title: Microelectrode array (MEA) platform as a sensitive tool to detect and evaluate Ostreopsis cf. ovata toxicity.
abstract: In the last decade, the occurrence of harmful dinoflagellate blooms of the genus Ostreopsis has increased both in frequency and in geographic distribution with adverse impacts on public health and the economy. Ostreopsis species are producers of palytoxin-like toxins (putative palytoxin and ovatoxins) which are among the most potent natural non-protein compounds known to date, exhibiting extreme toxicity in mammals, including humans. Most existing toxicological data are derived from in vivo mouse assay and are related to acute effects of pure palytoxin, without considering that the toxicity mechanism of dinoflagellates can be dependent on the varying composition of complex biotoxins mixture and on the presence of cellular components. In this study, in vitro neuronal networks coupled to microelectrode array (MEA)-based system are proposed, for the first time, as sensitive biosensors for the evaluation of marine alga toxicity on mammalian cells. Toxic effect was investigated by testing three different treatments of laboratory cultured Ostreopsis cf. ovata cells: filtered and re-suspended algal cells; filtered, re-suspended and sonicated algal cells; conditioned growth medium devoid of algal cells. The great sensitivity of this system revealed the mixture of PTLX-complex analogues naturally released in the growth medium and the different potency of the three treatments to inhibit the neuronal network spontaneous electrical activity. Moreover, by means of the multiparametric analysis of neuronal network activity, the approach revealed a different toxicity mechanism of the cellular component compared to the algal conditioned growth medium, highlighting the potential active role of the first treatment.
authors.affiliation: ETT S.p.A., via Sestri 37, Genoa 16154, Italy; CNR-Institute of Biophysics (IBF), Via De Marini 6, 16149 Genoa, Italy. Electronic address: susanna.alloisio@ettsolutions.com.
authors.forename: Susanna
authors.initials: S
authors.lastname: Alloisio
authors.affiliation: DiSTAV, University of Genoa, Corso Europa 26, 16132 Genoa, Italy.
authors.forename: Valentina
authors.initials: V
authors.lastname: Giussani
authors.affiliation: CNR-Institute of Biophysics (IBF), Via De Marini 6, 16149 Genoa, Italy.
authors.forename: Mario
authors.initials: M
authors.lastname: Nobile
authors.affiliation: DiSTAV, University of Genoa, Corso Europa 26, 16132 Genoa, Italy.
authors.forename: Mariachiara
authors.initials: M
authors.lastname: Chiantore
authors.affiliation: ETT S.p.A., via Sestri 37, Genoa 16154, Italy; CNR-Institute of Biophysics (IBF), Via De Marini 6, 16149 Genoa, Italy.
authors.forename: Antonio
authors.initials: A
authors.lastname: Novellino
mesh_terms: D000818:Animals; D002470:Cell Survival / Q000187:drug effects; D002478:Cells, Cultured; D004141:Dinoflagellida / Q000737:chemistry*; D008387:Marine Toxins / Q000633:toxicity*; D051379:Mice; D008839:Microelectrodes; D018675:Toxicity Tests / Q000379:methods*
chemical_list: D008387:Marine Toxins
keywords: MEA-based testing assay; Ostreopsis cf. ovata; Toxins mixture