title: The Antagonistic Effect of Mycotoxins Deoxynivalenol and Zearalenone on Metabolic Profiling in Serum and Liver of Mice.
abstract: Metabolic profiling in liver and serum of mice was studied for the combined toxic effects of deoxynivalenol (DON) and zearalenone (ZEN), through gas chromatography mass spectrum. The spectrum of serum and liver sample of mice, treated with individual 2 mg/kg DON, 20 mg/kg ZEN, and the combined DON + ZEN with final concentration 2 mg/kg DON and 20 mg/kg ZEN for 21 days, were deconvoluted, aligned and identified with MS DIAL. The data matrix was processed with univariate analysis and multivariate analysis for selection of metabolites with variable importance for the projection (VIP) > 1,t-testpvalue < 0.05. The metabolic pathway analysis was performed with MetaMapp and drawn by CytoScape. Results show that the combined DON and ZEN treatment has an obvious "antagonistic effect" in serum and liver tissue metabolic profiling of mice. The blood biochemical indexes, like alkaline phosphatase, alanine transaminase, and albumin (ALB)/globulin (GLO), reveal a moderated trend in the combined DON + ZEN treatment group, which is consistent with histopathological examination. The metabolic pathway analysis demonstrated that the combined DON and ZEN treatment could down-regulate the valine, leucine and isoleucine biosynthesis, glycine, serine and threonine metabolism, andO-glycosyl compounds related glucose metabolism in liver tissue. The metabolic profiling in serum confirmed the finding that the combined DON and ZEN treatment has an "antagonistic effect" on liver metabolism of mice.
authors.affiliation: School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China. jijianjndx@126.com.|Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. jijianjndx@126.com.
authors.forename: Jian
authors.initials: J
authors.lastname: Ji
authors.affiliation: State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Dairy Research Institute, Bright Dairy & Food Co., Ltd., Shanghai 200436, China. peipeiecust@163.com.|Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. peipeiecust@163.com.
authors.forename: Pei
authors.initials: P
authors.lastname: Zhu
authors.affiliation: School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China. cfc1031@163.com.|Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. cfc1031@163.com.
authors.forename: Fangchao
authors.initials: F
authors.lastname: Cui
authors.affiliation: School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China. pifuwei@hotmail.com.|Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. pifuwei@hotmail.com.
authors.forename: Fuwei
authors.initials: F
authors.lastname: Pi
authors.affiliation: School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China. yinzhizhang@jiangnan.edu.cn.|Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. yinzhizhang@jiangnan.edu.cn.
authors.forename: Yinzhi
authors.initials: Y
authors.lastname: Zhang
authors.affiliation: School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China. gz-liyun@126.com.|Key Laboratory of Agro-Food Safety and Quality of Ministry of Agriculture, Institute of Quality Standards and Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, No. 12, Zhongguancun South Street, Beijing 100081, China. gz-liyun@126.com.
authors.forename: Yun
authors.initials: Y
authors.lastname: Li
authors.affiliation: School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China. jswang@uga.edu.|Department of Environmental Health Science, College of Public Health, University of Georgia, Athens, GA 30602, USA. jswang@uga.edu.
authors.forename: Jiasheng
authors.initials: J
authors.lastname: Wang
authors.affiliation: School of Food Science, State Key Laboratory of Food Science and Technology, National Engineering Research Center for Functional Foods, Jiangnan University, Wuxi 214122, China. sxlzyz@jiangnan.edu.cn.|Synergetic Innovation Center for Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China. sxlzyz@jiangnan.edu.cn.
authors.forename: Xiulan
authors.initials: X
authors.lastname: Sun
mesh_terms: D000410:Alanine Transaminase / Q000097:blood*; D000469:Alkaline Phosphatase / Q000097:blood*; D000596:Amino Acids / Q000378:metabolism; D000818:Animals; D015415:Biomarkers / Q000097:blood; D004336:Drug Antagonism; D004357:Drug Synergism; D005260:Female; D008099:Liver / Q000187:drug effects* / Q000378:metabolism / Q000473:pathology; D008111:Liver Function Tests; D008297:Male; D055442:Metabolome / Q000187:drug effects*; D008815:Mice, Inbred Strains; D014255:Trichothecenes / Q000633:toxicity*; D015025:Zearalenone / Q000633:toxicity*
chemical_list: D000596:Amino Acids; D015415:Biomarkers; D014255:Trichothecenes; D015025:Zearalenone; D000410:Alanine Transaminase; D000469:Alkaline Phosphatase; C007262:deoxynivalenol
keywords: antagonistic effect; combined toxicity; deoxynivalenol; metabolomics; zearalenone