title: Aerobic denitrification and biomineralization by a novel heterotrophic bacterium, Acinetobacter sp. H36.
abstract: A novel aerobic denitrification and biomineralization strain H36 was isolated from the Qu Jiang artificial lake. Based on phylogenetic characteristics, the isolated strain was identified as Acinetobacter species. Strain H36 was confirmed to have the ability to perform simultaneous denitrification and biomineralization. Results showed the strain H36 had the capability to completely reduce 96.29% of NO3--N and 78.59% of Ca2+over 112h under aerobic condition. Response surface methodology (RSM) analysis demonstrated the highest removal ratio of Ca2+was 74.24% with hardness concentration of 350mg/L, pH of 8.5, organic concentration of 0.75g/L and inoculum size of 15%. The highest removal ratio of nitrate was 77.00% with hardness concentration of 350mg/L, pH of 7.5, organic concentration of 0.75g/L and inoculum size of 10%. Besides, X-ray diffraction (XRD) analysis showed calcium carbonate could be formed in the process of biomineralization.
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China; State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China. Electronic address: sjf1977518@sina.com.
authors.forename: Jun Feng
authors.initials: JF
authors.lastname: Su
authors.affiliation: School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China.
authors.forename: Jing Xin
authors.initials: JX
authors.lastname: Shi
authors.affiliation: State Key Laboratory of Urban Water Resource and Environment, School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, China.
authors.forename: Fang
authors.initials: F
authors.lastname: Ma
mesh_terms: D000150:Acinetobacter / Q000378:metabolism*; D000332:Aerobiosis; D002113:Calcification, Physiologic; D002119:Calcium Carbonate / Q000378:metabolism; D058440:Denitrification; D052836:Heterotrophic Processes; D058465:Nitrification
chemical_list: D002119:Calcium Carbonate
keywords: Acinetobacter sp.; Biomineralization; Denitrification; Hardness