title: Cardioprotective Effect ofUlmus wallichianaPlanchon in β-Adrenergic Agonist Induced Cardiac Hypertrophy.
abstract: Ulmus wallichianaPlanchon (Family: Ulmaceae), a traditional medicinal plant, was used in fracture healing in the folk tradition of Uttarakhand, Himalaya, India. The present study investigated the cardioprotective effect of ethanolic extract (EE) and butanolic fraction (BF) ofU. wallichianain isoprenaline (ISO) induced cardiac hypertrophy in Wistar rats. Cardiac hypertrophy was induced by ISO (5 mg/kg/day, subcutaneously) in rats. Treatment was performed by oral administration of EE and BF ofU. wallichiana(500 and 50 mg/kg/day). The blood pressure (BP) and heart rate (HR) were measured by non-invasive blood pressure measurement technique. Plasma renin, Ang II, NO, and cGMP level were estimated using an ELISA kit. Angiotensin converting enzyme activity was estimated. BP and HR were significantly increased in ISO group (130.33 ± 1.67 mmHg vs. 111.78 ± 1.62 mmHg,p< 0.001 and 450.51 ± 4.90 beats/min vs. 347.82 ± 6.91 beats/min, respectively,p< 0.001). The BP and HR were significantly reduced (EE: 117.53 ± 2.27 mmHg vs. 130.33 ± 1.67 mmHg,p< 0.001, BF: 119.74 ± 3.32 mmHg vs. 130.33 ± 1.67 mmHg,p< 0.001); HR: (EE: 390.22 ± 8.24 beats/min vs. 450.51 ± 4.90 beats/min,p< 0.001, BF: 345.38 ± 6.79 beats/min vs. 450.51 ± 4.90 beats/min,p< 0.001) after the treatment of EE and BF ofU. wallichiana, respectively. Plasma renin, Ang II, ACE activity was decreased and NO, cGMP level were increased. The EE and BF ofU. wallichianadown regulated the expression of ANP, BNP, TNF-α, IL-6, MMP9, β1-AR, TGFβ1 and up regulated NOS3, ACE2 and Mas expression level, respectively. Thus, this study demonstrated thatU. wallichianahas cardioprotective effect against ISO induced cardiac hypertrophy.
authors.affiliation: Division of Pharmacokinetics and Metabolism, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Anees A
authors.initials: AA
authors.lastname: Syed
authors.affiliation: Division of Pharmacokinetics and Metabolism, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Shibani
authors.initials: S
authors.lastname: Lahiri
authors.affiliation: Division of Pharmacology, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Divya
authors.initials: D
authors.lastname: Mohan
authors.affiliation: Division of Pharmacokinetics and Metabolism, Council of Scientific and Industrial Research-Central Drug Research InstituteLucknow, India; Academy of Scientific and Innovative ResearchNew Delhi, India.
authors.forename: Guru R
authors.initials: GR
authors.lastname: Valicherla
authors.affiliation: Division of Pharmacokinetics and Metabolism, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Anand P
authors.initials: AP
authors.lastname: Gupta
authors.affiliation: Division of Medicinal and Process Chemistry, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Sudhir
authors.initials: S
authors.lastname: Kumar
authors.affiliation: Academy of Scientific and Innovative ResearchNew Delhi, India; Division of Medicinal and Process Chemistry, Council of Scientific and Industrial Research-Central Drug Research InstituteLucknow, India.
authors.forename: Rakesh
authors.initials: R
authors.lastname: Maurya
authors.affiliation: Division of Laboratory Animals, Council of Scientific and Industrial Research-Central Drug Research Institute Lucknow, India.
authors.forename: Himanshu K
authors.initials: HK
authors.lastname: Bora
authors.affiliation: Division of Pharmacology, Council of Scientific and Industrial Research-Central Drug Research InstituteLucknow, India; Academy of Scientific and Innovative ResearchNew Delhi, India.
authors.forename: Kashif
authors.initials: K
authors.lastname: Hanif
authors.affiliation: Division of Pharmacokinetics and Metabolism, Council of Scientific and Industrial Research-Central Drug Research InstituteLucknow, India; Academy of Scientific and Innovative ResearchNew Delhi, India.
authors.forename: Jiaur R
authors.initials: JR
authors.lastname: Gayen
keywords: Ulmus wallichiana; blood pressure; cardiac hypertrophy; cardiovascular disease; ethnopharmacology; heart rate; isoprenaline