title: Preparation of a Specific ssDNA Aptamer for Brevetoxin-2 Using SELEX.
abstract: The existing assays for detecting brevetoxin (BTX) depend on expensive equipment with a professional operator or on an antibody with limited stability, which requires complex processes, a high cost, and a considerable amount of time. The development of an alternative detection probe is another promising research direction. This paper reports the use of aptamers binding to BTX-2 in an analytical assay using the systematic evolution of ligands by exponential enrichment (SELEX). After 12 rounds of selection, the secondary structures of 25 sequences were predicted. Compared to other aptamers, Bap5 has relatively high affinity with the lowest dissociation constant of 4.83μM, and IC50is 73.81 ng mL-1. A good linear regression formula ofy= 30.688x- 7.329 with a coefficient correlation ofR2= 0.9798 was obtained using a biotin-avidin ELISA. Moreover, there is no cross-reaction with the detected marine toxins, except for BTX-2. Thus, Bap5 has potential to detect BTX-2 in shellfish in the future as a substitute for the recognition probe.
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Rui-Yun
authors.initials: RY
authors.lastname: Tian
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China; Emergency Department, The Eastern Division, The First Hospital of Jilin University, Changchun 130062, China.
authors.forename: Chao
authors.identifier: 0000-0003-1483-3815
authors.initials: C
authors.lastname: Lin
authors.affiliation: Fuqing Entry-Exit Inspection and Quarantine Bureau, Port District, Qingrong Road, Fuqing, Fujian 350300, China.
authors.forename: Shi-Yu
authors.initials: SY
authors.lastname: Yu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Sheng
authors.initials: S
authors.lastname: Gong
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Pan
authors.initials: P
authors.lastname: Hu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Yan-Song
authors.initials: YS
authors.lastname: Li
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Zong-Cheng
authors.initials: ZC
authors.lastname: Wu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Gao
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Yu
authors.initials: Y
authors.lastname: Zhou
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Zeng-Shan
authors.initials: ZS
authors.lastname: Liu
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Hong-Lin
authors.identifier: 0000-0002-3364-8335
authors.initials: HL
authors.lastname: Ren
authors.affiliation: Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun 130062, China.
authors.forename: Shi-Ying
authors.identifier: 0000-0002-8325-1811
authors.initials: SY
authors.lastname: Lu