title: Recombinant hepatocyte growth factor activator inhibitor 1: expression in Drosophila S2 cells, purification and crystallization.
abstract: Hepatocyte growth factor activator inhibitor 1 (HAI-1) is a multi-domain membrane-associated protease inhibitor that potently inhibits a variety of serine proteases such as hepatocyte growth factor activator and matriptase. Different truncates of HAI-1 show varying potencies for inhibition of target proteases, suggesting that the domain organization of HAI-1 plays a critical role in its function. Here, the soluble full-length extracellular part of HAI-1 (sHAI-1) was expressed using the Drosophila S2 insect-cell expression system. Diffraction-quality crystals of sHAI-1 were produced using ammonium sulfate as precipitant. The crystal diffracted to 3.8 Å resolution and belonged to space group P41212, with unit-cell parameters a = b = 95.42, c = 124.50 Å. The asymmetric unit contains one sHAI-1 molecule.
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Min
authors.initials: M
authors.lastname: Liu
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Cai
authors.initials: C
authors.lastname: Yuan
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Yunbin
authors.initials: Y
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Longguang
authors.initials: L
authors.lastname: Jiang
authors.affiliation: State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Science, Fuzhou, Fujian 350002, People's Republic of China.
authors.forename: Mingdong
authors.initials: M
authors.lastname: Huang
mesh_terms: D000595:Amino Acid Sequence; D000645:Ammonium Sulfate / Q000737:chemistry; D000818:Animals; D011232:Chemical Precipitation; D003460:Crystallization; D018360:Crystallography, X-Ray; D004331:Drosophila melanogaster / Q000166:cytology / Q000378:metabolism; D015870:Gene Expression; D006801:Humans; D010957:Plasmids / Q000737:chemistry / Q000378:metabolism; D053491:Proteinase Inhibitory Proteins, Secretory / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D011993:Recombinant Fusion Proteins / Q000737:chemistry / Q000235:genetics* / Q000378:metabolism; D014961:X-Ray Diffraction
chemical_list: D053491:Proteinase Inhibitory Proteins, Secretory; D011993:Recombinant Fusion Proteins; C109149:SPINT1 protein, human; D000645:Ammonium Sulfate
keywords: Drosophila; HAI-1; crystallization; expression; hepatocyte growth factor activator inhibitor; purification