title: Fluorescent Gold Nanoclusters for Selective Detection of Dopamine in Cerebrospinal fluid.
abstract: Since the last two decades, protein conjugated fluorescent gold nanoclusters (NCs) owe much attention in the field of medical and nanobiotechnology due to their excellent photo stability characteristics. In this paper, we reported stable, nontoxic and red fluorescent emission BSA-Au NCs for selective detection of L-dopamine (DA) in cerebrospinal fluid (CSF). The evolution was probed by various instrumental techniques such as UV-vis spectroscopy, High resolution transmission electron microscopy (HTEM), X-ray photoelectron spectroscopy (XPS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), photoluminescence spectroscopy (PL). The synthesised BSA-Au NCs were showing 4-6 nm with high fluorescent ~8% Quantum yield (QY). The fluorescence intensity of BSA-Au NCs was quenched upon the addition of various concentrations of DA via an electron transfer mechanism. The decrease in BSA-Au NCs fluorescence intensity made it possible to determine DA in PBS buffer and the spiked DA in CSF in the linear range from 0 to 10 nM with the limit of detection (LOD) 0.622 and 0.830 nM respectively. Best of our knowledge, as-prepared BSA-Au NCs will gain possible strategy and good platform for biosensor, drug discovery, and rapid disease diagnosis such as Parkinson's and Alzheimer diseases.
authors.affiliation: Department of Bionanotechnology, Gachon University, Gyeonggi-do, 13120, Republic of Korea.
authors.forename: Saravanan
authors.initials: S
authors.lastname: Govindaraju
authors.affiliation: Department of Chemical and Biological Engineering, Gachon University, Gyeonggi-do, 13120, Republic of Korea.
authors.forename: Seshadri Reddy
authors.initials: SR
authors.lastname: Ankireddy
authors.affiliation: Department of Bionanotechnology, Gachon University, Gyeonggi-do, 13120, Republic of Korea.
authors.forename: Buddolla
authors.initials: B
authors.lastname: Viswanath
authors.affiliation: Department of Chemical and Biological Engineering, Gachon University, Gyeonggi-do, 13120, Republic of Korea.
authors.forename: Jongsung
authors.initials: J
authors.lastname: Kim
authors.affiliation: Department of Bionanotechnology, Gachon University, Gyeonggi-do, 13120, Republic of Korea.
authors.forename: Kyusik
authors.initials: K
authors.lastname: Yun
mesh_terms: D004298:Dopamine / Q000134:cerebrospinal fluid*; D005453:Fluorescence; D006046:Gold / Q000737:chemistry*; D053768:Metal Nanoparticles / Q000737:chemistry* / Q000648:ultrastructure; D056951:Photoelectron Spectroscopy; D012710:Serum Albumin, Bovine / Q000737:chemistry; D013056:Spectrophotometry, Ultraviolet; D017550:Spectroscopy, Fourier Transform Infrared; D013997:Time Factors; D014961:X-Ray Diffraction
chemical_list: D012710:Serum Albumin, Bovine; D006046:Gold; D004298:Dopamine