title: Standardization, Calibration, and Control in Flow Cytometry.
abstract: Because flow cytometers are designed to measure particle characteristics, particles are the most common materials used to calibrate, control, and standardize the instruments. Definitions and cautions are provided for common terms to alert the reader to critical distinctions in meaning. This unit presents extensive background on particle types and cautions and describes practical aspects of methods to standardize and calibrate instruments. Procedures are provided to characterize performance in terms of optical alignment, fluorescence and light scatter resolution, and sensitivity. Finally, suggestions follow for analyzing particles used for calibration. © 2017 by John Wiley & Sons, Inc.
authors.affiliation: Biosystems and Biomaterials Division, NIST, Gaithersburg, Maryland.
authors.forename: Lili
authors.initials: L
authors.lastname: Wang
authors.affiliation: Independent Consultant, Livermore, California.
authors.forename: Robert A
authors.initials: RA
authors.lastname: Hoffman
mesh_terms: D002138:Calibration; D005434:Flow Cytometry / Q000295:instrumentation / Q000379:methods* / Q000592:standards*; D012015:Reference Standards
keywords: ERF (equivalent number of reference fluorophores); MESF (molecules of equivalent soluble fluorochrome); calibration; control; particles; resolution; sensitivity; standardization