title: Ultra-sensitive detection of kanamycin for food safety using a reduced graphene oxide-based fluorescent aptasensor.
abstract: Overuse of antibiotics has caused serious problems, such as appearance of super bacteria, whose accumulation in the human body through the food chain is a concern. Kanamycin is a common antibiotic used to treat diverse infections; however, residual kanamycin can cause many side effects in humans. Thus, development of an ultra-sensitive, precise, and simple detection system for residual kanamycin in food products is urgently needed for food safety. In this study, we identified kanamycin-binding aptamers via a new screening method, and truncated variants were analyzed for optimization of the minimal sequence required for target binding. We found various aptamers with high binding affinity from 34.7 to 669 nanomolar Kdappvalues with good specificity against kanamycin. Furthermore, we developed a reduced graphene oxide (RGO)-based fluorescent aptasensor for kanamycin detection. In this system, kanamycin was detected at a concentration as low as 1 pM (582.6 fg/mL). In addition, this method could detect kanamycin accurately in kanamycin-spiked blood serum and milk samples. Consequently, this simple, rapid, and sensitive kanamycin detection system with newly structural and functional analysis aptamer exhibits outstanding detection compared to previous methods and provides a new possibility for point of care testing and food safety.
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Na-Reum
authors.initials: NR
authors.lastname: Ha
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: In-Pil
authors.initials: IP
authors.lastname: Jung
authors.affiliation: Food Safety Center, Lotte Confectionery Co., Ltd., Seoul 07207, Republic of Korea.
authors.forename: Im-Joung
authors.initials: IJ
authors.lastname: La
authors.affiliation: Institute of Advanced Machinery and Design, Department of Mechanical and Aerospace Engineering, Seoul National University, Seoul 08826, Republic of Korea.
authors.forename: Ho-Sup
authors.initials: HS
authors.lastname: Jung
authors.affiliation: Department of Chemistry and Research Institute of Natural Sciences, Hanyang University, Seoul 04763, Republic of Korea.
authors.forename: Moon-Young
authors.initials: MY
authors.lastname: Yoon