title: Deletion of admB gene encoding a fungal ADAM affects cell wall construction in Aspergillus oryzae.
abstract: Mammals possess a unique signaling system based on the proteolytic mechanism of a disintegrin and metalloproteinases (ADAMs) on the cell surface. We found two genes encoding ADAMs in Aspergillus oryzae and named them admA and admB. We produced admA and admB deletion strains to elucidate their biological function and clarify whether fungal ADAMs play a similar role as in mammals. The ∆admA∆admB and ∆admB strains were sensitive to cell wall-perturbing agents, congo red, and calcofluor white. Moreover, the two strains showed significantly increased weights of total alkali-soluble fractions from the mycelial cell wall compared to the control strain. Furthermore, ∆admB showed MpkA phosphorylation at lower concentration of congo red stimulation than the control strain. However, the MpkA phosphorylation level was not different between ∆admB and the control strain without the stimulation. The results indicated that A. oryzae AdmB involved in the cell wall integrity without going through the MpkA pathway.
authors.affiliation: a Department of Applied Life Science, The United Graduate School of Agricultural Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Takuji
authors.initials: T
authors.lastname: Kobayashi
authors.affiliation: a Department of Applied Life Science, The United Graduate School of Agricultural Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Hiroshi
authors.initials: H
authors.lastname: Maeda
authors.affiliation: a Department of Applied Life Science, The United Graduate School of Agricultural Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Michio
authors.initials: M
authors.lastname: Takeuchi
authors.affiliation: a Department of Applied Life Science, The United Graduate School of Agricultural Science , Tokyo University of Agriculture and Technology , Fuchu , Japan.
authors.forename: Youhei
authors.initials: Y
authors.lastname: Yamagata
mesh_terms: D051722:ADAM Proteins / Q000172:deficiency* / Q000235:genetics*; D001236:Aspergillus oryzae / Q000166:cytology* / Q000235:genetics* / Q000378:metabolism; D002473:Cell Wall / Q000378:metabolism*; D005656:Fungal Proteins / Q000235:genetics* / Q000378:metabolism; D017353:Gene Deletion; D015966:Gene Expression Regulation, Fungal; D023281:Genomics; D007700:Kinetics; D010766:Phosphorylation; D011134:Polysaccharides / Q000378:metabolism; D014158:Transcription, Genetic
chemical_list: D005656:Fungal Proteins; D011134:Polysaccharides; D051722:ADAM Proteins
keywords: Aspergillus oryzae; a disintegrin and metalloproteinase; cell wall