title: Genome-Wide DNA Methylation Patterns Analysis of Noncoding RNAs in Temporal Lobe Epilepsy Patients.
abstract: Temporal lobe epilepsy (TLE) is the most common form of adult epilepsy and frequently evolving drug resistance. Although there is growing consensus that noncoding ribonucleic acids (ncRNAs) are modulators of TLE, the knowledge about the deoxyribonucleic acid (DNA) methylation patterns of ncRNAs in TLE remains limited. In the current study, we constructed DNA methylation profiles from 30 TLE patients and 30 healthy controls for ncRNAs, primarily focusing on long ncRNAs (lncRNAs) and microRNAs (miRNAs), by reannotating data of DNA methylation BeadChip. Statistics analyses have revealed a global hypermethylation pattern in miRNA and lncRNA gene in TLE patients. Bioinformatic analyses have found aberrantly methylated miRNAs and lncRNAs are related to ion channel activity, drug metabolism, mitogen-activated protein kinase (MAPK) signaling pathway, and neurotrophin signaling pathway. Aberrantly methylated ncRNA and pathway target might be involved in TLE development and progression. The methylated and demethylated ncRNAs identified in this study provide novel insights for developing TLE biomarkers and potential therapeutic targets.
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Wenbiao
authors.initials: W
authors.lastname: Xiao
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, China.|Department of Neurology, Peking Union Medical College Hospital, Beijing, 100032, China.
authors.forename: Yuze
authors.initials: Y
authors.lastname: Cao
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Hongyu
authors.initials: H
authors.lastname: Long
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Zhaohui
authors.initials: Z
authors.lastname: Luo
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Shuyu
authors.initials: S
authors.lastname: Li
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China.
authors.forename: Na
authors.initials: N
authors.lastname: Deng
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, China.
authors.forename: Jianjian
authors.initials: J
authors.lastname: Wang
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Lu
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, China.
authors.forename: Tianfeng
authors.initials: T
authors.lastname: Wang
authors.affiliation: College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, 150081, China. ningsw@ems.hrbmu.edu.cn.
authors.forename: Shangwei
authors.initials: S
authors.lastname: Ning
authors.affiliation: Department of Neurology, The Second Affiliated Hospital, Harbin Medical University, Harbin, 150081, China. wanglh211@163.com.
authors.forename: Lihua
authors.initials: L
authors.lastname: Wang
authors.affiliation: Department of Neurology, Xiangya Hospital, Central South University, Changsha, 410008, China. mdboxiao@163.com.
authors.forename: Bo
authors.initials: B
authors.lastname: Xiao
mesh_terms: D000328:Adult; D016000:Cluster Analysis; D019175:DNA Methylation / Q000235:genetics*; D004833:Epilepsy, Temporal Lobe / Q000235:genetics*; D005260:Female; D015894:Genome, Human; D006801:Humans; D008297:Male; D035683:MicroRNAs / Q000235:genetics / Q000378:metabolism; D058977:Molecular Sequence Annotation; D022661:RNA, Untranslated / Q000235:genetics* / Q000378:metabolism
chemical_list: D035683:MicroRNAs; D022661:RNA, Untranslated
keywords: DNA methylation; Epigenetic; Long noncoding RNA (lncRNA); MicroRNA (miRNA); Temporal lobe epilepsy (TLE)