title: Mesenchymal stroma cells in peritoneal dialysis effluents from patients.
abstract: Mesenchymal stroma cells (MSCs) have potential as an emerging cell therapy for treating many different diseases, but discovery of the practical sources of MSCs is needed for the large-scale clinical application of this therapy. This study was to identify MSCs in peritoneal dialysis (PD) effluents that were discarded after PD. The effluents were collected from patients who were on the dialysis for less than 1 month. Adherent cells from the effluents were isolated by incubation in serum-containing medium in plastic culture dishes. Cell surface markers were determined by a flow cytometric analysis, and the in vitro differentiation to chondrocytes, osteocytes or adipocytes was confirmed by staining with a specific dye. After four passages, these isolated cells displayed the typical morphology of mesenchymal cells in traditional 2-D cultures, and were grown to form spherical colonies in 3-D collagen cultures. Flow cytometric analysis revealed that the unsorted cells from all of seven patient samples showed robust expression of typical mesenchymal marker CD29, CD44, CD73, CD90 and CD166, and the absence of CD34, CD79a, CD105, CD271, SSEA-4, Stro-1 and HLA-DR. In differentiation assays, these cells were induced in vitro to chondrocytes, osteocytes or adipocytes. In conclusion, this preliminary study suggests the presence of MSCs in the "discarded" PD effluents. Further characterization of the phenotypes of these MSCs and evaluation of their therapeutic potential, particularly for the prevention of PD failure, are needed.
authors.affiliation: Department of Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin, 300052, China.|Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Bin
authors.initials: B
authors.lastname: Liu
authors.affiliation: Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.
authors.forename: Qiunong
authors.initials: Q
authors.lastname: Guan
authors.affiliation: Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada.|Department of Anesthesiology, General Hospital of Tianjin Medical University, Tianjin, 300052, China.
authors.forename: Jing
authors.initials: J
authors.lastname: Li
authors.affiliation: Fraser Health Nephrology, Royal Columbian Hospital, New Westminster, BC, Canada.
authors.forename: Gerald
authors.initials: G
authors.lastname: da Roza
authors.affiliation: Department of General Surgery, Tianjin Medical University General Hospital,154 Anshan Road, Heping District, Tianjin, 300052, China. hwangca272@hotmail.com.
authors.forename: Hao
authors.initials: H
authors.lastname: Wang
authors.affiliation: Department of Urologic Sciences, University of British Columbia, Vancouver, BC, Canada. caigan@mail.ubc.ca.|Jack Bell Research Centre, Room 250A, 2660 Oak Street, Vancouver, BC, V6H 3Z6, Canada. caigan@mail.ubc.ca.
authors.forename: Caigan
authors.initials: C
authors.lastname: Du
mesh_terms: D017667:Adipocytes; D000368:Aged; D015703:Antigens, CD / Q000032:analysis; D001202:Ascitic Fluid / Q000166:cytology*; D002454:Cell Differentiation; D002469:Cell Separation; D064987:Cell- and Tissue-Based Therapy; D002478:Cells, Cultured; D019902:Chondrocytes; D005260:Female; D006801:Humans; D008297:Male; D059630:Mesenchymal Stem Cells / Q000166:cytology / Q000378:metabolism; D008875:Middle Aged; D010011:Osteocytes; D010530:Peritoneal Dialysis
chemical_list: D015703:Antigens, CD
keywords: Peritoneal cavity; Peritoneal dialysis effluent; Peritoneal mesenchymal stroma cells