title: Transcriptomic Profiling Reveals Metabolic and Regulatory Pathways in the Desiccation Tolerance of Mungbean (Vigna radiata[L.] R. Wilczek).
abstract: Mungbean (Vigna radiateL. Wilczek) is an important legume crop for its valuable nutritional and health benefits. Desiccation tolerance (DT) is a capacity of seeds to survive and maintain physiological activities during storage and under stress conditions. Many studies of DT have been reported in other legume crop, such as soybean andMedicago truncatulawith little studies in the mungbean. In this study, the transcript profiles of mungbean seeds under different imbibition times were investigated for DT using RNA-sequencing (RNA-seq). A total of 3210 differentially expressed genes (DEGs) were found at the key period of DT (3-18 h of imbibition). Gene ontology (GO) and KEGG analysis showed that the terms of "response to stimulus," "transcription regulator," "methylation," and "starch and sucrose metabolism" were enriched for DT. Clustering analysis also showed that many transcription factors (MYB, AP2, and NAC), HSPs, embryogenesis abundant (LEA) proteins, and genes encoding methyltransferase and histone were differentially expressed. Nine of these DEGs were further validated by quantitative RT-PCR (qRT-PCR). Our study extends our knowledge of mungbean transcriptomes and further provides insight into the molecular mechanism of DT as well as new strategies for developing drought-tolerant crops.
authors.affiliation: College of Biology, Hunan UniversityChangsha, China; Key Laboratory of Plant Resource Conservation and Utilization of Hunan Province, Jishou UniversityJishou, China.
authors.forename: Xiangrong
authors.initials: X
authors.lastname: Tian
authors.affiliation: Key Laboratory of Plant Resource Conservation and Utilization of Hunan Province, Jishou University Jishou, China.
authors.forename: Sidi
authors.initials: S
authors.lastname: Li
authors.affiliation: Center of Analytical Service, Hunan Agricultural University Changsha, China.
authors.forename: Yisong
authors.initials: Y
authors.lastname: Liu
authors.affiliation: College of Biology, Hunan University Changsha, China.
authors.forename: Xuanming
authors.initials: X
authors.lastname: Liu
keywords: RNA-seq; desiccation tolerance; mungbean (Vigna radiate L. Wilczek); seeds; transcriptome