title: Stochastic models of gene transcription with upstream drives: exact solution and sample path characterization.
abstract: Gene transcription is a highly stochastic and dynamic process. As a result, the mRNA copy number of a given gene is heterogeneous both between cells and across time. We present a framework to model gene transcription in populations of cells with time-varying (stochastic or deterministic) transcription and degradation rates. Such rates can be understood as upstream cellular drives representing the effect of different aspects of the cellular environment. We show that the full solution of the master equation contains two components: a model-specific, upstream effective drive, which encapsulates the effect of cellular drives (e.g. entrainment, periodicity or promoter randomness) and a downstream transcriptional Poissonian part, which is common to all models. Our analytical framework treats cell-to-cell and dynamic variability consistently, unifying several approaches in the literature. We apply the obtained solution to characterize different models of experimental relevance, and to explain the influence on gene transcription of synchrony, stationarity, ergodicity, as well as the effect of time scales and other dynamic characteristics of drives. We also show how the solution can be applied to the analysis of noise sources in single-cell data, and to reduce the computational cost of stochastic simulations.
authors.affiliation: Department of Mathematics, Imperial College London, London SW7 2AZ, UK.
authors.forename: Justine
authors.identifier: 0000-0002-5908-278X
authors.initials: J
authors.lastname: Dattani
authors.affiliation: Department of Mathematics, Imperial College London, London SW7 2AZ, UK m.barahona@imperial.ac.uk.
authors.forename: Mauricio
authors.identifier: 0000-0002-1089-5675
authors.initials: M
authors.lastname: Barahona
mesh_terms: D005786:Gene Expression Regulation / Q000502:physiology*; D008957:Models, Genetic; D013269:Stochastic Processes; D014158:Transcription, Genetic / Q000502:physiology*
keywords: chemical master equation; gene expression; noise; non-stationarity; stochastic models; transcription