title: Targeting of the Fun30 nucleosome remodeller by the Dpb11 scaffold facilitates cell cycle-regulated DNA end resection.
abstract: DNA double strand breaks (DSBs) can be repaired by either recombination-based or direct ligation-based mechanisms. Pathway choice is made at the level of DNA end resection, a nucleolytic processing step, which primes DSBs for repair by recombination. Resection is thus under cell cycle control, but additionally regulated by chromatin and nucleosome remodellers. Here, we show that both layers of control converge in the regulation of resection by the evolutionarily conserved Fun30/SMARCAD1 remodeller. Budding yeast Fun30 and human SMARCAD1 are cell cycle-regulated by interaction with the DSB-localized scaffold protein Dpb11/TOPBP1, respectively. In yeast, this protein assembly additionally comprises the 9-1-1 damage sensor, is involved in localizing Fun30 to damaged chromatin, and thus is required for efficient long-range resection of DSBs. Notably, artificial targeting of Fun30 to DSBs is sufficient to bypass the cell cycle regulation of long-range resection, indicating that chromatin remodelling during resection is underlying DSB repair pathway choice.
authors.affiliation: DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Susanne Cs
authors.initials: SC
authors.lastname: Bantele
authors.affiliation: Centre for Medical Biotechnology, Molecular Genetics II, University Duisburg-Essen, Essen, Germany.
authors.forename: Pedro
authors.initials: P
authors.lastname: Ferreira
authors.affiliation: DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Dalia
authors.initials: D
authors.lastname: Gritenaite
authors.affiliation: Centre for Medical Biotechnology, Molecular Genetics II, University Duisburg-Essen, Essen, Germany.
authors.forename: Dominik
authors.initials: D
authors.lastname: Boos
authors.affiliation: DNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Martinsried, Germany.
authors.forename: Boris
authors.identifier: 0000-0003-2180-5054
authors.initials: B
authors.lastname: Pfander
mesh_terms: D002352:Carrier Proteins / Q000378:metabolism; D002453:Cell Cycle; D018797:Cell Cycle Proteins / Q000378:metabolism*; D004265:DNA Helicases / Q000378:metabolism; D004260:DNA Repair; D004261:DNA Replication; D004271:DNA, Fungal / Q000378:metabolism*; D004268:DNA-Binding Proteins / Q000378:metabolism; D006801:Humans; D009687:Nuclear Proteins / Q000378:metabolism; D011995:Recombination, Genetic; D012441:Saccharomyces cerevisiae / Q000201:enzymology / Q000502:physiology*; D029701:Saccharomyces cerevisiae Proteins / Q000378:metabolism*; D014157:Transcription Factors / Q000378:metabolism*
chemical_list: D002352:Carrier Proteins; D018797:Cell Cycle Proteins; D004271:DNA, Fungal; D004268:DNA-Binding Proteins; C097185:DPB11 protein, S cerevisiae; C074000:FUN30 protein, S cerevisiae; D009687:Nuclear Proteins; D029701:Saccharomyces cerevisiae Proteins; C110513:TOPBP1 protein, human; D014157:Transcription Factors; C561641:SMARCAD1 protein, human; D004265:DNA Helicases
keywords: DNA recombination; S. cerevisiae; biochemistry; cell biology; cell cycle; chromatin; double strand breaks; human; phosphorylation