title: Effect of 4-Allyl-1-hydroxy-2-methoxybenzene (Eugenol) on Inflammatory and Apoptosis Processes in Dental Pulp Fibroblasts.
abstract: Eugenol (mixed with zinc oxide powder) is widely used as direct capping material during pulp therapy in primary teeth. The aim of the present study was to evaluate the effect of eugenol on diverse genes involved in inflammatory and cell apoptosis processes. The regulatory effect of eugenol on the expression of inflammation and apoptotic genes was evaluated in dental pulp fibroblasts from extracted third molars, cultured under concentration of eugenol of 13μM. Eugenol allowed the expression of inflammatory and apoptotic genes when compared with positive and negative controls. Eugenol is a proinflammatory agent when it is in direct contact with healthy tissues and behaves as an anti-inflammatory agent in tissues undergoing inflammatory/apoptotic processes, as in cases of pulp inflammation in primary teeth. These findings are relevant for dentistry, when considering the application of safer pulp treatments to grossly carious children's teeth.
authors.affiliation: Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Andrea
authors.initials: A
authors.lastname: Martínez-Herrera
authors.affiliation: Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosí, SLP, Mexico; Basic Sciences Laboratory, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Amaury
authors.identifier: 0000-0003-2314-8465
authors.initials: A
authors.lastname: Pozos-Guillén
authors.affiliation: Basic Sciences Laboratory, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Socorro
authors.initials: S
authors.lastname: Ruiz-Rodríguez
authors.affiliation: Basic Sciences Laboratory, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Arturo
authors.identifier: 0000-0001-9123-0300
authors.initials: A
authors.lastname: Garrocho-Rangel
authors.affiliation: Coordinación Académica Región Altiplano, San Luis Potosi University, 78700 Matehuala, SLP, Mexico.
authors.forename: Antonio
authors.initials: A
authors.lastname: Vértiz-Hernández
authors.affiliation: Pediatric Dentistry Postgraduate Program, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosí, SLP, Mexico; Basic Sciences Laboratory, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosí, SLP, Mexico.
authors.forename: Diana María
authors.identifier: 0000-0002-0089-3414
authors.initials: DM
authors.lastname: Escobar-García
mesh_terms: D000893:Anti-Inflammatory Agents / Q000494:pharmacology; D017209:Apoptosis; D002478:Cells, Cultured; D018076:DNA, Complementary / Q000378:metabolism; D003782:Dental Pulp / Q000166:cytology*; D005054:Eugenol / Q000378:metabolism / Q000627:therapeutic use*; D005347:Fibroblasts / Q000187:drug effects* / Q000378:metabolism; D006801:Humans; D007249:Inflammation / Q000188:drug therapy*; D016328:NF-kappa B / Q000378:metabolism; D014409:Tumor Necrosis Factor-alpha / Q000378:metabolism
chemical_list: D000893:Anti-Inflammatory Agents; D018076:DNA, Complementary; D016328:NF-kappa B; D014409:Tumor Necrosis Factor-alpha; D005054:Eugenol