title: Protective effect of Tritone (Livosone) on oxidative DNA damage and its hepatoprotective potential against various hepatotoxic agent in wistar rats.
abstract: OBJECTIVE
  To evaluate antioxidant activity, DNA damage inhibition and hepatoprotecitve potential of polyherbal formulation Tritone (Livosone).
  
  
  METHODS
  In vitro antioxidant activity of Tritone formulation was performed by using DPPH assay. Hepatoprotecitve potential of Tritone was evaluated against various hepatotoxic agents including Paracetamol (2g/kg b. wt p.o. single dose on 15th day), Galactosamine (400mg/kg b. wt. i.p. single dose on 8th day) and Alcohol (30% p.o.1ml/100g of rat for 15days). Tritone formulation at the doses of (40.5, 81 and 162mg/kg) and standard silymarin (100mg/kg) and Liv52 (270mg/kg) were administered p.o. The hepatoprotective assessment was done by estimating biochemical parameters: SGOT, SGPT, ALP and Total Bilirubin total protein and ChE levels. Additionally histopathological and DNA fragmentation study of Tritone was also performed.
  
  
  RESULTS
  Administration of hepatotoxins (paracetamol, D-GaiN and alcohol) in experimental animals showed significant biochemical, histological deterioration and DNA fragmentation. Pretreatment with Tritone (Livosone) shows significant reduction in serum SGOT, SGPT, ALP and total bilirubin levels and shows significant elevation in total protein and cholinesterase (ChE) levels compared to groups treated with hepatotoxic agents. Histopathological observations of rat liver pretreated with Tritone (Livosone) shows significant protection against hepatic damage. Inhibition of DNA fragmentation by Tritone indicates protective effect of formulation on liver at molecular level. Finally all the results were compared with standard drugs Silymarin and Liv52.
  
  
  CONCLUSIONS
  Correlation of antioxidant activity, biochemical results, histopathological changes and inhibition of DNA damage after treatment with Tritone shows maximum hepatoprotective potential at dose 81mg/kg and 162mg/kg.
authors.affiliation: Department of Pharmacology, Satara College of Pharmacy, Degaon, Satara, MH, India. Electronic address: smedhekar5@gmail.com.
authors.forename: Sheetal Kashinath
authors.initials: SK
authors.lastname: Medhekar
authors.affiliation: R & D center, Nisarga Biotech Pvt Ltd, Satara, MH, India. Electronic address: tejas.nisargabiotech@gmail.com.
authors.forename: Tejas Pandurang
authors.initials: TP
authors.lastname: Jadhav
authors.affiliation: R & D center, Nisarga Biotech Pvt Ltd, Satara, MH, India. Electronic address: vishal.nisargabiotech@gmail.com.
authors.forename: Vishal Sadashiv
authors.initials: VS
authors.lastname: Sasane
authors.affiliation: Department of Pharmacology, Satara College of Pharmacy, Degaon, Satara, MH, India.
authors.forename: Vikas Suresh
authors.initials: VS
authors.lastname: Shende
authors.affiliation: Department of Pharmaceutics, Satara College of Pharmacy, Degaon, Satara, MH, India.
authors.forename: Nagesh Hanmantrao
authors.initials: NH
authors.lastname: Aloorkar
authors.affiliation: Department of Pharmacology, Satara College of Pharmacy, Degaon, Satara, MH, India.
authors.forename: Anjali Baburao
authors.initials: AB
authors.lastname: Chincholkar
authors.affiliation: R & D center, Nisarga Biotech Pvt Ltd, Satara, MH, India.
authors.forename: Girish Sudhakar
authors.initials: GS
authors.lastname: Soman
authors.affiliation: Department of Pharmaceutics, Satara College of Pharmacy, Degaon, Satara, MH, India.
authors.forename: Ajit Shankarrao
authors.initials: AS
authors.lastname: Kulkarni
mesh_terms: D000082:Acetaminophen / Q000633:toxicity; D000818:Animals; D000975:Antioxidants / Q000494:pharmacology*; D004249:DNA Damage / Q000187:drug effects*; D005260:Female; D008099:Liver / Q000187:drug effects*; D008297:Male; D018384:Oxidative Stress / Q000187:drug effects*; D010936:Plant Extracts / Q000494:pharmacology*; D051381:Rats; D017208:Rats, Wistar
chemical_list: D000975:Antioxidants; D010936:Plant Extracts; D000082:Acetaminophen
keywords: Antioxidants; Cholinesterase (ChE); DNA fragmentation; Hepatoprotective; Hepatotoxicity; Tritone (Livosone)