title: Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip.
abstract: The enumeration and analysis of circulating tumor cells (CTCs) is an increasing interest for monitoring disease progression or response to treatment, specifically as a companion diagnostic for new anticancer drugs, and for research into the mechanisms of disease progression and metastases. Ideally, CTCs would be enriched from very small samples, with minimal handling, high recovery, and no requirement for the expression of specific surface markers. Here, we describe negative enrichment as the preferred approach for cancer cell isolation using a microfluidic platform.
authors.affiliation: Institut Curie, PSL Research University, CNRS, UMR 168, 26 rue d'Ulm, 75005, Paris, France. hamizah.cognart@cri-paris.org.|Sorbonne Universités, UPMC Univ Paris 06, CNRS, UMR 168, Paris, France. hamizah.cognart@cri-paris.org.
authors.forename: Hamizah A
authors.initials: HA
authors.lastname: Cognart
authors.affiliation: BioElectronics Programme, Institute of Microelectronics, A*STAR (Agency for Science, Technology and Research), 1 Science Park Road, Singapore, 117528, Singapore.
authors.forename: Chia-Pin
authors.initials: CP
authors.lastname: Chang
mesh_terms: D045744:Cell Line, Tumor; D004867:Equipment Design; D006801:Humans; D007150:Immunohistochemistry / Q000379:methods; D018189:Immunomagnetic Separation / Q000379:methods; D046210:Microfluidic Analytical Techniques / Q000295:instrumentation / Q000379:methods*; D044085:Microfluidics / Q000295:instrumentation / Q000379:methods*; D008856:Microscopy, Fluorescence; D025202:Molecular Diagnostic Techniques / Q000295:instrumentation / Q000379:methods*; D009369:Neoplasms / Q000175:diagnosis; D009360:Neoplastic Cells, Circulating; D061848:Optical Imaging
keywords: Cancer cell isolation; Circulating tumor cells (CTCs); Metastasis; Microfluidic; Negative enrichment; White blood cell (WBC) depletion