title: Designation of pathogenic resistant bacteria in the Sparusaurata sea collected in Tunisia coastlines: Correlation with high performance liquid chromatography-tandem mass spectrometry analysis of antibiotics.
abstract: Vibrio is characterized by a large number of species and some of them are human pathogens causing gastro intestinal and wound infections through the ingestion or manipulation of contaminated fishes including Vibrio parahaemolyticus and Vibrio alginolyticus. In this study, we reported the phenotypic and molecular characterization of Vibrio parahaemolyticus and Vibrio alginolyticus strains isolated from wild and farm sea bream (Sparus aurata L.) along the Tunisian coast from December 2015 to April 2016. Therefore, the antibiograms indicate a difference between farmed and wild fish. Resistance against amoxicillin antibiotic appears for the bacteria isolated from wild fish, while those from aquaculture farming presented sensitivity to amoxicillin and resistance to antibiotics colistin and fusidic acid. The chloramphenicol antibiotic exhibited a high sensitivity in all isolated bacteria. In fact, traces of amoxicillin in the organs of the fish from Hergla farm were detected by UPLC-MS/MS analysis during December 2016 to April 2016. In addition, antibiotics were detected in January 2014 with high concentration of norfloxacin 2262 ng/g in fish from Hergla coast. The results obtained in this work indicated that the use and presence of antibiotics in water impacts on the occurrence of resistant bacteria and the detection of antibiotic in fish.
authors.affiliation: Research Unit of Analysis and Process Applied to the Environmental - APAE Higher Institute of Applied Sciences and Technology Mahdia - University of Monastir, Tunisia; Laboratory of Chemical Residues and Contaminants, Direction of Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium.
authors.forename: Amina
authors.initials: A
authors.lastname: Zouiten
authors.affiliation: Laboratoire Traitement et recyclage des eaux, Centre de Recherche et Technologie des Eaux, BorjCedria, Tunisia.
authors.forename: Ines
authors.initials: I
authors.lastname: Mehri
authors.affiliation: Research Unit of Analysis and Process Applied to the Environmental - APAE Higher Institute of Applied Sciences and Technology Mahdia - University of Monastir, Tunisia; Laboratory of Chemical Residues and Contaminants, Direction of Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium.
authors.forename: Asma
authors.initials: A
authors.lastname: Beltifa
authors.affiliation: Hygiene Laboratory/Toxicology Unit Hedi CHAKER Hospital, Sfax, Tunisia.
authors.forename: Asma
authors.initials: A
authors.lastname: Ghorbel
authors.affiliation: Université Bretagne Sud, FRE CNRS 3744, IRDL, 56017 Vannes, France.
authors.forename: Olivier
authors.initials: O
authors.lastname: Sire
authors.affiliation: Laboratory of Chemical Residues and Contaminants, Direction of Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium.
authors.forename: Joris
authors.initials: J
authors.lastname: Van Loco
authors.affiliation: Laboratoire Traitement et recyclage des eaux, Centre de Recherche et Technologie des Eaux, BorjCedria, Tunisia.
authors.forename: Hassen
authors.initials: H
authors.lastname: Abdenaceur
authors.affiliation: Laboratory of Chemical Residues and Contaminants, Direction of Food, Medicines and Consumer Safety, Scientific Institute of Public Health, Juliette Wytsmanstraat 14, 1050 Brussels, Belgium.
authors.forename: Tim
authors.initials: T
authors.lastname: Reyns
authors.affiliation: Research Unit of Analysis and Process Applied to the Environmental - APAE Higher Institute of Applied Sciences and Technology Mahdia - University of Monastir, Tunisia. Electronic address: hdbenmansour@gmail.com.
authors.forename: Hedi
authors.initials: H
authors.lastname: Ben Mansour
mesh_terms: D000658:Amoxicillin / Q000494:pharmacology; D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D017756:Aquaculture; D001419:Bacteria / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification; D002701:Chloramphenicol / Q000494:pharmacology; D002851:Chromatography, High Pressure Liquid / Q000379:methods*; D003091:Colistin / Q000494:pharmacology; D004269:DNA, Bacterial; D024901:Drug Resistance, Multiple, Bacterial / Q000187:drug effects; D005393:Fish Diseases / Q000382:microbiology; D005398:Fisheries; D005506:Food Contamination / Q000032:analysis*; D005672:Fusidic Acid / Q000494:pharmacology; D008826:Microbial Sensitivity Tests / Q000379:methods; D009643:Norfloxacin / Q000494:pharmacology; D021541:Sea Bream / Q000382:microbiology*; D017747:Seafood / Q000382:microbiology*; D012623:Seawater / Q000737:chemistry / Q000382:microbiology; D053719:Tandem Mass Spectrometry / Q000379:methods*; D014416:Tunisia; D014735:Vibrio Infections / Q000382:microbiology / Q000662:veterinary*; D044149:Vibrio alginolyticus / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D014736:Vibrio parahaemolyticus / Q000737:chemistry / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D014874:Water Pollutants, Chemical / Q000737:chemistry
chemical_list: D000900:Anti-Bacterial Agents; D004269:DNA, Bacterial; D014874:Water Pollutants, Chemical; D005672:Fusidic Acid; D002701:Chloramphenicol; D000658:Amoxicillin; D009643:Norfloxacin; D003091:Colistin
keywords: Antibiotic pollution; Sparus aurata L.; UPMC-MS/MS; Vibrio alginolyticus; Vibrio parahaemolyticus