title: Close Correlation of Monoamine Oxidase Activity with Progress of Alzheimer's Disease in Mice, Observed byin VivoTwo-Photon Imaging.
abstract: Monoamine oxidases (MAOs) play an important role in Alzheimer's disease (AD) pathology. We reportin vivocomonitoring of MAO activity and amyloid-β (Aβ) plaques dependent on the aging of live mice with AD, using a two-photon fluorescence probe. The probe under the catalytic action of MAO produces a dipolar fluorophore that senses Aβ plaques, a general AD biomarker, enabling us to comonitor the enzyme activity and the progress of AD indicated by Aβ plaques. The results show that the progress of AD has a close correlation with MAO activity, which can be categorized into three stages: slow initiation stage up to three months, an aggressive stage, and a saturation stage from nine months. Histological analysis also reveals elevation of MAO activity around Aβ plaques in aged mice. The close correlation between the MAO activity and AD progress observed byin vivomonitoring for the first time prompts us to investigate the enzyme as a potential biomarker of AD.
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 37673, Republic of Korea; Department of Chemistry and Biochemistry, University of California, San Diego, La Jolla, California 92093, United States.
authors.forename: Dokyoung
authors.initials: D
authors.lastname: Kim
authors.affiliation: Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University , 103 Daehak-Ro, Jongro-Gu, Seoul 110-799, Republic of Korea.
authors.forename: Sung Hoon
authors.initials: SH
authors.lastname: Baik
authors.affiliation: Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University , 103 Daehak-Ro, Jongro-Gu, Seoul 110-799, Republic of Korea.
authors.forename: Seokjo
authors.initials: S
authors.lastname: Kang
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 37673, Republic of Korea.
authors.forename: Seo Won
authors.initials: SW
authors.lastname: Cho
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 37673, Republic of Korea.
authors.forename: Juryang
authors.initials: J
authors.lastname: Bae
authors.affiliation: Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University , 103 Daehak-Ro, Jongro-Gu, Seoul 110-799, Republic of Korea.
authors.forename: Moon-Yong
authors.initials: MY
authors.lastname: Cha
authors.affiliation: Department of Chemistry and Biochemistry, University of California, San Diego , La Jolla, California 92093, United States.
authors.forename: Michael J
authors.initials: MJ
authors.lastname: Sailor
authors.affiliation: Department of Biochemistry and Biomedical Sciences, College of Medicine, Seoul National University , 103 Daehak-Ro, Jongro-Gu, Seoul 110-799, Republic of Korea.
authors.forename: Inhee
authors.initials: I
authors.lastname: Mook-Jung
authors.affiliation: Department of Chemistry, Pohang University of Science and Technology (POSTECH) , 77 Cheongam-Ro, Nam-Gu, Pohang, Gyungbuk 37673, Republic of Korea.
authors.forename: Kyo Han
authors.initials: KH
authors.lastname: Ahn