title: Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.
abstract: We demonstrate an accurate and sensitive quantification of mutated KRAS oncogene in genomic DNA, using droplet-based microfluidics and digital PCR.
authors.affiliation: CNRS, Univ. Bordeaux, CRPP, UPR 8641, 115 Avenue Schweitzer, F-33600, Pessac, France.
authors.forename: Deniz
authors.initials: D
authors.lastname: Pekin
authors.affiliation: INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, 45 rue des Saints-Pères, 75270, ParisCedex 06, France. valerie.taly@parisdescartes.fr.
authors.forename: Valerie
authors.initials: V
authors.lastname: Taly
mesh_terms: D000483:Alleles; D014408:Biomarkers, Tumor; D045744:Cell Line, Tumor; D004252:DNA Mutational Analysis / Q000295:instrumentation / Q000379:methods*; D004867:Equipment Design; D011905:Genes, ras / Q000235:genetics*; D006801:Humans; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D009154:Mutation; D009369:Neoplasms / Q000175:diagnosis / Q000235:genetics; D061848:Optical Imaging; D016133:Polymerase Chain Reaction / Q000379:methods; D060888:Real-Time Polymerase Chain Reaction; D012680:Sensitivity and Specificity; D013223:Statistics as Topic / Q000379:methods
chemical_list: D014408:Biomarkers, Tumor
keywords: Biomarkers; Cancer; Digital PCR; Droplet microfluidics; KRAS