title: [Application of rabbit monoclonal antibody GCET2 in diagnosis of diffuse large B cell lymphoma].
abstract: Objective:To prepare a rabbit monoclonal antibody GCET2 and to investigate its diagnostic value in the workup of diffuse large B-cell lymphoma (DLBCL).Methods:GCET2 rabbit monoclonal antibody was developed by using RabMAb®technology, and its specificity was confirmed by ELISA, Western blot, immunohistochemistry (IHC) and flow cytometry. A panel of immunomarkers including GCET2, CD10, bcl-6, MUM1, GCET1, FOXP1, Ki-67 and CMYC was evaluated in 81 cases of DLBCLs, 5 cases of follicular lymphomas (FL) and 2 cases of Burkitt's lymphomas.Results:Rabbit monoclonal GCET2 antibody (clone EP316) was developed with specificity for normal germinal center B-cells (GCB) and GCB origined lymphomas. In 81 cases of DLBCL, the positive rate of GCET2 was 43.2%(35/81), which was significantly higher than that of other germinal center markers. Moreover, among 81 DLBCLs, the proportions of high CMYC expression in GCET2 negative and positive groups were 15.2% (7/46) and 2.8% (1/35), respectively.Conclusion:GCET2 is a sensitive immunohistochemical marker for GCB derived lymphomas. Combined with other biomarkers, it may improve the diagnostic sensitivity of GCB-DLBCL.
authors.affiliation: Department of Pathology and Pathophysiology, Institute of Pathology and Forensic Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China.
authors.forename: H Y
authors.initials: HY
authors.lastname: Pan
authors.forename: X X
authors.initials: XX
authors.lastname: Zhang
authors.forename: Y Y
authors.initials: YY
authors.lastname: Weng
authors.forename: R
authors.initials: R
authors.lastname: Zhou
mesh_terms: D000911:Antibodies, Monoclonal; D000918:Antibody Specificity; D015415:Biomarkers / Q000032:analysis; D002051:Burkitt Lymphoma / Q000175:diagnosis / Q000276:immunology; D005260:Female; D018858:Germinal Center; D006801:Humans; D007150:Immunohistochemistry; D047908:Intracellular Signaling Peptides and Proteins; D008224:Lymphoma, Follicular / Q000175:diagnosis; D016403:Lymphoma, Large B-Cell, Diffuse / Q000175:diagnosis*; D008297:Male; D008840:Microfilament Proteins; D009363:Neoplasm Proteins / Q000032:analysis* / Q000276:immunology; D051560:Proto-Oncogene Proteins c-bcl-6 / Q000032:analysis; D012680:Sensitivity and Specificity
chemical_list: D000911:Antibodies, Monoclonal; D015415:Biomarkers; C476824:GCSAM protein, human; D047908:Intracellular Signaling Peptides and Proteins; D008840:Microfilament Proteins; D009363:Neoplasm Proteins; D051560:Proto-Oncogene Proteins c-bcl-6