title: Aspirin and (or) omega-3 polyunsaturated fatty acids protect against corticohippocampal neurodegeneration and downregulate lipoxin A4production and formyl peptide receptor-like 1 expression in pentylenetetrazole-kindled rats.
abstract: There is evidence for a relationship between inflammation and seizures because epilepsy can be caused by or result in inflammation. This study aimed to investigate the effect of aspirin and (or) omega-3 polyunsaturated fatty acids (PUFAs) on seizure activity and neurodegeneration in pentylenetetrazole (PTZ)-kindled rats focusing on their effect on corticohippocampal production of lipoxin A4(LXA4) and expression of formyl peptide receptor-like 1 (FPRL1) receptors. Male rats were injected with PTZ (35 mg/kg, i.p.) 3 times per week for a total of 15 doses. Rats were treated daily with aspirin (20 mg/kg, i.p.), omega-3 PUFAs (85 mg/kg, p.o.), or a combination of them for 35 days. Both LXA4level and expression of FPRL1 receptor in the cortices and hippocampi of rats' brains were greater in PTZ-kindled rats compared to a saline control group. Cotreatment with aspirin and (or) omega-3 PUFAs reduced convulsive behaviour; reduced levels of LXA4, interleukin-1β, and nuclear factor-κB; and showed a lower percentage of corticohippocampal degenerative cells compared to PTZ-kindled rats. The combination of the 2 therapeutic agents did not provide significant improvement in comparison with the monotherapies. These findings suggest the use of aspirin or omega-3 PUFAs may delay the development of seizures and provide neuroprotection in a clinical setting.
authors.affiliation: a Ismailia General Hospital, Ismailia, Egypt.
authors.forename: Basma A
authors.initials: BA
authors.lastname: Abd-Elghafour
authors.affiliation: b Department of Pharmacology & Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Norhan M
authors.initials: NM
authors.lastname: El-Sayed
authors.affiliation: c Department of Cytology & Histology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, Egypt.
authors.forename: Amal A M
authors.initials: AA
authors.lastname: Ahmed
authors.affiliation: b Department of Pharmacology & Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Sawsan A
authors.initials: SA
authors.lastname: Zaitone
authors.affiliation: b Department of Pharmacology & Toxicology, Faculty of Pharmacy, Suez Canal University, Ismailia 41522, Egypt.
authors.forename: Yasser M
authors.initials: YM
authors.lastname: Moustafa
mesh_terms: D000818:Animals; D001241:Aspirin / Q000627:therapeutic use*; D002540:Cerebral Cortex / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D004195:Disease Models, Animal; D015536:Down-Regulation; D004359:Drug Therapy, Combination; D004827:Epilepsy / Q000139:chemically induced / Q000188:drug therapy / Q000517:prevention & control*; D015525:Fatty Acids, Omega-3 / Q000627:therapeutic use*; D006624:Hippocampus / Q000187:drug effects / Q000378:metabolism / Q000473:pathology; D053583:Interleukin-1beta / Q000378:metabolism; D044045:Lipoxins / Q000378:metabolism*; D008297:Male; D016328:NF-kappa B / Q000378:metabolism; D009410:Nerve Degeneration / Q000517:prevention & control*; D018696:Neuroprotective Agents / Q000627:therapeutic use*; D010433:Pentylenetetrazole / Q000633:toxicity; D051381:Rats; D044042:Receptors, Formyl Peptide / Q000378:metabolism; D044067:Receptors, Lipoxin / Q000378:metabolism*
chemical_list: D015525:Fatty Acids, Omega-3; D053583:Interleukin-1beta; D044045:Lipoxins; D016328:NF-kappa B; D018696:Neuroprotective Agents; D044042:Receptors, Formyl Peptide; D044067:Receptors, Lipoxin; C482540:lipoxin A(4) receptor, rat; C040527:lipoxin A4; D001241:Aspirin; D010433:Pentylenetetrazole
keywords: acides gras polyinsaturés de type ω3; aspirin; aspirine; lipoxin A4; lipoxine A4; omega-3 polyunsaturated fatty acids; pentylenetetrazole; pentylène tétrazole; rat