title: Soybean cyclophilin GmCYP1 interacts with an isoflavonoid regulator GmMYB176.
abstract: Cyclophilins (CYPs) belong to the immunophilin superfamily with peptidyl-prolyl cis-trans isomerase (PPIase) activity. They catalyze the interconversion of the cis- and trans-rotamers of the peptidyl-prolyl amide bond of peptides. A yeast-two-hybrid screening using the isoflavonoid regulator GmMYB176 as bait identified GmCYP1 as one of the interacting proteins in soybean embryos. GmCYP1 localizes both in the nucleus and cytoplasm, and interacts in planta with GmMYB176, in the nucleus, and with SGF14l (a soybean 14-3-3 protein) in the nucleus and the cytoplasm. GmCYP1 contains a single cyclophilin-like domain and displays a high sequence identity with other plant CYPs that are known to have stress-specific function. Tissue-specific expression of GmCYP1 revealed higher expression in developing seeds compared to other vegetative tissues, suggesting their seed-specific role. Furthermore, GmCYP1 transcript level was reduced in response to stress. Since isoflavonoids are involved in plant stress resistance against biotic and abiotic factors, the interaction of GmCYP1 with the isoflavonoid regulators GmMYB176 and 14-3-3 protein suggests its role in defense in soybean.
authors.affiliation: Department of Biology, University of Western Ontario, 1151 Richmond St, London, ON, N6A 5B7, Canada.
authors.forename: Hemanta Raj
authors.initials: HR
authors.lastname: Mainali
authors.affiliation: Department of Biology, University of Western Ontario, 1151 Richmond St, London, ON, N6A 5B7, Canada.|London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada.
authors.forename: Arun Kumaran Anguraj
authors.initials: AK
authors.lastname: Vadivel
authors.affiliation: London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada.|College of Plant Science, Jilin University, Changchun, 130062, China.
authors.forename: Xuyan
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Biology, University of Western Ontario, 1151 Richmond St, London, ON, N6A 5B7, Canada.|London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada.
authors.forename: Mark
authors.initials: M
authors.lastname: Gijzen
authors.affiliation: Department of Biology, University of Western Ontario, 1151 Richmond St, London, ON, N6A 5B7, Canada.|London Research and Development Centre, Agriculture and Agri-Food Canada, 1391 Sandford Street, London, ON, N5V 4T3, Canada.
authors.forename: Sangeeta
authors.initials: S
authors.lastname: Dhaubhadel
mesh_terms: D048948:14-3-3 Proteins / Q000378:metabolism; D002467:Cell Nucleus / Q000737:chemistry / Q000378:metabolism; D021983:Cyclophilins / Q000378:metabolism*; D003593:Cytoplasm / Q000737:chemistry / Q000378:metabolism; D030262:Soybean Proteins / Q000302:isolation & purification / Q000378:metabolism*; D013025:Glycine max / Q000378:metabolism*; D013312:Stress, Physiological
chemical_list: D048948:14-3-3 Proteins; D030262:Soybean Proteins; D021983:Cyclophilins