title: Generation and Characterization of HIV-1 Transmitted and Founder Virus Consensus Sequence from Intravenous Drug Users in Xinjiang, China.
abstract: HIV-1 transmission in intravenous drug users (IDUs) has been characterized by high genetic multiplicity and suggests a greater challenge for HIV-1 infection blocking. We investigated a total of 749 sequences of full-length gp160 gene obtained by single genome sequencing (SGS) from 22 HIV-1 early infected IDUs in Xinjiang province, northwest China, and generated a transmitted and founder virus (T/F virus) consensus sequence (IDU.CON). The T/F virus was classified as subtype CRF07_BC and predicted to be CCR5-tropic virus. The variable region (V1, V2, and V4 loop) of IDU.CON showed length variation compared with the heterosexual T/F virus consensus sequence (HSX.CON) and homosexual T/F virus consensus sequence (MSM.CON). A total of 26 N-linked glycosylation sites were discovered in the IDU.CON sequence, which is less than that of MSM.CON and HSX.CON. Characterization of T/F virus from IDUs highlights the genetic make-up and complexity of virus near the moment of transmission or in early infection preceding systemic dissemination and is important toward the development of an effective HIV-1 preventive methods, including vaccines.
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China .
authors.forename: Fan
authors.initials: F
authors.lastname: Li
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China .
authors.forename: Liying
authors.initials: L
authors.lastname: Ma
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China .
authors.forename: Yi
authors.initials: Y
authors.lastname: Feng
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China .
authors.forename: Jing
authors.initials: J
authors.lastname: Hu
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China .
authors.forename: Na
authors.initials: N
authors.lastname: Ni
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China .
authors.forename: Yuhua
authors.initials: Y
authors.lastname: Ruan
authors.affiliation: 1 State Key Laboratory for Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China .|2 Center of Infectious Diseases, Peking University, Beijing, China .|3 Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hanzhou, China .
authors.forename: Yiming
authors.initials: Y
authors.lastname: Shao
mesh_terms: D002681:China; D016384:Consensus Sequence; D005838:Genotype; D019302:HIV Envelope Protein gp160 / Q000235:genetics; D015658:HIV Infections / Q000635:transmission* / Q000821:virology*; D015497:HIV-1 / Q000145:classification / Q000235:genetics* / Q000302:isolation & purification*; D006801:Humans; D012367:RNA, Viral / Q000235:genetics*; D015819:Substance Abuse, Intravenous / Q000150:complications*
chemical_list: D019302:HIV Envelope Protein gp160; D012367:RNA, Viral
keywords: CRF07_BC; consensus sequence; early infection; intravenous drug users