title: Supplementing diet with Manitoba lingonberry juice reduces kidney ischemia-reperfusion injury.
abstract: BACKGROUND
  Lingonberry (Vaccinium vitis-idaea L.) contains high levels of anthocyanins which are bioavailable in the kidney and may be protective against ischemia-reperfusion (IR)-induced acute kidney injury. This study investigated the effect of lingonberry juice on the IR-induced stress-activated signalling pathway and inflammatory response in the kidney.
  
  
  RESULTS
  Sprague-Dawley rats subjected to kidney IR had significantly impaired kidney function, with increased activation of the JNK signalling pathway and increased inflammatory response, measured using a multiplex panel containing an extensive array of inflammatory biomarkers. In rats fed 1 mL lingonberry juice daily for 3 weeks prior to IR, kidney function was protected and attenuation of inflammatory response and JNK signalling was reflected in the reduction of the measured biomarkers. In vitro results in cultured HK-2 cells confirmed that lingonberry anthocyanins reduced JNK signalling and inflammatory gene expression after IR.
  
  
  CONCLUSIONS
  This study shows, for the first time, that daily supplementation with lingonberry juice may protect against loss of kidney function induced by IR injury by modulating JNK signalling and inhibiting the subsequent inflammatory response. © 2017 Her Majesty the Queen in Right of Canada. Journal of the Science of Food and Agriculture © 2017 Society of Chemical Industry.
authors.affiliation: Agriculture and Agri-Food Canada, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Department of Physiology & Pathophysiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Cara K
authors.initials: CK
authors.lastname: Isaak
authors.affiliation: Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Pengqi
authors.initials: P
authors.lastname: Wang
authors.affiliation: Agriculture and Agri-Food Canada, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.
authors.forename: Suvira
authors.initials: S
authors.lastname: Prashar
authors.affiliation: Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Department of Physiology & Pathophysiology, University of Manitoba, Winnipeg, Manitoba, Canada.|Department of Animal Science, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Karmin
authors.initials: K
authors.lastname: O
authors.affiliation: Agriculture and Agri-Food Canada, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Department of Human Nutritional Science, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Daniel Cw
authors.initials: DC
authors.lastname: Brown
authors.affiliation: Agriculture and Agri-Food Canada, St John's Research and Development Centre, St John's, Newfoundland and Labrador, Canada.
authors.forename: Samir C
authors.initials: SC
authors.lastname: Debnath
authors.affiliation: Agriculture and Agri-Food Canada, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Canadian Centre for Agri-Food Research in Health and Medicine, St Boniface Hospital Albrechtsen Research Centre, Winnipeg, Manitoba, Canada.|Department of Physiology & Pathophysiology, University of Manitoba, Winnipeg, Manitoba, Canada.
authors.forename: Yaw L
authors.identifier: http://orcid.org/0000-0001-6623-2881
authors.initials: YL
authors.lastname: Siow
mesh_terms: D000818:Animals; D015415:Biomarkers / Q000378:metabolism; D000067030:Fruit and Vegetable Juices / Q000032:analysis*; D006801:Humans; D007668:Kidney / Q000378:metabolism / Q000503:physiopathology; D007674:Kidney Diseases / Q000178:diet therapy* / Q000209:etiology / Q000378:metabolism / Q000503:physiopathology; D008297:Male; D008350:Manitoba; D051381:Rats; D017207:Rats, Sprague-Dawley; D015427:Reperfusion Injury / Q000150:complications*; D015398:Signal Transduction; D029801:Vaccinium vitis-idaea / Q000737:chemistry / Q000378:metabolism*
chemical_list: D015415:Biomarkers
keywords: Vaccinium vitis-idaea; anthocyanins; biomarkers; ischemia-reperfusion; kidney injury; lingonberry