title: A crosslinker-based identification of redox relay targets.
abstract: Thiol-based redox control is among the most important mechanisms for maintaining cellular redox homeostasis, with essential participation of cysteine thiols of oxidoreductases. To explore cellular redox regulatory networks, direct interactions among active cysteine thiols of oxidoreductases and their targets must be clarified. We applied a recently described thiol-ene crosslinking-based strategy, named divinyl sulfone (DVSF) method, enabling identification of new potential redox relay partners of the cytosolic oxidoreductases thioredoxin (TXN) and thioredoxin domain containing 17 (TXNDC17). Applying multiple methods, including classical substrate-trapping techniques, will increase understanding of redox regulatory mechanisms in cells.
authors.affiliation: Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan. Electronic address: k-araki@aist.go.jp.
authors.forename: Kazutaka
authors.initials: K
authors.lastname: Araki
authors.affiliation: Faculty of Life Sciences, Kyoto Sangyo University, Kita-Ku, Kyoto 603-8555, Japan.
authors.forename: Ryo
authors.initials: R
authors.lastname: Ushioda
authors.affiliation: Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan.
authors.forename: Hidewo
authors.initials: H
authors.lastname: Kusano
authors.affiliation: Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan.
authors.forename: Riko
authors.initials: R
authors.lastname: Tanaka
authors.affiliation: Robotic Biology Institute, Inc., Tokyo 135-0064, Japan.
authors.forename: Tomohisa
authors.initials: T
authors.lastname: Hatta
authors.affiliation: Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan.
authors.forename: Kazuhiko
authors.initials: K
authors.lastname: Fukui
authors.affiliation: Faculty of Life Sciences, Kyoto Sangyo University, Kita-Ku, Kyoto 603-8555, Japan.
authors.forename: Kazuhiro
authors.initials: K
authors.lastname: Nagata
authors.affiliation: Molecular Profiling Research Center for Drug Discovery, National Institute of Advanced Industrial Science and Technology, Tokyo 135-0064, Japan; Robotic Biology Institute, Inc., Tokyo 135-0064, Japan.
authors.forename: Tohru
authors.initials: T
authors.lastname: Natsume
mesh_terms: D000595:Amino Acid Sequence; D003432:Cross-Linking Reagents / Q000737:chemistry*; D057809:HEK293 Cells; D006801:Humans; D008969:Molecular Sequence Data; D010084:Oxidation-Reduction; D016415:Sequence Alignment; D013450:Sulfones / Q000737:chemistry; D013879:Thioredoxins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism*
chemical_list: D003432:Cross-Linking Reagents; D013450:Sulfones; C482341:TXNDC17 protein, human; D013879:Thioredoxins; C009873:divinyl sulfone
keywords: Interaction; Oxidoreductase; Proteomics; Redox; Thioredoxin