title: Assessment of IL-18 Serum Level and Its Promoter Polymorphisms in the Saudi Coronary Artery Disease (CAD) Patients.
abstract: The purpose of the current study was to find out the possible changes polymorphic site at the promoter region of IL-18 gene in Saudi CAD patients. We have also measured serum IL-18 level to find out, the likely association between its level and polymorphic site. The present study included total 197 subjects (98 confirmed CAD patients both men and women and 99 healthy control individuals). Serum concentration of IL-18 was measured by enzyme linked immuno-sorbent assay. For SNPs analysis, sanger method of DNA sequencing was followed. We observed variable numbers of SNPs at -137 C/G, -607 A/C, and -656 T/G promoter sites in our studied samples. However, the observed changes in the number of SNP hotspots were found to be non-significant compared with control. IL-18 level was found to be significantly (P < 0.001) elevated in CAD patients compared with control individuals. The highest rise of around 36% (P < 0.001) in IL-18 level was recorded in unstable angina (UA) patients. Moreover, the group belonging to UA and non-ST segment elevation myocardial infarction (NSTEMI) showed only 6% rise. On the basis of our result, inflammation seems to have a role in the pathogenesis of CAD but not leading to the significant changes at the genetic level. J. Cell. Biochem. 118: 1849-1854, 2017. © 2017 Wiley Periodicals, Inc.
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Nasimudeen R
authors.initials: NR
authors.lastname: Jabir
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Chelapram K
authors.initials: CK
authors.lastname: Firoz
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Mohammad A
authors.initials: MA
authors.lastname: Kamal
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Ghazi A
authors.initials: GA
authors.lastname: Damanhouri
authors.affiliation: Faculty of Medicine, Department of Cardiology, King Abdulaziz University Hospital, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Mohammed Nabil
authors.initials: MN
authors.lastname: Alama
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Qamre
authors.initials: Q
authors.lastname: Alam
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Absarul
authors.initials: A
authors.lastname: Haque
authors.affiliation: Faculty of Science, Department of Biology, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Hussein A
authors.initials: HA
authors.lastname: Almehdar
authors.affiliation: King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
authors.forename: Shams
authors.initials: S
authors.lastname: Tabrez
mesh_terms: D000328:Adult; D003324:Coronary Artery Disease / Q000097:blood* / Q000235:genetics*; D005260:Female; D005787:Gene Frequency / Q000235:genetics; D005838:Genotype; D006801:Humans; D020382:Interleukin-18 / Q000097:blood* / Q000235:genetics*; D008297:Male; D008875:Middle Aged; D009203:Myocardial Infarction / Q000097:blood / Q000235:genetics; D020641:Polymorphism, Single Nucleotide / Q000235:genetics; D011401:Promoter Regions, Genetic / Q000235:genetics*; D012529:Saudi Arabia
chemical_list: D020382:Interleukin-18
keywords: CORONARY ARTERY DISEASE; ELISA; IL-18; POLYMORPHISM; SERUM