title: Insights into nuclear dynamics using live-cell imaging approaches.
abstract: The nucleus contains the genetic blueprint of the cell and myriad interactions within this subcellular structure are required for gene regulation. In the current scientific era, characterization of these gene regulatory networks through biochemical techniques coupled with systems-wide 'omic' approaches has become commonplace. However, these strategies are limited because they represent a mere snapshot of the cellular state. To obtain a holistic understanding of nuclear dynamics, relevant molecules must be studied in their native contexts in living systems. Live-cell imaging approaches are capable of providing quantitative assessment of the dynamics of gene regulatory interactions within the nucleus. We survey recent insights into what live-cell imaging approaches have provided the field of nuclear dynamics. In this review, we focus on interactions of DNA with other DNA loci, proteins, RNA, and the nuclear envelope. WIREs Syst Biol Med 2017, 9:e1372. doi: 10.1002/wsbm.1372 For further resources related to this article, please visit the WIREs website.
authors.affiliation: Cardiovascular Research Institute and Department of Physiology, School of Medicine, University of California, San Francisco, CA, USA.
authors.forename: Rachel B
authors.initials: RB
authors.lastname: Bigley
authors.affiliation: Cardiovascular Research Institute and Department of Physiology, School of Medicine, University of California, San Francisco, CA, USA.
authors.forename: Alexander Y
authors.initials: AY
authors.lastname: Payumo
authors.affiliation: Cardiovascular Research Institute and Department of Physiology, School of Medicine, University of California, San Francisco, CA, USA.
authors.forename: Jeffrey M
authors.initials: JM
authors.lastname: Alexander
authors.affiliation: Cardiovascular Research Institute and Department of Physiology, School of Medicine, University of California, San Francisco, CA, USA.
authors.forename: Guo N
authors.initials: GN
authors.lastname: Huang
mesh_terms: D002467:Cell Nucleus / Q000378:metabolism*; D004247:DNA / Q000737:chemistry / Q000378:metabolism; D008954:Models, Biological; D009685:Nuclear Envelope / Q000737:chemistry / Q000378:metabolism; D009687:Nuclear Proteins / Q000737:chemistry / Q000378:metabolism; D012313:RNA / Q000737:chemistry / Q000378:metabolism
chemical_list: D009687:Nuclear Proteins; D012313:RNA; D004247:DNA