title: Global Lysine Acetylome Analysis of Desiccated Somatic Embryos ofPicea asperata.
abstract: Partial desiccation treatment (PDT) promotes the germination capacity of conifer somatic embryos. Lysine acetylation (LysAc) is a dynamic and reversible post-translational modification that plays a key role in many biological processes including metabolic pathways and stress response. To investigate the functional impact of LysAc in the response ofPicea asperatasomatic embryos to PDT, we performed a global lysine acetylome analysis. Here, combining antibody-based affinity enrichment and high-resolution mass spectrometry, we identified and validated 1079 acetylation sites in 556 acetylated proteins fromP. asperatasomatic embryos during PDT. These data represent a novel large-scale dataset of lysine-acetylated proteins from the conifer family. Intensive bioinformatics analysis of the Gene Ontology of molecular functions demonstrated that lysine-acetylated proteins were mainly associated with binding, catalytic activities, and structural molecular activities. Functional characterization of the acetylated proteins revealed that in the desiccated somatic embryos, LysAc is mainly involved in the response to stress and central metabolism. Accordingly, the majority of these interacting proteins were also highly enriched in ribosome, proteasome, spliceosome, and carbon metabolism clusters. This work provides the most comprehensive profile of LysAc for a coniferous species obtained to date and facilitates the systematic study of the physiological role of LysAc in desiccated somatic embryos ofP. asperata.
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
authors.forename: Yan
authors.initials: Y
authors.lastname: Xia
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
authors.forename: Danlong
authors.initials: D
authors.lastname: Jing
authors.affiliation: Centre for Forest Biology, Department of Biology, University of Victoria Victoria, BC, Canada.
authors.forename: Lisheng
authors.initials: L
authors.lastname: Kong
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
authors.forename: Jianwei
authors.initials: J
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
authors.forename: Fangqun
authors.initials: F
authors.lastname: OuYang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University Harbin, China.
authors.forename: Hanguo
authors.initials: H
authors.lastname: Zhang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
authors.forename: Junhui
authors.initials: J
authors.lastname: Wang
authors.affiliation: State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry Beijing, China.
authors.forename: Shougong
authors.initials: S
authors.lastname: Zhang
keywords: conifer; lysine acetylome; partial desiccation treatment; response to stress; somatic embryo