title: [Serotype identification and antibiotic susceptibility of Shiga toxin-producing Escherichia coli in the Weishan area in Shandong Province, China].
abstract: Objective:To determine the serotypes and drug resistance profiles of Shiga toxin-producingEscherichia coli(STEC) in animal stools from the Weishan area in Shandong Province, China. To provide the basis for further study.Methods:Five hundred animal stool samples (from pigs, cattle, sheep, dogs and birds) were collected from the Weishan area and STEC strains were isolated from these samples. Strains were serotyped by a serum agglutination test, and their drug resistance profiles were determined through antimicrobial sensitivity experiments. In this study, PCR was used to detect tetracycline resistance genes (tetA,tetB,tetC,tetD) and beta-lactam resistance genes (blaSHV-1,blaCTX-M,blaTEM).Results:Sixteen strains of STEC were isolated from animal stool samples. Thirteen strains were isolated from pig stool samples, two from bovine stool samples and one from a sheep stool sample. Two of the strains were identified asE. coliO157:H7, and other 14 strains were non-O157 STEC of different serotypes. Antimicrobial sensitivity experiments showed that 15 of the strains were multidrug resistant. The rates of resistance were as follows: nalidixic acid (12/16 strains), sulfisoxazole (11/16), trimethoprim and sulphame-thoxazole (11/16), doxycycline (9/16), azithromycin (9/16), tetracycline (9/16), chloramphenicol (8/16) and streptomycin (8/16). Therefore, nalidixic acid showed the highest rate of resistance among the strains, followed by trimethoprim and sulphame-thoxazole, and sulfisoxazole. Resistance to cefepime or imipenem was not detected. In total, three types of drug resistance genes (tetA,tetBandtetC) were detected among the 16 strains.Conclusion:The results showed that STEC strains isolated from animals in the Weishan area were of a range of serotypes. The 16 strains of STEC isolated from animal stools in this area were resistant to a number of antibiotics, with many strains displaying multidrug resistance.
authors.affiliation: Department of Epidemiology and Health Statistics, School of Public Health, Shandong University, Jinan 250012, China.
authors.forename: C C
authors.initials: CC
authors.lastname: Shao
authors.forename: B
authors.initials: B
authors.lastname: Hu
authors.forename: Z W
authors.initials: ZW
authors.lastname: Bi
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Kou
authors.forename: M
authors.initials: M
authors.lastname: Fang
authors.forename: B L
authors.initials: BL
authors.lastname: Chen
authors.forename: Z Q
authors.initials: ZQ
authors.lastname: Bi
mesh_terms: D000818:Animals; D000900:Anti-Bacterial Agents / Q000494:pharmacology*; D002417:Cattle; D002681:China; D004285:Dogs; D024901:Drug Resistance, Multiple, Bacterial / Q000235:genetics*; D004926:Escherichia coli; D004927:Escherichia coli Infections / Q000382:microbiology / Q000662:veterinary*; D019453:Escherichia coli O157 / Q000187:drug effects / Q000235:genetics / Q000302:isolation & purification; D029968:Escherichia coli Proteins / Q000378:metabolism; D005243:Feces / Q000382:microbiology*; D008826:Microbial Sensitivity Tests; D010641:Phenotype; D016133:Polymerase Chain Reaction; D065288:Serogroup; D012756:Sheep; D022603:Shiga Toxins / Q000145:classification / Q000235:genetics*; D054323:Shiga-Toxigenic Escherichia coli / Q000145:classification / Q000187:drug effects* / Q000235:genetics / Q000302:isolation & purification*; D013552:Swine
chemical_list: D000900:Anti-Bacterial Agents; D029968:Escherichia coli Proteins; D022603:Shiga Toxins