title: Anti-MUC1 nano-aptamers for triple-negative breast cancer imaging by single-photon emission computed tomography in inducted animals: initial considerations.
abstract: The early and specific detection of tumors remains a barrier in oncology, especially in cases such as the triple-negative breast cancer (TNBC). To address this gap, aptamers have found an important application in the recognition of tumor biomarkers such as mucin 1 (MUC1). However, there are still some difficulties in the use of aptamer, as their rapid biological clearance makes their use as drugs limited. In this study, the anti-MUC1 aptamer was used as a drug delivery system (DDS) for a radioactive polymeric nanoparticle (NP) in the imaging of TNBCs. Thus, poly(lactic-co-glycolic acid) NPs loaded with the anti-MUC1 aptamer and labeled with technetium-99m were used for a biodistribution study and imaging of TNBC. The results confirmed that the NP was successfully obtained, with a mean size of 262 nm, according to the dynamic light scattering data. The biodistribution assay in induced animal models with TNBC showed that although there was a high capture by intestine (>30%), the DDS developed had a high tumor uptake (5%) and with great in vivo imaging properties, corroborating the possibility of use of this DDS as an imaging drug for TNBC.
authors.affiliation: Rio de Janeiro State University, Biology Institute Roberto Alcantara Gomes; Brazilian Nuclear Energy Commission, Nuclear Engineering Institute.
authors.forename: Fagner
authors.initials: F
authors.lastname: Santos do Carmo
authors.affiliation: Federal University of Rio de Janeiro, Faculty of Pharmacy.
authors.forename: Eduardo
authors.initials: E
authors.lastname: Ricci-Junior
authors.affiliation: Federal University of Rio de Janeiro, Institute of Macromolecules Eloisa Mano.
authors.forename: Cristal
authors.initials: C
authors.lastname: Cerqueira-Coutinho
authors.affiliation: Rio de Janeiro State University, Biology Institute Roberto Alcantara Gomes; University Hospital Clementino Fraga Filho, Federal University of Rio de Janeiro, Rio de Janeiro.
authors.forename: Marta de Souza
authors.initials: MS
authors.lastname: Albernaz
authors.affiliation: Instituto de Pesquisas Energéticas e Nucleares, Centro de Radiofarmácia, São Paulo.
authors.forename: Emerson Soares
authors.initials: ES
authors.lastname: Bernardes
authors.affiliation: Institute of Technology in Immunobiologics Bio-Manguinhos, Oswaldo Cruz Foundation (Fiocruz), Rio de Janeiro, Brazil.
authors.forename: Sotiris
authors.initials: S
authors.lastname: Missailidis
authors.affiliation: Brazilian Nuclear Energy Commission, Nuclear Engineering Institute.
authors.forename: Ralph
authors.initials: R
authors.lastname: Santos-Oliveira
mesh_terms: D000818:Animals; D052158:Aptamers, Peptide / Q000737:chemistry*; D045744:Cell Line, Tumor; D016503:Drug Delivery Systems / Q000379:methods; D000067493:Dynamic Light Scattering; D005260:Female; D006801:Humans; D008807:Mice, Inbred BALB C; D018396:Mucin-1 / Q000378:metabolism*; D053758:Nanoparticles / Q000737:chemistry; D011108:Polymers / Q000737:chemistry; D019275:Radiopharmaceuticals / Q000737:chemistry / Q000493:pharmacokinetics; D013667:Technetium / Q000737:chemistry; D014018:Tissue Distribution; D015899:Tomography, Emission-Computed, Single-Photon / Q000379:methods*; D064726:Triple Negative Breast Neoplasms / Q000000981:diagnostic imaging* / Q000378:metabolism; D023041:Xenograft Model Antitumor Assays
chemical_list: D052158:Aptamers, Peptide; C060192:MUC1 protein, human; D018396:Mucin-1; D011108:Polymers; D019275:Radiopharmaceuticals; D013667:Technetium
keywords: aptamer; cancer control; imaging; nuclear medicine; radiopharmacy