title: The Denture-Associated Oral Microbiome in Health and Stomatitis.
abstract: While investigation of the microbiome on natural oral surfaces has generated a wealth of information, few studies have examined the microbial communities colonizing dentures and their relationship to oral health. To address this knowledge gap, we characterized the bacterial community associated with dentures and remaining teeth in healthy individuals and patients with denture stomatitis. The microbiome compositions of matched denture and tooth plaque samples of 10 healthy individuals and 9 stomatitis patients were determined by 16S rRNA gene pyrosequencing. The microbial communities colonizing dentures and remaining teeth in health and disease were very similar to each other. Matched denture and tooth samples from the same individuals shared a significantly higher percentage of identical phylotypes than random pairs of samples from different study participants. Despite these overall similarities, several bacterial phylotypes displayed discrete health- and stomatitis-associated denture colonization, while others were distinct in health and disease independently of the surface. Certain phylotypes exhibited differential colonization of dentures and teeth independently of denture health status. In conclusion, denture and natural tooth surfaces in health and stomatitis harbor similar bacterial communities. Individual-related rather than surface-specific factors play a significant role in the bacterial phylotype composition colonizing dentures and teeth. This individual-specific mutual influence on denture and tooth surface colonization could be an important factor in maintaining oral health in denture wearers. Discrete differences in colonization patterns for distinct genera and phylotypes warrant further studies regarding their potential involvement or utility as specific indicators of health and disease development in denture-wearing individuals.IMPORTANCEDenture stomatitis is a prevalent inflammatory condition of the mucosal tissue in denture wearers that is triggered by microorganisms. WhileCandidahas been extensively studied for its role in stomatitis etiology, the bacterial component largely remains to be investigated. Our data show that certain types of bacteria are significantly associated with denture health and disease. Furthermore, the bacterial communities residing on the teeth and dentures of the same person are similar to each other independently of the surface, and thus, denture health could impact the maintenance of remaining teeth and vice versa.
authors.affiliation: Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of Los Angeles at Los Angeles, Los Angeles, California, USA.
authors.forename: Baochen
authors.initials: B
authors.lastname: Shi
authors.affiliation: School of Dentistry, University of Los Angeles at Los Angeles, Los Angeles, California, USA.
authors.forename: Tingxi
authors.initials: T
authors.lastname: Wu
authors.affiliation: Department of Periodontics, School of Dentistry, University of Washington, Seattle, Washington, USA.
authors.forename: Jeffrey
authors.initials: J
authors.lastname: McLean
authors.affiliation: School of Dentistry, University of Los Angeles at Los Angeles, Los Angeles, California, USA; Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, California, USA.
authors.forename: Anna
authors.initials: A
authors.lastname: Edlund
authors.affiliation: Microbial and Environmental Genomics, J. Craig Venter Institute, La Jolla, California, USA.
authors.forename: Youngik
authors.initials: Y
authors.lastname: Young
authors.affiliation: School of Dentistry, University of Los Angeles at Los Angeles, Los Angeles, California, USA.
authors.forename: Xuesong
authors.initials: X
authors.lastname: He
authors.affiliation: West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Hongyang
authors.initials: H
authors.lastname: Lv
authors.affiliation: West China School of Stomatology, Sichuan University, Chengdu, Sichuan, China.
authors.forename: Xuedong
authors.initials: X
authors.lastname: Zhou
authors.affiliation: School of Dentistry, University of Los Angeles at Los Angeles, Los Angeles, California, USA.
authors.forename: Wenyuan
authors.initials: W
authors.lastname: Shi
authors.affiliation: Department of Molecular and Medical Pharmacology, Crump Institute for Molecular Imaging, David Geffen School of Medicine, University of Los Angeles at Los Angeles, Los Angeles, California, USA.
authors.forename: Huiying
authors.initials: H
authors.lastname: Li
authors.affiliation: School of Dentistry, University of Los Angeles at Los Angeles, Los Angeles, California, USA.
authors.forename: Renate
authors.initials: R
authors.lastname: Lux
keywords: bacteria; denture; microbiome; stomatitis