title: Signal Peptidase Enzymology and Substrate Specificity Profiling.
abstract: Signal peptidases are membrane proteases that play crucial roles in the protein transport pathway of bacteria. They cleave off the signal peptide from precursor proteins that are membrane inserted by the SecYEG or Tat translocons. Signal peptide cleavage releases the translocated protein from the inner membrane allowing the protein to be exported to the periplasm, outer membrane, or secreted into the medium. Signal peptidases are very important proteins to study. They are unique serine proteases with a Ser-Lys dyad, catalyze cleavage at the membrane surface, and are promising potential antibacterial drug targets. This chapter will focus on the isolation of signal peptidases and the preprotein substrates, as well as describe a peptide library approach for characterizing the substrate specificity.
authors.affiliation: The Ohio State University, Columbus, OH, United States. Electronic address: dalbey@chemistry.ohio-state.edu.
authors.forename: R E
authors.initials: RE
authors.lastname: Dalbey
authors.affiliation: The Ohio State University, Columbus, OH, United States.
authors.forename: D
authors.initials: D
authors.lastname: Pei
authors.affiliation: The Ohio State University, Newark, OH, United States.
authors.forename: Ö D
authors.initials: ÖD
authors.lastname: Ekici
mesh_terms: D000595:Amino Acid Sequence / Q000235:genetics; D020134:Catalytic Domain; D008565:Membrane Proteins / Q000737:chemistry* / Q000378:metabolism; D008967:Molecular Biology / Q000379:methods*; D019151:Peptide Library; D019897:Periplasm / Q000737:chemistry / Q000378:metabolism; D011487:Protein Conformation; D021382:Protein Sorting Signals / Q000235:genetics*; D021381:Protein Transport / Q000235:genetics; D017386:Sequence Homology, Amino Acid; D012697:Serine Endopeptidases / Q000737:chemistry* / Q000378:metabolism; D057057:Serine Proteases / Q000737:chemistry* / Q000378:metabolism; D013211:Staphylococcus aureus / Q000201:enzymology; D013379:Substrate Specificity
chemical_list: D008565:Membrane Proteins; D019151:Peptide Library; D021382:Protein Sorting Signals; D057057:Serine Proteases; D012697:Serine Endopeptidases; C026515:type I signal peptidase
keywords: Combinatorial peptide library; Interfacial catalysis; Serine protease; Serine-lysine dyad; Signal peptidase; Substrate specificity