title: Tricalcium phosphate solubilization and nitrogen fixation by newly isolated Aneurinibacillus aneurinilyticus CKMV1 from rhizosphere of Valeriana jatamansi and its growth promotional effect.
abstract: Aneurinibacillus aneurinilyticus strain CKMV1 was isolated from rhizosphere of Valeriana jatamansi and possessed multiple plant growth promoting traits like production of phosphate solubilization (260mg/L), nitrogen fixation (202.91nmolethylenemL-1h-1), indole-3-acetic acid (IAA) (8.1μg/mL), siderophores (61.60%), HCN (hydrogen cyanide) production and antifungal activity. We investigated the ability of isolate CKMV1 to solubilize insoluble P via mechanism of organic acid production. High-performance liquid chromatography (HPLC) study showed that isolate CKMV1 produced mainly gluconic (1.34%) and oxalic acids. However, genetic evidences for nitrogen fixation and phosphate solubilization by organic acid production have been reported first time for A. aneurinilyticus strain CKMV1. A unique combination of glucose dehydrogenase (gdh) gene and pyrroloquinoline quinone synthase (pqq) gene, a cofactor of gdh involved in phosphate solubilization has been elucidated. Nitrogenase (nif H) gene for nitrogen fixation was reported from A. aneurinilyticus. It was notable that isolate CKMV1 exhibited highest antifungal against Sclerotium rolfsii (93.58%) followed by Fusarium oxysporum (64.3%), Dematophora necatrix (52.71%), Rhizoctonia solani (91.58%), Alternaria sp. (71.08%) and Phytophthora sp. (71.37%). Remarkable increase was observed in seed germination (27.07%), shoot length (42.33%), root length (52.6%), shoot dry weight (62.01%) and root dry weight (45.7%) along with NPK (0.74, 0.36, 1.82%) content of tomato under net house condition. Isolate CKMV1 possessed traits related to plant growth promotion, therefore, could be a potential candidate for the development of biofertiliser or biocontrol agent and this is the first study to include the Aneurinibacillus as PGPR.
authors.affiliation: Dr Y. S. Parmar University of Horticulture and Forestry, Department of Basic Sciences Microbiology Section, Nauni, Solan, India.
authors.forename: Anjali
authors.initials: A
authors.lastname: Chauhan
authors.affiliation: Lovely Professional University, Department of Microbiology, Jalandhar, Punjab, India.
authors.forename: Shiwani
authors.initials: S
authors.lastname: Guleria
authors.affiliation: Punjabi University Patiala, Department of Biotechnology, Patiala, Punjab, India.
authors.forename: Praveen P
authors.initials: PP
authors.lastname: Balgir
authors.affiliation: DAV University, Department of Microbiology, Jalandhar, Punjab, India.
authors.forename: Abhishek
authors.initials: A
authors.lastname: Walia
authors.affiliation: Jaypee University of Information Technology, Waknaghat, Himachal Pradesh, India.
authors.forename: Rishi
authors.initials: R
authors.lastname: Mahajan
authors.affiliation: DBT-IOC Centre for Bioenergy Research, Indian Oil R&D Centre, Faridabad, Haryana, India.
authors.forename: Preeti
authors.initials: P
authors.lastname: Mehta
authors.affiliation: Dr Y. S. Parmar University of Horticulture and Forestry, Department of Basic Sciences, Nauni, Solan, India. Electronic address: profckshirkot@gmail.com.
authors.forename: Chand Karan
authors.initials: CK
authors.lastname: Shirkot
mesh_terms: D000898:Antibiosis; D056449:Bacillales / Q000302:isolation & purification* / Q000378:metabolism; D018533:Biomass; D002130:Calcium Phosphates / Q000378:metabolism*; D002851:Chromatography, High Pressure Liquid; D005658:Fungi / Q000254:growth & development; D018551:Solanum lycopersicum / Q000254:growth & development* / Q000382:microbiology; D009586:Nitrogen Fixation; D010937:Plant Growth Regulators / Q000378:metabolism*; D018517:Plant Roots / Q000382:microbiology; D058441:Rhizosphere; D012988:Soil Microbiology; D014632:Valerian / Q000382:microbiology*
chemical_list: D002130:Calcium Phosphates; D010937:Plant Growth Regulators; C018392:tricalcium phosphate
keywords: Aneurinibacillus aneurinilyticus; Glucose dehydrogenase; Nitrogen fixation; PGPR; Phosphate solubilization; Valeriana jatamansi