title: [Effects of laser solid forming of porous titanium on proliferation of osteoblast and RANKL/OPG expression].
abstract: To evaluate the effect of laser solid forming (LSF) of porous titanium on receptor activator of NF-κB ligand (RANKL)/osteoprorotegerin (OPG) expression and osteoblast cells growth.  Methods: The DMEM and sterile saline were used for porous titanium extract. The osteoblast cells were cultured in the extract while equal amount of  DMEM and sterile saline were added to the control group. The growth of the cells were observed under an inverted phase contrast microscope. MTT was used to detect the growth inhibitory rates. The adhesion capacity of osteoblasts were measured. The growth in the material surface was examined by the electron microscope, and the expressions of RANKL and OPG were determined by Westen blot.  Results: At the first day, the osteoblast proliferation rate was significantly different (P<0.05), at the fourth and seventh day, the osteoblast proliferation rate was not significantly affected in the LSF group (P>0.05); at each time point, the osteoblast proliferation rate were significantly different between the two groups (P<0.05). Compared with the control group, RANKL and OPG protein expression were not significantly different (P>0.05). The laser solid forming of porous titanium showed well bone compatibility.  Conclusion: The porous titanium did not affect osteoblast proliferation due to its well bone compatibility. It did not affect the OPG/RANKL/RANK-axis system of bone metabolism, exibiting a wide applicable prospect for tissue engineering.
authors.affiliation: Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Hui
authors.initials: H
authors.lastname: Chen
authors.affiliation: Physical Examination Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Shanshan
authors.initials: S
authors.lastname: Du
authors.affiliation: Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Ping
authors.initials: P
authors.lastname: Zhen
authors.affiliation: Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Xusheng
authors.initials: X
authors.lastname: Li
authors.affiliation: Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Xiaoyan
authors.initials: X
authors.lastname: Liang
authors.affiliation: Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Lijuan
authors.initials: L
authors.lastname: Fan
authors.affiliation: Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Jie
authors.initials: J
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi'an 710072, China.
authors.forename: Haiou
authors.initials: H
authors.lastname: Yang
authors.affiliation: Department of Joint Surgery, Orthopaedics Center, Lanzhou General Hospital of PLA, Lanzhou 730050, China.
authors.forename: Jun
authors.initials: J
authors.lastname: Liu
mesh_terms: D001672:Biocompatible Materials / Q000737:chemistry; D002448:Cell Adhesion / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects*; D002478:Cells, Cultured; D003470:Culture Media / Q000737:chemistry; D008024:Ligands; D010006:Osteoblasts / Q000187:drug effects*; D010012:Osteogenesis / Q000187:drug effects*; D053244:Osteoprotegerin / Q000378:metabolism; D016062:Porosity; D053246:Receptor Activator of Nuclear Factor-kappa B / Q000378:metabolism; D023822:Tissue Engineering / Q000295:instrumentation*; D054457:Tissue Scaffolds / Q000737:chemistry*; D014025:Titanium / Q000494:pharmacology*
chemical_list: D001672:Biocompatible Materials; D003470:Culture Media; D008024:Ligands; D053244:Osteoprotegerin; D053246:Receptor Activator of Nuclear Factor-kappa B; D014025:Titanium