title: Salecan-Based pH-Sensitive Hydrogels for Insulin Delivery.
abstract: Stimuli-responsive polymeric hydrogels are promising and appealing delivery vehicles for protein/peptide drugs and have made protein/peptide delivery with both dosage- and spatiotemporal-controlled manners possible. Here a series of new Salecan-based pH-sensitive hydrogels were fabricated for controlled insulin delivery via the graft copolymerization reaction between Salecan and 2-acrylamido-2-methyl-1-propanesulfonic acid. In this study, on one hand, Salecan played a key role in modifying the structure and the pore size of the developing hydrogel. On the other hand, Salecan tuned the water content and the water release rate of the obtained hydrogel, leading to a controllable release rate of the insulin. More importantly, in vitro release experiments validated that the release of insulin from this intelligent system could be also tailored by the environmental pH of the release medium. For SGA2, the amount of encapsulated insulin released at gastric conditions (pH 1.2) was relatively low (about 26.1 wt % in 24 h), while that released at intestinal conditions (pH 7.4) increased significantly (over 50 wt % in 6 h). Furthermore, toxicity assays demonstrated that the designed hydrogel carriers were biocompatible. These characteristics make the Salecan-based hydrogel a promising candidate for protein/peptide drug delivery device.
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Xiaoliang
authors.initials: X
authors.lastname: Qi
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Wei
authors.initials: W
authors.lastname: Wei
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Junjian
authors.initials: J
authors.lastname: Li
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Gancheng
authors.initials: G
authors.lastname: Zuo
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Xihao
authors.initials: X
authors.lastname: Pan
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Ting
authors.initials: T
authors.lastname: Su
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Jianfa
authors.initials: J
authors.lastname: Zhang
authors.affiliation: Center for Molecular Metabolism, Nanjing University of Science & Technology , Nanjing 210094, China.
authors.forename: Wei
authors.identifier: 0000-0002-6712-8960
authors.initials: W
authors.lastname: Dong
mesh_terms: D000178:Acrylamides / Q000737:chemistry; D000476:Alkanesulfonates / Q000737:chemistry; D000818:Animals; D019556:COS Cells; D002460:Cell Line; D004337:Drug Carriers / Q000737:chemistry; D016503:Drug Delivery Systems / Q000379:methods; D005079:Excipients / Q000737:chemistry; D057809:HEK293 Cells; D006801:Humans; D020100:Hydrogels / Q000737:chemistry*; D006863:Hydrogen-Ion Concentration; D007328:Insulin / Q000737:chemistry*; D011108:Polymers / Q000737:chemistry; D047071:beta-Glucans / Q000737:chemistry*
chemical_list: D000178:Acrylamides; D000476:Alkanesulfonates; D004337:Drug Carriers; D005079:Excipients; D020100:Hydrogels; D007328:Insulin; D011108:Polymers; D047071:beta-Glucans; C577623:salecan; C056288:2-acrylamido-2-methylpropanesulfonate
keywords: 2-acrylamido-2-methyl-1-propanesulfonic acid; drug delivery; insulin; pH-sensitive hydrogels; salecan