title: Identification of morphological and biochemical changes in keratin-8/18 knock-down cells using Raman spectroscopy.
abstract: Accurate understanding of cellular processes and responses to stimuli is of paramount importance in biomedical research and diagnosis. Raman spectroscopy (RS), a label-free and nondestructive spectroscopic method has the potential to serve as a novel 'theranostics' tool. Both fiber-optic and micro-Raman studies have demonstrated efficacy in diagnostics and therapeutic response monitoring. In the present study, we have evaluated the potential of micro-Raman spectroscopic maps in identifying changes induced by loss of K8/18 proteins in a tongue cancer cell line. Furthermore, we also evaluated the efficacy of less expensive and commercially available fiber probes to identify K8/18 wild and knock-down cell pellets, in view of the utility of cell pellet-based studies. The findings suggest that major differences in the cellular morphology and biochemical composition can be objectively identified and can be utilized for classification using both micro-Raman and fiber-probe-based RS. These findings highlight the potential of fiber-optic probe-based RS in noninvasive cellular phenotyping for diagnosis and therapeutic response monitoring, especially in low-resource settings.
authors.affiliation: Advanced Centre for Treatment, Research & Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.|Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: S P
authors.initials: SP
authors.lastname: Singh
authors.affiliation: Advanced Centre for Treatment, Research & Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
authors.forename: Hunain
authors.initials: H
authors.lastname: Alam
authors.affiliation: Advanced Centre for Treatment, Research & Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
authors.forename: Crismita
authors.initials: C
authors.lastname: Dmello
authors.affiliation: WITec GmbH, Lisemeitnerstr. 6, 89081, Ulm, Germany.
authors.forename: Hitesh
authors.initials: H
authors.lastname: Mamgain
authors.affiliation: Advanced Centre for Treatment, Research & Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
authors.forename: Milind M
authors.initials: MM
authors.lastname: Vaidya
authors.affiliation: Laser Biomedical Research Center, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
authors.forename: Ramachandra Rao
authors.initials: RR
authors.lastname: Dasari
authors.affiliation: Advanced Centre for Treatment, Research & Education in Cancer (ACTREC), Tata Memorial Centre, Kharghar, Navi Mumbai, 410210, India.
authors.forename: C Murali
authors.initials: CM
authors.lastname: Krishna
mesh_terms: D045744:Cell Line, Tumor; D055785:Gene Knockdown Techniques; D006801:Humans; D053538:Keratin-18 / Q000172:deficiency* / Q000235:genetics*; D053533:Keratin-8 / Q000172:deficiency* / Q000235:genetics*; D009062:Mouth Neoplasms / Q000473:pathology; D013059:Spectrum Analysis, Raman
chemical_list: D053538:Keratin-18; D053533:Keratin-8
keywords: Raman spectroscopy; biophotonics; keratin; oral cancers; wild and single knockdown cells