title: Resuscitation of starved suspended- and attached-growth anaerobic ammonium oxidizing bacteria with and without acetate.
abstract: Anammox application for nutrient removal from wastewater is increasing, though questions remain about anammox resilience to fluctuating conditions. Resuscitation of anammox suspended- and attached-growth cultures after 3 months of starvation was studied with and without acetate dosing. Without acetate, the attached-growth culture recovered more quickly than the suspended-growth culture. Suspended-growth cultures recovered more quickly (within 60 days) with weekly and daily acetate dosing than without, but anammox activity and copy numbers decreased with continued acetate addition. All attached-growth cultures recovered within 60 days, but after that activity with acetate dosing was consistently at least 20% lower than that without acetate addition. Ca. Jettenia caeni, Ca. Anammoxoglobus sp., Ca. Brocadia fulgida, Ca. Brocadia anammoxidans, Ca. Brocadia fulgida and Ca. Jettenia asiatica were identified. Acetate addition can significantly accelerate short-term resuscitation of enriched anammox suspended-growth cultures after starvation but may reduce anammox activity over the longer term in suspended- and attached-growth cultures.
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand E-mail: pongsak.n@ku.ac.th.
authors.forename: Supaporn
authors.initials: S
authors.lastname: Phanwilai
authors.affiliation: Center of Excellence on Hazardous Substance Management (HSM) and Department of Environmental Engineering, Faculty of Engineering, King Mongkut's University of Technology Thonburi, Bangkok 10140, Thailand.
authors.forename: Chalermraj
authors.initials: C
authors.lastname: Wantawin
authors.affiliation: Department of Chemical Engineering, Faculty of Engineering, Tokyo University of Agriculture and Technology, Tokyo, Japan.
authors.forename: Akihiko
authors.initials: A
authors.lastname: Terada
authors.affiliation: Department of Environmental Engineering, Faculty of Engineering, Kasetsart University, Bangkok 10900, Thailand E-mail: pongsak.n@ku.ac.th.
authors.forename: Pongsak Lek
authors.initials: PL
authors.lastname: Noophan
authors.affiliation: Engineering Research Center (ERC) for Re-inventing the Nation's Urban Water Infrastructure (ReNUWIt) and Civil and Environmental Engineering Department, Colorado School of Mines, Golden, CO, USA.
authors.forename: Junko
authors.initials: J
authors.lastname: Munakata-Marr
mesh_terms: D000085:Acetates / Q000378:metabolism*; D064751:Ammonium Compounds / Q000737:chemistry*; D001421:Bacteria, Anaerobic / Q000502:physiology*; D010084:Oxidation-Reduction; D000644:Quaternary Ammonium Compounds; D014865:Waste Disposal, Fluid / Q000379:methods; D062065:Wastewater / Q000382:microbiology; D014874:Water Pollutants, Chemical / Q000737:chemistry / Q000378:metabolism
chemical_list: D000085:Acetates; D064751:Ammonium Compounds; D000644:Quaternary Ammonium Compounds; D062065:Wastewater; D014874:Water Pollutants, Chemical