title: Solubilization of Membrane Proteins into Functional Lipid-Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer.
abstract: Once removed from their natural environment, membrane proteins depend on membrane-mimetic systems to retain their native structures and functions. To this end, lipid-bilayer nanodiscs that are bounded by scaffold proteins or amphiphilic polymers such as styrene/maleic acid (SMA) copolymers have been introduced as alternatives to detergent micelles and liposomes for in vitro membrane-protein research. Herein, we show that an alternating diisobutylene/maleic acid (DIBMA) copolymer shows equal performance to SMA in solubilizing phospholipids, stabilizes an integral membrane enzyme in functional bilayer nanodiscs, and extracts proteins of various sizes directly from cellular membranes. Unlike aromatic SMA, aliphatic DIBMA has only a mild effect on lipid acyl-chain order, does not interfere with optical spectroscopy in the far-UV range, and does not precipitate in the presence of low millimolar concentrations of divalent cations.
authors.affiliation: Molecular Biophysics, University of Kaiserslautern, Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.|Department of Chemistry, University of Ibadan, 200284, Ibadan, Nigeria.
authors.forename: Abraham Olusegun
authors.identifier: 0000-0001-8647-4781
authors.initials: AO
authors.lastname: Oluwole
authors.affiliation: Molecular Biophysics, University of Kaiserslautern, Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.
authors.forename: Bartholomäus
authors.initials: B
authors.lastname: Danielczak
authors.affiliation: Institute of Chemistry and Institute of Biochemistry and Biotechnology, Martin Luther University Halle-Wittenberg, Von-Danckelmann-Platz 4, 06120, Halle, Germany.
authors.forename: Annette
authors.initials: A
authors.lastname: Meister
authors.affiliation: Department of Chemistry, University of Ibadan, 200284, Ibadan, Nigeria.
authors.forename: Jonathan Oyebamiji
authors.identifier: 0000-0002-1407-6677
authors.initials: JO
authors.lastname: Babalola
authors.affiliation: Molecular Biophysics, University of Kaiserslautern, Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.
authors.forename: Carolyn
authors.initials: C
authors.lastname: Vargas
authors.affiliation: Molecular Biophysics, University of Kaiserslautern, Erwin-Schrödinger-Str. 13, 67663, Kaiserslautern, Germany.
authors.forename: Sandro
authors.identifier: 0000-0001-5469-8772
authors.initials: S
authors.lastname: Keller
mesh_terms: D000475:Alkenes / Q000737:chemistry*; D003902:Detergents / Q000737:chemistry; D004926:Escherichia coli / Q000737:chemistry; D029968:Escherichia coli Proteins / Q000737:chemistry / Q000302:isolation & purification; D008051:Lipid Bilayers / Q000737:chemistry*; D008081:Liposomes / Q000737:chemistry; D008298:Maleates / Q000737:chemistry*; D008565:Membrane Proteins / Q000737:chemistry* / Q000302:isolation & purification; D008823:Micelles; D049329:Nanostructures / Q000737:chemistry; D010743:Phospholipids / Q000737:chemistry; D011108:Polymers / Q000737:chemistry*; D012995:Solubility
chemical_list: D000475:Alkenes; D003902:Detergents; D029968:Escherichia coli Proteins; D008051:Lipid Bilayers; D008081:Liposomes; D008298:Maleates; D008565:Membrane Proteins; D008823:Micelles; D010743:Phospholipids; D011108:Polymers; C030272:maleic acid; C032725:diisobutylene
keywords: amphiphilic polymers; biomembranes; membrane mimetics; membrane proteins; protein chromatography