title: Enzymatic antioxidant system of endotheliocytes.
abstract: It is shown that endothelial cells from human umbilical vein have a reduced activity and gene expression of the "classic" antioxidant enzymes (Cu,Zn-superoxide dismutase, catalase, and Se-containing glutathione peroxidase). At the same time, a high expression level of peroxiredoxin genes was identified in the same endothelial cells, which obviously indicates the predominant involvement of these enzymes in protecting the endothelium from the damaging effect of free radical peroxidation.
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: M G
authors.initials: MG
authors.lastname: Sharapov
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: R G
authors.initials: RG
authors.lastname: Goncharov
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: A E
authors.initials: AE
authors.lastname: Gordeeva
authors.affiliation: Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142290, Russia.
authors.forename: V I
authors.initials: VI
authors.lastname: Novoselov
authors.affiliation: Russian Cardiology Research and Production Center, Russian Federation Ministry of Health, 3ya Cherepkovskaya ul. 15A, Moscow, 121552, Russia.
authors.forename: O A
authors.initials: OA
authors.lastname: Antonova
authors.affiliation: Russian Cardiology Research and Production Center, Russian Federation Ministry of Health, 3ya Cherepkovskaya ul. 15A, Moscow, 121552, Russia.
authors.forename: A K
authors.initials: AK
authors.lastname: Tikhaze
authors.affiliation: Russian Cardiology Research and Production Center, Russian Federation Ministry of Health, 3ya Cherepkovskaya ul. 15A, Moscow, 121552, Russia. lankin@cardio.ru.
authors.forename: V Z
authors.initials: VZ
authors.lastname: Lankin
mesh_terms: D002374:Catalase / Q000378:metabolism*; D002478:Cells, Cultured; D042783:Endothelial Cells / Q000201:enzymology*; D004912:Erythrocytes / Q000201:enzymology*; D015870:Gene Expression; D005979:Glutathione Peroxidase / Q000378:metabolism*; D006801:Humans; D060888:Real-Time Polymerase Chain Reaction; D020133:Reverse Transcriptase Polymerase Chain Reaction; D000072105:Superoxide Dismutase-1 / Q000378:metabolism*; D014471:Umbilical Veins / Q000166:cytology / Q000201:enzymology*
chemical_list: D002374:Catalase; D005979:Glutathione Peroxidase; D000072105:Superoxide Dismutase-1