title: Distribution of Glycoconjugates in the Kidney Studied by Use of Labeled Lectins1.
abstract: Distribution of glycoconjugates in different areas of the rat kidney was studied by light and electron microscopy using six different horseradish peroxidase-labeled lectins. Glomeruli and brush borders of the proximal tubules reacted differently to these lectins, which indicated differences in the carbohydrate compositions of those regions. The ascending limb of Henle's loop (ALH) had strong binding sites for peanut agglutinin (PNA) and soybean agglutinin (SBA). Dolichos biflorus agglutinin (DBA) did not stain the cells of ALH but did stain those of distal convoluted tubules (DCT). DBA is a good marker for distinguishing ALH from DCT. DBA, PNA, and SBA were also good markers of the collecting duct. Ricinus communis agglutinin (RCA-1) and wheat germ agglutinin (WGA) diffusely stained the various components of different parts of the kidney.
authors.affiliation: Departments of Anatomy and Biochemistry, Kagoshima University School of Medicine, Kagoshima 890, Japan (OA 82-268S1).
authors.forename: F
authors.initials: F
authors.lastname: Murata
authors.affiliation: Departments of Anatomy and Biochemistry, Kagoshima University School of Medicine, Kagoshima 890, Japan (OA 82-268S1).
authors.forename: S
authors.initials: S
authors.lastname: Tsuyama
authors.affiliation: Departments of Anatomy and Biochemistry, Kagoshima University School of Medicine, Kagoshima 890, Japan (OA 82-268S1).
authors.forename: S
authors.initials: S
authors.lastname: Suzuki
authors.affiliation: Departments of Anatomy and Biochemistry, Kagoshima University School of Medicine, Kagoshima 890, Japan (OA 82-268S1).
authors.forename: H
authors.initials: H
authors.lastname: Hamada
authors.affiliation: Departments of Anatomy and Biochemistry, Kagoshima University School of Medicine, Kagoshima 890, Japan (OA 82-268S1).
authors.forename: M
authors.initials: M
authors.lastname: Ozawa
authors.affiliation: Departments of Anatomy and Biochemistry, Kagoshima University School of Medicine, Kagoshima 890, Japan (OA 82-268S1).
authors.forename: T
authors.initials: T
authors.lastname: Muramatsu
keywords: Dolichos biflorus agglutinin; Glycoconjugate; Kidney; Peanut agglutinin; Ricinus communis-1 agglutinin; Soybean agglutinin; Ulex europaeus-1 agglutinin; Wheat germ agglutinin.