title: Analytical performances of Optilite®turbidimeter (The Binding Site): a new dedicated analyser for specific proteins determination.
abstract: We checked analytical performances of Optilite®analyser for immunoglobulins G, A, M, subclasses of IgG, free light chains of Ig (Freelite®) and complement's fractions using Binding Site reagents. CVs for repeteability and reproducibility showed very good results, respectively <3% and <10% for all tested parameters, in agreement with Ricos and SFBC recommendations. Comparisons with results obtained on BN™II (Siemens) or SPAPLUS ® (Binding site) analysers showed a good agreement (>83%) according to Bland and Altman analysis. Sample throughput with either a batch of Freelite®only or Freelite®and immunoglobulins showed a gain of total realisation time on Optilite®versus BN™II. Optilite®analyser performed automatic dilutions until result and antigen excess determination for parameters as Freelite®or IgG4. In terms of practicability, traceability and maintenance, Optilite®turbidimeter alone or connected to LIS via DataSite software is well adapted to specialized laboratory for proteins determinations.
authors.affiliation: Laboratoire d'immunochimie et auto-immunité, Département d'immunologie, Groupe Hospitalier Pitié-Salpêtrière-Charles Foix, APHP, France.
authors.forename: Pascale
authors.initials: P
authors.lastname: Ghillani
authors.affiliation: Laboratoire d'immunochimie et auto-immunité, Département d'immunologie, Groupe Hospitalier Pitié-Salpêtrière-Charles Foix, APHP, France.
authors.forename: Laurent
authors.initials: L
authors.lastname: Dufat
authors.affiliation: Laboratoire d'immunochimie et auto-immunité, Département d'immunologie, Groupe Hospitalier Pitié-Salpêtrière-Charles Foix, APHP, France.
authors.forename: Delphine
authors.initials: D
authors.lastname: Sterlin
authors.affiliation: Laboratoire d'immunochimie et auto-immunité, Département d'immunologie, Groupe Hospitalier Pitié-Salpêtrière-Charles Foix, APHP, France.
authors.forename: Lucile
authors.initials: L
authors.lastname: Musset
mesh_terms: D057205:Automation, Laboratory / Q000295:instrumentation* / Q000592:standards; D001665:Binding Sites; D019411:Clinical Laboratory Techniques / Q000295:instrumentation / Q000379:methods / Q000592:standards; D006801:Humans; D007136:Immunoglobulins / Q000032:analysis / Q000378:metabolism*; D007159:Immunologic Tests / Q000295:instrumentation / Q000379:methods; D057230:Limit of Detection; D009391:Nephelometry and Turbidimetry / Q000295:instrumentation / Q000379:methods; D011506:Proteins / Q000032:analysis* / Q000737:chemistry / Q000378:metabolism*; D011933:Reagent Kits, Diagnostic / Q000592:standards; D015203:Reproducibility of Results; D012680:Sensitivity and Specificity; D012984:Software / Q000592:standards
chemical_list: D007136:Immunoglobulins; D011506:Proteins; D011933:Reagent Kits, Diagnostic
keywords: DataSite software; Optilite® analyser; alytical performances; immunoglobulins; turbidimetry