title: Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1).
abstract: In the present work, controlled experimental infection and transmission studies in domestic cattle (Bos taurus) and water buffaloes (Bubalus bubalis) were carried out to study the in vivo behaviour of bubaline herpesvirus 1 (BuHV1). Two bovine and two buffalo calves were infected with BuHV1 (20287N isolate) by intranasal aerosolisation. Two sentinel cattle did not receive the virus challenge, but were housed with infected buffaloes to evaluate horizontal transmission. All experimentally inoculated animals showed viral infection and respiratory clinical signs. BuHV1 experimentally infected calves showed intermittent viral excretion between 2 days and 18 days postinfection (dpi) with a maximum titre of excretion of 106TCID50/ml and moderate rhinitis between 2 dpi and 20 dpi. BuHV1 experimentally inoculated buffaloes showed mild respiratory signs, which consisted mainly of serous nasal secretions during the infection period. Sentinel calves showed mucosal specific IgG1antibodies at seven days postcontact. Viral DNA was detected by PCR and sequencing in both buffaloes and sentinel calves, which could be associated with latency. In conclusion, this study showed the susceptibility of cattle to BuHV1 after both experimental infection and contact with infected buffaloes. These data increase the scarce knowledge on the pathogenesis in natural host and the susceptibility of cattle to BuHV1 experimental infection.
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917 (C1033AAJ), Ciudad Autónoma de Buenos Aires, Argentina; Cátedra de Inmunogenética, Facultad de Ciencias exactas, Químicas y Naturales, Universidad de Morón, Morón, Buenos Aires, Argentina.
authors.forename: S S
authors.initials: SS
authors.lastname: Maidana
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA) , Castelar, Buenos Aires , Argentina.
authors.forename: F
authors.initials: F
authors.lastname: Delgado
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA) , Castelar, Buenos Aires , Argentina.
authors.forename: L
authors.initials: L
authors.lastname: Vagnoni
authors.affiliation: Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research on Animal Health center and Faculty of Veterinary Medicine, University of Liège , Liège , Belgium.
authors.forename: A
authors.initials: A
authors.lastname: Mauroy
authors.affiliation: Veterinary Virology and Animal Viral Diseases, Fundamental and Applied Research on Animal Health center and Faculty of Veterinary Medicine, University of Liège , Liège , Belgium.
authors.forename: E
authors.initials: E
authors.lastname: Thiry
authors.affiliation: Instituto de Virología, Centro de Investigaciones en Ciencias Veterinarias y Agronómicas (CICVyA), Instituto de tecnología Agropecuaria (INTA), Castelar, Buenos Aires, Argentina; Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917 (C1033AAJ), Ciudad Autónoma de Buenos Aires, Argentina; Cátedra de Inmunogenética, Facultad de Ciencias exactas, Químicas y Naturales, Universidad de Morón, Morón, Buenos Aires, Argentina; Cátedra de Inmunología, Universidad del Salvador, Pilar, Provincia de Buenos Aires, Argentina.
authors.forename: S
authors.initials: S
authors.lastname: Romera
keywords: BuHV1; Buffaloes; Cattle; Transmission