title: The experimental design approach to eluotropic strength of 20 solvents in thin-layer chromatography on silica gel.
abstract: The eluotropic strength on thin-layer silica plates was investigated for 20 chromatographic grade solvents available in current market. 35 model compounds were used as test subjects in the investigation. The use of modern mixture screening design allowed to estimate each solvent as a separate elution coefficient with an acceptable error of estimation (0.0913 of RMvalue). Additional bootstrapping technique was used to check the distribution and uncertainty of eluotropic estimates, proving very similar confidence intervals to linear regression. Principal component analysis proved that the only one parameter (mean eluotropic strength) is satisfactory to describe the solvent property, as it explains almost 90% of variance of retention. The obtained eluotropic data can be good appendix to earlier published results and their values can be interpreted in context of RMdifferences.
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland. Electronic address: lukasz.komsta@umlub.pl.
authors.forename: Łukasz
authors.initials: Ł
authors.lastname: Komsta
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland.
authors.forename: Barbara
authors.initials: B
authors.lastname: Stępkowska
authors.affiliation: Department of Medicinal Chemistry, Faculty of Pharmacy, Medical University of Lublin, Jaczewskiego 4, 20-090 Lublin, Poland.
authors.forename: Robert
authors.initials: R
authors.lastname: Skibiński
mesh_terms: D055598:Chemical Phenomena; D002855:Chromatography, Thin Layer / Q000379:methods*; D016001:Confidence Intervals; D006586:Hexanes / Q000737:chemistry; D016014:Linear Models; D012107:Research Design; D058428:Silica Gel / Q000737:chemistry*; D012997:Solvents / Q000737:chemistry*
chemical_list: D006586:Hexanes; D012997:Solvents; C026385:n-hexane; D058428:Silica Gel
keywords: Eluotropic strength; Mixture design; Silica gel; Thin-layer chromatography