title: [Zinc-induced interactions of the metal-binding domain of beta-amyloid with nucleic acids and glycosaminoglycans].
abstract: Zinc ions form complexes with β-amyloid peptides and play an important role in Alzheimer's disease pathogenesis. It has been demonstrated by turbidimetry and correlation spectroscopy that synthetic peptide Aβ16 representing the metal-binding domain of β-amyloid is able to interact with nucleic acids, chondroitin polysulfate, and dextran sulfates in the presence of zinc ions. The amino acid D7H substitution enhanced the peptide binding to polyanions, whereas the H6R and H6A-H13A substitutions abolished this interaction. It is suggested that the metal-binding domain may serve as a zinc-dependent site of β-amyloid interaction with biological polyanions including DNA, RNA, and glycosaminoglycans.
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991.|Orekhovich Institute of Biomedical Chemistry, Moscow, 119121.
authors.forename: S A
authors.initials: SA
authors.lastname: Khmeleva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991.
authors.forename: S A
authors.initials: SA
authors.lastname: Kozin
authors.affiliation: Orekhovich Institute of Biomedical Chemistry, Moscow, 119121.
authors.forename: Y Y
authors.initials: YY
authors.lastname: Kiseleva
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991.
authors.forename: V A
authors.initials: VA
authors.lastname: Mitkevich
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991.
authors.forename: A A
authors.initials: AA
authors.lastname: Makarov
authors.affiliation: Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, 119991.|Orekhovich Institute of Biomedical Chemistry, Moscow, 119121.|radkos@yandex.ru.
authors.forename: S P
authors.initials: SP
authors.lastname: Radko
mesh_terms: D016229:Amyloid beta-Peptides / Q000737:chemistry*; D004247:DNA / Q000737:chemistry*; D006025:Glycosaminoglycans / Q000737:chemistry*; D006801:Humans; D000072417:Protein Domains; D012313:RNA / Q000737:chemistry*; D015032:Zinc / Q000737:chemistry*
chemical_list: D016229:Amyloid beta-Peptides; D006025:Glycosaminoglycans; C020341:glucosaminoglycans; D012313:RNA; D004247:DNA; D015032:Zinc
keywords: Alzheimer disease; beta-amyloid; glycosaminoglycans; nucleic acids; zinc