title: Prognostic efficacy of the human B-cell lymphoma prognostic genes in predicting disease-free survival (DFS) in the canine counterpart.
abstract: BACKGROUND
  Canine B-cell lymphoma is deemed an ideal model of human non-Hodgkin's lymphoma where the lymphomas of both species share similar clinical features and biological behaviors. However there are some differences between tumor features in both species. In the current study, we sought to evaluate the prognostic efficacy of human B-cell lymphoma prognostic gene signatures in canine B-cell lymphoma.
  
  
  METHODS
  The corresponding probe sets of 36 human B-cell lymphoma prognostic genes were retrieved from 2 canine B-cell lymphoma microarray datasets (GSE43664 and GSE39365) (76 samples), and prognostic probe sets were thereafter detected using the univariate and multivariate Cox proportional-hazard model and the Kaplan-Meier analysis. The two datasets were employed both as training sets and as external validation sets for each other. Results were confirmed using quantitative real-time PCR (qRT-PCR) analysis.
  
  
  RESULTS
  In the univariate analysis, CCND1, CCND2, PAX5, CR2, LMO2, HLA-DQA1, P53, CD38, MYC-N, MYBL1, and BIRCS5 were associated with longer disease-free survival (DFS), while CD44, PLAU, and FN1 were allied to shorter DFS. However, the multivariate Cox proportional-hazard analysis confirmed CCND1 and BIRCS5 as prognostic genes for canine B-cell lymphoma. qRT-PCR used for verification of results indicated that expression level of CCND1 was significantly higher in B-cell lymphoma patients with the long DFS than ones with the short DFS, while expression level of BIRCS5 wasn't significantly different between two groups.
  
  
  CONCLUSIONS
  Our results confirmed CCND1 as important gene that can be used as a potential predictor in this tumor type.
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, P.O Box: 76169133, Kerman, Iran. zamani_2012@alumni.ut.ac.ir.
authors.forename: Mohamad
authors.initials: M
authors.lastname: Zamani-Ahmadmahmudi
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran.
authors.forename: Sina
authors.initials: S
authors.lastname: Aghasharif
authors.affiliation: Department of Clinical Science, Faculty of Veterinary Medicine, Islamic Azad University, Garmsar Branch, Garmsar, Iran.
authors.forename: Keyhan
authors.initials: K
authors.lastname: Ilbeigi
mesh_terms: D000818:Animals; D014408:Biomarkers, Tumor; D019938:Cyclin D1 / Q000235:genetics / Q000378:metabolism; D018572:Disease-Free Survival; D004283:Dog Diseases / Q000235:genetics* / Q000473:pathology; D004285:Dogs; D005786:Gene Expression Regulation / Q000502:physiology*; D020022:Genetic Predisposition to Disease; D006801:Humans; D016393:Lymphoma, B-Cell / Q000235:genetics / Q000662:veterinary*; D020411:Oligonucleotide Array Sequence Analysis
chemical_list: D014408:Biomarkers, Tumor; D019938:Cyclin D1
keywords: Canine B-cell lymphoma; Cox proportional-hazard analysis; Prognosis; Survival