title: Two-Step Assembling of Near-Infrared "OFF-ON" Fluorescent Nanohybrids for Synchronous Tumor Imaging and MicroRNA Modulation-Based Therapy.
abstract: Theranostic nanoparticles with combined imaging and therapy functions show great promise in cancer precision medicine. In this study, we constructed near-infrared (NIR) "OFF-ON" fluorescent nanohybrids (F-PNDs) for synchronous tumor imaging and microRNA (miRNA) modulation therapy against esophageal cancer. Nanodiamond clusters (NDs) were first functionalized for protamine sulfate immobilization (PNDs) on their surfaces via a noncovalent self-assembling approach and simultaneous encapsulation of NIR emitting fluorescence dye cyanine 5 (Cy-5) (F-PNDs). Tumor suppressor miRNA-203 (miR-203) was then adsorbed onto the surface of F-PNDs to form miR-203/F-PNDs via electrostatic interactions. The size, morphology, photophysical and stability properties of miR-203/F-PNDs were analyzed. We found that the NIR fluorescence of miR-203/F-PNDs could be activated to the "ON" state in intracellular environment while remaining in the "OFF" state in extracellular or blood environment. Furthermore, in vivo live imaging experiments showed that miR-203/F-PNDs could be predominantly accumulated in tumor tissues and image the tumor sites 24 h postintravenous injection. In addition, intravenous and intratumoral injection of miR-203/F-PNDs could efficiently inhibit tumor growth through down-regulation of the expressions of oncogenes Ran and Δp63. Our study indicated that miRNA/F-PNDs could serve as a promising theranostic platform for synchronous tumor imaging and miRNA-based modulation therapy against cancer.
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.|CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
authors.forename: Xiongwei
authors.initials: X
authors.lastname: Deng
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Zhaoxia
authors.initials: Z
authors.lastname: Yin
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
authors.forename: Jianqing
authors.initials: J
authors.lastname: Lu
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Yang
authors.initials: Y
authors.lastname: Xia
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
authors.forename: Leihou
authors.initials: L
authors.lastname: Shao
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Qin
authors.initials: Q
authors.lastname: Hu
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Zhixiang
authors.initials: Z
authors.lastname: Zhou
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Fang
authors.initials: F
authors.lastname: Zhang
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Shaomei
authors.initials: S
authors.lastname: Zhou
authors.affiliation: CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology , No. 11 Beiyitiao, Zhongguancun, Beijing 100190, China.
authors.forename: Yan
authors.identifier: http://orcid.org/0000-0001-8508-7077
authors.initials: Y
authors.lastname: Wu
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Wang
authors.identifier: http://orcid.org/0000-0003-4042-5102
authors.initials: W
authors.lastname: Sheng
authors.affiliation: College of Life Science and Bioengineering, Beijing University of Technology , No. 100 Pingleyuan, Chaoyang District, Beijing 100124, P.R. China.
authors.forename: Yi
authors.initials: Y
authors.lastname: Zeng
mesh_terms: D045744:Cell Line, Tumor; D005453:Fluorescence; D005456:Fluorescent Dyes; D006801:Humans; D035683:MicroRNAs / Q000235:genetics*; D053758:Nanoparticles; D009378:Neoplasms, Multiple Primary; D000068936:Theranostic Nanomedicine
chemical_list: D005456:Fluorescent Dyes; C546703:MIRN203 microRNA, human; D035683:MicroRNAs
keywords: NIR fluorescence; microRNA modulation therapy; nanohybrids; tumor imaging; “OFF−ON”