000032:analysis / Q000378:metabolism
chemical_list: D000975:Antioxidants; D052160:Benzothiazoles; D010936:Plant Extracts; D013451:Sulfonic Acids; D024341:Xanthophylls; D019207:beta Carotene; C002502:2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid; D002338:Carotenoids; C006889:capsanthin; D024502:alpha-Tocopherol; D014975:Lutein
keywords: Antagonisms; Antioxidant capacity; HPLC-ABTS; Lipophilic compounds; Synergisms