title: Scriptaid Upregulates Expression of Development-Related Genes, Inhibits Apoptosis, and Improves the Development of Somatic Cell Nuclear Transfer Mini-Pig Embryos.
abstract: The present study was undertaken to investigate the mechanisms by which Scriptaid treatment improves the developmental competence of somatic cell nuclear transfer (SCNT) mini-pig embryos in vitro. We found that treatment with 500 nmol/L Scriptaid for 15 hours significantly improved the development of mini-pig SCNT embryos. Compared with the control group, the blastocyst rate was higher (18.3% vs. 10.7%; p < 0.05). The acetylation level on H3K14 of the Scriptaid-treated group was higher compared with the control group in SCNT embryos at two-cell, four-cell, and blastocyst stages (p < 0.05). After Scriptaid treatment, histone deacetylase gene HDAC5 expression level was significantly decreased in four-cell embryos and blastocysts, while the expression levels of the embryos' development-related genes AKT, Oct4, and apoptosis inhibited gene PGC-1α were significantly increased in blastocysts (p < 0.05). The number of apoptotic cells per blastocyst in the Scriptaid-treated group was lower compared with the control group (p < 0.05). These results indicate that Scriptaid repressed HDCA5 gene expression, increased the acetylation level of H3K14, upregulated the expression of AKT, Oct4, and PGC-1α genes, improved embryos' development, and reduced apoptosis, which favors development of the SCNT mini-pig embryos to blastocysts.
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Li
authors.initials: L
authors.lastname: Zhang
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Yuemeng
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Yanjun
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Jinglei
authors.initials: J
authors.lastname: Si
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Yanna
authors.initials: Y
authors.lastname: Huang
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Qinyang
authors.initials: Q
authors.lastname: Jiang
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Ganqiu
authors.initials: G
authors.lastname: Lan
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Yafen
authors.initials: Y
authors.lastname: Guo
authors.affiliation: College of Animal Science and Technology, Guangxi University , Nanning, China .
authors.forename: Hesheng
authors.initials: H
authors.lastname: Jiang
mesh_terms: D000107:Acetylation; D000818:Animals; D017209:Apoptosis / Q000187:drug effects*; D001755:Blastocyst / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D004622:Embryo, Mammalian / Q000166:cytology* / Q000187:drug effects / Q000378:metabolism; D047108:Embryonic Development / Q000187:drug effects*; D005260:Female; D018507:Gene Expression Regulation, Developmental / Q000187:drug effects*; D050437:Genes, Developmental; D056572:Histone Deacetylase Inhibitors / Q000494:pharmacology; D006657:Histones; D006898:Hydroxylamines / Q000494:pharmacology*; D053652:Nuclear Transfer Techniques / Q000662:veterinary*; D011804:Quinolines / Q000494:pharmacology*; D013552:Swine; D013556:Swine, Miniature; D015533:Transcriptional Activation
chemical_list: D056572:Histone Deacetylase Inhibitors; D006657:Histones; D006898:Hydroxylamines; D011804:Quinolines; C410733:scriptaid
keywords: Scriptaid; cell apoptosis; histone acetylation; metabolic gene; mini-pig; somatic cell nuclear transfer