title: CD9 monoclonal antibody-conjugated PEGylated liposomes for targeted delivery of rapamycin in the treatment of cellular senescence.
abstract: Premature cellular senescence refers to the state of irreversible cell cycle arrest due to DNA damage or other stresses. In this study, CD9 monoclonal antibody (CD9mAb) was successfully conjugated to the surface of PEGylated liposomes for targeted delivery of rapamycin (LR-CD9mAb) to overcome senescence of CD9 receptor-overexpressing cells. LR-CD9mAb has a small particle size (143.3 ± 2.4 nm), narrow size distribution (polydispersity index: 0.220 ± 0.036), and negative zeta potential (-14.6 ± 1.2 mV). The uptake of CD9-targeted liposomes by premature senescent human dermal fibroblasts (HDFs) was higher than that by young HDFs, as displayed by confocal microscopic images. The senescence might not be reversed by treatment with rapamycin; however, the drug promoted cell proliferation and reduced the number of cells that expressed the senescence-associated-β-galactosidase (SA-β-gal). These effects were further confirmed by cell viability, cell cycle, and Western blotting analyses. Moreover, CD9-targeted liposomes showed better anti-senescence activity, in comparison with free rapamycin or the conventional liposomal formulation, suggesting the potential application of this system in further in vivo studies.
authors.affiliation: College of Pharmacy, Yeungnam University, 214-1, Dae-dong, Gyeongsan 38541, Republic of Korea.
authors.forename: Hanh Thuy
authors.initials: HT
authors.lastname: Nguyen
authors.forename: Raj Kumar
authors.initials: RK
authors.lastname: Thapa
authors.forename: Beom Soo
authors.initials: BS
authors.lastname: Shin
authors.forename: Jee-Heon
authors.initials: JH
authors.lastname: Jeong
authors.forename: Jae-Ryong
authors.initials: JR
authors.lastname: Kim
authors.forename: Chul Soon
authors.initials: CS
authors.lastname: Yong
authors.forename: Jong Oh
authors.initials: JO
authors.lastname: Kim
mesh_terms: D000911:Antibodies, Monoclonal / Q000494:pharmacology*; D002453:Cell Cycle / Q000187:drug effects; D002465:Cell Movement / Q000187:drug effects; D049109:Cell Proliferation / Q000187:drug effects; D016922:Cellular Senescence / Q000187:drug effects*; D020405:Dermis / Q000166:cytology; D005347:Fibroblasts / Q000166:cytology / Q000187:drug effects; D006801:Humans; D008081:Liposomes / Q000648:ultrastructure; D010316:Particle Size; D011092:Polyethylene Glycols / Q000737:chemistry*; D020123:Sirolimus / Q000494:pharmacology*; D060245:Tetraspanin 29 / Q000276:immunology*; D014945:Wound Healing / Q000187:drug effects; D001616:beta-Galactosidase / Q000378:metabolism
chemical_list: D000911:Antibodies, Monoclonal; C554869:CD9 protein, human; D008081:Liposomes; D060245:Tetraspanin 29; D011092:Polyethylene Glycols; D001616:beta-Galactosidase; D020123:Sirolimus