title: Conductive hydrogel composed of 1,3,5-benzenetricarboxylic acid and Fe3+used as enhanced electrochemical immunosensing substrate for tumor biomarker.
abstract: In this work, a new conductive hydrogel was prepared by a simple cross-linking coordination method using 1,3,5-benzenetricarboxylic acid as the ligand and Fe3+as the metal ion. The hydrogel film was formed on a glassy carbon electrode (GCE) by a drop coating method, which can dramatically facilitate the transport of electrons. A sensitive label-free electrochemical immunosensor was fabricated following electrodeposition of gold nanoparticles (AuNPs) on a hydrogel film and immobilization of an antibody. Neuron-specific enolase (NSE), a lung cancer biomarker, was used as the model analyte to be detected. The proposed immunosensor exhibited a wide linear detection range of 1pgmL-1to 200ngmL-1and a limit of detection of 0.26pgmL-1(the ratio of signal to noise (S/N)=3). Moreover, the detection of NSE in human serum samples showed satisfactory accuracy compared with the data determined by enzyme-linked immunosorbent assay (ELISA), indicating good analytical performance of the immunoassay.
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Huiqiang
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China.
authors.forename: Hongliang
authors.initials: H
authors.lastname: Han
authors.affiliation: Department of Chemistry, Capital Normal University, Beijing 100048, China. Electronic address: mazhanfang@cnu.edu.cn.
authors.forename: Zhanfang
authors.initials: Z
authors.lastname: Ma
mesh_terms: D014408:Biomarkers, Tumor / Q000032:analysis* / Q000737:chemistry; D015374:Biosensing Techniques / Q000295:instrumentation / Q000379:methods*; D004553:Electric Conductivity; D004563:Electrochemistry; D006046:Gold / Q000737:chemistry; D006801:Humans; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate / Q000737:chemistry*; D007118:Immunoassay / Q000295:instrumentation / Q000379:methods*; D057230:Limit of Detection; D053768:Metal Nanoparticles / Q000737:chemistry; D010751:Phosphopyruvate Hydratase / Q000032:analysis / Q000737:chemistry; D014233:Tricarboxylic Acids / Q000737:chemistry*
chemical_list: D014408:Biomarkers, Tumor; D014233:Tricarboxylic Acids; D020136:Hydrogel, Polyethylene Glycol Dimethacrylate; D006046:Gold; D010751:Phosphopyruvate Hydratase; C069849:trimesic acid
keywords: 1,3,5-Benzenetricarboxylic acid; Conductive hydrogel; Electrochemical immunosensor; Iron ion; Neuron-specific enolase; Tumor biomarker