title: Liquid Chromatography-High Resolution Mass Spectrometry Method to Study Sphingolipid Metabolism Changes in Response to CD95L.
abstract: Sphingolipids are sphingoid base-containing lipids, among which some metabolites behave as bioactive molecules in various biological processes, including cell death. Whereas ceramide is now viewed as an anti-oncometabolite, leading to cancer cell death, CD95L-induced apoptosis is associated with sphingolipid changes, which likely contribute to caspase-dependent signaling pathway activation. Here, we describe Liquid Chromatography-high resolution mass spectrometry method (LC-HRMS) to analyze sphingolipid metabolism changes triggered by CD95L.
authors.affiliation: INSERM UMR 1037, CRCT, 31037, Toulouse, France.|Equipe Labellisée Ligue Contre Le Cancer, 31037, Toulouse, France.|Ecole Doctorale de Sciences et Technologies, Université Libanaise, Campus Universitaire de Rafic Hariri, Hadath, Lebanon.
authors.forename: Fatima
authors.initials: F
authors.lastname: Bilal
authors.affiliation: INSERM UMR 1037, CRCT, 31037, Toulouse, France.|Equipe Labellisée Ligue Contre Le Cancer, 31037, Toulouse, France.|Université Toulouse III-Paul Sabatier, 118 route de Narbonne, 31062, Toulouse, France.
authors.forename: Michaël
authors.initials: M
authors.lastname: Pérès
authors.affiliation: INSERM UMR 1048, Plateau MetaToul-Lipidomique, MetaboHUB, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), 31432, Toulouse Cedex 4, France.
authors.forename: Pauline
authors.initials: P
authors.lastname: Le Faouder
authors.affiliation: INSERM UMR 1048, Plateau MetaToul-Lipidomique, MetaboHUB, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), 31432, Toulouse Cedex 4, France.
authors.forename: Aude
authors.initials: A
authors.lastname: Dupuy
authors.affiliation: INSERM UMR 1048, Plateau MetaToul-Lipidomique, MetaboHUB, Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), 31432, Toulouse Cedex 4, France.
authors.forename: Justine
authors.initials: J
authors.lastname: Bertrand-Michel
authors.affiliation: INSERM UMR 1037, CRCT, 31037, Toulouse, France.|Equipe Labellisée Ligue Contre Le Cancer, 31037, Toulouse, France.
authors.forename: Nathalie
authors.initials: N
authors.lastname: Andrieu-Abadie
authors.affiliation: INSERM UMR 1037, CRCT, 31037, Toulouse, France.|Equipe Labellisée Ligue Contre Le Cancer, 31037, Toulouse, France.|Université Toulouse III-Paul Sabatier, 118 route de Narbonne, 31062, Toulouse, France.|Laboratoire de Biochimie, Institut Fédératif de Biologie, CHU Purpan, 31059, Toulouse, France.
authors.forename: Thierry
authors.initials: T
authors.lastname: Levade
authors.affiliation: Ecole Doctorale de Sciences et Technologies, Université Libanaise, Campus Universitaire de Rafic Hariri, Hadath, Lebanon.
authors.forename: Bassam
authors.initials: B
authors.lastname: Badran
authors.affiliation: Ecole Doctorale de Sciences et Technologies, Université Libanaise, Campus Universitaire de Rafic Hariri, Hadath, Lebanon.
authors.forename: Ahmad
authors.initials: A
authors.lastname: Daher
authors.affiliation: INSERM UMR 1037, CRCT, 31037, Toulouse, France. bruno.segui@inserm.fr.|Equipe Labellisée Ligue Contre Le Cancer, 31037, Toulouse, France. bruno.segui@inserm.fr.|Université Toulouse III-Paul Sabatier, 118 route de Narbonne, 31062, Toulouse, France. bruno.segui@inserm.fr.
authors.forename: Bruno
authors.initials: B
authors.lastname: Ségui
mesh_terms: D017209:Apoptosis; D002518:Ceramides / Q000378:metabolism; D002853:Chromatography, Liquid; D053222:Fas Ligand Protein / Q000378:metabolism*; D050356:Lipid Metabolism; D013058:Mass Spectrometry; D011485:Protein Binding; D013107:Sphingolipids / Q000378:metabolism*
chemical_list: D002518:Ceramides; D053222:Fas Ligand Protein; D013107:Sphingolipids
keywords: Apoptosis; Ceramide; Liquid Chromatography–high resolution mass spectrometry; Sphingomyelin