title: X-ray crystal structure of a malonate-semialdehyde dehydrogenase from Pseudomonas sp. strain AAC.
abstract: The NAD-dependent malonate-semialdehyde dehydrogenase KES23460 from Pseudomonas sp. strain AAC makes up half of a bicistronic operon responsible for β-alanine catabolism to produce acetyl-CoA. The KES23460 protein has been heterologously expressed, purified and used to generate crystals suitable for X-ray diffraction studies. The crystals belonged to space group P212121and diffracted X-rays to beyond 3 Å resolution using the microfocus beamline of the Australian Synchrotron. The structure was solved using molecular replacement, with a monomer from PDB entry 4zz7 as the search model.
authors.affiliation: Land and Water, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Matthew
authors.initials: M
authors.lastname: Wilding
authors.affiliation: Land and Water, CSIRO, GPO Box 1700, Canberra, ACT 2601, Australia.
authors.forename: Colin
authors.initials: C
authors.lastname: Scott
authors.affiliation: Biomedical Program, Manufacturing, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Thomas S
authors.initials: TS
authors.lastname: Peat
authors.affiliation: Biomedical Program, Manufacturing, CSIRO, 343 Royal Parade, Parkville, VIC 3052, Australia.
authors.forename: Janet
authors.initials: J
authors.lastname: Newman
mesh_terms: D000105:Acetyl Coenzyme A / Q000737:chemistry / Q000378:metabolism; D000595:Amino Acid Sequence; D001426:Bacterial Proteins / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D001665:Binding Sites; D003001:Cloning, Molecular; D018360:Crystallography, X-Ray; D004926:Escherichia coli / Q000235:genetics / Q000378:metabolism; D015870:Gene Expression; D050605:Malonate-Semialdehyde Dehydrogenase (Acetylating) / Q000737:chemistry* / Q000235:genetics / Q000378:metabolism; D008958:Models, Molecular; D009243:NAD / Q000737:chemistry* / Q000378:metabolism; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D011485:Protein Binding; D000072756:Protein Conformation, alpha-Helical; D000072757:Protein Conformation, beta-Strand; D054730:Protein Interaction Domains and Motifs; D055503:Protein Multimerization; D011549:Pseudomonas / Q000737:chemistry* / Q000201:enzymology; D011994:Recombinant Proteins / Q000737:chemistry / Q000235:genetics / Q000378:metabolism; D013379:Substrate Specificity; D015091:beta-Alanine / Q000737:chemistry / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D011994:Recombinant Proteins; D009243:NAD; D015091:beta-Alanine; D000105:Acetyl Coenzyme A; D050605:Malonate-Semialdehyde Dehydrogenase (Acetylating)
keywords: Pseudomonas sp. strain AAC; malonate-semialdehyde dehydrogenase; β-alanine catabolism