title: Novel Bioengineered Three-Dimensional Human Intestinal Model for Long-Term Infection of Cryptosporidium parvum.
abstract: Cryptosporidiumspp. are apicomplexan parasites of global importance that cause human diarrheal disease.In vitroculture models that may be used to study this parasite and that have physiological relevance toin vivoinfection remain suboptimal. Thus, the pathogenesis of cryptosporidiosis remains poorly characterized, and interventions for the disease are limited. In this study, we evaluated the potential of a novel bioengineered three-dimensional (3D) human intestinal tissue model (which we developed previously) to support long-term infection byCryptosporidium parvumInfection was assessed by immunofluorescence assays and confocal and scanning electron microscopy and quantified by quantitative reverse transcription-PCR. We found thatC. parvuminfected and developed in this tissue model for at least 17 days, the extent of the study time used in the present study. Contents from infected scaffolds could be transferred to fresh scaffolds to establish new infections for at least three rounds. Asexual and sexual stages and the formation of new oocysts were observed during the course of infection. Additionally, we observed ablation, blunting, or distortion of microvilli in infected epithelial cells. Ultimately, a 3D model system capable of supporting continuousCryptosporidiuminfection will be a useful tool for the study of host-parasite interactions, identification of putative drug targets, screening of potential interventions, and propagation of genetically modified parasites.
authors.affiliation: Tufts University Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts, USA.|Tufts Medical Center, Boston, Massachusetts, USA.
authors.forename: Maria A
authors.initials: MA
authors.lastname: DeCicco RePass
authors.affiliation: Tufts University School of Engineering, Medford, Massachusetts, USA.
authors.forename: Ying
authors.initials: Y
authors.lastname: Chen
authors.affiliation: Tufts University School of Engineering, Medford, Massachusetts, USA.
authors.forename: Yinan
authors.initials: Y
authors.lastname: Lin
authors.affiliation: Tufts University School of Engineering, Medford, Massachusetts, USA.
authors.forename: Wenda
authors.initials: W
authors.lastname: Zhou
authors.affiliation: Tufts University Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts, USA.|Tufts University School of Engineering, Medford, Massachusetts, USA.
authors.forename: David L
authors.initials: DL
authors.lastname: Kaplan
authors.affiliation: Tufts University Sackler School of Graduate Biomedical Sciences, Boston, Massachusetts, USA hward@tuftsmedicalcenter.org.|Tufts Medical Center, Boston, Massachusetts, USA.
authors.forename: Honorine D
authors.initials: HD
authors.lastname: Ward
mesh_terms: D000818:Animals; D057005:Bioengineering; D002460:Cell Line; D003457:Cryptosporidiosis / Q000469:parasitology*; D016785:Cryptosporidium parvum / Q000502:physiology*; D004847:Epithelial Cells; D006801:Humans; D066298:In Vitro Techniques; D007422:Intestines / Q000469:parasitology* / Q000648:ultrastructure; D046509:Tissue Culture Techniques; D054457:Tissue Scaffolds
keywords: 3D model; Cryptosporidium; in vitro culture; intestinal epithelial cells