title: Monitoring Vascular Regeneration and Xylem Connectivity in Arabidopsis thaliana.
abstract: Plants have a remarkable ability to regenerate vascular tissue after damage or wounding. A striking example of this phenomenon is the cutting and rejoining of plants during the process of grafting, which humans have used for millennia. Here, I describe how to graft Arabidopsis seedlings and how to monitor the vascular reconnection process during wound healing using fluorescent dyes and fluorescent proteins. Furthermore, I describe how to visualize xylem formation during graft healing. These techniques are useful for studying how the vascular system regenerates and for better understanding the process of plant grafting.
authors.affiliation: The Sainsbury Laboratory, University of Cambridge, Bateman Street, Cambridge, CB2 1LR, UK. cwm26@cam.ac.uk.
authors.forename: Charles W
authors.initials: CW
authors.lastname: Melnyk
mesh_terms: D017360:Arabidopsis / Q000502:physiology*; D008856:Microscopy, Fluorescence; D052585:Phloem / Q000254:growth & development* / Q000378:metabolism; D012038:Regeneration; D036226:Seedlings / Q000254:growth & development / Q000378:metabolism; D019737:Transplants; D052584:Xylem / Q000254:growth & development* / Q000378:metabolism
keywords: Arabidopsis thaliana; Chimeric plants; Fluorescent dyes; Micro-grafting; Vascular regeneration; Xylem formation