title: TsdC, a unique lipoprotein from Wolinella succinogenes that enhances tetrathionate reductase activity of TsdA.
abstract: The diheme cytochromes c of the widespread TsdA family are bifunctional thiosulfate dehydrogenase/tetrathionate reductases. Here, biochemical information was collected about TsdA from the Epsilonproteobacterium Wolinella succinogenes (WsTsdA). The situation in W. succinogenes is unique since TsdA is closely associated with the unprecedented lipoprotein TsdC encoded immediately downstream of tsdA in the same direction of transcription. WsTsdA purified from Escherichia coli catalyzed both thiosulfate oxidation and tetrathionate reduction. After co-production of TsdC and WsTsdA in E. coli, TsdC was found to mediate membrane attachment of TsdA and to ensure its full catalytic activity. This effect was much stronger in the tetrathionate-reducing than in the thiosulfate-oxidizing direction. It is concluded that the TsdAC complex predominantly acts as a tetrathionate reductase in vivo.
authors.affiliation: Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, D-53115 Bonn, Germany.
authors.forename: Julia M
authors.initials: JM
authors.lastname: Kurth
authors.affiliation: Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, D-53115 Bonn, Germany.
authors.forename: Anja
authors.initials: A
authors.lastname: Schuster
authors.affiliation: Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, D-53115 Bonn, Germany.
authors.forename: Waldemar
authors.initials: W
authors.lastname: Seel
authors.affiliation: Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, D-53115 Bonn, Germany.
authors.forename: Stefanie
authors.initials: S
authors.lastname: Herresthal
authors.affiliation: Microbial Energy Conversion and Biotechnology, Fachbereich Biologie, Technische Universität Darmstadt, D-64287 Darmstadt, Germany.
authors.forename: Jörg
authors.initials: J
authors.lastname: Simon
authors.affiliation: Institut für Mikrobiologie & Biotechnologie, Rheinische Friedrich-Wilhelms-Universität Bonn, D-53115 Bonn, Germany.
authors.forename: Christiane
authors.initials: C
authors.lastname: Dahl
mesh_terms: D001426:Bacterial Proteins / Q000378:metabolism*; D055162:Biocatalysis; D004926:Escherichia coli / Q000378:metabolism; D008074:Lipoproteins / Q000302:isolation & purification / Q000378:metabolism*; D010084:Oxidation-Reduction; D010088:Oxidoreductases / Q000378:metabolism*; D016968:Wolinella / Q000737:chemistry* / Q000201:enzymology* / Q000378:metabolism
chemical_list: D001426:Bacterial Proteins; D008074:Lipoproteins; D010088:Oxidoreductases; C022727:tetrathionate reductase; C090099:diheme cytochrome c; C432587:thiosulfate dehydrogenase
keywords: TsdA; Wolinella succinogenes; cytochrome c; lipoprotein; tetrathionate reductase; thiosulfate dehydrogenase