title: Nup82 functions redundantly with Nup136 in a salicylic acid-dependent defense response of Arabidopsis thaliana.
abstract: The nuclear pore complex (NPC) comprises more than 30 nucleoporins (Nups). NPC mediates macromolecular trafficking between the nucleoplasm and the cytoplasm, but specific roles of individual Nups are poorly understood in higher plants. Here, we show that the novel nucleoporin unique to angiosperm plants (designated as Nup82) functions in a salicylic acid-dependent defense in a redundant manner with Nup136, which is a component of the nuclear basket in the NPC. Arabidopsis thaliana Nup82 had a similar amino acid sequence to the N-terminal half of Nup136 and a Nup82-GFP fusion was localized on the nuclear envelope. Immunoprecipitation and bimolecular fluorescence complementation analyses revealed that Nup82 interacts with the NPC components Nup136 and RAE1. The double knockout mutant nup82 nup136 showed severe growth defects, while the single knockout mutant nup82 did not, suggesting that Nup82 functions redundantly with Nup136. nup82 nup136 impaired benzothiadiazole (an analog of salicylic acid)-induced resistance to the virulent bacteria Pseudomonas syringae pv. tomato DC3000. Furthermore, transcriptome analysis of nup82 nup136 indicates that deficiency of Nup82 and Nup136 causes noticeable downregulation of immune-related genes. These results suggest that Nup82 and Nup136 are redundantly involved in transcriptional regulation of salicylic acid-responsive genes through nuclear transport of signaling molecules.
authors.affiliation: a Department of Botany , Graduate School of Science, Kyoto University , Kyoto , Japan.
authors.forename: Kentaro
authors.identifier: 0000-0001-7387-2211
authors.initials: K
authors.lastname: Tamura
authors.affiliation: b Department of Bioinformatics , College of Life Sciences, Ritsumeikan University , Shiga , Japan.
authors.forename: Yoichiro
authors.initials: Y
authors.lastname: Fukao
authors.affiliation: c Department of Plant Biology , Microbial and Plant Genomics Institute, University of Minnesota , St. Paul , MN , USA.
authors.forename: Noriyuki
authors.identifier: 0000-0002-5708-4263
authors.initials: N
authors.lastname: Hatsugai
authors.affiliation: c Department of Plant Biology , Microbial and Plant Genomics Institute, University of Minnesota , St. Paul , MN , USA.
authors.forename: Fumiaki
authors.identifier: 0000-0001-6893-3788
authors.initials: F
authors.lastname: Katagiri
authors.affiliation: a Department of Botany , Graduate School of Science, Kyoto University , Kyoto , Japan.
authors.forename: Ikuko
authors.identifier: 0000-0001-8814-1593
authors.initials: I
authors.lastname: Hara-Nishimura
mesh_terms: D017360:Arabidopsis / Q000235:genetics / Q000254:growth & development / Q000276:immunology* / Q000378:metabolism*; D029681:Arabidopsis Proteins / Q000235:genetics / Q000378:metabolism*; D018506:Gene Expression Regulation, Plant; D055786:Gene Knockout Techniques; D028861:Nuclear Pore Complex Proteins / Q000235:genetics / Q000378:metabolism*; D020156:Salicylic Acid / Q000378:metabolism*
chemical_list: D029681:Arabidopsis Proteins; D028861:Nuclear Pore Complex Proteins; C000626315:Nup136 protein, Arabidopsis; C000626314:Nup82 protein, Arabidopsis; D020156:Salicylic Acid
keywords: Arabidopsis thaliana; Nup136/Nup1; Nup82; nuclear envelope; nucleoporin; plant immunity; proteome; transcriptome