title: First Molecular Identifications of Necator americanus and Ancylostoma ceylanicum Infecting Rural Communities in Lower Myanmar.
abstract: Hookworms are enteric parasitic roundworms infecting an estimated 400 million persons worldwide. Herein, we provide the first molecular identifications of human hookworms from certain parts of rural Lower Myanmar. DNA was extracted from hookworm-positive stool samples, as determined by microscopy. DNA sequences of the partial internal transcribed spacer 1, full length 5.8S gene, and partial internal transcribed spacer 2 were determined and compared with available hookworm sequences from public databases. Of the 11 polymerase chain reaction-positive samples, eight (Bago Region, N = 4; Mon State, N = 4) yielded sequences with high similarity to those of Necator americanus A further three sequences (Mon State, N = 2; Bago Region, N = 1) showed high similarity with those of Ancylostoma ceylanicum The latter is primarily a parasite of dogs and represents a zoonosis. Given that different species of hookworms exhibit different epidemiological and biological characteristics, accurate identification is essential for the planning and execution of effective control programs for hookworm infections.
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.|Department of Microbiology, University of Medicine 2, Ministry of Health and Sport, Yangon, Myanmar.
authors.forename: Win
authors.initials: W
authors.lastname: Pa Pa Aung
authors.affiliation: Department of Parasitology, National Health Laboratory, Yangon, Myanmar.
authors.forename: Thi Thi
authors.initials: TT
authors.lastname: Htoon
authors.affiliation: Department of Parasitology, National Health Laboratory, Yangon, Myanmar.
authors.forename: Htay Htay
authors.initials: HH
authors.lastname: Tin
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Oranuch
authors.initials: O
authors.lastname: Sanpool
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Jurairat
authors.initials: J
authors.lastname: Jongthawin
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Lakkhana
authors.initials: L
authors.lastname: Sadaow
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Issarapong
authors.initials: I
authors.lastname: Phosuk
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Rutchanee
authors.initials: R
authors.lastname: Ropai
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
authors.forename: Pewpan M
authors.initials: PM
authors.lastname: Intapan
authors.affiliation: Department of Parasitology and Research and Diagnostic Center for Emerging Infectious Disease, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. wanch_ma@kku.ac.th.
authors.forename: Wanchai
authors.initials: W
authors.lastname: Maleewong
mesh_terms: D000722:Ancylostoma / Q000302:isolation & purification*; D000818:Animals; D018509:DNA, Helminth / Q000235:genetics / Q000302:isolation & purification; D005243:Feces / Q000469:parasitology; D006801:Humans; D002052:Myanmar / Q000453:epidemiology; D017221:Necator americanus / Q000302:isolation & purification*; D009332:Necatoriasis / Q000453:epidemiology* / Q000469:parasitology*; D016133:Polymerase Chain Reaction; D012424:Rural Population; D013045:Species Specificity; D015047:Zoonoses
chemical_list: D018509:DNA, Helminth