title: Melanoma-Induced Anemia Could be Rescued by Sca-1+Mesenchymal Stromal Cells in Mice.
abstract: The intrinsic basis of cancer-related anemia (CRA) is erythropoiesis disorder, which is a common complication of cancer and exerts a negative influence on the life quality of cancer patients. Cell therapy using mesenchymal stromal cells (MSCs) is considered as a promising method in cancer treatment. Furthermore, MSCs have been used to cure few type of anemia and be considered as a potential strategy to recover anemia radically. However, none reports its application in CRA treatment. In CRA model mice, we found that the number of lin-c-kit+Sca-1+and Sca-1+MSCs was decreased. And CRA resulted in an increased number of proerythroblasts and basophilic erythroblasts and decreased number of orthochromatic erythroblasts. Furthermore, in CRA model mice transplanted with Sca-1+MSCs and MSCs, the levels of red blood cell count and Hb in peripheral blood were obviously increased. And the accumulation of proerythroblasts and basophilic erythroblasts was inhibited. In addition, the expression patterns of GATA-1 and GATA-2, which is pivotal to anemia, were remarkably recovered. Our results demonstrated that either MSCs or its subpopulation could effectively recover CRA erythropoiesis through GATA-1/GATA-2 signaling, which outstrips the traditional symptomatic therapy.
authors.affiliation: 1 Department of Transfusion Medicine, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Ning
authors.initials: N
authors.lastname: An
authors.affiliation: 1 Department of Transfusion Medicine, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Yaozhen
authors.initials: Y
authors.lastname: Chen
authors.affiliation: 2 Department of Hematology, Tangdu Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Dandan
authors.initials: D
authors.lastname: Yin
authors.affiliation: 1 Department of Transfusion Medicine, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Hui-Jie
authors.initials: HJ
authors.lastname: Zhang
authors.affiliation: 3 Department of Biochemistry and Molecular Biophysics, Columbia University , New York, New York.
authors.forename: Zheng
authors.initials: Z
authors.lastname: Liu
authors.affiliation: 4 Department of Digestive Surgery, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Fan
authors.initials: F
authors.lastname: Feng
authors.affiliation: 1 Department of Transfusion Medicine, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Na
authors.initials: N
authors.lastname: Li
authors.affiliation: 1 Department of Transfusion Medicine, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Jiajia
authors.initials: J
authors.lastname: Xin
authors.affiliation: 1 Department of Transfusion Medicine, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Wen
authors.initials: W
authors.lastname: Yin
authors.affiliation: 5 Molecular Biology Center, State Key Laboratory of Trauma, Burn, and Combined Injury, Research Institute of Surgery and Daping Hospital, Third Military Medical University , Chongqing, China .
authors.forename: Xueqing
authors.initials: X
authors.lastname: Xu
authors.affiliation: 1 Department of Transfusion Medicine, Xijing Hospital, The Fourth Military Medical University , Xi'an, China .
authors.forename: Xingbin
authors.initials: X
authors.lastname: Hu
mesh_terms: D000740:Anemia / Q000209:etiology*; D000818:Animals; D004195:Disease Models, Animal; D004900:Erythroblasts / Q000166:cytology*; D004920:Erythropoiesis / Q000502:physiology*; D008545:Melanoma / Q000150:complications* / Q000473:pathology; D045164:Mesenchymal Stem Cell Transplantation / Q000379:methods; D059630:Mesenchymal Stem Cells / Q000378:metabolism*; D008810:Mice, Inbred C57BL
keywords: MSCs; Sca-1+ MSC subpopulation; cancer-related anemia; erythropoiesis; melanoma