title: Porcine mast cells infected with H1N1 influenza virus release histamine and inflammatory cytokines and chemokines.
abstract: Mast cells reside in many tissues, including the lungs, and might play a role in enhancing influenza virus infections in animals. In this study, we cultured porcine mast cells from porcine bone marrow cells with IL-3 and stem cell factor to study the infectivity and activation of the 2009 pandemic H1N1 influenza virus of swine origin. Porcine mast cells were infected with H1N1 influenza virus, without the subsequent production of infectious viruses but were activated, as indicated by the release of histamines. Inflammatory cytokine- and chemokine-encoding genes, including IL-1α, IL-6, CXCL9, CXCL10, and CXCL11, were upregulated in the infected porcine mast cells. Our results suggest that mast cells could be involved in enhancing influenza-virus-mediated disease in infected animals.
authors.affiliation: Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University, 99 Dae-Hak Ro, Yuseong Gu, Daejeon, 305-764, Republic of Korea.|Institute of Influenza Virus, College of Veterinary Medicine, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: In Hong
authors.initials: IH
authors.lastname: Lee
authors.affiliation: Laboratory of Public Health, College of Veterinary Medicine, Chungnam National University, Daejeon, 305-764, Republic of Korea.
authors.forename: Hyun Soo
authors.initials: HS
authors.lastname: Kim
authors.affiliation: Laboratory of Influenza Research, College of Veterinary Medicine, Chungnam National University, 99 Dae-Hak Ro, Yuseong Gu, Daejeon, 305-764, Republic of Korea. seos@cnu.ac.kr.|Institute of Influenza Virus, College of Veterinary Medicine, Chungnam National University, Daejeon, 305-764, Republic of Korea. seos@cnu.ac.kr.
authors.forename: Sang Heui
authors.initials: SH
authors.lastname: Seo
mesh_terms: D000818:Animals; D002460:Cell Line; D018925:Chemokines / Q000378:metabolism*; D016207:Cytokines / Q000378:metabolism*; D006632:Histamine / Q000378:metabolism*; D053118:Influenza A Virus, H1N1 Subtype / Q000235:genetics / Q000502:physiology*; D008407:Mast Cells / Q000378:metabolism*; D009976:Orthomyxoviridae Infections / Q000378:metabolism* / Q000821:virology; D013552:Swine; D013553:Swine Diseases / Q000378:metabolism* / Q000821:virology
chemical_list: D018925:Chemokines; D016207:Cytokines; D006632:Histamine
keywords: Inflammation; Influenza virus; Mast cells