title: Diversity of the Bacterial Microbiota ofAnophelesMosquitoes from Binh Phuoc Province, Vietnam.
abstract: The naturally acquired microbiota ofAnophelescan influence vector's susceptibility toPlasmodiumand its capacity to transmit them. Microbiota modification is a new challenge to limit disease transmission but it still needs advanced knowledges on bacterial community inAnopheles, especially in wild and infected specimens from diverse origin and species. Bacterial culture and 16S rRNA gene-PCR associated to Temporal Temperature Gradient Electrophoresis (TTGE) were applied to explore the bacterial diversity in the abdomen of 100 wild specimens (eightAnophelesspecies) collected in the Binh Phuoc Province, Vietnam. Culture and PCR-TTGE were complementary. The bacterial richness of the mosquito collection encompassed 105 genera belonging to seven phyla, mostlyProteobacteria,Firmicutes, andActinobacteria.Staphylococcus,Clostridium, andBacillusinFirmicuteswere the most prevalent genera. However,Proteobacteriarepresented by 57 genera was the most diversified phylum inAnophelesmicrobiota. The high overall ofAnopheles-associated bacteria is confirmed with, to our knowledge, 51 genera described for the first time inAnophelesmicrobiota. However, the diversity per specimen was low with average diversity index and the average Shannon-Wiener score (H) of 4.843 and 5.569, respectively. The most represented bacterial genera were present in <30% of the specimens. Consequently, the core microbiota share byAnophelesfrom Binh Phuoc was very narrow, suggesting thatAnophelesmicrobiota was greatly influenced by local environments. The repertory of bacterial genera in two specimens ofAn. dirusandAn. pampanainaturally infected byPlasmodium vivaxwas also described as preliminary results. Finally, this study completed the repertory of bacteria associated to wildAnopheles.Anophelesassociated-bacteria appeared specimen-dependent rather than mosquitoe species- or group-dependent. Their origin and the existence ofAnopheles-specific bacterial taxa are discussed.
authors.affiliation: Institut de Recherche pour le Développement France, UMR-MD3, Faculté de PharmacieMontpellier, France; National Institute of Veterinary ResearchHanoi, Vietnam.
authors.forename: Chung T
authors.initials: CT
authors.lastname: Ngo
authors.affiliation: UMR 5569 Hydrosciences, Equipe Pathogènes Hydriques, Santé et Environnements, Faculté de Pharmacie, Université de MontpellierMontpellier, France; Département d'Hygiène Hospitalière, Centre Hospitalier Universitaire de MontpellierMontpellier, France.
authors.forename: Sara
authors.initials: S
authors.lastname: Romano-Bertrand
authors.affiliation: Institut de Recherche pour le Développement France, UMR-MD3, Faculté de Pharmacie Montpellier, France.
authors.forename: Sylvie
authors.initials: S
authors.lastname: Manguin
authors.affiliation: UMR 5569 Hydrosciences, Equipe Pathogènes Hydriques, Santé et Environnements, Faculté de Pharmacie, Université de MontpellierMontpellier, France; Département d'Hygiène Hospitalière, Centre Hospitalier Universitaire de MontpellierMontpellier, France.
authors.forename: Estelle
authors.initials: E
authors.lastname: Jumas-Bilak
keywords: 16S rRNA PCR – TTGE; Anopheles mosquitoes; Binh Phuoc; Vietnam; abdomen bacterial microbiota; malaria