title: Fabrication of nonfouling, bactericidal, and bacteria corpse release multifunctional surface through surface-initiated RAFT polymerization.
abstract: Infections after surgery or endophthalmitis are potentially blinding complications caused by bacterial adhesion and subsequent biofilm formation on the intraocular lens. Neither single-function anti-adhesion surface nor contacting killing surface can exhibit ideal antibacterial function. In this work, a novel (2-(dimethylamino)-ethyl methacrylate-co-2-methacryloyloxyethyl phosphorylcholine) (p (DMAEMA-co-MPC)) brush was synthesized by "grafting from" method through reversible-addition fragmentation chain transfer polymerization. 1-Bromoheptane was used to quaternize the p (DMAEMA-co-MPC) brush coating and to endow the surface with bactericidal function. The success of the surface functionalization was confirmed by atomic force microscopy, water contact angle, and spectroscopic ellipsometry. The quaternary ammonium salt units were employed as efficient disinfection that can eliminate bacteria through contact killing, whereas the 2-methacryloyloxyethyl phosphorylcholine units were introduced to suppress unwanted nonspecific adsorption. The functionalized poly(dimethyl siloxane) surfaces showed efficiency in reducing bovine serum albumin adsorption and in inhibiting bacteria adhesion and biofilm formation. The copolymer brushes also demonstrated excellent bactericidal function against gram-positive (Staphylococcus aureus) bacteria measured by bacteria live/dead staining and shake-flask culture methods. The surface biocompatibility was evaluated by morphology and activity measurement with human lens epithelial cells in vitro. The achievement of the p (DMAEMA+-co-MPC) copolymer brush coating with nonfouling, bactericidal, and bacteria corpse release properties can be used to modify intraocular lenses.
authors.affiliation: School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People's Republic of China.
authors.forename: Bailiang
authors.initials: B
authors.lastname: Wang
authors.affiliation: School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou.
authors.forename: Zi
authors.initials: Z
authors.lastname: Ye
authors.affiliation: School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou.
authors.forename: Yihong
authors.initials: Y
authors.lastname: Tang
authors.affiliation: School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou.
authors.forename: Yuemei
authors.initials: Y
authors.lastname: Han
authors.affiliation: School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People's Republic of China.
authors.forename: Quankui
authors.initials: Q
authors.lastname: Lin
authors.affiliation: Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People's Republic of China.
authors.forename: Huihua
authors.initials: H
authors.lastname: Liu
authors.affiliation: School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People's Republic of China.
authors.forename: Hao
authors.initials: H
authors.lastname: Chen
authors.affiliation: School of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou; Wenzhou Institute of Biomaterials and Engineering, Chinese Academy of Sciences, Wenzhou, People's Republic of China.
authors.forename: Kaihui
authors.initials: K
authors.lastname: Nan
mesh_terms: D000327:Adsorption; D000900:Anti-Bacterial Agents / Q000737:chemistry*; D001422:Bacterial Adhesion / Q000187:drug effects*; D001424:Bacterial Infections / Q000188:drug therapy*; D061249:Batch Cell Culture Techniques; D002470:Cell Survival; D004129:Dimethylpolysiloxanes; D009877:Endophthalmitis / Q000188:drug therapy; D004847:Epithelial Cells / Q000187:drug effects / Q000382:microbiology; D006801:Humans; D007908:Lens, Crystalline / Q000187:drug effects / Q000382:microbiology; D008689:Methacrylates / Q000737:chemistry*; D018625:Microscopy, Atomic Force; D008856:Microscopy, Fluorescence; D010767:Phosphorylcholine / Q000031:analogs & derivatives* / Q000737:chemistry; D058105:Polymerization; D011108:Polymers / Q000737:chemistry*; D011183:Postoperative Complications; D013203:Staphylococcal Infections / Q000188:drug therapy; D013211:Staphylococcus aureus; D013499:Surface Properties
chemical_list: D000900:Anti-Bacterial Agents; D004129:Dimethylpolysiloxanes; D008689:Methacrylates; D011108:Polymers; D010767:Phosphorylcholine; C070638:2-methacryloyloxyethyl phosphorylcholine; C501844:dimethicone; C049840:2-(dimethylamino)ethyl methacrylate
keywords: anti-fouling; biofilms; endophthalmitis; intraocular lenses; poly(dimethylsiloxane)